LOCUS       DAEPOG010000001        69421 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEPOG010000001 DAEPOG010000000
VERSION     DAEPOG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.001"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            26..931
                     /locus_tag="KR432_000001"
     CDS             26..931
                     /locus_tag="KR432_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBH0638566.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            940..2124
                     /locus_tag="KR432_000002"
     CDS             940..2124
                     /locus_tag="KR432_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0638567.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            2239..3540
                     /locus_tag="KR432_000003"
     CDS             2239..3540
                     /locus_tag="KR432_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH0638568.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            3494..4537
                     /gene="holA"
                     /locus_tag="KR432_000004"
     CDS             3494..4537
                     /gene="holA"
                     /locus_tag="KR432_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH0638569.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(4724..4990)
                     /gene="rpsT"
                     /locus_tag="KR432_000005"
     CDS             complement(4724..4990)
                     /gene="rpsT"
                     /locus_tag="KR432_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH0638570.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            5589..6110
                     /locus_tag="KR432_000006"
     CDS             5589..6110
                     /locus_tag="KR432_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0638571.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            6144..6920
                     /locus_tag="KR432_000007"
     CDS             6144..6920
                     /locus_tag="KR432_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0638572.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            7263..8216
                     /locus_tag="KR432_000008"
     CDS             7263..8216
                     /locus_tag="KR432_000008"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBH0638573.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            8234..9253
                     /locus_tag="KR432_000009"
     CDS             8234..9253
                     /locus_tag="KR432_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBH0638574.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDKY
                     FKQKN"
     gene            9284..9535
                     /locus_tag="KR432_000010"
     CDS             9284..9535
                     /locus_tag="KR432_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638575.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            9817..11622
                     /gene="lepA"
                     /locus_tag="KR432_000011"
     CDS             9817..11622
                     /gene="lepA"
                     /locus_tag="KR432_000011"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH0638576.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            11773..13110
                     /locus_tag="KR432_000012"
     CDS             11773..13110
                     /locus_tag="KR432_000012"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBH0638577.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            13343..14656
                     /locus_tag="KR432_000013"
     CDS             13343..14656
                     /locus_tag="KR432_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0638578.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            14828..16003
                     /locus_tag="KR432_000014"
     CDS             14828..16003
                     /locus_tag="KR432_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH0638579.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            16106..17125
                     /gene="hrcA"
                     /locus_tag="KR432_000015"
     CDS             16106..17125
                     /gene="hrcA"
                     /locus_tag="KR432_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBH0638580.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            17155..17775
                     /gene="grpE"
                     /locus_tag="KR432_000016"
     CDS             17155..17775
                     /gene="grpE"
                     /locus_tag="KR432_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH0638581.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            17867..19714
                     /gene="dnaK"
                     /locus_tag="KR432_000017"
     CDS             17867..19714
                     /gene="dnaK"
                     /locus_tag="KR432_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH0638582.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            19925..21079
                     /gene="dnaJ"
                     /locus_tag="KR432_000018"
     CDS             19925..21079
                     /gene="dnaJ"
                     /locus_tag="KR432_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH0638583.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            22045..22980
                     /locus_tag="KR432_000019"
     CDS             22045..22980
                     /locus_tag="KR432_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0638584.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            23016..24389
                     /locus_tag="KR432_000020"
     CDS             23016..24389
                     /locus_tag="KR432_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0638585.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(24577..25344)
                     /locus_tag="KR432_000021"
     CDS             complement(24577..25344)
                     /locus_tag="KR432_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0638586.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(25374..26483)
                     /gene="ugpC"
                     /locus_tag="KR432_000022"
     CDS             complement(25374..26483)
                     /gene="ugpC"
                     /locus_tag="KR432_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBH0638587.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            26742..27479
                     /gene="cas6"
                     /locus_tag="KR432_000023"
     CDS             26742..27479
                     /gene="cas6"
                     /locus_tag="KR432_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0638588.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            27484..28959
                     /locus_tag="KR432_000024"
     CDS             27484..28959
                     /locus_tag="KR432_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638589.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            28979..29992
                     /locus_tag="KR432_000025"
     CDS             28979..29992
                     /locus_tag="KR432_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0638590.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            30008..30814
                     /gene="cas5"
                     /locus_tag="KR432_000026"
     CDS             30008..30814
                     /gene="cas5"
                     /locus_tag="KR432_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0638591.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            30841..33249
                     /locus_tag="KR432_000027"
     CDS             30841..33249
                     /locus_tag="KR432_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0638592.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            33690..34637
                     /gene="prmA"
                     /locus_tag="KR432_000028"
     CDS             33690..34637
                     /gene="prmA"
                     /locus_tag="KR432_000028"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH0638593.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            34656..35414
                     /locus_tag="KR432_000029"
     CDS             34656..35414
                     /locus_tag="KR432_000029"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH0638594.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            35416..36714
                     /gene="mtaB"
                     /locus_tag="KR432_000030"
     CDS             35416..36714
                     /gene="mtaB"
                     /locus_tag="KR432_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBH0638595.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            36757..37107
                     /locus_tag="KR432_000031"
     CDS             36757..37107
                     /locus_tag="KR432_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBH0638596.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            37270..37449
                     /locus_tag="KR432_000032"
     CDS             37270..37449
                     /locus_tag="KR432_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH0638597.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            37479..37922
                     /locus_tag="KR432_000033"
     CDS             37479..37922
                     /locus_tag="KR432_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBH0638598.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            38033..38530
                     /locus_tag="KR432_000034"
     CDS             38033..38530
                     /locus_tag="KR432_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBH0638599.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            38723..39286
                     /gene="raiA"
                     /locus_tag="KR432_000035"
     CDS             38723..39286
                     /gene="raiA"
                     /locus_tag="KR432_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0638600.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            39499..39735
                     /locus_tag="KR432_000036"
     CDS             39499..39735
                     /locus_tag="KR432_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBH0638601.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            39748..41091
                     /gene="yqfD"
                     /locus_tag="KR432_000037"
     CDS             39748..41091
                     /gene="yqfD"
                     /locus_tag="KR432_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBH0638602.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            41030..42145
                     /locus_tag="KR432_000038"
     CDS             41030..42145
                     /locus_tag="KR432_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH0638603.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            42202..42663
                     /gene="ybeY"
                     /locus_tag="KR432_000039"
     CDS             42202..42663
                     /gene="ybeY"
                     /locus_tag="KR432_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH0638604.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            42669..43388
                     /locus_tag="KR432_000040"
     CDS             42669..43388
                     /locus_tag="KR432_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH0638605.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            43507..43908
                     /locus_tag="KR432_000041"
     CDS             43507..43908
                     /locus_tag="KR432_000041"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0638606.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            43972..44865
                     /gene="era"
                     /locus_tag="KR432_000042"
     CDS             43972..44865
                     /gene="era"
                     /locus_tag="KR432_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH0638607.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            44877..46256
                     /gene="mgtE"
                     /locus_tag="KR432_000043"
     CDS             44877..46256
                     /gene="mgtE"
                     /locus_tag="KR432_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBH0638608.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            46329..46484
                     /locus_tag="KR432_000044"
     CDS             46329..46484
                     /locus_tag="KR432_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBH0638609.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            46450..47223
                     /gene="recO"
                     /locus_tag="KR432_000045"
     CDS             46450..47223
                     /gene="recO"
                     /locus_tag="KR432_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH0638610.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            47216..47815
                     /locus_tag="KR432_000046"
     CDS             47216..47815
                     /locus_tag="KR432_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBH0638611.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            47975..48853
                     /gene="glyQ"
                     /locus_tag="KR432_000047"
     CDS             47975..48853
                     /gene="glyQ"
                     /locus_tag="KR432_000047"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH0638612.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            48853..50919
                     /locus_tag="KR432_000048"
     CDS             48853..50919
                     /locus_tag="KR432_000048"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH0638613.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(51085..52632)
                     /locus_tag="KR432_000049"
     CDS             complement(51085..52632)
                     /locus_tag="KR432_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBH0638614.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            52912..53679
                     /locus_tag="KR432_000050"
     CDS             52912..53679
                     /locus_tag="KR432_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0638615.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            53962..54159
                     /locus_tag="KR432_000051"
     CDS             53962..54159
                     /locus_tag="KR432_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0638616.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            54188..55264
                     /gene="vorB"
                     /locus_tag="KR432_000052"
     CDS             54188..55264
                     /gene="vorB"
                     /locus_tag="KR432_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0638617.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            55267..55986
                     /locus_tag="KR432_000053"
     CDS             55267..55986
                     /locus_tag="KR432_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0638618.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            55999..56538
                     /locus_tag="KR432_000054"
     CDS             55999..56538
                     /locus_tag="KR432_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0638619.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            56695..57753
                     /gene="buk"
                     /locus_tag="KR432_000055"
     CDS             56695..57753
                     /gene="buk"
                     /locus_tag="KR432_000055"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0638620.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            57771..58664
                     /locus_tag="KR432_000056"
     CDS             57771..58664
                     /locus_tag="KR432_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0638621.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            58694..59902
                     /locus_tag="KR432_000057"
     CDS             58694..59902
                     /locus_tag="KR432_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0638622.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            59926..61188
                     /locus_tag="KR432_000058"
     CDS             59926..61188
                     /locus_tag="KR432_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0638623.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            61764..63227
                     /locus_tag="KR432_000059"
     CDS             61764..63227
                     /locus_tag="KR432_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0638624.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            63283..64419
                     /locus_tag="KR432_000060"
     CDS             63283..64419
                     /locus_tag="KR432_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0638625.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            64655..65770
                     /locus_tag="KR432_000061"
     CDS             64655..65770
                     /locus_tag="KR432_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBH0638626.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            65922..66791
                     /locus_tag="KR432_000062"
     CDS             65922..66791
                     /locus_tag="KR432_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0638627.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            67167..67712
                     /locus_tag="KR432_000063"
     CDS             67167..67712
                     /locus_tag="KR432_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0638628.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            67725..68738
                     /locus_tag="KR432_000064"
     CDS             67725..68738
                     /locus_tag="KR432_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0638629.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            68960..>69421
                     /locus_tag="KR432_000065"
     CDS             68960..>69421
                     /locus_tag="KR432_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0638630.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNT"
BASE COUNT        27536 a         7270 c        12543 g        22072 t
ORIGIN      
        1 gcaataaaaa aggtaggagg attttatgtt tttcaaagat attgaactaa attcaaaaaa
       61 agagttagac ccatattttg atttagtaga ttatgaagct tgtgagtact gttttagtac
      121 tttatatatg tggcaacatg tttataagac aggatattat ataggtgaag attttgccgt
      181 tttagttggg gaatacgaag gagatagttt ctcaatatta ccacttgcta aaaaagataa
      241 acttccagaa gttgtagatt ttgtccttga atatttttct aaaaataata aaaaaatata
      301 cttaagaggt attactactg aagtagtaga gtttttaaaa gaaaaatatc caggtagatt
      361 tgagtatata gaagaaagag atttatttga ctatatttat gatgcagaaa gtttaagaac
      421 tctagctggt aaaaagaatc aaaagaagag aaaccatata aactatttct taaaagagta
      481 tgcaggtaga tatgaagcta aattattaga taaagaaaac tttgacgagt gtttagtact
      541 aatgaaggaa tgggaaagta ataaggaaga aaataatgaa tttgatgaaa gtatggatga
      601 cgaattaata ggtatcaaaa agatatttaa ccattatgac atattaaaag ataaggtaaa
      661 agtgtttggt gtttatgtgg atggaaaatt agaggctttt agtataggag aacttttaaa
      721 tccaaatatg gcattgattc atatagaaaa agctaatccc gatataagag gtctgtatcc
      781 atttataaat caacaatttt tggtaagtga atttaaggac gttgagtttg taaatagaga
      841 agaagattta ggtattgaag gattaagaaa agctaaactt tcttatcatc cttgcagatt
      901 tgttgaaaag tacagtgtta gggaggctta atagactata tggagattag gtatgctaaa
      961 gaagaagaga tagaaagtat aaaagagatt tggagttatt gttttaacga tacggagagt
     1021 tttatgaagt actattttaa tgataaatat aaatctgaaa atactgtagt agctttagat
     1081 gaaggaaaaa ttatttcttc tcttcagcta aatcagtata agttattact aaatagcaag
     1141 gtatataata catcatatgt agtaggagtt tctacacttc cagagggaag aggagctgga
     1201 tatatgaaca aagtaatgaa atttacttta aacgaattgt ataaaaaagg gcaattagtt
     1261 tctatattga tgcctattga ctatagactt tatagaagat ttggatatga gcattgttac
     1321 gaccagattg aatatactat taacacagat gatttaaaaa attttaagtc aagtggaaaa
     1381 atgataaagt ctaatttatc acaaattgat gatttgatac gaatagatag aactttttta
     1441 aatgaggtta atggtaatgt gttaaaagat gaacattatt atgaaaactt atttaaagag
     1501 attcaaagtg aagatggatt tttatacatt catgaaggaa atgaaaaaga tggatatata
     1561 gtttattttc ttcaggaaga taaaatgttt gtcagagagt tattttataa aaatatagat
     1621 gcccttaaat caatgcttaa atttatatat aatcacaata ctcaatgtaa gatagttact
     1681 atatctactc ctacaataga taagataaga tttatattag acaaccctaa agatagtgat
     1741 ataaaaataa aaccttttat gatgggaaga gttataaatg taaagaagtt tattgaagat
     1801 atagatattg agaaggatat taatagctca tttaatttat taatagaaga caagtttata
     1861 gatgaaaata atggtctatt taaaatttct atacagaata agaaggtatc tgtagagcag
     1921 ttagataaaa aaggtgcaga aaagcctcaa gaagattttg atattaaatt agatataaat
     1981 accttaacac agttatcatt ttcttatata gatgtaaatg aagctatttt cttaaatgat
     2041 ataaaagatg tttcagaaga aacactagaa acacttaatt gtattttttc taaaaaaaat
     2101 acctatataa atgaatatat ataggtagaa tacgttaaaa gattttaaaa atcttttgac
     2161 gtattttttt gtttagataa caatttatct tgtttaaaaa ttttctattt gaaaataatc
     2221 taaataatat acttttatat gggagtgatt ataattcaaa taagaagacc attattaatt
     2281 atattaatgt ttgtggtagc tatatcattt atcattacaa ataacacaaa aaacgacgaa
     2341 gaacttaatg ataagataat aactatacaa ggggttgtca aaggaaaaat tgaaaagaaa
     2401 agatacaatc agtacaaggt aggggttttt ctaataaatg attatacaaa aaataaaaat
     2461 ttgaagattg gtcagaaagt taatattaca ggtaaattca aaagtttaga taaaatgaag
     2521 tatgatgatt ttgattatgg aaggtatata aaaagtactg gatataaagg tatagtgtat
     2581 ataaataact ataaaattat tgataaaaat aaactttact ctttaatagg ggaaataaag
     2641 ttttatatta gtaaaactta taggtactta tataagaaaa actcagattt tataaattct
     2701 atactattag cagaagttga aaatttgact gaggaacaaa aagaaatttt tactagaact
     2761 ggaactagtc atgtaatatc tatatcaggt cttcatacag gaatattatg tgtaattata
     2821 tcctttctat taagaggtat aaataaattg tataaattat taatactgtc gatttttata
     2881 actttatatt gtataatggt aggtgcgtct ccatccataa ttaggtctat agcatttgtt
     2941 atgatttttt atttatcaat atttattgat agaaagaaag atggaatatc agcattgtct
     3001 ctaattggga taattttaat tatgaataac ccctatgtta tatataatgt aagttttcaa
     3061 ttgtcttttt tagctacact ttctatatta atattctata ataaaataaa tagtattata
     3121 aaattaagta tggtatcatt gacaatatca tcaaatatat taactttacc tataatttat
     3181 tatacattta aaggtatacc attgctttca attataggaa atttaattat agtaccattt
     3241 gtgggagtaa taatgtattt aagtattgca agtttaatcg tatttaaagt aagtgtagta
     3301 atagctaaaa ttatatcttt ttttaatagt acattgatag aaagcatatt ttttttacta
     3361 gaaaaaatca gcaatttaag ttttgcatac attaatatag aaagtcctaa attttatatt
     3421 gttgttattt attacatagg agtgtttttt tacatatttt acatagaagg aaaagagata
     3481 aaggagcaag agaatgaatt acaaggatat tataaggaat gtaaaagaga aaaactttga
     3541 aaaaatgtat cttttttatg gaagagagta ttatttaatt gagaatgcaa taaaagcatt
     3601 taaagatagt ctaaacgaag gaatgcttga ttttaattta gatataatag atgggaagga
     3661 aatagtctta aatcagctta taagctctat agaaacactt ccatttatgg atgatagaaa
     3721 gatagtaata ataaaagact tcgaactatt aaaaggaaag aaaaagaatt ttacagatag
     3781 tgatgagaaa tacttaatag agcatttaga taatattcca gacactacaa ctatagtttt
     3841 tgttgtatat ggagatgtag acaaaagaaa atcattagtt aagaaaatag gaaacaatgg
     3901 aattgttttt gattgtgata agctttctga tatggattta tttaaatgga taaaaaaatc
     3961 ttttgcttta aatgatgtta taatagataa ttctcaaatc atgtatttta ttgaacaaga
     4021 aggatacaga gataagagca gtgaaaagac attatctgat ttagaaaatg aaataaataa
     4081 aataagttcc tttgttggta aagggaataa tgtaaccaat gacgtaattg ataaattatc
     4141 tcaaaaaaaa gttgagaatg atatatttaa attaatcgat tatattggag aacaaaatgc
     4201 ttctaatgca atgaaaatat taaatgatat gatacaagaa ggcgaatctg tattaggtat
     4261 attttctatg atagcaagac aatttaaaat aattatgcaa gtaagacagt tacaacttga
     4321 tggatattca acaaaactta tagctgataa attaaaaatg catcaatttg ttgtaggaaa
     4381 agcattaaag caaactaaaa atttttctga tgatataatt gtggaaatat taaactatat
     4441 attagaaagt gattataaaa taaaaacagg tcttataaga gatactttag cagttgaaat
     4501 gttggttagt agatactgta aaagagaagc aatttaaaaa atctatatat taataaaaaa
     4561 taaatagcaa gatatgttat agttcagagt agatgtaagc taaattaagt ttgatataca
     4621 atcaactata atatgtctaa ctatttattt tttattgtat aaatttacaa aataaaaaac
     4681 ccctacatag gggttattat atataagaaa tataaactgg taattaagcg ttcataccgt
     4741 ttaatttttg agctaattta gctacttttc tagctgcagc attcttatgt atagttcctt
     4801 tagaagctac ttggtatatt cttttttgag cgtattggaa ctttgcaaca gcatcctctc
     4861 tgtttccagc tgcaacagca tcttcgaatc ttcttattgc agtttttatt tgagattttc
     4921 taactctatt taaagcagtt ttcttttcga taacacttat tcttttctta gctgatttta
     4981 tatttgccac cgtgttcacc tccctgtatt tttttatcat attcatcgtc aacatattag
     5041 attttaacag aaataaatct aaaaatcaag gattattatt tatatatttt aataatgttt
     5101 tttattttga ttttttagac tgtaaattgt gtataaataa tttgcaatat cttgttttta
     5161 aattggtata ttttgtgttt taaatatttt gacatatata aattaatatt ttatttttgt
     5221 aacaaatatt ttgagataat acaaactatt aacatacatt ttgatagtat ttccaataat
     5281 tataagaaaa tacaagaaat tactcatttt tgtaaattta atgaaaaatt ataaaaatat
     5341 aaatttatat gcttaattaa gataattttt ccaataatca agataatttt tttcattagt
     5401 atgtatgata gatgcatcaa acaatataac taatatgtta gaaggttata tgtaattaat
     5461 ctcagtgttt atatagaata gcatttgtag catttattat agttgtaatt actacttata
     5521 tcatagactt gaatagtcta aaaaaataga ctataatatt tttatagact aaagaaaggg
     5581 ctgataagat ggacatagca aaactaattt taaatcctgc aaggcttcgt atcttacaat
     5641 atattcgact tcatggcagt gctcgcacat ctgatattgt aaagtattta aatgatattc
     5701 ctcgtgcaac agtttatcac catgtaaaaa ttttagaaga gaacaatatg attgaagtaa
     5761 ttaaagaaaa tcgtgtgcgt ggtactattg aaaaggttta tgcactaaag gaatatacaa
     5821 catctatgga aggagagacc ttcgttgcac tttctacagc attttatgta ggtctgatgc
     5881 aggaaatgaa tgaatacttt agcagaaaag agcaagacca taaaaaggat aatgtatttt
     5941 tctcttcagc attactatat atgagtgata atgaatatga aaatcttctt aaaagtatag
     6001 ctgatttatt gaaaccatat atagaacaaa agcctaaatc aaatctaaaa cttagaaaac
     6061 tttcaattat atcttctcca cctgtggttg ataatgataa aacaaaataa catgaatctt
     6121 atttttataa aaggaggttg tatatgaaat ttcatgaatt tggagataaa aacaaaccac
     6181 atattatgtt gattcatggg ggagggaatg cgtggtggaa ctatttgcga caagcagaag
     6241 ttttgtctaa caactatcat gtgatacttc ctacattgga tggtcatgga gaagaatatg
     6301 aaacaacata catttctaca gaggatacag cagataagtt gatatcatat atagatgaaa
     6361 attgcagtgg acatcttttc gctttgtgtg gggtatcact tggtggtcaa attgtcatgg
     6421 aacttttatc tcgaaaacct aatattacta aaaaagcaat tattgatggt agtatttgct
     6481 acccaagacc tcttatggca aggttttgta ttgctggtat tcgatttttt ggaggacttt
     6541 tgtttagtga gaaggcttgt cgctttcaaa ttgctatgat gccaaaattg ttacctgaaa
     6601 atatgcaata tccagatgaa ataaaagcgt attatatgac agatatgcca catgtaagaa
     6661 aagaaacatt ctataatatg tatcgtactt atatgatgaa ctatacactt aaagagaatg
     6721 ttaaaaaaac cacagcacaa gtaatgtact ggtatggaga aaaagaaatg aagtgcgtta
     6781 aaaaatctgc aaaaatgttt cagtcttatg taccctcttg tgagatttat gaagctaagg
     6841 gctataatca tgggtattta tcgctttatc ttccaaatga atggttggaa attgcagaac
     6901 catttttcca aaaggaataa aggtgttagg tgattattgt ataattattg ctgttttttt
     6961 atatagtaaa taaattaggt ataaatataa aaaagttatt ttagttaata tattagtgaa
     7021 agcttaatag gaaaattata aatagattaa aggtagcgcc tatataaata gttgctacct
     7081 ttttttgctt gtaaataaat gatagataag aaagtttact gacaaatcac tgactaaacc
     7141 caattttctt acattgaaag ccttaatttt acttgtttta aataaattta caatttcttg
     7201 ttataaaata ctaaattttg ttaacaatta taattagcat ttaatttata ctggaggttt
     7261 atatgattaa tgtaagaaca gatttagctt tagaagctag tgaaatgtgt gaaaagtctc
     7321 aagaaggaag tagtattcct ggtgtaaaaa tagaaactaa agaattagaa aattgcattg
     7381 taactaaagt tgaagtaata gacaggcaag gcgcagagat aatgaataaa gatataggaa
     7441 agtatataac tctggaaagt aatcttatga agtttgatga tgatgagtcg agagaagaaa
     7501 tgataagtta tttaaaagag gagttagtag atatatttgg ccaagacaaa aataaaaaaa
     7561 ccctggtaat tggtcttgga aatagaaata ttacttctga tgcgttagga ccaaagagtg
     7621 tatcaaaaac acttgtaact agacatcttt ttaaaaatta taataaagac tatgatgatg
     7681 atttcactga ggtatcagct ttaagcccag gagttatggg tgttactgga atagaaacta
     7741 gtgaaatagt aaagtcattg gttgaaaaag taaaaccaga tagagtagta gcaatagacg
     7801 cattggcttc aagaaaaatg gaaagagtaa actctactat acagatatca acagcaggaa
     7861 tatcacctgg tggaggtgtt ggcaatacta gaaaatctct aactaaagaa actttaggcg
     7921 tggatgttat tgcaataggc gttccaacgg tggttgatgc agcaactctt acaatagatg
     7981 tcttagatat ggcaatagac aatttaatag ctcaatcaga agaaacagaa agtttttatg
     8041 aaatgttgaa aaaacttaaa gaagaagaaa aatatcatct aataaaagat tcgttagacc
     8101 catatgacaa gaacttaata gtaacaccaa aggatataga tgatacaatc gaaaatctat
     8161 ctataataat aagtgaaggc ttaaataggt ctttacatcc aggtagattg gtataaaata
     8221 aggaggagca attatgttta aaaaatgcat taaagtagta accttgactt ttatattagc
     8281 atgcatttta ccaggaaaat cattagcttt gaatcaggat gattttttaa agtttttagt
     8341 aaattcatct tatccagaag ctaaagtcga aggaaatgat acagaaaata aaaagaataa
     8401 taaaaataaa gaaacaagca aagaaaataa ggaagaaagt aaagaagaaa acactaaatc
     8461 taaagatgca agtaaagttg ataataaaaa agagagtgaa aaagaatata taaaacttta
     8521 tgttggtaaa gaaaatgttc cagatattga gtcaaaaaat tcagacacaa ctgaaactaa
     8581 cacaacatct agttcagatt ataaggatga tttaagagta accaaagaaa atccaagaat
     8641 tttgatatat catactcatg gatgtgagac ttattcaaat tcacctgatg gaaattatca
     8701 ttcaagagat aagaaaaatt cagtaatgga agtaggaagt gcattaacta gtgctttaga
     8761 tagcaaagga tggggtgtag tccatactac taaataccat gactatccat cttataataa
     8821 ctcttatgca agtagcttaa aaacaataca aagtatatta cctaaatata actcagtgga
     8881 tatagcgata gacttacata gagatgctag ggatttaact aatccagcca ctaaggaaaa
     8941 agaccattta aaatatacaa ctatgataaa tggagagaga gtatcaaagt tcttctttgt
     9001 tgtaggaggt aaaaatacaa atagaaaaca acttagagct ttagcagaag atataactgc
     9061 ttttgctgag aaaaagtatc ctggtctagt atctccaata gtagaaaaag actatgctag
     9121 attcaatcag tttgcagtta agaatcatat gttagtagaa atagggaata acgcaacaag
     9181 tgtagaagaa tctaaggcta ctacaaagta tttagccgag attttagata aatattttaa
     9241 acaaaaaaat tagagcttta agttataatt aggagcatgc taaatgagcc gattagaaaa
     9301 aaatatagaa agtaaaagta aactaagaaa atacaagtat attatgagaa tcttattttt
     9361 gattactatg attataggta catcagtttg tgtatttata gtagacagta atgcaaaaag
     9421 tatgctagga atgaaacaat atgattttcc aagtattaaa tttgatataa caaaatttga
     9481 ttttgacata ccaaattttg atatacatga aatcataaaa aaatttaata agtagtaata
     9541 agtgaataag gtttattgat taaaataagc cttattttag ttatatattt ttaatgtata
     9601 aaataaaaat tcattaactg tattttatag gatttacttt aagtttacga tattatataa
     9661 tatttagtgt tagacttgat aatccacata taatgtggat aattttgttt agtgccaatt
     9721 agtttatatt caaaaatttt aaatttggta tataaagagt ttttagataa tgtaaaataa
     9781 taactataag aataatcaag aggagggaat tttaaggtgg acaataaaca aagtagaact
     9841 agaaatttta gtataattgc acatatagac catggaaagt ctaccttagc tgatagatta
     9901 atacaacaaa ctgggcttgt tagtgaaaga gacatgaaaa gtcaattact tgacaatatg
     9961 gaccttgaaa gagaaagagg aatcactata aagcttcaaa acataagatt aatgtataaa
    10021 gcgaaggatg gaaatgaata ttatttaaat cttatagata cacctggaca tgtagacttt
    10081 aactatgagg tatcaagaag tttagctgca tgtgaaggag cactattagt agttgatgca
    10141 gctcaaggtg ttgaggcaca aactttagcc aatgtttatc ttgctataga ccaagattta
    10201 gaaatattac caataataaa taaaatagat ttaccaagtg caagaccaga ggaagtaaaa
    10261 aatgaaatag aagatttaat agggcttgat tcaagtgaag ctcctcttat ttcagctaaa
    10321 actggtttaa acatagaaga tgttttagaa gatattgtaa aaaatgttcc tccacctaag
    10381 ggagataatg aagctccatt aaaggcatta atattcgatt cttactatga tgcttacaaa
    10441 ggtgttgtag cttatgttag agtatttgaa ggaactgtta aaaagggtat gacaataaaa
    10501 atgatgaata ccaataaaaa gtttgaggta actgaagttg gtgttatggc tccaggtcaa
    10561 actgaactta gtgaattatc agcaggtgat gttggttata ttgctgctag tataaaagat
    10621 ataagaagtt gtcgtgttgg tgatacaata acagattcaa ataatcctac agaagaacct
    10681 ttaccaggat acaaaaaagc tactccaatg gtatattgtg gtatatatcc aggagaagga
    10741 gaaaagtatg agaatgttag agatgctctt gaaaagcttc aagtaaatga tgctgcactt
    10801 gaatatgaag ctgagacttc agcagcacta ggatttggtt ttagatgtgg tttcttaggt
    10861 cttttacata tggagataat gcaagaaaga cttgagagag aatttaacct tgatataatt
    10921 actacagctc catctgttat atatagagtt acaaaaatgg atggagaggt tgtaatgata
    10981 caaaatcctg caaacttacc agaaccatct gagataaaga tgatagaaga gcctatagta
    11041 aaaggagata taatagttcc taaagactat gtgggtgttg ttatggagct ttgccaggaa
    11101 agacgtggaa atatgttaaa tatggaatat atagatgaga gaagagtaat gcttcactat
    11161 gaccttcctc tcaatgaagt tgtttacgat ttctttgatg ctttaaaatc gagaactaga
    11221 ggttatggtt ctcttgatta tgaggttaaa ggttatgtag catctacact tgtaaaactt
    11281 gatatattaa tcaataaaga acaagtggat gcacttagtt ttattgtcca tgagacacgt
    11341 gctttcccta gaggaaaggc aatgtgtgag aaattaaaag gagaaattcc aagacatcag
    11401 tttgctatac ctatacaagc tgctgttggt aataaggtta ttgcaagaga aacaataagc
    11461 gctctaagaa aagatgttct tgctaaatgt tatggtgggg atatatctcg taagaagaaa
    11521 cttcttgaaa agcaaaaaga aggtaagaag cgtatgagac aaattggttc tgttgaagtt
    11581 cctcaaaaag catttatgtc tgtacttaaa ctagatgaat aaattcttat aaatttagat
    11641 aaatatattt aaaagctaga gtgattttag aattatttct aaattaatct agcttttttg
    11701 tggtatattt taagtaaact catagagaat ataagtaaga atatattaaa gaagaataaa
    11761 aaggtgatat ttatgattaa tatagaaatt cctaaaaaag tagactatat aattaaagaa
    11821 ttagaaacga atggttatga agcttatgta gttggaggat gcgttagaga ttgtttatta
    11881 gaaagagttc caaacgattg ggatataaca actagtgcta gaccagaagt ggtagtagaa
    11941 ttatttgaaa aaacaattcc aactggtata caacatggga ctgtaacagt tatgattgaa
    12001 catgaaccat ttgaagtgac aacttataga attgatggaa attatagtga tggtagacat
    12061 ccagattcaa tagaatttac aaataacata gttaaggact tgtcaagaag agattttact
    12121 atcaattcaa ttgcatacaa ttcaaagaca ggattagtcg acccatttaa tggctatgaa
    12181 gatatacaaa ataagtatat acgttgtgtt ggaaatccag tagatagatt tgaagaagat
    12241 gcacttagaa tgcttagggc agttagattt tcagcacaac ttaattttaa aatagctgaa
    12301 ggtactaagc aaagtattca taaaaaagca gatttgataa aaaatgtttc tatcgaaaga
    12361 atccagacag agtttaataa aatattagta tctgattcat ctaaattaaa cttattaaaa
    12421 tcaacaggtt tgttgaaatt tataattcct gaaatatgtg aattagaaga tgttactcaa
    12481 cacaatccat atcacatcta tgatgtacaa aaacatactt taatatctac agaagtaatt
    12541 gaagatgaac tgtatttaaa acttacaatg ttgtttcatg atttaggtaa aaaagtaact
    12601 aaaacaactg ataaaaatgg tgttgaccat ttttatactc atagtagaga atctgtaaag
    12661 attgcaaaga agattttgag aagacttaag tatgataatt atactattaa taaagtttta
    12721 atattaatac aatatcatga ttatagaata gaacctaaaa ggaagataat taaaaagtta
    12781 ttaaataaac ttgaagatgt tgaattattt gaagatttaa taaaagttaa ttgggctgat
    12841 acacttgcca aaaacccaaa gtatgcaaaa caaaagatat taaatcttat tgagtgtgaa
    12901 aaagaattta aacatataat aaatcaaaag gagtgcttta atcttaaaga tttagctatt
    12961 aatggaaaag atttgatatc tataggtata aaaccaggta aagatatagg acatatttta
    13021 aataagatgc ttgaaatagt cataaataat ccagaattaa atgaaaaaga aatactaaaa
    13081 gaaaaagcat taaatatata tacattttag attcaaatat aaaaaattta attactttaa
    13141 aaaattatag aagaaaatac ttatttagat aaaataaata atttttagag aatattttag
    13201 aagttacgat tgtatgattt ttaaacgcat agcatttact ctaggactat agactaatat
    13261 aaaaaaaaac tttgggaata ctaaaaccat aaatttataa aattaatatt ttatatactg
    13321 taaaacagtg aggagggaca gtatgcatac tcaactacaa aagactattg gtctttcagc
    13381 ggctctttct actgttgtag gtatggttat aggttcggga gtttttttta agccacaagc
    13441 aatatacact acaactaatg gagctccagg tctaggtatt atagcgtggc ttttaggtgg
    13501 ttttataaca ataactgctg ggcttacagc tacagaaatt tcagctgcta taccaaaaac
    13561 tggtggtatg atgatttata tcgaagaaat atatggagaa aagttaggct ttttaactgg
    13621 atggatgcaa actgtattat ttttcccagg tacatcagca gcattaggag taatatttgc
    13681 tcaacaagca tctgaacttt taggaatgag cccaaataat atggcaaatg tacttcctat
    13741 agcaattgga gttatactat ttttagcgtt attaaatata ataggttcat ccttaggagg
    13801 aaaagttcag acagtagcca ctattggaaa gatgatacct ttaattttaa taatagtctt
    13861 tggatttatt aaaggtcaaa gtagtgaagt attaaatcca tttgtaggag atggtgtaaa
    13921 tgcatcaaat gcacttggac aagtattaat agctactttg tttgcatatg atggttggat
    13981 aaatgttgga gctatatcag gagaaatgaa gtctccagag aaagatttac caagagcaat
    14041 agttggtggt ttatcattag ttatggcagt ttatattata ataaatatag cttatctttg
    14101 ggtagtacca gcaagtgaat tagcaacagt tacttcccca gctacattag tagcaacaag
    14161 attgtttgga aatataggtg gaaaagtaat aactgtgggt atattaatat cagtatttgg
    14221 gacattgaat ggatatttat taacaggttc aaggatacct tatacattag ctgaaatggg
    14281 aactttacca gcatctaaaa ctcttttaaa agtaaattct ggaggttctc ctgtaaattc
    14341 aatactactt ataactgtgt tagcctgtgt atatgcatta tctggtcaat ttaatctttt
    14401 gacagactta actatatttt caatatgggt attttatgta ttgactttta taggtgttat
    14461 gaggcttaga agacaaaagc cagatttaca cagaccatat aaagtccctt tatatccaat
    14521 aataccaatt atagctatac taggtggact gtttgtaata ataaatcaaa tattgacttc
    14581 aacaattata tctttaggtg gaatatttat aacactatta ggtttaccag tttattatta
    14641 tatgaaaaaa agataactac ttaatttaag ttagataaat ttgggtaaag gtgaaaatta
    14701 acttttatcc aaatttttta tactgttata caatataagc attatatatt acttgatgtg
    14761 ttattttgtt tgatttataa tttagtaatt gatgctttaa gataatttaa gaaatgagga
    14821 ataaaaaatg ctaggacttt atgtacatat tccattttgt gtgaaaaaat gtaagtattg
    14881 tgattttaat tcttataaaa tggacataga ttcaaaaaaa agatatatag aagacttaaa
    14941 aatagaaatg aaattatata gtaataaatt atataaagat aataaatata aaaataaaga
    15001 atgctgtagt ctaaataaaa atgacaaaat aacgagtata tttgtagggg gaggtacacc
    15061 tagtatacta acatctgatg aaataagaga agtatttata agtataaaag aaatgtttga
    15121 tatagatgag aatgcagaaa taactataga gtgtaatcct gggacattaa cattagaaaa
    15181 attaaaaact atgaaagaga ttggcataaa tagacttagt atagggctac aggccattca
    15241 agaaaagcat ttgaatttta taggaagaat acatacttat gaagaatttg agaaaaacta
    15301 taaagatgca ttaagtgtag gttttaaaaa tattaatgta gatttaatgt acagtcttcc
    15361 aaatcaaact ctatgtgact ggaaggaaac tcttgaaaaa gttgtacact taaatccaac
    15421 acatatatca gcgtattccc ttatactaga agaaggaact gaactatata atatgtatga
    15481 aagtaataaa tttgagctta tagatgaaaa tgtagatata gaaatgtatg agtatacaat
    15541 aaactactta aagtcaaaag gatataatca atatgaaata tcaaattact caaaagaagg
    15601 ctataattgt gagcataata ttttatattg ggaatgtgaa cattatatag ggattggagc
    15661 aggagcttct ggctatatta atgagaacag atacaataat gtagaatctt tagaagatta
    15721 tcatttaagt ttggtaaaaa gagaaaaacc aatacaagaa aatgaaattc tttctgagaa
    15781 agatatgata gaagaaaaga tatttatggg gcttagaatg aataaaggca taaaatttga
    15841 agattttaaa aaaaaatttg gaatagattt tagagagaag tataataagc aaatagaaat
    15901 gctattagct agaaagctta taaatcagag ctttgaaggt atacaattaa ctcaaaaagg
    15961 aagagaaata tctaatagtg tatttataga atttatggaa taatttttaa ggtgttgaca
    16021 aaataaattt ataatggtat ataatatata taatcttttt agcactcgaa cttagtgagt
    16081 gctaacagct aggaggtata ataatatgga attaaatgaa agaaaattaa atatcctcaa
    16141 agctatagtt aaagattata tagaaacagc tgaagctata ggttctagaa caatatctaa
    16201 aagacatgat ttaggtgtta gtgctgctac cataagaaat gaaatggctg accttgaaga
    16261 attagggtat ttaattcagc ctcatacttc tgcgggtaga gttccatctg aaaaaggcta
    16321 taagttatat gtaaactctc ttatgagtaa aagcgaattg gatgataatg ataagattct
    16381 tattgaacaa tgtatgaatc ataatataaa tcatataaaa gaattgattc atgaaacctc
    16441 taagctatta tcgcaactaa caaactatac aactgtagca gttactaaga gtttaataaa
    16501 tcaaagtgtt ataaagcaca tacagttggt tgctatgaat gacaataata ttttacttat
    16561 agttgtaaca gataaagggg acttaaaaaa agctaacctt acaactaatg tatatttaga
    16621 tcaatctaag ttaaatttga tttctgataa tcttacaagg aagcttttag gtaaaagtat
    16681 aacagatttg gatgataatc taattgcttt tattaaatac gaaataagtg agtattctgg
    16741 acttatagat gagcttttaa atgctttgaa ctcaaatatg aaagaagaag atttttctct
    16801 atcattaaat ggagctacta acatatttag ttatccagaa tttaatgatg tattaaaagc
    16861 aaagtcattt ttaaatatgt tggaaaaaaa ggaaacaata gctgacataa ttaaatcaaa
    16921 gggaatacaa aaagacaatc taaatataat aattggtagt gataatgatt gtgaactggc
    16981 acaggattgt agtatagtta cagcaactta taatgtagat agagacttgg ttgggcgaat
    17041 aagctttata ggccctacca gaatggatta tgctagaatt tactctataa taaattatat
    17101 gagtttatta attaatagaa agtaaagaaa ttttaaattg cgaggtgtaa agtcttggat
    17161 aaaaaaaatg agcaacagga agtaagagaa gaaaatgata cttctattaa ccaagaatcg
    17221 gaaactcaag tagagttaga agaggaagtt gttaatgaag aatgtgaaac atcaagtgaa
    17281 aaaactgatg aaaaagaagt tgatgatgaa aatgtaacag atataaattc taagttagca
    17341 gaaaaaaaat tgcaggatga gttggatgaa ttaaatgata aatatcaaag acttcaagcc
    17401 gaatatgcaa actacagaag aagaactcaa caagagaaag agacaatagg cgtatttgca
    17461 aatgaaaaaa taataactga gttgatacca gttatagata gtatggaaag agccttagat
    17521 gcatgtgaag ataaggaaga tactatgtat aagggtataa gccttgtaca taaacaatta
    17581 atagacactt tggtaaaatt tggagtagaa gaaatagaag cagaatctaa ggaatttgac
    17641 cctaatctac atctagcagt tatgcaagaa agtgtagatg gagttgaagc aaatcaaata
    17701 gtaatggtac ttcaaaaagg atataagcta ggaactaaag ttgtaagacc ttcaatggtt
    17761 aaagtttctt gctaagtata aattaaaggt taaattattt aacctaatat aaaatattaa
    17821 aataagtaaa acttaattat ataattactc gaggaggatt ttagtcatgg gaaaaataat
    17881 aggaatagat ttaggaacaa caaattcatg tgttgcagtg ttagaaggtg gagaagcaca
    17941 aataatagca aatagtgaag gtatgagaac tactccatca gtagtagcat ttacaaaaga
    18001 tggtgagaga atagttggag aacctgcaaa aagacaagca gttacaaatg cagataaaac
    18061 aataacttct ataaaaactc atatgggaac tgattataaa gtaaatatag atggaaaatc
    18121 atatactcca caagaaatat cagcaataat attacaaaaa ttaaaatcag atgcagaaag
    18181 ttacttagga caaacagtaa cagaagcagt tataacagta ccagcttact ttacagatgc
    18241 tcaaagacaa gcgactaaag atgctggtag aatagcaggg cttgatgtta agagaataat
    18301 aaatgagcca acagcagcag ctcttgctta tggtatggat aaattagacc aagaaaagaa
    18361 aatattagta tttgacttag gtggaggaac ttttgacgta tctatacttg aaataggaga
    18421 tggaactttt gaagttttag ctacagctgg taacaataga ttaggtggag atgactttga
    18481 ccaaatagta atagattatt tagcagatga atttaaaaaa gctgaaggtg tagatttaag
    18541 aaatgataaa atggctcttc aaagattaaa agaagcagca gaaaaggcta agaaagaatt
    18601 atcatcaaca atgagttcaa acataaactt acctttcata actgctacag cagaaggacc
    18661 aaaacactta aatatagatt tatcaagagc taaatttgaa gaattaacta gaggtttagt
    18721 tgaaaaaact atggaaccaa ctaagacagc tttacaagat gcaggacttt ctacaggtga
    18781 tatagatgat gtattattag taggtggttc tacaagaata ccagcagtac aagaagctgt
    18841 taagaagttt ataggaaaag aacctcataa aggtataaac ccagatgagt gtgttgcagc
    18901 aggtgcttct atacaagctg gagttttagc aggggatgtt aaagatttat tattactaga
    18961 tgttactcca ttatctcttg gtatagaaac tatgggtaat gtaatgacta agataataga
    19021 aagaaatact acaataccaa ctaagaagtc tcaaatattc tcaactgctg ctgataacca
    19081 aacagctgtt gatatacatg ttcttcaagg tgaaagaagt atggcttatg acaatacaac
    19141 tttaggaaga ttccaattaa cagatatacc accagcacaa agaggaatac ctcaaataga
    19201 agttactttt gatatagatg caaatggtat agttaatgtt tcagctaaag atttaggtac
    19261 aggaaaagag caaaaaataa ctataacatc aaacactaat ttatcagaag cagaaataga
    19321 gcaaaagata aaagaagcag aaatgaatgc agaagctgac aaacaaaaga aagaaaaaat
    19381 agaagcattc aatcaagctg aatctactat atatcaaact gaaaaaactt taaatgaact
    19441 tggagataaa ataagttcag gtgaaaaaga ggatatagaa aaagctatag cagacttaaa
    19501 agctgtaaaa gataatcaag atgctacagc agaagaactt aagaaagcta cagatgaagt
    19561 aatgactaag ttccaaaaag tatctcaaga aatgtaccaa aaagcagctc aagaacaaca
    19621 agcggcacaa ggtgcagaac aagcacaaga caatggacct aaagatgaca atgtagtaga
    19681 tgctgacttt aaagaagtag atgaagataa ataagctata taatatgtaa cactaaatga
    19741 taattggaac atatatgatt tgtgaaaatc atatagatgt taaagatata tggacaatac
    19801 acatcataaa gaatataata tatcatagat tatataactt aaagtagaat ataagaatta
    19861 atgatagctt gtagtttatt attctacaag ctatcgtttt gtaaaatgaa ggtggtgtca
    19921 atttttgagt acaaaaaggg attattatga agtcttaggt atcagtaaag atgcagaagc
    19981 tcaagagata aaaaaagctt atagaaaatt agcaatgaag taccatccag atagaaatcc
    20041 tggagataaa gaggcggaag aaaaatttaa agagataaat gaagcttatg aagttctatc
    20101 tgatgataca aagagaaaaa cttatgacca gtttggacat gatggattaa atggtcaagg
    20161 tggttttggt ggtcaaggag gatttggcgg tcaaggcttt ggtggttttg aagatatgtt
    20221 cggagatata tttggtgaca tgtttggagg aagcttcgga ggtggaagag caagaagaag
    20281 agggcctcaa cgtggtgcag atataagaca gagtgttact atatcttttg aagaagctgc
    20341 atttggtaaa aagatgtcta taaaagtaaa tagaagtgaa gagtgtgaag aatgtaatgg
    20401 aactggagca aaaccaggaa catcaaagaa aacttgttca acttgtaatg gaactggcca
    20461 agttagaact gttcaaagaa ctccatttgg taatatagca agttcaagac catgtagtgc
    20521 ctgtaatgga actggagaag tgattgaatc tccatgtagt aaatgtcatg gaactggaaa
    20581 tacaagaaaa gttaagacta tagaagtaga tatacctgct ggtatagatg atggtcaaat
    20641 gataaaactt tctggtcaag gtgaagtagg agaaaaaggt gctccaagag gagacttata
    20701 tattgtagtt aatgttaagt cacatccact atttactaga gatggaaatg atatttattt
    20761 tgaaatgcca attacatttg ttcaagcaac tttaggtgat gagatagaag tacctacttt
    20821 ggatggaaaa gttaaatata gtgtgccaga ggggacacaa actggaactg tatttagact
    20881 taaagagaaa ggaatcccta gaattagagg taattcaaga ggagaccaat atgttaaggt
    20941 tgtagttgaa attcctaaaa aattaaacga taagcaaaaa gaactattga gagaatttgc
    21001 gaaggagtgt gggtcgaacg tccacgaaaa gaagaaaacc ttcggacaaa agatagaaga
    21061 tatgtttaag aaaaagtagt taaagtagcc aatatataat catatttttg aaatcttagt
    21121 tatttataag attgtcaaag gagatattat gatttttata ttggctattt ttgtataaaa
    21181 aataaatgtg cataaaataa aactgaatat aaataatttt ctgaaaaata cttgtcctaa
    21241 aatatataat gtgctataat ataatcaaat aaaagaaagg ggtgattcca gtgggaacgg
    21301 atgtggcact gccaaatgat gaagaaaact ttattgtaat atacaaaata aaattagata
    21361 cttttagtgc tagattaagt actggaaaaa ctcatgagct gggataatta ttgtaattac
    21421 ctagactcaa acttttagta gtaaaatagt aatattatat agacaaggta tctaattttt
    21481 aaatcttttg tatgagttaa aatttttata aagggtatat ctgattagaa tgtaattagg
    21541 gaaacctagt tgtgaattac ttttagtata tacatgtgta aagaaatatg tatatggtat
    21601 tggttattgt gctagatgtg tatgtaaagg aatatgtgtc tagttgggta tatgtaaaga
    21661 aatatactta taatctatga aaaattttta gtataaattg ctaaaacatt gagattgttt
    21721 tttatagaaa gttagtttat accaactaaa aatgtgctta agatttgttt gatgtttaat
    21781 tataaataag gttgtagata aaaaaataag taaagacaaa ggtttaaaat acttgcttta
    21841 aatatagttt gttttgaaca cttgccaaat taaaataatt aataaaatag acatcttctc
    21901 atagtaaaat atactgtgag aggatgtttt tgctttgttc ataaactttt atgaaaaatt
    21961 tgcatagtgt cacattttaa tatactatat atataataaa atatagataa aattaaatga
    22021 ttcaaaagag aaaggtgaaa agagatgtat tatataggtg tagacatagg aggaacaggt
    22081 atacaagcag gagtagttga taactatggg aagataattt ttaggtcaga gtgtaaaaca
    22141 gtaattgaaa aaggctttga aggaatactt aatgacataa aaataatgat ttataagttg
    22201 ttggaagata ataaacttac tatgagtgac ataaaatcta ttggatttgg tgttccaggt
    22261 tttataaata aagaaggatt agtaacttgt gtaaatttaa aatggaataa aaaagctttt
    22321 aataaagagt taaaaagaag atttccagat gctgaaattc atggagaaaa tgatgcaaca
    22381 gtagcagcac ttggagaagc aaagtttgga agtatgaaag gtgctaatgt tggagtatta
    22441 tatactttag gaactggtgt aggtggagga atagtaatta atcaaaaagt cttttctgga
    22501 gcacatggat tgggttcaga aataggtcat caaattatag gagaaaacta ttttaattgt
    22561 aattgtggaa ataatggttg tgtagaaact ttttgttcag caactgcaat tataaagtat
    22621 tctcaaaaac tgatagaaga aggagaaaag agtaggattt tagatttggc agagggaaat
    22681 ttagaaaatg taaatgcaaa gatggtgttt gatgcatata gagaaaatga tttagtggca
    22741 attaaagtaa taaatagatt taaagattat ttagcaaaaa cttttgcaaa cacaataaat
    22801 tctttagacc cagaaattat atctatagga ggaggcattt ctaaatctag cgatataata
    22861 cttgatggaa tagaagattt ggtaagaaaa tttgtattat acaaaacaga agatatagct
    22921 acaattacat gtgcaacttt aggttcagac gcaggaatta taggagcagc ttttttataa
    22981 tgtaaatgaa ctgcttattg gaggcgtgat gatatatggg taaaacaaat gctagaagaa
    23041 cgcttttttt gatagcaata ggttcaggaa ctatgctgaa tcctcttaat tcttctatga
    23101 tttcacttgc tttacatagt atacaaaatg attttcatat tagttttaca actgtatctt
    23161 ggattgtttc agcattttac ctagctagtg ctattgcaca accagtttca ggtaagatag
    23221 gagatttaat aggtagaaag gtcttatttt tgagtggttt gatgcttgta attttatcag
    23281 catccatagt accacttgtt caatcattct ttatattgat ttttataaga gtaattcaag
    23341 caattggaac aagtacccta tatccatctg gagttgctct tatacaaaat aatataaaag
    23401 aaagacaatc ttctgcatta gcagttctaa ccatttttgc ttcaactaca gcaggattag
    23461 gtccgacact tggaggatta ttgttggact taggaggatg gcatgctata tttcttgtaa
    23521 atatacctgt tgttttggtt tctatatgct tagggtattt tttattccca aaagaagtaa
    23581 aagaaaagaa atctataaaa gaaactttaa aaaatgtttt gtcaagatta gatattattg
    23641 gcatacttct attttcaatt gcaatgattt ttatattact atttttgcta tctatgaagg
    23701 aatcttttaa cttagaacaa cttgtatttg gtataatact gatgtgtctt tttatttggc
    23761 atgaattgag aacaaaattg ccatttatag atattaagct ttttgtaagt aatcctaaat
    23821 tgtcaaaagt ttacttgcaa tttattatat taaatttctt taattatatt ttgttttttg
    23881 gtcttcctag ttattttcaa gatgccttac attatagtgc aaagtcgact ggattattta
    23941 tgctatttat gtctggtata ggtatattta tatctccatt aactggaaaa tggatagata
    24001 aatctggaac tagatttcct cttgttacat catcaatctt tatgtttatt agtgcagtac
    24061 ttctttcagt agtttttatc catatacctg taataggaaa gggtattata ttatcattag
    24121 caggtattag ttatggagtt ggtaatgtag ccctgcaatc ctctatgttt gaagaaagtc
    24181 ctcgtgacag tatagggaca gcttctggtc tgtttcaaac tagtcgttat ttaggttcta
    24241 ttttagcaac tgttttactt ggaatggtat ttgaaggaaa tataacatca gaacaatttc
    24301 agatattagg ttatgttatg gtggtcttag gtacaataag ttttttatta aattttatat
    24361 taaagaaaga attaagaaaa gtagaatagt ggatttatgg atttaataag tctaataact
    24421 gataagttta aaaatattgt ttgtaaaaca taactggttt gatatttatt aattctaagt
    24481 tttgaagcat agagtactcg ataagtatac aatattttga attaaaatta agcagaaatt
    24541 tgaactagtc tttcaaatct ctgcttaatt tagtagttaa atttttttat agagaaaata
    24601 agatttgaca cttagataaa aaatcattgc attttcaaaa ttctttaaat caaacccaag
    24661 tatctcatta atcttttcca atctatatac tagcgtgttt ctatgtaaaa acatactagt
    24721 agatgtctta gaaatattta aatcattatt tataaatttt attcctgtat atagaagttc
    24781 ttcgtcaata gatttaatct tattaaaatc tattaattta ttgagtatat tatcctcatt
    24841 aattaaatct aaagttttat atctaataag gtcaaacaaa tttaaaaact tattttcagc
    24901 attgtctttt ataatatgag gtagtaaatc agaacataac tgtaatctaa aataaaaatc
    24961 agctttacta tctaattcac caagataaaa aatataagta ctttgataag tttcagtttc
    25021 aattatattt tgtgtagtta cattaatatc taaattatat ttagaattta ctataaaaaa
    25081 accattgtgt tgtattatat ttatatcttc aaataaatta taaaaaatat tttttaggta
    25141 ttcaaagtct atatgagatt ggtaaattaa ataaaagttg ccttttaaat tatcaagatt
    25201 atttttatct agaaaatcaa taacataaat agcattattg aaagtaacta aatggctact
    25261 agcatctaac ttttggtttt catagggata gatatttatt tttttattaa gctgtctttc
    25321 taaaaaatct tttaactctt ccataaagtc tccttaaaaa taagattata ttcttatctt
    25381 atagctaaag tagtttcttt gtcaaatatg tgtactctac tattgtcaac tgaaagtgtt
    25441 aagtcatcac caacattaat tctgttttta ctgtcaaatc taattgttat cgtattttcg
    25501 cctagtttta agtgagcata agtttctgct cccataagtt cttttatttc aattcttgat
    25561 ttaaaacaag tatctggatt agatttaata aaagtatctt ctacatgaat atcttctggt
    25621 ctgataccta taacaacttc attattaata taattgtctt ttattaaatc atagtgtttt
    25681 tctttattca atttaagctt aatatgttca ttttgtgcat aaattttatc attttcttct
    25741 attaatatta catcaataaa attcatctgt ggtgctccaa taaatcctgc aacaaatata
    25801 ttagctggat aatcgtatac atcttgtgga gttgcaatct gctgaactac tccatccttc
    25861 attactacga ttctatcagc catagtcata gcttcaactt ggtcgtgtgt tacatagata
    25921 aatgtagttc caagctgttg gtgtagctta gttatctctg ttctcatagc agttctaagt
    25981 tttgcatcta agttggatag aggttcatcc attaagaata ccttaggatt tctaactatt
    26041 gcacgtccta aggcaaccct ttgtctttgt cctccagata atgttttagg ttttttattt
    26101 aaaagaggta gtaagtctaa tattttagca gcttctttaa cttttttgtc tatttcatct
    26161 ttaggcaatt ttctaagttt aagtgcaaat gccatgtttt cgtaaacaga taagtgggga
    26221 tataaagcat agttttgaaa aaccatagct atatctctat cttttggttc tatatcattg
    26281 acaagtttat ctccaatgta tagttctcct tcagagattt cttcaagacc agcaatcatt
    26341 ctaagtgtag tcgatttacc acaaccagat ggtcctacta aaacgataaa ttctttatct
    26401 ttaatgtcta tatttatatt ttttactgcg ttaaatccat ttgagtaaag ttttgatata
    26461 ttttttaaaa ttacttctga cataatattc tccttgatat ttaaatttta atgatatagt
    26521 atcaaatttt tttattatat taaatgtgta aaatgactaa atgtatttgt ttaatatgta
    26581 tatttaattt ttagcaaaga ataaaactaa cagtaaaata tatttaaaaa attaaaaaac
    26641 aaaattaaat ttattatcaa aaaatcattt tatgatatgt tttatttttt atattacaaa
    26701 tagagaataa tgaattatac tagaaagtag gaggtgaaaa tatgagcaga atttcaattt
    26761 ttatggagtc atgtaatggc aaatttccaa atgaaaattc aatgttgtca gtttccttta
    26821 taaaaaatat tttaaatgca gaaaacaaag attttgctaa aaatatattt aactatgggg
    26881 aagaaagaaa aaacaataag caaataaagg atataaatac agccatctac ataccaaatc
    26941 ttataagaag caaaaatgag ttgcttgttg atggagatat aatattcaat ataagttttt
    27001 ataattattc tatgttcagt aaattataca atggaatttt aaaagtaaag gagctagaat
    27061 ataaaggtta tagatttaaa ataaaaaata taaaaataca taaagaacac gagataaaag
    27121 aaaatggtgt tatatttaaa actatgtctc ctataatagt aaagaataaa gaaggtaaat
    27181 atttagatgt agaagattca aattatatag aatgtttaaa ctatatagct aatctaactc
    27241 tggagagtat aagaggtagt gggcttagaa agccattgga atttatacct cttaacttca
    27301 aaaaaagagt gttaaaagaa aagattcgtg gatttaaagt gagagaatat tactatataa
    27361 atgcttatgc aggaacattt tttttaaaag gaaatatgga agatttaaat gcactgtata
    27421 aaatgggaat tggatataga aggacggaaa atgcaggaat ggttgatata ttaaagtagc
    27481 gaggtgagag aaataaaagc taaattttat agatcaggtg atattgtaag agatattggc
    27541 attgtttact tttatgaatt actaatggat ttaaaaaacg aactagaaaa aaatgattat
    27601 aagctaaaat ttacttgtga gttaaataga aattatttat ctttagaatc tgcagaatat
    27661 atagacccta agtatatatc tgattatata ttagaaaatc aactttttaa ggtatttaaa
    27721 aatgggaaaa aggaaacctt agaaaaaata tttagtaata tagataattt aacgtatgta
    27781 aattttattg aagaattaga taaatcttct gcaactgaga aacaaaagga agggattaaa
    27841 aaagatttta aaaacagaag atttccatat gttagaaata gtggtaagta tggattaaac
    27901 acgagtttag aaaatatgga aacaaatgta aaaagaatag tagatactgt aattaaatca
    27961 aatatagaga tagatgaaat agataaactt gatttgacac aatatgaaga aaaagatagt
    28021 gtatgtaatg tttgtaatat aaataaaaca acaaaactag atattgattt aagagagcga
    28081 aaggactcta agtataactt tttatttaga ggtgcagaaa aatcaggctt taaaagaagt
    28141 ggacaagttg aaagtaatat ttgttttgag tgtgaatttt tcaacttaat gtgtctttta
    28201 tatataaatt taaaaagacc aatggttttt gcatatacga atgatttaag ggaattagca
    28261 tttttaaatc ataagataat gcttaaaaga aagatgtatt cagataaatc attttataaa
    28321 aaacttttgc atgaaaaaat aagttcgatt agactatata ggtttgatat tgatacgaat
    28381 aaaggtataa ttctaaagtt tgactctatt atagagtata aagaattatt aaaaaaaatt
    28441 gaacttatag atattataga taattataat ttttcaagag agccagggaa tactagaaat
    28501 ttgggtaaaa aaatgattga taatgggaat ttatcaaatt taaaagaatt attacttgat
    28561 aatataagtg ttttaaggca actaactgga acttctcgag aaatggattt tgtatctagc
    28621 tcatataata tcggtttata tataaaattg tgttggattg tagatggagg ggaggagtat
    28681 aaaatgaaaa actatatgga aagtaattta atatattcaa gagtaggtaa agacttattt
    28741 aataaaatga cagatgaatc cagaagaaaa aatttttcta tgaaagtgat tcaactactt
    28801 aagtctaatg atagaactca gctatttcaa actataatgc atgtgttagt ttctaatgga
    28861 atcttaatag gagaagggtt tgtagaaggt ctaatgcaga gttctgaatt agaattaaat
    28921 acgaatgtag gtctatttat tcaagaaatc atgaaataaa aaagggggaa aatttaaaat
    28981 gaatgaaaaa agagtaaaag gagtaacatt aactatactg acagaaagcc cagttccact
    29041 ttcatatgac caagggtatg gaaattatac accaataaaa aaagaacaat atagagaaaa
    29101 aatacatgca aaaactagta tagccacaat aacatatgat ttaagaagaa tgctccacca
    29161 agaatatggt tggaatttaa gtaatattat ttttgggaaa aaaggaaata tatatccatc
    29221 tattaagcaa aatgtaggat gtgtagatga aaatggatta gagactgatg tatttggtta
    29281 tttgatacca cttaaagata aaggttctat aagtaaggct tctccgctta gaataatacc
    29341 ttttaggtca ttaaattcat atagaggttc aacacagttg ataacaaata gaggtttttt
    29401 aagtagcgaa tttggtagaa aatattatga tgagaaggaa gaaaatgaag tacttagaga
    29461 tgaaaacttt ccaacatcac aagcattagc aatagaagag actttatcag attactacgt
    29521 ttatacaata acattggagc ttgatagaat aggagtagta gaagtagaag atggaaagtt
    29581 gcttttacca gaagaaagaa aatttatgag taaggagtta agagaaaaag cagtaaaaga
    29641 tatattagat gcaattactg tgtttacaag aaacataaaa cattcatcag tactgttaaa
    29701 accacttgca gtaatgggtg gagcttttga taaagttgta ccgttttttt gggatgatgt
    29761 agattataat gctgatagtg gtgagataaa tttagaggga gtcatagaaa ctatagaaag
    29821 ttatagcctt aaagaaagta acacaatact tgctatcaat gacaaattaa aaatttctaa
    29881 taaaaaggaa ttaaataagt ttaatttaag taaataccct gtaaaagaga ttaaaaatct
    29941 agcagataga ttggaaattg gagaagataa tatgtggtat ttaaaagagt aggaggtttt
    30001 ttgaattatg aaagtattac aatgtaaact taagcaacca actgctcact atagagaccc
    30061 aaaagtattt caaaatgaat atataagtac tttaaattta ccatctaaga ccacaataat
    30121 gggaatgata acgtatttat gtgatagaag attaaactct gatatagata taggaataat
    30181 tggaacacat catcatagag aactagaatt ttcaagagga gagaacattg atttttggaa
    30241 tgaatataca aatatgagaa aaggcaaaga taaagaaaag tttttgcttc aaggaaacta
    30301 ctatgattac tataaagaac ataaagctca aaatagcata ttaaactatg aagttcttaa
    30361 agaagtagaa ctgactatat ttatttcttg caaggatgat gaagaacttg aatttattaa
    30421 aaaaaagctt gaaagtccat gtaaatatgc aaatttagga agaaaagaag attttgtaat
    30481 accaagtgaa aaagggtgtt ttgtgaaaga agttgagtta aaagaggtta tgcctatgaa
    30541 tactcgtgat gctataaaag aaaatataaa attaaagaat acatatgtaa gagttgactt
    30601 aagagataaa gagaatgtcg agactataat aaatcaaggt gtattgattg cattacctta
    30661 taaatataaa gatttagaag caaatagaga tgatagagtt tatgagtttt gccattatat
    30721 atatgttgat aatgatggta tatatcctaa aaatattaag gtaaatgtat gtaatgtatg
    30781 tattgataca aaagaagttt ttacatggct ttaaattatt ttgaaagata ggtgtttatc
    30841 atgaaaaata tcatgcaaaa agaaatatta gctaagtcat ataatttaag aactaatcaa
    30901 caagaacaaa cattagttga acatattaag gatttatttg aagtattaga atctatatta
    30961 gaactaaatc tatattcaga taaagatgta gagatactaa aaatatgttg tgctttgcac
    31021 gatttaggaa aaattaattc aattatgcaa caaaaaatgg agattaataa taaaatatcg
    31081 tatagttgct cagaagaaga gaggaaaaag ttagagtctg acaaaaaatc tttaaaaaag
    31141 atagcaagac acaatatatt ttctggagca tttttaaaag atatattaga aaaaatgaac
    31201 ttgttagaag aagataagtt atatatatat aaaagtataa tgttacatca tggaaactat
    31261 gaagattata tgaggttaag tactagtaaa gttcaagaag aaatttatga ttatattgaa
    31321 aaaggcattt tagaaaaaga agagtttaat ctgaaagata tagaaagtta tataaatgac
    31381 attttaaatg tagattttaa atttggtgaa gatgtattgg attatgatta catagataaa
    31441 ttaagtgaaa gtatgtttat agacagtgat tataacaatg gtcaaataga tgattcagtt
    31501 ttaaattata gaaagtttaa gtatatttta tataaaggga tgctgaactt aatagaccat
    31561 agtgcatcaa gtagacagaa aggtataaaa ttttataacg attttactga tgaagaaatt
    31621 gatgatatga ttttaaatga gatatataag agtcagggta atttagaaaa aaataatata
    31681 gagttcaata ctatacaaaa aaggttgaga gaactttctg gaagaaatgt tctgactgtt
    31741 gcatttacag gttcaggaaa aacagctgca gattatagaa agacttttaa aagaaaattc
    31801 tttttagtgc caaataaaat atcagcagaa agtttttata gaaaaaatat ttttcaaaat
    31861 aaaaatgatt tagatacaag aagtagtaat gattacattg gattacttca tggagatata
    31921 aacttatatt cagagaatga agataatgga gaacatgatt ttgtattgac attaagggat
    31981 atagatttga gtataaattt ttgtaaacct tgtgtaattg caacagttga ccagttatta
    32041 cttagtatgt ttaaatttcc gggatatgaa aaaatattcg cagccgtaaa aaatgcatct
    32101 ataactgttg atgaagtaca tttattggaa ccaaagatgt ttttgatttt gatatatttt
    32161 atgcagtttg catgcaagta cttagatgtt gattttcact taatgacagc tacacttcca
    32221 aaatcatata aagagcaaat gataaacaag ggaattattt ttcaagaaga aagtaatgaa
    32281 aatgttactg ataaaggaga aatagttttt attgagagca acaaggaaga agaaatttgt
    32341 gaaggtaaag atgtaaaggt ttcttttata aaggaaaaag aaatcaagtc aatagttgaa
    32401 ggtgctttag aaaataaaca aaaaattctt ataataaaga atactattga ttcagtcaat
    32461 agaacatatg agtttttaaa agaaaattta tctgataagt atggtggtgt agatatagat
    32521 gttttacata gtagatttaa gttcaaagat aaaaaagaga aatatagtaa aatattaaat
    32581 ggagagggtg atatttggat atcaactcaa tcagtagaaa tatccttgga tttggatttc
    32641 aatataataa taagtgattt agcaactatg gatagcttaa tacaaagaat gggtagatgc
    32701 aatagaaata ataaatatga atatggaaac ttttatattc tacctagtga agataaaatt
    32761 tatgataaca aacttaagaa cacaactaaa aacatcttaa aggacatatt aaaaactaac
    32821 tctattttta caatggggat tagaaaaact attttggatg attactatga taatagtgta
    32881 gttaaaaact attttgaaga gaattttata agttgtgata aagaaattaa gaatatttat
    32941 ggtataaata aagaaatttt tgatggattg gatttaattt ttaattttga accttacaaa
    33001 aatattgttg atagtaagaa ggaagcagct aaaattttta gagatgttga tgttagttac
    33061 aaaattattc tggaagaaga tttttataag gaagataggg agttacaaca agattcgatt
    33121 caagtatcag ggtttatatt taatagatta tattattatg gattaataag caaagtagaa
    33181 ggatacatgg tactaaaatc tagtagtaag tttgagtata atagtactgt aggcttaatt
    33241 ttgaagtgag catatgttta aatataatat acctttagac attgaaaata caaaaagtac
    33301 tactgtaagc ccactgtaaa aactattttt taaaaggaaa aataagttgt gagatagagt
    33361 gtctacaagt gtatatagat aataaatttg aggtttaaga ttaactaagt gggtttcagg
    33421 tacatcgcga aaataattat aagttggaga ttttgtatgt ttaagattaa ctaagtggat
    33481 ttcaaatatt gatatagtag atataataga agttttatta ttaggtttaa aattaactaa
    33541 tcggattttt aaatattctg actattatat aaggatatta ttagcttgaa tttaaaacca
    33601 ataatataaa acaacaaaat attatattta tgatataata aaagttatgg aaaaacagga
    33661 atattactat agagaagaga ggtaaactta tgaataattg gatagaagta actataaaaa
    33721 caacaacaga agcagttgaa cctataacga atatactata tgaacaagga gctggaggag
    33781 ctgtaataga agacccaaaa gactttttat tccaaaagaa aaatgaacta gactgggatt
    33841 atgttgaaga agaagtattt aaaaagaatg aagaagatga tgttcttata aaaacttatg
    33901 tttctgaaga aaaaaatgtg atggaatttg tcgaaataat aaagcaaaaa gttttaggat
    33961 taaaagattt tggtatagat ataggtgaag gaagtgtatc attggaccaa gtaaatgaag
    34021 ctgattgggc aaatgcatgg aaagcatact acaaaccaac aaaagtagga caaagggtag
    34081 tagtaaagcc tacatgggaa gattatgcta tgcaagatgg ggatttaatc atagagcttg
    34141 accctggaat ggcatttggt acagggacac atgaaactac tagtatgtgc attagagaat
    34201 tagaaaagta tgtgaataaa gattctaaag tatttgatat tggatgtggt agtggaatcc
    34261 ttgcaatagc agcagctaaa ttaggagcaa aagaagttgt agctgtagat ttagatgaag
    34321 tggctgtaaa agttgcaaaa gaaaatgttc ttgaaaataa agttgaaaaa agtgtatctg
    34381 taatgcatgg taatttaact gatgtaataa aagataaagc ggatgtaata gttgcaaata
    34441 taatagctga tataataaag atacttgcaa aagatgttca aaactttatg aaagaagatg
    34501 ctatatttat atcttcaggt ataattttgg ataaagtaga agaagttaaa gaaagcttaa
    34561 ttgaaaatgg atttgaaata gttgaagttc aaaaattagg tgaatggtca gcaatagttt
    34621 ctaagcttaa aaaataaggg atatggtgac gtataatgga tagatttttt gttgaaaaaa
    34681 ataatataaa tctacaagat aaaacttgca ctatagaagg tgaagatgta aaacatattt
    34741 caaaagtttt aagatgcaaa ctaggtgaga aacttgaaat atgtgacaag aataataatg
    34801 agtatatttg tgagataatg aatatagata agagtatagt taatttagag attttagaaa
    34861 aagttgatat aaatagagaa tcagaactta aagtaaggtt ataccaggga cttccaaaag
    34921 ctccaaaaat ggaaatgata cttcaaaaat taactgaagt gggtgtagaa gaaataatat
    34981 tagttcaaac taagagaagt gttgttaaag ttgatgacaa aaaagaagat aaaaaatttg
    35041 aacgttggga aagaataatt tatgaagcag caaagcaaag caaaagaggt aagataccaa
    35101 aattaagagg tgttttaagt tttaaagaag ctttagaaga tatgaaaaaa aataatgtaa
    35161 atatttgccc ttatgaaaat gaaagaacag tttcaataaa acatgcacta aaaaaatgtg
    35221 attctaatat agatagtgtt ggaattttta taggtccaga aggtggattt tctgaagaag
    35281 aaatagagca aatacaaaaa aataattgta atgttgtatc attggggcct agaatattaa
    35341 gaactgagac agcttctgtt gtagcatcaa caatagcttt gtatgaacta agtgatttag
    35401 gaggagaaaa ataaattgaa aaaagtagct ttttatacac ttgggtgtaa agttaatcaa
    35461 tatgaaacag aagctatgct agaactattt gagaaagatg gttatgaaca agtcaatagt
    35521 gaagagtatg ctgatgttta tgttataaat acatgtacag ttactcatat gagtgataga
    35581 aaatctcgtc aatatataag acgagttaaa aagaagaatc cagatgcaat aatagccgtt
    35641 gtaggatgtt attctcaggt atctccagaa gaaattttgg atatagaaga agtaaattta
    35701 gtcatgggta caaatgatag aagaaagatt gttgaagaaa ttaaaaagat aaattcaagt
    35761 aagaaagtta gtacagttga tgacataatg aaagttaaag cttttgaaga aatagaaatc
    35821 agccaaacta atggaaagac tagagcattt atgaaaatac aagatggatg tgacagattt
    35881 tgtacttatt gtatcattcc ttatgcccga ggacgcgtta gaagtagaga tattgatagt
    35941 atagtcgatg aagtgaaaaa attggctaat aatggctata aagaggttgt attaacagga
    36001 atacatgttg cctcttatgg aaaagattta aaggataggg atattaaact tcttgatgta
    36061 ataaaacaaa taaaccagat agaaaaaata gaaagaatta ggcttagttc tgttgaacca
    36121 attttattta cagatgaatt tgtaaatgaa gttttaaaga tggataaagt ttgtcctcat
    36181 taccatttat cacttcaaag tggatgtgat gaaactctaa aaagaatgaa tagacgatac
    36241 acaactttag agtacaaaac tattgtagat agattgagaa gtaaaatgcc agatgtggca
    36301 ataactacag atgtaatagt aggttttcct ggagagacaa atgaagagtt taaaaaaacg
    36361 tacgagtttt taaaagaaat agagctttca caaatgcata tttttaaata ttcaccaaga
    36421 aaaggaactc cagctgcaac tatggaaaat caagttgacc ctcaaatgaa acactttaga
    36481 agtgaacaac ttttaaattt aagtaaagta aattttaata aatttgcaac taaatttatc
    36541 ggccgtgagc tggatgtttt gtttgaacaa aatatagaag gcaataaata tgagggatta
    36601 acttcaaatt atataagagt tgttgttgaa agtgacaaaa atatacaagg tcagatatta
    36661 aaagtgaaaa taaatgatgt aaaagatgaa tatgtagaag gaattttgct ataacatgta
    36721 gaaattataa tatttataag ataggaggtg gaaagaatgg attgtatttt ttgtaaaata
    36781 gcgaacggag aaataccttc aactaaagtt tatgaagatg atagagtctt agcttttaat
    36841 gacttgaacc cagttgctcc atatcatata ttagttgtac ctaaaaaaca ttatgatagt
    36901 ttaattgata ttcctgataa agagatggac attgtgtcac atattcatgt tgttattaat
    36961 aaaatagcta aagagaaagg ttttgaccaa actggattca gagtcataaa taattgtgga
    37021 agtgatggag ggcaagaagt taaacatttg cactatcata ttttagctgg taaaaagctt
    37081 cctaattacg aagcgggaca aaactaaact aaattattga aaaattgagt tccttcttga
    37141 taaaaatact tgataaaagt aaaaatttag tttataataa acaaagtgtt aggttgtaat
    37201 gcatattatg caaaaagaac atagttacag tcccagcata attgcgctat tggagggagg
    37261 gaaaaagaaa tgtcagaagt aagagttaga gagaatgaaa cattagacag cgctttaaga
    37321 agattcaagc gtcaatgtgc aatgtctggc atcatgtcag aagtgagaaa aagagagcac
    37381 tatgataagc caagcgttaa gcgtaagaaa aaagctgaag cagcaagaag aaaaaacgct
    37441 aagaaatagt agtaaataag aataaagagg tgaaggggat gtcccttaag caaaagttac
    37501 aagaggattt gaaatcttcg atgaaaaaca aagatacagt gagaaaatct gtagtaactt
    37561 taataagagc ttctataaag cagtatgagg ttgataatag ggtcgagctt gatgaagatg
    37621 gaattatcga tgtaatagca aaacaattga agcaacgtag agatgcatta gttgaatttg
    37681 aaaaagctgg aagagaagac ttaataaaag aaacagaagg tgaaattgaa gttttaaaag
    37741 agtacctacc tcaacaatta agcgaagaag agttagaaga aattgtaaaa tctactatat
    37801 ccgaagtagg ggcaacttct atgaaggata tgggaaaaat aatgtcagtt atccaaccaa
    37861 aagttaaagg aagagctgac ggaaaactta taaataagtt ggtaaaacaa aacttacaat
    37921 agattgatat agcctagaga gaaatctcta ggctttttat agtttttggg aataacgagt
    37981 catatttgag catatctata atcaaagaaa attttgaaag aaggggaaaa atttgattat
    38041 atcagtaaaa gagattagga attttttgat aaacatattt ataccactaa taataggtta
    38101 cttaagtagc atcttaacaa tgattatcag tggaacagat atttcaacct attatttaca
    38161 actcacaaag ccaagctttg caccaccagc ttttatcttt cctatagttt ggactatatt
    38221 gtacatattg atgggtatat cttcatacct aatattaaga aagggttaca acttacctaa
    38281 ggtaagagat gcaatttttt attatggatt acaacttgta ttaaacttta tttggagtat
    38341 actatttttt ggatttggac ttagatttac agctttgata gatataataa taatgatttt
    38401 ggtttctata ataatgattt cgaagttttc taaaatagat aagagatctg gatacataaa
    38461 tttgatttat ttaatgtggc ttgtttatgc aggatttttg aactatttta tatggtttat
    38521 taataaatag aatgtaaaga ttttgtaaat taccctgtac ttttaataat atcataacaa
    38581 acgaaaaata aatagactat taaaaataat cattttcata tacaataata ttaatttaaa
    38641 tacaataaat ttataaaaat tgtatttaaa gattaacata ttaaagtatt agaattggaa
    38701 aggcttgaag ggagaggttt ttatgcaaat aatagtatct gggagacaaa tgaaattaac
    38761 tgacggaata aaaggatacg tagatggaaa attaagtagg ttagaaaaat accttgatcc
    38821 tgaatcagaa gtgaaagtaa cagtaagtgc gaaaaaggat agacaaaaag tggaggttac
    38881 aataattcct attaacggtc aaataataag agcagaagat gtagaagatg acttatatgc
    38941 agcaattgat attgtttgtg acaaattaag tagacaagtt gtcaaatata aaactaaagt
    39001 taaagacaaa gttcaaaata ataagagcat aagatttgaa aatcttgact ttatagataa
    39061 tagcagtgaa tttgatgatt atgattatga tgatgaagaa gatgaaaata tagtaataga
    39121 aagaagaaag aaatttaatg taaaacctat gagttctgag gaggcgatac ttcaaatgga
    39181 actagtagga cacaatttct atatgtttag aaaccaagat aattttgaaa taaacatagt
    39241 gtataagcgt aaagctggtg gatatggttt aatagaacaa gattaataaa aattgtatac
    39301 gtttagattt tatataagaa aaaataaaaa ggtaatcatg ttgatttttt atcaatatga
    39361 ttaccttttt attgtttaaa taatatatta gatattaggt atttattata taaaaatttt
    39421 aaaactaatc tgttacaaca tcaataaagt gcataaatac ctatttcaag cataaaatta
    39481 attaaggggg taatgcttat gttagaaata tccactgatt tacaagtaaa tcaaccagtt
    39541 attactgtta caagtaatac gtttattagt atagaaaatt atctatcaat attagaatat
    39601 gaagttgatt taataagaat aaagactaaa gttaaaacaa ttaaaatatc tggggataaa
    39661 ctttcactta agtatattac tgatagtgag ataggtataa aagggattat ctacaatgtt
    39721 gagtatgttg attaggagga gatacacttg ataagtttta taaggggata ctatgttata
    39781 gttgtggaag gagtaggact cgaacaattt ttaaatcatc ttataagaaa tgggataagt
    39841 gtttacaatg taactagaat aaaaaatact aaaatggagt ttcatataga tagacaagat
    39901 ataaaagaat ttaagaatgt ttatagagga agtaagtttg atataaaggt aaagcaaaaa
    39961 actggggtcc cttttattat caaaagagtt tataagcaca aaggtatgtg gatttgtgct
    40021 cttgtttctt tatttttatt gatgtctaca tcgcagtttg tgacagatgt atatatacaa
    40081 tctcctgaag gtataaaaaa agaagctctt agaaatgagc tttataaggt tggagtaaga
    40141 ccaggagtct ataagaagag tattgataga aaagaagtta gagaccatat gatgtcaaaa
    40201 tttaatgatg tggcatattt atctatcaat gtaaaaggaa caaatatatt tgttactgtt
    40261 acaaaaaagg cagaatccct aaaatctaca gaccaatcaa attattgtaa tgtaattgct
    40321 ttaaaaaatg gtataataga aaaggtaatc cctagaagtg gtaaatcagt tattaaatct
    40381 ggagacatag tccaaaaggg agatgtctta ttaaatggag ctaataccaa gtcaatacca
    40441 gaagtatggg caagtacttt ttatgagtcc acaaaaaagg caagttatgt agatactgta
    40501 aataaaaaaa ctggagaaaa gaaaaatata tatacactaa gtttttatga taaagagttt
    40561 acaatgagaa aaaatataaa atataaagat tatgttgttg aaaataaaga aaaaaaacta
    40621 tcaataggaa actatacttt tccaataaag ataaaaacga gtacttttta tgaaacaaaa
    40681 aaggttgaag taaaaaggaa taaagaagag ttaaaaaaag aattatctga aaaagcttta
    40741 aaagagttag aatacataat cccagcttcg gctagaataa tagatgtaaa acataacttt
    40801 aaggttaata aaaatatgtt agaatattta ataacagttc aaactagtga aaatattgct
    40861 aaaatttatc ctcttagtaa gtcagaagca gaaaggttta taaaggaaga aagtaaacca
    40921 gatgaaggtg aagaggaagt tccttccaat ccagaaaaaa gaccattaaa tgatataaga
    40981 aatgagtttg atgaggataa taaggataat aaagaccaaa acaattctga tgaaaataat
    41041 agtaatcaaa atagtaataa tagtcaaaat aataatagca ataacaacta ataaaattaa
    41101 acttaaaatc ttaggaggac taattcaatt gataatacaa aagaaattta caatagcaga
    41161 tggtaacttt gaaagagagt tatttggcaa ctttgatgaa aatgtaaaat taatagagaa
    41221 gactttaaat atagatgtaa tacttagaga aggcaatata attttaattg gagaagaaaa
    41281 aaatgtagat tctgcattaa aacttatgaa tgaattacac caaactgtat caaatggtaa
    41341 gcatttagat aagcagagta tttcttattc attatctctt ttgcttgaag gaagtgaaca
    41401 aaaaataaaa gaacttgagg ggacaatcgt cataactcaa aggggaaaag ctgttcaacc
    41461 taaaacttta ggtcaaaaag aatatataaa acttatagaa aataatgata taacttttgg
    41521 tgttggacct gctggaacag gaaagactta tcttgcggtt gctatggcag taaaagcttt
    41581 taaaagagat gaagttagta gaattatact tacaagacca gctgtggaag caggagagag
    41641 tctaggtttt ttaccaggag atttaaagga caaagttgac ccatatttaa gaccactata
    41701 tgatgcctta tttgaaatgt tgggtgctga taaatttaat aagtatttag aaagaggtac
    41761 tattgaggtt gcaccacttg catttatgag aggtagaaca cttgataatt catttataat
    41821 attggatgaa gctcaaaata ctacttctga acaaatgaaa atgtttttaa ctagacttgg
    41881 atttggctct aaagctgtag ttacaggaga tgtaactcaa acagatttac cacaaaataa
    41941 gaagagtggt ctaatacaag ctacagagat ccttaaaggt gttccaggta ttgggtctat
    42001 aatgctaaca gatagagatg ttgtaagaca tgagttggta cagagaataa taagagctta
    42061 tgagaagcac gacaaaagag aagaatttaa aaaagaagaa cgtaaaaaag tgaaaatgca
    42121 ggaaaagact ttcaaaagaa agtaaaagtg tagtaatata gatatatagc attattaatg
    42181 aataaaaagt aggtgtagtg aatggatttg atactagatg atagacaaga taaattagaa
    42241 gtaagtgagg agttaattga aaagataaag gatataatca tagaatgttt agattatgaa
    42301 ggatatgatg ataattatga agtgagtctt tcttttgtag ataataaaga gattcatgaa
    42361 ttaaatagag aatatagagg agttgataga gtgactgatg ttttatcatt tcctctttta
    42421 agtgatgatt ttgaggatgt tgagttagaa gaagaatccc taggtgacat agtagtatct
    42481 cttgaaagag ccttagaaca gagtatagag tataaccata gttttgaaag agaagtatgt
    42541 tttttaatat gtcatagtat gtttcatctt ttaggttatg accacgatac agatgaaaat
    42601 acaaaagaaa tgagagaaaa agaagaacac atattaaata agttaaacat aacaagggag
    42661 tagtttttat gaaaccagaa aaaactcgtc aaggtataat taaagcattt aatgcagcta
    42721 tagaagggat attgtataca tttaaatttg aaagaaatat gaaaatacat tatttaggtt
    42781 cagttgctgt tcttataata agtttgtttt ttaacttttc aaaattagaa atgataatgt
    42841 tacttatgtc catatgctta gttgtagttg ccgaaatgtt taatacagct atagaaaaag
    42901 ctgttgattt agtaactgat gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag
    42961 ctggaggcgt tctagtggca gcactaaatt cagtagtagt tggatatatt ttgttttatg
    43021 ataaattgac agatatatct ggaatattaa tttataaaat aagagaatca gaattacata
    43081 taaccttgat atgtatatta ttggttctta tagctgttgt agttgtaaaa gcattgactt
    43141 ctacaggtac tcctctaaaa ggaggaatgc caagtggtca tgctgcctta gcatttgcta
    43201 tagcaactgc aattacactt atgactgaaa gagttgttgc ctctactctt gcatatataa
    43261 tggctgtatt agttgcccaa agtagaattg aaggaaaaat acataccttt tgggagacta
    43321 ttgctggagc tttattaggt gtgttgatag ctatattagt atttcagctt ggaatgttct
    43381 ataattaaaa taaatgtaca taaaatattt aaaataaaac aaatctgtgc taattaaatt
    43441 tttatgatat aatgtagagt attaaataaa aataactttt aattattgta tacaggagga
    43501 gttgttgtgg ataacataga attgttaagg ttggcagaag atgccagaca acattcttat
    43561 gctccttact caggttttag agtaggtgca gctcttttaa ctaagagtgg taaagtttat
    43621 actggatgta atatagaatg tgcatcatta ggtggaacaa attgtgcaga aagaacagct
    43681 atttttaaag caatatcaga gggtgataag gatatctata aaatagctat agcaagtgat
    43741 aactcagaaa ataatgagca aacatatcca tgtggaatat gcaggcaggt aattatagag
    43801 tttggttctg atataaaaat aataacggga tatactaaag gagaggtgtt tgagcatagt
    43861 ataaaggatt tgttgccaaa ctatttctct ggaaatgatt taaaataaat ttaaaagtat
    43921 aaataaattt aagtaattaa aacaagaaga taaaaagtta ggagacagtt aatgttcaaa
    43981 tcaggttttg tcagtatagt gggtagacca aatgtaggaa aatctacttt aatgaataat
    44041 gtagttggag aaaaaatagc tataatgagt gataagcctc aaactacaag aaatacaata
    44101 caagcagtat atacagatga agaaatgcag atagtattct tagatacacc cggtattcac
    44161 aaaccaaaaa ataaattagg tgaatttatg gtaaaggcag caacagaagc ttttaaaaat
    44221 gtagatttaa tattatttgt agttgatgat tctaaaaaaa taggtcctgg agatagaaag
    44281 ataatagagg atttaagaag tgttaagaca ccaataattt tagttgtaaa caaaatagac
    44341 caactagacc aaaaagatga gctatttgat ataatcaaaa tgtatgatag agaaggtatt
    44401 tttaaagaaa tagtaccaat atcagcatta aaaggaaaga atacggatac cttaataaaa
    44461 gttatacaaa attatttaga agaagggcca aagtattttc cagattatat gattacagac
    44521 cagccagaaa gagtattaat agcagaactt ataagagaaa aagttcttca ctatcttaat
    44581 gatgaaatac ctcatggtgt agctgttgaa attgaaaaaa tgaaagcgag aaatgataag
    44641 gaaatagtag atgtatctgc tgtaatatac tgtgaaagag attcacataa gggaataata
    44701 ataggaaaaa atggtaggaa actaaaagga ataggtaagt cagcaagaca agatatagaa
    44761 ttgcttttag gttcacaaat aaatcttcaa ttatgggtta aagtcaaaga aaattggaga
    44821 aatttacaaa attatataaa taattttgga tataatgata aataaaggtg ggttatatgg
    44881 ataggaaact ggatgcttcc caagatttat tatatgaagt aaaatcatta attgataata
    44941 ataaagtatt ggagttaaga gaattaatag aggaatatca tataattgat atattcgata
    45001 taatggaaaa tttagaggaa gacatgaaaa ttcaattatt tgaagtactt cctttagata
    45061 tggcatcttc tatattagaa gaaggtagtg tagagttttt tattagtatt ttatctaaac
    45121 tggatgtaga acactcgaaa aatatacttg aacttatgtc tcttggagat atggcagaca
    45181 agttgagtga acttgaagaa gaagaaagag aacacataat aaatcttttg aatcaagaga
    45241 atgccgatta tgtaaaagag ttactattct atgatgaaga ttctgctggt ggaacaatga
    45301 ccactggata tatttctata aataaagata tgacagctct tgaggctata gaccatatga
    45361 gagaagaagc tgaagaagct gaaactattt attatatata tgtggttgat gatgaagaaa
    45421 aactagttgg agtattgtca ttaagagaat taataatagc tagagacgca aatatagtag
    45481 aagatttgat gagtgaaaat ataatctctg tttatgtaga tgaagataga gaagaagcag
    45541 taagattagt atctaaatac aatttaatag caattccagt agtagacaga caagaaaaat
    45601 taaaaggaat aataacagta gatgatataa ttgatgtaat ggaagaagaa gcaactgaag
    45661 atatgtataa atttgcagga agttcagaac atgaaagaga agttgctgaa aaagaaaacc
    45721 ctacattgag agagcaaata atatcagcac ttagaggacg attaccttgg ttaattatta
    45781 ccttggttgg agggttgttg gcttcgttaa tactttctaa cttagactat ataatgaatc
    45841 ctgtatatgc tccattagta ttttttatac ctgtagtaat tggtatgggt ggaaatattg
    45901 gaactcaatc atcatctgtt acagtaataa cattatctaa taaggattta aattttagca
    45961 atgtggttag agaaggtatt gtaggtatta ttacaggtct tttgtgtagt ataataacag
    46021 gtattgtaat ttactttgtt atgagaaagt tagacattgt attaattgtt tcaatatcat
    46081 tatttataaa tatggtttta ggtgcaacaa taggtgcgtt tatgcctgtt ttattaaaaa
    46141 aaatggatgc agacccatct acagtttcat caccaattat atctacagct cttgatataa
    46201 ctggtatagc agtttacttt ataataacaa cggcattatt gtcaaaaatt gtttaataac
    46261 tttatgtatg atttatcctc ttcttactaa aaatataagt ataaatgtaa gaagggggtt
    46321 ttttaaaaat gaacaatatc agttgggaag tttttaaaaa aacaggtagt atagatgcat
    46381 atttatattt ctgtgattgc aaaaacatac atgaagaaat tgaagaagta agggagaaaa
    46441 aagaagacga tgataatcct gaacacccag gggatagtac ttaaagctat aaggtataag
    46501 gaaagtgaca taatacttac actatttaca agaaagcttg gaaaagtatc tgcaatagca
    46561 aaaggagcta aaaaaaataa gagctctttg ctttcatcat cacaattatt ttcttatagc
    46621 aattttacct taaaaaaaca aggtaatatg tacaaggtta ctcaaagtga aataataaaa
    46681 agtttttata atatatccta tgatatagag gcattttctt atgctacata tattacaaaa
    46741 ttagttgaaa attctatact tgaaaatcag actaataata ggctatttat attacttgcg
    46801 cagactctgt atttgtatac acaggataat acagacaata ggtttataac agcagcattc
    46861 gagttaaaat ttttagacta tataggattt aaacctatag taaataaatg cattaattgt
    46921 gaatgtaaaa ttttacaaaa ttctgtgttt aatatctacg aaggtggtat actatgtagt
    46981 aagtgtagta aactttttga taataatata aaattagact taacaactat aagtcttatg
    47041 gaatatgtat taagaaatga tattttaaca tgtagtaaag ctaaagtatc aaaatatata
    47101 acacatgaac ttgagaatat tctagacaag tatttgaaag tttatgttga taatattaat
    47161 tttaaatcat tacacgtatt gcaaagtgta aaaaataata agggagtgga taatgatgag
    47221 tagtaatata actattgaaa tggttgattc agtctttgaa agagttccag gagcaactta
    47281 tgcagaagca aaagaagcat tagtaatgtg caatggagat gtaattgagg cagtaatata
    47341 tttagaaaat aaaaaaaata cttgtaattg taaagcaaaa tctgctaaag aaactatgga
    47401 agaagtattt ggcaaagatg gagaaaatat aaagtttcaa ttaaaagagc ttcttagaaa
    47461 aagtagtgta gtgagaataa tagttgaaaa agatggaaaa actatgatga acattccttt
    47521 aacagttgga gttgttggat tagcttttgt accattagct actttagttg gattatcagc
    47581 tgctgttata agtaaatatc gtataaaagt tcaaaatgaa gaagatggag aaattgtaga
    47641 tttaggtgaa ttgaatgaag aaaaacttca cgttttgaag aatatgataa ctaatgcagc
    47701 aaaagatgtg aaggatgtag tagttgacaa aaaagaagat gacaaagatg taactgctga
    47761 ccttatgaaa gaagaatctg aaaagaataa agaagacgaa gacgaaaata aatagatata
    47821 aagtgacttt taaaaaatga agagaagata attataagaa ttatcactaa cattgacaaa
    47881 ttttatagaa tttgatattc taaaagttgt agaagaacta ttaaaatagc ctttcgtgta
    47941 gacgagaggc tttattgtta taggagggat ttttatgaat tttcaagaga tgatactggc
    48001 gttgcaaaaa tactggtcaa agcaaggttg tatcatgatg caaccttatg atattgaaaa
    48061 aggtgcagga actatgaatc ctaatacttt tttaagatca ttaggacctg agccttggca
    48121 agtatgctat gtagagcctt caagaagacc agcagatgga agatatggtg aaaacccaaa
    48181 cagactatat caacatcatc aattccaagt tatactaaaa ccttctccag ataatataca
    48241 agaattatat ttggaaagtt taaaagagat tggaattgac ccaagtgagc atgacataag
    48301 atttgttgaa gataactggg aagctgctac agttggtgct tggggacttg gatgggaagt
    48361 ttggctagat ggtatggaaa taactcaatt tacgtatttt caacaagtag gtaatattga
    48421 atgtgagctt gagacaggag aaattacata tggattagaa agattagcta tgtacattca
    48481 agaagttgat agtgtttatg atttaaaatg gaatgataaa ataacttatg gtgaagtatt
    48541 taataaagct gagtatgaaa actcaatgta cgcatttgaa ttatgtgatg cagatatgtt
    48601 gtttaatctg tttgatatat atgaaaaaga agcattaaga cttatggaaa atggccttgt
    48661 aataccttca tatgactatg tgttaaaatg ctctcatgca tttaatacat tggacgcaag
    48721 aggtgcaata ggagttagtc aaagagcttc atttataggt agagttagaa atatggcaaa
    48781 gactgttgca gaaacttttg taaaacaaag agaagaaatg ggattccctc ttttaaagga
    48841 cggtgacaaa taatgaaaaa ttatctttta tttgaaatcg gtgttgaaga aatgccatca
    48901 agatttgtag gcagtacact agaacaatta aaaaacaatc ttagtaaatt atttgatgaa
    48961 aatagaatag cttttgataa aataaatgcg tatgggacac caagaagact tactttagta
    49021 gtcgaaggat tgagtgatag acaaagtgat ttagaagagg aaataaaggg tcctgctaag
    49081 aaaattgctg ttgatgcaga aaataattta acaaaaccag cattaggatt tataaaaagc
    49141 aaaggattaa gcgaatctga catatatttt aaacaagttg gaaaagaaga atatatattt
    49201 gcgactataa agcaagatgg aaaagaaact tcagaagtac taaaagacat attaccagag
    49261 actataaaat ctgtagtatt cccaaaagct atgcgttggg gaggaaaaaa tcttaaattt
    49321 gcaagaccta taagatggat agttgcattg ttaaatgata aagtattaga gtttgattta
    49381 gaaggaataa tatcttctaa tgtaacaaaa ggtcatagat ttttaggtga aagtacaata
    49441 gaagttgatt ctatagaaga ttattttgaa aaattagaaa aaaattatat agttttagac
    49501 caaaacaaga gaaaagaaat gataagaaaa caatgtatag aagtagcaaa ttctttaggt
    49561 ggagaagttg aatttgatga agatttactt gaagaggtaa cttatttagt tgaatatcca
    49621 actgcttttt atggagaatt tgatgtggat tatataaaac taccaaaaga agttgttata
    49681 actcctatga aacaacatca aagatatttt ccagtactta aagaaggaaa actgttacct
    49741 aactttatag ctgttagaaa tggtgattct tacagaatag acaatgttaa agctggaaat
    49801 gaaaaagttt tagaagcaag acttgcagat gctttatttt tctatagaga agatactaag
    49861 agaaacttag aaagctacat agaaaaatta aaaactgttg tattccaagt aaaactagga
    49921 actatttacg acaaaacttt aagacttgaa aaattaagtg ctgatatact tgatagttta
    49981 ggtttagtag atgaaaaggc agatactata agggctgcta aattatctaa ggcagattta
    50041 gttactggaa tggtaggaga atttgatgaa ttacaaggtt ttatgggaaa agaatacgca
    50101 aaagttggag gagagaatga tgttgtaagt gaagctattt ttgaacatta tcttccaaga
    50161 tttgcaggag atatacttcc taagactaat gcaggggttg ccttatcaat agctgataag
    50221 ttggattcaa tagcaggttt ctttgctata ggaatacaac caacaggttc tcaagaccca
    50281 tatgctttaa gaagacaagc tttaggtata cttagtatac ttatggacag aaaagttgct
    50341 gtggatataa aagtacttat tgaacatgct ttagataatt attctgaatt agattttaat
    50401 aaagaagaag ttgttgagca aataatgaat ttcttcaatg agagagtaaa aaatctattt
    50461 aaagatttag gaataagata tgatgttgtt gatgctgttt taagttctga tataaatgat
    50521 gtttctgata tgcatatgag agctttagag cttaataatt ggctagaaaa agatgaatta
    50581 gtggagatgc ttactgcatt taatagagtt tctactctag ctcaaaaagc agaaagtgat
    50641 aaaattgatg aatctttatt aaaagaagat gctgaaatta gactttacaa tgaatttaaa
    50701 caagtaaagg taaaagttaa tgaattatta aaggataaga aatatggtat tgctttagat
    50761 gaatttgcat ctttaagacc agtagttgat aatttatttg atacagttat ggttatggat
    50821 aatgatgaag ctattaagaa caatagactt gctttactta aacaagtata tgatactatg
    50881 ctagaaattt gtgatttatc taaaattgtt tataaataaa atttaaacta tttataagaa
    50941 ataaggctgt ctcaaaatag agatagtctt atttttcatt gttgagatga tgaaaatata
    51001 tttgatttca tagaaaaaga gtgtctcata aactaaaaag ttcattttga gacacccatg
    51061 ctttttgtaa gtaaatataa taaattatat tttatactta tcagttattt tgtttaaaat
    51121 attatcattt gaatttaccc attcataaaa attgcctgat gatgcgtccg atattttata
    51181 aagcatttca ggaatatcac tagctttaaa atctccaagt gattttccca atctagtctt
    51241 acctatttca aaactgcctc caacaaataa ttcaaatgca tctgtagata taccatcaac
    51301 attcttcttt ttaccacata aacctatgct accaacttga tgaacaccac atgaatttaa
    51361 acatcctgat atatataact taggtgcttt atttaaaatt tcttcattat tttgtaatct
    51421 aaaataatct atgatttcat gtaacatttt ctgactattt tgtatgccca tttgacaaat
    51481 tggaacacct atgcaagata tactttgttc taattgagaa ttacaactaa tagttttact
    51541 tatttctaat attctttttg cttcatttcc atttaaattt aaaatatata atccctctgt
    51601 cattcctagt ctaatcatag gattttcaac attatctaat tctttaagta aagtacttaa
    51661 atcctttgtt aacaaaatac ctccaactgg atgtatatat acagtgtata aaccatcctg
    51721 tttttgaggt attagtctag aatcttgtat atctatttta actccaggtt tagagtaatc
    51781 tatttcttca atattgagtt caagactacc attttctttt tctaatttat agtattcttt
    51841 aaatttttct aagaatagtt cttcaccaag tgattcgacc atatatctaa ccctagctct
    51901 gtttttattt tcataatttc cataatccat aaagaattta atcatacctt caacacaata
    51961 taaggcgtct ttcggcttta taagttcatc aagttctaaa gcaactttgg gttgtttacc
    52021 tagacctcca cctaagaaaa ctctgaaata tggttcatca tttttcaaag tagcaacaaa
    52081 tcctaagtct tgtatactac aatgagcagt atctgtgtaa caattagaat aagaaacttt
    52141 taattttcta ggtaaatggt atgtagtagc atttttgata aaatatttat cagtagcaac
    52201 tgcatatgga gtaacatcaa agacttcact agggtcaact ccagataatg gagataatcc
    52261 gacatttcta ggataattac caccaccacc tctagtgaat atatcatttt ttattccttc
    52321 ttccatgata tcaacaattg aatttataga taaatcgtgt aattgtatag cttgtctagt
    52381 tgttaagtgt atcttatcta agttgtgttt aatagctatt tcatagattt tattaagttg
    52441 atactgagat aaaactccag cagatactct aagtcttatc ataaaagatt ttttgtctct
    52501 ttgagcataa acaccatatc caccagaaaa tcccttatat tgcattttat ttatttctcc
    52561 atttaaaaat tttgaagaat attctttaaa attaggtagt tcatttaaca atatcttttt
    52621 aaaattatcc attatttcac ctcattgtta gtcttgtata aaaatatatc tattgtataa
    52681 tagatatata tattattatc tagtcaggat aaaaatataa cagaataatt ctattaaaag
    52741 taagaataat aaggacatag tgagtatttt gtttttattt tacaaattaa gtataatatt
    52801 tttgttttat aaaatataat taagataaat agataatttt gtaattttaa tatataatat
    52861 ttagtagagt gtgggagtag cacttaatat ttgcaggagg agtttttgta tatgggagaa
    52921 ataataaatg cattagatag agccctagac ataattttgc tgttatatca tgaaaagaga
    52981 gaaatgggaa taactgaaat aagtaaggct atgggagtat ataaaagtac tgtacataga
    53041 actttggtta ctcttgaaaa caaaggtttt gttatacaaa atgcagagaa tggtaagtat
    53101 tggctaggaa taaatttata tgcaataggt atggttgtgg gagaaaaaat gtcattgact
    53161 gaaatagtaa aaccatatac gaaaaaatta aatcaagaat ttaacgaagt agttaatgta
    53221 tctatattag aagaaagagc tcaagattct ccaagaagta tcataataca taaagagtat
    53281 ggttcaaatc aattactttc agtaaatcca tccgttggtt caagtagtga atgttattgt
    53341 tcagcagttg gtaaatgtct tatggcattt aatgattcta tagattttga aaaatataga
    53401 aaaacaccaa tacacaagta tacagaacat acaattgata attgggatga tatgatgctt
    53461 tttttagcaa aaataaaaga acaaggatat gctatagatg atgaagaact agaacatgga
    53521 ctaacttgta ttggagcacc tatcttagat aaaaataata aagctattgc agccataagt
    53581 ttgtcaggtc caactataag aatgagagaa ggggactttg agtataagat taaaagagtt
    53641 atagaaactg caaaaagtat atctgaactt tttagatgat ttttaatctc gataataaaa
    53701 ataggctata tttaaattgt tatttgtaaa tatcagttta aatgtagcct atttttcttt
    53761 aatctaaaat taaatattct taaaatttaa aatgttattg aaatatataa gcgaatgtgt
    53821 tatcttttaa ttaagcaata aaaatagaac aaggttctat actatagaac aaaattgatg
    53881 catatatagt atttatacaa aatataaata ttatatattt aaaattaagt aaaaatatta
    53941 cataaagggg agatttgaga aatggaaaaa gtcaagttga gtgttgaaag atgtaaggga
    54001 tgctatcttt gtattgaagc ttgcaaaaaa gatgcaataa gtctttctga ctacacaaat
    54061 aaaaaaggct ttgtaactat taaagtcgat gaagaaaaat gtattcaatg tggcgcttgt
    54121 tatacaatgt gccctgattt agtatttgag atattataaa ttatttttat tagaggggag
    54181 aaaaggtatg caaagtttat taattaaagg aaatgaagct ttagcagaag gtgctatacg
    54241 tggtggctgc agattttatg caggttatcc aataacacca caatcagaaa tattagaaat
    54301 gatggcatgg agacaaaaag aagttggagg agtatttatc caaggtgaat cagaaatagc
    54361 cagtgtaaat atggttatgg gagcaagtgc acttggagca agagctatga cttcttcatc
    54421 tggtccagga ttttcattaa aacaagaagg tattgcatat tgggtatcag ctggtttgcc
    54481 agcagttgta gcatgtgtgc aaagatatgg tataggtgat ggatttatag gagctggtca
    54541 agataattat tggcaagcag taaaaggcgg tggaaatgga gattatcatt taatcgtgtt
    54601 agcgcctaac agtgttcaag aaaatatgga tatggcatat gaatcttttg aattggctga
    54661 aaaatatatg catccagtac ttatactttc tgatgctaca ataggtcaaa tggtagaacc
    54721 ttgtatagca ccaccaatga aagaatacga tatggataaa gctccatggg ctgcaaaagg
    54781 aacacctgta ggagaaaaaa ataaagtaat aactgatata acatattttg aagaccaaga
    54841 tacatggcaa aaagggtata tagaaaaaat gagaaaagtt caagaagatg aacaaagatg
    54901 ggaagagata gaaatagaag atgctgaagt ggtatttgtg gcatacggta tttcatcaag
    54961 aatagcagaa ggtgcagtta gagatgctag agcaaaagga atgaaattag gtctaataag
    55021 accaaaaaca ttgtggcctt acccaagaaa agcatttaag aaattaagtg attcattgaa
    55081 gggacttgta acagttgaaa tgaacatgat ggcacagatg aaggaagatg taattatagc
    55141 atgtgataat agatttccag tatattcagt agcaacagct caatatatgg cagaagtaaa
    55201 tcaattaata gattttgctc aacagattat agatggaaaa gctaagcaag aggaggtatt
    55261 ttaattatgg ctaagaaagc accagcaata atgatgtgtg aaaatatgtt ttgtggaggc
    55321 tgtggacact ctatagtcga tagaattatt ggagaagtat taacagaaat ggatatatgt
    55381 caagatacaa ttatatgttc agatataggt tgtaatcata tgtttcaatt tagtatgaat
    55441 gtagatgttg tcgttccacc tcatggtaaa atgggtgcag caatggcagc tatgaaaaga
    55501 gttagaccag ataaatatat atttacaata gctggagatg gtggagctta tgcgataggg
    55561 cttggggaaa ctatgtcagc tgctactaga aatgataatg ttatattcct tgttatgaac
    55621 aataatatgt ttgcaatgac aggagggcaa atggctccaa ctactataga aggtcaatat
    55681 acagcttcta ctccagcggg tagaaattac gcagatcatg gttcaacatt tgatgttgta
    55741 aaagttatgg gaaatttaga tatagcatat ctagcaagag gaacaataac taaccctaaa
    55801 gaaataaata caacaaaaag atacatcaaa aaagctctag aaaaacaaaa agaaaagaaa
    55861 ggtttttgct taatagaagt gcttgtacca tgtccaacaa actggggatt aaagccagta
    55921 gatgctatga agagaattga aaatgaactt gttccaaagt atccacttgg agaatttatt
    55981 gattagggag gaaataatat gttgaatgaa attatatgtg ctggttttgg aggacaggga
    56041 gttcttacag caggaaaaat tataatgtat gttgcatata aggcagaaaa gaaaattaca
    56101 tggttcccat cttatggaaa tgagatgagg ggaggaagtg caaattgcaa tgttgttgta
    56161 agtgatgaaa aaattgcaaa tccttgtgta aaaaatccag atatactatt gggaatgaac
    56221 agcgtatcag tagataagta tgaaagttta atgaaaaaaa atggttatat gtttgtcaat
    56281 gaatcactag taagtaagga tagaaagtac agagaagaca ttaatgttat aaaagtacct
    56341 gttactgaaa tagcacaaaa tttaggcaat gaaaaagctg ctaacatttg catgttaggt
    56401 gctatggtaa agagtttgga tttatttaca aaagaagaat ttgaaagtgg tatgtgtgaa
    56461 tactttgaaa atcaaggcaa aggtaaattc aattctaaaa atgtagaagc atttgaagca
    56521 ggatacgact acgaatgaat attttaaata tttaaaataa ttattgaaat acttattttt
    56581 atatgttagt atttaattaa taaaatatag aacgtcgttc tggacaatag gacgaagtct
    56641 ttaggccgga atacaagaac gacgttctgt atagtagaac tagggggtgt taaattgaaa
    56701 gttttagtaa taaatcctgg tggtacatcc acaaaaattt ctgttttcca agatgaaaat
    56761 gaaattttga aaaaaaatat aacccataca agagaagatt taaaaaattt ttctaaagta
    56821 tttgatgaat atgattatag aaaacaactg atagtagata ttttaagtag tgagaatcat
    56881 agtataaatt cttttgacgc agtagttggt cgtggtggac ttatgaaggc tataaaaggt
    56941 ggtacctata ctgtgagtga agaaatgatt gaggatatga gaaatgaaat aaatggggaa
    57001 catgcatcaa atttaggtgc attattagct aaaactatag ctgatgaaat aggagtacaa
    57061 tcatttgtag tagacccagt ttctgtagat gaatttgatg atgtatcgag gattacagga
    57121 atatcagaca tagaaaaagc aagttggtta catgcattaa accataaagc tgtttcaaga
    57181 aaaattgcac aaaaactagg gggaaaatac gaggattata actttattgt agctcattta
    57241 ggttcaggaa tatcgattgt agcacataaa caaggcaaaa tgattgatgg aagtggtggt
    57301 aggtctgatg gtccattttc tccagaaagg agtggtggat tacttgcata tcctcttata
    57361 gaattgtgtt actccggaaa atatactcat gatgaaatgg tcgcaaaagt aagttcgatt
    57421 gggggaatgt acgattattt aggaacaaag gacatgatag aaattgaaaa cagaatcaag
    57481 gagggtgatg aaaaggcaaa acttataatg gatgcattta tttatcaagt agcaaaagaa
    57541 atttctatgt atggagcttc actttcaggg aatgtagata gagttatctt gacaggaggg
    57601 atatctcact cagaaatggt agtcaaagga gtagcagaaa aagtttcata tctagctcca
    57661 atagagatag taccaggaga gatggaaatg gaggcacttg ctctaggagc acttagagta
    57721 cttaatggag aagaaatagc aaaaacttat taatatttag gagaaagagt atgttaaaag
    57781 attttaataa tttattagaa gaggctaaaa agcttgaacc tgttattgta agtgttgctg
    57841 ttgcagaaga caaagaagta ttaaaggctg tgaaatcagc aacagattta aacattataa
    57901 agccaattct tgtaggagac aaggataaaa tagaaagtat atcaagagaa attggattaa
    57961 aaggtcatga aataataggt tgtcaaaatc ccgatgaatc agttaaaaaa gctgtagaga
    58021 tagttaaaaa taaggaagct aaagttctta tgaagggatt agtaaataca agtgtctata
    58081 tgagagctat attaaataaa gaaacgggtc ttagaacagg aagattatta agtttgcttg
    58141 cagtatatga attacctcaa tatcataaac ttctttattg tactgatagt ggaataaatg
    58201 tatcgccaaa cttaaatcaa aaaaaagata taatgacaaa tgcacttctt actatgaaaa
    58261 gtataggcat ggaaaatcct aaaactgcga tacttactgc aaatgaaatg gtagacccta
    58321 agatacaatc tacagtggat gccaacctac ttgtagagat ggaaaaatcg ggagaaatac
    58381 caaagtgtat agtggaaggt ccaatagcat ttgatgtagc atttgatgca catgcagcag
    58441 aacataaagg tattaaaagt aatattgctg gtgatgttga tttattagta tttccaaata
    58501 tagaagcagg gaatatatta ggaaagtcat ggcttagatt taataaagct aagtgggcag
    58561 gtatagtttt aggagcaaca aatccgataa ttttaggttc acgttctgac acagctgaaa
    58621 taaaaataaa ttcaatagca ttagcgtgcc tagcatcaca ataagaaatt attattaagg
    58681 aggagaaagt attatgaaat atgcaaaaag aatggatatg gtaaaagctt cagctataag
    58741 agcttctcaa aagaaaatag ctcagaaggt ggcaagtggt ggaactgtta tatcttttgc
    58801 tgcagggctt cctgacccaa atttatttcc aatagaagag ataagtgaaa taactcaaaa
    58861 agtacttaat gaaaggggaa aatttgccct agcatatgga cctactaaag gtgaagatga
    58921 gttgttagat ctattagtaa aaagaatgaa agaagaagaa aatattgata caaaagcaga
    58981 aaatattata ataactactg ggtcacaaca agggatagca ttaagtgcta tgattttact
    59041 agaaaaagga gatatagtag ttacagaaaa tccaagttac ttaggtgcaa taaatgcatt
    59101 tagaccttat gaatgtgatt ttttaggagt agatactgat gatgaaggaa tggtaacaga
    59161 tgatttagat aaattattat ctgaaaattc taacataaag gtaatctatg taataccaac
    59221 atttcaaaat cctacaggaa aaacatggtc tttacaaaga agaaaagatt ttatggaagt
    59281 cgtgaataag tatgatgtca tagtaataga agataatcct tatggagaaa taagatttac
    59341 accagagcct ctacctacac ttaaatctct tgatactaaa gataaagtat tatacttagg
    59401 ttcattttca aaagtattat gtccagggtt tagagttgct tggatgtgtg gtaatgtaga
    59461 catcatagat aaggcagaac ttttaaaaca aggtatagat ttacagtcaa atcaattttc
    59521 gcaagtacaa gtaatagaat ttttaaagaa atacaactta aatgaacata tagaaaaaat
    59581 aagagcagag tataagaaaa gatgtctgct aatgttagat gctatgaaga aacatttccc
    59641 agaggaagct aaatatacag aaccagatgg aggaatgttt atatgggttg aacttcctga
    59701 aagtataaat gtagatgatt tattggataa agccattgat gctggagttg cttatgtttc
    59761 tggagagtcc ttctttgcaa ataatggtcc aaaaaatact atgagactta actatacaac
    59821 aatgtcagaa gaacaaatcg ttaaaggtgt tgagatactt gcagaagtta taaaaaaaga
    59881 actcaattat gcttgtgaat aaatataaaa agtgagggtt taaatatggg agaaaaagaa
    59941 aacggtataa agtttggaaa gttaatgttt caacttgtta ttatagcaat atcttgggaa
    60001 ttagtttata ttataccatt tatccaatat acattatatg atccaatcct aaaagcatta
    60061 gaatgtacaa acactcaact aggttttcta cttacaattt atgggttagg taatatattt
    60121 ggagctccta taggaggctg gttagcagat agatttgatt atagaaagat aattttaggt
    60181 tctgtatttc ttaatggagt agtatcattt ttatttgcat ttaatatgaa ctatccattt
    60241 gcagttgcta cttgggtagg ttgtgcaatc acatcattag ttatgaacta tccttctatg
    60301 gtaaaaatac ttagagttat aggaaaagat aatcaaggta aagtttatgg attcaatgaa
    60361 gctatggttg gagtttctgg cgtagtaatg ggagcaatat tcttatatat ttatacatgc
    60421 ttttcaactc ctgtattggg tatgagatgg gtaatgatat ctttaggaat attatcaata
    60481 gtgatgtgtc cagtattatg gtttgtaatt aaagatgtag atatcagaga agacaaagaa
    60541 gaagaagcaa gtgaaaaaat gtctgctggc gattttatga cagtactaaa atcaccaaac
    60601 acttggctag taggaataag catattctgt gtatattcat tcacagttac aatgtcttat
    60661 tttacaccat atataacatc agtacttgga ggttctgtgg cattgtctgg ggcattagca
    60721 ataataagac aacatggtct aaaattgttt ggagcaccat ttggaggata ttgtgcagat
    60781 aaagtaaaat cacctacaaa agtactacta ccaatatatg tatttggtat agcagtaata
    60841 attttattcc tagtactacc tgcatctact ccaatgacta tctttatagc tttaacattt
    60901 gtagtaggta tattaggtta catgggaaaa ggtatatact atgcagttca agatgaagta
    60961 aaagttccag ttaaatattc agcaactact ataggtatag ctgcagcttt aggattttca
    61021 ccagatgtat tccaatttgc attaatagga cactggatag atacttatgg caataaaggt
    61081 tacacatata catttatatt ccagatagca attctaataa ttggtatact atcttgttta
    61141 tatatattaa aagttaagaa gaaaagagaa agtaagttag aagcataaat attgtaaaaa
    61201 taccaaatta ttaagtttta tagcaatact taaaatttaa agtgcaacac accaaaatgt
    61261 tatcaagttt tctaggtttt agtataaagt tacccctaaa ggtctggaat tgagtagcaa
    61321 ttatcatcta tttatatatt tacataaacc tatttaattt aaattaaaag aatttcaaaa
    61381 ataggtggta attgtactca attttacacc tcataataaa tacgaaaatt taaaatctaa
    61441 ctatatacta ttcaattata tattaataat tgtatatggt actatttata tagtaacagt
    61501 ttcaggcttt tcattacatg tattccaatt tatattgttt gattatttgt caaatacata
    61561 tcgttaaaaa gtttataaat ttacgtttat atatcgatta atattctttg ttttagagat
    61621 agatacttat agcttgtgga ttaaaaataa tataaataga agatagattt tttagatata
    61681 ttgagttgaa gattatatag aatgttgaaa catagctatt taaattattg tctataaata
    61741 taaaatgaat ggtggtgagt taagtgaccg atttagttaa caccattaat ggaattgttt
    61801 ggtcacctgt attagttgca atgtgtttgt gtataggatt atatttttca atgaaattaa
    61861 gattttttca aataaaagat gtaaaagaaa tgtttactct tttgcttgaa ggagatagct
    61921 ctcatgttgg aatatcttcg ttccaaggtt ttgctacatc tatggcagga aggataggaa
    61981 ctggaaatat agctggtgta gctgtagcga tagcaatggg agggccagga gcattagtgt
    62041 ggatgtgcat aatggctaca ttagggtcgg caactgcatt tgtagaatct actttagctc
    62101 agatatataa agatgagcat gatggacagt acaggggagg tcctccatat tactttgaaa
    62161 aaggtcttgg ttggaaaaaa tgttcaattt tattttcagt ggttgcaatc ataagttata
    62221 cattttttat gcctggcaca caatcaaata ctctatatct agcaatgaat caagcatttg
    62281 gattttcaaa aatgacaata gcagttacat cttctattgc actaggtata ataatttttg
    62341 gaggaattaa aagaattggt ttatttgctg agaaagtagt acctataatg ggtggagcat
    62401 accttattat gacattggta ataatcatag caaatataaa tctagttcca ggagctatta
    62461 tgacaatgtt taaatctgca tttggatttg gttcggtttt tggagggatg ttaggttcag
    62521 caatatcttg gggagtaaaa agaggtatat actcaaatga agcgggagaa ggttctggaa
    62581 cttatggtgc agcagcagca gaagtgagtc acccagccaa acaaggatta gtccaagctt
    62641 tttctgttta tgtagacaca ctattaatat gtatggctac aggtattatg attataataa
    62701 caggaatgta taatgttaca gatgtttaca caacattacc aggtgtagaa ccaggtccac
    62761 aatatgtaca gagtgcaata aatacattac atcctcaatt aggtgatatt tttatagcaa
    62821 tagttttact actatttaca tttacaacat tattagcctt ctcatacatg atggagacca
    62881 acgtatctta cttagtgact agaattaagg cagaaaaaca tgaaaagcac ataattaatg
    62941 tctgtagatt tatgcttata atatgtgcag caatgtgttg ctttatagaa ccacttacaa
    63001 gttgggcact tggtgatata tgtataggtg tattaacata tataaactta gttggtatat
    63061 ttacactaaa aaaaccagct ataaaagcat attacgatta tcaaaggcag aaaaagatgg
    63121 gagtaaaaag gagcgataga acttttaatc ctatagaatt agatataaga aatgctgagt
    63181 tttgggaaaa tagatactta aaagaggata agcatgagtt aaattaaagt tataataaaa
    63241 tataaaaatt actaaataaa tttagtggga ggtaagattg gtatgaaaga aagaataata
    63301 caagtggctg attcaaagaa agaaaaaatt ctagggctat gtcaatcttt atatgatgaa
    63361 ccagaaattg ctttgcaaga atataaatct gctaaaaaaa tatcagaatt tctaagagaa
    63421 gagggttttg atgttgagga aaacttggca ggtatggcta cagcttttaa agcaaccaag
    63481 aaaaatggag atggtcctaa aatagcattt atagcagaat atgatgctct tccaggcaat
    63541 ggacatgcat gtggacatca tttaattgca tctatgggag ttggagctgg aatagctctt
    63601 tcaagtatac tggatactta caagggagaa gtaagtataa ttggaacacc tgctgaagaa
    63661 actggagatg gaaagcctta cttgatagaa catggtgtat ttgatggcta tgatgcagca
    63721 atgatgatac atccaaattc aaaaacttgt gtaacacctg aaattatagc aataggaggt
    63781 ctagatttca tatttactgg aaaagcatct catgcaggag ctaagccata taacggaata
    63841 aatgctttag atgcagtagt actactatat aataatataa atgcactaag acaacaatta
    63901 gtagatggaa cgagaataca tggtattata ctagaagcag gaactgctgc aaatgtaata
    63961 cctgatatgg gaaaagtaag attagagata agggcaaaag aacaaaatta ctttgatgaa
    64021 gtagtagaaa aagtgaaaaa ttgtgctaga ggtgctgcaa tagccactgg atgtgaactt
    64081 gaattttatc actttgaacc aacttgccaa ggattaaatg aaaataaagt tttggtagac
    64141 atatttacaa agattatgga ggaatttgga atatatgaag atgaacaagt aatgttaggt
    64201 tcaacagata tgggaaattt aagtcaaata atgccatgta tacatccttt gatgaagttt
    64261 tctgaaaatg gagaagaact acatacaaaa gaatttttgg aggcttctat aaattcatat
    64321 gcaaaagata gagtaattga tggtataaaa atattagctt taacaggatt taatttattt
    64381 gaaaatccag agttattgaa aaaaatgaag gaagagtaga acttagacta tttttaatta
    64441 tactctttaa aataagaaga gtgtctcaaa atgaactttt tagtttatga gacactcttt
    64501 tttgcatgaa attaaatata tttttattat tttaacaatg aaaaagaggc ttatctgttt
    64561 ttatataacc ttttttaact tgtagcacaa aatatcatga atgattacaa ttcgaatgat
    64621 aaaatcgatt ataagatata taggaggggt acatatggaa actttagaag taagaaatat
    64681 tgaattgagt ggtacaaatt atgagatagg atacagacta ggagaattgg ttgctaatat
    64741 gccagagata atagaaggtc agattaataa atctaatctg gtaagcaaaa aagaagaaaa
    64801 agagatgata gagttgtttg ataaatattg tccaggtctt aatgaagaat tgcaaggttt
    64861 tgctgatgca attcaagtga attgtaatca aattttatat tatactatga cttatcttaa
    64921 accaggttgt agtcaagttg cattaacacc agaattaact gaaaatgggc atgtactatt
    64981 tgcacgtaat ttcgatttct cacacaatat ggaggatttt gttttatgta aaactaaagt
    65041 aaatggaaaa tatgcacata taggtactac cgttatgcaa tttggaagag gtgaaggtat
    65101 gaatgaatgt ggattaggtg taagtcagtc atcatgtggt attcctgtag gcaattcaga
    65161 tggtttgaga aaaccagcaa ttgtaggttt acagttttgg gctgttatta gatatttgtt
    65221 agaaaattgc aaagatgtag atgaagcatt agaatattta aaagatatgc caattgcata
    65281 caatattaat ttattgcttg cagataagtc tggaaacatt gcacttgtgg agactttaga
    65341 tgggaaaaaa gaagtaaata taatcaacag tttagaaagt aagagagaat attttttaca
    65401 ctctactaat catacacata ttgataaact tcataaatta gaaccacaat caatgaaaaa
    65461 ttctattcat agatataaat taattaaaga atatataaat aaggctaaaa aaattggaga
    65521 gaaagagtta atgaatttat tgtctttaaa gtatccaaat ggcttatctt gtaattacta
    65581 taatgatttt tttggaactt taaaaagtat agttatggat ttaaatatag gaaagtttaa
    65641 tattttatgg ggtggactag agaataaatg ggagagctac tatttaaaaa atgacattaa
    65701 atctataaca cagaggataa acataaatat tgaaaaagca ccatctgact tttttgattt
    65761 cattgaataa aaaatgtatt taattattct aaataattag gctatataaa tcaactaaaa
    65821 gtttttattt atcgtattga attagttata atattttagt atagtaaaaa atagtgctat
    65881 tttacatatt taaagagttt aagtagaaga gaggtgccat attggattac ttaaagtatt
    65941 ttgaaaaagc tgtaatgtat attgagaata atttacatga aaatataact gtaaatgatg
    66001 tggcaaaaga aactggatat tcttattatc atttaacacg cttatttaag tctatgtttg
    66061 gtgagagtgt aggaagttat ataaaaaaaa gacgattagt aagtagtaca aaagaacttt
    66121 tatatagtga taaaaaagtg atagatatag ctattggtag tggatttgaa tcttcggaag
    66181 catttagcag agcatttaaa tctatataca aggttagccc tatagaatat agaaaaaata
    66241 gaattgatgt atttgttggt aaaaagaaaa aattagagtt agattttatg agacatcttg
    66301 ttggaaatat aacgattaaa ccagttataa aagaaataga aaaaataaag gtaataggta
    66361 taaaggataa agtaatttta gaaaataatt cattacctga cttgtgggag aaattcagaa
    66421 aagtacatca tattgtccca aatactttgc catcaaagag agtttttgga atctgtgaag
    66481 caatctctga aatacaccta gttagtgaaa gtatggaatt taatgagata ataggacttg
    66541 aagttaatag ttatgatgtg attccaaatt catttgtttc aaaaactatt aaggaaggaa
    66601 agtatgctat atttactcat actggtagct tagataactt agacaaaact tatgaatata
    66661 tatggggtac ttggttttta agttcaaaag aagaactgga tataagagat gattttgaag
    66721 tatatgatga acgattttta ggtcctagta atgtgaactc acaaattgat atttatatac
    66781 ctataaaata atctataaga aaactaaaag ataatttgat taagaaatag atgttcgtaa
    66841 aagagcatct attttttatt tttatcagat ttagataatt ttaataataa aagaaataaa
    66901 aataagttct taaatttcta agaagaattt agaatatagt cgaaataaga attgtatatt
    66961 tctgaaaatt ctaattagta caattttttc ctttataaaa ttgaaaaaag atgtatttat
    67021 ttatcaaaaa tattatatta aaatgatatt aataaaaaaa catttacaat attagcatat
    67081 agtacagaaa aaagatgtaa tttgtaaaat ttatgttaag cagtaaaatt aaaaatttaa
    67141 agatattata gggaggttat aattttatgg aaaacgttat tacaggtcta agtaaaggtg
    67201 cagagtggtt tatcggtttg tttcagaaag ggggagaaca atttataggt cttgtttcag
    67261 gtactcttcc aacgctaatt gtattaatgg tagctattaa ctctttgatt aagataattg
    67321 gtgaggaaag agttaataat tgggcttcta agctaggaaa gaactctttt acaagatata
    67381 ttctacttcc attagtatca gtatttttct tgggaaatcc tatgtgctat acatttggta
    67441 gatttttaaa agaagagcac aaagcaggat tttatgatgc agcagtttca tttgtacatc
    67501 caattacagg tctttttcca catgcaaatg caggagaact ttttgtatgg ttgggaatat
    67561 cagcaggttt gactacttta ggtaaagaaa ctactacact ggcattgtgg tattttatag
    67621 ttggtctaat tgtaattttt ataagaggta tagtaacaga aaaaatgtat gcttttctaa
    67681 caagaaaaaa tagagcaaca aataaggctt aggaggtaga taaaatggag gagaataaaa
    67741 tattaaaaat agaaaaggga acttcaggct ggggaggtcc tttgtatata aaaaaagaag
    67801 ggaatagaaa taagatacta tcaatgactg ctggtggtat ccatgaagta acattaaaaa
    67861 taaaagaact actgggttgt gaaattgtag atggatttaa aactggagtt tctgatgaag
    67921 aagtagcagt agttattatt gattgtggtg gaactgctag atgtggagtt tatccaaaga
    67981 aaaaaatacc tacaataaat gtaaatccag ttggaaaaac aggtccttta gcaaaattta
    68041 taacagaaga atattatgta tcagatgtta atcctaactg tataagtgtt gtagatgggg
    68101 aagatatgcc acaaaaatct caagaaaata aaagcgaaaa taagagttct attagaaagc
    68161 ctgataatta tgatgaggtt aagtcaaaag cacaaggtga atatgctaaa aagaacataa
    68221 tactttctat tggtcaaggg gcagggcaag tagtatcaaa attttatgat gcaggtcgag
    68281 acactatcca aatggtaatg aataacgtaa taccatttat ggcatttgta agtatgctta
    68341 tgggaattat tttagcatct ggactgggag actggattgc aagagttata tctccacttg
    68401 caggtaatat tggtggatta ttgattatat cagtaatttg cacacttcca ttcttatcac
    68461 caatactagg acctggtgca gttatagctc aagtagtagg gactcttgta ggtacacaaa
    68521 ttggtcttgg tgcaatacca gcatatttag cacttccagc tttatttgca ataaatgggc
    68581 aagctggatg tgattttgta ccagtaggat taagtttggg agaagcagaa ccagaaactg
    68641 tagagtatgg tgttccagct ttattttact ctcgtttaat aactggtcca atagctgtta
    68701 ttattgcata tggagttgca gtatttgcat tgagataata tttaatgtca atataaggct
    68761 tggaattaat taataaaatt gtaaatataa atttaagcta gtcaagattt tttgttaatg
    68821 aaagactagc ttttatacta ttaattagct gtaattatcg agttagatta atactaaatt
    68881 ttcctttatt aaagtgaaat tttatgtatt aatttggttt caaatttgta ctagaatgaa
    68941 atcaaggagg agatacaaaa tgagtgtaac tgagaaaatt ccttctattg accgcaagat
    69001 atttaagata ttgattatgt gtagtaaaaa agagtttgtg agtattaatt caatagcaaa
    69061 tgaattgaat gtcacaacta gaagtgtaag gacgtatata aaacaattga ataaagattt
    69121 aggtaatgat atagcagtaa tcaaatatat aaagggtcaa ggctataaac tagaaataaa
    69181 agatgagcag atactcaata agattattga tatgaataga aaaaatgtat tttcattaaa
    69241 ttcaaaagaa gatagagtag agtttatact aaattacttg atagaactag atggatttat
    69301 tacacttgat agtttagctg atgagatgtg tgtaggaaga actactttag taaatgattt
    69361 ccagtatgta gaaaaagttt tggcttcata taatttaaac ttgattaaaa aacagaatac
    69421 a
//
LOCUS       DAEPOG010000002        67926 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEPOG010000002 DAEPOG010000000
VERSION     DAEPOG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.002"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..572)
                     /locus_tag="KR432_000066"
     CDS             complement(<1..572)
                     /locus_tag="KR432_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0638631.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDV"
     gene            complement(670..2046)
                     /locus_tag="KR432_000067"
     CDS             complement(670..2046)
                     /locus_tag="KR432_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0638632.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2046..2702)
                     /locus_tag="KR432_000068"
     CDS             complement(2046..2702)
                     /locus_tag="KR432_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0638633.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(2712..3989)
                     /locus_tag="KR432_000069"
     CDS             complement(2712..3989)
                     /locus_tag="KR432_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0638634.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(4008..4898)
                     /locus_tag="KR432_000070"
     CDS             complement(4008..4898)
                     /locus_tag="KR432_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0638635.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(4891..4986)
                     /locus_tag="KR432_000071"
     CDS             complement(4891..4986)
                     /locus_tag="KR432_000071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0638636.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(5065..6222)
                     /locus_tag="KR432_000072"
     CDS             complement(5065..6222)
                     /locus_tag="KR432_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0638637.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            6556..6906
                     /gene="mobC"
                     /locus_tag="KR432_000073"
     CDS             6556..6906
                     /gene="mobC"
                     /locus_tag="KR432_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBH0638638.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            6912..8243
                     /locus_tag="KR432_000074"
     CDS             6912..8243
                     /locus_tag="KR432_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH0638639.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(8279..9085)
                     /locus_tag="KR432_000075"
     CDS             complement(8279..9085)
                     /locus_tag="KR432_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HBH0638640.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(9075..9536)
                     /locus_tag="KR432_000076"
     CDS             complement(9075..9536)
                     /locus_tag="KR432_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBH0638641.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(9564..10631)
                     /locus_tag="KR432_000077"
     CDS             complement(9564..10631)
                     /locus_tag="KR432_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0638642.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(10628..12250)
                     /locus_tag="KR432_000078"
     CDS             complement(10628..12250)
                     /locus_tag="KR432_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0638643.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(12453..12683)
                     /locus_tag="KR432_000079"
     CDS             complement(12453..12683)
                     /locus_tag="KR432_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0638644.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(12823..13482)
                     /locus_tag="KR432_000080"
     CDS             complement(12823..13482)
                     /locus_tag="KR432_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0638645.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(13525..22254)
                     /locus_tag="KR432_000081"
     CDS             complement(13525..22254)
                     /locus_tag="KR432_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0638646.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(22337..23140)
                     /locus_tag="KR432_000082"
     CDS             complement(22337..23140)
                     /locus_tag="KR432_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBH0638647.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(23225..24931)
                     /locus_tag="KR432_000083"
     CDS             complement(23225..24931)
                     /locus_tag="KR432_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBH0638648.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(25026..26567)
                     /locus_tag="KR432_000084"
     CDS             complement(25026..26567)
                     /locus_tag="KR432_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBH0638649.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(26542..26781)
                     /locus_tag="KR432_000085"
     CDS             complement(26542..26781)
                     /locus_tag="KR432_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0638650.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(26793..28925)
                     /locus_tag="KR432_000086"
     CDS             complement(26793..28925)
                     /locus_tag="KR432_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBH0638651.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(28929..31358)
                     /locus_tag="KR432_000087"
     CDS             complement(28929..31358)
                     /locus_tag="KR432_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0638652.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(31270..31659)
                     /locus_tag="KR432_000088"
     CDS             complement(31270..31659)
                     /locus_tag="KR432_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBH0638653.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(31663..31923)
                     /locus_tag="KR432_000089"
     CDS             complement(31663..31923)
                     /locus_tag="KR432_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0638654.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(31941..32804)
                     /locus_tag="KR432_000090"
     CDS             complement(31941..32804)
                     /locus_tag="KR432_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638655.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(32824..33039)
                     /locus_tag="KR432_000091"
     CDS             complement(32824..33039)
                     /locus_tag="KR432_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBH0638656.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(33043..33354)
                     /locus_tag="KR432_000092"
     CDS             complement(33043..33354)
                     /locus_tag="KR432_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0638657.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(33518..33751)
                     /locus_tag="KR432_000093"
                     /pseudo
     CDS             complement(33518..33751)
                     /locus_tag="KR432_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            33893..34066
                     /locus_tag="KR432_000094"
     CDS             33893..34066
                     /locus_tag="KR432_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0638658.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            34071..34781
                     /locus_tag="KR432_000095"
     CDS             34071..34781
                     /locus_tag="KR432_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0638659.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(35052..35213)
                     /locus_tag="KR432_000096"
                     /pseudo
     CDS             complement(35052..35213)
                     /locus_tag="KR432_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            35824..36009
                     /locus_tag="KR432_000097"
     CDS             35824..36009
                     /locus_tag="KR432_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0638660.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            36026..37009
                     /locus_tag="KR432_000098"
     CDS             36026..37009
                     /locus_tag="KR432_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0638661.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            37483..37920
                     /locus_tag="KR432_000099"
     CDS             37483..37920
                     /locus_tag="KR432_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0638662.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            37904..38146
                     /locus_tag="KR432_000100"
     CDS             37904..38146
                     /locus_tag="KR432_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0638663.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            38605..38778
                     /locus_tag="KR432_000101"
     CDS             38605..38778
                     /locus_tag="KR432_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBH0638664.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            38856..40529
                     /locus_tag="KR432_000102"
     CDS             38856..40529
                     /locus_tag="KR432_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0638665.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            40553..41008
                     /locus_tag="KR432_000103"
     CDS             40553..41008
                     /locus_tag="KR432_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638666.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(41056..42708)
                     /locus_tag="KR432_000104"
     CDS             complement(41056..42708)
                     /locus_tag="KR432_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0638667.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(42781..42948)
                     /locus_tag="KR432_000105"
     CDS             complement(42781..42948)
                     /locus_tag="KR432_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638668.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(43216..44574)
                     /locus_tag="KR432_000106"
     CDS             complement(43216..44574)
                     /locus_tag="KR432_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HBH0638669.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(44534..45220)
                     /locus_tag="KR432_000107"
     CDS             complement(44534..45220)
                     /locus_tag="KR432_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0638670.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(45236..46480)
                     /locus_tag="KR432_000108"
     CDS             complement(45236..46480)
                     /locus_tag="KR432_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HBH0638671.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(46908..47213)
                     /locus_tag="KR432_000109"
     CDS             complement(46908..47213)
                     /locus_tag="KR432_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH0638672.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(47197..47490)
                     /locus_tag="KR432_000110"
     CDS             complement(47197..47490)
                     /locus_tag="KR432_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638673.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            47856..48704
                     /locus_tag="KR432_000111"
     CDS             47856..48704
                     /locus_tag="KR432_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0638674.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(48826..49107)
                     /locus_tag="KR432_000112"
     CDS             complement(48826..49107)
                     /locus_tag="KR432_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638675.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(49121..49498)
                     /locus_tag="KR432_000113"
     CDS             complement(49121..49498)
                     /locus_tag="KR432_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBH0638676.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(49596..51158)
                     /locus_tag="KR432_000114"
                     /pseudo
     CDS             complement(49596..51158)
                     /locus_tag="KR432_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(51155..51640)
                     /locus_tag="KR432_000115"
     CDS             complement(51155..51640)
                     /locus_tag="KR432_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBH0638677.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(51642..52664)
                     /locus_tag="KR432_000116"
     CDS             complement(51642..52664)
                     /locus_tag="KR432_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0638678.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(52750..53031)
                     /locus_tag="KR432_000117"
     CDS             complement(52750..53031)
                     /locus_tag="KR432_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638679.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(53050..54684)
                     /gene="rlmD"
                     /locus_tag="KR432_000118"
     CDS             complement(53050..54684)
                     /gene="rlmD"
                     /locus_tag="KR432_000118"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0638680.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(54859..56619)
                     /gene="pyk"
                     /locus_tag="KR432_000119"
     CDS             complement(54859..56619)
                     /gene="pyk"
                     /locus_tag="KR432_000119"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH0638681.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(56671..57630)
                     /gene="pfkA"
                     /locus_tag="KR432_000120"
     CDS             complement(56671..57630)
                     /gene="pfkA"
                     /locus_tag="KR432_000120"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0638682.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(57839..61408)
                     /locus_tag="KR432_000121"
     CDS             complement(57839..61408)
                     /locus_tag="KR432_000121"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH0638683.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(61565..62512)
                     /gene="whiA"
                     /locus_tag="KR432_000122"
     CDS             complement(61565..62512)
                     /gene="whiA"
                     /locus_tag="KR432_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBH0638684.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(62529..62954)
                     /locus_tag="KR432_000123"
     CDS             complement(62529..62954)
                     /locus_tag="KR432_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0638685.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(63023..64129)
                     /locus_tag="KR432_000124"
     CDS             complement(63023..64129)
                     /locus_tag="KR432_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBH0638686.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(64141..64998)
                     /gene="rapZ"
                     /locus_tag="KR432_000125"
     CDS             complement(64141..64998)
                     /gene="rapZ"
                     /locus_tag="KR432_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH0638687.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(65029..65742)
                     /locus_tag="KR432_000126"
     CDS             complement(65029..65742)
                     /locus_tag="KR432_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0638688.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(65746..66660)
                     /gene="murB"
                     /locus_tag="KR432_000127"
     CDS             complement(65746..66660)
                     /gene="murB"
                     /locus_tag="KR432_000127"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH0638689.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(66933..67703)
                     /locus_tag="KR432_000128"
     CDS             complement(66933..67703)
                     /locus_tag="KR432_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0638690.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT        18887 a        14708 c        10365 g        23966 t
ORIGIN      
        1 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
       61 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
      121 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
      181 accgatagat cattatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
      241 tctattctag cctttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
      301 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
      361 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
      421 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
      481 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
      541 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctctacactt
      601 atattataca gcatattatt tgatatttaa aagccaaaaa atttttggtg ctaaaaagca
      661 ccgaaaattt taattattgt caattaataa ttcttttgga ttccttctta atatattgaa
      721 tgaagaaata atcaatgata caagaagtac taaactcata aatataacta cataaattat
      781 gaatttaggt aatacattta tatctaaact tgataatgtc ttgttaaatc cttcagcttc
      841 tgccccacca cctaattggc tagatgcaga ttgtctagct atttgtttag ctatatcacc
      901 agtaacctta ttcaatatat tattcccaag cttatcagct gtatattgag ctaagaaata
      961 agaaccaacg aatgcgggga ttgatataaa taccatctca attaggaatt gaccaaaaat
     1021 ttctaatttt gatataccta atgatagtaa tactgctatt tctttctttc tagcattcat
     1081 ccataagaat aataataatg aaactacaac tcctgcaaat attaatgagc caacaaataa
     1141 tttatttgaa attgagtaga tacctgatat agattgttgt aatgcagggt aatttgatga
     1201 acttttaatc aaattatatt ctctccaatt tatatctaat ttcccaagat cttttattac
     1261 actatcaaga ttcttatcac cttttacaaa gaatgttgca tcttggtata cagctgtatc
     1321 ttctgtatta ccataaactt tagcagctgt atgaacatca gtgattaatg tattttcgta
     1381 tagttcttgt gctgcactta ctccaccgct attatgccca tcgaatagac ctttaatttc
     1441 tacttctact gtttcatctg cacctttctc attatcagca tcaaataaat tagattttat
     1501 ttttatctta tctccaactt taagattatt tttttcagct aaatctttat gcattaagat
     1561 tttattttta tcttcatttt ctaaatgctt tccttctact aatttatatg cttctgatac
     1621 aaattttgtt tcttttgatg agtcgttaac cccagttaac ataactgttc ttttaaaatt
     1681 cttcgctctt tcaggtgatt gattcgcaag agtctcttga gtttcaataa tatcataatc
     1741 aactaaatct gcaacactgt ttattctttt tacataggag tctatactat ctgtttgaaa
     1801 tatcttttta atatcttcac cttttacatt tcccccacct ctaggtgttc ctggatttac
     1861 ttgtctattt atctccatag aaaaactatt tgttatatta gcaaatgttt cttttgaagc
     1921 tctgtccgta gcatctttaa tagataaact aatgagactt aaagttgaca tagataaaat
     1981 aactaatata ataattaatg attttaagct ttttctagtt acataagcaa atgcattttt
     2041 tatcattatt caacctccaa attcatttta tttacttttt tcaacttttt accacttagt
     2101 tctaaaatga tatccgcaga atctgctact tccttactat gtgttacaac tattacacat
     2161 ttatttctat ctttagctaa tgtctttaat atattaatta tttctccagc agtaacactg
     2221 tctagattac cagtaggctc atcagctagt attattggag catctgatac caatgctcta
     2281 gcaatagcta ctctttgttg ctgtccacca gataatttca taacatttct ttttatttgt
     2341 tttttatcta aacctagttc aaacaagata ctttcatctg ctgatttatt tactaatcta
     2401 atattttcaa ttggtgataa ataatctatt aaattatagt tttgaaatac taaagatata
     2461 ttattttttc tatgattact atatcctttc ttttgtatat cttcattctt aaacaatatt
     2521 tttcctgttt gaggtttatc aagtccagca agtaaggaaa gaagtgtaga ttttcctgtt
     2581 cctgactttc ctactatcgc ataaaacttc ccaagttcaa atttttgatt tactcctgac
     2641 aaaacttttt ctttagaatt tgcataacta tatgctacat tctttatttc taatatatcc
     2701 attattttct cctaacttat ttttgataat atttctttag gcttcttgat taatattaat
     2761 gaagaagcaa atacaactga taaaacaata atacttatta atattaaata actttgtcca
     2821 agtgttgtta tatttaacat aaaactacta ttatttatta aacttccacc ggaaatcatt
     2881 gagtcctctg agttaatgaa tccatctaca attactttta gtaatacatt tcctaaaaat
     2941 aaggaagata ttatacttgg tatcgatatg aatattaact cgaatataaa ttgcattata
     3001 atttgtatct tagatgttcc aatagataaa aatataccta tttcataaat tctttctctt
     3061 aaccatagaa tcaagattaa tgaaagaaca accatcccac ctaacataat agaataagtc
     3121 attattttta ttatatgttt tattccactc actgactcta aagactcttc aaacgcatta
     3181 gaatcttttt caacaaaata ctttgactca tcaattttaa gctctttcaa tttgtttaag
     3241 gctaagtctg tagattctgc actaccagaa tacattaaaa ttttatttgc gattttatta
     3301 ttttctgatt tatttaatat ctcttggctt gttgaataat ctacaaacac catattttca
     3361 ctaaaatcag aagataatcc tgtatatgtt tcctgttttt taccagaaaa gatccctata
     3421 attttaaatt tatgactttt tatttttcca cttttttcaa tatctagtaa ttcaagatca
     3481 acttcatcac ctaattttag attgttttgt ttagcaaatt cttcatgaac aataattgaa
     3541 ttcttatcat tttctcctat atttttgcct tctttaattg taaatactcc actactaaat
     3601 aaaatatttc ttttagtatt atttgtagct tcgagtgaaa caacattctt aaattcgtca
     3661 gataaatctt ctctatttat tctttgttct ccgctaacta ctttagcatc ttttagtttt
     3721 gctaatccat catattgaat tattttttct tcaatttctt ttaatttttc aatatcttta
     3781 aattgattaa cattaaaata tttaccatct ttttttgtta ttgatattga agaattagaa
     3841 ctttcatata aagccttttc tatttcattg cttgatttca ttattgttaa acaagaatac
     3901 aagcaagaaa gaactatagt taatatgata aaaattatta aagttctatt tctctttctt
     3961 gtaatatatg ctatagcatt ttttatcact ttcctaacta ctcctctcta ttttcttact
     4021 ccataactaa ttgcatccac tggacaagca tttttacatc gtccacatct gatacactcc
     4081 agatgattgc aattctcaac tggatctata ttcatttggc aaactttttt acattttcca
     4141 caattaatac acttatcctt atccaatcta tatctaaata ttgaaatagg gttaaaaatt
     4201 gagtaaatag ctccaagtgg acatatatat ttacaaaaag gtctatatat aattatagaa
     4261 agtaacaaag taactattag aatgattcct ttccaataat acaagaaacc tatcgcattt
     4321 ctcatacttt tatttaataa aacaagtggt aatcctcctt ccagcatacc aatcggacaa
     4381 ataagtttac aaaaatacgg agaaccctga cctaaaatat caaccaaaaa taaaggtaat
     4441 attattacaa atacaagtaa tattatgtat ttcaattttc tcaaatgttt atcaaactta
     4501 aatgtttcaa cttttttaaa aaagggaatc ttatgtagca aatcttgaat caatccaaat
     4561 ggacacagcc atccacaaac aaatcttccc accaatgctc ctataaaaaa caaaatccca
     4621 actatataat aactaatctt atattttgaa tttccaatta ctgcctgtag tgatcctatt
     4681 ggacaagaac cttttgctcc cgggcatgaa taacaattca tacctggaac acaaattttc
     4741 tttaaattcc caccgtagat tttaccagtc ttaaaccctt caaagtaaga attagtaatt
     4801 aaaaaccaaa cggattgaaa taaatgccta aaacgatctc ttattttata tttatctttt
     4861 tttgtactac catttataat atttttaatt ctatccaatt ccaatacact ccatacaaat
     4921 tcttatcgcc ttatccaaga caactttagt ttctccatta ctatgaccat agtaaatcat
     4981 cgccatacta ataaagataa ttaataaaga caaggcacgg ctttttaaaa acttattaac
     5041 catataccaa tcaccgcttt acttttattt attttttatt ttttccaatt ctttttcaat
     5101 tacctgcttc cattcctttg aactctttgc cccagaataa gtatcaccta caatatttcc
     5161 attactatca acaaagaaag tctctggcat agcctgtata ccattcaatc taccattaaa
     5221 gtttgtctta tctggcatta agaaaggata tgaagccttt gtcttttcat gtattaactt
     5281 tgacttttca attgcttcct tgttttcacc gttatcatca acagtatctg taacaactcc
     5341 aacgatattt acacccttgc ttttcatttc attctgaacc tcaaccaaat caggaatttc
     5401 ctttacacaa gctgtacacc aagttgcaaa tacatttacc atagtaagat catacttctc
     5461 aaaatcttta cttgtaaaat ccttgccatt tatgtccttt gttgatagtt tacgtaaatc
     5521 tttaaccgat tctttgttaa atgcttctgt attttctaaa tcagtttttt ctgatagcac
     5581 aaaaccattt ttaggacgtt cttttttatc tataatttga atctcagttc tttttaattc
     5641 atccagaaga ttactttcag ctccgctatt tgtgcttaaa tagcaatcgt attttccatc
     5701 acttgaaact cctattttag tatgagtatc acattttgta attttagata ttttttcttc
     5761 tgatgtattt ttttcgaata taccaatagt cccaattctt tcaagctcat tttgccaatt
     5821 tttatatcca tcgcccatct tttctataac tgcattcttt tgttcttctg tcattttttc
     5881 aaatgtcaaa atagcatact ttagttcttt atctatagga ctttgatcat ctaacatcgc
     5941 tatcttttta tcatccatat actttttaaa tttgtctgaa agtttaaatt taagacctaa
     6001 atattcataa acatattctt tttttgtttt taaagtataa tctgatggct taaatgtttc
     6061 atttttatcc ttctcattag ttgacatacc tgcctcttca gaacttatag attgtccttc
     6121 tttaatttta ttgctttcat tacttttttt acacccaact gtacttaagc tcaaacataa
     6181 cgtaagtaat aggcatgctc ctcttgatac tatttttttc ataaattttt cctcctttat
     6241 tcctccttta tttttacaca atagcagttt ataactactt tcaataatat tctacacacc
     6301 ttttatgaaa cgttgatgaa atataatttt ttatttttcc aaactatggt ataataatat
     6361 aaatattatg cttaaagttg ttcttacacg attagtatat gatcgattag tatatgagca
     6421 agcacagtaa gtgtacggac acttacgaaa aaattgatga agcagccctc tgggatatgc
     6481 ttcttttttt atcaattttt aacttgttag ggtataccct aagaccccga aatacattca
     6541 aaggaggatt tacctatggc aaatagaata cgaaatgaaa gactggaaat taaactaact
     6601 gaagaagaaa aggctctttt tgaggagaaa cgaaagctcg caaggtgcag aaatatgagc
     6661 cactttatcc gcaaatgcgt tttggaaaag gaaatatatc aagtggattt agaaccgttt
     6721 agagatttac agggcttact gtccaatgct acaagcaaca tcaatcagat tgcaaagcgt
     6781 gtaaattcca ccggcataat ctataaggac gatataaacg atatgaaaaa gcagattgaa
     6841 tatttctcaa aagagttgtg gcaaatacat tctctgcttc ttaacagaac ttctggaggt
     6901 gattaaatct tatggctata acaaaaatac atcctataaa atcaaccctt aatcttgcca
     6961 tttcatatat tgttaatgga aaaaaaacag atgagcaaat cttagtaagc actcataaat
     7021 gccatcaaga aactgctcat acccagtttt taagaacacg aaatgatgtt ggaacaaacg
     7081 gaactgttct tgccagacac cttattcaat cctttttacc cggagaagca agtcctaaaa
     7141 tggcacatca aatcggtctt gaactgtgta aaaagatatt aaaggacgag tacgaatttg
     7201 tcttatctac ccacatagat aaagggcata tccacaacca tatcatcttc aataatgtga
     7261 atatggtaac aggcaagtgc tatcaatcca acaagaaaag ctaccacaaa atcaggtatc
     7321 aaagcgataa gctatgcaaa gaaaacaacc tttctgtgat tgatgagttt tacgaaagct
     7381 ataagagaaa gtacaaaaca aatggcaagt catggtatga aaatgagcaa tctaagagag
     7441 gtacttcttg gaaaagcaga cttcaatttg acattgatcg tcttataaaa cagtctaagt
     7501 attgggaaga atttctaaaa aaaatggcgg agcttggtta tgaaataaag tatgggaaac
     7561 acatagcctt taaaccaaaa gataagacaa gatttacaag agctaaaacg attggtgagg
     7621 actatacaga agaaagatta aaagagcgta ttacagaaaa tcaatctatt acaactccac
     7681 ctgtcaaaag acgaatcgga aatatcatca atatgaatgc caatgccaaa gtgaaagaga
     7741 gcaaaggcta tgaatattgg gcaaccaaac ataacttaaa tacaatggct gagtcagtta
     7801 tttttatcag agaacatggc attaactcca ttaagcagct tgataaatac attcaaaaaa
     7861 gtgctgaaga aagacaagac ttacaagata aaatgaaaga aattgacaag gatatgcagc
     7921 tactttccga tacaatggag caggttcata ctgttaaaaa atatcgagcc tactacaaag
     7981 aatataaagc aaacccttcc gataaagcat tttttgagga gcataaggct gaaattacac
     8041 actatgaaat agctctttca aagctaaaaa aatcctattc aaagctgccg aattcaaagg
     8101 atattttaga taaacttaat aaactgcaag aaaaaaagaa tacccttatt caagagtatt
     8161 cttctacaaa atctactatg gacgaacttt atcaaatacg aaagaactat ggaatttaca
     8221 tgggtaagga gatggaacga taactctaat attatatagc tttcttattt ttataatatt
     8281 aaattgaaat gcctatcaat gatgcaagca tttttatacc taaagctaaa tcaggaatta
     8341 aagctacgga agtagtgata aaatgcatac tttttaaaca agtagtcaat acttctttct
     8401 tcggatgctt ctgcttaatt tcatttttta tattatctaa attctgatta agtattgatt
     8461 tggagttatc atcctccata cctttaatta agtcttctac attgttaatt gccttgatta
     8521 cttcttcagt attaaattta ttttcttggt cagcgttaat cgtgcttccg tgttccgcaa
     8581 tattgacttg aactccagaa ctattaacat tgatattata tagagcatta ttttcatcgt
     8641 accccatatc ggtagaaata tctttcatat aattttcaac ctcaagtaca aacggataga
     8701 ccattctatc gccaaacgcc tttgccatat cttgatattt attactgcca gcataatacc
     8761 agccaaaagt atatgtaggt agattatttc ctgttataaa ggaaaatgct ctatacagta
     8821 gatacgtcct tttattacta tcattgccaa gattcaaaac tttacgtcca taagaattac
     8881 ttatcgtatt taataagtca tctaagccat caacatcata ttctaaacta tgcaaatact
     8941 cttttatcaa caatgtttgc tctatataat ttatcatttc ttccaaattt gcattctgtt
     9001 ctccataata tgaattcata acatttgaag ctaatatcct aaattttcta ctgatttttc
     9061 taagttcttt tttactaatc tccatctatc atctctccac aaaacgggca ataactacct
     9121 tctaattttt ttatactact tattctagcg gttttgccac aacacaaata tttcttttct
     9181 tcataatctc cagtttttct tcctatcgga agttctgctt cctttttaat ctcttttcca
     9241 cgtttaaact ttatactttt actattccta aaactttttt caagattctt tgaaaaatca
     9301 tttagcattt cagcaacttg attttcaact attcgttctg ctaattcata aatatcatcg
     9361 tctaaataat tctcgtgtgt taaaccacaa ttaggacacc aaatatctat tgtgctttca
     9421 gcctcaatat cttccaccgt taacataaat ttttcactac aacttggaca ttttaattga
     9481 acatatccct cttcatccgc tggaacagaa atttcaaatt ttatatcatt agccatttaa
     9541 atttcatctc ctcacaataa aagttataat ttcaccactg gtagactaca aggtcgtgca
     9601 tctttacgta caataacaac actattctca gcacgtgtaa cagcaacata taatctagcc
     9661 ttactttgtt cttttagaga tgtgttattg ttaataatcc aatcccacat cggttgagtc
     9721 ggatatataa gtactcgttg gaattccaag ccttttgatt ctccaaaatt ataatatgga
     9781 tattcattat tgacggatgt ccttttattt tctctcaact gcattgcatt aaatttctgc
     9841 aaatacttct ctacatctgc tttatcaatt atcataacac catctgattc atcttcagct
     9901 ctacgcacaa aatttgtaga tgttctctct ggatacagac tattcgcaaa tttacatata
     9961 atatcattgt ttctataagt acaattaagt gtcgtttcat caattcttac atctaatttt
    10021 ctgcactcat tttttaaaaa ctcatctatt ttcccacaac tatatttttt atattttttt
    10081 gatgagtgag ttgaatatgt gcattgtcta ggatccccga ccattatcac ttcaattctt
    10141 gaagctatta aacactttaa tatttccaaa tcatacccca ccatatcttg gacttcatca
    10201 acatatatac agtcatacat tccactgatt ctttcaaaaa cttttccatt acatttatca
    10261 tttaactcta caacacactc tgataattta tcagtatata ccctgttatc tttggcaaaa
    10321 tatttgttgg atgttttctt tacgtacgta ttgttctgtc catttacaaa tataattcct
    10381 tcaattcgtt ccttaaatct acttccttga taaggtctaa taccatcttt taacaaaaaa
    10441 gaaaaccatg tagaaactgt aatattatta ggaattacac caactttatt cttaattcgg
    10501 ctaattattt gttgctcatt tgtaatagta tatgttagga ttagtatttt tttatgagta
    10561 ttaagagaat cctctataat ttttcttgtt tttccagatc cagcagcagc tatatataat
    10621 ttcccactca tattagaata ccctccttta tatattgtgg aaattctata gctttatcac
    10681 tttcaaaaat ctttaaagca acttctgttt tatgcccctt catatattta agtaaatcat
    10741 ctggatcttc atatgattta ttcaatatct cgtttaaaat agacatggaa ttcgatctat
    10801 atatacacgg ttccaaagta ttatagttgt aattttccaa agaaccatta tattcatggg
    10861 tactatcatc aaaatatata tttatattct cacaacaatt atctcctaaa taatcgctat
    10921 attttctatt tagtgcatca atatttccat cattatcggt tatcactgac acacgtaatt
    10981 ctagcaattt tgcgatttcc aaaaatctta aaaaagaaat tccaaccgat attatatcta
    11041 ttccatcatt aataggcaat ttaccattat gagagtccat atatgctctt tgaacaatta
    11101 gttcgtccga atcaccttcg actaaaattg ctttatttga taataataat cgtaacgtat
    11161 cataacctgc aattctttta aaaaataaat ttgtttcatt tgataaatca cctagccgta
    11221 gtgtttttcc tcgatttagt aaaattaaat tttcaagtcc taatttattt gatacaaaac
    11281 tactatgcgt agtaactatt atctgttttt cttttactga ctcatttact ttatctagga
    11341 gaatatttaa gtttgaatat gacaaatggc attcaggttc ttctaaaagc acaatctgct
    11401 tattttctat tttatgatca cttaatgcca attgtgtttt aacaatacat tgtgtacctt
    11461 taccaatgtt attatatggt atttcattta attgggttac caagctgctt tcccatgaag
    11521 ttttcgtacc gaaatctaca gaaattgtca attcgttatt tgatgtttcc gttaattttc
    11581 tatttatatt agcaatattc tcatctctag aaaattgttc tttcataagc ctatgcgctt
    11641 gcgatatatt aataatttca ttatcatcta acagattctt tactattctc gatatgtata
    11701 tatctgaccc actggtagga gtatagcttg tcgtatcaat cattgcagac ttaattggaa
    11761 tgctccttgc agttatattt tctctggcaa aagacatcca acttacctca taatattcaa
    11821 ttggcaacga tgttatcttg ttcttacaaa gcattttata ctcattgcta tatttttcat
    11881 caaaactaat tgataaacaa atcccagggg tgtctttagt tttttcagaa tttccatcac
    11941 ctaaaaaact tggcatttct gcatcaaaat acgcttcaat aattatttta ggaggttcta
    12001 cattcactcc ttcgttgact tctctaatat agttttccac tatattctta ttgaaaaggt
    12061 actgagtaag ttcattccta atatttttcc catgataagt tccagttaaa accagattaa
    12121 tagcttccaa aattgtagat ttacctgttc cattattgcc aactaatata ttaatcccat
    12181 tttcaaaatc aactataaac caatctgaaa agcatttaaa attctttatt tttaatttcc
    12241 taattgacat tttatccccc ttctttatct aatataaaaa tcatgatttt agaatataaa
    12301 tattgataac tatataaata cagttatgag tttcctgttt aatcacaatc ataaattttt
    12361 tatgctacat cccttaatat tttttcaagc aataatttaa caataagttt tatgatcctc
    12421 tagctcctga aaataaatct atgactgtaa acttagactc ccattctcct tacatcttca
    12481 ataattacgc actccataat atcttcaata tcacaatcca aagcattaca aattctaagc
    12541 aaaacatccg tagttacatt ctgtccattc ttcattttgt aaaatgtact tttactcact
    12601 tttgcctttt ccatcaattc attatttgtc atatctaaat caatgagttt tttgaacaat
    12661 cttttataac taaaaaccct cattcttatc ctccgttcaa gaaaactata tctagtatat
    12721 tataccacaa aatatacttt tttcataaat atattctctt taatcgaact tgcataaata
    12781 aaaaagagcc ggtgcagtcc taagaccaca ccgaccgtaa atttatctct cagcttcttt
    12841 gcttgcttct ttcttttctt tcggtttttc cttatcttca gcctgatatt ttttgatagc
    12901 tccaagtata gattctttct tttcttcctg tcctttcatc tgttcctttg cctttaacag
    12961 ttttgaagaa agtatcctga tattgctatg ctccttgccg ttatcatcaa tggaagttct
    13021 gatctgccca aagagtttga caaaatctcc ctgcttaaag tcctttggaa tatcactctt
    13081 ttctccataa acggagcagt tgtgatataa cttatttccc tcatcatctt tagatactac
    13141 ggagaagttt gctaccttaa aggtttctcc gttcttattt tccctttcaa ttacatcaac
    13201 ttctccaacg atatttccga caatattgat aagattatcc ttttcttccc gaacataaag
    13261 caggtctttt atctgcccct gtccccttaa atcctcaatc atatagtcaa actcctcatg
    13321 cagcaaatta aggctatcat tgtccatata agcgtcatat agcttatcta aagcactttc
    13381 atcattgata cccttttcca tgcttataat cgctttaaca aagtctttat gattatcact
    13441 taacatatct tcaactctat ctctcattgt tttgtaatcc atgtttttat ctcctttcat
    13501 ggtaaaaggg aggttgccct ccctttatct taaaaattcc tgctctttta ttttttgttt
    13561 ttcttctgtt ttactttctt cctgataggc tcttatctgt cctaagatag acggcttatc
    13621 atctgatttt tgtgtggttt cttctttgct atgaagattg tcctccacat cataagttga
    13681 ctcaaaataa tcactgtcct taaaatcact atcatatcta tccggaatac cgtcattatc
    13741 aaggtctttt gcaaacggat catagaagtt accctcatcg tcaatatcaa gtcccgttgc
    13801 tactcttaaa tcttcagaat caacataaac caaatcacta aatgtagata actcaatttc
    13861 atttttcata tttcttagag cttcattttc tcccttattt tcataatcaa agctctctgt
    13921 tttaataggc gtatcatcaa tgtactgtgt ccaagttttc tcctctaaat taagctcata
    13981 ttgaatacca tgtctttcat ccggtgtatt ggtataggcg ataccgatat gctttaagtc
    14041 aggatataga gtatgaaact catcataact gtgattttct tcatactctc tgttacagaa
    14101 gtcaatgatt gctcttttta catcttccac aagaggatta tcatttttct tttcttccac
    14161 ctctcccata tcgagcagct tatttaactc tgcaagtctt aataccttag ttttcaactc
    14221 atctgccttt tcaaaaggct tttttaattc ctctttggca ttttcaagct gttcttttgt
    14281 acttatgagc ttttcttcaa gcatatttag tttctccggc attttctcaa gggcattatc
    14341 cagtcttgtg atattaccgt ccgcacttgt tccaagctct cctaaatgct ttgcaacacc
    14401 gtttaagctg aaattatgag aattagtaaa gaagttatag cttacctcta aatccatatt
    14461 tctatacttt cctataacct tgctttcatt gatttttacc tttgaaatag cttcaagtaa
    14521 tctttctccg gctaatttct tatcggtaat tttttctccc gcaatggtaa tagaagtaaa
    14581 cttttcttca ccttccgctt taggctctac attggatata tcttttttga cagcttctat
    14641 gagcttttct gttcttgcga tttcttcagg atagtttttt gccaccttat cctctaatct
    14701 gtaacggtta gacttatagt ttgcttcaag cattttaagt tttgtaacct cattatccaa
    14761 atccatcttt tctttaatct ttggatcacc cgttgccaac gctttaattt cggcgtaatt
    14821 taagctgctt tcgtccacat cttctgccac acgaacagga gtcttactgg tcataatctg
    14881 tgaaatgaac ttttgcttat tctctatcgt ctgccaaagg taggcatcaa aggtattctc
    14941 tgtaacataa cgatagatat ttaccttctc attttcattt ccctgcctta caattctacc
    15001 cgcacgctgt tcaagatcag ctggtctcca aggcacatcc aaatcatgaa gtgcaatcag
    15061 tttattttgc acattcgttc cggctcccat cttctgcgtt gaacccatta agatacgaac
    15121 ttcgcctttt cttacctttg caaagagttc atccttttgc ttatcagaat tcgcttcatg
    15181 gataaaggcg atttcttctt tcggtattcc cattgctaca agtttttctc taatatcatc
    15241 atagatatta aattctccat ctccttttgg agtagacata tcggagaaaa gtagctgtgt
    15301 tgacctattt tcttttgtct tatcccaaat agcaaataca ttttttacgc atacatttac
    15361 tttactatca gggttatcag gaagtagggg attgattaaa cgctgatcta aggcaagttt
    15421 cttaccgtca ttggtaatct taagcatatt atcaacatcc ggctctacaa ccctatttct
    15481 gacatcatcc gctctttcag aaaggctctt taggatttcc ttttgttctt cagaaggctc
    15541 agttttaata acttcataat tagcttcagg tgtaggaagg ttaagcatat ccgcagtctg
    15601 aatatctgct acttccttga acatactcat tagttcaggc aagttataga actttgaaaa
    15661 tctcgttttt accctatatc ctgttccttc aggagatagt tcaaaggagc tttgtgtttc
    15721 tccaaaagtg gaagcccaag aatcaaaatg ctctaagtga tttttcttta aactctcata
    15781 ctgaagatac ctttgcattg tataaagctc tgtcattgag ttacttacag gtgttcctgt
    15841 ggcaaaaact atgccttttc ctcctgtcat ttcatccata tatctgcatt tcataaacat
    15901 atcggaggac ttaaaggctt ctgactgccc gatacctgca acatttctca ttttggtata
    15961 gagataaagg ttcttgtaat tatgtgcctc gtcaacaaag agcatatcaa ctcccagttc
    16021 ctcaaaggta attacatcgt ctttcttaaa atcatcattt aatttctcaa gccttgtttc
    16081 cagttttttc tttgtctttt caagctgttt tactgtaaag ttctgatttc tgtcatgctt
    16141 gtattcttct acatagttta cgatttcatt aatctgatct tggatgtgct tttcctgata
    16201 ttccttactc atcgggattt tttcaaactg cgtatgcccg ataacaaccg catcatattc
    16261 ccctgtggca atctttccga taaatctctt tctgttcttc ggttcaaagt cttttttatc
    16321 agccaccata atattagccg acggatatag ctgcataaat tcacgaccga tttgtcctgt
    16381 taagtgattt ggaacaacaa acaaagactt actgcacatt ccaagtcttt tactttccat
    16441 tgcggaagct accatctcaa aggtctttcc actgcctacc acatgagcaa gcaatgtgtt
    16501 tcctccataa agacttcttg ctatggcatt tctttgatga ggtcttaaat caatttctgt
    16561 attcattccc tcaaaagaaa gattgcttcc atcatattct ctgtttcgga ttgagttaaa
    16621 acgctcatta tacagtttta caagacggtt tcttctttcc tgatcgttaa atatccagtt
    16681 cttaaattct tcttttaata gctcctgctt ttgtcctgca agcatggttt cttttttatt
    16741 taagacagaa gttttagaac cgtccggatt tataatctgg tcaaatacct ttgtttcttt
    16801 aagatttagg gcatcttcaa tcagcttata ggcatttacc cttgatgtac caaaggtcat
    16861 ttctgcaagg tcatttcctt tatccctgct ttttccctct acattccatt cacttgtaat
    16921 atttgaaaat ttaaccttaa tatcccatct tgcatatccc ggagttttta aagtttcaaa
    16981 gatgaatttt tcaatatctt taatcggtat ccaagtcgca ccaagacgca cattgatttc
    17041 acttgcttct aattcctttg gaagaacttt ggtaagctct gctttttgat attccaaacg
    17101 attcatttca tagcttatca gctctttttc ttttccattc tcaacaaagc caagatgagg
    17161 aagctctcgt tccgtttgtc ttaactttgc aaggtagctg tctacaatgg caatcttatc
    17221 tctaatattt ccgcttaggt actcatcctt tgttacatag ccgtacttat atgaattact
    17281 gccgtttgca caagcgaaag gcaaatctcc atcttcaagg ttaaaggata atggtctata
    17341 aaagttttgt tcttctctga tgtttagata aatttctcct ctaagttctt ctatcaaagt
    17401 tggtctgtct ttacctgtaa ggctttccat atactcaaaa tccacatagc ctttttcaga
    17461 taccgataag acaagagctt caagggaagt gtccacatgg tctatgactt tggcttttgt
    17521 aatggttctc tttgagaaaa tatccccctt tgccttgacg ttttcttctt catcaagaat
    17581 ttcaatggaa gatacaagag ggaaattgct atcctctttt aaggctctgg tattgcttaa
    17641 attatttata aagccatgct tctttgaaaa gttgtcatag acttcattaa gcttttcttg
    17701 agctttcttt acctcatcat cggaatagtc ctctttctgc ttgtagataa catctttaag
    17761 ggcagtattc agttcaagat agtccttaat cttttcctta ttcttatccg ttatctcttt
    17821 cttgatgaaa agggagtttt ctctgtaata gacttcatca tcaataacgg tataggaaaa
    17881 gtttttgaca tcatccgttg caggaattga agtgatttca tcatctaaaa gctctatttc
    17941 ttcataagtt gatcctttgg atatttcttc acttgctttt gtcagaagct cttttaagtc
    18001 ggcattttct ttcggcaggc acgcaagtgt gtttccaaat cttcctgaaa cttcacgcat
    18061 tgtgccgagt acctgctcag gatgatctac aaaatatttg ttataggtaa gaccattttc
    18121 atcttcagca aggtgaatcc agtcctcatc tctttctctg atactgtccc ttttctttag
    18181 gaatataata tctgaggtta cttctgttcc ggctatgccc ttaaaggtat catttggaag
    18241 tctgattgcc cctaaaaact ctgctcttgc tgcaagatag cgtcttacac tttcatcctt
    18301 tttatccatt gttccacttg atgtgataaa ggcgataata ccgccgtttc tgactttatc
    18361 aatggatttc gcaaagaaat aatcatggat aaggaagtta tttttgttat actccctgtc
    18421 atttacctta tattctccaa agggtatatt gccgataacc gcatcaaaga agttattgga
    18481 aaaggaggtt tcttccagtc ctttaatctg cacttcactt tcagggtata gtagttttcc
    18541 aattcgaccg cttaccgagt caagttctat gccataaaac ttggacttat tcatttcatc
    18601 agggatattg cctataaagt ttccgatgcc cattgatggt tctaaaatgt ttccctgttt
    18661 aaatcccata tccgaaagcg tcttatatat tccgtcaatt acggttttcg gtgtataaaa
    18721 gctcgttaaa gtagattctc ttgcagcttc gtattctgca tgtgataagt tctcatttag
    18781 aaaagccctt gcttctttcc actgaccttc cttgctttca tcaaagactt cggaaagtcc
    18841 gccccagcct acataccttg ctaaaacttc ctgagctgta ctgtcaagct ctctttgtcc
    18901 gctctccact ctgttaagca tggaaattgc ttcaaggtta ttatttaacc tttcgcttgg
    18961 acttaacttt tcagggagcg tttcttctgt aattttaaag ttatgagctt ccgctttcct
    19021 tatttgagtt tcctcagtag tctgctctgg ctgtttatat gtcaggtttt caaataccct
    19081 ctcaaggtcg ctttccagac gataaggaat gacatcgaag cttgttatca tcccgccaaa
    19141 atactcggtg ttgtccttca ccgttaccgt atttaggttc tttctcatat catcaaagcg
    19201 tgttatggta tggtttttgt ccttgtattt aacttcatca ccgacaataa agttcggtct
    19261 ttcaagactt attttattca gcaagtcctc attatctgta aagtctacaa tcggaatttg
    19321 atggtttcca cttcttaccg gatcaagcca caaatcatat cttcctgtga tttcattttt
    19381 agaaatttct ctgactttat attcttcatg attaaaatag acggtatcgc ctgctttaac
    19441 tgcaaactct tctaaaaggc tgtcctcttt ttcattcagt tctacttctt ctctttcttt
    19501 gaggtaatca aataaggtag cttgaactgt gccacgcttt gtagcttctt ctgtttcttc
    19561 aggtgcttca agctctactt tatcttcaaa gctaagttct ccattaaaga caaactgaag
    19621 ctcctgctca tccatttgtt ttataagctc tatatcttta agttctctaa aggtctttgg
    19681 aaaatcctct aaaatgagcc tctgggcatt taattctttc agttcctcaa ggttaaaata
    19741 tccccattca ggctctattc caagaacaag tccaaaggca tcaccgcttt ccctgtcata
    19801 ctccgtcata taccaagtcc aatttgaacg aaaaggaata atatatgctg catgaacctg
    19861 cttatccgct aaatctacat cctcctgtgc gtaaatctct ggcactcttt caagaatttc
    19921 atcaggcatt agatttccag gatcattttt agaataatat tgcggttctt ttacttcttc
    19981 tatctctgcc gtactttctg tttccttttc ctcaaaataa agttttttag aaagcagctc
    20041 atcaatatgc tttgcaacac ttgtccaagt aaggaacaca tcattacaat cattcttctg
    20101 taatttaaaa ccctttgcat cgtgccattc atcacttccc attgcacctg acacaacgtg
    20161 agaatgtcct ccgattccat attcatcttt taggaaattg gctttttcct gcaaagtatg
    20221 attttcttta aaaaacttgg tgattcgttc ttttcctcta tcaacgccgc ttcctcttga
    20281 aaggctttca aggacttcat cttctgtaat aaagagcttt accttcggaa gttctgtcag
    20341 attggtacta tattcctttc gttgcagctc aagttcttgc aacttctgat aaagactatc
    20401 taccttgtga taatgatacc ttaatacatt tctgctttct ttgtatcctt ctaaaaacct
    20461 gctatattct ttgattgttt ctttcagata ttcaggattt tttaatgctt cagataatct
    20521 tttcgtttct tgcggaaagc caccacctct ttcaaaagag ttgaaatatc cttgctctat
    20581 tccttcttcg cttaaatcgt gcgttagata ccataaggat tctgaaattc tatccctttc
    20641 atagtcctgt gcctctaaaa gttctacatt tgtagcaaac tcacctctgt caagaagttc
    20701 atttatccta cttgctgcat cactccaact taaaatttgt gtattatcct ctcttgccga
    20761 tgttccataa gctaaatgaa tacctttatc cgaataccag gaagatactt ctctttcatc
    20821 aatgtaaaat ccgtttccgc ctttaaaggt atctttgagg tattctccca attcttctat
    20881 gctcttgcct tttgaaaact cagcaataac aggaagccta ccgccatcat gatttcctcc
    20941 gttaataagg acggtatcta tatccttctc agttagcgga atagtaaggc ttatctgtcc
    21001 ataagaattt tcgggtaaag aaaaagaagc cttttcagct tctattatcc ctttatccgt
    21061 attgtctttt aaatctccac tacttccttg atggtcattt ctttgagagc tgaaatcatc
    21121 gccccatact gcggattgtt ctcgtcctct atcttccaag ctgctatcaa ctttggcttc
    21181 tctgctctca taaactctac tgcctgtttc tgaatatcca tcaggtgtcc tgtcagcttc
    21241 ttctccttgt atagatccaa aagcatctcg aagtggctct gctcctcgct ctgtgaaagg
    21301 taattcagcc tcatcactgc gtaggtcgga ttcggatact tccttataaa gtccgtctgt
    21361 tcctccaagc tgtttagcgt attctccctg attttttcta ttatctcgtc cgtactctcc
    21421 atttcggaga actcgctcgt tttcatttct ttcctgatca tctcgtcgaa atacattttc
    21481 ttctacctcc tctaattctt ctttaatttt attatatccc gcttcttttc ctattaaaac
    21541 ttctttttga agctcaagct ctttgctttt ttgaatggtt tcatcaataa tctttccgct
    21601 aatatccgat acactttcac caaggctcat taaggatatg ctgtcaaatc tttgaaaatt
    21661 ttctcttaaa agctcataat ccatcggata atctaactta aatcttgaag ctaccgcata
    21721 gcttaccgag tcccttacaa acttggtaaa tgatattcta tcctcatccg ctactctaag
    21781 ttcattcatt aaggtatcta ttttttcatc accgtagagt ctgcttaaag aaaagatgtt
    21841 ttcaagtgtg ctttcacttt cctcatagcc ctcgcttttt atcatttcct ttaagacatc
    21901 ttgatgggct tctttatcaa atctccaaag attgacctcg ttgacatccc tgttttttga
    21961 aactgtctgg cttatatcaa agatatagtc caccttttta tatgtgccgt aatcctctaa
    22021 aattggtatc cctttctgcc ctcgcataac cgttcggtta aagcgttccc tccagtagtc
    22081 gaacttggca caggctgtcg cttcaggatt tttatcatat atacttaatt gacttctaaa
    22141 atcatacctt tgattgtttc cgacaacttt taggagcttt agatattctg cttcattata
    22201 caaaacatct tgttttatta gctccaaaat gttatgaaaa tcatttattc gcatttttac
    22261 ctccttcttt tttcaaacaa aaaaggcggt tagattttac tctaatcacc ttcgctatat
    22321 accaaatatt attactctat ttcaattttt taattgattc actttcagat agtatcttca
    22381 tttgttcaat agctattgca tttaattttt ccaatctttc agattggctc atgccttcat
    22441 ttataaatac cgcattaaga ttctcaagat tagaaaggca gacaagctgt gatatatccg
    22501 cataatctct aatattgcct tttaaatccg gattttcatc acgccactcc ttagctgtta
    22561 ttccaaatag agaaacatta agaatatccg cttcacttgc ataaataaaa ttgatttttt
    22621 cttttgaaag tttatccggt acaagtttgt ttttaatggc atctgtgtga attctataat
    22681 tgatttttgc caaatttctc ttaatatccc atcctaactt cttttgttct tcttccttta
    22741 ggcgttcaaa ttccttgatt aaatataatt taaagtatgg cgatacccaa gaagcaaact
    22801 caaacgctat atctttatga gcataagttc caccatatct tcctgctctc gcaacgatac
    22861 cgatagaatt agttttttcc acccattgct tgaccgataa tataaaacgg ttaaatcctg
    22921 cttcattttt aattccctcg aattcggggg aattaaaatt cgtgttatac atttcttccc
    22981 aaatacctaa aaactctatt gtattcttgt ttctgagcca cttttcaatc aaagcagaac
    23041 cgttctcaat atttctaacc atatccgtta aagaaatata atctctattg tctatggcaa
    23101 taatgctgat ttctgaacct tcaacattta ttttagacat ataatcacct ctcaatcatt
    23161 aaattgatta ctttgcatta tagcattttt agagcatttt ttccacgctg aaattagtgc
    23221 tgtcttactt gcgatatgtc aaaaccgctt cttttatctc attttcaatc gcatctaaaa
    23281 attctttctt agaggattta ccctgagcaa tatcagataa ttccatttcc cactttgctg
    23341 tcgtttctgc cgacttaaag gtatctgcta caatcgttac aaggctgatt cctttatgcg
    23401 ttgcaattag attcttctta tctctttcga caaatctctt aaagattaga ttttcaataa
    23461 ttcctgccct tgttgccgga gtgccaagac cttttctttc cacctctaca cctttttcta
    23521 aggcatcatt tcccgcaatc tccatagact ttaagagcgt atcttcagta aagtgtttcg
    23581 gaggttgggt aaatttttct ttaacctctt tattttcaac gctcaaaaca tcaccaacac
    23641 ttacatcggg caatacagca tcttcactct tttttgattt gtattcttta aggtatttgc
    23701 taaagccttc atccttaatt accttacctg aacttgtaaa ttcaaaaccg tcaaatgaag
    23761 ctacaatctt tgttgtattt tcaataagcg gatagcctac acttgcatga aatttatcgg
    23821 atataaggaa gtaaactttt gcttcactta aaggtaattc agaaacatct tcttttactg
    23881 aactgattgt cggtatgatt gcatgatggt ccgtaacctt tttagagtta aatactacct
    23941 tgatacgctc tgtatcaaag tcatttttac ccaaaatgtt gttgactgta ctcacaatca
    24001 tatcctctgt taaacatctg ctgtctgttc tggggtaagt aatcagtttc ttttcataca
    24061 ggctttgagt atagtcaagt gtctgcttgg cactatatcc aaaatattta ttgcactccc
    24121 tttgaagcgt agttaggtca aaaggtaaat caggctttgt aattttttct ttttgaataa
    24181 catcagtaat ttcaatctta tcgcctacta aacttataag ctgttcagca gcaacttcat
    24241 catcaattct gtctgtggag agtgtaaatc cattcataga aagctctact gtgtaatatt
    24301 tttctttctg ataatttgct atctcatcat ctctttttac aatcatggca agggtaggtg
    24361 tctgcactct gccaacactg taattttgct gatacagaca agagtagagc ctactaatat
    24421 tcattccgac taaccaatcc gcaatagctc ttgcctgtgc cgattcaaag agattatcat
    24481 agtcttttcc gtctgttagg ttatcaaaac cctctttaat ggcactatct tccattgaag
    24541 aaatccaaag gcgtttcatc ttctttttac aattcacttg attatataca agtctaaaaa
    24601 tactttctcc ctcacgccct gcatcgcacg cattgataac cgtatcaacc tccttatcat
    24661 tcatcagctt tttaaggatg ttaaactgct tcttggttgc ctttgctact tcatacttgt
    24721 agtcgctcgg aataatcggt aaatcagcca tattccactt ggcgtacttt tcatcataag
    24781 catccggatt tgccatttga attaggtgtc caacgcacca gctcacccta tatccgtttc
    24841 cttcataata tccgtctttc tttttatttg ctccaataac ctttgcaatg gagattgcaa
    24901 cactcggctt ttctgcaata actaacttca tctgtgtttt cctcctgtta taaatcaaaa
    24961 tagggcgaaa gatgttactc tcccgcccta actttgcacc caaaataata tgtttttagg
    25021 tgcgtttatt cttcatcttc tacttcatct gcatcattta tttcttcctc gctttcagat
    25081 tcagaaaaga aatcatcatc ttcctcctct aaggcttcaa gttctttatc ttctttcttt
    25141 ttcacaacct taaaatagta gcctgctcct aatgcaccac ctactacaag aagcaaaata
    25201 atataggttc ctaaattact cttttcgtcc ttcttctcag gctttacttc ttccttaaca
    25261 ggttcttctt ttactacttc ctgctttggt gcttcttttt tctccaccat gttaagcaaa
    25321 tcatcttcag atacttccgt aagaagcatt acattctcac tatcttcatc atgattgatg
    25381 attaaatgaa aagtcttacc gtttttcgtt tgaaaggtaa taaactgcct tgcatctgcc
    25441 gaatactgat ccgtttcttt gttatcgctg ctatctccat gatgaatcgg atactcctta
    25501 tttgcattat ctttattctc cgtaacggat gctctcgcct ttgacggaga tgaagctact
    25561 cccttgttgg tgtttacact cttacttgca gaccccattg atgaatcctg actgttattc
    25621 gcaggagctt ttgccgtcaa cttattggga tagcgaactt ccttttcttt ctcctttact
    25681 tccgttttgc ctttaccggt atcctttgta gtattacctg agcttaccga atccgatgag
    25741 cctttaccgg tactacttac agaagtctgt ggtgtttgag gagaaatacc tgaacttgtc
    25801 ttaattccgg aaataggact aatcggtgta actgattgtg atgttacagg agctttattt
    25861 gtttcagttg cctttttctc aagctctttc accttttccg tcagcttatc tatttccttt
    25921 ttcatatctt ctgataagtc cttatttccc ttatctttct tcattttttc tttcaggcta
    25981 tcaatttcat cttcaaggtc tttaatttta ttcttttgct tatcacttaa tttgtctttg
    26041 tccttaagtt cgcaatttaa cttatcaagt ttatcctgaa gctcttttgc ctctttttct
    26101 atttttgaaa tatcgtcttt agaaagctct gtctgcgttc ccttatcttc tgtctgagtg
    26161 cattcatcct gctttggctt ttcttcttcc gtctgcgttt ccttatcctt aacctccatc
    26221 tttgctacct tacggataat ctcatctttt ccatctccct ttacctcata aaacagaaat
    26281 tcatcaatga atttcatatc atctgggagc attggaagat ctccgctatc aataagttgt
    26341 cctttctcag cctttatctt ttcttcctta aagacctttt catcttcaaa gatatacctt
    26401 acatttactt caacttccgt ttgaacagct tcgttttttg ttggagcttc agatttttgc
    26461 tcctgtgcat aaacaaccgt ccaaagacct aaaatacaac taatacttac aattaccgca
    26521 agtgctaccg tccaaaactt attattcttt ttcaaactgg ttttcatttg ttttctcctt
    26581 tttcatttct gctttttgtc tgtttacctt tgctatcaaa tcgctgattg tgatgttgtt
    26641 ctttctgcaa atggcaatga tttcttcatt ttcaagctct tcttcacgaa tgaataaagg
    26701 ctccaattct tcatcaatca gagccttttt atcctttaac ttctttattc tgttttttac
    26761 tgttgtaagt tcctttttca aattgttacc tcctatttct ttacatttgg cggaaaaccg
    26821 aatcctacgg gatggtgctt acaaaatgtt gctatccaat catctaaggt agttactctt
    26881 attcgaccga tgttatcaat cacttttcca tctccaatgt aaatacctac atgaccatag
    26941 gtaagccctg cactgcttcc gctactgctg ctttcaactg ccacaagcat tcccactttt
    27001 agctttgacc tgtctgatgt aaaggtatag tttcggtaca tatcacaggc attcccgcct
    27061 atatatccaa gtcctgcatt ttgatagacc tgtgataccc acatggcaca ccagcctgca
    27121 cccggagatg gcgtaatgta tgcagcatta acaatcttct tttgtacttc cgtagatgcc
    27181 tcatactctt ttccaccgcc gatacctcca tttgcactga taaggtcaga attaccaaag
    27241 gcttctctca tattgccttg tgcaaggaac aaggcttcat agtgctttag attatcggga
    27301 tagtttgcaa agacctttcg gataatactg tccatctctt ttttatgaag cgttacgatg
    27361 agttttttat actcataagg ttcttcgtgg ctttcggtat attcattgcc atcttcatcg
    27421 gtataggttt ccgtaaccgt cctgtatctg atttcaattt cttccctata ctcaaggtca
    27481 tacatggact caaataattc ttttaatatg gattctactt ccgatacact ctttacctct
    27541 ccgcaccttg atgtaatgta ggataaaagc tcatggacat tatgaccgat gtattctgta
    27601 ttatttaaga tatattcatc atatccagga taattttctt tcacatagtc aacttcactt
    27661 tgaagctcac cctccatatc tgaaaatttc tgattgattt cacttaggac atttggcttt
    27721 gataaatagc ttgttgtaag cacagagctt gtagagttca taaagcctgt cattcccgtt
    27781 cctgcaaagt taatcacaaa agtccctaag ataatgatac ctatgaaaat aagcattagt
    27841 ccttttgctt ttcggataat catatccttt gagcttttca gtgttccgat aagtccctct
    27901 ttaattcgat ctctaagcct tgacttattc tcacgagaaa ttgctgcctt tacctgattt
    27961 ttcttttgaa atcttttata tgcacttgct cttttatact catccgtctt tttcagttcc
    28021 tttttggcat cacgaaattc caactttgac ttacgctttc tgattttata gtccttattt
    28081 gtaaggtcat agcctctttt agctcttttc ttatcagagt acttctttat gccatgaatg
    28141 agcttggagc ttgtatctgc tgtcttttct cctgcttcca ctccttgatt ttcatcactt
    28201 ccatgagaca agtaatctct tacagtttca cttcctttag caagtcctga aagggcagat
    28261 acctttacca tgtctttttt tagctttttc ttttggtctt ttgataggct gctataaact
    28321 tttccgctga cagcatcttt tcccgccttt ttatcatcag ccttatttcc ctttggtgca
    28381 tcgtcctttt tccttgtata aaggctttca gaatagttct tcctcttgta cctgcttttt
    28441 tgagtcttat gatttttaaa ggaattttct aatgattttt ctttatggga aagattatcc
    28501 tccacatcat aggtcgattc aaaatagttg ctgtctctaa agtcattatc gtatctgtct
    28561 ataatcccgt cattatcaag gtcttttcct aaaggatcat agatttttcc atccttaacc
    28621 tccgtaatat aatctgatct tcccgtttca attgcggaat ttacgtctcc cttatctgaa
    28681 gttctatatt tttcgtttct cttggatgtt ccataagact tttcattatc agcacttacc
    28741 ttacttgttt tttcatgcac tttatcctga aaccgttctt tctcatgaac gattttacct
    28801 ctgtaatcat cgttatgttt tagcttactt tcttcaggta tcgtatctgt tttactatga
    28861 aacatctctc tttcaaggct tgccttatgc ctttccttaa aatccttttt cagcttcttt
    28921 cccataggct acctcacttc ttccggcttg gtagtcatct tctgatataa aattgtatct
    28981 ttcggaaact tatcaatgaa aggcacaatg gtatttccaa agaataagag tccctcacct
    29041 tcatttgaat tagtaacata tctgagctgt ggaagtgaga ttttaagttt tcttgcaagt
    29101 atctccctat ctcctgatgc ttgattaagc attaagacaa agtctgtatt gtcaaagata
    29161 ttttctatct ccttactcat aagtaggtct ttgacattct gcgtaatacc tgttggaata
    29221 cctccccatt tacgaaatct tttccaaatc tctacggaat aggatgctgt ctgctcatct
    29281 tttaaaagca aatggaactc gtcgatatag taccttgtag ccttgtttcc tctgttttgt
    29341 gacaccttat tccacacctg atcctgaata acaagcatac cgattttctt taattgactt
    29401 ccaagctcct taatatcaaa acaaaggagc ttcttattta aatccacatt tgacctgtga
    29461 ttaaagacat tgagagaccc cgatacatag atttccatct ctgttgccag cttcttaccg
    29521 accttttctt cctgcccttt taacatatcg tataggtctt gaagtatcgg catattgtca
    29581 ggagtaggat gttcaaaata cttttcatag attttaggaa ggcacctatc tataacggac
    29641 ttttcttctg ctgtaagacc actaccgcct actacaagct caagcatact catgataaag
    29701 tttgccttgt ctttcagcgg tgcatcccca tcaccgtagt tcatgtttat atcaagagga
    29761 ttcaggtaat ccttcgactt actgctgacc ttaatgactt ctcccttaaa ctgccttacg
    29821 aggtttccat actctccttc cggatcgcag ataatcacat catcatctgt tactaaaatc
    29881 gcatttgcca tttctctttt ggcactaaag gacttaccgc tgcccggagt tccgaggatt
    29941 agaccgtttg ggttctttaa tttctttcta tctgccataa tcaggttatg gcttagggca
    30001 tttagtccgt agtaaagact attacttgaa ttgataaaaa gctcctctgt ggtaaatggc
    30061 ataaatactg ccgttgatga gcttgtcaat cctctatcta tttcaatctt atttacacca
    30121 agaggcagca cgctcactaa accttgttcc tgagaatggt caagtctttt cagcttacag
    30181 ttatgcttat ttgcaatgga gcttatttga gcaattgtgt tatcgagctt ttgcactgtc
    30241 cttgcaaagt tcataaagac gatacttacc acaaacattc gctcatctct tgtctgcaag
    30301 tcttttaaga ggctctttac atcctcacca taggtaatta agtccgatgg aagaatgtcc
    30361 atatcataac cgcttcttac cgccttctta ttttcctcaa ttttcatctt atcaatatca
    30421 gtgttttttc gttttaccat cttaatggct tccgattggt cgattgccct aatatgaaag
    30481 gaaatattga tgttatcgtc aatatccaaa aactcggata acatcctatc tgaaagctca
    30541 ctggcaagga tttgaaaatg acttgttgct ccgatgaatt tgccgaactt aaagtatcta
    30601 ctcggtgtaa agttaaactc atcaggcaca atatatgtct ttgtgctttc ccttttcttt
    30661 aggtctttgt aggaaaattt aaatgtttta tttggattta aaacatcatg aagtatcttt
    30721 agcctttctt ctccgtttag gctttctgct cttactccca tacttttaag actcgataat
    30781 atatctatct ccagtctttc aagttttgat gttgcctgtt ctaaattatc cgcttccact
    30841 gtaaaggtta catactttga ttttttcaat ccgttatttc cctttacaat ctggctttta
    30901 agcatctctc taaattcaaa gcgtatatcg tcgaaaccgt cctttttatc cggtatctga
    30961 attgccgact tcatttcttc gtttcgtccg agctgattga tatatgaaaa ctcaatgctg
    31021 atacttggat caaaagagtt taggaagttt gcaaattgat taaaaatcaa gtccctatct
    31081 tcatctaagg caagctgata gttaatgtcc tgaaaggcta tacttttact gaagtgcttt
    31141 tcatcaagct ggcatattcc gctttttaaa agccttagat agggaatggt atcttctacc
    31201 gtatatcttt tcggttcttt cttaaagata aggtcaagga ttcctccctt atcttttttt
    31261 gacttgcaat tacttttctt taagtcccgc ttttgacttc tcaatgcctt ttcgttttgc
    31321 atgagtttta tttgctgaat ttttcttttc ttgttcaagg taaacctcct ttctcactct
    31381 cttttgcggt tgataaaact tatgaagata aatgtactta aagtatttct caaatgtcag
    31441 tccgtctttt tcaaatagtg tgatgaaaaa gataggaagc gtggatacga taaggaatat
    31501 gacagctatg tcatttggca caaacttcct cataaataaa tagactggta ttccaaccag
    31561 tcctgcaagt gtgaagccta tgagctgcct tttcgttagg ttaaaagcaa cctttgtctt
    31621 taccctattt aagtctttag ggattggtac atatgccata tcttacctcc catcttcttt
    31681 accctttgac agctcctcaa agtgttcata tacagagccg atttcttcct gaatagtcgc
    31741 aagctgttcg tctgtgttct tgttatatct ttgctgtatt aggtaaaaat cgttatggca
    31801 gcgaccaatg tcctctgttt tttcttccaa ttcttccacc ttattatgaa gcctgatcct
    31861 atcaattatc gccataatac ctgctccaat tactactata aatactgttt ttcttttgtt
    31921 catattaccc ttcctttctt ttagtgtgca tttaatacgc ttttggccag tgttccgctc
    31981 tttaacatca ttaaccctag caaaaccgca tatccaagta tcgtaaaggt acttgtgtgt
    32041 atatctgtta tctgtattgt cttaactaat actgcgtata ttccaagaca aaccattaaa
    32101 aagagtcctt gtaaacctat ggcaaacaat cctttgatat agtttgttcc aatctgtccc
    32161 cactctttgt ttcccattgt ggcaaatgga atggctgaaa tggatgagta aacataaatc
    32221 tcaaacattc ttccgtaaac cacaagcata atcacaattg aaatgacctg tattgccacc
    32281 tttataagcg aggtttcaaa gagtatcatg acaagctctc caagaccttt atcctttaat
    32341 ccatctacca tttttactat ctgatctccg gagataacgg cagaagtatt gattacccct
    32401 gccgctttat ttaccatgtg ctgtgccaca tcaaagaccg ccattgaaaa ctcaaatgca
    32461 tgtgatacta accatacagc aatccacatc ttgatgatgt acttgaaaaa ttcaaaagta
    32521 tctgtatcgt gcatattgtt cttttgcatt accatattga taagctcgat acaaaggact
    32581 gctgtgatga ttagccctgc tatgggaata atgacggaat cgttaatgct tttaataaag
    32641 gcaaatacat ctccgttcca ccccatagga gtttttccta catctgttgc aactgcacca
    32701 actttatcgt tgatgtcaag aaacatggac tctaagtttg cttgaatacc gcccagtaga
    32761 agctctttaa agaattcttc tatcttgtcg aatattccaa acatctaagc tctccttttc
    32821 ttattacttg agtacatttg caagaagcgg aatcagctta agtccgataa ggacaatgcc
    32881 tcctcccgcc ataagctgct tgatgccctg agatttcgct cccggattat cattaccata
    32941 tccttccata aggttaataa ctccccatgc tccaagtcca gcacctactg ccataactaa
    33001 aatctttaat acatttactg cctgtgcgaa aaattccata atttattcct cctcattttc
    33061 ttctttcttc tcaatcttgt tatatgactt cactacaaag tttttgtaag tctttccctc
    33121 tttttcacgc ttcttaaagt agccgaatac atgaatcaaa tcgccttttt caaagtcctc
    33181 tgctttctct gctttttctc cgtaggctgc acaattgatg tactccttgc cctttccata
    33241 tttttttaca agcgtaaagt ttacaacttc tacttcttca ccctcttttt caaaacttga
    33301 gaaagtaggt tctgccacta agttggcgtt gatgttaatc atttcttgtt tcatcttttt
    33361 aaatttctcc ttttcatttc aataaaaaaa acgactggag tttttctttt ccaatcgctg
    33421 atactcttac tatttagttt tcgtttaagt gggatttctg atccttatca tcttgcctcc
    33481 tgattttgaa taaaaaaaca gcaaatcttt ttaagactta ctgcattctt gtaataaccg
    33541 tctctctatt taattttgct tttccttttc gcttcatata gctttctatg tcaaacaaat
    33601 tcttcttgtc ataatcctca agcagcttat aattcttgtg ctttgtaata tcgtatttat
    33661 ctgaaagaaa aggtctgaca cctctaagct gaaatataca ttttccaccg tccattacag
    33721 taatctcatc ttgactcatc agttctttgc cacaatgtca agtgatgaat taaaaaaagc
    33781 tatcaaagaa atccttcttc gatagcagcg tattcaacat ctattcacat ttcctgcctt
    33841 gcgttcttcg agctgatgaa atataataag aaatgttgtc ggaggtgcga atatggacta
    33901 catgacgctc aaagaggcaa gcgaaaaatg gggcgtttcg tcccgacaga taaattatta
    33961 ctgcaccgat ggacgcatcc ccggtgctgt caaaatggcc ggtgtctggc tgatccccaa
    34021 ggaggcagag aagccaaagg acagacggta taacgaaagc aggtgataca gtgctgcata
    34081 tagcaatttg tgatgatgaa aaagactttg ttcagtattt gaccgattta ctgaaccaat
    34141 acagcaagga aacaggacgg gacataaaga ttaccccgta ctatgacggt atggagctga
    34201 ttgaaaaata cgatccaacc atagacctga ttttccttga tattcaaatg aaggtggtgg
    34261 acggacttca tgctgccgag cgtatccggc aaatggacga aaccgtgggc attatttttc
    34321 tcactactct tacacagtat gggcttgagg gatataaata ccatgcgacg aactacatca
    34381 ttaagcccat gaaatatgcc cggctcaaat cagaaatgga caaattcttg gagcgcagcc
    34441 agaaagaaga taccccctct ctggtgatta ccaacgatac cggcaaatat aaggtgccgc
    34501 taaaatcaat tcgatatgtt gaaacatata accgcaatct gatgttccat accgagcagg
    34561 aaaatattat ctgctataag agcatgaaag aagtggaacg ggagctttgc gacaaagact
    34621 ttgtgcgctg ccataccagc tacattgtca atctgttcta tgtgaaaggc atcaagaagc
    34681 tggacattga attgattaca ggcgagatca ttcccatcag ccagccgaaa cggaaagaat
    34741 ttatggaacg gctgacggac tattgggggg atatgctgta atgtggttat atcaagtgct
    34801 cgacataaca gcaacgctga cagaatcttt gggactgtat ttattcagta tttgtttttg
    34861 caaagaacca cggtatcgtg cttctgtaaa tagatggctt ataacaggta taagtttttt
    34921 gtgtgcgtat acattgacat ggttttctga acttggcgca ctcaaagcgc cttttcttct
    34981 tacgatttat cttgtccttt tgaaagtttg ttatagagat tctgtctatc aatgtgctgt
    35041 tgctgaagaa ctgaattata ttgcaacttg ttctcgatat tcgcaaggta gcgcggggaa
    35101 atatataact cgtcgcttac ctttttgcgg ctctcgccgt attcatttct cgcggctttt
    35161 atagctgccc cgaaagcctt aaagtcgtac aatggtacgg gtctttttgc cattttcatt
    35221 caccctgtta cattttacag ttcacctttc tttttggata tgtccacaca attcatatca
    35281 ttaggttaaa tacttcctgt tgtgtattga cagtagactg attttctgat aaaataaaat
    35341 ttatgtaaaa ggaggcggcg tttatgttgc atagcatgcg tattagataa gcattgaaaa
    35401 aaaggatttt cccattttca acatgggctt ttttgtcatg cttattttgc ggatgctata
    35461 caaaaaacta acgacgtata gatatagtat agacatagtg caagctatgc cttagttttg
    35521 ttgtactgct ctgtgcattt tttgggtagg gaaaccaccg gctgtgccgg tggaggtacc
    35581 ccgtaaaaaa gctttagctt caagcatcga gcgaagcgag ctgctaacag caaattatca
    35641 gtgaagaaag acttgcatta gaacagcggc tgcccgttta cgggctgcgg ggcaaatgat
    35701 gcgggtgaaa taaaattcag tgcgtcttga gacgcatgcc ggtaataggc ccttataggg
    35761 ccaactcaag ccaccggctt agccggtggt tatgacttgt gtaaaaagga cgagtgaaat
    35821 ataatgctta aaaaatattg gataaaatgt ccgatttgta acggaaagac gagagttcaa
    35881 gtatttcata atactgtatt aaaagatttt cctcttttct gccctaaatg caaattgacg
    35941 catatcattg atgtagaaaa attagagatt gtaatcaaaa atacagaaaa acaaactttt
    36001 attatttaga agaaaggata taaaaatgaa acgtttacct aaatatacgc ctgcggaagt
    36061 acggaatgat ccatacggat ttacttacaa agaaatgtcg gaagttattg gcgagaatga
    36121 agcaaaagcc ttatatgaag aattatataa gcaattacca cgcaaaaaaa atctatcaat
    36181 gttggtaaaa aatatttgca aaagcagtga tactgaaaag tatgtttacg aactgaaaga
    36241 caacaaatac attgaaacgg tttttatcaa gcggcgagat ggtggaactg tttgcgtgag
    36301 cacacaagtc ggttgtcctg ttggttgtat tttttgtgag tccgggcgaa atggctttgt
    36361 tcgtaatcta acatcgtcag aaatcgtaca acagattata ttgttgcgtc gaaaagtaaa
    36421 ccgtatcgtt tttatgggta tgggagagcc tttattcaat tatgacaact tgataaaagc
    36481 aatccatatt ctccgagata gatatgggct caactttcca accgacggca ttaccatatc
    36541 aacagttggt ccggtcgatc aattaaaaaa attgcgcgag gaacatctta aaattcagtt
    36601 gacaatatct ttacacgcag caacacaatc tgcaagaaat cgtattattc ctcacatgcg
    36661 catatatgct attgaagatg ttgttaagca agccttatcc tattctgaaa ggcataatcg
    36721 caaaattgtc tttgcgtatt tgcttttacc gggtataaat gaccggccct cagatgtaag
    36781 acaacttgca aaatggtttc ggggcaaaaa agttatgatt aacgtgttac aatacaaccc
    36841 aacaagcaat tcaagaatta aagcaccaca gaaacgggaa atagttgcat tcaaacatca
    36901 attagagcaa gcaggacttg aagttactat gagagtttct catggcagag agattaacgc
    36961 ggcttgtgga cagttagcta acacatataa taaattcaaa aaaaaatgat taaaagtgcc
    37021 agacgcatag acgcagagcc gataacgcat taggtcaaaa aacctatgtg ttatcggctt
    37081 ttttatttaa tattgcgcaa aagccgctgt tccctattat agaatggatt gcgggtagtg
    37141 tattaaagtc gccctttttt aaggatacgg cggtatcggg gaggtatcgc cgtgtccatt
    37201 tttatttact gtaacccgcc taatgctttg cggtcaaaaa aagctatgct ccgcaagcgg
    37261 gatattccgg ctatgaatgt gaactgtcaa tacatttagc tactgcttac atttcctttg
    37321 cgcccgtccg cgtcttgcgg acgggcgcat tttgcttttt tcaacttttt tctgaaaagc
    37381 gcactcaaga gccatctccc gaacgggtac ggagcgaaag gggcagagag gacaagccct
    37441 taactcccgt cctctactcc acgcgggaag gaggtgaact ctatggagct atcttcttcc
    37501 gacaaggaaa gaatacaaca tcagtacgac gcattagcaa agaaaacttt ggtcggcgaa
    37561 gcgaaaagcc accgccgcac tcttgcgaaa cgcgcagcac gcgaagttac tttttcggat
    37621 ttgagcgaaa gcgaactcgc gcagcttttc acaacggacg aatacgaaag cgattatttc
    37681 cgttttcaag tgtccggctt tgatgtactc gtcaaaaatg aactgcttgc cgaagccctt
    37741 aacgctttgc ccgaaaggaa acgcgacatt atccttttgt cctacttctt ggatatgagc
    37801 gacgcggaaa ttggcgaact gctgaatgtt gtacgcacga cggttttccg gcacaggaaa
    37861 tccgcgcttg cgaaaatcaa acagtatttg gagggaaaag cagatgatga ataccgttag
    37921 gaagtctgaa aatctgttgc cgttccctgt catttccgca gcggcaaacg gcgacacaac
    37981 cgccatgtgc gcgatcttga agcactacga gggttacata gcgaaacttt gtacccgcac
    38041 gctgaaagac gacgcgggca atacctattc ctatgtggac gaggaaatgc gtaacaggct
    38101 gcaagtgcgc cttattaccc gcacccttgc ttttcatgta ggataatctt ttagcccatg
    38161 cggggagcgt gtcccctttc cacgcttccc gttatgggct atttgtcgtt ccgcaaaagc
    38221 atatccgctg ttgatgtgct tttgcaggcc gacaaagcct attgttcttt gacaaagaaa
    38281 gcggcctttg gtgcgcagca taacaggcaa acgggtacat tccgtctgta ccgagccagt
    38341 cggcgggtac gccatgacag cttttcccca taggggaaaa gccgagcgag aactaccgcg
    38401 ccgtaaaaca ggtttagcag cttgtgggcg acgacataca aggcggcaca atgatactcc
    38461 cgcgttgaac accctcaaag tattgcgcgg cgcacccata agcatgggcc ggggtgaaac
    38521 tcccgtgagg ttgcagctaa caaccgcccg tttttgcctt ttgacgaata tagtttatcc
    38581 atacaggaaa aggaggtttt tctaatggat aaaaaacaga caattacaac cgaacgcaaa
    38641 atagggaaaa tcacctatct tgttcaagcg ttgccgagtg aaaaggcaac cgatacaatc
    38701 cataaaaaga ttgagaaact cattgtaaag gatttgcaga aaaagcccgg aaatccggga
    38761 tttttagcgt ccgagtagtg cattaggcgg cgggatatgg tataatgata gcaacacatt
    38821 atacctgttt attcggctgt cggaaaggag gactaatgtt acagtcgaat aaaatcaccg
    38881 ccctttactg ccgtttaagt caagaggata tgcaagccgg agaaagcgga agcatacagc
    38941 accaaaaaat gatacttcaa cgctatgcgg acgaacacca ttttttgaac acaaagtttt
    39001 ttgtggacga cggattttcc ggcgtgagtt ttgagcgcga ggggctgcaa gcgatgttgc
    39061 aggaagtgga agccggacga gtggcgacgg tcattaccaa agacctttcc cgtcttggca
    39121 gaaactatct gaaaacgggc gaactcatag agattgtatt tcccgaaaac ggagtacgct
    39181 atatcgcgat caacgacgga gttgacacag cgcgggagga taacgagttt acccccttgc
    39241 ggaactggtt taacgagttt tacgcccgcg acacaagcaa gaaaatccgc gcagttaaac
    39301 aggcacaggc gcaaaaaggc gagcgcgtca acggggaata tccatacggc tatatcccag
    39361 acccgaacaa ccgccaccac cttatacccg acccggaaac cgcgccgatt gtcaaacagg
    39421 ttttcgctat gtttgttagc ggcgtgcgta tgtgcgaaat ccaaaaatgg cttgcggaaa
    39481 acaaagtctt gacgattgga gcgttgcgct atcagcgtac aggacaggcg cggtatcagc
    39541 gggcaatgat cgccccctat acttggccgg acaaaacgct ctatgacata ttagcaaggc
    39601 aggaatattt agggcatacc ataaccgcga aaactcacaa ggtatcctac aagtcgaaaa
    39661 agacccggaa gaacgaagaa gaacaacgct atttcttccc aaacacccat gagccgcttg
    39721 ttgacgagga aacctttgaa cttgcgcaaa agcggattgc tacccgccac cgcccgacaa
    39781 aagcagcgga gattgatatt ttttccggct tgctgttttg cgctggttgc agacataaga
    39841 tgtattacca acagggcgta aatatcgagc cgcgcaagtt ttcctattct tgcggcgcat
    39901 ggcgcaacag ggcaaggaca ggcagcgagt gtacctctca ttatatccgc aaaaacgtac
    39961 ttcttgattt agtgctggaa gatatgcggc gggttttgcg gtatgttaag gaacacgaac
    40021 aggactttat ctgtaaagct accgagtacg gcgacatgga agcgagaaag gcattagcgc
    40081 agcagcaaaa ggaacttttc aaagcacagg cgcgtatgac cgaacttgac acgcttttcc
    40141 gcaagctgta tgaggacaac gcattaggca gactgacaga tgaacggttt gtgtttctaa
    40201 cttccggcta tgtggacgaa aagaaatccc ttgccgcaag gatagacgag ttacaacagc
    40261 agatcgcaac cgttaccgag cgaaaaaggg atatatcaag gtttattcag attgtcggga
    40321 aatacagcga catacaggaa ttgacctatg aaaacgtcca tgagtttatc gaccgtattt
    40381 tgatacatga attagaccgg gaaaccaaca cccggaaaat cgaaatccat tatagctttg
    40441 tcggacaggt tgataccgag caggagccga cgcaagttgt caaccatgac cgccgcaaca
    40501 tggtagatgt aaaaagtatc gctatctaac cccagcaaaa aaggggcctg aaatgaaaga
    40561 gtacacgcta attatcaagg gagaaatgga ttttatcatc ttatctccgc aggtgctttc
    40621 ttccctgatt actcaaatcc ataacagccc ggagcgaaaa gttgtggtaa gtattgagag
    40681 catcatgcca ccaaagttta ccgactatct gcttcgggta atcaatagca accgctttag
    40741 caatgaacgg ttccgctatc gctacatttt agaaaatcct gttacaaaga aagggatgta
    40801 tgagatttta cggcaacagc tttcccgcac caatacggag cgatttcctt gttttcagac
    40861 catacaattg accgacactt tccagggtaa tgtggagctg gacatggagt gcaacgatct
    40921 gttcttttgg gcttgcaaag atacagcagc aaagtttgtt tacacgttcc cggatggacg
    40981 ggaggaaaca ctggtgattg aatactgaat gagaaaagcc ccatccctca atggttgaga
    41041 gatggggcaa actatttacg ctgcaatctc cgggatttcc ccgacaaagt tataaacaat
    41101 tctgacttcc tgcaccttct gaccgtccac cttcttaacc tcgccgatca ggattttgct
    41161 gataagccgg ttcagtacgg actcgtccag ttccgcaatg gcggcatagc gccggatttc
    41221 cttgataaag gttcggactt cgctttcctg tgcgtccgcc cggctctgca tcaccgccag
    41281 atcgtgcagg cgtttctgat tggcttcctg ctcctgttcc agcgccgccg tcagcttcat
    41341 aaaccgctgc tcggtgagga cgcctttggc cttatcggtg tacaggctca aaaacatccc
    41401 gtcaatttcc tgattccggt cttccagccg tttccgttcc ttctctgtct gggaagcgtc
    41461 caccagatac cggcgctcca tccggctgct gagccgctgg tagaacgcat cggcgtcctt
    41521 catggcctgc gccgccaatt cctgtatatc cttcagcacc agattgtaca aatcccttgc
    41581 ctcaatcttg tggctggtac aggcgttctt gcccagccgg ttgtaagtct ggcagatata
    41641 gtatgccctg tcaatcggct cccgcatttc gccggtgaag cggttcttcc cggttctgcc
    41701 gaccttttca taccgcacct gcatggactt cccgcaggtg gcgcagtaga caagcccgtg
    41761 gaacaggtta tagaaggggc aggcgttgcc tttcataatg gttggcctgc ggtcaatgat
    41821 gtcctgtacc tgttcccatt cctccggcgt cacaatcgct tcgtggcagc cctcgatgac
    41881 ctcccaatcc tcacggggga taatgtcata agtgttggag cggatgcctt tctggtgtgt
    41941 ccggcagaca agatgtgcgc ctttataaaa gggatttctc aggatatggc tgatccttgc
    42001 gctcccccat gaatagtagt tcacgtcaca ttctgtattg ctttttactc tggtgatggg
    42061 gattttatca tccatgagct gctttgcgat ccgcatacag ccccagccgt ccagtgccat
    42121 atcatagatt tttcggatca ccggcgcagt ctccgggtca aggatcagat gccctttgtc
    42181 ctccgggtca cgcatcaggc caagaggcgg ctgcccgccg cagaactttc cctgccggga
    42241 acgggtcatg cgtcccgcca gcaccttttt ggaaatatcc cggctgtaca tatcgttcag
    42301 gatattctta aagggcgtga tgtccatttc ctgacgggtc aggctgtcca caccgtccgt
    42361 gacggcgata tagcgtacat tgtgtttggg gaaaaagatt tcgatatagc tgccagcttc
    42421 gatataattc ctaccgagcc tcgataggtc tttcgtgatg acgcagccga ttttccccgc
    42481 ctcaatgtca gcgatcatgc gctggaacga ggggcggttg aaattgcgac ccgtatagcc
    42541 gtcatccaca taatactcat actggaacat cccatgcttt tctgcgaagt cccggagcat
    42601 gagtttctgg ttgctgatgc tcatgctctc attgtcgccg ccgtcatcca gagaaatacg
    42661 gcagtagaga gcggtgattt tttcatagac ttgttttttc ctgctcatag cgttctcctt
    42721 ctatgaacag ggacacgata ccattatagt accatgtccc cgtccctgaa tcaaccgttt
    42781 ttacgcagct ttttctgcca tttcccgctg atgacgccat tcctcgaagt gttcgcaggt
    42841 ctggcgctca atgagctgct caaaggcttc ccgcatggac ttctccccgg agaactcccg
    42901 caccaccaca aaacggactt tattcttctg atttttgctt ttttccatag gctacctcca
    42961 tagagagaca ggccgaacgg cagcaccggc aacgaagtcc ggcaacgaac ctctaaaaac
    43021 ctgtaaacta ccgtctggcc tgcaaatctt actttgtgaa ttttcttgaa aaatctcgtt
    43081 gctttcgttg ccggagataa tgataaatat taaaatatcc cttatttact ggctttttcc
    43141 gtatccagac agggaaatcc ccggcaacga acccggcaac caagtggcaa ccatcccggc
    43201 aacagaccgc cattttcagg cgatccactc cttcggaagc tccaactggc ggcattcttc
    43261 ctcgctcagt tccacaaatc catcttcgtt gccgggcagt tcgttgccgg aaacgtcccg
    43321 ttcccagccc ttctgcctgc cgtatccggc gaacatccgg ggattggaga atggcttcca
    43381 gcccgtcacc actgtattca tgatctcgtt gatattgtga agctgccacc gcttcggctc
    43441 gtcataggtg tggcccaatg cctccttaaa aagctgtttg gaacagacca tgctgccggt
    43501 ataatgttcc agaaagccct gtatttgacc ggcctccgtg tcctccggca tgaaatcctt
    43561 ctgcacctcc acaagctgcc gctggatgga cttgctgaat ttcatggaat acttcccgct
    43621 gcggtagatg gtcatggcct ccgcccagac ctgcaacagg taggctctgg aagcgtcctc
    43681 gtcctccaaa atgtgaacct ccgctttctc cgggtaaatc atgatgggaa ggaaacgccg
    43741 gttcccggcc cggtcaaggg gcaggaagtc cagcgtgttg gatgaaccgc cgaacacgca
    43801 ctgccgcagc cggtctttgg gctgggcctc atagggtgtg cggtaggttt ccttctggcg
    43861 actgatgaac gagcggattt cctctatgct tttggcgctg ctggtggcca gcatctctga
    43921 catctcaatg atccagtggc cttgcagctt caaaaatacc cggtcatcgt ccagcttttt
    43981 caggtcatcg gagaaccact cgtcccggat cgccagcagg cggaagaagc tggacttgcc
    44041 agccccctgt ccgcccacca gacagagcat ttcctcgtat ttggagccgg gagaaaacac
    44101 ccgccggatg gctcccagca ggaagtgttt taacatttcc tctacataat cgctgacttc
    44161 tgcacctaag aagtgatgga gacaggagcg gatgcggggc gtcccgtccc acacaaggcc
    44221 gttcagcacg tcctgtatgg gatggtagca gttctcattc gccacgatgg agagcgccgc
    44281 cgtcattttc ttctcgctgg tcaggccgta gttctgctca aaatagagaa gcagatactt
    44341 cacatccgta tccgtcaggg cgctggtgtt cctgcgccag cccaaatccc gcacgatgtc
    44401 cacccggtca gtcaggaggt tcagccggat ggcgtcccgc aggagagggt catgggagaa
    44461 caccgtccgg cagttgccga tggtgttggc gggctggccc ttctccgtga cagaaaggcc
    44521 ttcccgcacc tcgtcaacgc tctgctccgg cgctaaagct tcggctgcgc tcatgacggc
    44581 ctgccgggtg gctggcggta aactctgata ttcgctgctc aatcttcctc acctcttttc
    44641 catactcagc gaccacgcag gcccgttcct ccatactgcc ggacagcaga atatccagca
    44701 gatactccgt gtacggcttt ttttgcaggg cctccacaaa cagcgggttc catgcttcct
    44761 ccggcgtctg cggggcatat tccttctccc agcgttccag cagatgcaga taatcgcaca
    44821 acacccggaa acatttctgt tccgcctgcc ggtagcgcag ttcctcgctg atttttcgct
    44881 tgcccggctt cctgcggggc gggtcatggc ctttagcgtc aaagccgacc gagaaatcct
    44941 ccgccagctt caaggccgca tctttcttgt tcagcccgaa cagacgggcg gtaaaatcaa
    45001 tcacatcccc gtccgcctga cagccgaagc agtggaaccg gcggtcaacc ttcatgctgg
    45061 gatgtttgtc atcatggaag ggacagcagg tcatcccgtt cctgccgagc tgtatcccat
    45121 aagattccgc agcctgcctt gtggggacag cctgcttcac cgcttcaaac acattcgatt
    45181 ttctcacctc cagacagaga aaaaggcatc cggttttcac agaaaatcag atgccttaca
    45241 actctatatc gttctttttg gtcggggcga ttcgcccctc acgttcccaa cgctcccggt
    45301 tatcccggtc agcattaatt tttgcctttg ccagaatatc cataatggat tctttggctt
    45361 tcttcttgac ctgctccttc ccggcttgct tgacttccgg ctgcatcagc gtccgctgga
    45421 ttttcgccag agcgtgctgc atggcgtttt tgattttggt aatgacccca tccagacgct
    45481 cagcggcata ctcacgttcc ttctttggag ctttgcgctc cggcgaaagc acccattttt
    45541 tcgattcctc caccagacga atatcttcct tgtgcgtctc ctgccggacg gtatcggtca
    45601 ccacctccac cgccttgtca taggccgcat cggaaacatc atctaacagt gtctccacgt
    45661 cctcaatttt gagcgtcagt tcttccagct tctgctcctg cgccgccagt tgctccttct
    45721 gtttcatcag gatataatcc tgtttttcca gatagtcccg cccgccgtag gacggctcct
    45781 gatccagatg tagcccatgc cgtctggcga tgtcaaacag aatcgtccgg caaaccgcat
    45841 caaaggtctg cttgcggttg ttgttccggc ctttgggctt ctcagggttt gggagaggga
    45901 tgcccagttc ttccagcgcc ttttcctgct ggggacacag ttccccgtag cggttctcac
    45961 agtcaaagac gtgccgctca tggatgtggg gcgtcccctc gtccagatgg agcgcccagt
    46021 ccaggatgtg gatatgggaa ccgaagcggc gctcaaattc ctcataaaac tcattgacga
    46081 tggaaaaaag cgtgtccggc gggacggatt ctcccatcgt gccgatctgg tagatgcttt
    46141 cctccgggca ggtcttgttg tttttcagca agtcctccac ggtgcggttg cgctccgtgt
    46201 gccgggtttt ctcattccgg gcgttctggg cctccacatg gccgccgtaa tgctcgttgt
    46261 agtacatccg ctcgatttcc tcaaagctga aatccggctg ctccgggttc tcccggaact
    46321 cgtgtgtcgt aaagccccgg tagcagtccc agtagacatt ttgccttgcc cgttctgcgt
    46381 caatgtgttc gctgttctcc acatcgaacc ggcggtcatt gtggcgggga ttataggtgc
    46441 catgcttgcc ggagcgccca ttgtgtcgtg tcagtttcaa acagatttcc tccttcctgc
    46501 gaagatgttc tggggccggg gaaggggcgg cgaagccgca aatcctgcga attttgcgtc
    46561 aggtaatacc cagtacgagt tgacgcaggc atcaactctc cctgggcaag gcgtttcacc
    46621 cttgacccga taatggctgc cgcccttaac ctgccaatgg gcgttgcccc ttgaccccag
    46681 cagtgcgctg ccgcccctgc accccggcac aaagggctgg ctgccctctg tactcccgca
    46741 ccggcgaagc tggattccac cgcagaagcg gtgtttgcca gtttcaccgg ctccgtatcc
    46801 gcacatttgc gcccataagc ggggaatgga tacggaatac gcctgcgggg attgccggtc
    46861 aaaacagcgg gtgcagaccc ggcatttttt ccgtaaacgc cccggtttca ggcgattttt
    46921 tcttccgccc ctaccacctg aaagccatgc tcttttgcgt aggcgctggc ggcggctttc
    46981 cgttcctcgc tgtacggcgg caccagccgg atcgacagcc gggacttatc cagcacatag
    47041 gtcacgctgc cctccggcgt gctgcgctcc aaccggcaca gcagcgggta cttccggctg
    47101 aaatcagcca gcctgcgctt caagctggcg ttgaaggtgt agacgctggc aaggttttcc
    47161 gcctcgttcc agttgataat ggtttctttc tcatacttag acagcttcct catacagatc
    47221 ctcctcataa tcagtgtaag atttcagcac acgcaggctg cgcttgaggc ggcggtactc
    47281 gtccatctcc atccgcagat ggtggtaaaa atcctcgtac cagttttcgc ccacctccgt
    47341 ctccaccttc cgggccagcc ccagcatccg gcgtttggcc tccgggtcaa ccgtcagtgc
    47401 cgtcagccat ttcagccgtg tcaccgtatt gtggtggctg ggacagccgt aagcgtagag
    47461 aatcttcttt tctttcatac tcagtgtcat aatgatttcc tccgtttctt tgctgcggag
    47521 ccatgcgccc cgcatgattg ataaacttgt ttctgtctgc cggtctgcct gcaagccgct
    47581 cctgcccgga atttctcccg gcgtatcggc ctctttggtg cgctcgattt ctgtcctggc
    47641 gtcacttccc cggaggccat accccttgca gccggtcatt cgattttcaa ggttctgtgc
    47701 ctgtctgcaa gaaccagttt accgaaaaaa gaggcccttt cccgtatatc caaaacgtcg
    47761 gaaaaacgct tgaaatccgc atatttccac gcttatggaa tcgtgctata atgggtgtaa
    47821 tcactgaaaa acgataacga gagagggggc tttggatgta cgcaaagctg acaatcccgg
    47881 agcggctcaa agacctgcgg gtggtagata agcacctgac gctggaacaa ctggcggagc
    47941 agaccggcct gtctaaatcc gcgctgggaa aatacgaaac cgatgactac aaggacatca
    48001 gcccgtttgc gattgcaacg ctggcggact tttacggcgt gtccaccgac tatctgatgg
    48061 ggctgacgga aaataaaaat cacccaaaca tagaacttca atctctgcat ctgagtgatg
    48121 atatggtgga acttttgagc agcggcaaaa tcaacaaccg gcttctctgc gaactggcta
    48181 cccacccgaa tttccggcgg ctgatgatag atatggaaat ctgcatcgac cagatcgcca
    48241 atatgcgggt ggagcagatg aatctggtgt tggaggccac ccggcagacc gtcatgaaca
    48301 agtatgcacc cggagaagat gacctctata tgcggacgct ggaactgggg caggtgcagg
    48361 aaagcgattt ttacagccac gtcatgcacg atgatctgga cagcatcgtc cgggacatcc
    48421 gggaagcaca ccgcaaagac aagaccaccg ccgacccgca gcccacgctg gatgatgtga
    48481 aggaaaaatt tgagcaggcc atagggcagg gcagcgatac agaaatgctg atccacgagt
    48541 tctgcgataa gttgcagata ccctttgaga agatttcctc ggaggacttc tcggcgttcc
    48601 tgcggatact gagcctgtcc aagcagctca aaagctccaa caatatgagg ggaaaggcca
    48661 gccccattcc tcccaaccgc aaggccagac gtaagcggaa gtaaaatttc catatccgga
    48721 agcggcaaaa aagccatccg ccgattgatt ggcaaatggc tttcgtgaag ctgtattctg
    48781 ttgtagtggt tgtgcccagt ttgggaacag tcactattga agatgctatt ctttgtcttt
    48841 ctgcgatacc tgtgcatggg cttcctgctg aacaagcagc cccgcagaaa agccatattt
    48901 gaagtggctt tcgttttcct cacactgggc gattgccacc tgctcccgca gttcttcaac
    48961 cagtgcatag tcctctttgc tcagacgctc cgataaagtg tccatcaggg cggcgctggc
    49021 tttgactgcc tgcttgaacc tcggtgaatc agcgaccacc gtttcacagg gataaaatct
    49081 gccgttatac atttcttcca gaatcatgca cccacctccc tcagacaaaa atcatctcag
    49141 acaggatgat ttcctctacc cggctgcgga tgctgttcat gcccccgacc catgcaagct
    49201 ggtcacgggc tttcagttcc tcggtgacgc cctccgcagc ctgcatctgc gagatgatac
    49261 acgccagtcg gtcatcggcc tgctcgttca gatcggcaag atacgtccag agttttcccg
    49321 acagaagcag ttcattgtac tggccgggac ggtgttcttg cagaaacgcc ttgtgtatcc
    49381 gaccccaccg cccgatgggg cggatttcct ccgacaactt tagatcgggg atatagtagt
    49441 ctcccaccag cgtatagctg atgccgttgt gatggatttt ctttggtaaa tgctccatgt
    49501 tcgacctcct gtattcagtt ttttcagaat ccagttgatc gtgtccctgt tcatgtgaaa
    49561 ttcaaggcaa ctgaactctt cacgaacaag tatggcatat ccttaccagt cttttgatag
    49621 tttaaaccaa agctcttttg atttgacctt gtttccgacg tgttatacag gtcgatagtt
    49681 tcttttccca gtgtttcaga caattctttt agcgtagttt tctccttgcc accaagaaac
    49741 aaggtactat cacagttacc tacgattgta tcagcgttat ccttgtagat tgcctttagc
    49801 tgagattgtg cttgaagtat gatacttgct gaaatctctc ttgaacggat tgttgcaatt
    49861 aacttctcaa acttaggaat taagccgata ttagcgaact catcaagtag acacctcaca
    49921 tggacaggta atcgtccgcc atatacatca tcggctttat cacataaaag attaaataac
    49981 tgtgagtaca taatggatac tacaaagtta aatgtatcat cggtatcgga gataataaca
    50041 aatagtgctg tttttctatc tcccagtgta tcaagctcaa gttcatcgtc actcatcaag
    50101 tccctaagtt cctgaatatc aaagggagca agtcttgcac cacaagaaat aagaatcgat
    50161 ttggcagttt ttcccgcagc caatttgtac tttttatatt gcttcactgc aaagtgtgta
    50221 ggttctttct tctccagtgc ttcaaagagc ctatctatcg gattcatata ggtttcatta
    50281 tcttctctga cttctgaagc atcaatcata tcaagcagag ttgcaaagtt cttttcttct
    50341 cttggagctt catagaagat atagccaata agtgctgtat agtagagttt ctcagctttc
    50401 acccaaaaat cttcacctga tttttctccc tctcccttgg tgtttgcgat aattgtctgt
    50461 actaatttga gtatgtcctt ttcacttcta agataagcaa agggattgta tttcatagac
    50521 tttttgaagt taatggtatt taggattttt atctcatatc cgttatcttc aagcatctta
    50581 ccgcactcaa ggactatagt tcctttagga tcggtcacac aatatgacga gtgcatctgc
    50641 ataaggttcg gttttacata aaatctcgtt tttccacttc ctgatccacc gattacaagt
    50701 acattcttat ttcttgcata ctttggattt gccggtctgc cattcatggt taatcgttct
    50761 gtttgcgtaa gtaggatatt gttttgaaac ttttcatcca tatatggctc tatatctttt
    50821 ttcgttcccc atcttgctga gccatactct ttcccctgtc taaacttttt agcatttttc
    50881 cctttggtat atacgataat tttgattaaa acagctacta ccactcccat taaaatatcc
    50941 gtaggatgaa tgcttggaag aaagctcatg gtgttaagtt ccagtatacc ttgaaatatt
    51001 ttgtctatta catcgccacc agtatatgct cttacatgat gagaaaagat attgccaaca
    51061 tagaaaaatg caagataggg aatgttctgc tttagaaact ttgccttatc ttgcacctta
    51121 aataagcctt tgatgtcttt tagtatctta tctatcatag gctttgctcc ttttgtttat
    51181 ttttgatttt ctcttttgaa atagagtttt tagccatctc tttgaacttc tcaatattct
    51241 tgtgaataga ttctttcctt tctgctttct tttctgattt tgaaagagcg tgcttaaaag
    51301 ctttatccat cactttcata tctttggctt gaaagaatac agagttttta cctgtctctt
    51361 tatttttcat cactgaaaat ttaacaccgt atctattcaa ctctttttta agttctttaa
    51421 gctcagcttc ttccactggg atttcttcaa gctgtccctt tttaaccata tcttttagct
    51481 ttacttcatt tccgttggca ttgaccagtt tttcaagtcc tccgaatttc tccgcttctt
    51541 tcatcaattt ttttaataag tttaatagaa cttttccagt aactttacca gcatttatct
    51601 ccatatttaa ggtctttctt gctatttctt cattgatcac atcaatatcc tcctctcata
    51661 tcatagctta caagagccga ataataggca tctattccac ttggtgcatt ataagcggcg
    51721 gtcaggacaa aagctcttat atttctaatc ctatattcat tttctcttaa tactcctact
    51781 aagtactcta aatgtcctga attaagtttc ctaaatcttt cctttactac ttgtacagat
    51841 atctttgttt ggttgatagt aatgaaagat tcatcatcta ataccattac atctgccagt
    51901 atctttaata tttcatcaaa ctcttcagcc acactcttat tcccaatact catatgttca
    51961 taatatcctg tttggtgtcg caattcttct aaacaatttt gatatatatt tttatagctt
    52021 atatctatcc tccctccctt catattctct gaaaataatt gattcttttc tttgatggaa
    52081 ggatggatgg aaatatatgc tgaattattt gtagtattat ttgtagtatt atttgttgta
    52141 ttctctggta atggttggtg caaattatcc ttttgcatta gtgcattttg aacctgtgca
    52201 ttagtgcaat ctgcattatt gcattggctg attttgccct tatccattgg tgcattttgc
    52261 cctaatgcat tgggcataga attttttaat gcttctgttt gtattttttg ttttttcaaa
    52321 tccatatcca tatataattc ttcaagttta tcattattaa ttgtatacca ttttgtttta
    52381 tctcttggat ccttattgta atttccaact aaaagataac ctgctttttc taactttgaa
    52441 aaagttcttt ttactgtatc aaagctcata tagtcaaagt cattttcttg ccatgctcga
    52501 attgaattat atgtccagta tttaccatca tgataattct ttcctgattt tttatttatt
    52561 tctatccaat aattaatctg ctgtagtatg agtgcttcgt ttaatcctat ttctctagca
    52621 agagttttat tcgcaactat aggttgttca tcaaataaat acataactca tcaaacactc
    52681 ctttcttttt ttcaaacaaa aagacgatag ttatttctat cgtctaaaat catatataaa
    52741 tatcattttc taaatagagc gtattgcttc tttcactccc gttaaaacgg ctataacagc
    52801 tgttccaacc attggtattc cgtatgggct taacaaaaat cctaatatca atgcttctat
    52861 ccctatggta acctcttttt gtaaaaaaga tgctattgct ccaaatatgc aaaacatcat
    52921 caacaggtaa agtattgctg ttcctatgcc aagtaaaaat gaaagaaatg caatgagaat
    52981 acttaacgct atactaattg gaaataagat aatttttaat atccatctca tatatactct
    53041 ccatttatac taaatcattt taaaatgtct taaagcgtcc attatagctt cttctttttc
    53101 aggcgtacac tgtgggatag cctgattgtc ttttttagat ttattataat gctctctcag
    53161 ttcaattcca catttcttct taatctgtgc aatataaagt gtagaaacct ttagcccaaa
    53221 tttttctaat acatactcct taatttgagc atatgttgct ttgctctctg cacttgtcaa
    53281 atcaagctca tccagcttaa tttcaacatc tatatgctta tcgacatcaa gtttggacaa
    53341 taacgctacc gtctccacat gtgtagaatg agggaacata tctacaggtt gtgcctcaac
    53401 acatttaaat ccgtgaacat ttagatagtc taaatccctt gctaaagtag aaggattaca
    53461 agaaacatag acaattcttt ccggattcat tgaaacaata gtctctaata ccttttcgtc
    53521 acatcccttt ctaggtgggt ctacaactac tacattggca gtcttaccct ctctatacat
    53581 cttaggtaca atttcctctg cctttccaac ataaaattca gcattatgta tattgtttaa
    53641 tttagcattt atcttagcat cttttatggc atcatctata atttctattc cataaacctt
    53701 tttagccttt tgtgccaaaa ataaagatat tgtacctatc ccacagtaaa tatcaaaaac
    53761 agtatcagta tcatttaaat ttgcatattc aagagcctta tcatacaata ctttagtttg
    53821 gacagggttt acttggaaaa atgataatgg tgaaatctca aacactaagt cacctatata
    53881 atcgtttatt tttccatctc catatataac tatattttct cttcctagaa tagcattcgt
    53941 atctttttga tttacattta caaccaaagt cttaaatcca ggtatatttt ccttaagcac
    54001 tgaagctaat tctttaagat atggaagttt gtttccattt gctactaata caaccattac
    54061 ttctttagtt gtaaaaccta tttttgttac taaatgccta atcaaccccg tgtgagtctt
    54121 ttcatcatat atacttacct tataggccct tatatatgtt tttattattt taacaatttt
    54181 atcatttatc tcatgttgta ttacgcattt ttctgtcgat attacatcat gagtcttctt
    54241 tttatagaag ccaataattg gaataccatt attcttttgt attggaaatt gtgctttatt
    54301 tctatatcta tatggattat ccattcctat agtgtcatga acttctacat cctctaactt
    54361 acctattctc gaaagtactt gtttaacttc atttgttttc atttcaagtt gtttattata
    54421 atctaaatct tgtatctgac accctccaca atccctaaga ttgtcactac acactctttt
    54481 caccctgaat ggagactttt ctattatctc aacaatatct ccctcagcat aatttttctt
    54541 tactttatta atcctaactt taactttatc atgtattaat ccgccctcta caaaaactgt
    54601 aataccattg tactttccta ctcctactcc gccttgacca atgtctacta tatctacaat
    54661 atacatctcg tttttcgaaa gcataaatca ccctccaatc aatctattat atttaaatct
    54721 ttacaaactt ttttctttaa atttatatta aaatattgct attaattaaa ctatattttt
    54781 tactaagcta caatatttta ttttaccaaa aaaaaaggag cttataaagc cccttagtaa
    54841 ataaatttat tatttttctt ataaaactct agttgaacct ctatatacca cgcctcttga
    54901 tgcatccaca gttacaactt ctccatcttt tactagattt gttatatcag ttgcagacac
    54961 aataactggt atatctaaat ttatacctac aatagctgta tgtgatgtca ttccaccatt
    55021 ctcagttact acagctcctg ctttttctat ataattgttc atttcagcat cagtagcagt
    55081 agaaacaagt atgtcgcctt cattaaagtc tacacaagca tttccattct ttattatacg
    55141 aactttacct tctaccgttt gagtacctac acctatacct tgcacgattt cttcgcttat
    55201 tacatgaacc tttattaaat tagtagttcc acttactcct actggtactc cagcagttat
    55261 aactactaat tccccattat ctatataatc agcttgtcta gctgcctcaa ttgccttttc
    55321 tataacttca tctgtatttc ctgcaacaga agacttaact ggatatactc cccatgtaag
    55381 agctaattta ttcatagtct tttcattatt tgtagtagct attattggag attttggtct
    55441 aaatttagat accattctag cagtatatcc tgaagaagta gatgttatta tagctgatgc
    55501 atttaaatct actgctgttg tacaagttgc atggcttatt gcatctgtta cagtaacatt
    55561 gttagtacca ttttccttta gtaatctatc ataatctaaa gtctcttctg ttctttttgc
    55621 tattgtagcc atcattttta ctgcctctac aggatatttt ccagcagcag tttctccaga
    55681 taacattatc gcatctgttc catcatatat agcattggca acgtctgtaa cttctgctct
    55741 tgttggtctt ggatttctta tcatagaatc aagcatttga gtggcagtaa caactggctt
    55801 tgctaattca ttacattttt ttatcatcat tttttgaact attggcatct cttcagttgg
    55861 tatctcaaca cctaagtcac ctctagccac cattatacca tcagaaactt ttaatatttc
    55921 atctaggttc tctacacctt cttggttttc tattttagat attatttgta tatcagttgc
    55981 attattgttc tctaatactt ctcttattgc tagtacatca gatgcctttc ttacaaaaga
    56041 agcagctata taatctattc cttgacttat tccaaactct atatcactta tatctttagg
    56101 agttattgct ggtaaattta tcttaacacc aggtacattt actcctttat gatttttaac
    56161 tattcctgaa ttttcaacaa cacaaactat atcttctcca tttatttctt ttactcttaa
    56221 gccaactaaa ccatcatcta ttagtataga atctcctgat ttaacgtctt cagcaagacc
    56281 tttatagctt actgtacaca tttctttagt ccccataata tctttcatag ttatagtgaa
    56341 cttttgccct tcttctaaaa atacctctgg gtcttcaaag ttacctgttc taatttctgg
    56401 acctttagta tctaataata tagcaactgg cttatttaat ttttctctaa ctttctttgc
    56461 catatcaatt ctttctttat gttcttcatg cgaaccgtga gaaaagttaa atctacacac
    56521 atttagacca ttttgcataa gttgagttaa aatttcctca ctttgtgatg ctggacctag
    56581 tgtacaaaca atttttgtct tctttacatt atttaacata tttgctcctc ctgagatatt
    56641 attccacaga tttattttta atactaaaac ttatatagat aatatttttg ccatctcata
    56701 agcttcttta tcaaatactt tcttttgagc taaagcttca cttatttcta aatcaattat
    56761 tttgttttct tttattccta caactctagc tgattttcca tctaataata attctactgc
    56821 tcttactcca agtctacttg ctagtattct atcagataca gttgggcttc ctcctctttg
    56881 gacatgcccc aatacagtga ctcttaaatc tgctcctgac tcttttttaa gttccttttc
    56941 taaatcacta gcacttccga ctccttctgc taaaacgatt atactatgtc tcttacctct
    57001 tttttgagta gtctttaatc ttagagcaac ttcatctact gtaatttcta cttctggtac
    57061 tattatagtt tccgctcctc ctgcaagacc tgcatataaa gctaaatctc cacagtgtct
    57121 tcccataact tctactatgt taactctttc atgagatgat gatgtatctc ttatcttacc
    57181 tatagcatct ataatagtat tcattgcagt atcaaatcct atagtataat ctgtatatgc
    57241 taaatcgtta tctatagttc ctggtatgcc tatagctggg aatcctaagt cactaagttt
    57301 ttgggctcct gcaaatgaac catctccacc aattacaact aagcaatcaa ttttatactt
    57361 tttaagaatt tttactgctt ttaatctacc ttcttctgtt ttaaattctt cacatctgga
    57421 agattttaat atagttcctc ccctgtgtat aatatctcca acagaagata agttcatttc
    57481 agttaaatct tcttctagta atcccttata tcctctattg attccatata ctttgcatcc
    57541 ataatatatt gctgatctta caaccgctct aatcgcagca ttcattcctg gagcatctcc
    57601 tccacttgtt agtaatccta tagttttcat tttttacctc cttggatttt atgtcaattt
    57661 tttattataa tcattgttat tgtattctaa tggacttttt ttgtccctat atcacttttt
    57721 aatcccatca agactaaaaa aattaaatgt ccttaaatgt ccattttgtg atattatatc
    57781 acaaaacgga caatattttt catattatta taatatattt agatttttat ataaaaaatc
    57841 aagcttttac ttttatgtta tctttaggaa gtaactcttc taagttattt ataaattcat
    57901 cacatataca agtcaatgcc ccggtcattt tatatgcttt acgtttacca tttatatttt
    57961 catccatagg gtacaagtat atactgtcat tacctggata ttgtttggct atttctacaa
    58021 tattattagt tgtatcagta tcagacatac tatctatttt caaatacaat tttttaccac
    58081 tttctggctt aatattttct acatttctag ttcttcctga attgtagttt ctatctgcaa
    58141 agtcagtatc atcatttata tcttttatct ctctagctat aagttttgca ttttcgcctt
    58201 cttttatact taatgttcct tttacatata ttatattatc ttcattcaaa atgacattat
    58261 acttttgtaa taattgagga aaaactatta tttctatagc accatacaaa tcttctaatt
    58321 caataaatgc cataatttca tttcgttttg ttgttttaat agttttagtt acaatcattc
    58381 cacccattat aacatctgtt tcattcattt gtaggtatga ctcttcttcc tcttttaatg
    58441 aatttaattt tccattatcc atagatgttt tttcttgtag ttctttttca aattgtgata
    58501 aaggatgtcc acttacatac attcccaaga cttctttttc aaggttaagt ctctctcttt
    58561 cttcatactc ttgcctttta ggtaaactgt atatttgttg aaaatcacta tcttcctcac
    58621 tatctccaaa agcatcaaat aaagaaattt gaccttgaac atttttcttt ctatccatag
    58681 atacactttc taataaactt tcataaccag ccatcagact agctctattt tcacttatat
    58741 tatcaaaagc tccacatttt attagacttt ctattactct cttatttgta tctttttggt
    58801 ctaatctttt gactaaatct actaaatcta caaataatcc attagattct ctttcagcga
    58861 ttatattgtt tattatattt acaccaacat tttttacagc tgctagacca aatcttatat
    58921 tgtcgccttc aactgaaaac tttgaaaaac ttttgtttat atctggaggt aatacatcta
    58981 tttctagtgc tttacattct cttatatact caactacctt atcagtattt cccattacac
    59041 tggttatcaa tgctgccata aattcaactg gataatatgt ttttagataa gctgtctcat
    59101 atgctaaaac cccatatgca gctgcatgtg atttattaaa tgcatatcta gcgaagtcta
    59161 tcatatcatc aaatatttta ttagctattt cctcttctac accatttctg atacaccctg
    59221 caatttctat attaccatct tcatcaaatt taccatgaat aaaatattgt ctttcttctt
    59281 ccatcacgtc catctttttc ttactcatgg ctcttctaac taggtcactt cgtccgtaac
    59341 tataaccacc taaatctcta acgacctgca taacctgctc ttgatatacc aaacaaccat
    59401 atgtaacttc cattataggc tttaattttt catgtagata tgttacatct tttgggttgc
    59461 ttttattttt tatatatgca ggtatagaat ccattggacc tggtctaaat agggatattc
    59521 ccgctacaat atcttcaaaa ttgtctggtt ttagctgttt cataaaactt ctcatgccag
    59581 cactctccaa ttgaaacacc cctagtgtat ttcctgatga aagcatttca tatactttcg
    59641 ggtcgtcata atccatcttt gaaaagtcta ttttttctgt gtagtttttt ataactctat
    59701 ttttttctat taaatcaaga gtatctctta taactgtaag agttcttagc ccaagaaaat
    59761 ccatctttaa aagcccaagt tcttcaagtg ttgtcatagt aaactgtgtt gtgattgcat
    59821 cttgatgttt gtacaaaggt acatattcat ctacgggatt ttttgatata acaactcctg
    59881 ctgcatgagt agaagcatgt cttagcattc cctctatctg ttttgatata tttattattt
    59941 cttttgtttc cgggtcttgg tcatacaatt ctttaagatt aggatttgta tctagggcct
    60001 tatctattgt cattcctaaa gcaaatggta tttccttggc taccttgtca actttattgt
    60061 agcctatatc caataccctt ccaacatctc ttattgcagc ttttgcaccc attgttccaa
    60121 aagttattat ctgggctaca tggtcatcac catattttct ctttacataa tctataactt
    60181 cttctcttct ctcataacaa aaatctatat ctatatctgg catggatata cgttctggat
    60241 ttaaaaaacg ctcaaataat agagagtact ttattgggtc tatatcagtt atcttaagtg
    60301 tatatgctac tatcgaacca gccgcactac ctctacctgg tcctactata atattatttt
    60361 ctttagaaaa atttataaaa tcccaaacaa ttaaaaaata ctctacataa cccatttttt
    60421 caattacatc taattcataa tttaatctct ctagtatctc attgctagga ttatcatatc
    60481 tctcttttaa tccattaaaa cacaactccc ttaaataagt ttctggtgta tatccttctg
    60541 gaacatcata cttaggaaga tgtatagtat taaaatcaaa ctccacatta cacatatcag
    60601 ctattttcac agtattgtca atagcctcta atgcgtatgg aaataattct tccatttctt
    60661 ctcttgattt taagtaaaac tcatcactac caaatctcat tctatttggg tcatttaatg
    60721 tttttcccat ttgaatacac ataagaacat catgtatttt cgaatcttct ttatttacat
    60781 agtgaacatc attagtagca actagtggta taccagtctc ttttgataat tttactaaac
    60841 cagagtttac ttctttttgc tcaggtaaat tgtgatcttg aatctccaaa aagaaatttt
    60901 cttctccaaa tatgtctctg tagtcaagag caaattgttt tgctttttca taatttctat
    60961 tcattaaagc acgtgctaca tctcctgcaa ggcatgcaga aagtgctata atccctttgt
    61021 tatgtttttt aagttcttcc atgtcaactc taggcttata gtaaaaacct tctacgtatg
    61081 aagttgaaac tatttttatt aaatttttat atccttccat attcttagct aacagaacta
    61141 aatgtccttg atatttgtca tagtttgggt ctttatctct caatccacgt gcagctgtat
    61201 agacttcgca ccctataatt ggctttattc cttcttttat agctgttttg tagaaatcta
    61261 taacaccaaa catacaacca tggtcagtta tagctattga gctcatgttt agttccttag
    61321 ccctttttat aagttttttc accctcgaaa acccatcaag taaactatac tcagtatgta
    61381 catgaagatg agcaaaatct ttgttcatta aatcacctcc tgctttataa ttttaatttt
    61441 accacaaata tatgtcctaa aacaaaaaca gagactttta tctcaaaata ggaattaact
    61501 tcaacatata ccattatatg ttataaatta atgcttattc tgagattgtc ccctcaattt
    61561 attgctatga tttttttttc aaatctgttg caatttcttc tatttttcta agtctatgat
    61621 tgactccaga ttttcctatt ggtggttcta acatctctcc aagttctttg agagtcatat
    61681 cctcatactc gattctaatc ttagctatct cttgtaaatt ttctggcaaa ctgctaattc
    61741 ctatagtctc ttgtatgagc ttaatatttt ctacttgtct tactgctgca tttactgttt
    61801 tagaaaggtt agctgtctca caatttacta tcctgtttac attatttctc atttctttta
    61861 ctattttagt attttgaaga cttaaaagag cgtggtgtcc tcctataata ctcaacaaat
    61921 ctgatatttg ttcactctct ttgatataaa caacgtgatt attttttcta gccacaattt
    61981 tactattaaa acctaaagag tttataagtt cttttagaga atctgcaaac tcctcattat
    62041 tagttacaaa ttccatatga tagttctttt ctgggtcgct tatagaacct ccaccaagaa
    62101 aagctcctct tatgaaagct ctcttacatc cttcatgctt aattaagttc tctgaaattt
    62161 tgtttatagt aaaaaagcct tctttatctt caagtatacc taactttttt aataacattt
    62221 ctgaacccat ttcgcttgtt accattaata cataactgtt atttctcttt agcatttgat
    62281 tcttatttac tgagattgta gtattgatat taaaattctt ttttaataat ttaaatactt
    62341 tacgggctat tgaatttaac tctgttgtta tctttagatt tagcttctta tatccaacga
    62401 tttgtatact tccccctgat ttaactagag ctgataattc agctatatta caacattcat
    62461 tcccagatat aactcttgct agttcatttt tggtttcagt tgaaaaagac atatctatca
    62521 cctattaact aatttttctt taaccttttt tttatttctt gtaaggcaac ctttattatc
    62581 tccttttcat catcatacct agctaggtct accactctat ctagatggat aaatttagca
    62641 tcaataaagc cttcctcttt ttgtggaata gtatccatat tctttgcttg catcaaatac
    62701 caaaaaactg ttttatgaac tgctctattt tcatcccagt tttctttgaa tgtatagtgt
    62761 atttcaccta aatatttcaa tatatctgct tttactccag tttcttcact tacttcacgt
    62821 aatgctgcct cttggttatt ttccccttct tctacttttc ccttgggtaa cacccaatct
    62881 ccattgaact tccttagtaa aagaatcgta ttgccaaaca ggactacacc accagcacta
    62941 acctcttctc tcatcgatgt tcactcctta aaactaacat aatgataagg atttctacac
    63001 aattttttaa aatccttttt tactaacttt tgttgtaatt atgatttagg gctaactcaa
    63061 taactatgtt agatatatac tttgcatcat gtcttatata attatttttt atttcaatta
    63121 aattcttctc tacggttttt atacccatct cttttaattt atctttttgc tttttatcta
    63181 acaagacttg ctctgctcca tcttgtttat ataagtcaag catttcttct ggcaatatct
    63241 cattatttgc gattacataa tctattaaat tctcatcagt gtgctttact atagcattaa
    63301 catgctctaa tacattgtac ccttctgtct cacctggttg agtcattata tttgctatat
    63361 atacttttgg agctacagac gatttaatag cgtctacaat cccctctaca agtaaattgg
    63421 gtattatact cgtatataag cttccagggc ccataattat aatgtcagca tcgtaaatag
    63481 atgcaataac atcctttaat ggtttgacat cttttggatt taaaaaaatt ttatcaatag
    63541 agcttttttg aatttttgac tcttcaggta tactagattc tccatttatt atattgccat
    63601 tttctaattt ggcaactaaa tttacatctt ccaaagttac tggtaaaact cttcctgtta
    63661 tagcaaaaat ttcacttaat ttatatacag ctttttcaaa gtttccatac aacccattca
    63721 tagcagccaa aaacaaattt ccaaaactct gaccttttaa aagcccttct gtaaacctat
    63781 attgcataac ttcattcatt gttggctcta tatttgcaag tgctaataga cagtttctta
    63841 tgtctcctgg tgggagcatt cctaagtctt ctctcaatac acctgaacct ccaccatcat
    63901 cagcaacagt tacaattgca gttatatttt gcgtagtgtg tttaagacct cttaaaaata
    63961 ctgattgacc agtacctcca cctatcacaa ccacatttgg ttctctattt tcaacaatag
    64021 acctttgaaa tagttttact ttttcatttt ttgctttttt atatataaat aacgatacaa
    64081 ttaatgcaat ccaacctaaa attcctacaa taataagtaa tttagccatg tttgcctcca
    64141 ttatcgcaac atagagtctc tatgtttctt tacaactcta tatcccttat tagataaatc
    64201 ctccgctata agatttgtta tagtaactga cctatgtctt ccacctgtgc atccaacacc
    64261 aattactaga tgttgttttc cttcatctat atattgaggt actaaaaaat gaatcatatc
    64321 taaaagtttc acataaaatt cttcactgat tttagaattc ataacataat ctctaacttc
    64381 tttatcatca ccagttttag ctcttaactc ttcaacataa tagggatttg gaagaaatct
    64441 tacatcaaat actaaatctg catcagcaag tataccatgt ttaaatccaa aagatactac
    64501 agaaatagtt aggtttggat tatcttcacc agaagaatat attttcttta tttcttcctt
    64561 aaggtcttta ggtttcatgt tagttgtatc aattatacat gtagataact cctttaaagg
    64621 ttccattatc tttctttcca tttttatacc ttctggaatc tgcatatctt ttgccaatgg
    64681 atgatttctt cttgtcattt tatatctctt taacaataca tcatcattac agtccaaata
    64741 taccatttca tattcataat tttctttttc taaataattt aagctttcat gaagcgcttc
    64801 aaaaaacttt ctccctctta tatctatccc tagagcaact ttatcaatac ttgaatttgg
    64861 ttgataacat aattctgcaa atttagttat caaagcagga ggtagattat ctacacaata
    64921 aaatcccata tcctctaaag ctctcattgt ctcacttttt ccagatcctg aaagacctgt
    64981 aactataaca aatttcattg ctaactcctc ccttttatct tatgtttatt atattataat
    65041 gttttctact aacgatatat ccagattaga gtcagatata gtcttcatag ctagtttaaa
    65101 atcaccaact ctttctggac aatgtgcatc tgagcctatt ataaatttat tttctagccc
    65161 ttttatttta tttaattgat ttacattcaa aaaagaatgg ctactattta tttcaagtct
    65221 agtatttgtc ttttttgctg tctttgctac ttcttctata tacacatcgc ctttatctcc
    65281 tggatgagta attataaaaa tatcattatt acttatcgca tttattacag cttttgtatt
    65341 gtattctttt gctttttcag ttttaaaaat atagttatta caatgattta ataagcttct
    65401 taatgatgtt ggagtagaac caaaatgata tccagccata atataatcaa aatctctaat
    65461 aaatttatca tccatatcta tattgccttt atcatctaat atgttacact ccatacctaa
    65521 aagtatattt atatcattgt acttatcatt taaataatct acttcttctc tcattttgag
    65581 aatatctttt ttctttacac catatgcaat atgtttatag ctatggtcag atattcctat
    65641 tgtttttatt cctttagaaa tggctacctt tacattatct tcaatagtcc ctttgccatg
    65701 actatataat gtatgtgtat gataatcagc cagtattttc atttgttaat cctctccaag
    65761 tattttgact tcttcttcca acataacccc aaacttggca tatacagcac tttttataac
    65821 atatattaaa tccaaaatat ctttagcact tgcttctcct tgattgacta caaatccaca
    65881 atgcttctca gatacttgtg ctcctcttaa agtaagacct ctcaatccag tttcttctat
    65941 taatttacct gcaaaatatc ctgttggtct cttaaatgta cttcctgcac tcgcaaaatt
    66001 taatggctgt ttagttattc ttcttgtcgc taaatcatcc attaaacttt ttatttcatc
    66061 ataatttcct tcttgtaatt ctagttctgc tgataaagct atatatccgt ttttcgatat
    66121 tatacttttt ctatacccaa attccatctg ttcatttgat aattcaaata tattgccttc
    66181 catatccatc agtcttactg acttcactat gtccttcatt tcaccaccat atgctccagc
    66241 attcatggct agtgctcctc ccaatgtacc aggtattcct gatgcaaact caaacccttt
    66301 taattcttgc ttttggagag ctttacctaa aactgataat aaagctcctg actgagcagt
    66361 catcctagta tcatttatct cataagaatt gaaattatca gctaattcta ttaccactcc
    66421 tcttattcca ccatctttaa ttaaaatgtt tgaaccattt ccaattatta aatacggaat
    66481 agattcttct tttaccaact taagtacatt ttttaattgt tcttcagttc taggtctaac
    66541 taatatatct gctggtcctc ctaccctaaa tgaaatatgt tttttcatag gttcatcgac
    66601 ttttatatcc tctttactta atatatttaa taaatttata tatatatctt gattattcat
    66661 tgtatttcct cccaaattat actgaacttt agctgtcatt attatttaaa ttacattagt
    66721 atatagtatt aactatattc aataatatat gattttgctt atcatatgga ttttatattt
    66781 taagcttctt aattacaaac tatattcaat aaatatataa taaagtaaaa aaaaaaaaat
    66841 aacaacatat aaaagaatat aaataattta aaaaaagcat caatatcact gatatcaatg
    66901 ctttaaaagg caatttaatt cgtattattt atctaactat ttttgacata gcaaagataa
    66961 taaatccctc caactactat ggaacctcca acgatatttc caagcgttac aaataccaaa
    67021 ttagttataa attgaaccat agttatattt gctcctaatg ccattcccat tggtataaaa
    67081 aacatgtttg caacactatg ttggaatccg cataatacaa ataacattat tactgtccaa
    67141 catgaagcca attttgacat tatatcttga gatgctgtag ccatccatac agctaaaacg
    67201 actaaaatat tacaaagtat tgcttttaaa aacattgatg ctatagttga attaacctta
    67261 gcttctgcca cagctatagc ttttgtagct gcatctcctg tcaatgtaga agaataaaac
    67321 acaagtgcaa ctagcagaat tgaacctact aaatttgcaa aataaaccag tccccagttt
    67381 cttaacattt ttcctaatgt tatttttttg tccataagtg caatagccat taaggtgttt
    67441 ccagtgaata attctgctcc tgccaccact acaagcataa gacctactgg aaagaccata
    67501 gcacttaaaa agctagctac ccctgggtct atcttgccaa aagtttgact aactatcata
    67561 ctccccaatg ccccaaaacc tataaatgct cctccaaata aacctaatag aattgcattt
    67621 tgtgtcgaaa tgtttgcttt tttaatgcct gcacttatag ttgcttgtgc agtttcccca
    67681 ggtgtcaaga atcctttact cataaatcct tcccccttaa aaattatact ttcccttgtc
    67741 tttttatggc aataattata taataacaat atgatatata ataaatttta ttaacataaa
    67801 aaatatacta tatacttata tatacttgtc tcatataatt atactattta ttatataaca
    67861 actttgttaa tttcgaaata atttatattg aatttgaaaa atttaccttt tgattattta
    67921 ttttac
//
LOCUS       DAEPOG010000003        62275 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEPOG010000003 DAEPOG010000000
VERSION     DAEPOG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.003"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            736..1758
                     /locus_tag="KR432_000129"
     CDS             736..1758
                     /locus_tag="KR432_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0638691.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            2054..2974
                     /gene="pyrB"
                     /locus_tag="KR432_000130"
     CDS             2054..2974
                     /gene="pyrB"
                     /locus_tag="KR432_000130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH0638692.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            3013..3705
                     /locus_tag="KR432_000131"
     CDS             3013..3705
                     /locus_tag="KR432_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBH0638693.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3698..4600
                     /locus_tag="KR432_000132"
     CDS             3698..4600
                     /locus_tag="KR432_000132"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0638694.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4685..5269
                     /locus_tag="KR432_000133"
     CDS             4685..5269
                     /locus_tag="KR432_000133"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH0638695.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5484..6410
                     /locus_tag="KR432_000134"
     CDS             5484..6410
                     /locus_tag="KR432_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBH0638696.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(6462..7352)
                     /locus_tag="KR432_000135"
     CDS             complement(6462..7352)
                     /locus_tag="KR432_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0638697.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7540..8571
                     /locus_tag="KR432_000136"
     CDS             7540..8571
                     /locus_tag="KR432_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBH0638698.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8865..9740
                     /locus_tag="KR432_000137"
     CDS             8865..9740
                     /locus_tag="KR432_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBH0638699.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9758..11248
                     /gene="cls"
                     /locus_tag="KR432_000138"
     CDS             9758..11248
                     /gene="cls"
                     /locus_tag="KR432_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0638700.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11441..11725
                     /locus_tag="KR432_000139"
     CDS             11441..11725
                     /locus_tag="KR432_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH0638701.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11758..13386
                     /gene="groL"
                     /locus_tag="KR432_000140"
     CDS             11758..13386
                     /gene="groL"
                     /locus_tag="KR432_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH0638702.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13648..14388
                     /gene="larB"
                     /locus_tag="KR432_000141"
     CDS             13648..14388
                     /gene="larB"
                     /locus_tag="KR432_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBH0638703.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14472..15206
                     /gene="larC"
                     /locus_tag="KR432_000142"
     CDS             14472..15206
                     /gene="larC"
                     /locus_tag="KR432_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBH0638704.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15236..15655
                     /locus_tag="KR432_000143"
     CDS             15236..15655
                     /locus_tag="KR432_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBH0638705.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15715..15816
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            16037..17572
                     /gene="guaA"
                     /locus_tag="KR432_000144"
     CDS             16037..17572
                     /gene="guaA"
                     /locus_tag="KR432_000144"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH0638706.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            18111..19541
                     /locus_tag="KR432_000145"
     CDS             18111..19541
                     /locus_tag="KR432_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0638707.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19689..20564)
                     /locus_tag="KR432_000146"
     CDS             complement(19689..20564)
                     /locus_tag="KR432_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0638708.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            20684..21247
                     /locus_tag="KR432_000147"
     CDS             20684..21247
                     /locus_tag="KR432_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0638709.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            21244..21810
                     /locus_tag="KR432_000148"
     CDS             21244..21810
                     /locus_tag="KR432_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0638710.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(21939..22175)
                     /locus_tag="KR432_000149"
     CDS             complement(21939..22175)
                     /locus_tag="KR432_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638711.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(22485..25913)
                     /gene="uvrA"
                     /locus_tag="KR432_000150"
     CDS             complement(22485..25913)
                     /gene="uvrA"
                     /locus_tag="KR432_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0638712.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            26232..28466
                     /locus_tag="KR432_000151"
     CDS             26232..28466
                     /locus_tag="KR432_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0638713.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28722..30752
                     /locus_tag="KR432_000152"
     CDS             28722..30752
                     /locus_tag="KR432_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0638714.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30749..31201
                     /locus_tag="KR432_000153"
     CDS             30749..31201
                     /locus_tag="KR432_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0638715.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            31203..32258
                     /locus_tag="KR432_000154"
     CDS             31203..32258
                     /locus_tag="KR432_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH0638716.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            32325..32633
                     /locus_tag="KR432_000155"
     CDS             32325..32633
                     /locus_tag="KR432_000155"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH0638717.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32737..34002
                     /locus_tag="KR432_000156"
     CDS             32737..34002
                     /locus_tag="KR432_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBH0638718.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            34090..34806
                     /locus_tag="KR432_000157"
     CDS             34090..34806
                     /locus_tag="KR432_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0638719.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(35001..35294)
                     /locus_tag="KR432_000158"
     CDS             complement(35001..35294)
                     /locus_tag="KR432_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBH0638720.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(35300..35515)
                     /locus_tag="KR432_000159"
     CDS             complement(35300..35515)
                     /locus_tag="KR432_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638721.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(36060..36572)
                     /locus_tag="KR432_000160"
     CDS             complement(36060..36572)
                     /locus_tag="KR432_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0638722.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            37022..37498
                     /gene="purE"
                     /locus_tag="KR432_000161"
     CDS             37022..37498
                     /gene="purE"
                     /locus_tag="KR432_000161"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH0638723.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            37504..38202
                     /locus_tag="KR432_000162"
     CDS             37504..38202
                     /locus_tag="KR432_000162"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0638724.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            38214..39581
                     /gene="purF"
                     /locus_tag="KR432_000163"
     CDS             38214..39581
                     /gene="purF"
                     /locus_tag="KR432_000163"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH0638725.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            39575..40651
                     /locus_tag="KR432_000164"
     CDS             39575..40651
                     /locus_tag="KR432_000164"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH0638726.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            40645..41238
                     /locus_tag="KR432_000165"
     CDS             40645..41238
                     /locus_tag="KR432_000165"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH0638727.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            41231..42763
                     /gene="purH"
                     /locus_tag="KR432_000166"
     CDS             41231..42763
                     /gene="purH"
                     /locus_tag="KR432_000166"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH0638728.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            42774..44024
                     /gene="purD"
                     /locus_tag="KR432_000167"
     CDS             42774..44024
                     /gene="purD"
                     /locus_tag="KR432_000167"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBH0638729.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            44049..47855
                     /locus_tag="KR432_000168"
     CDS             44049..47855
                     /locus_tag="KR432_000168"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBH0638730.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            48061..48933
                     /gene="rfbD"
                     /locus_tag="KR432_000169"
     CDS             48061..48933
                     /gene="rfbD"
                     /locus_tag="KR432_000169"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBH0638731.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            49042..49923
                     /gene="rfbA"
                     /locus_tag="KR432_000170"
     CDS             49042..49923
                     /gene="rfbA"
                     /locus_tag="KR432_000170"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBH0638732.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            49960..50517
                     /gene="rfbC"
                     /locus_tag="KR432_000171"
     CDS             49960..50517
                     /gene="rfbC"
                     /locus_tag="KR432_000171"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBH0638733.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            50555..51538
                     /gene="rfbB"
                     /locus_tag="KR432_000172"
     CDS             50555..51538
                     /gene="rfbB"
                     /locus_tag="KR432_000172"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBH0638734.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            51577..52245
                     /locus_tag="KR432_000173"
     CDS             51577..52245
                     /locus_tag="KR432_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0638735.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            52254..52682
                     /locus_tag="KR432_000174"
     CDS             52254..52682
                     /locus_tag="KR432_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBH0638736.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            52706..53020
                     /locus_tag="KR432_000175"
     CDS             52706..53020
                     /locus_tag="KR432_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0638737.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            53172..53447
                     /gene="flgM"
                     /locus_tag="KR432_000176"
     CDS             53172..53447
                     /gene="flgM"
                     /locus_tag="KR432_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBH0638738.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            53452..53868
                     /locus_tag="KR432_000177"
     CDS             53452..53868
                     /locus_tag="KR432_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBH0638739.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            53886..55196
                     /gene="flgK"
                     /locus_tag="KR432_000178"
     CDS             53886..55196
                     /gene="flgK"
                     /locus_tag="KR432_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH0638740.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            55215..56147
                     /gene="flgL"
                     /locus_tag="KR432_000179"
     CDS             55215..56147
                     /gene="flgL"
                     /locus_tag="KR432_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH0638741.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            56163..56555
                     /locus_tag="KR432_000180"
     CDS             56163..56555
                     /locus_tag="KR432_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBH0638742.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            56549..56761
                     /gene="csrA"
                     /locus_tag="KR432_000181"
     CDS             56549..56761
                     /gene="csrA"
                     /locus_tag="KR432_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH0638743.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            56783..57178
                     /gene="fliS"
                     /locus_tag="KR432_000182"
     CDS             56783..57178
                     /gene="fliS"
                     /locus_tag="KR432_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0638744.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            57178..57534
                     /gene="fliS"
                     /locus_tag="KR432_000183"
     CDS             57178..57534
                     /gene="fliS"
                     /locus_tag="KR432_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0638745.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            57555..59078
                     /gene="fliD"
                     /locus_tag="KR432_000184"
     CDS             57555..59078
                     /gene="fliD"
                     /locus_tag="KR432_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH0638746.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            59081..59416
                     /gene="fliT"
                     /locus_tag="KR432_000185"
     CDS             59081..59416
                     /gene="fliT"
                     /locus_tag="KR432_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBH0638747.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            59518..60390
                     /locus_tag="KR432_000186"
     CDS             59518..60390
                     /locus_tag="KR432_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBH0638748.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            60483..>62275
                     /locus_tag="KR432_000187"
     CDS             60483..>62275
                     /locus_tag="KR432_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0638749.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSM"
BASE COUNT        24481 a         6737 c        11203 g        19854 t
ORIGIN      
        1 aaaattagat aaattcaggt gtaaaatact ataaaacaca attcggaaaa gtgtatgttg
       61 aacttcattt aaaatagaat tttcaaagtt gttatagtaa gtagtttaag cattcttctt
      121 atattaatct ttgagaataa tgtacttaaa aaagttagta aaaaatttaa ctattgtata
      181 ttaatgatag ttgctattga tatattatct aaaaaaatta agtatgaaaa gattactgct
      241 aggacaatct tttcatactt aattttttaa tatttatatt ctccaaaatt ttcatctttt
      301 taacttagaa ttattagaat ttgagaatca ctattaggaa ataggttttg atttatgcag
      361 ggttaaaaat ttcacagaat atctaacttt atactaaatc catcaaaaaa tatacgccat
      421 ataaaagcgc ataaaaaaac acataaaaca atatttatgc gcactttaac ctcgaatggt
      481 acaaaattct ttacttttac agacccatat tatataatta agtaaataat gtatatttag
      541 gtgacatatt ggataagcta atttatttat tttattaata taatgatact ataaaagtta
      601 tataaagtgg tttacaaaaa agttacagat agagctttta ttaatgtatt gtgtattagt
      661 attttatcct taggtacact tcacctacaa aatagaatca taagataatt atgggaaaat
      721 tttaggaggt ttatcgtgaa gaaaaagata ttaataccag tatttgcatc agttatggca
      781 ttatcagtaa gttcaattgt aaatgcagat gaagtaaatg attcatctca aaataaggat
      841 gataaaacaa atactgaatt aaatttaggt gaatataaag aagtaaaata taaagttgca
      901 aaaataaaag atggagtagc tataaagata agagaagaag gtcaagttca aaacatagct
      961 tattctggag atgaatttac ggttttagga actcaaggag aatgggttaa ggttaaagtt
     1021 gaagatggcg aaggttggtt agctactaga tatgtagata tatctgaagg tgtaggatat
     1081 actaacgctg ataaagtaaa cttaagaaaa gataagagtg aaagttcaga agtaattgaa
     1141 gagctggaaa aaggaagttc tttattggtt ttggaggata atggagattg gttaaaagtt
     1201 aaagatgggg aaacagaagg atatgtaaaa tcatcttata tatctgataa agctcctgta
     1261 atagaagagc cacaagtaaa cccaagtatt gaccaaaatg tagattctac aaataataat
     1321 caacaaaaca atgcaaatac taatcaacaa aataataatt caaatgtgcc aacagctaac
     1381 tctaatgcag ttcaagctgt acttaacttg gcatattcta agcaaggttg tccatatgtt
     1441 tggggagcag aaggaccaaa cacttttgac tgttctggat ttacacaata tgtgtataga
     1501 aatgcagtag gaaagaatat accaagaaca tctaaagcac aatctaaata tggacaaact
     1561 gtaagtaaag caaatttaca accaggagac ttagtattct tcacaactaa tggttctggt
     1621 tctgttagtc atgttggaat atatgtaggt gggggtaata tgatacattc accatcaaca
     1681 ggaaaaacag ttagtgtaac tagtataaat tcatcatact atacagcaag atttgttaca
     1741 gcaaaaagaa tattataatc ggtttgtaat atgacaaatt gataaataca taaatatata
     1801 taaacgctct atattatatt taatttaatc atggaaacaa aaaatgatta tattttaaat
     1861 gtactataga gcgtttttgt atcactttaa atctaatcaa gattcaagtt aatatgacta
     1921 ttaaatagga gccttttcat atgatgatta tttgcgcaat cccttgacag gatagggtaa
     1981 aggtggtaag ctactaacag tacaaaaata cttttaattt tagttcagag aggctaaaaa
     2041 aggaggacga aagatgttaa aatcaagaaa tttaatccaa ccagaagact tttcaataga
     2101 agaaatagat gaaatattag aattagctca aaaaataatt gacaatccgt caaaatattc
     2161 tcgtatatgt gaaggaaaac tattagctac attattttat gaaccatcaa caagaacaag
     2221 attaagcttt gaatctgcta tgaacagatt aggaggacgt gtagtaggct tttcagagcc
     2281 aaattcatcc tctgcatcaa aaggtgaaac tctaggagat acaatgagga tagtatctgg
     2341 ttatgttgat ataatagcta tgagacatcc tcaatcaggt gcagcttcag aagcagctag
     2401 atatacggaa gtgccattta taaatgcagg agacggcaaa aaccagcatc caactcaaac
     2461 acttacagac ttacttacta taaaatcatt aaaaggaact ttagaaagcc atacaatagg
     2521 attatgtggt gatttaaaat atggaagaac tgtgcattct ttggttaagg ctatggcaag
     2581 atataaaaat acaaagtttg tatttatagc gcctgaagag ttaaaaatgc cagattatat
     2641 aaaagaagct ataaagggac atgcatatta tgaaactaat aatttggatg atgttatagg
     2701 aagcttggat gttttatata tgactagagt tcaacaagaa aggtttgaag ataaatcaga
     2761 gtatgaaaga cttaaaaact attatatttt aaataaagct aaattagaaa aggcatctaa
     2821 agatatgtta gtaatgcatc cattaccaag agtaaatgaa atagacatag atgtagatag
     2881 tgatgataga gctgtatatt ttaaacaagc taaatatgga atgtatgtta gaatggcttt
     2941 aataataaaa cttttaggta taaatgaaga ctaatagtta tcatataata gtaaaacaaa
     3001 ggagattttg ggatgtataa aattctggaa aatatatata taggcgaaga tatgtataga
     3061 atgaaagtaa aaggtaattt tgaaggtaaa atgggacaat tctatatgtt aagagcatgg
     3121 gatacttatc cagtactttc aagacctata agtatacatg atatagatga agaagggata
     3181 acttttcttt ataaagtagt aggagaagga actcaaattt tgagtaacct aaaggtaaat
     3241 gataatataa aattagaagg cccttatggt aatggatatg ctaaggttga tggaaaggta
     3301 gctctagtag gaggaggaat cggtgtagct ccactatatt tggttgctaa aaatataaaa
     3361 aattgtgatg catatcttgg gtttagagaa gatgttatat tagaagatga atataagcaa
     3421 gtatgtaata aagtctatac aacagtagga aatacttttg taacagatat aattgatgtt
     3481 gagaaatatg attatatatt aacatgtggt ccaactccaa tgatggaaaa attagttaag
     3541 atggtagaag gtactaaaac gagaattatg gtatctcttg aaaaccatat ggcttgtgga
     3601 gtaggtgcct gtttagtatg tacgtgtaaa actaatggag gaaacaaaaa gacttgtaaa
     3661 gatggtccag tgttctgggg agaggacgtg atttttaatg ggtaatttaa gtgtgaaatt
     3721 tggaagtgta gaatttaaaa atccagtaat aatggcgtct ggaacttttg gatttggaaa
     3781 agaatataat gagatttatg atatacaaaa acttggtggt ataagtagca agggtcttac
     3841 tttaaataaa aaacctggta acaatggtat gagagttcat gagacaccat ccggtatgat
     3901 gaatagtgtt gggcttgaaa accctggagt tcaaggattt atagactatg aattaccttt
     3961 ttttagtaaa ctagacttag tcagaatagc taatgttgga ggtgggacat tagaagacta
     4021 cttattaggg gttcaaatgt taaatgacaa gcctatagac attattgaat taaatatatc
     4081 ttgcccaaat gtcaaagctg gtggaatggc ttttggtata aaaaatgaag tggcaagaga
     4141 agttgttcgt gaggtaagaa acataacgaa actgccttta gtaataaaat tatcacccaa
     4201 tgcagaagat attgtaggaa tggcaaaagt ttgtgaagaa gaaggtgctg atggagtttc
     4261 attagttaat acttttaaag caatggcaat tgatataaaa aatagaagac cagtatttga
     4321 aaatgtatat gctggattat ctggaccagc tataaaacct atagctttga gaatggtaca
     4381 tgaagtgtgt aaaaatgtaa atatacctgt aatgggaatg ggcggaataa ctaaagccac
     4441 agatgctata gagtttataa tggcaggagc tacttgtatc caagttggaa ctgcaaattt
     4501 tatgaatcct agaataggta ttgaaataat agatgggatt aatgaattta tggatagaga
     4561 aggaataaaa agtttagacg aaataagagg aattatataa ggtagaataa atagtatttt
     4621 aaagataaaa aatatagatt aaataaacaa taattttata acattaacat ggaggaaaaa
     4681 taatatgagt aaagtagatg tagtggatat attaaagaaa agtgatgcat tgttggaggg
     4741 acatttttta ttatcttcag gaaaacacag taatagatac gtacaatgtg caaaagtatt
     4801 aagatttcca gaatatgctg ctgaggtatt aagtacagtt gttgaacaaa taaaagactt
     4861 agatatagac ttagtagtag gaccagctat gggtggagta atagtttctt atgagttagg
     4921 aagacaatta ggaaaagaag ctgtatttac tgagagaaaa gacaatacaa tggagttaag
     4981 aagaggattt gaagttaaaa aaggagcaaa gataataatt gctgaagacg ttgtaactac
     5041 tggtaaatca actatggaga caaaaagagt attagaagcc ttaggtggag aagttgtagg
     5101 tgttgcatgt atagcagata gaactaatca tgatataggt atgcctatat atagtgctat
     5161 aaaacttgat attcaagttt atgaatctga tgagtgtcct ttatgtaagg aaggaaaatt
     5221 accagttgtt aagcctggaa gtagagagtt caaggaatta gggatgtaat aaaaacttaa
     5281 ttatttatag tgttacttaa aaaatgtaaa ttttttagag taaaaatttg taatataaca
     5341 tataacttaa aaataaaaag cagttagcct tataaatgaa ggttagctgt tttttttgtt
     5401 aaaaatatat ttacaaaata agagatagta gcagtacaat ataagtataa ggtaaaagta
     5461 ttatatatta gggaggggta agtatgaaaa atcaattaaa tgaaaatcat aaaaaggttg
     5521 tattttttgg agtaggtgct gtaggagcta cttttgcaga acaatttttt aactctaaat
     5581 atgattttaa aattctttgt gataatgaaa gaaagaaaag atatttagaa gaaggattta
     5641 taataaatgg aaaaaggtat gattttaatt atgtaactaa agatgagtat aaacaagagg
     5701 ctgattttat aattataggt ctaaaatata ataatttaaa agaaaatata aaagaattag
     5761 atggattagt tggaaaaaat acagttataa tgtctctgct aaatggagtt gatagcgaag
     5821 agataatagg agaaagattt ggaattggaa aaatggtata ttcatatgtt accaatatag
     5881 atgcaaagaa aatcaataat aatattatac acactactaa tgggataatt gtatttggta
     5941 acaaagataa tagtgaagat agaaaaacta atataataac tgaagtcttt gatgatgtaa
     6001 atatagaata tattttatca aaagatattc aacgagatat gtggtggaag tacatggtta
     6061 atattggtgt aaatcaaact tcagctatac ttggtgcacc ttatggagtt tttcagagtt
     6121 ctgagcattt aagagaatta gcaaaatctg caatgaggga agttgttgct atagcacaag
     6181 caaaagacat atctcttaca gaagatgatg ttgaaaattc attacataga atactagaac
     6241 attcaaaaga aggaagaaca tcaatgcttc aagacgtgga agctcataga cctacagaag
     6301 tagatatgtt ttctaagaat atctgcaaac ttggaaaaaa atatagcata cctactccta
     6361 taaatcagac tttattttat atgataaaag taattgaaag tagattttaa agggtatttg
     6421 taagaaatat tgataattat tgattttaaa ttttattaat gttatatact atttaagtta
     6481 ctaaatattt ttaactcttc aatgaaaata gttgctgctt ttgacaaagt tatatctttt
     6541 ggaaatatat atgagaaatg tctgttgtag ctgcttccaa gctctatgac agacagctca
     6601 ttgtttaaaa caggtaagct tgtcactaga ttagaaacta ttgtaattcc taaattattt
     6661 ctaacagatt ctttgacagc ataattactt ccaaaaacca tcatactttt tggaataatt
     6721 tctttaacac ttaaaaacat atctaaatag tctctggttc cagaaccatc ctccctgact
     6781 atccattttt ggtcttgaag tttatcaaaa ctaaaatctt ttaataaatg actattgtat
     6841 ggaagtgcta aaaccatctt atcttcaaaa aaatattctt gaataaatga aggggaagag
     6901 caagtgcctt caataagacc aatgtctaaa atataatcct taacatgaga agatacgatt
     6961 gatgtatttt ttataaaaac ttctacatat atgtcaggat acttttgaga aaataatgct
     7021 aaaaaatttg gtagaatata ttctccaata gttaaacttg caccaatctt taagtgacca
     7081 gttatagcat ctgaaacttg gcttacatca tgataagtaa tatttaaaag atttaatatt
     7141 tctttagctc ttttgtacaa tatttgtcca ctttctgtta taaaaatggt tttttgttta
     7201 atagacctgt ttataagcac aactttaaaa taattctcta gattttttat gtgtttactt
     7261 acacttggtt gtgatagatt taagtattcc ccagctttag taaagttttt gtattcaaca
     7321 acagctacaa atgtttttaa ttcttcaaac aattttttgt ctccttcacc taatgaaatt
     7381 ttacattttt tatatacttt ctacagataa ttatactata tataataacc attaaaaaca
     7441 ttaatcatta cgatattata tatttatttt acttatggtt attacaatta tatactattg
     7501 ctatgaagaa aactatatta attataaggt aggtatatta tgttaaagaa aaaaagcagt
     7561 ttgacattaa aaactataag ggaaatatta ccaggactgt ttatatcagt gttagtaggt
     7621 tatattagta tatttatttc tattttaatt cctaaggtag gggcagcatc aatttctata
     7681 tttttaggta tgtttgtagg aaacctattt ttaaatcaaa aggtatttca aaaaggttat
     7741 aaattttcag agacagattt gttatcatat tcaatagtac tattaggggc aacactcagt
     7801 gtatctacct taatagactt aaaagtttca ggaattttat ttataatatt gcaaatgaca
     7861 ataacaataa tagctgcact ttatataggt aaaaaactag gatttgaaga aaactttaga
     7921 tttctaatgg caagtggaaa tgcagtatgt ggttcttctg ccataggagc aatagttcca
     7981 gtggttaatg cgacagataa agaaaaggga attgcagtaa ctattgtaaa tgttactggt
     8041 atattcctta tgtttttact tccaataata tctcaatttt tatatagcca tgaacttgta
     8101 aaaacttcag caatgatagg tggaactttg caatcaattg gtcaggttgt agcaagtgga
     8161 gcaattgttg gagaaaatgt aaaagatttg gctactattt ttaagatagt aagagtaata
     8221 tttctagttg ttgttgtatt agtatttgga catatgaaaa atcagtctaa tagggaaatc
     8281 cttgaagaag aaaaggatga gataaaaaag aaaaaagtaa ctataccatg gtatgtaatt
     8341 ggattcttta taacttgtgc acttttttca atgaacatta taccaaaaga agtatctgtg
     8401 ttgtgcaaag aaataagtaa caaactagaa ataatagctt tagcagcaat tggattaaaa
     8461 gttaatgtaa aagatttagt gaaacaaggg aaagaagttt ctttatatgg tttatttgta
     8521 ggtacagtac aagttgtatc agctgtagtt ttaataaaaa tatttatata gcacaaaaat
     8581 agtatttgta atattaattt gattaattaa aataatatga tactaataat ttataatata
     8641 aataaaaaat gaatttcgat aagtttaagt gggtataaac ttatagaaat tcatttttta
     8701 tttaaggtaa aaatttaaag taaaatatta ttttacataa taataacctt ataaattttg
     8761 aaattactta tctttgtgtt aaaataagta aaatacacat attaaagtta agtttttagg
     8821 caaataaaca aaatgaaaat ataaatatta aggagatttg gtatatgaat gtttatgaaa
     8881 aaggtaatgg agttatacta gaaggagtaa cagattttga cccagtacat atatttgagt
     8941 gtggtcaatg ttttagatgg cacaaacaag aagatggctc atacactgga gttgcaaggg
     9001 ggagaatatt aaatgtcaaa aaagagaatg ataaaatata tttaaataat acgaatttaa
     9061 aggagtttaa cagcatttgg tataattact ttgatttagg aactgattat acagaaataa
     9121 agaataagct aaaaaatatg gatgaatatt tgaataaggc tactgaattt ggatggggaa
     9181 taagaatatt aagacaagat ggatgggaaa tgcttatatc atttataatt tcctctaata
     9241 atagaattcc tatgatacaa agagcgatag aaaatttatc aagaaagttt ggtaagtata
     9301 taggtgaata tgaaggtaat gaatactatg cattcccaac accagaagaa ctaaataagg
     9361 cctcacaaga ggaaataaga gcatgtcaaa caggttttag agataagtac ataaaaagta
     9421 ctacacaggc agttatagag aataatgatg aggtatctga atatacaaat ttaagtacag
     9481 aggattgtag aaaagagctt ttaaaattta atggagtagg accaaaagta tgtgattgta
     9541 ttgcattatt tggtatgcaa aaatatgatt cttttccagt tgatgtttgg gttaagagag
     9601 ttatgcaaga attttatata gatgaagaca tgagtttacc taaaatgaga acttatggaa
     9661 tcgataaatt taaagaaatg tctggatttg ctcaacagta cttattttac tatgcaagag
     9721 aactcggtat aggaaaataa attaaggggg agaaattatg ggagtaatag gaacaatatt
     9781 tctgttctat ttaattatat catatctagc tggagccata atttctgtga ttatattgct
     9841 tgaaaataga gaccctgcaa aaacaatgtc ttggctattg atgtttatta tatttccagg
     9901 tgtaggttta atgatatatg ctatttcagg tagaaatatt agaaaaagaa aattatttaa
     9961 gactcaaaaa ctagctaata atataaaaga aaagaaacta tttgatacat tagaaaagat
    10021 aacagaaata gttgaattag aaaaagaatc tataaaacaa aataagcttt taagagatga
    10081 agaggatggt agttatagaa aaagggtaat aaatatgttg ttaaagactg gtatgtttcc
    10141 ctttactaaa aataataaag tggatgtatt tgtagatgga aatgaaaagt ttaaaagact
    10201 tatagaagat ataagagaag ctaaagacca tattcattta gagtacttta tcatcaaaga
    10261 tagtgaaata ggtagggtgt taaaagaaga acttataaaa aaggctaaag aaggcattaa
    10321 gataagaatt ttatatgatg atgtaggatg ctggagattt tggtttaata gaaaattttt
    10381 ccgagaaatg agggaagtag gtatagaaat tgcagcattt ttacctacaa aatttcctat
    10441 aataggaggg aaattaaact atagaaatca cagaaaaatt gttgttattg atggaataat
    10501 tggatataca ggtggaataa atataggaga tgaatattta ggcaaaaatg ataagtttgg
    10561 atattggaga gatactcata ttaggattaa gggtatttct gtatacatgc ttcaaatgac
    10621 atttctgatt gattggtatt atacgacaaa agaagtatta gtgactaaaa attatttccc
    10681 aagtgtaggg aatgttggtg aaagtatgat acaagttgtt gcgagtggtc cagacagtga
    10741 ctgggaagat attcattatg cttatttttc tgcaatatgt caggctagaa aaaatgttta
    10801 tatagaaact ccatatttta ttccagatga aagtctacta aaagctataa agagtgctgc
    10861 tcttagtgga gttgatgtga gaataatatt tcctaaaatt gcagaccaca agatagtaaa
    10921 tatagcatct tattcatatt ttgaagaaat attaagagca ggtggtaaag tttatttata
    10981 taataaagga tttatacatt ctaaagtcgt aataatagat gataaaattg cttctgcagg
    11041 tacagctaat atggatttaa gaagttttat gcttaacttt gaggtaaatg catttatcta
    11101 tgatgaagaa gttattaggg taatgacaga tgatttcttt gaggatttaa gtcactgtga
    11161 agaacttaat ttggaggtat ttaagaatag aaatatcata caaaaaatca aagaatctgt
    11221 agcaagatta ttttcaccaa tattataaaa aattattaaa aaaggtattg aaaattatgt
    11281 gtgattggta tattataata attgttagca ctctattaga tagagtgata ataaataaaa
    11341 agtatatata taaatctaca tatataagta gtattaattt aaaaataact aaaaacatag
    11401 gcgaactagt ctatacttaa tattttagga ggcgtgaata atgaaaataa gaccattagc
    11461 tgacagagta gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg gaatagtttt
    11521 accaggagca gctaaagagc aacctcaaat agctgaagtt gtagaagttg gaccaggtgg
    11581 aatagttgaa gggaaagaaa taaaaatgga attgacagtg ggagataagg ttatattcca
    11641 aaaatattct ggaacagaag ttaagataga aggacaagaa tacacaatac taagacagag
    11701 tgatgtatta gctgttattg aataaatata gaataaattt attaggaggg atttaaaatg
    11761 gctaaagaaa ttaaattttc agaggaaaca agaagagctt tagaagctgg tgtaaataaa
    11821 ttagcagata cagtaaaagt aacattagga cctaaaggaa gaaatgttat attagataaa
    11881 aaatttggtt ctccacttat aactaatgat ggagtaacta tagcaaaaga gatagaacta
    11941 gaagatagat ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac taaaactaat
    12001 gatgtagctg gagatggtac tacaactgct acagttttag cacaagctat aataagagaa
    12061 ggtttaaaga atgtaacagc aggggctaac ccaatacttt taagaaaagg aatacaaaaa
    12121 gcagtaatag tagcggtaga agaattaaaa aatcaatcaa gaatagtaga aacacaagag
    12181 gctatatctc aagttgcttc tatatctgct ggtgatgaag aagttggaaa attaatagca
    12241 gaagctatgg aaatagtagg taaagatgga gttataactg ttgaagagtc tcaaactatg
    12301 aatactgaat tagatgctgt tgaaggtatg cagtttgata gaggatttgt ttctgcatat
    12361 atggttacag atgtagataa aatggaagca gttttaaatg acccatatat attaattact
    12421 gataagaaaa tatctaacat acaagaatta ttaccagttc ttgaacaaat agttcaacaa
    12481 ggtaaaaagt tattgataat agctgaagat gtagaaggtg aagcattatc tacattagta
    12541 gtaaataaat taagaggaac atttgatgta gtagcagtta aagctccagg ctttggagat
    12601 agaagaaaag aaatgcttca agatatagca atacttacag gtgctcaagt aatatcagaa
    12661 gaattaggtt atgatttaaa agaagctgat ttatctatgc taggtagagc ttcatctgtt
    12721 aaggtaacta aagaaagtac tacaatagta gatggttctg gagataaaaa agctatagaa
    12781 gatagagtaa ctcaaattaa acatcaagta gagcaaacta cttcagattt tgatagagag
    12841 aaattaatgg aaagattagc taaacttgct ggaggagtag ctgttgtaaa agttggagct
    12901 gctacagaag tagagttaaa agaaagaaaa ttaagaatag aagatgctct taatgcaact
    12961 agagcagctg tagaagaagg aatagtagct ggtggaggaa ctgcttttgt tagtgttata
    13021 ccagcaatag gaacactaat tgagagttta gaaggagaag ttaaactagg tgctcaaata
    13081 gttaaaaaag cgttagaaga gccattaaga caaatagcta taaatgctgg tcttgaaggt
    13141 gctgtaattg tgcaaaatgt tgttaattct gaagcagaaa ctggatttga cgcattgaat
    13201 gaaaaatatg taaatatgat agaagctggt atagttgacc caactaaagt tagtagaagt
    13261 gctttacaaa atgctgcatc aatagcaagt actttcttaa ctactgaagc tgctgttgct
    13321 gaccttcctg aaaaagaaga tgcaggaatg ccaggaatgg gtggcggaat gcctggtatg
    13381 atgtaattta aaaaatttaa aatataaatt ttaatataaa gaactatttc gacttagatt
    13441 ttaaactcta agttgaaata gttcttttgt atatatctaa tttttgtata tctataaatt
    13501 caatacacat atttagataa tcagtatcta gtttgtaata atattttaga gtagtattgt
    13561 tattttttag gttatgattt tagatatata ttaattaagg tataattgtt gatagagaat
    13621 aaatcaaatt taaaaggatg cgataatatg gacttaagaa aattacttga agaagttaaa
    13681 aatgaaaata ttgatataga ttttgcgtta gaaaagttaa aagatttacc ttatgaagac
    13741 cttggatatg caaatataga ccatcataga gaattaagaa atgggtatcc ggaagttatt
    13801 tattgtgaag gtaagagtga tgagcatatt ttagggatta ttgataaaat gaatcaaaaa
    13861 agttctaaca tattggggac aaggtgcaga aaagaaactt ttctgaaaat taaagaaatc
    13921 tataatcatg ctgagtacga agaggcgtct aaaattctta agattcaaaa tcatgatata
    13981 gagaatatag gaaaaggtaa aattgtgatt gctacagggg gaacttctga tataccagta
    14041 gcagacgaag cttatcatac agctaagttt ttaggtaatg atgttgatag aatatatgat
    14101 gttggagtgg caggtataca taggctttta aataaaagac ataaaataga tagtgctaga
    14161 gtaatagtag ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg tggactggtt
    14221 gatgttccag taatagctgt tccaacttct gttggatatg gagcaaattt tggaggatta
    14281 gcagcattac tagctatgtt aaatagctgt gcatctggaa tatctgttgt aaatattgat
    14341 aatggatttg gagcaggata tttagcatca atgataaata aactgtaaat acattgatat
    14401 aaattaaaca aaactatata ataaaattta atttctatta tatttttact attaaaattg
    14461 aggagacata tatgagtgaa aaaatacttt attttgatat aataagcggt atatcagggg
    14521 atatgaccct tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt ttggaggaaa
    14581 ttcataaact aagaatgagc gatgaattta atataaacat cagctcgaaa actgaaaatg
    14641 gaatagtagg tacaaaagta gaagtaatta caaaagaaaa acatacacat agaaatttag
    14701 tggatatatt tgaggttata gataaaagca ctctaaacga aaatgtaaaa tctaaagcaa
    14761 aaaaaatatt tatggtaatt ggagaagcag aagcaaaagt tcatggaact acaatagaca
    14821 agatacattt tcatgaggta ggggctatag attcaattgt agatatagta ggagcttcta
    14881 tattagttga cttactttgt gtagataaag tttgtgctag tacagtgtct gttggttcag
    14941 gatttgtaaa atgtgaacat ggaattatac ctatacctgc acctgcaact atagaaattt
    15001 taaaaggtgt gccaattaaa ttaaataatg taaatggaga atgtacaact ccaacaggag
    15061 ctgcaatcat aaaagtcttg tgtgatgagt ttgtagatga gcttgaattt tctccaaagc
    15121 aaattggata tggtataggt cataagaagt ttgaagtacc aaatatactc agaactgtat
    15181 taggtgaaaa aaaaaagaag aattaattta tgaaattagt gcaaacattg atgatatgtc
    15241 ttcagaaatc tattcttatt tgtatgaaaa aatattagat gagaaagcat tggatattta
    15301 tacagagagt atttatatga agaagaatag accagcaaca aaagtatcaa ttttatgtaa
    15361 tgaatcagat ttagaaaagt ttattaaaat attactttta gaaactagta cttttggagt
    15421 gagatataat gcatataata gagaaatttt agagagaaaa tttgtaaaat tagatacaaa
    15481 atatggagtg gttacagtaa aactagggta ttataaagga aaactagtca aggttactcc
    15541 agaatataat gaatgtaaaa ctatagcaaa aaatgaagat attcctataa aaaaagtgtt
    15601 tgctgatata aattttagaa taaaagaaga atttagtgaa aacttattga cataagtaat
    15661 aaattttgat aaattaatat tcagccttat taaataaaag taaacagaaa aaaatatata
    15721 aaatataaac tcactcgtat atgctcagaa atatggtctg agagtctcta ccaagatacc
    15781 gtaaatatct tgactatgag tgaaattatt ataccaaaag acctagttaa gtaaatgtgt
    15841 aggctgagtt atactcccta tgtgttggct ataagataaa ggtattttgg aagcctgtat
    15901 agtaatttca cttttgctag aaattatgga agttactata caggtttttt gtgtaaaaag
    15961 atttaatata atttagactg tattgtgtta aaataaatat ataaaaacta tattatacaa
    16021 ggagagatgt gaatttatga aacatgaatt agtacttgta atagattttg gaggacaata
    16081 taatcagttg atagctagaa gagttagaga aaacaacgta tattgtgaaa tacttccatg
    16141 tactgcaagc atagaaagaa taaaagaaaa aaatcctaaa gggataatat ttacaggcgg
    16201 tccaaatagt gcgtatttag aagattctcc aacaatatca aaagaaatat ttgaactagg
    16261 agtaccaata cttggaatat gctatggtat tcaaataatg tctcatgtat tgggtggagt
    16321 tgtaagaaaa ggtaataaac aagaaaagga atatggaaaa acatctataa cttatggaaa
    16381 atcatcatta tttgaaggaa taactacaaa tagtgtatgg atgagtcata cagatttaat
    16441 agaaaaagtt ccagaaggat ttactatagt tgcaaatact aatgattgtc cagtagcagc
    16501 tatggaaaat gtagaacgaa acctatatgg agttcaattc catccagagg tagaacactg
    16561 tttggaaggg gacaagatac ttacaaactt cttatataat atttgtaaag taaaaggtga
    16621 ctggactaca gattcattca tagaagataa aataaaagaa ttaaaagaaa aaataggcga
    16681 taaaaaagct ttatgtgctt taagtggagg agttgattct tctgttgctg cagttcttat
    16741 tcataaagca attggggaca acttaacttg tatatttgtt gaccatggtt tacttagaaa
    16801 aaatgaagga aatgatgtag aaagaatatt tagagagaaa tttgatataa atttaataag
    16861 agttaatgca gaagatagat tcttatctaa attaaaagga gtttccgaac cagaagctaa
    16921 gagaaaaata ataggtgaag aatttataag agtatttgaa gaagaatcaa ataaattagg
    16981 aaaaatggat ttcttagtac aaggaacaat ctatccagat gttatagaaa gtggacatgg
    17041 aaatgctgct actataaagt ctcatcataa tgttggtgga atcccagaag atgtagactt
    17101 ccaagaaatt gtagaaccat taagagagtt atttaaggat gaagttagaa aaataggatt
    17161 agagcttggt atagaagaag gattaatatt tagacatcct ttcccaggac caggtctagg
    17221 aataagagtt ataggtgatg taactaaaga aaaatgtgat atattaagag aagctgatgc
    17281 tgtttatatg gacgagctta gaaaagcagg tttatacaga gaaatatggc aagcttttgc
    17341 aacattacca gatgttaaga ctgttggagt catgggtgat gaaagaacat atgcttattt
    17401 agttggttta agagcagtta cttcttctga tggaatgact tctgactggt ataagatgcc
    17461 ttatgatgta ttagaaagaa tttctaatag aataataaat gaagttgatg gagtaaatag
    17521 agtagtttat gatataactt ctaaacctcc tggaactatt gaatgggaat aaaatataga
    17581 atgccagaaa tccacatgaa tacaggattt gaggtctctc ttacttctat attatactgg
    17641 tttgatacta tcaatatttg ccagtatctt atggattaat aagcaaaaaa catcctgctg
    17701 aatggtaaat tcggcggggt gtttttattt tgttttgaaa ctcctttgct ctgaaactcg
    17761 aagtatttaa cactgatgat gttgttatct tttagttcct tttgtgtcat gctctgaaag
    17821 acaaattgat acaataaaaa agtaatttct taatcattga tggagatatg tatttatgtt
    17881 actcttgatg gtaatttgag taatattgtt catagctttt tatattcact taattagaaa
    17941 accatttggt ataatcataa ataaacttga tgatataata gaaaagattt ataattatta
    18001 gaagtatata aaagtatttt atgtctatga aatgtatgtc tataaaacat atagatgact
    18061 tttgtatatt aatgtatgaa tattgatatt atagaagaat tgagttgtca atgatactaa
    18121 atcaaatatt tatagaagat ggagggatta tgagaaataa aataattata ggaattactt
    18181 tttcacttgt attactaatg tctggatgtg cttcaaaaca aacagcagaa ataaacgtat
    18241 tagcaactac tgatttacat ggagttatac cttatgagtt aacttcttat gtaaaagaag
    18301 aaagaaaaaa agataaaaat ctaactttag tagatgcagg agattttttt gatagtggag
    18361 aagtgtttgg ttctagcatg gatagttatt ttagtgctag atataaagat ggtgataata
    18421 acactgaaag ttatattgag acgcctattt caaaagatat gaaagaggta ggttatgatg
    18481 ttgcagttct tgggaatcat gaatttatat ccaacaataa attttatcta gataatatga
    18541 tatctgattt tgagaaacaa aaaataaata ttttatcggc aaatacgtat aaaaaaaatg
    18601 gagatagtta tacaaaacca tacataataa aaaatattaa tacacctgaa ggaaacgtaa
    18661 aactaggaat tttagggctt actataaaag aagttggtga gaggaaacag tggaaagatg
    18721 gaaaacttgt tgactctaag tctttagaac taaaagacca agagggctac aatggtgaat
    18781 tgtatatgaa tgatttagta gaagatgcta aaaagtgggt tggtataatg gaaaaagaga
    18841 agcctgacat tatagttgct gtggcacata caggtgagaa acctaagaaa cctagaaacc
    18901 caggaaatag aatacaagac ttagctcaac aagttgatgg aattgatgct atagttgcag
    18961 gtcataatca tgttcaaatt gagcaacatg attataaaaa taaatcagga gaaaatgtta
    19021 tagttactga gccaggaaag catggggaat atatttctaa gattaatttt aaattagaaa
    19081 aaaacaatga taaatggaat atagttgaca aatctagtaa attaattaaa tttgaaaaaa
    19141 atcaagaaag tgataaattt ggagaattta tttcttcaat taataaagta agtaaagata
    19201 agaaagaaat aagattatca gaagtaaaga ctcctgaatg ggataaaata tatgttttta
    19261 aatctggaac atctgcagat gatatatata aaacaatagg atataaatgg agaaatatat
    19321 catctccaaa gggtgatgac atggtgcaga ttgttgtaat gaataataat aagcctgtta
    19381 gctatatata tggtgaagct gaagttatgc caattaatat aaaatgtgat gaatcagagt
    19441 ataaaaataa tgttgtaact atttatccta ataaaaatga taaatttaaa attgaaaaag
    19501 gtaagcaaga atatagtatt aatttaaaac atatacaata gaaactttta tcttatttaa
    19561 gtttagattg tacttaaaat aaagtgtgta ttattttaaa aagtgatgat aattaaaatc
    19621 acaacattaa ggaatagtac acacttttta tgctaaaaaa tagaatgtta aaaaattaaa
    19681 cctttaaatt aaagtacatt gcttatatta tccaatatta tttccaaagg ttgtgtaata
    19741 tttttatcct tatgtaatat tgctaacatt ttattttcca tatctatctc atctaattca
    19801 atcaatcgca attttttatg taagagtgaa tctaataata aattttctgg caaaatggtt
    19861 atacctaacc ctgcttctgc agctttaata agtgcaaaag aattaacact ttcccaaaca
    19921 ggataagctt tttggtttga aagagataat acactgtcca aagtatcgcg aatagcactt
    19981 ccttgttcac gtaacaataa cggaaacgtg cataactgtc gtagagataa ggtttgttca
    20041 gataaaggaa aagttggagc acatgcaaca cataacttat aggaacctaa taaaattgta
    20101 cggaatgccc cctgtggttc tataccttcc caaaaacata tatcagcttc tccacttttt
    20161 aaaatatcca tagctgaatt agcactttcg acacgtacat tcactttaag agatggaaat
    20221 gaatttttta gtcgacttaa aattggtgga agtaaaaaag aggcaattgt aatgctagaa
    20281 acaatgttta taggtgtaca ttcttctaaa cgactgattt ttttgtctaa attgcgacaa
    20341 gcagttaaga tactacgtgc ttcatctaaa agagaaatgc cacagcgagt aagacatacc
    20401 cctcgtggta atctgtcaaa tagtgatgtt tcagcttgtt gttcaagttc agctatagca
    20461 tgagatacag cagattgtgt gatatgtaat ttttttgcag cacctgtaaa agtgcctgtt
    20521 tgttcaattg cttctaaaat ctctaaatat cgtatgttta tcataataaa tatcctcact
    20581 atacatgaat aaaattaatt aattccatta aattctatca ttttacagat tgcaaagcaa
    20641 caggtatact gaataatatg aagtgaaaag aaggaggaat attttgaatc aaagcagaaa
    20701 aatatatttt tggcttttaa ttattaactt gtttattagc acattcactt ttggcggagg
    20761 gtatgtagtt gtacctatga ttagacgtta ttttgtaaat caaaagcaat tctttacaga
    20821 agatgatttg atgagtatgg cagcagttgc acaatcaact cctggagcta ttgcaattaa
    20881 tttatctgct ttggcaggtt atcgagtagc tggtacttta ggggttgtca ttagttgtac
    20941 agcagctata atacctccac tcgttattct tggattagta tcagcatttt acaccacttt
    21001 tatatctaat gccattgtaa ctgcagtatt aaaaggaatg caagctggtg ttgctgcact
    21061 aattgtggat ttgattattg acatgtgctc tatgatatta aaacaacgtt ctttgttttt
    21121 atcaactatg atacctattg ctttctttgc aaactttgtg atgagtataa atgtaatttt
    21181 tatattatct gtttgctgtt ttgtatgtgt aatgcaagtt ttctataaga agagggaaaa
    21241 taaatgaata caatttggaa cttatttttt atatttttaa aaataggctt gtttagtatt
    21301 ggtggtggtt atgcaatcat acctttaatc caagagaaag tagtaaatca aactggatgg
    21361 ataagtgaga aaatgtttac agatattata actatttcac aaatgacacc agggccactg
    21421 gctgttaata cttctacttt tgttggactt caaattggag ggttttgggg agcagttgca
    21481 gccactttag gttgtgtatt atgtggtgta gtaatatctc tatttttata taattttttt
    21541 caaaagcatc agaactctac ttatatattt gaaatattaa atggtctaaa atcagcttca
    21601 ttaggtttaa ttatctcagc tgcggctaca attataatcc ttgcattatt tgggacgaat
    21661 aaagtgtact tcaattctat atttgagatg ctaaactgga ctgcactaat aatctttttt
    21721 gttatactac tgatagtacg taagtggaaa attaatccta ttgtgattat aataataagt
    21781 ggtatactag gaatattttt ttatttataa gtttttggta agtaaaggat atctattttt
    21841 ttatatctta aatccaaata gccaataaag taaggctatt tggattttta ataaattctg
    21901 aaaagataat cagaaaccca tataacattt aaattatatc actggttttc tacaaaccaa
    21961 tcataagctt cttggcaaaa tttctcacta tctatatagt aactattatg aacattatta
    22021 atttcaacaa gtttcttttt acattttagt gaattataat agctatcaaa atattgggtt
    22081 ttaaaatatc tatcatgaag tgttttaaag acaagagtag gtatgttgac tccatctttt
    22141 atcttactat cttctgcaat aaagcattct aacattgtat taattatctt tgtactattt
    22201 ttccaaatat gaggattttt cttttcaaat attttataaa attcattatc atagacttca
    22261 ttataacctt tatatttagg tggttttact gttatgtgaa aattaggaag aaacttaaag
    22321 aaaaaatata ataattttta tgttctgttt tatatattat tcatatttta tcacagataa
    22381 tatactaaag ttaatttttt tattgtacaa ctattataat aattcttcta taatataaga
    22441 cattctataa ttttatattt aagttcatgt gtatttaatt gtttttatat aaaatgtcca
    22501 gtaatactat ttggatttat ttttatttca tcaggagttc cagaagcaac aatttcccct
    22561 ccatttattc cactgtctgg acctatatcg ataatccaat cagaagcacg tataacctct
    22621 gtattgtgtt caataattaa aagtgaatgc tgttcattta caagttttct aaaagtaatc
    22681 aacaaatttt ctatatcttt aaaatgaagt ccagtagtag gctcatccaa tatgtacagc
    22741 attcgttttt tagtttttgt ccctaattct tttgctaatt ttatcctttg agactctcct
    22801 ccgcttaatc ctaatgtatt ttgtccaagc tttagatatc caagtcccac ttcatctaat
    22861 aaggagagtt ttctatatat atcagagcaa tcactaaaaa atgtttttgc ttctgctact
    22921 tccatgtcaa gtatatctgc aattgttttt cccttgtatt gtatagacag tatttctttt
    22981 tgatatctct taccttgaca ttcattacaa gttacccatt ggtctgccat atattgtaga
    23041 ataatttttg ttttacctaa tccttgacag tttgggcatt gccccttttt gctattaaag
    23101 ctaaaatagc tttcatccaa taatttttgt tttgcttgtg gagtttcagt aaatagagaa
    23161 cgaatttcat caaaaatatt gatatatgta gcaggtgtag aacggctaga ttttccaata
    23221 ggtgtttggt ccattaagat aaaatcgtca atagcatcaa atccagtaag tgcagtataa
    23281 tttttatttg ggatagattt ttgtttcaat ttttcttcta gagcaggaag taaactgtga
    23341 aatacaagag aactcttacc agaaccacta acacctgtaa tagagcacat acagtttaag
    23401 ggaatttcta catcaatatt ttttaagtta tttgcatggc atccctttaa gcttaaccat
    23461 ttacttggta taattttatt ttgtgaactt atcttattgt atgtactcaa atatttgcct
    23521 gttatagaat ttggattttt tataatttct tgggttgtac cttctgcaat gatgaagccc
    23581 ccttttgtac cagcatttgg tcctacatca ataatataat cagcagacag tattgtatcc
    23641 ttatcatgtt ccactactat aacagtatta cccttgtctc ttaattcttc tatcattttg
    23701 atgattagat tatggtctct tggatgtaaa ccaatagatg gttcgtctaa aatataggtt
    23761 aatcctacta attcacttcc tagctgactt gataatcgta ctctctgcag ttctcctcca
    23821 gataaagttg gagctgttct atctgttgat atatagctaa gacctacctt taatagataa
    23881 ctaactcgaa tttttaattc tgttaagata tcatttacca aagatagctc atttttttga
    23941 agttgatttg gtaattcaca caaccaattc cataactgtt ctatcgtcat ttttgttagt
    24001 tcagggaagc gataaccttt gattgtagtg aatctagcct caatgcacag caattcgcca
    24061 ccacatgtat tgcaaggaat tttgttcata tattgatgca aggtattatt ttcagaacta
    24121 gattctctaa aaagtctttg aatgtggttg attgctcctg aagcaggcct tctaatcttt
    24181 gattctcgtc cctttgattc aaagctaaat tcatatatct tatcgtctgt accatataag
    24241 attgcatcta taaaatctct tggcaattca ttccaaggta tatctaggtc tatattttta
    24301 tcttttgcta ttgcatataa ttcacctacc atccaattac catttggctt tttccccctc
    24361 aattttccaa aatagtaagt agctccatct aatattgaaa tagttgggtc ttttacaatt
    24421 aattgaggat taacttgata ggtaaaaccc aatccattac aatcgtgaca agctccagta
    24481 tgggagttag cattgaaggt tgaagtattt atcttagaaa aaatagtttg gcaatgtgga
    24541 caataatttt ttgtattatt ctttttttct aatggagttt tgcaatatgg acagtaactt
    24601 tttccgattg atacaaataa cattcttaag taatcccaaa tgcttgtaag agtaccaaca
    24661 gtagaacgag gattttggct tccttttttc tgttcgatag ctatagtagg ggttaatccc
    24721 tgaagtaaat caaattccgg tttttgaagc tgttctgaaa gttttgctcc attacctaga
    24781 ttttcaaaat aacgtctttt tccttcttca taaatcgtat cgaaagctaa agaagatttt
    24841 ccactaccac ttacacctgt aataacagtt atttgatttt ttggaattgt aacctcaaca
    24901 tcctttaaat tgttttgcct tcctcctttt agatagatag agcttttatt atgacaaagt
    24961 gaatttttaa ttgatttatt tattgctttt gcaaatttga atttcttttc tagttcaaga
    25021 ggattgccta attcttttat ttccgaaata cctaatattt ttaactgaga aaagattgat
    25081 ttcaaataat cttcaggcaa tttttctatt aacaaattaa ggtcttctat ttttatattt
    25141 ccgcttgata atatctctgc catttcatct aataaatttt tttctgctct tgttaattga
    25201 ttgtattttt tggaacttaa ttctgtccat aaatcccatg gaagcaattc taaaccaatc
    25261 atattattaa agtcatgtag aagattaccc tttacagaat gccaaccttt ccaattttta
    25321 ttatatccaa agtcatctgg atgagcatgt ccttgtcgac atcttatcca ggcttctgaa
    25381 ccagttaaaa aaccatctgt ttttccgaca tcatgagtgt caaaattaat ttgaaaataa
    25441 tttttttgta tgtcatctat ttgtgcatct attcttatcc atctctttgt tagagtatca
    25501 tagtattcta caagccagtg gtcttcataa gtaagctcat tttcaaaata attagcaaaa
    25561 cctgctctca ttctagcctc aaaacctcga tatcttagga gcgatacaag aagtaaagaa
    25621 taatctctgc acattccaac aagcctttgt tttgggtttc ttggaatagt tagtgggtta
    25681 gaatttattt caaatattct ttttaacatt tgaggaattg tacgaagcaa ttcctcatca
    25741 gattgttgct tattaaagga tataccatat aattttcctt ggtctccatg aattactagg
    25801 ctttgaacca cttttaataa ctcaattgga gtctttggta aatcttgata tttattttca
    25861 aattttttag gatttgtgat gatgctttga tttttatagt tatttatttt catttatatt
    25921 tcacctctct aaatggatga tataccctga catggtgtca tatgcaagga caatttagat
    25981 tttttggcca aaatttaaaa ataaataaaa aatgatacag tgataaattg taatacaaat
    26041 atagtttcaa attgcatata atataattat ttaataaaac cattatctat ggaatgttaa
    26101 tgaaatgtta agatggcatt cttgaatatt aagaaataga atactataat atagtgtgaa
    26161 ggattcttag taaaaaaatt tgtaagtgtt aatatacatt aaaaaatagg tttataattt
    26221 ggaggaaaaa aatgaaatta tcaaggtata agaaaaatag tttagttctg tctataattt
    26281 tatgttcact tattatttca atccttgtaa ttactgtttt taataccgta aaactacaca
    26341 atatatctaa taaacaatct aaaaactatt taaacgatgt atctacacag attgtaatga
    26401 atgttgattc taaaatcaaa tttattttat ccgacttaag aataatggca gattttatta
    26461 agcaatatga aggtgatagt cgatatgagt atttatctaa aagaaagact agttataagt
    26521 attatgatgt tggaataata gatttagaag gtaaagctga gtttttaagt ggaaaaaagt
    26581 ttgacttaaa gaatactgct tcctataaaa aaggaattga aggtaaagaa tactcagaag
    26641 taatagagtc aatgggtttt gttctttata gtgtacctat atttaacgat aatcatgaag
    26701 tgtctagtat tttaataggt gtatccaata aagaaagcat ggatgagatt cttaatatag
    26761 atagctttaa tggagaagga actattgaaa ttattaatag tcagggaaaa actcttttca
    26821 taggaaaaaa taatgagtta ataaaagacc ttagctataa gtataaggat tctgcagatg
    26881 aaccttgggc acaaaagatg cttaatgatt ttaaaaatga taaatcagga aatattacaa
    26941 taacctcatc aaaaggtgta caatgcttat taacttatca tccaattaca agaggtttaa
    27001 atgattggca ttttcttctc atagtacctg aagatgttgt acttggtgaa cttaataagt
    27061 taaattcttt tacaataata atgacattta taactacatg tattattgga attataacat
    27121 taattttata tattataaga agaaaatatg taaatcaaat agaaaatatt gcatatttag
    27181 atagtataac aaatggtata aattcaacta agtttagtat gctagtcaaa cctttaattt
    27241 cagaatcacc tgatagtact tatatgatga tagcaatgaa tataaaagat tttaagctaa
    27301 taaatgattg tttcggtagt gaaaaaggaa atatgacact aaaacatctt tacaatattt
    27361 tgtataaaaa cattgatgaa aatgaagaat ttgtgtgccg tcatgatgca gaccttttct
    27421 atttgcttat taagaataga ccagtgttag aagccctgga gttattatat aaaatagaag
    27481 ctgacacaaa ttattttaat aaaaataggg aaaatccata ctttttaaga ctatcagtag
    27541 gtatttacac aattgaaaat cataatgaag atttaattac aatacaagac catgcaaata
    27601 cagcaagaaa aagctttaat aaaactcatc aaagtgattt ttctttttat agtgatatag
    27661 aaagaaaacg attaatatct gaaaaagaaa tttcaaattt aatggaaaaa gcccttgaaa
    27721 ataaagaatt ttttatgtgc ttacaaccta aaattgatat aaaaagtgga aaaattagtg
    27781 gagcagagtc tcttgtgcgt tggaaaaatt cagaaaaggg cataatttat ccaagtaatt
    27841 ttattcctct tttcgaaaaa agtggattta tatgtaagtt agatttatat atactagaag
    27901 aaacatgcaa attgatttct aagtggataa aagaaggtaa agaagtgttg agaatttctg
    27961 ttaatgtatc acgtcaacat ttgaatgata aattgttttt agaaaaatat aagcatatat
    28021 gcaataaata caatgtacca acatgtctga tagatttaga acttaccgaa tctattttct
    28081 tagaaaatcc agaagctata gatataatag aggatattca ttctaatgga tttaaatgtt
    28141 ctatagacga ttttggattt gggtactcat cattagggat attaaaggat tttaaagttg
    28201 atataattaa gttagaccgt tcattctttg ttagcaaaaa taatatagac cgtggtaagg
    28261 tagttattaa gtctattatt gaacttagca aaaggctagg tatgaaagta actgctgaag
    28321 gaattgaaga attagaacaa gttgagttcc taaaaaatgt aggatgtgat tatattcaag
    28381 gttatgtttt ttctaaacca ttattaattt ctgattttga aaattttgca tatgaaaata
    28441 ataaaattaa aaatatccat tattaaatgt acaagataaa aaatttaggc aagttatata
    28501 attaaaaata taacttgtct tttttattta tatatttaat aaaaatatat ttgcagattt
    28561 tgtaaaaata attttcaaat gttgtaagtt gtggctaaag ctttattgta ttattctgaa
    28621 tatagaaaga tagttactgt cttagataaa aactgaaaca tacataataa tataaaattt
    28681 gctatattaa taactctaat tattttggaa ggagggaaaa tatgataagt gggagaatgt
    28741 tactcatcat gaattttcta aaaggaaaaa ataagacaag ttacaaggat attagcaaac
    28801 aacttagtat agaagaaaga aaagttagat atgatataaa taatttaaat attgttttgc
    28861 gttcattaaa caagtcagta atacaaaaga tgaacaaagg tgtgttaatt attcctgatg
    28921 acttccaagt agtatgtata aataataacg aatatgtatt ttccttaact gagcgagtat
    28981 ctattatgaa aattatatca atgtttaaaa tagataatct aaacttagaa aaattgagta
    29041 aagaatttca ggtttctaga agttcaatta aaaatgacct taatgttttg tcaagtgaat
    29101 tagaaagaaa ccaaataact attacttatg ataaatcttt taaaataagt ggagaagaag
    29161 accttatata taagcaaaga ttgaacatat taaaatcgta ttcttatctt tttggtaagg
    29221 aaaaatctga tttaagtgta tttgagaagt atttagtgaa tgtaattgaa aaaatattca
    29281 aagggattta tttgattgat atatataatt ggtctatgaa tttattattg caaatgggat
    29341 ggactttaaa tgatgaatct tttacatggt atgtgtcaaa cattttcctt tttacatggt
    29401 atatacattc agatgaaaaa catcctttag aatctgcttc attaagtgag ccatgttttg
    29461 aaaataagtt tgaccaagaa tttgaaacaa ttattcaaaa aaagttgact aaggaacaaa
    29521 tttttctgtt aaagagtttt gtatttttta caaataaata tgcaagttta aacgaggata
    29581 tggatttgat tagtacagaa attatagttc aagacctaat ttacaatatg tcagaaatat
    29641 tagagattag ctttaaagaa gatatgattc tttataaagg attactcaat catattgccc
    29701 cattgataga gagaatcaag cagaatatac agatttatga ggaattactt catgtaatac
    29761 cagataaata tcaatatgta cttgaagcta ctaaggtttc tattcaaaaa aacagagttt
    29821 taagtcaaat aaagaatgaa aatgaaataa tacttttggc aatacatttc ttaggaagta
    29881 tacatagaaa tgagaggaat aactacaaaa atatattgtt agtttgtgga tttggatatg
    29941 gtgtaattgc tatgttaaag gatagtttag taaatgatta ccagataaat atagtagaga
    30001 gtattccatc atataagtta caagattata aagaatggga aagtattgac ttcattataa
    30061 caacatcaaa gatagtaata gacttgccta aaacaattat tgagataaat ccatttttac
    30121 aagaagagga ttatttgaag tttgaagaaa aagggataaa aagaaagaac gtcttaacta
    30181 attatatgtc aataaaaaaa aggttggact ttttagaaga agatgctcaa aaaagagtat
    30241 taagtattat atgtcaagaa cttggataca gtgatgagag aatattattt agacaactta
    30301 atttaagtga tttaataggg attgatacca taaggataat tgatgaagat atgaaatggg
    30361 aagatgctgt aaaaattagt tcaaacatac ttgctaattg tgattttgta tcagcacaat
    30421 atgctgagaa tattatagat attttaaaaa atgtgggttt ttatgctgta aaagataacg
    30481 aatttgcttt actacatggc aatgatagtt cattagtcaa agtaagttcg ataagtttat
    30541 taatatgtaa agaagcagtt agttttggag ataaaaaggt taaaatcata ttttgtctag
    30601 caagcagaga taaaaaagag caaattcctg caataattaa tctaactaaa atggtttata
    30661 agactaattt tattgctatg ttagaaagtg caaagacaaa agaagaagca gaagttttaa
    30721 ttcataaata tgagaaggag gtcactgtat gaataaatta gttaatgtaa aatgttcatt
    30781 taaaatacat gctaattcaa aagaagaagc tattgtatcg ttagttgatg tagtatcaaa
    30841 agaaggatat ctaatagata aaaatcaatt tttggaagat gttttaaaga gagaagaaac
    30901 attgtcaact tatattggac atggtatagg tcttcctcat agtcaaagtg taggtgttaa
    30961 aaactcttgt attacaatag gaaagcttga tattcctatt gaatggacag aagaaggaga
    31021 aaaagtggat ttaatatttt taatatcagt aacaaaagac aatgaaaata atcttcattt
    31081 aaagatttta tcaaaattag cacgtttatt gatgcatgaa agttttagaa atcaaattag
    31141 atgttcagat gaacaaactg tttataatct tataaaagaa aaaatagagg aggaagatta
    31201 aaatgagaat tttaaagttt ttgcaaaaac atcttatgac agcaacatct tatatgattc
    31261 catttgtggt tgcaggagga atattatttg ctttatcagt aactctaagt ggacaagctg
    31321 ctgttcctga aacaggctgg ttggctaaac tgaatcaaat tggagctgct ggcttggctt
    31381 tattcattcc tatattaggt ggatatatag catttagtat ggcagataaa ccaggattag
    31441 ctccaggtat gataggtgca tatttagcaa aagaagtaaa tgcaggtttt ataggtggta
    31501 tcatagcagg atttatagca ggatttgttg ttttacaatt aaagaaaatt aaattagctc
    31561 ctacaatgag aactttaggt tctatattta tttatccttt gattggtaca ttgattacag
    31621 gtggtataat tgtattttta attggagaac ctatagcttc ttttatgaca tggatgacaa
    31681 actggttaaa tggaatgtct ggtgtttcta aaattccttt aggtggaatc ttaggaggaa
    31741 tgatagcctc agatatgggt ggtcctataa ataaggttgc tgctactttt gcccaaactc
    31801 aagttgacac acttccatat ttaatgggtg gagttggcgt tgcaatttgt atacctccaa
    31861 ttggtttagg attagctaca ttattgtttc caaaaaaatt tagcaaggaa gaaagagatt
    31921 caggtaaagc ttcgctatta atgggatgtg ttggaattac agaaggagct attccatttg
    31981 caacatcaga tccagttaga gtaatacctt gtattatggc tgggtcgatt ataggaaata
    32041 ttatggcttt tttattagga tgtttaaatc atgcaccctg gggaggtctg atagtattac
    32101 ctgtagttga caataggttg gggtatattg catcagtatt aactggagca gtagttgttg
    32161 cagtattaat gaaattagta aaaaaagacg tgaaagaaga tgaagaaatt gaagaggatt
    32221 tagatgaatc aattgaatta atatttgaag aattataaat ataaatggaa ggaatttgta
    32281 taaaaaagta aatataaaac tataaactta aaggagataa gaatatgaaa attgtagcag
    32341 taacatcttg tcctagtgga gtagcacata cttacatgtc ggctgaatca cttgaattat
    32401 cggccaaaaa atttggtata gaaataaagg ttgaaactca aggaagctct ggaatagata
    32461 atgaattgtc acttaaagat attgatgaag caacttgtgt aatattgact aatgatgttg
    32521 agattagaaa tatggaacgt tttaagggaa agaaggtagt aagaatgagt gtatctgaga
    32581 taattaagaa atctgatgca ttaattaaga aaataaaaga tacttttcaa taaaaattga
    32641 tttattttac atataactca aagatgtttt taaagaaatt tttattaaaa gcatctttga
    32701 aaatatctta aaaatagaat aaaaggagat atagtcatga aaaaattacc aataaagact
    32761 gtcgtacaaa gattactaga cttacaagaa gaggggaaaa gtgcaactct tttaggaatt
    32821 ggtcctatgt caccaaactt gttacaagca agttttgaac tagcaaaaga tgatgatttt
    32881 cctttaatgt ttatcgcaag tagaaatcaa gtagatgcag atgaactagg tggcggttat
    32941 gtaaatggtt ggaatcagga aacttttaca aaagatatta agaagattgc cgacaaagtt
    33001 ggatttgatg gtttatatta tttatgtaga gaccatggtg ggccatggca aagagataaa
    33061 gagagaaatg accatttgtc tgttgatgaa gcaatggagt taggaaaaaa atcttattta
    33121 gcggacattg aagcaggttt tgacctattg atgattgacc ctacaaaaga cccatttgaa
    33181 attggaaaag taattccact agatgtagta ctagaaagaa ctgtagaatt aattgagtat
    33241 tgtgaaaacg agagaaaaag acttaatcta ccagacatag gttatgaggt tggtacagag
    33301 gaaaccaatg gaggtcttac ttcaactgag acgtatgaaa catttataac tagactaaag
    33361 gttgaacttg ataatagggg tttgccaatg ccaacattta tagttggtca aactggtact
    33421 ttaactagaa agacagaaca agtaggaact tttaatttta gaaatgctta tgatttagct
    33481 caaatggcta agaaatatgg agttggttta aaagagcata atggcgatta cttagatgat
    33541 gtaactttat tagaacatat accatctcaa attattgcta caaatgtagc tcctcaatat
    33601 ggtactgaag aaactagggc atatttaaaa ttagcagaag ttgagtgtaa attagaaaaa
    33661 gagggcctag ttgataaaac ttctaatatc agacatgttc ttcttgttaa tgcaattgaa
    33721 tgtggacgtt ggagaaaatg ggtagtagga gaacaaaaaa atctaactac tgaagagata
    33781 tttaagaatg aagttttatc taatgaaata ctagatattg caggacatta tacatttaat
    33841 aatgatgatg ttaaaaaaga aatagaagtg ctttatgata atttaagtaa gaataatatt
    33901 gatggtcaaa gatttgttgt agaccatatt aaacgtcctt tgcgtgatta tgcagaatgc
    33961 tataacttaa aaggtgttac tacgaggatt ttaaataaat aataggactt atagtggtga
    34021 gtattgttat actaggtatt tatagttata attatactta ccactattaa atttaggagg
    34081 actaatattt tgattaattt aatttttgat gtagatgata ctttatataa ccaattaacg
    34141 cctttttaca ctgcatataa taaagtgttt tcttcaataa aggatatttc tattgaagat
    34201 ttatatatga gtagtagaaa gtatagtgat gaggtatttc atatgactga aaatggagaa
    34261 atgccaatca aagaaatgca tatttatagg ataatgaaag cttttgagga gttgggaaat
    34321 tctattacag aaaaagatgc acaaagtttt caagatgagt atatatatca gcaatcacaa
    34381 attacactaa taccagaagt tgagaggatt ttaaattttt ctaaagaaag aaatattaat
    34441 ctaggaatta taactaatgg cccatcaaac catcaaagaa tgaagttaaa gcagttaaat
    34501 attgaaaatt gggttgataa aagtaatatt tttatttcta gtgaggttgg attttctaag
    34561 ccagatacta atatttttag agtagctgag aatgttatga atttagatag agaaaataca
    34621 tattatgtgg gtgattctta tagaaatgat gtattaggtg ctaaaaaagc tggttggaag
    34681 agtatatggt tgaaccatag aggacatgag gtagaagagt tgttttataa acctgatttt
    34741 gtaattttgg aacataagga tttgatatct ttatttataa aaatatgtag ttataaaaat
    34801 atgtagtaaa aaaatgttaa taatagaatt gtataaaata attaataata actatataaa
    34861 attatatgtt tagattgtga gcaatctatt ttatgttaaa aatagattat ttagaagaag
    34921 gggaaatctt aaaaaaattt gaatatcccc tttttattat ttaattaaaa tgaaatagtt
    34981 agaaatatat tttatttagt ttagtcaata acattttggg attctttata actacatgtt
    35041 aactgttgaa taggcttatc atatggttta acccatcctt tattaatact taattctgta
    35101 agtgctgtgt gaccttctac catttgagtt agtgaagctg aatatatagc tcttaattca
    35161 ggtgtactac ttgttaatgc agaattaaga tacatattgg cagatgcttt agcagatgaa
    35221 agcatactta acattattac tttatcgtct atataagtat tgttcttaat tagattttct
    35281 atcatatttc tcatgttatc tatacctcct cagaaattag tatttcattt tcaactataa
    35341 attgttgtat agctttaatt cttccttctg ttgctaatat actagattct gattgtcttt
    35401 ttaggtcttc atctgatatt aacatattta ttgctctttg cataagtaat ccgtcagact
    35461 ccatttttat aactgctgct aaagatattt tttcagtttc tgaaagtgtt ctcatttaat
    35521 catctcctat attgtaattt atgctttaat agtatgtctt tcttttcaag gaaataacca
    35581 ttacactttt ttacagtatt ttatattctc tttatatcaa agtttagtca acttaatata
    35641 ttaatataat tttgtaggat atttatcatt aaatatctat aaaataaatt atttgaatat
    35701 tcataaatca aaatttattt gtatgtactt cttcaagagt caatatgaat tttttcaaaa
    35761 aattaatttt gatgataatc attaagggct atatttttcc taaagttgtg attatcatta
    35821 ttaatttact aaaatacttt aatgaaaaat agctaacttt aattgtagga tattcttaat
    35881 atatttgaaa taaataaaaa tattgaagga tataatattc aattggtata taaataataa
    35941 atgatagtta agatttacct tagaggctaa ttaggtgttc agtcaaaatt aatgccttta
    36001 gaatacaaat gtattctaaa ggcattttta ctatgtattt tcatttatat tactaaacat
    36061 tatctatttt tatatacatt tctatgtatt ttattttcat caaatcggtc tataaattca
    36121 tttttttctt cactatatcc aatagatata agtgcataag gaactaattt tactggtaag
    36181 tctaaaattt gtttaacttt atttactact ttatttttag gaaatactcc tgcccaacaa
    36241 gcagctagac cttgattagt aacttctaat aataaatttt gtgtgcaagc acctaaatct
    36301 tgtatccatt taccaggttt taagaattta tctctatttc caagcactac tatacataat
    36361 ggtgcaaatt ctatatgctt tgctctatgc tgtgactttg agagctcttt cagcagttcc
    36421 ttatcatcta ctataataaa ttcccaaggt tgagcatttt ttgatgaagg tgcttgcata
    36481 gctgatttta gtatttcagt tattttatct tcttctatat ttttatctat atatgttctt
    36541 attgaacgtc tattttttat aacttctaac atacagattc tcctttaaat aaaattattt
    36601 ctatttatat aatatcatta ctttaagttt tagctattac ttatataaag aataaacttt
    36661 gtgttggttt tatctaagta aaagtaacat tcagaactaa aaaacaagac tataaatcca
    36721 agatattaat taaaaaagaa gtaataaaaa ataatataca agatttaaaa aagagtattg
    36781 agcttaaaat ttaattagaa aaccaatttt gagataatct gttttttatt ttaacaaaaa
    36841 aacataaata aagttcgata cgtagcgaat gaaaaaaata caagaaatat tttttttaaa
    36901 aaatttcaaa aaagagttga taatatatgt atattaatat aaaatttaag gtgtataatt
    36961 cgtataataa tacgaatgat ttgtagtgta aaataactta taaacggaga ggggaagtct
    37021 aatgaaagta gcagtagtta tgggttcaaa atcagattat ccaaaattag aagaagggat
    37081 aaaactgctt gaaaaatatg gaatagaggt tgtagcaaga gcattatcag ctcatagaac
    37141 accagaacaa ctttcaatat tcctaaaaga aatagaagat gatactgatg taataatagc
    37201 agcagcagga aaagcagcac acttaccagg tgtaatagct tcacaaacat taattcctgt
    37261 aataggatta cctattaaat catcaactat ggatggacta gactcacttt tatcaatagt
    37321 tcaaatgcca aaaggaatcc cagttgcaac agttacaata gacttaggtt taaacgcagc
    37381 tttattggca ttacaaataa tgactttaaa atacccaaaa ttaaaagagg atttgaaatc
    37441 atatagagaa gagatggcac aaaaggtgtt agaagacgac aagaatttaa gggggtagca
    37501 gaaatgttat tatatgaagg aaaagcaaaa caagtatatt ctacagataa tgaaaatgaa
    37561 tatgttgttt attataaaga tgatgcaaca gcatttaatg gagaaaaaaa agcagaaata
    37621 tcttcaaaag gtatactaaa taataagata tcaacaataa tctttgaaat gttaaaagaa
    37681 aataatataa atactcactt tataaaaagt ttatcagata gagaaatgtt agttaaaaaa
    37741 gtagagattt taccattaga agtaatagta agaaatattg ctgctggttc tatctgtaaa
    37801 agagttggtc ttgaagaagg tgtagttttc gatgagccaa tttttgaaat aagttacaaa
    37861 aatgatgctt atggtgaccc aatgctaaat gatgattatg cagtggcaat gaagttagca
    37921 actagagaag agttaaaatt cttaagagaa gaaactttaa aaataaacga gttactaaaa
    37981 gcatttttct taaaattaaa cctaaagtta gttgacttta aaatagaatt tggtaaggat
    38041 tctgaaggaa atataatatt ggcagatgaa gtatcaccag acacttgtag actttgggat
    38101 gtaaatacaa atgaaaagtt agataaggac agatttagaa aagaccttgg agaccttgta
    38161 gaaggttata cggaagtact ttcaagaatg aataataaat aggaggcaaa tgtatgtgtg
    38221 gagttttagg gatttactct aataaagatg taactaaaga actatattat tctttatatt
    38281 ctatgcaaca cagggggcaa gaaagctgtg gtttagctct tttagatgat ggagaaatta
    38341 agtacaaaaa agatatggga ttagttggag atgtttttaa ggaaaatgaa ttatctaagt
    38401 taaaaggaaa tatagggatt ggacatgtta gatattcaac agctggagga agtcatgttt
    38461 ctaactgtca acctttagtt ggaagttgta gaaaaagaca attagccata gctcataatg
    38521 gaaatctagt taatgcaaat tatttaaaag atatgctaga agaagatgga tatatgttcc
    38581 aaactaattc agatacagaa gtgattttat atatacttgc tagatattac aaaggggata
    38641 tagtagaaag tttaaaagtg acaatggatt atataaaagg tgcatatgct cttgtaataa
    38701 tgagtcaaga agaattggtt gcagttagag acccacatgg attcagacct cttgtacttg
    38761 gtaaaaaggg tgatgaatat atatttgcat cagaaaattg tgcaatagat atacttggcg
    38821 gagaagttat aagagatgta gagccaggag agataatagt agtcaaagat ggagaactaa
    38881 aatcatattt ttattcagaa aattataaac cagttaagaa aagttgtata tttgaacaca
    38941 tatactttgc aagaaatgat gcaactatag acaatgtaaa tgcatatgaa tttagaatta
    39001 aatgtggtga gagattagct cagaatgaaa ctgtaaaggc agatatggta gttccagttc
    39061 cagattcagg ttggccagga gctattggtt atgcaaatgc tagtggatta aaaataagtg
    39121 aaggcttagt taaaaataga tatgtaggaa gaacatttat aaaacctaca caagaagaaa
    39181 gagaaatagc agtaaaaata aaattaaatc ctctttcaac aataataaaa ggaaaatcta
    39241 taattttagt agacgactca attgtaagag gaactacatc taaacaatta gtaaaatctt
    39301 taagagaagc aggagcaaaa gaaattcatc ttagaataac ttctcctcca gtagcttact
    39361 cttgttacta tggaatagat actccaaatc gctctaaatt aattgcatca agtaacaacg
    39421 tagaagaaat gagagaatat attggatgtg atagtttaaa attcctagat atagaaggaa
    39481 tgttagatgc aacagaacat aagtctactt tctgtaaagc ttgttttgat ggagaatacc
    39541 cagttaagaa aattgataag gaggaactac tttcatgtta acatataaag aatcaggtgt
    39601 agatatagat gaaggaaata gagcagtaga ccttataaaa ggtaaaataa aaggaactta
    39661 tgatggaaat gtagttggag atttaggtaa cttcagtgga ttatacagtt taaaagattt
    39721 tgtaggtatg aaagaaccag ttttgcttgc atctacagat ggtgttggga ctaagttaaa
    39781 aatagctcag atgatggaca agcatgatac tgttggaata gatttagttg caatgtgcgt
    39841 gaatgattta atttgtcaag gagcaaaacc attatttttc cttgactata tagctcttgg
    39901 gaaattagtg cctgagcata tcgaaaaaat agttggtgga attgcagatg gttgtaaaat
    39961 gtctggttgt gcactaattg gtggagaaac tgctgaaatg cctggaatgt atggagaaga
    40021 tgactatgat ttagcaggat tttctgtagg aatagcagac aaagaaaaga ttgtttctgg
    40081 aaacaatgtt aaatcaggag atgtattggt tggtatatct tcaagtggag tacacagcaa
    40141 tggattttct tttataagaa agatattttt agaaacttac aattataaaa tggaacaata
    40201 tgttgaagaa ctaggaatga cagttggaga agcactttta acaccaacta aaatatatgt
    40261 taaattagct ttagatgtat tagctaaaca tgacataaaa gctatagctc acataactgg
    40321 tggagggctt atagaaaata taacaagagt tatacctaaa gggttaggac tagatatcaa
    40381 taaaaaatct tgggagaagc ctcctatatt taaaatgata gaaggtttta atgctgttga
    40441 tgagagagaa cttcataaga gttttaacat gggtattggg ctagttttaa tagtagataa
    40501 agaaaatgct gatgatgttg taaactttat aaataataga gaaaatgaca atgctgctta
    40561 tgtagataaa aaatacagtg aattattaga agataaagcg tatataattg gtgaagtagt
    40621 agatagtcat gaaggtgtag aattatgcta aatataggag ttttaatatc tggtggagga
    40681 acaaatttac aagcagttat agatggcact gagtctggag aaataaaagg tcaggttaaa
    40741 gtagttatct caagtaagca aggtgcttat gggctagaga gagctaaaaa tcataacata
    40801 aaagcaattt gtgaaactga tgaagacaag ataatagaga ttcttaagga aaataaaata
    40861 gatttagtag ttttggctgg ttatttaaaa ataatcagcc caaagctggt taatgagttt
    40921 agaaacaaaa tgataaacat acatccatca ttaattcctt cattttgtgg agctgggttc
    40981 tatggagaaa aagttcatca aggtgttata gattacggag ctaaagtaac tggtgctaca
    41041 gttcattttg ttgatgaagg tgctgatact ggaccaatca taatgcaaga tgtagttaag
    41101 gtaaatcaag atgatgatgc aaagacactt gcaaaaagag tgttagaagt tgaacatagg
    41161 attttaaaag aaagtataag tcttttttgt gaaaataaat taaaacttca aggtaggaga
    41221 gtgtttataa atgagtaaga gagctttaat aagtgtaact gacaagactg gtgtagtgga
    41281 atttgctaag gagttaaata agttagatta tgaaattata tctacaggaa atacatttaa
    41341 aacattaaaa gaaaatggtg ttaatgtaat gcaagttgaa gatgttacaa attttccaga
    41401 aatattagat gggagagtaa aaacattaaa tccatatata catggtggta tactttataa
    41461 aagagataaa gaatctcatg tagaaactgt gaatgagcat aaaatacatt caatagattt
    41521 agtagctgta aatctatatg attttgaagg aactttaaaa gctggaaaaa gtcatgatga
    41581 aataattgaa aatatagata ttggtggacc atctatgata cgttctgctg ccaaaaacta
    41641 taaagatgta atagtagtag tagatataaa agattatgat tctataatag aaaaattaaa
    41701 aacagataca atgacattag aagacagaaa aaaattatca tataaagctt tctcaactac
    41761 aggaagatat gatgcgttaa tatctagtta ttttgctgga gaagttggag atacatatcc
    41821 tgacatatta aatttaactt tccaaaaaga acaaacttta agatatggag aaaatccaca
    41881 tcaaaatggt ttcttatatt cacaatcaaa tgcaaaaaat ccaatcttaa attatgagca
    41941 attaggcggt aaagaacttt cttttaacaa tttaaatgat ttacatggat gtttagaagt
    42001 aatgagagaa tttaaagata gtgaagaagt agtttcagta gctataaaac atgctaattc
    42061 atgtggggta ggtctaggaa aagatgcttt tgaagcatat acaaaatgtt atgaagcgga
    42121 taaggtttca atatttggag gaatagtagg aataacttct actatagata aagctacagc
    42181 agagaaatta aatgaaatat tcttagagat agtagtagca tatgattttg agccagaagc
    42241 tttagaggta cttaaacaaa agaaaaattt aagaatatta aaattagcta agatagaaga
    42301 tagtttacaa ccatatgaaa tgaaatattt agatggaaaa ttattaatac aagatagaaa
    42361 taatatatta gcagaaaaaa gtgaaaatgt aacaaaagca aaaccaacag atgcacaact
    42421 taaggacatg gaatttggta tgagagtagt aaaaaatatg aaatcaaatg caattgcgat
    42481 agtaaaaaat ggtcaaactt tagctttagg ttgtggacaa acatcaagaa tttgggcatt
    42541 aaaaaatgct cttgaaaata acaaagataa agattttaca ggagctgttt tagcgtcaga
    42601 tgcatttttc ccatttgatg attgtgtaac attagctcat gaatatggaa tatcagcagt
    42661 agttcaacca ggtggctcta taaaggataa agattcaata gaagcttgtg ataagtatga
    42721 tatggttatg gtatttacag gaataagaca cttcaaacac taaggaggat attatgaaga
    42781 tattggttgt aggcggtggc ggaagagagc atgctatatg ctggaaacta agcaaagaaa
    42841 aaaatgtaga aaaagtttat tgcgcgccag gtaatgcagg aattacaaat gtagcagaat
    42901 gtgtaaatat aggtgatact aatatagaag aacttttaaa atttgccaaa gaaaatgaaa
    42961 taggtctgac aatagtagga ccagaagtac cactagttat gggaatagta gatgagtttg
    43021 aaaaagaagg attaagagta tttggaccta ataaaaaatg tgctcaactt gaaggaagta
    43081 aagctttttc aaaagaattt atgataaaac ataacatacc tactgcaaaa tataaagaat
    43141 atacaaactt agaagaagct atatcagaaa tagatagttt tggatatcca gtagtaataa
    43201 aagcggacgg attagctgct ggaaaaggtg ttgtaatacc tgaaaataga gaagatgcta
    43261 tagcaacttt aaaagaaatg atgtcagata agaagtttgg agctgctgga gataaaatag
    43321 taattgaaga gtttttaaaa ggaatagaaa cttcaattct agcatttgta gacaatgata
    43381 caatagttcc tatggcaagt gcaaaagacc ataaaaaagt aaacaactat gagcaaggtc
    43441 caaacactgg gggaatggga actttttcac ctagtgaaat atatacagaa gaattagcaa
    43501 ataaagtaaa agagacagta ttagaaaaaa ctttagaagg cttcaaaaaa gatggactta
    43561 attttaaagg tattttattt gttggtctta tgataactga agatggagaa aaagtacttg
    43621 aatataatgt aagatttgga gaccctgaga cacaatctgt tttatttaga ttagaaacag
    43681 atttacatga gataatggaa actatactag ataataagtt aaaagatata gaaataaact
    43741 atagtgatga tgaagctgta tgtgttatgc ttacatcagg agggtatcca gacagttatg
    43801 aaaaagggaa aattataact ggtcttgaaa acttggatga tgatatagta gtattccata
    43861 gtggcactaa gatgcttgat ggtaatttag ttaccaatgg aggaagagta attggtataa
    43921 ctgcaaagtc aactacagtt aaggatgcag cagaaaaagt ttatgaaaac ataaagaaga
    43981 taaactttga aggaatgcac tatagaacag acataggaag ataatttttc taaaatggag
    44041 gataaggtat gttgaatact gaaaataaag actcaatggt tagaagagta ttagttgaaa
    44101 aaagagaagg ttttgacctt gaggctaaag ctttaaaaaa agacttagta gaaagtttac
    44161 acatagataa tatagaaaat ttaagaatat taaatagata tgatgtagaa ggaatatcag
    44221 aagaagttta tgaaaatgca gcaaagacaa tattctctga accaaattta gatgtagtat
    44281 attatgaaga aataccaaaa ttaaatgatg aaagagtatt tgcaatagaa tttcttcctg
    44341 gtcaatacga ccaaagaggt gattgggcag ctcaatgtgt acaaattgta aatcaaggga
    44401 taagacctgc tattaataca gcaaaagttt atatattatc aggcaaaata actgatgaag
    44461 agttttctaa aataaaagat tactgtataa atccagtaga tagtagagaa gcatctttag
    44521 aaaaaccaga aacattaaaa atggaaactg aaataccaac aacagttgaa gtattagatg
    44581 gatttataga tttagatgaa aatggattaa gaacatttgt aagtgaaaaa ggattagcaa
    44641 tgactttagg agacttacaa cacgttcaaa aatactttaa agatacagaa aagagaaatc
    44701 caactataac agaaataaaa gtattagata cttattggtc tgatcattgt agacatacta
    44761 cttttatgac tgaaatagaa aatgtaaaga tagaagatgg taagtttaat gatatagtaa
    44821 aagaagcgta tcaaatgtat cttaactcaa gagacaatgt atatgtgaac agacataaag
    44881 atatatgttt aatggatata gccactgttg cagtaaaaga attaaagaag aatgggaaat
    44941 taaatgacct agatgaaagt gaagaaataa atgcatgtag tataaacgta gatgttgaag
    45001 ttgatggtaa gatggaaaaa tatctagtaa tgtttaaaaa tgaaactcac aaccatccaa
    45061 ctgaaataga accatttggt ggagcagcta cttgtttagg tggagctata agagacccat
    45121 tatcaggaag aagttatgtt tatcaagcaa tgcgtgttac aggaagtgct gacccaagaa
    45181 cgactttaga agatacttta cctggaaaac ttatgcaaag aaaaataact acagaagctg
    45241 ctcatggata tagttcatat ggtaaccaaa taggtcttac aactggacaa gttgcagaag
    45301 tatatgatga aaactttgtt gcaaagagaa tggaaatagg tgcagttata gcagcagtac
    45361 caaaagagaa tgtagtaaga gaaagacctg aagcaggaga tgtaatagtt cttcttggag
    45421 gaaaaacagg aagagatggt tgtggagggg caactggttc atctaaagaa catagtgaag
    45481 agtcaatctt aacttgtagt gcagaagttc aaaaaggaga tgctcctaac gaaagaaaaa
    45541 tacaaagatt ctttagaaat aaagaagttg ctcaaatgat aaaaagatgt aatgattttg
    45601 gagcaggtgg agtttgtgtt gctataggag agattgctga tagtttagat ataaacttag
    45661 acttagtacc taaaaaatac gatggacttg atggaactga gcttgcaatt tcagagtcac
    45721 aagagcgtat ggcagtagct ataaaaaaag aaaataaaga taaatttata caattagcag
    45781 tagaagaaaa cttagaagct acacatgtag ctacagtaac agatacagga tatttaagaa
    45841 tgttctggaa tggaaaagca attgtagata taaatagaga gttcttagat acaaacggag
    45901 taaaacaaac tactgatgtc catgttacta aagtagatga agaaaataca ttctttagct
    45961 ctaatgaaat agtaaaagat gttaagtgtg caagtatgaa agacaagttt acaaaagtat
    46021 tatctgacct taatgtttgt tctcaaaaag gattagtaga gatgtttgat aatacaattg
    46081 gtggtaatac agtacttatg ccatttggag gaaagtatca agcgactcca acacaaggta
    46141 tggttgctaa gataccagta cttggaggag aaactaatac ttctacaata atgacttatg
    46201 gatataatcc aaaggtagga aaatggagtc cattccatgg tgccttgtac gcagtagtag
    46261 aatctgtgtg taaattggtt gctattggag gaaattatag tacaacaaga cttacattcc
    46321 aagagtattt tgaaaaactt ggtaacaatc cagaaaaatg gggtaaacca ttctcagcac
    46381 ttttaggagc attttatgca caaagtaaat ttgagatacc tgctataggt ggaaaagaca
    46441 gtatgtctgg tacatttaaa gatatcgaag ttccaccaac attagtttca tttgctgttg
    46501 atacagtaga tgcgaagaaa gtagtatctc cagaatttaa aaaagcagac tcaaaagtag
    46561 taatgttatg tgtaaacaag gcagaaaatg atgttgtaga ttttgaggaa ttaaagagaa
    46621 acttagacaa agtaagagaa cttatacatg gaaataaagt tttatcaaca tatgcattag
    46681 gatttgcagg tgtaggtgaa gcaataagta aaatggcttt tggtaataaa ataggattta
    46741 agtttagtga agaagctgaa aaaacattta cagatgataa attatttgag gctagttatg
    46801 gaaatatagt acttgaatta gcaaatgatg atttatgtat gttagaagga tacaattatg
    46861 ttgtgcttgg aagtacaact aaagaagcta gtatatttat caaaggtgaa gaacttgcat
    46921 tagatgaatt atataaagca cactgtagta ctttagagcc tatattccca actaaaacag
    46981 aagaagtaaa atcaaagata gagactataa gttatatatc acaaggagaa gctaaaaaat
    47041 catcattaag tatagctact ccaagagtat ttataccagc atttcctgga acaaactgtg
    47101 aatatgactc tgcaagagca tttgaaagag taggagctaa tgcaaacata agagtattca
    47161 agaatttaac ttataaagac atagaagatt ctatagatac aatagtaaac gaaataaaat
    47221 cttctcaaat aataatgtta ccaggtggat ttagtgctgg tgatgagcca gatggttctg
    47281 gtaaatttat agctactgta tttagaaatc ctagagtgca agaagctata aatgaatttt
    47341 taactcaaaa agatggacta atgttaggta tctgtaatgg attccaagta ttaattaaat
    47401 taggattagt tccatatgga gaaataagag ttccaagtga aagtgctcca acacttactt
    47461 acaataatat tggtcgtcat caagctaaga ttgctagaac tagaatttca tctaataaat
    47521 ctccttggtt agctcaaact aatgttggag atattcataa tatagcaatt tctcatggag
    47581 aaggtaaatt tgttgctagt gaagatgtta tgagagaatt aattgctaat ggacaagtag
    47641 caactcaata tgttgacttt aataatgaag ctacttatga tattgagttt aatccaaatg
    47701 gttcattcta tgctgttgaa ggtataacta gtgctgatgg tagagtgttt ggtaaaatgg
    47761 gacattctga gagaattggt gaggaagtat ttaagaatat tataggtgaa aaagaccaaa
    47821 agatatttga atctggagtt aaatatttta gataaagctt tgatgtttaa ttgatagcta
    47881 gaatatataa tttagatatt tagatttaaa gagatgtaat aatatgatga taatcattta
    47941 atattacatc tcttttttat ttatctaaga aataataagt tcaacctaag ttaaaataca
    48001 agctgtgtta taattaatta ctaagtaaaa ttaaatattt attggagggc aacataatta
    48061 atgaaaatat taataacagg gtctaatggt caattaggaa aagaattggt tagccaatta
    48121 gaagcaatca atcaaagtat aaatcaacca aaatacgtta tattagctac aacaagaaat
    48181 gatttggata tatccaatca gactaatata gataatttta ttttgcataa taaacctgat
    48241 gtaattgtaa actgtgcagc atatacaaag gttgatgcat gtgaagataa tatcgaaatt
    48301 gcgtataaaa taaatgcctt aggagtgaga aatttagcaa tagcatcaga aaaagttaat
    48361 gctaagcttg ttcacatatc aactgactat gtattcaatg gtttttctaa atatccatat
    48421 agggaagaca ataaaactga acccaattct gtgtatggaa aaagtaaact tatgggagaa
    48481 aagtttgttg aacaattctc acataagtac tttatattga gaacagcatg gttatatggt
    48541 aatgggaata attttgtaaa gactatgata aaactttctt tagaaaataa agaggttaat
    48601 gtggttaatg accagtttgg aaccccaaca agtacagtag acttagcaaa aattataata
    48661 agaatcatgg aaacagaaca ttatggagtt tatcatgcta catgtgaagg tgagtgtagt
    48721 tggtatgatt ttgccaaaaa gatatttgaa ttaaaaaata tagatataaa agtaaatcca
    48781 attaagagta acgaatttaa atgtaaggct caaagaccac tatattctgt attggataat
    48841 tttatgttga agttaattgg tttaaattct tttagaaagt gggaagaatc cattgaagaa
    48901 tatttagaaa gatatgatat aaatgaaaag taaatgtatt atttttagaa aagaaaataa
    48961 gatattataa aaaatataaa tatttctttt atttatccga taatcataat atgacaaaaa
    49021 caaatttaag gatggaaaaa gatgaaagga attattttag caggagggtc tggtacaaga
    49081 ctatatccta taacaaaatg tatatcaaaa caaacacttc caatatacga taaaccaatg
    49141 atatattacc ctatgtcggt tcttatgttg gcagggatac aagatatatt aataatatct
    49201 acaccaagag atataaaagc atttgaagaa ttatttggag atggaagttc actagggctt
    49261 aaaataacat atgcaattca ggagaaagca aatggaattg cagaagcgtt tataatagga
    49321 gaagaattta taggaaatga cagagtgtct cttgtattgg gggataatat attttatgga
    49381 tatggattta gtgaaaggtt ggagaatgca gttaaaagaa aaggagcaac tatatttggg
    49441 tatcatgtaa gtaatccaaa ttcgtttgga gttgtagaat ttgataagga ttttaatgta
    49501 ttatcaatag aagaaaaacc tacagttcct aaatctaatt atgctatacc aggattatat
    49561 ttttatgata atgatgttgt agagatagca aagctagttg aaccatcaga tagaggtgaa
    49621 ttagaaataa cctctataaa cagtgaatat ctaaaacgag gaaaactaaa agtggaactt
    49681 ctaggaagag gtatggcttg gcttgatact ggtacacata aaggtctatt ggatgcttct
    49741 aactatgttg aagtggttca aacaagacag ggactgtata tagcatgttt agaagagatt
    49801 gcatatacaa aaggatatat aagtagagaa caattaatga aactagccaa gccactgata
    49861 aaaactgaat atggtcaata tctattaaaa ttggctagtg aagatatgca atatggtaga
    49921 taaaatttat tataaatttt attaagggag tttgttataa tgggaaaaat caatgttata
    49981 aagactaaaa tagaaggatt atatatagtc gaaccaaatg tattttgtga tgaaagagga
    50041 tattttatgg aaagctataa taaaaaggat tttatagagc agggtcttga tatgtgtttt
    50101 attcaagata atgagtctag ctcaagaaaa ggtgttttaa gaggaatgca ttttcaggtt
    50161 agatatcctc aaggaaaatt agtcagagtt acagagggag aagttttcga tgttgcagtt
    50221 gatttaagaa aagattcaca gacatttggg aaatgggaag gtgtaatatt gtctgataag
    50281 aataaaaaac aattttatat tccagaaggg tttgcacatg gatttttagt tttatcagaa
    50341 aaagcagtat ttaattacaa atgtacaaac tattattatc cagaatttga gcatgggttt
    50401 atatggaatg acaagcaaat atctattgag tggcctttaa aaggaattga agaaatattt
    50461 ttatcagaaa aggacaaaaa acaaaaaagt tttaaagata tttgtataag acgttaataa
    50521 aaatttatta gaattaacat ttaggagaaa aaatatgaaa aaaatactga taactggagg
    50581 agcaggattt attgggagta actttgtaca ttatatgctt aacaagtatg tagattattt
    50641 tattgttaac ctagacttgt tgacttatgc tggtaattta gagacattaa tagaaataca
    50701 agacagttcc aattataaat ttgttaaagg agatatttca aatagggaac agatgtataa
    50761 gctatttgaa gaagaaaggt ttgatatagt tgttaacttt gccgctgaat ctcatgtaga
    50821 taggtcagtt gagaatcctg atttatttat taaaacaaac attataggaa cacaagtact
    50881 tttagatgca tctataaaat atggagttaa aaggtatcat caaatatcta cagacgaagt
    50941 gtatggtgat ttaccaattg atagaaagga tttattcttt acagaacaat ctcctatcaa
    51001 tccaagttct ccatattcag cttctaaagc atcagcagat ttgttggtaa gttcttatta
    51061 tagaacatat ggattgttaa caactatatc taggtgttcc aacaattatg ggccatatca
    51121 ttttcctgaa aaattgatac ctcttatgat tttaaatgct ttggaaaaca aacaattgcc
    51181 agtatatggg aatggggaaa atgtaagaga ttggttacat gtatatgacc attgtactgc
    51241 tatagactta ataatacata aagggaatat aggggagatt tacaatatag gaggtcataa
    51301 tgaaagaagt aatttagaag tagtaaaaat gatattaaat cttttagata aatctgaaga
    51361 gttaatatcc tatgtaaatg atagacctgg tcatgattta agatatgcta ttgatgcaac
    51421 taaaatagag aatgagttag gttggaaggc aaaatatgac tttgatttgg gtataaaaga
    51481 aactgttaag tggtatatag aaaatgaatc ttggtggaaa gctgttttat cagtgtaact
    51541 ttgaaattta taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt
    51601 aatatattag cattgactca attatctggt agtaatacta caaatcaatg taactgtaca
    51661 tataatagtt catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa
    51721 aataatcaac agacttctaa tggctacaat ttatcgtcta attctgagaa tttatttgac
    51781 gaactcggca gtgtagtaaa taatacagct agtagattta tagatgttga tacaaaagat
    51841 aaaagtgtaa aatcaagaat tgaaaatgct atagagcaag catcaaaaaa atataatgtg
    51901 gattcgaatc ttataaaagc aataattaaa gttgaatcgg attttaatcc aaatactgtt
    51961 tcttctgctg gtgctaaggg tcttatgcaa cttatgccag aaaattgcag agacttgggg
    52021 gttacagacc cttttaatat agagcagaat atagatgctg gaactagaca tattaaggaa
    52081 tacattgata tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct
    52141 ggaagaatga aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa
    52201 acacaaaatt atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggga
    52261 taaatataga taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag
    52321 agagtaataa attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata
    52381 taaaacgaaa tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact
    52441 atggcgacat tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata
    52501 atatgtattc acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa
    52561 agattgttga tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa
    52621 acatagatat tgtttctact gttgatatga tacaaggctt gttgatagat atgtatagat
    52681 aaggttatac aaggagaaaa gttttatgaa tagtaaaaaa gaggatatat atgaattgaa
    52741 ttttgggcaa ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa
    52801 tgcaaaagta aatgtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt
    52861 agatttaaaa gaaggctcta taataaaact aagtaagaat gtggatgaaa aattagatat
    52921 atatgcaaat gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc
    52981 tgtaagatta tctaaaatag aagattcaga tgtaaattag tattttaaat agtatccctt
    53041 taggctatga tgtatgagtt tagctaaggg gatattttta taaaaaattt aaaatatgcg
    53101 tgaattatat aaatgttttt attttatgag cgataattaa attataagta tgtaattttg
    53161 gaggtgaaac tatggatatt aaaaatgtaa gttcaaatat tgtagtgaat aattatttaa
    53221 agaatacatc tggtgtaaaa aaagtggaag aagttaaaaa aattaataaa acagacaaga
    53281 tagagctatc taaatttgca aaggaaatgg caagtgtgtc aaatatgagt gatgagaata
    53341 ttaggaataa aaaagtcgat gaaataaaaa atgcattaga aaatggaact tacaaaataa
    53401 aaccaaaaga aattgcgaaa aaaatgatag aggatatgcg aggataaagt catgattaca
    53461 gacttaaaag ttataatata tgaagaaaaa aatataatta gtaagatgat ggaattatta
    53521 aaagaacaat ttgattatat agttgaaaaa gacttagaaa atttaaatat gttaaatcct
    53581 aaactagaag atattagcag ggagcttgca tccatagaaa taaagagaag acaacttttt
    53641 ggtgatgata tttcaatgag tgaagttgtt gaaaatagta atgatagata tttaaaagaa
    53701 atatatacag acttgaaaca cattcttgct ttagctaaaa atcaacaaga atcaaatgat
    53761 tctctaataa agaaagaact tatttttacg aaaaaaatga ttaatttcat aaaaccagct
    53821 gataagcaaa ctgcaactta taattcttat ggaaatatta gaaaataaat attaggagga
    53881 gaaatatggc aggattattt tccattttaa atacagctaa ttcaggaatg aatgcgcacc
    53941 aaaaagcaat acagacaatt tctcataata taagtaatct agatacagac ggttattcta
    54001 ggcaaaggac tgagtttgct acaaatagtc caatgtatat gcctagtttg agtaattcaa
    54061 ttgcaagagg acaattaggg actggagtac acgtaacgga tgtaacaaga gctagaaata
    54121 gtttttatga ttaccaattt agagcagaag ctcataagta tggtaataca gtttcaaagt
    54181 atgactatta taatactata gaaactataa taaatgaacc atcagactat ggaatatctg
    54241 caggtataga cgactttttt aaaggatggg aatcattagc aaaggatata aatagtgata
    54301 gtaaaaaaag tctagtggta gaaaatgcat caaatctagc aactttaata agtgaaagtt
    54361 ataagaagct taataagtta aagggagata tagacgataa tataaactct gaaataaaac
    54421 agataaatga catgttaaaa agtcttgaag atttgaataa atctattgat gttatatctg
    54481 gttctggctc tactccaaat gatttacttg atgaaagaga tagaatatta gatgacttaa
    54541 gctttaaatt agaccttgaa aatagcgatg ttaaaaatat gctttcagat ggtaaattag
    54601 aacttaatga attaaaaaat gcagatggaa cttggaaaac aggtgtatca ggaaccttac
    54661 aaggcttgtt tgaaatgcat ggtaagattg atacatataa aagcgattta aaagatgtat
    54721 ctgatgggtt agctaagcag ataaatgatg tttataatac atctgctgga ataacagtaa
    54781 cagatttttt tataacttca aatgtagatg gtgaagatat cataaaggta aatccagcta
    54841 taaagagtaa cccaaatgaa ttaaaactta ctgctgatga ggcttcaaag attgctaagt
    54901 taaaggatga aaaaatagat attggggtgg ctggtggaaa agtaagtaca ataagtgacc
    54961 actataaagc ctttgcagaa tcagtaggtt tagattctca aaaagtgaat caagatgaag
    55021 tgaatcaacg taaaattata aataatattg ataactcacg aatgagtgtt tcaggcgtat
    55081 ctttggatga agaaatgaca gagcttataa aaatacaacg ctcctatcaa gctagtgcaa
    55141 aagttatgtc aacagctgtg caactgttgg atgttgtaat aaatggaata atttaataga
    55201 agggggaatt tataatgaga gttagtacag gtatgatgtc tagcaactat ctaaactcac
    55261 tacaagataa tctacaaaga ttagataagg ttaatagaca agttaataca acgaaagaaa
    55321 taaacaagct atcagataat ccatataagg caataaaaat attgaactca aaaagtgaaa
    55381 taaaaactat ggagacttat gttgagaatt gtaaagatac agggaattgg ttagaaacta
    55441 ctgatactgc attagatcag cttggaaaat tattatcgga cataaagaaa actttggtgg
    55501 caagtggtga tggtgcatat tcagatgatg agctaaagac tcttagtaat gaaactaatg
    55561 aaaagatgaa agaaattgca aatgccttaa atgcaacaca tgaaggaaag tacatatttt
    55621 caggttcaaa tacaggtgta ccacctgttg aatgtataga taatgctgac ggttcagtga
    55681 gcttaaagtt taattctagt ttaaatctta ataaattaaa tgactctttg agtatggata
    55741 tagctcaagg aataagtgta gattataatg ttaaattgag tagccttgga tttgacccaa
    55801 caaagactga cccatttgaa gagttaaata atatatcaaa gaaattagca aatccagatg
    55861 ctgatgctat aaaagagctg acaacaactt gtttaggcga catggaacaa ttaatagaaa
    55921 atacagtaaa tgttaggtct atatatggga caaaagcaaa tacagtagag gctatgaagg
    55981 aaaaaaatga tgatagtttg atacagttaa aggatatttt gtcacaaaat gaagaaatag
    56041 attatggaga aaaactcgtt caattaaagg cagcagagtt aacttatcaa gcttcactac
    56101 aaacaggagg aaagttattt aatgtatcta tattagatta catatagtaa aataacgcag
    56161 atatgatgaa ggttacatta aaaaaaggta tattaggttt tgaaaattta aaagaatatg
    56221 agcttttaga tatagaagat aatgattctt taaaagaatt taattctact gaagaagaag
    56281 gtataggatt tgttgttgta tctccatttg agattatcaa tgaatatgaa atagttttaa
    56341 atcaggaaac aatagaaaaa ctagaagtga aatctccaaa ggatgttgta ctatttaata
    56401 taattactct tggtcagaca tttgaagaat caactgtgaa catgaaagct cctatagtga
    56461 taaatataaa aactaatttt ggaatgcaaa taattttaca ggatgataaa tattcaataa
    56521 ggcatccatt attgagaggt gataatggat gctagtaatt tcaagaaaaa aagatgaagc
    56581 agtacttata ggagaaagca tagaagtaaa aattgtaggg gtagatggaa acaatgtgaa
    56641 actagctata tcggcgccta acaatataag tatattaaga aaagaaatat acgaaaaagt
    56701 aaagagtgag aatataaaag ctacaaataa aaatataaaa attttaaagt cattaaaata
    56761 ataagcttgg aggaagtaat atatgtatgg agaaaatcct tataattctt ataaacaaaa
    56821 tgctgttttt atggcatcaa aggaacaatt gttacttatg cttgttgatg gagcagtaaa
    56881 atataccaaa atagccagag cagctatctt agataaaaat acacagaaag cgcatagaga
    56941 acttgtaaga gttcaggata tatttacaga actaatggtt acattagacc aaaatgcagg
    57001 acaatgggca aaggatatgt acaaagttta tgattttgta agatatgaac tttcaagagc
    57061 caatattaga aaagacatcc aggtaataga taatgtgttg cctgtaatag aacaaattaa
    57121 agacacatgg catgaagctg ataaaaaaag taaagaagaa agaagtaggt acaaataatg
    57181 gaattaaatt tagctgaatt agataagtta tcaaaagagg aattactatt gatgcttgta
    57241 gatggcacag taaaatatac caatatatcc aaagaagctc ttttaaataa cgactatttg
    57301 aaagcacata atgaacttat aagagttcaa aatatattta cagaactaat gactacatta
    57361 gaccaagatg caggtcaatg ggcaaaggat atgtataaag tttatgaatt tataaaatct
    57421 gaattagcaa tagctgatga aaataaagat attaaaataa ttgatgatat attgccaata
    57481 gtaaaacaaa ttagagatac atggcatgaa gtttacaatc aactaaaaat ataattagat
    57541 ggagggataa aggtatgtca agtataagtc ctataagagt tacaggtctt tcaggaaatt
    57601 ttgatatgga aggcataatc gaagctagta tgattagaga caaggaaaaa gttgataaag
    57661 caaaacaaga acaacaaatc gttaaatgga agcaagaaat atatagaaat gttatacaag
    57721 aatcaaaaga tctttatgat aaatatctaa gcgtaaattc tcctaatagt atagtaagtg
    57781 aaaaagcata ctcttctaca agaataacca gttctgatga aagtattata gtagcaaaag
    57841 gctcagctgg tgcagaaaaa ataaattatc aatttgcagt ttctcaaatg gctgaaccag
    57901 caaaatttac tattaaatta aattcaagtg aacctattgt tcgacagttc cctccaaatg
    57961 ccagtggagc tagttcttta actataggag atgtaaatat accaatatct gaacaagata
    58021 ctacaagtac tattgtaagt aagataaact ccctttgcgc agataatgat ataaaggctt
    58081 cttatagtga gatgacaggt gaattgatta tttcgagaaa acaaactggt tcgtcatcag
    58141 acattaattt aaaagtaatt ggaaatgaca atttagctca gcaaattgct aatgataatg
    58201 gtatcacatt tgcaaatgat gctagtggaa acaaagtggc aagtgtatat ggaaaaaatc
    58261 tagaagctga tgtaactgat gaacatggaa gagtaactca tataagtaaa gaacaaaatt
    58321 catttaatat agataatatt gactataatg taaattcaaa aggaactgca aagttgactt
    58381 ctgtcactga tactgaagaa gctgttaaaa atatgcaagc atttgtggat gattataata
    58441 aactgatgga caaggtctat ggtttagtta ctactaaaaa accaaaagat tatccgcctc
    58501 ttacagatgc ccaaaaagaa gatatgacaa ctgaagaaat agaaaaatgg gaaaagaaag
    58561 ctaaagaagg tatacttaga aatgatgatg agttaagagg ttttgttgaa gatattcagt
    58621 ctgcattttt tggagatgga aaaaatatta ttgcattaag aaaactaggt atcaatgaaa
    58681 gcgaaaatta caataaaaaa ggtcaaatat catttaatgc agatactttt tcaaaggctc
    58741 ttatagatga tagtgataag gtatacaaaa cactagcagg ttattcttcg aattatgatg
    58801 ataagggaat gtttgaaaag ctaaaagata ttgtatatga atattctgga agttcaactt
    58861 ctaaacttcc taaaaaagca ggtatagaaa aaactgcttc tgctagtgaa aatgtatatt
    58921 caaaacaaat tgcagagcaa gaaagaaata taagcaggtt agttgaaaaa atgaatgata
    58981 aagagaaaag actttatgct aaatattcag ccttagaatc tttgttgaat cagtattctt
    59041 cccaaatgaa ttatttctca caagcacagg gtaattaatt atggataaat taaaagaaaa
    59101 attaaattta tataaagata tatctttaca aattataaat ttgattgaaa aggaagaata
    59161 tataaagata agtagtaagt taggtgaaag acaagagatt ataaatagtg taagtgaaat
    59221 tgatagaaat gattttatac agctttataa tagaatggaa ttaattgaaa ttgatagtag
    59281 gataagagat attttacaag gacaattatt agaagtaaaa aaagaattac atgagtataa
    59341 gcttactaaa caagtgaaca caatgtatta taacttaaat agagaaaaag taaatatttt
    59401 taataaaaaa gtataaagtt atgtaccatc ttgtccgatt atataaatga agaattaata
    59461 aaaaagatta aaagtagaaa tgacaaggat gtcaactata ctaaggaggg taaaataatg
    59521 agagttaata caaatgtaag tgctttgata gcaaataacc aaatgggaag aaacgtaaat
    59581 gcacaaagta agtctatgga gaagttatct tctggtgtta gaattaagag agctgctgat
    59641 gatgctgctg ggcttgctat atctgagaaa atgagagctc aaataaaagg tttagaccaa
    59701 gcaggaagaa acgttcaaga tggtatatct gttgtacaaa cagcagaagg tgcattagaa
    59761 gaaactggta atatactaca aagaatgaga actttatcag tacaatcttc aaatgaaact
    59821 aatactgctg aagaaagaca aaagatagct gatgagttgt tacaattaaa ggatgaagtt
    59881 gaaagaatat caagctcaat tgagtttaat ggtaagaaat tacttgatgg aagttctaca
    59941 gaaataagat tacaagttgg agctaatttt ggaacaaatg ttgcaggaac aactaacaac
    60001 aacaatgaaa taaaagttgc tctagtaaat acttcaagta taatgagtaa agcaggtata
    60061 acaagttcta caatagcaag cttaaatgca gatggaactt ctggaacaga tgcagctaaa
    60121 caaatggtgt ctagcttaga tgtagctctt aaagaattaa atacatcaag agcaaaatta
    60181 ggggcacaac aaaatagact agaatcaact caaaacaact taaataatac tatagaaaat
    60241 gttacagcag ctgaatcaag aataagagat acagatgttg cttcagaaat ggttaactta
    60301 tctaaaatga atatattagt tcaagcatca cagtcaatgc ttgctcaagc taatcagcaa
    60361 ccacaaggag ttttacaatt attaggataa aataattttg tatattggat atagaagaaa
    60421 gggtaaggct ttgctttact cctttctttt tttacaaata tacttttgaa aggaaaatat
    60481 atatgttact tagtatagtt atgattgtta aaaatgaaga aaaaatactt gaaaaaactt
    60541 taaaatcatt aaccacatta agaagttcta tagagagtga acttataata gttgatacag
    60601 gttctacaga tgatactata aatatatcta ggaaatacac ggaaaatgtg tactttcata
    60661 attggaataa tgatttttct agtatgagaa atatatcaat atcctatgct aaaggtgaat
    60721 ggttacttat attagatgca gatgaaatac taattgattc ttcaactatt gtgaaatttt
    60781 ttaaagatgg tcttgataaa aaatttaatt cggcctcaat acaattaaaa aatctttact
    60841 catatgataa aaaattatat ggatattgct cagtgcttag actttttaaa aacatcgatt
    60901 ttagatacca tggtaaagta catgaacaac caatatacaa aaaccctata tttaataatg
    60961 ttgctgattt tgaacattat ggatatttgt ttgaagatga agaaataaga ttaaataaag
    61021 taaaaagaaa tgaaaaactg ttatttgaag aattaaaaga gaatgataat tctccatata
    61081 caaattatca attagggaaa aattttatta tattaggaaa atatcatgag gctttatact
    61141 atcttgagaa atcttatgaa ttatattcta aactagaatc ggcacctggt tacttgataa
    61201 caagtcttgt aaaaacatat ttatatttag gaaagcataa gaagtgtgaa aaattgtgtc
    61261 taaagtatat taagaaagac atgaataata ttgatatata ttattatatt gcacaagcac
    61321 aagtagattt agggaagtat gaaaatagta tagattcgta taagagatat atttatttgt
    61381 tagataatta tgaaataagt acacaagcaa atagtttatt ttcagacact gatactgtag
    61441 ggcttaggga tgaagctatt attacactga taaagattta ttataaactt gaaaaatatg
    61501 agtctgtaat ttcagagtac aataacattg aggatatgga aaagaagaag aatgtgtact
    61561 ttagcttatt tatgtcccta tacaaattga acagatttga agatataaaa aactattata
    61621 aagaagtttc aaattctaaa attgaaaaaa atagttttta taaaaatata gaagaagtta
    61681 taaataatat taaagatgat gaaaaagagc atgtatataa aatcctttgt gacattgaag
    61741 gtaattatgg cattttaaat cagataagga taaataaatg tgtatctata gataagtgta
    61801 aagaaatact caatgaagaa aaagaaataa tttatgcata tattataaaa gtagcttttg
    61861 aagataagat tgatttacta gatatttttt atgaaatgga atatgtatgg atagagaaat
    61921 acctaaaata tcttttagca ctagaaagaa gttttaattt agtactatat aagtacataa
    61981 ttagtcgtcc gaatacatca gatataaaaa agataagagt atataaagct atatctaaag
    62041 tagttttaga aagtataagt ttaagtgatg aaaaatataa ggagattttt tatctttata
    62101 taatgtattc ttataagtac ataagataca tatatagaaa tttaagcgat tttgaattaa
    62161 taaaatttgt ttgcaatgat ttagataagt ttactataga atttaagcat ctacttgaca
    62221 ttaaaaataa agtaaatgat gctaatgaaa aattagagta tatacatagt atgag
//
LOCUS       DAEPOG010000004        61811 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEPOG010000004 DAEPOG010000000
VERSION     DAEPOG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.004"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            89..367
                     /gene="spoVG"
                     /locus_tag="KR432_000188"
     CDS             89..367
                     /gene="spoVG"
                     /locus_tag="KR432_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBH0638750.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            577..1956
                     /gene="glmU"
                     /locus_tag="KR432_000189"
     CDS             577..1956
                     /gene="glmU"
                     /locus_tag="KR432_000189"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH0638751.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            2051..3001
                     /locus_tag="KR432_000190"
     CDS             2051..3001
                     /locus_tag="KR432_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBH0638752.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      3841..3926
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            4088..4609
                     /locus_tag="KR432_000191"
     CDS             4088..4609
                     /locus_tag="KR432_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0638753.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            4760..6436
                     /locus_tag="KR432_000192"
     CDS             4760..6436
                     /locus_tag="KR432_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0638754.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            6448..7656
                     /locus_tag="KR432_000193"
     CDS             6448..7656
                     /locus_tag="KR432_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0638755.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            7671..9383
                     /locus_tag="KR432_000194"
     CDS             7671..9383
                     /locus_tag="KR432_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBH0638756.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            9376..10284
                     /gene="pilO"
                     /locus_tag="KR432_000195"
     CDS             9376..10284
                     /gene="pilO"
                     /locus_tag="KR432_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBH0638757.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            10311..10877
                     /locus_tag="KR432_000196"
     CDS             10311..10877
                     /locus_tag="KR432_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0638758.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            10914..11441
                     /locus_tag="KR432_000197"
     CDS             10914..11441
                     /locus_tag="KR432_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0638759.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            11462..13000
                     /locus_tag="KR432_000198"
     CDS             11462..13000
                     /locus_tag="KR432_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638760.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            13100..14182
                     /locus_tag="KR432_000199"
     CDS             13100..14182
                     /locus_tag="KR432_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH0638761.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            14163..14945
                     /locus_tag="KR432_000200"
     CDS             14163..14945
                     /locus_tag="KR432_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0638762.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            15047..15697
                     /locus_tag="KR432_000201"
     CDS             15047..15697
                     /locus_tag="KR432_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0638763.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            15711..16271
                     /locus_tag="KR432_000202"
     CDS             15711..16271
                     /locus_tag="KR432_000202"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH0638764.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            16288..19674
                     /gene="mfd"
                     /locus_tag="KR432_000203"
     CDS             16288..19674
                     /gene="mfd"
                     /locus_tag="KR432_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH0638765.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            19705..20700
                     /locus_tag="KR432_000204"
     CDS             19705..20700
                     /locus_tag="KR432_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0638766.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            20824..21363
                     /gene="spoVT"
                     /locus_tag="KR432_000205"
     CDS             20824..21363
                     /gene="spoVT"
                     /locus_tag="KR432_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBH0638767.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            21483..23096
                     /locus_tag="KR432_000206"
     CDS             21483..23096
                     /locus_tag="KR432_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0638768.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            23203..24651
                     /gene="mazG"
                     /locus_tag="KR432_000207"
     CDS             23203..24651
                     /gene="mazG"
                     /locus_tag="KR432_000207"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH0638769.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            24740..25018
                     /locus_tag="KR432_000208"
     CDS             24740..25018
                     /locus_tag="KR432_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBH0638770.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            25090..25332
                     /locus_tag="KR432_000209"
     CDS             25090..25332
                     /locus_tag="KR432_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBH0638771.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            25394..25648
                     /locus_tag="KR432_000210"
     CDS             25394..25648
                     /locus_tag="KR432_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBH0638772.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            25649..26182
                     /locus_tag="KR432_000211"
     CDS             25649..26182
                     /locus_tag="KR432_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638773.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            26188..26472
                     /locus_tag="KR432_000212"
     CDS             26188..26472
                     /locus_tag="KR432_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBH0638774.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            26585..27505
                     /locus_tag="KR432_000213"
     CDS             26585..27505
                     /locus_tag="KR432_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBH0638775.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            27595..29967
                     /gene="spoIIE"
                     /locus_tag="KR432_000214"
     CDS             27595..29967
                     /gene="spoIIE"
                     /locus_tag="KR432_000214"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBH0638776.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            30318..32210
                     /gene="pepF"
                     /locus_tag="KR432_000215"
     CDS             30318..32210
                     /gene="pepF"
                     /locus_tag="KR432_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0638777.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            32324..33289
                     /gene="galU"
                     /locus_tag="KR432_000216"
     CDS             32324..33289
                     /gene="galU"
                     /locus_tag="KR432_000216"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0638778.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            33789..35393
                     /gene="rho"
                     /locus_tag="KR432_000217"
     CDS             33789..35393
                     /gene="rho"
                     /locus_tag="KR432_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH0638779.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            35546..35746
                     /gene="rpmE"
                     /locus_tag="KR432_000218"
     CDS             35546..35746
                     /gene="rpmE"
                     /locus_tag="KR432_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH0638780.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            35891..36784
                     /locus_tag="KR432_000219"
     CDS             35891..36784
                     /locus_tag="KR432_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBH0638781.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            36802..37650
                     /gene="prmC"
                     /locus_tag="KR432_000220"
     CDS             36802..37650
                     /gene="prmC"
                     /locus_tag="KR432_000220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH0638782.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            37694..38758
                     /gene="prfA"
                     /locus_tag="KR432_000221"
     CDS             37694..38758
                     /gene="prfA"
                     /locus_tag="KR432_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH0638783.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            38887..39567
                     /locus_tag="KR432_000222"
     CDS             38887..39567
                     /locus_tag="KR432_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBH0638784.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            39596..40636
                     /locus_tag="KR432_000223"
     CDS             39596..40636
                     /locus_tag="KR432_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH0638785.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            40653..41102
                     /locus_tag="KR432_000224"
     CDS             40653..41102
                     /locus_tag="KR432_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBH0638786.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            41119..41571
                     /gene="rpiB"
                     /locus_tag="KR432_000225"
     CDS             41119..41571
                     /gene="rpiB"
                     /locus_tag="KR432_000225"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0638787.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            41607..42236
                     /gene="upp"
                     /locus_tag="KR432_000226"
     CDS             41607..42236
                     /gene="upp"
                     /locus_tag="KR432_000226"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH0638788.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            42536..43351
                     /locus_tag="KR432_000227"
     CDS             42536..43351
                     /locus_tag="KR432_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH0638789.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            43577..44014
                     /locus_tag="KR432_000228"
     CDS             43577..44014
                     /locus_tag="KR432_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0638790.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            44199..44426
                     /locus_tag="KR432_000229"
     CDS             44199..44426
                     /locus_tag="KR432_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBH0638791.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            44431..44817
                     /locus_tag="KR432_000230"
     CDS             44431..44817
                     /locus_tag="KR432_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBH0638792.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            44820..45524
                     /gene="atpB"
                     /locus_tag="KR432_000231"
     CDS             44820..45524
                     /gene="atpB"
                     /locus_tag="KR432_000231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH0638793.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            45593..45853
                     /gene="atpE"
                     /locus_tag="KR432_000232"
     CDS             45593..45853
                     /gene="atpE"
                     /locus_tag="KR432_000232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBH0638794.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            45976..46473
                     /gene="atpF"
                     /locus_tag="KR432_000233"
     CDS             45976..46473
                     /gene="atpF"
                     /locus_tag="KR432_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH0638795.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            46470..47015
                     /locus_tag="KR432_000234"
     CDS             46470..47015
                     /locus_tag="KR432_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH0638796.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            47032..48534
                     /locus_tag="KR432_000235"
     CDS             47032..48534
                     /locus_tag="KR432_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH0638797.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            48562..49440
                     /gene="atpG"
                     /locus_tag="KR432_000236"
     CDS             48562..49440
                     /gene="atpG"
                     /locus_tag="KR432_000236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBH0638798.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            49453..50847
                     /gene="atpD"
                     /locus_tag="KR432_000237"
     CDS             49453..50847
                     /gene="atpD"
                     /locus_tag="KR432_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH0638799.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            50850..51110
                     /gene="atpC"
                     /locus_tag="KR432_000238"
     CDS             50850..51110
                     /gene="atpC"
                     /locus_tag="KR432_000238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBH0638800.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            51572..51952
                     /locus_tag="KR432_000239"
     CDS             51572..51952
                     /locus_tag="KR432_000239"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH0638801.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            51967..52428
                     /locus_tag="KR432_000240"
     CDS             51967..52428
                     /locus_tag="KR432_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0638802.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            52449..53039
                     /locus_tag="KR432_000241"
     CDS             52449..53039
                     /locus_tag="KR432_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638803.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            53054..54211
                     /gene="alr"
                     /locus_tag="KR432_000242"
     CDS             53054..54211
                     /gene="alr"
                     /locus_tag="KR432_000242"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0638804.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            54244..54531
                     /locus_tag="KR432_000243"
     CDS             54244..54531
                     /locus_tag="KR432_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBH0638805.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            54500..54883
                     /locus_tag="KR432_000244"
     CDS             54500..54883
                     /locus_tag="KR432_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH0638806.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            55054..55881
                     /locus_tag="KR432_000245"
     CDS             55054..55881
                     /locus_tag="KR432_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0638807.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            55874..56815
                     /locus_tag="KR432_000246"
     CDS             55874..56815
                     /locus_tag="KR432_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0638808.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            57238..58332
                     /locus_tag="KR432_000247"
     CDS             57238..58332
                     /locus_tag="KR432_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638809.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            58558..58974
                     /locus_tag="KR432_000248"
     CDS             58558..58974
                     /locus_tag="KR432_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638810.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(59156..59725)
                     /locus_tag="KR432_000249"
     CDS             complement(59156..59725)
                     /locus_tag="KR432_000249"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH0638811.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            59879..61036
                     /locus_tag="KR432_000250"
     CDS             59879..61036
                     /locus_tag="KR432_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBH0638812.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            61223..>61811
                     /locus_tag="KR432_000251"
     CDS             61223..>61811
                     /locus_tag="KR432_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0638813.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT        24850 a         6552 c        11546 g        18863 t
ORIGIN      
        1 ttaaaaaaca gtttatatta atttattcca gcgataataa ttttaatata aattaatttc
       61 tgtcaaaatc aaagggggat attttggtat gaagataact gacgtaagag taagaaaatt
      121 aacagaagaa ggtaagatga aatgtatagt ttcaataact ttcgataatt tgtttgtagt
      181 gcatgatata aaagttatag aaggccataa tgggctattt atagcaatgc ctagcagaaa
      241 agtaggcgaa ggaaatttca gagatatagc tcatccaata aatgcagaaa tgagacaagt
      301 tttagaagat gcagttttac aagcatatca tgaagcatta gttcaatggg aagtagctgc
      361 tgaataataa atatataaaa tggagagagc ccgaacattt aggctctttt taaatgtaga
      421 atttctatta aaatatgtac tttttagtaa aaaagtgcta tattattagt gataatgtat
      481 atgttgtata caaattgtta gataaatgta ttataatgta tattgattaa gagaataata
      541 cgaataaaat taaattagat tgaatggagt gtatcaatga actttaaagc tataatcctt
      601 gctgctggca aaggaacaag aatgaagtct aaatacccaa aagttataca taaagtatgt
      661 ggaaaagaaa tggttaacca tattatagat gtttcaaaaa aatcaggggt aaaagatact
      721 gtcgtgattt taggacatga agctgaggta gttaaagaaa agcttgcaga agaaattata
      781 atagccatgc aaacagaaca acttgggaca ggacatgctg taaagatggc taaagaatat
      841 attaatgatg aagatacaat agttgtactt tgtggagata ctccacttat aaaagaagag
      901 acactgaaga gattatttga atatcatata gaaaataaat atcatgctac agttcttaca
      961 acaagagttg gaaatccaac aggttatggt agaataataa gagataagaa gggtgacctt
     1021 cttaaaatag tggaacaaaa agatgcaaat tcagaagaaa agatgataag tgagataaac
     1081 tctggaattt attgttttaa tggaaagagt cttagagaag cactagattt gctaaataat
     1141 aacaattcac aaggtgaata ttatttaact gatactgcta aaataatgag agataaagga
     1201 ttaaaagttg gtgcatttgc aggttcaact attgaagaac ttatgggcgt taattcaaga
     1261 gtagaattat caaaggcaga agaaattatg agacgaagaa taaatgaatc tcatatggta
     1321 aatggagtaa ctataataga cactaactct acctatatag aatctgatgt tatgataggc
     1381 aatgatacta taatatatcc aggtgttatg ttacaaggga aaacaaggat aggttcagat
     1441 tgtataattg gaatgaactc atctataaca aattcagaaa taggtgatgg tacagaaata
     1501 aaaaattcaa ctataattga tagtaaggtt ggagaaaatt ctaatgttgg gccatatgca
     1561 tacttaagac caaagagtga cttaggaaat aatgtaaaaa taggcgattt tgtagaagta
     1621 aaaaatgcta ttatagaaga tggatcaaaa gcatctcacc tttcttatat aggagatgca
     1681 catgtcggta aaaatgtaaa tattggatgt ggagtagtat ttgtaaacta tgatggtaaa
     1741 aataaattta aatcagtagt aaaagacaat gcgtttatag gttcaaattc taatttagtt
     1801 gcaccagtag ttgtagaaga aaaaggatat atagcaacag gttcaacaat aacacatgat
     1861 gtcccagatg gtgctttggc aatagcaaga gaaagacaag ttatcaaaga aggatgggta
     1921 gaaaaaaaga atcaaaaaga cgaccaaagt aagtaattat gtagtttatt attttggcta
     1981 aaaaatttag ctcgaaatta ttataaatta tattcattag tattttaaaa ttttcaggag
     2041 ggttacataa atgaatacta gcggaagcga gattaaaatt attgcaggta attcatcgaa
     2101 agagttagca caaaaaatag ctgactatat aggtgtatca gtactagatt gtgaagtagg
     2161 tacatttagt gatggtgaaa tatgtgtaaa catgaatgag acagtaagag gttgtgatgt
     2221 gtttgtagtg caatctacta acagcccagt aaatgataac ttaatggagt tattaatctt
     2281 aattgatgcg ttaaagagag catcagcagg tagaataaca gcagtcattc cttactatgg
     2341 atatgcaaga caagacagaa aggctaaggc aagagatcca atcactgcta aattagttgc
     2401 aaatttaata actgctgctg gagcagacag agtattaaca atggatttac atgcagctca
     2461 aatacaagga tattttgata taccattaga ccacttacta ggtggaacaa tattagcaaa
     2521 ttattttaat gaaaagaaaa tagaagattt ggtagtagta tctcctgact taggaagtgt
     2581 tactagatca agaaaatttg ctaatacttt aaatggagaa gttccaatag ctataataga
     2641 taaaagaaga ccgaaagcta atgtatgcga agttatgaac ttaataggtg atgttaaagg
     2701 taaaaatgtt atactattag acgatatgat agatacagca ggaacaatag ttaatgctgc
     2761 aaatgcactt aaagagtttg gagcaaaaga tgtttatgct tgttgtactc atggtgtgtt
     2821 atcaggtcca gctattgaaa gaattgctaa ttcagaaata agtgagttaa tagttcttga
     2881 tactatacaa cttcctgaag agaaaagaat agataagata aagataaaaa cagtagctcc
     2941 tttatttgga gatgctataa gaatgatatt ctctaatgaa tcagtaagta aattattcta
     3001 atttattgta tttatgagag ttgtttaaaa ggatataaac cttttgagca actctttttt
     3061 gattttatac ttatggaatg ttattttttt cttgttagaa aggtatatat tattgtaaaa
     3121 agagattata gtttaaaatt atgtatattt aagtaaaata aacttaataa agggcatatt
     3181 attcaaaata ttatgaatat aaaaaatatt acaaaaaagt attgaaaaaa ttcagattat
     3241 gtagtattat gtaaatataa ataaagaaag aggtgtttcc aatgggaagg ttagaagatg
     3301 aacccctaat aattaaatga ttcagtcaca atctaaagtg aatataaaaa tctattgaaa
     3361 gtttatttat aagaattgaa aatagtatct attctatctt tagtgtttgt gagagggttc
     3421 tggattatat aagattactg taaagagtaa ttttgcacaa taagcatagc tgtaaatgaa
     3481 gagtttcgtt tatataatga ttgtggctga atcataacta taatagatag aatgtttata
     3541 atagatgatt ttataaaata ctatattgta atagctttta gctttcaaat aaatattagc
     3601 aaaggatgga aatgacaatg ataaaactaa atgataattt ataaatatct ccttacgata
     3661 tataataatt gtagggagat gtttcttttt tttgtagagt ataatttttc tagaaattct
     3721 gtttcaatat gtaaaaagtt ggtatatata aatagtataa acagtgttgc taaaatatgg
     3781 tagatattat ataatataag tggatatgta tatacattat gagttatata attttgaatt
     3841 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttagag catattgagt
     3901 tagtggtgca accggctatg aaattgtatt tatttataga tactatcatt ttcatagctt
     3961 tttttattta aaaaatggtt atgtgttaga aataaatttg gttagtacgt ttaaataatt
     4021 ttacttagat ttttaaccag atatattata agtaagtatt aattaaaaaa tttaaggggg
     4081 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
     4141 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
     4201 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
     4261 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
     4321 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
     4381 gattaaagtt ggtaataaat tggtattaca gataggtaca aatgatgaag gagttacatt
     4441 aacagaagca caatcagcaa aattattgag tgatataggt gaaaataaaa tatatacaag
     4501 tgttacagca gataatttgg gaaatccatt aacaagtaat acaaaagtag ataataaagt
     4561 tctatatata gtacttatag ataatactgt gatggactca acaaaatagt taaaactata
     4621 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
     4681 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
     4741 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
     4801 aggatatata acagaagacc agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
     4861 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
     4921 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
     4981 agctacaaag atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
     5041 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gacagagaag cagttcaaga
     5101 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
     5161 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
     5221 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
     5281 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
     5341 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
     5401 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
     5461 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
     5521 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
     5581 gatagctatg agggttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
     5641 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
     5701 ggttgttggt cctactggaa gtgggaaaac cactacattg tattcaatgt taaatcaact
     5761 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
     5821 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
     5881 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
     5941 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
     6001 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
     6061 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
     6121 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
     6181 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
     6241 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
     6301 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggcatggtta gcttaaaaga
     6361 ggatatagtt aaaaaggttt taaatggaaa gacaacagta gaagaaatga ttagaataat
     6421 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
     6481 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
     6541 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
     6601 ttatctagta gaaagagaaa aaaagacttg ggagctgacc agataagcca cttttgtaga
     6661 cagtttgcta ttatagtatc ttctggtata aatagtattt caggattaga aacattagct
     6721 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
     6781 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
     6841 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
     6901 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
     6961 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
     7021 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
     7081 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
     7141 ttatttatct atcagtttaa aaaatattta aaaacgtcag ttggaagggc tcataaagat
     7201 agagttataa acaagatacc tgttttagga aaaggaataa attgtatggt agctacacga
     7261 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
     7321 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
     7381 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
     7441 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
     7501 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
     7561 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
     7621 tttatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaataagg
     7681 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
     7741 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
     7801 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
     7861 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
     7921 atatggacaa aaaggattta gatggattta tgaagctaga gatggacgaa atgatggctt
     7981 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacat gataattcag
     8041 atggcgatga atcaagctta aatcttatca ttggagctat acaaaaggaa gaggtaaatg
     8101 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
     8161 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
     8221 gtgacaatag tacaattata aatatatata agaaagatac tttatttatt agtgataata
     8281 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
     8341 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
     8401 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
     8461 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
     8521 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
     8581 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
     8641 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
     8701 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
     8761 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
     8821 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
     8881 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
     8941 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
     9001 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
     9061 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
     9121 cattaactgg aaatagtgag actaatagta ataacacgga taaagatgat aaaactccat
     9181 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
     9241 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
     9301 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
     9361 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
     9421 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
     9481 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
     9541 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
     9601 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
     9661 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
     9721 gacttaacta gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
     9781 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
     9841 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
     9901 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
     9961 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    10021 ttgataaaag aaggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    10081 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    10141 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    10201 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    10261 ccatttaagt ctagtgctaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    10321 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    10381 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    10441 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    10501 atttggaaaa ctgtaaagag ctaactaaaa ctggttcagg taataattat gaatacaata
    10561 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    10621 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    10681 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    10741 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    10801 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    10861 tgtcaagaat agggtaataa gaatttatta aaaaataagt aggttgataa aatatgagta
    10921 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    10981 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    11041 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    11101 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    11161 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    11221 gtgacgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    11281 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    11341 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    11401 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    11461 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttgat
    11521 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    11581 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    11641 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    11701 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gaaatatatc
    11761 agatgaagat tttaataatc aagatgagaa aaatattgaa gcagaaagac ttggatatat
    11821 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    11881 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    11941 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    12001 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    12061 ggcaaagtta gtgaataatg atattctttt gaaaatgttt ttagaagaaa ataaaattga
    12121 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    12181 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    12241 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    12301 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaacc tattggaaat
    12361 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    12421 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    12481 tttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    12541 gtcttatgag aaaatacctg ttaaatatga taatatggct ttgataacaa cttttgacta
    12601 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    12661 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    12721 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    12781 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    12841 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    12901 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    12961 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    13021 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    13081 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    13141 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    13201 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    13261 agcgggggaa tctattttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    13321 tctaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    13381 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    13441 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    13501 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    13561 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    13621 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    13681 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    13741 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag taggggcagc
    13801 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    13861 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    13921 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacttgat
    13981 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    14041 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    14101 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    14161 caatggatat tataatcgtt aatctatatg tatttatagt gggtatagtg tttggaagtt
    14221 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttccc
    14281 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ctaagctatt
    14341 tgatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    14401 tggaattaat tacaggaatt ttatttttaa cagtatttat gttatatgga atatctacat
    14461 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    14521 accactttat aataccagat aaaatattga tttttggagc tatattttct atgattttta
    14581 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    14641 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    14701 gaggagatat caaactattt gcaatgctag gcctttacat gggtgttaaa aatggtcttt
    14761 tgacagcctt actaagcgtt tatgtaggag cagtctatgg aatctgtgta ataattataa
    14821 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    14881 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    14941 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    15001 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    15061 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    15121 agaaaagaaa aatttatata atacgttttt tatttacata ataactgtag ttttatctgt
    15181 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    15241 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    15301 agttagtggt ataatagttc aaggtataat ttttttggcc tttttcaata tagctcaatt
    15361 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    15421 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    15481 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    15541 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    15601 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    15661 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    15721 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    15781 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    15841 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    15901 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    15961 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    16021 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    16081 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    16141 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    16201 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    16261 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    16321 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    16381 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    16441 cgacaaatga tttttataac gagtagtgat ttagaagcaa agaaagtata cgaagattta
    16501 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    16561 ttggatgcaa aggacagaaa cgaagaagca aagaaattaa aagtattgtt aaaactagca
    16621 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    16681 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    16741 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    16801 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    16861 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    16921 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctagaga atttatttat
    16981 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    17041 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgcagag
    17101 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    17161 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    17221 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    17281 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    17341 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    17401 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    17461 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    17521 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    17581 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    17641 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    17701 gcgtctaaga ctagcaataa aaaagttaaa aaaggaaaga aaatagaatc ctttttagat
    17761 ttaagtgttg gagactatgt agttcatgaa aatagtggtg taggtagata cacagggatt
    17821 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    17881 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    17941 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    18001 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    18061 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    18121 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    18181 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    18241 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    18301 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    18361 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    18421 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    18481 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    18541 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    18601 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    18661 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    18721 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    18781 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    18841 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    18901 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    18961 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    19021 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    19081 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    19141 acagagtttg gttctggttt taaaatagca atgagagacc tcgaaataag aggagcagga
    19201 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    19261 aaaatgctta atgatgcaat taaaaaagtc aaaggtgaac ctattgtgga ggaaattgat
    19321 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    19381 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    19441 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    19501 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    19561 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    19621 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    19681 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    19741 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    19801 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    19861 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    19921 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    19981 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    20041 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    20101 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    20161 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    20221 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    20281 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    20341 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    20401 acttcagcaa gtgatggtgg taaattagga ttcttttcaa gggggcaaat ggttgctgaa
    20461 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    20521 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    20581 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    20641 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    20701 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    20761 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    20821 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    20881 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    20941 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    21001 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    21061 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    21121 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    21181 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    21241 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    21301 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    21361 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    21421 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    21481 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    21541 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    21601 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    21661 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    21721 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    21781 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    21841 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    21901 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    21961 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggcc tacttgctta
    22021 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    22081 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    22141 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    22201 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    22261 tagataatgt tattgtaata agaagactta tggaagcagg atttacatac aaagtggcaa
    22321 attcaatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    22381 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    22441 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    22501 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    22561 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    22621 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    22681 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    22741 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    22801 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    22861 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    22921 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    22981 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    23041 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    23101 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    23161 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    23221 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    23281 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    23341 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    23401 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    23461 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    23521 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    23581 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    23641 tcatatatag acttggatac aagcatgata ataactcagg tatatgataa atttatagca
    23701 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    23761 aatggagcag gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    23821 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    23881 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    23941 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    24001 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    24061 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    24121 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    24181 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    24241 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    24301 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    24361 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    24421 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    24481 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    24541 ataaatagat ttgactttat agaaaaaagt gcaatgaact tgaataaaaa attagaagac
    24601 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    24661 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    24721 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    24781 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    24841 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    24901 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    24961 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    25021 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    25081 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    25141 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    25201 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    25261 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    25321 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    25381 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    25441 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    25501 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    25561 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    25621 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    25681 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    25741 aagagggaat tttaaattca tcaattattt tgctataatc tttgatgtat tgttttggtt
    25801 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    25861 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    25921 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    25981 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    26041 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    26101 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    26161 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    26221 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    26281 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    26341 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    26401 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    26461 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    26521 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    26581 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattac tgatagcaga
    26641 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaatcggtta
    26701 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    26761 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    26821 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    26881 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    26941 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    27001 agaatttatt gtaggtgatt cagaaggaat taaatttaat aaaagtgaga atgtaggtgc
    27061 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    27121 tatgagttca tttatatatg atgagattga caaaacgtta aatcatataa aagacagagg
    27181 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    27241 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    27301 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    27361 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    27421 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    27481 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    27541 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    27601 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    27661 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    27721 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    27781 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    27841 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    27901 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    27961 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    28021 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    28081 atggccaatg tcaacaatag gatgtctgta cggacagaag aagctatatc aataagttta
    28141 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    28201 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc taccatggga
    28261 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    28321 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    28381 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    28441 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    28501 ttgccagaga gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    28561 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    28621 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    28681 gtgctagacc aaagagatat agctaatgta atagatatga ttcataatga tgaatgtaaa
    28741 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    28801 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    28861 aattttagaa aggaatgtat gaaaccagag tcgatagtga aaatatcaaa tcactattac
    28921 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    28981 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggt
    29041 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    29101 ataacagttg ataaagtaag ttacttaaca aaaagtaaca atgagtttga aatatcaata
    29161 gaaaagaaaa cttgtcatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    29221 gacctagtag gtgaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    29281 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    29341 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    29401 aaatacatga tggctataag tgatggtatg ggaaagggta aaaaagcata tgaagaatct
    29461 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    29521 attgatacta taaataatat gttgttgtta aaatcttcag aagagatgtt ttcaactctg
    29581 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    29641 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcatcatc atcattacct
    29701 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    29761 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    29821 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    29881 ttaatattgg atagagcatt agagttacaa gcgttacaaa tcgaagatga catgactgtt
    29941 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    30001 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    30061 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    30121 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    30181 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    30241 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaatttat
    30301 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    30361 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    30421 acagagcaat tgctgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    30481 aagtagacta tgaaaaaaca tgttggaatt tgcaatcttt gttcaaaagt gatgaccaat
    30541 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    30601 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaggaa aaacttgata
    30661 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcat
    30721 ataagtactt agatatgagt gatagtatga gtaaatcata tgggaattat atggggattt
    30781 gttcagattt agaattagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    30841 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    30901 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    30961 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    31021 cgcaatatgc aagtgaatta gagtcagtag atagagaaaa tagaaaaaaa gcttatcaag
    31081 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    31141 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtact
    31201 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    31261 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    31321 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    31381 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    31441 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    31501 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    31561 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatact
    31621 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    31681 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    31741 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    31801 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    31861 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    31921 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    31981 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    32041 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    32101 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    32161 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    32221 aatccacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    32281 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    32341 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    32401 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    32461 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    32521 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    32581 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    32641 agagccaaaa ggattgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    32701 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    32761 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    32821 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    32881 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    32941 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    33001 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    33061 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    33121 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    33181 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    33241 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    33301 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    33361 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    33421 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    33481 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    33541 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    33601 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    33661 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    33721 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    33781 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    33841 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    33901 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    33961 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    34021 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    34081 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaaaat agaaatcaaa
    34141 gaaatgacta taataagaca acgagtgtaa atgatagtaa taagtcatca aatatatcta
    34201 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    34261 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    34321 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    34381 atgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    34441 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    34501 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    34561 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    34621 atttaatttc acctatagga aagggacaaa gaggattgat agtagcacct ccaaaggctg
    34681 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    34741 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    34801 ttgaaggaga cgttatatat tcaacttttg accaagtatc aagtcatcat gtcaaggttg
    34861 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    34921 tactagatag tataactaga ttggcaaggg catataacct tacaatatct cctacaggta
    34981 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    35041 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    35101 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    35161 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    35221 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    35281 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    35341 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    35401 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    35461 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    35521 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    35581 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    35641 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    35701 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    35761 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    35821 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    35881 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    35941 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    36001 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    36061 aggttctttt gttatgatag atacaatgat tcaagggata aaaagtttaa acttttcttc
    36121 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    36181 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    36241 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    36301 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    36361 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    36421 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    36481 atttaaaaga ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc
    36541 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    36601 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    36661 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    36721 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    36781 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    36841 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    36901 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    36961 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    37021 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    37081 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    37141 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    37201 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    37261 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    37321 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    37381 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    37441 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    37501 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    37561 aatattatga aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt
    37621 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    37681 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    37741 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    37801 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    37861 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    37921 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    37981 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    38041 tggagctgga ggagatgaag cagctctatt tgcgggtgat ttatttagaa tgtactcaag
    38101 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    38161 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    38221 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    38281 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    38341 tccaaatgat ttaagaatag atgttttccg ttcttcgggt aatggaggac aaagtgtcaa
    38401 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    38461 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    38521 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    38581 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    38641 tgaccataga ataaatttaa ctttatataa gttagatgca tttttagatg gagatataga
    38701 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    38761 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    38821 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    38881 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    38941 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    39001 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    39061 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    39121 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    39181 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    39241 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    39301 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    39361 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    39421 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    39481 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    39541 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    39601 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    39661 aaaacttctt agagaaggta aaacagttat ttttccaact gagacagtat atgggttggg
    39721 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    39781 tgacaatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    39841 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    39901 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    39961 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    40021 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    40081 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    40141 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    40201 taaagaagtt ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa
    40261 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    40321 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    40381 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtatcagta gaaaccgaga
    40441 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    40501 gtttgatgct cttatagaaa tggacaaaaa gggcgttgat gtcatatatt cagaagagtt
    40561 ccctaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    40621 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    40681 atacttgtag aagccctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    40741 gaagtattga agaatattcg atatcatcag caggtatatc tacagcaaat ggaatgggtg
    40801 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    40861 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    40921 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    40981 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    41041 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    41101 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    41161 aaaaaagaaa taataagcta tttagaaagt aaaggtatag aatgtgtaga ttatggaact
    41221 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    41281 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    41341 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    41401 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    41461 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    41521 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    41581 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    41641 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    41701 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    41761 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    41821 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtaagcttaa
    41881 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    41941 ttgaatatta ttgcaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    42001 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    42061 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    42121 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    42181 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    42241 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    42301 aggttctttt ttatatattt tttctcaaaa taggaattat gaattgataa atttctcgaa
    42361 ttgagaataa aaagttttta ccaattaaaa aagattgctt attttacaag gttttataga
    42421 atagtagtct attttaatgt ggcatgttag ttgcttacga ttaagattat aggtattaag
    42481 tgtattagtt aaattgagaa ttgaatatta ataatattat tgggagtggt aatacatgaa
    42541 gaattgtata gcagtaagag gggctggaga tttagcaaca gcagttattc ataaattaca
    42601 caacagtggt tttaaagtag ttgcgttaga aatagaagag ccattagcta taagaaggca
    42661 aatttctttt tgtgaggctg tttatgaaaa aaaagtaact gttgaaggtg tagaatgtat
    42721 attttgcaat actgaaaaag aaatagaaga agttttatct caacataaag tagccttgat
    42781 tatagaccca aaaggagaaa aattatctaa gttaaatcca atcgttttgg ttgatggaat
    42841 actcgctaag aaaaacttag gtacaactaa agatatggca ccacttacaa tagctttagg
    42901 tcctggattt tgtgcaggag aagatgtaga tgttgttata gagacaatga gaggtcataa
    42961 tctaggaaga ataataacta atggtagagc agaaaaaaat acaggagtac caggagcaat
    43021 aaaaggtttt tctaaagaga gagttatgca ttcacagtat gatggtataa tggaaaatgt
    43081 atgtaagata ggtgatatag ttgaaaaggg agacctactt tgttatatta ataattatga
    43141 aaaaaaacat gaagttagag caacaatatc tggagtcctt agaggtttat taaaggatgg
    43201 agcaaaggtg aacaaaaatt taaaaatact agacatagac cctagaaaag aagaagttaa
    43261 gaatgctttt acaatatcag ataaagctag atgtatagct ggaggggttt tagaagccat
    43321 actaaacaat gggatttatc cccaaaatta atttatatta gataaagcta ttaatatttt
    43381 attaagatga tataataaat ttaatagatt aaagagagca tatgacgggt atttaaaatt
    43441 taaatggtaa aaatgctctt tttttattgg acattcaatt aacatgtgta tataatataa
    43501 aaataagtat ttatttaggt tgaaatctaa taaatatata ataccagtac ataagcaaag
    43561 aggagaaaag taaattatga gaccatcatg ggatgaatac ttcatggaaa ttgcagaagt
    43621 agtgaaaaag cgttcaactt gtattagaag acaagtagga gctgtaattg taagagacaa
    43681 acaaatatta acaactggat ataatggttc tccgagaaat cttgaacact gtgaaaatat
    43741 tggatgtaag aggcaggaat taaatattcc ctcaggagaa aggcatgaat tatgcagagc
    43801 attacatgct gaacaaaatg cgataataca agctgctcat aatggtatta gtgtagatgg
    43861 ggcaactttg tatgttacta caagaccttg tgttttgtgt gcaaaaatgt gcataaatgc
    43921 aggtatattg aaaattgttt atgaaggtga ttatccagat gatatgtcta cagaattatt
    43981 aaaagaagca ggaatagaac tcataaaatt ttagtggata aaatttatat gacaataaat
    44041 gtcaaattat gacactgaaa tacaatggta tcaaatgatt aaaagaaagt atttgaaaaa
    44101 tcatcagatt agtagtaaga tatattttat aattaaataa atttatgaga tagattaatt
    44161 acatgttatt tattttataa taatgaggga tactatttat gagtaaaaga aatacacata
    44221 cagaaattgc aaagttgttt agtttaataa gtcaaatagg attgatgatt gtaatatcaa
    44281 tattaggatg tactttctta ggaaaatttt tagatagtaa attgaatact actccagttc
    44341 tgacaataat atttttattg ttgggtgttg gaggtgcttt tagtggtgtt tataaaactc
    44401 taatagtata tacacgaagg aagtgatata ttggatataa aattacttaa ccaggtaagg
    44461 tcagtaacaa aaggtatagt aatatttgat gtagttttaa ttgtgctaat gttgataaca
    44521 tcaacattaa gtaaaccagt attattagga cttatatttg gctctataat agctatgtta
    44581 aattttagat tattggcgat atcactggaa aaatcagtga catttcctgc tgggaaggca
    44641 caagcttatg catcagcaca atatgctatt agattattta taatggcagt tgttctaatt
    44701 gcatcagcta cagtaccaca tttaaatata atcggaacag cattgggatt attgagcaca
    44761 aagtttgtca tattatcaaa aaactttata aataaattaa agaggaagga ggcataagta
    44821 tgaatcaagc gcattattat ctcataggaa actttaagat tagtgaaaca atactagtaa
    44881 gttggggaat aatggccttt ttagcgatag catcttattt tttgactaga aacttaaaaa
    44941 aagttccaac tagtaaagtt caaatattct tagaatttgc agtaggtggt cttgctaaaa
    45001 tggttacaga tgttatgggt aaaaatacag taaaaagaat gccatatatg ataccataca
    45061 taggaagctt gtttttattt ttcgcatgtt caaatttaag cccattactt ggattaaggt
    45121 caccaacaac cgacttagac acaactgctg catgggcaat gattacattt tttatgattt
    45181 attttgcagg agttaaattt aaggggttat catactttaa agaattggct caaccacttg
    45241 ccataatgtt accaatcaat attataggtg agttagctag accaatatca ttaagtttcc
    45301 gtccatttgg gaatatttta ggtggaacaa taataatggg tctgttttat caacttttag
    45361 gttttatatc tagtttaata ccaggattat caataccaat tggagaaata ttaataccag
    45421 tcccattaca cttgtacttt gacttatttg caggattgtt acaagcatat atatttataa
    45481 tgttaacaat ggtatttata tcaagtgcag gagagttaga ttaagaaatc aacttatatt
    45541 aagataaaaa taaaataatt taaaaaatta aagttttaag gaggaaataa ttatggaaac
    45601 agcaatagta gcggcagcat cagcaatagg agcagggata gcagttgcaa caggtatagg
    45661 tgcaggtata ggtcaaggta tagcagcagc taaagctgca gaagcagtag gaaaccaacc
    45721 agaagctaaa ggtgatataa catcaacttt attactagga gttgctatag cagagtcttc
    45781 agcaatatat ggtttggtta tatcaataat acttctattc gtaaatccat tctttaaata
    45841 cttaggaatg tagatttagt ccttaaatat tttgagatag gtttataggt aaatcatctc
    45901 aaacttataa tcatgagatg aactttatca tctcgtgatt ataagtagtt atgatttatg
    45961 gaaaggagga taattatgga aaaagcacta gtaggtataa cgtgggaatt tgtgttccaa
    46021 attgttaata cttttataat atttttactt ctgagaaagc ttttattcaa gccagtatta
    46081 aatataattg aatcaagaga aaatgatatt aaaagtgatt tggcagaagg cgaaaaagca
    46141 aaaaatgaag gtttagctct gaaaaaagaa tatgagtcaa aaatcaattt tgccaaggat
    46201 gaaggtcaag aaatcataaa acaagcaact ataagagcag agcagaaatc agatgatata
    46261 gtaaatactg caaagaaaga tgctcttgat ataaaagaaa aagcaaacaa agatatagaa
    46321 caagaaagac aaaaggttat aaatgaaatc aaaaatgata tatctaacat agcactactt
    46381 gcagcatcta aagttattga aaaagattta gataaatcta aacatgaaga attgatagaa
    46441 aactttataa aagaggtagg ggaagctaaa tgataaatgt aatagcaaat agatatgccg
    46501 aagccctatt tcaattagga gaagaagaaa attcaactga tgtcttattc aaagaactag
    46561 aaaaagtagt agatatgatg actaaagtta gtaaagattt ctacaaagtt ttaaaatcac
    46621 cattagtttc aaaatcagaa aagaaaaact tggtagaaat tatttttagt aaggaagtaa
    46681 gttctaacat aaaaaacttt ttaaaagttt tagtagataa agatagaata tcatatttag
    46741 aagatatcga attagcttat aaagaattat taaataagaa aaacaatgta attgatggag
    46801 tagcaataag tgctattcca atgagtgaaa cagatattaa agaacttgaa gtcaaacttt
    46861 caaataaata caacaaaaat gttactatag aaaatgttgt tgacaagact atactagggg
    46921 gagttctagt tagaattgga aatgaacaaa tagatggcac tgtgaaaact cgtcttgata
    46981 agatgaaaga aaagctatct gaagtaatat cttaagaatg gcggtgaacc catgaactta
    47041 aaacctgaag aaataagttc tataattaaa cagcaaataa aaaattatga gaataaagtt
    47101 gagttgacag atacaggtag tgttttaaca gttggggatg gtatagcgag tgtatatgga
    47161 ttagaaaaag ctatgtctgg tgagttgcta gagttcccag gtgaaatata tggtatggca
    47221 cttaacttgg aagaagaagt agttggagct gttatccttg gagatgacag tgaaataaaa
    47281 gaaggcgata tagttaagag aactggaaga atagttgaag ttccagttgg agaagcttta
    47341 ataggtagag ttgtaaactc actaggtcag ccaatagatg gaaaaggacc tatagcgtac
    47401 acaaaaacta gaccagttga atctgaggca cctggtataa tagatagaag gtctgtttat
    47461 gaaccattac aaacaggaat caaatcaata gattcaatga taccaattgg tagaggtcaa
    47521 agagagttaa taataggtga cagacaaact ggtaaaacat ctatagttat tgacactata
    47581 ctaaatcaaa aaggaaaaga tgttatctgt atatatgtag caattggaca aaaacgttct
    47641 actatagctc aattagttag ctctttagaa aaaggtggag ctttggatta tacaatagta
    47701 gtttcagcaa cagcatctga atcagcacca cttcaatata tagcaccata tgctggagca
    47761 gctatgggtg aagaatttat gtacaatggg aaacatgtac ttatagtgta tgatgattta
    47821 agtaaacaag ctgttgcata tagagaaatg tcattgttac ttagaagacc accaggacgt
    47881 gaggcatatc ctggagatgt attctattta cattcaagat tacttgaaag agcagctaag
    47941 ttatctgatg aattgggtgg aggttcaatg actgccttgc ctataataga aactcaagct
    48001 ggtgacgttt ctgcatatat accaacaaat gttatatcta taacagatgg tcaaatatac
    48061 ttacaaccag agttattcta ttcaggtgta agaccagcag ttgaccctgg tatatcagta
    48121 tcaagggttg gtggttctgc gcaaattaaa gcaatgaaaa aagttgcagg aacattaaaa
    48181 cttgcatatt cacaatatag agaacttgca gcattctcac agtttggttc tgacttagat
    48241 gaagatacta aaaagagact tgctcaaggt gaaagaatcg ttgagatatt aaaacaaggt
    48301 gagcatcaac caataaaagt tgaaaatcaa gttatgataa tatatgctgt tataaataat
    48361 catttagagg atattccgat agataatata gctagatttg aatcagaatt gtatgcattt
    48421 gtagataata attatccgga aatatcaaga aaaatattag gtggagaaga cttcactcat
    48481 gatttaacag atgcaataaa tgaatttaaa gaaaagtttg ttgttgaagt ataatccttt
    48541 ttaaaaaagg aggtaaatta attggcagga gctggaatca aagcaatcaa aacacgtatg
    48601 ggaactgttg aaagtacaaa gcaaataact aaagcaatgg aattagttgc atcttctaag
    48661 cttagaaaag caaaagaaaa ggctgaagat tcaagagaat attttaatac tttatacaat
    48721 acagtaaaag atattgccca aacaacacgt ggaattagaa atgaattttt aaaagaaaga
    48781 gaagtaaaaa ataaatgtta tgtagtagta gctggagaca gaggacttgc aggtggatac
    48841 aattccaatg tattaaagga atctgtctct catatgaaag gtaaaaagga aacagtgatt
    48901 actgttggaa agaaagcaca tgaatttttt tctaaaagaa actacaatat agcaaaagat
    48961 ataaatagtg ttgaagcttg tggatatgaa gatgcgacag aaatttcaca aactattatt
    49021 gacctgtata aaaaaggtga agtagatgaa gtttatattg cttatacaaa atttaaatct
    49081 gctttatcac aagaagttga aattttgaaa ttgcttccat tagtctttga aaaagataca
    49141 gacgaacaag gaatggcagt aaaagaagaa aaacaaaata gatcaaaagt acaatatata
    49201 ccatcagcag aagcagtttt aagttatatt gtacctcagt ttatatcagg tacagtatat
    49261 ggtggaattt tggaatcttt tgcatcagag caaggtgcta gaagaactgc aatggaatca
    49321 gccacagaca atgcgaatga aatgctatct aatttagagt taagttataa cagagcaaga
    49381 cagtcagctg taactcaaga aataacagag atagttggtg gagttgaagc tctaaaatag
    49441 ggaggttagg aaatggcaaa tgtaggtaaa gtagtacaga ttgtaggagc tgtactagat
    49501 gttaagtttg atagtgaaca aagcctacct aacttattaa atgcgttagt aataaagtta
    49561 ggagataaag aaatagttgc agaagttgct caacatatag gtgatgatac tgtaagatgt
    49621 atagctatga gtgcaacgga cggacttgta agaggtatgg aagttgtaga tacaggaggg
    49681 ccaataagtg tccctgttgg agatgagact ttaggtagaa tattcaatgt tcttggaaaa
    49741 ccagttgatg ggaaaccagc tccaaaatct gcacctaaat tacctataca tagaccagca
    49801 ccggcgtatg atgaactaga aactacagca gagatactag aaactggtat taaggtagtt
    49861 gacttactag ctccatatct aaaaggtgga aaaataggtc tatttggagg agcaggagta
    49921 ggaaagacag ttctaataca agagcttata aacaatattg ctaagcaaca tggtggtatt
    49981 tctgtatttt caggagtagg agaaagaaca agagaaggta acgaccttta tggcgaaatg
    50041 agtgagtctg gagttataaa taaaacagct ctagtatttg gtcaaatgaa tgaaccacct
    50101 ggagcgagaa tgagagttgc tttaactgga cttacaatgg cagaacattt tagagatgag
    50161 caaggacaag acgttttact tttcgttgat aatatattcc gtttcacaca agctggttca
    50221 gaagtttcag cacttctagg acgtatgcca tcagctgttg gttatcagcc aacattagct
    50281 actgaaatgg gtgcacttca agagagaata acatcaacta agaaaggttc aataacatct
    50341 gttcaagcag tatatgtacc agcagatgac ttaactgacc cagcaccagc tacaacattc
    50401 tctcacttag atgcaaaaac agttttatca agacaaatat catcactggg tatatatcca
    50461 gcagttgacc ctcttgaatc tacatcaagg attcttgacc ctagtatagt aggaaaagaa
    50521 cattatgaag tagctagagg tgtacaatca atacttcaaa gatataaaga acttcaagat
    50581 ataattgcta tacttggtat ggatgaatta tctgatgaag ataagttaat agttgcacgt
    50641 gctagaaaga tacaaagatt cttatctcaa tcattcacag ttgctgagca atttacagga
    50701 aatccaggtc aatatgttcc tgttaaagag actgtaagag gatttaaaga aattcttgaa
    50761 gggaaacatg atgacttacc agagtcagca ttcttatttg taggaacaat agaagacgct
    50821 gttagaaaag cgaagggaag tatgtagtca tggccagtga attttcattg aaagttgtaa
    50881 ctccaagtaa tactttttat gatggagatg ttgaaatgat agtagttaga actacaaaag
    50941 gagacaaagg ggtgttgaaa ggtcatatac catttgtttc aggtatacct gtaggaactt
    51001 taaagataaa aaaagaggga agttttaaag aggcgaaaat agcagaagga ttcattaatg
    51061 taactcaaga taaaactgtc atattgacag aatctgctga gtggatttaa taaattaatt
    51121 tagagtataa aaagtcattt tgaccaaaaa aacttttggg taaaatggct tttttgtgtt
    51181 ttggtgttaa taaaataacg ataaaattgt aagtttttag tgataataaa ttcacaaaat
    51241 gtttaaattt actgccaaat tatgttgtaa ttaataagat aaagaagatt tagatagctt
    51301 ttatgtaagt gaaaaagtct tcatataaga aatacatata aaatttttat aaaatttaaa
    51361 aaatttgctt agttgtgtag tataataaat aatataatag atttgtatat aatattcatt
    51421 acaaataatt ttaaaagtta cagttttatt ataatgatta ttaaattaag ataagtaaca
    51481 taaactaaat aataatgtgt attatcgata gtaaatttat aaattcatta ttaattattg
    51541 aaagaagtat tataaatttt ctggaggaac aatgaatata tttgatattg gcgtagatat
    51601 aattgaaatt gatagaataa gaaaggctgt agacaaaaat aataggtttt tggagaagat
    51661 atttacagat agagaaatag agtattttaa ttctaagaat tttaaagcag aaagtatagc
    51721 aggtaatttc gcagccaagg aagctataag taaatctata ggtacaggaa ttagattgtt
    51781 taattttaaa gatatagaag ttctcagaaa tgaaatggga aaaccaatag taaaaactta
    51841 taataatcta gcaaaaatgt gcatagatta taatgtttta gaaataaaag tttcaatatc
    51901 acattcaaaa gattatgcta tagcaaatgc gataaccata attaaggact aaggagtgat
    51961 tacattatga tggataaaac agcaaaagaa ataatgacaa cagatgtaat agtagcaaaa
    52021 caagatgata gtatagcaga tgtagctaat atgctaattg cagaaaaaat aggaggtctg
    52081 ccagttgtag attcagagaa tagagttgtt ggaataattt ctgaaacaga tattcttaaa
    52141 aaagaaaaat atatagaagc acctctctat ataaatctat tacaaggatt gattttctta
    52201 gatgacttaa aaaaagttga aaaagatata aaacaagtag ctgcttataa ggttggagaa
    52261 cttatgtcta aagatataat aaaggttcat gaagatgata agtttgatga tgtagctaat
    52321 gtaatgatta aaaaatcaat aaacagagtt cctgtagtag atgatgacaa caaacttaaa
    52381 ggtataattt gtagatatga tataataaaa gctttataca atgaataaac tgaagtagga
    52441 ggtgtgatgt gagaaaaaag tggaccatag tatgtattat gttcttggca ctggttataa
    52501 ttgtaatagg atgccaaaaa cgacagtcca caaaagaaga agtatacaaa gattttcaaa
    52561 agcagatttc tgatatgaat tattacagtt gtaaagctga agtagaagta gttggaaata
    52621 aaagcccaca caattatgtt ttaattcata cttataaaaa aaccgataac tataaattag
    52681 aagtaatttc cccaaaacat ttaaaaggga aaagtataga atatcaaggt gataaaatat
    52741 tagttaaaaa tccaaagatt agtgatgtag tggagttacc taatacagga aaaaataatc
    52801 aatatctttt tgtaggagac tttataaaaa actatcttca aaatgaggag atgaaagtaa
    52861 agttatccaa aggacatttg gttttagaga cttttattcc tggggataat aaatatttta
    52921 acaagcaagt attatatgta aatgctgaca caaaaaatcc tgaaaaaatg gaagtgttag
    52981 ataaagaggg agtgccaaga tttacagtaa aatacaaaga ttttgaatac agaaactaag
    53041 ggggactaaa gacatgcaaa aaataacagt gcctacatgg gcagagataa atctagataa
    53101 cttaagattt aacttaaata atattaaaaa tttattagaa gaagatatta agatttgtgg
    53161 agtaataaaa gctgatgcat atggacatgg tgcagtagaa gttgcaaaat tgctagaaaa
    53221 agaaaaagta gattacttag cagtagcaag aactgctgaa ggaattgaac ttagacaaaa
    53281 tggcataaca cttcctattt tgaacttggg atatactcca gacgaagctt ttgaagattc
    53341 tataaaaaat aaaataacta tgacagttta ttctttagaa acagcacaaa agataaatga
    53401 aattgcaaaa tctttaggag aaaaagcctg tgttcatgtt aaaatagact cagggatgac
    53461 tagaataggt ttccaaccta atgaggagtc agtacaggaa ataatagaat taaataaatt
    53521 agaatatatc gatttagaag gtatgtttac tcattttgct acagctgatg aagtaagtaa
    53581 agagtacact tataaacaag ctaataatta taaatttatg tctgataaat tagatgaggc
    53641 tggtgtaaaa atagctataa aacatgtatc aaacagtgca gctattatgg attgccctga
    53701 tttaagatta aatatggtaa gagcaggaat aatattatat ggtcattatc catctgatga
    53761 tgtatttaaa gatagattag aattaagacc agccatgaaa ttaaaatcaa aaatcggaca
    53821 tataaaacaa gttgaaccag gtgtaggaat aagttatgga ctaaaataca caactacagg
    53881 taaagaaaca atagctacag ttccaatagg atacgcagat ggatttacta gaatccaaaa
    53941 aaatccaaag gttcttatta agggagaagt gtttgatgta gttggtagaa tatgtatgga
    54001 tcaaataatg gttagaattg acaaagatat agacataaaa gttggagatg aggttatact
    54061 atttggagaa ggcgaagtta cagctgagcg tatagctaaa gacttaggaa ctataaacta
    54121 tgaagtgtta tgtatgatat caagaagagt tgaccgtgtt tatatggaaa ataatgagct
    54181 tgtacaaata aacagttatt tgctaaaata aaaccatgat attataatct gggaggcgat
    54241 tttgtgtacg ataagagtaa agaaaaaata gaagttactt tacctgactc tctgatttct
    54301 gaagttgata gtatagttca gatggagaac agtaacagag aagattttgc aaaagctgct
    54361 tttcagttct atataactca gaaaaagaaa attcatatta aagaatctat gaaaacgggt
    54421 tatagagaaa tgggtcaaat taatctgtca ctagctgaat taggtatgac agttgaagaa
    54481 gtatcttctg atgataaaaa tgaagggaag attgcccaag gtgagtacta attgtgaaat
    54541 aaagcgtggt gatttatatt acgctgattt aagtccagtt gttggttcag agcagggagg
    54601 cgttagacct gttctgataa tacaaaatga tgtaggtaat aaatatagtc ctacagttat
    54661 agtatcagct ataacatctc agattaataa agccaaatta cctactcatg tagagataag
    54721 ttccaatgaa tatgggctta ataaagactc agtaattctt ttagagcaaa ttagaactat
    54781 cgataaaaag agactgaggg aaaaaattgg ttgtctagat gaaaatatga tgataaaagt
    54841 agatgatgga cttcaaataa gtttaggatt gtttactttt taaagaggga tttgtctttt
    54901 attagacaat cccttttttc ttttaaatat ctcttaaggt ttaattttaa tgtactatgt
    54961 taataaaatt aaaaataacg caaaaaaata gcataaaagt gatataataa tatggaattg
    55021 atataaaatt acgaactcaa gggaggtaat tgcatgagag accataaagg attaaatgag
    55081 attgcgcgta tcataagaag agatatagtt tcaatgatac atagagccaa atctggacat
    55141 ccaggagggt cattatcagt agttgaaata cttactgcgt tatactttga tgaaatgaat
    55201 gtggattctt caaatcctaa aatggaggat agagatagat ttgtattatc aaaaggacat
    55261 gctgcaccag cattatatgc tacattggct gaaaaaggct attttgataa ggaagaactg
    55321 aatggattaa ggaagatagg aaggatgtta caaggtcatc cagacatgaa aggcacgcca
    55381 ggtgtggaaa tatctactgg ttctttaggt caaggttttt cagtagcatg tggtatggct
    55441 atggcatcaa agttggataa tgctccgtgg aatgtgtata ctttgcttgg tgatggtgag
    55501 gttcaagaag gaattgtttg ggaagctgct atgagtgctg cacattataa acttgataat
    55561 ttaattgctt ttttagataa taatggactt caaatagatg gagatataga aagcgtaatg
    55621 agtttaggtt caatagttga taaatttaaa gcttttggtt ggaatgtaat tgagatagat
    55681 ggacatgatt ttgaccaaat atttgcggct cttgatatag ctaaatcaac agttaaaaaa
    55741 cctacaatga tagttgcaaa aactataaaa ggcaaaggaa tatcttttat ggaaaatcaa
    55801 gcaggatggc atgggacagc tccaagtgat gaggagctag aaaaagcctt gcttgaatta
    55861 ggaggtgctg acaatgagta aaatggcgac gagagaagca tatggtaaag cattagtaaa
    55921 attaggtcaa ataaatgaca atgttgtagt attagatgca gatttatcaa agtctacaaa
    55981 aacacatgat ttttacaagt catttccaga tagatttttt aatatgggta tagctgaaca
    56041 aaatttaata ggcgctgctt gtggattatc aactgctggt aaaatacctt ttgcaagtac
    56101 ttttgctatg tttgctacgg gaagggcttt tgagattata agaaattcag tttgttatcc
    56161 aaagctaaat gtaaaaatat gtgctactca tgcaggtctt actgttggag aggatggagc
    56221 atctcatgaa agtgtggaag atattgctat aatgagagct ataccaaata tgacagttct
    56281 tgttccagct gatggagttg aaacagaaaa aattattttt gaaatagcta agtataatgg
    56341 acctgtttat gttagattag ggagaagttc agtaccagtt ttatttgatg aagattataa
    56401 atttgaaata ggaaaaggta cagtcttaag agaaggaaag gatgtatcaa ttatagcttg
    56461 tggaattatg gtaaatgaag ctctattagc acaagaaaag ttgcaagaag aaggtataaa
    56521 tgctagagtt ataaacatgt cctcgataaa acctatagac aaagatttaa tattagaatc
    56581 agcaaaagaa acgaatgcta ttgtaactgt agaagaacat agcataattg ggggattagg
    56641 ttctgctgta agtgaggtag taggagagtc ttgtccaacc attgtaaaga aagttggaat
    56701 taaagataca tttggagaat ctggtactcc aaatgaattg ttaaaaaaat atgaattaac
    56761 ttgtgatgac ataataaaga ctgttaaaga agctattatt gcaaaaagaa tgtaagagat
    56821 ataaattatt catgtttgtt aataatataa tatatttaaa agagaggtat aaagtgttat
    56881 attaatttat atctctcttt tttttatatg atatcaatat atcaaaaatg taataaaata
    56941 ttcaaatttt tacaaaaata gagaaaatat tcaaatatat attgactatt cagaaaaata
    57001 tgactaatat ataatctata gagaaactaa ttttatatat aatgactact tagataaaag
    57061 aatctaaaaa aattatgaga ggaggagtag ggtataaaat cagattataa gttttaagtt
    57121 attatactaa aaagctataa aacagttatc atgtatttta tgttaaagga aattgtaggt
    57181 agtttatcga attatttaat cagaagaaaa aatttcacat ggggaggtaa ctgagatatg
    57241 tcagaaaaag taaatgttaa aacagtcatc agtttcgcag gtgcgtatgt agcaacagta
    57301 atcggttctg gatttgcaac aggacaggaa ataatgcaat tcttctcttt ttatggtttt
    57361 gcaggtatta ttggtgggat aatttcaatg gtattattct catggatggg tgcttcagtt
    57421 atgtcaaaag gtaaagagct tcaacttaaa gaacctatca aaatttatcg tgtttattgt
    57481 ggtaagtatt tagggatatt ctttgaatgg tttggacctt tattcttatt tggtgttttc
    57541 gtagtaatga tttcaggagc aggagcaact ttaactgaat actatggatt aaatccattt
    57601 gtaggtagag taggtatggc agtagtagca ttattaactg tttcactagg tcttgataaa
    57661 ttatcaaaga tactaggtgg aatagggcct ataataataa tctttacact tttagttggt
    57721 ggtataagtt tagctaaaaa cataggtaat ataggagaag ctgcacaagt tcttagcact
    57781 gtaaagatag ctaaaccagt tccaaatgcc tatctatctg gtgtaatata tactacttat
    57841 aacacaattg ttgttatggc attcttaact ggattaggag caagtgctgc taacaaaaaa
    57901 gaagctattt gtggtggtat attaggtgga gtagcactta tggctgcagc tataatgatg
    57961 cacttagcta ttctttctga tataggaaac ttatattcaa aagcaatacc atcattattc
    58021 ttagcagata agatttcacc agtaattgga gttttattct caataattct tatattaggt
    58081 atatatacaa cagctgtacc tttattatgg tcagtaacta atagatttgt agatgataaa
    58141 catcctaagt ttaaattaat aactttaata gcagctatat taggtcttat aggtggcttc
    58201 ctaccatttg ataagctggt tggtatttta tatccatata ctggatatat gggcgtcata
    58261 atactggttt gtgttcttta tagacagatt acaaaaacat ctggatatca agaaggaatc
    58321 agtgacaaat aagtattttt taatattgta aactcaagag ctatatgaaa acaatgaaaa
    58381 tgttttcata tagctctttt tattttttag ctcgtttatt atgaaatagg aaaaacatgt
    58441 tttttagtta cattacatat atttataatt atggataaat atatactaat caataattta
    58501 gagtctaaga taagcttata aatcatatat ttagaatata tgggaggagt gataagattg
    58561 aatgtaaaat ttaatattaa aggtataatt tatggagtta tagctcttgt attcgttata
    58621 gggggattta tattcatacc aagtatgttt gtagagaaat ctaaacccat agattataca
    58681 gtattacaaa gaaattcaat accagaaaag atattagatg tgatggataa gtatacaaat
    58741 gaagaaagag cattagcggc taaaatcgat aacaaaatat acattatagt gactagagga
    58801 aataataaat atggtataga aatggacaaa atagaaagtg tggttgagga aggaaaggat
    58861 gttttgagag tcaagataaa gtataaagat aaggaagatt cttttccata catagttgtt
    58921 gaaacaaata tgagtgaact tccagataga atagaattaa atgcgacgaa ataagaaggt
    58981 attttataat taaataactg catattgtac aataaagtca tggtttttat taatgtaagt
    59041 aaattgtata tggttcttat tgttgtaaaa gccatgactt aaaatgctta taaagtaaga
    59101 tattaacagt agtatttatt actatattaa atggggtctg gaaatactta atgttctaat
    59161 tatttggagt gataattcta gtttcagtaa ctatgtagtc taattgtgca tcatgagttt
    59221 cttttggaat agaatcgaat atctggaagt caaatgctat gccaatagtt atagcatctt
    59281 ttcttaagtt ttctaaaaat ctatcataaa atcctccacc atatcctaat ctatagcaat
    59341 gtatatcata tgctacagct ggtacaatta caacatctaa ggccttaata tcaattgtag
    59401 gtgatttttc gttaggttct cttattccat atgctccaat tctcaatccc tcttttaaat
    59461 ctgttatttg agtagggatt aatgtatgat tttcttttat tgtgatggga cttgatacta
    59521 ttttctttag agacaaaagt tttttgatta aattatctgt ttgaacttca ttgttaaaat
    59581 ttagataaag cattatatta gttgcatttc ttatatcttc catttgaagt aatttttcag
    59641 ttattaaatt tgaattacta agtacaaagt ttgcactctt attttttctt gattcaataa
    59701 ctttttttct aaattctttt ttcataaaat aattcctccc ttaatatgtt ataatgtgta
    59761 cagcaattgt atttaatata aattttagta taatttattg taataaagca atatatttgg
    59821 gtaaatgtaa taaaaataac agctagtcat ataataataa tagtttatgt aaaaggagat
    59881 gtatcttgtg atttcaaaga agaaagctat ttttttaggg gtgattttag taattataac
    59941 agctatggtt acatcagcat ttcagttgac attggggaat aaagttgtaa tatcgaaaga
    60001 actctatgaa gactataaaa aatatgataa gttgttagga ctagaatcta ttataaaaca
    60061 agatttttat aaaaaagtat cagatactga tttggtaaat ggagcagcaa aaggtctatt
    60121 tctaggaaca aatgataagt attctggtta ttatactaaa gatgaaatgg agaatctaat
    60181 taatgatagt gaaggttctt atgttggtgt aggtatgtat ataggggcat caaaagatgg
    60241 tggcttagta gtagttccca tgaaagattc tccagcagag aaagcaggtg taaaatcagg
    60301 ggataaatta gtaaaagtca atggaaaatc tgtctcatat aaaaattcag atgaagctgt
    60361 tcgtatgatg aagggtaaaa aagggaaaac agttgaactg actatattaa gagaagataa
    60421 acaattgaac tttaaagtaa aaacagacca gataattgag aagagtatag aaagtaaagt
    60481 aattgataat gatttaggat atatagaaat tacccaattt atatcaagta catatactga
    60541 ctttgataaa gctttaaaag aattaaaagc aaaaaatatc aagggattag taatagacct
    60601 tagaaataat cctggtggaa tgctagatat atgtaaagaa gttgctgatg agttaattgg
    60661 tgaaggaact atagtatata caaaagataa taagggaaat actgagtact tgaaatctga
    60721 taaagaaaaa ttgggacttc caatagtagt gttaactaat ggagaaagtg cttctgctgc
    60781 tgaaatatta acagctgcaa tcgttgataa taaagaaggt atttctgtag ggactacaac
    60841 ttttggtaaa gggttggtac aatcagttgt aagattgaag gatggaacag gatacaagtt
    60901 aactactgct caatatttta cacctaatgg agattatatc aatgaaaaag gcataaagcc
    60961 tactattgaa gaaaaagatg aaaataaaca gttagatgta gcgactaaat ggcttagaga
    61021 acagattgat aaataaagtt taatttttat aaacttatat tgtaaaataa agaaccttaa
    61081 taaaaggagt gtctcaaaat taacttttta gtttatgagg cactcttttg tgggtcttta
    61141 aaataaagtg tgtattcttc taaaaagtag tgataattaa aatattaaat atcactatat
    61201 ataaggaagg tgcacacttt ttatgtttaa caaaaacgaa atattaaaaa aacttattga
    61261 agaatatgat gttaaaacaa ctacagatat tcaagatatg ctaaaagatt tatttgcaag
    61321 tacaattcat caaatgttag aagcggagct tgacgaccat ttaggttatg atagatatga
    61381 caataagaat aaaaatacta aaaactctag aaatagatat agaaataaaa atgttaaatc
    61441 agattttgga gaaataactt taaatattcc aagagatcga aatggagatt ttgaaccaaa
    61501 ggtaattaga aattatgaaa atgatatatc aggaatagag aaccaagtga ttggaatgta
    61561 ctcaaaaggt atgtctactc gagatatatc aagccacctt aagagtattt acggaattga
    61621 tatatctcat tctctaatag ctaaaattac tgatagagtt ctacctttag ctcatgaatg
    61681 gcaaagcaga gctcttgata tggtttatcc aattgtattt atgggtgcta tacattataa
    61741 agttagaagt gaaggtagaa taataaacaa agctgcatat atagccatag gtgttaactt
    61801 agaaggtata a
//
LOCUS       DAEPOG010000005        56510 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEPOG010000005 DAEPOG010000000
VERSION     DAEPOG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.005"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            448..585
                     /gene="rpmH"
                     /locus_tag="KR432_000252"
     CDS             448..585
                     /gene="rpmH"
                     /locus_tag="KR432_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH0638814.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            663..1007
                     /gene="rnpA"
                     /locus_tag="KR432_000253"
     CDS             663..1007
                     /gene="rnpA"
                     /locus_tag="KR432_000253"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH0638815.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            1039..1290
                     /gene="yidD"
                     /locus_tag="KR432_000254"
     CDS             1039..1290
                     /gene="yidD"
                     /locus_tag="KR432_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH0638816.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            1322..2029
                     /locus_tag="KR432_000255"
     CDS             1322..2029
                     /locus_tag="KR432_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0638817.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            1998..2627
                     /locus_tag="KR432_000256"
     CDS             1998..2627
                     /locus_tag="KR432_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBH0638818.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            2739..4118
                     /gene="mnmE"
                     /locus_tag="KR432_000257"
     CDS             2739..4118
                     /gene="mnmE"
                     /locus_tag="KR432_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH0638819.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            4213..6108
                     /gene="mnmG"
                     /locus_tag="KR432_000258"
     CDS             4213..6108
                     /gene="mnmG"
                     /locus_tag="KR432_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH0638820.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            6101..6820
                     /gene="rsmG"
                     /locus_tag="KR432_000259"
     CDS             6101..6820
                     /gene="rsmG"
                     /locus_tag="KR432_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH0638821.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            6995..7780
                     /gene="noc"
                     /locus_tag="KR432_000260"
     CDS             6995..7780
                     /gene="noc"
                     /locus_tag="KR432_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBH0638822.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            7915..8688
                     /locus_tag="KR432_000261"
     CDS             7915..8688
                     /locus_tag="KR432_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH0638823.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            8690..9553
                     /locus_tag="KR432_000262"
     CDS             8690..9553
                     /locus_tag="KR432_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH0638824.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            9586..10728
                     /locus_tag="KR432_000263"
     CDS             9586..10728
                     /locus_tag="KR432_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0638825.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(10862..11437)
                     /locus_tag="KR432_000264"
     CDS             complement(10862..11437)
                     /locus_tag="KR432_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBH0638826.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            11697..12989
                     /locus_tag="KR432_000265"
     CDS             11697..12989
                     /locus_tag="KR432_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0638827.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            13094..13696
                     /gene="yedF"
                     /locus_tag="KR432_000266"
     CDS             13094..13696
                     /gene="yedF"
                     /locus_tag="KR432_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH0638828.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            13769..14032
                     /locus_tag="KR432_000267"
     CDS             13769..14032
                     /locus_tag="KR432_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBH0638829.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            14047..14232
                     /locus_tag="KR432_000268"
     CDS             14047..14232
                     /locus_tag="KR432_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBH0638830.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            14335..15531
                     /locus_tag="KR432_000269"
     CDS             14335..15531
                     /locus_tag="KR432_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0638831.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            15745..16023
                     /locus_tag="KR432_000270"
     CDS             15745..16023
                     /locus_tag="KR432_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH0638832.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            16060..16494
                     /gene="ssb"
                     /locus_tag="KR432_000271"
     CDS             16060..16494
                     /gene="ssb"
                     /locus_tag="KR432_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0638833.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            16513..16740
                     /locus_tag="KR432_000272"
     CDS             16513..16740
                     /locus_tag="KR432_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH0638834.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            16883..17203
                     /locus_tag="KR432_000273"
     CDS             16883..17203
                     /locus_tag="KR432_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0638835.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            17211..18167
                     /locus_tag="KR432_000274"
     CDS             17211..18167
                     /locus_tag="KR432_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBH0638836.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            18178..20172
                     /locus_tag="KR432_000275"
     CDS             18178..20172
                     /locus_tag="KR432_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBH0638837.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            20173..20622
                     /locus_tag="KR432_000276"
     CDS             20173..20622
                     /locus_tag="KR432_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH0638838.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            20641..21969
                     /gene="dnaB"
                     /locus_tag="KR432_000277"
     CDS             20641..21969
                     /gene="dnaB"
                     /locus_tag="KR432_000277"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH0638839.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            22174..22482
                     /locus_tag="KR432_000278"
     CDS             22174..22482
                     /locus_tag="KR432_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638840.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            22564..23853
                     /locus_tag="KR432_000279"
     CDS             22564..23853
                     /locus_tag="KR432_000279"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH0638841.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            24179..24254
                     /locus_tag="KR432_000280"
     tRNA            24179..24254
                     /locus_tag="KR432_000280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24212..24214,aa:Lys,seq:ttt)
     gene            24261..24335
                     /locus_tag="KR432_000281"
     tRNA            24261..24335
                     /locus_tag="KR432_000281"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24294..24296,aa:Glu,seq:ttc)
     gene            24340..24415
                     /locus_tag="KR432_000282"
     tRNA            24340..24415
                     /locus_tag="KR432_000282"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24373..24375,aa:Val,seq:tac)
     gene            24449..24523
                     /locus_tag="KR432_000283"
     tRNA            24449..24523
                     /locus_tag="KR432_000283"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24481..24483,aa:Thr,seq:tgt)
     gene            complement(24906..25898)
                     /locus_tag="KR432_000284"
     CDS             complement(24906..25898)
                     /locus_tag="KR432_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0638842.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(25907..26965)
                     /locus_tag="KR432_000285"
     CDS             complement(25907..26965)
                     /locus_tag="KR432_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH0638843.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            27090..28553
                     /locus_tag="KR432_000286"
     CDS             27090..28553
                     /locus_tag="KR432_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBH0638844.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            28655..29452
                     /locus_tag="KR432_000287"
     CDS             28655..29452
                     /locus_tag="KR432_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0638845.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            29465..31567
                     /locus_tag="KR432_000288"
     CDS             29465..31567
                     /locus_tag="KR432_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBH0638846.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            31635..32114
                     /gene="rlmH"
                     /locus_tag="KR432_000289"
     CDS             31635..32114
                     /gene="rlmH"
                     /locus_tag="KR432_000289"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH0638847.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            32263..32574
                     /locus_tag="KR432_000290"
     CDS             32263..32574
                     /locus_tag="KR432_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0638848.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            32622..32939
                     /locus_tag="KR432_000291"
     CDS             32622..32939
                     /locus_tag="KR432_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0638849.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            33152..33853
                     /locus_tag="KR432_000292"
     CDS             33152..33853
                     /locus_tag="KR432_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0638850.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            34055..35356
                     /locus_tag="KR432_000293"
     CDS             34055..35356
                     /locus_tag="KR432_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0638851.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            35493..36587
                     /locus_tag="KR432_000294"
     CDS             35493..36587
                     /locus_tag="KR432_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0638852.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            36589..37722
                     /locus_tag="KR432_000295"
     CDS             36589..37722
                     /locus_tag="KR432_000295"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBH0638853.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            37740..38849
                     /locus_tag="KR432_000296"
     CDS             37740..38849
                     /locus_tag="KR432_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBH0638854.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            38914..39654
                     /locus_tag="KR432_000297"
     CDS             38914..39654
                     /locus_tag="KR432_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBH0638855.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            39659..40651
                     /locus_tag="KR432_000298"
     CDS             39659..40651
                     /locus_tag="KR432_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH0638856.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(40838..41719)
                     /locus_tag="KR432_000299"
     CDS             complement(40838..41719)
                     /locus_tag="KR432_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0638857.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            41992..43380
                     /locus_tag="KR432_000300"
     CDS             41992..43380
                     /locus_tag="KR432_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638858.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            43408..44361
                     /locus_tag="KR432_000301"
     CDS             43408..44361
                     /locus_tag="KR432_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0638859.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            44421..44645
                     /locus_tag="KR432_000302"
     CDS             44421..44645
                     /locus_tag="KR432_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638860.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            44638..45150
                     /locus_tag="KR432_000303"
     CDS             44638..45150
                     /locus_tag="KR432_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH0638861.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            45182..46036
                     /locus_tag="KR432_000304"
     CDS             45182..46036
                     /locus_tag="KR432_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBH0638862.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            46603..48690
                     /locus_tag="KR432_000305"
     CDS             46603..48690
                     /locus_tag="KR432_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0638863.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            48705..48992
                     /locus_tag="KR432_000306"
     CDS             48705..48992
                     /locus_tag="KR432_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0638864.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            49019..50290
                     /locus_tag="KR432_000307"
     CDS             49019..50290
                     /locus_tag="KR432_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBH0638865.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            50348..50947
                     /locus_tag="KR432_000308"
     CDS             50348..50947
                     /locus_tag="KR432_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0638866.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            50952..51857
                     /gene="dapA"
                     /locus_tag="KR432_000309"
     CDS             50952..51857
                     /gene="dapA"
                     /locus_tag="KR432_000309"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0638867.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            51963..52595
                     /locus_tag="KR432_000310"
     CDS             51963..52595
                     /locus_tag="KR432_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBH0638868.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            53010..55181
                     /locus_tag="KR432_000311"
     CDS             53010..55181
                     /locus_tag="KR432_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBH0638869.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            55186..55803
                     /locus_tag="KR432_000312"
     CDS             55186..55803
                     /locus_tag="KR432_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0638870.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            55992..56336
                     /locus_tag="KR432_000313"
     CDS             55992..56336
                     /locus_tag="KR432_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBH0638871.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        22279 a         6135 c        10112 g        17984 t
ORIGIN      
        1 taactagagt attgtcagta atcttaaaag ttatacacaa aagtatccac aagctgtgaa
       61 taatttctaa taagttgata acatttatgc acattatcaa caagtatatt tataatatca
      121 aaatatatgt caaacttcaa tgatttgtaa ctaatttatc cacaatatta actaattgtt
      181 gataaaaata taaacatgtt aaaattactt tatttgtgta tataattgaa aaaaataatt
      241 tagaataaaa ttaaatagat ttttgctagt attttcaata aaaaaaatac ataatatata
      301 tatggtagat tttgtgatat aagcgtccca aaaactcgtt gacaacagta gtaaaagtat
      361 ttataattat atgtgttatg tttactttga gggtaaagtt attattttta taaacggaaa
      421 ataaaatctg aaaggcggtg caaaataatg agtaaaagaa cttatcaacc aaaaaagaga
      481 caaagaagta aagaacatgg tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg
      541 ttaaagagaa gaagagctaa aggaagaaat agattaactc attaattaca aaagggccgc
      601 tgcaaaggtg gcctttttgt gtaaataatt acaattcata gctgtgttta tggaggttaa
      661 ctatggactt taataggact aaagggttga aaaaagactc tgattttaga aaagtatata
      721 aacacggcaa atcttttgca aacaaatatt tagtaatata tatacttaaa aataaatcag
      781 attatagtag agtaggtatt tctgtttcaa aaaaagtagg aaaagctatt actaggaata
      841 gagtaagaag attaataaaa gaagcctaca gattaaatat tgatgaaaaa attaagcctg
      901 gatatgatat agtatttata gcaagggtat ctagtaagga tgctactttt aaagacatag
      961 acaagtctat aaaaaattta gttaaaagaa cggatatttc tatttaattg tatgctataa
     1021 atatggaggt taatgacttt gaataaaata atttatatat taaaggaaat aagtaggtat
     1081 ttatcgaatt tatgtattta tttagtgaga ttttatcaaa agtatatatc tcccttaaag
     1141 ggacctacct gtagatttta tccaacttgc tcacaatatt caatagaggc gtttaaaaaa
     1201 tatggatttg taaaaggtat gtatttaacg ataagaagac ttttaaaatg tcatcctttt
     1261 catccaggag gttacgaccc tctaaaataa aagagagtaa ctattaggag gtataaaaaa
     1321 attgaatata ataggaaatt tcttaggatt gattcttaag gtaatatttg aatttgttaa
     1381 tcattatggt atttctataa tactttttac gatacttgta aagattattt tattaccatt
     1441 aacaattaag cagactaagt caacaaaggc aatgcaagat atacaacctc gtataaaaga
     1501 gatacaagaa aaatataaaa ataagccaga aaaacaaaat gaagaaattg ttaagttata
     1561 tggagaagct aaaattaatc cactatctgg ttgtttacct ttattaatac agtttcctat
     1621 attaatagga ttatttagcg ttttgagaga accagttgct catggcgttt ttgcaaacaa
     1681 agctgcattt ttagcagctg acaatgggtt tttatggata aaaagcttaa catcaccaga
     1741 ttatgtatta gcagtatttt caggagcaag tgcatatgtc atgcaaaaag taatgacacc
     1801 caaagatcag ttacaaggct ctatgaaagt aatgacatat gtaatggcag gtatgtcttt
     1861 ctattgggga tttatattcc cagcaggact tacattgtac tggacagtaa gtaacttatt
     1921 ctcaatagct caatattact taataatgaa tcctttaaaa gctaaactgg ctgctaactc
     1981 taaggaggaa aaagttaatg agaaaaagcc taaaattaat aaaaagtaag agtaaagagg
     2041 aagctataag taaagctata gcagagttga atttaaaagc agaggatata gaagtagaaa
     2101 tacttgaaaa tccaagtaaa gggtttttag gttttatagg tgctaaagat ggtacttatg
     2161 aaatttttgt tatcgagagg gaagtagatg tagctaaaaa ttttatagag gtaatgttga
     2221 aaaatgcaaa tgttgatgcc aaagtcaatg tatctcaaaa agataattta atcatggttg
     2281 atattgaagg caaagaagct gcaagtttaa ttggtagaag aggggaaact ttagattcta
     2341 ttcaattttt gactggatta gctcttaata aaataaataa agattctcat acaagagtac
     2401 ttgtagatat agaaaattat agatcaaaac gagaagaatc tttaataaga tatgctaata
     2461 aagttgctag agaagtagcg aaaacaagaa aaactaaaaa attagattat atgaatccat
     2521 atgagagaag aataatacat tcagctcttc aaaatgacaa atatgtaatt acatatagtg
     2581 aaggaaccga tccatataga agattagtaa ttgagtgtaa aagataaagt caacctccta
     2641 aacattaggg ggttttattt tttaatatta ttaaatcatt aatgattggt taaatgtaaa
     2701 cctatatgta aaaaattatg aattaagagg tgataaattt gtttatagat gatacaattg
     2761 cagcaatagc tacagcacct ggagaaggtg gtataggtat acttagaata agtggagaaa
     2821 gagctttgaa ggttgcagaa gaaatcttta aatctatgtc gggaaagagt attgaagaat
     2881 acaataagag aacattaata tatggaaata tattggataa tgaaaatata attgatgaag
     2941 ttttattggc atatatgaaa ggaccaaact cctatacagg agaagatgtt atagaaatta
     3001 attgtcatgg tggatttatt tcggtaaaga agatattaga attaatacta tcaaaagatg
     3061 ttagattagc agaggctggt gagtttacta aaagagcatt cttaaatgga agaatagatt
     3121 tatctcaagc agaggctgta attgatgtta taaaagccaa aactgatata gctcatgaag
     3181 ttgctcaaaa tcaattagag ggttctttat caaagaagat aagagaattg agagataaag
     3241 tcactgagat attagctcat gtagaagttg ctatagatta tcctgaggaa gatattgagc
     3301 atataacata tcaaactctt aaagaaaaaa ctgatgaact taaaaaagag attaaaaaat
     3361 tatatgatac agctgaaagt ggaaaaatac ttagagaagg cttaaagacg gtaattgttg
     3421 ggaaaccaaa tgtaggtaaa tcttcactac ttaattctat tttaggagag aatagagcaa
     3481 tagttactga tataccagga actactagag atgttataga agagtttgta aacataaaag
     3541 gtataccttt aaaaattgtt gacacagcag gtataaggga tactgatgat atcgttgaaa
     3601 aaataggtgt tgaaaagtct aaagaatcat ttactagtgc agatttgata gtaatggttt
     3661 tagatgcatc tagaaagttg tcagaggaag atatagagat attagaaaaa cttaaggata
     3721 aacaaacaat agtgctgtta aataagaatg atttgaaaca agagattgaa gaagaaaaaa
     3781 tacttaaata tgtagaaaat aatagtataa taaaaatatc agctttacaa caagaaggaa
     3841 tagaagaatt gcaagataaa atagagtcta tggtatataa aggtagtatc aaaaataatt
     3901 ctagtttagt agtaacaaat tcaagacata aagatgcact tagtaaagca tataaatctg
     3961 caacagatgc tttaattgct ttggaacaaa gtatgccatt tgactttgta gaagtcgatt
     4021 taaaaaatat atgggactat cttggatata ttaatggtga tacagtaaca gaggatttac
     4081 tagataatat attccataat ttctgtattg gtaagtaatg tacaaaagta aatttgtttt
     4141 tattatagag atttttaaaa aatttgaagt gtttctcatg aaacattaat tttgaaaaga
     4201 aaggaattat aaatgataaa gtttgaagca ggaaaatatg atgttatagt agtcggagca
     4261 ggacatgctg gatgtgaagc tgctttggct actgcaagaa tgggatataa aactttgata
     4321 ataacaatgt ctttggattc catagcatta atgccttgta atccatcaat tggagggact
     4381 ggtaaaggtc aattagttaa agaaatagat gccttaggtg gtcagatggg attaaatata
     4441 gacaagacat atatacaaag tagaatgtta aatactgcca aaggtccagc ggtacactct
     4501 ttaagagcac aagcagataa atttaaatat catgaagaaa tgaaaaagac attagaagat
     4561 gaaccaaatc ttgatatagc tatggatgaa gttgtagaaa tacttcatga aggaaatgtt
     4621 gtaataggag ttggaactaa actaggatgt tcatttaaat ctaaagctgt aatattggcc
     4681 actggagttt atctgaactc aaagatatat atgggtgaag ttgcatttta tgaaggtcct
     4741 aatgcattgg ggtatgcaaa atatcttaca gatagcttag ttgagcttgg tcttagaatg
     4801 agaagattta agacgggaac tccagcaaga gttcatagag atagtataga cttctctgta
     4861 atgtctttac aagagggcga tgaaaaagta actccattct catttatgaa tgaaaatata
     4921 gaaaaaaaac aagaaccttg ttatttaaca agaactacag aagaaactca aaaagttata
     4981 ctggataatt taaaaaggtc tgctatgtat agtggagtaa tagaaagtac tggtccaaga
     5041 tactgtcctt ctatagaaga taaagtagta agatttagtg ataaaacctc acatcaatta
     5101 tttatagaac cagaagggtt aaatactaaa gaaatgtata tacaaggaat ttcaactagt
     5161 ttaccttttg aagttcaatt agatatgtac aaaactataa agggacttga aaattgtaag
     5221 ataatgagac ctgcatatgc tattgaatat gattgtgttg acccaactca actaaaaata
     5281 agtttggaaa taaagggtgt cgagaactta tttagtgcag ggcaatttaa tggaacttct
     5341 ggatatgagg aagcagcagc tcaagggctt atggctggta taaatgcagt tagaaagata
     5401 gaaggaaaag agccgtttgt attagaccgt tcagaagctt atataggtgt cttattagat
     5461 gaccttgtta ctaaaggaac taacgagcct tacagaatga tgacatcaag agcagaatat
     5521 cgactatatt taagacaaga taatgcagat atgagattaa ctcaaaaagg ttatgatata
     5581 ggattagtta aaaaagatag atatgaaagg ttcctaaata aaaaggctgc tgtagataaa
     5641 gaatttgaaa gattaaaaaa tgaaagagtt acaccaaaag aagtaaatag tttgttagaa
     5701 gaaaaaggag caacaccaat aaaggttgga atatctctat atgaattttt aaagagacca
     5761 gaggttactt acgaattgct tgaagaatta gggaagggcg ctggagaaga tgtttcaaga
     5821 gaagttaaag aacagtgtgt aataataact aaatatgaag gttatataga gaaacaattg
     5881 aaacaaatag accaatttaa aaaacttgag aataaaaagc tggatgagaa aataaattat
     5941 tcatctatag aaggtcttag attagaggct agacagaaat tagatgatat aaaacctatt
     6001 tcaatagggc aagcatctcg tatatcgggt gtttctccag ctgatatatc agttttactt
     6061 atatacctag aacaagtcag aagaactaga ggtggcaaag gtgaataata tagaacttct
     6121 gaaaaacggg attgaaggtt ttaatataga tacgaatgac agtatgttag aaaaatttaa
     6181 aatctataga gagatattag ttgaatggaa taaaaagatg aatcttactg gaatagaaga
     6241 tgaaaaagaa gtgtatataa aacatttttt agactctatt tcagctgtaa agaatggata
     6301 tataaaaaat gggatgtcta taatagatgt tggtacaggt gctggttttc ctggaatacc
     6361 attgaaaata tgtttagata gcttagaact tactttgtta gattcgttaa ataaaagaat
     6421 aaacttttta gaggaagtta gtagagtact agagttagat aatattacat ttatacatgg
     6481 aagagctgaa gattttggta aaaatgaaga ctatagagaa aaatatgatg ttgctacggc
     6541 aagagctgtt gctggattac cgattcttat ggaattttgt gtaccatttg taaaagttgg
     6601 agggtatttt atttgcttaa aaggtccgaa tgctaattta gagctagaag aatcacaaaa
     6661 ggctattgat gttttaggat tggagtatat tgaaaaaata gatgtagagt taccagaggt
     6721 agatttaaat cataatatat tagtttttaa aaaggtgatg gaaacaccag ttaagtaccc
     6781 tagaaaagct ggtagaccag ctaaaaatcc tattaagtaa tgaaaaaagt aatgaaaaaa
     6841 ttattattgg taagcttaat tatttcttag gaaataatta agcttaccgt aaaaaaacaa
     6901 aaataaagga attattaaag tagatgcaga aataaatttg tatgtacata tagagaacaa
     6961 aaataattta tatctaaaat aagagaggta tgttatggaa gataaaagag ttatggagat
     7021 acctatagaa gatgtagttc caaatccata tcaaccaaga aaaatatttt cacaagtttc
     7081 attggaggaa ttgagcaatt ctataaaggt atatggtata attcaaccta taacagttag
     7141 agcaaaagat ggcaaatatg aactaattgc aggagaaaga agattaagag cagctaaatt
     7201 agcagagctt aaaaccatac cagcaattat aaataatatg aatgatgaat cttctgctgt
     7261 tctagcgtta ttagaaaatt tgcaacgaga agacttaaat ttcattgaag aagcaattgg
     7321 ttatgaaaac ttaataaaag aacatgcttt tactcaacag caattagcag aaaaattggg
     7381 caaaaatcaa tctactgtag caaataagct tagaatatta aagcttccaa acgatataaa
     7441 aatgaagcta atagagaata atttaacaga aagacatgct agagcatttc ttaaactacc
     7501 aagtgaagat ttaatgcaaa gtgtgttaga caaagtcata aaaaatgaat tgactgttaa
     7561 aaaaacagaa aaattgatac aagatgtatt agaagaaact aaagttcaag aagagcctga
     7621 taaaaaacag aatataaagg gtgctatgag tataagaatt tacataaata ctataaagca
     7681 ggcctttgat gctatatcaa atacaggaat tgatgctaag tataatgaaa tagataaggg
     7741 agattatatg gaagttgttg tgaaaatccc taaaaaatag agattactgc ttaccagtaa
     7801 tctttttttg taatataagc cttgtaatat aaattgattt atagacatta tttatactta
     7861 aaaaacacct attcaacatt taaaattaat ttaattctta aaggggggaa catcatgggc
     7921 aaagttatag ctgtattcaa tcaaaagggt ggagtcggga aaactacaac aaatgtaaat
     7981 ttaagtgcta gcttaggaac tttgggaaag aaaatattag tattagattt agacccacaa
     8041 ggaaatacaa ctagtggata tggtattaat aaaaacgaag tagaaaatac aatctatgaa
     8101 attatgttag atgggttaca tattaaagaa gcaataataa gtactgaatt tgagaatgta
     8161 gatgtagttc catcagctac tgaactatca ggtgcagaga tagagcttac atctaaaact
     8221 aatagagagt atattttaaa aaattccata aaagcggtaa tagatgaata tgattatata
     8281 tttttagatt gtccaccctc actggggatg cttacaataa attgtttaac tgctgtagat
     8341 agtgttttaa ttccaataca atgtgaatat tatgctttag aaggtgttag tcaattaatg
     8401 gagactataa aattagtaaa atcaagatta aatgctgata tagaaattca aggtgttgtt
     8461 ttaagtatgt ttgatggaag agctaattta tcaatacaag tagttgaaga agttaagaaa
     8521 tactttaaag gtagtgtata tacaacttta attccaagaa atgtaagact tgcagaagct
     8581 ccaagtcatg gaaaacctgt aatttattat gataaaagat gtagagggtc tgtagcttat
     8641 ttggagctag cagaagaatt tatagattta gaagaggagg aatggtagta tggagaataa
     8701 gtctaaaaga agtaatagat taggtagagg tcttagtgca ttgataccag aaattaaagg
     8761 agaaacttca gaaaaagaaa tagtcaacat agatatagac aaaatatatc caaacgaagt
     8821 tcaacctaga aagcaatttg atgaagaaaa gataaaagta ttgtcggact ctataaaaaa
     8881 ttatggtgtt ttacagccaa tagtggttaa aatggatgaa aataataaat atatgataat
     8941 agctggagaa agaagattta gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat
     9001 aattaaagat atagatatga aagatataat ggagatagcc ttaattgaaa acttacagag
     9061 ggaagattta aattcaatag aagaggctct agcatataag agtttaatag agcattacaa
     9121 tgtaactcag gaagaaatat cagaagctgt tgggaagagt agacctcata taactaatac
     9181 actaagactt ttaaatcttg gacaagatgt tatagaaatg atagacagtg gtagaattac
     9241 agcaggccat ggtaaggctt tattgagaat tgcagataaa gatttgcaac ttcaaatagc
     9301 gaaaaagata gaagaagaag aactttctgt aagagaagtc gaaaatatag ctaaaaaaat
     9361 atctgaaaat aaacaagaag aacctaaaaa atctaaacct aaggatgtat ttattttaga
     9421 tgttgaagat aaacttagaa atatatttgg aacaaaggtg aatatttcta aaggaaagaa
     9481 gaaaggtaaa atagagatag aatactataa tgatgatgat ttaaatagta ttgtatcaat
     9541 gcttcttgaa tagaacaaaa atataaaaca gagaggaaga ttgttatgat ttatttagat
     9601 aatgctgcaa ctacatatcc aaaaccagaa agagtgtata atgctgtttt agattgtatg
     9661 aaaaattatt gtgcaaatcc aggtagagca ggccataaac ttgctatgag agctgctagg
     9721 gagatatatg atacaagaga gaatatagct aagttgttca atgtaagtaa tcctatgaat
     9781 atagtattta catctaatgc aacagattct ttaaatttag cgataaaagg tgtattacaa
     9841 gaaggagacc atgtaataac aacgtctatg gagcataatt cagtaataag acctataaaa
     9901 gcattggaaa aaaggggtat tgaaaatact gtagtaaaat gcgattatga gggattttta
     9961 gattatgaag atttagaaaa atcaataaag agtaatacta aactgattgt aacaacacac
    10021 gcatctaatg tatgtggaac tttaatagat ataaaaaaag ttggagaaat agctaaaaaa
    10081 cataatatac tatttttagt agatgcatca caaacagcag gtgtttatga tatagacgta
    10141 aatgaatgta atatagatat gttggcaatg ccaggtcata aatgcttatt tgggcctcaa
    10201 ggaacaggta tattatatgt aagagaaggg ttgaacttaa atatattaaa agaaggtgga
    10261 acaggtagta aatcagaaga aatagttcaa ccagagttat tcccagataa gtatgaatca
    10321 ggaactcata atactccagg tatagcaggt cttaaccaag gtatactatt tatatttgaa
    10381 agaggaatca acaatataag acaacatgaa gaagaattat gtcaatatat gatagacaaa
    10441 ctagaggaag tatcagatat aaagatatat ggaccaaagg atagtaaaaa aagagcatca
    10501 gtaatcgctc taaatatagg agatatggat tctggagagg ttactttctt attagatagt
    10561 gattataata tagcaactag atcaggaata cattgttcac cactagcaca taccacttta
    10621 ggtactttaa aacaaggtgc agtaaggttc agtataggat atttcaacac aaaagatgag
    10681 atagataaag cagtggaagc tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa
    10741 taatatagaa ataaaaaaag gggatatttc aaaataaaaa tttgaatcat ctcctttttt
    10801 gtacaaaaaa gaaaaggggc tcctaagaac ctctaatcta tttgataatt gtttcaagaa
    10861 attactttaa gtaatctcct tcttcaagaa ctatatgacc actttcgtaa ttagaaccat
    10921 caacagtaat ttttatttgt tcaacattca tattttttat gtaagtacga gcaactgaat
    10981 caagcattaa agtttctgca tcactaccta agtttgactt tgtaaagtca gagctaacat
    11041 ctaatatacc agtttttaca ccatcaatgt cttttgtagt aaatgaattt aattttgcag
    11101 attctggaac aactttaagt ttttgaagtt cttcaaataa agtgttctca tcaattttat
    11161 ttaaatcaac tttattttca attaaatctg tcttatctac atcaaaagag taaatagtag
    11221 ctgattgagt tttagtttct tcttttggag taggtttagc tttgttatta ggaacctctt
    11281 tttcagtatt gttttcttct tttggttgtt caacatttgt attgctctta gtttcttttt
    11341 tagatgtatc acttccattt tgagcattag tacaaccaac tgcgaatata gatatagata
    11401 atatacttaa cactaacatt attttcttat ttttcataaa aatttctcct ctcttaaaaa
    11461 ctattcttat atataattat agtcgttatg agcaaatata tgaatacaaa atagtaacaa
    11521 atttaaaaaa tgatttattt aaattaataa aactcaaaat aaactaaatt ttagtcattt
    11581 ttatgggaaa tcatttaatg tacagtgaaa atattgtaaa ataaaagtag tgtttttaaa
    11641 ataaatataa gttataatta tattataaaa aaatgtcttc aaaaggtggg aatagtatgg
    11701 aaattttgag cttaggagaa aagattaaaa aattaagaaa agaaaaaaac atgacattaa
    11761 aagaattagc tggagatagg ataacagcag cacaaataag ccatatagag agggataaat
    11821 ctcatactag ttatgaactg ttggaatatc taggcgataa gctagatgta agtgtagaat
    11881 acttattaga gaccaaggaa atgcaatcaa aaaagataac tgataattta atactacaga
    11941 gcgaaattta tataaaagaa aatgaattaa acaaggctga ggatcaaata aaagaagttt
    12001 tagatatatg taagagatac gatttaatgg agaactatgg cagatgtaat tttttaatgg
    12061 ctaatattaa tttgaaaaaa tctaattata atgatgctat agaaaacttt gaaaaggcat
    12121 tgtacttctt tataaaaaat agtgatagtg aaaatatact taagtgttat ttaaacatag
    12181 ggaaaatata tatgcaggaa gacttttata aaggtgctat aagccacttt aattttgcag
    12241 aggaaatttt gtcagagaat aaatttgagg atgttaacat acacaaggaa ctatatagta
    12301 agatggcata ttgttacata aaactagata atccaaatat gtcattgaag tacatagaaa
    12361 agataaatga aatagataat agaaatgata gaaaagaaga tgtcgatatt ttggtgttaa
    12421 aagcaaataa tatgcttgag attggaaaat atgaagaatc aagagattac tttaaaaaag
    12481 cactaaaaat acttgagagt gaggataata aaactggatt agcaaatgtg tatttaacaa
    12541 tttgtgatgt ttacagaaaa ataggtgaaa cagatagagt acttgaatat tcacaaaagg
    12601 tatatgacat aaagaaaaat gatgaagatg aatacatgat gtcaggatta tttaagataa
    12661 tagaagcata tatagataaa gaagattatg atttagctag aaaatattgt aagatagcct
    12721 tagcttcatc aataaaaaat aaaaataaat tcaatgaata taaagcattg agattctatg
    12781 ctaatatgta taaaaatcag aatgaagtaa ctcttgcaat tgaatatctg attaaatgca
    12841 taaaaatagt atcagattta ggcaacaata gaatattggc caatttatat atagacttag
    12901 gtcaattata ttcaaatata tcaaaagaaa aagagttaga atattatcaa aaaggtgtat
    12961 ttatgtacaa aaatttagaa ataatgtagg gtaaataaac ctataagggt atagaaacaa
    13021 tctataacaa gcaagataat atctatgata aaatataaat tgtatgaata tttttaagga
    13081 gatgaatcag acaatgagta taaaaataga tgcgagaggg cttgcttgtc ctaaaccagt
    13141 tataaataca aaaagagaat tggataacct agaagaaggt gttgtagtta caatagttga
    13201 taatgaaaca gctaaggaga atatattaaa acttgctaaa tctttaagtt gtgaagcaaa
    13261 tattgttgac gaaaaagaga attttatatc tatagaaatt agaaaaggta aaaatatact
    13321 agataaacaa aatgttgtag ataataaaga aagtgaatta gacaatacat gtatatttat
    13381 atcttcagat aagatgggac ttggaaatga cgaattagga aaagtgttaa taaaaggatt
    13441 tatatataca ttaacagaat ctaaacctta tcctaagtat gtacttttag ttaatggagg
    13501 agtaaaatta agtgctgaaa atgaagaaac catagaaaat ctaaaaatac ttgagaatat
    13561 gggtgtagaa gttttatctt gtggtacttg tttagattat tataatttaa aagacaaatt
    13621 acaagtaggt acagttacaa atatgtatac tatagttgag acattaaaga atgcatcaaa
    13681 taccatttct atataatgta tgtttgttta ataatataaa actaataata aagattaact
    13741 caaatagata gtcagaaagg attaagtaat gaatcaaatg tatatagtgt catttaattc
    13801 gacacatcat gcgataagat cagaaaagtt atttggtgaa aatagtctaa aagtaatggc
    13861 tcttccaaca ccaagagaaa taacagcaag ttgtggaata tcaattaaat tttcttttga
    13921 agatatggaa aagataaaaa ctattttggt tgaaaataat gtagatataa aaggaattta
    13981 ttgtataagt aaattagaaa atggttcaaa agaagtagag aagttagatt aggaggctaa
    14041 ttatttatgc caatggattt aaaaataggt gatattgttg aattaaaaaa gcaacatgca
    14101 tgtgggtgta aagagtttga aatcataaga acgggaatgg atataaagat aaaatgtact
    14161 aaatgttcaa ggctaataat gcttgataga gagaccttag aaaagagagt aaaaaagcta
    14221 ataaaaaagt gataaaaatt taacacttaa aaaatgtcaa ataccgtata ttaatatcca
    14281 aaattttggt ataataagaa ttgaagaaaa cattttcatg ggggtgcttt aattatggca
    14341 gttaaatatg caaaaagaat gcaaggatta caaggatcag aaatacgtga gcttttaaaa
    14401 cttactcaac aaccacaaat aatatctttt gctggtggaa tgccggctcc agagttattt
    14461 ccagttgagg aaatgaaaaa agtatcagtt gcagtgcttg aagaaaatgg aagatcagct
    14521 atgcaatata ctacaacaga aggatatgaa ccattaagag aaaaaatagc agctagaatg
    14581 aatgataaaa acaaaactaa tgtaaacaaa gatgatatat tagttacaag tggttctcaa
    14641 caaggtctag attttgcagg aaaagtattt attgatgagg gtgatgtaat tttatgtgaa
    14701 agtccatctt acataggagc tataaacgca tttaaatctt atcaacctaa attcatagat
    14761 gttccaacag attctgatgg aatgataatg gaagaattag aaaagatact agaaacaact
    14821 gatagaataa aaatgattta cgtaatacct gacttccaaa atccaacagg aagaacttgg
    14881 ccacttgaaa gacgtaaaaa attcatggaa atagtaaata aatttgaaat accagtaata
    14941 gaagataatc catatggaga tttaagattt gaaggagaga ctttaccatc tttaaaatca
    15001 atggatacaa aaggattagt aatattctta ggaactttct ctaaaatatt ctgcccagga
    15061 tacagattag gatggacttg tgcttctcca gaaattttat ctaaatttaa ctttgcaaaa
    15121 caaggtgcag acttacaagc atcaactata tctcaaatgg aagtaagcaa atttatggat
    15181 atgtatgacc tagatgcaca tgttgacaaa ataaaagcag tttatgttaa acgtagagat
    15241 gtaatgctta agactatgga agaagagttc ccagaaggat tagtatttac tcatccagaa
    15301 ggtggattat ttacatgggt agagcttcca agtaatttaa atgctaaaga attaatgcca
    15361 aaatgtttag agaagaatgt tgcttatgtt gctggaggag gattcttccc taatggtggt
    15421 agagaaaata ctttcagact taactattca aatatgccag aagaaaaaat aatagaaggt
    15481 ataaaaaata tagcagctgt attaaaagaa gctatgggtg tagaagctta attaaaaata
    15541 ataacttata tataatacaa gggatggttg ttgccatccc ttgtttgttt gaaaagtata
    15601 taattcacct tatactaaac tttgtccaaa atttaaattg acagaaataa gaataaatga
    15661 taaaatgtgt aggtatgaga ttttagtctc atttctctgc tctaagagtg tagagccgtt
    15721 aagtccaaag ggaggtgaat tatagtgaga aattatgaat tagtttatgt ggtaaagcca
    15781 aattctgatg aagaagtaag agaggctata ctaaacaaag ttaaggaagt tgttgcaact
    15841 gacggagaaa tagttaaagt tgatacttgg ggaactaaaa aattagctta tccaatagct
    15901 aagtttacag aaggtttcta tgtattagtt aacttcaaat cagcagtaga cgttcctaaa
    15961 gagatagaca gaaacttaaa gataaatgaa aacgtaataa gacacatgat agttgttgct
    16021 taataactgg ttttattata aaaaataggt ggtgttttta tgaatcaagt tgtattagtg
    16081 ggaaggttga ctagagaccc tgaattaaga tacatcccag gtacaggaac accagtggca
    16141 tcctttacaa tagccattga tagagactat gtaaaaaaag atggatctaa agaaactgat
    16201 tttatacctg ttgaagtaat ggggaaatct gctgaatttt gtgctaacta cataactaaa
    16261 ggtagattag tagcacttca aggatctata agagtagaca attatcagac acaatcaggt
    16321 gaaaagagaa cttttacaaa agtaagtact agatcagtac aagctttgga tagtaaaaat
    16381 aaatcggaaa attcatatag agagagtgct ccagcttttg agccaagctt tgaacctcaa
    16441 ggtttagacc cacaaggttt tcaagctata gatgatgacg atataccatt ttaaaaagga
    16501 gggaaaaaag tcatgatgaa taagaaaaga cgtaagaaaa aaagagtttg tcaattttgt
    16561 gctgacaaaa acgctaaaat agattacaaa agtactcaaa gattacaaaa gtatataact
    16621 gaaagaggaa agatattacc aagaagaata tctggaactt gtgctaagca tcaaagagaa
    16681 ttaacagttg ctataaaaag agcgagaaat atagcacttt taccatatac attagactaa
    16741 aaaaatgagc cttgtgctca ttttttatta tatgtattta tatatctaaa tgaggcgagc
    16801 tataaagctc gtttttttgt ttatatactt aaatatatta ttatttatga tataatatta
    16861 tatattaagg agggttgtaa acgtgaaaat agataaaggt tctgagataa cgagaaacat
    16921 aaagataata gaatggatga aaacagaaat attaatgagt gtaagtgatt tgtttaattt
    16981 attgtttaaa ggggtaaaac cattagatga agcaattcaa gatactttag caaatataat
    17041 aatgataaca tatctcttag caaagagatt aggaattagt tttagagatg tggattataa
    17101 agtcaaggaa aaaataaaaa ttggtataga tgaggaccat agcgttgaaa gatggtatgg
    17161 agatttatct aatttaaaaa aacatatgga taatagggag tgatattaaa ttgaataata
    17221 aaattagact atctaaggca atgtctataa tagttttagc tataataatc gctttagtaa
    17281 tagcctatat tccaatgcta ggaatattta gtatgttagc agctgttcca tatgtaatta
    17341 taggtgctat tactgataga aagtattctc ttatatctat tttggttaca ttttgtgtac
    17401 tgattctatt tgcagacatt tcttatgcat taaatatatg tataatgtat tctttaccag
    17461 gtatagtaat aggaaagatg ctaaagagaa gctttgaaca agaggacagt aataaattcg
    17521 aaccgatata tggtggaacc ataatatttg tactttcaat gcttgtatat ttttttgtat
    17581 taaagacatt tttaaatgtg aatctattag atgaagtttc taagatgata tcagaaattg
    17641 taaatattca aaaaaatagt ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc
    17701 cagatgagat tgtaagttat tttacaaata tgctaccaat gatgttattt ttacaagggt
    17761 tgttatcagc gtttgtcaca tattatttga gtgtattttt tataaaaagg attacaaaga
    17821 tgaatataag atttcctaaa tttgctgatt tttatttacc aggaaatgct atactcacaa
    17881 ctttcttact ttatttatta gtcttattta ttcaaattat tggttcaaaa ttatatacag
    17941 aattaataat gactaattta cagctagttt ttaatttaat gtttgtaatt caaggtatag
    18001 cagtttgcat ttatttctta aagaaatgga taagacaggg accaaataaa attatgtttt
    18061 tttcagggat aattttatgt ttatttggat ttatgggaat atcttttgtt ggtatggtag
    18121 atagtataat tgactttaga aaggtaagaa gctataaatc cacttaggag gatagaaatg
    18181 agtaacaaac aaacctttaa attaaatatg ccagaaataa atttatatat aattgttatt
    18241 ggtatttcta gtataatttt gctttattac aacttgtatg tagggtgttt atttttctgt
    18301 atttttgtat atatggtttt tcacaattgg agaactacta atattagacg tcatgaatgg
    18361 acagagtata ttcaaaattt atctttagat atagatgaaa caactaaaaa agccataata
    18421 aatcttccaa taccattatg tattttagaa tttgatggaa atatatcctg gtataatgga
    18481 aagttttatg atatgattgg tcaaaaagat ttacttgata aaaatataga ggatatagtt
    18541 aaaaatctta atttaagaaa agtattaaat gaaaataaag agatgtacac agaaataaac
    18601 tataaagaaa aagaatatac aataatttat aatgttataa aaaatgatca agaaaaaaat
    18661 cctaaatatt tgatgatatt atactggata gacaagactg aatatttaaa agtaaaacaa
    18721 aattatgatg atgaaaaaaa tgcaatgatg ctaatacaag tagatggata tgatgaagta
    18781 ttaaaaagtg cagcagaaga taagagagct ttaattaatg ttgaagtaga gaaaatactt
    18841 tctgctttag aattaaattc aaatggtgca ttgagaagaa cttcaaaaga caaattcttc
    18901 ttggtaatgc ataaaaaaga gctaaaaaaa ttggaagctg agaagttttc tattttagat
    18961 acaataagac atattgatta tggaaataat cttcctgtta ctataagtat aggaattggt
    19021 atagatggag atacattaaa tgagaaccta aaacttgcta cgggtgctct cgatttagca
    19081 cttggtagag gtggtgatca ggctgttgta aaaacaaaag ataaatttgt gttttatgga
    19141 ggaaaatcta aagctgtaga aaagaaaacc aaagtaaaat ccagattaat aggtcatgct
    19201 ttaagagagg ttatacaaca aagtgaccaa gtttatataa tggggcataa atatcctgat
    19261 atggatgcta tgggagcagc agttggtgtt tatgatatat gtaaatcctg taacaaaact
    19321 gcaaacatag ttttacaatc tgtaaatgaa tctatagaaa tatttataaa taaaataaat
    19381 gaaaataatt actataaaaa gctgtttatt ggaaaagagg aagctattga caactgtact
    19441 aaaaatacat tagttgtagt agttgatact catagaccaa attatactga atgtgaagaa
    19501 ctgttaaagc tatcagagaa ggtagtagtt atagaccatc atagaagagg tgtggagttt
    19561 ataaatgatg cagtgctttt attccatgag atatatgtat cctcaacgtg tgaaatggtt
    19621 acagagttag ttcagtatat ggatgaagat gtaacaataa ataaacttac agctgaaggg
    19681 cttttagcag gtataagtct agatactaaa aactttgcat tcaaaacagg tgttaggact
    19741 tttgaagctg cttcttacct tagaaaagtt ggagcagata ctatagaggt aaagaaattc
    19801 ttcaattctg atgtaaaaga tttcataata aaagctgaaa taattcaaag tactaaaata
    19861 attaataata gaatatgttt agcatattca agtactgaga tagatagtat aaatgttata
    19921 atagctcaaa ctgctgatga gttattaaat atcaaagaag tagaagcttc ctttgtatta
    19981 ggtgaaaaag atgatactat atttataagt gcgaggtctt tagggcaaat aaatgtacat
    20041 gtacttatgg aaaaattagg aggcggggga cacatagata tagctggagc acagttaaaa
    20101 aatgtttctc taaaagaagc ttataagatg gtaaataaaa taatagagga gtatttggag
    20161 gaggaagaat agatgaaagt tatattatta aaagacgtaa aaggaactgg taaaaaggga
    20221 gaaatgaaag aagtaagtga tggatatgca agaaatttct tatttcctaa aaagatggct
    20281 gtgcaagcag atagtgtagc aataaaagaa ttaaatgaaa agaataaatc taaagaaata
    20341 aaagcacaaa aagagtatga agaagcagtt ttattaggta agcaaatgga agaaattaat
    20401 atagaaatat attcaaaagc aggagaaggt ggaagattat ttggttcaat aacttctaaa
    20461 gaaattgctg agcaattaaa gaaacaaaaa gacatagacg tagataaaag aaaaatatta
    20521 ttagatgaac caataagatc tttaggttca actttcgtag aaataaaaat acatcaaaaa
    20581 gtaactacta aaataagagt ggatgtaaaa gaaaaacagt aataactaga ggtgattaag
    20641 atggaagata tgacgagaat tcctcctcat agtgtagaat cagaacaatc aatattgggt
    20701 tctatacttc tagataaaga tgctataata acggttacag aaactataaa gcctgatgat
    20761 ttctataaag aggctcacaa aataatatat gaatgtatga taactttaag taataaaggt
    20821 gagcctatag acttgataac attaacagaa gaactaagaa aacaagggca tcttaatgat
    20881 attggaggaa tcagttatat tacaagtctt tctactatag taccgacgac ttcaaatgta
    20941 aaatactatg ctgatatagt aaaagaaaag tctgtactta gaaagcttat aaaagcttct
    21001 aatgaaataa taaatctagg atatagtgga gcaactaaga ttgaagatgt tttagaacaa
    21061 gctgaaaaga gtatatttga tatatcacag gagaaaacta gtgatgattt taaatctata
    21121 aacttagttt tgatggatgc ctatgatatg atagaaaaat tatatactaa caagagtgat
    21181 gtcactggta taactactgg ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg
    21241 actgatttaa tattaattgc agctagacca gcaatgggta aaactgcctt ttctttaaac
    21301 ttggttcaaa atgcagcctt aaaaggtgat gcctctgtag ctgtatttag cttagagatg
    21361 tctaaggaac aattagtaca acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa
    21421 ataaagactg gaacacttaa tgataatgat tggcctagaa ttatagatgc tatggcagta
    21481 ttatcagatg ccaaaataca catagatgat actcctggta taaaaatatc agaactaagg
    21541 tcaaagtgta gaaagttaaa aatagaaaaa ggactagatt tagtgcttat tgattatctt
    21601 caacttatgg aaggtgaagg taataatgaa agcagacaac aagagatatc taagatatca
    21661 aggtcactaa aaatattagc aaaagaatta aactgtcctg tagttgcact atctcagtta
    21721 tcacgtgcac cagaacaaag agcagaccat agacctatgt tatctgattt aagagagtct
    21781 ggagcaatag aacaggatgc agatatagtt atgttcttat acagggacga atattatcat
    21841 gctgattctg agagtaaaaa tataggagaa gtaataatag caaaaaatag acacggtgaa
    21901 actggttctg tggaacttgt ttggcttggt gaagttcaaa gatttgggga taagttgaga
    21961 gacctataat ctaacgaata tccacagaac gtcctttctt tctacaggaa aaatgtggat
    22021 acatgtcact aaatatgtcc aaacattgaa atttagctaa aaaactatcc acactatcca
    22081 cagatagcct gttgataagt gtgaataagt agataaagca agcttttggc ttgctttatc
    22141 tgtgtataaa tattaattat taggaggaat aatatggaaa aaataagctt aaaatatata
    22201 tatcccaata taattaaagt acttgatgaa ataaacttat ttagagtaat tgacaataat
    22261 ttaagagagt ctattgttgt ttatgctaat aatgtggata atcagtatca tataaacatg
    22321 actaatacaa actttggaaa cataattaac atatgtaaat tagaaaaact tttagatgtg
    22381 gataaattta tggaaaaagt tataaaatat gaaaaagaaa taatagagaa agaagagttt
    22441 tcaaaaatag aagaatatat gttaaatatt ggagaatact aaaaaagttg aaaaaaatac
    22501 gaatgtttga tagaataagt aagtaaagtg ttcgagagta tataaaatat agaggtgaat
    22561 tacatgaaaa cagttgcaat tgttggttct caatggggag atgaaggtaa aggtaaagtt
    22621 atagattatc ttgctactca agcagatgta gtagtaagag ggcaaggtgg aaataatgca
    22681 ggacatacat tagttgtaga aggtaaaaaa tacgcattac atctaattcc atctggagtt
    22741 ttaaatccaa atacagtgaa tataatcgga aatggaatag tgtttgaccc taaaggattt
    22801 ttagaagaat tggaaatgtt taaaacggat aatataagta cagaaaatat aaaaataagt
    22861 gatagagcac atgtcatatt cccatatcat aaagaactag atgcattgtc agaagaagcc
    22921 agaggcgatt taaagatagg tacaacaaaa aaaggtattg gaccttgtta tatggataaa
    22981 acagagagat ctggtattag aatatgtgac ttaatggata aagataaatt tgctataaaa
    23041 ttaaaagctc aaatagatgc taaaaataaa atagttaaaa atatatatgg aaaagaagaa
    23101 ttatttgatt ttgaaactat atataacgaa tatctaggat atgcagaaca aataagaaaa
    23161 tatgtggcag atacgtctgt tattgtatat gatgcagtaa gagcaggtaa aaaagtattg
    23221 tttgaaggag cacaagggac tttattagat ttagatttag gaacttatcc atttgttaca
    23281 tcttctcatc caacatctgg tggatttgca attggtgcag gtataggacc aaatatgata
    23341 aaagacgttg ttggtatagt aaaagcatat actacaagag ttggagaagg accgtttgtt
    23401 acagagcaaa taaatgaaac tggagataag ataagagaac aaggacatga atttggggta
    23461 actactggta gaccaagaag atgtgggtgg tttgatgcag ttatagttaa gtatgcagct
    23521 agagttaatg gattgactag tatttcattt atgttacttg atgtattaac tggatttgat
    23581 aagataaaag tttgtacgtc ttataaaatg ggcgataaga taataacgga tttcccagct
    23641 tcattagatg atttagcaaa atgtgaacct gtatatgaag aattagatgg atggaatgaa
    23701 gatataactc aaatagataa ctttgatgac cttccagaaa atgcaaagaa atatgtagca
    23761 aaaatagaag aactagttgg agtaagtgta gatatggttt ctgttggtcc aaatagagct
    23821 caaactataa taagaagaaa tatatttgct taatatttta tcaattaaat aatttaaaat
    23881 agttgaagaa gagtatctca agataatgtt tatagttatt agagatgctc ttttatattt
    23941 gacttacatt gattttttat taagttaaag ctacttatac atattctagc tgaaattaaa
    24001 gttgaattta tgtagataca tataggtttt ttaaaaagaa ttttgttgaa aataaatggc
    24061 taaagatgat tttaattata ttctaaaaaa agtaaaaaaa actattgact aaaaattgta
    24121 tagacgctat aatataactt gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg
    24181 tctattagct cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg
    24241 cggatagatc accaaaatat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
    24301 cggtaacagg ggttcaattc ccctacgagt caccagtatg ggactatagc tcagctggga
    24361 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttagtcc caccaaattt
    24421 tgaattagag ataaatacta gattatatgc tggtgtggct caacggtaga gcagctgact
    24481 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccacaaagta cataaattca
    24541 aattaatata attttctatg ttacaggata cttttaagta tcttttttta atttcatttt
    24601 tattgtatta aaaatgaaat taaacataca acaaaatttt gaattacttg caatgtgata
    24661 gttttaaata taaaatttag aatcattcta gcatagtatt atgtaatata gcaaatttaa
    24721 tttatagtat caataaatat agactactaa gaatgtaaat gatttgttag attaagatac
    24781 attagatatg taaatagaaa aataaaaaat atgttttaat ttatgtggca taagttttaa
    24841 acagagaagc aagtttgctc ttacctctct atttaaaaat aaaaaaatgt tttttaaggg
    24901 ctattttatt taaatctttc ccatctcaaa tcatctccag taaattctaa aatcctaaac
    24961 ttatctaaga ttctagagag agttctttga gtgtaagtat ttcctatttg agatggtgtt
    25021 aagttggttg aaataatgat ttttttacca gcaacaagtc ttgtatttac aatattgaag
    25081 atttccccac tagtaaaaga attattaagt tcagttccaa ggtcatctat tattaataaa
    25141 tcacaatcaa acaagttttt ataattatct tcacttattt ggttatttgt gtctcttcta
    25201 aatttataat cttcaagaat atctaaaatt ctaaaagaag tttgatagat tacaacattt
    25261 cctttatcta acaattcctt tgcaatacaa ttgcacatat aggtctttcc tagtccagta
    25321 gaaccataaa acagtagatt ttcactatta tccttcttaa aatcatgaac aaaattttcg
    25381 cagatactta aattatttag catattttct ctaggagata tttccccatc agagcctttc
    25441 ttaggagaaa atatattcaa gttaaaattc gaaaaatttt cctgacttaa aatcctatct
    25501 aaattagaca ttttataagc ttcatttaca atttcttgtt tgaggcaaga acatttttct
    25561 ccattaggta aaaatccttt atctttacaa gaaatacatt gaaactttat ttccaagtaa
    25621 tcaagaggta tgttactttc agataaaagt ttttctttct ttacttttaa agactctata
    25681 ttctctttag tcttctttac aatttcatct ttagatttgg gatttaaaag tactatctta
    25741 gctaaactga gacctatttt agaaatttca tcatctatag attttatctc agggattcta
    25801 ttatatacct cattttttct atgttctaat aagagttcat tatcatctct tctcttagca
    25861 tatttagcaa gtatttttct tattttatcc tcattcattt aatcacctac ttaaactttt
    25921 ctttttgact ttttttaata atctcatcga gctcatcaga ggtatactga gtgaatgttt
    25981 cgttgaaatt atgaaactta gtttttttgt aagtattgtt gttttgagta gtattaatct
    26041 gcttcttatt aatattctcc ttaactatac gttcttcctc tttttgcttt aaatcattta
    26101 aattttttat atttttttca ttccaatttt taattatacc atttatataa gatatacttg
    26161 ggtttgaaat attcttagac ttagaacatg catttattat caaatctata tccatattaa
    26221 atttatcaaa ccaggtgtcc ataagttctt tttctgattt tgatggttgt cttgaaaatc
    26281 ccaattcatt gaaaatcgtt ttatataaaa tatatcgttc acttcttacc aaaaagcttt
    26341 cttcaacatc ttttggcgta tataaatttg aatcatacca atttctaata atgccttcaa
    26401 tgtatttaac tggctttgat gtaccagttt tatctttgac gtattcatat gcgcaaagta
    26461 tcatatcggg gctcatattg tatttattca ttatatccat aatactcatc ttttcacttg
    26521 ggtcgagata tcgcccaact attttattta tagaattaaa catttttcta atacttggat
    26581 tttcagaagt agatacaacc ttatctgtat tagattttat agatgaatta ttgcttaata
    26641 tattctcaat atagaaattt tttaaatcta aaaattctat tgaataatta aaattatcat
    26701 attctccatt gtcatgtatt tttataatct tcttttcttc ccagaatttc caagcagata
    26761 acacatcaga caatggtata tttaggtttt tggctattga attattgtcg aacttaggat
    26821 tagaagtaat atcacatgct tgtctatatc ctaaaagata aacttttacg tataacccgt
    26881 ccgccattgg cataaatata tctataaaga tattgggaat agtgatttca cctaaatcta
    26941 tttcattgct ttctttaaaa aacattttat cacctcagat taaattatat cattttttat
    27001 attatatttg tctgtaaaca aataaaaggt cacaagatta gcatgtaaat attttctaaa
    27061 agtattcatt ttgaaaagga ggagatattt tggttataaa attcaaccaa aaagtaagaa
    27121 gaacattatc agtagcgatt ctatttatgg tattaataat aggttgcttt atagaaaagg
    27181 atgacagttt agaaacatac aaagaaaaaa ctaattcaaa aataaatcag tataatatgg
    27241 atgttatatt tgatgatgag actaagaggt taatgtgcaa tcagaatgtt gactacataa
    27301 ataatacaaa atcaaacata gataaaatat atttccatat atatccgaat gctttttcca
    27361 agaaagattt tgctcctttt gaaaaaagtg aaatgacaag ggcttatcct aatggattta
    27421 acgaaggata tatagatata aagaatatat taaataataa tagtaaaatg gagtataaaa
    27481 ttaaaggaga caaaaatgac atattagaaa tagaattagg gaaagagtta aaaccaaatg
    27541 aaagaatgtc attagatata aaatataacg taaaaatacc aaattcaatt ggaagatttg
    27601 gatatggaga aaatactata aatgttacta attggtttcc tatagcttgt gtaaatgatg
    27661 aaaggggatg gaatttaaag agttatgaaa ctattggcga cccattttat agtgagacta
    27721 gcgattttca tgtgaaacta ttgattccaa ataaatataa aattgctcat acaggaaaac
    27781 ttattgatga taaacatgat aataagaaaa tgctatatga aatagaagct gaaaacgtta
    27841 gagattttgc atttatacta agtagtaaat ttgatgtaaa caaagtagat tcaaagggag
    27901 tagctatcaa tacatataat ttaaataaaa agctttctaa aaaagcaact gaagtggcaa
    27961 aagattcaat aaatatattt agtgaattat ttggaaagta tccttacaaa gaattttctg
    28021 ttgtagcaag tgactttttt atagggggga tggaataccc tatgcttgtt atgatagacc
    28081 aaagtttata taatgaaaaa aatgaatttt tactagaata tgttattgcc catgagacag
    28141 cacatcaatg gtggtattct gctgttggaa atgatgaaat aagtgaaccc tggttagatg
    28201 aagcacttac tgaatattct acaatagttt attttgaaca aaagtatggt aaagatatgg
    28261 caaacaagct tataaaaact atggagatac agacaaaaag ttatttgagt gaaaatatat
    28321 ttaagccagc aaatgagtat aaaaattcta ctgaatacag cttgaatgtt tatacaaaag
    28381 gcgcaatagc atttaatgaa ataagaaaag aagttggaga taaagtattc tttgagactt
    28441 taaatgaata ctataataaa tataagcata aaaatgctaa tggaagagct tttgtggaac
    28501 tatggaatag taaaggtgta gatataaata agattataag tgaatgtaag taaatagtga
    28561 atgtaagtaa attttgtgca gagtattaaa ttttatttaa tactctgttt ttttggtata
    28621 atataattta taaggcagaa aaagaggtga aggaatgcta aagtattgtt cagtagggag
    28681 tggtagtagt gggaattgcc actatatagg gtataaaaat actaatatac tagtagatgc
    28741 aggattaagt ggcaagagaa taactacagg tcttaaagac attggtgtag atgcagataa
    28801 attaaaagga atatttataa cacatgagca tgttgaccat ataaaaggag caggtatatt
    28861 atccagaaag ttcgatatac cagtatttgc taatataaaa acatggtgtt ctatgaagga
    28921 caaattaggt gacatcaaag ataaaaatat gaaagtattt gaaaatgata aaacctactc
    28981 attaggtgat atcatagtga gacctttttc aatagaacat gatgcgtctg atccagttgg
    29041 atataatttt tatacagaga gtgatgaaaa aatgtctata gctactgata taggatgtat
    29101 aactcaaaat ataaaaaaac atctatataa atctaaatta gtagtattgg aatcaaatta
    29161 tgaccctaat atgcttatga tgggttcata tacatatgct ttaaagaaaa gagtaatgtc
    29221 aagtactggt catttatcca atgaagatgc agctaatttc tgcgttgaac ttattaatga
    29281 aggtacagaa tctatattat tagcacattt aagtaaagaa aataattttc ctgagttagc
    29341 atatgagact tcaaaaggag tattagcatc aaatgatata gtagttggac aagatgtaaa
    29401 actagatgta ctatcacgaa atgatgtttc taatgtttat gagatgaagt agaggtgaat
    29461 aactttgcta aagagaaatt ttgaaaatgt tgaggataat aaaattttat actctataaa
    29521 gcagggtatg attttagcaa tacctgctat tatgactggt tcaacggctc ttgtaatttt
    29581 gaatcttcca ataaaggctt atcaagaata tttatcaggc ttgtttaatg gagaggttac
    29641 aaatattctt aactttataa atgattctac tttaggaatt atttcattaa taatattatt
    29701 aacaataagc tatagttatg gaaaaatata tgggagcaag tatacagtac ttgttcctat
    29761 agttgcaatg tgctcttttt tagtattttc acatggtaat gagcttaatt cttatataga
    29821 aatatttaag acaaagtggt tatttacatc tataattgtt tctatgacat cttctgtatt
    29881 gtttgtaaaa cttacagaat cttttgttac aagtgtaaag tttcatacag aaggggctga
    29941 tgcagacttt aatatggtag tttcggctat agttcctttc ataatagtag tatttttgtt
    30001 ttctattttt agagtgataa tgataagtct aattggcagt tcaaactttc aagatatatt
    30061 ttataattta ttttcaaatg tttttaataa aatgggaaca aaccttatga gtgctctgtt
    30121 gtttattttt ttaatgcact ttatgtggtt ttttgggatt catggtagta atgtattaga
    30181 taccgtcgca aaaaatttat ttgaaaatag tatggcaatt aacataaatt tagttaataa
    30241 taatcaatta ccaacagaaa tttttactaa gacctttttt gatacaatgg ttttattggg
    30301 aggatgtggc tctttactat gtttagtaat tgcaattttt cttagtgaaa aacgtataaa
    30361 tgtaagaaag ctagctaaaa ttgcttcaat accagctctt tttaatatca atgagatgct
    30421 agttttcggg ataccaattg tttttaacta tattatgttt gtaccttttg taattactcc
    30481 tataatctta accattacaa gttatgtggc tatgtctaca ggtattgtac catgtacagt
    30541 ttcagctgta gaatggacct cacctatatt tttaagtgga tatatgtcta caggttcaat
    30601 aagaggtagt ttgttgcaag tatttaattt atgtgttggt gttatgattt atattccgtt
    30661 tataaaaatg tcgcaaaata gatatacata tatgtttaag aataatatag aaaatttaac
    30721 aaacttatta aaaaggtatg aattaacagg tgaacagcct aatcttctaa accataatgg
    30781 taaaataggt tcaatttcaa aaatgttggc atcagacttg aagtttgcta tgaaaagagg
    30841 agaaatagaa cttttttatc aaccacaagt caattataat ggaaatgttg tgggagcaga
    30901 gggattactt agatggaaac attcaatagg agggttcatt tatccaccac tagttataat
    30961 tttggctaaa gaagaagggt tcttagatga gcttggagag tatattatta ataaagcatg
    31021 tattgattta aagaagagtg aaaagctatt gagaaatcct gttaagttct ctgtaaatat
    31081 atctccagac caacttgatg accctaagtt accggagcaa ataaaaaata tcattagtag
    31141 taatgatata aatcctaata tgttaggtat agaaataaca gagcaaattg cattatcagg
    31201 ttcacaaata attattgaac gtatcaatgc gatacataac ttgggaataa agttaattat
    31261 ggatgatttt gggatggggc atagctctct tatatatctt caaaataata actttgatat
    31321 agttaaattg gatggttcat tagttaaaga gatacttaca aataaaagaa gtagtgaaat
    31381 tataatgact attgtaaatc tttcaaaaaa tttagatttt gatatagttg ttgagtatgt
    31441 tgaaaatata gaccaaaggg ataagttata tggtttagga tgtgaaatat atcaaggata
    31501 ttattatagc aaagcaatac cttttgaaga gtttgtagaa tatgcaaatc aagaaaaaga
    31561 gatataattt tataagagct tgtaaattct agtaatgcat agatttgtaa taaaattaag
    31621 tttaggagaa atatatgaat ataagtatag tagtagtagg taaaattaaa gaaaaatatt
    31681 taaaattagg tattgatgag ttcaaaaaaa ggctttctaa gtattgtaag ttagaaatta
    31741 tagagttgga tgatgaaaaa gctccagaga atttgagtgt taaggaaatg gaaattataa
    31801 aagacaaaga aggaaagaaa attttaggga agataaagca taatagttat gttatagtct
    31861 tggctattga tggtaagggt ttatcctctg aagaacttgc taagactatg agtgatttag
    31921 cagttagagg taatagtagt ctctgtttta taataggagg ttctttaggg ctttctgatg
    31981 aggttttagg aagggctgat tataggttat ccttttctaa gatgactttt cctcatcaga
    32041 tgatgagatt gatattgtta gaacagattt atagggctta taggataaat aatggagaac
    32101 cgtatcataa ataaatgata cggtttttgc taatttgaca cttaataata ttaaaataac
    32161 aaaaaagtat tgaaagtata ttgcatttaa ggtagaatta aataaaatat tataacatta
    32221 taatgtttat atagttgtta ataaaaattt ggaggaaaat atatgataaa aataatgttg
    32281 gcctgttcag caggaatgtc tacaagttta ttagttacta aaatggaaag tgcagctaaa
    32341 gaaaatggta tagagtcgca aatttgggca ataccagaat caactataca aaatgaaata
    32401 gaaaaatgtg atgtattatt actaggacca caagttagat atgtattacc taaggctcaa
    32461 gaaattgcta agccatataa tataccagta gaggttataa atatgatgca ctatggaaca
    32521 gttaatggag aagctgtttt aaatagagcg atagaactta acaacactaa gtaatataaa
    32581 ttataatgta tttttaatat ataaattggg agaatcaaag gatggatgaa aagattatag
    32641 aaatatcttt taatataata ggttatgcag gtgaaggtaa agggttagct tttgaagcaa
    32701 taaaagaggc taagaatgga aatatagaaa aagcaaaaga attattaaaa gagtctaagg
    32761 aagttataaa taaagcacat agatatcaaa cagaattaat acaaaacgaa gctagtggaa
    32821 acaggactga aataagtgtc atacttatac atgcacagga ccacctaatg aatggtatga
    32881 attttcaaca gttagctgaa gaaatcgttg atttacattt gaaattacaa gataaataaa
    32941 aattaaagag aataaattaa gagagttctt aaaagataca ttatgtgtag cacactaatt
    33001 gttagattat tgattcaata tttagtgagt aatacattag gattattttg aggactcttt
    33061 ttatatttag tttaaagtta ataaatattt attattaata atataagatt atatgttata
    33121 atattataac atataattta agaggtgaat catgttaaga aatcaaccat tatatataca
    33181 agttgtagat atattaaagc aaaaaatatt aaataacgaa tataaaattg atgaaaatat
    33241 tccaacagaa agagaattag agcaagaatt tggagtcagt aaaattacta ttagaaaagc
    33301 tattgagata ttagaatcag aagggtatgt agagaaaaaa agtggaaaag ggacaaaagt
    33361 aataagtaat agtcttttta ataagctatc aaaagcagag tctttttcaa gtatactttt
    33421 atctaaaggt cataagttat ctaaaaagac tatttcaatt gaaaaaataa taaatgatac
    33481 aaattcacaa gtatttagta tatttggaga aaaatgtagc aagattacta gaatgtatta
    33541 cttagatagt aagccatata tattttttac tcattatatt ccttttgatg aaaatctaac
    33601 tgttgaaagt tttaataatg aaagttcttt atatatgtac ttatatagga gaaatataaa
    33661 tataactaga tttgaagatg aattttttat agaagaatca gaactagagg tatcaaaggc
    33721 tctaaatcta gctaaagaca tattattagg aagaaaaaga aagacctatg atgagaatga
    33781 taatatagtt gagctatcat ttgcaagata taatacagaa gtacaaaact acgtaattaa
    33841 atacgaaata taataagaat cctgagttta ttgaaatgat aatatgctca ggatttttgt
    33901 ttagttgtaa aaacgaaaaa tattataaca ttataatgtt ataatatttt taataaataa
    33961 gtgatattat caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa
    34021 taaaggataa tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga
    34081 gcgcaagatt atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag
    34141 agcaggtata atagcaacat taccactaac aatagtgggg tcatttttca caatattatt
    34201 aaacatacct attgatgctt atatggattt tatagctcca tttaaagcaa cattagatat
    34261 accatttaga tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc
    34321 acttgctaaa agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt
    34381 tttagtttct acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc
    34441 tggtagatgg atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac
    34501 atcattaatt acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat
    34561 gccagcagga gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt
    34621 aattttaatg ttattctggg taataagaca tattttagga tttgatataa gtgttgcact
    34681 aagcgctatg ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggct
    34741 actgacagta ttcctaataa ctttattctg ggtacttggg atacatggtc cagctataat
    34801 ggggccagta ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac
    34861 agaaggagta tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta
    34921 tgtatggata gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa
    34981 gtcacaatat ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa
    35041 tgaacctata atatttggag caccaatagt tatgaatcca atactaggga taccatttat
    35101 aattggacct ataataatga caattctatc atatgttctt actattacag gggttatacc
    35161 tatgatggta gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac
    35221 taactggagt atactagcag gtatcttagt attaatcaac tttgtgatat catttgctgt
    35281 ttattatcca ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat
    35341 gagtgaaaat ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa
    35401 aagcttataa gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat
    35461 attttaaata atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat
    35521 atccagagca tagtaacatg gaagatgata aggcatatat aaaaatggtt tcaaaatacg
    35581 gatttaaaag aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa
    35641 aacattttaa agagattata acctatgcaa gagaattagg atttgaagta atccttgata
    35701 tagctccaaa catatttgat atattacaaa tatcttatga tgatttaaca ttctttagtg
    35761 agttaaatgc tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga
    35821 tttctttcaa tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact
    35881 tggataatat aattacttac aagccaaatg taaataatat atatggttgt cacaattttt
    35941 atcctcaaag gggtacaggc ttgccatatg atttttttgt aagatgctca aatagattta
    36001 aaaagtatgg aataagaact gctgcttttg tcaattcaaa atacgcaaca atcggacctt
    36061 ggaatataaa tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag
    36121 caactaagca cttattcgca acaggattga ttgatgatgt aattgtagga aatgcatatg
    36181 caagtgaaga tgaattaaaa agaatgtctg aagtaaatga ataccaagtt gtatttaatg
    36241 tagtagctaa agaaaaaact tctgaagtag aaaagataat aatggtaaat gagcaacact
    36301 ttagaagagg agatataact aatcaggtag caagatctac agaagtaaga aaaaaatatg
    36361 ctaaagaaaa tttccctaaa catgataata agaacatctt taaaagagga gatgtagtga
    36421 ttggtaatga tgactttgga aagtataaag gggaattaca gttgatactt caagattgta
    36481 cagatgaaag aaaaaataag gtgggtagaa taaaggaaga cgaaataata cttttagact
    36541 ttattgaacc ttggagtaaa tttaagatta ttttagaggg agagtaaagt gaagatagac
    36601 atattaataa aatgtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac
    36661 gataaaataa tagaagtaaa aaatcatata gatgattcta agtatcaggc aaaacaaatt
    36721 ataaacttag atggagagaa atttattagt gcgggttgga tagatataca tacacactgc
    36781 tacgaaagac tagaccttta taaggattat ccagatgagg ttggaataaa aagtggagtt
    36841 actactgtag tagatgcagg aacaacagga gctttagata taggtgaatt ttatgaagat
    36901 actaaaaagt ataagaccaa tgtatatgct ttaataaata ttgctaagca gggaataaca
    36961 tctcaagatg aactttcaaa tatgatgaat attgatgaat atgaacttaa aaatgcagtt
    37021 agaaaataca aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt
    37081 tcaaatgatg ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct
    37141 atgatggttc actttgggag ttctcctcca acgatagagg atatatttga ttatatggaa
    37201 aaaggagata tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat
    37261 gaggttaaaa aggaaatcat agaagctaaa gaaagaggta taattcttga tgtaggtcat
    37321 ggaaccgaaa gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca
    37381 gacactataa gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta
    37441 tctacaacaa tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa
    37501 gttactaaaa atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga
    37561 tatgatgctg atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct
    37621 ttgaataagt cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga
    37681 gaatatttaa atataggcaa ctctatagaa gagaatgagt agaagggaga aactattgta
    37741 tgagtagtat atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga
    37801 ctatactagg agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta
    37861 aaaatttcct tataatagaa gaacttgtaa ataaaacagg tgaatatata gcaaatttat
    37921 tagggtgtga aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg
    37981 gagcaatgat agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac
    38041 aagttaaaag ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag
    38101 aagtaatgat aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta
    38161 acaaggaaca tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat
    38221 ctcatcattg tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag
    38281 aatataattt acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg
    38341 aattgggagc agacctagtg atatacagtg gtgctaaggc tatagaaggt cctagctcgg
    38401 ggttagtaat aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag
    38461 gaagagctat gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat
    38521 atataagtaa tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag
    38581 acaagataaa tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag
    38641 aaatacttcg tggagaaatt aactttaatg aagatataat aaataagtca acacaacaaa
    38701 ttataaatga acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg
    38761 aaggcaagat tgagattgat ataagaagtg taactggaag tgatttagat aatatttata
    38821 ataggataaa aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta
    38881 ataaattgaa ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa
    38941 aatagagttt gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat
    39001 gcctgtgatg gacatgtact agtaggagta ctatcaaaga attatagcac tgtagaagca
    39061 gctattgaag atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtgggtctt
    39121 ggagctggag acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca
    39181 caacatataa atcaagtgtt tacaggtgtc gctacatcta gagcattgct tggtcaaaat
    39241 gatagtataa taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc
    39301 acaggtccat taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct
    39361 atgatgaaag atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca
    39421 aaggatgaat atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca
    39481 acagggggta tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt
    39541 gtagaaaaag ttatacctca tgtttataca tcaataattg ataaagaaac aggtgatacg
    39601 aaagtggaag atataggagt actttataaa ataatgaaag aattattggg atagatttat
    39661 gaatatatta gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt
    39721 aacacaaacc aatgaactaa attatataat tacaggcacg ggattaaata tattatctgg
    39781 acttaaaaac tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg
    39841 taaagcatca agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg
    39901 agggaatcat ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt
    39961 gacttatttg aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat
    40021 tgataaatgt ttagaagaaa tggatatagt ccatatttgt ggtattgcta tgcaacttac
    40081 agattcagtt aaaaagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa
    40141 agtattcttt gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt
    40201 agtaaatgat tataagaaaa tactaccata ttgtcatggg ctgtttgcta gttatagaga
    40261 tataaataag atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa
    40321 aaaagagtat gatatagata ttgtagctgg aactatgaga agatttgatg atatgaataa
    40381 tagatttata aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa
    40441 gttagaaata tatgacagag ttggtgctgg ggatggatat gtggcaggaa ttatatattc
    40501 atatttggaa aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt
    40561 agctcataca acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagactgat
    40621 gaacaatgaa aaaatagata tattgagata atacatttgt atataataaa atttttatct
    40681 tttttagaca cacttttgac ttgcttatat ttgttaaaca gagttatttt taacgattat
    40741 aaatattaaa ttaaaagtta gataataaaa tagtagaaaa tagcattaac catataaaaa
    40801 tggttaatgc tatttttata gttaatattg attaatttta tattatagaa atgaactttt
    40861 ctaatgctgg atttgtattt tttttattcc atacaaccac aacttcaatt ctattttcta
    40921 cttcttttat aggtactatt gcaatatcac gttctatcat tgtatctgta acacaaaatg
    40981 aaggtaaaat ggatattccc atgttagaag ataccaatga ttcaatagta ttaagattat
    41041 ttgaacgttg taccacatta ggtacaaacc ccgcttcacg atatctattt tgtatcattg
    41101 acttttgtcc atactcatcc cctgtttctt ttacaaaaat aaatttatca tgttgtaatt
    41161 caattaaatc gatagaatcc tcattgtaaa gtggatgacc aggaggtaaa agagctacca
    41221 aagaatatct ttctacagtt ttatattcaa aatcttttaa tggttgattt tctggattaa
    41281 taacaaaagc gaggtccata gaattatttt ttactaagtt taaaagttcg gttatttcat
    41341 tagtacaaat atccatagat atattagggt atgtattaga aaattctttt aagtattgtt
    41401 gaagtttgtt tttttcatat cctattaagg ttccaatgct caaagttcca aaaaaaccat
    41461 gtgcaaattg ttgtgttttt gcaatagcat cattgatatt ttttattata gtacgtgcct
    41521 cagataaaaa aacttttccg gctggtgtta gttcaacatg acgtttactt cttgtaaata
    41581 gagttacatt tatttgttct tctaaagctt ttatttgctg tgtaacagca gtttgagaaa
    41641 tataaaattt ttctgctgtt ttggtaaaac ttagattttc tgctacagat ataaaatatt
    41701 ctaattgttt agtattcaaa taatcacccc tatttaaact tgattgtttg taaaccttat
    41761 ttatctataa ttaaagctta ttatagtata agaaaattta cttttaaaga atattagaaa
    41821 atatttgtat aaaaatatat gttttaagta tatctttaca ttagatttta taaatttatt
    41881 aataagaata acttatatat tccaatgaaa acgatattgg atttaagtaa gtttgtctca
    41941 taaaatgaaa gtatatcaat tgataatact ttaaataaga agggagaaaa aatggaagat
    42001 aaattttatg caaaaggcaa cggaaataac ggatatatta aaaatcttga agtttgttcc
    42061 tttaataact tagatggaac ttgtggaatg tttcaaatgg ctctgtacaa aagagatgaa
    42121 aaatactatt tatatggatg ctgttttgga ggaaataaaa aaaatggagt aatgattagc
    42181 gatattacag acccttataa tccacaattt ataaaacatt ttcaaatgtt agaccctaaa
    42241 gagtatccta caacaacaac tccaaaaatt caaatagcag atgatttaat gatagtagca
    42301 atgagttgtg gaagtggacc aggagcactt gttgaccaag ctaaattagc aaatattaag
    42361 tgtgaagcag gaattagaat atacagttta aaagaagacc ctttaaatcc taagttttta
    42421 ggatattggg attgtggctt aaagcatgta atgggtgttc atagattcat gtacaatggt
    42481 ggaagatatg tacatttatc aagtgattgt gttggctttg aaggtctgat ttatagggtc
    42541 atagatataa taaatcctaa taatccagtg gaaataggta aatggtggag accagaccaa
    42601 tatgcagatg gatatccaaa tagaactttt gatgcaggag cacctcattg cccagaattt
    42661 atggataaag gatggcttca tggacctcca tttgtaagag acggaaaagc atattgtggt
    42721 tatggaggag ctggtttagt tgtattagat gttgaagatt taacaagacc aagatgctta
    42781 ggtgaattgc catttatgcc tgcattttct agtagacttg caggtgcaag aactcataca
    42841 gcattaccat tgccaggaag agatttagtc gttgttcaaa atgagggaga aagattccag
    42901 ttctttaaac cagataacat tacagatgtt caagctatga ataatataca tatggttgat
    42961 gttagtgacc caacaaaacc aacattaatt gctcaattcc catatcctga agttccaaaa
    43021 gatttccctt atcctaactt taatgttgca ggattaggaa aagcagggcc atttggtcca
    43081 cataatcttc atgaaccaat ggataataag ccatggttag agcaaagagg agatagagta
    43141 tattgctgtt atttccatgc agggctaagg gtttatgatg tatcagaccc atattatatc
    43201 aaagagctag catattttat accaccaaat ccaaataaaa caccagaaga atcttatttc
    43261 ccaggattcc caggaccacg cttggcagta acagaagatc ttatcgttga tgatagaggc
    43321 tacatcatca tagatgcttt agatgatgga ttctatatat taaaaatgaa agaagattaa
    43381 ataaatacta aagagggaga gttgaaaatg aaaatactag gtatttcttt tggaacaaaa
    43441 aatggaaata atgacacaat gtgtaaagtt gcacttaaag gtgccaaaga agcaggggct
    43501 gaagtagaat ttattcaaat gtcatctatt aacattaaac attgtactgg atgttgtgcg
    43561 tgcgttaaaa ctttattatc cgggaaaggt agtatgtgtg tattaaaaga tgattttgaa
    43621 tggttgcttg acaaaatgaa agatgcagat ggtatagtag tttcagaccc tatttttgaa
    43681 gaaggggcat caggattatt ccatactata atggaccgtt ttggacccag agcagataga
    43741 ggaaataaca ttataggaac aaaagttgca gaagctatag gaggaaaaat tccagactca
    43801 agaatgctta atgataaagt aatatcattt atgggaatag gaggttctga ctggggtaca
    43861 agagttcaat gtgaacatgc tatgctagct ctcataccaa tgtggaaagt tatagataat
    43921 gcttggttcc catgggcaaa ggaattagta atggatgata caaggcttgt tcaagttcat
    43981 caaattggtt taaatatagt tgaagcagca aaagattttg aaaaagcttc atatcaagga
    44041 gaagaaggtg tatgtccaca ttgccacaat agattattct accttgagtc aggtactaaa
    44101 aaggcaattt gtgctctatg tggtatagtt ggagagttag atactataag tggaaaaact
    44161 atatttagtt ttccagaaga gcaattaggt catgctcatg atactttacc aggtaagttt
    44221 atacatggtg atgatattaa gaaaatggaa ggtcattatg cagaagttcg taaaacacaa
    44281 gaatttaaag aaagaaaaac atcttataat tttatagagc cattaactaa agaaaagcaa
    44341 gctggagttt cattaggata ataattaaaa tactaagact cttttttaag ggatgttaga
    44401 tgttatagga ggcttattgt atgaatgaaa agccaagttt taaacaaatt gaaattattt
    44461 tagcagttat ttttttggta cttacatatt acaagattgg attaactcca gcatttattg
    44521 ttatatttat accattatac ttaagttatt ttgcagtatc tatgattgta aatcaaatag
    44581 caattaagct attggagata aggcaagcta gggaagctac taattacaat ataggaaatg
    44641 gatagaaaga aaaaagaaat attaacactg tctatgggga ttgctttact acctccttta
    44701 tgggcagtat tagcacctta tataggaata aaaactggag ctgtagcact aatctgtgca
    44761 ggattatatg taactaatgg taataaacaa aaagatggat taaaaataat gtttggtttt
    44821 tggtgtggag atttatgggc agtgttagcc attttaatta tgggatatat gaatttcaat
    44881 caaaacctag aattattttt aacacttagt atattaggtt tttttgcagt tgtaattgca
    44941 tccttatttg aaaaaataat tttcctacca tcatggcttt gtggttgggc tataggtcta
    45001 actattatga caggtgaaaa tattattaat ttacaagata tttgtattca aattgctgta
    45061 gctatggcag taggagtatg gtacgtgggt gcaggtgtag atttgtttac aatatatatt
    45121 ttgagtaaag ataagaaaaa gggaaattga aagcatgtga atatactttg attataggag
    45181 aatggctatg ggttttgaat ttaaaataat gagaagttta atatatgtag gacttgccaa
    45241 ggaagaatat agacctaagt taatggactg gttatatcgt caccatattc cagatagtat
    45301 tagcactttt ggaccatatt gtactaaata tgccttttat caagcatatc ctacaccaaa
    45361 tgaaggtgag cgttttggtg cacgtaagat gcaactaaca gaacattatt ggcttgtaga
    45421 tgaacatatg cctgagatgg caaatagaat tatgacagaa tatatgccta tggatgttct
    45481 acgttggcaa gggtgtatac cagatgtaga aaataaaagg gttcatgaaa atgcagaaag
    45541 tggagatgca ggacgtgcag taggtggaga taatggatgc ccaccattta tatttgcttt
    45601 tgttccaata aactgggaag aagactttag aggaaaagga cgtactgtac aagatggacc
    45661 aaactatcgt tggcaattta tgattaagta tccagatggt atctctaaag aagaaggaga
    45721 aaaatggttc tatgatgagg tagtgccata ctttacaaac tgttgctatg ttaatcgttt
    45781 tgtcagtagt aaaataatga ttaattatgg agcaactgct tttgaccgtg tatcagaact
    45841 atggtttgaa ggggaagagg aatggtataa agctgtggtt gaagagacaa agtcgtttat
    45901 taaaaaacca gaatgggcac aagaagagga gttcccatat ttaaaaccac aattcaatat
    45961 cgcatcagta ttcttaggtg atatagcaac tatggatgca tactcacagt atcgtggata
    46021 tataccaatg agataaaaat tatagtttgt gataagtaga ggaagtactt actcttactg
    46081 ctagaagaag acttattgta cacttaaaag aatgaaatag ataagtgtca atttattata
    46141 tgagagaaac tttaactttc ttggtttaaa atattaaagt cataaggaac atttattatt
    46201 aaatacttta tttatatgaa ttaattgaag aaaaaggata ttaaactaca agttaaaaaa
    46261 taaatttaat atattagaat tataaataag gcacattaat tttattttaa attaatgtgc
    46321 cttttattta tggtaattta tgcataatta gtatcgcttt gattctttaa ataaattttt
    46381 tttggtttct ttacataagg aacccttttt tatttataaa atctataagt gtaatatagt
    46441 agctatttta gataagttta atttttgtct caatctaaaa caaaagttat acttaaatga
    46501 aattgaaaac gataaaattc tcaaataaaa taagtattgt ggaggtgatg aaagaatcca
    46561 atgataatat gaagactaat cttaataaga aagtaggggt taatggtagt ggacaaagaa
    46621 attgtatcaa ttctccagat tttattaaat agcacattga ttacaactaa tggattacaa
    46681 gaggagtcta attcatcaaa gagacaaatt acttatagaa taaataaaat caatgacatg
    46741 cttaaagtaa aaaatgtacc acttatatgt ttaagagctg ataaagatat tatcgttaga
    46801 aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa actattctaa gaatacttat
    46861 tattttagta agacagaaag acttttatat atgtatttaa tgctttttat taacatggag
    46921 gatatttcaa ttaatcattt tataaattct ataaaagtaa gtaggtctac tgtaaatctt
    46981 gatttcaagg atttgatacc agagcttgaa aagaagaaca ttaaagttaa gaacaatcgt
    47041 ataaatggat attacatagt tggcaatgag atggaaatta ggagagtgct tattaaaaac
    47101 attatagaaa ctttgtctaa tgagaaaagt tcaagagtat ttgatatttt tattaaagaa
    47161 tataatttag atagttttga agaggctaaa aaaattatat taaagctaat aaaaaaatat
    47221 gaaattactt ttgttgaaga tagattaata gaatttatat atatatttat ctttctaaaa
    47281 gcaaggatgt attcaactag aatagttact caggaaaatg tagatattcc taatatttca
    47341 gttatgagtt caatgaagga gtataaattt gctaaggaat tattagaagt atatgggagt
    47401 gaggataaga taaagcctta taatataatg tatattagtg cttggatttt aggtatatcg
    47461 gtaggaaatt atgaagaaga tacagaagat aaggatgtaa tttcaaaaat tgtaaataaa
    47521 atgatgacaa agtttggata tttaagcgga gtttactata taagccaaga aaaaatattt
    47581 aaacaattat attctcattt tagacctgcc tactatagac ttttatttaa attacctatc
    47641 tataatccga tttgtgaaaa agtaaaagaa gaatataaat tagtttatag aattgtaagt
    47701 aatgcaatga aagagttttg cggattattt ggagaagaaa ttcaggaaga agagttagca
    47761 tatcttacaa tgcactttgc aacacttttt tcaaataaaa aagaatttga tggttttaga
    47821 aaaagaatag cattaatagt ttgttcaaat ggcgttggta gttcagctat tttacatgca
    47881 gaacttacaa acttatttcc tgacttacac tttataccaa tgtcggaatc agttggatta
    47941 gaaaatataa gtgaacctgt agatatcgta tttactacta attataacgc agagggttta
    48001 aaaacagatg taccagtaat taaagtaagc cctgttatga ccacaaaaga aaaatataag
    48061 attacacgtg aagtgtatat tcaattaggt gatatttatt taagacaacc taaaatagat
    48121 gaggttatgg atataataaa aaaatatgca aatgtaacct ctgaaagtat tctttcaagt
    48181 gagctattag cttattttac acagatagag aattttacat ctaaggaagg agaggggcct
    48241 atgctaagtg agattactaa tgaaacacta gtaaaaataa aggtaaaagc taagaattgg
    48301 gaggaagcta ttagacagtc agcttctgta ttagttgaaa ataacaaagt aactgaaggg
    48361 tatgtagatg ctatgattaa ctcagcaaaa gcatctggac catacatagt aattacaaaa
    48421 catgttgcac ttccacatgc tagaccagaa gctgggtcaa aagagattgc aataggcatt
    48481 gctactttag atagtcctat tgagtttggg aataacgaaa acgaccctgt aaaatatgta
    48541 ttttgtctaa gtgcaataga caataatagc catttacgtg ctatgtctga gctagttgaa
    48601 ctattggaag aagatgagtt ttttaaggtt ttagatagtg ctaaagaggc taaagaaatt
    48661 attgatttta taaaaacaca cgaattataa aggaggattt aattatgtat aaaattttaa
    48721 tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa aataaagact caacaaatta
    48781 ttaaggaaaa taactttcca attgaagtag aacatggttc attggattca ctaaatggat
    48841 tcaatggaga tgctgtagaa acattaattg atgttgctga agaattaaaa aataaaaatt
    48901 tgaagtttga tataattggg attcacaata ttgttgaccg taatgaaata aagacagcac
    48961 tagaaaaatt ccttgcttca aaagagggat aaatttaata tgataaggag gaatatttat
    49021 ggagtttatt attagtttgc tgagtacacc tgctgttttg cttggcgtag ttgcatgtat
    49081 aggattgatt cttcaaaaga aatcagccgt tgaagtattt acagggtctt caaaaacact
    49141 tatcggtttt ttgatatttg gtattggtgc ctctgctatg acaggagcac ttcaaaattt
    49201 caataaactg ttccaacatg gctttggaat tactggtgtt atagcttcac cagaagcagc
    49261 tacagcactt gcacaaggta catatggttt tgcagtttca tgtacattaa ttcttggatt
    49321 catattaaat ttagtatttg cacgtattac aaagtttaag aatattttct ttacaacagg
    49381 tcatagttta ttcttttctt gtgtattagt tttaatcatg aaagcacatg gatttgataa
    49441 tacaatcact attttaatag gtggaactat cttagggttt atgtctgctg cactacctca
    49501 attctgtcaa ccatttatgc gtgaattaac aggtggcgac gaacaagcta ttggtcactt
    49561 taacatgatt ggttatggtt tatcaggtta tattgggcgt ttattttcaa aacataaaga
    49621 tgatacaaca gagactatag aatttcctaa gtggttatca atattcagag acttcttaat
    49681 gggtctatcc atagttatgt taatcttatt ctatattgca acattaaaag ctggtcaagc
    49741 atttactcaa gaaattgctg gttcaacaca ttggttggtt tttccgctta tagaggcatt
    49801 tacatttgtg gcaggtatgt caatattgat gtcaggtgtt cgtatgttct tagctgaaat
    49861 aactgcagca tttgtaatca tttcagaaaa gtatattcca ggttcacgtc ctgcgctaga
    49921 tgttccaaca gtatttccat atgcaccaaa tgcagttatt attgggttta tatcagcata
    49981 tgctgctggt ctattagcaa ttgctattat ggcatcattt cctagtatat ttcctgtagt
    50041 aattattcca gctgctcaca tatgtttctt ctcaggaggt acagctgcaa tatttggcaa
    50101 tacttcagga ggatggagag gggctattgc tgggtcattt gttgttgggt tattacttgc
    50161 atttttacca gttatactat atccagttta tgggactctt ggtatagaag gagcaacttt
    50221 ccctaatatt gattacaacg tagttggttc gatattacat aatattttac aatttataaa
    50281 acctatttaa actaaattat ataaaagtat agtcatagtt actatgattt caaggaggat
    50341 acatcttatg gaaaatacat tatctgaatt tttagataat atatctaatg aattgtcaga
    50401 atttattgcc tcaatagatg agacatctat aaataaagca tgcgaattaa ttcttgaagc
    50461 tgaaaaaaat cacggacgtg tacatgtgac tggtattggt aaaccaggac atgtatcagg
    50521 atatatttca tcattgcttt catctactgg aacatcagca tatatattgc atggtacaga
    50581 agcagtacat gggtcaagtg gtcaagtagt tgaaggagat gttgttattg caatatcaaa
    50641 tagtggtgaa actcaagagt taaaagcaac tcttaagact ctaaaagtaa atggagcaaa
    50701 aataattggt gtaagtggta atgagagttc atttttaaaa aatatttctg atatattcct
    50761 gttcgcaggc gttaaacagg aaggagattg tcttaataaa gctcctcgtg cttcaatttt
    50821 agctgaaaca atagtattac aatcattaag tgttgtatta caatatgcta aaggtcttaa
    50881 tacacagcag tatttgaaat ggcatccagc aggaagtctt ggaaagtcta ttagagaggg
    50941 aatataattt tatgaaatat aaagggatta tatgtgcaat gataacacca tttgatgaaa
    51001 agcaaaatat taattcacaa gcaacatgta aattaattga ttatttgatt gaaaagggga
    51061 tttatggatt atttattttg ggtactaatg gtgaatgtca tgtgttaaca gatgatgaaa
    51121 aagttgaatt tgcaaagatt gttattaatc atacaaataa tcgtgtacct gtttttgttg
    51181 gaacaggtgg taattcaact cgtgaagtta tagatttatc caagagaatg gaagcgattg
    51241 gagcaagtgc attatctatt attacaccat actttgttac accaacacaa caggaattaa
    51301 tcttacatta caaaactatt gctaattcag ttaatttacc tattataatg tataatatgc
    51361 caggtaagac tggaattaat attgaaccag agtcagtacg tgaattatca aagataaaga
    51421 atattgttgg aattaaggat agtagtggta aacttgacaa catgaaagct tatattgaag
    51481 taacgcagaa tgagaatttt agtgtgtttt caggctcaga ttcacttatt ctggatagtc
    51541 taaaagctgg tagtcaagga gctgttgcag caacaacaaa cttcctcaca gagattggta
    51601 tttctatcta tgaaaacttt atgaaagggg atttggaaga agctcaaaaa gctcaaagta
    51661 gtatagaaga acttcgacgt atactaaaac ttggaacaat tccatctgtt atgaagaaaa
    51721 ctattgtctt gaatggaatt aatgttggaa ctgcacgact tcctgtaact gaaccaactg
    51781 gggaagtact tgaagaaatt aaacgagtag ttgaaaatta taggacaata cttaataaaa
    51841 gtagtgagaa tagataatat aaatagggaa attacactgg tatataatta atatatatat
    51901 tataaaacat gactaaaatt aatgaataag taaagtggtt tgatgagtat agaggtgata
    51961 atatgaaaaa gatgaatata accaaaagaa tgtcagaatg tggggctctt gcaattgtaa
    52021 gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg atgtataaaa ggtggtataa
    52081 cagttataga aatgagttat accctaaata atgctggaga aataattcaa gggcttaata
    52141 aaaagtatgg tgaaatactt tgtgtgggag caggaacagt tttggatagt gaaacagctc
    52201 gtcatgctat attacatgga gctcaattta ttattgcacc aaattacaat gaaggtgtag
    52261 cgaagatttg caatagatat cagattcctt atgctccagg atgtacatca ttgactgagg
    52321 cagtagatgc actaagttta ggagcgactt ttataaaagc tttcccaata tcagatttct
    52381 atggacctaa actagtaaag gtatttaaga caccaatacc tgatatgcca gttttagcaa
    52441 gtggaggaat taatcttgaa aacctacata tatggttaga aaatggagtt gatgtatgtg
    52501 gatttggtgg tttattaact aaaggaacta ttgaggatat tactgaaaat gccaaaaaga
    52561 ttcgtgaaat cattaaaaat ataagagaaa tttaacatgg aggatgaatt agttttctat
    52621 tgacgagata tccacacatt aattcctaag ttagaagaaa tattttctag ttgaggtaaa
    52681 gtttatattg aacttaaatt aaattttgtt ttaaataaga tattatttta gttgattgct
    52741 aaaaatacta gaatctatat taatgaatta tactaatttt gtaccactat taaaatattg
    52801 tttgctaaaa ataaaaaata accttaaaac agttgattat aaaaaattaa ctgttttaag
    52861 gttattttta tctttgtgac gaaatagaca cttgccattt agaatgtgac aatatagtag
    52921 ttgtttagaa aaggtacaaa gcattatact ttaactaaag taaaatatat tttgctttta
    52981 gaataaaaac aattaggagg gagctataat tgaaaaagat aatatatgta ttatttacaa
    53041 ttgaactatt aatagtatct ttattaggtc taaatatagt taaagataat gaaattaata
    53101 atattttata taacgataca acaagtatat ctgtaatgtt taaagattat aaaaagttaa
    53161 ataaaaatta tagtaagtgg attaaagata ttgcagatga aaataatgtg gctatatcaa
    53221 agtatgtttt taataataat gaaaaattat ctatatatac gactgataca agtttgaata
    53281 acaatataaa gttaaaatca ggaaaatttc ctaatactaa ttcaaatgaa tttattagta
    53341 atataaacac taataatagt aataatagta atcaagtagg taaattctca actatgagta
    53401 aagacatatc aattttaatt agggattttg gtaaaataag tgaagttgga gaatcaggaa
    53461 tcttatatat ttcaactcaa aatgaagata caataaataa aatattaaga gaactaaatg
    53521 tagacagaag tatttttgta gcaagattac atgatagata tgttaactca aatttatact
    53581 taaatccttc aataattaga gatttaatac taattatcct ttgtttcttg gctacaataa
    53641 tacattattc aattagtgtg agtagagagg cttctatttt aagattaaat ggatactcta
    53701 agctagatat aattgctagg gttataaaaa atatatttag aattatgatt ttatcatctg
    53761 tcacagcatt attaatatat ataatctata ttttaaaatt aaatataggt gttagagcat
    53821 gcttatactt tgctatgttt tcgattatat gtattttgat aaatattttg atatctatat
    53881 taatagttgg ttttaatagt agaagttcta aatatgtgct atctctaaat ggaaaaaaat
    53941 cttatggttt tgtaaatata atgcatttca cattgaaaat tgtatttgta tcatttctta
    54001 tattatccat aaataactgt attttaaatt ataatatgct acaaactcag ttaggaaatc
    54061 tatctgagtg ggataaagca aaaaatgttt ataatcttac tcttaaagat acaggcgaac
    54121 aatctttagg aaaggaagaa gtaattaaaa atgccaagat taaaggtttc tataaaaatt
    54181 tggtagaaga aaaagatgct ttcttaatag attcaactaa ttttgaaaaa cttgaagatg
    54241 gaagttacat gtatgaaata aattcaaagg gtaaaaaccc agaagtaagt caatatggta
    54301 aaaatataaa aataaataaa aactatctta aagttaaccc tatttactca aatggcaaag
    54361 aaatatttaa tctaatcgat tatgacgata agactataaa tttattagtt cctaaaaagt
    54421 tacaagtaca tgaaaatgag attaaaaaag agtttagaga attattttat tttgaaaaaa
    54481 tagaagtaga aaatatgtac aataaagagt taaatagaga gttaaataaa acaaagaagt
    54541 cagatttaaa tgttaatata atatatgtag ataataatca aagctatttt acttataact
    54601 cccttgttat ggatgacaat aggaatctca ttgatgaccc aatagcaata attgacatag
    54661 ataatattga tgattctttt tatctttctt atatatctag atgtgtatat tttaattcta
    54721 agaaattaga tgcccttgca gatataagta acattattga agctcaaggt gtagaagcgc
    54781 atatccaaag tttacatgct gtttataatg agtatggttt agaaataaat aaactagaaa
    54841 aatatttgaa tagtgaaatt tttactataa taataatagc aatatcaaac ttaatgatta
    54901 cctacaatat agtagctagc tactatgaaa gaaataaata taagttatat attaaaaaaa
    54961 tatttggtta ttcaactaca gctagaagta tgttgttaat agtctcatta atattaacaa
    55021 acattattcc aataggcatt atatcaacaa gagtagattt aagtaataat attatcttat
    55081 ttggacttct gatattagtt atagaagtaa ttgtatctat tgtattagac aagattatct
    55141 caaatagttc atttaataaa attataaaag gagaacatta aaaagatgat ttcattaata
    55201 aatatatcta aaaaatttaa agataaatct ataatagaca aatttaacct agaaatttct
    55261 aagggcgagt ttgtagcgat aacaggaaat agtggttcag gtaagacaac tttacttaat
    55321 ataatgggat tattagaaaa acctaattct ggtgatatta taataaataa tataaaaaat
    55381 ccaaatagta aacagataag aattttacaa agggattttt atggctatct atttcaaaat
    55441 tatgctttaa ttgagaatga aaatgttgaa aataacttga aaattgcttt aaagtatcaa
    55501 aaaaatatca accataatga aaaaataaat tacgcacttg agtctgtagg tcttagaaaa
    55561 tacaataaaa aaaagatata cgaattgagt ggaggagaac aacaaagggt agcattagca
    55621 agagtaatat taaaagaatg tgaagtaata tttgcagatg aacctacagg taatttagat
    55681 aaaaaaaata gagacatagt atttgatata cttaaaaatc taaataagaa tggtaaaact
    55741 atcgtatttg taactcatga tttagagtta tcggagcagg cagatagagt tataaaattg
    55801 taataatatc aactataata aatataagaa agagattaca gctaaatcaa gtttaataat
    55861 aatacgaata gaatttaaac attaagtaaa ttagtagttt gtaaatataa gtataatatg
    55921 atatcataaa ggctcaaaag aaattttata taataagaaa tttaaaaata acaaatggag
    55981 gacatataaa gatggaaaat ttaccaaatt gcccgaaatg taattcagaa tatacttatg
    56041 aagatggaac tcttttagtt tgtccagaat gctctcatga gtgggcatta ggattgggag
    56101 ctgaagaaga ttcaaatatt atcaaagatg taaatggaaa tgtattaaat gatggagatt
    56161 ctgttacagt aattagagac cttaaagtaa aaggaagttc atcatctata aagattggaa
    56221 caaaagtgaa aaatatccgt ttaatccatg actcatcaga tggacatgat atagaatgta
    56281 agatagatgg ttttggagct atgaagctaa agtcttctgt tgttaaaaaa gcgtaatatt
    56341 ataaataagc tgtagtgttt attgtttttc agtacagtat aaaaccataa gaatctatta
    56401 agtaataaaa gtttgtttat ttatctttat attttcttat ggttttatta aatataaatt
    56461 ttattgaaca ccaaaatatt ctctcctcta caaataagcc aatcttattt
//
LOCUS       DAEPOG010000006        54006 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEPOG010000006 DAEPOG010000000
VERSION     DAEPOG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.006"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..663
                     /locus_tag="KR432_000314"
     CDS             <1..663
                     /locus_tag="KR432_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0638872.1"
                     /translation="KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGG
                     SAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKP
                     DLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTR
                     VLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDS
                     LK"
     gene            891..2804
                     /locus_tag="KR432_000315"
     CDS             891..2804
                     /locus_tag="KR432_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638873.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            2797..5016
                     /locus_tag="KR432_000316"
     CDS             2797..5016
                     /locus_tag="KR432_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0638874.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5125..5955
                     /locus_tag="KR432_000317"
     CDS             5125..5955
                     /locus_tag="KR432_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH0638875.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            5945..9412
                     /gene="addB"
                     /locus_tag="KR432_000318"
     CDS             5945..9412
                     /gene="addB"
                     /locus_tag="KR432_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBH0638876.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9412..13239
                     /gene="addA"
                     /locus_tag="KR432_000319"
     CDS             9412..13239
                     /gene="addA"
                     /locus_tag="KR432_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBH0638877.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13285..14496
                     /locus_tag="KR432_000320"
     CDS             13285..14496
                     /locus_tag="KR432_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBH0638878.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14483..18010
                     /locus_tag="KR432_000321"
     CDS             14483..18010
                     /locus_tag="KR432_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0638879.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            18141..19394
                     /locus_tag="KR432_000322"
     CDS             18141..19394
                     /locus_tag="KR432_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0638880.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            19543..20594
                     /gene="ytvI"
                     /locus_tag="KR432_000323"
                     /pseudo
     CDS             19543..20594
                     /gene="ytvI"
                     /locus_tag="KR432_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
     gene            20685..21911
                     /gene="pepT"
                     /locus_tag="KR432_000324"
     CDS             20685..21911
                     /gene="pepT"
                     /locus_tag="KR432_000324"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH0638881.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            22118..23170
                     /locus_tag="KR432_000325"
     CDS             22118..23170
                     /locus_tag="KR432_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBH0638882.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(23506..24075)
                     /gene="yfcE"
                     /locus_tag="KR432_000326"
     CDS             complement(23506..24075)
                     /gene="yfcE"
                     /locus_tag="KR432_000326"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH0638883.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(24100..24900)
                     /locus_tag="KR432_000327"
     CDS             complement(24100..24900)
                     /locus_tag="KR432_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBH0638884.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            25035..25907
                     /locus_tag="KR432_000328"
     CDS             25035..25907
                     /locus_tag="KR432_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0638885.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            25895..26581
                     /locus_tag="KR432_000329"
     CDS             25895..26581
                     /locus_tag="KR432_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0638886.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            26633..27175
                     /locus_tag="KR432_000330"
     CDS             26633..27175
                     /locus_tag="KR432_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638887.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            27254..27721
                     /locus_tag="KR432_000331"
     CDS             27254..27721
                     /locus_tag="KR432_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBH0638888.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            28429..29565
                     /locus_tag="KR432_000332"
     CDS             28429..29565
                     /locus_tag="KR432_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBH0638889.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            29624..30406
                     /locus_tag="KR432_000333"
     CDS             29624..30406
                     /locus_tag="KR432_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0638890.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            30425..31435
                     /locus_tag="KR432_000334"
     CDS             30425..31435
                     /locus_tag="KR432_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0638891.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            31454..32251
                     /locus_tag="KR432_000335"
     CDS             31454..32251
                     /locus_tag="KR432_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0638892.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            32349..33194
                     /locus_tag="KR432_000336"
     CDS             32349..33194
                     /locus_tag="KR432_000336"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBH0638893.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            33281..34456
                     /locus_tag="KR432_000337"
     CDS             33281..34456
                     /locus_tag="KR432_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0638894.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            34651..35553
                     /locus_tag="KR432_000338"
     CDS             34651..35553
                     /locus_tag="KR432_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0638895.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            35567..36571
                     /locus_tag="KR432_000339"
     CDS             35567..36571
                     /locus_tag="KR432_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0638896.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(36715..36942)
                     /gene="acpP"
                     /locus_tag="KR432_000340"
     CDS             complement(36715..36942)
                     /gene="acpP"
                     /locus_tag="KR432_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0638897.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            37114..37539
                     /locus_tag="KR432_000341"
     CDS             37114..37539
                     /locus_tag="KR432_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638898.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            37587..37793
                     /locus_tag="KR432_000342"
     CDS             37587..37793
                     /locus_tag="KR432_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638899.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            37975..38154
                     /locus_tag="KR432_000343"
     CDS             37975..38154
                     /locus_tag="KR432_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638900.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            38188..38388
                     /locus_tag="KR432_000344"
     CDS             38188..38388
                     /locus_tag="KR432_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638901.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            38641..39657
                     /gene="ccpA"
                     /locus_tag="KR432_000345"
     CDS             38641..39657
                     /gene="ccpA"
                     /locus_tag="KR432_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBH0638902.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(39780..40178)
                     /locus_tag="KR432_000346"
     CDS             complement(39780..40178)
                     /locus_tag="KR432_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638903.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            40390..41295
                     /locus_tag="KR432_000347"
     CDS             40390..41295
                     /locus_tag="KR432_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638904.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            41410..41590
                     /gene="ssrS"
                     /locus_tag="KR432_000348"
     ncRNA           41410..41590
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KR432_000348"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            41773..42990
                     /gene="cdeC"
                     /locus_tag="KR432_000349"
     CDS             41773..42990
                     /gene="cdeC"
                     /locus_tag="KR432_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBH0638905.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(43091..44413)
                     /locus_tag="KR432_000350"
     CDS             complement(43091..44413)
                     /locus_tag="KR432_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0638906.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(44464..45258)
                     /locus_tag="KR432_000351"
     CDS             complement(44464..45258)
                     /locus_tag="KR432_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0638907.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            45368..46813
                     /locus_tag="KR432_000352"
     CDS             45368..46813
                     /locus_tag="KR432_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0638908.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            46842..47051
                     /locus_tag="KR432_000353"
     CDS             46842..47051
                     /locus_tag="KR432_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBH0638909.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            47071..48465
                     /locus_tag="KR432_000354"
     CDS             47071..48465
                     /locus_tag="KR432_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0638910.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            48705..49457
                     /locus_tag="KR432_000355"
     CDS             48705..49457
                     /locus_tag="KR432_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0638911.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            49483..49896
                     /locus_tag="KR432_000356"
     CDS             49483..49896
                     /locus_tag="KR432_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0638912.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            49900..50643
                     /locus_tag="KR432_000357"
     CDS             49900..50643
                     /locus_tag="KR432_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBH0638913.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            50643..51113
                     /locus_tag="KR432_000358"
     CDS             50643..51113
                     /locus_tag="KR432_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0638914.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            51130..51888
                     /locus_tag="KR432_000359"
     CDS             51130..51888
                     /locus_tag="KR432_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0638915.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            51872..52714
                     /locus_tag="KR432_000360"
     CDS             51872..52714
                     /locus_tag="KR432_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0638916.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(52874..53779)
                     /locus_tag="KR432_000361"
     CDS             complement(52874..53779)
                     /locus_tag="KR432_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0638917.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT        21928 a         5592 c         9582 g        16904 t
ORIGIN      
        1 aaaataggtg gaggattgac ttctaaggta atgagctcta tagcatcatc attatctaaa
       61 cacaatacga ctccaacaga accaggaaat agtgggggca agacagttat gattgaccca
      121 gggcatggtg gttcagcacc tggaaattca tctggaggaa tgattgaaaa agattacaat
      181 ttaaatactt cacttgcaac aactgaatat ttacgttcaa agggattcaa tgtaataatg
      241 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctctatc taattcattg
      301 aaaccagatt tatttacaag tatacattat aatggctcaa ctaataaaca aggtcatggt
      361 gtagaagtat tttataagct taaagataaa aatgggggga ctactaaaac tgtagctacc
      421 aatatattaa atagaatttt agagaaattt aaacttacaa atagaggtat aaaaacaaga
      481 gtacttccta gtgattctac aaaagattat ttatacgttt taagaagtaa tgatatgcca
      541 gctgtacttg tagaatgtgc atttttggat aatgaaaatg atatgagttt aataaactca
      601 tctgcaaaag taaaagaaat gggtacacaa ataggtaaag gaatagaaga ttcattaaaa
      661 taaatggaaa taaaaatatt taaaaactta atgtaaattt atactaaagt caaaaaatga
      721 aatataagtg tatcaatagt aagactgtat gtacatttgc acatgtgaat gcaagtatat
      781 atacagtctt tttttgtagt taaatgcaat aatttgattg tgttttgtaa aatacacaat
      841 atgatataat tagataatat acacttaatt taattgagga aggtgattat atggaatatt
      901 tatttgaaaa aagtagttta aaagaatatg gaaatgaagc tttagataaa tatagagaat
      961 tattagataa aactgataat aattcaaggt gtattctttt attagtacca aacaataaca
     1021 caaagatacg atatgaaagg gctctaagat taaattatag tgaagaactg aaaataacaa
     1081 catatataag ttttgtgaag aaagaattag ttaaatactg gccactaata atagaaaaat
     1141 gtgatgaaat aaatacaaag gttgtatctc caacatttat atcaaatagt ttaagtgaat
     1201 atttaatagt gcaaaaagtt aaggaaaaaa ggatacaaga aggatatttt gaagatatta
     1261 caggaaccaa taaatccata gcaaatagta taatgactaa cataaataaa tctgcattta
     1321 atttaattga ttttgatttt attggagaaa aaatatattc atcaaagaaa aataaagata
     1381 gtatatatag attttcatac actcagatgg atgaaattat atcatattat ataaattcgt
     1441 tactatcaaa ttcaatttta gataatgcat taagcatata tttatacaat caatacctac
     1501 ttaatgatga tttttattta aaaaacttat ttacagaagt gagatatata attgtggata
     1561 gtcttgaaag ctcaagtaat gcagaagttg attttataac agaagcattg gataatacat
     1621 tacaaagtta tatatatttt gattatagta gggattattc tgtatttaat aatgtggata
     1681 tagactatat aaatgagaaa ataatatcaa aaattaagcc taaagaagaa aatattaaaa
     1741 agaaggagat tagaatagaa gatttatact tattacctgc aaatatagaa ttaaatcaat
     1801 caagtcaatt atacaatgaa atgttagatt taataagtga aaaagttata tctcttatag
     1861 aagaaaatgt atctccaaga gatatagcaa ttatatctcc tataaacaat agtatattgg
     1921 agcatcaaat tagagattct ttgatagaaa aaaatataga tgtttttagt actaagaagg
     1981 ataaaaaagc tgtagattat ccatatggga atgcattggt agtggctaca tgtatatttt
     2041 atggatattt agattttata aaagatgatg aatacttaag ctttttagaa actcttttag
     2101 aaataaatag gataaaagca tttaagattt ttaatgaaac aagacattta agtattgata
     2161 atatagatga acatagtcag tatcgtgata ttttacagta tattgaagaa aaaaagaaga
     2221 gtgatataaa aatacatgaa tttttaactc aattttacat agataaaatg ttaaatttaa
     2281 aagaaggtaa aaagaatgtt gggttatgta aaaagataat agctgaaagt gagtctttta
     2341 gtgaaagttt aagtctactt aggatgaaaa aagaaaagat atttatagaa gctttaaaaa
     2401 gtacaataaa cgattattac tctgttgtcg atatagaaga gttgaaatcc aaagacaaaa
     2461 tagtaataac tactccttat tcatatatat catcaaatat agatagaagt atacaaatat
     2521 gggtagatat aggaagtaat gcttggaata tgaaaataga aaaagatata agtaatttaa
     2581 ttgtacttag aaaatctttt gaagaaaaaa agatttatac aaatgaaatg gaagaatatt
     2641 ataaaaaata ttatctatac aatacagtat ataatttgtt attaaatgca aagaaaatct
     2701 atgcatttaa aagtgaatat gcgattaatg gttatatcca agagagtata ttatatagta
     2761 tacttttaaa aatctcacat aaaggagata aaaattatga ttaatataaa ttatagagaa
     2821 gaccagatac caataataaa ttacgataat ggtactatgg cagtaccagc agtaccaggt
     2881 gctggaaaaa catttataat aacaaattta gtagctaaac tattacttga acagaaacat
     2941 aagggtggta aaatattaat tcttacatat atgaatagtg ctgtaaataa ttttaaaggt
     3001 agaataagaa agattcttga agagaataaa atagatgata ctaattcgta tgaagtaatg
     3061 actatacaca gtttggctgt aaaaataata aaggaaaagc cagaagttgt aatgttaagt
     3121 gaggatttta acatagctga cgatttgcaa aaaagtatta tattaaatga atgtataagt
     3181 agatttagat ttgaaggtgg agaaagagct tttagatggt ttttaaagga gcaaaaagat
     3241 gaacgctgga aagaaatagc tcttgaagcc tgggaaagag gtttttttga atttgcagga
     3301 aatgcaataa gtgaacttaa gtataaggga atttctccag ataaacttga agaaatattg
     3361 tcaagaggac acaaaggaat gcttaaaatt attttaccaa tatataaatt gtatgataga
     3421 aaattaaagc aaaatggatt gctagattat gatgatatat taattttagc agaaaaaact
     3481 ttaagcttag acgaagggct tagaaagaag tttcaaacta gatacaaata tatatttgaa
     3541 gatgagtgtc aggactcaaa tgaaattcaa ggtaatataa taaaaattat atctagtgaa
     3601 aataaaaacc tagttagagt tggagatata aatcagagta taacaggaac attttcatca
     3661 tcagacccta aattttttaa agaatttata aatagtgcag atttctgtta taggatggat
     3721 atgtcaaatc gaagttcaaa agatatactt gatttagcaa acactcttgt caagtatgtt
     3781 acaaaggaat ttagacaaga agaatgtaga catgctcttg aagatatgca aataaaaact
     3841 gtgccaaatg gaatgggata taaagaaaat cctaatccag aacattataa tataaatgta
     3901 aagtggtatg agagttggaa aattgaaata gagcaaactg ctagatacgt taagggtata
     3961 aaaaataaat atccagataa aagtataggt atattggttc catttaatga acaagtgaca
     4021 caagttgcta aagaacttag agagaataat ctaacctttg aagaattagg accaaattct
     4081 ttgaataaaa ggaaggttat aaacaacata gcatatatta ttgattttat gttacattgt
     4141 gatgatgtag aaaagttaat agttgtactt aataaagttt ttatagatac tgataatgaa
     4201 gaagaaataa agtatttttt agaagaatta aaaaactatt ctaccgaaga aattatttat
     4261 aattttgaat ttagtaagtt agataatgat ttaatagaat ttaaagaaga aagtgacata
     4321 tatttaagtt ttaaaagagg aatagatatt ataaaacaga tattagaata tccaataata
     4381 aggttagacc tcttagtttt gtttattggg aaaaaactta atttagaaaa agaagataaa
     4441 gctgtactgg attatatatc tttttatata aagtatctaa tatcagaaaa tataaatatg
     4501 gatttagaag atgtatataa tttattgttt gatgtaaaga atagggtgtt taatcatata
     4561 atagatgtgg tatatgagat aaatggatat gaaccagagc ccggtagtat aacactatgt
     4621 aactatcata agtcaaaggg gatggaatgg gattgtgtgt ttttactagg tcttgttgaa
     4681 tataatttcc ctgacaatat taatcaaaag tttcagagtg ataaatggta tctaaaagaa
     4741 aaatataaaa accctatggc aattattaaa tcagaagttg aagctatatt aaaaggaagt
     4801 atttcaacag attatgctca taaaacgaag attgattcta taaatgaaaa gattagactt
     4861 ttatatgtag ggattactag agcaaaggaa atgttagtac tttcaggaag tgcttataga
     4921 gatgaaagtg atatagggaa taagagaaaa gagcaaaaac catgtatata tttaagcaga
     4981 cttaatcaac atattattga aaaaaggtca aattaatata taataagtta ttaaaagatt
     5041 gcttgaaata tatctaaaag gggataaaac aatatgtaca gtaagtaact actaaaaaat
     5101 aaagttaaga gagaagtgat atgagtgaac gataaattaa aatatttttc ttatagtcaa
     5161 aattcaatca acacatataa atcatgtcca ttgaaattta aatataaata tattgataga
     5221 ataaattgga aaaatgatga tgttggtagt agagagtatt atgaaacttt aaaaacagga
     5281 agagattttc atttgatttg tgaaagatat tttagcaata tacctcttgg aatctatttt
     5341 aatgaggatg ataaaaatag taaaaagttt ttaaagtgga tggaaaatat aaaaaaagtt
     5401 gttcctattg aaaaaggaaa aacgtatttg ccagaatatg aagtaaggat gaccttaaat
     5461 ggggatataa tacaggcaaa gtatgattta gttgtaattg gaaataacaa tatagaaata
     5521 tgggattgga aaaccgagtc taaaaagatt gactataaac acgtagaaaa tagaatacaa
     5581 actattgttt atatgttttt agcaaaagaa gtaataccta aagttttaaa aatggacata
     5641 aatgttaaag atataaatat gagatattat caacctgaat ttgatgactt accaattacc
     5701 attttatatg atgaagaaaa acatgaagca aatagaaata aaattcaaaa ttatataagt
     5761 atgataaaaa acactaatta tgaagaacat acatacgaag acaatctata caatgatata
     5821 gaaaaggtat ataataaaga agaacgtatg tgctatagaa ataaaaagca ttgcaagtat
     5881 tgtgaattta ataagttatg taatggcaaa gaaatagatt atagtatttt ggaggcggaa
     5941 atttatggga cttaggtttg tattaggtag aagtggaagt ggaaaatcca cctatatact
     6001 agatgagatt aaaaaagaag ctcaaaaaaa tgaaacgaca tcaattatac ttttagttcc
     6061 agaacagtac acatttgagg ctgaaaatag agtaagtaag ctattcttag gaaaagaaaa
     6121 agataagtat ctaagagtta gggtacttag ctttaagaca ctaagtaata tagttttttc
     6181 tcaagtagga ggattgacag atgtaaatat aaattctagt ggaaaagcaa tgatggtata
     6241 tagagctatt gaagatgtaa gtgaagaatt aaatgtgttt tcaaaatcaa aatcgcagtc
     6301 tggttttgta agctcaatta cagatatgat atcagaaatg aaacaataca atataagccc
     6361 tgaaatgtta gaaaatatat ctggagaatt ggataatgaa actttaagtt taaaattaaa
     6421 agatataagt aaaatatata attcttttga agggaaattg catgaaaatt atgtagatgc
     6481 tcaagatatg ttaacttcat tggcaagtaa aattgaatta agcagttatt tagatggagc
     6541 ttgtgtttat atagatgaat ttacaggttt tacaccaaat cagtacaatg tcataaagag
     6601 tatattaaat aagtctaaaa gtgttaatat ctctttgact gtagatgata tcaactatat
     6661 tgggtacagt aaatctgata tgttttctag aacgaaattt acttattcaa aattgactca
     6721 attgtgcaac gaagaaggca taaaaatatt gccacaagta aatttaaaca caggtgttat
     6781 aaaaagattt gaaaaagtta aggaattaca gcacctagaa agattttata atgcttatcc
     6841 atataaaata tattcaaatc caactgaaaa tataaagata aaagagttta acaatttata
     6901 ttcagaggtg gaagagatag ccagagaaat tgtacattta gttagagata agaatgtaag
     6961 atatagagat ataaccattg ctacaagaga tttaaataga tatgattttt tagtacactc
     7021 tatatttaat gaatataata taccaaactt tatagataag aagagagagg caaaaagtaa
     7081 tccaatagtt attttaatta tttcagcttt agaaatgaag aatagacgat atgggtatga
     7141 aactatgttt aggtatttaa aaagtggact tataggaatt gataatgatg atataaattt
     7201 actagaaaat tatgtgttgg ctaatgggat taaaggtaaa aaatggtttg atgaaaaatg
     7261 ggattataga ataacacaaa gtctttcagg tcaagaaagt gaatttgaac tggaattaaa
     7321 agaaaaaata aatgaaataa aaaatagagt attagagcct atagtaattt tacaagaaaa
     7381 attaagagga aaaaatagag taaaagaaat ctgtagatat atatatgaat ttttattaga
     7441 tataaatatg ccagaaacta tagaaaattt aatagtaaat tttaaggata aaggggaact
     7501 tgatgttgca aatcaatact cacaagtttg ggacatagta gtggacatat tagaccaaat
     7561 ggtggaactt atgggtgatg aaataatttc tttagaaaaa tttataaaat taataacctt
     7621 aggctttgat gaatatgaat taggactggt tcctcctagt atagaccagg tacttgtaag
     7681 tagtgtggat agaatgaaaa atccagacac gaaatattta tacctagttg gaactactga
     7741 tggtgtgttt ccactgatta caaaggatag tggaatattg agtgataatg atagggaaag
     7801 tcttggaaac aaaggaatcg aagttgatat tgatagtaag actaggagtt ttgaagaaca
     7861 gtttcttgta tataaagctc ttacttcgac atctaagaat ttaaccataa catatccaat
     7921 atcagaccat gaaggtaaaa cgcttagacc atcaataatt atatcaagac ttaagaagat
     7981 atttccaaat atagaaaata aaagttattt agtggaagaa aataaaagca ctgataaaga
     8041 tatacttaaa aaaataacgg ttaaatcccc tacatttaat gaacttataa acgtaataaa
     8101 aaattatgac agtgatgatt acaatacaga agagattaat agtatttggt tagatatata
     8161 taggtactat ttaaaagatg aaatctatag ttctataaca aaaaaagtta taaaaggtct
     8221 tagttatact aatcaagttc ataagattga agagaaaaaa ataaggtcat tgtataaaag
     8281 taattctctt agcatatcta ggcttgaaaa atatgctgaa tgtccatttg cttattttat
     8341 acaatatggt ctaaaagcaa aaaagcgtaa agagtattca tttacaccac cagatttagg
     8401 aacttttatt cataatattt tagataggtt ttcaaaagaa cttttacaag ataatttaac
     8461 ttggagagat attgatgaaa aatatataga attaaagata ggtataatag ttgatgaaat
     8521 aattttaaag ataccaggat atatattaaa tagttctgag aggtataaat accttgcata
     8581 tagattaaag aatatgttga caactgctat aactataatt agtcagcaaa taaaacaagg
     8641 ttcatttgaa ccaatagatt atgaagttaa atttggcgac aatggaaaat atccacctat
     8701 aaaaatggtg ttagaaaatg gacaagaagt aagtttaatt ggacaaatag atagagttga
     8761 tgaatttgaa gaaggagaaa ataaatatat aagaataata gattataagt caggcaataa
     8821 gtccataagt ctaacagaaa tatattatgg tctacaatta caattacttg tgtatttaga
     8881 tgcaatcctt gaaagtgcaa aggatgaaga tataaatata aatccagctg caatactata
     8941 ttgtagaata aataatccaa tagcaaaatt taatgaagat aaagatgatg aagaaataca
     9001 agaagcaata ttaaaagaac ttagaatgaa ggggcttgta gtaaaagact ctcacatagt
     9061 taaagagatg gataaatcat taatagatgg agaaaggaaa aactctttag taataccagt
     9121 aggtcttact aaggatggaa atgtaggtaa aagtacttca gcaataagct atgaggattt
     9181 caaactgctt agaaaatatg taaggcatgc tataaaggat ttatgtgaag aaatgttaag
     9241 tggagaaata cgtatagctc catataaaca taaagatgga acatcatgtg acttttgtga
     9301 ttactctact atatgtcagt ttgattcaac tataaaagat aacaagtata aaaatttaaa
     9361 taataagagt aatgaagaga taataaaaat gatgaaagga gatgttaatt agtgagttct
     9421 cctaaatgga caaaagagca attagaagtt ataggaagta gagaatgcaa tttattggtt
     9481 gctgctgctg ctggttcagg aaaaacagcc gttctagttg agcgtataat tcaaatgata
     9541 acaagtagag aaaatccaat tgatatagat aagttattag ttgttacatt tactaatgct
     9601 gctgcgtcag aaatgagaga gaggataggg gatgctatag gaaaagcctt agatgaaaac
     9661 ccagaaaata aacatttaca aaaccaactt gttttattaa ataaatctag tattacaaca
     9721 atacactcat tctgtttgga tgttataaaa tcaaactttc atagaataaa cttagaccct
     9781 aatttcagaa taggagacca aactgaatgt gctattttga aacaagaagc aatagaggaa
     9841 gtatttgaag atttatatga agaaagagat gaggggtttt taaatttagt tgaaagttat
     9901 gcagaaagag gaggagataa agaagtacaa gatattatct tgggaatata ttcatttgca
     9961 atggcttctc ctgaacctaa aaaatggttg attgactcag cagaacgatt taatattgat
    10021 gaaaattttg atttttcaca gtctatttgg gctagagcta tattagacac tgtaaaaata
    10081 gaaattaatg gtctttgtct aaatatggaa agggcattaa aagaagtgga atcaatagaa
    10141 gaattagaaa cttttgcaga aaagttatcc gttgaatata aaaaaattgc taacatttca
    10201 caagcatgta ataaatcttg ggatgaggca tataaaaaga tggcaagtat gtcctttgaa
    10261 aattatgtaa aaggagtaaa aagaatttca aaagatgctc cttcgtatat aaaagaatca
    10321 aaagaaaagg caaaaactat aagagataag actaagaagt ctttagaaag tatagtaagt
    10381 gctacattca ataaagataa tgattctatt agagaagaaa taaaatatct ttataacata
    10441 gttaaaccaa tatctagtgt tgtactgaga tttgaagagg aatatagcaa taagaaaaga
    10501 gaaaaaggaa taatagattt taacgatata gagcattttg cattaaatat acttacagat
    10561 atagatgaaa aaggtaatat tgtaccttct gatatagccg ttggatatag aaacaagttt
    10621 tatgaaatat ttatagatga atatcaggat agtaatctag tgcaggaagt actgcttaaa
    10681 gctgttgcaa atacagaaac tccaaataga tttatggttg gtgatgtaaa acagagtata
    10741 tatagattta gacaagcaaa gccagaatta tttttgcaaa aatataataa ctataatgat
    10801 aaaaagggaa gttcccatag aaaaataatg ctttacaaaa attttagaag tagagaagaa
    10861 gttgtagatg ctgtaaatta tatatttgaa aatataatga atgagaatat aggagaaatt
    10921 gaatatacag aaaaagaaag actaaattta ggtgcgaatt ttaatgtgga tacagacgag
    10981 aaatctatta ttggtggagc aacggagata cacctaatac aaaaagataa taaacttgat
    11041 gatgatatta taaatgataa agacgataga attaataaca aagaaaatga aatagaggaa
    11101 gaagaaaaat tagataatat acaacttgag gctagaatgg ttggaaacat tatcaaagat
    11161 ttaatgaaag tcaatgaaga tggaaaaatt caaaaggtct atgataaggg aatagatgga
    11221 tatagacctg tggaatttag agatatagtt attcttttaa gagctacatc tgcatgggct
    11281 ccagtgtttg cagatgaatt gatgaatatg gatataccaa catatgctga tgttggagtg
    11341 gggtattttg atacgattga aataaaaaca atattgtcat tacttcaaat aatagataat
    11401 ccaatgcaag atatacctct tatatctgta ttaaaatcac ctatatttgg atttacacca
    11461 gaggatttga ttgatattag agttcagagt aaagataaaa ttttttatga ggttttaaaa
    11521 agtacagcag agtatgatgg atttacagat tctcaaaatg aaactgagtc tgagtttata
    11581 ccaagtgaag agtgcataaa caaaagtaaa gattttttaa ttaagctaaa ggaatttaaa
    11641 gaaaagtcaa tgtacatgag tacagatgaa tttatatggt acttgtacac aaggactgga
    11701 tactatgctt atgttggagc attgccaggt ggaagtcaaa gacaagctaa tttaaaagta
    11761 ttgtttgaga gagcaaaaca atttgaagaa accagtctta aaggaatatt caactttgta
    11821 aattttatag agaaattaaa aaaatctagt tctgatatgg gaagtgcaaa aacacttggt
    11881 gagaatgcaa atgttgttag aataatgagt attcataaaa gtaaaggtct agaatttcca
    11941 gtagtcatat gctctgcaat gggtaaaaat ttcaatactc aagattttaa aaagagtatt
    12001 ttatatcatc ataatttagg gtatggacct caatttgtag actatgagag aaggatttct
    12061 tttccaagta tagccaaaga agcattaaaa agcaaaataa atatagaaaa tttatcagaa
    12121 gagatgagag ttttatatgt tgcatttaca agagctaaag aaaaattgat tataactggt
    12181 tctactagaa atatacaaga tagtataaag agatggtcaa atggaattga aagtttagat
    12241 acaatatcac aatacgagat attaaaaggt aaaaattttt tagattggat aatgccttgt
    12301 gtattaagac atagggattt atctaatcta ctagaagaag taggattaga cgcagttttt
    12361 aatgttgaac ataattcaaa atggtatgga aagttgtgga ataagaatga tattttagtg
    12421 gaaaagaaga gtgatgaaga aaaagaaagt attgaagaaa ttcttgaaaa aatagatgta
    12481 gatagtccgg acagtgatta ttacagtgaa atagaaaaaa aattaaacta tatatatcca
    12541 tatgaattta gtacaagaaa acctgctact atatctgtta cagaaataaa aaagatacaa
    12601 aataattatg aagaagagtt aataaatact atatttgaac aaaaagtaat attaaaaaag
    12661 cctctattta ttcaaaatga agaagaaaga gaaaaaataa gtggaacaga aagaggtaca
    12721 atagtccatt tggttatgga ggttttagac ttaaaaaatg taagtagtgt aaatgatata
    12781 aaatcacaaa taagaggttt tgtttcaaaa ggtataataa cagaaaaaca agctagtata
    12841 gtaaatccat ataaaatata taaatttttt gcctctaaca taggaaaaag aatgttgaat
    12901 gctgaaatta taaatagaga aaaatcaatt tatgcacaag taaatatgaa agacatatat
    12961 atttatgaaa agcttataaa caatgatgat aaaaaacttt atgataatga aagtgtcatg
    13021 ttaagaggta tagttgatgc ttattttgaa gaagataatc aaatagtttt ggtggattat
    13081 aaaacagatt ttgtaaatga ggaaaatata aatcaaataa tagagaaata taaaaaacag
    13141 ttggatttat atgcagatat tattgaaact ttaactggta agtcagtaaa agagaaatgt
    13201 atatatttat ttggagttga tgaagctgtt tgttattagt ttttatatat aaattaaacc
    13261 taaaagatag aggaagggga aaaaatgaga tttattcata catcagattg gcatctaggg
    13321 aagtccctag aaggccattc tagaattgag gaacaagcaa agttttgtga ggaatttata
    13381 aaaatagtag aatcaaatga aatagatatg gttataattg ctggagatgt atatgatact
    13441 tctaatcccc cagcacaagc agaaaaacta ttttatcaga ctgtatcaag acttgccaat
    13501 aatggtcaga ggtgtgtatt aataatttca ggaaatcatg ataatccaga gagactttct
    13561 gcaataacac ctctagcaca tgaacagggg atacttatat atggttatcc attaagtgct
    13621 acgatagaag ctaagtataa agggtttgaa ataacatatg caactcaggg atgtacaaaa
    13681 ctgaatataa atggagaaaa tattgtgatt gctacattac cttatccaag tgaaaaaaga
    13741 ttaaatgaag ttttttcaag tgaagatgag tttgagaaac aaaaaaatta ttctgaaaag
    13801 gtaggagata tatttagaag tttagaagaa aattttagaa gtgatactat aaatatagcc
    13861 gtatcacata tatttgtaat aggtggagag agtactgaat cagaaagacc tatacaatta
    13921 ggtggaagtt ttttagttga aagaaaagat ttgccagaaa aggctcaata tactgcacta
    13981 ggtcatttac ataaacaaca aaaagcttcc gaacgtttaa atgcatacta ttctggttct
    14041 ccacttcaat atagcaagga tgaaagagcg tataccaaag gggcatacat agtggatata
    14101 aaagctggag aaaaaccaat aattgaagat gtatatttta ataattacaa acctatagaa
    14161 gtatttaaat gtaatggaat agaagaagca ctagatatat gtgagcaaaa tcaagataga
    14221 gagatttggt catattttga aataaatact gatgagataa tatcacagaa tgaaattaaa
    14281 aaaatgaagg aactattaaa agacataata gaaataaaac caataataac atcttgttat
    14341 gaacaagaaa gtgtagatat aaaagaaaag tctatggcag agttgtttag agagttctat
    14401 tcttttagta aaggtgtaga gccaaaagga gaacttatgg atttatttct agatataata
    14461 agtgaagaag gtgaatcagc agatgagacc aattagatta gaacttacag gacttaatag
    14521 ctatattgat aaacaagtta tagactttga aaaattaatt gaaagaggtt tgtttgggat
    14581 atttggaact actggaagtg gaaaatctac aatcttagat gcaataacta ttgctatgta
    14641 tggaaatata tccagaaata caaaagaata tataaacagt gtgtgcgata aggcaattat
    14701 atcatacgag tttgagatag gaagcaaaaa cactagaaga agatacattg tagatagaac
    14761 cattgctaga agcaaaacag gaacaaagac ctctaatgct agattaatag aagtattgaa
    14821 tgacaatatc caaaatgttt tagctgataa agttgtggaa gtaaatgaaa aagtagcaca
    14881 ggttgtagga ttaacggcaa atgattttac taggtcagta gttttaccac aagacaaatt
    14941 taatgagttt ctaagattat cacgtgcaga tagaagagac atgttagaga gaatttttaa
    15001 tcttgagaaa tatggaagaa gtcttggaga aaaagtgaaa aagagaaaaa atatacagtt
    15061 acaaaattta aaagatttga aatcaaaatt aagtcaatat gatggaatta cagaggaagt
    15121 atacaacaat ataaaccaag aattaataga gttaaaaaat ttggaaaaag ataagaataa
    15181 tgctttagac ctagctcaaa aatcttatga agaaagtaaa acagtatatg aagaacaatt
    15241 aaagttagaa aaaaatgaac ttagaaaaaa tgagcttgat ttaaaaaata gtgagataaa
    15301 agaaaagatt gctcttgtag aaaatgatag taacgctaaa aaggtagacc catacataag
    15361 ttcagttcaa aatctagaaa aaaagataga tgaagatagc tttgtagtaa gtaatttaga
    15421 aaagaaatta gcaatattaa atcaggagct tgaagttaca aaaaatagat atgaaaaaat
    15481 aaacaaaatt aagaatgaag aagtccctaa attgagtgaa gaaaaaataa gattgcaaca
    15541 agcaataaaa ttagaagaag agttagtttt attagataga gagttaaaag atctaaaaga
    15601 aagtggaatt aatttaaata agactaaagt tgaattagaa aaagtaaagc aggtgtcaga
    15661 gtccagaaaa gatgcagtta ctaaatctat aaaagaagtt gaaggaaaga ttgataaagt
    15721 aaatataagt gcagaattaa aacaaaaaat atttctagca tatgaatacg agaaagatta
    15781 caacaacgta ttggaagaaa aaaatcaaaa gctaaataaa ctagaagaaa tactcaaaga
    15841 gacagaaaat ataaatttaa aagttagata tattgataaa gataagaatg atgtaaatcg
    15901 taatttagaa aatttatcac ttcacttgga tgttcttctt aaaaaatgtc ctggtaagag
    15961 tgctgattta cttttaaaat ctgaatatgt tactgaatta agaagtaaag caaataatac
    16021 taaagaaaat gaaattaaga aaagcagtat acaagatgaa ttaaagataa tacttgaaag
    16081 taagtttaat actgaaaggg aagtaaattt actaaatgaa aaacttgaaa ataatagaaa
    16141 gaatagagat gacctagaaa aagaattaga agaactaaag tatttaaatt tagcctctga
    16201 acttagacgt gaattaaaag aaaatatgcc ttgtccagtt tgtgggtcaa aacatcatga
    16261 aaatcataat ataactaagt atgatgaaaa tatatctttt gtcaaagaaa aactggagaa
    16321 attagaaaaa gaaaaaataa gtataagaaa caatatagag gaattaaatg ctaaagtaag
    16381 tgggtattta tctatagaaa aaatgaagac taaagaactt gaagatgtaa aaggaaaact
    16441 aggggggatt ccatctagtc aattattaaa aaaacttgat gaagaacaaa gaaaacttgc
    16501 attgttaaaa tctaatattc aggagtggga gaaagaaaaa gagagtactg aaaataaagt
    16561 aacattagct aaagaagaaa aaaataaaat tgaaaaagaa gaattaaaaa taagagagag
    16621 tctgaataat tataagaaat taacaagaga tttaaacata gaaatagaag gtttagaaaa
    16681 taaatgtaaa aaacttaaac aggaatacct aggattaaag acgattacta aagtctctaa
    16741 tctattgtca aaagtaaaag aaataaggga gaatgaaaaa gaattagaat tactaagtac
    16801 tgggcattca aatttattaa aaaatagaga ctcactagat attaaaataa gagaaaatga
    16861 gagtaaacta catgaaatag agatagaatt aataaaggct agagaattat atgtagaaaa
    16921 gaaattgagt agagataata agtataaaga agttattaac ataacgaaag gtgatttagc
    16981 taaaaatctt ttacataatg ttgaagaaaa tatatgtaag atattagaac aagaagaatc
    17041 aagtaagaaa aaattggaag agcaaagatt agaatatgaa aaaaacattg ctgaaaagca
    17101 caatatagat ggtagactta aaactgctaa ggaacaatat aaagaccaaa aatcaacact
    17161 aaataaacta ttagcagaaa ataaatttga aagtatatat gcagtaaaaa gagctttatt
    17221 agatactgat gcagtaaaaa aattgattga agaaatacta gagcatgaag aagaacaaaa
    17281 gttattatca tttaagataa aatcatcaaa agaaaaatta aatggaagaa gcataaagaa
    17341 ggagtacttt gaacagttaa aagatgaaat ttataatttg aaagtagaaa taggaaatat
    17401 atccaaagat attggagcta atcaaaatca gttaataaca ctaaaagact ctttagataa
    17461 gataaatgat tttaataaac aacttaaggt agttgaacac aatgtggact tattggaaga
    17521 attagataaa tgtgtgcaag gaaataaatt tgtagaatat gtttctacaa accaattaaa
    17581 atatatagca ttggaagcat caaaacgatt ggatggaata acaaaaggaa gatatgcact
    17641 tgaaattgac tcaacactaa actttgtaat gagagataac tttaatggag gagaaagaag
    17701 aagcattgat actctatctg gtggagagac attcttgaca tctctatcat tagcacttgc
    17761 tctttcatct caaatacaat taaagggaag tgcaccatta gaatttttct ttttagatga
    17821 aggttttggt tcattagata gtgaactttt agaaattgtt atagaatcct tggaaagatt
    17881 gcatagtaat aatctaagtg tgggtataat aagtcatgtc gaggaactta aaaatagagt
    17941 tcctgttaag ctattagttt catctagtga ggctggaata ggttcaaaag taaaaattga
    18001 atatagttag aaaatatatg gtaattgaat atattatcta atatttccaa ataatatgta
    18061 attgtgatat aataaaaact aatattaaag ttatttataa ttaatattaa tataaaaaca
    18121 tgtaagtagg agtgtggtaa gtgttggaat cgcccaataa tttgattcag attatttttt
    18181 taatagtatt acttataggg tctgcatttt tctcagcatc tgaaacggcc ttaatgtcgt
    18241 tgagtaaaat cagaatcaga tatatgcaag atgaaggagt taaaggagct aagttagtaa
    18301 gttcattaat agaaaatcca aataagcttt taagttctat attggttgga aataacgtcg
    18361 taaatatagc agcaacatcc atatcaacat ctttgtttat aggattaatg ggagaaaaag
    18421 gagtggcact tgctacagct gttatgacag tattagtatt aatatttggt gagattactc
    18481 caaaaacaat agcagcaaat aattcagaaa aggtttctct attagtatct aaacctataa
    18541 aagcaattat atttatatta agaccaatag tatggatatt taatataatt acaaatatta
    18601 tatttaaatt atttggtata acaaataaag gagctaaatc attcataact gaagaagagc
    18661 taaaaactat ggtaaatgta agtcatgaag aaggcgtcct tgaaatggaa gagagagaaa
    18721 tcataaataa tgtttttgaa tttggagata tgcaagctaa aaatgcaatg gtacaaagaa
    18781 tagatatggt agctatagat atggaagata gttatgatga gataatacag gtatttaaaa
    18841 ctgaaaaatt aagtagaatg cctgtgtatg aagaaactat agatgatatt gtaggaatac
    18901 ttaatataaa agatattata tttttatctg atgaagaaat agagtcattt gatattaaaa
    18961 attatatgag agaacctttt ttcacgtatg agttcaaaaa gataacacaa cttcttgaag
    19021 agatgaaact tgaaaaaagt cagatggcta tagttgtaga tgagtatggt ggaacatctg
    19081 gacttttaac tatagaagat ttagtagaag tcattgttgg agatatagag gatgaatatg
    19141 atgaagaaga ggatgaaata caagttataa aagaagatga gtatattgta gatggaagta
    19201 ctaaaattgg tgatgttaat gaacttattg gtgtaaactt ggaatctgag gagtttgatt
    19261 ccataggagg ttttataatt ggacacctaa gcagattgcc agaagaaaat gaagtaattg
    19321 aagttgataa tataagattt tgtatagaaa gtatagaaaa aaatagaatt aaaaaaataa
    19381 gaatatatac ataatgtaat aatgttctta aatttattag aagtgtataa atataaaaga
    19441 tatcttttat atttatacac ttctttttat tttgtattca aataaaacta gtatgaaatg
    19501 gacaaaataa aaatataaat tagaagaaag gagattttta agatgagcat actagaaaat
    19561 aattttttat acaaattaaa aaacaacact atcttttttt gtatatatac attgttattt
    19621 atttttattt acaaagcgtt tccatacata gctccatttt ttctaggtgc tataatagca
    19681 cttatgatta atcctatatc acaaaagtta gaaaataagt ttcgtataaa taaaggtatc
    19741 tctactcttg ttctaagttt tcttgcagtg gctatagtca gtacagttac tacaataata
    19801 gtaataaatt caatgaaaga attaatggga tttttaaata atatatctgc taatcctgag
    19861 gatataagta atactattat gtatttttta aataaaataa atgactttat gaaatcattt
    19921 caagaaatag ccaattttga tgtagaacaa ttagtaaaca aattcagtgg tgaagtaatg
    19981 caaattacaa aaaatcttct tactagtata cttggattgg ctacttctat tccatatatt
    20041 ataatattta taattacctt gtttattgct acctacttta tagcaaaaga ccttgataag
    20101 attgaaaata gtttttataa tatgtttact gttgatgtta gaaagaaggt aaaaaacgta
    20161 aagaaagagg cagggctttc acttgttgga tatataaggg cctatacaat acttatggct
    20221 ataacatttt ttgcaatctg gggaagtttt gctttatttg gattgaaata cggtctaata
    20281 gtagggtttg tagggcttta atggatttaa ttccattcct aggtataata acaatatatc
    20341 ttccagtaat tgtttattat tttcttataa aaaattattt tatagctata agtatgactg
    20401 tgatattttt tgttttgtct ttgattagag aaatacttga acctaaacta gtatcagtaa
    20461 atgttggact aaatccatta gctaccctag cagcgatttt tattggaatc caagtaaaag
    20521 gaataattgg agtaattttt tgtttgggtt tggtatgtat gcatgatata cttaagaagg
    20581 tggatatttt ttaattataa taattgatag atttttttaa aaataaaatt ttattcacag
    20641 gtaaataaaa aaatattaat cttattaaga aagagggtat atacgtgaaa caaaaagtag
    20701 tggaaagatt tttaaaatat gtatcatttg atacaacatc taattctcag tgtgagaact
    20761 gtccatcttc agaaggtcaa agagttttag ctaagtatat agttgaagag ctaaaaacaa
    20821 tgggtgttga tgatgtgagt ttagatgaaa atagttatat aatggctact ttaaaaggaa
    20881 atactgatgg tgtggatact ataggattca tatcacattt agatacaatt gaagatgtta
    20941 gtggaaaaga tattaaacca agaataatag aaaattatga cggaaaagac atagttttaa
    21001 atgaagcgtt aaatgttatt acgtatgtaa aggattctcc agagttggaa gaatttaaag
    21061 gtgacgattt aatagtcact gatggaacaa ctctacttgg ctctgatgat aaagctggta
    21121 tagctgagat agtgactgca atagagtatc taataaatca tccagaaatt aagcatggag
    21181 atataaaaat aggatttaca ccagatgaag aaattggtag aggtgctgat ttatttgatg
    21241 ttgaaaaatt tggagcaaaa tatgcatata ctttagatgg aggaatagtt ggagaacttg
    21301 aatgtgaaaa ttttaatgct gcaaatgcaa ctattactat acatggtaga aatgttcatc
    21361 caggttcagc taaaaataaa atggtaaatg ctatacatat agctgctgaa atatcagaaa
    21421 tgttcccagc agatgaaaga ccagaaacaa cagaaggata tgaaggattt tggcatttaa
    21481 attctatagg tggaaatgtc gagaatgtaa gtatggctta tattattaga gaccattgta
    21541 gagaaaaatt tgaaaataga aaatctatta tgatagagaa tatagaaaaa ataaataaga
    21601 aatacgataa tagagtggaa ttggatttaa aagattctta ttacaatatg aaagaaaaga
    21661 ttgagccagt tatgtttata gttgatatag ctaaagaagc tatggaggaa ttaggtataa
    21721 aaccacgttt agttcctgta agaggtggta ctgatggagc aagattatct tttaatggat
    21781 taccttgtcc aaatatattt actggtggat taaacttcca tgggaaaaat gagtgtatac
    21841 cagttagttc tatggagaaa gctactaaac taattgtaag aatagctgaa aaatatgctg
    21901 aaagagttta gttaaaaagt atttaaaaat taagcattta aagttataat agctatatca
    21961 ttctttataa gtcaagctga ttataagctt gtgttgtatt taaaatggta tagctatttt
    22021 tgtaattatt tttataaaag ataattattt ttagtttagg gtatcaataa aaagtatgga
    22081 tatcaatagg tattaacatt taaaaggggg aattgtattg aaaattaaat ctaaaaaaat
    22141 aatagcattt gcactttcac ttgggattat gttgccaagt tgtgtatatg cagacactat
    22201 tagtaaaaat caaaatgcac aggtagatat gaacaattat cttcaaggta gaaaatctcc
    22261 aaaggaggaa aaacttattg gtgaagatag atttgataca gcgataaaaa tatctcaaag
    22321 tggttggaat aatggttcag aaagggtgtt tctagttaac tcaaattcat tgccagatgc
    22381 attggcttca actcctttgg caagtaaatt ggatgcacca atactgctta caaataaaaa
    22441 tagtatacca tataatgtaa cagaggaaat aaaaaggctt aatccagcag aagttatttt
    22501 aattggaagt gaaggagcta tttcatcaag tgtaaaagaa tctcttgaag atatggaagt
    22561 attagtcagt agaatcggtg gagcagatag agaagaaaca tctttacttc ttactagaca
    22621 gctagatgat actggtgatt taggtgtctc aaaagtggca gtggttaatg ggtataatgg
    22681 actggcagat gcaacttcaa tatcaagccc agctgcaagt gataatacag ctataatata
    22741 tacaggtaag gatagcataa gaagtgaggc aaagaatttt attacacaaa actcaactaa
    22801 gacatacata gtaggtgggg agtacagtat aagtaaaaaa cttgaaggac agttagtaaa
    22861 ctcagaaaga ctagcaggaa cagatagaaa agatactaat gcgaaagttt tagaaaagtt
    22921 ttatggaaaa tcaagtaagg ttaataatat gtattttgca aaagatggaa gtggtagaga
    22981 agctgattta gttgatgggc ttgctgctgg agtattagca tcaaaaacaa agtcacctgt
    23041 agtacttgca agtggaagtt taagtagtgc acagaaaagt tttataaaaa aagtaaaggc
    23101 tgataaattt gtacaagttg gtggaggtaa aaatagtaaa ccatatgcag aagcattagc
    23161 cttacagtaa tattgtgtaa atcaaaatta atatataata atagcaagta aaccaagttg
    23221 aacttcaata tatataataa aataagtaaa ctaagttata tttcagtata atatataatg
    23281 aaataagtaa actaagttat actccaatat aatatataat agaaacaagt aaactaagtt
    23341 atatttcaat atagcatata ataaaacaag taaaccagat tctatttaat tagaagttta
    23401 aggtattgta cttaaacttc taattgagat agaaataaaa tggtttactt gtttttatta
    23461 tgagtttaat aaattaaagc ttattttatt tttattaaaa atttactaat cattgatatt
    23521 tattatattt ccagtattga tgtcaattaa gtttatatca agttcaagct catcctcatc
    23581 atcagttttt atgatatcta taattgcgac agaatggctt ccatcttttg ggatagaagt
    23641 acttcctgga tttattacta ttaaattatc atccatgtat agttttttta catgagtatg
    23701 tcctaaaact aatatgtcac cacccatttt ctttgctttg tcaataattt cttcttctga
    23761 ttctatataa ccatgattca atataattct aatttcacca aattggctca tgacataagg
    23821 actctgaata ggatgttcta taaccatctg gtcaacatct gcatcacagt tcccttttac
    23881 aatcacaatg ttttctaatt tatttaatgt ctcaataaat tttttaggat tatatccttc
    23941 tggaatatca tttctaggtc catgatataa gacatctcca ccatgtaata aaacatcaca
    24001 atcagataat acatttaacg ctttttcaaa gtaaagtaag ctaccatgag tatcactcat
    24061 tattcctatt ttcatattaa aaatcctcct aagtacagtc tataaaaatt tttcagatac
    24121 tctatgccaa aactcatagt ttgacattct aagtaatttt atttttgcat cagatatact
    24181 aacttttata ttttcgattt gtctaaatct gtgctcaact ccatcaacga caatcaatac
    24241 agaatcttca aatctatatt ctggtgcaat ctttataaca gattcatttg aacatattat
    24301 acttgatgta aagcacctat atgcattggt atttatcgga tgtaaaggtg taatctgcat
    24361 aagttcaaga tttatatcaa caatactacc tccagcagaa tagttatagg ctgttgaacc
    24421 tgtggaagta gaaataatca taccatctcc actaaaattt tgtatatgtt tattatcaag
    24481 gcttaaattt aggtgtatag tcctagactt gtctcctctt ataacaacct catttacagc
    24541 aagcatatta gaaccactag tagttgtata tacttccgca ttaagaagtg acatttcttg
    24601 aataatataa tctttttttg tataagcttc aataaagcta tcaatcttat ctggtaaaat
    24661 atcaggaaaa aaccccagat gacctgtatt tattcccatt atgggaatct caggaaaatc
    24721 gaaatctcta actgttctta aaaaagagcc atcaccacca attgatataa taagctctgt
    24781 gtctggataa atatctgaat acacttcaaa gccagcattg ataagttttc tagttaagag
    24841 ttctttagtc tcaagggact tatttaattg gtttgtattt atagttataa ttcttttcat
    24901 atattcacct cttcatactt tgtattataa catagtttta taattcttaa tttataaatt
    24961 tataaatgac ctttgtagtg aatgtgttaa aatatgtata ggtgggtata aaaaaatgca
    25021 taagaggtga agatgtgttt agcataagga atatcacaaa aaaattagga aaattcaagt
    25081 taaataatat aaacttagag ttaaaggaag gggatattgt tgggattata ggaccaaatg
    25141 gttcgggaaa aacgacttta ataaaaatta taatggggat tattgatgca gatgaaggtg
    25201 aaattgaact ttgtaatgaa actattgaaa attctccaat aagctttaaa aacaatattg
    25261 gttttgtata tgattcttta caattttatc cacatttaaa ggttaaggaa tttagaaaaa
    25321 tagtttcttt attttataag aattttgata gagaaaaatt tgatgaatac cttaataagt
    25381 ttgacataga agaaaatatg catattgaaa atttatctaa aggtcaatct gagaagctta
    25441 tgttatcaag cgcacttagt cataatgcta agttattgat tttggatgaa ccaactgctg
    25501 gtatagaccc gattgtaaga actgaaataa tgcagtactt acaagatttt gtgaaaaatg
    25561 gaagtagtag tgtaataatt tctactcata atactgataa tttaattaaa attgctgatt
    25621 atcttgtttt tataaataga ggaaatcaga tttttactgt taagaaagaa ttaatagaac
    25681 aagaatataa gataattagg gcaaataaag cagagttaga agcaattaaa gaaagtatag
    25741 ttggtgttaa agagtataaa tattataatg aggcacttgt taaagttgga gatagcttga
    25801 aagtaaaaag cctattgata gaaattgata agcataaggt caaaaatcct actatagaag
    25861 aactaatgta ttattatgta aatgaggtga gaggatgttc aatttaataa gaaaagattt
    25921 aataatagga attagttcag atggtatccg taatttaaag tacatattgc tattttttgt
    25981 gttttatttt ttccttaatt caatttcata ttatacagta agtattgttg ttagttattt
    26041 gatatttata aatacatttg agtgtgatta tgaaaatgat tcaaggattt ttataaggag
    26101 tatgccagtt agtatagaag atatagttta ctcaaaatat ttattaggtg taggactaat
    26161 aatttctgta acaataattg tgtccttact aagtaaattg acatcactag tgttctttag
    26221 aaatatggtt ttaaatgatg tatttttttc tgtaaatata tttttagcaa ttttgacgat
    26281 tttactgcct ttaattttta agtttggata tggaaaaatg aaagtttgtg gttttatagt
    26341 aagtatttta atttactttg tatatggaag tcttttgaag atgattagta tgattgtata
    26401 tcaagtaaag cattttaatt atagtaaagt tggaggagta tatctttcaa attatgtaac
    26461 tgatatagct aatactaagt atataaattt atatagtatg acatttttaa caataataat
    26521 ttttataata tcaatgtatt tttctattaa gatttcaaaa aagaataagt ttaattatta
    26581 aaattttaat taatattata gcacaatagc acaaaatata aggagactaa aaatgaagaa
    26641 gtttataaaa gtttcatttt ttattttggg gatttttaca ctattcatag ctataaataa
    26701 attagtatat agagatatgg taagtgtcaa agattatgaa ataaatcatt tagaggaaat
    26761 tgtttttgat tttgatagca ggagaagtag agatatcggt actattgtaa gatttaaaat
    26821 aagcaataat agtaaatata gttataaatt aaacagtgct aaaatgaagt ttgaaaattg
    26881 tgtggaaaat aaaaagggaa tgaataagtg ttctgcatat gtaaatgtag atttatttaa
    26941 tgaaaaaaat tctataactg aaagtactct gaaatatgga ataaaaccaa ataatcaagg
    27001 ttatgttgat tttgtaattc caaaaggact aaattttgat tataggtact ttaatgaatc
    27061 tggtatgagt gtagaatata aaggagaata tattgtcaat attccaatgg caaaaggact
    27121 ttatctagta gttggaaaaa ataatgatac atggaatgct aatattatgg aataatttga
    27181 aaaataaatc aattatagat ataattaaga ataatatgaa atttataaaa attcagacta
    27241 ggaggggtat ttaatgtcaa taagtaatgt aagagagtat tttaaacaat ttggcaagga
    27301 agacagtata ttagaatttg aacaatctag tgctactgtg gaacttgcag ctgaagcagc
    27361 tggggttatt ccagcaagaa tagctaagac actctcattt aaaataggag atgatgcaat
    27421 tttaattgtg acagctggtg atgctaagat tgataataag aagtataagg ctgaatttaa
    27481 ttgtaaggct aaaatgttga caccagaaga agtcttggag tttactggac atgctattgg
    27541 tggagtctgt ccatttggtt taaaaaattc tataaaagtc tatttagatg attcaatgaa
    27601 gagatttgat acagtatttc ctgcatgtgg cagtagtaac tctgcaatag aacttacatg
    27661 tgaagaaatg gagaagtttt ctaagagtga aaaatgggta gatgtttgta agaattggta
    27721 aaaaaatata gtaaactata gtaggaatta gtaaaaaact acctctgtat acaatctata
    27781 cagaggtagt tttttgtgta aatatttata aatctaagag gaaaatctaa tttaaactaa
    27841 taatccaaaa taaagttaaa aaattctgat tttttttcga aacaataaaa aaatgacaaa
    27901 aaagtggaaa aaatgtaata aatattgtta aaaaaataat tagaaattat tgaaaaatta
    27961 tcctttgagt gttactatta tagatgtaag atatttaaaa ataaaaaata gtcagataaa
    28021 aatacgatta tttagctcta ttaaaataat ttttatacga taaaaatctt ctgtacatgg
    28081 ctaaaaaaca gatgtacaga caatatttaa tgtttaatat accctaatat aaatatcgta
    28141 aacaaattat tttatctgtg accagtgtga aagaaatctt attctaactt gaaatttcac
    28201 aaaattccaa aaagcgagat tttttttaag tatagttgat tttaaattaa caatttatga
    28261 ttaaattaat gtcataattg gttagtataa tcacatataa atatagagcg tagtttgatt
    28321 aaggttataa atttctcgac gtatattcaa ataccttatt atagatgttg ccgagatttt
    28381 aattaataaa tgaaaataaa atatttataa aatactgagg aggctattat ggatttaaat
    28441 tctaaaaaat atcagatgct taaagagcta tatgtaagct tcgctgaaaa tgaagttaaa
    28501 cctttagcaa cagaacttga tgaagaagaa agatttcctt atgaaacagt ggaaaaaatg
    28561 gcaaaagcag gaatgatggg tataccatat ccaaaagaat atggtggaga aggtggagac
    28621 actgtaggat atataatggc agttgaagaa ttgtctagag tttgtggtac tacaggagtt
    28681 atattatcag ctcatacatc tcttggctca tggcctatat atcaatatgg taatgaagaa
    28741 caaaaacaaa aattcttaaa accactagca agtggagata aattaggagc atttggtctt
    28801 actgagccta atgctggtac agatgcgtct ggccaacaaa caactgctgt tttagacggg
    28861 gatgaataca tacttaatgg ctcaaaaata tttataacaa atgcaatagc tggtgacata
    28921 tatgtagtaa tggcaatgac tgataaatct aaggggaaca aaggaatatc agcatttata
    28981 gttgaaaaag gaactcctgg gtttagcttt ggagttaaag aaaagaaaat gggtataaga
    29041 ggttcagcta ctagtgaatt aatatttgag gattgcagaa tacctaaaga aaatttactt
    29101 ggaaaagaag gtcaaggatt taagatagca atgtctactc ttgatggtgg tagaattggt
    29161 atagcagcac aagctttagg tttagcacaa ggtgctcttg atgaaactgt taaatatgta
    29221 aaagaaagag tacaatttgg tagaccatta tcaaaattcc aaaataccca attccaatta
    29281 gctgatatgg aagttaaagt acaagcggct agacaccttg tatatcaagc agctataaat
    29341 aaagacttag gaaaacctta tggagtagaa gcagcaatgg caaaattatt tgcagctgaa
    29401 acagctatgg aagttactac gaaagctgta caacttcatg gaggatatgg atacactcgt
    29461 gactatccag tagaaagaat gatgagagat gctaagataa ctgaaatata tgaaggaact
    29521 agtgaagttc aaagaatggt tatttcagga aaattattaa aatagataaa tattaaaata
    29581 tacacaatag gtcgatatta aaatagatat ggaggaagga tttatgaata tagtcgtttg
    29641 tataaaacaa gttccagata caacagaagt taaactagat cctaatacag gtactttaat
    29701 tagagatgga gtaccaagta taataaaccc tgatgataaa gcaggtttag aagaagctat
    29761 aaaattaaaa gaagaaatgg gtgctcatgt aactgttata acaatgggac ctcctcaagc
    29821 agatatggct ttaaaagaag ctttagcaat gggtgcagat agaggtatat tattaacaga
    29881 tagagcattt gcaggtgctg atacttgggc aacttcatca gcattagcag gagcattaaa
    29941 aaatatagat tttgatatta taatagctgg aagacaggcg atagatggag atactgcaca
    30001 agttggacct caaatagctg aacatttaaa tcttccatca ataacatatg ctgaagaaat
    30061 aaaaactgaa ggtgaatatg tattagtaaa aagacaattt gaagattgtt gccatgactt
    30121 aaaagttaaa atgccatgcc ttataacaac tcttaaagat atgaacacac caagatacat
    30181 gaaagttgga agaatatatg atgctttcga aaatgatgta gtagaaacat ggactgtaaa
    30241 agatatagaa gttgaccctt ctaatttagg tcttaaaggt tctccaacta gtgtatttaa
    30301 atcatttaca aaatcagtta aaccagctgg tacaatatac aatgaagatg cgaaaacatc
    30361 agctggaatt atcatagata aattaaaaga gaagtatatc atataattta aggggggata
    30421 aagaatgggt aacgttttag tagtaataga acaaagagaa aatgtaattc aaactgtttc
    30481 tttagaatta ctaggaaagg ctacagaaat agcaaaagat tatgatacaa aagtttctgc
    30541 attactttta ggtagtaagg tagaaggttt aatagataca ttagcacact atggtgcaga
    30601 tgaggtaata gtagtagatg atgaagcttt agcagtgtat acaactgaac catatacaaa
    30661 agcagcttat gaagcaataa aagcagctga ccctatagtt gtattatttg gtgcaacttc
    30721 aataggtaga gatttagcgc ctagagtttc tgctagaata catacaggtc ttactgctga
    30781 ctgtacaggt cttgcagtag ctgaagatac aaaattatta ttaatgacaa gacctgcctt
    30841 tggtggaaat ataatggcaa caatagtttg taaagatttc agacctcaaa tgtctacagt
    30901 tagaccaggg gttatgaaga aaaatgaacc tgatgaaact aaagaagctg taattaaccg
    30961 tttcaaggta gaatttaatg atgctgataa attagttcaa gttgtacaag taataaaaga
    31021 agctaaaaaa caagttaaaa tagaagatgc taagatatta gtttctgctg gacgtggaat
    31081 gggtggaaaa gaaaacttag acatacttta tgaattagct gaaattatag gtggagaagt
    31141 ttctggttct cgtgccacta tagatgcagg ttggttagat aaagcaagac aagttggtca
    31201 aactggtaaa actgtaagac cagaccttta tatagcatgt ggtatatctg gagcaataca
    31261 acatatagct ggtatggaag atgctgagtt tatagttgct ataaataaaa atccagaagc
    31321 tccaatattt aaatatgctg atgttggtat agttggagat gttcataaag tgcttccaga
    31381 acttatcagt cagttaagtg ttgcaaaaga aaaaggtgaa gttttagcta actaatttct
    31441 tggggagggt attatgagta caagtgatgt taaagtttat gagaatgtag ctgttgaagt
    31501 agatggaaat atatgtacag tgaaaatgaa tagacctaaa gcccttaatg caataaattc
    31561 aaagacttta gaagaacttt atgaagtatt tgtagatatt aataatgatg aaactattga
    31621 tgttgtaata ttgacagggg aaggaaaggc atttgtagct ggagcagata ttgcatacat
    31681 gaaagattta gatgctgtag ctgctaaaga ttttagtatc ttaggagcaa aagcttttgg
    31741 agaaatagaa aatagtaaaa aagtagtgat agctgctgta aacggatttg ctttaggtgg
    31801 aggatgtgaa cttgcaatgg catgtgatat aagaattgca tctgctaaag ctaaatttgg
    31861 tcagccagaa gtaactcttg gaataactcc aggatatgga ggaactcaaa gacttacaag
    31921 attggttgga atggcaaaag caaaagaatt aatctttaca ggtcaagtta taaaagctga
    31981 tgaagctgaa aaaatagggc tagtaaatag agtcgttgag ccagacattt taatagaaga
    32041 agttgagaaa ttagctaaga taatagctaa aaatgctcag cttgcagtta gatactctaa
    32101 agaagcaata caacttggtg ctcaaactga tataaatact ggaatagata tagaatctaa
    32161 tttatttggt ctttgttttt caactaaaga ccaaaaagaa ggaatgtcag ctttcgttga
    32221 aaagagagaa gctaacttta taaaagggta aaaatctaat taaaaatata tttgctgtta
    32281 actagattaa atagtatttt aatctagtta acagaatata aatactaaaa taacaggagg
    32341 ttataactat gaaattagct gtaataggta gtggaactat gggaagtggt attgtacaaa
    32401 cttttgcaag ttgtggacat gatgtatgtt taaagagtag aactcaaggt gctatagata
    32461 aatgtttagc tttattagat aaaaatttaa ctaagttagt tactaaggga aaaatggatg
    32521 aagctacaaa agcagaaata ttaagtcatg ttagttcaac tactaattat gaagatttaa
    32581 aagatatgga tttaataata gaagcatctg tagaagacat gaatataaag aaagatgttt
    32641 tcaagttact agatgaatta tgtaaagaag atactatctt ggcaacaaat acttcatcat
    32701 tatctataac agaaatagct tcttctacta agagaccaga taaagttata ggaatgcatt
    32761 tctttaatcc agttcctatg atgaaattag ttgaagttat aagtggtcag ttaacatcaa
    32821 aagttacttt tgatacagta tttgaattat ctaagagtat caataaagta ccagtagatg
    32881 tatctgaatc tcctggattt gtagtaaata gaatacttat acctatgata aatgaagctg
    32941 ttggtatata tgcagatggt gttgcaagta aagaagaaat agatgaagct atgaaattag
    33001 gagcaaacca tccaatggga ccactagcat taggtgattt aatcggatta gatgttgttt
    33061 tagctataat gaacgtttta tatactgaat ttggagatac taaatataga cctcatccac
    33121 ttttagctaa aatggttaga gctaatcaat taggaagaaa aactaagata ggattctatg
    33181 attataataa ataagaattt gggaattaaa agtttaaata aaattgttta aaaaacaatt
    33241 tcggatatat gaaaaatcta atttaatggg ggtaatgaat atgagagaag tagtaattgc
    33301 cagtgcagct agaacagcag taggaagttt tggaggagca tttaaatcag tttcagcggt
    33361 agagttaggg gtaacagcag ctaaagaagc tataaaaaga gctaacataa ctccagatat
    33421 gatagatgaa tctcttttag ggggagtact tacagcaggt cttggacaaa atatagcaag
    33481 acaaatagca ttaggagcag gaataccagt agaaaaacca gctatgacta taaatatagt
    33541 ttgtggttct ggattaagat ctgtttcaat ggcatctcaa cttatagcat taggtgatgc
    33601 tgatataatg ttagttggtg gagctgaaaa tatgagtatg tctccttatt tagtaccaag
    33661 tgcgagatat ggtgcaagaa tgggtgatgc tgcttttgtt gattcaatga taaaagatgg
    33721 attatcagac atatttaata actatcacat gggtattact gctgaaaaca tagcagagca
    33781 atggaatata actagagaag aacaagatga attagctctt gcaagtcaaa ataaagctga
    33841 aaaagctcaa gctgaaggaa aatttgatga agaaatagtt cctgttgtta taaaaggaag
    33901 aaaaggtgac actgtagtag ataaagatga atatattaag cctggcacta caatggaaaa
    33961 acttgctaag ttaagacctg catttaaaaa agatggaaca gttactgctg gtaatgcatc
    34021 aggaataaat gatggtgctg ctatgctagt agtaatggct aaagaaaaag ctgaagaact
    34081 aggaatagag cctcttgcaa ctatagtttc ttatggaaca gctggtgttg accctaaaat
    34141 aatgggatat ggaccagttc cagcaactaa aaaagcttta gaagctgcta atatgactat
    34201 tgaagatata gatttagttg aagctaatga ggcatttgct gcccaatctg tagctgtaat
    34261 aagagactta aatatagata tgaataaagt taatgttaat ggtggagcaa tagctatagg
    34321 acatccaata ggatgctcag gagcaagaat acttactaca cttttatatg aaatgaagag
    34381 aagagatgct aaaactggtc ttgctacact ttgtataggt ggtggaatgg gaactacttt
    34441 aatagttaag agatagtttt agattataat atttacaaat taaatattta attatgaatt
    34501 taacctgtat gaataactat ttgttattct tacaggtttt tatagttaaa aataattatt
    34561 atctattaaa caaaaatagt gtaaaatata taatatcatg attttgaaga tataaaaagc
    34621 aggaagatat aaaagaaagt aggtaaaagt ttgtttaaaa aagagaatca aagatataat
    34681 cttatatcgt atacaaatga agaagaaatg actttaaaag aagttttatt ggacaagcta
    34741 aatttctcag ttaggtcatt atcaaaaatg aagaaggaaa agagtgtttt agtaaatgga
    34801 gtatataaaa aaccaagttt aaaggtgtat agtggtgatt taatagaagt aaaaatagac
    34861 gaggaaaaag caaattttga acctcaagat ttaaatttac agataattta tgatgatttt
    34921 gatataatta tggtgaataa acctccattt atggtagttc acccaacaaa aagccactat
    34981 gataaaacaa tagcaaatgg tattagttac tatatagata atcaaaaaga aaacgtaaaa
    35041 ataagatttg taaataggtt agatatgaat acttcagggt tagttatagt agcaaaaaat
    35101 gcttatgcac atcatacttt atctacagca atgagtgaaa ataaagttga aaaaaagtat
    35161 ataacagtag ttgatggaat tataaaagaa aatgagggaa ctatagatga gccaatttat
    35221 agacctacag aagattcaat aaagaggata atagatgaaa gaggacagtc atctgttact
    35281 cactataaag taatagaaag actagaaaat gcaactgtat tagaagtaag cttagaaact
    35341 ggtagaacac atcaaataag agtacatatg gctcatatag gtcatggaat aataggtgat
    35401 gagttgtatg gttatgtaga tgaagagcta ataaataggc aagcacttca tgcatacaag
    35461 ctagagtttg aacaaccaag aacaaaagaa aaattgaaat ttaaagcaga tataccagag
    35521 gatatgaaag agttaatatc gaaattaaga taatggggtg atatatatga taatacataa
    35581 atttataata catgttttgg acaaaaatag tgatgttcca atattaaatg actttgaagg
    35641 caaagttaat caagaagtag atggtttttt ccagaaagct ataaaaagaa ttgcaaaaga
    35701 tgaggattta agaaaaggtg tttttaaaga ttataatgac aatttaatta aaaactgttg
    35761 tgaacaaata atatatgatg aaagtacttt tttgaaaaat tctaaagaaa ttgcatctta
    35821 cttgtttgac gttatgaaaa ttaatgctgc actagaatct tgtgatttgg caatttgctt
    35881 atacactatt aaagatgaaa agagtgtagc aatactgaaa ttagattata aaaagttgta
    35941 cactcattca attgaatttg tggatgataa atttaatatt caaatggttt caaatgaaat
    36001 tggtatccca gagactttaa gacaaaagca aggagctttg attagtttaa gtggtataaa
    36061 tgatgagttt catctaaggc ttttagataa ggatgctgaa aaagaagggt cagaatctaa
    36121 gtttgtaaca gaatttttaa atgctaaaaa aatagatgat gataagtaca agaccaaggt
    36181 atttaaaaat acagctgaaa attggataac taatgctctt agtaatgata taaagcaagc
    36241 tgaagatgta agaagtatat taaattatac actgaaggaa aaacatgaag ttgatataaa
    36301 tgactttgtt gataattcaa ttaaagatga tgagttaaag gatagcttta aagagcatat
    36361 ggaagaaaaa ggtcttgatg aaagttttag tatagataaa aaatgggttg agaaaaagct
    36421 taaaaaaaga agtataaaaa cggataatgg ttttgacata aaagggaatc taactgattt
    36481 tgaagaccca atgaagtata ctgtaaagca aaatcaagat ggaactatag atataataat
    36541 aaagaatgtt acattttatg aagaaaagta atttatatac ttagtatata aattaaatat
    36601 aagagcgtgt attttatata ttgtaaataa atgtaatcta gtctgtgcga actctaatat
    36661 aatttattat gtagaagttt acacagacta tttatatttg tagacaaaac gatactattt
    36721 gttttcttcg atatatttac atatatcacc aatacattta aagttctcag cttcctcgtc
    36781 tggtatttct attccaaatt catcttcaag ggccattata atttcaacag catctaatga
    36841 atcagcttct aagtcgtcca ttaaagatgc ttccatagtt atctcatcta aattgtctac
    36901 ccctaattgt tctgctatta tttcttttat tttctcaaac attttaagcc tcctaaagca
    36961 gttttaatta attttaatgt tagtttaata taatatcaag aatttttcaa tatggaatct
    37021 ataaaaaata ataaaatttt ataaatgtag taataaagta ttatttgatg aataaacttt
    37081 attgataaca aatataactt aggaggaaat gctatgaaat atgtaggact attactaact
    37141 tcagtaggaa tgtttttact tatagcagta aatttttatt ataattcaat aactttagat
    37201 atgcaacgta tagaggatta tgtaatggag accaatttaa tccttgaaga tgtagctgaa
    37261 aaggagagtt atgtatctaa tgaaaaagaa gactacatat caaggttaat gcatgtaaag
    37321 aaaggaatag aaaattcaaa aacatccttc ttgatagaaa gatataagga gtacaaaata
    37381 aaatcaatag aaagtttaat ctacactata tcagaggaga aaaaagatta tctggatgaa
    37441 gttgatagat acaataaact tggtgaaaaa gaaataaata aactaatcaa taaaaacttt
    37501 ttagaagtaa cttatctgtc tattactaca tatatttaat taaagagaca aaacttagtc
    37561 tcttaatgat tcaaggagga ttagttatga caaaattaga agacaatatg gaacgtttct
    37621 ttggaggatg cgaaggattt tttggtaata aaatgttgat aataatagtt atagtattct
    37681 tactattatg cacagacatt ttagaagact tattatgcga tgacaatata tggatatggg
    37741 ttatattaat agtattgtta ttatttaact tcgatgatgg ttgttgttgc taaaaaatat
    37801 tgattattaa ataggtaaaa ccagtaactt atacaagtta tggttttacc ttttttagtt
    37861 caaattaaaa aatttaaaaa aatagtaaaa atacattagc aaaatggaaa ccaaatgcat
    37921 agtatatgtt aggagcgaaa agctcaacaa aatatcaaca ggaggtaacg tacaatgtta
    37981 gatagattct ttggagaaga aggatgtttt ggtggaggag gatggtggat aatagtacta
    38041 ttcttcctat tcttagcttt tggagatagc tgggcagaat ttgacataat ggcatggata
    38101 ccattcctaa tagttttatt aatactttgc tgctgtggtg gatgcttaga ataagaacac
    38161 agttattaaa aaaaggaggg ctttgttatg ttagatagat tttttggaga agaaggatgc
    38221 tttggtggag gaggatggtg gataatagta ctattcttcc tattcttagc ttttgaagat
    38281 tgctggagag acattgacat aatgtcatgg atacctttct taatattatt gctaataact
    38341 tgttcatgtg gtggattctt tgatggtgaa gtaggttgcg gctgctaata cttaaatcat
    38401 tagaataaaa aaatgctggt attttatacc agtatttttt tattttattt tttaatatga
    38461 tttaaattaa gaatgaatat tattaaattt gttgaatata gggtataata taaaatataa
    38521 tgtatttaaa tgtacttgct tttatggtaa aataaaaact tatataaatg tagttaaaat
    38581 aggaaccttc aaaaatttgg ttcagtttta gataaattta gtaaaataag gaggatgaag
    38641 atgaaaggca atataacgat aaaagatgtt gctaaacaag caggagtgtc aatatctact
    38701 gtatctagag ttataaatga ttcaaaacct gtaactgatg aagtcaaaca aaaagtttta
    38761 gaggttataa aagagactgg atatatacca aatccacttg ctagaagctt agtaacaaag
    38821 aagagtcaat taataggggt aatagttcca gaagtttcag attcttttgt taatgaggtg
    38881 ttaaatggga tagaagaagt tgctaaaatg tatgactatg atattctttt agcgaataca
    38941 tactctgata aggaacaaga acttaagagt ataaatctat tgagagcaaa acaagtggaa
    39001 ggtatagtta tgatttcatg gatagttgaa caagaacata tcaactatat acaaaattgt
    39061 ggaataccag cgacatatat aagtaaaact gctagaaatt atgatatata tacagtaagt
    39121 actagcaacg aagaagctac ttttgatatg acagagcatc ttataaagaa aggtcatgaa
    39181 aagatagctt ttataatgac gagtaaagat gatactgttt tagaaatgga aagacttgct
    39241 ggttatgaga aagcactttc aaataacaat atagaattag acaagagttt gattaagtat
    39301 ggtggaactg attatgagag tggatacaat agtatgaaag aactattaga tgatggaata
    39361 atacctcatg cggcttttgt aacaggtgat gaggctgcca taggtgctat aaatgctata
    39421 tgtgatgctg gatataaggt tccagaagac atatctgttg caggatttaa tgatgttaag
    39481 atagctagaa tgtatagacc taaacttact acagtatatc aacctctata cgatatggga
    39541 gcagtagcaa taagaatggt tataaaatta ataaataagg aattaattga aaataagaaa
    39601 atagaattac cttatagaat tgttgataga gaaagtgtta cagaaagaaa aaaataaaac
    39661 tattaaaatc aatcaggtag tgcctaaggc actacctgat tttttatgtc aataatatca
    39721 aatttaataa tagattaact aaaaataatt atctaattac ttaaatagtt tatttttatc
    39781 tactcatgag ggttagactt tttagttttc ttaaataaac ctttagattt tttttgttga
    39841 ccacctggtg tattttttgg tgcaggtgaa tttgatttac ctttagtagg tttagccttc
    39901 ttagttttag ctggtttagt atctctttga ttttctgcat ctgagatttt ttccattata
    39961 ttatctagtt ttttgcgttg attttcatct aaaaatgctt tgaattttga tactacttca
    40021 tctcttccat ctaagttctt acctattttt ataagttcct ccataagttc attttctgat
    40081 tttcctttgt aactatcagc catcttttct attttatcct tgtcaatatt tttttgttta
    40141 gccatttttt ctaatgactc tgtatctaat tttttcataa aaaaacccct ttctataaat
    40201 atatattgaa ttatattcat actatttata tatagaaata atattgaagt ggtgaattgt
    40261 gagtgatata taatataaat aaaaattctt atagataata tatatgcact gagctttaaa
    40321 aatggaacat taaaatatga aaaacatact ttaatatagg gcaattatat aatataaggg
    40381 ggaattgtct tgaataactt actatttttg attggagtga ttgcattgag taatggtaat
    40441 atctctatat ttgatgataa aaataaatca aaaggaaata atagagttag aaaaaacaaa
    40501 aaaaatgcaa atgataaact ttcacagtct acacatggca atttaaggtt tgatgcaaat
    40561 gatataaaaa gaggtttaag gctcatggat ttaagtgaag aagatttaga aatgggaatg
    40621 gaaataataa caagaactaa aaaatacatg tcaagagatg aaagaaaaat actaataaaa
    40681 atagaaagtg ttttagattt agttagagga ataaaaaaat taaataatat agatatggta
    40741 gatacagaag atgaaacgga ttttttcaga aaaatggata gtgatgataa aaagaatatg
    40801 atgataaagg aaattataga tgtattccca gaaaaaagaa aaaattcagt tgaaaaagct
    40861 ataggaatga aaaagaagat agatttattt gcagaattat ttttaccaga tgactttggt
    40921 gaaggtggat ttagtttgag ctctttggca aatataaaca atcttggtag tatgaataat
    40981 ctcaaattgt taggaagtct tttaagaggt gatgatagta ataatgatga tgaagacaat
    41041 aatgatgagt taaattatga agacgaagaa ttagattgtg aagacaaaga attggatgat
    41101 gatgagttaa cagatgaagg tgaaatggag gataatgaag atgaattagt agaagataag
    41161 aacacaggct atgaaaatat agaaaatgat gaagatatag tatacgatga ggaactatac
    41221 aacattaatg agcaagaatc cgaatacaat tataatgaaa aaaatactaa aactagagct
    41281 aagaaaaaac gataattaac taaatacata aagaaaaaag gaagaaaaag taatgataga
    41341 ataagattat ctcaattact atataaaacc actttaaaaa attcaaaatt tattatataa
    41401 taataatatg aaatcctgaa acatacgata aggctttttt aaattcaacc tacaaatgaa
    41461 atacgggaga ccgtgtctgg taaattataa catgcctagt tagtttggac gttaaaggtt
    41521 actggcaagt cacccacctg ggagtgccta gggtgtgaat ttacttagcc accggtgttt
    41581 cgggacaaaa aaagtgctac tatttcaaat agtagtactt tttttattaa tatgcatttt
    41641 tctactaaat acgcctaatt attgataaat tcaaaaagca agtaacaatt aagattataa
    41701 aagcatagta ttaattaggc ttcaaaaagc caaagactta ataagaatcg cttacaggga
    41761 gggcaatgaa aaatgcaaga ttataaaaaa aataaaagaa gaatgatgaa tcagccaatg
    41821 tctacaatga atgaagaaga agtgtataca gatgaaataa attcagaaga catgagaggg
    41881 tttaaaaaat cacaccatca taatggatgt aatactgata ataagtgtga gtgccatgat
    41941 gagtgtaatc catgtaaccc atgtaatcca tgtaaaccta acccatgcaa tccatgcaaa
    42001 cctaatccat gtgatgacaa atgtgaatgc catgacaagt gtaaatgtga ttgtgaacca
    42061 tgtgaaatgg attcagatga atgttttgaa aacaaatgtg gaccagaatg ttgtaatccg
    42121 atatctccaa gaaacttctc tgtatcaaat gcagtgccat ttgcaataga ggctaataga
    42181 atatttgata ctatgcaatt ccaaacattt acagatgcaa caggtccaaa tggagaacca
    42241 ttaacttttg aaacagaagt agtagaagta tttggttcaa ttccaagtgc aggtcaagca
    42301 agtgtaacta tagaaaaaat atgcttaagt aatgatggaa tagttataga cacaggaatg
    42361 acaactttag aagatttcga tttagaccca ttaggagata tagtaggaag aaactgtgaa
    42421 acaacttttg aatttgcagt ttgtggagaa agaaacgctg agtgctgtag acaaggaaaa
    42481 ggcaaatcag tagcttataa acaaagagga ttaactgtag cagttcgtaa tttagtacta
    42541 gagctaagag gtagatgtgg atgtacagag ttcgttgcat tagctttccc agcagttaga
    42601 gcaggaggtg gatgtaagag aagagttgat tatgtagaat ttacttttaa cacactttca
    42661 gcaccaatat gcttgccagc tgacggaaga gctgttactt taagacaaga atatcaaact
    42721 aacttaactg tagattgtat aggaaaatct atattaaaat tagaatgcaa cgaatgttgt
    42781 gaacctttct atgaattaat tataccaaat gatatagatt tagtactttg cttacaagaa
    42841 acagttagca cattaataag tgaacaaata gtagttttag catcaccaaa tccaatccaa
    42901 ccaagacttg ttgatacttt ctctaaagta tgtgattttt cgcaatgtgg acctaatcat
    42961 ggaagtggaa agccaagttg ccacagatag ataggtaaga tatatagaca gtttaaatta
    43021 taaataaaaa tcatctaagg aaggctacca aagtgtataa tttacattgg tagccttcac
    43081 gtttttataa ctagcgtata gattttagtg atttaaaaag cactatagta tgtaatagac
    43141 aagtaaagat tgatactatc gtcattgtgt atatgtatgc ataaatatta agtcctggaa
    43201 ttggaagaaa tacatataaa gctattagtt gaattaacat gccagtaata gtatttatac
    43261 ttgaagctaa ttcttttctt attgaatgta aaatgccaga tagagtttga tttagggaca
    43321 taaatagtgg aactaaaaac atagctttta gatattctcc tgcaagtgta tttttaaata
    43381 ctaatataca taaatctttt ccaaagaaat aaaaaaatat tgatgatagt atgcctacaa
    43441 ataaagtaag aagtatggag tatttgattt ttttaattac actcttacgc tttcttagag
    43501 ccatttcctg tgaaatagtt ggtattaaat ttacaataag cgctgaacca acagtaaaag
    43561 gtaaaaatat aaagggcatt accatcccac tgacaatacc atacatactt aaagattgtt
    43621 gataagtcat tcctgataat gctaatcttg atggtatcat catagaactt atagattgca
    43681 aaagtatgtt tgacattcta ttacaagtta tgggaataga catttttaat gtttccatag
    43741 aagaattata aaaatctttt atgcttataa tatacctatt gtcaaatgat gaatctcgcc
    43801 ataagcaaat aagcataaat ataatattgc tcaactcacc aatagatata cctaatagag
    43861 ctatataaca attcatagat ttattattta tgtacataac aagtaaaaaa acaaataata
    43921 ttttaccaat ttgttcaatg atttgtccaa tagcagggat ttttacattc tttatgccat
    43981 agtaatatcc tcttagtacg tttgaaagag ttattataac tatagcagga caaacagcta
    44041 gtataaatag atttaattta gcatctttta aaaatttaag tgataaaaaa gaactattga
    44101 aagatgcaac tatagatata attagtgcta cgaaaaatga tatatataaa gttgatataa
    44161 atagagcatt agtattgtgt ctatcgttta aagcttttct ctttgcaact agacaactaa
    44221 gtgcagtagg tatacctgtt gttgtaacag ctaagcaaat catcaaaaag ttaaaaatca
    44281 tatgatagat acctaaacct gtttcaccta gtgctcttga taagaatatt ttatataaaa
    44341 aaccaaatat tcttacaata aaattggata taaataaaat aactgttgat agtactaaat
    44401 aaggtacttt caaaaaaatc acctccttat cttttaaata tattcacaag gaggtgtaaa
    44461 aatttataat tttataaatt ctggtttcaa tttattaaat ttagatacat atttaaatcc
    44521 catatttttt agtaatgaat atatgtagtc aaactcacat gcaacttgac ttatatgatg
    44581 tgaatctgaa cctgtggtta tgatttcccc tcccaagtct ttgtacattt gaagtatata
    44641 cttagatggg gttaggtctg gaagattata tctataacaa gaggtattta tctcgatacc
    44701 tttgccatca taaatagctt gtttaagagt ttcttcaatt atatcagaaa atagtctatc
    44761 atctaaaata gtgtcataag gtgcatacct tttaatcaaa tctaaatgac ctaagacaga
    44821 gtaatcttta taattcttaa caatattata taaataaaga tagtaatttt cataaacttc
    44881 atattgagtt ttatctttaa aataattacc aaggtataag tccattgtat ctatagcatg
    44941 gatagaacat attataaagt ccaatggata ttgatgtgct tcctttttgt atgtatccat
    45001 taattgagtt tgaactccaa attcaattcc tttttttatt gatattttat ctttgtattt
    45061 gctttgaaga gtctctaaac tcttaaaata atcttcgtag acaattgaaa aagaatcatc
    45121 agcatataca tcatagtcaa catggtctgt gaaacaaatt tcctccaaac cgagttcaat
    45181 agattttttt accatttctt ccattgtaga ttttccgtct gtagagaaac ttgagtgcat
    45241 gtgatagtcg taaaacataa atatttcccc tttttataaa aattttggta ttatatatac
    45301 atactaaata atatcaaata tatataaaag catcaataaa atataaatac aatgagcggt
    45361 gataaatttg gaaaaataca gaatagattt aaatgagaaa aaatcaacta aatatataca
    45421 gatatttaat catataaaag aattgataat aaataaagag ttagtagaac atgaaaaatt
    45481 gcctccaata agaaaactat caaatttttt acaagtaaat aacactacta tagttaaggt
    45541 atacgagctt ttagaaaatg aaggatatgt atataaaatt atagggagtg gtacttttgt
    45601 atcaagttca aattcttata aaagcggtat agataacagg aaaaataaga ttattgtgca
    45661 aagagaagat ttaattcatt ttgacaatgg gaatccatct aatgatatgt ttcctatcga
    45721 ttcatttaaa aatgctgtaa atatggcatt atctaaagat ggtagttcaa tatttgaata
    45781 tgatgaaggt cttggctctg aagaattaag agaaaaaatg gtagagtatc taagtgatgg
    45841 aaatataaaa actactaagg aaaatattca aataatatca ggtgctcaac aaggtataga
    45901 tatagtttgt aaaggactta taagttattc agatgttgtt tttatggaag aaccttctta
    45961 caatggagca attgaagtgt ttaaaagtag aggtgcaaaa ataatatcta tacctatgtt
    46021 agatgatgga attgatatag gtattttaaa attaaagttg gaaaaaataa aaccaagact
    46081 tatatacatt atgcctaatt ttcaaaatcc aactggaata tcttactcaa catataaaaa
    46141 aaagaaattg atagaattgg cagaagaaca tgatttttat ataatagaag atgattttat
    46201 aagtgatttt gtttttgaat caaaggacaa tagaacaatt agaagttatg atgataaaaa
    46261 tagagtagta tatataaaaa gcttttcaaa aatacttatg ccaggtctta ggataggaat
    46321 tgtagaaatg ccaaatgagt tgttaaaaaa agttttatgg gctaaatatt catcagatat
    46381 atcaacacca ggtttaattc aaaaatcaat gtattactat atgaataatt ttgattggaa
    46441 gaattatttg agtcatatag agaagatata tactaataag tataaattgt caaaaaactt
    46501 gattaatgac aaattgagtg gtaaattaaa agttagaaaa tcttgtggag gaataaactt
    46561 ttttttagaa ttaccaagag gatatacatc tcaagcattt acaaatttta tgttaaacaa
    46621 aggagtgtca atgcttccag gaacttattt ttttgacaat ctagtggatg atagattctt
    46681 taggataaat atagctcgcc caagcatgga agaactagaa aagggaatat ctgtaataag
    46741 tgataatata gatgagtttt tttgtgaata taaaaacaag ttagatataa aaagcaataa
    46801 attattttat taaataaata gaggtttttt aggaggaaaa aatgtttgac aagaagaaat
    46861 tagataggat taatgaatta gcaaagaaaa ataaagaggg aatcctttct gctgatgaaa
    46921 tcaaagaaag agaaatttta aggaaagaat atttagaaaa ctttagagct cactttagat
    46981 caagactaga tagtgtcaaa gtagtttcac cagaagaata tgagcaatac atgaaaaaca
    47041 ataaaaatta gaacttagta ggaggaaaat atgaacttaa agtacgaatt taagaatgct
    47101 tgggattttg aaagaaaaga aaatactatt gaacaaatta tgcaatattc tcgcaattat
    47161 atggagtttt taagtaagtc aaagactgaa agactttcag taaaagaaat tataaaattg
    47221 gctaaagaaa ataactatat ttcaatagat gaagctatgg aaaaaggaag cataagttgt
    47281 ggtgataaga tttatgttat aaataaagag aaggcagtag cactttttgt aataggtaaa
    47341 aattatatag aaaaaggtat gaagattata ggaagccata tagattcacc aagattagat
    47401 ttaaaaccaa atccactgta tcaagagagt aatctaggtt tttttaagac tcattattat
    47461 ggtggaataa aaaaatacca gtggacagct cttccacttg ctttacatgg tatagttata
    47521 ttaaatgatg gaacaaaagt ggacatatct attggggaag aagatagtga cccagtattt
    47581 tgtgtaactg atttattgat tcatttggct ggagaccaaa tgcaaaagaa actatcagaa
    47641 ggaattagtg gagaagcttt aaatatatta attgggaata ttcctttaga agatgaagaa
    47701 aaagaaccta taacagcaaa tatattaaag attttaaatg agaaatacaa tatagttgaa
    47761 gaagacttat taagtgctga aattgaagta gttccagctg gtaaggctag agatttaggg
    47821 tttgataggt ctatggtgtt gggatatggg catgatgata gggtatgttc atatgctgct
    47881 gtaaaagcta tactagaaac tgaacaacca gaatttactt cagttgcatt atgtgtagat
    47941 aaagaagaaa ttggttctaa agggaataca ggtatgcact caaagttttt tgaaaatact
    48001 gtggctgaac tgattgcttt agaaggagat tattgtgata ttaaagtaag aagagcttta
    48061 gcaaatagta aggtattatc agctgatgtt tctgctggat atgacccaaa ttttggagaa
    48121 gcatatgaaa agagaaattc agcatatatg ggaaatggag tagttttaac taagtataca
    48181 ggttcaagag gaaagagtgg atgtaatgat gctaatgctg aatttatgtc agaagtaaag
    48241 agaattttta ataaaggaaa tgtagtatgg caaactgctg aacttggaaa agtagaccaa
    48301 ggaggaggcg gaactatagc ccatatattg gcaaatcaag gtgcagaggt tatagactgt
    48361 ggtgttggag ttttaaatat gcatgcacca catgagattg tctctaaagt agacatatat
    48421 gaaatgtata agggatataa ggcatttttt aatataaatt tataagtaaa aatataaatg
    48481 taataagtga ataaaggctg ttgtataaac aatttaatct gttgtacaac agtcttattt
    48541 ttatgtaaaa atagcaattt gtcacactag agaaaataaa agaaaatact gtcaaattaa
    48601 aaattattta aaaaaaaatt gacaacgatt tcatcttgtt ataatatgta ataaagaact
    48661 tgttatataa tgttataaaa atgttacata gggaggtgag caaaatggat gaaaagagac
    48721 aaaaaaaaga aatggattta gaagtaaatt cctttgttcc tttatatcaa caactatatg
    48781 acaacataaa aaaacaaata gcatctggca tatataaacc aggagataaa cttccatctg
    48841 aaggagactt atgtaaagaa tttaatataa gtcgtataac tgtgagaaat gcacttaatg
    48901 aacttgtaaa agaagatata ttatgtaaaa aacgtggtaa agggacttat gtaacaatac
    48961 cagagagaat agaagcaaca tgtgcaggaa atagttttac taattcttgt catcgtatta
    49021 atgcaaaacc aagtaccaaa ataatctctg ttttaataaa aaaagcagat aagcaagtag
    49081 ctgaagcttt aagtattgaa ctagaagaga aggtaatatg tattaaaaga cttagattaa
    49141 tagatgaagt acctgttatt tttgaagttg actattttag aatagattac atgtttttat
    49201 taaaagaaga attagaagat aaatctttaa tggaggttat atctaataat ataagcacat
    49261 tacctaaacg agtagaaaat atatttgaag taaaacattc aaacaaagaa tattctgact
    49321 atcttaaatg tgcaagtaat atgccactat tgaaagtaag acaatcggtg tatacagaaa
    49381 ataatgatgt cttatattac aatgagcaat ttataagaag tgataagtac aaatatgcag
    49441 tatcagcaga aatataatct atatttagta ggaggatgaa gtatgaaaaa gataatatta
    49501 gcatctcatg gagatttttc aaaagggctt ttaaatgcag taaaaatgat agtaggagat
    49561 ttagcagatt gtgttagttc gtatggtttg taccctggac aaagtgcttc agattttgct
    49621 ttagaattag aaaaaacaat atcagaagat atagaatgtg aatatataat tttatctgac
    49681 ttatatggag caagtgtatg tacagcaatg ttaagattaa ctaatctaca taatgttaag
    49741 ttattttctg gaatgaattt gaatatggta ttagaacttc ttacaaggtt ttctgatgaa
    49801 ttaacagttg aagatataga acaattagtg gaagaatcta gaagaggaat acaaagcgtg
    49861 acattacaaa ttaaagaaga agaagaggta ttttagttta tgaaaaaaaa gggaatatta
    49921 tcagtattta aaagtaaaaa accaattata gcaatgatac acttaaaagg agatactcca
    49981 gaagatatct ttgaaagagc taaaaaagag attacaatct ttgaagaaaa tggtgtagat
    50041 ggaataatgt tagaaaatta ctatggtaat tattatgatt tagaaagaat acttgaatat
    50101 gtatcaaaag ctaatttgtc aataccatat ggagtaaatt gtttaaatgt tgatactatg
    50161 ggatttgaac tggcaacaaa atataatgct agttatatac aggttgattc tgtagtaggt
    50221 catgttaaac ctagagacga agctacttta gaagaatttt ttaaattaca acgttcaaaa
    50281 tgcccagcgt atttaattgg aggcgtacgt tttaaatatc agccagtatt atcagaaaat
    50341 gacgttgaag aagatttaaa aataggaatg acaagatgtg atgctatagc agttacagaa
    50401 aatgctacag gtcaagaaac atcaatggaa aaaatagaac tgtttcgtaa aaatttagga
    50461 gactttccat tggttatagc tgctggagtt acattagaaa atgcaaaaaa acagcttgaa
    50521 ttaggagata tggcaataat aggaagttac tttaaagata attacaaaga ttttgggaat
    50581 gttagtgtag aacacgtaaa aacattcatg gatgaaatca aaaaaattag ggaggaatta
    50641 taatgattaa attagtaaga gtagaccaca gacttataca tggacaagta gcatttacat
    50701 ggacaaagtt tttaagcaca gactgtatat tgatagcaag tgatgaatta ttaaaagatg
    50761 aattaagaat ggcagggctt aaaatggcta aaccatctaa tgttaagtta gtaatgaaaa
    50821 gcatagcaga ttctataaaa gcacttaact caggtgttac tgataaatat aacttattga
    50881 tactttgtga atctgtagaa gatgtttata gacttgctaa agaggtaaaa gctattaaat
    50941 ctataaacct tggtggaaca aaatcagatg ataatcgtga aaatatatct aaagcagtgc
    51001 atgtatcaaa agatgatata aagatgatta aagagttaga ttcagaaggc gtaaatgtat
    51061 ttgtacaatt agttcctgat gatgatgcta caaatgtaat gaaattaata taattttagg
    51121 gggatgaaaa tggaatttac acaagttatt ttgataactc taattgcatt ctttgcatat
    51181 atgcatagtt ttgtaggttc tacaatgcat aatagaccta tagttgttgc accacttgtt
    51241 ggtttagcac taggtaatct tcatacaggt atagtaatag gttcaacatt agaattagta
    51301 tttatgggtg catttcctgt tggggcaagt aatcctcctg attttgtgtc tggttctatt
    51361 attgccacag cgtttgtaat attaacgggt caagatgtat cagcagcagt tgtacttgca
    51421 gttcctatag caactctagt attgcttata gataacttct taatgacagt agtacttaca
    51481 tggggagctc atattgctga ccgttatgca gaagagggga atattgaagg cgtagaacgt
    51541 gtgcaattat tatttggtat aggaaataaa ttaatattag caataatagt aggtatagga
    51601 ttttcattag gtgttccagt aatagaaaaa atactttcat ttataccatc atatgtaact
    51661 catggaatgg atgttgcagc aggcgtgatt ccagcaatag gttttgccat gttagctagg
    51721 atgatgctta ataagaaaac agtagcattt ttattattag gatttatatt ggttgcttac
    51781 ctaaacataa cagtaacagg agttgcattg tttggattag caatagcttt aatatatgtt
    51841 aactttgtag gagagaagga ggtaatagta gatgacaacg agttctaaga agttagatac
    51901 aatttcaccc gatagtaaaa taacgaggaa agacttttgg aaatgcttta gaaggtcatt
    51961 gacattagat tcatcttgga actacgaacg tatgcaaaat atagcatatg catacatgat
    52021 ggcaccaata atccgtagat tgtataaaga tgataaggaa aagaaatcta aggctttaaa
    52081 aagacattta gaatttatgt cagttactcc tcatataagt actcttttgg taggtatatc
    52141 tggagctatg gaagaagaaa atgcaaagaa taaagaattt gatgctaata gtataaatgc
    52201 tgttaaatca agtttaatgg gtccagtttc tgggatagga gattcatttt tctggggaac
    52261 attaaagctt atagcagctg gagttggtat agctttagct tctcagggta acataatggg
    52321 accgatttta ttccttttaa taataaatgt tcctcatttt attatacgct atatatgtct
    52381 agataaagga tttaagtatg gaactcaatt ctttaaagat gtgagtagtt caagtatagt
    52441 ttcaaaagtt atggaagcag cttcaatgtt agggcttatg gtaattggag gtatgactgc
    52501 atctaatgta atgttaaaac ttagtgttaa tgtaggtagt ggagaatggg cagagcctat
    52561 tcaaacttac ttagaccaaa taatgccatg tatgcttcca gctatgatat tcggtattat
    52621 gtattggtta ttaggaaaaa aggtaaagac aactacgata ttaatttcag ttatgattat
    52681 ttgtatagta ttagctgcta ttggaatagt ataaataaaa aaatcccaag ttcatttgaa
    52741 cgagggattt tttgactaaa ttaaataaaa aagaaagatg gttcataata atttataagc
    52801 atttatgtga tagttataat tagcttatta attagttata aacccaaaat taagcactac
    52861 tttattaaag ttattatatt ataatttcga aatactcaca taatctgttt agaaatttat
    52921 cttctaatgg ggaaaaagtt ttctttttag agtaaataaa gaaaaatttt ctagtaaaat
    52981 caacaccctt tattttataa cattttattt tacctgcatt gagataatca atagctgaaa
    53041 tgtacgatat aaaggagact ccaagaccta atctaaccat ttccttaata gcctcatttg
    53101 attctacatg agctattaca tttaatttac ttactggaaa gttattttta ctaagtgtgt
    53161 ttagtattaa gtttcttgta cctgaccctt cttttctcat aataaaattt agataagcca
    53221 attctcctat atcgatacaa ttatttttat tatcaatttt aaaatctgaa ggagtgataa
    53281 gaacaagttc atcatctaat aagtctaggt actctatttg aggattattg attttagaac
    53341 caactaatcc aaagcttatt cgttcattta aaatttctga tatagcatat tgagaatcat
    53401 aatggcttat ggaaaatttt acatctggat aactcataga aaaactcttc ataaaatcag
    53461 gtaaaatata agtttcaggt atggaactac atgctatatc tataatacct tctatttttc
    53521 ctgaatattc ttttatatca taaattgcac gtttgcaatt attcaatata tatatagcat
    53581 gttcataaag aatttcacca gattttgtga gtgtaataac tttgttgctt ctatttataa
    53641 gaacagtttc caactctctc tctaaattct gtatgtgtgc acttacagta ggttgagtta
    53701 aaaaaagttc tcttgcggcc tttgaaaaac tctgatgttt tgcaacagca acaaatactt
    53761 caagttgttt aaaatccatt atttaatcct ccttaaatta tctaaaacta ttataacata
    53821 tttgataaat taatagtcaa aggtgatttt taaaatagaa aacctttaaa tattcatgga
    53881 gtttttgatt gtttaggaat aatttggtat aatattgtaa ggtacttaca ataaaaatat
    53941 cgtaaattga attttcaaaa ttcaacttta aagtgttttt tattacagaa aaattacaat
    54001 tagctt
//
LOCUS       DAEPOG010000007        50785 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEPOG010000007 DAEPOG010000000
VERSION     DAEPOG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.007"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(175..954)
                     /gene="hisJ"
                     /locus_tag="KR432_000362"
     CDS             complement(175..954)
                     /gene="hisJ"
                     /locus_tag="KR432_000362"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBH0638918.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1494..1844)
                     /locus_tag="KR432_000363"
     CDS             complement(1494..1844)
                     /locus_tag="KR432_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBH0638919.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(1860..2276)
                     /locus_tag="KR432_000364"
     CDS             complement(1860..2276)
                     /locus_tag="KR432_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBH0638920.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(2295..2486)
                     /locus_tag="KR432_000365"
     CDS             complement(2295..2486)
                     /locus_tag="KR432_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBH0638921.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(2637..3452)
                     /locus_tag="KR432_000366"
     CDS             complement(2637..3452)
                     /locus_tag="KR432_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH0638922.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(3499..4437)
                     /locus_tag="KR432_000367"
     CDS             complement(3499..4437)
                     /locus_tag="KR432_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0638923.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(4434..5306)
                     /locus_tag="KR432_000368"
     CDS             complement(4434..5306)
                     /locus_tag="KR432_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0638924.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(5331..7259)
                     /locus_tag="KR432_000369"
     CDS             complement(5331..7259)
                     /locus_tag="KR432_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBH0638925.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(7371..7748)
                     /gene="gcvH"
                     /locus_tag="KR432_000370"
     CDS             complement(7371..7748)
                     /gene="gcvH"
                     /locus_tag="KR432_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH0638926.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(7835..9961)
                     /gene="cdhC"
                     /locus_tag="KR432_000371"
     CDS             complement(7835..9961)
                     /gene="cdhC"
                     /locus_tag="KR432_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBH0638927.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10018..10824)
                     /locus_tag="KR432_000372"
     CDS             complement(10018..10824)
                     /locus_tag="KR432_000372"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0638928.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(10874..12241)
                     /locus_tag="KR432_000373"
     CDS             complement(10874..12241)
                     /locus_tag="KR432_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBH0638929.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(12272..13216)
                     /locus_tag="KR432_000374"
     CDS             complement(12272..13216)
                     /locus_tag="KR432_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBH0638930.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(13236..14006)
                     /locus_tag="KR432_000375"
     CDS             complement(13236..14006)
                     /locus_tag="KR432_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0638931.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14062..15447)
                     /gene="lpdA"
                     /locus_tag="KR432_000376"
     CDS             complement(14062..15447)
                     /gene="lpdA"
                     /locus_tag="KR432_000376"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0638932.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(15491..16372)
                     /locus_tag="KR432_000377"
     CDS             complement(15491..16372)
                     /locus_tag="KR432_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH0638933.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(16408..17082)
                     /locus_tag="KR432_000378"
     CDS             complement(16408..17082)
                     /locus_tag="KR432_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBH0638934.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17119..17991)
                     /locus_tag="KR432_000379"
     CDS             complement(17119..17991)
                     /locus_tag="KR432_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBH0638935.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18083..18715)
                     /locus_tag="KR432_000380"
     CDS             complement(18083..18715)
                     /locus_tag="KR432_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBH0638936.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(18820..20496)
                     /locus_tag="KR432_000381"
     CDS             complement(18820..20496)
                     /locus_tag="KR432_000381"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBH0638937.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(20745..21524)
                     /locus_tag="KR432_000382"
     CDS             complement(20745..21524)
                     /locus_tag="KR432_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0638938.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(21834..23753)
                     /gene="cooS"
                     /locus_tag="KR432_000383"
     CDS             complement(21834..23753)
                     /gene="cooS"
                     /locus_tag="KR432_000383"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0638939.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(24360..25271)
                     /locus_tag="KR432_000384"
     CDS             complement(24360..25271)
                     /locus_tag="KR432_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0638940.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(25387..25509)
                     /locus_tag="KR432_000385"
                     /pseudo
     CDS             complement(25387..25509)
                     /locus_tag="KR432_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(25592..26734)
                     /locus_tag="KR432_000386"
     CDS             complement(25592..26734)
                     /locus_tag="KR432_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0638941.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(26752..28506)
                     /locus_tag="KR432_000387"
     CDS             complement(26752..28506)
                     /locus_tag="KR432_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBH0638942.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            28642..28848
                     /locus_tag="KR432_000388"
     CDS             28642..28848
                     /locus_tag="KR432_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638943.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(28853..29776)
                     /locus_tag="KR432_000389"
     CDS             complement(28853..29776)
                     /locus_tag="KR432_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638944.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(30030..31730)
                     /locus_tag="KR432_000390"
     CDS             complement(30030..31730)
                     /locus_tag="KR432_000390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH0638945.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(32141..32554)
                     /locus_tag="KR432_000391"
     CDS             complement(32141..32554)
                     /locus_tag="KR432_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0638946.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(32622..35000)
                     /locus_tag="KR432_000392"
     CDS             complement(32622..35000)
                     /locus_tag="KR432_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0638947.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            35317..36768
                     /locus_tag="KR432_000393"
     CDS             35317..36768
                     /locus_tag="KR432_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBH0638948.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(36972..39485)
                     /locus_tag="KR432_000394"
     CDS             complement(36972..39485)
                     /locus_tag="KR432_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0638949.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(39708..40529)
                     /locus_tag="KR432_000395"
     CDS             complement(39708..40529)
                     /locus_tag="KR432_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0638950.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(41048..41524)
                     /locus_tag="KR432_000396"
     CDS             complement(41048..41524)
                     /locus_tag="KR432_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBH0638951.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(41558..42136)
                     /locus_tag="KR432_000397"
     CDS             complement(41558..42136)
                     /locus_tag="KR432_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0638952.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(42240..44858)
                     /locus_tag="KR432_000398"
     CDS             complement(42240..44858)
                     /locus_tag="KR432_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0638953.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(45350..46033)
                     /locus_tag="KR432_000399"
     CDS             complement(45350..46033)
                     /locus_tag="KR432_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBH0638954.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(46375..46965)
                     /gene="zapA"
                     /locus_tag="KR432_000400"
     CDS             complement(46375..46965)
                     /gene="zapA"
                     /locus_tag="KR432_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH0638955.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(46990..49383)
                     /locus_tag="KR432_000401"
     CDS             complement(46990..49383)
                     /locus_tag="KR432_000401"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH0638956.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(49400..50419)
                     /gene="pheS"
                     /locus_tag="KR432_000402"
     CDS             complement(49400..50419)
                     /gene="pheS"
                     /locus_tag="KR432_000402"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH0638957.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(50469..50703)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        15308 a         9900 c         5589 g        19988 t
ORIGIN      
        1 tattattcta ttgaatttta ttattatttt acttagattc tattgttatt ttacttagaa
       61 cttattgtta ttttatttaa gttctattac tattttcaat catataaatt tacaaaagac
      121 tgtacataaa tttattttat acaaacttat ctacagtctt ttgtgtgaat atatctaaat
      181 aatatcgtat ttatttattt cctctgcaca gtgagaatca gaacctaaaa ctaatggaat
      241 atcgtatttt tttaccaact ctaataagta tccatctata taaggctctt tgcaatactc
      301 ttttctattt ccagaaacat tataatcaag ttcataacct ttttctttaa ttaactttat
      361 taagtcttca agaattgtat ttcctttata atcgtatgga aatacctgat taaactttct
      421 aactaaattt aaatgaccta ttctttttgg tttatgtata cctaaatctg aatttacagc
      481 ttttatcaat gtttcataat acttattata aacagcttca atacttccta atttgtcgat
      541 taaattttga aactcctcta cactgtaatc tacacaataa taatcatcat caattttaat
      601 tatatgtact gataaaagag agtcttctaa ttcctttcca tatttattta aattctcctt
      661 tatcccttct tcgtatcctt ctataaaatc cacttccgac ccaacattta tcttgatttt
      721 accctcatac ttttctttca acttcttgac atcattaaaa tactttggta aatcttcaat
      781 actcatacaa ctatcatttt caggagcagg gtctctaaat ccatttgcat acggaaaatg
      841 ttctgtaaat gtcatttctg ttattccaac atccattgct ctttgtacat atttttcaaa
      901 atcatcctta cttccatgag gacaataagg tgaatgtata tgaccatctt tcataaataa
      961 ttcactcctt ttttaaagaa aaaatctaat ataaatgcat aaatttttta acttcaaaat
     1021 aatattttta atcatgctaa tttttattat atctaatatt agagtaattt taaactgcct
     1081 tttaagtatc ttatctttag tactattttc aacttaacat taatattatt ttttaactta
     1141 acattaatac tatctttaaa tagtaatact caatatataa ttaatcaaat aactcaaatt
     1201 gtttgatgaa gcttttgcag tatttaaaga atatataatt atttaacatt aaataatcca
     1261 atcttgtaat aaaatttact cattaaagta tattacagaa aatatctcaa aataaacttt
     1321 ttagttcatg atatactctt ttttgcatga aattagatat atttttatca tttcaacaat
     1381 gaaagggagg cttacatcca tcttgagata accccttaga taaaattttt aataattaaa
     1441 catcataaaa tataaaaatc gcttatatca aacgtataag cgattaatta tatttacata
     1501 tgttttttag cctctaacaa cattttatta gcttcttcca tatactctat agccttttca
     1561 atactctttg ctgtttcttc ttttccaata gctctagcct tttcaaccca ctctctaaat
     1621 ccttcttcat gagtttcatt gtgatttatc caatgtacta aaagtatttt taaagttttt
     1681 tcatccttag actctgatga ttcctcatga ctatgttcat ggtcgtggtc gtgagcatgg
     1741 tcatgtggat gttcatgaca tatatctcca tgagtatgtt cgtggcagtg gtcatggtca
     1801 tgatgtccat gatgatatct tacaatctct ttatcaaaaa tcattagtta ttccccctgt
     1861 tattttatat taaactcttt ttttataagg tttacagaat cttttacaag tagtgctaat
     1921 ggtttacctt ctaatccaac taactccaca ttttctggca taataggtaa aagtatctta
     1981 agtgcctcac tgtctgcaat agcttcactt atattacaag taacttctcc catcatagaa
     2041 ttaggtatta ttacagaaat aggtgcaact attatatttg cttttttagc tgatactact
     2101 atagcatttt cccctgtagc tcctttatta gcatgagctt tcatcataga actagttgct
     2161 atagaattag tccctagtgc ataaatttct acatatgtag gtaactcttc tctcaaagaa
     2221 gaaactatct gggctccaat acctccaccc attccatcta tcactgctat tatcatatga
     2281 aattccccta ttaattaatt ttcttgtatt attatcttat ggtccatcaa tcttatttct
     2341 ttaattatcc catctattat cttttgctct cctaataaat ccgttaaata aatctttcca
     2401 tcacaagggt ctatattaac cacattttcc ataacctttt ctaactcatt attactttta
     2461 caaatgaaag ctgatgattc acacatataa atctcctcct aatatgattt ttattaaaaa
     2521 tcaaaaaaag attcccttag gtatttcaat actccaaaga aaccttcatg atttcagttt
     2581 ttgtatatta ttatacctta agtatatcac tttaatttat gtaaagtcaa ttaaagttaa
     2641 cgctttttat tatactcttc attcttgtat ttttcatata gttctttaac taattgcttc
     2701 tcataatcag tcaaatcgcc ctccacaaac tcaacattaa attgttcagc taatcccttt
     2761 actatgtttt tctgtagaat atctatgtct atttttctgc caatttcatt ttctattcca
     2821 cttgcttttt tagctgtaaa tttcatagct ctttctttca attctggtgt cttagttttt
     2881 attaatttaa acaatttttc tacatcaaaa tccagtatta ttgaaccatg ttgtaataaa
     2941 acaccatctt ttctgctttg agcacttcca attaccttct tattattaat agtaacttca
     3001 tatgatgcat gtgcattaaa acatgcagct gataaatttt ccctacttat tctctcgcct
     3061 ctatttaaat tatctgtttc tataccactt agattcaatc ctttaactag accttcactt
     3121 atatatctat aagataggtt gatgctctta tcgattaaag gattatcttc tcctataatt
     3181 atactataag ttagctcatt atcatgtaat acagctcttc cacctgtaat tcttctagta
     3241 tagtctatat tcatacatct acacttatct aaatctatct catcttccaa tttctgaaaa
     3301 tatcctatac tcaaacaagc tggttcccat gtataaaatc taagagttgg cttattatgt
     3361 cctttcttat atgcagtaaa tatagcttcg tcaattgcca tattcattgc tccttcatat
     3421 gatttattat gtataactct ccactgattc atgattatta cgcccccatt tttagtataa
     3481 atatatccta gaaatcctct atagatttat ttctcttatt gctaaatcta tctcactttg
     3541 atttaaaggt cttgaatagt tgtatatagt acttccgggt ctttcattat agtatggtct
     3601 attacaatcc ttacaacctc ttatttcaaa aggatagcct ttgtttatgt cttttaaaat
     3661 atcattgtca agctttatac tatttaagta tccattttta aacttaaaat actcttttgg
     3721 ataaccttta tttatcatat aagacattaa ctgtaccctt ctataacttt ctatgcttgg
     3781 ttgatttatc ttttccattt tagttcctct aactggagta aatgcaaata aactaatcgt
     3841 tacatcattt tcctttaaat aaatatataa attgtatata tcttcatgac tttcacccat
     3901 accaactatt atatgtgtac ttattttatt gggatatgat tttgacattt cagttataaa
     3961 ttttaacttt tcatcatagt tatttccttt tattttttca tatagttctt tatttgatgc
     4021 atctattgct attcctattt tatcaactcc tactgaaaaa aacttcttta tctgttcatc
     4081 attttctaat tttgcagata gtgaaattgg catgtctatc ttaccactta tatagtttat
     4141 aaagtctaaa acagagttat gtgcttcttc acttgccatt gattgaatac atattctctt
     4201 tatatttttt cctttataag cttttaaagc atctaatatt tcttcttttg aaaactctgg
     4261 ccaaattact cttgataatt tatcttttct agtactactt tctattgatt gagaacaaaa
     4321 actgcattta tttatacatt tttctccaat cattatatat gctgtggtag gaggtatatc
     4381 gctttttttg tttaatatcc caagctctat tgctgtccca actgatagtc taatcataaa
     4441 acgcaacact ccttacactc atttattgtc atattcattt ctatagcctt attctttgca
     4501 gaccttgatg gtaatactat tctattgact ccacacatta tagataattg gtctagctct
     4561 tttctataca ctcctcttgg tctcatacaa cctagattaa tttcagtatc tggtaaatta
     4621 attctagctt cacataaaat atctgctacc ccttctaact caggaggttc tacattttca
     4681 tactcagtcc cttttgttgg aataaggact ataaatgtaa gcttattagg aggatttttc
     4741 tgtaggtatt ctataatttc atattcaccc tttatttgtc ctccttttaa accaatacat
     4801 atatgtggtg ctacttctgt atgttcttgt attgtattat aaacttcaat ataatcttct
     4861 ttactctttt tcatcttata aacttctctt attgtctcat catcaaacac taaatcaaaa
     4921 gaaactatat ctaaatactt acacaactct acaatgctat ctttatccat aagacctaaa
     4981 tgagcattca atctatatcc tttctctttt aagtttttaa tggtattaat atgagtattt
     5041 atagggacat ctccctcata actacaaccg ccactcaata gaaagctagt tatattcctt
     5101 gattgtactt tttcctcata ttcttctatt ggcaccatat tgtttaagta gtgaccgttg
     5161 caatgggcac atttcaaact acattgattg cttgtagtac taagagctag tgtctggtta
     5221 ggataagcaa actctatttt atttccaaaa ttctttaatt taatatcaaa tgctattttc
     5281 attttttcag atagattctt attcatagtt tattccttct taagtacatt ttactcttct
     5341 agtaatttct ttgctgttgg gaactgctct atattcgtat gtggtaagaa acatgcagaa
     5401 ataaattcat ccatataact tggatataca ctaagttcaa cataagttat ttgattacct
     5461 atttcttcaa gtttattttt tgcatcttta cttatgagag ctaagtaaga accaacaagt
     5521 gaactattcc ctatataagt aaatttttct ctatcaatgt caggaagtaa ccctataatt
     5581 attgagtttt ctatgtctag gttgtttcct ataccacctg ctatataaac cctatctaat
     5641 atactaaaat ccatacctaa actatctaca agagtgtatg cacctgaata tatagctcct
     5701 ttagctctga tgaaattatc tatatcaact tcattgacta ctatatcatt ttctaaatca
     5761 aattcttctt taaatgctaa tacatactct cctatctcat gctcattaaa tctaactctc
     5821 ttattatcta aatccctgta tattttacct cttctatcta ttacaccttt agtaatcatt
     5881 tgacagatta aatcaattat accagaacca catataccaa ctggtgcaca ttcatcaata
     5941 atctttaatg ttggctctaa agtttcttta tcaataatta ctttctctat tgctcctgct
     6001 gatgccctca ttccacagct tatccctcca ccttcaaaag caggtcctgc tgaacatgca
     6061 cagctcatca tataatcttt atttccaaat acaatctctc cattagttcc taaatctata
     6121 aataaaacat tttcttcact tgaccatatc cctgctgata atacacctgc tgttatatct
     6181 cctcctacat aacttgcaac agatggagct aagtacacat atgcactgtc atttacaaaa
     6241 agaccaacat tttcacccat aagttttgga gattttaaaa atggtggtat gtaaggttct
     6301 tgtcttaaga agtctgtata cacacccaag aataaacttg tcatagttgt attcccggca
     6361 gccactaatg taactacatc ttccttatct attccatttc tttcatatat tgtttttaat
     6421 agtggattta tagtttcctc aactatagct ttatgaagtg tctctaatcc atttttctta
     6481 gttgaatata taattctatt tataacatct gctccatatt ttatctgtgc atttccagaa
     6541 gatgctttat ctactacctc ttttgaatat aggtctacta aacaaacaac tactgaagtt
     6601 gtaccaatat ctattgcaac tccgtataag gaattttctt tattaccttg ttctatattt
     6661 atgattgtaa gtttcttttg tttttgaaca tatgttattg taaccttaaa atcactcttt
     6721 ctaaatacag ttggcatttt tctaagtata tccaatctaa aatctatttc attatatcct
     6781 aggctatttc taacatatct ttccaatcta tctacatcac ttatattatc atctaaattt
     6841 ggttcttcca tttctatgta tttctttttt atatttgttt taaattgaag gttatgttct
     6901 tcaattattt ttttagctct ttcaaaaatt tccctatcct cttttttatt actaccttct
     6961 atcttcatac catgcataga agatgatact ttagaaggaa cttcaatttc tatatctgat
     7021 attacctttg tacaacaagc tagtatataa ccttgctccc actcatcatc tgttatatgt
     7081 cttgtttttt ctgtatcaac ttttccattc aataatttta ctttacactt cccacaagac
     7141 acgtttccat tacaaggagc atctatgaat atatctgcat ttcttgcaac ctctagtaat
     7201 atatcaccct cattacaata aacctcttta ttgtttggtg taaaactcac ttttatcatg
     7261 ttatccctcc caatttgttc tatatatgat aaaaggaaag tatagctaaa tttgcgtgca
     7321 ctcaaatttt actatacttt ccccaattat aataatatct taatctttaa ttattctaat
     7381 ccagcctcgt attctttatc acttaataac gtatcaagtt cagccacatc agctagtttt
     7441 acttttaata tccagttttc atatgggtct tcatttatac attctggctc atcttctaat
     7501 ttttcattaa cttccaatac ctcaccatta acaggagata ttaaatcaga agctacttta
     7561 gaagactcaa ctactccaaa atctactccc ttagttacag tatcttctac ttctggtgtt
     7621 tcaacaaaca atatttctcc taattgatct tgagcatagt ctgttatacc tatatataca
     7681 acatctccat caattacttt tacccactca tgatctttag agtattttaa ttctggtaat
     7741 aatttcattt cgatagaccc ccaatattat attattgatt aaattactta aatcatatta
     7801 atattatatc atattatttt aaattttcca tcaattacat tacactttcc atagctaatg
     7861 ctggatgacc tttttcttct aagaaagcta atactccttc agaatcgtca gctatagttt
     7921 catcacaaac catatcacag aagttttcta ttccagtcat ttctttaact gtagcattta
     7981 atttatcagc tacataatct tttaattctt ttggcatcca aactattctt tctggtccac
     8041 cttctgcata agcgaatttt ttagaagata tgaaatgtct tccgtgtccc ataaatcctg
     8101 gagtttgaac tcctccacct gtcatagaag caagttctcc gaaagtcata ccaactggag
     8161 taacacttgc aaattctctg ttaactacaa caaatccgtt tgcttctggc attataccac
     8221 agatacattc gaagcatcca caagaagtca ttgggtcttc tagtatagaa tataatgtaa
     8281 cagattcaac tgcaccttga gatatttggt ttacagtttc atttactgaa ttccatacac
     8341 ctgttctatc atctaagcac tcgccttttt ctattggttg acaaggacct gttgggtcta
     8401 attctttagt agccttagca tctaaccagc taactgctcc acaaagacca agtctttcag
     8461 gagtaacaac acaaacgtga gctggtgcaa atgattgaca taagttacaa gaatagaaag
     8521 tatcaacact ttcatcaact aatgaagcta atctttcatc tctagcattg tacttagcta
     8581 ttgcttctcc ttttaattca gacacttttt cagcatctgt aattatagtt atttcacact
     8641 tatcaactac tgattcaaat tcatctaaca tcttagcata taaaacttct cctatatgct
     8701 caagtctaaa tcctttatca aatgcatctt tagagattct tacccaagcc atatctcttt
     8761 gtccaacgtg cattacacct tctatatagt tcaagaagta gtggaaacgt ctttcaagaa
     8821 ctggctcgaa atcttcttgc atatttttac cagctatttt aactattaca gcaagcggta
     8881 atcttaatac accatctgca tcaacttcat ctatatttgg tcctattatt tctatcttat
     8941 ggtcttcaac ttctgttgct tctttcttaa caacaagctc ccaagcttca gttctgtttc
     9001 caccaaactc agcaaacata tcgctctttc taattctctc accttcaaat gctgctgcaa
     9061 atgaaactgg gataggtatt tcagtaactt ttatttttat tcctctagct tctaatgaag
     9121 tagctactat cttatcgtaa tctttttgag ttaataggtt cattggaact tcaagaactg
     9181 tttggtcagt tataactggg aatcctaaag ctattgctcc agcacctgca gatacaacta
     9241 attcacttaa tggtccaaat gcattaacaa atgcaggaac tctattagct gtgtactcta
     9301 ataatccgtt taaatcacca ggagttaatc caccgaatat taatgctgct cttactgcaa
     9361 cagatacaac gtgtataaca gaagttacat catatcctag aggtataact cttagctcaa
     9421 gacccatttt tactccagct tctatagctt ggtctataac tttaccaact aagaaagtta
     9481 gtaaaccttt agattggtaa tcttttataa cttttgctgc tgattctgca tctggacact
     9541 caccaagaac aactgcaaca ccaggtatat ctcctgtaac taatggtaca ccaagtgatc
     9601 tgattatagg gtcagttata tgacctgcac atggctcact atatggagca tccatagttg
     9661 aatattttaa tgcttcaatt acttctgcag ctaaagccgt agctaaacca gcattaaaag
     9721 catgttctag taaatgagtt ctatttatta aagattttac tacttctaaa gcaccttcta
     9781 agtcccctaa agtgttcatc ttttgtcctg ttgcagcata tatacaaggt aatgaatatg
     9841 ctgtatcagg gaaagcaact ttatgctctt ttccattctt ttctatagct tcagctaaca
     9901 tagcttgagc cgcacctaaa gcttgttctg accctgtaaa gattatatta tatagattca
     9961 ttctatttcc ccctttaata tttacaccta ttttttatta actataccct aaaaacccta
    10021 gaaagcaaat ccaccttcat ttaactctaa gaaagagtct gcatataaag atgccccgtt
    10081 gattatgtca caagatttta cagttttcat taattctggg tcacatggat tcattatagc
    10141 tgaactaaat ccattagcca ttgccattgc taagaatgca ctatctaaaa caggtctaac
    10201 atgcttagga catccattag atacgttaga taatcctcct gtagttaaaa gtcccatctc
    10261 agtcatcatt tttatagctt ctaaaacttc tacttgtttt tcttgcatac cttttataac
    10321 taagcataat gggtcaaata gtatatcagt tggctccatt cctaacatta agcctttttc
    10381 taatatttct tggcagtaag ccatacgctc atcattatct cttgggatac cttcttttgc
    10441 acataaacct ataaccattg cttcgtattc agctgctata tctattaaat caaatcttga
    10501 gccagcatca gcagagttta ttattggttt tgcattagtt ctatcataaa ctttaagtcc
    10561 agcttcgata gcttttttat tagctgtatc taatgctata ggaacattgt tgaactcaga
    10621 ttgaagtaat ttgattcccc atgtcattat ttcttcgcca tctctttcgg caggtcctat
    10681 attaaaatct atatagtgtg ctcctgcttc taattgctct tttgctcttt ttaagattgg
    10741 agctgggtct ctttctgcta atgcttttct tattgaaggt gatatacaat gtattctctc
    10801 acctataatc ataaattttt ccatttataa tgtccccctt taaatttaat attaatagtt
    10861 agttaatatt ttattagttt gtttggcatg ctttttcttg gtatcctttt aagaacttag
    10921 gtaattccat agattcctct ggtcctataa ctacattcca tcctggtaag ttatcttcta
    10981 tatcaccttt aagtactgca acttttcctg gaatgataag gtctttacaa ttagttactt
    11041 cttctacctt actttcttta atgaaagcag agattgagtt tccaccaaat ttaccagcag
    11101 cccaagatgt aagaactgaa tatccacctg catctggtat tactaaccat actggaactt
    11161 ttgatctttc tatatcacca gcaactatga agtaagttaa tgcaaagtca acagtaacta
    11221 atactggaga attttcatct gggttgttga ttggataaat ctttggttca actctcattg
    11281 gtctttgtgg gtcagtgtat atgttttgtc ttagtgcaaa taatggtaat gccttagcat
    11341 aactaatatc atctattact attatagaac cgtattttat agtgaatatt gatgataaag
    11401 caacttccat catagggttt gaattagata atctatttgc aaatactatt gaaggatatc
    11461 caaatgttct atcttgctct tttaaagctg ttcttctaac ttgtatagca ttagtgtaag
    11521 tatcttttat gttttctcct gttacatcta atactaattc tttatatcca gcggcttgaa
    11581 ttaattcaac tgtttcatat aattcttcta agctaccagc ttttacacct aatggtaaac
    11641 ttgctccttt aactacttct atcatatctt tgtagttctc tttagttgct ccatatacca
    11701 ttggtctagc atcttttaat acttcaacag cttctttaac tacttgtgca tcatcacaag
    11761 ctaaaatata agctacttct aatccactgc cttttatttt atctattaat tttaaatacg
    11821 catctttatc tccacaatac tctaaaacag ccatttcaac tttcatttgt tcaccaattc
    11881 ttacatagtc aacttttttc atgttagcta ttttagcatc tactgcttca tcactcatac
    11941 aagtacagaa tgaaactgca tatctatttc tacttactaa tgttttttca tgtctaaata
    12001 atactgtttc tccacctagt tcatactcag atgcaccagc accaactttt aaagctttca
    12061 ttaaaggtgc agtagcctct gataattttg ctatagcgtc atcagacata tgtgggcatt
    12121 tacctacttc tacagctcct gaagcaacct tcatagagaa agccatacaa gttgggaatc
    12181 cacaatcctt acagtttttc tttggtgtta atttaaatat atctaaagct tttaatgcca
    12241 ttattttttc cccctaacct taacaattta attaagctaa tgcgttaacc aattctttta
    12301 tagtttctat tgattttgga tgacgaagta taactgcgtt tgaaccacct actaaaacac
    12361 ttgctgcagt tgaaacttcc attgctatag ttctttcttc tggacaaccc caatctggct
    12421 catcttctat tggagctata gcttctttaa catgacctac ttcaaaagct actggtgtta
    12481 tgataggcat ttgtaatgtc ttatcatttt gaccaaatgc tgcaagtcta attctatcca
    12541 tagtagatgc aacgtactca tatccataac caactgctga acatccaaca ttcataacta
    12601 tattttcacc tttaacgcct agttgagtta acaatacgtt taattgttta gctaggttta
    12661 tatcaacaga agattcagct cctactttat gtgcataagc cataccagct gatgctccta
    12721 ctcctttgta gttatcttct actgctgaca taaataagca gttatgtcca tccaatgttt
    12781 gagctaattt ttcaaataat ttaccatctt tttcatgatt tcctgaacca gctacaacta
    12841 aaggtaattt tattgcttca attactgcct tagctacatc agcacattca tcaactgact
    12901 tatctaatcc atttgggtca gaaccatcaa attttaaaca aataaaatct gcttgtgtat
    12961 tagcttcaac atatttagcc cactctactg gacaattagc tacatctttg tataattcct
    13021 tatatgaatc agtccagctt tcaggataaa cgtctgatat ttgtatacct atttttggag
    13081 aattacctac ttctccatca aaactataaa aaggtaatac attttcccca cctaatttaa
    13141 ttgctttttc ccctattcct acttcaactt ctgatatttt tccagaatat ttttgagtag
    13201 acattttaaa tgccatattt ttttccccct taagcctatt ttaattctaa tttagcaaaa
    13261 atttctttca tagccacttt agattttgaa tcttcaggta aattaactaa aggaatacct
    13321 gtagaatcat attcatatat caattcatcc ataggtacaa caccgattag ctctaggtta
    13381 tgcttttcaa tttcttcttt aacaccatca ttcataaccc catttggaac tttatttaca
    13441 atcaaaagta ttcttccaac acttaacttt agttcctcag ctaaatctct tattctagca
    13501 acagcttgta tgctacgtct tgagcaatca ctaaccaata aaagtgtatc aacatgtcta
    13561 gtgacttttc tacttagatg ttccatacca gcttcgttgt ccatgactaa gtatttgtaa
    13621 tgaccagata ttttgtttac ttgttctcta agtatcccat ttacaaaaca gtaacatcct
    13681 tcaccttctg acctacccat tactaataag tcatatcctt cgccttcttc gattatagaa
    13741 tttaatctaa attgaagata ttgtgccttt gtcataccac cagggaatgc attgcctaac
    13801 ttttctcttt gatttacttc ttctcttatt tctcctattg ttgcttcaac ttcgatacct
    13861 aacacttcat ttatgttagc attggcatca gcatctacaa ctaatactgg tccctttttg
    13921 tccttaacca aataatcaat taaaagccct gtgagacttg ttttaccagt tccacctttt
    13981 cctgcaacag ctatattgta tcccatttta agacttcccc cttaaatcta gccacagagc
    14041 cttatgtact ctgtctgtct attataattt aggtacagaa tgaacacatt ctccatgtac
    14101 atcatgaagt gcttccatta gcccttcaga taaacttgga tgtggatgta ttgcatcccc
    14161 tacttgttct actgttaatc ctagatgaac agcaagtgac aactctgtca ataaatctgt
    14221 agcatgaggt cctactactg ctgcacctat tatcttatca gtttctttgt cagctataac
    14281 ttttacaaat ccttggaaat gtcctatagc ttgtgctttt ccaagacctc taaaatcaaa
    14341 ttgacctaca ttgtattcta caccttcagc ttcaagttgc ttttcagtct taccaacacc
    14401 agcaacttca ggttcagtat aaacacatct tggaattgct ctataatcta ctaccttagt
    14461 cttacctaaa gcattttcaa ctgctacgat accttcttta gaagcaacat gtgctaggaa
    14521 aggagtatct attatatcgc ctattgcgta tataccttct acattagttt caagatgttc
    14581 attaactact acttttcctc tttccatttc aattcctata tcttctactc cagaattgtc
    14641 aaggttaggt cttctaccaa cacatactaa tgcatattgt gcttctataa cctttccatt
    14701 agaaagagtt gcaaccgcct taccatcaac tacttcacaa gtttgtactc caatacctgt
    14761 aataacctta attttatctt tcttaaattg tctaagtagt tgtttagcta catctttgtc
    14821 ttcatttaat agtatttggt caaccatctc aactatagtt acttctgttc ctaaggctct
    14881 aaagaactgc cctatctcac atcctataac tccaccacct actattaaca tagactcagg
    14941 tatctcttca agtccaagaa cttcatcact agttattaca acttttccat catatgggaa
    15001 cattcttggt acaactggaa cagaaccatt tgctagtatt atcttatctg cttttatagt
    15061 ttctactgtt ccatcatctt tagttacttc tatagtgttt ttatcaatta attttccaaa
    15121 accattaact aagtttactc ctcttttttc aaataagaac tctataccac ttattaattg
    15181 gtttacaact ttattttttc tttccattat ggcagtgaag tttggcttaa ctgtaccatc
    15241 tatttctata ccaaaatcct ttgcttcttt tacagtattc aatactcctg aagaagcaag
    15301 aagtgcttta gttggtatac atcctgcatt taagcaagtt cctccaactc ttcttttttc
    15361 aataaccgtt acatctgcac caagcataga agcttttata gctgctacat atcctcctgg
    15421 tccaccacct actactacta tcttcatcct taattccccc aaacctaata taatctacaa
    15481 tcttgcaata ctataaacct gcggcatcca gtatagcagg tatattctct tctgccccaa
    15541 ttgccattat atgaacacca tcacataaat cttcttcttt taattgtttg ataagttccc
    15601 ctgccatttt tattccttca cttacccagt tttcttttcc agctgctctt aatctttcga
    15661 tttgttcatc tggaacgaat attcctggta catttgcagt cataaattta gccattcctg
    15721 gtgattttaa aggtactatt cctgctaata ccttgcaatc catatccctt gttaattctc
    15781 taaactttct cattgtattt atgtcataaa gtgcttgtgt ttggaaaaat ttagcccctg
    15841 cttttatctt cttttgcatt tttaataatt gaacttctat tggagaatat tctggagtta
    15901 ctgatgctcc cagataaaaa tctggagaac cttttaactt attaccagcc atgtccgtac
    15961 ctgccattaa agtctcagca gtttgaagta tccccacaga atctaaatca aacactccct
    16021 tagcctgtgg atggtctcct acgctagtat ggtctcctgt tagtgccaac aaattgttta
    16081 tcccaaatac acctgctgac aacatttctc cttgtatagc tattctattt ctatctctcc
    16141 ctgttatttg aataactggc tctaatccag catcttttaa taatttacat gtagcaagag
    16201 aagttgctct cataactgcc gattgaaagt ctgttacatt tgctgcatga actcttccaa
    16261 ctaacggctt tgcacattct attaaatgtg aaaggtcagt cccttttgga ggagccattt
    16321 cagtagtaac tgcaaatttt ccactttcta atgtttctct taataagctc atctctaatc
    16381 ccccttttaa ccttctaaaa aaaactttta tgaatttgct gctgcttctt tctttttctt
    16441 tgcacttaaa cttcttggat tattttgctt agaatagtct tttggtggtc ttatttctgc
    16501 taagttatct aattgtccta tagcttctag tctttcatat atctttatcc aagcacaatc
    16561 attctctgag tttacttcac acttaccatt tttagctcca ccacaagccc cattcataag
    16621 acctttagca cacatagtta ctggacatat cccacctgtc caaccaagtt cacagtctcc
    16681 acaagcctta caggcttctt catattctcc aactctttgt acttccccta taaacaaagt
    16741 attgttggct ggataaactg gcttatcttt taaattctta actattgtct gtgtaccatc
    16801 tccacatgct aaagataaaa ctgcatcagc ttccttagtc tcttctttta gagcctttaa
    16861 atccttttta gactttaaaa ggttacaagc agggtcaagc attacatatc ctaagatttt
    16921 tttgccttca ccttcaagag tttccttcat ttttaaaact tcttcttccc caccactttt
    16981 acaagttgct gcacattgat tacaaccaac taaaaccagc ttgtcaaaat cctttaagta
    17041 ccctagcact tcttctaatg gtttattttc agaaattatc atatctactt cccccctaaa
    17101 ccttccacat atatatctct atttattgtt ttgtaattta caagccttaa ctacatgttt
    17161 tgcaagtata gatgttgtaa ctgaaccaac ccccgctgga actggagtta tcattgatac
    17221 tttgtctaaa actgcatttg tatcaacatc tccacataaa tttccttctt catcaacatt
    17281 tataccaacg tctataacta ccgctccatc tttaacaaaa ctttcatcaa ccatcttagc
    17341 tctaccaata gctgctatta gcacgtctgc ctctgctgct acacctgata gatttttagt
    17401 ttttgaatga catattgtaa cagttgcatg ttcacttaat aaaagcattg atactggctt
    17461 tccaactacc attgatcttc caagaactgt taccttactt ccttttaaat caacattata
    17521 gtgttttaat atctcaacta ctgctgtagg tgtacatggt ggaaatccac ttttatcccc
    17581 ttccattacc ttagcagagt ttattggact aaaacaatct acgtcttttt ccggtgcaat
    17641 aacatatttt ataacttctt catttaagtg ttttggaaga ggtctaaaac ataatatacc
    17701 attaacattt ttgtcatcat taactctctt taatacatca atatattcct cttgtgttat
    17761 atcctcagct aactctaaca cttctgttgt tatacctatg ttttgacatc tctttagtgc
    17821 tcctctttca taagataaat catcacttct tgccccaact ctaactattg ttagtttagg
    17881 atttatacct tctttaacta gtaaatcgac ttcttttatt aattcttcac taattttatc
    17941 agctacaggt tttcctttta taatttgtcc tttagttgac attccttcca tacattcaca
    18001 actcccctac aagcaaactt aaattaattt attctatata ccacatatta ataacatagt
    18061 atctactttc gtcatttaaa aattattttc caagagcttt ttcaactttg gcataaactt
    18121 catcacaaat ttttacacca tcttcaacta acttatctac ttctattttt accttattaa
    18181 catattcttt atcttgtata gaatttatat ttataactac atttaattgt ccaccaagta
    18241 tagcagctct taaacactga actcctacac caacatcact tatagcaagt cttgaacctt
    18301 tatcaactaa atcttcatgt aacttaatag cttcatagca aactcttact atgtttaatg
    18361 gtacttcaca agcaactttt aatgcctttt ccattgtttc tgcttttatt cttttttctt
    18421 cttctgtttc tttaggaagc ccgtaagctt ttgataatgg taagaaacat tctgcatcat
    18481 catctatcat ttttaataaa tctttttcta actttcctgc cttacttaat atctctttta
    18541 cgctttcttc atactcagca tacttcttct tccctattgt aaggttacat accatactac
    18601 ctaaagccat acctattgca cctactagtg cagcagctcc cccaccacct ggtactgctt
    18661 ctttggaaga tagtacttct acaaaatcaa cacaagtttt atctgctatt ttcatgataa
    18721 tcctccttga aaggatgtag gaaaatatct caaattattc gagatatttt acgaactatt
    18781 ttaagatatc tcctactctt tatttataaa ttaatttcct tagaataatc cagctattac
    18841 tccattttca tcaacatcta ttttttctgc tgctggaact tttggtaatc ctggcatctt
    18901 cattatttct ccagtaagtg caactataaa tccagctcct gctgaaattg ttgcttgtct
    18961 tacagttatt ctaaatcctg ttggtctacc taatttagtt tggtcatcag ttaaagaata
    19021 ttgagtctta gccatacata ctggaacttt agtaaatcca agtttttcta agttagctat
    19081 ttccttatca gcttcaggag tataatctac accatctgct ccataaatct tagtagctat
    19141 agcatttatc ttgtctttta tagataaatc atcttcatag cagaatctaa agttattttc
    19201 ttcttcatcc aataatctta aaacttcttt agctactgca attccacctt ctccaccttt
    19261 tgcccatact tcagaaagag caacattaac acctaattcc ttacacttgt cttcaacaag
    19321 tttaagttca gcttcagtat caagtgggaa tctattgatt gcaacaactg ctggtaatcc
    19381 atatacttga gttatgtttt caacatgttt taataagttt ggaagtcctt tttcaagagc
    19441 ttctaggttt tcgttattta agtctgcttt agcaactcca ccattgtatt ttaatgctct
    19501 tacagtagca actattataa cagcatctgg ttttaagttt gccattctac attttatatc
    19561 taagaatttt tctgctccaa ggtcagcacc gaaaccagcc tcagttatta catagtctgc
    19621 aaagtgcata gccattcttg tagctataac agagttacat ccatgagcta tatttgcgaa
    19681 tggtccaccg tgtacaaatg atggtgttcc ttcaagagtt tgaactaggt ttggttttaa
    19741 agcatctttt aataatgcag ccatagcacc attagctttt aattgtctag ctgttacagg
    19801 ttcaccttca tagctgtatc caactattat gtttcctaat ctttctttta aatcagatat
    19861 atcacttgcc aaacagaacg ctgccattat ttcagaagca acagttatat cgaatccatc
    19921 ttgtctaact gctccatcac cttttttacc cataccatca acgatatttc taagttgtct
    19981 atcgttcatg tccacacatc ttctccaagt tattttctta gggtctattc taagtgcatt
    20041 tccttggtga atatggttat caagcatagc agctaaaaga ttgttagcag ctcctatagc
    20101 gtgaaaatct cctgtgaagt gtaagtttat atcttccata ggaacaactt gtgcatatcc
    20161 tccaccagct gctccacctt taacaccaaa tactggtcct agagatggtt ctcttaaagc
    20221 aactaataca tttttgtcta gtttagcaaa tgcatcagca acacctatag tagtagtagt
    20281 ttttccttca cctgctggag ttggatttat agctgtagtt agtattaact tagctttttt
    20341 accagtctct ctttttagta gattgtagtc aacttttgct ttgtatttac cataaagttc
    20401 tacatcatct tcacctagtc ctaatttttt tgcaacctct ctaatatctt gaggcttagc
    20461 ttcttgagct atttcaatgt cagatttaaa tcccataact gaatctcctc ccacaaccta
    20521 aatagattta aaatataatt tttgtaaaaa ttgtattaat atgtaaaaat tatattttat
    20581 aagataatta tacatgtaaa attacttaaa aactagctac taagctatta aaatattcaa
    20641 acttaatagc tagttattta tctaattact tacttattac ctatctattg cctatgtcta
    20701 acctttaacc aacttaaaat cccaaatcaa aatttctatc tattttattt atccttaagt
    20761 gacattaatt tatcttttat tgctttaatc tgttctttcg ttgtttcact agaatcatat
    20821 ggagattgat tagctctatc agaatcaata acatcttggt tatagtaaat aaatcctaaa
    20881 gattctccat cttctaagtt ttggattata aattcttcat cttccttatt tctaatttta
    20941 tttcctacta caaatacttt atttacacct atatcatgtc caagtttctt aacttttcta
    21001 taagtctgta gacttctctc tcctggttct acaactacta taaaagcatc tacaccttgg
    21061 gctgttcctc ttcctaagtg ttcaattccc gcttccatat ccataaccac tacatcttta
    21121 ttttgtagta ttaaatgtga gcagagtctc ttcaaaagaa catgctctgg acatacacat
    21181 ccagtaccac cagaatctac ggttcccaat gtaagtaatc ttactccatt gtattcttta
    21241 cagaaatttt cagggatatc gtctacttta ggattcatct taaacatttt tccaaatgaa
    21301 cctacagaag cagctgttct atctgaaact aattttttca tttctgatat aggtactatt
    21361 gattcatata cttcttttgg aaaacctagt gctaaagcta aattagcatc agggtcggca
    21421 tcaacagcaa caactctgta tccgtcttca gcaaacatcc tagagagcat tgaagaaaaa
    21481 gtcgttttac ctactccacc tttacctgtt attgctatct tcataatccc taccaccttt
    21541 tattactatt tgctttttag tagttaattt tacagttaaa tattttttat actctatatt
    21601 tttatacact aaaaataaac tacagctagt ccttacagga accaattaaa tttaccctac
    21661 tagcttttca aagcagcatg tatagtttta ttattcttta atattgttta tattttattt
    21721 attcagtata ttcattatat ttcgatgttc tttactttaa tatttgactt tattcaactt
    21781 tactcaacca gaaacttctt ataaaaacgt ttattctata acttcactat tttttaagct
    21841 tcagcctttt ctgccatttt ttcttcaact aatgcttcaa agtgaacacg ttttttctct
    21901 atatcttcta ttattgtagc tgctaactct tttggatttg tatttacagt aaatgtagct
    21961 ccaactttat cttttaactc actagttaat atatccactg ctcttgaaga accagctatt
    22021 ggaggcatta ttcctaaata agtgtctata ccagaagcta caacatagca tcctatagca
    22081 actgcttttt ctgacatcca ttctggagct atacctacaa ctggaaggtc acacatatcc
    22141 atatctaagt aattagctgc tgctccaact aaatctagta tacgacttat atcaacacaa
    22201 gaacccatat gtagtactgg tgggattcct actaatttac agacagttgc aagaccttta
    22261 cctgcatatt tttcagccgc atcagattcc attaaaccaa acttagctgc tgctgaagct
    22321 ccacaacctg tagtaactac tattatgtcg tttttgatta attctttcat tattgttacg
    22381 tgagcctcat ttgaaactcc tttagcattg ttacatccaa ctacaccagc tgctcctctt
    22441 aatactccag cttttagaca gtctgttaat ggcttaactg ttcctgctgg gtctatatga
    22501 gagtttacaa ctctgtctaa ttgatttact actgcttcta cactataccc aactgttgcg
    22561 cctgatttta attcaggtat aagtactttt gatttatctc tatttttaaa gtttaaaata
    22621 gcttctttaa ctattgcttt agcatcatca agtgcttgtt cttctctaaa ttcaatataa
    22681 gttgaacctg ttatcttagc ttttggagaa gttgttacaa atttagtatg gtaacaatct
    22741 gcaactctag ctaatgcagg gaatatacat tgaacgtcaa ctattacagc ttcaactgct
    22801 ccagttatta ttgcaagttc ttgttgatgg aagtctcctg ctatttttac accatgtctc
    22861 attgtaatct cattacctgt acaacacata cctgctaagt ttattccatc agcaccaact
    22921 tctttagcta aagctactaa gtctggctct tctgatgcta aaactatcat ttctgataat
    22981 gctggctcat gtccatgtaa tattatgtta actttatttt cttctaatac tgctaaatta
    23041 gcctctgttc ttcttggcac tggagttcca aataagatat cgcttagacg agtacctatc
    23101 atagaaccac cccaaccatc agccatagaa gttctaagac tcatgtgtat taaactatct
    23161 atatcaccag tacatcctat atgagtagag tgcattattg ttgcaatttc tctatctata
    23221 gctcttggct ctatagcgta ttctttccat attttttgtc ttggttctgg tgcatttttt
    23281 ataaatctta atgtcccaaa aggttttcca aattccatta atgcaatttc tgccacttca
    23341 tgtgctacat catagatatc tctaccttct gtctctacat cccattcttt agctaaagta
    23401 attaacttag cttcgtctct tattttatag tttccatttg gatcagctaa tgctaatgta
    23461 tgtgctatat ctctagcatg gtctgaatgg gcagctgtcc caccagcaac cattcttgca
    23521 aagtttctac ctactatagt atgctcatct gctccacata acccccttgg agtcttaggg
    23581 cttattctac atggtcccat tccacatatt ctacagcaaa caccttgaag accaaaacca
    23641 cattgtgcct taaagccttc ttttctatca tacattgttt ctacgccttc ttctctagct
    23701 ttagctatca tcttctggct gtttaaatca attgttaaca ttttttcatc catattccta
    23761 tccccttttt ttgattttta aaatccccca ggtgactgac ttcatgtcat aaaataataa
    23821 aataaatgct ttctaagttg attatatact aattttcagt gctttgcttt agatttaaat
    23881 atttttttaa caaaattaga taatacataa aaattttatt ttctacactt aatttaagtt
    23941 gattttatcg cttgtttttg gctatttgtt aatatctaaa cctacttttt tgatacttca
    24001 gtttaattaa attatgtttt gattttttta aatcaaattt gaaatttttc tagtcatttt
    24061 tatattattg cagattgttt gtgtatttat tatctttcac tactgtttgg acttattaca
    24121 gatttttact ccctaaactt aattagtaaa aacttcttag aatagcattt tttggactaa
    24181 tttttttgaa tttttttatt tttcctactt taatattaat tatagcttca aattgaacat
    24241 ttgtatatat tcatttgaag ctataattat tttttatata aaactttttt aatacagcat
    24301 taatttagac attttgacta ttttttctac tttaattcat ttatttcaag cgatttatcc
    24361 tatttcatat ttttagctac aatagcccca tatgcaatag aatataattt agattcatgt
    24421 gaatcagctc ttgacactaa aactatagga gcttttgcac ccataattat acctgctgat
    24481 ttagagtctg ccatatatgt aaaggctttc cctattccat tgccaacctc tattgttgga
    24541 accaatatga tatctacatc tccagatact tcacttttaa acccttttat ctcactagct
    24601 tcttttgaaa cagctaaatc aaaagccaga ggaccttcta caattacatt ttcaccaaat
    24661 tctccttctt tacaagcttt tgctaacata tctgcatcta cagttgcttg catcttagga
    24721 tttactttct ctttagctgc caaacatgca acttttacag tttccattcc taaagctttt
    24781 gcagctttta tagaattttt taatatcttt gccttttgtt cataatcagg agctatattc
    24841 atacctccat ctgtagttat aagtaattta tggtattttt ctaactcata taccataaca
    24901 tgacttaata aactatcagt tctaagacca tagtctttat ttaacacttc ttttaatatg
    24961 acagatgtat ctaataatcc tttcattaca aagtcggctt ctttacttga tactaattta
    25021 actgctattt ctgcacactc tttgatatca tccttatcca ctattttgat accacttaaa
    25081 tcaaatccta tttcttttga tatttctctt attttgcctt catttccaac caatatagga
    25141 gtaattatat ttctttctac agcatccttt attgccatta aaacttcttt atcatgagct
    25201 gctgctacag agagtacaat tttctctcct cccttaagtt gttctaaaat atcatctaat
    25261 ttttttatca aaagtaaccc ctcccttata tatatttaac taaataatac catatttgct
    25321 ataccttttc tcaatttatt ttttatgtta taaagaattt atatgactaa aagacagttt
    25381 aatattttat tctgggattt catcaatagc tcttaaaagc tttgcaggaa caccacctac
    25441 aatagtattt tttgttacaa ctgctccagc agcgataatt gcattatctc caatagtaac
    25501 tcctggaact cttcatacaa aaaagaggct tatctctatt ttgagatagc cccacttaac
    25561 cgataaaatt aaatacaacc taacaatatc cttacttatt tattccaagt tctcttagac
    25621 aagcaatagt aatttcatct tgtggattta attcataagc tctttctata tagtaagttg
    25681 catcatcaat atcaccatta tttaaataag ccattcctaa attgcaaagt atttctgggt
    25741 cttcctttat cttagccgct tgttcaaaat actctattgc tttatctaaa ttattcttat
    25801 aagcctcaca aagccccaat tccactatag tatccacctg attaggcttt attatcaata
    25861 tcttctcaag atacatctta gcctgctcta tttcgcccat acccttatat ccaagtgcta
    25921 tcataaacag caagttccac caatccatat gttcttcttc taatggaagt aacttctcta
    25981 atccttcctt aaacttacct tggaatacaa gtgtatatcc ttcttcatat tgaactttaa
    26041 agtccatctt gcctagattc tcttgtacct cagctactaa atcagcatct aaccctagcc
    26101 ttaatgcttc ttcccaagtc agcttagtct ttaaatattg accttgatta taataatggt
    26161 agcctaagtg ataataacct aatgcaaaat tctcatcaac atcaattacc ttttcaagct
    26221 tagccatggc ttcaagtaag aatgcattca ttgccttatc atccatatct ttttgatact
    26281 gtttagctat ctcttggcaa acaatcgcat agttatacat tgcatctaaa tcatctggat
    26341 acattgttat aaatgctttt atatatatta aagaatctgt atgttcacca atctcaaagt
    26401 attttctcga catatatccc atatacgatt tcaaatccaa atcaatatct tttgataaag
    26461 catttaaaaa cttctcatac tctgcattgt ttttaaactt actatctatt ccaattatat
    26521 atatcattgc atctgctata gacattgaat ttaacttatc ttgagcagtc ttttctttta
    26581 tcccttttac taatacctca ttttttatag gtacatctaa tccgcctttt ggcactttat
    26641 agctttttaa tttaaactct ccaccatgtt ttatagttat aaaagctaat tcttctgctt
    26701 tttttagcaa atatttttct atcttaaaat ccattttatc ctcctaaata cttaaatctc
    26761 tactttatga gttttagatt tctcaaatat ttttttatca tcatactcaa ataagactac
    26821 attttctact tctcttattt ctgtatttat atcatttttt aaatccacaa cattaaatct
    26881 aaatggctca aaatgaacat attttattat atgtttagct ggtttatcaa caaaactagg
    26941 taaatatgtt gataagttat cattatcttg taaaaataca tggcatctat tcttaaaatc
    27001 atccacatct agcttattaa aaaactgtgg tatatttgat gttttaccca gtgatatata
    27061 gtcatatttt aatatatcat tgaatacatc attatctatg ttgatttttt cattatagaa
    27121 atccatcaat attttatata actgattctt tccttgtgcc aaatcaaagt agccattttc
    27181 atcaaaatac ttggcaaaaa cctcaaagaa tttaaaagga ctttgtttat aaaatctgcc
    27241 tataatatat ctcatagata acacaaagtt tttagaattg tagtatctct ccagtatatc
    27301 ttctatatcc tttagcttca aagtttcttc atagcttata taattattgt ataaaaactc
    27361 atatggagca taatccttat atttaaaacc atgctcatct gcaacttttc tcatacctgt
    27421 ccctttaatc atctttaaaa agcctaattg cagatgctct atacctagat taaatacatc
    27481 attaaaagaa ttttcaaagc tcttataatc ttcataaggc agtcctgcaa ttaagtctaa
    27541 gtgctgatgt atatttctat aagatgctac agtttgtaca acatgagaca actttttaaa
    27601 atcatccctt ctacctactg catcaagtgt tttttcattc gttgactgaa cccctatttc
    27661 aaattgaaaa agaccttctt tacaatcagc caaaaactca agcatcttat catctatcaa
    27721 atacgctgta acttcaaaat gataagttgt atatccatta tcatttttca taaggaaatc
    27781 catgatttcc atagccactt ttctatttgc attgaatgtc ctatctataa actttatctg
    27841 tgatactctt gcatctatta aagcttttaa atcactcttt atcctgtcta aactaaaata
    27901 ccttagacct ggaatagtag atgataaaca gtactggcag ttaaaaggac aacctcttga
    27961 tgattcataa tacactattc tattttcata ttcttttgga ttcaagtttt cataaggact
    28021 aggaatctca tccaaatctt gaagtaattc cataggctta ttcactatta tctcattgcc
    28081 ttgtctatat acaagtccat ttacatcatt tattttcatc tttccttgca agtgaagtac
    28141 taagtctcta aaaattaatt ctccctctcc ataaagtata taatccacaa aatcatactt
    28201 tttcattgcc tcataactat cataactcac ttccggtccg cccaaagcta ttttagtatt
    28261 aggtctaatt ttttttagat tatcacaaag tttaactata tctcccacat tccagatata
    28321 tgttgagaat aatattatat catattcatt tttatatata tcctttaaaa tatagtccaa
    28381 atcgttgttt atcgtgtatt ctttcatatt aacttctatc aaatctctta caaattcttt
    28441 tagatatctt atagccaaat ttgtatgaat aaacttggaa tttaatgttg ttaataaaat
    28501 tttcaaatat ttctcacctc tttttaagat tatactgtct ttttgtaccc cttataggtt
    28561 aaaatagtat atgtaaagtc taaataatat ctatataatt aatttattaa tttcaagtaa
    28621 tgtgaggaaa ggaagttttt tatggaatta attgaaaaag tatctatcaa ctctttatca
    28681 aaaagagatt tactacttat tataaagtct cttgaataca ctaatgaaaa tactcaaatt
    28741 agtgatttca ttgaccttag gaataatata gtaaaggaat tatgtttttt aactgacact
    28801 aaggaaaaag attttttaga ttatctagaa aaaaatagca cactctaata acctaaacct
    28861 ttttaaactt taatatataa atacctgatt tagaatttat ttttatgctt aatatattat
    28921 cattaaaggt tttcgacata actgttattt gattaaatat agggtctatt atagtattac
    28981 cttcttcaag tcttataaga cttcttgggt ctatgctttt attatatgcc ttaactccaa
    29041 ttttatattt tctcaattta acaacatatt cggacataga aaaaataaaa tctatattat
    29101 cttcatttaa tttactatct tcacccaaaa tggataatat agattttttt atgctattaa
    29161 ttccttcatt ttctacagtt ctatcttcca tatatacatc atatagaaca tttaaaacat
    29221 ttaataaaat actttcttca tcataatctt cagatttcaa atcaatgtac ttatcaatta
    29281 attgtattgc tttaatcctt gccatctgaa actttacaac atctgatttg tctaattcta
    29341 tagaaaatcc tattatcttg tctttaatac tctgtagtaa ttcgacatac tctattttac
    29401 tattatttat aagattgtgc ataatggaat tatacactac agaacctaat aaataatcaa
    29461 ataaaagttc ttctttttta aaataaataa catacttttt tattagttta ataacctcat
    29521 cttcgctttt ttgaatatta gtatttgata aaattagtgg tagtaaaata tttaaaaaat
    29581 tgatatttcc tattttttct ccataaaatc tatagtaaat aagttcatct ctcagtctat
    29641 tttcgtctat atacacacat ctttgcgttt tatcatctat taaggatttt gtatttatta
    29701 tagttaaatc tttaagagcc ctacttgcac tactaaaaga agatgtgtag acttttttta
    29761 gtaattcgtc attcataaat atatcctttc tgaaaaattg aatgtaaata aattgttatt
    29821 aaaaaaaaag agacagcccg tttaagaagc tgtcatatat agggggatag tagtgtacaa
    29881 aaatcattgt aatctactat ctatatatta ttatacgata ctactttttt atatgtaaag
    29941 catcagtttt cgacatccct atattttaca gttatacaaa ttgcgtacta agttcaattt
    30001 tattcttttt atttcaacat tttatcatgc tacattcttt ccggagcgtg cattcccaac
    30061 aatttcagag aattttctaa agtttgtttt gttgctaaaa ctaatgcaag tcttgctttt
    30121 cttatttcca aattttctac taaaattgga ttgtcatgat aaaatttatt aaacgcttga
    30181 gctaaatcta acataaatct agtcactata tgtggctcat ttttctttag agcaagtaaa
    30241 atattcttat tgaatgattc tataactcta agaacttctg cactatcttc atcagctaat
    30301 agactataat ctatatcaga agtcacttct acttctgctt ttcttaaaac tgcacagcat
    30361 ctagcatgtg tatactgaac atatggtcca gtctcaccat caaaactaag cgttctttcc
    30421 caagagaagg tatagtcttt aattctactg ttagataact cttggaatac tactgcccca
    30481 acacctactt gcttagatat ttcatccaca tttttagcat ttggattttt agctagtata
    30541 atttccttag ttttatctat agcttgattt aaagcatctt ctaaaaatac tactcttcct
    30601 tttctagtcg acatagtacc ttcttcaagt gcaaccatac caaatccaac atgaattaaa
    30661 tcttttgccc aatcatatcc cataagctca attaccttaa accattgttg gaaatgtaag
    30721 ttttgttgag aaccaactac atatatacac ttgtcaaaat cataagtctc ttttctatat
    30781 attgcagcag ctaaatctct tgtcatatat aacgttgaac catcattctt agtaataagt
    30841 gctggtggca ttttatactc ttctaaatca acaattcttg ctcctttaga ctcttttaaa
    30901 agatttttct cttctaatag ttcgataact ctgttcattt tatcagaata aaaactctct
    30961 cctgctaaag aatcaaattc tatatctaaa agtttataca ctctgttaaa ctcttttaaa
    31021 ctctcatttc taaaccattg ccataattct tgtgcttctt cgtcaccatt ttctaattta
    31081 ttaaaccatg ctcttgcttc atcttccatt tctgggtgtt gttcagcttc atcatggaat
    31141 cttacgtata attttagtaa ttctggaatt ggattagact ctactacttc tttttctccc
    31201 cactttttaa aggccactat taacttacca aactgagttc catagtcacc taaatgattt
    31261 attcttatag tcttgtatcc ttgcgaatca tatatcttat ataaagcatt acctataact
    31321 gtagttctta tgtgccctat atggaaaggt tttgctatat ttggagaaga atattcaact
    31381 attacagttt tatcttttcc aaaatcacta tgtccgtaat tttctttttc atctaaaact
    31441 tttttaatta cagtttctgc taattgagat ttatttacaa agaaatttac atatccgcct
    31501 aattgtatta cttttgttat ctctccactt ggttctatac tttccgccaa ttcacttgct
    31561 atcatgtttg gagcttttct aaacactttg gctaatttaa agcatggaaa agcatagtcc
    31621 cccatatcct tatttggtgg aacttctata agtgcctcta tctcttcttt cgataaatct
    31681 tctatttttt ctttaaggca atttgatatt gccaccttaa aatcttgcat tctgttgctc
    31741 ctctctacat tgttcctggt ttatattgtg cttatctctt tatgttaaat caaatttcgt
    31801 gttttttcaa gtatttcatt acagtgtaaa acactttaat aattatgttt tctgtatata
    31861 ttaaaaatac tataattgca gtttttaatc acactattat tgatttttta taagactttt
    31921 ttctatttta ttgtttcttg tactatccag ttgactttat atactttttt taatctttta
    31981 tcaattttca tacatcaata ttaattttat atacatttat tttgctcttt tatctatttt
    32041 tatacatcaa tattagcttt atatactttt tttactcttt tatctatttt tatacatcaa
    32101 tattagcttt atatactttt tttactcttt tatctatttt ctataaatct ttttcactct
    32161 taatttttat tccttcttct tctaacagcc ttgctgttat acctttccct ggtatctttt
    32221 tacctgaaaa actaccatca tatattagat tacatccaca agaggggcta ccctctttta
    32281 gtattgcttc gctacataca tataaatttg ctatttttag agtttcatta gcccctctta
    32341 caaagttttc tgttacatcc ttattatctt tagtaattac ttttccatca actatctcag
    32401 caggtggtct tggtgtagat agaccaccta actgctctgg gcatacaagt gtaaattctt
    32461 tgccttttaa atattccttg actttttcat tttcattatt atcgccacta tatttacaat
    32521 ttattcctat taaacatgca gataccaata tcataaaaat gtgctcctat acaaattctt
    32581 aatatattct tatacaaatt actaatgtat tcttatacaa attattttaa tttaacaatt
    32641 gttactcctg ctccaccttc tccatattga ccacctcgtt gtgattttac atgtctattt
    32701 cttcttaaaa tgtcttgtag ccctgctttt aatactcctg tgcctattcc atgtattact
    32761 gttacagact ctaaacctgc cacatatgca tcatcaagat acttttctac ttccattata
    32821 gcttcttcta ggtttaaccc tctaaggtca acttcatttt ttacactacc agattttgac
    32881 cttataatgt ttctcgttga tttagttaca tttgttgaaa catctttgcg tgttttttgt
    32941 aaagatttaa aaggtagagt catcttcatt ataccaattt gcactactgc ttcttttctc
    33001 tttttatcta ctgaaactac actaccattt tgatttaaag ttattacctt aacttcttca
    33061 cctggcttta agtcttttat ctccttattt gatacctttg gaactatcat actttttact
    33121 gttggctgaa gacttcccat agagctggtc aattcttttc taagttcttc tatcttacga
    33181 ttcttttctt ttgaagctct ttcttgttca agatttctaa gttcttttat tataatatct
    33241 acttcttctt tagcttgtct tataattgaa aatgcttctg atttagcttt ttcaatcatc
    33301 ttatctcttt gactgaccaa tttttcaagt ttttcgtcat actctacttt cagtttttca
    33361 atctcttctt ttaatctttc agcttcttct ctatcttcta ctgctttgat tctattcttt
    33421 tcaacattct gtaacacatc ttctaaagct atgttttctg tatttatata ttctttagca
    33481 cgtgaaatca cataatcact tagcccaagt tttctagaaa tctcaaacgc atttgatttt
    33541 ccgggaacac ctataagtaa cttatatgtt gggcttaaag tttctatatc aaattcaact
    33601 gctgcatttt ctacacctgg tttagtaaga gcataatttt taagctcact atagtgagtg
    33661 gtagctatac atttagctcc tacagaattt atatcttcta atactgctat tgcaagggca
    33721 gctccttcaa cagggtctgt tcctgctcct aactcgtcaa atataactaa agagtctgct
    33781 gttacgtttt gtaatataga tactatattc gtcatatgag atgaaaatgt tgatagactc
    33841 tgttcaatac tctgttcatc tcctatatct gcaaatacat tatcatatac acacatacta
    33901 cttccataat cagcaggtat atgaagtcca ctttgtgtca taagtgcaaa tagacctaca
    33961 gtttttattg taacagtctt accacctgtg tttggacctg ttatcaccaa tgtatggaag
    34021 tctcttccta aataaattgt atttgctact accttctttt tatccagcag tggatgtctt
    34081 ccatttttta tgtttaaata tttatcctca tttaaagttg gttctattcc tctcatctgt
    34141 atagaaagtt ttccctttga aaatgcaaag tccaatcttc caagtatctc ttgattagag
    34201 attaaatctt cgctcacttc tccaaccata gctgaaatct ctgataaaat tctttctatt
    34261 tcctcttgct ctcctagttt caactgcctc aattcattgt tcatctctac tatagtcatt
    34321 ggctctataa aaagtgtcgc accagaagat gactggtcat gcactatacc agctacttgc
    34381 gacctatatt cagattttac aggtactaca aatctatcac ctcttagaga aattattgca
    34441 tcttgtaagt acttttgata agtagttgaa cttattattg aatttaattt tgacctaatg
    34501 gattgatttt tttgggctat tcttcttctg atatctctaa gtgtacttga agcattgtca
    34561 gatatttcta cttcactaat tatagcattg tatatctggt cttctatgtc cttgtacaca
    34621 tatagagaat ttgatagaga ttgtattatt ggataattaa aatcttcttc atcactacta
    34681 gataagctat ttcttaatct tctagccact cttaaggtat ctgctatcat aatcagactt
    34741 cctgggtcaa gagaagcacc tatataagct ctctttacct tatcttctat atcatgaata
    34801 ccttccaacc ctactgaacc tctctttata ataattgatt gagcttcact agtttcttct
    34861 agcatagatt tcacttctac aaaatcagta tttggtacta aattttctat atattttaga
    34921 cctaaagaag atgaggcttt cttctttaac aaatctatta ttttattgta ttctagaact
    34981 cttaacgatt tctcattcat tttctatatt caccatcctc gatattttaa cttttttatt
    35041 taattttaaa aatgtatgtt ttcaagcatt tccaataaaa aatgcaagtt ttatttcttt
    35101 ttcatttcta aaattattat attttttctc ttttagatat attttacaca tattttgtat
    35161 tatactatgt atgtagctat ctatatatta tacataaaca tgctttattt gtaatataat
    35221 ctgttaaaaa atcttcttta atatttttaa aggagtttat tttagcttat aaaaaaatag
    35281 ctacttatat agataaatta aggaggaaca tgagtcatgg gaaacgtatt agaaggatta
    35341 aaaccagaaa gtgtttttaa aaactttgag aaaatatctc aaattcctag aggttctgga
    35401 aatgaaaaag gcataagtga ttttttactt agctttggta aaaatttagg tcttgaaact
    35461 attcaggatg aaagcttaaa cataataata agaaagcctg ctacaaaagg atatgaaaat
    35521 tgtccaggtg tagttcttca aggacatatg gatatggttt gcgaaaaaga aaagaatgtt
    35581 gaacatgatt ttcttaaaga ccctataaaa ttaagaattg atggtgacat gatatatgct
    35641 actggaacta ctctaggagc tgacaatggt atagctgttg ctatgggtat ggctatatta
    35701 gaagataata ctttagagca tcctgcacta gaagttttag ttactgttaa tgaagaagat
    35761 ggtatgaatg gtgcagatgc actagaccct agcttaatta aaggtcaata tatcctaaat
    35821 atggactccg aggaggaagg atacttatta gttagctgtg ctggtggtaa aacttgtgtt
    35881 gttagtttac ctgttgaata caaggaagtt aaaggtgaca aacaaggatt attagttgag
    35941 gttactggac tattaggtgg acactctggt atggaaattg ttttacaaag agcaaatgct
    36001 aacaaagcaa tagctagagt attaagtgta ttgaatgttg attatgaatt agcttctgtt
    36061 gatggtggta ctaagcacaa tgctatacct cgtgaagcta aatgtgtaat agctgttaat
    36121 aaagctgatg ttgaatctgc taaaaaacaa ataaatgaca ttttaactgc atttaaacat
    36181 gagtttacta cttctgaccc tggcatgact tactcagtag ctgaaacttc tgtagacaaa
    36241 gttcttacta aagattgtaa agaaaaagtt gttcaaatgt cttgcttaac tcctcatgga
    36301 gttcaatcag taagtcttga tatagaagga ttagttgaaa gttctactaa cttcgctata
    36361 attgaaacta aagaatctac tattgaattt ttaacttctg ttagaagttc tgttatgtct
    36421 attagagatg aaatcgctga tagaataaga cttttggctc aagctttagg tgctaattac
    36481 gaccttatag ctcaataccc agcttgggaa tttaaaaaag gctcaaaatt agaaaaaata
    36541 tgcagtgaaa cttatgagaa attaactggt aaagttccta ctgttatggc tctacatgct
    36601 ggtcttgagt gtggattatt acttgataaa cttcctcatg ctgaagctat ctctatagga
    36661 ccagatatgt ttgatgttca tactccaaat gaacatgtta gtatcccttc tgtagctaat
    36721 gtttgggatt atgttattga aatattaaaa tctatgaacc aatattaaat ttaagttttg
    36781 attctaaata gattttaatt ttaagtaatc tatataaaat taactctgta aattaatact
    36841 actgttagta taatatttac tttcaatatt aacttataga aataaagcct aatgatagat
    36901 aataacatgt atcattaggc tttattgtaa tttaaatatt attaaacttt attctgtttt
    36961 aaaattaatt cttatactat tttaaatcta tttcttcctt gtctcttaga atcataaaga
    37021 gcattatctg ctatttgata cagttcatca tactcttgac cacgctttgt tataactacc
    37081 cccatactaa tagataaact ttctccattt ggcaacttaa tgagatgaat atgattgtat
    37141 acatcattac atttattgat aagttcttcc ttatttatta tatcatttat ataaacacta
    37201 aattcatctc cacctaaacg acctactata tcatcttttg caaaactttc atcaagtacc
    37261 tttgcaacat caatcagtac tttatctcct accaggtgac cataagtatc attaattgac
    37321 ttaaaataat caatatctat aatcaataaa gctccatttt tttcagttga taaatttaat
    37381 aaattttgtg tagttaattt tctaatatat gctgcattat aaatatttgt taaactatcc
    37441 ctttgaactt tatccaacaa tctattcttt tcctcttttt cagaatcaat attagctatt
    37501 ttaccaatta catacattgg tatttttcta tcattataaa ttatttttga agtaattcta
    37561 atccaatgaa aagtatcatc aagcatgata gaatacattt cagtagtccc attttcttgt
    37621 gattttatta catcaaaaaa ctttttatta tattctcttc ttattttatc tctttcattt
    37681 tctatattgc cattttcact ttccttatca cttttatcca aaccataata agtctcaata
    37741 cccttttctg gcatagaaat tatcaattta tcttgcttat aatcatactc aaaaaagtat
    37801 tcacttgtca tttcatacaa ctgagtatgt tttttgagtt ccaaagatat cttttcgttc
    37861 atacttgtac gagtatgtaa accaaatgcc ataaatccaa taatacaaat taataaactt
    37921 gctacaataa aaatagtctc aattggattc tgttgcataa tataaagcaa cgagaaatct
    37981 tgtctatatg tagtattttg gtagacaatt gattgcattt tttctgatga aatacttata
    38041 atagctttat taaatatatt caataattct tgtttatctt ttttaattat gccaaaacaa
    38101 atactctttg cttcataagt ttgaggaact aggcgcacat ttttatactt aggttgattc
    38161 atatagtact gcatagaata accttctcca taagtgtaat ctgcatagcc tttattaaca
    38221 gcttctatac actcttctaa attatcatac caaactatat tttcttcaaa ctgaccataa
    38281 taatcaatac ctttggctag agctatacgc tttccattta gactattctc atttaattta
    38341 ttattaatca tcatagtata ttgagtagaa agaaaaggcc gagacatagc tatattatgt
    38401 tcacgagagt actcataatc ataaatcatt cctgctacaa tatcaatttc atgtttttta
    38461 gccatatctt ccaaatcttc ttgagatttt acaggcacaa gttcaaattc caaacctgtt
    38521 ttatcacata tatgatttaa caaatcaatt gatatacctc ttaattctcc tgtatcttta
    38581 tctgcatttt gaaacggtgg tttgtttaaa agtactccta cttttaattt ttctgtatct
    38641 ttaatgtaat tagtttcttc atcagataaa aacatttgat tattctctgg cgtaaagtat
    38701 ttctcatata aagtagttga aaaatatggg tccttctgtt caatattatt aattgctgaa
    38761 ttaagctttt gagttaagtc tgtactgcct tttggagtaa tgaaataaaa tggctttgat
    38821 gagaattttg ctacagtacg cacaccttct attgggttca tactaacatt aagaagtaca
    38881 tctgctttac cactctttag tacttctaat tgctcgtcac ttgttttaca atataccaat
    38941 tcaggtgaca taagattcat tttacaatac tcatctaatt ctttaagcct agtatcagaa
    39001 ttttctatta cagcaatacg aatttttttc acatcttgcg aactcattat gttatcaata
    39061 tctgtatttt catataatgc ttgtagtact gtatttgcta ctccataact gtttcccgca
    39121 taattatata ttttttgcat tggctcattg tataacatac ctcccattat atcaatttca
    39181 ccattttcta acatcttcat taattttgta atagattcat ttatctctcc aggaacttga
    39241 acaaattcat aattccatcc agtatactgt gcaatttcta gcaaatattc atatgtatac
    39301 ccaaaatagt taccttgctc atctatttca gttaaacctt tttgaatagg ataagcaaca
    39361 cgaatggtac gattatcatt acctatagca ttgataccaa aagaaaatat gcaagaagtg
    39421 aatatagatg taattacaat aattaataaa agtaattttt ttaaattcac tacacaatcc
    39481 tccatatagt taagatttac agtataaatt actgcataaa cttgtagaca tgaaaaaata
    39541 gattagtata taaaaggata tataaatcta cttagaaatt taaatatcct taatatatac
    39601 aatatattta aattataact catttatata tctattacaa caaatttacc ctgctaaata
    39661 cctactctat atcccataat tttacaagtt gtaatgaagt ttaagttcta tctcatttca
    39721 aatttagcag tgtttctatt tacagttctt ttatatttca catctggata acccaatata
    39781 aatgaagtga taactgcttc gttctcgctc attcctaaca gttcttttat ctttggctct
    39841 acatttgatg ccataacaaa aaatccatta taacaaattc ctaatccttg agaataggcc
    39901 ataagttcca tattagatgc agccaatcct ccatctacat aagcacttcc acctagagac
    39961 atatcaccta caactatcat aagtagtggg gcatcataaa ataaagaatc atttccgttc
    40021 tctttatatc ttttgtacat tttcttaaat gtatttttat agatagaacg tacagggtca
    40081 ttgtcatcag tatctaatgc caaattataa agaccttgta ttgccagttc tttcacttct
    40141 tttagctttt cttttactaa tatgtatctt ataggctgtc tattaccacc tgttggtgtg
    40201 tatctaccag cttccactat gttttttatt ttttcttctt ctacctctat atctttatat
    40261 tgtcttatac ttcttctaaa tttaataaaa ttcaataact tttcgggttc taattcaaaa
    40321 ctttcacgat tgtattctat tacttcattt ttatcaaatc cttgcatttc tattgcatct
    40381 gttgggcaaa ttgccataca gtgaccacag ttaatacaga gaatattttt aacctttgct
    40441 ttatcatcct taacttttat tgcatttaca agacagtctg attcacacat cccacagcct
    40501 acacattttt ctaaatttat gtctatcatt ccaaattatt cctcctttta aaaaatttac
    40561 tttagtataa aacaaatagt tgtctaagtc aatttttatt tttttaacaa tatactagcc
    40621 ttaactacac tttaatggag aaaaatatac ataaaattag aacttaaatt agaatttatt
    40681 caaaataaaa gataaaagaa gtaaatgtct aaaagtgaac tttttagtta atgagacatt
    40741 ctttattgta tgaaatcaag tggatattta tcatttcaac aataaaaaag gcttacctct
    40801 attttggata tgtccttgtt aagtagacag actaaaaaag gctatcttta tgcactgaga
    40861 tagccccttt tcccaactac tattgtttat tttaatatta tatcaataaa tttaatttgc
    40921 tataacctgt aagttacatt ttaatttgat gtaactttaa attacatttt taagtaatat
    40981 ctttaaatta tattcttaaa atatatcatt aaactacatt ttaattaatc taaaaactat
    41041 atcttaatta atctaagaaa cttacattat aatcttttag tttcccatta tcatatacaa
    41101 tctttgcact aaagatattt ccagtcttag cccaatttaa gaacaacctg tcaccttccc
    41161 acatatttaa atcgaatatt ttatcattac caacccaaat taaatttcct tctttacaat
    41221 catttaaatc tttttcagac aattcacctt caaactcatc tgagaaatac aaaaacatat
    41281 accaatcttc ttcatcttta aattttggaa aagttatcaa ccctttatat gacaatgatt
    41341 taagagtaag accagtctct tcctttactt ccctaattac acattcttct ggagtctctc
    41401 cttgctcaaa ctttcctcca actcctacat acttaccttc atgtatatca tctttctttt
    41461 tatttctgta caacatcaaa gtcttgtcat ctttttctat ataacaaata gttgtaagtt
    41521 tcatgtatct cgccttactt tctctttata ttctcaatta attattttca gcactttcaa
    41581 gcacatcact tatattcttc attgcattaa gagaaagttc tgcaaattca tcaaatggaa
    41641 tccctatatc ttctatactt ctcattgcat ctctatttac atttgaagca aatctcgttt
    41701 ctttcattct tttaataaca gacttagtct ttacactagt aagtttttta tctggataaa
    41761 ctaaagctat agcagttata aacccagtta tagggtctgc tgcatatatt gctttttcca
    41821 ttaagctagt tcttgtaact cccattccat catgatgacc aagtattgct ctatacattt
    41881 cttcgtcacc gaagttttct tttttaagca gttctactgt tgttgtagca tgtccttcac
    41941 atatttctcc tggctttctt ccaactatat ctgaatccaa atcatgtaaa agacctgcca
    42001 ctgcccactc ttcttctttt tctggctcta gtcttttggc taattctctc ataacagctt
    42061 caacagcata acaatgtttt ctcattctat cagtttctaa atacttaaat aaaatttcta
    42121 aagcttcttc tctcattttc accaatcccc ctgttttttc acatatatga tatttttata
    42181 tattaataca cataaattca agtctttata tatattcatg tatatttttc atgttatatc
    42241 tattctacac ctttatagta gtgtcctgca actgttccaa tttcatctaa tttcttatac
    42301 aaatctgtag ccttttttcc aagtttataa ttattactca atacctcaat gtgagcttca
    42361 ataacctttt taaccatctc actatcttca actgtaaaat ctatcctaaa tacatctatt
    42421 ccaatttcct ctaactcata tagattgtcc aacatacaag taaccttcga attgtaaatt
    42481 gtacttctac agaatatatc ttgtgttatc ctataacttg catccgtaaa gtctcttaaa
    42541 gcataactac tttccttaca tttagcaact ctcttgtcct ttttacaatc tctaacaact
    42601 actcccatag gacagtattc tgtaaccatc ataggagcat atccatatat tactgcttca
    42661 atttctacat ctgtatattt taaagtttgt ttaatttcat ttaagtttag ttcttgtgac
    42721 aaacaaactg tacttgctcc ctcttctttg aagtggttta tactttcact attgaatgca
    42781 tttaagtagc aatctatatt taagtctttt ttcttaaagt attccataga gccccagtta
    42841 ccaatttgaa ccttattaat tccttttata ttatccaata tctcaaattg cttgtactct
    42901 ttatttctaa caatccttgg tgctgaatag attaattttt tgtcagtgac tgctgtatta
    42961 tagtggttct tattcaaagt atctatttta cttgtatttg acaaagtatc tatgttattt
    43021 gtatttgaac ccattagttc tttaggaata cctaaagacg aagaagtatc taaatccata
    43081 gaaatacttg aatctataga aatacttgaa tctatagaaa tacttgaatc tatagaaata
    43141 cttgaggcca tttgtaaagc ttttgataat gtatttatat cttcataata gattaaatct
    43201 acattataat ttaatactgc ttctaattgc tccaagttct taactttaac tctaatctta
    43261 ggaactgtat caagtttttt ctgtttattt actctatttg gttggtattt taatctattc
    43321 ttcttaaatt ttctatcttt tatctttatt ctttcttcat ctaaaagctc aatacaatcc
    43381 cttctcattt gatttaacac acttataggc atacttacac cttcatccag gtcaatttct
    43441 atctctttta acttatatgg tgtatttcca agcttttgaa tttgtgtttc tattttttct
    43501 ttacttaaag ccacctttat agctttttca actattttgt ctccttctac caccgattta
    43561 tttccacaaa aatcacttat aaaaagctct ggctttttac ctagtttaat actcactttt
    43621 gcacgtatat tagtttttat gaactcttta tcttgctgaa atgtagcttg tactctattc
    43681 attaattcgc tgtcagaagt cttatatata agctgatttc tctttgcttc tccaacaaaa
    43741 tctaattcta tcaaatcccc tttatgtgct attgtagtta tctctccatc ctttattatt
    43801 cttccaatag tacctccacc taagttaatt ccatcacctt tcttaagcgt attttctagc
    43861 attactctaa gtcttttgtt ctttctatta taatctaaaa cttttccaat atatagccct
    43921 tgattattag gtctattaga gttcattata tccttaccaa cttcacctaa tatatatcct
    43981 tttgtaaact ttctattaaa tatggtatac aaattattta ttatttcctc agaaatactt
    44041 atttttttct tatctacaaa ttcattaatc gcttctctat atcctgaaac cacagtagct
    44101 acatactctg gttttttcat tctcccttca atttttaaag ataatacacc tgtttcaatt
    44161 attttatcca aatcttctat agtacttaaa tctctaggac tcaacaaata atctccatta
    44221 ctttttacta cttctcctgt atttatatct atcaactcat acttttgtct acatggctgt
    44281 gcacacctac ctctatttcc agacctattg cctatcatac tactcataag gcattgaccc
    44341 gagtaacata cacataaagc tccatgtaca aaaacttcta tatctacagt tgtattttca
    44401 caaatccatt ttatctcatc aacatttaat tctcttgcta aaacaactct atcaaaccct
    44461 acactctgaa ggtattttac atcttctaag gaatgtgcta ccatttgagt gcttgcatgt
    44521 agttcaaagt caggtaatag tttttttata agtcttgcca ttcctatatc ctgtactatt
    44581 aaagcatcaa tatctatatc gtataaaaat tttacatact ctacaaaatc ttctatttca
    44641 tactgtttta tcaatgtatt tacagtaaca aagacttgca ctccccttat atgagagtat
    44701 ttaacagcct caatcagctc atttctatca aagttatttg cagatgctct agcactaaag
    44761 tctttaccac ctaaatacac agcatttgct ccgttttgaa ctgctgcttt taatgaatca
    44821 aaagagccta ctggtgcaag taattctatt tttttcatat ttttctcctc tatttatact
    44881 tgttaggtta atataattat atctataact taaatatttg tattaaattt atatatttct
    44941 tactatattt taaattcttc aattaattta tatattagtt atttatattt ggtccatttg
    45001 agttaacata tacattattt actatattta gtatatttct attaacttat atattgatta
    45061 ctgtatttta aattcttcaa ttaatttata tactgcttac taaatttaaa acattttaat
    45121 taatttgtac actgtttact atacttgaaa tacttctatt aatctatata ttgcctacta
    45181 tacttaagac attttaatta atttatacac tgcttactat acttaaaata cttctattaa
    45241 cttatatatt gcttactata ttttaaattc tttaattaat ctatacaata tttgctatat
    45301 ataaagtata tcaaataaca gcttcatttt tttgtaaatt tacagaaagt taattttcat
    45361 taaatgaatc atctttattg ataaacataa ttccaaagac cactgccaaa ataaaaaaac
    45421 atattagtat tgatttttcc acgaaaattt ttgtaaaaaa agttcctaaa atagctccag
    45481 atataaagaa tataataata gcataatact gtaaactctt ttttatcata aaaatattct
    45541 tagtatgtat ataatgaaat aaagtttctg tagcacttct taaatttcca gtacacattg
    45601 tcgttgcaaa actattccca tttacttttc taaaactctc tacttgcatt gaacaaataa
    45661 aagaaatagc aacatttaca aacatattaa atcttcctct aggaataaat gaaacaataa
    45721 gtattgtaat catttcaatt ataatgataa cctgacgcca atgaatcttt gtcttattat
    45781 ttgtaaaata ctttcttatc ttctcggcta taaatacacc taacataaat gccaaaatag
    45841 gaatcaaata ataaaatgat tcctttacat taccttctgc tatattaagc ccaaataaaa
    45901 ctatatttcc agtttgtgca ttagcaaaga ctttacctct taccacataa gtataaatat
    45961 ccaaaaatcc tcctgttatt gccaacaaag aacataaaaa gaatgattca gacatctgac
    46021 tcttgcttgt catatataaa cctctcgctt tctaatgata gatacaaaat caaaatcatt
    46081 ttagattcta tttttcattg atataaaaaa attatttata tttaaatatt atctatattt
    46141 atacatcatt tatatttata cattatttgt atttatatat cattcatatt tatttatatt
    46201 taaacattat ctatatctat atagcattta tatttattta catttaaaca ttatctatat
    46261 ttatatagca tttatattta tttacattta aacattagct atatttatat agcatttata
    46321 tttatacatt aaaagaagct gtctcaaaaa acaagagaca gcaaatcttt tagtttattc
    46381 cacatttttt gcatcattat ttaaccctgt acattttaac tgtaaatcat aagctttgtt
    46441 ttgaaactca gaagctaagt tttcagctat agtagctctt tcatttgctt cttcaagttg
    46501 agtacttaac tcctcaatct tgtttttata aacatctaac tctgctctag aaccttctgt
    46561 cgtattacta agttcgaata tttcttgctt tttattttct ataatctcaa ttaactcttt
    46621 catcttagtc tcagcttcac gattttctgc ttctttttct gctatcgtaa gctttaaaga
    46681 cttgatttct aattgaagct cttcattaga tgctccaacc ttcttactca attcttcatt
    46741 agctttaatc aacttctcat tctcatctga acattcaaat aaaaggtctg atatatttaa
    46801 tgcagttagt atcgcagcaa ctgataagct caatttcggg ttttgacgtg ttattttatc
    46861 catttctttg tctacaaaat cagcaatact aatcataaac ttctctgatt tgtctccaac
    46921 cattggatat tcagcgccat ggattttaac cattactttg ttcatcctaa cctcccccga
    46981 cattagttgt taatttgatc ttaagttagc atttaatttt tcgcttaatt cacttaatat
    47041 cttatcatgc accttagcta catcttcatc tgttagagtt ttatctttac ttctataagt
    47101 tattgaatac gcaattgact tgtatccagc ttctatttga gaacctttgt aaacatcaaa
    47161 taacttgtag ctttctacta atccttgacc attttccttt attatatctt ctatttgttt
    47221 tacaaataca tcatctttaa caataagagc tatatctctt gatgtagatg gatattttgg
    47281 taatggcaca taattctttg ttctatcaga attatcaaat acaaagtcaa tatctatttc
    47341 agcgacataa actctttgac ctagattgta gttttctata acatctggat gcaattcacc
    47401 taatgtacct acatatttat tattatacac tattttagca catctaccag ggtggaaggt
    47461 agtattatta tcttgaggtt caatttcata acctttaaat ccgacattta caagtatagt
    47521 ttctatagtt ccttttagtg caaagaagtc aacatcttta ccatacatac ctacacacat
    47581 tctattttct tctttaggta attctgaatc ttgtgggatg aatatatgtc cacactcaaa
    47641 tgcagatact tcttctattt tatgagatac attagttgat aaaacatcta acatatttgg
    47701 tattaaagta gttctcatta cagaagtttc ttctcctaat gggttaagta atttaacaaa
    47761 gtttctctta gcattacctt ctggtactct tatcttatcg actcccttag ggcttacaaa
    47821 tgaatatgtt aatatttcat ttaatcctac agcagttgaa ttatctttta aagcatccat
    47881 gaatttttgt tttgaagttt taattcctgc tgtcgtattt ccttctaatt gtacagaagg
    47941 tatattctca aatccataaa ttctagctat ttcttcccat acatcagctt cttgttccat
    48001 atctgttcta aagcttggta catctatttc taatttatca ttagctacca aattacattt
    48061 aaactctaat gattctaaaa ttcctacaaa ctgctccatt ggtacatcta cacctaatag
    48121 gtggttaatt ctttgaggat ttactaccaa ttttttaggt tctggtttat ttggatatac
    48181 atctacaaca ccttttaaaa cttttcctgc tcctaacatt tcaacaagtt gtgcagctct
    48241 atttgctgct atctctgcaa gattagggtc taagtctttt tcatttcttg aagatgcttc
    48301 tgacctaata ccaacttttt tagctgtcat tcttatgttt tctggtttga aattggcact
    48361 ttcaaaaagt acagtcttcg tattagaagt tatttctgag ttagcaccac ccattacacc
    48421 agctaaacca agtgattttt ctccatttgt tataactagc atatctttat ctaatgttct
    48481 ctcaacatca tctaatgtta caagtttctc tccatcttta gcatttctta ctactattct
    48541 tccagtctct acttgattta tatcaaaagc atgaagtggt tgaccaagct ctaacattac
    48601 gaagtttgtt atatcgacta tgttacttat aggtcttact cctgcttctg taagtcttct
    48661 ttgcatccaa tatggagaag gttctatttt tacatctgta accattctag caacatatct
    48721 tctacataaa tctggattat ctatctcaac tttgaaatct atctcttcgt cactttcttt
    48781 tacttcgatt tcaggatatt ttacttttgt tcctatagtt gcagctgctt ctctagctat
    48841 acccatcatg catttacaat caggtctgtt tgaagttaat tcaaaatcta ttaaagcatc
    48901 ttttaatcct aaaacatctt ttatatcttt tcctaattca taagaatctt cgtggtctaa
    48961 aatatatata ccacctcttt tatactcctc aatgtaatgt tcatctatac ctagttcagc
    49021 tgctgaacac atcataccat ctgataattc acctctgaaa tcagtctgtt tgatttcaac
    49081 tcctccaggt aacttagaac catttacagc tactggaata taatctcctt ctgatatatt
    49141 tgtagctcct gtaactattt gaacaacttt atctccaata tctactttag ttacaaccaa
    49201 cttatcagca tttggatgtt gttttatttc taaaatcttt ccaaccaata tattttctat
    49261 ttcttcacca taataatcta ttgtttcaac tttagttcct gtcattgtca ttttatcagc
    49321 gaactctttt acatccatgt ctatatcaac atagtctcta agccatttta aagatactaa
    49381 catctacata ccctcctaat taaaattgat ttaagaatct catatcattt tcgaataata
    49441 atctaatatc atctatttcg tatttaagca ttgcaagtct ttcaacccca actccaaatg
    49501 caaatccact gtaaacttct gggtctattc cacaatttct aagcacattt ggatgaacca
    49561 tacctgaacc taatatttct atccaacctt catatttaca cattgggcaa cctttaccac
    49621 cacatttgaa acaagtaaca tcaacctctg cacttggttc tgtaaatggg aagttgtgag
    49681 gtctaaactt agttttgata tcagaaccaa acaatttttc aacgaagata tccatagttc
    49741 ctttaaattc tgccatagta acatcttttc caacaacaag cccttctatc tgatggaaca
    49801 ttggtgagtg tgtagcatct ggcgagtctg acctaaaaca tctacctggt gcaattactt
    49861 ttattggtaa ctcttgactt ctcatagttc ttacttgaac tggagatgtt tgagttctaa
    49921 gtaatacccc atcattgata tagaatgtat cgctcatatc tcttgatgga tggtctttag
    49981 gagcgtttaa tgcgtcaaag ttgttttcaa cagtctcaac ttctggccct tctgctatag
    50041 agaatcccat tccgataaat atatctgtta cttcatctat aatttgagtt attggatgct
    50101 tcttcccaac ctttaaaact tttcctggtt gagtaacatc tatcacttcc tcttttaatt
    50161 ttctttcttt ttcaatagca tttatttctt cttttttaga atttatgcta agttcaatgt
    50221 tttctcttac ctcattggca accttaccaa ctactggtct ttcttcagca gataatttac
    50281 ccatttcttt aagtatggca gttatctcac ctttttttcc taagtactta actcttaaac
    50341 tttctacact ttctatgctt tgtgcttctt ttatttcagc caaagctgct tcacgtaaag
    50401 caagtaattt ttcttgcaca catatcaccc tttcgttttt ttgtataaaa aaagcccctc
    50461 aatccctgtc tagggacgaa aggctatagt ttcgcggtac caccctagta gtttaacaat
    50521 ttaaaaatca tcaaaccacc tctaacaaac ttaacgcgtt tttgacgtca aagcctactg
    50581 taaaatttca gctctgttac tccagaggga acttctcatg acctcttaga aaacactccc
    50641 agcctaggtg tttatctctt tactaagttt aatcaaaata ctttcctctt tcattgcatt
    50701 tacaatatta tattgtttat tttagtatta tatcatttcc attcaaatta tgtgactatt
    50761 tttaatatat atccaataca taatt
//
LOCUS       DAEPOG010000008        49557 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEPOG010000008 DAEPOG010000000
VERSION     DAEPOG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.008"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            127..582
                     /locus_tag="KR432_000403"
     CDS             127..582
                     /locus_tag="KR432_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0638958.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            631..1215
                     /locus_tag="KR432_000404"
     CDS             631..1215
                     /locus_tag="KR432_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBH0638959.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(1220..1543)
                     /locus_tag="KR432_000405"
     CDS             complement(1220..1543)
                     /locus_tag="KR432_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0638960.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            1695..2213
                     /locus_tag="KR432_000406"
     CDS             1695..2213
                     /locus_tag="KR432_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0638961.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2389..3057)
                     /locus_tag="KR432_000407"
     CDS             complement(2389..3057)
                     /locus_tag="KR432_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638962.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3172..4587
                     /locus_tag="KR432_000408"
     CDS             3172..4587
                     /locus_tag="KR432_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0638963.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4810..5268
                     /locus_tag="KR432_000409"
     CDS             4810..5268
                     /locus_tag="KR432_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0638964.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5265..5849
                     /gene="clpP"
                     /locus_tag="KR432_000410"
     CDS             5265..5849
                     /gene="clpP"
                     /locus_tag="KR432_000410"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0638965.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6171..7247
                     /locus_tag="KR432_000411"
     CDS             6171..7247
                     /locus_tag="KR432_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0638966.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7314..9353
                     /gene="bclA3"
                     /locus_tag="KR432_000412"
     CDS             7314..9353
                     /gene="bclA3"
                     /locus_tag="KR432_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBH0638967.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9522..10283
                     /locus_tag="KR432_000413"
     CDS             9522..10283
                     /locus_tag="KR432_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH0638968.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10592..10870
                     /locus_tag="KR432_000414"
     CDS             10592..10870
                     /locus_tag="KR432_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638969.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            11163..11738
                     /locus_tag="KR432_000415"
     CDS             11163..11738
                     /locus_tag="KR432_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBH0638970.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12175..12885
                     /locus_tag="KR432_000416"
     CDS             12175..12885
                     /locus_tag="KR432_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0638971.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            12879..14195
                     /locus_tag="KR432_000417"
     CDS             12879..14195
                     /locus_tag="KR432_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0638972.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14207..14347
                     /locus_tag="KR432_000418"
     CDS             14207..14347
                     /locus_tag="KR432_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0638973.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14406..14987
                     /locus_tag="KR432_000419"
     CDS             14406..14987
                     /locus_tag="KR432_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0638974.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            15064..16842
                     /locus_tag="KR432_000420"
     CDS             15064..16842
                     /locus_tag="KR432_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638975.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            16857..17729
                     /locus_tag="KR432_000421"
     CDS             16857..17729
                     /locus_tag="KR432_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0638976.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            17730..18518
                     /locus_tag="KR432_000422"
     CDS             17730..18518
                     /locus_tag="KR432_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638977.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(18614..19447)
                     /locus_tag="KR432_000423"
     CDS             complement(18614..19447)
                     /locus_tag="KR432_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBH0638978.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(19515..20144)
                     /locus_tag="KR432_000424"
     CDS             complement(19515..20144)
                     /locus_tag="KR432_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBH0638979.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            20455..21114
                     /gene="cprR"
                     /locus_tag="KR432_000425"
     CDS             20455..21114
                     /gene="cprR"
                     /locus_tag="KR432_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBH0638980.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            21325..22383
                     /locus_tag="KR432_000426"
     CDS             21325..22383
                     /locus_tag="KR432_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0638981.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            22634..24778
                     /gene="fdhF"
                     /locus_tag="KR432_000427"
     CDS             22634..24778
                     /gene="fdhF"
                     /locus_tag="KR432_000427"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:23048..23050,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBH0638982.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            24827..25660
                     /gene="fdhD"
                     /locus_tag="KR432_000428"
     CDS             24827..25660
                     /gene="fdhD"
                     /locus_tag="KR432_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH0638983.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            25671..25844
                     /locus_tag="KR432_000429"
     CDS             25671..25844
                     /locus_tag="KR432_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0638984.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            25902..26453
                     /locus_tag="KR432_000430"
     CDS             25902..26453
                     /locus_tag="KR432_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0638985.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            26478..27854
                     /locus_tag="KR432_000431"
     CDS             26478..27854
                     /locus_tag="KR432_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0638986.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            27854..28369
                     /locus_tag="KR432_000432"
     CDS             27854..28369
                     /locus_tag="KR432_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0638987.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(28447..29730)
                     /locus_tag="KR432_000433"
     CDS             complement(28447..29730)
                     /locus_tag="KR432_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBH0638988.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            29918..30340
                     /locus_tag="KR432_000434"
     CDS             29918..30340
                     /locus_tag="KR432_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBH0638989.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            30442..31380
                     /locus_tag="KR432_000435"
     CDS             30442..31380
                     /locus_tag="KR432_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH0638990.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            31566..33590
                     /gene="ligA"
                     /locus_tag="KR432_000436"
     CDS             31566..33590
                     /gene="ligA"
                     /locus_tag="KR432_000436"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH0638991.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            33750..35099
                     /gene="rph"
                     /locus_tag="KR432_000437"
     CDS             33750..35099
                     /gene="rph"
                     /locus_tag="KR432_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH0638992.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            35099..35569
                     /locus_tag="KR432_000438"
     CDS             35099..35569
                     /locus_tag="KR432_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0638993.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            35763..37049
                     /locus_tag="KR432_000439"
     CDS             35763..37049
                     /locus_tag="KR432_000439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH0638994.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            37198..37782
                     /gene="clpP"
                     /locus_tag="KR432_000440"
     CDS             37198..37782
                     /gene="clpP"
                     /locus_tag="KR432_000440"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0638995.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            37801..39051
                     /gene="clpX"
                     /locus_tag="KR432_000441"
     CDS             37801..39051
                     /gene="clpX"
                     /locus_tag="KR432_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBH0638996.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(39122..39649)
                     /locus_tag="KR432_000442"
     CDS             complement(39122..39649)
                     /locus_tag="KR432_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0638997.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(39637..40149)
                     /locus_tag="KR432_000443"
     CDS             complement(39637..40149)
                     /locus_tag="KR432_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0638998.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            40329..42692
                     /gene="lon"
                     /locus_tag="KR432_000444"
     CDS             40329..42692
                     /gene="lon"
                     /locus_tag="KR432_000444"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH0638999.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            42682..43293
                     /locus_tag="KR432_000445"
     CDS             42682..43293
                     /locus_tag="KR432_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH0639000.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(43558..44928)
                     /locus_tag="KR432_000446"
     CDS             complement(43558..44928)
                     /locus_tag="KR432_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639001.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(45103..45218)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45506..46585
                     /locus_tag="KR432_000447"
     CDS             45506..46585
                     /locus_tag="KR432_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639002.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            46977..47657
                     /locus_tag="KR432_000448"
     CDS             46977..47657
                     /locus_tag="KR432_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0639003.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            47650..49029
                     /locus_tag="KR432_000449"
     CDS             47650..49029
                     /locus_tag="KR432_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0639004.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            49200..>49557
                     /locus_tag="KR432_000450"
     CDS             49200..>49557
                     /locus_tag="KR432_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0639005.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKL"
BASE COUNT        19900 a         5487 c         8722 g        15448 t
ORIGIN      
        1 aagaaaacta aaattttaag gaaaaataac atatttgtat taaattttat tgaaatgatt
       61 aaatttattt acttaatgta aattattagc taaaattgta ttaaataatt aaggaggtga
      121 gtagctctgg atgaaataga tttaaaaata attaattctc taaaaaagaa tagtagggct
      181 tcaacctctg aaataagcag acaaattaat ttatcagtac cagcagtctc agagcgtata
      241 aggaaaattg agcagtcaaa tttaattcaa aagtacacta ttcaaattaa tcgggaaaaa
      301 acaaaatata agcttttggc ttttatattt atcaatactg atttatctaa aagtatgaaa
      361 gaatttaaga aaactatatt ggaatatgat actgtattag aatgtcatca tatttcagga
      421 gagtatgatt acttaataaa agtattagca gaagatacaa gtaatcttga agagtttata
      481 tcagtgactt tgaaggaaat tggggggatt caaaaagcta atacaaccat tgttttatct
      541 acaattaaag aagaatttaa tacaataaaa gatatttttt aaataattta ctagtaaagt
      601 gttaattata ttttaggagg aaaatagaat atgaaagttt tagttttttt agcaaaaggt
      661 tttgaaacta tggaatttag tgtatttgta gatgttatgg gatgggctcg taacgattat
      721 gggcatgata ttgatgtagt gacttgtgga tttaaaaaac aagttatgag tacatttaat
      781 atacaagtat tagttgataa gacgatagaa gaagtatgtg ttgatgatta tgatgcccta
      841 gctattcctg gaggatttga agaatttgga ttttatgatg aagcatatga ttcatctttt
      901 ttaaatctaa taagggagtt taattcaaaa gaaaaaataa tcgcttctat ttgtgtggca
      961 gcattgccag ttggaaaaag tggtgtttta aaaaatcgta aagctactac atatcatttg
     1021 aaaaatggta aaagacaaag acagttaagt gagtttgatg taaatgtagt taatgaacca
     1081 attgttgtag ataaaaatat tataacctca tattgtccag aaacagctcc acatgtagct
     1141 tttaagcttt tagaaatgtt gacatctaga gaacaaatgg atgaagttaa attagcaatg
     1201 ggatttaaac tgtaatgctc tatagacttt ctaagtaatc agaaccccag tcacacatgg
     1261 aatctaaaat tggaataata ctctttccaa aatcagatag cgagtattct acttttggag
     1321 gaacaacagg ataaaccact cttataatta aaccatctga ttctaattca cgtaattgtt
     1381 gagttaacat cttaggcgtt gctttaggaa ttaatttttt taattcatta aacctcaata
     1441 tagtgtcttt aagatgccaa agtataagtg tcttatattt accaccaatc aatgcaagtg
     1501 ttgcttctat tgggcaactg taattatcta aacatttttt catttttgag ctcctttatt
     1561 tattttaaag tatcttttta gatactatat tacaaaaaag tgcattattg taatatagta
     1621 ccatagtgat aaaattatag cataggttaa ataaatcatc aacttagcct aataaaacaa
     1681 tagggggtaa ataaatgaat ttcgttgaac ttacaaaaaa aagatattct tgccgtaatt
     1741 atcaagatag aaaggttgaa aaagaaaaac ttgagaaggt tttagatgtt gcaagaatag
     1801 ctccaactgg tggtaatcgt caacctcaaa gattgattgt tattcaggaa aaagaaggta
     1861 taaataaact ttctaaggct gccaatatat atgatgctcc attagcaatt cttgtatgtg
     1921 gagataaaga caaagtttgg acaagacctt ttgatgggag acaacttaca gatattgaca
     1981 caagtatagt tacagaccat atgatgttac aggcaactga acttggattg gcaagtgttt
     2041 gggtgtgcta ttttaatcca gatataatta gagaagagtt tagtttgcca gataatctag
     2101 aaccaataaa tatattattg atgggatatg aatctaagat accagagagc ccagagagac
     2161 atgaaaaaac tcgtgttcct ttaagtgaaa ttgtttctta tgaaactctt taattataaa
     2221 attaagttat tcataggata aatgtaataa agtgtttatt ataaacaaaa ttcaaaataa
     2281 ataatatcta catataatta attttagtga agtatatcta gtttaactag aatgtatact
     2341 tcactaattt atttattcaa aatatttatg aggattttaa tatattaact aaaatatatc
     2401 aattgaatta acttttaaat ctggaaaagc acttgaaata tagtgtttta tgtcatttaa
     2461 atggtcttga atttgctttt ctgatatttt atcaggtcca tatacaaaat acataatatt
     2521 tttagtatct tttgttatag ctgttcgatt atcagggcca ttaagaatac tagataatat
     2581 accaatatta tctgataaaa ataaatcatc ttttgttaaa atatgctctt taccacctat
     2641 accataaaaa tgttcacttc catttgctaa ttctattttc aaaggtaatt ccattttatc
     2701 aaggtcatga cctgctgtta gaagaagatt tttcaattct gctaaaaaca ttgactcaat
     2761 tgttacacct acatctggga taccttttga ttttaataga atagattcta attgaagcaa
     2821 tacagggtaa gttttcttga attttttata atactttata taacttgaaa caggctctgt
     2881 tttaattttt tctttacgat tgtattctgg gtgttgttct ataatatttt taattgtaga
     2941 gtttttaaga actaaaaaat tttctttatt aggttttgaa tgtgtaatat ttataaccat
     3001 aactccaaat ttaatattgg gataaatttc tttaacttta tgtgatattt ctaacataat
     3061 atagactcct ttttatttta tattttatga tataatctta gcaagttaaa agtggatttt
     3121 gtagttccat ttttatagta attttataga accacttttg gaggtattag aatgtggatt
     3181 acgattaata gaaatagtaa tatatcatta tcaagacaaa tttattgtga gataaaagat
     3241 atgattctgg atggaagctt aaagtttgag caaaaactgc catcatctag gactttatca
     3301 aaagaactat ctgtatctcg taatacagtt ttagatgcat ataatcaatt aattgcagaa
     3361 ggatatttgg aaacaaggaa tggatatgga acaattgtag ctaaaggaat atcagggcat
     3421 aaagttgatt ttaagttaaa tgatactttt agagatgaaa aagatttaca tactaataaa
     3481 caaaaaaatt atatagattt tcgttcagga gttccagaat taaaactatt tccacgcaaa
     3541 gaactttcaa agctatataa acaaatactg tatgatttat cagattcgga tttcagatat
     3601 ggaagtacag ctggtgtatg ggttttaaga gtggaaatat ctaagtattt atttcgtact
     3661 agaggaatta gatgtaattc caaaaacatt atgattgttt ctggttcaac acaaggttta
     3721 tctttaattt cacagctttt atacaaaagt aataaaaagg ttgctgtaga agaccctatt
     3781 cataaaggct tattaaaggt tatttcatct attggatatt cagttgttgg aattaaagta
     3841 gatgataagg gaatgaatac caacttaata aaatcaataa aggggatttc ttttgtttat
     3901 actacacctt ctcatcaata tccattagga gggattttac ctattcaaag aagactagag
     3961 cttattaaat gtgcaacaga gaatgattac tatataattg aagatgatta tgatagtgaa
     4021 tttagatatg agggtcaacc aattagttct ctttatgaac ttagcccaaa taaagtaatt
     4081 tacataggtt cttttagtaa aatattatct ccagctattc gtgttggatt tatgttatta
     4141 ccagacaata ttttagaaag ctataaaacc ttaaaaatgt actctgatgt tcatacagaa
     4201 acaatattgc aatatgtatt agctgaattt attggaaatg gaggtttaga gaagtacata
     4261 tttagaatga agaagttgta caatagaaaa cgtaaatatc tcataaaagt tttaaacacg
     4321 gaatttaaaa atgaatttaa aataaaaggt gaagcagcag ggcttcatat agttgctcat
     4381 ttctataatg ttaaatttac ggaaagttta attcataaaa tttttgaagc aggtataaaa
     4441 gtttatccta tacaacaata tgcaattgag aatatattag aatatgacca tgaaattata
     4501 ctaggttatg ctcatcttag ttttgatgaa attgaaaaag gtgtaaaaat actcagcaaa
     4561 ataatacata tgaatcaagg tacatagata aaacatgtag caatctacat ataatagatg
     4621 taattaagtt taaactttaa aagaaatata tattaatatt tttgtataaa aatacttgat
     4681 attgttttat cgatacttta ttgctataca ctaaattaag atagcaattt atattaagaa
     4741 aaaacttttt gcataggtta taatacttaa aaggagagga tagagttgaa aacaaataca
     4801 gatgttatta tggagataat tgattatata gaagaaaatc ttacagaaaa tctaaattta
     4861 gataaaatag cttatattgc taattattca aaataccatt tacacagaat gtttacaaat
     4921 gttgtaggat gtactatcca tcaatacata cagaggagga gacttactga agcagctaga
     4981 caacttatat ttacagacaa atatattata gatatagcta ttacagtagg atattctaca
     5041 catcaatctt ttacactagc atttagaaat ttatataaag aatctccaca agtatataga
     5101 aataaagatg aattttatcc tattcaacag agatttgttt taaatctaaa tcatcaaatt
     5161 caaagcgaaa aatgtatttc ttatgcaaat atgaattata aacaaagaag agatgaaatc
     5221 aattcttatc ttaactttaa tattaattgt gaggtgaaag tagcatgagt ttagtgccac
     5281 atgtaattga acaaacaggt caaggggaaa ggtcttatga tatatattca aaattattaa
     5341 aagatagaat tatatttata ggagaagaaa ttaatgatgc tattgcaagt ttagtagtag
     5401 ctcaattact gtttttagaa tcagaagacc cagattcaga tataattata tatataaata
     5461 gccctggtgg ctctgctaca tcaggatttg ctatatatga tactatgaat tatgtcagat
     5521 gtgatgtatc gactatctgt attggtatgg ctgcaagtat gagtgctttt ttacttgcag
     5581 gaggaacaca tggaaaaaga tgtgctttac ccaattcaga gattatgatt catcagccaa
     5641 tgggaggagc taaaggtcaa gcaacagatg taaaaatagc tgtagataat atattgaaaa
     5701 taaaagaacg attagataaa attctttctg aaaatacagg taaatcaata gaagatatta
     5761 gaagagatac agatagagat aattttatga ctgcactaga ggctaaagag tacgggctga
     5821 ttgattatat aatgaatagg aatgaatagg aatgaataaa gtatcaaagt atcatattaa
     5881 aatgtctgtt ttacaaatat atatataata ttaaataaga gtgtctcaaa atgaactttt
     5941 tagtttatgg gacactcttt tttgtatgaa attaaatatg gttttatcat ttcaacaatt
     6001 attaccaaat ttcaagttta ataaattcta ctataaaaaa tacataaaag ataaggttct
     6061 ttagtaactt tctgtcgtca aaagttttta taaatttaat aacaaaacaa gagaaaatac
     6121 catatattac ttatgtctgc aatatataga agacaaagga gttgtacatt atgattgcaa
     6181 taagtttgtg catgattgtt aagaatgagg aagaagttat aggcagatgc ttagaatgtg
     6241 taaaggatat agtagacgaa attataattg tagataccgg ttctacagat tccacaaaag
     6301 aaatagtctc aaaatataca gacatggtgt acgatttcga atggatagat gatttctctg
     6361 ctgcaagaaa tttttcattt tctaaagctt ctaaagacta tactatgtgg cttgatgcag
     6421 atgacataat tttagaagct gatagagaaa aattattaaa gattaaagaa agcttggata
     6481 cttccattga tattgttatg gcaaaataca atgtatcctt tgatgaaaat ggtaacccaa
     6541 ctctctcata ttatagagaa cgattgttta aaagaagttc taatcataaa tgggttgacc
     6601 ctattcatga agtagtacct ctttttggag aggtatttta ttctgacatt gcaatatctc
     6661 ataaaaagct tcatagacag gaccctctaa gaaatcttag aatttttgaa aaaatgattt
     6721 cagaaggaaa aacattggag ccaagacacc aattctatta ctctagagag ttgtactata
     6781 atgctaggta taaagaagca atagaaggtt ttactaagtt tttagactca tcaagaggat
     6841 ggattgagga ttgtatcagt gcctgcagag atttagccac atgttattac ttaattaatg
     6901 atgaaaaaag tgccttatat tcattgttta gaagtttcga atttgatgag ccaagagcag
     6961 aaatttgttg tgacatagga aagcatatgt ttgatagaca aaaatataaa gaagccattt
     7021 tttggtataa ggtagcatta acaagagata agaatgatac taatggaggt tttaagtcaa
     7081 atgattgtta tggatatata ccatctatac aattaagtgt ctgttatgac aggttaggag
     7141 aatcagataa agctatttat tatcatgaaa aaacaaaaga aatcaaacct aacgatagtg
     7201 ctgttttgca caatgaaaat tattttagta aatttaaaaa tagttgatag gtcacaatca
     7261 tactaaattt gacatattat atatagagct taaaagctca aatatatcag gaggtgcttt
     7321 taataatgag tagaaataaa tattttggac catttgatga taatgattac aacaatggct
     7381 atgataaata tgatgattgt aataatggtc gtgatgatta taatagctgt gattgccatc
     7441 attgctgtcc accatcatgt gtaggtccaa caggcccaat gggtccaaga ggtagaaccg
     7501 gcccaacagg accaacgggt ccaacaggtc caggagtagg gggaacaggc ccaacaggac
     7561 caaccggtcc gactggccca acaggaaata cagggaatac aggagcaaca ggattaagag
     7621 gtccaacagg agcaacaggg ggaacaggcc caacaggagc gacaggagct atagggtttg
     7681 gagtaacagg cccaacaggc ccaacaggag cgacaggagc aacaggagca gatggagtaa
     7741 caggtccaac aggtccaacg ggagcaacag gagcagatgg aataacaggt ccaacaggag
     7801 caacaggggc aacaggattt ggagtaacag gtccaacagg cccaacagga gcaacaggag
     7861 taggagtaac aggagcaaca ggattaatag gtccaacagg agcgacagga acacctggag
     7921 caacaggtcc aacaggggca ataggagcaa caggaatagg aataacaggt ccaacaggag
     7981 caacaggagc aacaggggca gatggagcaa caggagtaac aggcccaaca ggcccaacag
     8041 gggcaacagg agcagatgga gtaacaggcc caacaggagc aacaggagca acaggaatag
     8101 gaataacagg cccaacaggg gcaacaggag caacaggaat aggaataaca ggagcaacag
     8161 ggttaatagg tccaaccgga gcaaccggag caaccggagc aacaggccca acaggagtaa
     8221 caggggcaac aggagcagca ggactaatag gaccaaccgg ggcaacagga gtaaccggag
     8281 cagatggagc aacaggagcg acaggggcaa ccggagcaac aggtccaaca ggagcagatg
     8341 gattagtagg tccaacagga gcaacagggg caacaggagc agatggatta gtaggcccaa
     8401 caggtccaac aggggcaacc ggagtaggaa taactggagc aaccggagca acaggagcga
     8461 caggtccaac aggagcagat ggattagtag gtccaaccgg agcgacggga gcaacaggag
     8521 cagatggagt agcaggtcca accggagcaa caggggcaac aggaaataca ggagcagatg
     8581 gagcaacagg tccaacaggg gcaacaggtc caacaggagc agacggatta gtaggtccaa
     8641 caggagcaac cggagcaaca ggattagcag gagcaaccgg agcaacaggc ccaataggag
     8701 caacaggtcc aacaggagca gatggagcaa caggggcaac cggagcaaca ggtccaacag
     8761 gggcagatgg attagtaggt ccaaccggag caacgggagc aacaggggca acaggtccaa
     8821 caggcccaac aggtgctagt gcaataatac cttttgcatc aggtatacca ctatcactta
     8881 caactatagc tggaggatta gtaggtacac caggttttgt tggctttggt agttcagctc
     8941 caggattaag tatagttggt ggagtaatag accttacaaa cgcagcaggg acattgacta
     9001 actttgcatt ttcaatgcca agagatggaa caataacatc tatttcagca tacttcagta
     9061 caacagcagc actttcactt gttggttcaa caattacaat tacagcaaca ctttaccaat
     9121 ctactgcacc aaataactca tttacagctg taccaggagc gacagttaca ctagctccac
     9181 cacttacagg tatattatca gttggttcaa tttctagtgg aattgtaaca ggattaaata
     9241 tagcagcaac agcagaaact cgattcttac tagtatttac tgcaacagct tcaggtcttt
     9301 cattagttaa tactgtagca ggatatgcaa gtgcaggaat tgcaataaat tagattagat
     9361 aagaaaaggc ttctaatatc gtattgatat taggagcttt tttaagaatg attgatgaac
     9421 atagttttaa tattaattag tgcgcatatt ataaaatact attatataat ataaaataat
     9481 aacattaaat tggaaatata gaattatcta ggggtgatac tatgaaaaat aagattgaaa
     9541 catcaatgaa caactttgta aaaaattatc ataaatcaaa taatataact acaaagtggc
     9601 aagaactttt gtttgcttat gcatctgctg aagatgatat gtttcagaag ctaaaaagtg
     9661 ttgtaggaac atctcatgct atgccaaaag attttctgga aaatggaaaa actataatta
     9721 catattttat accttttgat gaaagtgtaa ataatagtaa tattggaggg tttgaatgtt
     9781 ctgaaatatg ggcaagagcg tacatagaaa ctaatatgtt gatttcaaat ttaaatgatt
     9841 tcttatgtga agaactagag aagatggggg ctaattcata tgctattcct gggtcgtata
     9901 attttgacag tgaaaaattg gtaagtgact ggtcacaaag acatgtagca ttcatagcag
     9961 gtcttggaac atttggttta aataatatgc ttattacaga aaaagggtgt tgtggaagaa
    10021 taggaagcat aatcacaagt ttagaattag agccaactaa aagaccagaa aatgaatact
    10081 gcttgtataa acataataat agttgcaaaa aatgtgtcaa caaatgcgtg aaagaagctt
    10141 taaaagaaga tggatttaat aggaataagt gttatgaaat gtgtatgata aatgaaaaaa
    10201 aatacactta tataggtggt tgtgatattt gtggaaagtg tatagttggg gttccttgtt
    10261 cggtaataaa tccctcaaga taaaggactt atgttgatta ataactttaa atatatctaa
    10321 taagaagaaa aaagttagtc tctaaaatag atgaattatg aaaataaata aagataatat
    10381 aaaggttaga tgaagaagtt atgttaattg acaactattt atactgctga tagacttata
    10441 tgtatcgttg caatatataa ggaggggtga gatttatgtt tgatttaatc tttatttttg
    10501 catagcaagg gctattgcaa atataaaatt taatatataa atagcccttt gctattccta
    10561 ataaaaaatt attatttaat aggagtgcag aatgagctat aaaaaagcaa tacatatatt
    10621 accagaagaa ttacttgaat tgatacagga atatgtagat ggagaatata tctatattcc
    10681 tagaaagtct aataataaaa aagaatgggg aagtagtacg tctactcgtg aagatttatc
    10741 aattagaaat atgcaaattt atgaagatta tcaagttgga tatgatttga aatatttatc
    10801 agaaaaatat tacttgtctt tgaaaagtat tcaaagaatt gtattacaag aaaaaagaaa
    10861 agatagttaa gaatgagcca gtatagtttt ataattatac tggctcattt tcttttgata
    10921 aaagtgtttg aggtatagtt tcttataagt ggatgctata atttaaataa tttcaataac
    10981 ttgtacagca gataacgaat agagttgtaa agtatctaat aagaagtagt agctttttat
    11041 atgtttgaaa ttaaaattta ttaaaattag gaggtcttgt tttgaaaaaa atacttgatt
    11101 atattccacc ttttcataac tttctattaa tttagtaatc ttatagaaag gaagttgcat
    11161 ttatgaaaaa aattacttgg gaggtggaat atatctccca gttacctgta attaggtatt
    11221 gtaagaaatg tggaaaaaag actgaatata tttgttcagg tttatttagg gttaatgctc
    11281 aacataaata tttggatata tggttaatat acaagtgttc aaattgtgat tcaacatgga
    11341 actcaactat atattctcgt gtcaatccaa aaagcttaaa tccagaaaca ctagaacaat
    11401 tccatacaaa cgattataat cttgctaagc aatatgctat gaatacggaa cttttacata
    11461 gaaatggagc agaagttggt attccagagt ataaaatagt aggacaagaa attaatatta
    11521 atgaccttac acaagttcat attattagta aatatccatg tcagttaaag gtatcaacat
    11581 tattacgtga gaaactaggt ttatctagga aggtatttga cgaaatgttg gattgtggga
    11641 ttattagaag taactcagga gttgatttaa agaaaggtaa attaaattat gaaattatgt
    11701 tagatatagg caaggagaag caaaagtctt caaattgatt agaataacta acttaatata
    11761 ttttcaggaa catcatttaa aaaaatagtg gcttaaaaaa tttaaagcca ctatttttta
    11821 atatgtttta tatgaatatt taaaatatag tgtgtattct tcaaaattat agcgataatt
    11881 aaaatattac tataaaaagg aaggtgcata ctttttgttt aacaaaaact agatactaaa
    11941 aacttctcga agaatatgtt attaaaatcg ctaaatatat tcaatatata ttaaaaaatt
    12001 gtattataat gaaaattaaa atagattgta aatccaaaaa gtaatactta atgtccaaaa
    12061 agctaaaatt aaaatctaaa tagctatttg ctacagaagt atgttaaaat atgtaaaatg
    12121 aatagaaatt ttatatattt attaaaaata tataagaaag gatgtttaag aattgtgatt
    12181 agtataggaa tatgtgatga tgaactacat tatcgaataa aaataaaaga tatattaagt
    12241 gaaatattaa gttcatatcc tataaattat aacatatatg aattttcatc aggagaagag
    12301 ttactaaata actatcctaa agatttagac atattaatta tggacataca aatgaaaaca
    12361 ataaatggta tggatactgc taggaagata agagagtttg atcataagtt agaaataata
    12421 tttgttactt catttgtaga gtttatgcaa gaaggttatg aggtaaaagc atacagatat
    12481 atactaaagc caataaataa agaaaaaatt tctaaaagtg tattaccttg tataaatgag
    12541 atgatgaaaa agagaaataa ttacttaact ataaatgtaa aaaattatgt agaccgaatt
    12601 aaaatagatt ctattactta tatagaaaca gatagaccga atatattgat ttatacacat
    12661 gatgatatgt atattactaa aatgagtata tctaaaatag aaaagatatt aaatgaatat
    12721 ggatttttta gatgtcataa tagctatata gttaatttaa aattagtaga atcaatgagt
    12781 ggaagtactg ttatagtaga tggcaaaagt attccaataa gtaaatatag agttaaaggc
    12841 ttgaaacttg ctataactaa tatattaggg gatatagtat gctaacaaat ctaagtatag
    12901 gattttggaa tattattaat ttagtttcag gtactataga ttggattata ttttttttag
    12961 taatccactt agttggaaag aggaaaatat ctaatataag atatatattt acaacaataa
    13021 ttatatttat atttatggca tacataaatg tagttaaagt gtttccaaat attaagattc
    13081 ttctatgtat ggtaatagga atatcatttt atataacatc atatgaagat aaaatatata
    13141 aaagtataat agttagttta ttgttttggc taggattaat gattgctgaa ggttctgcag
    13201 taggtctagt agtatttata aataaattaa ataatattaa cataacacaa catgaaaact
    13261 tatttagaat acaagctatt attatgtcaa aggtattttt atttataggt tttatattgt
    13321 ttaagtattt taaattatct ttagatttta aacctaaaga tatgatacta ataggaatac
    13381 ctatattatc taatatagtt agcttattac ttatatttga atataattta agagtcaata
    13441 caattactac agaaaactta tttatattta tatttgcaat tttattgatt ttatcatcaa
    13501 gtataatact tttaatagta atagggaaaa ttgtacaaga cgataaatta aatctagagt
    13561 atgaacttat aaatgaaaga ataaaaacaa actacaggaa ttatgaaaat ataaacgaag
    13621 ttcatgatag attaaaatat gtttatcatg atttgaaaaa ccatatgatt tgtataaaaa
    13681 attatgacac taaagaagaa attatatcat atatagataa cttagagttt cagataagtg
    13741 aatttgaaca ttttaaaaat actggaaata aaacattaga tttaatttta gcagaaaaaa
    13801 taagtatttg taaaaagtat gatattcaaa ttgaagataa cataaatata tctaaattaa
    13861 actttattga aaataatgat atatgttcaa tatttgcaaa ttcactagat aatgcaattg
    13921 aagcatgtat ggatataaat aatgagctag aaaaaagaat agaagttaaa gctacatata
    13981 taaataaatt tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg
    14041 ataaacgtat tcaaactagt aaggatgatg ataagataca tggaatagga cttgctagta
    14101 ttaaatatat agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt
    14161 ttatattaaa aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc
    14221 gttaaactta cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa
    14281 cagtgcaagt tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa
    14341 aaaataacat taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag
    14401 gaaatatgtt taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg
    14461 tagaaagtga agattttgaa atatatagat atggatttga aactttgatt tatttcatag
    14521 ttaatatttc ggtagcatta tttataggaa ttatttttga tagatttata catacagtta
    14581 tatttttaag ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt
    14641 ataaagagtg tacattaaca tttgcagtta tttatttgat aactattttt tcagctaata
    14701 atatagatat aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa
    14761 caatttacaa gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa
    14821 agcattatag aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat
    14881 gtaaaatact taatattttt cagcaatatg tgatttatgc aataatttct atatattgga
    14941 ttgctatatt aatatatatt ggaatgaagg ttaataatga tcagtaagat tataaaacat
    15001 tttctaaatt aattaatttc actaattaat attaatttta aaaaataatg tcaaggtaaa
    15061 tatttgaact tattaaatat agttgaatct attgtaagtt gtatcaaagg attggctagg
    15121 aggggcataa atatgagtag tattaaggtt atatcagact ttataaccaa aataattaga
    15181 aaaaatattt tatttgcaat aacagttatt attataaatt accatctcat acaacgatgg
    15241 acacatatat ccaatagagg aatttcattt aatgattact tatccttgag tgtatatact
    15301 tgtatgacaa ttagtatttt aaccatgtta aatgtaattt tttatatgag aaaaagttat
    15361 gatactgtaa aaatattatg tagaaataat tttgattatt ttttaggtat ttctgttgct
    15421 gtaactagaa acaactttat attttgttgc ataccaatta tatatgctat agcatactca
    15481 agtttaataa gaacattatc aattgtaacg attaaatcaa ctatatttat attggtattt
    15541 gaatggtttt taccacttac tattttatct atagtgatat catttatttc agttattata
    15601 aataatttaa tactaaagat taccttatca tcagtatttt tatatttaac atcaactaag
    15661 atgttggata gagttattga tattaaagaa ccatttttta aagcattatt taaatcactt
    15721 aatatatttg aagatcaatc atatgcttac tattctgatt ttgtagggaa agttaataat
    15781 tcagcatatg ttatagataa gattatacct acactatttg caattacttt aataatagta
    15841 gcatatctga tattaaatga tattagaaag aaacagatta aatcattaat agttatagta
    15901 gtgtacagta ttttatttat tggattaaat tatatatcaa tagatatgtc aacatataga
    15961 gatgaggaac gtagttatgg agagtattta aagaaatcta atattagtga tattaaaata
    16021 aaatcgcatg atatgaagat tgactttaaa agcaagtcaa atataaaaga tagaattgtt
    16081 ttattaaaca catcaaatga agagttaaca aatattaata tgattttaga tgaaatattt
    16141 gatataaaat atataaaagt aaattcggaa aatataaaat ttaacaaaaa aaaggattta
    16201 attgaagtta atttaaagga aagtttagag cctaatgacg aggtgacatt agatatagag
    16261 tatgaaggct atattaatgt gttgacatct tggaatcaaa ataaatatat agctactaaa
    16321 gatgatatta tgcttccacc aggttcttta gcgtggtacc caaaagtaaa tcaatcaagt
    16381 tgtatggctt ataaaataaa tttaagttct aaaaataatg tctattcaaa tttaccttta
    16441 ctgaataaga ctagtaattt tttaaaaaaa gattatatat ttgaaggaaa gtcaaatgat
    16501 attgcaatat atgcaggtga gtttaaagaa gaggtaaaag atgatataaa aataatctat
    16561 ccatcagata gtaatgtaaa tatggatata gaggaaaaaa ttaaattttt aattgaaaca
    16621 agaaatgaaa tattaggtga tagcaaagca tctgtagatg ctaaaaaata tgtagatgaa
    16681 gtagataatg ttatggaatc aataaaaaga aaaaaaatta atatgatatt agttgctgac
    16741 ttacctagca atgaattttt atatgaagac tcaaagggag agaaaattag cataactatt
    16801 tatcattggc tttcaggaaa tatccttata atagataaat aataaaggag gttaatatga
    16861 taaacaagtt agaaataaga aatctaaatt ttaattacaa aaataaaaaa gctctggata
    16921 atataaattt aaccctaaca gatggagtag ttgcattatt aggtcctaat ggtgctggaa
    16981 aatcaacatt aatgaggtta ttagtgactc tttatgaaac ttcaattgga gagatagaat
    17041 taaataatat aaagtatagc aaaaacaatg agaaaatcaa ggcaaatgta ggatatatac
    17101 ctcaagattt tgatatgtat aataatataa atgggcaaga atacttagag tttgtagcta
    17161 aaatgagagg tgtatctaag aatgatttaa agaaacatat acaaaaagta gtatcaaaag
    17221 ttaatctgga caagtttata aataataaaa taggtacata ctcaggtggt gtaaagagac
    17281 gactaggtat agcacaggct ttaataggtg attctaagtt aatagttatg gatgaaccta
    17341 cagttggtct tgatccagag caaaggaatg aatttagaag attgttacca gaaataagta
    17401 aaaattctat agtattaatt tcaactcaca tagtagaaga catacaattt aactgtgaca
    17461 aattaattat attaaatcaa ggcaaaatat tatatgatgg tacaattaat aaatttattg
    17521 atatggttga ggtgtattca gtagtagttt ctaatgatga atttgaatat ttagaacgta
    17581 atattgatat tattgattat agaagagtta aagatggtgt gcaagtaaag tatataggta
    17641 aaaatacaga gaaacttaag gatagcttaa ggatagatac aactttacaa gatgcttata
    17701 taaacttttt acataaggag ttaaaataaa tgtgtatatt ggagttaata aaatttgagc
    17761 ttaaaaaaat agagcttaaa catgtgttaa tatttgtaat tatttctata ttatgcatat
    17821 ttattacagg catattagat tataatgagt taatatttta taatgatgta cctataatgg
    17881 atacatattc tatagcagga tggttaggaa ttataaagat atcagatgtt tttataccaa
    17941 taatgtcagc aatcacaata atttatgtgt tttcaaaaga ttatttgggt aatataaatg
    18001 agatacttac tttatataat aaaaaaaaat ataatcaatt tatagtattt agatggtgtg
    18061 ttatattagc tatatatact gtattattat taatttcaat tacaatagta tatacattta
    18121 cgtcagtgaa aggttttaac atagacaaaa tagattttgt ttatagtgta aagccaatag
    18181 atgtgttttt aaagacattt cctacattat tatggtatac aacatttcca ctaatgatat
    18241 taacattaac aaaaaataga tttacaacaa tagcaatatt aattacatat acatttgctg
    18301 atatattttt tattttacat atgtacccat ttgttagtat gattaatgta aatggatttt
    18361 atattcaaaa gatgttttat atgcctaata atatagatgt tagatttaat gaattaaaga
    18421 attatttctt tataaataga ggttctctag tagttatttc tataataata gttttttata
    18481 tagcaagaaa atcaacggtt ttaaaaaaga gtaaatagtt taatatattt tttataatgt
    18541 tatatttata tagaatagtg actttaaaaa atttaaagcc actatttttt aatatgtttt
    18601 atctatataa ctattatttt tctaggtttt ttaatatttc gtaatagtgt gttgtggcta
    18661 tttcacttga ctcgcctttt ttacaaaaag ataatttaga tagttttgga actacacgtt
    18721 caacgacaat tttattacct atcattttag attcaagtat cttcaatgct tctaaaaatc
    18781 tcttatcctt ttttgcacga ttataaaatg ataaaacgta gacataaagg aataggttat
    18841 aattacggaa aggatattca acttgcataa ataatgtgcc aattccatag tgacaagggc
    18901 cgagtggttt tttaatagtc caatggtcaa gtagaaaatc tacagctttg tcaagttttg
    18961 attctttatt tagataatta ctaaaacgga atgcatttaa tatattaaga gttggtagtg
    19021 gatttgagta ttcagtttct ggacctcgac caaaactaaa tttattgcaa cgccacccac
    19081 catcagtata ctgtgtatct aaaaaatgtt gaaacgtttt ttgcattcta atgtcagatg
    19141 catatcccat atgacagagt acgttagcag catttgcagt atgacaaggg taaatacctc
    19201 catttggata tagtttgaaa cgtccatctt ctcgccatgt gctaaatatc aagtcagcac
    19261 attctttaag taaagtatca tcagggttca cacctaactc taatagataa agtacactat
    19321 caagagtaga gaaaggagaa ccttttatta aacgcttatc aggtgtagtc caatagtctg
    19381 caccattatc atacctgtgt gataatatta tttcaatatc tgataaagat tgattttcta
    19441 cattcatttt ttaccccatt tcatattata ttctattaaa tctatcaaaa actttattat
    19501 aatattattt atatttataa ttgatttatt ttatttaaaa ttgcatttat agctgtatca
    19561 ggaacaactg ctaaatcttc tgcattgaag taataaacta tattagaatc aaataatata
    19621 tttgaatttg gaggaaattc atcatctcca aaccaaagta taaatatcat agatgtatta
    19681 cccatatatg taaacctata agctaaatct cccatgttta ctttttcagc tccaattttc
    19741 tccataacta acttatattt gtctatttga gatgtaaaaa cttctgaaag cctatataga
    19801 gtcctgttta aaaaatttgg ataataaaca ttgcctcctg gtatttcttt aaaagttaca
    19861 tatttattag ttctagggat acctttagca ttgattaaat atctaactat caagatttta
    19921 agctcaaagt tattgcaaag gctaccatta ctattccaaa cttctccact tggatagtct
    19981 ataatatatt gtctatcaaa aaaattgagt gtgaatctat ttaaatcatt attgaaaggt
    20041 atattactta aattactaat tgtatgaggg tctaattctt tgagtttttt tcgaacatag
    20101 tcaaatggaa cattataatt attttttgtt atattttcat ccataatttt ttcctccaat
    20161 ttaaacaatt aaaagtgtat ttataaaaag ttttcttatt attaatttta ttatatattc
    20221 aaaatattac aataataaat tgttaaataa atttttatat ttaagtaaat gcgtgtaaaa
    20281 tgaatataaa gtttttgaaa tataaagttt ttgtatctaa aatattttat ttgtaaaata
    20341 gtaattaatt attgtaagct ataatatgag agtattatgg ttaatgtgaa ttttaaagat
    20401 attattaaag atataaatta gaatactatt aaacggataa atggaggagt tattatgtct
    20461 aagatattag tagtagatga tgagttagat atgttaaagt tgattgaaaa tgtgttgaaa
    20521 agagatggac atgaagtaaa ggtaattagt gatgtagaaa aggctatgaa catggatttt
    20581 aattatttta atctaataat attagatgtt atgatgccta agatggatgg ttttgaagta
    20641 tgtaaaacta taagaaataa ggttgattgt ccaatactgt ttttgacagc aaaaaatatg
    20701 gaaagtgata ttatgtacgg tcttggcata ggtgcagatg actatataac caaacctttt
    20761 ggtgtaggag aattaagggc taggataact gctcatttaa ggcgagaaag tagagaaaaa
    20821 aagaatttat taactatctc aaatgttcaa tttaactttt taggcaaaga agtttcaatt
    20881 gatggtaaaa aaattaattt tactaaaagt gaatatctaa tatgtgaatt tctagcaaaa
    20941 aataagggac aagtattttc aaaagaaaga atatatgagt cggtatatgg ttttgatgga
    21001 gaaagtgata taagtactat cacagagcat ataaaaaata tacgaagcaa attaaaacaa
    21061 tatgatattt atacaataga aactgtatgg gggatagggt ataaatgggt gtaataagta
    21121 taagtgttgc ttagtgaaaa tattatatat atctacaaaa tgtttgtaaa atattaatct
    21181 atatattaaa atttaattta tagattaata ttttttgtaa atgacaaaat tgtaaggtat
    21241 tttgttataa aatattaaga ttcatagtgt atactaataa tatagtattt agtataaatc
    21301 ttgagcaaag gatggttatt tctaatggat agactaaaga atattagaaa gagtttacta
    21361 cagtatacat ttttattttt gttaataggt ataacaattt acctagtgtt taaaacattg
    21421 gatattaaaa tgctatcaaa tgttatggtt atggtagata agaagttttt attcataggt
    21481 gctttggcaa taatgtgtca cataatgctt gaaggagtag taatgaagat aattatagaa
    21541 agcactcaca aagttaatat tagatttatt ggttttaagc tagccattat gggattttat
    21601 tataatttaa taactccttt tgcttcagga agccaaccag tacagatata tgtacttaaa
    21661 aaatgtaaaa tgccacttag taaggcaagt gcagttgtta ccaataagtc tattattttc
    21721 caaatagtgg ttacttttta ttgtacaata cttgtattaa tgaattttgc tatgttagaa
    21781 aaacaaatga aaatgattat gcctctagtg tttctaggga tagccattaa ttcatttgtc
    21841 ttaattatga taatattagt tattttaaac cctaaaaaaa taaaatattt tatgagaatt
    21901 actataaagc atttatcaaa aattaagttt ttaaaatttt tagataataa gattgaatcc
    21961 atagaaagtt ttatagatga ttatagtaaa gcaataaatt tttttgcaaa gaataaaaag
    22021 gtattaattt caactataat agtaactttt attcaattaa gtgcttactt tagtgtttca
    22081 ttttggatat ataaagcatt taatttacaa ggttatagct atatttatat actgacatta
    22141 caagcatttt tatatatggc aatatctcca ataccaacac ctgggaatgt aggagctaat
    22201 gaattagcct ttttcactat attcaaatca gtattccctc aacctttgat gggatatgca
    22261 gtatttttat atggtggatt tatgtactat ttaattctga ttggaagtgg aatatttact
    22321 gtaattactc actatagaat gaaaaataga attggcagaa atattgtttt aagtgaaaac
    22381 taaataagtt taaagaatat atattgagta tattaaaaaa tatgtcataa gaactagaat
    22441 attttaatta aatggttgaa atataaagat tattatgaca tatttttttt gaaatttaag
    22501 aggaattgtg agaaaattgt tgaatttaat agatagtatt gcaaaatatt tatgattttg
    22561 catattataa aaacactcat tatctttaag ataaatttta cttatcaggg gataaatcta
    22621 aggggggaat tgtatggaga aaaaaatttt aacagtttgt ccatactgtg gagctggatg
    22681 ccaattatat ctagtagtaa aagataataa gatattaaga gctgaaccgg ctaatggtag
    22741 aacaaatcaa gaaagtttgt gtttaaaagg tcgttatgga tgggattttc taaatgaccc
    22801 taaaattcta acacctaggc taaaaaagcc aatgataaga aaacaaggtg tactagaaga
    22861 ggttgaatgg gatgaggcta taaactatac agcttcaaga ctaaatgaaa ttaaagaaaa
    22921 atatggacct gattctatta tgggaacagg ttctgcaaga ggacctggta atgaagcaaa
    22981 ctatataatg caaaaattta tgagagctac aataggaact aataatgttg accattgtgc
    23041 tcgtgtatga catgcaccat ctgtagccgg tctggcttac tcattaggta gtggtgcaat
    23101 gtctaattct ataccagaga tagaaaatgc agatgtttta tttatatttg gatataatgg
    23161 agcagattca catccaatag tggcaaatag aatagtaaaa gctaaaaaaa atggtgctaa
    23221 attaattgtt actgacccta gagtaactga atctgcaaga attgctgata tacatctacc
    23281 tataaaaggt ggaacaaaca tggttttagt aaatgctttt ggaaatgtat taatagaaga
    23341 aggtctttac aataaagaat ttgttcaaaa tcatacacaa ggatttgatg agtataaaga
    23401 aattgttaaa ccatatacag ctaaatatgc tgaaaagatt acaggaatcc cagaagagct
    23461 tataagaaaa gctatgagag aatatgcaaa agggaaaaaa gctatgatac tttatggaat
    23521 gggtgtctgt caatttggtc aagctgttga tgtagttaag ggacttgcat caattgctct
    23581 acttacaggt aattttggta gagaaagcgt tggaatagga cctgtacgtg gacaaaataa
    23641 tgttcaagga gcttgtgata tgggagctct tcctaatgta tatcctggct atcaaaatgt
    23701 aactgatgat aagataagag aaaagtttga aaaaacttgg ggggtaaaat tatctcctaa
    23761 taatggttat agtttaactc aagttccaga cttagtacta aaagagaaaa agcttaaagc
    23821 ttattatata tttggagaag acccagtgca aagtgaccca gatgcatcag aagttagaga
    23881 agcattagat gaattggaat ttgtcattgt tcaagatata tttatgaata agacagcact
    23941 tcacgctgat gttatacttc cagctacttc ttggggagaa catgagggag tatatacatg
    24001 tgcagataga ggattccaat tgatgagaaa agctatagag ccacaaggag atgtaaaacc
    24061 agactggcaa attataagcg aaatttctac agcaatggga tatcctatga attataaaaa
    24121 tactaaggaa atatgggacg aacttagaca attatgtcca agtttcttag gtgctactta
    24181 tgaaaagata gaaactcaag gatgcgttca atggccttgt aagagtgaaa gtatggaaga
    24241 taaaggaact atgtatctgt atgaaggtca aaagttttct actcctaatg gtaaaggtaa
    24301 tttgttcgct gctgaatgga gaccccctat ggaagttgaa gatgatgaat acccatttag
    24361 tttatgtact gtaagagaag ttggacacta ttcagtaaga acaatgactg gtaattgtag
    24421 aacacttagt tcattagaag atgaaccagg acgtgttcaa ataaattcta atgatgctga
    24481 aaaattagga attgaagatg atgaattagt tagaattagt tcaagaagag gaagtgtaat
    24541 aacaagagca acagttactg atagagttaa ggaaggtgca acatatatga cttatcagtg
    24601 gtgggttgga gcttgcaatg aacttacaat agcaaattta gaccctatat ctaaaacacc
    24661 agagtataaa tattgtgctg ttaaacttga aaaacttgaa gaccaagaat tagcagaaaa
    24721 atgtgtaaga gaagagtatc aaagtttgaa agacaaaatg acagctacaa atatctaatg
    24781 atttattaga tttaagattt ataaaaggca gaaaggctaa tattttatga acaatatata
    24841 ctcaaaaact aaattacaag aaaatagtaa ggataaagtt tttgaaaata gttatagtct
    24901 agtggactac aaatatttaa attcttttaa tgtagaagta agcaaaataa atgaacatga
    24961 agtatcaata gataatgaag aaataattgc agaatatcca cttagtttta ttttaaatga
    25021 taaatatgta aatacatttt tatgtacccc atataattta gaggaactta tagtaggatt
    25081 cttgaaaaca aaaggataca tagaaaacaa aaaaagctta ttaaaatttg agataaatga
    25141 gagtgaacgt tttgcaaaag taactattca agagaaacaa aaaaatcaag atgagcaaat
    25201 tatattttta aattcactag actatataac atgtgcttct gtaaagagca atataaaaat
    25261 aaattatgat acgatttaca gtataatgga tagaaattta aattcatcag aattatttaa
    25321 aaatactggt ggggtacata gcgtatctgt atttaatttt gataaaaaaa tagtcacttg
    25381 tgaagatgta gcaaggcata atgcaatgga taaggctatt ggattttgtg ttttaaatga
    25441 cataacatta aaagataaaa ttgtggctgt aagtggaaga atatcattgg aaatgatatt
    25501 aaaagcagct atgactcaaa ttcctattgt agtatcaaaa tcagcaccga ctaatttatc
    25561 aatagagctt tctaaaaaat taaatataac gcttattgga tttgtaagag gaagacggat
    25621 gaatatttat gcaaatccac agagagttat caaaaagtga gaataagtat atgtgtacta
    25681 aaaaagaaga acaaaaatgt tcaaagtgtg aaatctgtgg agtattaatt gacgatactg
    25741 ataaggtgta taataacaca tacaatttgt gcaaaaaatg taaacgaaat ataagtactg
    25801 agaaaatagt gaaatatata aatatatatg atgaaagtaa gtagacatat gatgaaagta
    25861 actagagggt tggaaatata caaattgaaa ggtgggataa tatgaaaaat aaaataggct
    25921 cttttgtaat agcggaccca aacaaatgta caggttgtag agcttgtgaa gtagcttgtt
    25981 ttacaatgca taatcagcat aataatgtgg gttatacagt tggaacagtt gatatacctg
    26041 ttatacctag actttattta gtaaaaggtg acaatttctg tatgccaata cagtgtagac
    26101 attgtgaaga tgctccttgt ttaaactcat gtccacaaaa agctatagtc aaagaaaaca
    26161 atattatgtc tgtaaatgaa gaaaagtgta taggatgtaa aacttgtctg ctagcatgtc
    26221 cttttggagc tatagactta cttccacaat atgaagatgg taaagaagta gaacaagtaa
    26281 ttttagatga aaataagaag atagcttata aatgtgactt atgtaaagac aatgaaaaaa
    26341 tagcttgtat aagtgcatgt cctcaggaag cattaaaact tgttactcca atagatgaca
    26401 aaaaagctaa gaatagacaa gctgcactta gtttattgtt aacaaataaa taaaggatac
    26461 aggggtaggt gacgtttatg ataataaata tagataagga tttatgtact ggatgtagag
    26521 aatgttcaaa ggtctgtcca gtaaatgcta ttgaaggtga agagggaaag ccacaagaga
    26581 taaatttaga tagatgtgtt atgtgcggtc aatgtgttca aacttgtaaa tcttatgcct
    26641 cagttataga tgaaggattt gagtttttac aagagaaaaa acaagaaaga gagataccag
    26701 agtctataaa tgaacctata tttgcagcat acaatgtatg tgatatagat aaggtaaaga
    26761 aagctttaag tgacccaaat gtattcacta tggtccaatg tgcaccagca gttagagttg
    26821 ctttaggaga agatttcgga tattatttag gatacttagg tgctggaaaa atggcagcag
    26881 cacttagagc tttaaatttt gatagagtct acgatactaa ttttgcagca gatttaacta
    26941 taatggaaga aggtagtgaa ttaattaaaa gagtgacaga aggtggaact cttccaatgt
    27001 ttacatcttg ttgtccagct tgggtaaaat tcatggaaaa aaattatcct aaattaacta
    27061 atcatttatc ttcttgtaaa tcacctcagc agatgggtgg tgcaatattt aaaacttatg
    27121 gtgcagagat aaataatgta gatgcatcaa agatatttag tgtttctata atgccatgta
    27181 catgtaaaaa atatgaatgt gatagagaag aaatggattc tagtggatat agagatgtag
    27241 atgtagtatt aactactaga gaactagctt atttaataaa agacatggga attgatttta
    27301 aaaatttaag agaagaaaaa tttgatagtc ctttaggtag ttatagtgga gctggaacta
    27361 tctttggtgt tactggtggg gttatggaag ctgctataag aacaggatat gagcttataa
    27421 ctggagaaag tattcctgat gtagaagtaa aacaagtaag aggagaaaat ggttttagaa
    27481 agtcaactat taaaatagga gacttagact taagagttgg agtagtaagt ggattaaaaa
    27541 atgtaatacc agtgcttgaa gacttggaaa aaggaaaatt agatgtagat tttattgaag
    27601 ttatgacttg tcctgttggg tgtgtaagtg gtggaggtca accaaaaatt ttactagaag
    27661 aatataagga attggcttat gaaaatagaa caaaagcaac atacgtgcat gatgaacatt
    27721 tagcaattag aaaatctcat gagaatcctg atattaaaaa actgtatgat gaatatctgg
    27781 tagaaccatt aggagaaaaa tctcatcatc tattacacac tacatattgt ataggaaagg
    27841 aagtggctaa gtgatgaact attttgttat agcagaccct gataaatgca taggatgtag
    27901 aacttgtatg ataggatgtg tagttgcaca ttcagatgaa gatatttttt accaaaatcc
    27961 agatgaaata aattttaatc caaaactttc tgtcataaag acaaaagaag taagtgctcc
    28021 aatacagtgt agacattgtg aagatgctcc ttgtgcaaag gcatgtccaa atggtggtat
    28081 agttagggtt gggaacacaa taaaaataaa tgaagaaaat tgtataggat gtaagacttg
    28141 tatgttagca tgtcctatag gtgcaataga catagttaca ttaaaagatg tagatgaagg
    28201 aaagctttgc tttagagaaa gaatgacagc aaataagtgt gatttttgta tagaatcgcc
    28261 agaagggcct gcatgtgtaa atgtttgtcc aactaaggca tttactatag ttaaggatga
    28321 ggatatagat agtaaagtta aaaataagag aaaattagct atattgtaat ataaaaaaga
    28381 tggagtttta tttatgatta aaagctccat cttttttatt ataatattta tatatctaaa
    28441 aatcaattat tgcattagtt tatctaatct ttctttattc aattttttca ttcctataga
    28501 agttataata agtaaagtat aggcaattat aactgataaa attataatag accaagaaaa
    28561 tctgtatcca aaattatcaa ctaacaatcc aaataatgtt gaacctaatg caaatcccac
    28621 tgcaaaaatt aattgtacga ttccatatat gctaccaaac tccttttgac caaagaaaga
    28681 gccagttaaa taagcaggac caatcatata agcaaataca gaaagacctt tcaaaatggc
    28741 aaatacaaat gcaaaagtag aagatttatc taccaatagt aatgataaag ttgctataag
    28801 aaccaataca aaagatataa taagtggctt tgtttgaccg aatttatcaa acaataatcc
    28861 tccaaataaa ttaccaatga gagcaaataa agcaaatgtg gagcctactg ttcctattag
    28921 aataggattt agataatttc taagataagt tggatattgt acggataatg atgaaacata
    28981 tataccaaca aatataaatc caatagtcat aatccaaaaa tatttaattt ttttagcttc
    29041 ttttaatgta taaccccata ctgaaggaga aattgtatca gatgctttct cttttttacc
    29101 tttagatttt accttttcat taatatcttt aggcatcctg agtatgaata atattacagg
    29161 tataccaact attaatgcta taattccaaa tattagataa gaatgactgt atccttgagt
    29221 tgctaataag cgaactgcaa gtggttgtag aaatatacta ccaatagaac caccagcaaa
    29281 agcaaagccc aaagcttttc ctttgctttc ttcatcaaac caagcactta ttaaaacagg
    29341 tattcctata gcagatataa ttgtagcgcc aatttgaaca atacatgata cagtgtaaaa
    29401 ttgccatagt tcagtacata tagaaaaagt agcaaaagca atactagaaa ttatacaacc
    29461 taataaatat agtatcttaa tattgacttt agaaaataat acacctataa aaggtgatgc
    29521 aattgctgag gcaatagtac caatagtgaa tattagagaa aaagaagcta aactaaagcc
    29581 ttctccttct acaacataac ttataaattg aggctgtata cttgctacaa tactaaatgg
    29641 aacagcttgt attaacatac aaccaagtac tataacccaa gcatttgaaa agtttttaat
    29701 gctaaattta ttgtttagta atgtattcat ggtatctctc ctttaggtta tgatttgtag
    29761 atattaatct aattattcta attttatata ttagttttgg atagcctaac tatttaaatg
    29821 taatttgggt attaatataa aaattaacta taaaaattga ttattttaaa tgtatggggt
    29881 aaattaaagt atataaataa atgaaaggat ggataaaatg tcatttgaaa aaataaataa
    29941 taataaccta gataaaatac acaataggaa gtgtattatg cttgtaaact ttaataaaaa
    30001 agaattatca ttaattaaaa acgtaagtaa ctttgctgga ataagagacc atataatctt
    30061 aagtgaaaaa aacggagaga atataataag agacatttta gatggaaata tatcaagtga
    30121 atgtgaaaat gggattaaga gtaagtctgt aatatttaat aacactccaa gcacacaagt
    30181 aaatgcattt ttagaaggtc taaaaaaagt gagaataaat agacctttta cagctatggt
    30241 aacagagaca tctattgatt ggacattgaa taatttaata gaaaatttag ttcaagaacg
    30301 taatgctatg tctttaggaa aaacaattga acataagtaa atttaagata tcttatagta
    30361 attttaacta atgtacattg agtatcatat tatattttag aatataaaat tttaaatgtg
    30421 ggtagataga ggagatttga gatgaaagtt ttatttacaa taaattatgg aaaagaaaaa
    30481 tttgataaaa taagagaatt aggctatgat gtcatacatt actctgaaga tagtattgaa
    30541 aataatgagg atgtaaatac agcagatgta ctagttacat acaatccatt tgagagatta
    30601 gatatttcta agatgaaaaa tttacaatat atacagacaa ctagtgtagg tatagaccaa
    30661 cttccaaaag aagaggtatt gaagagaaat attaagatag ctaataatag aggcggatat
    30721 agtgtaccta ttggtgaaag tattgtattg tatattttag aaatatataa aaatagtgca
    30781 aaatttttta aacaacaaca aaataaagaa tggaagatgg atttttctgt atcagaattg
    30841 tatggacaaa gagttggatt tttaggaaca ggtactatat cttcagaagc agcaaaacga
    30901 ttaaaagctt ttggtgtaga aatatggggt gtcaatactg atggaagtag taaaaaatac
    30961 tttgataagt gttttgctac tgaagatatg aatattgtat ttgaagaatg tgatatagta
    31021 gttgtagcaa tgccttctac aaaaagtaca gatggaatag taaacaaaga tatgtttaaa
    31081 cttatgaaag atggttctgt atttataaat gtaggtagag gaaatacagt taatcagaaa
    31141 gatttagaag aatgtgtaga gaaatttaga ggggtagcac tagacgtatt tgaaagtgaa
    31201 cctttaaaca aggacaataa gttgtgggag tgcgaaaatg tcatagtaac tcctcataat
    31261 tcttgggtgt cagataaaaa caaagagaga acatttaata tggtttataa taatttgaaa
    31321 cactatataa tggagaataa acctgtagat aatgttgtag acatatcaag aggctattaa
    31381 aaataaaaaa ttttgataaa aataaaaact cttattagtc gtattaaaga tactaataag
    31441 agtttttttt gtaataaatt taaagtcaaa aaagtgatat actaaaaaat atataaatag
    31501 gagataatta attataggga aatttcaaaa taatttaatt ttaaaaatat gaagaggtgc
    31561 aaaaagtgag cgtaaaagat gaaattaata aacttataga gacaataaac taccataatg
    31621 aaaggtatta taatcaagat agcccagaaa tatcagatta tgaatacgat aagttaatga
    31681 aggagttaat ttctttagaa gaagaacatc cagaactaaa gagaattgat tctccaacaa
    31741 atagggtagg aggtaagcca ttagataaat tcaatcaaat tgtacataaa actcctatgt
    31801 tgagtttatc aaatgctttt tcagagcaag atttgagaga ttttgataag agagttcaag
    31861 aatatgtggg agaaaatgtt gaatatgttg ttgaatttaa aatagatggt ctttctgtag
    31921 gtctaactta taacaatgga gagtttgaaa aaggagcaac tagaggagat ggagttgtag
    31981 gagaagatat ttctcaaaac ttaatgacag taaaaagcat acctcttaag ataaatgata
    32041 aaaaagaact aatagttaga ggagaagtat atatatcaaa agataatttt agaaaagtaa
    32101 atgaacaaca agaagaacaa gaacagccat tatttgctaa tccaaggaat cttgcagctg
    32161 gttcattgag acaattagac ccaaaactaa cagcaaagag accattagat atatttgtat
    32221 ttaatatgga aaatatagaa gaatatgatt tagaaagtca cagtgaatca ctagagtact
    32281 tagaaaaact aggattttca gtaagtcaga gttataaagt ttgtaaaagt atagatgaag
    32341 ttattgagca tattgaatac tggacagata acaggggtaa tttagactac gaaatagatg
    32401 gtatggtaat aaaagtaaac aatataaaac aaagagacga aatgggatat acagccaaaa
    32461 gtcctagatg ggcaattgca tataaattcc cagcagaaaa gaagaaaact aaaatagtag
    32521 atataatagt agaagttggg agaactggta caataacacc ttcagcaatt cttgaaccag
    32581 tcagacttgc aggtactact gtaagtagag ctacacttca caatgaagat tatataagag
    32641 aaaaagatat aaaaataaat gatacagtat tagttcaaaa agcaggggac ataatacctc
    32701 aagttttaga agttataaaa gaggaacgta ctggagatga aatagatttt aaaatgccag
    32761 aaaagtgtcc agtttgttca gagccaacaa taagattaga aggagaggct gctgtaaaat
    32821 gtatcaatat gtcttgccca gcccaaataa gaaggggaat tatacacttt gtgtcaaggg
    32881 atgcaatgaa tatagatgga ttgggagaat ctataattac actgctatta aatgaaaaaa
    32941 ttattcaaga tgtatctgat ttatattata taaaaaagga agatgtagtc gacctagaga
    33001 gaatgggcga aaaatctgct gacaatttaa taaattcaat tgaaaaatca aaagaaaatg
    33061 atttatggag acttataaat ggattaggta ttaaatttat aggagttaaa gcagctaaga
    33121 tacttgcata taactttaag gatttagatg aagtaatttc tgcaactagt gagcaactag
    33181 aagaattaga ggaatttgga agtataatgg caaacagcgt tgtcgagttt tttaaggaag
    33241 acaaaaatat gaacgttata aacaagttaa aaaatgtagg agtaaataca aaatcattgg
    33301 ataatacagg tgaaaaagta gaaaaaatat ttgaagggat gaaaatcgta ttgactggta
    33361 cacttcctac attaaaaaga aatgatgcga aagatatgat agaaaaaaga ggtggaaaag
    33421 ctacatctag tgttagtaaa tcgacaacat ttgttttggc aggtgaagaa gctggttcta
    33481 aacttactaa agcaaatgat ttaggaatta aagttataga tgaagagaga tttttagaat
    33541 tattaaagtt atcatcgaaa gatgaagtaa aagctgtatt agagagttaa aaagtcgctt
    33601 aatttagtag aaaactacta taattaatga tagaatagtt cagtaagcta ataagattac
    33661 gaaaaatgac aataattaat atttgacaaa attaaaaatt ataaaaataa atttataaaa
    33721 aataaaaaat atgagaaagg atataaaaaa tgaccagaag ttatgataga agtaacgaac
    33781 agataagacc tgtaaagata actagagatt atacaaagta tgcagaaggt tctgttctta
    33841 tagaaatggg agagacaaag gttatatgca ctgcatcagt ggaagaaaaa gttcctcctt
    33901 ttttaagaaa tagtggtact ggatggataa atgctgaata ttctatgcta ccaagagcaa
    33961 cacaacaaag aaagataaga gatgcatcta ggggaaaaat tgatggaaga acacaagaaa
    34021 tacagaggtt aataggaaga gcaataaggt ctgttgtaga cttgaataaa ttaggagaaa
    34081 gaactatttg ggtagattgt gatgtgattc aggcagatgg tggaactaga actgcatcta
    34141 taacaggggc atttgtagca gttgcagaag caatatataa attatataaa gcaaagacaa
    34201 taaaagagat gccaataaca aattttgtat ctgcaattag tgttggtatt gtaaatggaa
    34261 tacacatgct agatttatgt tatgaagaag attctaaagc acaggtagat atgaatataa
    34321 taatgactga taagtgtgag tttgtagaag tacaggggac aggagaagag aaaccttttt
    34381 caagagcaga tttaaattta ttgttagagt taggcgaaaa agggaataaa gaattaataa
    34441 ggcttcaaag agaagcttta ggtgaaatag cagatgagat attgggtatg gattatggta
    34501 atgaagtagt gattgctact aataatgctc ataagctaga agaaataggt gaaatcttaa
    34561 aagattttga atataaagtg tactctttaa aagatgttga tttagcaggt atagagatag
    34621 tagaagatgg aaaaacattt gagcataatg cgttgataaa agctagagca atagcaaaaa
    34681 agactaaact tatagcaata tcagatgatt ctggcttaga agtagatgct ttaggcaaaa
    34741 aacctggtgt ttattcagct agatatgcag gagaacatgc aacagatgaa gaaaatagaa
    34801 aaaaactgtt aaaagctatg caaaatgtac caatgagcaa aagaaatgca agattcgtct
    34861 ctgcaatagc agtagttttt cctgatggaa aagaatttgt agtaagagga atttgtgaag
    34921 gaatgatagg atttgaagaa aaaggtaaaa atggttttgg atatgatagc ttatttatag
    34981 taaaaggata tgataagact tttggtgaaa tacctagtgt tataaaaaat tcaatcagtc
    35041 atagagcaaa cgcactaaaa cttatgaaac aagagtttat aaaaagggta gtgaagtaat
    35101 gaaaataggc atagtaagcg acacacatat gatgattaaa aatatggaaa aaacaatacc
    35161 atatttgaaa gaatgtgacc taataattca tgcaggagat aactttactg attcaagata
    35221 tattcatagc atgactaatg taggtattat tgcagttaaa ggtaattgtg actttgatgc
    35281 tgttgaggaa gaagttgtct ttgaagtggc aaataaaacc atattcttat gccatggaga
    35341 taagtatggt gtaaaatatg gaactaatat gttagaaaaa aaggcaactg aagttgatgc
    35401 tgatatagtt atatttggcc atacccatac cccatttaga gaaattaaag atggtgtact
    35461 ttacataaat cctggaagca cttcacttcc aagaggagta tcgtataaaa gttttgtcat
    35521 tatggatata gaagaagatg acataaaaat tgaagaaata cgcatataaa ggtgtattta
    35581 tctaaaaaaa tggcaaaaaa cgctcaaaaa attaaatttt atcaaataag tacaacgata
    35641 ttaatggaac acttgtaaat aaaaaaactt atgtgatata attaagtggt tgaattataa
    35701 atagaatgaa ttataatacc gttatcgggt attacataaa tatcactagg aggattagaa
    35761 ggatgaaagc agaactaatt aaaaaagagg gtaataaagt tactttaaaa ataacagtag
    35821 ataacaataa atttgaagca gcagtaaata aggcttataa taaaagcaga aataaataca
    35881 atatacctgg atttagaaaa ggtaaagctc caagaattgt tatagagact caatatggaa
    35941 aaggtatatt ctacaatgat gctatagaaa tattattccc agaagtttat ccagaagcta
    36001 tcaaggaatt agacatagac ccaatagaca atccagattt agatatagaa gaaataagta
    36061 aagataatgg gttggttatg gttcttaatg tagaagttaa accagagttt gaattaggaa
    36121 actacaaggg tatagaaata gctaaggttg agaatactgt aagtgacgaa aatgttgatg
    36181 ctaaactaca agaaatggtt gaaaaaaatg ccagattagt aagtgtagaa gacaaagctt
    36241 tagaagatgg agatacagct ataatagact tcgaaggttt tgaaaatggt gtagcttttg
    36301 atggtggtaa aggtgaaaat tataacttag ttataggttc aaacacattt ataccaggat
    36361 ttgaagaaca attagtaggt aaaaaagctg gtgaagaagt agaagttaat gttactttcc
    36421 cagaagaata tcactcacaa gatttagctg gaaaaccagt tgtatttaat gtaaaaataa
    36481 atgatgtaaa agtaaaagaa ttatctgcac ttgatgatga atttgctaaa gatactagtg
    36541 aatttgattc tttagatgaa ttaaaagcag atgtaagagc taagttagaa gaagaagcta
    36601 aaaatagagc agatgcagaa actagaaatt cagtagtaga aaaagttgct gaaaatacag
    36661 aaatagaaat accagaagtt atgatacaac atcaaataga taatatgtta aatgaactta
    36721 actaccaatt acaatatcaa ggatttggat tacaacaatt actagagatg actggtagaa
    36781 ctatggaaga attaagagaa gagagaaaag aagatgctaa aaaattagtt aagtcttctt
    36841 tagtattaga agcaataact aaagctgaag gaattgaagc aacagaagaa gaatttaagg
    36901 ctgagttaga aaaaatggct tctgcttaca atatggaagt agaaaaaata gaagcttctt
    36961 taagagatgc tgacaaagaa gatataaaag gtcaaataaa aataagaaaa acaatagatt
    37021 tattagttga taatgcaaca atagcttaat aaaataaacg ctaactaata tagttgacgt
    37081 tatgaaatgt ttagtatata atgatgtgca aatataattt agttaaatgc gtataataat
    37141 ttacagtagc ttaaaaaagc tactgtaaat tatatagaat aaagggggta tgaaaccatg
    37201 gcattagtac ctgtagttgt tgaacaaaca ggaagaggag aaagatctta tgatattttt
    37261 tctagactat taaaagacag aataatcttc ttaggagatc aagttaacga tgccacagca
    37321 gggcttatag tagcacagct attattttta gaagctgaag acccagacaa agatatacat
    37381 ttatatataa attctccagg aggaagtata acttctggaa tggctatata tgatactatg
    37441 caatacatca aaccagatgt aagtactata tgtataggta tggctgcttc tatgggagca
    37501 ttcttgttgg cagcaggagc aaaaggaaaa agactagcac taccaaatag tgaaataatg
    37561 atacatcaac cattaggtgg agctcaaggt caagcaacag atatagaaat tcatgcaaag
    37621 agaattttaa aaataaaaga aactttaaat gagatattat ctgaaagaac tggtcagcca
    37681 ttagaaaaaa ttaaaatgga tactgaacgt gataacttca tgagtgcact agaagccaaa
    37741 gaatatggtt taatagatga agtatttaca aagagaccgt agatagagag aggtgttaat
    37801 atgtctaaat atgaagaaaa acgacaatta aagtgttcat tctgcggtaa aaatcaagat
    37861 caagtaagaa gattaattgc aggtccaaat gtatatattt gtgacgagtg tgttgaactt
    37921 tgtgacgaaa ttattcagga agaaatagaa gatactatag atgaagatac tacatcttta
    37981 ccaaaaccaa aggaaatgat ggagattcta aatgattatg taataggtca agaaaaagct
    38041 aaaaaagctt tatcagttgc ggtttataat cattataaga gaatatacag taaaaaatct
    38101 agtagtaagg atatagaaat tcaaaagagt aatatacttt tattaggacc tacaggttct
    38161 ggaaagacat tacttgctca aactctagca agaactctaa atgtaccttt tgcaatggca
    38221 gatgctacat cattgactga agctggatat gttggagaag acgttgaaaa tatactttta
    38281 aaacttattc aagctgctga ttttgatata gaaaaagctg aaagaggtat aatatatata
    38341 gatgagatag ataagattgc aagaaaatct gaaaacccat ctataactag agatgttagt
    38401 ggtgaaggtg ttcaacaagc acttctaaaa atattagaag gtactgttgc aaatgttcca
    38461 ccacaaggag gaagaaaaca tccccatcaa gaatttttaa agatagatac tactaatgta
    38521 ttatttatat taggtggagc ttttgatgga ttagagaaga taattcaaaa acgtggtgga
    38581 gataaaacac taggttttgg agctaaaata gaaagtaaaa aagaattaga tttagggaag
    38641 ctgtatgaaa aagtacttcc agaggattta ttaaaatatg gtataatacc agagtttata
    38701 ggaagaatac cagttttagc tactttagag ttgttggatg aagatgcatt aatgcagata
    38761 ttacaggaac ctaaaaatgc tctagtaaaa cagtataaaa agttattgga gttagatgat
    38821 gttgagctag aatttgaaga aggtgctctt cgtgcaatcg ctaaaaaggc tatagaaaga
    38881 aatacaggag ctagaggact tagaagtata gttgaaagcg taatgatgga aactatgttt
    38941 gaagtaccat ctagagataa tataaagaag gttatagtaa cagagaaatc tgtgaacgaa
    39001 gactcagtaa atcctatcat agtacttaag gatcaagagg aaagtgcata agatgatgaa
    39061 aaggagcttt aaaagctcct ttttttatgt taatatgcta aatataaaat aggtatcatt
    39121 atcataaaaa tgtgagtaat atacctagaa taccaaagac gaatacaatt gcaatggaac
    39181 caatttttcc tttatataaa agataaaatg atgctataaa tgcaattatt ccatataaat
    39241 cccaattaac ttctttggca gatgaaaaag ctgctgataa tatgaatcct agagtaattg
    39301 gtctaagaac tgtcaataag ttttctacct ttttattatt tttaaattta gaaaataact
    39361 ttgatataat agttagacct attaatgcaa aaattgtaac accaatagtt gcagctagag
    39421 aaccaaagaa gccagcagtc ttaaatccta caaatgtagc actatttatt gctacaggac
    39481 ctggtgtaga ttgtgatata gcaagtaaat ccaaaaactc ttgattagtt ataaagtgat
    39541 atttagatat aaattcttgc tggataaatg gtagcatggc ataaccgcca ccaaaactaa
    39601 atgcccctat tttaaggaat attaagaaga gtagtattat ttctgacata gactatatac
    39661 acctcctcca attattaaat aaatagggct tatattgaat acaccaacag ataaaactgc
    39721 tataattaat agtagtatgt tagttttaga ctttggtagt ttcttgaaca tgctcgtaaa
    39781 agaatacaat ataagagcta ttacaacagg cgcaactcct ttaaaaaatc catctaaaat
    39841 tttagatgat gaatttttaa agtatagata cgataagact aagactatac aaaatgatgg
    39901 taaagcagag cctaaggtac atataagaac tcctggtata ccataaagtt tatatcctaa
    39961 taatacagat atattaactg ccagtactcc aggataactt tgagctatgg ataaataatc
    40021 aataaattct tcctgctcta aaagtttttg tttgtttaca agttcatctt gtataagagg
    40081 aaccatagcg tatccaccac caaatgtaaa cgcaccaatt tttaaaaagg ttaaaaacaa
    40141 tttcaacata atagaaacct ccaaaagata aataacatct aagtatacta atattaatat
    40201 tatatcataa taaaaagata atatagtatg atagtattct aagaatttat cattaatgtt
    40261 gaaaattaac ttgttcaaat atataatatt aaaagcaaag caattagact agaaaaggag
    40321 taatgttgat ggaacaaaat tatactaaaa tagatcatga attaccatta attcctctaa
    40381 gaggattagc tatatttcca tatatgatat taaattttga tataggtaga gagatatctc
    40441 ttaaagcttt agaccaagct atgatggatg aagaacttat atttttaact tctcagaaag
    40501 aagcagaggt agatgagcca ggagaagagg atttttatca tgtgggaaca atctgtaaag
    40561 ttaagcaaat gataaaatta ccaggagata cagttcgtgt tttggttgaa ggtgtatcaa
    40621 ggggtagagt aaagaaaata gaacaagaag atgggtattt cagagcagta atagaagaaa
    40681 tagtatttaa tagtgataat ttggatagtg aaacagaagt tgaaattgaa gcatttgtaa
    40741 gaaatgtatt tgatgccttt gaagagtata taaatattgg aaatagggta tcaccagaaa
    40801 tattaatatc tctagctgat atagaagatg tagatagatt tatagatacg atagctgcaa
    40861 acatatatct aaaatcaagc caaaaacaag aaatattaga agaatttgat attagaaaaa
    40921 gacttgaact tatttattct atattattag aggaaataga tatattaaaa atagaaaaga
    40981 aaataacttt aagagtaaaa aaacagatga ataaggttca aaaagaatat tatcttagag
    41041 aacaattaaa agctatacag aaagaattag gtgaagaaga agatataaat tctgaagcag
    41101 atgagtatag agaaaagctt aaaaaaataa aagcacctaa gacaaccaaa gaaaaaatag
    41161 aaaaagagat agataaattt tcaaaaatat cttctatgtc tccagatgta tctgtaagta
    41221 gaaactattt agatactata ttctcattgc catggaataa agagacaaaa gataaattag
    41281 atataactaa ggcaaaagat atattagatg aagaccatta tggattagaa aaagttaagg
    41341 agagaatact agagtatcta gctataagaa ctcttgctaa gtcacttaaa gggcctataa
    41401 tatgtctagt tggtcctcca ggaacaggaa agacatctat agtaaaatca atcgccagag
    41461 cattaaatag aaaatttgta agaatatctt tgggtggagt tagagatgaa gcagaaataa
    41521 gaggtcatag aagaacttat gtaggttcaa ttccaggaag aataattaat ggggtaaaag
    41581 aagcccaaac aaaaaatcct gtattcttat ttgatgagat agataagatg gcagcagatt
    41641 ataaaggtga cccagcttca gcaatgttag aagtattaga cccagagcaa aataaggact
    41701 ttgtggacca ttacttagaa ataccttttg atttatcaaa aatactattt gttacaacag
    41761 caaatagtct aggtaatata ccaagaccat tattagatag aatggaagta atagaagtat
    41821 ctgggtatat agaagaagaa aaattaaata tagcaagaaa atacctactt ccaaaacaga
    41881 ttaaagaaca tgctttaaaa gaaaacttta taaaaataga tgatgaaact ttaagaagta
    41941 taattaacca ttatacaaga gaagctggtg taagaacact agaaagaact attggtaaga
    42001 tttgtagaaa ggttgccaaa aaatacgttg aagacccaac tcttgaagag gttgttataa
    42061 ataagtctga cctagagaca tatcttggaa aagacatgtt taagtatcaa cttgcagaag
    42121 ttaatcctca aataggatta gtaaatggtc ttgcatggac agcagttggt ggagttacac
    42181 ttgaagtaga agtaaacgta cttaaaggta aaggagaaat tgtattaact ggaaaattag
    42241 gagatgttat gaaggaatct gcgaagacag gtatctctta tataaggtca atagtggaca
    42301 agtttgatat agaccctgaa ttttataaga cgaatgatat tcatatacac attccagaag
    42361 gcgctgttcc aaaagatgga ccatcagctg gtataactat ggctcttgca gtaatctcag
    42421 cacttactaa aagacctgtt cctggaaata tagcaatgac aggagagata actttaagag
    42481 gtagagtctt agcagtagga ggggttaaag aaaagttatt agcagctcat agagctggta
    42541 taacaaaagt attaattcct aaagaatgtg aagctgattt agatgaaata ccagaaaatg
    42601 taaaagaaaa aatggagttt gtgcttgtag aacatatgga tgaagtatta gaacaagctt
    42661 tattaaagag tggtgaaaat aatgaaaatt agaagttcag agataactat gagtgctgtg
    42721 aataaatcac agtatccagc agaaggaatc ccagagatag cattggctgg taggtcaaat
    42781 gttggaaaat catcaataat aaatacattg cttaacagaa gaaacttcgc tagaaccagt
    42841 caaacaccag gaaaaactag aactataaat ttttatctaa taaataatga attttatttt
    42901 gtagaccttc caggctatgg atatgcaaag atagctaagt ctgaaaaaga aaagtgggga
    42961 ggtataatgg aaagatatct agaaagtaga caagagttat gttctatatt cctacttgta
    43021 gatattagac atgagcctac agcagatgat aagcttatgt atgaatggat aaagcacttt
    43081 ggatataact gtgtagttat tgcaactaag gcagataaaa tatctagagg acaatatcaa
    43141 aaacacataa gtataataag aaaaaaattg caaatggaaa gtagtgaaaa agtaattcct
    43201 gtttcttcac taaaaaagac tggtgttgaa gaattatggg aagagattgt aaatcaatac
    43261 aatcaacatg gatatgaaat aacagtagat taaatttaaa atgttaaata ttgacataag
    43321 atttatttga ataataaatg taataatagt tagttttaat tatattaata ttaaagcaaa
    43381 atataggatg ccttaaaata gttttaatga actttttttg gcatccttat tgtttataaa
    43441 caataaatat cagtaatttt gctaaatact aataaatttt agtaaggtat gggaaaatta
    43501 gtataaatta gttcaactaa aaaattattg acttatgtag tcagcaataa atagcattta
    43561 agaaatagat ttatctgttt ttctcataaa gaaaacagtt agtataagcc ctataaaaca
    43621 tactgatgct gcaatccaat aagactgatt gctagctata acataggctg atgtgtgact
    43681 tttaaacttt tgattgtatg taagactttg tgcaaaaaaa ctactaaaaa atgctgttcc
    43741 aagagcaaat gataaattac gaatagttgc aagaaaactg cctccgtacc cctgtttttg
    43801 aggaggaaca tctgctaaaa tactactatt gtttggtgaa ctaaatgctc ctagcccaag
    43861 tccaaaaata actaaacata caattaaaag atacaatgga gaatctgctt ttaaaagtgc
    43921 cattccaact agtgagatgg tcataaatga aaaagatgct gttagaatgt tttttgcacc
    43981 gtgtttatca gaaagtgaac ctgcaatagg tgctataagt accataataa gtggatgtac
    44041 agtcattaca taaccagcca tcataggaga taaatttttt aattgttcta aataaaatgg
    44101 aagtagaaaa gatactgcca tttgaggaaa ataacaagct actccaatga tattgcctac
    44161 agtaaagttt ctatttttaa atagaggtag gtcaataaag ctttgttcaa attttacttc
    44221 tcttaatgcc aacaataaga agattataag tgctgttata cctaaataca atcttgtatt
    44281 acccttctca ttcattgcaa gtaatataag cgtagtgcaa gatgcgaaaa gtagaagacc
    44341 tgaaatatca aagctcttgt tttgttcatc caaaacaggt ttaggcagaa atttaatgcc
    44401 aagaaagact gctataatac caataggtat attgataata aaaatagaag gccatccaaa
    44461 atgttctaaa attatacctc caattacagg accactcata tttcctatac caactacagc
    44521 tccagtgatt cctatagctt ttcctttttc atttgctgga aatgcattgg aaactatacc
    44581 aagcccagta gccattaaaa tacttgctcc aactgcctga aagattcttg aagaaattaa
    44641 tgttgataaa ttgtttgaca tactacacag aaaagaacct attgtaaata taaaaaatcc
    44701 taatgcgtat agacgatttg aacctacttt atcaccaagt tttccgaaaa ataacatagt
    44761 agctgttaca acaagcatat aagctgttac aacccactga atttgtgaca tgttaatttt
    44821 aaagtcagca gcaattgtag gatttgcgat atttagtata cttccatcaa gagtagacat
    44881 aaatacacca atacagatga taatcaatac tatccatttt tgtttcatga taacactcca
    44941 atcttaatag attttataag tacgatataa ttgtaaaata aacaacaaaa aaagcacctg
    45001 tatattaggt accttgaatg tgtctaattt catgaataaa tacggtagtt gaaggatttg
    45061 tatagattac tcatgtaaaa ttacataaag gtactcatat actattttct tctaccatcc
    45121 agattttact gtcggctttg gaatatgacc aaattatgcc ataaggctcg cgggctttta
    45181 ccgccgatag ggaatttcac ccgtttatga agaaaaacag atgtattttg taatattatt
    45241 ttaacataaa attataatgg aaacaattgg atttaagtaa tatccagctg tattacaata
    45301 caaatatttc aatatggaca cttaaattgt atttatagat agaataaggt acagtatgag
    45361 aatctataat aattagataa aattaatatg ttattagtat tagatggtaa gttttaaatt
    45421 tgtcaaaatc agaaagctgt aataaaattc ttataatatg aatatcttaa ttaagagata
    45481 ttgtaaatta tgaggaggcg ctaatatgga aggaaaggta aagaaggcat tcccaggtgg
    45541 aaatacatca caaggctcat attctttttt tgactatata attcctgaaa atgtaaaaag
    45601 agttttttgt ttaaagggtg gacctggagt aggaaaatcc tcacttatga aaaaaattta
    45661 taaagagttt ttgcatagag gatatgatat tgatttatat cattgtcctt ctgacccaag
    45721 ttcgttggat ggtttagtta taaagaaatt aggcgtggta cttatggatg caactgcccc
    45781 acatactatg gacccaaagc tacctggagc tttggatgag ataattaatt taggtgatta
    45841 ctggaataca gaagaattag aaaagaataa acaagaaata tcagtttatg ataaagatat
    45901 tagtaattca tttaaaagag catttaagtt tttaaagtca gcagaaccaa tttttagaga
    45961 catagaagaa aaatattctg attgtatgga ttatggcaaa gtaaatttgg tcacagaaga
    46021 atttataaaa aggttatttg atggagttaa aagcacagga aatctaaaaa gagaaaagca
    46081 tttatttggt tcagctataa ctcctgtagg gtgtttagat tatacagaaa acattttaaa
    46141 agatgtaaag aaagtctatt atttagatgg agaaatagga actgggaaaa ctacattatt
    46201 aaacaaagta tataagaaag ctactgaaaa aggtttaaat gtagaagttt atcattatcc
    46261 acttattcca gagaaaattg aaactgtact attaactgat ttagatatag gcataactat
    46321 aagtacgact tttaaagaag aagatacaat tgatttaaat cagtttttaa atagagaaaa
    46381 actacttaaa tatgaagaag atattaaatt tgatgaaaaa gttttagatg atttaatttg
    46441 ttgggcagta ctaaatctta agagagccaa gtctaaacat gatattatag aaagttacta
    46501 tagtcctaat atgagatttg atgaagtagg gaaattaaga gataagttaa ttaaaagaat
    46561 tttaaaatat gaggaaaact tataaacgta tatgtttaaa attaatgtta ataataatat
    46621 aatttttagt aaattggtgt agctttgagg ctatgccatt ttactttttt ttgctaaaaa
    46681 atgatattaa taatctccaa agccattatt tgtttttcat aattattatt tcctttccta
    46741 atttatgttt ttttataata tcacaacaat gtttattgaa agtttatata attttaagaa
    46801 catctaaaaa tgcatacttg atattggtaa attagtgata ggagttcaat gtatgatatt
    46861 atatactaaa gatgaatttt aatatataac tttgatataa taagcatatg taaatgttta
    46921 atatttaatt aaagaaaatt atcatataaa aataaatttg taaggagcaa tacagtatga
    46981 aaaaacaata tgtgtttata atactagcaa gtactatttt aggtatactt gttggaacca
    47041 tatttagaga ttatagcaat gaagaaacag tttatacaag caaagacaag tcaataagaa
    47101 aagaaataaa aatgacaaga aaatctataa aagaattaac caaagagaaa gacaagttag
    47161 ataaagaaat taattcaatg aaacaaaatc aaaaaaattc tgaagaaata tcagaaatag
    47221 aaacacttag agatagtcta tcatattcag atgtaaaggg tagtgggata atattaagta
    47281 tagatgctat agatgaaaaa acagggaata tagctaatag tatagactat aataagattc
    47341 ttgtaaaagt tataaatgaa ttaaaagtaa acggaggaaa atacatagaa ataaatggtc
    47401 aaagaattaa tcaatattca gaagttgtac ttgcaggaag ccatataaat ataaatggag
    47461 ttcctgtagc acaaccatat gaaataaagt gcattggaga taaggagaaa ttatctaatt
    47521 atgtagataa aggtagtaaa tatttagata gcatacaaac caattatcca atgaaaataa
    47581 gcataaaaac tgaagaaaat atctctatga aaaaaatgaa tatacctaat aaattaaagt
    47641 atataaaggg tgattaatat tatattagag gaaagcagtt tgccaatgtt aataccagaa
    47701 aaacttgcaa agaaatataa tgtatttcca gttgagtttc aagatgataa tttagttgta
    47761 gaaatagaag atgaggatat ttatgctcta caggacttga gattagctac aggtaaagaa
    47821 attatattga agaaggagaa aaaagaactt ataagtgaaa agattgaaaa atattataat
    47881 agctttgaac ttgatgagga ttatgcaaaa aaattatttg aaaatctttt agaaaaagct
    47941 ataaaagaaa atgcaagtga tatacatata gaaccattta aagaacattt aatagtgaga
    48001 atgagggtag atggagaact aaaggaagta tcaaagtttt tagttgatgt ctatccatca
    48061 ttgtctacgg ttataaaatt aaaggcatca atggatataa cagaaaaaag gcttcctcag
    48121 gatggtaggg tggatataaa gttaggtgga aatttaattg acattagagt ttcatcaata
    48181 ccaactgtct atggtgaaaa aatagttttg aggatactta atagagatac ttttttaaaa
    48241 gacaaattag aacttggttt ttcagaggaa gctataaaat ctataaatcg tataataaat
    48301 aaaagagcag gagtattatt agtgacaggt cctacaggta gtggaaagac tacaacagta
    48361 tattccttat taaatgattt aaaaggaata aataaaaata ttatgactat agaaaatcct
    48421 gtagagtata agatggatgg cataagtcag attcaagtaa attcaaaagt agggttgagc
    48481 tttgatgtag gtcttagagc gatattaaga caagacccag atataataat ggtcggagaa
    48541 ataagagatg cagaaacagc taagatagca gtaagagcag ctataacagg tcatttagtt
    48601 ataagtactt tacatactaa tgatgcagta tcttccatag ctagattact agaaatgcaa
    48661 ataccaccat atcttttaaa tgcctctctt ataggtgtta tatctcaaaa gttggttaga
    48721 aaagtttgta atcattgtag ccatgagatt atgataaaag ataattttag tggagatgta
    48781 aatactaagg tagctgtagg gtgtgaaaaa tgtaatgata aaggatactt tggaagaaca
    48841 gcaatttatg aaatcttaga aattaatgat gatataaaga catgtataag aaatatggag
    48901 gattctagta ctattaaaga tgtggcacaa aataatggaa tgataacatt tgaagatagc
    48961 tgtaaaagac taataaatga aaaaataacg acattagaag agtgtttagt agtaaatgaa
    49021 atgatttaac tgaattgtat agttaaactt cgtttggtca gttgtcttta tttaatctat
    49081 tttaattaag atgtttgagt ataaattata tttaagtgaa ataaaattaa atgcattaaa
    49141 ttagaaaata ttaagtttat ttaattgtaa ttaatataat ttggaaggta ttatagtaca
    49201 tgagattatc taaaaatata ttttataatt tagaaaagtt ttatttagta ttaaatagaa
    49261 tcagtgataa agaaattaga gtaatttgca aagagatagg catattgctg gaatctggat
    49321 gtgaaattac aaaaatattt gaaatcattg agagtcagtc aagtaaaaaa gtaaagaatt
    49381 tgcttagtat agtatcaaat catattcaaa aaggtaactc aatagcagaa tcatttcaaa
    49441 taacaggtat attttctaaa ttctttataa gtatgataaa agcaggagaa actagtggaa
    49501 acttagatat aataatgagt gatttatcta attattatga taaagaatac aaattaa
//
LOCUS       DAEPOG010000009        46008 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEPOG010000009 DAEPOG010000000
VERSION     DAEPOG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.009"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            277..1971
                     /locus_tag="KR432_000451"
     CDS             277..1971
                     /locus_tag="KR432_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBH0639006.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2303..2413
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2517..2888
                     /locus_tag="KR432_000452"
     CDS             2517..2888
                     /locus_tag="KR432_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBH0639007.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2984..3931
                     /gene="trxB"
                     /locus_tag="KR432_000453"
     CDS             2984..3931
                     /gene="trxB"
                     /locus_tag="KR432_000453"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0639008.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            4016..4333
                     /locus_tag="KR432_000454"
     CDS             4016..4333
                     /locus_tag="KR432_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBH0639009.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4602..5888
                     /locus_tag="KR432_000455"
     CDS             4602..5888
                     /locus_tag="KR432_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0639010.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            6000..6473
                     /locus_tag="KR432_000456"
     CDS             6000..6473
                     /locus_tag="KR432_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6132..6134,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBH0639011.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6505..7815
                     /gene="grdB"
                     /locus_tag="KR432_000457"
     CDS             6505..7815
                     /gene="grdB"
                     /locus_tag="KR432_000457"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7552..7554,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0639012.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            8072..9604
                     /locus_tag="KR432_000458"
     CDS             8072..9604
                     /locus_tag="KR432_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBH0639013.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9615..10760
                     /locus_tag="KR432_000459"
     CDS             9615..10760
                     /locus_tag="KR432_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBH0639014.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            11070..12149
                     /locus_tag="KR432_000460"
     CDS             11070..12149
                     /locus_tag="KR432_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0639015.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12262..12861
                     /locus_tag="KR432_000461"
     CDS             12262..12861
                     /locus_tag="KR432_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639016.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(13048..13920)
                     /locus_tag="KR432_000462"
     CDS             complement(13048..13920)
                     /locus_tag="KR432_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0639017.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14337..15740
                     /locus_tag="KR432_000463"
     CDS             14337..15740
                     /locus_tag="KR432_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639018.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15767..17311
                     /locus_tag="KR432_000464"
     CDS             15767..17311
                     /locus_tag="KR432_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0639019.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17342..18733
                     /locus_tag="KR432_000465"
     CDS             17342..18733
                     /locus_tag="KR432_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBH0639020.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18926..20422
                     /gene="abfD"
                     /locus_tag="KR432_000466"
     CDS             18926..20422
                     /gene="abfD"
                     /locus_tag="KR432_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBH0639021.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20557..20832
                     /locus_tag="KR432_000467"
     CDS             20557..20832
                     /locus_tag="KR432_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639022.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20856..22163
                     /locus_tag="KR432_000468"
     CDS             20856..22163
                     /locus_tag="KR432_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0639023.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            22183..23301
                     /locus_tag="KR432_000469"
     CDS             22183..23301
                     /locus_tag="KR432_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0639024.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23588..24508
                     /locus_tag="KR432_000470"
     CDS             23588..24508
                     /locus_tag="KR432_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBH0639025.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24623..25780
                     /locus_tag="KR432_000471"
     CDS             24623..25780
                     /locus_tag="KR432_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0639026.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25865..27364)
                     /locus_tag="KR432_000472"
     CDS             complement(25865..27364)
                     /locus_tag="KR432_000472"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH0639027.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            27960..29384
                     /locus_tag="KR432_000473"
     CDS             27960..29384
                     /locus_tag="KR432_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH0639028.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            29419..31548
                     /locus_tag="KR432_000474"
     CDS             29419..31548
                     /locus_tag="KR432_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0639029.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            31578..32000
                     /locus_tag="KR432_000475"
     CDS             31578..32000
                     /locus_tag="KR432_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0639030.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            32019..33170
                     /locus_tag="KR432_000476"
     CDS             32019..33170
                     /locus_tag="KR432_000476"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0639031.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      33276..33377
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            33560..34132
                     /locus_tag="KR432_000477"
     CDS             33560..34132
                     /locus_tag="KR432_000477"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH0639032.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            34196..34846
                     /gene="fsa"
                     /locus_tag="KR432_000478"
     CDS             34196..34846
                     /gene="fsa"
                     /locus_tag="KR432_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0639033.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            34998..37922
                     /locus_tag="KR432_000479"
     CDS             34998..37922
                     /locus_tag="KR432_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639034.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            38511..38969
                     /locus_tag="KR432_000480"
     CDS             38511..38969
                     /locus_tag="KR432_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0639035.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            39030..39305
                     /locus_tag="KR432_000481"
     CDS             39030..39305
                     /locus_tag="KR432_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639036.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            39364..40638
                     /locus_tag="KR432_000482"
     CDS             39364..40638
                     /locus_tag="KR432_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0639037.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            40768..41820
                     /locus_tag="KR432_000483"
     CDS             40768..41820
                     /locus_tag="KR432_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0639038.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            41820..42851
                     /locus_tag="KR432_000484"
     CDS             41820..42851
                     /locus_tag="KR432_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH0639039.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            42943..43755
                     /locus_tag="KR432_000485"
     CDS             42943..43755
                     /locus_tag="KR432_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0639040.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            43758..44678
                     /locus_tag="KR432_000486"
     CDS             43758..44678
                     /locus_tag="KR432_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0639041.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            44853..45299
                     /gene="rpiB"
                     /locus_tag="KR432_000487"
     CDS             44853..45299
                     /gene="rpiB"
                     /locus_tag="KR432_000487"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0639042.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            45338..46003
                     /locus_tag="KR432_000488"
     CDS             45338..46003
                     /locus_tag="KR432_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0639043.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
BASE COUNT        18276 a         5050 c         8668 g        14014 t
ORIGIN      
        1 ttaattacaa ataatggtat cgtttgcatt gttaatataa ataaatgtat attatatagt
       61 tagaagtttg aataaataga ctgatggtaa tttgttatat tagttagttt tgtgttataa
      121 aatattgtaa ttccattaat catatgttat tattcaacat gattataata taaagaatat
      181 ttaatatata taataattta taaaaaagta ttacatagta catgtaaatc tatatttata
      241 aatacaataa agtaaagttg taaaggagtg ctttaaatga agttttcaga gtttaagtat
      301 gaaagaccca attataatag tatgaaaaaa gagtttttga gttgtgtaga agatataaat
      361 aattctcgta attataaaga gcaacaaaaa aatatacata agattaattt attaagaaat
      421 aaaatagaaa cgttgtcaaa tatagcttcg ataagatata gtacagacac atttaataaa
      481 ttctacaaag aagaaaaaaa ttattgggat gaatatatgc ctttgtatga agaattaaat
      541 tcacattttt ataatgctat tgtaaattca aaatttaaat atgatttgat aaaagagttt
      601 ggagaacaat ttttcacaat agtagagtat tctttaaaaa gtttttctaa agaaataata
      661 agtgaattgc aggaagaaaa taaattgtgt tcagagtata ctagactttt agcttcagct
      721 gagataatgt ttgatgggaa tataagaaat ttatctggta tggggaaatt catgtattct
      781 aagaatagaa aaacaagaga attagcaaac aaagcatatt ataatttctt tgaagaaaat
      841 gaaactaaat ttgatgacat atttgataaa ttggttaaac ttagagataa gatatctaaa
      901 aaactaggtt ttgaagattt tgtagagctt gggtacgtta gaatgatgag aagtgactac
      961 agagaagata tgataaaaaa tgttagaaaa caagtattaa aatatgttgt tccaatggcc
     1021 aatgagcttt atgaaaaaca agctaaaaga attggtctag aatatttaag ttacatagat
     1081 gaaggcgtgg aatttttaac aggaaatgca agtttaaagg gagattcaag atacataatt
     1141 caaaatggta aaaggatgta ttctgaactg tcaaaagaaa caaatgaatt ttttgatttt
     1201 atgttagaaa atgaacttat ggatttagaa acgaaaaaag gaaaaggagc tggagggtat
     1261 tgtacttata taccagatta taagtcacca ttcatttttt ctaattttaa tcaaactgct
     1321 gatgatatag atgtgttaac acatgaggca ggacatgcat ttcagttgta tatgtctaga
     1381 tggattgata tgccagaaat taattttcca acgcttgata gttgtgaaat ccattcaatg
     1441 agtatggaat ttataacatg gccgtggatg gatttatttt ttaaagaaga tacagataag
     1501 tacaaattca ctcacttatc atcttcaatt aaatttatac cttatggagt aattgttgat
     1561 gaatttcaac actatattta caaaaatcct aatgtagata agtcaaaacg aaaagaaatc
     1621 tggagatttt tagagaaaaa atacttacct catagaaaat atggtgataa ttctttttta
     1681 gagagaggtt gttggtggtt taaacaagga catatattta aaaatccgtt ttattatata
     1741 gattatgtat tagctcaaat ttgtgcgtta caattttgga aaaaaatgat tcaagataga
     1801 gatgcaggat ggaaagatta tataaatatc tgtaaagttg gaggaacaaa atcattttta
     1861 gatattgtga gtatgggaaa tttgtattct ccatttgatg atggctgtat tgagtctatt
     1921 ataggtgatg ttaaatcttg gtttgatgaa ataaatgaca gcaaattata aaaaattatg
     1981 agaattttat tgtataaaag tgaaaaatta aaaaatatat aaaaagtgat tatttgttgt
     2041 gaaaatagtc ttgtttttaa ctaaattgat aaaataaact aaaaaagatt tgtacttaaa
     2101 aaaaaaagaa aatacaagaa atatgcaata tcaacatttg gaaaaaaaag taaaaatgta
     2161 tacaaaatac atataaaaaa actattgcaa attttataga atgtttatat acttaagtca
     2221 acagagatag aaaaaagcta tttatagaaa acaaaggata aattgcttaa ttttaaattt
     2281 tctgaaaaaa ataataatcc ggatgaagag tatgggagac tgctttataa aaagcgaccg
     2341 aagggacaag tttaatctta cttacccaaa agattagatg aaattctcag gtaaaggtga
     2401 ctgtacttgg acggggctct gaaaatgatt ttaacaggga agacaaattg ggagatttgt
     2461 ttttcctgtt ttttatttat caagtaataa ataaaagttt gaaagagagt aaaaaaatga
     2521 taataattac gaataatcca aaagtaaaag aagaagttca aggcagagaa gttttgttta
     2581 aagacacaac ttatattgga atcttagaag caagtagaga tttgatacat gaaggttatg
     2641 aacttttatc tcatccactc tatgggagtg taaaaccaaa tgaaacacct tacaggactg
     2701 ttatactaaa gaaaggaaat cgtttggata taaactcttt aaccttaatt gaagaagcaa
     2761 tcatcactgc aagtaaattt caggataata aaaagactcc aaaatggaca gaaaatgtcc
     2821 aagacgattt tagagtaatt gattatgata tattttataa tacaatacaa agaatgcagt
     2881 atgaataaaa aaatttgtta aaaagttatt gaaacagcct aaatattaaa tctaatttaa
     2941 tattaaataa aatgagaaat ttaattgggg aggataagtc aaaatggaaa atgtgtatga
     3001 cttagtaata attggttcag gaccagctgg gcttgcagca ggtctttatg gagcaagagc
     3061 aaagttaaaa actctaatcc tagaaaaaga caagactggt ggacaaattg taataacaca
     3121 tgaaattgca aattatccag ggtcagttcc aaatgcaaca ggaccaagtt taatagctag
     3181 aatggttgaa cagtgtaaag agtttggagc agaaatgcta agagataaca ttgttgatac
     3241 tgagctagat ggagatataa aagttttaaa aggggaaaag gcagaatata gagcaaaagc
     3301 agtaataata ggaacaggag caacaccaag aaagattggt tgtcctggag aaaaagaact
     3361 tactggtaaa ggggtttcat attgtgcaac ttgtgatgca gatttctttg aagattttga
     3421 agtatttgtt gtaggtggag gagacagtgc attagaagaa gcaatgtatt taactaaatt
     3481 tgctagaaaa gtaacaattg tacatagaag acaaggattt agatgtgcta agagtgttga
     3541 agaaaaagca aaagctaatc caaagataga gtttttatta gatacagtaa ttgaagaaat
     3601 caaaggtgat ggaatattag aatcagttgt atttaaaaat aaagttactg gagaaactca
     3661 tgaatacttt gcagatgaag aagatggaac aatgggagta tttgtgttcg ttggattaga
     3721 tgctcaaact gatttattca aaggtaaagt tgatatggat gaaaaaggtt acataataac
     3781 tgatgaagat atgagaacta acataccagg cgtatttgca gcaggagatt gtagatctaa
     3841 gactctaaga caggttgtaa ctgcaactaa tgatggcgca attgcatcta tagtagctga
     3901 gaaatacatt gatgaaaaat ttgggaacta gaatctgtaa attaagaaat agtgtctgta
     3961 aatttaatga ataatattta aaattaaaat aaaaatttca agggggaata agaaaatgtt
     4021 agatttagat aaggctacat ttgaagaaga agttttaaat gcagaaggtt ttgtatttgt
     4081 tgatttttgg agtgaaggtt gtgagccttg taaagcttta atgccagatg ttcataaatt
     4141 agctgagact tatggggata aaataaaatt ctgtaagatg gatacaacaa aagctagaag
     4201 attagctata aaacaaaaag tattaggtct tccaactatg gctatataca aagatggaga
     4261 aaaagtagac gaagttacta aggatgatgc tacagttcct aatatagaaa acatgattaa
     4321 aaaatatctt taatatgttg ataaagtatt aacattaaaa gaaaaaatct atttttatct
     4381 gaagataaga agaaaaaggt ttttatgtcc aactttgtta aaaaaataat tttaaataaa
     4441 ataatttttt tacaataaat attttttagt aataaaataa agatttaagg cataaaaaca
     4501 caataatata tctttaaaaa aataagataa attgtaaaat gatataattt tgagaaaaac
     4561 tcaaatgcat atacacaaat taaataacag gaggtgcaag tatgcgtctt gaattaggga
     4621 aaatctttat aaaagatatt caatttggtg atgtaacaga agtaaaagat ggagtattat
     4681 tcatcaataa gcaagagatg ctacaagaaa ttggtggaga tgagcatatt aaatctatag
     4741 atatagaatt agctcgtcca ggtgaaagcg taagaataac acctgtaaaa gatgttattg
     4801 agccaagagt aaaagttgaa ggtaatgggg gaatcttccc tggtatcatg tctaaagttg
     4861 atactgtagg agaaggaaaa actcatgctt taaaaggtgt agctgttgta actactggaa
     4921 aaatagtagg tttccaagaa ggtataatag acatgactgg agaaggagct aagtatactc
     4981 cattttctaa attaaataat gtagtagtaa tagctgaacc tattgatggt ttaaaacaat
     5041 acgctcatga aaaagctgtt agaatgatag gattcaaagc agcaatgtac ttaggagaag
     5101 tagcaagaaa tcttactcca gatgaagttt cagtatatga aactaaacct cttcttgaat
     5161 caataaaaga atatccagag cttccaaaag taggatatgt ttacatgctt cagactcaag
     5221 gtttattaca tgatacttat gtatatggag tagatgcaaa acaaatagta ccaactttat
     5281 tatatccaac agaattaatg gacggagcaa ttgtaagtgg taactgtgtt tctgcatgtg
     5341 acaaaaaccc aagttatgtt catataaaca atggggttgt tgaagactta tatgcacgac
     5401 atggtaaaga tataaacttt gtaggtgtaa ttataactaa tgaaaatgtt tacctagcag
     5461 ataaagaaag atcttcaaac tggacttcta aattatgtaa atatttagga ttagatgcag
     5521 ttatagtttc acaagaaggt tttggaaatc ctgatactga ccttataatg aactgtaaga
     5581 agatagaaat gcaaggtgtt aaaacagtta tagttacaga tgaatatgct ggacgtgatg
     5641 gaggttctca atcacttgca gatgcagatg taagagctga tgctgttgta acaggtggaa
     5701 atgccaacca agttgtagtt cttcctaagt tagataaagt tattggacat ttagaagtag
     5761 tagatgtaat agctggtggt tctgatggaa gtttaagagc tgatggaact attgaagttg
     5821 aaatccaagc tataacaggt gcaactaatg aaacaggatt tggtcattta actgctaaag
     5881 gatattaaga agtattttat taaacgattg agtaatgtta ataaaacata ttatagataa
     5941 ttataataag tgttatagat atataaaaat atattagaaa tataataagg aggattatta
     6001 tgagtttact tagtaataaa aaggttctta taataggtga ccgtgatggt ataccaggac
     6061 ctgcgataga agaatgtgta aaaacagtag aaggagcaga ggttgttttc tcatctacag
     6121 aatgctttgt ctgaacagct gctggggcta tggacttaga aaatcaaaac agagttaaag
     6181 atgctgctga taaattcgga gctgaaaatg ttgtgatttt actaggtgct gctgaagccg
     6241 aagctgcagg tcttgcagcc gaaacagtaa ctgctggaga tccaactttc gctggaccac
     6301 ttgctggagt tgccttagga ttaagtgttt accacgttgt tgaggaacca ataaaatcat
     6361 tatttgatga aagtgtatat gaagaccaaa taagtatgat ggaaatggtt ttagaagttg
     6421 aagaaataga agaagaaatg tctggtataa gagaagaatt ttgtaaattt taatgcctaa
     6481 aagcaaaagg ggtgaaacta agagatggga aaactaaaag cagtacatta tattaatcag
     6541 ttctttgctg gtataggtgg agaagaaaaa gctgatacta agccacatgt ggcagaaact
     6601 ttacctccaa taagtttaca acttgataaa ttattaggtg aagatattga aatagttgga
     6661 acagtagttt gtggagatag ctatttcaat gaaaatattg atagtgcaag tgaagaagtt
     6721 ttatcaatgg taaaaggttt tgaaccacaa cttttcatag ctggtcctgc atttaatgct
     6781 ggtagatatg gggttgctgc tggaacaata actaaagttg taaaagatgc tttgaatata
     6841 ccagctttaa caggtatgta tgttgaaaat ccaggagcag atatgttcaa aaaggatgtt
     6901 tatgtagtag aaacttctga ttctgcagca ggtatgagaa aggcacttcc taagatagct
     6961 aaacttgcag taaaacttgc taatggagaa gaaattggaa ctcctaaaga tgaaggatac
     7021 atagctagag gaataagagt taactacttc catgaagaca gaggttctaa gagagctgtt
     7081 gacatgttag ttaagaaaat aaaaggtgaa ccatttgaaa ctgaataccc aatgccaaac
     7141 tttgatagag ttgacccaag taaagctgta aaagatttat caaaatgtaa aatagcttta
     7201 gttacttctg gtggtatagt tccaaaagga aatccagata gaatagagtc ttcttcagct
     7261 tctaaatatg gaacttattc tatagcagga gttatggatt taacagaaga gacttatgaa
     7321 actgcacatg gtggatatga cccagtgtat gcaaatttag atgcagatag agtattacct
     7381 gttgatgttc ttagagattt agaaaaagaa ggtgtaattg gaaaattaca tgagactttc
     7441 tatacaactg taggtaatgg tacatctgtt gcaaactcta aaaaatatgc ttctgaaatt
     7501 ggagcagcat tagtggctga tggtgtagat gcagtaatat taacttctac atgaggtact
     7561 tgtactcgtt gcggtgcaac gatggtaaaa gaaatagaaa aaactggact tccagtagtt
     7621 catatgtgta cagtagtacc tatttcatta acagtagggg ctaatagaat agttccaact
     7681 atagcaatac ctcatccact tggaaatcca gcgcttgacc ctacagaaga aaaagcccta
     7741 agaagaggcc ttgtagaaaa agcattaaat gctttaacta cagaagtaga tggtcaaaca
     7801 gtatttgaaa aataaaaata acacacacat atattatata gataaaaata taaacacaca
     7861 ctaaaaataa aaaataacta tacacaaaaa aaataatata gatttcatga atggataaat
     7921 tggagttatt cattcatgaa atcttaaaaa atgccaaaat acataaacaa taatagaatc
     7981 aaatatacaa taaaatacaa tgattagagc ttttaataag tttaataaga ttaaaaaata
     8041 agtgttcata aaatttatgg aggtgcacaa aatgacttat ccagttttaa aaggggcagg
     8101 ctatgtactt attcatactc cagatatgat agtgcaaaat ggaagtactt gtacagttga
     8161 aagagcaaca aattcagatt cagaattttt aaaagaagta agtaatcata taagaagtta
     8221 tgaagatgta gttaactaca tgccaaatca agtttatata ggaaatagaa gaccagaaga
     8281 attaagagat ttaccaatgc catggtgtga acaaaagatt gagggaacta gaaatggtaa
     8341 gtttggagaa ataatgcctc aagatgagtt catagcttta atgcaaataa gtgatgcatt
     8401 tgatttagta aaattatcac aagaatttat tgatgaagta aagccaaaaa tcgaaaataa
     8461 ctaccctgaa attgcaccat ttgtaggtaa attaaagggt gatgatatag aagaaggtaa
     8521 agaattagta gcaacacaca tagctgaagg attataccat gatggaaaat ttgttggtta
     8581 tgtaaaaaga gcacatgatg tagatgttaa cttaaatgcc catacaatgt ttgaaaatct
     8641 agtagtaaaa gcatctggag ttttatctgc tattcaaatg ctaagacata gcaaaataga
     8701 ccctgctgaa atagattatg ttattgagtg ttcagaagaa gcttgtggag atataaacca
     8761 aagaggtgga ggaaactttg ctaaatcaat agctgagata gcaggacttc agaatgcaac
     8821 tggttctgac acaagaggat tttgtgcagc accaactcat gcattaattc aagcagcagc
     8881 tttagtaaaa gctggcatac ataaaaatgt aatggtagta gcaggtggag caagtgctaa
     8941 acttggaatg aatgctaaag accatgttaa aaaaggtctt cctgtacttg aagatgttgt
     9001 tggaggattt gcagtacttg tatctgaaaa tgatggtgta aatccagtta taagaactga
     9061 tttaacaggt aagcatactg ttggaactgg ttcttcacca caagcagtta tgacagcact
     9121 tataacttca ggtcttgata gagctaattt aaaaataaca gatgttgatg tgtattcagt
     9181 tgaaatgcaa aacccagata taactaagcc agcaggagca ggagatgttc cagaagcaaa
     9241 ctacaaaatg ataggggcac ttgctgttaa acgtggagac ttagaaaaga aagaattgaa
     9301 agattttgtt tctaacaaag gattaccagg atgggcacca actcaaggac atataccatc
     9361 aggagctcct tatataggat ttttaataga tgatttaact acaggaaata gaaatagagc
     9421 tatgatagtt ggaaaaggta gtttattctt aggaagaatg actaacttat ttgatggagt
     9481 atcttttata gctgaaagaa atacaggtgt tactgaagag acttcaggaa tatctaagga
     9541 tgaaattaag aagataatag ctgaatctat gaaaaaacta gcattagata tgttagaaga
     9601 ataggggtga ggatatgtca aaaaaagtaa tcgcagatgt attcttagaa gtagctaatg
     9661 caatagaaag cggcgaattt ggcaaaaaag taaaaattgg tgttactaca cttggaagtg
     9721 aacatggtgt agaaaacatg gttaatggtg cacaattagc aaaatctaat ttatttgata
     9781 ttgtgcttat tggaccaaaa gtggaaactg accttgaagt agttgaagtt aatgatgaaa
     9841 aagaaatgca tgcaaaaatg gaagaattat tagattcagg atatattgat gcttgtgtta
     9901 ctatgcatta taattttcca ataggtgtat ctacagtagg tagagtaata actccagcaa
     9961 aaggaaaaga aatgatactt gcaacaacaa caggaacaag tgctactaat agaatagagg
    10021 caatggtaag aaatgccatt tatggtattg ctacagctaa atcaatgggt aacaaatgcc
    10081 caaaagttgg aatacttaat gttgatggag caagacaagt tgagaagtgc ttaaaagaat
    10141 taaaagataa tggatatgac atggagtttg cggattcaat tagagcagat ggtggatgtg
    10201 taatgagagg taatgattta cttgtaggtg cacctgatgt tatggttact gatacattat
    10261 ctggaaatat atttatgaaa gtattttctt catatactac aggtggagat tatgaagctc
    10321 aaggctttgg atacggacct ggagttggag aagactatga tagaaaggta cttatagtat
    10381 caagagcatc aggttctccg gtagtagcaa atgctttaaa atacgcttat gatgttgtaa
    10441 agggagatat aagtaatgtt gcaagaaatg aatttgcaaa ggttaaaaaa gcaaaatttg
    10501 atgacattat atcctctctt actaaaaaag aagttaaagc tgaaaaagta gaagttaaaa
    10561 tgccagataa agaaattgtt actcgtcaaa tagctggtgt tgatataatg gatttagaag
    10621 atgcagtatc tgagttatgg aaaaacggaa tctatgctga aagtggtatg ggttgtacag
    10681 gtccaatagt tttagtaaat gaagctaaag gagacttagc agttgaaact ttagttaaag
    10741 ctggatatac tgcaaaataa gtacattttt tatatgtaaa taagaacata tagttataat
    10801 tttattgatt ataattatat gttcttttta ttttatatct tgtcaatatt tatatattaa
    10861 cacaatccat ttatttgatt ctctagctaa tttattatat ttattcaatt aaaattaagc
    10921 tttctaacta aataaaaact aatgaagtat taaaatgaaa ttaataaaat atgaaaaaat
    10981 tttaggaata aagtatctgt tagataaaaa taagattaat tagtgatata atataattaa
    11041 gataaaattt attatttgga ggtaaaatca tgagcagagt aaaaaatgta gtaggattgt
    11101 tgggaactaa aggtgttgat gcactatatc taacaaagaa aactaatgta aactatataa
    11161 gtggttttcc tgatgaagaa gcatatgcag tgatttgcaa agatggaaac tttttagtta
    11221 ctgatagtag atatatggag cttgcagaaa aggtttgcaa agattttgaa attataaatt
    11281 ggcataattt tgatagaagt gtggcaaagg cagttaaaag tgtatgtgat aaagtgggta
    11341 taaagaaact tgggtttgaa agaactaata tagtgtttga taaatatgaa gaacttaaaa
    11401 atctgattga gaaggataat ggagaattaa tacctacaga aaatattgta gagactttga
    11461 gatatgtaaa agacaaagat gaaatcaaaa atacaagaaa agcttgtgaa atagcggata
    11521 aggcattaga agaattgatt ccacatataa aagctggagt tagcgaaatt gaactggcta
    11581 caaagttaga gtactttatg aaaatgaacg gagcacaaaa tatagggttt gaaactatac
    11641 tgatatcagg agcaaagact tcattgcttc atggtaagcc tagtgataag attatagaaa
    11701 aaggtgattt tgtacttata gactatggtg ctatgtataa tggctatata tctgatacaa
    11761 ctagaacttt tatagttgga ggagcttctg aaaagcaatt agaaatatat aatttagtta
    11821 aggaagctca aaatgtaggg gttgagaaca tgaaagctgg tgtacatgcg acaattccag
    11881 atgctgagat aagaaaagtt gtcaaaaaat atgaagacta ttactatcaa ggtataggac
    11941 atggagtcgg tagggatgtt catgaagaac ctttcattgg taattatggt gataaaatca
    12001 tagaagaggg atgtataata acaatggaac caggtattta tttccctgga tggggtggag
    12061 ttagaataga agatactgtt ttgattacta aaaatggtcc agaaagactt actaaattcc
    12121 ctaaagattt gatgatttta gacaaataat ttaatttata tatatttttt tacataaaat
    12181 ataacataat ctcactatta agctctattt acatattata gtaaagaggg cttaatttta
    12241 ttgtaagagg tgatagtata tatgtataaa aaacataaat ttataaaaaa tataaaaaaa
    12301 gcctggtata aaatcaaatt aattgataga tgccttataa ttgtaatggc aatattaatg
    12361 tttcaatcta tatataattt atttgttaat gaagtaaatt cacaagatac tacaactatt
    12421 gatgtagtag taagaaccac ttcagcagct atatttgggt attttttaag tgctaatttt
    12481 ataaagagac ctttaaggaa gtatacaaat actgatgtat cttcaaatct ttttatagat
    12541 aataatttac ccccaaaagg tcaagagtct agtcaaaata atataatgaa tgtcaaaaat
    12601 accattggat ttacttcaga agctgataat tatgaaaaga aaatacctat taataataat
    12661 gaaggttttg aagaaggaga aactagtgaa ttgcaaatta ttatagctac tgtaatttgt
    12721 atagcagcat taacagtact ttttgttgta agaaatttta caactacaac atcagcttct
    12781 ttaggtacta tttcacagat gagagatttt gtctcaggat gcgtgggttt tttgctaggt
    12841 tgtccaagta aacctgatta aaaaagtatt atcaataaaa ggtattttaa aaagtatcag
    12901 tataataata tttagattta aatgtataca attataattc tataacattc tatattgtat
    12961 ttttatactg atactctaaa ctatactatt tattaaaata ttttaacatt gtatttaaac
    13021 aaaatattat atctgtataa tataaattta gtttatatga atagatgcca tatactcaat
    13081 aaaaagtttc atactatgag atatgtattg atttcttctc caaagaactc caatatttgc
    13141 agtaattgca cgttctaatg gtattgaaca gagattttct gtattttgaa atatttcttt
    13201 atacaatatt gttgaagcca agtcattttc tagtaaataa cgtatggtag aaagttgatt
    13261 tgagtgtaaa attatatttg gcttagcttt ttcaagtctt tttgtaataa tcttatagtg
    13321 ataagaacct tcagataaga ttacaaaagg tacatttttt aacatataat ttgatataat
    13381 gggatgtttt gccaattcat ttttgggatt tgtaactaag tacaattctg ttttatagat
    13441 tgtctttgaa tctatatcat cataaatatc actgtcaatt ataccaatac aaaaatcaag
    13501 tgtagattct tcaatcattt tagctccaac aaatgttgga acttcaaaaa ttttaagctc
    13561 tatatctgga taaattacat taaatctagg aagtattttt gaaagcaaaa acgtacctaa
    13621 aactgtagga atacctattt tgagagaagc ttttctcttt tttcctaaat caagtgcgtc
    13681 acattgaaaa tcctcaaatt gttttataaa atgttttgac ttgtctaaaa aaatttttcc
    13741 ttcaggcgtt aattctattt tattacctat tctgtaaaaa agttcaaagc caagttcttt
    13801 ttctaattct ttaattgcta atgttatgga tggttgagaa atatgaatac tttccgcagc
    13861 cttagtcatg tttttatatt tacagacagt ttgaaaatag tataaatagt ttatattcat
    13921 ttggtattag ttcctttcat ttacatatta gtgacaagta tataattaac aatagcataa
    13981 gttttgtata ttgttgtaaa aaaatagaat agttttaaac tatcatagca taagtttttg
    14041 atattttcaa tctattaaga atagtgctat tattaaatta acaaaagaga atagctggta
    14101 tggtttagag taaatcaatc tatattacag ttatatcaac gaatatcaaa aaattatcta
    14161 ttagaaaata aaaattattt ttacaattaa attgttaatt aaatcataaa gaaaaaagtg
    14221 atattaacaa gataaaattt ttttaatcag aaatgttaat ttaataacaa aatagagtat
    14281 tggggagaaa ggagaaatat attttttaat aatatttgtt aatttaataa cgaaaaatga
    14341 aagggagtga tttagtgaag tttgaattga tggatttttt aatgaatcct tttgtcctta
    14401 tgtttgctgc agtaattaca ggaattttat ttggaaaaat taaatttggg aagtttaatt
    14461 ttggggtatc aggagcatta tttacaggat tatttattgg atggcttgca tatagcttgg
    14521 ggaatctaat aatagcaaaa ggagaaacag cagcaggata taaggcagca actgtaatga
    14581 tggggaatgg aattatttct tctgacttct ttgatttctt tttaataata tttgttgcag
    14641 cagttggtct attggcagca aaagatatga aagctgtatt aaagaagtat ggagcaagat
    14701 ttgtaatttt aggagttctt ataactttta taggtggctc tatgacttat gcaatgactt
    14761 tactatcaag tgataaagga agtagtgctt atgaagtttc tggcgtttac acaggagcat
    14821 taacaagttc gcctggatta gctgctgctt tagaaacagc tggtaaacat gcagaagatg
    14881 tatcaaaaga atttgaaaaa gcttcaataa aagataaaaa agaaatttta aaagtagtcg
    14941 acccagaagg caaattggat gtgaatacaa ctacatcact gactcaagag caaatagata
    15001 agtatattgc atatgctgaa gctggtgttg gtataggtca tgcagtcgca tatccttttg
    15061 gagttttaat agtaatctta ggtgtaaatt tccttccaaa attatttagg atggacctaa
    15121 aagaagaaag aagaaaatat gagaaggaaa tgaaggaagc aagagatagt gtttctggta
    15181 aaacgatacc agaagttcca tttaatataa taacattttt cttgacatgt ttagctggat
    15241 acttagttgg aggaatacat gtatttatgg gaccactagg gtacttcaca ctaggcgcaa
    15301 caggaggttc actaattgta tctctagttc ttggatatat aggtaagata ggggtagtaa
    15361 atttcagaat ggaagaaaag gttttaaata ttttaaaaca aatagggcta gtatttttct
    15421 tagcaatagt tggattaaga tatggtggaa aagtagttga ttcaattatg acatctggta
    15481 tgcacctagc cttagtagca atagcagtag gtgttacagc tatgatgata ggattcttag
    15541 ttggcaaata cgtatttaaa ttaaattgga ttttattatc tggagctgtc tgtggtggta
    15601 tgacatcaac accaggtctt ggagctgctg ttgatgctct tgatagtgat gacccagcag
    15661 caggttatgg tgctacgtat ccatttgcat tattaactaa agttatattg gttatagttt
    15721 tacacaaact tccaatgtaa ataagatttt atatgggggg atatttatga ataatgaagg
    15781 agcatttgaa gaattaagtg gaagattaag aaataaagaa ttgtgtgatt taataatgaa
    15841 tgcagatgaa gctgctaaaa ttatagagga tggcatggtt attggagtta gtggatttac
    15901 accttcaggc tatccaaaag cagtaccatt agcagtttca gaaagagcta aaagtggaga
    15961 aaagataaaa ttaaccgtat attcaggagc atctttaggc ccagaagttg atggagcatg
    16021 gtcagaagca ggtataattg aaagaagact tccttatcaa actaattcaa tacttagaaa
    16081 taatattaat aaaggtgttg ttgattatat agatatgcac ctaagtcatt ctactcaatt
    16141 tttaaattat ggaactatac ctaaagtaga tgtagctata gtagaagcac ttgcaataac
    16201 tgaagaaggg aatattatac caactagtgg gataggtaat tctccatctt ttataaaaag
    16261 tgcagataaa gtaatagtag agataaatct tgcaaaacca atggaaatgg aaggtatggc
    16321 agatatatat attacggaaa atccaccaaa tagaaaacca atagaaataa atcatcctaa
    16381 ggatagaatt ggaacaacat atataccttg tggtttggat aaaattgcag gagtagtaat
    16441 taccaatatg caagataaga cccgtccatt gggggttgta gatgaagctt cgaaaaaaat
    16501 atcaaataat attattgctt ttttaagaga agaagtaaag gcaggcagat tatcaaagaa
    16561 tttacttcct cttcaatctg gtgtaggaag tgtagcaaat gcagtattat atggattatg
    16621 tgaatctgaa tttgaaaatt taacttgtta tacagaggtt gtacaggact ctatgttgga
    16681 tttaataaga atgggtaaag ttacaatggc atctactaca tctgtaagtc catctccaga
    16741 aggattaata aaatttgaaa aagatattga tttttttaag gataagatta tattaagacc
    16801 tcaagaaatt agtaacaatc cagaaatagc cagaagaatt ggtgtaattg caatgaacac
    16861 tgcaattgaa gttgatattt atggaaatgt aaattctact cacataatgg ggtcaaagat
    16921 gatgaatgga attggaggtt ctggtgattt tgctagaaat ggagcaataa ctatatttag
    16981 tactgagtcg attgcaaaaa atggtgatat atcatcaata gtacctatgg tatctcacgt
    17041 agaccacact gaacatgatg taatggtaat tgtaacagaa caaggatatg ctgatttaag
    17101 aggtcttgca cctagagaaa gagctataaa aataatagaa aattgtgccc atccagatta
    17161 taaagaacaa cttagggatt atttaaatag agcttgtaaa agtggagcaa aacaaacacc
    17221 tcacatattg gacgaagctt tatcatggca tagtaaattt atgagtacag gtacaatgaa
    17281 aaaggctgaa acttttaaaa gtgcattata aaatatttaa taaattggag gtagatttta
    17341 aatggaaaaa gcagtggaaa actttgaaga tttaagtaag gaatacatta atggttatat
    17401 tgagagagca agaaaagctc aaagagagtt tgaatgttat actcaagaac aagtagataa
    17461 aatagtaaaa atagttggaa aagtagtgta ttataatgct gaatatttag ctaaacttgc
    17521 tgtagaagag actggaatgg gagtatatga agataaggta gctaaaaata aaagcaaagc
    17581 aaaagttata tataacaatt taaaagataa aaaatcagtt ggtataattg atatagatag
    17641 agaaactgga ataacaaagg tagctaaacc agttggagta gtagcggcaa taactccttg
    17701 tacaaatcca atcgttacac ctatgagcaa tgcaatgttt gcacttaagg gaagaaatgc
    17761 aattattatc acacctcatc ataaagcaat tggatgtagt acaaaaactg tagagatgat
    17821 taatgaagaa ttagaaaaaa taggagctcc agaaaattta atacaaatac ttgaccaaca
    17881 atcaagagaa aatacaagaa atttaatttc atcagcagat gtagtaattg ccactggagg
    17941 aatgggaatg gtaaaagctg cgtactcaag tggaaaacca gcacttggtg taggggcagg
    18001 aaatgttcaa tgtatcatag atagagatgt agacattaaa gaagcagtac ctaaaatcat
    18061 agcaggaaga atttttgata atggtatcat ttgttcaggt gaacagtcag taatagtagc
    18121 agaagaaatg tttgataaaa taatggatga atttaaaaat aacaaaggtt ttatagttag
    18181 agataaagta caaaaagaag ctttcagaaa tgctatgttt gtaaataaat ctatgaataa
    18241 agatgcagta ggtcaatcag ttcatacaat tgcaaaaatt gcaggtgttg agataccaga
    18301 agatacaaaa ataatagtaa tagaagctga tggaccagga gaagaggata ttattgcaaa
    18361 agaaaaaatg tgtccagtta tatctgcata caagtataaa agttttgaag aaggtgtagc
    18421 tattgcaaaa gcaaatttaa atgtagaagg aaaaggacat agtgtatcta tacattcaaa
    18481 tacagttaaa aatatagaat atgcaggaga aaatatagaa gtatcaagat ttgttattaa
    18541 tcaatgttgt gctactagtg ctggaggaag tttctttaat ggtcttgcac ctactaatac
    18601 tctaggttgt ggttcttggg gaaataacag catctctgaa aatcttgatt ataagcatct
    18661 aattaatatt tctagaattg cttattatat gccagaaaat gaagtcccta cagatgaaga
    18721 actttgggga taaaacttag acaaaaaata ttcattctaa attaaagtag gaatatattt
    18781 taatttagaa tgaatttaag acataaatta aatttaaatt agtttattgg actaaattta
    18841 aaatattaag agacatatag aagaaggaag tattataatt taaaataaat aaaaaaatct
    18901 ataaaagaga aggagtggtt ttattatggc attaatgaca ggagctcaat atattgaaag
    18961 tttgagaaaa ttaaatacta aggtttatat gtttggtgaa gaggttaaga attgggttga
    19021 ccatcctatg attagaccat ctataaattg tgtagcagca acttatgact tagctcatga
    19081 cccagaatat gcagatttaa tgacagttac ttcaaatatc acaggtgaaa aaataaatag
    19141 atttggtcat cttcatcaaa gtgttgatga tttgataaaa aaagtaaaaa tgcaaagact
    19201 atgtggtcaa aaaacagctt cttgtttcca aagatgtgtt ggtatggatg cgtttaatgc
    19261 agtgtattca acaacatttg agtgtgataa agcccatgga actaattatc atgataattt
    19321 tgtaaaatac ttaacttata tacaagaaaa cgatttagta gttgatggag ctatgactga
    19381 ccctaaggga gatagaagtc tatcacctag tgctcaacct gacccagata tgttccttca
    19441 tatagtagaa agaagagaag atgggataat tgtaagagga gcaaaggcac atcaaactgg
    19501 ttcaataaat tcacatgagc accttataat gcctactata agtatgactg aagcagataa
    19561 agactacgct gtatcatttg cagttccttc agatgctgaa ggtgtgttta tgatttatgg
    19621 aagacaatct tgtgacacca gaaaattaga agaaggtgca gatgttgatt taggaaataa
    19681 ggagtttgga ggtcaagaag ctttagtagt atttgataat gtatttgttc caaatgatag
    19741 aattttctta tgtggagaat gggatttctc tggtatgtta gtagagagat ttgcaggata
    19801 ccatagacaa agttatggtg gatgtaaagt aggagtaggt gatgtaataa taggagcagc
    19861 agctttagca gctgattata atggagcaaa caaagcttca catataaaag ataaactaat
    19921 agaaatgact caccttaatg aaagcttata ttgctgtggt atagcatgtt catcagaagg
    19981 tcacaaaaca gaagctggaa actatcaaat agatttactt cttgcaaatg tgtgtaaaca
    20041 aaatgtaact agatttcctt atgaaatagt aagacttgca gaagatatag ctggagggct
    20101 tatggttact atgccttcag aaaaagactt taaatcagac cttaaagttg gaacaagtgg
    20161 aatgactatt ggcgaagtat gtaacaagta ttttaaagct tcttcagtgg cttcaacaga
    20221 agaaagaatg agaatattaa gattcttaga aaatatatgt cttggttcat ctgctgttgg
    20281 ttatagaact gagtcaatgc atggggcagg ttcaccacaa gctcaaagaa taatgatttc
    20341 aagacaagga aacataaatc aaaagaaaga attagctaag aaaattgcgg gaataaaaaa
    20401 agaggaagct ttaaatttat aaaaagttga tatataaagg gaaattatat attaatgtgt
    20461 attaattaat gaaaagatat gtagtataag agatagttat aaaattatgg tggtttttag
    20521 ctaagtaaag aaatttttaa taaggagaat ttacacatga cctgtggagt tagattttgt
    20581 gggggctgta accctagata cgaaagaggg aaggctctta caataatcaa agagcacttc
    20641 aatggtaggg tagattttag catagcacaa gagaacgtcg agtatgattt gctacttata
    20701 attggaggat gcgcaactct atgcgcatcc tatcaacaat ataaagtgaa aaatgaatgt
    20761 gttaagatgt gggattttag ccatatagaa agagttataa atgtgattga atcagcaatt
    20821 tgtgaaactt aaaaaagcta aatgggggga tttcattgag ttggcaagaa ttgtatcaaa
    20881 gtaaattatg tagtgctaca gaagctgtaa aacagattaa aaacggtgat actgtagtat
    20941 ttgcacattg tgtaggtgaa cctcctgcac tagtagaggc tatgattgaa aatgctgaac
    21001 aatataaaga tgttgagatt aaacatatgg taagcttggg tagtggtgga tatactgcta
    21061 agggaatgga agctcatttt agagtaaatc caatgtttgt aagtggaaat gtaagaaagg
    21121 caattgaaaa tggagatggt gattttacac ctgcattctt ccatgaagta ccaaagttgc
    21181 ttagagaaaa aagattaaaa tgtgatgttg ttttagcaca ggtaactcca ccagatgaac
    21241 atgggtattg tagcttagga acatcagttg attatactta tgaagccatt aaaactgcta
    21301 gaacagtaat tgttcaagtg aatgaccagt ttcctagaac ctatggtgag gtagtccatg
    21361 tatcagagtt tgactatata gtggaaaaat cacaaccttt atttgaacta caacctgcaa
    21421 agattggaga agtagaagaa gcaattggta aaaattgtgc ttcattaatt gaagatggtt
    21481 ctactttaca gctaggtatt ggtgggatac cagatgcagt tatgttattt ttaactgata
    21541 aaaaggattt agggattcat tcagaaatga tttcagatgg gactttggcc ctttatgaaa
    21601 aaggtgttat aaatggtaaa tataaaaatt ttgataaaga aaaaatgaca gttaccttct
    21661 taatgggtac taaaaaatta tatgactttg caaataataa ccctgcagta gaggtaaaac
    21721 cagtagacta tgtgaatcat ccagcaatta taatgaaaca acataagatg gtttctataa
    21781 attctgctat acaagttgat ttaatgggac aggtagttgc agaggctatg ggacttagac
    21841 aatttagtgg tgttggaggt caagttgact ttatacgtgg agtgtctatg ggggaagatg
    21901 gaaaggcaat tatagctatg ccttcaataa ctacaaaaaa agatggtaca gtaatttcaa
    21961 aaatcgtatc tattgtggat gaaggtgcac ctataacgac atcaagaaat gatgttgact
    22021 atattgtaac agaatacggt atagcagaat taaaaggaaa atctcttaga gaaagagcaa
    22081 gaaatttaat aaatatagct catccaagtg taagagaaag tctagcagta gaatttgaaa
    22141 agagatttaa ggagaaatat taataataga ggggtgttaa taatggaagc acttagagta
    22201 gttccaaaga tattttattt tgatacattt aaagaattta atgaagaatt taaaatagga
    22261 aaaaatgatt tagtaattac taatgagttt atatatgagc catatatgaa acctcttgga
    22321 atagatacaa atttaatttt tcaagaaaag tttggaacag gtgaaccatc tgatgaaatg
    22381 atagactcca tgacaaagga aatgaagaaa tacaattttg atagaattat tgcgtttgga
    22441 ggaggaacaa tagttgatat atgtaagata ttagcacttg atgttccaga aaagtctatt
    22501 gatttatttg aaggagaagt atcacctaaa aaagtaaaag agttagtagt agttcctaca
    22561 acatgtggta caggtagtga agtaacaaat gtagctattg cagagttaaa atctaaacat
    22621 actaaaaagg ggcttgcagt agaagaaaca tatgcagact atgcagtgtt aattccagag
    22681 acagtaaaag ggttacctta caaattcttt gttacaagtt cggtagatgc tcttatacat
    22741 gctattgagt cttatctttc tccaaaagca agtccattta cagagatgta ttctctacaa
    22801 gcaataaaaa tgattatgga tgggtataag aagatagttg ataaaggaga agaagagaga
    22861 tttaatcatc ttagagactt tgtacttgca tcaaattatg caggaattgc ttttggaaat
    22921 gctggttgtg cagcagttca tgcactatct tattcaatag gtggagcttt ccatgttgca
    22981 catggtgaag caaattatca gttctttact gaagtattta agatgtattc tagaaaaaaa
    23041 cctaatggaa aaattataaa atgtacaaaa attttagcag atgcccttga atgtgaccct
    23101 aactgtgatg tttatggaga attagaaaag tttttaaata aattaatagc taaaaaagct
    23161 ttgagagaat atggaatggt ggaaagtcag atagatgaat ttacagatag tacaatagca
    23221 aatcaacaac gactacttgc taataattat gtagagctta gtagagagga aataagagag
    23281 atatttgcaa atctttatta atacaatttt ttatttatat gtatagtcaa cataaacaat
    23341 gctaattata tttatgtaca aataaaaata agggaactta aattttaatt aaagttaagt
    23401 tctcttattt ttatatggag ttaaatataa gtatattttt aatttaaaat atgcttatat
    23461 ttaagtctat tactttattt gaaacaattt gtagaatttc aaattccttg acagtaataa
    23521 gtaagtaatt gtataatcaa actgcaatat attaataata ttagtgaaaa tgaaaggatt
    23581 ataaaatatg tataaatcag aaaatttttc aaaaaaatct gtattaataa attcattttt
    23641 aagtaaattt ttaaaaataa ttggatgggg tttggtaagc ttatttacat tatcagcctt
    23701 agtactttca actatttcca ttataattgg atgggctgta atacttccta ttcaagtatg
    23761 tgcagtattt ttacttatag gaggagtttg tatttttaaa ggtaagagat taggagacca
    23821 aatttctcgt tataaaaaat attgtacaat tataaacaat agaaatatta ttccagtaga
    23881 acttattgca gaaaacacat ctaaatcatt aaattttata ataaaagatg tacaaaagat
    23941 gatagacaaa aactacttta taaatactta tattgataaa agaaataatc agattgtgtt
    24001 aacaaatgaa gattttattc caccaagata tgaagatgta gcatatgaag tcaaagaaga
    24061 agaaaagact gatgaatcca atgaagttga tacaattata aataaaggaa tgaattatct
    24121 aacacaaata aaagaagcta ataaaaatat taaatcagaa tcaatgggcg ataaaattgt
    24181 acaagtagaa gatgttacaa gtaaaatatt tgatgttgta aaacatgacc catcaaaact
    24241 tactcaaata caaaaattta tggattatta tcttccaacc actttaaaac tattaaattc
    24301 atatcataca ttggaagaac aaggaattga tagagaaaat ataacaacaa ctatggaaag
    24361 tattgaaaat acaatgaata cgattgtagt agcttttgaa aatcaattgg actatctgtt
    24421 tgaagatgaa gccattgata tttctacaga tattactgtt ttagagaata tgttagtcca
    24481 agaaggatta acgagtggta aattttaact tcaatagaaa aaatattctg ttatttttaa
    24541 acaattaatt agtcataaag tataaaatgt gtattatata agttaatcag tactttatga
    24601 aggaagagga gagaaagata caatgaataa tttagatgat ataccagtta tgccaacttt
    24661 gacacttgac ccatttggag aagaatcaaa cactaatgat atagataatt ctgacttatt
    24721 aatgaaaaag gatgaaaaag acccagaaga agaaaaactg tctgaatctg aacgcaagat
    24781 ggtaaaggaa tttgcggaaa aaatagatat tacaaataca aatatgattt tgcaatatgg
    24841 agctggtgct caaaaaaagg tagcaagttt ttctgaaaca gctttaaaga gtgtaaaaac
    24901 aagagattta ggagaagttg gagatatgct tacaaatcta gttactgatt taaaagcatt
    24961 ttcagcagat gaaaaagaac aaagtggttt tttaggaatt tttaaaaagg caaataataa
    25021 aatttcaaat ttaaaaacta agtatgatag tgcagaggta aatgtggata aagtaagtaa
    25081 agagcttcaa aagcatcagg ttaaaatatt gaaagatata gcaatgcttg ataagatgta
    25141 tgaattgaac ttagcttact caaaagagct tactatgtat attatagcag gaaaacaaaa
    25201 attaaaggat atgaaagaat atgaaatgcc aaagttgaga gaaaaagctc gtttatctgg
    25261 ttcaacagaa gatgctcaat ctgtaaatga tatggtttca ctttgtgaca gatttgaaaa
    25321 gaaaattcat gacttagaat taactagaat ggtatcgctt caaatggctc ctcagataag
    25381 attggttcaa aatagtaata atctgatggc agaaaagatt caatcaacta tagtaaatac
    25441 cattccatta tggaaaaatc aaatagtgct tgctcttgga atttctcact taaatcaagc
    25501 tatgaaagca cagcgtgaag ttagtaatat gacaaatgag cttttattga agaatgcaga
    25561 aactttgaag atgggaacaa tagaaacagc aaaagaatca gaaagaggaa ttgtagatat
    25621 agacacaatt aagaaaacta atcaatctct aatatctact attgatgagg tagttaaaat
    25681 acaacatgat ggtagaataa agagacaaga agcagaagtt gaattatcaa aaatagaaaa
    25741 tgaacttaaa agtaagcttc ttgaatttac agttaaataa aaattatact aatataaaaa
    25801 gagactgtct caaaatagag ataagtctct ttttcattgt ttaaatgatt aaagtatatt
    25861 tatattataa atttctatta tcttttaaca taacatcatg agctccacct tcaactatac
    25921 tagtagaaga tacaagtgta agtttagcat ttttttgaag ttcaggaatg ctaagtgctc
    25981 cacagttaca catagtagaa cgaactttac ttaaagaaag agttacattg tctttcaaag
    26041 aaccagcata aggcacataa gagtcaacac cttcttcaaa agaaagtttc ttatctccac
    26101 caaggtcata tctttgccag tttctagcac gagcagaacc ttctccccaa tactctttca
    26161 tataactacc atttatatta actttgttag ttggactctc atcaaaacga gagaagtatc
    26221 taccaagcat tataaaatct gcacccatag ctaaagctaa agttatatga tggtcataaa
    26281 cgataccacc atcagagcaa attggaatat aaatacctgt tttttcaaag tattcgtctc
    26341 tagcttgagc aacttcaatt atagaagtag cttgtccacg tcctatacct ttttgttcac
    26401 gagttataca tatagaacca ccaccaatac caactttaac gaagtcagca ccagcttcag
    26461 ctaggtaaag gaatccctcg cggtcaacta catttccagc tcctacctta actgtatcac
    26521 catatttttc acgaatgaaa tcaagtgtta ttttttgcca ctcagaaaaa ccttctgaag
    26581 aatctataca taaaacatca gcaccagctt caactaaagc aggaacacgt tcagcaaagt
    26641 ctctagtatt aataccagct ccaactacat atctcttaga agaatctaaa agttcaagtg
    26701 gattttcttt atgagaagag taatctttac ggaataccat atacataaga ttaccattat
    26761 catcaagtac aggaagagaa ttaagtttgt ggtcccatat aatgttgttt gcttccttta
    26821 aagtaacatc tttgttagca taaactatag atgacatagg agtcataaat tcactaactt
    26881 tagtatctaa atccatacga cttatacgat aatcacgact tgcaacaata cccaataatt
    26941 taccagttga agttccatct tcagtaacag ctactgtaga atgtccagtt ttttctttta
    27001 agtcaagtat gtcttttaat gtgttatcag gtttaatatt tgaatcactt acaacaaaac
    27061 cagctttgtg ggatttaaca cgtgctacca tagcagcttc attttcaata gtttgagaac
    27121 cgtatataaa tgaaatacca ccttctttag caagtgcaat agccattttg tcatcagaaa
    27181 cagattgcat aatagcagaa gtcaaaggta tattcatata aatatctgct tcctcgccct
    27241 ttttaaattt agtaacaggt gtttttaaac ttacattagc aggtatacaa tcagtagaag
    27301 agaaacctgg tacaagaagg tattcactga aagtatgtga tggtgtatca aaataaaacg
    27361 ccatttttta atcactcctt atatttttat actttttata attgatagta ttaattataa
    27421 cttattcttt aattttatta aagtattttg tcaaatattg agatttttga aaaatttaaa
    27481 ctattttaat taaatttgtt taatatatat tgccttatag ggtaaagttg tatattaaat
    27541 aatgtttagt gtttaatagt agctaattta aaattaatct ttagtgattc atataattac
    27601 atttgtatag tttatattga taatattact agagagtgca taaacctcaa aaatacagat
    27661 aaattaaatc tatattagta tatccaaaag tacatttaat aaatttattt aactgaatta
    27721 ttattttcat ttctattaaa tttaaatatt ttataaaaat agtattattt acaaaatcat
    27781 atactccaaa atactacata aataaaatta aacaaaaact aaaatgacaa atgtcattat
    27841 ctaaagtgac atagaaacgg tttcttaaag ttgttactat aaaatataaa ctctataata
    27901 aaagtataac aaaagggaat catatcgata tagaatataa ttcaagagga ggtaaaatta
    27961 tgagtagttc taatcaatcg acaataaaga catctataca gcgagtaggg aaatttttaa
    28021 gtggtatggt aatgccaaat ataggagctt ttatagcatg gggtcttata acagcattat
    28081 ttataccaac aggatggatg cctaatgaaa aattgagtac tataggcgac ccaatgataa
    28141 aatacctttt accactattg atagcttata ctggtggtaa agcaatagca ggacaaagag
    28201 gtggcgtaat aggtgcagca gcagctatgg gagtaattgt tggtgcagat ataccaatgt
    28261 ttatcggagc aatgataatg ggaccatttg caggatgggt aattaagaag tttgataaat
    28321 ttgtagatgg aaagatacca acaggatttg aaatgttagt aaataacttt tcaataggta
    28381 taataggtat gcttcttgca atactaggat tttatgcaat tggaccagca atagtagcag
    28441 ggacagctct tatagaatct ggagttcaat ttatagttag caaaagttta ttgcctctag
    28501 tttcagtatt tatagaacct ggaaaagtat tattcttaaa caatgcaata aatcatggta
    28561 tattagggcc tataggtata gctgaggcaa aagaagcagg taaatctata atgttcttac
    28621 tagaaagtaa ccctggacca ggtttaggag ttctattagc atattggatg tttagtagag
    28681 gaagtgtaaa acaatcagca ccaggagctg taataataca tttctttggt ggtatccatg
    28741 aaatttactt cccatatatt ttaatgaatc cagttttaat attggctaca atggcaggtg
    28801 gagcagcagg aatattgact ttttcaatat taggagctgg gcttgtagca gcaccatcac
    28861 caggaagtat atttgcactt atggcactag ctccaaaggg aggattactt ccagtattag
    28921 caggagttgc agtggcaaca gttgtatcat tcttagtggc agcaccattt gttaagagag
    28981 cttcagcaaa tcaaagtgaa gaagacagta cttctttaga agaagctaaa gcaaaaatga
    29041 gtgatatgaa atctgcaagt aagaactctg agaaaaatat tgaggaaaaa cagcttgaag
    29101 taaatgagat taagaagatt gtatttgcat gtgatgcagg aatgggttcg agtgcaatgg
    29161 gagcttccag atttaaaaat agaataaaaa acttagactt aaatatagaa attacaaatt
    29221 catctgttga taatcttcca gatgatacac aaatagtagt aactcacaat acattagttg
    29281 aaagagttgc taagaataat tcaagtgttg agatagtttc aataaacaat tttttaaatg
    29341 acccaaattt agatgcatta tttaaaagat tagaatctaa ataaaaataa aaagtaccac
    29401 taattaggag tggataattt gaaaaaaaag aagattacat ctaggcaaaa aaagataata
    29461 ctaatgatag tagaaaattc aaagaaaaac attccaataa ctatttctga gattgcaggg
    29521 actttagagc taagttcaag aactgtactt agagatatgt ctggaattga aaaatggttt
    29581 gatgaaaatg attttaactt tgtaaaaaaa ccaggagtgg gattgatttt agaggaaaat
    29641 atagaaaatc aaaactttat aattgagttg ttagaagaag aaaaaataga aaaagaatac
    29701 agcaaggaag aaagaaattt aattatatta tcaaagctac tagtttctaa cgagccagta
    29761 aaatcctatt attttgcaaa aatattaaag gtatctgaag gggtactgaa taatgatttt
    29821 gccttagcaa gtaaatggtt ggaaagattt gatattgagc ttgttagaaa accagggctt
    29881 ggtgtctact taaaaggtca agaaaaaaac tttagagagg catatgtaaa tcttatatac
    29941 gattcattca atgaaaaaga aatactagat atggtcagaa atataagtga aaatatacaa
    30001 acagataaag caatagaaat tttaagtgaa aatagacttt taaatttaat ggacaggtgc
    30061 ataataagaa aagttgaagc tacattaaca aagaagcttt cagatttaga tgtaaattta
    30121 gctgatagtg catatatagg gcttgtagtt catatttcat tagccttaca gaggataaaa
    30181 aatggtgaaa atataactat ggataaagag tttctaaaag aattatctat aacagaagag
    30241 tttaaattag ctaaagaaat cgtaaaaggt atggaaacag attttaacat ggatatacca
    30301 gtagatgagg ttggatatat aactatgcat ataagaggtg caaagcaaag gtcatcttca
    30361 aaccataagg ctttaaactt agatgatata gaaataatgg aaattacaaa taagatgata
    30421 gatttggcgg aagatgaatt taaaataagt ttaaaaaatg acgagaggtt atttaaagac
    30481 ctagcaaatc acttagggcc ttctataaat agattgaata tgggattaga aataagaaat
    30541 cctcttttag atgaaataaa aagcaagtat tcatatgcct atgatggagt tgaaaagatt
    30601 tctagaatta taaaagataa actaaatata aattcaatac ctgaatcaga aataggatat
    30661 attgcaatgc actttgcatc agccatagaa aaaaatctta tgatgaatac taatataaat
    30721 attgttgtgg catgtcccac tggaataggt acttcaagat tcttatcaac taagattgaa
    30781 aataaatttc ctaatttaaa tatacttgaa accatatcag ctataaacat agatgaagag
    30841 tatttaaaag aaaaagatgt tgatttaata gtgtctacag ttgagttaaa tacaagttta
    30901 aattatatat gtgtaggtcc atttatgagt ttagatgatg aacagataat caaggaaaaa
    30961 ataaaatcta tagctcaaaa taaattaatc aatttaaatg cgaaaaatga tactaaaagt
    31021 aagaataaag tatatgaaca gataacagaa agtatgaata taggaaaaga tattcttcaa
    31081 tttttagagg aaattagatt tgaaaaattt gaaagtaaag atttaagtga gcttattgaa
    31141 gactcatcaa aaatatttgc aaaatctagt gaagatataa tctctataaa agaatcgtta
    31201 aaagaaagat taaaaatctc aattccatat atagaagaat caaaaattct attgttacat
    31261 tgtatgagtg aaagaataga tattatgaag ttatcaataa taaaactaga gaatagaatc
    31321 gtgctagatt caaatgaaga aatcgataat gtagttttta tgttacttcc taaaaattca
    31381 ccaagttatc aaagacaaat aatgagtgaa ataagtggtt cattaataga caatataatt
    31441 tttactaata agataaataa attctctata gaagagatga cattagaggt aaaagatata
    31501 gtttttaatt tttatacaaa cagattaaaa gcatttatag ataaataaaa ttaatattaa
    31561 aaaggaaggt tgatactatg agtaaagtat tgaatgagaa taatatattt ttagggttag
    31621 atagtgtttc aaaggaagaa gctataactt tagcaggaag aaaattagtt gaaaatggat
    31681 atgtaaaaga agaatatata ccagcaatgt tagaaagaga aaaagtaatg actacatata
    31741 tgggaatggg agttgctatt ccacatggtg taaatgaagc aaaaaaagaa atattatctt
    31801 ctggaatagt tatattacaa ttccctaatg gtatagattt tgatggagaa aaagcatatt
    31861 tattaatagg aatagctggt gttggagatg aacatttaga aatactatct aatatagcag
    31921 tagttttaga tgatgactta acagaaagac taaaaaattc aaatgacaaa caagctttta
    31981 tggaagcttt tgccaactaa tcttaggagg gatttaaaat gaaaaaggcg attcagtttg
    32041 gagcaggaaa tattggaaga ggttttattg gaagcttgtt agttaagtcg ggttatcatg
    32101 tagtatttgc tgatgtaaat gaggagatat taaactcaat aaacaaagat aaaaagtata
    32161 ctatacacat aagagatgta gaatgtatag atgaagtaat tgataatata agtgcggttt
    32221 catctataaa agaagaaata atagatgaga ttgtacaggc tgaaataata actacagcag
    32281 ttggaccttt agttttgact aaaatagcct ctacaattgc aaaaggtata aaagctagaa
    32341 aagaaaaggg attaacaagt aatctaaata taatagcatg tgaaaatgca atatacgcaa
    32401 gttcttcttt aaaagaagag gtacttaagt atttaaataa ggaagaagta gaatatttag
    32461 agatgtatgt tggttttcca aactgttcag ttgatagaat agttcctcct ggtaaaaacg
    32521 aaaatcctct tgatgtaaca gttgaaaatt tttatgagtg gaatgttgaa aaacaaggat
    32581 ttaaagggga aataccaact atagttggaa tgaatttagc tgataacctt atggcttata
    32641 tagaacgtaa attgtttaca ctgaatacag gtcatgctat aacagcttat ataggatatt
    32701 taaaaggata taagactata gaagagagta taaaagataa atttatctgt gatatagtca
    32761 aaagtgcaat ggtagaaagt ggagaaggat tgattaaaaa atacaatttt gactcagaag
    32821 tgcactataa atatattgat aaaattttaa atagatttaa aaatccatat cttaatgatg
    32881 atgtattaag agtcggaagg gaaccattaa gaaaactaag tgataaagat agattgatta
    32941 aacctttaat gacagcaaaa tcatatggat tatcagttga caatttgatt cttggaatag
    33001 gtgcagcgct tcattataat aatagtgaag atactcaaag tgtagaatta caagagttga
    33061 taaagtctat tggggtaaaa aaagccgttg ctaaaatagc taatataagc aatgacgaag
    33121 aaattttaaa taatatcgaa aaaagttata tatttatgaa aaatttataa atttacttga
    33181 aaaaaagtag ttctgataaa gtaactataa tatattattc tacacagttt gaattatttg
    33241 ttgaataata gagaattata atatataatt aaaaggaata aattaatata tttacctgta
    33301 tatgtttgat aatatggttc aaatgtttct actaagctac cattaatagc ttgactacag
    33361 gagtgaagaa gatatagttt atacttattt taaattatat attaagttaa ttactttgaa
    33421 ttttaagctc ctgtctatga tatagacagg agctttttta gtattttaat ataaaatact
    33481 tacaagaata gatagcaaaa ttagaacaaa tataattttt ttattaatat aattttagaa
    33541 agggctaggt ggaagttcaa tgaaggcttt acaagaaaag atattgagag aaggcagtgt
    33601 ttcaggaaat gatatactaa aagttgatag ttttttaaac catcaaattg atgtagcttt
    33661 tttaaatgaa ataggaagag aatttaaaga gagatttaaa ggagaaaagg tggataagat
    33721 atttacaata gaggcttcag gaatagctat agcatcaatt gtttcacagt attttgataa
    33781 tgcacctgta gtatttgcaa aaaaatcaga atctaaaaat ttggataaag atgtatatga
    33841 aactaatgta tattcattta ctaaggcaag agagtattca gttaaagttt ctaaaaagta
    33901 cataaacaaa ggcgaaaata tattgatagt ggatgatttt ttggcaaatg gaagagctgc
    33961 tcttggactt aaagatttaa ttgagcaagc agaagctaac ttagttggag taggaattgt
    34021 cattgaaaaa ggatttcaag ctggaggagc acttttaaag gctaatgatg taagattaga
    34081 gtctttggca gtagttgaat ctatagataa tggaactgta aaatttaggt aatttatgat
    34141 attatataat tgtgtttagc acatatttta actatgtaat tatggaggat tttttatgag
    34201 gttttttata gacacagcca atattgaaga aataaaagaa gcaaatgatt taggtgtaat
    34261 atgtggtgta actactaatc cgtctctaat agctaaagaa ggtagagatt ttatagaggt
    34321 agttaaagaa ataagtgaaa tagtagatgg gccaataagc gcagaagtaa taagtttaga
    34381 acataaaggt atgatagagg aagcagaaaa attatctaag atacataaaa atatagttat
    34441 aaaaatacct atgacagcag aaggtctaaa agcggtgaaa gtactatcaa gtaaaggtat
    34501 aaagactaat gtaacgctta tattttctgc tggacaagct cttttagctg ctagagctgg
    34561 agctacatat gtaagtcctt ttgttggtag attggatgat atatcacaaa atggtcttga
    34621 tttaattgaa gaaatcgttg atatatttag tgtaaatagt attgaggctc aaataatagt
    34681 tgctagtgtt agaaatccaa tacatgtttt acaagctgct agaatgggag cagatatagc
    34741 aacagttcct cttaaagtta taaaccaaat gattaagcat cctttgacag acaaaggaat
    34801 agatagcttt atgaaggatt gggaaggtgc ttttttaaaa ttataattat aaactacgct
    34861 ttataagtat attaaagagg ttattatgga aattctaagt aataaggcta ggctattttg
    34921 cctagtctta ttttggtgta taatatattg aaacatatta ttgaaataga ttaatttttt
    34981 aagtgaggag gcgaaaaatg gataaaatat taagagagat acaaagagaa gataaaaaaa
    35041 atccatatac tgaccaagaa cttgctgaaa tgttaaatgt agctaggtct gaagtgatat
    35101 cagttaggaa aaaaaataat attttagatt cgagagaaag aagaaaaaaa atattaataa
    35161 aagatatatc aaaaattgta agtgaaaatc ctcaaatgtc tgaaagaaaa ataactgaaa
    35221 tattaataga aaatggatat aatatatcga gaagtgcagt ttcaaaatta ttaaaagagg
    35281 aaaatttaaa tcagtcaaag attgggatta aggataaaga cccagcatca tcaggaaagt
    35341 atgtgaaggt gagtaaagtt gtagaagaag atggatttga agaattgata ggtataaagg
    35401 gaagtttgaa agaaaaggta aatctagcta agtcagcaat aatgtatcct cctaatggcc
    35461 ttcatactat aatatatgga gaaacaggag taggtaaaag tgaattagct acatgtatgt
    35521 ataaatatgc tataaaaaac aacattaaag aagagaacag cccatttata gtgtttaact
    35581 gtgcagacta tgcagaaaat cctaatctat tgatagctca actctttggg gtagtaaaag
    35641 gtgcatatac aggagcagat gcaaatagag aaggtcttgt agaacaagct aataatggaa
    35701 tattgttttt agatgaaatt catagattgc ctgctgctgg gcaagaaatc ttattttcat
    35761 taatagatag aggagaattt agaaggttag gagaaagtag ttctaataga aaggcaaatg
    35821 ttctcataat aagtgctaca acagaaaatc cagattctaa tttacttcaa acatttagaa
    35881 gacgtatacc aatggtaata aatttaccta atatgagtga aagaccaaaa tctgaaagat
    35941 atgaaataat aataaagttt tttgaaagag aagcaaagcg agtaaacaag aattttataa
    36001 taactaaaga agttatgtgt gcacttatga attataagtg tactggaaat ataggtcagt
    36061 taaagagtga tatacaagta acttgtgcta gagcttttag taaatcgata ttttctagcg
    36121 ataaggtaat tatagattta gatagtttaa gggattatat aaaaagtgga tattatgatt
    36181 gtaattgtaa aaatgaaaag tattctgagt tttcagttga ggatatatat atagatatta
    36241 gtgaattggt gagtggaaaa aatagaattt tatctgataa tgagataagc gagatatata
    36301 attatgcaga aaaagagctt aaggttttgg aaagtaagtg cttttcagaa gaagagttaa
    36361 aaaatgcatt tatagaaaag ttagataaga agtttaatgc tattaaaaac aataaaaatt
    36421 tatctgatag aagaagtcga atagactggg gaggacaatt aaaagagtct acactggaga
    36481 taatggataa ggtaattgta tttattaagg agcagtttaa gcatgtaaat caaggtctgt
    36541 acttggctct agcaattcat attgagcatg ctataggtag gataaaagat ggaaaaacta
    36601 taataaatcc gagtttagat aaaataaaag catcaatgcc aatggaatat gagttgtcaa
    36661 gatatataat gggtatagta gaagatttga cacaagtaac atttccagaa gatgagcttg
    36721 gatatttggc ttattatata aataaatttt gttataatga ggaaagtata aaagataaag
    36781 ttaaagtagt aatagttacg catggtaaag ttggaattga aatgtctaaa gttgtaaatc
    36841 atattttagg tatagaatgt acactaggta tagaaatagc acttacagat tcaccatcag
    36901 aggggataga gcatgtatta gaagagcttc aaaagataga agctagaaaa ggtattctag
    36961 ttctaattga tatgggttct ttggtaattc ttggtgatga ggttgaaaaa aggcttggaa
    37021 ttagatgtaa aactgtaaat agagtagata cattattggc aatggaagca ggaaaacttg
    37081 caaccattga aggaaaaagt ttagatggta taatagctga tttaaagaaa aataagaatt
    37141 atgctatgat aaatacaaat aaatttagtt acagaaaaaa cgagtatgga aagaaaaatg
    37201 taatcattac tttatgtttg agtggtgtag gtacagcttt aaatttaaaa gagcatatag
    37261 aaaaacaaat taaggaatat ggcactttaa ttgaagtaaa gccagttgct tttttaaata
    37321 ataatttaga agaagagtta aatgatattg aagctgaata taatattatt gctatctgtg
    37381 gaactattga tatagattat aaaaacgtgc cttttatttc ttataatgag gtacttggta
    37441 aaaatggtat taataaagta ttagagcatc taaatagtaa acaaccagct ttaaatattg
    37501 aaaaccagtt gaataattta atacatgaag atttgatttt atacaatttt gaaggaattt
    37561 caaaggagta tataattgat actcttgttt caaaacttga agaaggtgga tatgttgatt
    37621 caaagtatat attaagtgta tataaaagag aagcaatggg aagtgttgta atggcctcta
    37681 aggtagccgt gccacatgga cttccagaaa acgttataaa gcctgcaata gcaatcgcaa
    37741 ggttgaataa acctatagta tgggacaata aatttatggt ggatttggtg gtactactgg
    37801 cacttaaaga aaataataag aaagaaatac gaagcttatt ttcaaagatt aatgaccagc
    37861 atactttaga agttttatta aatactgaag ataaaaatga aataaaagaa cttttaagct
    37921 aattattagt accgagaaga aagacaaaaa aatttgcttt agtataaaat ttaaaattac
    37981 tcaattgcta agtagttaat tactctatat aaggctagtt ctatgaaatt tatttatatg
    38041 attttataga attagctttt ttcttggtaa ataaaatata tgaatttatt tatgtaaaaa
    38101 gtaaatttta ttggacaatt ctatgaaaat ttttcctata tttcttataa aatatagaat
    38161 atataaaaaa tatataaaaa taaattaaat tcataataaa taattttata aaatagttgt
    38221 taaacataaa aaaccatttg taaaaaacaa aaaaatcatt tctctaacaa aaaaaaccac
    38281 aaaaaatata aaaaagtatg gctagttaag tgattaacaa gtaaaaatta aaataaaaat
    38341 caaaaaaaat ttatcaatga aagtcagcta aaatcctgga agagagcatt attttcggga
    38401 aaacattttc ttttgtttca caaaaaaagc cactttttgg cacgcaacat gctttatata
    38461 aagtcaagaa aaactaaggt ataagacaaa ggcaattagg aggtgtaaat atggagttta
    38521 taaattttgt aagtgataaa gttatagacc ttaaaatgga ttgtaaaacg aaagaagatt
    38581 tctttaaaga aatacataat aaagtttttg atttaggctt tgtaaaagaa gaatttggag
    38641 aaaaaatatt agcaagagaa aatgtatttc caacaggttt aaatttaggt gattatggag
    38701 tagcaatacc acatacagat gcagaatata ttaaagagca atttatatca gtctgtactt
    38761 ttaatgaacc agtagtattt agttctatgg aagaccaaga tgaaaaagta cctgtaaatt
    38821 tggctttcat attaggactt aatcaaccac acagtcaatt atctgtatta acagaattaa
    38881 tgggaattat gcaaaataaa gagttagttg ataagttgat gagttctact gataaagaag
    38941 aagttttaag gacactaaag tctttataaa aataaaaaag tactaaaaat aaaaaatata
    39001 aaaataaaaa ttattaggag gaaataaaaa tgaaaaaaat attagtagca tgtggagcag
    39061 gaatagcaac atcaacagta gtttgtgaca aggtagagag attagttaag gaaaataata
    39121 tacaagcaga agttatacaa tgcaaaatag ctgaatgtgc aacaaagcaa gaaggagcag
    39181 atttaatagt atcaacaact atactaccaa caacttataa tataccagca ataaaagcaa
    39241 cagcatatat aactggagta aatacagcag cattagataa aaaaatatta gatgctttaa
    39301 aataatccta tcatagatac attgaaataa tattttataa aacagaaaaa ggaggattta
    39361 taaatggata aattattagc agtagtacaa tacattttaa atatgggacc gacagtaata
    39421 ctaccaataa caattgtagt actaggaatg atatttagaa ttaagtttac aaaagcttta
    39481 aagtcaggtc ttacaattgg tataggattt gtgggtataa acttagttgt aaacctatta
    39541 acatcaacac tagggccagc ggctcaagga atggttgaaa gattcggact taatttaaca
    39601 gtaatagatg ttggttggcc aacaacagca tcagcaacat gggcttcacc agttgcacca
    39661 atattaatac cagtttgttt attagtaaac ttactattat tatacttcaa taaaacaaag
    39721 atattaaacg ttgatatatg gaattattgg cactttatag tagcaggtgc agttggatat
    39781 atagtaacag gaagttatgt ttgggctata ttctgtgcaa tactaatgga gttattagta
    39841 ctgtttattg cagaaaagac agcaaaaaat gttcaggagt tttatgattt agaaggtata
    39901 gcattaccaa ctggttcaac agcttcattt gctccacttg gatatttagt aggtaaattt
    39961 gttgaaaaaa taccaggaat aaataaaata caagcagacc cagactctat acaaaaaaga
    40021 tttggtatat ttggagaacc aatgatgatg gggattatac taggtataat acttggagca
    40081 cttgcgggat atgatgtagc aggaatattc aatgtaggta tatcaatggg tgcagtaatg
    40141 ttacttatgc ctagaatggt tagaatatta atggaaggtt taattccaat ttcagaatca
    40201 ataagagatt tcttacaagc aaaatatgga gacagagaac tttatatagg tctggatgca
    40261 gcagtagcaa cgggacatcc agcagttata tcaacagctc ttatacttgt tcctataacg
    40321 ttgttcttag cagttatact accaggaaac aaagttttac catttggaga cttagcgaca
    40381 ataccatttt atatggcatt tatagtgtgc tttagaaaag gtaatatagt tcaatcagtt
    40441 ataacaggaa caatagtaat agctatatca ctttatatgg ctacaaactt tgcagatgtt
    40501 catacacaat tattacaaca agcaaatatg atgccagaag gaacaacaat ggttacaagt
    40561 atagatacag gtggaaactt gctaaaatgg ggaatattga aattatctca attagtttct
    40621 tcatttggaa tattctaata tgtagttaat agagattact cttttttagt aaagtatgcg
    40681 aaaatttaac atttagcagc tactaaaaaa gagtaatatt ttttataaat ttttaaaaat
    40741 acaaaataca agaggaggag atttatcgtg aaagcagcag tattacatgg cacaaatgat
    40801 atgagatttg aagatataga aataaaacca tgtgaaagtg atgaagtaaa aataaaagtt
    40861 atggcagcag gaatatgtgg gtcagatcct ccaagagtat taaaacattg gaaatatcca
    40921 gttccagcta taccaggaca tgagttttct ggagtaattg ctgaagttgg taaggatgta
    40981 aaaaatgtaa aagttggaga tagagtagtt gcaattcctt ttataccttg taatgaatgt
    41041 gaatattgta aaagaggatt attttcattg tgtgatgacc atggaatgtt aggagctaaa
    41101 agttttggag cttttgcaga gtatgtaaat ataaaagcta caaatgtact tccaatagga
    41161 gatatggact ttgaagatgc tgcaatgata gagccactag cagttgctat gcatggagta
    41221 cttaatatag gtgttcaagt tggagacgct gtagccgtaa tgggtagtgg aacaatgggg
    41281 cagttggtaa tacaaggact taaaatagca ggagcaggaa caattatagc agttgatata
    41341 tctgataata aattaagaga atcaaaggaa ttaggggctg atataataat caatgctaaa
    41401 gatattgacc cagtagaaaa aataaaagaa cttactggtg gaaaaggcgt tgacatagct
    41461 cttgaatgtg caggttcaaa aataactcaa gaacaatgtt tacttataac taaaaagaaa
    41521 agtaagatag gatttttagg gatagcatat tcagatataa ctttatcaga agaagcattt
    41581 gaaaatatat ttagaaaaga actagaatta aaaggttttt ggaactctta ttctgcacca
    41641 tttccaggac aagaatggac taagggaatc aatctagtta atgaaggaaa gataaaatta
    41701 aaagaaatgg tatctcatag attctcactt gaagatactt acaaagcatt tgaaatgata
    41761 agagatagaa aagaagaatt taacaaaata ttaattttac cacaaggggt tgaaaaataa
    41821 tgaaagcagt agttaaaact aaaccaggct atgataatgt agaagtttta gaagtagaag
    41881 aaccaaaagc tacaggagac aaggttaaaa taaaagtaga atatagtggt atatgtggtt
    41941 cagatataca ttcatttaaa ggtgaatatg caaatataaa agccccagta acattaggtc
    42001 atgaattttc tggaattgtt gtagaagtag gagaagatgt taaagatata aaagtaggag
    42061 atagagttac atcagaaact acttttgcaa cttgtgagaa atgtatatat tgtgaaacta
    42121 aagattataa cttatgccca actagaaaag gtattggtac tcaagtaaat ggaagttttg
    42181 ctgagtatgt actatcaaga gaagaaagca tacatgtact tcctgaaaat gtatctttat
    42241 tatcagcagc ccttacagag ccactagctt gttgtgttca tgcagctctt gaaaagacta
    42301 ctatagaaag ttctgataca gttttaataa taggaccagg gccaattggt ttattattag
    42361 ctcaagttgt taaatctcaa ggtgctacag ttataatgtc tggtgttaca aaagataagg
    42421 aaagattaga aatagctaaa aatcttggag tagatagaac tgtaaatgta atggaagaaa
    42481 gtttggcaga tgtagtgaat gaaatgactg atggaaatgg agtaaacaaa ggatttgatt
    42541 gttctgggtt tatgccagca gttaacgaag ccttaagact gcttagaaaa aaaggtactt
    42601 tcgtacaagt tggaatcttt gctaaaaaat taaatgaaat ggaccaagaa gcaataatcc
    42661 aaagagagtt acaatatata ggaagtagat ctcaaaagcc aagttcttgg attacagcat
    42721 tagatttaat ggcaagtggg aaagtaaata cagaagcttt agttactaga actgttggtt
    42781 tagacgaatt taaagaaggt attacagctt taatgaacgg ggaagaaata aaagtagcag
    42841 taaaatctta aaaaaacatt tgtattattt taaaaaaaat tgtaaaatct actgtaggat
    42901 aaaaaaatgt aaggtcttaa aaaataacag gaggaagaaa gtatgagcac tcttagagat
    42961 cattctaata atattagaaa aaacataata aaaatggttg cagaagctaa aagcggacat
    43021 ccaggtggtt cattatcaat agctgatatg ttgacagtat tatattttga taagatgaac
    43081 gtatgtgcag aaaatccaaa aatggctgat agagatagat ttgttctatc aaaaggtcat
    43141 gcagcaccag ctttatatgc tacattagca gaaaaaggat ttttcccaga agaagaatta
    43201 ataacactaa gaaaatttgg ttctaagtta caaggtcacc cagacatgaa aaaagtagca
    43261 ggaatcgata tgtctactgg ttctcttggt caagggctat ctgcagcaaa tggtatggca
    43321 cttgcaggaa aattagataa caaagactat acagtatata ctatattagg tgatggagaa
    43381 atccaagaag gtcaaatatg ggaagctgca atgacagcat cacattacaa attagataat
    43441 ttaattgcat ttgttgactt aaatggatta caaatagatg gttctaatga agaagtaatg
    43501 aatgtaaatc cagtagatga caaattcaaa tcttttggat ggaatgtaat agtaataaat
    43561 ggacattctt ttgaagaaat aggaaatgca gttgatgaag ctaaaaaagt tacaggaaaa
    43621 ccaactgtaa taatagctaa aacagtaaaa ggtaaaggtg tttcatttat ggaaaataat
    43681 gctgcatggc atggaacagc tccaaatgca gaacaagcag agcaagcatt gaaagattta
    43741 gaaggaggtc tataggaatg ggaatagcaa ctagagaagc ttacggacaa gtgttaaaag
    43801 aacttgcaga aaataaagat atagtagtat tagatgcaga tttaggaaaa gctacaaaaa
    43861 gtatatcatt taaagaagtt gccccagata gattttttga catggggata gcagaaggtg
    43921 acatgattgg tacagcagca ggtcttgcta cttgtggaaa aatacctttt gcgagtactt
    43981 ttgcaatatt tgcagcagga agaggctatg agcaaataag aaactctgta gcatatccaa
    44041 atttaaatgt aaaaatagca gctactcatg caggtgtaac agtaggtgaa gatggtggta
    44101 gccatcaagc tatagaggat atatctctaa tgagaggtat accaaatatg gttgtattaa
    44161 atccagcaga tgctttagaa gcaagacaag ctattcttgc atctattgat tataatggac
    44221 ctgtttatat aagattaggt agagctgcaa ctcctgatgt aaacagtgaa aattataaat
    44281 ttgaaatagg aaaaggaact gttttaagag aaggttctga cataacagta atagctacag
    44341 gaataatggt agcaaaagcc ttagaagcag cagaagaact tgctaaagaa ggtataaatg
    44401 tagaagttgt taatatatct acaataaaac cattagatga agcattaata aaagaaactg
    44461 ctaagaaaac tggaaaagta gttacagcag aggagcacag tataataggt ggtttaggtt
    44521 cagctgtatg tgaagcttta gcagaaacta aagatgtagt agttagaaga attggtgtta
    44581 aagatgtatt tggtcaatca ggtacaccag ctgatttatt aaaacactat ggtttaacta
    44641 cagaagatat agtaaaaaat ataaaagagt tgttatagat ataaatatat ctaaataaca
    44701 aattgatttg acataagtca attaaagctg tcttaaaaat agaaagaaat ttctacaatg
    44761 ggacagcttt atttttacaa tactttattt ttatgataat tataaaatag tctgaaagta
    44821 atcaaaaaaa ttataaaaag aggtgctatt taatgaaaat atctattgga tgtgaccatg
    44881 gtggttatga attaaaggaa tatataaagg aaaatttaat aaataaaggt atagaagttg
    44941 aagatgtggg tacaaattca aaagaaagtg tagattttcc aatatatgca aaaaaggtaa
    45001 gtcaaaatgt tcaaagtaaa aaatctgatt taggcatact ttgttgtgga acaggaatag
    45061 gcatgtctat tgcagcaaat aagtttaaag gaatcagagc agctgttgtt tctgactgtt
    45121 tttcagctaa ggcaactaga gaacacaata atacaaatgt actttgttta ggagaaagag
    45181 ttattgggaa aggattagca atgagaattg ttgaagaatg gttaaattca agttataatg
    45241 gagaaagaca tgcaagaaga ttacaaatga ttgatgatat agaaaaagaa aatttataaa
    45301 tatatataat atataattta taacaagggg gagcgttatg tctattattt gtgcttcaat
    45361 aatgtgtgca gaccaaatga agttaaaaga ggaattagaa gctcttgaag aagcaggtgt
    45421 aaaattactt cattgtgatg ttatggatgg aatatttgtt aaaaacttgg ctatgggacc
    45481 agagttatta aaatcaataa gtgaaaatac aacaatacct ttagacatac acttagccac
    45541 agaaactcca gataaatata ttgatatgat gtcttatata aagccaaaat atatttcttt
    45601 tcatgtagaa tcaagtacaa atgtaaaagc tgatatacaa aagttaagaa attatggtat
    45661 agggccagtg ttagcaataa gccctcaaac aagtgtggat aaaatagaag aatatataag
    45721 tttagttgag ggtatcttag taatgacagt aaatccaggg tttgcaggtc aaaagtttaa
    45781 tctttctgta ttggataagt tggataaact tacagagata ctaaaagatt atgataatcc
    45841 tccatttata gaggttgatg gaaatataaa taaggatact attaaactta tgaatggtaa
    45901 aaaagtagat atatatgttg ttggtacttc agctttattt aatgataagc ctccaatttc
    45961 atataaagat aaaatagaag aactaaaaga aagtataaag taaccatg
//
LOCUS       DAEPOG010000010        43740 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEPOG010000010 DAEPOG010000000
VERSION     DAEPOG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.010"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(90..548)
                     /locus_tag="KR432_000489"
     CDS             complement(90..548)
                     /locus_tag="KR432_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0639044.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(830..1117)
                     /locus_tag="KR432_000490"
     CDS             complement(830..1117)
                     /locus_tag="KR432_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBH0639045.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(1195..1620)
                     /gene="ruvX"
                     /locus_tag="KR432_000491"
     CDS             complement(1195..1620)
                     /gene="ruvX"
                     /locus_tag="KR432_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH0639046.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(1826..3997)
                     /locus_tag="KR432_000492"
     CDS             complement(1826..3997)
                     /locus_tag="KR432_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639047.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            complement(4007..4264)
                     /locus_tag="KR432_000493"
     CDS             complement(4007..4264)
                     /locus_tag="KR432_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBH0639048.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(4289..6928)
                     /gene="alaS"
                     /locus_tag="KR432_000494"
     CDS             complement(4289..6928)
                     /gene="alaS"
                     /locus_tag="KR432_000494"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH0639049.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(6996..7234)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7377..8456)
                     /gene="mnmA"
                     /locus_tag="KR432_000495"
     CDS             complement(7377..8456)
                     /gene="mnmA"
                     /locus_tag="KR432_000495"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH0639050.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(8745..9185)
                     /gene="nifU"
                     /locus_tag="KR432_000496"
     CDS             complement(8745..9185)
                     /gene="nifU"
                     /locus_tag="KR432_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBH0639051.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(9239..10432)
                     /gene="nifS"
                     /locus_tag="KR432_000497"
     CDS             complement(9239..10432)
                     /gene="nifS"
                     /locus_tag="KR432_000497"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBH0639052.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(10450..10896)
                     /locus_tag="KR432_000498"
     CDS             complement(10450..10896)
                     /locus_tag="KR432_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0639053.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(11005..11775)
                     /locus_tag="KR432_000499"
     CDS             complement(11005..11775)
                     /locus_tag="KR432_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0639054.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(11806..13071)
                     /locus_tag="KR432_000500"
     CDS             complement(11806..13071)
                     /locus_tag="KR432_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBH0639055.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(13178..13963)
                     /gene="codY"
                     /locus_tag="KR432_000501"
     CDS             complement(13178..13963)
                     /gene="codY"
                     /locus_tag="KR432_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBH0639056.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(14128..16215)
                     /gene="topA"
                     /locus_tag="KR432_000502"
     CDS             complement(14128..16215)
                     /gene="topA"
                     /locus_tag="KR432_000502"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH0639057.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(16248..17348)
                     /gene="dprA"
                     /locus_tag="KR432_000503"
     CDS             complement(16248..17348)
                     /gene="dprA"
                     /locus_tag="KR432_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH0639058.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(17367..18968)
                     /locus_tag="KR432_000504"
     CDS             complement(17367..18968)
                     /locus_tag="KR432_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH0639059.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(18999..19343)
                     /locus_tag="KR432_000505"
     CDS             complement(18999..19343)
                     /locus_tag="KR432_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH0639060.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
     gene            19528..20688
                     /locus_tag="KR432_000506"
     CDS             19528..20688
                     /locus_tag="KR432_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0639061.1"
                     /translation="MNRKKTFIFIRYLSLFILLISYLNHTENTLYPAITMIILFLIIN
                     NQVRFFSLSSENKFILISYFLELILIFIFCKYIKTFNPLYYVSLILDASFLIKEKYKY
                     ILFSLVIIFSFIIGLDKNLYLALESSSTLLIITLFSVYLENENLSKLYNQSLYDKLRV
                     SKDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLK
                     DKPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSFIKLTNIN
                     VRLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNLIITISDN
                     GIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFFE"
     gene            20681..21325
                     /locus_tag="KR432_000507"
     CDS             20681..21325
                     /locus_tag="KR432_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639062.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            21513..22448
                     /locus_tag="KR432_000508"
     CDS             21513..22448
                     /locus_tag="KR432_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639063.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            22462..23559
                     /locus_tag="KR432_000509"
     CDS             22462..23559
                     /locus_tag="KR432_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639064.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            23562..24626
                     /locus_tag="KR432_000510"
     CDS             23562..24626
                     /locus_tag="KR432_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639065.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            24867..25364
                     /locus_tag="KR432_000511"
     CDS             24867..25364
                     /locus_tag="KR432_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0639066.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(25541..25789)
                     /locus_tag="KR432_000512"
     CDS             complement(25541..25789)
                     /locus_tag="KR432_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639067.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(25855..26871)
                     /locus_tag="KR432_000513"
     CDS             complement(25855..26871)
                     /locus_tag="KR432_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0639068.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(26929..27696)
                     /locus_tag="KR432_000514"
     CDS             complement(26929..27696)
                     /locus_tag="KR432_000514"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH0639069.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(27764..29953)
                     /locus_tag="KR432_000515"
     CDS             complement(27764..29953)
                     /locus_tag="KR432_000515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBH0639070.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            complement(30518..31810)
                     /gene="brnQ"
                     /locus_tag="KR432_000516"
     CDS             complement(30518..31810)
                     /gene="brnQ"
                     /locus_tag="KR432_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0639071.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
     gene            complement(32928..34220)
                     /gene="brnQ"
                     /locus_tag="KR432_000517"
     CDS             complement(32928..34220)
                     /gene="brnQ"
                     /locus_tag="KR432_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0639072.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
     gene            complement(34609..35505)
                     /gene="ylqF"
                     /locus_tag="KR432_000518"
     CDS             complement(34609..35505)
                     /gene="ylqF"
                     /locus_tag="KR432_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBH0639073.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            complement(35826..36176)
                     /gene="rplS"
                     /locus_tag="KR432_000519"
     CDS             complement(35826..36176)
                     /gene="rplS"
                     /locus_tag="KR432_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH0639074.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            complement(36361..37056)
                     /gene="trmD"
                     /locus_tag="KR432_000520"
     CDS             complement(36361..37056)
                     /gene="trmD"
                     /locus_tag="KR432_000520"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH0639075.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            complement(37065..37580)
                     /gene="rimM"
                     /locus_tag="KR432_000521"
     CDS             complement(37065..37580)
                     /gene="rimM"
                     /locus_tag="KR432_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBH0639076.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            complement(37688..37915)
                     /locus_tag="KR432_000522"
     CDS             complement(37688..37915)
                     /locus_tag="KR432_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBH0639077.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            complement(37980..38252)
                     /gene="rpsP"
                     /locus_tag="KR432_000523"
     CDS             complement(37980..38252)
                     /gene="rpsP"
                     /locus_tag="KR432_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH0639078.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            complement(38290..39642)
                     /gene="ffh"
                     /locus_tag="KR432_000524"
     CDS             complement(38290..39642)
                     /gene="ffh"
                     /locus_tag="KR432_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH0639079.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            complement(39639..40007)
                     /locus_tag="KR432_000525"
     CDS             complement(39639..40007)
                     /locus_tag="KR432_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBH0639080.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            complement(40110..41390)
                     /gene="ftsY"
                     /locus_tag="KR432_000526"
     CDS             complement(40110..41390)
                     /gene="ftsY"
                     /locus_tag="KR432_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH0639081.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            complement(41417..>43740)
                     /gene="smc"
                     /locus_tag="KR432_000527"
     CDS             complement(41417..>43740)
                     /gene="smc"
                     /locus_tag="KR432_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH0639082.1"
                     /translation="MNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNENLKNVN
                     NRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNRGVKEV
                     LKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYLKQNNL
                     GRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTILINNI
                     DEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTEKISNI
                     KNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEIESLKG
                     SITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKRYNDLY
                     DKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEEHEIIK
                     LQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQHIELK
                     ESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESLKREIR
                     SLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFEIKFEE
                     ISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGGEKALT
                     AISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHRRGTME
                     AADYIYGVTMQEKAISKVISLKLKEAQEITDII"
BASE COUNT        13593 a         7835 c         4560 g        17752 t
ORIGIN      
        1 ttgtagatgg tttataataa aaaatggaag aatagcatag aatataaaag ttctatacta
       61 ttcttccatc gacattaagc aatagaagtt tattcttctg aatcgcttac tccattacac
      121 tcgtcacata atccataaaa tttaacatca tgatcaagta ttttaaattt gtacaatgat
      181 tgtatctctt tttctatatt gtctaaaagg tcttctttag cttccattat ctttccacaa
      241 tttttacaaa taagatgatg atgattatga cagtcttctt tatgtagact aatctcatat
      301 cttatacatc catcgtctaa gtttagtttt gatattaatc ctatttcatc caatagctgc
      361 attgttctat atacagtagc taagcctatt tcaggacagt caaccctaac taaatcatag
      421 atttcctcac tacttagatg tgaatggtca tgtttaagca atatttcaac tattgctctt
      481 ctttgtggcg taatcttaaa accagtttct tttaacttgt cttttaataa atccattgta
      541 tttgccataa catcaccctt actattttca attgataatc tttattaaat aatattgtaa
      601 ttgataattc attttttgtc aagacattta aataaattta atttattctc ataatataaa
      661 tatataccca taaaagatat attattcacc aaaattcata aattttttgg tatttttaat
      721 aaaaaacaaa aaaacagata tatactataa atgcttttac aattaataag gtttaattaa
      781 agcaaatata ataaatatct gatttttata tacaaactta attacttatc tagttaagtc
      841 catttgattc atcttcgtcg taatttaaac cttcttcttc cattatagca gtatacacag
      901 ctacaacagt ttcatactct tcatcacttt ccaaaggtat taatatttca ccttcttcat
      961 cctcatcaat tctaaagatt aaagcttctt cagcttcttc atcatctaaa ggcattaata
     1021 ttgcatactc ttttccttct gcttctaatg ttaatattat ttcaaattgg ctttcaacgc
     1081 cattttcgtc tattaaattt attatatttt cttccataat gtcattcacc tcaaatttat
     1141 ttttattttt attatttata ttatatatca taagtattca attttatagt tcttttatga
     1201 atttatttta aaatctaaat atccttgcaa aattattact gctgccaaca tatctataac
     1261 cttttttctt ttttgtctac taacatctgc ttctaataaa gacctctctg ccgctactgt
     1321 agaaagcctc tcatcccaaa actctacatc aatacctgtt tctgctttta ccaattcaca
     1381 aaacttgatt actttttctc cttgtggacc taaagttcca ttcatatttt ttggtaatcc
     1441 tgatacaatt ttatttactt gtttttcttt tattattgct tttaactctt caatatcttt
     1501 tttcttgcca actcttttta ttgtttttac accttgtgca gttaatccca tcaaatcact
     1561 cacagcaact ccaatagttt tatcaccaac atcaagaccc attattcttc catctaacat
     1621 agttcctcct ttatctattt atattctata gattaaatac atattctgaa cctctcataa
     1681 ctaaggtcac aggcttcctg cttcatagaa ttttgcataa tcaatcataa aaattgcaat
     1741 atgatttttt atgtaagcat ccagtttaca ttaataatca tattgcattt tttatttaaa
     1801 tcctatatga acagtaaaaa attttttagt tgtttttttc aaatattatc atatttatat
     1861 ttattattat tagtggtata agtatatata tagatgctgt agatgcagca actattccag
     1921 aatcattaac taaaagagta attatacaac caaccataga tgcaataaac cctttaaaca
     1981 agattggaaa attattcatt atctttctaa agtgtctaga tggtttaaat ataagtactg
     2041 caattactcc aattcctaca agtaaaatat ttacccatac acttgtctgt gctagttgca
     2101 tattcatttg aatttttctt ccaaatgttt gtaatatctc tcctggtcca tttaggaaaa
     2161 ttctctgtac aaacatacct aaatgagatt cactacctga aagtatatct aaagctgcaa
     2221 atgcaaaaac gacaactaca gctgaaacag ctaataatac agctttctta aaatctattt
     2281 taatgtcata tataagcatt ataaacaaca aatatgctac acattctgat atagctccac
     2341 caacatttgc acccattgaa ggatatgcac tagttataag tattattaac gaaaaaatta
     2401 caacaagtaa tttagatacc ttcttataat gtaataatac tgctaagcca aatactgcac
     2461 ttgctatagt tacaccttca tattcatttc ctacaccata gtacctagct cctatgacag
     2521 catcataact cattatgtta ttttgcatta agtatgttcc aaatacagaa tctataacta
     2581 tgactaaaat agtaagtgtt gcaaaaaatc ccatgtgctt tatatcattg tccttaaata
     2641 attttctacc taacaagtat aatattagcg tagtcgcaaa tattcccaaa gatattgaaa
     2701 ttggagtttt aaaattcatc aaaggagcta acataaatga tagtggcatt attatgccta
     2761 attttataaa ctccttaagt actttgaata cacgctcttt atgtggcata taatttctaa
     2821 agaaaatcgc aaacatggca attacccaag aagcagatat tacgcctaca aagccattta
     2881 taatagttga ccttattgaa gatatagaca ctatcctttc ataaagtgat tttagatagc
     2941 caacattgtc ctctttattt actaaagtat aacttctacc taccatctct ttattttcaa
     3001 gaccaaatgt acctagtata tctactccaa catctaaatt ggcaacaatt ccagcctttc
     3061 ttgttgtggc agattccagt agacctttcc cttctccact aaattttatt attggagaaa
     3121 gtcttctctt attcttataa tctaggtcac ttggaaatgc acttgctata taaacagtgt
     3181 cattttctcc aaccatagaa aatacttgtt caagatattt atttatattt tttacaatat
     3241 ttttcttcat tgtaccataa gtattatcat ttagatttaa tctatacata tctaatctat
     3301 atgtatctcc aagttctaca aatatagcat cactattttt ataggatttt ttagtttcat
     3361 ctagtaattt atcgtagtct gtagatattc caaatggcat tttagaatct ttcttattaa
     3421 tattatcaat attacctgaa tcaatccttc catattggtc catagctact aaacataagt
     3481 ttctattttt aactaattga ttattttcaa ttatatcagc atttccaatt acagatgtct
     3541 ttaaaccatt ttctgataaa gtttttccta atgaccctaa atttgctcca tattctccat
     3601 actcaagatt ttcatttata gatctattaa tgcctaaatc atttatttct ttagcttttt
     3661 gacctgttgc gacttcaaaa gctttagcag tatcttggtc tactttttga aaattgatat
     3721 agtcttcagt agttacacta gctcttgttc ctgcacccat acttgcataa gaccttctat
     3781 catcagtacc ttgatcccct cttatattca tgagagctac atagcctctc ttttctatct
     3841 catttttcaa gattggaata ttcatcatac ttccaagatt ggtcctattc atatcaataa
     3901 atatgacctt gcctttttct tcagcaaaac catagctagc agttgataca aaaatcataa
     3961 tgactattgc aaacaacgac ataatctttt ttctcattta tttgacctac tttctattca
     4021 gatagtgcgt gattacttct tcaagtatct catctctttc aaatttttta attatagctc
     4081 ttgcattttt atgactagtt atatagctag aatcaccaga aaggagatac cctataattt
     4141 gatttatagg gttatatcct ttttcaacta atgctttata tacaaaatca atagtatctc
     4201 ctacactcat cctatcttct ggtattcctt caaatttcat agtgtagtct aaatcttttt
     4261 ccaaactaaa aacctccttg agtgaatgtt atttaacttg tgtcttaata acctcacttg
     4321 catagcttaa agcttggtca accttagata catcactagc tcctgcttgt gccatattag
     4381 gtcttcctcc accttttcct ccagctattt ttgcaacttc tctaacaata ttacctgaat
     4441 gtattccctt atctaataca tctttagtgg cagtaactac aaagttaact ttatcatcta
     4501 ctacatttgc aagtactact acaccagaac caagcttatc tcttagatta tctgctgttt
     4561 ctcttagtgt atccatatcc ataccttcaa atttattagt tatcaaatta actccattta
     4621 cttcaacttt agaatcaaaa atagaatcaa ctgattgtaa actcatctta gctttcatat
     4681 catgcaattc tttagataag tttttatttt cttctaataa agaacttatt ctttgaatta
     4741 aattatcttc tttagatttt aagtttacac aaacttcaga gataattcca tctctttctt
     4801 ttaggtattc ataaacagct tttcctgtta ttgcttctat tcttcttaca cctgctgcta
     4861 ctccaccttc agataatatt ttgaacattc ctacttgaga agtatttgtc aagtgagttc
     4921 ctccacaaag ttctgtagaa taatctccca tagaaactac tcttacctca tcaccatatt
     4981 tttcaccaaa tagtgctgta gctccttttt ctttagcttc ctttatattc ataatatcac
     5041 attttatgtc taaagatgat aaaataacat tatttacttt ttcttctatt acttttaatt
     5101 cttcatttga tatcgcttca aagtgagtaa tatcaaatct aagtctttct ggagttacta
     5161 aagaaccagc ttgattaaca tgttctccta atacttctct taatgcctta tgaagtaaat
     5221 gtgtagcact gtgatttctt gcagctgaca ttctagtttc tctatctacg agtgtcttta
     5281 ctttgtcccc attacttatt cttcctgatt ttattatacc tatatgtttt atactattat
     5341 tagcaccttt tttagtattt aatacttcta aaactaagtt ttcattagtt atcaatccac
     5401 aatctccaac ttgtcctcca ccttcaggat agaaagtagt gttatctagt actatagata
     5461 ccttatcgcc ttcttctgca ctttgaacta gctcatcatc tttaactata gcttcaatag
     5521 ttccttcacc ataaatttca ctatacccat taaatgtact gtcaactgta gattctaatt
     5581 ttgatagagg gtcttccttc caactttctc catccatatt tcctctagca tttctagctc
     5641 tttctttttg cttttccatt tcttcattaa aagcttcttc atctacagat agatgttctt
     5701 cttctaatat ttctttagtt aagtctatag ggaagccata agtatcatat agtttaaaag
     5761 cttcttgacc acttaggaca gtctttcctt cattttttaa tacttctata taagagttca
     5821 atatttctgt tccttggtct atagtttcat tgaatttttc ttcttctatt ccaactactt
     5881 ttttaatata actttctttt tcaactaaaa ctggatatgc tttatcattt acctttataa
     5941 cttcatctat tagtttctgt aaaaataatt cttttactcc taataactta ccatgacgag
     6001 cagcacgtct aaggagtctt ctaagaacat atcctcttcc ttcatttgat ggaagcactc
     6061 catcactaac taagaatgtt acagctctta tgtggtcagt tattattctt attgatatat
     6121 cattttttgg attctctcca tattttactc ctgttaactt ctcaactgct tctagtatag
     6181 atttaattgt atcaacttca aagatagtgt ctacgccttg cataatacat cccattcttt
     6241 caagacccat acctgtatct atattcttgt tttctaatag actataattt ccttcttcat
     6301 ctctatcaaa ttgagtaaat acatggttcc aaaattctaa atacctatca caatcacagc
     6361 ctggtttaca gtctggattg tcacatccat attcttctcc tctatcaaaa tatatttctg
     6421 aacaaggacc acaaggacct gtacctattt cccagaaatt atcatctttt ccaagtctaa
     6481 ccattctttc ttctgggaaa ttcatttctt ttgcccatat atcataagaa tcatcatctt
     6541 cttcatatac agtcacccat atcttatctt ctggtatatt tagccattct gtaacaaatt
     6601 cccatcccca tttgattgac tctcttttaa agtaatctcc aaatgagaag ttacccatca
     6661 tttcaaagaa tgtagcatgt cttgcagtta tacctacatt ttctatatct cctgttctta
     6721 tacatttttg agatgtacac attctcacac ttggaggtac ttctacacct gaaaagtagt
     6781 ttttcaaagg tgccatacct gagtttataa gtaacaaact cttatcattg tttggtacta
     6841 aagaataact atttgctaca taatgacctt ttgactcaaa aaattctaaa aacttacttc
     6901 ttatttcatt taatcccatc ttttccatat catgttcctc ctaataaatt ttattttttc
     6961 gtttaaataa aaaacctcgt ccctataaat aaatttatag gggcgaggac aatcgcggta
     7021 ccaccctaat tatcatacaa agtatgatat cttcggaaaa ccttagctat aacgtaacta
     7081 ccgtcaatct ctacttaaat cttctttata aaagataaat tgttcaagat tgctactaaa
     7141 aggctgcttc aaatatcata ttctaagggc ttccagcatt tccctctctc tgtaagacat
     7201 caaatatttt actattcctt atcactgtat ttgtttttta attaatatac taattatatt
     7261 actaaatttt acaaaattaa tcaatactat attttaatat acaataaatt tattttataa
     7321 actttattta ctcttaatat actttattgc ttattttttt aattttttag atgtttttat
     7381 aaacttttct cgattatacc tccacctact acaatatctc catcatacat tactacagat
     7441 tgacctttag taatagctct ttgagcttca tcaaaaacta ttttaatagt atcttctcct
     7501 actctatgaa tagtagctgg tgatggtttt gctgaatatc ttattttggc ttgaactctt
     7561 aagggttctt ctaacgtatc tattgatata aagttaacat ctttagcaat aagttccttt
     7621 ttaaataaat cttcattgtc tccaagaact atggtattat ttattggatt tatgtctatt
     7681 acaaacattg gttttccaaa agtaattcca agcccttttc tttggcctat agtataatat
     7741 aatataccct tatgttttcc aagtatattg cctttagtat ctacaaaaaa accttcttca
     7801 atacgctttt tagaatgttt ttttacataa tttgcatagt cattatcttt aacaaaacaa
     7861 atttcttgac tatcaggctt attgtgtact gccatcccca tttccttagc aatttctctt
     7921 actctatctt tttcatagtc acctattggc aataaagtat gctctagttg ctcttgtgta
     7981 agattataaa gtgcataagt ttggtctttt ttatctgtaa cagatttttt tagcaaatat
     8041 cttcctgtac tttcatcctt ttctatcttt gcatagtgac ctgtagctac ataatcacaa
     8101 cctatttgtc tagccttctt ataaaaatca tcaaatttta tatgtttatt acaagctata
     8161 cagggattag gagtttttcc ttccaaatat tcatctataa aataatctat tactttttct
     8221 ttaaacactt ctctaaaatt taaaacataa aatggtatac ctattttatt tgctaccctt
     8281 ctagcatctt caacagcaga taaagagcaa caaccacctt caatttcaac aacatcatca
     8341 tcttgccaca acttcatagt aacaccaatg acatcatatc cttgttgttt taaaagatat
     8401 gcagctacag agctatcaac tcctccactc atacctatca ttactttttt attcataaac
     8461 aaatttcacc tcatcttttg cattacttat tattatacca tatagagcaa gtattgtact
     8521 tatttaaaat atagcttgtt ctattttcta aaaaaataat catctttatt atttgaatct
     8581 tatggattat tttgttaact aattaacctc taaatcaagt taaaatactt aatttaggca
     8641 ataaaaaata gccattttaa aaacagctat tttttatgtc ctaattatat cattagtact
     8701 tctttttaat cattgtaaac ttctcataac acaatcactt atttttattc ttcttcttca
     8761 acatcatggt catgagcatc atctataaca tatccatcca attctggtat atggatattg
     8821 ttcttttgag cataatctat aagagctgct tttacagctt gttcagctaa aactgaacaa
     8881 tgcatcttta ctggtggtaa accatccaaa gcttctgcaa cagctttatt tgtaagttct
     8941 aatgcatctt ttatagtttt tccttttatc atttcagttg ccatagaaga acttgctata
     9001 gctgaaccac agccaaatgt tttaaactta acatccttta ttacatctcc atctatgtct
     9061 aaaaatatct tcattatatc tccacatgta gggtttccaa cttcacctac accacttgca
     9121 ttatcaattt ctcccatatt tcttggattc ataaaatgtt ccataacttt atcactatat
     9181 tgcattatct tttcctccta ctcaatttat gtctattttt tatgctttat ctttctgtct
     9241 attttactaa accttgtttc ttcgcatcat cataaagtgg tgacatgctt ctaagtcttt
     9301 ctattacttt tggtaagttc tctagtatat aatctatatc atcatctgtt gtaaaatctc
     9361 caactgtaag tcttaatgaa ccatgtgcta tttcatgtgg taaacctatt gcaagaagta
     9421 catgtgaagg gtctaatgaa ccagaagtac atgcagaacc acttgaacca gcaattccta
     9481 acatatctaa taataaaagt attccttcac cttctataaa ttggaatgca aagtttgcat
     9541 ttcctggcaa tctattttct aagctaccat ttactcttgt gtaaggtatt ctttctaaca
     9601 caccatctat taacttattt ctaagacgag ttaaagtttc tacatgattt tgcatatttt
     9661 ccttagctaa ttctgctgct tttccatatc caactattgc aggtatattt tctgtaccag
     9721 ctcttttgct tttttcttgt gctccaccat ggacaaagtt atgaagtctt acaccttttc
     9781 ttatgtataa agctcctaca ccctttggtc catatatttt atgtgaagac atactcatca
     9841 aatctattcc taattcttta acatctactg gaatattacc tgtagcttgt actgcatcag
     9901 tgtggaataa tatcttatgt tttttagcta tttcggatat ttctttaata ggctgtatag
     9961 ttcctatttc attatttgca aacattatgc ttataagtat agtagtatct tttattgaat
    10021 cttctagttg tcttaagtct actcttcctt cgctatcaac atctaaataa gttatttcaa
    10081 atccatgatg ttttgctaag tattcacatg tatgaagtat tgcatggtgc tctatttttg
    10141 aagttattat atgattacct ttgtttttat tagcataagc tacaccctct agtgtccagt
    10201 tatcactttc tgaaccacct gctgtaaaat atatttcact aggttcagca tttataaggg
    10261 ctgctacttg ttctcttgct ttatccaaag catcttttgc ttctcttccg aatgtgtgaa
    10321 aactagatgc attaccaaaa taatctgtta ggtatggcat cattgcatcc aatacttctt
    10381 ttttaatagg tgttgttgca gaataatcca tatacaatct tcttttttcc attacattca
    10441 tctccaaatc tattttttaa tatttgttat atcattttct aatttatttt tgttatagtc
    10501 atttatcata tctttaagag taattgaatc tataacatct tctataccct ttttcatctt
    10561 ttcccaaact atttttgtta cacacatgtt tgagttttca catacatctt catctagtac
    10621 acagtcagat aatgcaactg gtccttctaa tactacaagt atatctccta ctgttatgtc
    10681 ttcagcattc tttgaaagca aataacctcc ttgagcacct ctcacacttt ttactagacc
    10741 cgattttttt aaagatgaaa atatttgttc taagtactga tctgatattt tttgcttctt
    10801 agcaataaat tttaatgata ctggtccatt gtcttgattt aatgctaatt caaacattgc
    10861 ttttagtcca tatctacctt tagtagacaa cttcatatat tatcacctct atatagaaat
    10921 ctaattcata ctgttttagt aggaattact ttcaagataa ttttattata ccctactaat
    10981 tttgtcaaga ttaattttaa ttttttattt tgaataaatt ccccattttc caatgttttc
    11041 aaatcctagt tttttatata tctttccagc ctcttcatta tcataaaaca aacaaggaat
    11101 tttgttttct ctcagtaact ctgaacatag tttaattagg cattttgtag caagtccttt
    11161 agctctatat ttaggatgtg ttcctactcc aattatcatt gcatgagtcc tattttctga
    11221 agtactcttt gccattgaca ccacctgttt gttaatctct atacaatatc ctctacctgt
    11281 ccttagtcca ttttttaaat tctctaaagt tgtactgtga aattcatcta ttaagtcata
    11341 aagtttcact gtctttttta gatttccaag cctaattctc ttaacctttg cattacaatt
    11401 attatctatc aaaaactttt tagtttgaag tttgcaaaaa tcaactacct ttaaattatt
    11461 aagtcctaat ctctttgcta aagtatctac agcacatatt ttcccagata tttcttcgta
    11521 attagtatta cgaagtagat tataaaaacc ttcaacatca tactctccat cagaataaaa
    11581 catcataaat tcaaaatatt taagtaatat tgcctttatt tcaccatgtt cacccacgtc
    11641 tgcccatata tttaaaaaat tatttccata tccatatctt tcaatatccc ctattataaa
    11701 caaattgtac tcaggttctt tttttaagta tttcataact ctactgtgat aagttgagtt
    11761 aagttttata atcataatat ctacccccga tacataaaat tcagtctaaa aatcactatt
    11821 tagatattct aaatattttt ttatattttt ctcagtttta ttttcttgtg gtatgtaata
    11881 aatactatct tttatgctat ctggtaaata ctgttgcttt acataatgat ttttaaaatt
    11941 atgaggatat ttgtatccac aacccctacc tatttcttta gaaccagaat agtgagcatc
    12001 ctttatacta tttggtatat ccttaacaaa ctctttatcc agttcatcca tagctttcat
    12061 tattgctata catgcagaat tagactttgg agatgtagcc aaaagaatag tagcttctgc
    12121 taatggtatt ttcgcttctg gaaatccaag ttgcatagct gagtctacac atgcctttac
    12181 aattacaata gcatttggat aagctaaacc tatatcttca ctagctatta ctaaaagtct
    12241 cctacatata cttatcaaat ccccaccttt tataagtctt gctagatagt gtatagatgc
    12301 ctgtgggtca cttcctctta tagatttttg aaatgcactt aaaatatcat aatgactatc
    12361 accatttttg tcatagttta taattttatt aaatgtagat gcccttatta catctgaatc
    12421 tatataagtt atattatcct tatttggttt agttgaatag actaccactt ccaaaatatt
    12481 taaggctctt ctcatatctc catcagaaag aattgctata tcttcaatag cattattatt
    12541 gcactcaata tccatatagc tatcttcatt taatacttca acagctcttt tcagtccttt
    12601 ttctatatca agtttttcta aaggtttaaa ttcaaataca gtagacctac ttaaaagagc
    12661 tttatataca taatgatatg gattttctgt agtgctagca atcaaagtta tactcccatt
    12721 ttccataaat tctaatattg attgttgctg ttttttatta aaactttgta tttcatctat
    12781 atataatagg actccattta cattatttat acttccaagc tctgatatca ccttttttat
    12841 atcttctaaa gatgaattag tagcatttat tttatagaag cttttattgc ttctttctgc
    12901 tattatttcc gctactgtag ttttaccaac accaggagga ccaaaaaata tcatatttgg
    12961 taaaaaatta gattgtaata tcttagacaa tatttttcca ttacctatta tatgactttg
    13021 acctattaca tcattcattg tttttggtct tattttatct gctaaaggca ttttctcact
    13081 cctttttaat atacttatca ttaccttaat aattcgtcta aaaaaggtat ctatttgaaa
    13141 tagatacctt ttctaatcta aacctataaa atatagatta ttgattgttt tttaattttt
    13201 ttaattcatc tgtaagtttg tcattaagta ttcttatatg agtacctttc atacctaatg
    13261 atcttgattc tatcacacct gcactctcaa attttctaag tgcatttact attacagacc
    13321 tagttatacc aactctatca gctatcttac ttgctacaag tagaccttct tttccatcca
    13381 attcagcaaa aatatgttca actgcttcaa gctcggagta ggacagagtg cctattgcca
    13441 tttgaactac agctttcttt ctcatttctt cttctaattc ttcacctatt gctcttaata
    13501 tttcaagacc aacaacagtt gcactgtatt cagctattac taaatcatca tcattgaatg
    13561 aatttgaata tcttgaaagt accaatgttc ctaatctttg accgcttcct aatattggaa
    13621 ctactgtagt atatttttgt aatcttccat gttcttcagg aaagatttct aatatcttct
    13681 caccatttaa attttctaat gtttcatcaa tttttaacac attttgagta tattcatctg
    13741 aaaatttctt ttgcttagta tactcatctt ctataactga actgtcttga acatcattta
    13801 aatgaagacc tagtacttta ccttttgcac ttactacata aacattagaa cttaaaacgt
    13861 cgcccaatgc tccggccagt aaatcaaaag agacactgct tccaccactt gtttgtaatg
    13921 ttttatttat tttccttgtt ttttgtaaca cttcacttgc catttaatca tctcctcatg
    13981 aaatttaggc cttttaataa ccttttatat tttctaatat tattttcaca tttaatgaaa
    14041 atttattttt ctattagttg tttacacttg aaagattatc atagattgga acctttttca
    14101 agagaattat gtgatttttt tcaattttca ctcttttccg tcattatgct cttttgtctc
    14161 tttattacat tctttattag aacacactgt ttttgtttca gatttagtca ctttttcaac
    14221 catatatgaa ccacacttct cacaaaactc tcctgttggc ttattccaag ctacaaagtc
    14281 acagtttgga taattagaac acccataaaa agctttacct cttttagatt ttctaagtat
    14341 aatatctcct tcttcacatt ttggacattt aacaccaatt ttattcataa ttggtctagt
    14401 tgttttacaa tcgggatagt ttttacaagc cataaacttt ccaaatctac catatttgat
    14461 aaccatatta gacccacagt tctcgcaaat ctcatcagtt tcttcatcca tatttacttt
    14521 ttctatattt tcaagagctt cttcaatagc ctctttaagt ggtgcatatg attctgatac
    14581 aatctttttc caatctgtat tgccttcttc tataacatca agtttacttt ccatatcagc
    14641 agtaaaatct acatctataa atttatggaa attattttcc aaaatatcag taactattat
    14701 tccaagttct gttggacata gacttgttcc ttgcttttca acatactctc tattcagtat
    14761 agtagctatt gttggagcat acgtacttgg tctacctata cctagttctt ctagtgtttt
    14821 tactaaacta gcttctgtat atcttgctgg tggttgtgta aaatgttggt ctggcaatat
    14881 attttctata tttaatatat ctcctttttc tatacttggt aaaattttgt cttctctttc
    14941 tgaaaaatta tataccttgg tataaccatc aaatttcatt tttgaaccag ttgccttaaa
    15001 tacaatatcg cctattttac attctacatt tagtatatca aacactgaat cttccatttg
    15061 actagctaca aatcttctcc aaattaaatt gtataacttg tactggtctt tgctcaatga
    15121 ggcttttata ctctctggag ttctatcaac tgaggttggt cttattgcct catgggcatc
    15181 ttgaaccttt ttagtatcct ttttttcttt tttatcacca gtgtttttat agtagttttc
    15241 tcctaaattg tttacgatat aacccttagc tttttctttt gcttcatcag atattctttt
    15301 agagtcagtt cttatatatg atataagacc aacagtacct tcaccttcta tgtcgatacc
    15361 ttcatataat tcttgtgcta tcatcattgt ttttttagta gtaaaaaata acttatttgc
    15421 tccttcttgt tgaagcatac ttgttgtaaa tggttttgga gctgatttac gtcttgattt
    15481 agattctatc ttctcaacct ttaaatcttt accttccatt tcttttagta tttcatttac
    15541 agcttcttca ttttctaatt caatcttttc atcattttta ccataaaatt tcaaagttat
    15601 atctttgtct tttactgtct tagcctcaac atctattgtc cagtattctt ttggaataaa
    15661 tgcctttatt tctttttctc tatcacaaat taactttgtt gtaacagatt gcactctacc
    15721 tgcactaaga ccttttctaa ccttttgcca taatatagga cttatttgat aacctaaaag
    15781 tctgtctagc actcttcttg cctgttgtgc atcaaccaaa tttatatcaa tatttctagg
    15841 atgtttaatt gcttttttaa ttgcatcctt agttatctca ttaaactcta ttctacaatc
    15901 ctcagattca tctaaattta gtatatgagc taaatgccat gatatagctt caccttctct
    15961 atcagggtca gttgctagat atacttgttt agatttttta gcttcttttt ttaattcttt
    16021 tattacatcg ccttttcctc ttatatttat atattgaggt tcaaaattat tttctatatc
    16081 tacacctaat ttacttttag gtaaatctct gacatgacca actgatgctt taactgtgta
    16141 atgacttttc cctaaaaatt tttctatagt tttagctttt gcaggcgatt ctacgatgac
    16201 taatgttttg gccataaaac catacacccc cactacctaa tcttaattta tacatttaaa
    16261 ctgtacgttt tattattcat ttctactact aactcattta gtactaattc attgattatt
    16321 gaatttacat actttatttc tataccagta taatcacaaa tactatctat atgcaagttt
    16381 ccttctcttt ttatagcttc aattatttgc ttagacttgg catttagact tatattatca
    16441 tatttttgat ttattttttt atcaataata ttaaatatgt tatattcatt taaaatatca
    16501 tctatatttt caattaattt tgcaccttct tttataattt tatgacaacc tctactcatt
    16561 tgagaattta tattaccagg tattgcaaaa acattttttc catgttcaag agcaaaatct
    16621 actgtgatta gtgcaccact ttttattgct gcttctacaa caactactcc atcacttaaa
    16681 ccactgataa ttctatttct attagaaaaa tggtacgggg ctactgtcga gttaatatta
    16741 tactctgata ataataatcc cccattttct aatattttat ttgataaatg taaattttgt
    16801 tttggaagag gattgtctac tcctgaacca agtactgcta tagttttaga ttttccactc
    16861 atacaaccta tgtgcgagta tgcatctatt cctatagcta atccacttac tatattaact
    16921 ccatattggg atagttgaca acttattgtt tttgcacaat taattccata ggctgtaggt
    16981 tttcttgaac ctactatagc tatattaaaa ttattattaa ctaaacctat gtcacctttg
    17041 tagaataata actttggagc gttgtatata ttctttaaat tttcaggata ttctttatca
    17101 tatttacaaa tatatttaat cgcttttttg taaagtagtt cttttatatt ttctaaataa
    17161 gcgtaacctc tatattttac tatatttttt ctaatattca agcttatatt ttttaaatta
    17221 tatatttctt tttcagaaaa atcaaatata tcttcaatat ttactatttc attttctatt
    17281 atttcaattg tttttgttgt tattccacca attgatttta accataaata agtatctctt
    17341 ttttccataa aatccacctc aaaaaactat ttaaaataac tataataagc ctttctaaat
    17401 gaaaaagctt ctataataca ctgtgaattt atcaagtctc tttcttctaa atctgctatt
    17461 gttcttgcca tttttaaaag ttttgtataa cttctactgc ttaggttgta tttattaaat
    17521 atcatctcag ccgtttttga agcttctttt tccagtttac aatatttaaa taaattgtaa
    17581 gctttaattt cactattatt ttttatatta tctttcttaa atctatttat ctgtattttt
    17641 ctagcatttt ctactcttct ctttattttt tgagagcttt cttcttgttt taaagaatta
    17701 aaatctgaat attttataga attaacttct acaaaaatat caaacctatc taacaatgga
    17761 cctgatattt tatttatgta tctatcaatt tcgttacttc tacatctaca ctctgtctct
    17821 gacatataat aaccacatgg acatggattc atagctgcaa ctaacagtac tctacaagga
    17881 tattttgcac tgtactttac tctggatata tttacatatc catcttctat aggttgtctt
    17941 aaagtctcaa gtatttttct atcaaattcg gctatctcat ctaaaaataa gatacctcta
    18001 tgtgctaaag caatttctcc aggtcttgca tccattcctc ctccaattaa agattgcttt
    18061 gtagttgtat gatgaggtgc tctaaaaggt cttttatcaa ttattccttt actttcattt
    18121 atcataccca atatactata aaccttactt acttctatca tttcttctat acttatatct
    18181 ggtaaaattg tccttactct ttttgcaatc atagtttttc ctgagcctgg aggtccaatc
    18241 ataaacatat tatgatttcc agctgctgca atttctgcac tcctctttac aaaataattt
    18301 ccttttacat ctttaaagtc ttcatcatat ttactttctt cattattaat cttagtatat
    18361 ttgcaatcta tataactatt atctttttct aattctccgt tctcttttct tgacttatca
    18421 tccaaaaaag acattatact tactttatcg acttttatct cttcgtttaa aaaatcgaca
    18481 cattctttta aacttttaat aggaattata tcaatttcat ctactaatag actttctttt
    18541 atgttctcaa ttggtataaa aatacgtttt atgttctgag tttttgcacc cataattatg
    18601 ggcaatatcc ctcttacttt tcttattttt ccatctaatg ataattctcc tataaacata
    18661 ctttctctta tgtaattttc atcttttttt attaactctc ttaaaaggcc tatacttata
    18721 gataaatcta aaaaagctcc ttcttttttt atatctgctg gtgataaatt tacaactatt
    18781 ctcgtgcttg gaaatttata tccactatta agaattgctg actttactct ttctcttgct
    18841 tctttaattt ctttgcctgg aagaccaaca atattaaaac taggtattcc attgcttaca
    18901 tctacttcaa ccttaactaa aaagctgtcg attcctacta agttgcttga attaataata
    18961 cttaacatat ttaatccttt ccaaaaacat tatctttttt aaaaagcatt cattatgtga
    19021 tttattcttt tttctaacaa atagacctct atgatgtcaa atcttatggg agtattattt
    19081 aagttatttt ttataatata gtattttgcg acaccaatta tcttttttct ttttttaaaa
    19141 tctacagatt cacaagggta gccatacttt atgttagacc ttgatttcac ctccacaaat
    19201 acaacttcat ctcctatatt tgctattata tctacctccc ctatccttaa tctataattt
    19261 ttttctagta tctttgcacc ttttgatagc agataattaa ctgctatttc ttctccaaaa
    19321 tcaccttttt ctttattatt cattatctac tccaatatat taaattttta cttttaaatt
    19381 atcgtcagta tacaatttaa ctattcaatt ttttgaaatt gattttattt ccattgatat
    19441 taataaatac ttctattgac tttattcttt tatgatatta tgctagtttt tataatgaaa
    19501 gtttattttt atggaggaat gtctttaatg aacagaaaaa aaacatttat ttttatacga
    19561 tatttatctc tatttatatt acttataagt tatttaaacc atactgaaaa tacattgtat
    19621 ccagctataa caatgattat attatttcta ataataaata atcaagttag atttttttct
    19681 ttatcaagtg agaataaatt tatacttatt tcttattttt tagagcttat tctaattttt
    19741 atattttgta agtatataaa gacttttaat ccactttact atgtttcttt aattttggat
    19801 gcctcatttt taataaagga aaaatataaa tatatattat tctctttagt tataatattt
    19861 tcttttatta ttggtttaga taaaaattta tatttagcac tagaatcaag ttctacatta
    19921 ctaattataa ccctcttttc tgtgtatcta gaaaacgaga atttatctaa attatacaat
    19981 caaagtcttt atgacaaact cagggtttct aaagatgaat taaaaaaagc taatgccgat
    20041 ttagagattt atgcaaattc tatagaagag ttagcaattt tgaaagaaag aaaccgtata
    20101 tcgagggaaa tacacgatag tgtgggacat tctctttcta cgacgataat ccaattaaat
    20161 gctatagaaa aactcttaaa agacaaacct ctcatttctg atttagttca tgaacttaga
    20221 gagtttgtaa ctgaaagttt tcaagatgtg agaagagcta tatctgaact aaaacccatt
    20281 gaatatgaaa attaccaaaa tttacttaaa atcagagaac tggttaatag ctttattaaa
    20341 cttacaaata tcaacgttag attaactata tcaaaaaata cctggaacct atctacaaat
    20401 caatctatcg ctttatatag acttgttcag gaatctcttt ccaacgcatc aagacatgga
    20461 aaagcaactg aaatcagaat ttttataaca tttaatcctt caaatcttat aattactatc
    20521 agcgataatg gtatagggtg taacaatata aaaagaggaa atggacttag cagtattagc
    20581 gaaagaatat atgaattaaa tggtaaagtt gaattcgata attctaatac tggattcaca
    20641 attagagcgt cttttcctaa attttcagga ggtgattttt ttgaataaaa tcaaagttct
    20701 tatagtagat gatgaaaaat tgattagaaa aggtcttaac attattttat catcttatga
    20761 cgatttagaa attgtaggag atgcttccaa tggatatgaa gcattagaat tttgtaaaac
    20821 aaatgatgta gacatagttc ttatggatat tcggatgaaa gtttgtgatg gagttctagg
    20881 cacaagactt ataaaagaat atgatagctc tatagcatta ttaattttga caacctttaa
    20941 tgatgatgag tacatagaag atgctatgaa atttggggct tcaggatatc ttttaaagga
    21001 tagttcggat gaagtattac atgaagggat tcgttcctcg ttttttggaa atattgtact
    21061 tgataaaagt gtggctgaaa aaattatgta ccctcaaaaa actatagaac aaaaacatat
    21121 ttctgatatg tataatttaa cagaaaaaga aatatccata attagattaa tcgcaaatgg
    21181 acttaacaac aaagaaatca gccaagaatt attcttatct gaaggtacaa taaagaataa
    21241 tatcaccaat atcttaggga aactagagct tcgtgatagg acacaactag ttatatttgc
    21301 cttcaaaaat aaaattgtaa tataaatata taggtaagaa atctataccc tagaaatttc
    21361 ttactcacaa actcatttat aataaatgaa aatataaagt gacttaagtc acttttttta
    21421 gtgacttata gtaatagaat tatgattcat aattctttat tatagtatta tagattttta
    21481 acttatacaa aattattttg ggaggaatta ttatggatat agtaagagta aataatataa
    21541 caaaaagatt taatgataaa ttagcacttg ataatatcag cttttctgtt aaagaaggag
    21601 agatatttgg acttattgga ccgaatggtg ctggtaaatc tacacttata aatataatta
    21661 caaatcttat gttaccaaat agtggcgaca tccaaatcaa tggtttagat ttgtcaaaag
    21721 attgtataaa agcaaagtct ataataggat tagttccaca agaattagca cttatagaga
    21781 ctcttactcc ttttgataac ttagagtact ttggcactta ctatggacta aagggaaaat
    21841 tgcttcaaga gagaataact gaagccttag aagtcacagg actcactgat gttaaaaaga
    21901 aaaaaatcaa aaaattatct ggtggtatgc aaaggagatt gaacattgga atagcacttt
    21961 taaatcatcc taaaatatta atattggatg aaccaactgt aggtgttgac ccgcaatcaa
    22021 gaaaccatat tttcaatttt ataaaaaata tatctaaaaa gcaaaaaacc acagtaatat
    22081 atacttctca ttatatggaa gaagttgagc atctttgttc taagattttt attatggatg
    22141 aaggtaaaga gattgctttt ggagataatg attatttaaa atcattagta tctacaaata
    22201 caaaattaat tatggaaata aaaaatataa atgctcaact tatatttgat ttaaaaaata
    22261 ctaaaggtgt tgtatctatt ttagaaaata attcattatt agaattaggt gtagacaaaa
    22321 aattacaact tacagatgta ttatctgttg ttgacaaaaa taattcaaaa ataacacgaa
    22381 tatcttatga agaacctagt ttagaagacg tatttttaaa tctaacaggt aaaaatttaa
    22441 gggactaatg gaggtgattt catgaaattg tacattagtg taaaaactat gctaaaagtt
    22501 ttaaaaagct cctttatgct taatttaata tatttcttag ccttacctct agttttagct
    22561 ggatttcttg ggatggtgac agaaagtata tttcaaaatc ctataaaaac agatttgaca
    22621 tcaattgtta tatatgataa agataataca aagctatcaa gtaatttgac aggatatcta
    22681 aaaaacgatt tatcttctat attaacagtt caaaaagatg attctaaggc agacttaaaa
    22741 cttactatac ctaaaggata tgagaataat ttattaaatg aaaaatcaaa taatctaagt
    22801 atagaaaaac ttaaatctaa tgataatatt gcaatattac ttcaagatat attagatact
    22861 tatcatgaaa aattatatct gaataattct caaaaattaa gttcagaaga tttctcaaaa
    22921 ttatttaata agaattctat tgatacctct ataataaaaa ataatgttaa acaaaactct
    22981 tatgagtact ttgctttagt ttcccttgga ttcttcgcaa taatttttat aatgaatagc
    23041 attttatcaa attatgtaag tgaatcaaaa ggactttcaa aaagactcta ctctatacct
    23101 gtaactagag tacaattttt aatttatgat tttataggtt tctggattta ctcatttata
    23161 tttttatcat tatacgtttt attttttaga acactcggtg taacattcaa aggtaacttt
    23221 gttatattag ctttactttg tgctttagct tcttatttta tgacttcaat atctactttt
    23281 ataactacct tttttagcaa gaaatatggt acagttatag tttatgcatt actcttcttg
    23341 caaacagttt ttggaggtat tttcagcata gtaaatgatg tgtttactaa gtttacaagt
    23401 ttatctccta cttatttaat aggaacattg ttcagtaatt atgagacgtt taaaactata
    23461 gattcaatag aaagtttaat ttttacatgc ctaattgcat ctacagtatt gattgcactt
    23521 tctattgtaa aagaaaaata taaatggagg gaggtttaat aatgattagg ttgactattg
    23581 caaacataaa aagatatata aaaaatcatg ctcttttagt taatatggtt ttgtttcctg
    23641 ttatattaat gttttatctt aactttttta ctggcaactc agaaaatcaa tcaatgtact
    23701 tttctccagt agcgattgta tccaattctt ctggaaaata cgaacataag ttaatagata
    23761 gttctaaatt aaaagaaaac tcatttggat taaacgaaca agacaaggct atggatctat
    23821 taaaaaataa taaagtttct gctgtcttca ttttagataa aaacttttca agcaatatag
    23881 ataacttaaa acgacctgag gttaaatgtt ttaaaactga aaatggtgga ggtagtcttt
    23941 gggcagaatc acaaatagaa tcttttatta ctaattcatt aaaattaaaa atagatagaa
    24001 atatagatga taaattgacc aagacaaata taattgataa cagttcaggt aagaataaaa
    24061 attcattttt aatagttttc ttgatatgtt atttcatgta cattaatgca gcacatctag
    24121 cttctgattt attttcatta aagaaatcta ataccttaaa aagacttgtt tctacaaagc
    24181 ataatgatat aaaaataatt ttcagcatat ttttagggtt attttttact caagcaattg
    24241 tatacacctt aactttagta tgtttcacat ctataagtaa ttttaaattg actctaaata
    24301 cattgatgat tgtactagca aatagttttg ttgctactgg atttgtattt tggatagcta
    24361 gggtgtttaa aaacgaaggt tctataagtc ttatgtctac gttttattct ttaataggtt
    24421 tagctatatc catatctaat atgataccat cacttgataa attatccttt atggctaatc
    24481 tttcaaagct tacaccattt tattggacta tagatgctat taataataat ggtaatatat
    24541 taataaatat aattgtttta attcttattg gaatggtatt tgtaacagcc ggaagtctta
    24601 acttaagaga ttttgctaga aattaataat tattaaatct agttagtatg ttattttata
    24661 caatactaac tagatatttt aatatattaa atcctcttat cttatatttt tatagccaat
    24721 tatatatatc tattattcta aactttttat tatatattaa tctttttgac ttgtcaagca
    24781 tttcgtgata taatattaat tattagataa ttaaagcatt gtacccaaat tgcaatatct
    24841 aaaattcagt ttggaggtat aaccatatgt ataaaatcgg tgaaagtgta atgtatccaa
    24901 aagaaggagc ctgttatata agtggtcttg taacaaagga tataaatcat catatacaga
    24961 aatactatga attgactgtt atttacaata gtaatcttaa aatttcgatt ccagttttaa
    25021 atgctgataa gattggtgta cgtccaatta tggatgaaaa tgaagttgaa aactttatcc
    25081 aatcccttga taaggttgat tgtttatggg tttttgatag aaaaaaacga cttaaactat
    25141 accatgataa atttcattca ggagatgttt ttgagattgt aaaattaatt aagatgctta
    25201 tgatacaaga taattcaaaa caactatgta gcacagataa ggactttcta aataaggctc
    25261 aaaggtttgc tttgtcagaa ttggctgcag cacaatgcaa atcctacaca attgtattag
    25321 aagaaatgaa aaaccatata ctaaattcaa aaaataacaa ttaaattggt cttatagact
    25381 agtttaattg tttaactatt catacaatta ctctattttt cttcaaacct tttaatctaa
    25441 cattgataaa gatagattaa ttatatagac atctttattt aaataaaaaa atctaggggt
    25501 aaatcatatt cttgattttc cctagattta gtaataaaac ttactcttcc tcaagttctt
    25561 ttttctcttc cagtaaaaca cctaattcgt ttgcttctaa aacttgtgcc tctgcaatat
    25621 caagtaactc ttgttgccat tccacacatt tatctttgta atcaaaaggt agcgttctgt
    25681 caactatgta cttatcaata cagtcctccg cacttttaat tatttctttt ctttcatcaa
    25741 taaatttttc taattctact aaggcttctt cataagcttt tctactcata taaattcccc
    25801 ctttgtacaa ttattctaca ttaaaatgat ttttcctctt ttttgtataa aattttattt
    25861 gtatataact tttccatcaa tatcttttat agtttgacat ccagttaata tcatagaact
    25921 acttagctct gattgaagtt tatttacata agtttctaca ccatcagttg caccaccaaa
    25981 tgaagcagtc acaaaaggtc ttcctattaa tactgcatct gcacctaaac ctatcatttt
    26041 tagtacgtct aaaccagttc tcactccacc atctgcaagt attgtaactt tacctttaac
    26101 agcatcagca atttttggta aaacttcaca tgctcctggt gtacagtcaa gcactcttcc
    26161 tccatgattt gatacaacta ttgcatacac acctgcttct actgccatta aagcatcttc
    26221 aacagtcatt atacctttca atataaatgg tagtttagta gactttacta actcctttat
    26281 ttgtttaaca tttttaggaa gtactggttt tccatgtaat gataaagtta ctagaccaca
    26341 ggcatctata tcaactccaa ctgcaaatgc accagcttct tcagctaatc ttatcttttt
    26401 tataatattt tcattatccc aaggtttaat aaatacaatt ccagctccat tatacttttt
    26461 gactacatct aaattttcca ttaaaaaagc atctactgcc gtatcaccaa ccattgcata
    26521 aataccacta ttagcacatc ctgcaactac tggttctatg taatctcttt cattaatttt
    26581 tccacccata tttaaagtag ttcctgttac tggagctgca aatataggtg cactcatttt
    26641 ctttccaaac atctctatag atgtatcagg atttgataca tcatgtatta ctctcatatt
    26701 tacttttact ttttctaaac tcttgctatt ttctataaaa gaagaaccac ttcctttacc
    26761 tcccatgcca ggcacttcac ctgcacaagc aaccccatta caaaccttac aaactttaca
    26821 acttccattt aaattttctc tagcactttt taataattca ttatattcca tctttaatcc
    26881 cccattaact atagtaattt atattatata cattatatca tattatattt acagtatatt
    26941 tttcaaaaaa ctttttctgt ggataggagt tatgccatat ttttcaatag cttcatagtg
    27001 ttctttagtt ccatatcctt tgtgagattt aaacccatat tctgggtata ctctatcata
    27061 ttggtacatt atactatccc tagtaacttt agcaagtata cttgctgctg ctattgatat
    27121 agattttgaa tctcctttca caattggatt ttgagatatg tcaatacctg gtattgttgc
    27181 tgcatcaact aataaataat ccggtgcttt ttttaaacaa tttatagcct ttttcattgc
    27241 catatatgta gcatttaata tattaaattc atctatttct tcattattta ctatacctat
    27301 tccataatct agtgcttctt cttttattac ttcaaaaagt tcatctcttt ttgcctcact
    27361 cagtttctta gagtcattta caccttctat ttttgtatct tttttaaata ctacaacaga
    27421 agctacaaca ggtccagcaa gtggccctct tcccgcctca tcaattcctc caatatacaa
    27481 ataaccttta tcatagcctt cgttctcaaa tatatttata gtttccaatc tttcttcttc
    27541 ttttctaata ttatccagct tttttgctag ttttatagct agaccttgta cagattttct
    27601 ttcatcaact cttaacagtt ctatatattc catatatttt tctacttcta aagtctctat
    27661 tatttcttta atctccctca cacttttatc ctgcatacca ttccctcatc tttcgttttt
    27721 aaaaatttaa tttttaaata tattaaaatt ttttaattag agtttaattg catccactat
    27781 agccacattt taaacatata ctacatccac ctgtatgtgc aattccttca cttccacatt
    27841 cagggcacac aaatttttca tcttcttcaa aatttttatt tattatttct ttggcttttt
    27901 catctttatt atctttttct tttttattat ttttataata ttctttttta ctattttctt
    27961 ttttatcttc tttattattt tcttcctttt tttcatttat taaaattcca ctccttttac
    28021 atccatctct aaatattgtt actcctttaa gaccagcctc ccatgcctta atatatatat
    28081 tgaaaacatc ttcaactgta gtttcaaaag gtatatttat tgtagatgat atagaagcat
    28141 caatatactt ttgccatact ttttgcatgt ttattctttc ttcatatcct atattcatag
    28201 cagtaacaaa aaaatctggt aaacattctt catctgtaat acctttcttg tccatatact
    28261 ctttaacaat tggagtatat actttataat acacatcctc atcatgcaag ctttcagttt
    28321 ttcttatata tgaaagatta aatataggtt ctattccacc actgatacct atcattgtag
    28381 atatagaacc tgtaggaggt atggttaaaa gttgtgaatt tctaagccca tactttttaa
    28441 ccatttcttt agtgtcttca tttagatttt ctataaaaaa cttagattta gaaatatttt
    28501 catatttata ctcactaaat gttccatatt ctttagcaat taatgctgat tgcttcacag
    28561 cttcatttaa catagtctta ccaattaaat cacatagttc taaagattca tcagaaccat
    28621 atcttattcc catttttata agcatatcag ctatacccat tacacctaaa cctatctgtc
    28681 tatacttatg tacactatct ctttgttctt gtaatggatg caagttcaac ccctcatcta
    28741 agacatcatt taaagctact acagcttctc taacgcaaga ttttaattta ttaatatcaa
    28801 aagtagcatg ttcaccaaat ggttgtatta taaactctga caagtttatt gagccaagta
    28861 gacaacttcc tccacttgga agtggttctt ctgcacaagg atttactcct gaaaagcaaa
    28921 agttcttatc ttcactcata aaattatatg tttttatttt atcccaaaac aatatacctg
    28981 gttctgcata atcccagtta tttttcacaa atttattaaa taaatcataa gcatttacta
    29041 tcttaactat tttttctcca gtagtttcaa cttcaaaagt acaattatat aacttttttt
    29101 cccttacagc ttgcataaat tcatctgtta cccttatgga aatatttgct ttagtaacct
    29161 tctccaaatc tgtttttata tctataaact cttctatctc cggatgattt acatccatag
    29221 aaatcataag agctcctctt ctccctcttt gaccaattaa gccagttgtc atactgtaaa
    29281 gttccataaa agagactgct ccagtcgtat gttttgcagc attgttaact ttcgctcctt
    29341 ttggtcttag ctttgaaata tcaattccaa ctcctccacc ataactaaaa gttctagcaa
    29401 gtttttttgc agtatcaaat atatcttcta tgctatcttt tggtggttct attacataac
    29461 aattagaata tgttactttt tttccttctt tctgtaatcc tctatttgct aaaattcttc
    29521 cagcaaatag aaatcttttt tgtcttatta gttttgatat tcttgaacta ttatttgaaa
    29581 ttctctttat ccattcatca aaactctcac cattatgtct atacttgttc ttccatattg
    29641 atttttgtaa ttcatcaaac tcccaagctt tagtccttat ttcagagcgt ctttctctat
    29701 ataatatgta gtgttttgct atatcttttc tatttgattc cataagtagt acttcaacta
    29761 tatcttgtat ttcttctacg gataagcatt tttccataga attatagtca ttagctattt
    29821 ttcttgatat atcatatgcc aactctctat ctactccacc ttcactattt atactagctt
    29881 tatagacagc atcttcaatc ttacttttac taaaaagttc ttttgtacca tctcttttta
    29941 ttatgtattt catttttaaa cctccaataa attagtatta aattaataca cacattaact
    30001 ataactctaa acaaactttt ttatatattt gtatattttc atattaatta tctaacataa
    30061 tatatattat actattttat attcaatata acactcctta tagttttctt aacttgtaaa
    30121 atatcttcct aaaaaactca aaaataatat aaaaaagtat ataaaagtca aattatactc
    30181 ttatatactt tattttttta tatttttttc attttaactt tgattaatta ataagtattc
    30241 agaaaataaa ttctttattt tttctttaca aaaatatata ttgatgtttt tatattaata
    30301 ttaaacattc ataatatata atttaatttt atttataatt ataaataaat cttttttatt
    30361 ttattgtctt tataaatcat actcctccaa aaataaattt atcaaatgag attaataatg
    30421 ttcttttctc cacaataatt taataattct tttaaatata aaaaggaatt gccatataat
    30481 ttaaaggcaa ttccacaatt atcaattagt tttattatta tgcagtttta ctgcttttag
    30541 atggaataaa gtttcctatt attcctgcta ttacaactgg aactatccag ttaaatccta
    30601 atgaagcaaa tggtaagcta ttaactagag gaatttttac accaaaactt gttgcaactg
    30661 ttaatacact tactaataat gccatgtatg cagcacattt gaacacgttg tcatttttaa
    30721 ttttattact aaataatgct agtataacta gagttatagt tgctggatat accatactta
    30781 gtataggaga agagaatttt attattgtac ttactccaaa gtttgatatg attgcactaa
    30841 atacacaaac tactataact atatgctcat actttaattt tccatctgta agtgttgaga
    30901 aatattgacc agctgctgaa gaaagaccta tagctgttgt taaacatgct aatgcaacta
    30961 taatagccaa taaaatttta cctgtagttc caagtaaagc tgctgttatt gcaactatta
    31021 aagctgtttg ttgaacatct tgtccatatt gttgagacac agttgctcca agataacata
    31081 atccaccata aactaatgcc attcctatta ctgcaattac acctgcactt attgtggctt
    31141 tcatcttttc tttctcacct gtataacctt tagctattac tgacatgatt ataattgatg
    31201 caaatacact agctgccaat gcatccattg tttgataacc ttgagttata ccttctgcaa
    31261 atacattttg aattaaagaa ctgtctctta tttctcctaa tggagatacg atacctttaa
    31321 ctattaatac tgctaaagct ataagtaaag ctggagttaa tattgaccca actatatcta
    31381 caacttttga tggtcttatt gttaaaacca atactagagc aaagaatact atagagaata
    31441 ttactggact tattccagaa cctattgtag gcaatatacc catttcataa gttgttgctg
    31501 ctgttctagg tattgctaaa agtggcccta tacagattac cattgcacat cctaatgcaa
    31561 tacttaatcc tttgcctgct cttgagaaca tcttcgatgt gtctccattg aacttagttg
    31621 ctgctataac tgctaataat gccaaaccac catctgcaaa aaggaatcct atgaaagcta
    31681 ttagccaact acttccagat aaaactccta gatatggagg gaagattaag ttccctgcac
    31741 caaaaaacat tgcaaatagt gcaaatccaa ttacaattac atctttaaaa ttgatttgtt
    31801 tctcacccat ttaaagcacc cccatttgaa ttttactacc ttttactaaa aaataagtcc
    31861 aatctcttca ttaaatgaaa accttttgac gagattttat ttaattatac atttttgact
    31921 taaatttgtc aattatgttt cataatttat aaaatcttca caatatttaa tacaactttc
    31981 ttaattaaac aaaatcaacc tgtatacttt aaacatagtt ggttttttgt tttttctcta
    32041 attttccttg ataaaattaa ctattcagaa caattaaaat attaagactt tttagattat
    32101 tcgttttatt attaagttgt attgctttat atactgtata tatcactctc ctttttacat
    32161 tcatataatc tttttaattc aatttaaatt ttaaatattt tgtgtttatt atataatctg
    32221 aaaaaaaagc agtcaatagc atttttaatt tttttaattt tcaaaattat gattcttaaa
    32281 aaatcgtttt ccttttatca taacacctat taacgttaat tcttgtagta ttttgtattt
    32341 attttgctaa attgacagtt aactttattc aatttttatt ctactcacgt agatttacct
    32401 cttgtctttt agtcttaaaa ccattttttc tgaaaattcc ttattatgat ttatcaactt
    32461 atttttcttc atagcaatta ctttctaact tccaacttac ttaaactttg cagttgctag
    32521 tattcacgtt tacatactta tatgtcataa ttaactaaaa attcttaata tatctctcaa
    32581 tgaaaacaat ttactcattt atttaataca tttatataat catatgtctt aaattttgtt
    32641 ttattatttt attattgtca caattttatt tatattatat ttttgtaaaa atcatttaat
    32701 atgtatactt aaatttataa aattactcta aaattctttt ttaatctcta ttattgttaa
    32761 aaaataaact atatattttt ttgatatttt cttatacttt ttccatatta aatacaaata
    32821 attaattata gaatcccaaa ataaaaggag ttatttgtac aaataactcc ttttactttt
    32881 agttaactca tttatttaca attgattata ttttaattaa acaaaactta gtttgttcca
    32941 agtgtattag attgactctt tgatggtata aaattaccaa ttattccagc tattataact
    33001 ggtaccaccc agttaaatcc taatgaatga agaggtaaag attttactac tggaacatct
    33061 actccataac tagatataac tgttagtata cttacaagca atgacatata tactgctcct
    33121 ttgtacacat tatcattctt aattttttca ttgaagaaag ctaaaattat taaaacaatt
    33181 gatggtggat aaactatact tagtatagga gatgctatct ttattatagt tccaacacca
    33241 aaagatgcta ctactgcact gaatacagat acagctaata ctattttttc ataactcaat
    33301 ttaccttttg taagtctaga gaaatattga cctgtggcag atgtaagacc tatagatgta
    33361 gttaaacatg ctaatgatac tactactgct aaaatcgctt tcccaacatt tccaagtaat
    33421 ccttcagtta tatttactat tacttgtgat tgtactgcat ctgttccata cattgttgat
    33481 acagttgctc caaggaaaca taatcctcca taaactagag ctaatcctcc acaagcaaga
    33541 acccccgcct taccaatcat ttttaacttg tcactaatag tagaatatcc tttatcattt
    33601 aaagatacta ttaacactga tgcaagtgct attgctgcaa aagcatccat tgtctgatat
    33661 ccttgaccta taccttctgc aaatacatta ggtatcatag gtttatctac tatatctcca
    33721 agaggtgata taataccttt tataattata aatgctagag ctattaaaag agctggagtt
    33781 aaaaactgtc caactatatc tacgacctta gaaggtctta ttgttagtac atatgaaagc
    33841 gcaaaaaaaa gtatacagaa taataacact ggaattgaag ttccaaataa aggcattata
    33901 cccatttcat aagttgttgc acctgttctt ggtatagcta agaatggacc tatacataaa
    33961 atgtctgcac aacctataag aatccctaac ttcataccac atcttttaaa catttcaact
    34021 acgtttccat cgaatttagc tgtagccatt actgctagaa gtgccaatcc agcatcagca
    34081 aacgtaaatc ctaagaaggc aattaaccat tctggacctg taattattcc caagtaaggt
    34141 ggaaatatta agttacctgc tccaaagaac attgcaaata acgcaaatcc aaatactata
    34201 acatccttgg tcttacccat tataaaatcc ccctttttaa ccttattaaa ttaataaaat
    34261 gtctcgattg tttacgcttg cataaataaa ttaaattaaa caataagaac ttattattaa
    34321 attaataaaa ttaaataaat taaattttta atatattgta tttatttgtg cttattatat
    34381 attatacgaa acaagcaagt caatatgcta aaatttaaaa taatcacact cattttaatt
    34441 ttcaaaatat taggtgtatt tgtcttaaat taatattttt aaaggttttc taacctaatt
    34501 tatactttta tatggagata tttgtctata tttcaaaaaa ataaaaaagc ctttgctaaa
    34561 aggctttttt atttttttaa cattaaatct ttgattttgt tttaaaatct atcttttaac
    34621 gttttctgga acttccaatg ttatatttcc tatttttcca tctctaaatt catttaatac
    34681 agttgcggct atgcgtgtat aatctagttc ttttttacct gttataaacc ctcttttatg
    34741 gccaatcata tccatagttt caattggagt ttctcctaga caatctaact tatatctggc
    34801 tttcaacttc tcaggttcta ttttcataag tttttctata agtcttaaag ccaaagtctc
    34861 agtatctaaa atctcatcct ttatagctct actaaatgct aaatttaatg caacttcttg
    34921 gtcttcaaac ttaggccaaa gtattccagg tgtatctaaa agttcaagat ttccctttaa
    34981 tctaacccat tgtttccctt tcgttactcc tggcttatct ccagtttgag tgctttttct
    35041 acctgttaat ttgtttataa gagacgattt accaacgttt ggaactccaa ctatcattat
    35101 tcgtatagct ctttcttttc tccctttgtc ttttaaagaa ctcatttttt ctctagttac
    35161 atttttacat tcttcgatta tattatttat accaacacct tttattgtat ctactggtat
    35221 agctttgata ttaattttct tatagtattc aatccattga ttcaatttat ccctatctgc
    35281 catatcactc ttattaagta cgacaactct tggcttatct cctacaagcc tgtctatgtc
    35341 tggattttta ctactaaacg ggattctagc atccaaaagt tcaattacta catcaatcaa
    35401 ttttaagtta ttttttacta actccttagt ttttttcata tgacctggat accagtttat
    35461 atgtagattg tcatccataa gatactcttc gtattcattt gacacatgta tcactcctta
    35521 ttaatattat attattatat tctaattcca cttaaaaaat ctatcttcac aaactataat
    35581 attgtcatct ttttaacgtc atactttaca taatactgtt ctcttatttt taatatcgtt
    35641 acaaaatata atccaatatt taccaatttt atttttcatt ttaaaagcta taaattcata
    35701 gataaataat gtatattttc aacaaatatt tctatttatt tctagtataa ttatttgctg
    35761 taattttcat taaaatagaa agaggactga taaatcagcc ccccatttat aagtaaagtt
    35821 tttaattatt tatttctagc ttcttttatc ttagcagctt tacctactct acctcttaag
    35881 tagtttagtt tagctcttct tactttacct tttctagtaa cttctatttt ttctattttt
    35941 ggagagtgaa ctgggaaagt tctttctaca ccaacattga aagacatttt tctaactgtg
    36001 aaagtttctc ttgctcctcc accttgtctc tttaatacta caccttcgaa tacttgtatt
    36061 ctttctcttt ttccttctat aattctaacg tgtactttaa ctgtgtcccc aggtccaaaa
    36121 ttaggaactt cattttttaa ttgttcttgc tcaattgctc ttagcatttc attcatcttt
    36181 cttcctccta gatatcatag acgttcttaa ctacccgacc agtgtaaaca gaggaacgcc
    36241 cgtattttca acattttata ttatactata cttcaatagt tattacaagc acttttatgt
    36301 ttttaattcc atttataggt actttattgt atacatatat attaattttt cattatattt
    36361 ttatttttca ttagactttt tatataaatc aaatcgtctt tctttggtta tccttataga
    36421 ttcttctctt ctccactcat ctatcttttt atgattccct gataaaagca cttctggtac
    36481 ttttaatcct tcaaaatctc taggtctagt gtaatgagga tattccaata agttatcttt
    36541 aaatgattct tcttcaaatg attcttcttg gtttaataca cctggtatta atcttgatat
    36601 agaatctatc attataagtg ctgggagctc tcccccagtt aatacataat ctccaattga
    36661 aatttcatca gtaactatta aatcaatgat tctctcatct actccttcat aatggccaca
    36721 taatagtatt atatcttctt ttaaagacat ctgctttgct atagattgat tgtatacctt
    36781 accttttgga gtaagataaa ttacacttgg attacttata ttgtgagttg ttattatatg
    36841 cttatatgtg tcatatatag gttgaggagt cattaccatc cccactcctc ctccaaatgg
    36901 ataatcatct acttttttat gtttattatt agaaaaatcc cttatattat atatataaac
    36961 ctcaattata cctttttcaa ctgctctctt cattatactt tcatccatgt acgaattaaa
    37021 tatttctgga aaaagtgtca ttatatgaaa tctcatatta ccacctaatc tatcattccc
    37081 ttaatagggt ctataatcat ttttctttca tcaatatcta ttgtaggtac aaattttttt
    37141 atagctggta tcataaattc tttatcttct tcacctttta ctacataaac atcatttgct
    37201 gagtattgaa gaacatcttc tagtattcct acatattttc catctacagt taaaacttcc
    37261 attccaatca tatctgctat aaaaaattcg ccttcttcaa gctctctgct atcttctcta
    37321 gatacataca taaatttatt ttttagtttt tctgctttct ctatgctgtc tatgcctttt
    37381 atcttcatta taaccataga acctttgtat cttacttttt caacagccaa tttattattt
    37441 aaatcttttc ccagatagaa atcttttaat tcatcaaacc tctctatatc atcagtaagt
    37501 ggataaactc ttacttcacc ttttaaacct tgtgtattta ctatttgacc taccttaaaa
    37561 tgagttaatt tttctttcat gttatcacct tcttaaaaaa tttaatctta tgtggtcata
    37621 tcatcataaa atcttaaact acgcatttat ataaaaagga ctaggcatta tacctaatcc
    37681 tcctatttta tactatctca agagaaactt ttatattttc tctatttgca acagatctta
    37741 cgacagttct tatagcttta gctattcttc cctgcttccc aataacctta cccatatcct
    37801 cttgagcaac tgtaagcttt aagacgatac catcattatc ttcaaattcc tcaacaacaa
    37861 ctgagtcagg attatcaact agagccttag ctatatctac aactagctct ttcatatatt
    37921 tcatctccta aagtaacctt agcaatgagc ccttacttaa actaattgcc aagaaaaaat
    37981 tattttgctt gtttagaagc ttcgaatttt tctattactc cgttttttac aagtaatttt
    38041 ttaactactt ctgttggctg agcaccagta gctaaccatt ttatagcttt ttcatcattt
    38101 attcttactt gtttaggttc agaaattgga ttatagtatc ctatttcttc tataaatttt
    38161 ccatctcttg gagctcttga atcagctact actattctgt agaaaggttt tttgttagct
    38221 cccattcttt ttaatcttat tttaactgcc attacagtca cctccgaata atttttactt
    38281 ttttatattt tatttaaaaa aaggtaaacc gcctaagccg ccccttttct tcatactctt
    38341 ttgtgaacca gcaaacatct tcatcatttt tttcatttca ttaagttgtt ttataagtct
    38401 atttacatct tgtacactag ttccactgcc cttagctatt cttttctttc ttgaagcatt
    38461 taatactcct gggtttcttc tctcatatgt agtcattgac tgaattatgg cttttgttct
    38521 cttcatttct ttgccattca aatctacatc tccaagttgg tcttttacat ttcccatacc
    38581 aggtatcata ccaagtatct tgtcaagagg tcccatattt tgtatttgtt ccatttgttg
    38641 taagaaatct tcaaaatcaa gctcttgttt tttaattttt tgctctaatt ctttagcttt
    38701 ctctaagtct atattttctt gtgctttttc tattaaactt aagacatctc ccatacctaa
    38761 tattcttgat gccattctat ctgggtggaa aggttctata tcatctagct tttctcccat
    38821 ccccataaat tttataggct tttgtgtaac tgctcttatt gaaagtgcag caccacctct
    38881 agtatctcca tccaatttag ttaatactac tccatcaaca ccaagtgcct cattgaagct
    38941 ttctgcaaca tttacagcat cttgacctgt catagaatct acaaccaaaa gtatctcatg
    39001 tggcttaact tctgatttaa tagactttaa ctcagccata agtacttcat ctatatgaag
    39061 tctaccagct gtatctataa taactaaatc attagcattt ttaatcgcat ggcttaaacc
    39121 tgctttcgca atatttactg gattttcttt atctcccata ttgaatacag gtatatccaa
    39181 tttttctcca acaacttgaa gttgctttat agctgccggt ctataaatat cacaagctac
    39241 taatagaggt tttttacctt gctttttaaa atatcctcca agttttcctg ctgtagtagt
    39301 cttacctgca ccttgcaatc caaccatcat taaaattgta ggaggctttg aggaaatcat
    39361 tactttactt tgtacatctc ccattaaact tgtaagctct tcatttacaa ttttgattac
    39421 atgctgagct ggagttaagc tttgcataac ttcttgtcca acacatctct cttgaacttt
    39481 ttttatgaaa tctttaacta ctttaaagtt aacatctgcc tcaagaagag caagtttaac
    39541 ttctctcata gcatttttta catctacttc tgtaagtttt cctttagatt ttaacttact
    39601 aaatgcccct tgtagtttat cagacaatcc ttcaaatatc attttatcat ctccctacat
    39661 acatcttcta tattttctag cttagggatt aaattagcac aatctctatt gtgcaataaa
    39721 tcttctttaa tgtcaataat tttatctttt ataatcttaa tatttttttc ctgctcttga
    39781 atctttgata ctaagtgtaa cttttcttca taatctctta gtattttttc tgaacgcttt
    39841 aaggtatcat aaacaccttg tcttgataca ttcagatttt cactaatttc acctaaagaa
    39901 taatcttctt catagtatag agcaactatt tctttctgct tgtcagttaa taattcttta
    39961 tactgttcaa ataacaatcc aatttcgacc attttttcta tattcatatc ttacacttcc
    40021 tagcagtata ctgtaaaggt tatttacttt acactattgt attttatatc ataacaacac
    40081 tcttgtcaac cattattttt aaaagaacat tagttaccaa ataatgcatc tgaaaatgat
    40141 tttgcattga aatcttgtag gtcttctaca ctttctccaa ctccaataag ttttacaggt
    40201 acatctactt ctgattttac tgcaaggact actccacctt ttgcagttcc atcaagtttt
    40261 gtaagtacta tacctgttat atctgcaact tctttaaatg tctttgcttg aacaactgca
    40321 ttttgacctg ttgtagcatc caccacaaga agtacttcac gttttgcttc tggaaattct
    40381 ctatctacta ttttaaatac ttttccaagt tcattcataa gattagcctt attgtgtaat
    40441 cttcctgctg tatcacatat taatacatct gtttttctag cttttgcagc ttttatagca
    40501 tcaaatacta cagctccagg gtctgctccc tcttgatgtt ttattatgtc tacattggtt
    40561 ctatttgccc aaatttctaa ttgctctgtt gctgctgctc taaatgtatc tgctgctgct
    40621 aataaaactt ttttaccatc ttttttgtat ctatttgcta gtttacctat tgtagtagtc
    40681 ttaccgaccc catttacacc caccattaat attatacatg gtgctggttc tatatctagt
    40741 gtagagtttt catttgtaag aatatcttca actatagatt taagttcttc tcttaccttt
    40801 attggttcag taataccttt ttgctttatt ttatctctta gtctttcaat tatatccata
    40861 gttgtattta caccaacatc tgctgttatt aaaatttcct ctaaatcttc aagtaattct
    40921 tcatctattt ttgtatatga ttttaaaact tcatctattc tatcagttat tccttgcttt
    40981 gcttttgtca atccttgttt tagtctttca aacaagttaa ctttcttatc ttctttatct
    41041 tctatctctt tagactcaat ttcatcataa ttttctaatt cttcgattat ctcttcttga
    41101 gaatcattat ttgaaatatt ttctacttct tttgcattac tatccatctc aacttctatt
    41161 ttttcaaaat tttcgatttc ttcagtgcct tcattattga cactttcctc agttttttct
    41221 tcaatatctt ccactttatc tatcacttct tcttcaagtc ctgaaaaaat ggtttcttcc
    41281 aagttttcta aactatcaac actgtcttca acttctattt cttcttcagc atcttttttt
    41341 tctatttctt tttccttgtc ttttccaaaa ccaaataatt tttttaacac tgttattcct
    41401 cctatgtcaa tctaagttat ataatatctg ttatttcctg agcttctttt aattttaagc
    41461 taataacttt tgaaatggct ttttcttgca ttgtaactcc atagatataa tcagcagctt
    41521 ccatagttcc tctcctatga gttactgata taaattgagt ctctttagat aaatccttca
    41581 aaaattctcc aaatctaaat atatttgcat catcaagtgg cgcttcaatc tcatctaata
    41641 tacaaaatgg tgttggcttt gtaatcaata tagcaaataa tatacttatt gctgttaaag
    41701 ctttctctcc accagataat agatttaaat ttttcatttt tttccctgga ggttgagctg
    41761 ttattagtat atcactttct aataaatttt ctttatctaa aatagttaat tctccacaac
    41821 ctccaccaaa taatctctta tatacatatt taaaattttt acttatttct tcaaacttaa
    41881 tttcaaattc agatttcata ttttcttcta aagtgtgtat aagtttttct atctcttcca
    41941 tagactcttc taaatcttgc ttttgttcgc tataaaaatc gtatctttct tttatttcct
    42001 catattcctt tatagagcct atatttatat ttccaagact tcttatttct ctctttagac
    42061 tttctaagaa ttttctatct atatccaaat cttcatcttt aatttccaat gcttgtacta
    42121 aagtcatatc atattgctca aacaacttat ttatatatgt gtcttgacta gtttttaatc
    42181 tttctaatct tccaccaact ttaaataaac tttctttaag ctctatatgc tgtctatcta
    42241 tagtttttaa ttctttatta atttcatcaa aactattttt taaatcatct tttgctattt
    42301 ttctagtttc aagatttcta ttactatccc ctagttgttt tgttaaattt tctttttctt
    42361 tttcttcagt ggctatagaa tcttgtaatt taattatttc atgttcttca taatttaatg
    42421 actcttctaa ttgtttattc ttttcttcta gctcacaatt ttcaccagat attcttttta
    42481 tatctctaac tatactatta taagcttctg ttttcttcac taaacttaag ttcaattcat
    42541 caaattcaga tttttcttta tcatataaat cattgtatct ttttatttcc tcattcaatg
    42601 catcaatttt ttctttattt ttattgtata aatcatctaa ctcttccata tcttttctaa
    42661 catcatcgct tttttcaagt gtataattta gattagaatt taaatcattc ttctcatttt
    42721 ctaatttagt aatactacct tttaacgatt caatttcaga ttcaacattc tttatacttg
    42781 tactttttat tattatactt ttttctaaat cttttatttt actttcatgt gaatcaattt
    42841 catttttgat atttttcaca tctttgtcta aagattctct ttttaattct agatgtgaaa
    42901 tttcattctt gatattactt atcttttctg tatattcatt aatgtatctt tttctcgata
    42961 gtatattccc attagttttt agacttcccc cagtcaaaga acctcctgga tttaagattt
    43021 caccatctaa agttactatt ttaaacctat gaccagtttc ttttgcaaat tttatgccct
    43081 catctatatt atttataagt attgttcttc caagtatatt ttcaattata tttctatact
    43141 tttcatcaaa tgtaattaaa tcacttgcta ttcctatgaa cttagtattt gcttttaaat
    43201 ttcctagact tactttattt gatttaatta tatttaatgg aagaaatgtt actctaccta
    43261 aattattctg ttttagatag tttattgcag acttagcact aaactcatta tcagttatta
    43321 tattttgcat ataagcacca agagctgctt ctatagattt ctcatatttt tcaggtacat
    43381 ttataatttg tcctaatgct ccatgaactc cttttaaatt tttatttttt agtacttctt
    43441 ttactcctct attaaatcct tcataatgat tttccatatc tatatagaca tttaactttg
    43501 aattatatcc atttaaatta tatttacttt tctgtatttc atcttctagt ttattgtgtt
    43561 cactaattga atcctgcaaa ttaattgata attctttatg tctattattt acattcttta
    43621 aattttcatt ttgcatatta aattctttat ttatagtatc taattcagat gattttattt
    43681 caatattttt gtttaactct gttatctctg aatttatatt ttcatctctg atattcatat
//
LOCUS       DAEPOG010000011        42866 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEPOG010000011 DAEPOG010000000
VERSION     DAEPOG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.011"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..285
                     /locus_tag="KR432_000528"
     CDS             <1..285
                     /locus_tag="KR432_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBH0639083.1"
                     /translation="KNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRNDY
                     WIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(396..656)
                     /locus_tag="KR432_000529"
     CDS             complement(396..656)
                     /locus_tag="KR432_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBH0639084.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            873..2156
                     /gene="hflX"
                     /locus_tag="KR432_000530"
     CDS             873..2156
                     /gene="hflX"
                     /locus_tag="KR432_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0639085.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            2164..2841
                     /locus_tag="KR432_000531"
     CDS             2164..2841
                     /locus_tag="KR432_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH0639086.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2927..3370
                     /locus_tag="KR432_000532"
     CDS             2927..3370
                     /locus_tag="KR432_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0639087.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3676..4320
                     /locus_tag="KR432_000533"
     CDS             3676..4320
                     /locus_tag="KR432_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBH0639088.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(4406..4783)
                     /locus_tag="KR432_000534"
     CDS             complement(4406..4783)
                     /locus_tag="KR432_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0639089.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(4783..5169)
                     /locus_tag="KR432_000535"
     CDS             complement(4783..5169)
                     /locus_tag="KR432_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0639090.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            5348..6103
                     /gene="nadE"
                     /locus_tag="KR432_000536"
     CDS             5348..6103
                     /gene="nadE"
                     /locus_tag="KR432_000536"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBH0639091.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            6186..6905
                     /locus_tag="KR432_000537"
     CDS             6186..6905
                     /locus_tag="KR432_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0639092.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(7073..8077)
                     /locus_tag="KR432_000538"
     CDS             complement(7073..8077)
                     /locus_tag="KR432_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0639093.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            8343..9239
                     /locus_tag="KR432_000539"
     CDS             8343..9239
                     /locus_tag="KR432_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBH0639094.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            9377..10639
                     /locus_tag="KR432_000540"
     CDS             9377..10639
                     /locus_tag="KR432_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639095.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            10715..11908
                     /locus_tag="KR432_000541"
     CDS             10715..11908
                     /locus_tag="KR432_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0639096.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            12537..13331
                     /locus_tag="KR432_000542"
     CDS             12537..13331
                     /locus_tag="KR432_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0639097.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            13425..14765
                     /locus_tag="KR432_000543"
     CDS             13425..14765
                     /locus_tag="KR432_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0639098.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            14902..16134
                     /locus_tag="KR432_000544"
     CDS             14902..16134
                     /locus_tag="KR432_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0639099.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            16174..17316
                     /locus_tag="KR432_000545"
     CDS             16174..17316
                     /locus_tag="KR432_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0639100.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            17327..18121
                     /locus_tag="KR432_000546"
     CDS             17327..18121
                     /locus_tag="KR432_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0639101.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            18132..19154
                     /locus_tag="KR432_000547"
     CDS             18132..19154
                     /locus_tag="KR432_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0639102.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            19253..20629
                     /locus_tag="KR432_000548"
     CDS             19253..20629
                     /locus_tag="KR432_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0639103.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(20961..21305)
                     /locus_tag="KR432_000549"
     CDS             complement(20961..21305)
                     /locus_tag="KR432_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639104.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            21793..22803
                     /locus_tag="KR432_000550"
     CDS             21793..22803
                     /locus_tag="KR432_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0639105.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            22939..23427
                     /locus_tag="KR432_000551"
     CDS             22939..23427
                     /locus_tag="KR432_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0639106.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            23603..25531
                     /locus_tag="KR432_000552"
     CDS             23603..25531
                     /locus_tag="KR432_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0639107.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            25706..26116
                     /locus_tag="KR432_000553"
     CDS             25706..26116
                     /locus_tag="KR432_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBH0639108.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            26138..26440
                     /locus_tag="KR432_000554"
     CDS             26138..26440
                     /locus_tag="KR432_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBH0639109.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(26656..27348)
                     /locus_tag="KR432_000555"
     CDS             complement(26656..27348)
                     /locus_tag="KR432_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0639110.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(27374..28561)
                     /locus_tag="KR432_000556"
     CDS             complement(27374..28561)
                     /locus_tag="KR432_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639111.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            28897..30774
                     /locus_tag="KR432_000557"
     CDS             28897..30774
                     /locus_tag="KR432_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0639112.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            31086..32492
                     /locus_tag="KR432_000558"
     CDS             31086..32492
                     /locus_tag="KR432_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0639113.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            32520..33368
                     /locus_tag="KR432_000559"
     CDS             32520..33368
                     /locus_tag="KR432_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0639114.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            34029..34721
                     /locus_tag="KR432_000560"
     CDS             34029..34721
                     /locus_tag="KR432_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639115.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            34718..35653
                     /locus_tag="KR432_000561"
     CDS             34718..35653
                     /locus_tag="KR432_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639116.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            35801..36724
                     /locus_tag="KR432_000562"
     CDS             35801..36724
                     /locus_tag="KR432_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639117.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            36708..37478
                     /locus_tag="KR432_000563"
     CDS             36708..37478
                     /locus_tag="KR432_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639118.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            37488..38270
                     /locus_tag="KR432_000564"
     CDS             37488..38270
                     /locus_tag="KR432_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639119.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            38647..39183
                     /locus_tag="KR432_000565"
     CDS             38647..39183
                     /locus_tag="KR432_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0639120.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            39215..39604
                     /locus_tag="KR432_000566"
     CDS             39215..39604
                     /locus_tag="KR432_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0639121.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            39641..40027
                     /locus_tag="KR432_000567"
     CDS             39641..40027
                     /locus_tag="KR432_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBH0639122.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            40154..41596
                     /locus_tag="KR432_000568"
     CDS             40154..41596
                     /locus_tag="KR432_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0639123.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            41893..42708
                     /locus_tag="KR432_000569"
     CDS             41893..42708
                     /locus_tag="KR432_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0639124.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        15972 a         5043 c         8008 g        13843 t
ORIGIN      
        1 aaaaatataa aaatcttgaa aactacatca ataacactta taattatgcc attattatta
       61 acagttccaa ttttattaaa ttttgaaaaa tcatttatta tttttcataa attattattt
      121 agaaatgatt attggatatt taatccaaat ttagacccag ttataaatat attgccagag
      181 gaattttttt ttcactcagg aatgatgatt ttaatattaa ttttgttagc aagtatctta
      241 ctatttgtaa tgtatagatt atataaatct ttcaaaataa aataagatag caaaaacgaa
      301 ttatattaaa ggttatacaa taaaaatagc tatgtaataa tatttaaact aagagtagtc
      361 gataaatatt tatgcatagc tatttctaat agaaattact ttttactagt gattttaact
      421 aaatcttgaa atttgcccat aatgtaattg taattatctt ttttgtgagg taacatgcaa
      481 ctactgcaat ctttgatacc tttatctgta tacttaaagt ttccaccaca gttttctcct
      541 aaagcataca aaggacaata gcaaaataga cagttaaact cttctggttt attagtttta
      601 tgacaaggga aaaattcgca atctttgtga ttaaaaaact tataattttc gcccatattt
      661 tcctcctaat taaataaaat ttaaattttt tatactatat aagattttgg tgataatata
      721 attatactaa ataattttat gaatagtcga taatatagta ttattaatca tagtattaat
      781 ggtataatta gtcaacagaa aaataatgta aaaattatgt aagtgtattt tgtaattaga
      841 atatgtttta taacaaaaaa tgaggtgatt aagtgaaaga aattcaagaa aaagcacttc
      901 ttgtaggtct gaatttgact acaatagtga aaaaaaatga tgatatagac actagtgaat
      961 ctatggaaga attaaaagag ctaacaaaag ctgctggggc agaagttgta ggtagcttaa
     1021 ttcaaaataa acattctgta gatgcagctt attatatagg aaaaggtaaa gtagaagaaa
     1081 taagagccta tagtgactct ttagatgcta ctttagtaat ttttaatgat gaattatctg
     1141 gtgctcaaat cagaaatata gagaatgttg ttggaagaaa agtaatagat agaactactt
     1201 taattttaga tatatttgct caaagagcac ttagtaaaga aggtaaatta caagttgaat
     1261 tagcacaatt aaaatataga ttgccacgac tttatggaat gggtggtgag atgagtagaa
     1321 caggtgcagg gattggtact agaggtcctg gagaacaaaa actagaaata gataagagac
     1381 atatacttaa taaagcagca gacataaggc gtgaacttaa agaagtaaaa aagaataggg
     1441 agacacagag agttaagcgt ttaaagtcaa atataccgat tgtagcactt gttgggtata
     1501 caaatgcagg aaaatctact cttttgaacg aattaataaa gacacacaag gattatgaac
     1561 aagaaaaaga agtttttgta aaagatatgc tttttgcaac tttggatgta actctaagaa
     1621 aggctctttt gccgaataaa aaagaatttt tagtagtaga tactgttgga tttgtaagta
     1681 aacttcccca tgatttagta gaggcgttta aagcaacatt ggaagaagta cagtatgcag
     1741 atttaatttt acatgtaatt gatgcgacaa atactagtta tgaattgcaa aaaagtacga
     1801 ctgaaggtgt attaaaagaa cttggtgtaa atgataaaaa acatatactt gtatataata
     1861 aagtggataa gctagaattg gatatatatc ctaaaagtca agaagatatt gtgtatattt
     1921 ctgcaaaaca aggcataaat atggataagc tattaaatat gatagaaatt gctttaatgg
     1981 aaaatactta ttcagtaagc ttgatgttac cttatgagag aggtgatata tttagtagaa
     2041 taaaagataa atataatgtg gagaattttg agtatgggga aaatggcata acgctagatg
     2101 taaacttcga tgaagaagat tttaatatct atagagagta catactagaa aaataggttg
     2161 aatatggata taaattggga aaatatagat aatcttgaag attattttat aacctatctt
     2221 ctttataaag aatctaaaac agtatctcaa attagtaaaa ttagaaatat ttcaagtact
     2281 gaagcaggtg agcaacttat acaagcaaaa ttaaaaataa aggaaatgca aaaagatgat
     2341 tttgaggcat ctaaggatat tttagataaa tttttagaac tagataaaat taagagactt
     2401 gattttatgg atagcttaga tgacgaaaaa atggtatact ttaagagaaa ggttttcaaa
     2461 agaatccttg tggaaaaaaa tgctgaagat ttaattgttt taatttgggc gacaggtgag
     2521 ctaaaagatg atagattctt aaagttgctt catcagctca caaaccacag acactcagat
     2581 attagaagaa taacttattc agcaatcaga aagattgaat caccaagtag tagagaagta
     2641 ctgcaaaaag gtctctacga taaaaatgca caaactagac agtattgtgc aaaggctctt
     2701 tcaaaactag gggatgaaaa cagtttaaag attttacagc agttgaaaga caagaataag
     2761 aattttgaaa aagaatatgt gcttagagct tatgacgaag ccattaagtc attggaaaat
     2821 gcaaaaaatt tgaaaaagta attttttatt aaagcaattt gtttacttaa tgatgtattt
     2881 ctagagttta acaaggtaat aaataaatta atgggagggg tttcaaatga tagtaagacg
     2941 ttgtgcaggt ggagtagtgt tttatgctaa taaagtactt attgtaaaaa atgatagggg
     3001 agaatggaca cttccaaaag gaaagatact tggaggagga cttccatatg agagtgctgt
     3061 tcaaagagtt aaagtagaaa ctggcataga tgctaaaatg atagatgtgg ctggcgatac
     3121 tatgtatgaa tttttctcaa gaagtagaca acaagaagtt tgtaatgcaa taatgtggta
     3181 tgttatggaa gcttgtaaca ctaattgtgt acttgctcct gaatttcaag aaggtggatt
     3241 ttataaagta aaggatgctt tagatatgtt atcacatcat aaagagcaag ctttagtaga
     3301 agtatcttat aaaaagttta aggaattaaa gaaattagtt tcagagggcg atgatgttcc
     3361 agcaaattaa ttgtgaattt agaggattct gaggaaggga tagccccttc cttttttaat
     3421 tgaagcagtt ttaagcgtaa attatatagt ctctttaaat tatgatttat caatttagag
     3481 ctttagtagt ttatatttaa aatcagtaag atatatttaa aataaaaatt aaaaagtata
     3541 caattttatt atataattgt cttatgatat tgattaaaga agtattaaaa gtccatttat
     3601 ttttaaatag aataatttta tagatatata atttgatgtg tatattagaa aatgattaaa
     3661 ataggaggaa atacaatgag caattacagg acattacatg aatttggaac agatgaaata
     3721 actattgaaa agtctgtttt tattggatat gctaagccta taaagagtga agaagaagca
     3781 ttagagttta taaatgaaat aaagaaaaag cataaagatg caactcacaa tgtgtgggct
     3841 tatactgtag gtaaaaatat gaatatacaa agatatagtg atgatggaga accacaagga
     3901 acagctggaa tacctacatt agaggttata aaaaaagaag atttgagaga tgtagtggtt
     3961 gttgttacta ggtattttgg aggaacaaaa ttaggtgcag gcggtctagt tagagcttat
     4021 acaaaaggtg ctaaattggg acttgaagca ggtaagataa tttataaggt aatgtatcaa
     4081 gaagttaagg ttaagataga ctatactcaa cttggaaaag tgcaaaatga gttaatgaat
     4141 ttggggtatt ttataaaaga tactgtatat gaggataatg ttgagatagt agtgtattca
     4201 agattagaag atgtagagaa attatcagaa aaaatgattg atataacaaa tggaacagga
     4261 aaaataattt taggagagga attttatttg tctgaacaaa atggagaaat tttaatataa
     4321 tctaaaaggt tattagaata gttaatacct caaatagcca attagtattt ttaagtgaaa
     4381 agccattaaa ttgatttaat ctaaatcaat ttaatggctt tttatcggat tttgcacaga
     4441 atcctttttt tacttcaaat aaaccgacta aaagtaaaaa tatcccaaat atttttttta
     4501 atatatctga agacagataa ttagctaata gtgaaccaaa tacagctcct aaaaccccaa
     4561 acacagctat aggtataacc aatttaaaaa ttacattttt attttttata tgtatagcaa
     4621 gtgctataag tgtcataggg atagatgaaa gtaaatttac gctttgtgct attttagtgt
     4681 ctatgccagc aagtaaaacg agtgcaggta taaggattgt tcctccaccc attcccattc
     4741 ctccaataat tcctgcaaaa aatccaatta gagcaaataa cattaaaaca ccatccttaa
     4801 tgctgcaatt atcataatta ttccaaaaga aattctaaga aactttccag taaccttatt
     4861 tagtaattta gctcctataa taccaccaag aatacttcca actgcaactt taattgttaa
     4921 gttaacatca taagtacctt tagatacata taaaacagaa cttgctgtcg ttaagaatat
     4981 tattatagaa agagcagttg catgagattt atgttcttct accttaacga tgtcatttaa
     5041 tattggaact aaaagagtac ctcctccaga accaaaaact ccatttataa atcctgtaaa
     5101 aactccaatt atcgtatttt ttaagttaaa atcaaagtat tttttcttta aatcatcagt
     5161 ttttttcata aaaatcacct tctgaaatag ttttaccaaa ataggcaaat tatatttata
     5221 taaaataaga ttaaatataa tctttcatgt ttgtttaatt ttgcataaaa aaaaacatat
     5281 taaaacaaca tattaatata tggtataata tattaaaaca aagtagcaaa tttgagagga
     5341 gataacaatg tcaaatataa aaatacaaat agacaaaact gtagaatggc ttataaataa
     5401 ggttaatgag gcaaatgcaa aaggattaat tgttggtgta tctggaggaa ttgattcggc
     5461 agtagttgct aatttaatta aaaaggcatt tccagaaaac tctatgggag ttataatgag
     5521 cataaaaagt aatcctcaag atagagaaga tgcacttaag gttatagagg gatgtgatat
     5581 agaatacctt gatttagacc ttatagaacc acaaagtgct atattagaca tggtagtagg
     5641 aaatttaaaa gataaacatt tatatagagc agaatattta aagatgacag atgcgaactt
     5701 aagagcaaga gtaagaatga gtacaattta tacaattgct aacaatctag gatatttagt
     5761 tgttggtaca gacaatgctg cagaaataca cacaggatat tttactaaat ttggtgatgg
     5821 tggagttgat attttaccta ttgcaaattt aactaaagga gaagtatatg aatgggcaaa
     5881 agagcttggt gttcatgaag atttaataaa caaagctcct tcagcaggtc tttgggaagg
     5941 tcaaacagat gaagatgaaa tgggaactac ttacaatatg atagatgctg tgttagaagg
     6001 tagattggat gaagttccta aaagagacca agagataata gaaagacttc atagattaag
     6061 tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc taatttacta tttataagat
     6121 ttgtgataaa atttacaagt gatgaaaatt agaataatta attgtacgta ttaaggagtg
     6181 gaaatatggg acgtataggt aatataatta atagaaaagg taaacaagat gctcaaagag
     6241 caaagatatt tactaaacat gctagagcaa tagcagttgc agctaaagaa ggtggagcag
     6301 acccagagta caatgcagcg ttaaaaacag ctatagaaaa agctaaggca gataatatgc
     6361 caaatgataa tatagataga gctatcgcta aaggtgctgg agcaggcgct ggagaagact
     6421 atgaaactat agtttatgaa ggatatggac ctggtggagt tgctgttata gtagaaactt
     6481 taactgacaa caaaaacaga actgctggta acgtaagata ctattttgac aaaaacggag
     6541 gaaacttagg aactagtgga tgtgtgtctt ttatgtttga taaaaaagga caaatattag
     6601 ttggtttagg agatggagtt tcagaagaag aattgatgga tgttgcatta gaagcaggag
     6661 ctgaagattt cataacagaa gaagatggtt atgaaataat aactacacca gaagattttt
     6721 ctagtgttcg tgatgagtta aaagctaaag gatatgagtt tatatctgct gatgtgaaga
     6781 tgatacctca aactacgact gtattgactg aagaatctca tttaaaaatg atgaataaat
     6841 tagttgatat gcttgaagaa gatgatgatg ttcaagatat atatcataac tgggaagtag
     6901 aataaatagt ataagcatat tgaaatttat aagcattgaa tcaattttaa catgtgataa
     6961 attttataga ataagaaatt actaaaatag acatacgcac ctctaaccta tatttgcata
     7021 ataatatgta agtataggta aggggtgttt ttatatttat aggttaagaa atttaaagta
     7081 aattttgagc tggacttgat tgtctataaa taattgatgg gtatataact tgttgctgaa
     7141 taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt tctgctgtct
     7201 cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt agtcgtgaag
     7261 gtccattact tgctgcaaat atttgaatat cctctccaat tacaattcca gattcatgaa
     7321 atgcattcat cataccaaga gctacaacat catgattaca aaaaattact ttttttagtg
     7381 aattttttgc aagcattttt tgagcaatat catatccact atctatttta ttagaacaat
     7441 aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa aaagatttac
     7501 cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata tcatttttac
     7561 cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca atagatacac
     7621 ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt ttatttttta
     7681 ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca taataattat
     7741 ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt atgatgatat
     7801 ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata ttggttgatg
     7861 ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta tgtttaatag
     7921 gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct tgtgtatgta
     7981 gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt gctgaagaaa
     8041 gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc ttaaatagtt
     8101 gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac aatattctac
     8161 aaggtgcata ttttacagga ttagaataaa taaatttaaa aaaacttctt acttatgttg
     8221 acataattta gtttatagga ttataatatg cttaaataag cttattatta actcaaatta
     8281 agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgtaaa gggggaaaag
     8341 gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa aacctgaaag
     8401 agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct tcagggatta
     8461 aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg aaagatgcaa
     8521 aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat gcattagttc
     8581 caaatttata tggtgctaaa gttgcatggg agtgtggtat aagagatata tcctatgtta
     8641 tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa gagtctattg
     8701 atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt ggaatggcta
     8761 ctgtatttgg gtgtcctttt gaaggtttac caaaaattga agatgccata agatttgcta
     8821 aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata gggattgctg
     8881 accctaagca agtaagagaa actattagtg cattagttga agctttacca gagtgcagat
     8941 ggcaggtaca tattcatgat actagaaata tgggaatggt taatactctt gtggctattg
     9001 aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc ccatttgctc
     9061 ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa cgtatggggt
     9121 atgatacagg agttgatttt gttaaacttc ttgaattatc aaagtatcag aagagttttg
     9181 taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt aattgttagt
     9241 tctattgatt aatcgattaa aaaaatatgt tgattaaaaa aatatgctga ttggtaaaat
     9301 agatttattg ataaaaaaga taaattataa aatacattat ctaataaaac aaataaaaaa
     9361 attgaggggg attagtatgg atattatagc tttactttta tttttaggag ttattattat
     9421 tgcatttgta cgtaagaata atgcaggtat tttggctcta gctattggtg tcatagctgt
     9481 tagaattttt ggaatggatg ataaagaact ttatggagct ataagcaact caatgtttac
     9541 tacacttgtt ggtataacac tactctttgc aatagtgaac tcaacaggag cattaaagct
     9601 tgttgcagaa aaaattgtag ctgcttctgg taaacgtgtt tggcttatac ctatagcagt
     9661 ttttatagca ggatttgttg ttgctggtgt tggacctggg gcaattccag cacttgcaat
     9721 tatcccagca atggctgttc ctgtagccat aacggttggt tataatcctg taatgcttgc
     9781 acttataggt gaatgtggat taatggcagg tcgtatgact cctattactc cagaaggtca
     9841 aattataaca accgttgcag aaggggttgg aattgaaaat gttatgccta ctatcttagc
     9901 ctgtcaaact ctttctacaa ttgtattttc aatagtattg tttataattt tcaaaggata
     9961 taaacttaaa aaacctataa atgttataga tagtaagcaa atagaaaaat ttaatataaa
    10021 gcagataatt tctctaataa gcatagtagt tatgattgct tttatcataa tttttgatat
    10081 gaatgttggg cttgcagctt ttgcagtttc agctgtactt atttttttca atattgctga
    10141 tgaaggagaa tgtatcaaat caattccatg gagcacgata gtaatggttc ttggagtagg
    10201 tgcaatgatg acaatcgtag atgcagcagg aggtattgac ttgatgagta atgcattatc
    10261 atcactgatg aatagtaaaa cagctactcc aattatgagt atttcagcag gacttatgtc
    10321 tatggttagt tctgctttag cagtagttta tccaactatg atgcctatgt gtgttgatat
    10381 agctaaggca gttggaggag tagacccact agcacttatt gctgcagtcg gtgttggagg
    10441 ttctcttgct ggtgtaagcc cactttctac tggtggggca ttgattttag cagctatggg
    10501 ttcaagtaag aaagacttta ataaggaaga acaaaataag gtttttgtac agcttttcat
    10561 tatgagtgca gttggcttat tggttattgc agtagtatca atattatcat ttaatgcaat
    10621 aacacattta atgaactaat tataataact gaaaataatt cattaaattt aatttttaat
    10681 attaatcaat ataatataat ttggagggga agttatgaaa aaaggagcat taagcaatat
    10741 aaaggtactt gaccttacaa gagttcttgc aggtccatat tgtacaatga tgttggcgga
    10801 ttttggagct gatgttataa aaatagagat gccaagaaaa ggtgatgata caagaggttt
    10861 tggacctttt gtaaatggag agagtttata ctatgcaaat ataaatagaa ataaaaaagg
    10921 tatcactcta aaccttaaat cagaagaagg aaaaaatata tttttagaaa tggtaaaaca
    10981 tgctgatgtt gtagtagaaa attacagacc aggtgtaatg gaaaagttag gtctgggtta
    11041 tgatgagttg gctaaagtta acaatcaaat aatttatgga gctgtatcag gatttggatg
    11101 ttatggacca tattcagaac gtccaggtta tgatattatt gcacaggcaa tgggtggtct
    11161 tatgagtata acagggcaag aaggaggaga accaacgaga gttggtaatg ctatgggtga
    11221 tgtactaggt ggaatgaatt taactattgg tatactcaca gcactcaacg ctagaaaaat
    11281 aataggaaaa ggacaaagag tagatgtttc cctcgttgat tctgttgtat cgtctctcga
    11341 aactggtata cagagatact ttgattcagg acaacctgaa cttatgggta atagatatgc
    11401 ttcagcctat ccttatgact cttttaaggc aagtgatgga agttttgtaa taggctgtgg
    11461 taatgacaaa ctttttgaaa agttatgtaa aaaagttctt tttagagaag accttttaat
    11521 agatgagaga tttaaaacaa atcttaagcg ttgtgaacat tacaaggctc ttaaaccaga
    11581 aatagaaaaa tggtctgtta agcatacaat aaaagaagca gttgacttaa tatcagaggc
    11641 tggagttcct gctgctccta tctataatct tgattgtgtt acaacagatg aacatatagc
    11701 aaaagcaaga gaaatgattg tagatattag tcatcctatt ataggaaata tgaaagtcaa
    11761 tggaaatcca gttaagttga tgacaacaaa agctaaaatt gatatgccag caccatctct
    11821 aggggaaagt aacgaggaga tttataatgg ttggcttggt tttgatagtg ataaaataaa
    11881 agatttaaaa gaaaatggag ttatttgatt aaaaaatagc taaacacact tgtgacttta
    11941 gtcatgggag gttcagttag aataatatca aaaaatatta aatcaatact gtaaatcaaa
    12001 tatagaattg tcagaagcat ttaagatggg aaaagctact tagacattga gtctttgtgg
    12061 cttttcctat tttcattaag aactttatat tatcagaata gatatgtact aaactaaagt
    12121 attaacaatt taaattaata gaaaataaac tacctaatta tgttgaataa agactacttt
    12181 agtgaaaata aacttataga gatgctattt aaattatttt aatggaaata tcaaaaatga
    12241 gactgcattc tcaaaaatga gactgcgttt tttaagtatt ttctcgaaaa tgagacaaaa
    12301 atattgtttt atgagtttta ttgaagaaaa attcttctgt gaattacaat ttaatcaatt
    12361 ttaaagttga aaattattaa aaatttagtg aaatttatgt atatttcgtt tattttatta
    12421 aaaataacaa ttttttacta aaaataaaga acttcaatgg tgattggcat agtacttgct
    12481 attttatatt aacaagaaat gtagaaaaaa taacaatgta taatgggggg aatatgatgg
    12541 aatctgtaat ggaaaatttg aacaatctta aggtggaggt taaggacaaa gtctgtgtta
    12601 ttactataaa tagacctaaa gctttaaatg ctcttaatag tgacactctt agggaactat
    12661 cacaagttat tgatgtagta tcagaaaatg aggcaatatt aggagttatt attactggag
    12721 aaggtaaagt ttttgtagcc ggtgctgata tacgccagat gcaaaactat aaaagtgagg
    12781 aaggcagaaa atatgcaggt tatgctcagg gaatttttga taaaattgaa gctcttgaga
    12841 aaacagtaat agcagcagtt aatggatatg cattaggtgg tggatgtgaa cttgctatga
    12901 gttgtgatat tagaattgca tcagaaaagg ctatatttgg acaacctgaa gttaatttag
    12961 gagttattcc atgttttggt ggaactcaaa gattatcaag acttgttgga actggaatag
    13021 ctaaagagtt aatttttacg gggagacaag taaatgcaga agaagctaaa tctattgggc
    13081 ttattaataa agtggttcca agtgatttac ttttagaaga gtctatgaag atgatgaacc
    13141 aaatagttga aaaagcgcca atagcaataa gatatgcaaa agttgttatt aacaaaggga
    13201 tagatatgga tttaaagaat gctcttgaac tagagaaaga tattgcaggt ttaacttttg
    13261 ctacaagaga taagcaagaa ggaatgaatg catttataga aaagagaaag ccagtttttg
    13321 aaaataagta gctattataa ctaaattgga acaatgtaga atatattcaa aatctaaatt
    13381 tcgtgaaaaa atacatatag tttaatttta aaaggagtta atttatgaat acgggagtaa
    13441 ttataagttt agtagggatt atcatagctc tatctcttct agttgtaatg gttatgaagg
    13501 gaataaatat ttttattata gcaattgtat gttcatcatt agttgctata acaggaagcc
    13561 taaatgtgta tgatgcactt aaagtggatt atatgacagg atttgttgga tttcttcagg
    13621 caaatttctt tattttccta acagggacat taatgggaaa aataatggag attactggtg
    13681 gagcaaaatc tattgctaag atgattgtaa gatggattgg taaggataaa gcactacttt
    13741 ctattccaat agcttgtgga atattagcct atggtggagt aagtgttttc gttgtaagtt
    13801 ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc tgatttgcca cgaagattta
    13861 taccagcagc tttgactttt ggatgctcaa cttttgctat ggttgcacca ggggcaccac
    13921 aaattcagaa tattgttcca gcatcaacat tagggacaga tattatggcc ggagcagtaa
    13981 atggttttat atcatgtgca gtaatgttta tagttggaag taccattttg tacaaaatgg
    14041 tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca tgaatcagat atatttgatg
    14101 aagcagaagc aactaattct aagagagaaa atggtcctaa tggtgtaatt gccttaattc
    14161 cattgatagt gtcaatttta ataattaatg taaaagttaa tggaaaagcg atagtaccaa
    14221 ttgaggtggg agtatttatt ggagctttat tagtatattt attgttaaat aaatatcaag
    14281 ataatactaa aatagttgga catgttggtg atgcatgtaa aactacagta gtagcaatat
    14341 gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa atcagcagtt ggttttgact
    14401 tcatagttaa tgccatgact aatattcctg gtcctcctat tgcaggggct gcagttggaa
    14461 ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg actaggaatt gcagctccac
    14521 ttcttggacc agtattttta gccaagggtg tttccgctgc agcattaact aggactatgg
    14581 caatttcatc agcagcatta gattcacttc cacacaatgg atatattgtt actgtaacaa
    14641 atggtctgtg taaagaaaca cataaggatg cctatatgcc aatattctgg gtaactgttt
    14701 taactccgtt tattggaacc atagtagcag tcctactgtt tacaatgttc ccgggattgc
    14761 cataagagaa aagataaaag gtataaatta tattttgata ttataaaatc aattttcaaa
    14821 atatatacaa taaatttcat gttttaatac tataaatatg tatttaaaat aacaaattga
    14881 ccatataagg aggaattgaa agtgttaaat aaaccattag agggagttaa ggttattgac
    14941 ctaacgtatt ttgtagcagg tccagggact tctaaaatat tagctgattg gggtgctgac
    15001 gttattaagg tagagccaag ctttggagac ccaggaagaa agacaggggc aacaatgaca
    15061 atgccaatag atgattataa taatcctttt tacagcacat ataattcaaa taaaagaggt
    15121 ttgtctataa atcttaagag tgaaactgga attgaaatta tggataagct tttaagtgaa
    15181 gcaaacgtat ttgtttcaag ttatagaaca ggagcattaa aaagactagg tcttgattat
    15241 gaatcattaa gtaaaaagca tccacacctt atctggggtc agataaatgg ttttggcgat
    15301 tttggacctg ctaaggataa tgcaggattt gatacagttg ccttttgggc aagaagtggg
    15361 gctatgcttg atatagcaga aaaagataca agtccaataa atccacctat agcctttgga
    15421 gatgcaacaa cttcttgtag tttatctgga ggaatatgtg cagctttgta ccagcaagct
    15481 aagactggaa aaggacaaaa agtaatggca tctttatttg ggcaagcaat atggaatgca
    15541 tcatctttat tagcatctac acagtttagt gatgagtatc caaaaactag aaaaaatgct
    15601 atttcaccag taataaattc ttatcgttgt aaggatggaa agtggatatt cttaagtatt
    15661 ttagagcatg aaagatattt tgaggcactt tgtagattat ttggaagaga agacttaata
    15721 aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa atgctacaga acttattaat
    15781 attttggatg gtgaatttgc aaagtttact caaaatgaga tggttgaaaa acttactgct
    15841 gctgatatag ctcatgaaaa aattcaacat gttaatgatg tagtgactga tgaacaggct
    15901 attgctaata actatatata tgagcataca agtaaaaatg gaaataagac aatgcttgca
    15961 tccactccag ttaaatttgg aaatattgaa gttaacatga cttgtgatgc tccacttata
    16021 ggagaacatt cagatgagat attgagagaa cttggataca gtgattctga tatagaagct
    16081 ctaattgaat ctggaattgt aacaatgaat aaagaagcat gtgaaatatg ttaataaaca
    16141 tgtgaagtat gttaataaat aggagggaat tttatggatt ttagattaac agaagcacaa
    16201 ttaatgcttc aaagagtagc aaaagaattt gcagagaatg aaatagcacc aatagctgct
    16261 gaaacagata aaacaggtat ttttccaagg gagcttttta gcaaaatggc taaaatagga
    16321 tttaatggca ttggaacacc tgtagagtat agtggttctg gagggacaga tatagaaaag
    16381 gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat ctgcagcaat attatcaata
    16441 cacacaatat atgctcaagc tattttgaaa tttggtactg aggaacaaaa gaaaaaatac
    16501 cttccaatga tggctgaagg tgggtgtgta ggtgctttcg ccttaactga acctaatgct
    16561 ggttcagatg ctgcaagagc tgcaacaact gcaattattg atgaagaaac ggatgaatat
    16621 gttttaaatg gaactaagtg ttttatttct ggtggaggtc aagcagaatc attaataata
    16681 tttgcactga cagacccatc aaagggaata aagggaatgt ctgctatcat tgtcgacaaa
    16741 ggtactccag gtttttcaat aggaaaaatt gaagaaaaaa tgggtattca tggttcagaa
    16801 actgcagagt taatatttga taactgtaga gttccaaagt ctaacttatt aggaaaagaa
    16861 ggcaaaggat ttaatatagc tatgacttgt ttagatggag caagaattgg aattggagct
    16921 caagctgtag gaattgctga aggggcttta gaagaaagta tcaaatattc taaagaaaga
    16981 gtccagtttg gcaagccaat ttcagcgttg caaggtattc aatggtatat agcagatatg
    17041 gcgacaatgg tggaatcagc aaaattatta gtatattatg ctgcagattt gaaagctaga
    17101 ggagaaaaac acactaaaga agctgctatg gcaaaataca atgcgtcaag tactgcaaga
    17161 gaggttacaa atctagccct tcaaatccat ggtggatatg gatatatgaa ggattatcca
    17221 ttagagagaa tgtacagaga tgccaaaata acagaaattt acgaaggtac ttcagaaata
    17281 cacaaagttg ttatttcaag agcagtacta ggataggagg aaaagaatga atatagttgt
    17341 ttgcttaaag caagttccag atactaatga agttaaaata aataaagaga caggtactct
    17401 aataagagat ggtgtaccaa gtataatcaa tccagatgat agaaatgctt tagaagaagc
    17461 tctaaaaatg aaagatgaac taggagcagt gattaaggtt ataagcatgg gacctcctca
    17521 agctaaaagt gctttaaaag aagctttggc aatgggagct gatgaagctt atttaatctc
    17581 agatagagct tttggtggtt cagatacttg ggctacatct acaattattg ctgctgctat
    17641 agaaaaggtt ggaaagtatg atgtaatatt ctgcggaaga caagctatag atggagatac
    17701 agctcaggtt ggaccagagg ttgcagaatt tttagggata ccacaagtta cttatgcaaa
    17761 agaagtaaag gttcaagatg acaaattact agtaactcgt tatacagaga caggggatta
    17821 tttaatagaa gcaaagatgc cagttttatt aactgctata aaagagttaa acaatccaag
    17881 atatccaagt gtaaaaggaa tcttagaagc ttataataat ggagatgcga agattactgt
    17941 attaacactt gcagatttag atgttgatac aactcaaatt ggattaaagg gttcacctac
    18001 aaatgtttat aagtcttttg caccagttaa agacaagcat aatgaaataa tagaaggtat
    18061 aaataaaaaa gaaaaagctg agaaactaat agaaatacta ttcgatttga aactagtata
    18121 ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat aaatttaaat gattataatg
    18181 gtgtttgggt aataggggag caaagggaag gcaaaattaa tcctgttacg atagaactta
    18241 ttggtgaagg aagaaagctt gctgaccaat taggaaaaga gcttgaagtt gtgatagctg
    18301 gatatgaagt agagaaagaa gtgaaggaat tacttcacta tagtgttgat aaagtatatt
    18361 acatcaatga ccctcttctt aaagatttca ctactgatgg atatgcaatt tcaattgcaa
    18421 atttaatcga aagaaaaaag ccagaggttg tattggttgg tgctacttct ataggaagag
    18481 atatagcgcc tagaattgca ggtaaggttg gaacaggtct aactgctgac tgtacaaaac
    18541 ttgaaataga ttcaacagat aataagttat tacaaacaag accagcattt ggtggaaatt
    18601 taatggccac tattgtttgt ccaaaaaata gaccacagat gtcaacagtt cgtccaggag
    18661 ttatggcaaa agctgtaaga aacgaatcag agactggaat tttagaagtt gttactccag
    18721 aacttactga aaaaatgatt agaactagat tagtagaggt acttccacaa gaaaagaaat
    18781 ctgtaaatct aactgatgct agaataatag tatcaggagg aagaggatta aaaagagcag
    18841 aaggatttga gcttataaaa gaattggctg ataaattggg ggcagagatt ggtgcttcaa
    18901 gagcagcagt agattcaggg tggatagaac attctcatca agtgggacaa acaggaacta
    18961 ctgttagacc agaactatac attgcatgtg gaatatctgg agcaatacaa cacttagcag
    19021 gtatgagtga ttctaaatat atagttgcta taaataaaga tgcaaaagcc cctattttta
    19081 gtatatgtga ctatggaata gtaggagatt tatatgaaat tatacctgaa atgatagaat
    19141 cattaaatag gtaactttta gaaaacattg aatatgtaga atgataagtc ttataatatt
    19201 gtattgtaag acttattatt tttgctgaag gttatagtgg ggagatgaaa caatgaaaga
    19261 gcagtggtat aaggatatat ttgcaagaat attatctatg actgatgatg gttttattgt
    19321 tgtaaataca agtggagtaa taatagatat taatgataaa tattgtgact ttttaggaaa
    19381 agaaagaaaa gatataatag gacaaaatat acaaagtatt ataccaaata caaaaatgct
    19441 agatgttatg aaagataagt actgtgaaga aggagctata catcattatt caggtggaaa
    19501 tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgta gaaaatgaca atggagaagt
    19561 agttgcagga gtagctcaag ttaaatttag attgcaatca tttgatgtag ctaaaaaatt
    19621 aatgtcagaa tatatggagc tccaatatta taaagagcag tttaaggata actgcggttt
    19681 tgacaagctc ataggtgaaa atagagactt catagaactt aagaaaacag gtgtgaaagc
    19741 ttctaaaaca aactttccag tacttttaac gggtgaaaca ggcacaggta aagaagtctt
    19801 tgctagagca attcacaata atagcagtag gtcagataaa cctatggtta gtataaactg
    19861 cgctgcaata ccagaagaat tattagagtc agagctattt ggttatgacg aaggagcttt
    19921 tacaggagca aaaaagggtg gtaaaaaagg taagttttta gttgcaaata atggtacaat
    19981 ctttttagac gagataggtg acatgccttt gaccatgcaa gcaaaacttt taagggttct
    20041 tcaggaaagt gagatagaac cagtgggagg tcttaaaact ataaagatag atgtaagagt
    20101 aatctctgcc acaagaaaga atttaagtaa aatggttgaa gagggattat ttagagaaga
    20161 cctatactat agattaaatg taataaacat tcatatgatg gaattgaagg acaggcaaga
    20221 tgatatttta ttgctagcaa attatatatt aaacaaatta aatgttgaat acaaggaatt
    20281 gaaggtttta agtgataagg ttaaaaattg ttttattaac tatacttggc cgggaaatat
    20341 aagagaacta caaaatgtaa taaaaagtgc ctatgctgta agtgatgata tggtcattat
    20401 gatgtgtgac cttccatcta agatggataa tatcagcaga gtagctcaat gtaatgtgga
    20461 tagcaattgt tcaattcatg aaatggttga gaattatgaa aaaagcttaa ttattgatgt
    20521 gttaaggaag tataattgga aatgttctaa ggcagcagag gttatgggaa tacacaagag
    20581 tcttttatat aagaaaataa aaaagtatga aattgaatta aataattaag ataacttgaa
    20641 ttaaggcaat gctaaaaaaa gcgcccttat tcaaagcgaa ctgtataaac tgttttatgc
    20701 agttaaaacg tgtaaaagta gatgtgaagt actaatgtca atcattagta ctttttattg
    20761 tatttataat tactatctat atttcagtat cttttgatat ctaagattat tatgtttaat
    20821 ttaggatata acaaaaccct cttagttatt gacagttttt tatattttaa ctgtctacta
    20881 agaggggata gtttagttta aatttagagt aaattttatc tagcatttat aaaaaatatt
    20941 ttgaatagct tttttgttat ttagtcataa ttttctactt ctttatcaag ttctttgcca
    21001 caagaatcac acttatatat agcattgtat ccataatcat attcatcaac tattttcttt
    21061 tgtctaaata caccaccaca ctcacatagg aaaccaattt ttataagtgt aacgataaat
    21121 tgtgatttta taaattttat atcattacat ttatcgttag tgtaaaatgt ataaatataa
    21181 gagttatcgc actcatctct taattcaaat ctttcaatat atgtgttatt ttttaattcc
    21241 tcaaattctt ctttagttaa atatgatttt tgttgcaatt catgtaaacg cttaatttgg
    21301 tccattattt atatcctccc aataatttta gtattttaag cttagataag gaatctaagt
    21361 tttattaaat ttatcccaag gaattattta aatcatattt agttttatta attacaacat
    21421 atataaatta cacgaaaata aaactaattg taaaaataat atcctgtgaa aacctaaact
    21481 actaacccct tactaacccg aaaactaaaa aaagaaaata tatccaaata taagtgatat
    21541 ataaataaag atgttttata taaatttaac tttaaatatt ctgacaataa tatatataaa
    21601 tatattatag ttaaaatata ccacatattt tgggcattgt atactggaat tagagtaatt
    21661 aatttatttt ttaacataca aatgatttta tgaaaaaaaa caagactcaa aaatttttat
    21721 attctttgat agaataaaat tgagagtaaa tttattgaat tttataaaat ttataagggg
    21781 tgagtctaag atatgcatga agataaaaat gatatatttg aatcaataga tactgaggat
    21841 tttgataata aaacagaaga agaatgttta gagctagtaa gaaaaaatgg attgaattta
    21901 atatacataa aaaatcaaac agaagagata tgtctagagg ctgttagaca aaattgtaat
    21961 gcaataaaat gtgttcagaa tcagacagaa aaaatatgta tagaagcagt taaacaagat
    22021 tggagaatgt tagaatttgt ggaagagtca acagaagaaa tttgcatgtt ggctattaac
    22081 caagatggaa cagcattaaa atatatagaa aatcagacag aggagttatg cttaagagcc
    22141 gttgaaaaaa atggtgcggc tttggagtat gtaaaagaac aaacagaaga aatatgtata
    22201 gaagcagtta gaaacagcga atttggattg gcaagagtga aaaatcaaac agaaaaaata
    22261 tgtatggaag cagttaagca ttgtagctat aatctaaaat gggtagaaaa tcaaacagaa
    22321 gaaatatgta tggaagcagt gagacaaaat gggcttgatt tgaaatttgt aaaaaatcaa
    22381 acagaaacaa tctgtttaag agcagtaaga caaaatggaa tggcattaga atttgtaaag
    22441 gaacagactg ttggtatatg cttaaaggca gtaagacaat atggaatggc attaaaattt
    22501 gtaaaagaac aaacagaaga aatttgtaca gaagctataa aacaagataa aagagcactt
    22561 agttttgtaa aaggagataa ggaaaaatac aaggctctat atgataataa tgaaccattt
    22621 gcaaagagat atgttagaaa tgtaatagaa aaagaaaaca gagcattgat taaaaaagga
    22681 gaagaaaatg caaaaatcaa aattgcagga aaactttatg atagaggcat gtcttttgaa
    22741 gatatatcag atatagttga gattgaaata agcaagttaa aagtgagttt aggtgttttt
    22801 taaagtaata aatgtaggtg ttttttatgg gaaatagtta ataaaaaaat aatcgactag
    22861 ctctttgtag gacagcaaaa ttagtgatat aatttgatgt ataataaatt atttttatta
    22921 gataaggggg aattaaagtt gaagattaca gtattatacc aaacaagaag tggaaacaca
    22981 gaaagggtcg ctaagctgat agaagatgga gctaaaaagg tggatggagt agatgtaaag
    23041 cttatgagac ttgataatat tgatttagat tatttaaatg aaagtaaagc agtaatattt
    23101 ggaacaccta cttatttagc aaatacaact tgggaagtaa aaaaatggtt tgatgaagat
    23161 tcaaaaaaag ttaatttagc tggaaaatta ggagctgttt ttgctacatg tgattatata
    23221 tgtggaggtc cagatgttgc gatacttact atagtaggac atttgatggt taaagggatg
    23281 cttgtgtatt ctggaggtgg ttctttagga aagccattta tacatttagg acatgtacat
    23341 tcaattgaag gtccagagtt acaagatgag aaggctataa tatttggtga aagaattgct
    23401 actaaggcaa aagaactatt tgcttaattt ctatatgcat aataatgatt agagtaaatt
    23461 tagggtttta atggagattt gaagtaaaag ttataaaata aaaaattttc cataaaatta
    23521 ctcaagatga attataatat aattataaat aaattatatt attaaaatat ttaaaatcaa
    23581 acgtcaatag cgagggaagt ttatggtatg gcatatagaa tatgagattt tttctgcaat
    23641 tatagttatt tttttaatgg tatacttttt tagaagtaaa tttataccta ctttacaaaa
    23701 caaaatatat tgtgcacttt taatattctc atttttattt atactttcaa atattttagg
    23761 ttcattttgt ctaaataata tagacaaaat tccaattttt attacttttt tattgaatca
    23821 gatatactta ttgttattgc caataccagc agctcttgta tcattttatg ttatggctat
    23881 tatttatcaa gatataaggt atatgaaaaa aagattatta tttttatcta taccacttat
    23941 aatttgtata atcttatcta ttacaaatcc ttttacgcat attctatttg gattgtcaaa
    24001 agaaacaggt tatatacggg gttgtggata tgttgcaact tttgcatctt ttttctttta
    24061 tgcaatatat tcagcatttt tatcttttaa atatagaaaa gcaatgcatg aatcaaaaat
    24121 atgggctatt agaggttttc ttatagtttc aattgtggca attatcttac aggcaatttt
    24181 tttacagtat cttcttacag gaactgcatg tgcatgtagt ttacttttga catatctttc
    24241 tatgcagaat cgaggtctta ttattgatga ccttactggt gtactcaata gacaatcttt
    24301 tattcaagag ctagatttga atatcaatac tgaacaatca ggatttatta ttacaattgc
    24361 gcttgatgat tttaagttta tgaatgaaac ttttggaaca aaaaatggag atattgcact
    24421 aaaagaagtt ggaaaatatt tgattcagat acatgatgta aaccatgtat atagaatagg
    24481 gggagatata ttttctattg ttgtcaatag tcaattaggt atagaaccag atgatattat
    24541 cagaaagata gaagaaagat ttaaaaagcc ttggctagta gaagagataa gttttaattt
    24601 agcaactagt attgctgttg tttattaccc agaaaatgca gaaacgacag aagatgtaat
    24661 tgttgctata gatttctcta tacatgaagc aaaacgttct gagtcaaggc gagttgttta
    24721 tgcagatgcg tctataagtg aaaagataaa gaagaaacat gttattaaaa attgtttaaa
    24781 agaagcatta aaaaatgatg ggtttgaagt gtactaccaa cctatatttt ctaatagcag
    24841 gggcaggttt acttcagcag aggcattact taggctagaa cacaaagagt taggttttat
    24901 acctcctgat gaatttattc caattgcaga acaaacagga ttgattaact caattggatt
    24961 agtagtcttt gagaaggtat gtagatttat agctagtgat gagtttgaaa atttaaaatt
    25021 ggataatata gctgtgaatc tttctgtagt acaatgtatg caaaaaaact tagctgatga
    25081 tttactttat cttatgaata aatataatgt atcaccaact aaatttaaac ttgaaattac
    25141 tgagactgtg gctatgggct cttttaatat aattaaagaa acaatggaaa gattaattga
    25201 tttaggtgtt aagtttgcac ttgatgattt tggaattgct tattctggag ttactaatat
    25261 gctttcttta ccattttcag taattaagtt agataagagt ttgatttggt ctatgaatga
    25321 agatagtagg cataagctta cagttgagac gattatagct cttataaata aattgaatat
    25381 gaaggctgta gcagaaggtg ttgaaaccat taagtgtgct gaagatttaa tagctatgga
    25441 gtgtgaatat ttgcaagggt attatttttc taaaccagtt ccagaagatg tttttaaaaa
    25501 tttgttgaaa gaaaatactt ttacaagtta acacccttta tttttttaat tacaactttg
    25561 ttcattgaca ctgccagcga tatattataa aattaacaca ataataaata ttaaatttat
    25621 tatacatatt attaatatta attaatggag taaagattta ttatatttta tttaattatc
    25681 ctaaagtgag aaaggaagat gtgacatgaa taaaaagaaa atattagtgc caattgatgg
    25741 gacagaaaga agtatgcatt ctttggaatt tataaaagga atatttaaaa aagatgaagt
    25801 tgaagtagaa ataatgaatg taaaagaact agtatttatt gatggcatat ccctggctga
    25861 agagataaaa aattctgaaa atttaggaag aagaatcttg gataaagcag cagagataat
    25921 gggagagtat gatgttaaag tacattttac ttttggatat ccaggagatg agataattag
    25981 aaaagcaaaa gaagataatg tagattttat agttatgaca aaatctacta aaaaaggtct
    26041 tacaagaatg ataggttcag taacagcaag tgtagttaaa caagcaaaat gtatagtgat
    26101 gattgtaccg gagtagaatt agaaaggtat ttgaaatatg aataaaaaga tagtagcagt
    26161 ttgtgaaagt gaaagaagtt taaattctct taagacagct gcaaatgatt taggatttga
    26221 tttagtttat gaaattcaag aaaatgataa tataaaaaat gaactttcta tttatgatat
    26281 agaagatgca tctatagtgt tatttgttac aagtgattct atagaaagca tagagaagat
    26341 tgatagattt atagatagag aattttatga agttgatcca aagtatataa tagaggatgc
    26401 aaaatctata gtaagtgaaa ttttaataga cttaaattaa tcgtgggtgt cgcactaaga
    26461 atagagcgac gcctttttac atattttaca taagaaataa agggctgtct caaactatag
    26521 ataagactct ttttcattgt tgaaatgatg aaaatatatt tgatttcata caaaaaagag
    26581 tgcctcataa ctaaaaagtt cactttgaga cactccattt ttatatgtta gttgcattta
    26641 aggtaaaata gaagtttaat catgttgaaa taactgaatt acatcttcta aatgattgta
    26701 cattgatgta taacctaagt aagagtcttt actatcaaat gccatcatta tcttgttatg
    26761 gtggctaatc atatccttaa gagaatttct atatttaaaa ccgaagtatt ggttagaaga
    26821 aactgtttta ggtattaact cataatattt tataaaaatt ggattatgag aagctaaaat
    26881 tatctctttg tgaaattgta agtcagcatc ataacattct tctatcaaac catgagaaac
    26941 cttatccaat tcctctattg actttaggta agtatatttt attttttgaa gttctttaga
    27001 tgtagcattt aaaattgcta gtttagtagc ttcactttcg ataagttgtc ttgctcttaa
    27061 agattctaca taaaattctt gtatgttatg atttttatag atttcaccat catcaaatag
    27121 gtcaactttt ttaaatcctt caactatagg acttgagtaa ggcacagctc taagtattcc
    27181 ttccttacag agtatattta tggcatttct tacaggtatt ctacttatgt taaataggtc
    27241 agcaatcttt ctctcacttg gtaatttact attaatttta aaagttccat ttagaatgtt
    27301 taatttaata taatcaataa ttttttcagt ggcatttaag cttttcataa aaactcctaa
    27361 acatataatt tttttatgat tgtattatat catagttaag agttaaatct acttttttat
    27421 aatatgtata agacaggata gaggccaaaa tagctataat agccaagata aagtatccat
    27481 attgtattga agtatattcc gatattttac caactatcat tggaaataca gacattccta
    27541 tagcataaat tgcttgaaaa aaacccattg cagtagattt ctgatgtttt ggtacatttt
    27601 tcatagactc gctagtaagg taagaaagga gtatacctgt agatatacct ggtagcactt
    27661 gtagtacaaa tattatatta atatttttta ttgttggaac taaaaagcaa taggctgaaa
    27721 ctaaaataaa aactaaagga atccaaaatt ttggcccata cttagtacaa aatttagtag
    27781 aagcaaagcc agaagaagca acagcacaca ccatgtaaga tatagaagat agacctatga
    27841 gggtagaact agaacctaag tttttaagaa tttgagtagt aaatgacatt gttgtagaca
    27901 tttgaatacc ctgttgaatt aatgccataa aggaaaaaaa tacaagtctt ttatctttgc
    27961 agatagataa atatttttta attgatattt tttcaggtat tttttttgaa ttatgatttt
    28021 ttatatttaa ggctagaatc gttccaataa ctgaagacaa tacactcatg atacataagg
    28081 ctgtcattcc taatttttca taaaataaag tactcataac aaatccaatc aacattccta
    28141 gattgtttac caatattatc gtactcgtag ctttttgttg atgttctgta gaaaaaaagt
    28201 tggtatagtg aaccatgtaa gaaatccaag ttgatgatga tattccagat aaaatgttag
    28261 ctataagaaa accattgcct ccaggagaaa agactcttat aagagatgct aaaccagcaa
    28321 agaaaattcc agacagtata aatattttat gtttatttat agaatcagca aatactccaa
    28381 caggaatcct taataaacat tgtgagattc catatgcacc tattattata ccaatcatat
    28441 ttgtagatac attacatgca cttaaatatg gattttggaa aggtatatat acatattgtg
    28501 aaaaccagaa aaatgtaaca atagaaagta aaagtaattt ttgattagat atttttttca
    28561 tattatcctc catatggtat ttttataatg caaataatat accatttaga ttttttaatc
    28621 aagtactttc tctattaaaa aaaatttaaa tatataatta ttatttacaa aaaaattata
    28681 ctttatagtt gaattaagaa aacgcttatg atactataaa gttaacaaat ttgaatatat
    28741 aacagtagac gtgttactgg taatgcaggc aagatcgaat caatttttat agattatcta
    28801 tagagtacta tgggtataat ctatttattt ggttcgattt ttttgtttaa ttggccataa
    28861 aacaaaaagc ctatagttga aaatggagga ttttttatgg attataaaaa tatagctcaa
    28921 gaaattctac taaatgtagg aggaaaagag aatgtaaatg aagtgactca ttgtatgaca
    28981 agacttagat ttaaagtcaa aagcgcttct aaagtgaata aggataaatt atctaaaact
    29041 gaaggagtaa taactgttgt tgagagtatg ggtcaaatac aagttgtaat tggaaataaa
    29101 gtaaaaacag tatatgatga ggttataaaa attgtacctc aatcaaatga cgtaggtaaa
    29161 aagaatgaaa gtcaagaaaa gcaaagtata attaattcta tattatcagc agtagctggt
    29221 atatttacac ctacaatacc tgctatagca ggggttggta tgattaaagg tattttatca
    29281 gttcttgcta tgtattatat gaataaaaat ggagtagata taaaagaaac gcaaagttat
    29341 attatcttaa atgccatggc cgattctata ttctatttta tgcctattat actaggatat
    29401 actgcggcta aagtatttaa tgcaaataaa ataatatcta tggtattagg agctacactt
    29461 tgttatccaa cttttactgc tttgatggca ggtgaagaat ctgttagatt tttaggattg
    29521 gcagtaacaa aagctacgta tacatcatct gttattccaa taattattgc tatatgggct
    29581 ctttcatacg ttgaaaaagt attagaaaag tatatacctg aggtaattaa aataataatg
    29641 gttccaactt tatcattggt tgtaatgtta cctgcaacat tatttttatt tggaccaatc
    29701 ggaatttata taggaaatgt cataaacttt tcttataagt atatttatga attaagccca
    29761 gcgttgtgtg gagcatttgt aggtgggtta tggtgtgtgt tggttatatt tggtgctcat
    29821 agagcattat taccaatagg tataagtgat gttgctcaaa caggaagaca gaatttattg
    29881 gcatttgcag gagctgcaaa cttttcacaa gcaggggctg ctctaggagt attctttaaa
    29941 actaaaaatc aaggactaaa aacaatttct atgtctgcaa ctataacagc tttatttggt
    30001 ataactgaac ctgcaatata cggtgccaat cttagattaa aaaaacctat ggtatgtgcc
    30061 gtaatatgtg gtgcatttgg aggtgcgata atgggaatgg gtggagctta tggtaatgca
    30121 tttgcaaacc aaggtgtttt aactatacct gtatatgctg aagcaggtgc attaggtttc
    30181 ttaagttatc taggtggttg tgctatagca ttttttggtt cagctatttc aacttatcta
    30241 gttggatttg aagatttgga agagtcttct aataaggaaa attcatttat aaaagtagaa
    30301 acgaaggatg gtgttattga tataacttca cctgtagagg gcgaatgtat agaattgtca
    30361 gaagtaaagg atgatgtatt tgcttcaaaa gctatgggag aaggtatagc tgtgttacct
    30421 acaaaaggag taataacagc ccctactgat tgtgaagtag cttctttatt tccaacatta
    30481 catgcaatag gattaaaatt agataatgga gcagaaatgt taatacatgt tggaatcaat
    30541 acagtagagt taaatggtaa atactttaca aaacatgtta atcaaggaga tttagtgaaa
    30601 aaaggtgata aacttatatc gtttgatata gataaaataa aaaaggctgg ttatgatgta
    30661 actacgccag ttatagtaaa caatacattt gattttgggc aagtagtttc ttgtaaatct
    30721 agttatgttt caaccaatga taatataatt tcattagtat tagctggtaa ttaagttaat
    30781 ttgactagag ataagtaaat tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat
    30841 ctaaaagtaa gtttattgca atgaattgtt aaagaaaata attaatatgg agtaaagcta
    30901 ttgcaatgaa gtaacaaaga aaataattaa tatagagtaa gtttattgta atgaattgtt
    30961 aaagaaaata attaatatag ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa
    31021 tatagagtga acttattgca atgaatcaac aaaaaaaata atcaatataa aaaggagaaa
    31081 tatttatgaa tacaggattt ccaaaagact ttttatgggg agcatcatct tctgcatttc
    31141 aagtggaagg agcttgggat aaagacaata aaggaaaaac cgtagccgac tataattctt
    31201 ttaaaaagtc tcatttacag gcagatacta aagttgcatc agacttttac cataattatg
    31261 aagaagatat tgaattaatg aaagaattag ggatgaaaac atatcgtttt tcaatttcat
    31321 gggcaaggat aataccagat ggtgaaggag agataaacca gaaaggttta gacttttata
    31381 ataagataat agacaaacta atagagtgtg atattgagcc attcgtaact ttataccatt
    31441 ttgaccttcc atttaaatta gtagagaaat ataatggttg ggaaagtaga gaaactgtct
    31501 atgcttttga aagatttgct aaaatatgct ttaagcattt tggagatagg gtaaagtatt
    31561 ggcaacctca taatgaacag aatttaattg ttagagtgga ggagagaatt aatatatatg
    31621 atgaaacaga ttcatggaag atagataaaa tgcgtgctca gatggattat aatctttgct
    31681 tagcacatgc attagcagtc aatgcatgtc atgagatgat aaaagaaagt aaaattggag
    31741 cagcagtatc atcatctgta acatatcctc ttacttctaa gccagaagat gtatatgcag
    31801 ctagaatgaa tgataatttt aaagtgtact atatgcttga tatgcatcat tatggagaat
    31861 atccagggta ttatatgaag tatcttgaaa aaagaaatac agttcctcac atggaagatg
    31921 gggataaaga gatacttaaa aaagctaaaa tggattttat agctgtaaat tattatagga
    31981 caaattgtgc agaagcttta cctgaagatt cacaacatcc atttggatta agagaaggaa
    32041 ctgttgattt tagtatgtat ggactattta aaatgtcaat gaatcctaac ttagaggctt
    32101 ctgagtatgg agcagcaata gacccatcag gtcttagagt tgccttaaat gaatattggc
    32161 aaagatatca cttacctgtt ataataacag aaaatggtct aggagctaaa gatatattag
    32221 aagatggaaa aattcatgat gactatcgta tagactattt aagaagtcat ataaatgcat
    32281 gtaaactagc tattgaagat ggtgtagaga tgattggata ttgtccatgg tcatttacag
    32341 accttttaag ttcttctcaa ggttttaata agcgttatgg acttgtttat ataaatagaa
    32401 ctgaccatga agttttagat ttaaaaagaa tcaagaaaga tagtttttac tggtataaag
    32461 aagttataga gaacaatgga atagtaaaat aaacataaat atatattgag ggttttagta
    32521 tgtacattac aaaggtatta aataattcgt tgctacttgc aaaggatgat aatgatgaag
    32581 aaataatatt gatggggaaa ggcattggcc ataattataa ggccgggtct gagttaaata
    32641 aaaaagatat agaaaagatt tttgttcttc atgatgaaaa tataaaaaag agtattatac
    32701 aattggcaac agaaatagat gcagaatatt ttggtatagc tcaaatgata attgcatatg
    32761 gaatagaaaa atataactta aaacttatga accatatata tttggcctta actgaccaca
    32821 ttgcctttgc agtgagacgc tttaaagaag gtataatgat tgaaaatcac tatttgttcg
    32881 aaatcaagga atttaatcca aaagagtatg atataggaaa atatgctatt actgttttta
    32941 aggaggttct tggtttagac ctaccagaag aagagattgg taatatagca ggccatttta
    33001 taaatgcaca acaagataat ccttatagtg atagaaataa gagaagtgct aaaattgtaa
    33061 atgctatttt gcaaatagtt cattatcact tttctattgt atataataaa gagtcatttt
    33121 attataggag atttgtaatg catctaaagg ccttttcaca aagatttctg gctaatgaac
    33181 catcaagaga aaaaatagat tttatatatg agcaagttca aaaaaattgt aaggaagagt
    33241 atgaatgtgt gaaaaaggta ggagcttata tgtataagga atactccaga gaattaccaa
    33301 gacaagagga gttatatttg atgattcata ttcataagat tttaggagaa ttatgcgaaa
    33361 atagttagaa tatcttaata tgctagtaat attataaaaa attaaaaatt aactacactt
    33421 taaatatgaa caattgcatt acttaagggg gttgtcttaa aattgatttt tttaattaaa
    33481 tttgaagctc aactcctttt ttaaaccttt atatcattac aactttaatg tttatacgcc
    33541 tgtactttag atgtatatct actttttaca tttctcaaca tacaaaccta ccattttttg
    33601 cagtatgtta attgaaataa accattgagt taaccctaaa tttattctgg acttatagga
    33661 atagttccaa agatacttta aaatattttc tggaataatt gcttatttca catatgtttt
    33721 attgtgatgt tttgatttaa taaattcttc aactctgtat atagtaaatg tattatcatg
    33781 tttgttttat tcaagcacac ttataatacg cttgattttt tcacaatgat gaaatatact
    33841 aatgggtgtt ataggtataa gaatcgaatt acacttgaag aagtaagtga taaatgagat
    33901 ataaatcctc atatgataaa ctactatttc attgaggaac atattctaag tgatgtcaag
    33961 atactacaat atgactaaat tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg
    34021 gaactaagat gaataaagtt ttaattatag atgatgataa agatctttgt attttgttaa
    34081 aaaagagtat ttcaattgaa cagattgaag caaactattg ccattctggg agagatggac
    34141 tggttgaact ttcaaaacaa gattatcagt tagtagttct agacgtgatg atgccaggta
    34201 tggatggttt tgaaactatg gaacaaatac gaaaatatag cagtattccc atcttaatgc
    34261 tgacatcaaa aagcgataat tacagcaaag tgcatggatt gcgagctggt gcagatgact
    34321 atctaaccaa accatttgaa attacagaat ttactgctcg tgtactgtct ttaatccgac
    34381 gttacacccg ttttaatcca aaaaatgagg aaccacagca actttctttc aaaggtttgt
    34441 tgattgacct tgaccagcat acagtgacta ccaatggcga acctatgttg cttccaccca
    34501 aggaatttgg tgtgcttctt tactgtgcaa ggcatcaggg taagattttg acaaaacagc
    34561 agatttatga agctgtgtgg ggtgagccat atgtatatga tgacagcaat atcatggcta
    34621 ttatcagtcg cctaagaaaa aaaattgaac tagacccatc atctcccatc tatttgcaaa
    34681 caataaaagg aattgggtat caattcaaca cggaggtatg aaaatggatg aacaaacgat
    34741 aattcttttc ttattcttgc tgtcgattgg tctacttttt ctttccatat ttgctttttc
    34801 caagcttggg catattcaca aacgcctaaa agatatagag gagatactgg ctgatgtaca
    34861 aattggaaac gaaaatcgca aaatactaat taagccatgt gatgttatgg ctccattggt
    34921 ttatcagctt aatgagattg tctacgatta tgaaaataag ttactttctc tgaaaaagtc
    34981 ggataaagct agcaaacaac ttatgacaag tctatctcat gatgttcgta caccattaac
    35041 aactctgatt ggttatctgg atgctgtaca cagtggtata gttatcggac aagaacggga
    35101 ggaatacctt gaaatcgcta ggcgtagagc ctacgacctc aaggattaca ttgatgttct
    35161 ttttgactgg tttcggttga actctgatga atttacgctg tcaatagaat ctgtggaaat
    35221 tgcagagttg tcacgcaata ttttgaaaga ctggattcct atattccatg aaaagaaact
    35281 gaattttgaa attgatattc cagaaaatcg actaatggta aatctcgacc cagatggata
    35341 ttcccgtgtg gttaataatc tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat
    35401 caagattacc atgagtgagg attccagaat ggtattactc cgtgttgatg atgatggtgt
    35461 tgggattgcc agagaaaatt taccacatat ttttgagcga ctgtataagt gtgataaagg
    35521 acgttcagaa aaaggaagtg gtcttggtct atctattgtc agtcaaatgg tggagcgaat
    35581 gggtggtcag atttccgtag aaagtgagat tggaaagtat accgtattta cagtgagctt
    35641 accattaata taaaattgtt tttcccccat gtgacatgta tacacagggg gattttttgt
    35701 aagttataaa gttgcaaggt taatacaagg ttgttgcaag gttaatgtaa gattgaggtg
    35761 gtatgatagc tttaataaac aaaggaggat tttattattt atgaataatt taattattga
    35821 aacaaaaaat ctcactaagc aatatggtgc tcaaaagagc gtttctaatt tgaatattca
    35881 cgttcagaaa ggtcgcattt acggattgct aggaagaaac ggtgcgggta agacgacaac
    35941 tatgaaaatg ttattgggtc taacaaaacc aactactgga gaaattaaaa tatttggtca
    36001 ggacattcac aataacgaga agaagcttct tcctcgaatt ggatgtttga ttgaatcacc
    36061 agggttttat cctaatctga caggaacaga aaatctcaaa atctttgcaa ggcttagagg
    36121 aataccaaaa aaaaatgcag tagaatcagc actggatgta gtcggtttgc caaacaatga
    36181 caaaaagtta ttttctcaat actctcttgg tatgaaacag aggttggcga ttgctcttgc
    36241 cattatgcat gacccacaac tgttgatttt ggacgagcct atcaatggac ttgatcctat
    36301 tggtattgca gaagtaagaa agtttattcg tgatctctgc acagaacgag gtaaaactat
    36361 tttgatttcc agtcacattc tttctgaaat tgccttgttg gcagatgaca ttggtattat
    36421 tgaccatggt attttgttgg aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa
    36481 atacatacat tttgctgtat ctgatgcagc acatgcagca aggcttctgg aaacccaact
    36541 taacctgaaa aatttcaaag tagcagataa ttacaatatt atgctatatg atacagatgt
    36601 ttctgttgca gaaataaatc gtgctttcat tctaaacaac attgaggtat cagaatctca
    36661 tttatgcgaa gatactcttg aagattactt caagaaagta acagggggtg aaggaattgc
    36721 ttgatataat ttcatgtgaa ttttcaaaac tcaagcggtt aaaatttata ctgatttcta
    36781 ttttaggtgc ttgcttattt ccaattcccg caacaatttt gattgcaaag gacaacttgc
    36841 cctttgagca attgttcaaa ttagttgtca attttggata ttttttgcta ttaccaattg
    36901 tattaagtat tgttgctagc caactatttt ttatagaaag agataacgat gtattaaaaa
    36961 atcttgcaac agtgccagtg ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc
    37021 tgtttatatc attgttctac tccgttgcag ggttaggagc tactatcatt ggaggtttca
    37081 ttgttggaat agtagagggg gttactgtaa aattaggaat gagcattgct tttggaatta
    37141 tgttttttgt tactgtactt cctgttgtgg tactcattgt atatttcaat cgcagctata
    37201 ttttttctat tatactttct tttgtatatg ctattttcaa ttttagtata tccttaaata
    37261 ttatcaattt tgaaccaaat aacccactat taagcgtatt acctgctcct gttataatgc
    37321 gttggtggat ggcattttgg ggagatccca ctggcgaata cacggcactt agacagccat
    37381 atttgctctc aactcctgct tgtgtaggta ttttatgttt gataaccatt atagcagtcc
    37441 tgctgatttg tatgatatac aaaaagcagg agaattagga ggtatctatg cttgatataa
    37501 ttactacaga atttcaaaaa ataaaaagat ataatattct atggattggt gttgttgctg
    37561 ttctgttttc agcactattg gcagtttttc aggttgtaag ttcacacggc agtgaccctc
    37621 taacgtatga gagctttgca aacggagtta tctggaataa tttcagtctt agttttccat
    37681 ttgggattac acttattgga ggatatctta ttaaccgtga atatacagac caaaccttaa
    37741 aaaacatcct aactgttcct atttcattac gcaaattgct agtgggaaaa ttaattgcgg
    37801 ttggaggaat tgctgtattg ttaagtcttt tcagcttctt atgcacattt attttggggt
    37861 tgttattttg caatatcaat atatctgtta cactcattgt aaaatcctta gttcagattt
    37921 tgtctgttaa tctgtattgt tatgttgctg ttttgccgat tattgcttat tttggtcgta
    37981 aacaaaatgc atttctgacg ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt
    38041 ttgttgctgg aagaaatctg acagactttt atccaatcac agcaggtctt ggtctagttg
    38101 gatacaacaa tggtgctggt actgtttatc agccacttat cggtgctatg actttgattg
    38161 taattctaat cttgacaact attctacttg tatttacacc taattatgat aaagttatga
    38221 ccatatctaa gaaaaaaggg ataaagcata aaaagtattc tcataaataa cataaatatt
    38281 gtttctatta tttaagttgc tagacagtga aaagaaaaaa tctgtcgtac agtggctata
    38341 tgctcatgta ttcaagaagc tacagttttg atttttcata actgtagctt ctttttgtat
    38401 ttattaaatc acggctatca taatacttgc aataggtata gcgacaagag agagtactgt
    38461 catgtgtgtt ttatatatta cattaagtat attctaaaat atatgtttta aataatatat
    38521 ttgaaaatag gaatatttaa ataaaaaaaa taatattata tatagttgac aaaaattggc
    38581 aaatgatata atatataaat atagaaatag taatcattac tattattaaa aagggaggaa
    38641 ttaattatga acttaaaagg aactaaaaca gaaaagaatt taatggcagg atttgcagga
    38701 gagtcagaag caagaaataa atatacatac tacgcttcaa aagcaagaaa agaaggttat
    38761 aatcaaatag ctgctatatt tgaagaaact gctaacaatg aaaaggaaca tgctaaatta
    38821 tggtttaaac ttttacatga tggtatgcct tcaacagagg aaaatttaaa ggatgcagca
    38881 gcaggtgaaa actatgaatg gactgatatg tatgcaaaat ttgctaaaga agctagagaa
    38941 gaaggatttg acaagatagc ttatttattt gaagctgttg gaaaaataga gaaagagcat
    39001 gaagaaagat acttaaagct tttagaaaac ttaaatgaag gaaaaatatt taaaagagat
    39061 gaagaagttg tttggcaatg tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca
    39121 gaaaaatgcc cagtatgtga ccatccaaaa gcgtatttca atataaaagc tgaaaattat
    39181 taaaaattat cattattaaa aaggaatgtt aactatgaaa ttttctaaac aacgagaact
    39241 gattttaaat gaaatattaa ataatccggt tcatcctact gcggattacc tatatgaaaa
    39301 cttaaaaaaa gataatccaa atttaagctt aggaactgtt tatagaaatt tagctcagct
    39361 aacggaacat ggctttataa gaaaggttag tattccaggg tatccagata gatttgatgg
    39421 tcgaatagat aatcactatc atatcatatg tgaagtatgt ggagaggtat acgatttaga
    39481 atccgaagtt cttaataatt tacaagaatt aatatctgag gagacagaca taaaaataac
    39541 atcttataac ataagtttta aagggatttg taataattgt aaaaggtgta gccaagtagg
    39601 ttagttttca aattctaaaa ataattaatg aggtgagttt atgtgtagtg aacaaaaatt
    39661 ttttatatgt gagacttgtg gaaaccttgt aggaatgata caaagtggtg gtgtacctat
    39721 tttctgttgt ggaaaaccta tgaaggaact tgttcctaat acaacagatg cagcagttga
    39781 aaaacatgta cctgtaattg aagtggatgg aaataatgtt actgttaaag taagtagcac
    39841 aactcatcca atgactaaag aacatcatat agcatgggtg tatcttatga ctgaacaagg
    39901 tagtcaacgc aaatgtctag cagtagatgg agaacctgta gttaaatttg ctttgaatga
    39961 tgatgataaa gtgatttctg cctatgctta ctgtaattta catggattat ggaaggctga
    40021 actgtaatat ttaaataaat tgagtcaaag aggaggctat aaatgtattt tcatacaaat
    40081 agcctcctat aaatttatat aaattcaagt tttaattttg tttaatgtca atataatata
    40141 aggagaaaat aatatgtcaa tttataaatg tagtgtttgt ggatatattt atgatgaatc
    40201 taagaatgat aaaacatggg atgaattaag tgaagattgg gaatgccctg tatgtacaaa
    40261 aggtcgtagt tattttggaa agataagcac tgtttactat gaagaagatg aaaagatagc
    40321 agaagacata gttgaaaatg aatctaaatt aaatactgaa aaagagggag atttaaatta
    40381 cttaagtaca tatttaagaa gagatgatga agttgagaaa catatggata taattcacga
    40441 aatggctgtt acaggaaaat ctataataga acctatgaga actaaactac cagtaatttc
    40501 ttgggatgat attttaatta tgggtgctca attaaatcca ttacctttaa atgagcatga
    40561 tgaagtaaat acaacaacaa taataggaaa aaaagcaaag aaacctatga ttatagaaaa
    40621 tccagtttat atttcccaca tgtcatttgg agctttgtct aaagaactaa aaatagcttt
    40681 agcaaagggt gcagcccaaa ataagacagc tatgtgtagt ggtgaaggag gtatattacc
    40741 agaagagaag gaagcttcat acaagtatat ttttgagtat gtaccaaata aatatagtgt
    40801 aactgaagaa aatcttaaaa attctgatgc tatagagata aaaattggtc aaggaacaaa
    40861 acctggtatg ggaggtcatc ttcctggcga aaaagtaact gaagaaatag caaaagtaag
    40921 aaataagcct gtaggtcaag atgtaataag tccatcttgt tttgaggaaa tacaatccaa
    40981 agaggattta aagaaactta tagatgaact tagagaagtt tcagaagggc gtccaatagg
    41041 tgttaaaatt tcagcaggtc atattgaaaa agatatggaa tttatagctt atgctaaacc
    41101 tgattttgta actattgatg gtcgtggtgg agctactgga gcaagtccaa agttgcttaa
    41161 agatgcaact tcaattccaa caatatttgc tctttatcgt gctagaaaat acatagatac
    41221 acatggatta gatatagatt tggttataac aggtgggctt cgtatatcta ctgattttgc
    41281 taaggcaatt gctatggggg ctgatgcagt tgctattgca agttctgctc ttatggcagc
    41341 ggcgtgtcaa caatatcgta tatgtggttc tggtaagtgt cctgtaggag tagctactca
    41401 ggatgaggaa ttgagaaaaa gattacatat agaaaatagt gctaatagag tggcaaactt
    41461 tttaaatgta agtttagaag aacttaagac ttttgctaga atttctggtc ataaagatat
    41521 acatgattta agtgttgatg atttatatac tgttaactct gaaatatcaa attatacaaa
    41581 catacagcat gtatagattg aagtttgaaa ctattagatt ttagatagct ataaaagtaa
    41641 atgataaata tattttataa aagagagtgt cttaaaatga actttttagt tcataagtca
    41701 ctcttttttg tatgaagcca aatatattta tcattttagc aatgaaaaag aatcttatct
    41761 ctttattttg agatagactc cttctattta tgaaaattaa agatattaaa ttgtaaaata
    41821 ttctgtgatg tcgtactatt aaactaaaga ttctgtactt taaagtaaat aaaaatttaa
    41881 ggagtgctta ttatggaaag attgaagata caagttttag ttgacaataa tacttatatt
    41941 gatagatact ttgttggaga accagcagta agctattata ttgagattga tggaaatagg
    42001 attttgtttg attcgggata ttcagatgta ttcatatcaa acgctgaaaa attaaatata
    42061 gatttaggaa atttaactca tgttgttttt tctcatggac ataatgacca tacaagaggt
    42121 atccagtttt tagaaaatag gtatgattta tctacagttg aacttatttc acatcctaac
    42181 tgttttatac ctagaaaaca tggagagaaa agcataggag ctccattttc tgctgaggaa
    42241 attaaaaata tttttatgta taatcctaag gataagccat ttaatctaag taagaattgt
    42301 gtgtttttag gtgaaatacc ttctataaat aattttgaaa aaagagctaa aattggtaaa
    42361 tgtaaaatag gagatttgtg ggaagatgat tatgttttag atgattctgc tatagtttgt
    42421 aaaactgata aaggtatttt tattgttaca ggatgttctc atagtggaat ttgtaatata
    42481 gtagagtatg ctaaaagggt atgtggagat gatagagtta ttggtatact aggtggtttt
    42541 catctttttg aattgaataa taggttagat agtacaatac aatatttgga taaagaagaa
    42601 ataagaatgt tgtacccttg tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa
    42661 atattgaata taaatgaagt tggagtaggg ctgaccataa gtatatagta tttgaaagta
    42721 tataagattt aagttgaagg ttaaaaagat attttctata taaagtatct tttttgttac
    42781 gtttatttta agagaaatca atatactttt atatgaaata ttgcaaaaaa atttattttt
    42841 aatgagaata tattttcatt aataaa
//
LOCUS       DAEPOG010000012        41139 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEPOG010000012 DAEPOG010000000
VERSION     DAEPOG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.012"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..711
                     /locus_tag="KR432_000570"
     CDS             <1..711
                     /locus_tag="KR432_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639125.1"
                     /translation="NFFGNIYYMKSEFDKILEKELNDEIRIEVINSLYKQIQEQRNNL
                     DLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNLLSDLSYNISSYLNEVEKSLDN
                     IIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRLNHLVENMYPYYVDETSREMFK
                     LFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLNNSTKERRKDIIEKIYNSVDYG
                     TNKLDENMFFTGGIKYVK"
     gene            701..1021
                     /locus_tag="KR432_000571"
     CDS             701..1021
                     /locus_tag="KR432_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639126.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            1219..2088
                     /gene="galU"
                     /locus_tag="KR432_000572"
     CDS             1219..2088
                     /gene="galU"
                     /locus_tag="KR432_000572"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0639127.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            2113..3126
                     /gene="galE"
                     /locus_tag="KR432_000573"
     CDS             2113..3126
                     /gene="galE"
                     /locus_tag="KR432_000573"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH0639128.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            3238..5172
                     /locus_tag="KR432_000574"
     CDS             3238..5172
                     /locus_tag="KR432_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBH0639129.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            5327..6190
                     /locus_tag="KR432_000575"
     CDS             5327..6190
                     /locus_tag="KR432_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBH0639130.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(6398..7279)
                     /locus_tag="KR432_000576"
     CDS             complement(6398..7279)
                     /locus_tag="KR432_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0639131.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            7692..8540
                     /locus_tag="KR432_000577"
     CDS             7692..8540
                     /locus_tag="KR432_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH0639132.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            8619..10541
                     /locus_tag="KR432_000578"
     CDS             8619..10541
                     /locus_tag="KR432_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0639133.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            10623..11486
                     /gene="aroE"
                     /locus_tag="KR432_000579"
     CDS             10623..11486
                     /gene="aroE"
                     /locus_tag="KR432_000579"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0639134.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            11686..12903
                     /locus_tag="KR432_000580"
     CDS             11686..12903
                     /locus_tag="KR432_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639135.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            13139..15004
                     /locus_tag="KR432_000581"
     CDS             13139..15004
                     /locus_tag="KR432_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0639136.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(15182..16546)
                     /locus_tag="KR432_000582"
     CDS             complement(15182..16546)
                     /locus_tag="KR432_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0639137.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(16693..18036)
                     /locus_tag="KR432_000583"
     CDS             complement(16693..18036)
                     /locus_tag="KR432_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0639138.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(18109..18210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18540..19193
                     /locus_tag="KR432_000584"
     CDS             18540..19193
                     /locus_tag="KR432_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0639139.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            19334..20605
                     /gene="brnQ"
                     /locus_tag="KR432_000585"
     CDS             19334..20605
                     /gene="brnQ"
                     /locus_tag="KR432_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0639140.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            20807..21994
                     /locus_tag="KR432_000586"
     CDS             20807..21994
                     /locus_tag="KR432_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBH0639141.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(22224..24248)
                     /locus_tag="KR432_000587"
     CDS             complement(22224..24248)
                     /locus_tag="KR432_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639142.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            24638..25306
                     /locus_tag="KR432_000588"
     CDS             24638..25306
                     /locus_tag="KR432_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639143.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            25308..25793
                     /locus_tag="KR432_000589"
     CDS             25308..25793
                     /locus_tag="KR432_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0639144.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            25854..26975
                     /locus_tag="KR432_000590"
     CDS             25854..26975
                     /locus_tag="KR432_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBH0639145.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            27256..28446
                     /locus_tag="KR432_000591"
     CDS             27256..28446
                     /locus_tag="KR432_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0639146.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    28580..28835
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28894..29895
                     /locus_tag="KR432_000592"
     CDS             28894..29895
                     /locus_tag="KR432_000592"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH0639147.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            29999..30388
                     /locus_tag="KR432_000593"
     CDS             29999..30388
                     /locus_tag="KR432_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBH0639148.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            30549..31757
                     /locus_tag="KR432_000594"
     CDS             30549..31757
                     /locus_tag="KR432_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0639149.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(31844..32920)
                     /locus_tag="KR432_000595"
     CDS             complement(31844..32920)
                     /locus_tag="KR432_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0639150.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            33149..33676
                     /gene="hpt"
                     /locus_tag="KR432_000596"
     CDS             33149..33676
                     /gene="hpt"
                     /locus_tag="KR432_000596"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0639151.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            33765..34697
                     /locus_tag="KR432_000597"
     CDS             33765..34697
                     /locus_tag="KR432_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBH0639152.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            34759..35985
                     /locus_tag="KR432_000598"
     CDS             34759..35985
                     /locus_tag="KR432_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0639153.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            36069..36290
                     /locus_tag="KR432_000599"
     CDS             36069..36290
                     /locus_tag="KR432_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0639154.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            36479..36649
                     /locus_tag="KR432_000600"
     CDS             36479..36649
                     /locus_tag="KR432_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639155.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(36742..37203)
                     /locus_tag="KR432_000601"
     CDS             complement(36742..37203)
                     /locus_tag="KR432_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639156.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            37386..37853
                     /locus_tag="KR432_000602"
     CDS             37386..37853
                     /locus_tag="KR432_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639157.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            37896..39659
                     /locus_tag="KR432_000603"
     CDS             37896..39659
                     /locus_tag="KR432_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBH0639158.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            39667..40275
                     /locus_tag="KR432_000604"
     CDS             39667..40275
                     /locus_tag="KR432_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0639159.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            40347..40916
                     /locus_tag="KR432_000605"
     CDS             40347..40916
                     /locus_tag="KR432_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0639160.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT        15736 a         4564 c         7096 g        13743 t
ORIGIN      
        1 aatttttttg gaaatattta ctatatgaaa tctgaatttg ataagatttt agaaaaagag
       61 ttaaatgatg aaataagaat agaagttata aattcacttt ataagcaaat acaagaacaa
      121 agaaacaatt tagatttatt gtcagcatat atgccaagaa tattagaaaa tcaagaaata
      181 gatttgtttg aaaagtataa tactaaactt gatgtacaag tgtttaagca gattaaaaat
      241 ctattgtctg acttatcata taatataagc agttatttaa atgaagttga aaaaagttta
      301 gacaatatca tctttgtaat ttcaggagaa gataagaaga aatacaatta tagaatgtta
      361 aaagaaagtg aaagtgcaag tcttgatgta tttttaaaac aagccatatc aagattaaac
      421 catttagtag aaaatatgta tccttattat gtagatgaaa ctagtaggga aatgtttaag
      481 ttatttgagt tattgtggaa aaactataga aatattgatg atgatagtat aaaagaaata
      541 ttattcgaga gaataacaaa tacatatgta tttaaacaat tgacattaaa taatagtaca
      601 aaagaaagaa gaaaagacat aattgaaaaa atatataact ctgttgatta tggaaccaat
      661 aaactagatg aaaatatgtt tttcacagga ggaataaagt atgtcaagta aatacaatgt
      721 ttggactttt aactatgagt tcttaggatt ggactcaggg gatgaaaaaa ataatgaagt
      781 ttattacgag attgatttag atgaaatttt aaatgaaaac tatatagatg aagatataga
      841 tagtggttta gattctgatg gaaatagtat tttaggagaa ttaatatcag aaatggatat
      901 agatgaggat gtcagtgttg atatgacata tcaagaacta gaagttgact tagaagattt
      961 agattcttat ctagatgaag atatagatag tgatataaaa ggcatcttag atgaaattta
     1021 gtacctgtat aagttttgga taaaatataa acaatacaat attaacttat aaaactaaag
     1081 gtagatgaaa tttcatctac cttatattat cttatagcat taaatattgg gataatatag
     1141 agtttgaaga aaaagtataa atatggtata ttaatttggt acaatagaaa atttacgaag
     1201 aagggtgaaa aaatgcagat gacagttaaa aaagcaataa taccagcagc agggctagga
     1261 accagatttt taccagcaac aaaatcacag cccaaagaaa tgcttcctat agtagataaa
     1321 ccaactttac agtatataat agaagaagct atagaatcgg gaattgaaga aattcttata
     1381 ataacaggta gaaataaaaa atctatagaa gatcattttg ataaatcagt tgaattagag
     1441 ttagaactag agcaaaaagg taagacagag atgttagaaa tggtaagaga catatcaaat
     1501 atggtaaata ttcattatat aagacaaaaa gagccaaaag gtcttggaca tgcaatttac
     1561 tgtgcaaaaa gttttatagg tgatgaacct tttgccgtat tattaggtga tgatatagta
     1621 gattcagatg ttccgtgctt aaaacaatta atagatactt ataatgagta taaaacaact
     1681 gtattaggtg ttcaaaagat agctaaagaa gatactaata aatatggtat attggatgta
     1741 aaacatatcg aagatagagt atataaagtt aaagatatgg ttgaaaaacc tgcaattgaa
     1801 gaagctccat ctgatatagc tatacttgga agatatataa taactcctgc aatatttagt
     1861 atattagaga aacagacacc tggtaaaggt ggagagatac aactgacaga tgcacttcaa
     1921 actcttggaa aacaagaggc tatttatgct tataactttg aaggtagaag atatgatgtg
     1981 ggagataaga ttggtttttt agaggcaaca atagactttg ctctaaagag agaaaattta
     2041 aaagatgatt taatggacta tatgagaaaa aaagtaagtg aaaaataaaa tattagaaaa
     2101 gaggtaatta caatggcagt tttagtagct ggaggagcag gatatatagg aagccataca
     2161 gcaatagaac ttttagaatc aggttatgaa gttgttatag ttgataactt aagtaatagc
     2221 aactcaatag tggttgatag aattaaggaa ttatctaaaa aaccagtaaa attttataat
     2281 attgatatca gaaataaaga tgaaatgcat gttgtattta aagaaaataa tattgaatca
     2341 attatacact ttgcagcttt aaaagcagtt ggtgaatcag tagaaaaacc aattgaatat
     2401 tacagcaata atctaataag taccttaaat ttatttgaat taatgagaga atatggtgtt
     2461 aaaaagtttg tatttagttc ttcagctact gtatatggag acccacatac ttgtcctata
     2521 ttagaggatt tcccactttc tgtaaccaat ccatatggaa gaactaaact tatgatagag
     2581 cagatgttag tagatatatc aaaagcagat aagtcattgg atatagctct tttaagatac
     2641 tttaatccag ttggtgctca taaaagtggt agaataggtg aagaaccaaa tggagttccg
     2701 agcaatctaa tgccttatat aacaaaaata gcagtaggta aattgaagga attgagtgtt
     2761 tatggtaatg attatccaac acatgatgga actggagtta gagactatat acatgtatta
     2821 gatttagctg ctggtcatgt aaaagcatta caaaaattag aagaaaatcc aggacttgtt
     2881 gtttataatt taggaactgg aaagggttat agtgttcttg atttagtaaa agcattttct
     2941 aaagcaagtg gaaaagaaat accatataag attgtaggaa gaagggctgg agatgtagcc
     3001 atgtgttatg cagattcttc aaaggcagaa aaagagcttg gatggaaagc taagtatgaa
     3061 cttgaagaga tgtgtgaaga ttcatggaga tggcaaagta tgaatcctaa tgggtatgaa
     3121 gagtagaata catacttata tgtataaatg taacaaattt gtaaatataa ggtattggta
     3181 attttatgtt ataattagac acatgtagta atgtttctaa aagaggggga agttatagtg
     3241 aaaaaaagtc taaaaaagta tttagtacta gcactgactt tagttttagt atttgcatgt
     3301 ggcgtaccag aatcaagcgc agccagtaaa catgtgatta tagtaaattc aagaaagaat
     3361 actctaggat attttgtaaa taataaactt gtaaaagaat ttagagttgc aacaggtaaa
     3421 aaggggtcag aaacaccaac agggaaaaca aaagtagtta acaagataaa aaatagacca
     3481 tattataaag gtaatatacc tggaggaagt ccaagaaatc cattaggaga tagatggatg
     3541 ggattagcat taaaaggaac ttatggagat acatatggaa tacatggaaa taataatgag
     3601 agttctatag gaaagcatat ttctggtgga tgtataagaa tgcacaataa agatgttaga
     3661 tggttatttg accaagttcc tgtaggttca gatgtaatta tagattatag taatgacagc
     3721 tatgtaaaga tagcggcaaa atataagata aatctaaatc aaacaggatg gaaaacagaa
     3781 aatggtaaga agtattatgt aaaatctgat ggaacatacc aaaagaatag ttggctaaaa
     3841 gtaaatggaa aaatgtatta ctttgatgct tcaggagtaa tgcaaacagg atggaaagca
     3901 attaataaca aaaaatatta tttaggaact gatggagcaa gagtatctgg atggaaagta
     3961 atagacggaa aaacttatta ctttaactca gatggagtaa tgcaaacagg atggcaagaa
     4021 aagaatggaa agaaatacta tctaggatct gatggtttat cagtaacagg atggcaagaa
     4081 atagatggta ataagtatta ctttgacaaa acaggaataa tgcagacagg atggcaacaa
     4141 atagatggta agtcttacta tttagacaaa gatggaaaga tgctaactgg ttctcaaaaa
     4201 atagatggga aagattatac ttttaatgaa gatggaacta taaatcctac atgggataca
     4261 attataggtg caaatagatt tgatacagct aaaaagataa gttcagttgg aaactggaat
     4321 gctgatagtt cagatactgt aatattagta aatggtaatg caatagctga tggtataaca
     4381 gcaactcctt tagcaagtag ctatgactca acaatactac ttactaatac agctaattta
     4441 ccaactgaaa ctgttgaaga aatgaagtta ttagcaccta aaacagtaat cttgataggt
     4501 ggagaaaatg caataagttc taaattagag caagaaataa aaactacttt caacacagag
     4561 acaaagagaa tagcaggtca agatagatat caaactgcta caagaatagc tgaagaactt
     4621 ggaaatcgag aagaaataaa aactgcatat atggtttctg gaaatgggga agcagatgct
     4681 ttatctgtag cttctaaagc tggggaagaa aaacaaccta taatattagt caataaagat
     4741 ggaattacag aagaatctta taaatggcta acagaaagaa aattagaaaa tgcatacttt
     4801 ataggtggac caagtgcaat taacgattca gtaatagcaa aaatgaatga tataactact
     4861 gaagatattt caggaaatag aatatatgga gatagtagag tagatactaa tgccaaagta
     4921 atagaaaaat tctatggaga tactgattta caggctgtat tagttagtaa atcagatgct
     4981 ttagtagatg ctttatcagc tggaccatta gctgtaaaat tacattcacc aatagtttta
     5041 atggataatt caggattatc attagagcaa caaagagtat ttgcaaataa aaaagttgaa
     5101 actccttatc aaataggagg cggagtatct tatatagtaa tggataaatt aatggatatt
     5161 cttgcaaaat agtaaaaata tatattgaaa aataattata attgagatgt gaaattcaat
     5221 caataaaagc ttgattgttt tacatctcaa ttttttagta taaaattaag tttaagttat
     5281 ataaaaatct tggaatattt ttaaaactca tataaaatga atatagatgc tatcatcata
     5341 tgttaagagg gtgagtaaga ttataaagaa aaaatatata atgtttattg atgaaagtgg
     5401 aagagctgat ataaatcatg aagacccatt tacattgaca ggagtaattt ttgaatataa
     5461 atatgcagta agtcaaggcg attatgtatg ttctttgagg aaagaaatgg atgcatttaa
     5521 agagtattgt tttggaacca gtaacatagg aatacatttg acagatatat caagaaaatc
     5581 aagagcattt gagaagtttg atgattcaca gattaagtta ttctatgatg agttgccttt
     5641 atttctatct agattagagt gtactataat atcagtaaca gtagataaag ctagattaaa
     5701 gaaatattat gcaccttcaa aggaaccgta tgttgtggct tttatacatg ttcttcagaa
     5761 tttttattca tttgtaaata atgaaactgt agaatctgca cgaatagtta tagagtcaag
     5821 ggatgatgtc tcaaacttaa aagtacaaaa ggcgtttttt gatgtattta ataatggaac
     5881 aatatactta gatatagatg aagaattaag agataaggtt aagggtttta taattgccaa
     5941 aaaagaagat gctgactata gagaaggttt agaaatagca gatattttat gtaatcctct
     6001 tagcagagca agaagagggt taatagaagc taatccaaag tgtatgcaat atggagaaaa
     6061 aaacaaaata ttcaaatctg tcagaaacaa aatatatact ccaacgagtg tgaatgatat
     6121 aagaaattgg ggatttaaag aagttcctat tgtagagcct gttggaccat ggcttgaaaa
     6181 aatgcgttag atacaattga caaatttaaa aaaatctgat ataatgtaaa taacctagtt
     6241 attcaggaac tgaatccagt tgctgttgta taacgataat tactgagact cggatgccga
     6301 ctgtcagtta ggaatctata cttttaaaag tatagatttt tttattttat aaaaagaata
     6361 aaagaggata tttaaaaata acccctttat taataaatta taattgagcg tttttaatga
     6421 caaagttttt aaagttttta attgcaggag gcaaatatct atcttttata aatgctaagt
     6481 aaaagtttct ttcccaactt gggtgaataa tctgaattac ttttactttt gtaaagctaa
     6541 gcatattcat atttgggact acagcaattc cgaaattttt tgcaactaat ccagcaatta
     6601 cttggtcttc ctctacttca tagactatat taggctgttg atttattttt ttaaacatat
     6661 catcaattat atatctaaga ccacttctct gattaaatac tatttgagga taaggtatgg
     6721 tttcttttaa atctatagta tcctttgcag ctagtggatg actatatggt acaattagta
     6781 ctaaatcttg tttggcaaca ggtataaatt caattccctt ttctttttca agtttcgaac
     6841 aaaatgcaat atcatatttt ctttctttaa gaccttgaat aatgtcagta gttacaccag
     6901 tatgaaattt aaaatctatt gatttagcag gattagattt taaaaattta tgcactatgt
     6961 ctgggataaa atcagtacca agtgttctaa gaaaagctaa gtctatttgt ccttcaccag
     7021 taccagttat ttttaatgat tttacacttg aatctaaaat ttctaatgat ttttccacat
     7081 cttttaaaaa tatttttcca tacttagtaa ggactatatt tctaccttct ttttcaaata
     7141 gagccacccc aagttcattt tctagtaaag aaatagcatg acttaagctt ggttgtgtta
     7201 tagataaatt ttctgccgct tttgtatagt gttctaaatg tgccaatgta acaaaatagc
     7261 gtaagtgata taaattcaat ttttgttcat cctttcatag gtatttttat ttatatattt
     7321 gtagaagtta acctttaaga tatttttaca tttttattat atatgattat agagtaagaa
     7381 taaaagcttt taaaaaaatt tatcgttttt tgtctgaatt ataagataaa tatatttata
     7441 caacctaata gaaaaataca agaattatct tataaataaa atctataaac aaaatctatg
     7501 aattaatagg aaattatcaa tttgttatta gtaaagtctc gtgatacaat aaatttataa
     7561 aatctagata aatagaaatc aaaaaatact tttattttta aggtaaaata atactactag
     7621 taggataaat aaaaatataa aatcaaagat actattaatt ataaagaaaa taaagggggc
     7681 aaatttaagc tatgaataat caaaaaaatc ttaatattcc aattacaatt agttcttata
     7741 cattgggaac agaagtttca ttccgtgata gagttcgtat tgcaaaagaa gctggatttg
     7801 atggtattgg attgcgtgct gaaaattata ttgatgcaaa aaatgcaggt gttacagatg
     7861 aagaaatgat tgcaattctt aatgaatatg atatgaaagt tacagaagta gaatatataa
     7921 ctcaatgggg aacagaagaa gatagaactg aagctcaaag agaaaaagag caaaatgttt
     7981 atcatatggc tcacttattt aatgttaaac atataaattg tggattatta gagatgatac
     8041 cagaagaaca aattataact gcattgggag aattatgtga cagagcaggt gaacttatta
     8101 taggtcttga gtttatgcca tatagtggtg taccagattt ggctactgca tggcgtgttg
     8161 tgaaaggatg taatcgtgaa aatgcaatgc ttatattaga tacttggcac tgggctagag
     8221 caaagcagac agcagaggac cttgcacctg ttccagcaga taagatagta tcaattcaag
     8281 tgtgtgatgt tttagaaact ccttatgaaa aacttaggga tgaatcacta catgaccgtt
     8341 tggctccagg tgaaggatat ggaaacacag aggaatttgt gaaaataata aaagaacaca
     8401 atatatcacc aagagtcata ggggcagaag taatatctga ttctctaata tctaaagggt
     8461 tatccactgc tgctgaaact gtatttaatg caactaaaaa agttttggat aaagcatggt
     8521 ctgaagtatc tccaaaataa acttatataa tataaaaata tacagagtat tataatataa
     8581 aacttttaaa tataaaactt gaaaggaagg aaaaccttat gaagattagt tctatgttta
     8641 cacctataag aattggttca atgacagttc caaatcgttt tgtagttccc cctatgggaa
     8701 acaattttgc aaacacagac ggtactttaa gtgaaacttc taaggcttat tatcttgagc
     8761 gtgctttagg tggttttgga ttaataacta tagaatctag tgttgtagac aaaaaagcaa
     8821 aaggaggtcc tcgtaagcct tgtttatatg atgatagcac aatagatagt tttaaaaatg
     8881 taatagatgc ttgtcatgat gcaggttcga aggtatctat acaattacaa catgctggtt
     8941 ctgaaggaaa tgaaaaagta gcaggacatc cattaaaagc tgcttctgca atacctgcat
     9001 caaatggtcg taatacacca ctagcaataa caacagaaga aatatatgaa cttatagaat
     9061 cttatggaga tgctgcatta cgtgcacaaa aagctggtgc agatgctgtt gaagttcact
     9121 gcgctcatgg atatctggtt agtagtttta tatcacaaag aacaaataag cgtgtagatg
     9181 agtttggtgg atgttttgaa aatagaatga gattacctcg tcttatcatt gaaaatatac
     9241 gtaaaaaagt aggaaattct ctagcaatat tatgtcgtat aaacagcact gatgatgttc
     9301 caggaggaat tgatatacat gatagttcag taatagcagc atatctagaa ggttgtggaa
     9361 tagatggact tcatgtttca cgtagtatac atatacatga tgaatatatg tgggctccta
     9421 cgactttaca tgctggattc agtgcagaat tagttactga aataaaaaaa gctgttagta
     9481 taccagtaat aacagttgga cgttatacag agccacagtt tgctgagtta atggtcagac
     9541 aaggaagatg tgatttagta gcattcggta gacaaagctt ggctgaccca gaaatgccta
     9601 ataaagctaa aaatggaaaa ctagacgaaa tgataccatg tattgcatgc ttacaaggat
     9661 gtgtaccaaa tatgttccaa ggaaaaccta tagcctgtct tgcaaatcct atattaggac
     9721 atgaagcaga gcttaagcca gctgaaatta gcaaagaagt tcttgttgtt ggtggaggtg
     9781 ttggaggtat gcttgcagca tgggtatgtg ctaaaagagg acataatgta actttagttg
     9841 aaaaatctga agttcttggt gggcaaatgc gtttagctgc atatcctcca gggaaaggag
     9901 atattactaa cttagttaga agttatattt caaaatgtaa tcaatatggt gtaaaaatat
     9961 gtactaatac agaagctaca gtagaactta taaaggagaa atcacctgat gtagttatta
    10021 tagctactgg agcaactcca cttgtacttc ctataccagg aatcaatgat tcaggattaa
    10081 ttcatgctgt agaccttctt gatggtaaaa aatcttgtgg gaaaaaagta cttgttgttg
    10141 gaggtggaat ggttggatgt gaagtggcag ccttccttgg agaacaagaa catgaggtta
    10201 ctgttattga gcttagagaa gaagttggtg cagatgtaat ttcagaacac cgtaaattcc
    10261 ttatgaatga ttttgaaaat tataatatac ataccattac tggtgcaaaa gtttctaaat
    10321 tttatgatga tggtgtcgac tatttactta cagatggcac agagcatgat ttaaaaggtt
    10381 ttgattcagt agtacttgct atgggttctc gtaattacga cccattaagt gaagttataa
    10441 aagaagttgt aaaagaaact tatattgttg gtgatgcagt aaaagctcgt agggcattag
    10501 atgcaacaaa agaagctttt gaagtagcat taaatatata aataataaat aaatttttct
    10561 ttgtaaataa ctgaataggt tatttgcaaa gaaaatgata atattgggag gaaattatag
    10621 ttatggaaat ttcaggtaga acaggattat ttgcattaat aggtacacca gttggtcact
    10681 ctaaatcacc agtgatgtat aattatagtt ttaagaaatt agatttagat tatagatatt
    10741 tagcctttga tatcacagta gataaggtaa aagaggcact tttagcaata aaaactttta
    10801 atataaaagg agccaatgtt actatgccat gtaaatctgc tgttacagaa tatatggatg
    10861 aactttctcc agcagctcgt ataattggag catgtaatac aattgtaaat gacaatggaa
    10921 agttagttgg tcatataaca gatggagtag gctatgttcg taatttaaaa gaaaatggga
    10981 tagaagttaa aggtaaaaag attacaatta tgggtgctgg tggtgcagca actgctatac
    11041 aagtacaatg tgctttagat ggagcaagag aaatatctat ctttaatgct aaggatgatt
    11101 tttataaaag agcagagcag acagttgaga atataaaaaa agatgtacca gaatgtgttg
    11161 ttaatctata tgatttagaa gatacaaaca aactttatga ggagatagaa tcaagtgata
    11221 tattgacaaa tgcaactctt attggtatga agccttatga caatgaaaca aatataaaag
    11281 atactagtgt tttaagaaaa gacttagtag taacagatgt cgtttataat cctaagaaaa
    11341 caaaaatgat tgaagatgct gaagctaatg gatgtaaggc tattggtgga ttaggaatgt
    11401 tattatacca aggagcagaa gcttttaatc tatatactgg attagaaatg cctgtagaag
    11461 aagttaatga attatgcttt aaatagataa gagtgatatc ttaaacaatg aaaaaacata
    11521 cgtatttcaa tatttttaat aataaattac aaattgtcat agtgggtttt gcatcatagt
    11581 ccagttaaat ttgaagcttt aactgactat actttaaatc cattatgagg atgagtttat
    11641 tgtacacaaa aatgttaata aattaacaag aatgggagaa aaattatgaa caataaaaaa
    11701 tattatccta ctgccatagc gttgtatttt tcatattttt tacttggtat tggaatttca
    11761 attttaggac aatacaaacc agaattttca tcaatgtggg gggctaaaac actatcagat
    11821 ggaacattgg atgtaagtat tgttttagct gttattgctg cactaggatt agggagactt
    11881 atatcttatc catttgcagg acctatttca gataagtatg gaagaaaagt aagtggtctt
    11941 ataggaaact ttctacatgc aattttcttt gttggaattg tattttcacc aaatttttat
    12001 atagcatatg tatttgcaat aattggaggg gcagcaaatt cattcttaga tacctgtgta
    12061 actccttctt gtatggagat atttgctagt ttaggaacaa tagcaaatat gtttactaaa
    12121 tttacaatag ctttagcaca atttttgctt ccttttataa ttggatttgt tgcagcaaat
    12181 tcaatatcat ttaaagttat ttttatagta acagcgattc ttattgtagt agatgccatt
    12241 ttgatagctg tgttaccatt tccaccagct aataatgtta ttgataacaa aggcaaaaca
    12301 gttaaatcag agaagatgaa atttacacca acaagtatag ctcttgtttg tattggattt
    12361 acatgtacat ctacatttgt attatggtta aattgtaatc aggaattggg taaattgtat
    12421 gggatggctg accctacaaa aattcaatcc ttttactcaa tgggagtaat atgtgcagta
    12481 ttaataactt ctttacttat taaaaaatat attaaaccaa taagaatatt agttatatat
    12541 cctataatag ctttacttat gctattaatt gtatattttg ttcaaactcc aactatttgt
    12601 atgttagggg gatttgtaat aggttattca gcagcaggag gtgttcttca gttaacaacg
    12661 tcaactgcaa atgaaatgtt tccaactaat aaaggaaaaa taacatctat agttatgatt
    12721 gcatctagta tagcaaacta tgttatttta aatatagcag gtattattac aaaatcaggg
    12781 ggagttaatg gaccaaaata tgttgtattg tttaatgtgg caataacatt tgttggaatt
    12841 ttacttgcat tgtttgtaaa catgagatat gaaaaagaaa aagtgtatga ttatgatgtc
    12901 taatattagt ataatatatg catagagaag tctaaatcta ggatagattt ttattaaaag
    12961 taattaaaat taataaggat attatttaca gttataagat agggtattta cataattaat
    13021 aataaggata ttactagaat ttcaatttct agtaatattt ttttgcgtat aaatgtaaac
    13081 ttgacgaaat aagtagtata atcaatataa attacatcat gtaaaggagg ataatttggt
    13141 ggagaatgtt tttaagcgat tgcaggaatt taatggttat gatggataca aagaatcatt
    13201 tgaaatgaat tacctatgta tatatgaaag tataccgtta agagaacaag tagaacttgc
    13261 aaataatcta gtagatgaaa ttttaaatat gtataaaagt gaatctaatg aaatttattt
    13321 attggaggat agtaattcta agtctttaat ttgttatttc gaaattttta tgaaaaagat
    13381 aaatactttg gtaaaagaaa tgattattga tgagaaatgg ttatataaat taactaaaga
    13441 attaatatat aagagtaaaa aagttgagta tgtaaaacta gggttagttt taagtgagaa
    13501 atatctaaat gtagaaaact taagagaagt tgtagataca ttttctaaat ctggagaata
    13561 cgtattttat ctgagtaata ctattaaaaa acttgaattt tacaatacat atttatttaa
    13621 tttatctaaa aaggctacag gttcaataaa agtatttgct attgttaata tggaaaattt
    13681 agattctaaa ataaattcct atctaattga agatggttat aaggatacaa agtacgaacg
    13741 attattgatg aactatatta tttcaattgt agatttgaat gaatatcttg aaaaacgaga
    13801 tttagataaa gaaaagataa ataatttagc acgtcttata tgcaattact tgctaagtgt
    13861 agaatttaaa tatataggta ataaattaga attagtgaat cgatttttac ctactgtagt
    13921 aaattatggg actaactttg aaagtttata ttcaattttc ctaatagcta taaacgtctt
    13981 aaaagatgaa aatatagagt acaataagat tgaatttgaa aaagaaataa atgatatctt
    14041 gctcagtgaa aaatggaaaa atatatattt tgaagcatta agagatgcta gtggaaaaac
    14101 agaagatatt ataaaaatgt cagaaattta tgatgtaaac ttatcttttg atgacttgtt
    14161 gccatatctg aatagagata ttagagattt tgaggtatat tggcatatat ctaaaaaggg
    14221 aacgacaagt tctagattaa aattattaaa tttttttgaa gagacgttta aaatagatga
    14281 tttaattgga aaaatgaaag atatagaaaa agataaattg actcaagaat attatgatga
    14341 tatgttattt tttattgtgc ttaaaggaag taaatcacta tatcctgaag gaaagaatat
    14401 atctttaaaa ggaatatttg gaaatataaa tgaagtaaga aaagaatcca taaatatctt
    14461 aaaaagatac agagagaaac tatctcttga agaattaaaa attgttaaag aagcgtatga
    14521 aaaagaaaaa aatgttatat tgaaggatga attaagaaga gtattatatg aaagtaataa
    14581 tttaaaaaaa gaatttgtaa atattgaaaa aataaaagta gatgaacatg gtaaggatat
    14641 ctatttgact tcaatagctg ttgctggttc aaggtttaga aatagagaat atcttgaaaa
    14701 agaattggag aagagtaaga tatactattt aactagagag aaagataatc tatatgatga
    14761 aaaagctatt aaaatagttg gtgaaactgg atatgtaata ggctatgtcc caagaaaaga
    14821 aaactatatt ttgagcaatt tattagatgg aggaaaactg ctgtattgta gagtaactga
    14881 atataattta tatgaagatt gtatctacgc aaatgtgtac ttgagttata aagatgtaat
    14941 tgaaacagtt gaaaactctt taaagatggt tttagataag agtagaataa aacttataaa
    15001 ctaagacgtt tatatattgc ttagatgatg taatcctaac tgattggtta ttttttctgg
    15061 aattatggtg tattttatga gcaatgttaa tgaatataaa ataaaaaaac aattgttttg
    15121 tatatatgat gtgtataaat atatttatat attcatattt acaaaacaat tgaaatttat
    15181 gttattttat gattttaaat ttagatggaa ttttaacttc tgctttatct tttattttag
    15241 aagcttgttt tttaacttta tttaataatt cagattcatc tacaactaat acttttcttt
    15301 ccttcatcaa ccatttacca tcacaaatag tagaatctac attttcacta gtcatagaat
    15361 aaactatatt tccaattgga ttatgcattg gtaaacaatg aattgtatct ggatttagaa
    15421 taatcatatc agcttttttt cctacttcaa gactaccaat ttctttttct agtaaagcac
    15481 attttgctcc atttatagta gccatttcta agacttcttc agctggtacg acagttgggt
    15541 ttaaagttct acctttatgt attagtgatg taagatacat atccctcata aggtccattc
    15601 tattatttga aggagctcca tcagtaccaa ttgaaacagg tattcctttt tctaacattt
    15661 caggaattga ggcaaatcct agaactactt tcatagctgc acctggatta tgagaaactt
    15721 ttacatcatg taatctaaat aaatcaattt ctttatgagt aagccaaact gtgtgagcag
    15781 ctaaaagatt tggtcctaat acacctagtt tatcaagata ttcaactgtt ccaactccgt
    15841 tgttttgctt tacaaatgaa atttcatcag ctatttcagc aatatgcata tgtatacctg
    15901 tattgtattt atcagctaac tctttagtcc taataagcaa ttcttcagtt gtattaaaaa
    15961 tagttcttaa tgcaaaccat attttaatcc tctcattaga tgtgttatta aacttatgga
    16021 ataagtctaa ttgagtgttg atttcctcat cagtagtttt ttgccatgct tcaggaagac
    16081 catctcctgt atccataact gatttagcta aacaggctct aagtccagtt ttatcaacag
    16141 cttcaaccat agcatctaca tattgaccac cagattctaa aaatgtagtt accccagatt
    16201 ttatcaattc aacacaacat gcagtagatg aaatcaatga atcttcatag ttgaagctac
    16261 tttcgtatgg ccagattctc tctctaagcc aagttaaaag tacaacatca tctgcaagac
    16321 cacgtcctaa ttgttgggat aagtgaacgt gtgtattgac taaccctggc attagtattt
    16381 ttcctttagc atcatatatc tcagcatctt tatctacaat attaatatca aacttaccaa
    16441 cattttttat aatattattt tcaataagta tacttccagt ttcatatacc tctctatctg
    16501 cattcatagt aattagatat gcatttttta taagtaattg tgacataagt acaacctctt
    16561 ttctatatat ttttatatct atgtattatg ataataaatt ttctatacaa agttaatagc
    16621 aaaatcaatt tatacataaa ttgattttgc tatttataaa tttaaaatat tgacctagaa
    16681 ctatttaaaa tattaactta gaactaagtt tacaactgca agtaaaccta taatccaaag
    16741 tgtaatactg attttatcag tttctttagc taccacatgc attattatat atgaaataaa
    16801 tccaaagcat aaaccaatac ttatactata agttaaaggc atcaataaaa ctgtaacaaa
    16861 agcaggtaca gctattttgc tatcagcaaa atcaatctgt tttatgcttt caaacatgaa
    16921 tacaccaacc attacaagtg caggagctgc agcgtaagta ggaactatac caagcaatgg
    16981 tgtaaatata agtgtagcta agaataatgc tgctgttact attgaagcaa gtcctgttct
    17041 tgcacctgct gcgataccag aagcagattc tccaaatgtt gtaacagtac tagttcctaa
    17101 gaatgagcct attaaagtag aagaaacatc tgcatataac attcttccta aatcttttat
    17161 ttcaccattc tcatcttcaa gacccatttg tttacaacaa gtaataagaa atgctaatga
    17221 atcaaacatg tctataaaca tgaaagagaa tataggtcca attaatgaga atttcaatgc
    17281 acctacaata tctaatttaa aagcaactgg tgctatacta ggaggtgttg agattattgc
    17341 tgttggcaac tccacaaatc caaatataac agatattatt gttgcaacta ctatacttat
    17401 caatattcca cctttaactt ttttatattc tagtattgcc attaagataa gagcaaaaag
    17461 agctattaaa actgttggtg taaaatctgc aagtgaaact atagtatctg gattagctat
    17521 tataacaccc atatttttta aaccaacaaa agcaaggaaa agacctatac ctgctgaaga
    17581 tgctactgcc aattctctag gaattgcgtc tgcaattgtc tttcgaagac ctgttactgc
    17641 gagtgtaaag aagacacatc cagatagaaa aactacccca agacctgttt gccatgggat
    17701 tcctgcacct ataaccaaag agtatgtgaa aaatgcattt aatcccatac caggagctag
    17761 agcaaatggt gcatttccta aaatcgcaaa tataagtgta ccaatggctg tagctagtat
    17821 ggtaactgtt ataagggcgg ctttatccat tccagcttca gataatattg ctggatttac
    17881 gaaaactatg tatgccattg ttaagaatgt agtaactcca cctattactt ctgttttaag
    17941 gctagagcca cgttcgctaa atttaaacat tttgtctaaa aagcttaatg atgaattatt
    18001 tccatctggt tgtacattgt tagtgtttgg taccattttt acctccaaaa aactttaaga
    18061 tataaacaaa aaaatatata ttcatataga tagaatagaa catatattca aagttgatat
    18121 tctattcata gaaaacccat ttaaggtgag tttgtagaaa ctattagacc gtattactaa
    18181 tattatatga acattttcta tttgtttttc tgttgtatta tatcgtaata ttgtattatt
    18241 tacaataaat ataataaaat tattcgtttt tttgtcgatt ttataaaaaa ttaaggttat
    18301 ttattggcaa gaattactgt tttcaataat tgaatatgta taagtataat gtcaattatt
    18361 ctaacttgtt ttatactatt atatagaagt ctattataaa tgagtttgtg atataattat
    18421 aaaagtactg aatacacgga tatttcgtaa aaacttttta atatatcaaa tatgtatatt
    18481 aagttaatat attaaaaaaa ataatatcaa aaataatata atttatagtg gaggggaaaa
    18541 tgactaaaat tattaaagtg caaaaaccta aatttacagg agaattagaa attattgaaa
    18601 atcttgaaga tatacttgaa gatatattta atgatgaaaa aatatttaat aaaattatag
    18661 aaaatgaagt tataagtgat ttagattctg taagagttag ctttgaatct tgtatattta
    18721 gaaatgtatc ctttgaaaat tgcaatttaa aaaagattga tttacttgat gtggtatttg
    18781 aaaactgcaa tttatcaaat tcattttttg atggaggaag tatatacaga gttgaattta
    18841 aaaatagtaa attgatagga gttagatttg atgattgtat acttaaaaac gttttgttca
    18901 aggatgtttt aggtaagtat tctaactttt cattctctaa atttaagata gtttctatct
    18961 tagattcaaa ttttgaaaat tctgtatttc aagaagtaaa aggtgacaat ctagtatttg
    19021 agtatacaaa cttaaaaaaa gcaaacttta atggaactaa actttctggc atagatttta
    19081 ccactaataa tattgaggga attgagattg gtatagatga gattagagga gcaatttttg
    19141 atgtgtcaca agcaattgat ttaacgaaat tgatgggaat tatcataaaa tgaaaaaaat
    19201 attcataaaa taaattggga aaacgttaac aatgtgtgaa tttttaccag attgacattt
    19261 ttctgaaaat aaagtaagat tatcttaata ttcaaaatat tttcaaaggg ggtaatttag
    19321 tgtgagtaag cagatgagag atatttttat agttggtttt gctttatttt ctatgttctt
    19381 tggtgcagga aatcttatgt ttcctccatt tttagggatg gaatccggca aagactggtt
    19441 aattcctctt attggatttg ttgttgcaga tgctggtata gcaatgcttg ttattatagc
    19501 aacagcaaga tgcaagggaa gcatggatga tgtacttagg agagctggta aagggttagc
    19561 aagagtaatg agtgtagctg ctctagcatg tttagcactt cttgtaatac caagaacttg
    19621 tgcaacaaca tatgaaatgg gtattatgcc tatatttgga gaccatggac cagtagctaa
    19681 agctatattt tcaataatct tctttgggtt aactttagca tttactatta ggtcttctaa
    19741 ggttatagac ataataggta agtatttaac tccagcgtta ttattagtat tatttgtttt
    19801 aatagtaaag ggtataattt ctccagcagg acctatgagt cctgagcata tgatagataa
    19861 aaatctattt ggtgaaggta tatcacaagg ttatcaaaca atggacgctc taggtgcagc
    19921 atctatggca actataatgt tgatgtctat aatagctaaa ggatatacat cagataaaga
    19981 ccaaatagga atgactatta aagctggttt tgtagcctgt gtatttctag cagtagtata
    20041 tggtggtctt acttatttag gtgcaacagt ttcaacttta tatgatacta gtatacctca
    20101 agcatcttta ttagttgaaa taactagtat attgttatat ggtcaagtag gaaaggtaat
    20161 tttaggtgta attgtggcac ttgcctgtct gactacttct acaggtttaa cagcatcaat
    20221 atcaacttat tttgaaggaa taagtaaggc gagttataaa caactggtta taggtatatg
    20281 tgtagctagt atgcttatat caaatttagg tgttgattct ataatagcta tatcagttcc
    20341 aatcctacaa actatatatc ctgttttatt agcaatagtt gttatggagt tggctggtaa
    20401 acatataaag aatgataatg cttttaaagg tgcagcatat gttacattag tgattagtct
    20461 attatcagct ataaatggaa tgacaggagc tgttccattt atacaaagct tgccactcgc
    20521 aggtttgggc tttaactggg taataccagc tatattgggt ggaatagttg gtaactttat
    20581 aaaaagcaac aaacaagtag cataataatt tgtaattaat ttaattttat aatatattaa
    20641 tttttagtac tatgggtagg tttaatctaa acctaatcta cccatagtac tttttagatt
    20701 ggattggaat agcatgtata taatttttta taatataatt tttctttgaa gtatgataaa
    20761 attagtaata gtgataagaa agtacaaatt taggaggttt taattaatgc ttagaaaaat
    20821 aacagcattt atgctatcca tatgtttaat tattggactt acaggttgta gtttacttgt
    20881 aggcaagaaa gataaagaga aaattataga tgattttaaa gttgcagtgg taactcaacc
    20941 attatctgaa aataaagtac aatacaatat ggtggaagag atgacaaaag aatatgagga
    21001 agagaataaa attgataaag acaaagatgg tcaaactaaa gttaaacaaa ctataaaaca
    21061 tgttgtattg ccagagaatt ttacttctaa tatagatagt gctattaata agatagtaaa
    21121 gttggctgac gataaagaag ttcaggctat tgtagtttca actgaccaag cagggctttt
    21181 acctgcttta caaaaggtaa aggaaaagcg tccagaaata ataactattt ctgcccctat
    21241 gggagatgat aaaaaccaat taagccaatt tgttgatgtg aatctagggg ttagtgcaga
    21301 ggaaagagga aaagtattag ctgaacgttc aaaagaaatg ggagcaaaag cttttattca
    21361 ttatgcctct acagatgatt tgaaagatgt taatatagcc aaaagattag aaatgattaa
    21421 agagacttgt aaaaatatag gattgccatt tgtacaagtt aatactccaa atataaatac
    21481 tgaagaagac aaaaacaaag ttaagcagtt tttaaatgaa gatattgaaa aacaagttaa
    21541 aaaatatgga aaagatataa atgtatttgg tgtaaatgag tatatggatg aagttatatt
    21601 gactaaggct ttagaattaa agtatatagt tgcagagcaa agcaatccat caccaataca
    21661 aacgtatcct agtgttatgg gtctaaaaat atctgaaaaa gatgctcaaa attatgataa
    21721 aataaacgac atgataagtg aaaaggctaa ggcttttggt atgtcaaata gacttggtgg
    21781 atatcctatg ccaatggatg cttttctacc atcagtagca atttatcttg ctactgaaat
    21841 ggtgaaacaa gatttaacac aagaagatgt atgtgaccca gattatttag aggcgtttac
    21901 agaattaaga tttggaatcg gttcagaatt tactccttta acagaggtac tttataatta
    21961 tcaatcagta attttaagcc aattaattta ttaatattaa ggaagtgtct caaaatgaac
    22021 tttttagttt atgaggcact atttttctgt taaatcaaat atatttttat ctttttaaca
    22081 attaaaaaga agcttatccc tatgttgaga tagcttcttt tttaatataa ataagtttta
    22141 ttattttata aaaaatttat agaaaaataa aaaattataa ttatatataa attttgataa
    22201 agtaaagatg aattactata ggtttaaatt atattatttt ctattatttt tatgcctttc
    22261 tcagttattg atgagccttt tcttccttta ctagaaatta agtatccatt gcgtgtcaaa
    22321 tattctagca tatttcttac ttcgtattct gtgacattat attccataaa tttcattaac
    22381 tttgtcctcc ccattggttt catgtgcaat gattttagga tagaaatgca tatgtttttg
    22441 tcatattggt ttaaatcatt gaatatagac atattacttg acatatgatt attatttaat
    22501 tttgaagata tgttttgagg taaatcattt attgtaagat aattggaacc catgtaagcc
    22561 atatattcaa cacaatttct cagttctcta atattcccca tccaagtgta tgaatttaaa
    22621 gcttctaaaa gactcttatc gagttttatg tttttatagg gaagttcttt taaaaatgat
    22681 tctaaaagaa gaggtatatc ttctaatctc tttctaagag gaggtaaatc taaagtgaac
    22741 atacttatcc tataaaataa atccatccta aatttatttt ctataattaa tttttctaaa
    22801 tctttatttg tggcagctaa tattctcaca tcaattggaa taatacttgt accaccaatt
    22861 tttctaattt ctttttcttg taagactctt aaaagtttaa cctgtaaatc atgtggcata
    22921 tctcctatct catctaaaaa tatagtgcca gtatgagcta attcgaataa tccttttttt
    22981 ccaccttttt ttgctccagt aaaagaacca tcttcatatc caaatagttc actttcaagt
    23041 aattcacttg gtaatgaagc acaatttatt gctacaaaag gtttattttt tctatttgaa
    23101 tgattgtgta ttgactgagt aaaggcttct ttacctgttc ctgtttcacc tgttattaat
    23161 ataggattat ctgtaagagc catttttttt gcccttttaa tacaatcttt tataatagaa
    23221 ctatttccaa taatattatt aaatttatat ttagctacat aacctctttt gacagaatca
    23281 cttcttattt tttgttctaa tatttctata ttgtttacat cttttaatgt aattaagcaa
    23341 ccctctatat ttttataaag ataaaatggt tttttagaaa gtatgaattt tttatctatt
    23401 tcgtctatgt atataatcat attatcgata tttaagttct ttgtaatttt atctaagaaa
    23461 ctctttggca tatattccat taaggaaggt gattttataa gtttttcatc tagttccaac
    23521 atattaagta aagacttatt tacaaaagta accttgttaa aggtgttgaa aaatattaga
    23581 ccatcaccta tttcatcaat ggtcatgtaa agtatagttt ttaatttaga tatattatct
    23641 aatgacgtat ttaagaaatc atgacttagt gtctccttgg acaatttgta tagtttttct
    23701 ataaatttct cattttttag ttttaatact actaataatg acatatatgt atctaatgag
    23761 atttttctcc aacctaaatc aacaatattt tttatgtatt ttggtataaa tctaatctga
    23821 cctggtgtta tagctgtatc taaatcatat atttcatcac aatctggata tgtcggtaca
    23881 aaatctatat gatttatacc aattttatat agataagtag ccatctcaaa tgctgaagtt
    23941 ttattggttg tcataagtaa cgattttgta cctggaggta gtttcatcat tttttcatat
    24001 ccaagttttg aaagtgcttt cgttccatga ataatttcaa tatcttcctt aaaatgtttt
    24061 attaaaaaat tactagttaa aggtgataga gaaagcacta cctctccctc taaattttct
    24121 tttattccat caagtatatt ttgtgtttct atgattattt cgtctccaaa aaagtttgtt
    24181 aattcttctt tgtagtggtc actatttaat ttatcatatg ttataaggat taacttcttt
    24241 ttatccattt tatttctcct aacaaaatag tatttatttt taaagtattt aaagtataag
    24301 gtaagttata ttatacaaca aatagtagta taaatttata tatttgatta aataataggt
    24361 ttttttagga ttttatccta taaaaaccta ttttaaaatt atttttgcaa gataaggaaa
    24421 atagcttttt atattgtaat gtatatttta aatagttaat aaaaaaatat tatattttta
    24481 aacttgaaaa gtaagtaatt ataagctatt taatcgctgg aataaattta caataaaaaa
    24541 ataaatacaa aggctttatc attaaggtat tggcataagt tttgcttatt aaacaagtat
    24601 actaataaaa ttcatttaaa caggagggag aatttatatg agtaaattag aatcaacaac
    24661 agaaaaagtt gaggtaaaat ggtatgggta tgtagcatta atattgggag cattattttt
    24721 ttcaggaata tttaaagatg caccaggacc attaaaagta ttggatttta acaatgtatt
    24781 aggttctttt ggaacattgg gaacaattaa tgatggtgta ggtacattag cttcaaactt
    24841 tagaggtgat ggaggtactg gccctcgaga tggatggcta tatgcattga cattaatacc
    24901 atcagttatg tttgcacttg gagtagttag agttatagac cacttagatg gaatgaaggc
    24961 agctcaaaag cttcttagtc cactattaaa gccattatta gggttacctg gatttgcagg
    25021 actaactttg atagctagtt tacagtctac agatgcagct gcatctatga ctaaagaact
    25081 taaagatgat gggtatatag atgaaaaaca aaaagctgta ttttgtgcat ttcaattttc
    25141 aggtgcgtct gcaattacga atttctttgc ttctggagca gcactatttc catttatagg
    25201 agatgtacca atatttatac cacttgcttt aatattaatt atgaagtttg ttggggcgaa
    25261 cttacttaga ttatatttaa ataaatttga caagggggaa gcttaacatg agtgaggtag
    25321 caaaaaaaga agtaaaagtt aaaaaaggaa atgtgataga tgcgtttatt ggaggagcta
    25381 gaaatggatt ccaaataagt acaaattcaa tggctccaaa tgtcatcttc ggttttgcta
    25441 taatttctgt gttcaattta acaggtcttt tggatgtaat agggacaata tttacacctg
    25501 taatgagtat ctttggactt ccaggtgttg cagcaacagc tataatgaca atatttatat
    25561 caatgggagg agcatctgga gtaatcgcag gtttatttac agcaggtcaa ataagtcaag
    25621 cacatgttgc aattctattg ccaatgttat tgctttcagg agcgcagata caatttatgg
    25681 ggagactttt gggaacagct gagttaaaaa ctaaatatta tccacatatg tttgttatag
    25741 caactttaaa tggttgtatt gcaatgctta taatgaatta tttcgtagta taggtaatta
    25801 taataaaaat ttaatataaa tggaaagttt aaattttaag gaggagctag attatgggaa
    25861 ctaacaaaga ttatattgag tatgtacata aagtgtatga ttatctacac actataccag
    25921 aaaaaggatt tgaagaatat aagacatcag agtttgtttt aaaagagctt gaaaaaatgg
    25981 ggtttgaaaa tattaataaa ggagttgcag gaactggtat tatagtaact atagattcta
    26041 aaaaagaagg accagtattt gctataagag cagatatgga tgcattggaa tttatcatag
    26101 atggagaaaa gaaaatgata catgcttgtg gtcatgatgg tcatacttct atgcttttaa
    26161 cagctgcaaa gttaataaaa gagagaaatt tagtaaaaag aggtaagtta aaaatacttt
    26221 ttcaaccagg tgaagaaaaa ttatatggag cattaagagt aattgattct ggtttgttgg
    26281 atgatgtaga agaaatggta gggatgcatt taagacctgg acaagattgt aggttgggaa
    26341 aggcgatacc tgcaatgtac cattgtgcta gctgtataat gcaatttacg attaaaggta
    26401 tcacttgtca tggttctaaa ccacatttag gagtaaatgc agtagatgct gtctgtttag
    26461 cagtgaatgc aataaatgca ataagagtaa atccagctat accaaattct ataaaagtaa
    26521 caaatatatc agttaaggga gaaacttata ataatatacc agatgaagca tttatggctt
    26581 tagatataag ggcagaaagt aatgatataa tagaagaatt aaaagaaaaa gcaatacatg
    26641 ctgtaaaaac tgctgtaagt tctataaatg cagaagctgt actaatttcc aaagatggag
    26701 taccagcagc agaatttgat gaagaacttg taaacttggc tgaagaaact ataaaagatg
    26761 tattgggagc tgatgcatct ataggtatat ttaaaaattc tggtggagaa gatttccatt
    26821 attatacaca aaaactaaaa tgtaaaacta catatattgg attaggagct gatgctacac
    26881 caggatttca taatccaaat gtaacatttg atacgaaagc tttagaatat ggtgtagata
    26941 tatggtgtag attggttgaa aagagattgg gataatatcc aaactaataa ttctacattt
    27001 aaaattataa ttatagaagt tatagaaaaa gattgcttat gttaattaga aaataattaa
    27061 tatgagcaat cttattttga tataatgaaa ctgattttgg aatattgaaa aaatatgatg
    27121 gaaaagtaat tatattttaa atctattgga caaaaaagtg atattattat aatattaaga
    27181 attgtaaaat aataccaaag tgcatctagt aatggactta acaattataa aaattttatt
    27241 cttaggaggg aaaaaatgag taagtataat tttgacagag taatagatag agtaggaaca
    27301 gattgtgtaa aatgggattt tagaactaat tgttcaacaa aagcacaaaa agatggtttg
    27361 cctttttgga ttgctgatat ggattttgaa tgtgcagaac caataataga agcattacgt
    27421 aaaaaagttg aacataaaat atttggatat agttcaaatg atagtgataa atactttaat
    27481 gcagtttgtg attggtataa aaggagattt aactgggaaa taaatagaaa agatattatt
    27541 tttagtcctg gtgttgtacc tgctgttgct attttagtaa gaatattaac taattctaat
    27601 gaaggtgtaa tagtccaaaa accagtgtat tacccatttg aagctaagat aaagagcaat
    27661 aatagggaca ttgtaaacaa tcctctaata tatgagaatg gtacttatag gatggattat
    27721 gatgatttag aagaaaaagc taagtgtagc aacaataaag tactgatact ttgtagtcct
    27781 cacaaccctg ttggaagagt ttggagagaa gatgaattaa agaaggttgt agaaatatgt
    27841 aaaaaatatg acctatggat aatagctgat gagatacatt ctgatttagt tagaaaagga
    27901 tttaaacata ctcctttaca gtcattatgc ccagagtaca aggataaaat aattacatgt
    27961 atagctccaa gtaaaacatt taatctggca ggaatgcagt tgtctaatat aatagtgaat
    28021 aatgatgaac taaaaaagaa atatcaagaa gaggttacag ctgttggagt tgcaacttca
    28081 ccaaatccct ttgctattgt agctacaata gcagcatata atgaaagtga agactggctt
    28141 aatgaattaa atgattactt agataataat atacaattta ttgatgaata cttaaaagaa
    28201 aacctaccaa aagtaaaact tgtttatcca gaaggaacat atctagcatg gttagatttt
    28261 actgcttatg gattaaatga agtagaactt gaggatttaa tgtttaaaaa agctaatgta
    28321 ttatttgatg aaggttatat atttggaaaa gaaggaatag gatttgaaag aataaatgta
    28381 gcttgtccac agtctttatt aaaagaatgt atggacagat taaagactat atttgaaaat
    28441 ttataaagta taggtaaaaa tttataaagt gtttaaaaat aaaaaaataa taaacttgac
    28501 aaattttaac taatcactta tcatactatt atgaataaat agaaaacata agagagctaa
    28561 attttattga attaaactct taaagacttt gaaaggaaga gtagttatag aatgtaatta
    28621 aagcgagttg gtgatggtgt gagaccaata tgaagtctat aatgaagaac atcctggagt
    28681 tgctaaccga aattttaaaa gagtaggctt agtcggaacc aaaccgttat catattgggg
    28741 tgtatcgagt aatatctgta cattgtgagc tgggtttgaa tttatttcaa actaatcagg
    28801 gtggtaccgc ggaaaagcaa cctttcgtcc cttttatata gggatgaagg gtttttttaa
    28861 tggaaaaata tataaatata gggagtgaat gatatgagca caataatacc aaaggattat
    28921 aaaagtagtt tgaacgtaat tgatacacaa atagctataa aaaaattaaa agattttttt
    28981 gaaatgagac taagtaatga attaaatctt ataagagttt cttctccgtt atttgtattg
    29041 ccagaaacag gtgcaaatga taatttaaat ggagaaaagg ctgtaagctt tgatatacca
    29101 tttatgaata aaaatgcaga aattgttcaa tcacttgcaa aatggaaaag attagccctt
    29161 aaaaaatatg ggtttgaagt tggtagtggt ttatatacag atatgaatgc aataagaaaa
    29221 aatgaagaat tagacaatat acattctctt tatgtagacc aatgggattg ggaattggtt
    29281 atagataaaa gtagtagaaa tgaaaagact ctaaaagatg ttgtaaaaag attgtatgga
    29341 gtatttaaag atactgaaat ttttgtatgt agtatgtatg aaggtataaa agaaatatta
    29401 ccagaagaaa taacttttat aacatcacaa gagttagaag atatgtatcc agaattatca
    29461 ccgaaggaaa gagaagataa aattgtaaaa gagaagaaag cagtatttat aactcaaata
    29521 ggaaaaacat taatatcagg agaaaaacat gatggaagaa gccctgatta tgatgattgg
    29581 gaattgaatg gggatatatt attttacaat cctgttttag attcagcatt agaattatct
    29641 tcaatgggaa ttagagttga tgaagaatct cttgatagac aattgaaact tgctggatgt
    29701 gaagagagaa aagagtttga ctatcataag atgcttttaa atggcgaact tccgtataca
    29761 attggtggag gaattggtca gtcaagaata tgtatgtatt tcttacaaaa agctcatata
    29821 ggagaagtac aagttggagt atggccacag gatatgattc aaaattgtag tagtgcagga
    29881 attgaattat tataatttaa atataaacta attgataaaa tttatatcta atatgttcat
    29941 caaattaatt atatcggtta taatgtagaa taacctataa ttataagagg tgataaatat
    30001 ggagttttta aaaataatca ttaatatagt tttagatatt ttaaaaaaaa ttttggtcag
    30061 atttaaaaat gctaaatttg gtttggtttt tgtatttgat ttacttaaac tacctgattt
    30121 tatgacagat aaaagaatca atatagttga taaaataaag gtaatttctg tattaatttt
    30181 tactatatcc tactttgtat ctggggttga tataattcca gagatgattg caggggcatt
    30241 tggttttata gatgatgcta tagttttaat ttggagtata ggtatagtga atgaagaaat
    30301 taataagtat agagttatta taaaaaaaga taaacattca aatataattg aaaatgtaga
    30361 attttctata aaagatgaag aagaataaca atttatatat gttattcttt tttttattaa
    30421 caaaaaatgg ttaaattgtt gacggagagg taaaactcta ttatcataat aagtggatat
    30481 ccaatattta actaagtatt caatgtgcaa gaatgcttaa tttaaaaaag ttatggaggg
    30541 taaatattat gaagaaaaaa ataggtttag tgcccaaact aatcatcggt atcatagtcg
    30601 gtattttaat cggaagttat gcaccagaaa taatagtaca aatattagta acagttagta
    30661 ccttattctc agcattccta aaatttgtta taccatttat aattataggt tttgtaacag
    30721 caggtatagc tgatttagca acaggtgcag gaaagttatt ggggataact acgggaatag
    30781 catatggttc taccttaata gcaggattat tatcatttgc agtatctaca ttaatcttcc
    30841 caaattttat tgatgcaagt gttgcaagtc aaattggaga cccagaggca ggaatgttag
    30901 ctccaatatt cacaattcca ttagagccta tggtagatgt aacagctgca atcgtatttg
    30961 catttgtaat gggacttgga atatcagctt taagaaatca tggaaaagga gagactttat
    31021 ttaatttctt ccaagaattt caaggaatcg taacgaaaac gttgtcaaca gtgattatac
    31081 ctttattacc tttatacata gcaggaactt ttgcaaatat aactatagca ggggaagtat
    31141 ggacaatatt aggggtattc tggaaagtat acttagtagt aataccttta cattttgtat
    31201 atatggcatg tcaattcaca gcagcaggtg tattttcagg aaaaaatcca gtaagaatgt
    31261 taaaaaatca agtacctgga tatttaacag ctataggaac tcaatcttca gcagcaacta
    31321 taccagttaa tgttggttgt gcagaaaaaa atggagtaag taagcaaata agagaatttg
    31381 ttgtacctct ttgtgcaaca atacatttat caggtagtat gatatcaata acaggattta
    31441 caactgcagt tttaatgatg aatggtatgc cacatggaat aggtgtatta ttcccttata
    31501 ttgcaatgtt aggtatagca atggttgcag caccaggagc accaggtggg gctataatga
    31561 gtgcacttcc tttcctacca atggttggaa taccatcaga tggtgggctt gcaagtttaa
    31621 tgatagcttt atacttaact caagacagtt ttggtacagc atgtaatgta tcaggagata
    31681 atgcaatagc agtagtagta gataaaataa atgatggaat gagtaaaaaa ggaaacaaag
    31741 aagagcaaag tgcttagttt tttaataatc aaaaatgagt tatctcaaat agagatagct
    31801 cattttttat ggtataaaaa aattataaaa taaagggatt tttttattta aaaattgctt
    31861 taagagatga tattaataat ttattttcat taggttttaa tatacatact ctaaagaaat
    31921 actcatctag accttcaaat gaacaacaat ctcttattat tattttttgt ggaagcagtt
    31981 gttctcgaag cgatttagca gttaattcct ttgttttgat tttacacaat atgaaattac
    32041 ctttagtatt gtatatatct aagcttttta tattcttaag ctctttaagc aggtaatctc
    32101 tttcagtgtt cattaaggaa atagtatttg atataaaatc taaatcacta aacatgattt
    32161 cccccatttt agaagctatt atgtttatat tccataaatc tagattttta tttatttcat
    32221 ttttgacatt agtgttagat ataagaccat atccaagcct tattccaggc gttgaaaaaa
    32281 attttgatgt acctcttata acgaataaat tagaataatc atcaactaat tgagtacaag
    32341 agtatgtatc tgtatcagta aactctacat aagtctcatc aatcattaaa aatgagtctg
    32401 tattttttaa aatttctctc acttctactt tagtaaatgc aaatccagtt ggattactag
    32461 gattacagat tactaccaaa tcataatctt tagcattgat agtttttata agattttcta
    32521 aatttatatg aaaattatct tcttcttttg caaagtattt ttctatagaa caattaattt
    32581 tagataactc tttttcatac tctgaatagg ctggagagag taaaagagcc tgtttaggat
    32641 taattgtatg tataaatgat gaaattagtt ctgtagctcc actgccaagt actatattat
    32701 ctatagaaca tttgcagtaa ttggatatag aagtttttaa gtctatgtaa tcagggtcag
    32761 gatacataga taccatatct atattgttta caatgtattg tttggctaaa cttgatgtac
    32821 caaaaggatt tatatttgag ctaaaatcca taaattcttc ctttgaaaag ccatacttac
    32881 tagataaact atataaatta gctccatgat ttgtgctcat aaaagtacct ccaaaaataa
    32941 aattatataa tcgataaaaa taactgatta atatatgtac atatagatta taatacaaag
    33001 gaatcgttat tccaaatttt taatggaaaa agcaaaaaag tatggataag ctgttaattt
    33061 aggtctaaaa atgatgaaaa agattttaat tgatattttg gtatgctata atatataagt
    33121 taatagaagg aaagcgggag gatacaaaat gtacaaagtt acaggaaaaa tgttgacaga
    33181 agaacaaata aaagaaaaag tttatgaatt aggaaaaaag atagaagaag actttaaagg
    33241 cgaagattta ctagtggtag gtatacttaa aggagctagt gtatttgttt cagatttaat
    33301 aagatgtata gatttggatg taaatataga ttttatgagt gttacaagtt atggaaactc
    33361 aacagagtct tctggaacag ttaaaatatt aaaagattta gatgtagata ttgaaggaaa
    33421 gaatgtgttg attgtagagg atataataga ttctggttta acattaagta atctagttgc
    33481 agctttaaaa actagaaatc caaaatcttt aaaactatgt actctacttg acaaacctca
    33541 gagaagaaaa gctaatatac ctgtagacta tgttggattt gttatagaag acaaatttat
    33601 tgttggttat ggaatagact atgctgaaaa gtatagaaat ttaccttata taggtatagt
    33661 tgaggatgta gaatagattt aaatgtgaat caaaagcact aatttctaag ggagttagtg
    33721 ctttttagtt aattatataa aattttagta aaggagacat ctatatggat attcagttaa
    33781 atatattatt atttttccaa aatttaagaa atccaatttt gaactttata tttcttgtct
    33841 ttacaataag cacagaagca ccgttattaa tattaattac aacaattata tactggtgta
    33901 taaataaaaa atgtggacag aaaatactgt ttgctataat tggaaatttt gttgtaaatt
    33961 taggcatcaa agagtttgta aaagctccca gaccaatagg aataaaaggt cttgaatcat
    34021 tgagagtttc tactgctggt ggttattctt ttccaagtgc tcatactcaa actgcaacaa
    34081 cattttgggt tagtattatg actatattta aaaagagata tcttcattta ataggtgcag
    34141 ttatggtttt aggagtagga ttgtctaggc tgtatttagc tgttcattgg ccaatagatg
    34201 tgatagtggg ttggatgtta ggaatatttt ttacagttat atttataaaa atatttgact
    34261 atattgatga taataaaaat tatattttac ttgtagtttt acttatacca tttataatag
    34321 tagctatatt tctaaatagc cctgaatatt ttgaaaaatt tggtataata gtaggatttg
    34381 tatttggata tatggttgag gatagatttg ttaaatttaa tacagataat aataacaaaa
    34441 aaataaattt tagcaataaa aataagggga gtaaaaatat aattttttca agaatatgta
    34501 ggtttttggt tgggatagta acaataggaa tgttatatat tggcattaag ttgtttattt
    34561 caatgttaat agtcacttta aatataagtg actctactat gagtataatt tttatgaatt
    34621 ttattaagaa tacagtagtt gttttttatg gaatagcagg agctccagca ttatttaaac
    34681 ttttaaaatt aaattgattt taaggtatag agtcaatagt ttaaattaaa aaaattaagg
    34741 gtagaaaaga gtgattattt gaagaaggta gtaataatag gtggtggacc agcagggatg
    34801 atagccgcat caactgcatg tgagaaaggc tatgatgtaa cattaattga gaaaaatcat
    34861 aaattaggaa aaaaacttgc aataacagga aaaggtagat gtaatattac taacgcttgt
    34921 gaaatagagg aattaataga aaatgtacct acaaatggaa aatttcttta tagtgctttt
    34981 tatactttta caaatgatga tgtaataagt atgtttaata atcttggagt aaagactaaa
    35041 acagaaagag gaaaaagggt ttttccagag agtgataaag cctttgatat tgtaaatgct
    35101 cttgaaagac aacttaaaag taagaaagta aatatattat taaattcaaa agtagaaaaa
    35161 ataatatcta aaaataataa aattgagaaa gtaattttaa acgataaaaa agaaattaag
    35221 tgtgattcag ttgtagtagc aactggtgga ttaagttatc cacttactgg ttctactggt
    35281 gatggatata aatttgcaat ttcacagggg cacacaatta tagatacaaa accatcttta
    35341 attggtatag aagtacaaga aagttttaca aaagatttag aaaaattatc attgagaaat
    35401 gttgaaataa gggtgtttaa tagcaaacaa aagaaagttt atagtgattt tggagaactt
    35461 gaatttacta gatttggttt agatggacct ataatcaagt ctgcaagttg tagaatgaaa
    35521 gatacaagga aagaaaatta cactatatta ttagatttaa aacctgcatt agatgaggaa
    35581 aaactggata aaagagttca aaaagatttt cagaagtata caaataaaaa atttgaaaaa
    35641 gcattagatg atttacttcc aaaaaaacta atacctataa taataaactt aagtgaaatt
    35701 aatgcaaata cggttgttca ccaaatttct agagagcaga gaaaaaactt agtccattta
    35761 ttaaaaaatt taaagtttac agtaaagaga tacagaccta ttgaagaagc tataataact
    35821 tctggtggtg taaaagtaaa cgaaataaat tcaagcacaa tggaatcaaa attagtagaa
    35881 ggtttatttt ttgctggaga agtaatagat atagatgcat atacaggtgg attcaatttg
    35941 caaatagctt tttctactgg atatttggca ggttttaatt gctaaaaatt acctgaatta
    36001 aaaatgttta aaatcacata atattatcag caacacaaaa taaattaaac agaaggaagg
    36061 taatcaacat ggcaagtaac aataacaaca acagaacagt agtaccagga gcaaaagagg
    36121 ctttaaacca aatgaaatta gaaatagcta atgaacttgg tatgagtaat tatcaacaag
    36181 ttgataaagg aaaccttact gcaagagaaa atggttatgt aggtggatac atgactaaaa
    36241 aattagttga aatggctgaa caacaaatgg ctggaaaaag caacagatag ttataaataa
    36301 ttatttataa ctattggcta ttagtttagc tagagctatc tcaaatatag aaagtaattt
    36361 ctaaattgag atagcttttt tttattaaat tattaaatag atatattttt atgtaataaa
    36421 ttttatgtat ttggataaaa taatacaaat gaatatgtta cataaggagt gatgtaaaat
    36481 gagtagtgta aattttgagg atttaaaaaa caaatttatt aatagtgact tagatgaaaa
    36541 aataagaata tatacaacaa cagaaggttt atcagtagaa caatttaaag agctactaaa
    36601 atattatcca atacaacatt tatctaaact agaaaaagca cttggatagc agtatttagt
    36661 ccaaaattaa aaaggcaatt tatttaatat taaaaatatt ttttattaaa taaattgcct
    36721 ttttataata ggtttaaatt attatgcttt tgatatataa ttatagattc tctcatttcc
    36781 aagttcaata gacccagtat taataagagt aacgattcct tctatatctc tagcagttaa
    36841 gtatttatat ggaattaata aattataacc agtattactt agtaagtttt ttaattgggt
    36901 tatttgagag atattgaaac attttgcagt tttagtagat ttttttctta agttagataa
    36961 attttcattt tgaatattta ttaaagtttc tatatagttt ctataagata tcttaaaatt
    37021 tgtctcattg actatttttt cattaagtat actactgata aaatgttgag acaatatatt
    37081 atctgcaaat ttatctgctg ttaattttcc agatgttatg cctttttgat attttctaag
    37141 aattttttta gtatagcagt atattttatt tctcgtttct aaatcgagtt gttctatttg
    37201 cacaaaacca cttccttcta attataaaaa tatttattaa aaatctatac ttaactaata
    37261 aagcttcaat aatttattta cagtaaatac taaatcataa aatatttgtt attttttatt
    37321 attaccaagt ataaaaaata ttataattgt aaaacttaca aatatatgta aaatatttag
    37381 gagtgatgaa aatgttaaat gggataataa agctttttta ttctgaaatt gtaaaaacaa
    37441 agattaataa cctagactca agtggatatt ctttaagaga tgaatgtaaa gaaatcagaa
    37501 atataactct aaagatagat agcgtatttt ttaagctttt aaagttggct tttggttcaa
    37561 tactgcttat gatagctttt aatttaaaca tgattctagg tataggagtg tttttaattg
    37621 gtgtagttta tatactatat aaaagaaata tggaagaaca agtaaaagag catataaata
    37681 atgtgaaaaa taatatcgac atctctaaaa taacttttat ggatgaaaaa gggagaacag
    37741 gtctaaatgc attgattaca ttacttctta taggattatt gacaggattt aactggatat
    37801 tagttgctag ttttgtcgtt gtttttgttt ttactataaa gaatgtatgt taaaatacta
    37861 ataaatagtt taatatatag ggggagaaat tgagtatgac taatggcagt tataaaatga
    37921 agaatttagt ggagatatcc gctatggtga cggaatctac agattttttt aatataaaag
    37981 atgacataat agaaaaaatg ttagaagttg ttcaccctac aaaggcatgt gtaaatctat
    38041 tttataaaaa tgattctaaa tatgcctact tagtatgttc gcaaacctta gaatatgtac
    38101 ctcaaatttt tccaataaat agtcttaaag gtgcaaaaat tgattttgac acatatccag
    38161 agtatataca tgaggctgta aatgaaaaaa agataatata tgtggaaaat gtatttgaag
    38221 attctagagc agaaggtgaa cgagatttag caaaagctga aggttatatt gggagaatag
    38281 tttttccttt tataataaat aatgtagttg ttggatttat gacttgtttt ttaaaagagg
    38341 atgattatat aacagaacaa gatatagatt ttatatcatc tgttgcatca ctcttaagtt
    38401 tatcaataga aataactaat aaaaataata atgtacaaat tttaattgat aaactaagag
    38461 gctcaataag tgccataaat gaagctacta aaaaactata ccttaataag agtattgatg
    38521 gctttttgga aaatttaagt aaacaagcgt gtaatattac taatagtaaa gaagctttaa
    38581 taattataaa tagtcatcat aataaggata ctatagttag ttgttataat gttgagcaaa
    38641 aaaagaaaac aaatttatat cctatgattg atatgttttt aaagaaagag tcattagggg
    38701 gatacctaaa taatttaaaa ccatgctcaa aaaagggcat aaatttggaa agttatatat
    38761 attacaaact tcaggataaa aatgagacta taggatgtat agtttgtgca aatagtaaaa
    38821 actatactag tgatgattta aatattctta gtatattgtc caaacaagtt tctgttggga
    38881 tgcaattata tgaacataat gaaaaggaag taaaacacaa agttcttgaa aatgagttga
    38941 acatattaaa taaacaacaa cagcttatta tggataaagg taaaatggat tgtaatgatg
    39001 agaaagaact ttatttttac cataaaccag caagggtagt aggtggagat ttttattatg
    39061 ctacaaaagt ggatgacgaa catatagttt atattgttgc tgatgtaatg ggacatggaa
    39121 tggtatcaaa ttatgttgtt gcaatgataa aaggtgcttt taaagtatta tgcaatcaat
    39181 acaatacacc aagagaaata atgacaaatt taaataggat gttatatgat gaatttgaca
    39241 agatgggagt atttacaact tgtttgatag gtattataga tactaaagaa aatatgatta
    39301 ctgtgtctaa tgcaggtcac tatagtcctg tagttgtaaa gaaagatggc acaatagaaa
    39361 ataatttaaa ttgtaaaaag ggcataccta ttggaataat gaaagatgct acttatgaaa
    39421 ataatactct ttcaatggaa gattatgcaa tggtatgtat gtacactgat ggaattttag
    39481 agataaaaaa ttcatcaaaa gaagagtatg gtataaacag attagaaaac tttctaaaag
    39541 aaaattttag attaaatcaa cagttaatag tagaaaatct aaaattggat ttaaagaatt
    39601 tctcatcaaa agataattat gatgatgata ttttaatagt tatgttaaag gataggtaga
    39661 ttgattatgg attcaaacga taaaaaagga cttaaaagta cagagattat aataataaat
    39721 ggaagtccaa gaattaataa aaattgcagt tttatagcaa ataaagtaaa agctatgcta
    39781 gaagagtgtg ggttaagttg taaaatattt aatatctaca atatggatat tgaatattgc
    39841 acagcatgtg gattttgtga aaaaacaggg tattgcaagt ttaatgatga tatgaagcca
    39901 ttatataaaa tgtttgatga ttcaaaagga actatagtaa taagtcctgt acattttgat
    39961 tcaatatcag ctaaattaaa gactgttgtt gatagaactc aagctatata tgcaagtaaa
    40021 tatgtattaa ataaaccttc aattgataga aataagaaac ggataggaat gtatattgca
    40081 gtggggggga gtaaatcata tgattcacag tttaaaggtg gacagattgt tatggatttt
    40141 ttctttaaga gtataaatac taaactttta cacaatatat atttaaacaa tacagatgat
    40201 gtatcttata tagataatca agaatttaaa gatataatat ataaaaattt aaagatatat
    40261 attgattcaa tataatttga ttttgtaggt aatattatgt ataattgtat tgcaataatt
    40321 taaataatta aaaggaggga atttcaatga aattaccaat agcattatct aataaacatg
    40381 ttcatttgag tcaagctgat atagacgtat tatttggggc gggtcatgag ttaactaaat
    40441 ttaaaccatt atctcaacca gggcaatatg cctgtgaaga aaaagtggat ttagtaggac
    40501 caaaaggaac tttaaaggga gttagagtac taggaccagc tagagctcat actcaagttg
    40561 aaatatcttt agcagatggt ttcccattag gagtaaaagc accaataaaa gaatctggtg
    40621 atatagctgg aactccaggt gttaaattag taggacctgc tggagaagtt gaaatgaaag
    40681 aaggagttat agttgcttct agacatatac atatgagcaa tgaagatgct gctaaatttg
    40741 gtgtaactga taagcaaata gtaaaagtta aaacatctgg accaagagca ttagtatttg
    40801 aaaatgtatt agtaagagct agtgatagct ttaatctaga aatgcatgtt gatatggaag
    40861 agggaaatgc agcaggcgtt aaaaatggag atttagtaga attaatagtt gattaatatt
    40921 ataaatagac tttatgctag tagatatttt atttgctagc atatttttaa atgtaataaa
    40981 tttctatagg ataaatttaa tcgattagat taatttataa aataaagggg tattataaaa
    41041 aatgtaataa aaacttacaa aaatatgatg taatatggta aaattatata tataaaaatt
    41101 tacatataat aaagataatt attttacttt ttatactaa
//
LOCUS       DAEPOG010000013        41086 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEPOG010000013 DAEPOG010000000
VERSION     DAEPOG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.013"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..491)
                     /locus_tag="KR432_000606"
     CDS             complement(<1..491)
                     /locus_tag="KR432_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0639161.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKI"
     gene            complement(583..1338)
                     /locus_tag="KR432_000607"
     CDS             complement(583..1338)
                     /locus_tag="KR432_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0639162.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            complement(1335..2291)
                     /locus_tag="KR432_000608"
     CDS             complement(1335..2291)
                     /locus_tag="KR432_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0639163.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            complement(2281..3234)
                     /locus_tag="KR432_000609"
     CDS             complement(2281..3234)
                     /locus_tag="KR432_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0639164.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            complement(3481..4461)
                     /locus_tag="KR432_000610"
     CDS             complement(3481..4461)
                     /locus_tag="KR432_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0639165.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            complement(4518..5720)
                     /locus_tag="KR432_000611"
     CDS             complement(4518..5720)
                     /locus_tag="KR432_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBH0639166.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            5915..6469
                     /locus_tag="KR432_000612"
     CDS             5915..6469
                     /locus_tag="KR432_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0639167.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            complement(6584..7375)
                     /locus_tag="KR432_000613"
     CDS             complement(6584..7375)
                     /locus_tag="KR432_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBH0639168.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(7403..8491)
                     /locus_tag="KR432_000614"
     CDS             complement(7403..8491)
                     /locus_tag="KR432_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBH0639169.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            complement(8545..9783)
                     /locus_tag="KR432_000615"
     CDS             complement(8545..9783)
                     /locus_tag="KR432_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBH0639170.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            complement(9761..10837)
                     /locus_tag="KR432_000616"
     CDS             complement(9761..10837)
                     /locus_tag="KR432_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBH0639171.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            complement(10901..12037)
                     /locus_tag="KR432_000617"
     CDS             complement(10901..12037)
                     /locus_tag="KR432_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0639172.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(12038..13600)
                     /locus_tag="KR432_000618"
     CDS             complement(12038..13600)
                     /locus_tag="KR432_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBH0639173.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(13687..16446)
                     /locus_tag="KR432_000619"
     CDS             complement(13687..16446)
                     /locus_tag="KR432_000619"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0639174.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(16436..17065)
                     /locus_tag="KR432_000620"
     CDS             complement(16436..17065)
                     /locus_tag="KR432_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0639175.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(17151..17729)
                     /locus_tag="KR432_000621"
     CDS             complement(17151..17729)
                     /locus_tag="KR432_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0639176.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(17768..18346)
                     /locus_tag="KR432_000622"
     CDS             complement(17768..18346)
                     /locus_tag="KR432_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0639177.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            18675..20111
                     /locus_tag="KR432_000623"
     CDS             18675..20111
                     /locus_tag="KR432_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0639178.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            complement(20203..22077)
                     /gene="asnB"
                     /locus_tag="KR432_000624"
     CDS             complement(20203..22077)
                     /gene="asnB"
                     /locus_tag="KR432_000624"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0639179.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            22280..22954
                     /locus_tag="KR432_000625"
     CDS             22280..22954
                     /locus_tag="KR432_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH0639180.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            23067..23603
                     /locus_tag="KR432_000626"
     CDS             23067..23603
                     /locus_tag="KR432_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0639181.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(23769..24389)
                     /locus_tag="KR432_000627"
     CDS             complement(23769..24389)
                     /locus_tag="KR432_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0639182.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(24703..26841)
                     /gene="vanT"
                     /locus_tag="KR432_000628"
     CDS             complement(24703..26841)
                     /gene="vanT"
                     /locus_tag="KR432_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0639183.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(26859..27665)
                     /locus_tag="KR432_000629"
     CDS             complement(26859..27665)
                     /locus_tag="KR432_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0639184.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(27662..28762)
                     /gene="vanG"
                     /locus_tag="KR432_000630"
     CDS             complement(27662..28762)
                     /gene="vanG"
                     /locus_tag="KR432_000630"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0639185.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(29019..30101)
                     /locus_tag="KR432_000631"
     CDS             complement(29019..30101)
                     /locus_tag="KR432_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0639186.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(30151..30852)
                     /gene="vanR"
                     /locus_tag="KR432_000632"
     CDS             complement(30151..30852)
                     /gene="vanR"
                     /locus_tag="KR432_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0639187.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(31286..33817)
                     /locus_tag="KR432_000633"
     CDS             complement(31286..33817)
                     /locus_tag="KR432_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0639188.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(33925..34542)
                     /locus_tag="KR432_000634"
     CDS             complement(33925..34542)
                     /locus_tag="KR432_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBH0639189.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(34904..35788)
                     /locus_tag="KR432_000635"
     CDS             complement(34904..35788)
                     /locus_tag="KR432_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0639190.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(35979..37229)
                     /locus_tag="KR432_000636"
     CDS             complement(35979..37229)
                     /locus_tag="KR432_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639191.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(37451..38215)
                     /locus_tag="KR432_000637"
     CDS             complement(37451..38215)
                     /locus_tag="KR432_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639192.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(38209..38934)
                     /locus_tag="KR432_000638"
     CDS             complement(38209..38934)
                     /locus_tag="KR432_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639193.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(38936..39304)
                     /locus_tag="KR432_000639"
     CDS             complement(38936..39304)
                     /locus_tag="KR432_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639194.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(39480..41024)
                     /locus_tag="KR432_000640"
     CDS             complement(39480..41024)
                     /locus_tag="KR432_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0639195.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT        13807 a         7132 c         4237 g        15910 t
ORIGIN      
        1 aatatttttg ctattttgtc tatattattt ttaacagatt ctaagtactt cttatcatct
       61 ttacttgtac ttattgttgg tgctattttt gacaattgtt cataaaaatc ttcttgtctt
      121 ccatttatta taattaaatc tggatttgcc gactttatac cttctaaatc tggttctttt
      181 aaacctccta aatctgcata cttcttatcc ttatattttt ctaaagatgc tggtaaactc
      241 gtttttggaa gtcctactaa attctcagct acacctaatg catccattgt atctaaagcc
      301 gaataatcaa atactactac ttttttagga tttaatttta catctgtttc tcccaagtta
      361 tgagttatct ttatggtatt atttgaattt tctgatgttt ttgattcatt ctttttactt
      421 ccacctaatg caaacactgt cactaatcct ataatagcta ctgctgctac tattgctgct
      481 tttttattca tatcaatatt ttcctctctc ctattaatct ttattttaat ttaaaaactt
      541 tataaaaaat catatagcaa gcttattttc atatacaata ttttaaaaat aaatacagat
      601 tcgatttcca tttatattct taatgttaaa attcatctca taaatatctt ctaaaacttt
      661 ttcatttact atctcatctg tacgacctac tttttcaact tttccatttt ttaatgcaac
      721 tatattatct gaataacatg atgcaaaatt tatatcatgc attactaata ctatagtctt
      781 atctaactca tcacaaagac ttctaagtac cttcatcatc tgtacggaat ggttcatatc
      841 caaattgttc agtggttcat caagtaatat gtactctgta tcttgagcta ttaccattgc
      901 tatataagct ctttgtcttt gacctccact caattcatct aaatacttat gttgtatatc
      961 agtcaacttc atatattcta ttgcttcatc aatataattt tcatcctctt tagttagatt
     1021 accttcacta tatggaaatc ttccaaatcc aactaattct cttattgtaa gctttaaagt
     1081 tatattgtta gattgtttaa gaatagctat tttttttgct aattctttat tgctataatt
     1141 agtcaagctt tttccttcta ttattacttc tccgccatca cctcctataa gccttgtaac
     1201 tattgatagt actgtacttt ttccagctcc atttggtcct ataaaagatg ttatctttcc
     1261 cttttctata ctaaaagaga catcatctac aacattttta ttcttatatc ttttaaatat
     1321 attttttatc tctatcatct atttgccccc tttaatagta attgaataaa gtaaattcct
     1381 cctataaaat ttattactac acttaaagtc gtatcaaact taaatacatg ttgtataaaa
     1441 aattgaccta ttatcaatgt aatcatccct atcaacatag aacccgaaat caagtatgta
     1501 tgcttatatg ttttaaaaat ttctcttgtt atattagcaa gtaaaagtcc taaaaatgtc
     1561 ataggtccaa taagtgctgt tgatattgag actaaaattg ctattaatat tatcattttt
     1621 ttaactgcct tatcaaaatc tacacctaaa ttaattgctt gttctttacc taacgacaat
     1681 acatctaaat actttatttc atcatatata aaaggtatta tggcaataat tattataaat
     1741 gctattaaca atacattagt gtttatattg tttaaacttg ccattatact agtttgtaaa
     1801 gctaaaaatt cattcgggtc tatcattact tgaataaatg ttgttgcact tttaaataag
     1861 gttccaaata tcatacctac taggattaaa aagaaaatat tatttttatt tttttcaaat
     1921 aacatcttat ataataacaa tgaagctcca atcatcatag ctacattaat aataaaatta
     1981 taattttcat ttataatcag actactgctt gaaccaaata tgaatactat tatagtttga
     2041 atcattacat acaatgaatc taaacctagt acacttggtg ttaatatttg attgtttgta
     2101 attgtttgaa acactacagt agtaaatgct atacatcctc cacctaatac catagcaaat
     2161 atttttggta ttctttgtga taatgcatat tctaaatgat tcatgtctat tccataaatt
     2221 ataaaaagtg ctattgatat gaatagtaat atacttacaa agtaaatttt ttgattgaga
     2281 ctaagcttca ttcacattcc tccttaatat taaataaagg aatagtatac ttcctataac
     2341 tccaactgta agtcctatag atatttcata tggataaata ataattcttc caaaaatatc
     2401 acatataagt acaaataccg ctccaagtaa accagtatac catatgcttg ctcttatatt
     2461 atcacccata tacaaggata cgatattggg aactattaga cctataaaag ggatattacc
     2521 agctgtaacg actacacata ctgtaacaag tgctactatt atcagaccta tattgacaat
     2581 tcttttgtaa ctaagaccta agtttgtcgc aaaatccata cccataccaa caacagtaaa
     2641 tttatttgca taaatatatg ctaatattat aaggggtatt gtgatatata atatctcata
     2701 atttccttta agtatcatag aaaagtcacc ttgcatccaa gaactaacat tttgactcaa
     2761 atcgtattta tacgcgataa aatccgtcat agaacctatt atatttccaa acattatacc
     2821 tactaatggt acaaagatta tatttttaaa ttgtaacttc cctattattt tcataaacgt
     2881 aaaagtccct acaagagaaa atacaaatgc tattattgtc tttgtaaaaa tagaggcagt
     2941 tggtactaaa agcatacata ttactatccc aaattgagct gcatctattg tagcacctgt
     3001 tgtaggtgat acaaatttat tcttacttat ctgttgcatt ataagacctg ctatgctcat
     3061 tcctacacca gcaatcaata tacttattaa tcttggtacc ctactcatga taaatatcat
     3121 aagcttatgc tgattttttg caaatatatg cattatattt atatcttcag ctcctataaa
     3181 taaagaaaaa taagacaaaa ttattattcc tatgactaaa taccttttct tcattctccc
     3241 tccttaaaaa tcttttatta attaaaatga aaattattat cattttcact tttatagttt
     3301 atcaataata attatcattg tcaatatata tctatatcta tttttaacat aaaaaatgtt
     3361 actagaattg aaacttctag taacattaaa atttaattta ataaattatg aacgccaatt
     3421 taaatatgta gatattattg tataaatata ctattttata aatttaagtt ctttattata
     3481 ttattttagt atatctttaa agagcctata aaaattacca ctagagatta actctatatc
     3541 tttcttttta taacctctat tttcaagctc tactactatg ttctgtgctg tatgtgtgct
     3601 ttcaagacca tctacacttg gcacactagc atttccagta aaagaaccta tagtctcttt
     3661 ccctaagtaa tcactaaaat caaaaccaaa agatacatgt tctataccta taagatttac
     3721 catatagtct atatggtcaa ccaaatgtat taaatcttgc ttcttaggat tactattagt
     3781 aaatcctcta aaactattta ttccaactaa tccacctgta tttgaaattt cttttaattg
     3841 attatctgta agatttcttt tagcatcaca taaactccta gcattagaat gagaagctat
     3901 aactggctta cttgcaacag acattaaatc ccaaaatgat ttttcattta aatgtgacac
     3961 atctactatc atgcccaatt tttccatttt attgactgct tttttaccta tttcagttaa
     4021 accacgattt tcatccccta aacatcctgt agcaagttca ttctgttcat tccaagttaa
     4081 tgatgcatgt cttgcaccaa cttctttata aaaataatct agtaaatcta tatttcttcc
     4141 aatatggctt aatccttcaa gaccaattaa aatatttatt ttgttttcac ttataccttt
     4201 atctatatct gaatattttt ttactatgtt tacaacatct ttcatatctt caatttcatc
     4261 ttttatagag cttataattt gtttaactct tttttcaggc tctttatcat atggtgggtc
     4321 tatccatatt acaaaaatag ctccttctat atttgctctt ttaaaattat caagatggat
     4381 tcttctaaat acatcttttt ccccgttaag ccttcttatt gttacatctg cccaagtatc
     4441 tgaatgtcca tcgaaaatca taacaacctc caattaaata aaaatctttc taaaaaacaa
     4501 cttcttttca cgtaaaatta tatcaaaata tttcctacta aaagaccaaa ataattacca
     4561 actatgtatc caaatgttcc aactataagt attggcccta ctagcttaga ccatcctttt
     4621 gatattgcca tagctgctgc tgttgtagga ccaccaatat ttgcattaga tgcaagaata
     4681 gcttcttcta aactaaactt aaatattttt gcaactataa atgtaactag catgtttatt
     4741 agtaccatta ttccgcagta taaaagtagt aatggtgatt tttgaattat taatgttatt
     4801 gaagcaggaa cacctactac aacaaagaat atatatatta aaaatgtacc caattcctgt
     4861 gctcctttta tatcaccaaa gaaattaggc ataaatgtgg caaacaacat agtcaaagta
     4921 gttattatta gatattgatt tccaaataaa gtatttaaca ttgctaaaaa aggatttgat
     4981 aatggtatta tagctttaaa ccaagttgat agttctacag atactgcgac aattataaag
     5041 gcgctaccaa tggctattgc tatatctttt aaagatattt cttttcttcc ccaataattt
     5101 gctgctactg tttctccttc agttatacct atactttcca tttcatctac atgtggatgt
     5161 ttaaaatgtt ttctaaaaaa tccaattgat ggtatagcta ttaagacaaa gaaatataat
     5221 gccattaaaa gattatctgc tacaactgtt gctgatacta attctcctgg cgcatcaaac
     5281 gcccctgaca tagcagcaaa gtttacattt ccacctatat aagaacctgt catcatagct
     5341 gcaacaatat ttaaatctgg tactacattt tttaatgcta aaaatccaat catagctcca
     5401 agtacagttc ctacagaacc tacaagaaat ataatgagca atcttccact ttctttccat
     5461 atctttttaa tattacattg atataacaat aaagctatgg ctaaaggaac aacatatccc
     5521 catacttggt catatacaac agactctact ggtataatat ttagatttga taataacatt
     5581 gctcctacaa gagctattat tgctcccgac acttttgatg cccaattgta cttttgttcc
     5641 aagtaaatac taaatgctgc ccatccagtt acaactgccc ataataccca aatattatca
     5701 ggacttacaa aactcttcat cttaattacc tccatttcat taaattaaaa tatcattatc
     5761 aatattcaat atattgaata ttaatactat tataatgcat cttttcagaa aattcaatat
     5821 aacataaaaa atacatgcat tataaaactt ttttttattt tattaatctt tataaattat
     5881 atgttataat catgtctgag ggggtttgta tctaatgaat atagatgtcg gaagcaaaat
     5941 tagatctctt agaaaatcta aaaatataag tatctctact ttagcaaaaa attcagattt
     6001 aagtacaggc ttaataagtc aaattgagcg taatatggtt gttccatcta ttgtagcaat
     6061 gtggaaaata tcaaaagctc ttgatataaa tataggttat ttttttgaag agattggaag
     6121 agaggactct gatatagtag taaagaaaaa taatcgaaaa aaaatagtaa ctaatgattc
     6181 tacaaaattt tatgaacttc ttgttcctaa tttatctggg aaaaaaatag aatttatact
     6241 tgttacgcta gatggtcata cacaaaaaaa cagtgaattt gtgacacacg aaggagaaga
     6301 atgtggctat ataataaaag gtaaaatgaa gataacactt ggaaataagg aatatctatt
     6361 ggaggaagga gatagttttt attttaacag tactatacca catgtgtatg aaaattatga
     6421 tgatgaggta tgtatctcag tctgggcaat gactccacct tcattttaac atattgagtt
     6481 cttacttaaa tataaattga taaaaatctg aaataaatta gtatgataaa ctttactata
     6541 atttatttca gatttttcta gtttattatt tatatatcag tcattatata ttaagtaggc
     6601 agttctcgtc taaaatgtta gataatatct cttcagttcc actcattcct ttgaaagaac
     6661 ttgatataaa tgaattttct atttcatttt tatttatatc accatgcttc aatacatagg
     6721 atttatttga gatattaagc ataccatagt ccataataga atctccaact gcataaaaat
     6781 attctattcc taattcttta gtcaagtatt ttatagcatt ttcttttgaa atttcttttg
     6841 gtataaaata tatttttctg ccactcacat aaaacttcca attttttgat tccaatatat
     6901 ttgtatattc tttgattgaa tccaaattaa atcttgagaa atcaacaact atataaaaaa
     6961 atgtatcttc tgcaacttta aaattagtta ttccatctac attcaaatag tctttaaatt
     7021 tatgaatcat actttcaatg ggctcataat caggttttag tttatctatt ttttcactcc
     7081 atgatttcaa tatttcatta tctttaagta taactcctcc atttgaagtt atactccaag
     7141 gaaaataaat cccatattta ttaaattcta tacgcttaaa ttgctccact gttctagtag
     7201 ttgtaggtat aaacatacca taatattgta tttgctttat taaatttata gtatctaaag
     7261 atatataaga tatttctttt tccctataaa tttctatatt agcatattta ctatcagcat
     7321 ttaaaaattt attggagtaa atgatactcc tgtctaaatc tgaaaaaaca atcattcaat
     7381 attctcctgt ctaatatatt tcctaaatat ctttcatatt ttttattatt ccacaacatt
     7441 tataggcttt taaatccatt tgttcaactt caacattttt ctccttagct aatatgaata
     7501 tatggtctaa agatttatca tttaagtctt taacaagaat cttatacgga actcttctaa
     7561 gtaaaaccct tgtcgtttcc cctacacctg gtttgataaa gttaatatct gttatgttat
     7621 acttttcttt tatatttaaa acatcaaagt atccatctct tgttataatg tcactttccc
     7681 agttttcaac ttcatcagat atattcttaa attgattttt aaaacattct tctatagttt
     7741 cgatatagtt catagattca tctttatctt ttaaatgtct ataaaactta gctccatgaa
     7801 agtcattttt accaattaaa tcctctctta gtacagttct acttactaaa ccagaaacgg
     7861 ttgaatttaa acatgcactt ggtattaaga aatcttctct aattccataa agtcctgaat
     7921 atccagcagg gtcagctaat acagcaagtg ttgagtcaaa atgtttttta aatttaacat
     7981 taagttcatc acatgctttt tcaagttctt ttgatatagt tcccttacca gtccatccat
     8041 ctaaaaattg aatttttgag ctcggatgat tctttactat atattcaatt gcattcatat
     8101 ctattccctt atctcttatt atagagattg tatagtgagg taaacttaca ttatacttgc
     8161 ttcttatata tctttttgcc aaaataccaa taggtgttcc tgctctagca agtgaaacta
     8221 gtactacatc atttccattt tttttaatta ttttttcaca cataactcct atagaaaatg
     8281 caagcttatc tttattttct cttagtttct catgatataa attcagataa tcttcactaa
     8341 cttcatactc tattggtatc atttctgaat aatgtctacc attttgtatt tttaattctc
     8401 tttctttatt tccttcttct tcaattaaat ttgatatatc ttttaataaa aaaataacat
     8461 catctttaga atacgtgcta aaatctctca tatgactcaa ctcctatctt aaaatcgtga
     8521 tatattttat cctgttaaaa cactttagct acaacatact atagttaatt cttttatact
     8581 tgttttttta attatatcaa taaattctag ataagaattt tcacttttat tcagctctac
     8641 aaataaataa cacttagaaa aattatgttt atctatattg taaatataat tttgaatatc
     8701 tatattataa aaactcttta gcataaattt acttttaata ggataatgct tcttatcaat
     8761 attaataatt ggacttcttg ttgttgaatg atagtatata tcatattgat tttctttttc
     8821 ttttgcaaac agcataggta tatacataaa ttcttcagta cctaaaaata gaatcttttc
     8881 tttttcattt tttaaagatt ctttactggc catagttatt tttttacttt ctctcttaac
     8941 gatttctttt aatctaaatt gttctcttct attaattcca aatcttcctg tatattttac
     9001 atatttctta tttccaatat aatcttctaa atcaacataa atatgattta ctactaaatt
     9061 tactttatca cttgaaatag tctctaattt agtaatacta taattatcgt tgtaatttga
     9121 aataatatct tcatctatat aaaatttaaa gtttccttta aataagtata caaaatctat
     9181 cttacaattt agctctcttt ctaaacgagt aactttactt aaattttcat cacttaccca
     9241 attgagtatt gagcatatag tgtatttctt tttaggataa atgctttgaa ttttcctaat
     9301 tatattcatg catgtattgg ctgtggtaat ctcatcatca accaatatta tctcatctcc
     9361 aaatttaaag ttgttaagca taccaaagta taaattctgg tctgttgcat gggaatgttc
     9421 ctctaaaaat tccagatttt ctaactcgtc aaactgctct ctagtagtat gaataaattc
     9481 ataatctcca cataagtaat caaaaaaact atgcccaaca gctgtaccag tttcagcaaa
     9541 agctataaca gtaccatttg attcttcctt atttttttca ttataaattg tagccattaa
     9601 ttttcccaaa ttatccatct cacttggctt acatgctaaa tgtttaccga gtttctttga
     9661 gataaacaaa aaacgtcttt tgctattatt tcttactcct acgtctaaaa aatcatttag
     9721 ttctaaattc aatggattat caataatgtc aatctcacaa ttaaaattta agcaattctt
     9781 catagtctac tcctttattt aatacaccat atattttaga tagaatcaat gtttttttag
     9841 cccataaaaa atgtggattt gtttcattca ttctctctcc taatacaccc ttgctcacac
     9901 catacttact gtctgtacta tccagtatat ccaaagcatc agtaaaatct tcatatgata
     9961 ctgtagatag cgcttgtaca atttgaattt gcattggatg aattacggtc tttccaatta
    10021 atccatgata tttatccaat aatatttctt ctatatactt atttcttata tccttatttg
    10081 tcatatcaaa aaattctgat actccaccac ttataggtat atctaactct ggtctttgaa
    10141 gatatgtgat tatatctaat ataaatgaag aacaaatcaa attattatat atagagaagt
    10201 ttttatctct ccttatacca tacatcccta atatatcagt tatgcctact ctaatgctta
    10261 atattcttga cttatgtctt aaaatagcat tgtacaaatt agtaaaacta agttcttttt
    10321 tttcattata tataaattct cttgtctcta ttattggaat aacatacata tcttgaagat
    10381 tcatagaata taaagcttct atacaatttt caatcaactc cgaatttgct ttaggaatta
    10441 atattcctgt tatagaatga ctatttttaa ttataatctc tttaattctc aacagttgct
    10501 ccacatcttt tattcttaca aaaattagtg gtattccatc tttattagtt atactttcat
    10561 tgctaatatt ttttaaaatt aatctaagat tttctatagc ctctaactct ccatttactc
    10621 caacagcatc ttctaaacaa atagctaatg gtctaactcc cttaacatcc cctataatac
    10681 tcttataaat catattatat tgagtagctg gaacgtataa aaaagcgccc agtgcatatt
    10741 tcaacacatc tttttcagtc ttagcactaa agtcttctgg ctccttcaaa aaaattctct
    10801 ccaaatcatc tttgcagata taatcgaaat atttcaatgt ctagcctcct catatacata
    10861 gaaagggcca actttataca aattagccct taatataata ctacttatta taaattatag
    10921 tcgtatttaa ttcttccaat ctctttgtat tatccttaat taaagctcta ttgttttctt
    10981 ctattctcat agtttcatct ataccagact tgatagtatt gtacattttt tccaatgttt
    11041 ctatttgaac agaacttgtt ccagccattc tagctatttg tgcactttga gtacttacat
    11101 tttgtgcatt tctaagcaat aattcattag ttttgtcatc aagtgctttt agagattttg
    11161 cttgtaattc ttgcttttta agtaatatag cttgagataa acattgttta aatactggta
    11221 aagttacgac aaatgcacta tttatttttc taattaaacc ataattatta tgttgcatac
    11281 ctctaagcat aggtattgtt tggatagcta tattttctgc tattcttaaa tcatagactc
    11341 tttgatttaa catatcataa acttttaata gttcttgata attgacaaca tccatttggt
    11401 ctccactagt ctctgatttt tgtttaaaga aaggtagtat ttcattatcc atttcttcta
    11461 ttgccatttc tccagcaaca atatattttt gaagttcatt ataaaactca aagttttcat
    11521 ttaacatagc acctatttgc ttattagaaa gtgctatatc tctttcgtat tgctcaagct
    11581 caatttgaat tttctcaact tctcctccca atgtttcata cttttgaaac atcatttcaa
    11641 tggcattatt agcttttttg aaaagttttt gaacgaaatt tggttcctta gtttcttgaa
    11701 aatcgtctaa atcaaactta tccattattt tagttaaatg aaccagcatt ttagaatttt
    11761 cttcatttct atttaatttt attgtattta aaaggctatc agaaactctt gctactcctt
    11821 cagatgcttt cctaccaaac tgtaaaatcg tatctggatt ttctacttct ataagggatg
    11881 tcaatgcctc aacttcctta gactttctca gttcattttt tttattattt gtatattcca
    11941 ttatatcgaa gttttcttct tgaactgctg taggcaaatt ctcagtaacc tctacagagt
    12001 tcttatcttt tgaaaaacta tttatattta tccccatcta aaatcgtctc ctctttccaa
    12061 aaattttttt aagtaaaggt atatcatttt caagcgaatt taatttaagg tcatatacca
    12121 ttttatccaa tctgtgtata tcaatcaatt ggttatttat aacattctct atattgtttt
    12181 ctcctcctgg acataatatg ataatatcaa atccaatctt actaagtaaa agcataagaa
    12241 aaactatttc tttatcaaat acaatactct tgtacatgta tataatcaat tttgggttta
    12301 taagtgagta atcgaaattg ttcatcatat gaacaaattt cttatccatg tgaagtactg
    12361 taaatagacc ttttatcttg tcttctccac ttaaaccatt caaaaacata cttccttcta
    12421 acaactcttc cactttctct aaaatcataa tttgttgatt aagtgctagt gttgatatgg
    12481 aataatcttt attatttaaa acagagtttt tatctattac accttttgaa tccaataaaa
    12541 aggataaact aaaagcttct ctcgtaaaag cttttgaaaa tctatctaca tctccattaa
    12601 attcaataaa tgttgaactt tccgaatctc ttaataaatc taaaaactta atatattcat
    12661 tcttatcatt atatattcca ttaatcttag aaagaaaatt tggagcttct acaatagcat
    12721 cattaaactt aaatcctggt cttaatttaa gtggttgatt ccagtatatt gatatttcat
    12781 caacagttga acttagaagt agatttttta tttttctatc ttggagttgc caaggtttta
    12841 taaatcctgc atcatttaac aattcctcac ttatacgctt tgatgcttct gccccaacag
    12901 taaaagcttt ttttacagaa cttttatcta ttttttcccc taaaataacc ctttcttcaa
    12961 aagaaatttt ttcttctatt atgttggctt cttcaaactc tatattgtat ctttctgatt
    13021 ttaaaatatc tatattactc tttgaatttg gattaagata taaaacatca aaacctgcca
    13081 agtataatat taagagtaaa taaacttcat gtttttttat atctccataa aatattactt
    13141 ttggagcttc atttttattt atatctaaag caccgatata aatttttatc catgccatta
    13201 ttttcactat aaaattttgt ttaactctat cattagtaaa ttctttttcc tcttctttta
    13261 acactctatc cattgcttgc tttattttat aatcaatctc ttcaaatccc aaaatattta
    13321 tatctacatc ttccatgcta gttctatatt tttgaagtcc acttttaaaa taatttataa
    13381 tatcaaaatc actaggattt gctataacat cagtaaataa agcaatatct tttttgctat
    13441 caatattttg ttttgtctta aatatctcga gattgtacaa aacttcatct tctaaaccaa
    13501 tatacgaaat aaaataactt agatatccct ttgaaggagt gtaatttcct ctatctgatt
    13561 ggctggagaa tattttttca aatacctttg agtcacccat aactatcctc ccaatttaaa
    13621 tgtttattca gaactgaccc aaaattcata ttttgagtca gtctgttttc ttctaaaaaa
    13681 tttgttttaa gcagtctttt ttggttttac cagtcttatt atacatttta caacttcatt
    13741 aaccacaatt acacttgctg ctacacataa aacttttacc catgtcataa aatccattgc
    13801 tacagagtta aagaaatctt ggaatagctg agtaaatatt acttgtgcaa ttcctgtcaa
    13861 cactattatt tgtagtgcta atttgttatg tgtaaaatta gggaagatac tatctgcatt
    13921 aaactctcta cagttaaatg cattaaacag tgcgctaaaa gcaaataacg caaacattac
    13981 tgttccctta tgagcttcat caactcctaa gaaattaaat gtagattgta acattagtat
    14041 tgctgttata taaaatgcat ttaatactat tgtatacacc attgacttag aaattatatt
    14101 ggcttttcta tcaacaggtt ttctatttaa cacatggtct ctaactggct ctaaacctaa
    14161 tgctagtgct ggtggtccat ccataattat gtttacccat aataattgta ttgtagtaaa
    14221 tggcatctct tgtcccataa cttgagatat tatagccact aaaaatgcaa ttacatttac
    14281 agttaattgg aattgaacga atctttggaa gttatcataa attcctctac cccatttaat
    14341 accttttaca atggtactaa aactatcatc agttaaaatt atgtctgctg cgtttttaga
    14401 tacctctgta ccagatatac ccattgctat accaacatct gcttttgaaa gtgctggagc
    14461 atcatttata ccatcacctg ttactgcaac aacatccccg ctcttttgca gagctgaaac
    14521 tattctcatt ttactatctg gtttacttct tgccacgatt gaaatacttt gtatctcttg
    14581 ttttaattct tcatcatcta aagtatctat atatgaagct tcaaccactc tcatattatt
    14641 ttttaatagg cctaactctt cacctatagc tcttgccgta tttatattat ctcctgtaag
    14701 catttttaca gaaacaccag ctttatttgc tgtatttaca gactctataa catcttttct
    14761 aagtgggtct cttataccaa caaaaccact aaatactaaa ttattttcca ttgcacttac
    14821 attcatagca tcactttcag cagttactgc tgcttcttct tcagcttgtg agatttcttt
    14881 atatgcaaac cctaaagttc tcattgattt tatttgcaat ttctttattt cttctaaaat
    14941 ctcatgtttt atattttcag ttaatggaac tatgtctttt cctctttgaa tatgtgaaca
    15001 taattcaagt aagacttctg gtgctccttt tgataatagt acatgatgat tatcttgacc
    15061 tataacagaa gtcattctct ttgaatctga agtaaaagga ttttgagcta ctatattaga
    15121 attttttcta acttgtctat aatctacgtt tttgtaatat aaaagcaatg cacattcagt
    15181 agcacttcct aaatacttaa tatcaccatc attgtgctct acatcagctg tagagtttat
    15241 catacagttt tcttcaaaat aactatttat ttcctcttct gaactttcaa tatatctgcc
    15301 atcaacatat gcaacttcta cagtcattct attttcagtt aatgtaccag ttttatctga
    15361 acatattaca gaaacagaac ctatcgtttc acaagcttct tttttggtaa ccaatgcatt
    15421 tattttagcc attttttgca ttgttattgc aagtgtcata tttatcatag ttggaagacc
    15481 ttctggcact gctgcaacta tcaacgcaac acatactgta aatgctgttt taactgggtc
    15541 tattgattct aaaaacggta atattccaga catatctaca tttaatacat tcttacttat
    15601 catttggaaa accataaata taaatagtaa tcctgctact gcactagaaa ctttggctat
    15661 caaaccacct aaatttccaa gttttatctg aagtggagtt tgctcatctg tttctgataa
    15721 gttttgagca attttaccca tttcactatt atctccaatt gaagttacaa ctagagctcc
    15781 tctaccataa gctacaagtg taccaccaaa caccatattt atctgttttg caggtattgg
    15841 ctcctgtact actactgctc ctttagattc tatctcttcc atagaaacta ctacatcaca
    15901 ctttttagat acgtcatcag attctcctgt taacatatct tctctaattt taaggtttat
    15961 actttctata agtctaccat cagcaggtac catgtcacca gtttcaatat atactatatc
    16021 acctggtacc aattcactct tagaaatttg atgtacttcc ccatctctca atacttttac
    16081 ttctatgttt tcagtcaact tagaaagagc ctctgctgct ttcttagaac gaccttctgt
    16141 aatcatacct attgctatac ctattgctat agcacctaca atccctattg catcatgagt
    16201 ttccccaatt actgcactta ctactgctgc aataatcaag ataactatca ttggttcact
    16261 taaactttct tttaaatcat cccaaaaact accctcttct tttggagtaa attcattcaa
    16321 accatatttt tctcttctag tcttgacttc tgattctgat aaaccttttt caggattaac
    16381 agataagtct tttgtaactt cttctttatc tacattataa tatttgatat cattattaaa
    16441 ttccatatgc acactcaatc ctgtctaaat tttgcattac ttctttaaga cccgcatctt
    16501 ttgttccttc accaattgct ttaaattgcc aacttccatc ttttctatat atagctccta
    16561 ctataagagt agttttttca gaataattat cagatagatt gtattttgct atctctttct
    16621 tattaccttg gtcttcaact cttatatagg cattttctat cattccaaag tgttgttttc
    16681 tatctacaca attgtatata tttactacaa acaataattt atgaatattt gatggaactt
    16741 tatccaaatt aactaggatt tgttcatcat cacctgtacc ctcaccagtt agattgtctc
    16801 ctgtatgcaa aactgattta caggcacttt ttagatttcc aaaataaatt aaatctttta
    16861 ttccacttaa tttaccttct tgattaagca taaatacaga tgcatcacaa tctatatcag
    16921 cttgtcctcc accaaataac gatttaaaaa aacctcctcc agattgttgc acagggtccc
    16981 atcctagacc tacaagtatg ttactcagtc ctgggttaga tttttttaaa tctattttat
    17041 caccttttga taaattaatc gacatactaa acctccacaa aattttatat aatataacct
    17101 atacattatt tgtataggtt atttatattt aatatattat attgcttaaa ttaaacattt
    17161 aagccatagt ttctacaaag tgcttctaag ccaccttcaa atcctgctcc aactgcacta
    17221 aacttccatt ctccattata cttatatatc tcacatactg taattgcagt ttctatacta
    17281 aattcttcac ctaagtcata tcttaatatt tcttcatcat tttcagaatt taatactctt
    17341 atatatgagt tgcttacttg tccaaaattt tgtcttcttt cagcagcctc atatattgta
    17401 actgttacag ctatcttttg tatatgcttt ggcattgtct ctaaattcac taatatttcc
    17461 tcatcatctc catcaccttc accagttcta ttatcaccta agtgttcaac tgctccagat
    17521 ggatgtctta agttattgta gaatataaaa tcttcatctt tttcaactct ttgagattct
    17581 ccaaccatga aaacacttac atctaaatca taatcatagc cactatcaaa tgaatttgta
    17641 tcccatccta gtccaagttt tatattttta agacctggat ttcccttagt taaatcgatt
    17701 ttttgtcctt tttttaatac tatagccatg gaaaatatcc ccctttagtt tattgttata
    17761 gttgaaatta taagtttatt ccaaagtttc cacataatgc agcaagacca ccttcaaaac
    17821 cagaacctag tgcattaaac ttccattctc cattgtgctt atatatttca gctactacta
    17881 tagctgtttc tatactaaaa tcttctccta attcatactt tattaactct tcatttgtgt
    17941 ctttatctac tattcttata taagaattac ttacttgtcc aaaattttgt cttctttctg
    18001 tcgcatcatg aattgtaaca gtaaaagaaa tcttgcttat ttctccagga atcttagata
    18061 aatcaacaac tatttgttca tcatctccat caccttcgcc agttctgtta tctcctagat
    18121 gctcaactgc tccagatgta tgttttaaat tgttgtaaaa tacaaaatct ccatcattag
    18181 taacacttcc acttgctcct gttaaaaatg ctgctgtatc taaatcaaag tcaaatccac
    18241 catcatactt gttagtatcc catcctaaac ctactaaaat atttttaaga cctggatttc
    18301 ctttagttaa atcaaccttt tggccttttg ataatgttat acccataatt tatcgctccc
    18361 ttgttaattt tagctaaaca aatcttgcta attatttatc tttttcttta atcatattat
    18421 aacatttttt attcatttgt gaagaaaact tgcttgatat ttttaaaata taattaaaaa
    18481 aatgtttata tttcttactt tttacataga ttttacatac tttattaaat gtataaaagc
    18541 tatgtttaat atgtaattat atatcattta tataaacaag tacatatttt attttgaaac
    18601 tacaaaatta ttgtactata ttgttttaag ttgttttata atagattata tcatcttatt
    18661 ataggagaaa aaatatgatt tgtactatta aaccattaaa gatttcaata aaaaagtcaa
    18721 ctgaacctct aattgaattt atatctgata ctacctataa aaataagagt tcttcatata
    18781 ttaaagaagt ccactttaca agatatatag gtgttgatga ttcaattgat aaatacatag
    18841 aaaaagttaa aaatatagat acttattttt ttaataatcc tacaattccc tatattagat
    18901 taaacaatct aaaaataaat tttgacaaag aacaaacaga taagatgctt aaaattttta
    18961 atatttatga tagttctaag tttaacagtt gggatttata taatatcgac tttccatgta
    19021 aaatagaaaa tgacacttta aattggacta aaaagattgc atttaaaaat actttagatt
    19081 tattttccac aagtatgcca aattgtacat cctctatcat caaaaacttt gcagttaaac
    19141 ttttgtgttg gatggactat tttctaccta aattatttta taacaaggtg aagactacta
    19201 tttcaccaaa gatagtatac tttggaaata ttaaaaggca agaacttttt ttcttatatt
    19261 ttttatcaca acttgggtgt gatattttat atataaatcc aaataaagat attcttgatt
    19321 tatatcctga atgttcaaaa ttttctactc tatttgaact ttctaggaaa actccaaata
    19381 tattagaaat accttttgaa aggcatatta caaatataaa ttacagtgaa aataccaata
    19441 agacttctcc tactacgaat aaacctaact ctaaaaatac acttaataac acaaatctaa
    19501 catctaaaaa aattgcacaa gatgaggaag tagaactttc ttatgaagac cttgcaaagt
    19561 tatcagcttc tgttgttatg attgttgtgt gcaataacga aaataaaccg tttaagagtg
    19621 gttctggagt agtaataaat aatgaaggtt atatacttac gaatcttcat gtagtcaatg
    19681 atgggtattc atttttagtt agatttgaaa atgatgataa agtttataca tcttaccaaa
    19741 ttataaaata ccattcagat tatgatttag ctgtgataaa ggttgataga aaatgcaaac
    19801 caatacctgt taaagtcagt aaaaaacctg tgagaggtca aaaaatagtt gcaattggaa
    19861 gccctttagg tctttttaat acagtttcag atggaattat ctcagctttt cgtgattttg
    19921 aaacggttca aatgatacaa tttacagctc caatctctag tggtagttca ggaggagctc
    19981 ttttagatat gtttggaaac ttactaggat tgatttccgc tggatatgat gatggacaaa
    20041 acttaaattt agctgttgaa agtaatcttg tcaaaatatt tgccaataat tttattgaga
    20101 tagtaaacta atcaatcaaa attctactta tcctattcca taataatcaa gagggtgaaa
    20161 acattgcatg cttttgccct ctgattaata ttgaaaaaag tattaatact cgacattttt
    20221 atgctcaata aataattgat gccatagaca aaatgtaaat atagtccata tttttctcga
    20281 attatctcta tgacctttaa gatgcgtatc taaaagtttt attgcatatt ttttatctat
    20341 aaattcatct acatttgaat tataaatagt ttcctttaca atatccccta gagaatcttt
    20401 taaccaaact cttattggag ttggaaatcc taattttttc ctttgaacaa catattctgg
    20461 gactatttct ctaaacgcct ctctaagtaa aacttttgta ttattcttat ttattttttg
    20521 ctccattctt aaattttttg ctacatcaaa tacatcttta tataaaaatg gtactctaag
    20581 ttcaacctgc tcagctgtag aaagtttgct aattttttga agtatatcgc cttgaagcca
    20641 tgtattcaca tctatatgtt gcattttact tatataatcg taattatttt tttgtgcatc
    20701 tctatacaaa ttagacaaca ggtattcata tatattcttt tccttatact taaaaaagaa
    20761 tctttttact tcgctgtttt caaaaacttt tgcatttcct atatatcttt tttctaatgg
    20821 tgttgtagct ctataaagat aatttcttcc ttttatgttt ggcataagtt ctgaaactct
    20881 tcctaatata cttttaatat aagtaggtga attgacaact gatttcatag tactatactc
    20941 tttatatata ttatatcctc caaataattc atctgctcct tctccagaca atgctacttt
    21001 aacatgttta cctgcctctt taattaagaa ataaagacct acttctgaag ggtctgctat
    21061 agggtcatcc aaagaataaa ttacattcgg taatgatttt atatattcat cttgtgttat
    21121 attaatctgg atattttcta tacctaactt atcagctgtt ttctttgcta cttctaattc
    21181 actatatcct ttgacactaa agcctgcaga aaaagatttt atatttggat taatttgtga
    21241 agccacagta gttataatag atgaatctat tccaccagac aaaaatgttc caatttcttt
    21301 ctcctcttcc atatgagtta ctattgaatc tactactata tttcttatat catcctcatt
    21361 cacatctcta ttgcctaagt gatttttagt ataaaaaaat tttgtactac ttctaaactc
    21421 aagtttatta tatctcttta aagatagtat accattttct accctaaaat agtgacctgc
    21481 tggtatcagc cttattcctt gtatcatagt atcttcagga agtacatatt gaaatgaaaa
    21541 ataactttgt aagcttcttt catttatatt taaatttttc atatattcaa gtaaaaactt
    21601 atactctgag gaaaaaatta ttgcattttc cttatttata taataaagtg gttttatccc
    21661 aaataaatct cttgctccaa atatactttc attttctcta tcatagatta taaagttgaa
    21721 cattccttta atcttatgta cacaattctt accaaattct atataagatg ccaataagac
    21781 ttcaatatca tcatttgtag taaaattgtg tcctttttta attaattctt cttttatttc
    21841 atgatagtta tatatatttc cattaaaaac aataactttt ccatcttttt caaatggttt
    21901 atttccataa atagcatcaa ttacattaaa tctttcaaaa tcaaaagttt tttcctcatc
    21961 cttatactct attctactag aaagtcttgt attatttaca gattctttta ttttaaattt
    22021 aatatcttct tttttagata tatcaccttt ataataaatt gaaatattgc cgtccatata
    22081 atcaaaattc ctttctgtcc tttagtctta ttctatattt catagttttg tctttggctt
    22141 taaaacttat tcaatttaaa atgaaattgc tattaaattc ttataaatta tctgttaata
    22201 tttcatattt tttttatttc tagaaatact atattaggag gtgattttta tgaagaaaaa
    22261 aatattaata ccagttatta tgtctttatt tataatctca cagtgcataa cttcatttgc
    22321 ttttacacct gaaaataaca aatttaaggt taaaccatta ccttatgcat atgatgcact
    22381 tgaaccttat atagataaag aaacaatgaa actgcatcat gataagcatt atcaagctta
    22441 tgttgataaa ttaaatgctg ctcttgaaaa atatcctgag ctttataatt attctttatg
    22501 tgaattattg caaaatttag attctttacc taaagatatt gctacaactg taagaaataa
    22561 tgcaggtgga gcttataatc ataaattctt ttttgatata atgacgccag aaaaaaccat
    22621 accttctgaa tctttaaaag aagctattga tagagacttt ggttcttttg aaaaatttaa
    22681 gcaagagttc caaaaatctg ctttagatgt ctttggttct ggttgggctt ggcttgtagc
    22741 tactaaagat gggaaattat ctattatgac tactccaaat caggatagcc ctgtaagtaa
    22801 aaacctaact cctataatag gacttgatgt ttgggagcat gcttactatt taaaatatca
    22861 aaatagaaga aatgaataca ttgacaactg gtttaatgta gtaaattgga atggtgcttt
    22921 agaaaattac aaaaatttaa aatctcaaga ttaattaaat attaaaatcc ttttgaaaaa
    22981 aacttatgtt tgctatataa tataataaaa gatagtctaa atttaccaat ataatgttta
    23041 tagtgacgat tcaggaggat ttttatatga taaaagaaac acatgaaagg ggaggttata
    23101 tattcgcact tttgatactc ccctttataa acaatctgta cctagttaaa tacgatattg
    23161 catataaaat agtattaatt ataatttata tttattttgc atatttaggt tctttatttc
    23221 cagatataga tatgagaggt tcttacataa gtaaaaaatt tatgttaata tacaaattat
    23281 ttggaagtag gtttagacat agagggttta ctcacagtct aattgcctta ttacttattt
    23341 atagtttttt taaatctctg acaatcttca ctaacaataa tatagttttc tcatgtttgt
    23401 ccagtgggtt tataatagga tatctatctc acttgtgtct tgaccttatt actaaagaag
    23461 gtattgaatt gttttatccc attaccataa acatttcttt actacctata aaaactaact
    23521 caaaaacaga aaaattcata tctaagctat taaatttcat agttattttt ttgattggat
    23581 atcaatttta tattttatta tagttgtaga aaaaataaat aacaatattt tgaactaagt
    23641 ataaataagt tttatatatt ttagtttaca aaagaactaa aaattttgta ccttaataca
    23701 aaaaataaaa cgtatcttaa aataatttag tactaaatca ttttaagata cgttttattt
    23761 cgtacaagct aaatcatttt atcatcttta tcattgtgat gttttttatg atggaaatca
    23821 taattatgat tttcaagatt tttatttact ctatttccaa tcttccatga caaaaacacc
    23881 aataatataa atgatgctat ctttaaagga cttgttataa aaccttctat atcacttgcc
    23941 acgtaactta taactataaa cattacaaat tttcctgcca acattgctgg cacaaaatct
    24001 tttaaatctt ttttaccaaa agctgatgcg atagtaacta aacaaccagg cataaatgga
    24061 caggcatacg ctataaataa taacttaaat ccctgtttat ccatccaact tattgctttt
    24121 tcagttttac tatttctcaa cttattaaaa agcttactat cattaaatct gcttattaat
    24181 aagaataatg aagttgtccc aagtcctgag ccaatccatg ataacaaaca tccaataaat
    24241 aatccttgta ttgcagcatt tgctgtaact atagcaacaa gtggtaaggc tggtataaag
    24301 ctttctataa atgcacttaa taagccgatt attgtggcta acacccaata attttgagtc
    24361 aaatcaagta attcttgtat atactgcata ttgcttttcc ccttttttaa ctttaactat
    24421 tattatatac tctatatgtt tattatatac caatctcaaa aaatgaatct aatattaaaa
    24481 agtaaataat ctatataagt ttacagttaa ggtataatta ttttattata tattataaca
    24541 aaaaaggtga tgtttcaaaa taaaaatctg aaacatcacc tttttataac ttaaattgtc
    24601 atgtaagtta taaaaaatat atattagaca taatattact aaaaatgtct agtcagagta
    24661 aaataaatta cacagtacaa caaatattac tatttaattg agttacttct ttataacatt
    24721 taaccttctt cctactctgc tcaataattc attcgatata ctacatgagt tatttgcaac
    24781 ttcagaagca gataattcag ataaattatc tcttcctatt attattgccg tattaccaac
    24841 ttctacatta ggtatatctg taatatcaac agcaagttgg tccatgcaaa ttcgacccac
    24901 tattgctgct ctacaaccat ttattattac gtaacttttt ccacaggata aatttctagg
    24961 tataccatcg gcatagccaa tagataatat agcaattacg ctatctctgc ttgcaataaa
    25021 gactcttcca tatccaaaac tatcatcttt ttgtattttt ctaataagaa ttacttgtga
    25081 ttttaaggat aaaacaggtc gtaaatcaag ttgcaattta gtcgtatcat ttggagaact
    25141 taatatcccg tataatgcaa ttccaattct tacataatta cacttaagat tgggataatt
    25201 aaaaaaacca taactacttt gaatatgtac tttaggaatt tttatacctt tttttaacag
    25261 aaaatcaatt aacttataaa aacattcaat ttgtttattt gtaaagtcta catcttcatc
    25321 tttcagacta tctgaaacac acaagtgtgt aaatatgcca catatatcta agtattttaa
    25381 tttaaatgcc tttaaaattg acctcacatc attaacatca aaacccagtc tgtgcatacc
    25441 tgtatcaatt ttaatgtgtg attttatgtt gtatttctgt ttgtttaaag agatagcatg
    25501 attaaggtca ataacactct gtgtaagatt gtatttatga agttcctttg cccttattgg
    25561 gttagtatat ccaagaatca atatttctcc tagtattcca tatcgacgta aatcaatccc
    25621 ctcatctatt gttgctactg caaatgcttt tacacctatt tggtttgcac aagtcgctac
    25681 ttcaaaagca ctatgaccat aggcattagc ttttacaact gccatcaatt cacaatcttc
    25741 tggcatagct ttttgtatca tctttacatt atgttccaaa ttatttaaat caatttcaat
    25801 ccaagctcta ttggtattta ccttatgttc tcctttcaag tcttttttta gaggtttaag
    25861 aatcaatata ataattatag aagctataaa agatgctata cttaccacta tataatgaat
    25921 aaggctattg tcaaccaaga cagtttgtat tccgaatatt ttagaaaaca tacgaataac
    25981 aacaatcatc ataggatgta taatataaat aattaatgat gatgttcgca ataacctagc
    26041 tcgagaacct tttaaaaatg ttaaggctgt aaataagaaa tacatacatg gcaataacat
    26101 aatatacatg ctatcatgac gctgtacccc tagtttatgt aaaaacattc cctcacataa
    26161 cataagtaag aatgatataa gaaatccaat caagctattt ttagttgaca tataattaga
    26221 tttattagat attattccac caagtatgaa aaatacaggt gcaaaaaata taccatttcg
    26281 tgtataatct gatacttcaa aaatataccc atataaattc tttaatagtg gtattttttc
    26341 taaaaaacca taataactat caccaaacat accaattaca taaataacaa tagtgattac
    26401 taatgagtac tttaaaccta gtttttttac aagaaagtat gcaatacttg caccaataat
    26461 tgatgcaggt agataccata aatgatataa agtcccatca aatacaatgt ccttaataat
    26521 attgggcact aaattattca tggaaaaata tccagtgtaa atatttaagg gaatataaat
    26581 tacaatagct atcccgtaaa taaataatac attttttaga aaagatttca gtttattaca
    26641 attataatta tatgttgaaa ttagaaaaaa gcctgatagc ataaaaaaga atggcacagc
    26701 aatacgagca attatactag ttaacacaaa gtcagccatt ttactatatg ataataatgg
    26761 agatgtatga attgcaatta taagtaatgc agctataatc ctaaaataat caagccctgc
    26821 ataagattta ttcctaatca ttcttgttcc tccatttctt agtcctttct atttctccaa
    26881 aatgtcacaa taaaaccatc cttattgtta cccgatattt gatatacaca ttgttcaggt
    26941 atagagattc tcacttcttc tttagtatga ataggcacat aaaagatttc tataattttt
    27001 tctttatctt ctatttttaa acaatttgta tacgaaaatc tcttaatata ttctatatat
    27061 tcttcaaggg cgagattcat tttattgata atctcagaat gtggatatcc gacataacga
    27121 aaatgccatg gttcttgaga tattcctgtt atttcttctt ttttacactg ataacgctct
    27181 ataaaaccat aatgtagtgc tgccttacgg aactcattac aaataccatt ataaggaaat
    27241 tcagggcaga taaaatctat aacctctttc ttcaacccta aatcaatagc aagccctgtc
    27301 tgatgttcac tgtgatttgg aagtgctaca aactttttag taaagtcttt tccattgtca
    27361 cgcagagagc ttgaatatat atcttgttgt tcctctgcag tacgatatcc acttacaggt
    27421 actatttcat ttattccaca tatacttttt aaaatatttt gtaaaatatt tgtagcttca
    27481 cgtttcataa gaatacttgg aaaccttgta tctacaggaa ttaaatctat atcttcagat
    27541 gttttaactg gtaaaaaagc atttactaga attagattcc catcataaac tttatcttta
    27601 gtcaataaga tagtatttgt cattttatct tctttatttt tatctaataa aatattattc
    27661 atcattttat gtacacctca attatcttat ccaatatttc ttcaaaagac agtccaatac
    27721 ctttcatcat attcggataa cgactatgag aagtaaagcc aggaattgta ttgacttcat
    27781 taaaaataat ttcaccagat ggagttaaaa acatatctac tctagcaaat ccagaacatc
    27841 caagagttct ataaataatt tttgctgtct cttgtattct tttctcacaa tccaaatcaa
    27901 ttctagctgg catatgaatt tttgaacttt tcaatgtata tttttctgta taatcaaaaa
    27961 atccttttga caattctatt tcatctactc ttccaacaag taagtcatcg tttcctaaaa
    28021 ctgcacaccc tacctcaaaa ccatcaatat tttcctcaat tattacttca ttatcatgtt
    28081 caaaagccaa gtctactgct acttgtaact cttctttttt ataaatttta ctaataccaa
    28141 atgatgaacc tgcacgcaca ggttttataa ataatggata agttaagtct tttgacagtt
    28201 cagccacatt aaatttatct gagtatttta gtaatattga ttttggtact ttaatacctt
    28261 ctgaatgaac aagtttatgt gatttgtctt tgtccataca taatgctgaa gaaagagtac
    28321 cacatccaat aacctgtata cctgccaatt caaataaccc ctgcactgtt ccatcttctc
    28381 cattttttcc atgcaataca ggaaatacaa aatcaagttt agtgtattta atatcatttt
    28441 cacatgattc aatagcacta tttggaatat gattctgaga aacagcatca ggaattaaaa
    28501 attcaataat accatttttg acacggttct gcgaaacaat aactggaatt atatcagtat
    28561 ccctttcaaa ccaagtgtta ttctggatat tattataatc tccaatataa tgataccaat
    28621 taccctccct tgtaattcca attggtataa tatcatattt ttccgtattc aaattctcaa
    28681 gtactgcaaa agctgattgt aatgatacat catattctgt tgagtttcca ccaaatataa
    28741 ctgcaatttt tttattattc atctttgatt ctcctttgtt gatttattta tttttataca
    28801 atctacagaa acatgttaaa atactttttc tatttcaaat ccaatcaacc ttcacttaca
    28861 attgcatctg ttccacataa tatattttca catttttcta ttgatataat tactgtatca
    28921 aaataaaagc actcttgttt tgatacagta attatatcaa taacaagagt gctctttatt
    28981 tatcctaaag aatttcctat ttttttatac ttaaattctt acaaatttcc tacaatcgga
    29041 ataataacct taaaggtaat tatattgttc tcgctatgag ctgttattgt cccgttatga
    29101 agctccacta tttctttagc tatagcaagt cccaacccag ctctaccact gtttgaatta
    29161 cgagatgtat ccaacctgta aaattgttca aaaatacgct caagtttttc ctctggaatc
    29221 gtattgctat gatttataaa tttaagcttt atataatcat cctcctgtac agctgttatt
    29281 tcaatagtag tatcttcaaa actataataa acagcatttc gtaataagtt atcaaataca
    29341 cgttgcattt tattaacatc acaccttatc atcatatttg gtgtaacatt cagtgtatat
    29401 tttaaattct tactcatcaa agtaggttta aactcatatg ttagttgttc aagcatacga
    29461 gttaagctta ctgtactgta ttcaagtgat atatttgata aattaaacct tgtaatttca
    29521 aagaattcat taattaaatc ttcaagacgc tctgccttat ccaacgaaat agataaatat
    29581 ttttttcgca gttcttcaga aatctgactt tcatctcgaa gtagtgtcag ataccctata
    29641 actgatgtaa gtggtgtttt taaatcatgt gcaagataaa caataagttc attcttacgt
    29701 tgttctgcca tttgtgtctc aaatttacgt tgttcaagta tatgtttaat tgagtttatt
    29761 ttcttttcta ttgccaataa ctctggagat aaagccactt ctccaacatc ttctttgata
    29821 agagagtcaa taccttgatt tatttcacta aaatattttg taaaccaatt taaataaaca
    29881 cggaatatta taaaaaacac aatcaccata gctagtatca tgaagagctc catgctatta
    29941 cggaaaatct gctgatataa attcaatgca gctttatatt ccatacgaaa agcattttca
    30001 aagaatgata ctatccagat agaaaactta ccatacaata taaaagaata taaaatccat
    30061 atgaaaataa tggctataaa tgccattaaa gctgttctca taaaaatttt atttctcact
    30121 tgagtaaagt caggattgac tttaatattt ttacttttca attgtataac ctactcccca
    30181 tacagttttt ataaatttcg ggtttttaga aggttcatga attttttcac gtaatcgccc
    30241 tatatgagcc ataactgtgt tattattatc aagatatttt tctccccata cagcttcaaa
    30301 caattcttca gaaggaacca cttttccttg atgttcacat aaataccaaa gaatagaaaa
    30361 ttcaatcggt gttaatgcta cttctttccc aaataaacta catttatgtg tttctttatt
    30421 gataatcaat cctttaatat catactcatt aacaattatc gactgttgtt cataagaatt
    30481 attatatcgc atatatcttc ttaattgtgt ctttactctt gcaacaactt ccaaagggtt
    30541 aaatggcttt gttatataat catcagcacc tatggttaaa cccattatct tgtctatatc
    30601 ttcaatttta gctgttaaca taatgatagg ataatagtat ttttctcgaa ttttctggca
    30661 aatatgaaat ccatcaatat caggaagcat aatatctaaa atagccatat ccaaatcttt
    30721 agattcaata cattctagag cttccttacc tgtataaaac ttgtatactg taaaaccttc
    30781 attttttaaa tatacttcaa ttaaatctgc tatttctttt ttatcatcca caatcaaaat
    30841 attttcgttc atagattatt tttcactctc cttattatcg aggtcatcat ttataatatt
    30901 ggtaaattta catatcaaat aattacaagc atatttgtaa ctaattttta taagaaaaga
    30961 actactacat cagaatgtgt tgatgctact attggaacac aaaaactgag attaattgtt
    31021 attataaaaa ctcccctaac acgtccttat tatcgcataa caggtattaa ctttcaataa
    31081 aaatatctat cagtcaaaca caattcaatt tattatacca gagacaatcc atataaaaaa
    31141 caattaaacc tatgtatgta accacagaca ttactaaaat ttccatgtta ttttatataa
    31201 aaattcaaaa aaggagttga atttaaaatt tgattagaaa aaaatcaatt ttgagacaac
    31261 tcctttttat tttaatgtat ttaaattata gtatatcagc cttcataata tcctcaaaat
    31321 cacctttttc agatattaat cttatcaatt taacagtaca gcttaaatca tttataagta
    31381 tagctccaac taatgaacca tctttcataa ataatttttt atatattctt tgaccttcat
    31441 cttttgatga aatttcttta tcaaaatttt gtatattacc aatactaaat acttttacat
    31501 tcattgcatc taatgaaact ggatatactt cactttcaaa tgtcaaatta tctccacttg
    31561 catttgctcc tgctattttt cccatttcta ctgaagatgg ccatattgct aaactaatat
    31621 ttcccacttg tgcaacatct ccacaagcat atatatcttt aacatttgtc tgtaatgtct
    31681 tatctacaac aatacctcta tcaattttta cagatgtatc ctgaaccatt tgagtgtttg
    31741 ctcttacacc aattgaaaat actaccatat cacagtcaac actatctcca tctttaaata
    31801 caactttttg agcttttcca tcaccttcaa taccatcaac tgctacaccc aatcttacat
    31861 ctattcctgt atctcttaca cattgttcta atattttact accctcttta tcaagttgtt
    31921 ttgatagtat gctatccatg gcttcaacta ctactacttc taatcctttt agtctaaatt
    31981 cccatgcagc ttcaagtccc aataaaccgc caccaatcac tacaactttc ttagattttt
    32041 ctagtgcttc ttttacacta tataaatctt ttatatttct aagagtaaac acattttcta
    32101 aatcatggcc cttaatcggt ggtataaagt ttctacttcc tgtagctatt acaagcttat
    32161 caaatttaat agctccatca ttcactataa tagttttatt cacttcatct aatttatcta
    32221 cattcgttcc taatattact actatattat ttttatcata ccaatcttta tcttccatgt
    32281 agaaatctga accaagttct tcatttatac catcagaaag tgcaggtcta tagtaagtca
    32341 actcatcttc attagaaatc atagttatct tacaagtttt atttcttttt cttatagcat
    32401 ctgctgcata aaaaccagct gctccatttc ctactattac gaaatattca tcagtatctt
    32461 ttctaaaagt aacttcatct tcttcaactt ctataaattg gtcttcctta gctccacata
    32521 cagggcatac actaggagca ctatcacctt caaaaacttc attacaaatt acacatttaa
    32581 atttcttagt tttcttttta cttccaaaag tagaatttaa cttatctagg aatttacctg
    32641 tataagaatg taatttatca atctcttcat ttgatggttt aaagttaaca gcaaaaggtt
    32701 ttactgctgt cattctaagc tgacttattc tctccatagc attttctata ccttctccac
    32761 tccatccata tgaaccaaat actgaagcaa cttttccacc atgaagaact gggtttaacg
    32821 atattaatat atcccatata ggctttaacg catctcctaa tattgttggt gttccaaata
    32881 atacaccttg tgatactgct atttttgcca aaatatcttc ttgcttatgc tctattacat
    32941 caaataaatt tactttatag ttgctatttt tttcaatata tgcttttata gcttctgcca
    33001 taatctttgt atatccatgt gcagaaacat aacaaattgt aacttctttt tctaacttta
    33061 tttgttcctt tacactccag ttataataaa ggtctataat ttttctagga ttttcagtta
    33121 atactggacc atgacctgga catacagtat ctatatctaa atcttttatc ttttctatag
    33181 cagttaccat agatggttta aatggaccca ttatacaatc ataatagtat cttaaagcat
    33241 ccaagtaatc cttttcttca ttttcatcaa gtgtatttac tatcttatca ttactatagt
    33301 gactaccaaa tgaatcacat gttacaagag ttttatcctc ttttatatat gtgtatatag
    33361 tatctggcca atgaagcata ggtacagaga aaaattctaa agttttgtcc ccaattgata
    33421 atgtatctcc atctgtaact gcaacatatt caaagtcttt gtttacgatt tccttaagat
    33481 atttaattgc tgtttctgaa gctactactt ttgcattttt agctaaatcc aaaagctttt
    33541 caacgcttcc tgcatggtct ggttcagtat gacttacaac aatatagtct attttttcaa
    33601 aatctatatt taaatcattt aatctttcaa tataattgtc aaaatgctta tctttaactg
    33661 tctcaaacaa aactgttttt tctgttcctt ttaatacata tgaattataa gtagttccat
    33721 aaggtgtata cataattatg tcaaatactc ttaaatcagg gtcaagtgaa cctacccaat
    33781 ataaattttc ttttattttt agtgatttca atgacatacc gtactcctcc taaaatagtt
    33841 atttagtctc tttataccct gcttgttata ttttaaacaa aatttattca aaaaagaaat
    33901 tattttaaag atagtttaaa tcttctaatc ttctttttct accttcaata cagttccctt
    33961 tttagaatct acttttatgt catattcatt tttatcttta tctattctta ttttataaaa
    34021 agtaacatct aaatctttgt ctaaactcca ttctttaacc attccatttt gttcttttaa
    34081 tgcaatttcc atagcttgtt gaggtgtaat tatgtcattt aaatctaatt tttcttttct
    34141 agcaactcca tttaaatctt ctgaatctaa cttttctgtt ttatcttcta atacatcttt
    34201 tgtattagca tcaattttca tcttatattc attattatca tctacacctt ccacagtata
    34261 aacaaattta tttaaatctc tctcaagact taaatctact atatcagcat ccttgtattt
    34321 atctttaaat atgtttattg catctgtgta tggcacttta aaatcttgtt ccattaaatt
    34381 ttcattattt ttttcatctt ttaaagcatt atttgaagca tttgcctcct tttttgtatt
    34441 atcttgtgct tgtcctgaat ccttagttgg gctcccacta catgcagtta ataaacctgc
    34501 tataagtata cttaccacta ataaatacgt ttttttacac ataaaaatcc tccttaaata
    34561 tattaattta ttctatatta tgccctctaa accctttatt taccctttta tattaaaaat
    34621 tcctcctatt ttatcaattt taaaacttta tatttactaa aattaaagtt acataaaaat
    34681 tatactgtct caaaatagaa acaagcatct ttttcattgt tgaaataata aaaatatatt
    34741 ttatttcgta caaaaagagt gcctcatgaa ctaaaaagtt cattttgaga cactcccatt
    34801 cttttatatt tttacttaca aaataacagt acttattaat tttttacaaa ccttataagt
    34861 atatctatat tatattctta aatttaaaca ctatattaaa aaattaaatt ttgtttaatt
    34921 cttttactgt taaatccatt ttaggatatg gaatcgtaat tccttcttcg tcaaacctaa
    34981 ctttaacctc ttctagtaaa tcaagataaa ttgtccaata atcttctgtc ttgcaccaaa
    35041 ctctagttgc aaatttaaca gcattatctc catgttctac tactcctata aatggttctg
    35101 gctcatttat tattaattta tgattattaa caatatcttt aagtactctt ctaacatgta
    35161 gtatatcatc ttcataccct acactaaaag tcaaatcaac tcgtctagta ttctttgctg
    35221 aatagtttat tagactatca tttgatacag aaccattagg tataagtatc tgtttattat
    35281 ctggagtaac caattgtgta taaaaaagtc ctatttgttc aaccgttcct ccatggcctg
    35341 ctgcttctac gtaatctcct actttaaaag gtctaatcaa tagtataata aatcctccag
    35401 caaaatttga tagactacct tgtaaagcca aacctattgc tacccctgct gatgctatca
    35461 cagcagcaaa actggacaaa ctcattcccc aatattccag aacaatgatt ataacggcaa
    35521 ctttaagtac actcaaaaga agagacttca aaaaccttct taaagttaaa tcaatatctc
    35581 ttttactaag tactaacata acatggttta caaatttttt tattatttta aatccaatag
    35641 aaagtataag tatacctatt ataagtttta ctccgcttgt agtagcccat tctagcagac
    35701 tttccattat acctcccata tccatcttat taatgtctaa attatttaaa tctaacttgc
    35761 ttatatcatc tattttcgtc gtatccataa aaaactcctt accttatatt ttagaaccaa
    35821 tattatagtt taaagttctt attttctatt taatataata caataccatt tgtacatagt
    35881 aaacaagatt tatattccaa ttatacatct taggaaaatg caaactattt gttatatata
    35941 tgcaaaatat aatactaaat taaactatta ataaaacact atatagcctt acaatcatca
    36001 ttttttaccc ctatatgaga tgatactata gttataatac ttagtactat aaatactaca
    36061 aaaccttttt tatatccatc aactatatta tatgaattta cttgtaaaga gatagctata
    36121 atcaaattaa taattccact tcctataaat tcaaaagtat taataacact agttgccagt
    36181 ccagaataaa tttctttatt tttatacttg gcatcattaa aaacctgtag atgtgacata
    36241 actatacatc ccataataaa gaatgatatt ggtaaaacta taattggtgg ttttacctgg
    36301 tatacaacta caattacaat ccatataaac aaatctatca ttgctccaaa ttttattata
    36361 ttaaatttgc tagaccttat tttagcaaat acaaaatcca taattattga accaaatata
    36421 aatccatatg taaaaaatga tactataaac gcagaaaaac tcttactcac accataaacg
    36481 tccattatgt atcttactcc ccacaagctt ataaatgcac ttgtaagacc aacaaaagag
    36541 aacattatca ttgaattata ccatgttgat ttgttcttta taacagactt aataccatca
    36601 acaatattaa ccttctctga cttttcatag ggttcagtat ctacattgaa tccatactct
    36661 tttggtgtat ctctaacaat aatatatata aaacatccaa ttacaacacc aattataccg
    36721 attaataaaa atgaatttct ccaaccaaca agttcagaca aaaacacaag tggaaaagta
    36781 gcaagaactc cacctaaatt tcctacaaat gaaaatttag ctgttgcaga agcaaattct
    36841 gacttattaa accatctccc ttgaattttt aaaatacaaa gtaatattac tgatgttcct
    36901 gcccctacaa ttacacgaga aatatatgct aattctatac tttgaatcaa accaaataaa
    36961 attgacccta aacttgccat tattattcca agactgctaa tttttcttgc tccatattta
    37021 tcaatcaaaa ttcctgcagg tatttgcata aatgcatatg catataggca aaaggatgct
    37081 atatttgaaa tttgtataga attaaaacct aactcatttg ctaaattgtc tgaaacaaca
    37141 gcagttgaca tccttaaaaa taatactaaa acaaatgaaa atgtaattat accccaaaca
    37201 ctccacttag cctttgtata acttttcata tcttctccct caaatccata tatttttaaa
    37261 ttttcttgat ataacttata taaagtgtat agttattctt acaaattttt acacgcaata
    37321 tcattataca acatatgtat atatttaact agaaataaaa tactattcat attatataca
    37381 tgaattaact acataaataa taagagccat cttttaacat tactcaaaag atggctccat
    37441 ataaaaacat ttattctaaa tttaattttt tatctaatat aaattttgta gcgaaaaata
    37501 acgctattgc aactacaaga ctaccaagta ttgcaagcat aaaactagga ctctgataaa
    37561 gatgatatac catatttgta tcttcatttg gtaagatgct tccaactgca tctccaacaa
    37621 ttgatattac tatatttata gctataaaag aagcaaatgc cactatgttt ctatgtttat
    37681 tgaattttgg taattgtccc attgagattg atgtatataa taacaaaata aatgatgaat
    37741 atgaaataaa cattactact actaataata aaatatctaa aaagttttca gaaatccatt
    37801 taaaactagt gttaaacaaa tctaaaattt caggtagtaa aattccagaa gtaccaaaaa
    37861 gcataagaac tccaaaagtg aatactgcaa ctataaaact aagtacagcg tatataagtg
    37921 ctgttataca tttagataat ataagtgaac tagtgctaac aggaagagta aacattaaat
    37981 aaccttcatc ccccaaaaga ttttttctaa acctttgaat tgtaactaca atagttaaaa
    38041 ctcctaatgc cataaaaaga cttgttaata ctagtattcc tattccctgt acctgaagta
    38101 tatttgtatt cataaatatt ccattaaata cagctactat caatatggct atatataacg
    38161 gtataaatat tcttccactt gcttttaact catattttaa taatttacct aacatttaaa
    38221 ttcctcccta aataatgcat ctatagattt tcctttttct tctcttattg cttcaacgtc
    38281 tccttgtaac actatttctc cttttgatat gaatattatc tcatcacata tgttttctat
    38341 ttcacttatt aagtgggtag caatcagtaa agtactatct tcagaataat tttttaatat
    38401 agtcttaagt atatatgacc ttgcagctgg gtcaacacca cctataggtt catccaatat
    38461 atatatgctc gcatttcttg acattactaa aataagttga actttttctt tagttccttt
    38521 agacattgtc tttagctttt catttacatc aatatctaag cttttaatca tctcatttgc
    38581 tcttctaaca tcaaaatcac tataaaaatc atagaaaaac ttaagtaaat ctgatacctt
    38641 catccaatca tttaaataag ttctttctgg taaatatgaa actatttttt tagtatctat
    38701 ggatggtttc attccattta tcataatttc accattatca ggctgtaata atccattcat
    38761 tagcttaatc attgtacttt taccacttcc attaggccct aacagcccga ctatttttcc
    38821 ctttggtata tttaaatcaa tattttttaa aacattctta gttccataac tcttgtttac
    38881 acctttaaat tcaactattc catcttgaga aacattgctt atatttttat ccattttata
    38941 attctccttt cagattttct gtaataagtt ttagcatttc atctctatca tatccaagct
    39001 tttctaaggt tacttttaat gtatttatct ccacattagc tatttcctct ttcatagctt
    39061 ttattttttc cttatcatct gttacaaatc ttcctttagt tctctgacta taaactaaac
    39121 cttgtctttc aagttctgtt agagcctttt gcattgtatt tggattaact tccgcatctt
    39181 ctgctagtgc tctcactgtt tctaatttag atcctatttt aatttcccct gaaattattt
    39241 ttaactttaa atgttctact aattgtatat atataggttt gttattatca agttcccatt
    39301 ccatgttcat gcctccttat tatattattg tattaattta ttaatacaat aatataataa
    39361 tacaaagtat atgtcaatta ctttttagta aaagtataaa aaatttcaca aaaaaaatga
    39421 acactacatt agagtccatt tttaacacaa tttattaaat tctttatatt acagattaat
    39481 taccctctta ccaagttgga tacaattaaa tgttcaaatt cttcaattgg cattggcttt
    39541 ccaaacaaat atccttgtgc catatcacac tttacttctt ttaaaagttt aaactgttct
    39601 tctgtctcaa ctccttctga tatgactttc atacccaact cttttgccat ttctattact
    39661 ttagtaacaa ttattttgct tcttttacta ttataagttt ccataataaa tttttggtca
    39721 agttttaaga tatccatagg aatttctttt aacatattta atgatgagta ccctgaacca
    39781 aaatcatcca tagatattaa aaatcctatt ttttttaaat tattcattat atcaattaaa
    39841 atatcaaaat tatcaaatat gatactctcc gttaattcaa gttctatata ctttgcaggt
    39901 atattgtatt tatcaattat atttttgtat gtttctataa aattattgca atataaatgt
    39961 attcttgaaa cattaactga aacagtaact ggatttattc ctgcatcaat ccattttcta
    40021 aggtatatac atacttcttc aagcacatat aaatctaaat ttataacaaa accgtttctt
    40081 tcaaaaaatg ggataaattc atttggaggt attagccctt tatcaggacg tttccaccta
    40141 acaagtgctt ctgaaccttg tatctctcct gtattaagat ctatcttagg ttgtaagtat
    40201 ataataaact ctttattttc taaacttgaa aacattgtat tttccatctc tttttctttt
    40261 atctcttggt catgaatctt attatcataa aatgcatatg aacttttatg tcctcccttt
    40321 gccattttat gaggtatatt agccctatct atgattgagt tgatatctct atcttctgga
    40381 agtataaaat agattccaca acttaaaact aacttatact tttcttcttg attattatta
    40441 aacaagtcta attcctcaaa tatagttttt aacctctttg ttatatcttc ttcttctata
    40501 tatttcataa ttataataaa attatccgca gatattcttg caaacatttc ctcttcaaat
    40561 atattactct taataatttt tgttaaatta cgcaaaattt cattcccaac ttcatatcca
    40621 aataaatcat ttatatattt aaatttatca atatctatat ataataaagc atactttact
    40681 tgagtatttt tacatagtat tttgttagca tcaattacaa atttatctat agtatttgct
    40741 cctgtaagat tatctgtata tgctatcttt tgcaatttaa ttttaaaatt taatttgtca
    40801 tatattatat agaatataag catcatacat attatagcca taataagtat tatttgtatt
    40861 gcatatttgc taataaactg attattagac acaatagact caaagttatt tatggagttg
    40921 tttttataga gtttgcataa agaatagact ggtgttgtgt atgtaatttg taaaattata
    40981 caaataaata aaaaaaacat caattttttt tgtaaattcc tcataaaaat ttcctcccat
    41041 taaaacacct ctattttatt agcaaataag tacatctcaa aagttt
//
LOCUS       DAEPOG010000014        39443 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEPOG010000014 DAEPOG010000000
VERSION     DAEPOG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.014"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(209..1060)
                     /locus_tag="KR432_000641"
     CDS             complement(209..1060)
                     /locus_tag="KR432_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0639196.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(1086..2024)
                     /locus_tag="KR432_000642"
     CDS             complement(1086..2024)
                     /locus_tag="KR432_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0639197.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(2127..2777)
                     /locus_tag="KR432_000643"
     CDS             complement(2127..2777)
                     /locus_tag="KR432_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0639198.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            complement(2927..3115)
                     /locus_tag="KR432_000644"
     CDS             complement(2927..3115)
                     /locus_tag="KR432_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0639199.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(3222..4406)
                     /locus_tag="KR432_000645"
     CDS             complement(3222..4406)
                     /locus_tag="KR432_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0639200.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(4548..5465)
                     /locus_tag="KR432_000646"
     CDS             complement(4548..5465)
                     /locus_tag="KR432_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBH0639201.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(5487..6215)
                     /locus_tag="KR432_000647"
     CDS             complement(5487..6215)
                     /locus_tag="KR432_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0639202.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(6411..6611)
                     /locus_tag="KR432_000648"
     CDS             complement(6411..6611)
                     /locus_tag="KR432_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639203.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(6736..7116)
                     /locus_tag="KR432_000649"
     CDS             complement(6736..7116)
                     /locus_tag="KR432_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0639204.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(7389..8747)
                     /locus_tag="KR432_000650"
     CDS             complement(7389..8747)
                     /locus_tag="KR432_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0639205.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            9195..9647
                     /locus_tag="KR432_000651"
     CDS             9195..9647
                     /locus_tag="KR432_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBH0639206.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(9804..10421)
                     /locus_tag="KR432_000652"
     CDS             complement(9804..10421)
                     /locus_tag="KR432_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH0639207.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            10970..13630
                     /locus_tag="KR432_000653"
     CDS             10970..13630
                     /locus_tag="KR432_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBH0639208.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            13855..14178
                     /locus_tag="KR432_000654"
     CDS             13855..14178
                     /locus_tag="KR432_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0639209.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            14193..14576
                     /locus_tag="KR432_000655"
     CDS             14193..14576
                     /locus_tag="KR432_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0639210.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            14672..15085
                     /locus_tag="KR432_000656"
     CDS             14672..15085
                     /locus_tag="KR432_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0639211.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            15192..15650
                     /locus_tag="KR432_000657"
     CDS             15192..15650
                     /locus_tag="KR432_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0639212.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            15675..16217
                     /locus_tag="KR432_000658"
     CDS             15675..16217
                     /locus_tag="KR432_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0639213.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            16253..16780
                     /locus_tag="KR432_000659"
     CDS             16253..16780
                     /locus_tag="KR432_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBH0639214.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            16890..18284
                     /locus_tag="KR432_000660"
     CDS             16890..18284
                     /locus_tag="KR432_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639215.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            18564..19685
                     /locus_tag="KR432_000661"
     CDS             18564..19685
                     /locus_tag="KR432_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH0639216.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            19692..19826
                     /locus_tag="KR432_000662"
     CDS             19692..19826
                     /locus_tag="KR432_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0639217.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            19827..20048
                     /locus_tag="KR432_000663"
     CDS             19827..20048
                     /locus_tag="KR432_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639218.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            20368..21006
                     /locus_tag="KR432_000664"
     CDS             20368..21006
                     /locus_tag="KR432_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBH0639219.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            21083..21271
                     /locus_tag="KR432_000665"
     CDS             21083..21271
                     /locus_tag="KR432_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639220.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            21292..21789
                     /locus_tag="KR432_000666"
     CDS             21292..21789
                     /locus_tag="KR432_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0639221.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            21805..22308
                     /locus_tag="KR432_000667"
     CDS             21805..22308
                     /locus_tag="KR432_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0639222.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            22421..22810
                     /locus_tag="KR432_000668"
     CDS             22421..22810
                     /locus_tag="KR432_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0639223.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            22797..25229
                     /locus_tag="KR432_000669"
     CDS             22797..25229
                     /locus_tag="KR432_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639224.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            25229..27364
                     /locus_tag="KR432_000670"
     CDS             25229..27364
                     /locus_tag="KR432_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0639225.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            27361..28368
                     /locus_tag="KR432_000671"
     CDS             27361..28368
                     /locus_tag="KR432_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0639226.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            28384..29277
                     /locus_tag="KR432_000672"
     CDS             28384..29277
                     /locus_tag="KR432_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0639227.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            29490..30347
                     /locus_tag="KR432_000673"
     CDS             29490..30347
                     /locus_tag="KR432_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0639228.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            30425..31006
                     /locus_tag="KR432_000674"
     CDS             30425..31006
                     /locus_tag="KR432_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0639229.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            31110..31955
                     /locus_tag="KR432_000675"
     CDS             31110..31955
                     /locus_tag="KR432_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639230.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            31952..32635
                     /locus_tag="KR432_000676"
     CDS             31952..32635
                     /locus_tag="KR432_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639231.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            32632..33330
                     /locus_tag="KR432_000677"
     CDS             32632..33330
                     /locus_tag="KR432_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639232.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            33342..34253
                     /locus_tag="KR432_000678"
     CDS             33342..34253
                     /locus_tag="KR432_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0639233.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            34306..34446
                     /locus_tag="KR432_000679"
     CDS             34306..34446
                     /locus_tag="KR432_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639234.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            34561..34752
                     /locus_tag="KR432_000680"
     CDS             34561..34752
                     /locus_tag="KR432_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBH0639235.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            34776..35006
                     /locus_tag="KR432_000681"
     CDS             34776..35006
                     /locus_tag="KR432_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639236.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            35272..35562
                     /locus_tag="KR432_000682"
     CDS             35272..35562
                     /locus_tag="KR432_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639237.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            35562..35696
                     /locus_tag="KR432_000683"
     CDS             35562..35696
                     /locus_tag="KR432_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0639238.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            35677..36858
                     /locus_tag="KR432_000684"
     CDS             35677..36858
                     /locus_tag="KR432_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0639239.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(37198..38010)
                     /locus_tag="KR432_000685"
     CDS             complement(37198..38010)
                     /locus_tag="KR432_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0639240.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(38086..39321)
                     /locus_tag="KR432_000686"
     CDS             complement(38086..39321)
                     /locus_tag="KR432_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH0639241.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
BASE COUNT        12810 a         7026 c         7052 g        12555 t
ORIGIN      
        1 tttttattta ttatatgtta ttatctttaa tctatatttt aatagttctt aatagtattt
       61 attttgtcta atacattaat tataacttaa atattttata tattaattta atatattaat
      121 tcattcaatc tactggttta atccattttt acagtatata catctacata ttataaaaag
      181 caaaattata ttttctaaat attttatgtt agattataat attaaattct attatctctg
      241 gtatattaaa aaatctaaat ggtaactttg tacttccaag acccgaactc acatataaat
      301 gatttccatt tatatcataa aaccctttag tatatttcct cccatatgga ggagtaacta
      361 atgctccctt aataggtaat cttatttgac caccatgact atgcccagat agaactaaat
      421 tcattgtatc tttactaagc atatctacta aatctggttc atgtgctaac aataaattaa
      481 aatttttatt atctatctga ctctccagat attttatatt aggatttcca tttaatattt
      541 catcaacacc taatatatta atatatttat catcttttaa ttttattttt ttattttcat
      601 ttactaaaag attgaagttg gaatttttca taatctgcct ataataccta ggaagtttat
      661 acatataatc atgattgcca aatactgaaa attttcccaa cctagcattc atacttgata
      721 aaatagttgt aatttcatct acacttggat ttttattgga gtaatcaatt aaatcaccag
      781 taaatactat tatatctgga ttcaaagtgt taatcttatt agctactttt ttgagattct
      841 tagttgaata aaaactacct atttgagtat ctgaaatttg aactactttt acactttcag
      901 atttactctc aattgtatca ttttttatca atgctccatt ttcacttata ttatattttt
      961 tcacaattaa tagactcggc tctatcgcaa ctgagtatat aaaaatcaaa ataaatatta
     1021 agataaaaaa ttttaaaaaa tacttaattt ttgagttcaa aatatctacc tacttattta
     1081 tttttttaaa ctatttgcat tacagactca actttgttca tttttctgtc atgcattttt
     1141 ggcttatttg ctgaaaacca tttatatgtt gctgtaagac cttcttttag taacacattt
     1201 ggtatataaa gcccatgttc aataagctta tttatatcaa gattaaagtc tatatttcta
     1261 aatgggaaat acgttctttc tctaaactct actttttcca tatctacata ttttattata
     1321 ggttctttgc caataatttc tccacatgta tatataaggt catcccaaga aattaactgt
     1381 ggatttgtca cattataggc ctctcttaca tgaggatttt tcattatgct ttccaatacc
     1441 tttaccaaat ccccaatata tataaattga ttactaactt gttttccttt tggtactggt
     1501 atcttttcat ttttttctat tttttcaaag aaataatact ctctatataa attattattt
     1561 tcaccatata tgtaagttgg tctaataatt atatatggta tagggctatt tataataaaa
     1621 tcttcagcct cttttttatt taacccatat tttccccagt tttcattttc acctttctca
     1681 ttttcttctt ttatatttct tccactatct ttatatactg ctcctgctga caatactata
     1741 tactttttta agttatccat gcttataaaa tcaatcagat tacttacatc ttcttttgta
     1801 tatgctgtca tatcatatat gtaatcatat tttcttcctg tgataatgtt ttccatatct
     1861 tttcgaactt ttctatcaca aattagatgt tctttaaaac cattataatc tatttccttt
     1921 ttaccatttg taagtatatc aatttgatac ccacatttaa tcaagcgctt agcaagtgca
     1981 gaacctatga agtcgcttcc acccataatt aaaatagatt tcattttaag tgcatcctcc
     2041 tcttttttaa atgacttcct taactataat atcatatttt taaatatgcg acaccatttt
     2101 gtaatatttt tgtctataaa agatatttat aacaaaatat cttttataga ctctacattt
     2161 tcgacaatat aagtaggttc actttcacta agctcttcaa aagaaccata cccatacaat
     2221 actccaatag aatctatacc tattttttta gctccaatta tgtcatactt tctatctcca
     2281 accataataa ctttatcttt atcttttaca ttacataggt ctagtacata ttgtataact
     2341 tcatttttat tcactcttgt accatcaaga ttgctacctg ctatatattt aaaatatcta
     2401 tctatatcaa aatatctaag tatagtttct gcaaaaactg ttggcttaga agttgctact
     2461 aataatattt taccattttt atagagcatt tctaatatct ctttcatatt ctcataaatt
     2521 ttattttcaa atatcccttt atcagcaaaa tattctctat acttttcaac agcttcttta
     2581 gcttttttat cttcaaattt ataatactcc ttgaaagtat catgtaatgg tggtcctata
     2641 aattggtcta agttttccaa atcttctttt atgccaaagc tatttaaaga atactgaata
     2701 gactttgtta ttccttcttt agggtctgta agtgttccat ctaaatcgaa taatactatt
     2761 tcataatttt ttttcatatt tcagtcctcc atatttgtaa aaagtaatat cttacatatt
     2821 aagtttatct attttaaaaa gctatagtaa tagaaatttc taatactata gcttaagtac
     2881 ccaattaaat cttatatatt tattttaaaa ataaatttaa acagctctat ttaactgtta
     2941 tagctgaaac tggacatcct tcttcggctt ctttacataa atcttgtaaa tctgctggta
     3001 tttctccttc aattgctact gaatgccctt catcgctcat actaaaaacg tctggacatg
     3061 ttcctataca tgctccacaa gctacacata catccttatc aacaaatgct ttcatactaa
     3121 aatacctccc ctaaaattat tattttttaa ttgttgtttc cttagatatt tatccaatat
     3181 ataccaattt aaacatatat ttattatttt atcactattg tttagtttat tatagtattt
     3241 tcgtatccat gtctataaac ttcactgctt ttcatccatg attttacagt atattgcaat
     3301 cttaattttt tatttaaaac aagttcatta ttattgtagt cttttataat atcattacac
     3361 tcctccattg tgaatattgt aaaatcacct tctcctccaa tctttattct gcctttattt
     3421 ccatgtaaat cgaaaatctt tgttgggtat tcagttactt tttctataca cttttctagg
     3481 gatatcccaa gttccataat tttatttata acatttaaaa gagaatagtt tacgtctttc
     3541 atgtttttta tatgcatatc agtacttatt aggtcaggct ctaaaccttg ctttattgct
     3601 tttttaaaaa ctttaaaatc aaattggtcc actccatgac atacatcgaa caatactcct
     3661 ctattctttg catttattac tgattttctt aatgtgccag attctttaag gattccattt
     3721 gtactatttc catatactgg tgtaaccaca tctccttttc ccaaaatatt cattacttca
     3781 tctatatatg atggatactc tcctatatat accactaagg gtaaatttaa ttttttagca
     3841 acttgctttg atttcttaat tatatctata ccactttcat tagattgaaa tttactcgct
     3901 tttactttta taccctttat cttttctata tttttttcta caattgactc aaccttgtca
     3961 aaatttataa catctaaaga agttgattca ttcaatatat ttagtccctc atttgtaaca
     4021 tttaaaaaag aatatacttc tgttttacac tcatccatat actttttttt aaaatcatag
     4081 tagtttgagg gaccagctgt acctgcatca aatataactg tagaaccttt ttcaattcca
     4141 attgaatcag aatccatacc tgtaggtaat tttctcttat aacaatgggt gtgaacatct
     4201 ataaatcctg gggaaatata catattatcg gcatttatta tctcgaactc tttatttgta
     4261 agtaattttt cttttcttat tttctcttta aggttcaatc caatagcata tattttccca
     4321 tgtaaaacca agatatctct tttttcaaat ttgataccat ctgttggaga tattattgtc
     4381 ccatttctta taagtaaatt tttcatttat tccacatcct aattacaata taatttctct
     4441 aatagctttt cttacataaa gaaaataagc ctaaaaataa aaatatttta gacttattca
     4501 ctatattata ctatattaat tatattaaat taaataaaat ctttaattta ctttaagaag
     4561 caattaagta tttgttgctc ggcttcttct tcagtaagac ctaatgtcat catctttagt
     4621 atttggtctc ctgcaatctt tccaatagct gcttcatgta ctaaaagagc atctggatga
     4681 tttgcagcaa tttcaggtat agcactaatt tgagaatttc ccataattat tgaatcacat
     4741 tgaacatgtg cagaacaaag ggaatcccca ataacacaag gattaaactt ttgcttagaa
     4801 ttatcttgcc caactgtacg agaaactact tgagcagaag agttttcacc ctctaagata
     4861 accttcatat tagattctgc aacttgatta ccatgagtta ataatttctc aaatacaact
     4921 attttagctc ctttacctag gtttgcatcc atttctcttt tagttgaatc tacaccctta
     4981 atttgaattg tttccatttc acaatatgaa tcctcatcca tattgacaat agtaactgga
     5041 tttaaaattc tctttccttc tccatcacct tcaccataat gtttttctac atattttagg
     5101 cgagctcctt tacgtaagaa aaatctatgt ataccattat gttcactatc atcacaacca
     5161 cagttatgta taccacaccc agcaattata acaacatctg cattttctcc aatttcaaaa
     5221 tcattatata ccatatcctg tacaccagcc tctgttacta ttactggtat atgtacattt
     5281 tctccttttg tatttggctt tactataata tctattccag gtttatcagt tttaggaatt
     5341 atagttacat tttcagttga atgtctctct actccttgcc catttttacg tatattgtat
     5401 gctcctaatg gagttccacg aagttcagca actttttcta aaagatttaa atctattttg
     5461 ttcatttaat ttgccttcct ttctaattaa ttcatgtctt tcattaattc acaacttgaa
     5521 ttaacttgac acatgatttc aggtaatata gcctcttttg ttccatggct ttttactgaa
     5581 ccatcttgta gaacaattat ttcatcagct aactctaata ttctttcttg gtgagaaatt
     5641 atgattatag tctgattaga ctcttcatgt attttcttaa aactttcatt gagttttcca
     5701 aagctccaaa ggtctatccc tgcttcaggt tcatcaaata tagaaacctt taaatctcta
     5761 gcaaaaagag ttgctatctc aattcttttc atttcccctc cagaaagaga gttatcaact
     5821 tcacgattta aatagtcttt tgaacacaat ccaacatctg taaggtattg gcaacataca
     5881 tcagtagaca atggttttcc atgagcaagt gtaagtaaat tttcaactgt catacctttt
     5941 attcttggtg gttgttgaaa tgcgtatcct attccttttt ttgctctttc tgtaatacta
     6001 agatttgtta tatcttctcc atctaaaatt atctgaccac tcgtagcttt ttcaattccc
     6061 ataataagct tagcaattgt agattttcct ccaccatttg gacctgtgat aacaataagt
     6121 tttcccctct caaatgaaag gctaacatca tttattattc ctagttcctc attgttagac
     6181 tcaacattaa aggaaagatt ttttatttca agcataatac ccctcttttc tataaaatat
     6241 tacattaata tccattcttt tattatatca gtaattttta tatatcagca actctaagca
     6301 gataaaatgt atattaaata ataaaacaaa gttattgaaa agtaaaaaag atgagtttta
     6361 tgtaaactca tctcagaaat ttagtatatc aacttatatg tatttaaaat ttaaccttct
     6421 atatactcta atattatttt ttgattatct tttaacataa tttcttcttt tttagattct
     6481 attccattct tttttatctt tacctcagct acaaaggcat cattaccttc aaccatttct
     6541 aatagttcat acgctttttt acgacctatt ttagttgcta tatcttttag tactgccatt
     6601 ttcttaaaca ttttatcacc tctactcaaa atttacactt aatttttaat tatataattc
     6661 taatcctaga ctagtttata ataattattg tctctaatta tatcagatat tgagtaaatt
     6721 taatactttg taaaattatt tatcctctct gactaaataa tcatttccat cttggtcttt
     6781 aaaagaaaac atccttccat atggcatctc cattaattcc ccaacttcaa cctcgttatt
     6841 tgccatttgt tcataagttc cattaatatc tcttgtactt aaaattattg atggatgaga
     6901 tacatttatc tctttgtttt gcttcatcat tagattttta tcatacaaca caaatgttgt
     6961 aaattcattt ttacttggtg ctacttccac ccatttcata tttggaccca tttccttttc
     7021 ataagttact acaaagttca atttttctgt ccaaaacaac tttgcatcct cttgattatt
     7081 tacatagaga gttatttttc ctattttatt tatcataaca atacccacct ttattattaa
     7141 ataaatacct aatatttttt atatttaata atatacccat atatattatt ttctatagat
     7201 atatatttct aatatttatc gccaaattat gtaattctgc tcaatatata atttataata
     7261 tccatactat tatagccttt atttatgtaa tttaataacc ttatatatca tacttataat
     7321 tatgataata actacataaa aaatcacttc taaatagagt tatccatcta aaagtgattt
     7381 catatattct atgcaacttt gtgttttagc cactttgttc ctcttaatct taaacaatat
     7441 aaagtccctc taacaagcca atcagtatac atacctatcc aaattcctaa cactccaaat
     7501 tctaatacaa cccccaatac gtaacctgta aaaattctaa aaatccacat acctattact
     7561 gcagtcatca ttgtatatct agtatcaccg gctcccttta gccctgctga taatacaaag
     7621 gatataggcc atattaccat agctaaacaa tcacttcgaa ttaattgtgt tgagatgtca
     7681 attacactag cattttgagt atacatactt gaaatccagg gtgcaattgg tataaatata
     7741 gctcctacta taaataaaca aattgttgca aatttagtta aatataataa agtactctta
     7801 gctcctttaa tatctccacg tcctatatac tgtcccacca aagttgtagc agacaaagct
     7861 aaagcattcc caattacatt tattatggat gcaattgaca ttcctattgc attggatgca
     7921 attgctgatg ttcccatagt aactataaat acttgaacaa tcaatttacc tgcattaaaa
     7981 agtagttgtt caactccagc tggtatccct atattaaata tagacttttg agtttcaaca
     8041 tcaatcttaa atggaaatat tttacccata gatataaatt catttccttt aaataaagct
     8101 cttataatta ctatacatcc aattattcta gctatagcaa tagatatagc tgcacctgtt
     8161 atgccatagg atggtgtttg caaatttatt cctagaggat ttaaatcaat accatatata
     8221 agtatatatc ctaatgttac atttactata ttcataaaca tagtcacata cataggtttc
     8281 tttacatctc cacaccctct taaaatacca tttgctattt gttgtgtcgc aataaatgga
     8341 tacgtaaata atgtaaactc tatgtataat tttgcatttg attttaccaa ttcttctgct
     8401 gaaccataaa ataaattaat tacaccagtt ctaaaaaccc acatcaatat tgttattgta
     8461 aaggataata ttactccaga cactagagcc tgcttaatag tttcattcac cttttcacta
     8521 tctttttttc ctatttgttg agctacaacg acagtagctc caacagataa tgcagcaaat
     8581 acagatataa atatattgtt tattgaatca accattccaa ctgcagatac agcttcttcc
     8641 ccaatatgac ctgccattat tgtattaaat actcctaaaa gcatgacaaa cgtctgctct
     8701 atcattatag gaattgctaa ctttataaca tttttcctta taaacatccc tattcctctt
     8761 ttccctcttt atataaattt aatttttaat tagtttatcg aactgtaaat tgacaatgca
     8821 aagtttagaa ttgtattgtt cgcattataa tatactgctt tcaaataata taaatcttgt
     8881 ttaatatcat ttgaacacag tatatcttta taattatctt atgtttatta ttttttatgg
     8941 ataccacatt taaaatattt attaatttaa tataaatatt ttccgttatt attaaaagta
     9001 gctatatttc gttaatattg tatcactatc gtgtagcaat aacaataagt acattttata
     9061 tattttttat aatattattt aatcatccaa acttttacca ctttttcaat atataaaata
     9121 ttacacttta gatgtaacaa aatcgcatcc atttaaatat tgtattttat agactaattt
     9181 taggatgtga tactttggtt aatttaaatg caaataatct aaatgtaaat atggtttatc
     9241 ctatttcttc cacagcaccc atacgtttca gaagatatac tgtttcatat ttagaaaaca
     9301 tggaaaatat ttatttaact atagcaacat actttgatac acttggttta ggcacaatga
     9361 atatcgacaa agtttatggt caatgggctt ggtttactga taatatatat gatttaaatc
     9421 tatttgtttt tgtaggaaat tatccttatc aaattgctaa aaacagatat gatacatttg
     9481 tagatatttt gccagtagct attacttcta ttgtcaatgg agatagagta tttttaattt
     9541 caaatccttg tttgttaaat tcaaaaatta cagttagatt tatatctagt catcctactc
     9601 ttaatagaac agttgattat ggcaatatta gagattttat tttttaaaac tgcaacatga
     9661 tgtgctatta aaaaataccc taaatttaat gatattatta aataactaga atatttttaa
     9721 ttggcacatc atgcctagct attataaatt cataatatta ttttacttaa gataagtact
     9781 gtattttatc tacttgtata tttttattta cctagtttct cagttctata tcctatttct
     9841 ttattagcta gcttataacc ttcttgtatc atttcttctc tcgttaatgc actccctaat
     9901 aaattgtcaa tactcagttg ctctctatca ctaaaatact tatccaataa tgattcctct
     9961 atatcttcct gatatgccaa atttccttca tgcattatat attttacatc gaaaccttct
    10021 ttatttaata cttttcccac caaaatactt ctatgacatc ttataggttc ttgcatagct
    10081 cctaataaaa ctatcctata acccatttga caaccctttt ttaatctttc tattccgttt
    10141 aaaaaaatat cttcttttgc tactttctca aaatctgcat aaccttcttg cgtatataca
    10201 tctttattat ttcttttagc tccaaattct tttcccatgt atatatatat aaatccggaa
    10261 tttctaagag attcgttaaa tgcctcttta ttgtattgaa tattatattt agaatatgga
    10321 gtttctctaa tgtcaactac acaatttatg tcatattttt ttatcatatt aataagctta
    10381 tcatatggat aatttgaatg tcctattgcg aatatttcca tataaatacc ctcctattct
    10441 ttgaaattag tatctatata tgtagatacc cgatttactt aaaattaaat aaagtattac
    10501 tttaaatacc caatatcttt ttttcttgat actgtatatt tttttacttt atatccttaa
    10561 aaaaatatac aactttatat ataaaacatc aatatttata tatattttgt atattaaata
    10621 caattattta taaaatcttt gataaaaatt tataaataga gcatctttag aaaactatta
    10681 acagaaaaac acactaaaaa tttgattatc cgctaaaaca ctgtatctat cggcattatc
    10741 aaggagctaa acacatcaaa atactactaa tttactacga ttgaatgagc cttgatacgt
    10801 cctatttccc agatatgcaa agatacggta cagcacatca gtattgaaac aagaaaaatt
    10861 gaatacttca tagactacgg aacgcacaat atcagcgttc cttttctatt tccagccgtt
    10921 cctagtggac ggctatttta ctaccaaaat gaaaggagca tttagaatta tgaaaaagat
    10981 attaaaacga ttgtgtacag gctttcttgc ctttgcaact gtcgttaccg ctttaccgag
    11041 ttcaacggtt catgcgtcta atacacagta ttggacggaa agtaaagagc gtgtcagtat
    11101 cgtagaacaa gtaatgaatg acggttctat cagttctacc tttaatgagg gacatttaac
    11161 cgttgagggt gaggacgctt attgtatcga catcaacaca gcctttaaaa acggctataa
    11221 gaccaaagct gacgcaagta cccgtatgag tgccgaccag atagctgacg tcgccttatc
    11281 tattgagtat gtgaaacagt acacagacat ccacagcgga ataagcagta aacacgccta
    11341 cctattaaga cagcttgttg tctggcagag attaagtgtt cagcctgact ggcaatggga
    11401 taatgtgcga gcttcctatg atgaaatccc aaaagccgta caagatgaag tattcgcagg
    11461 agcaaaagcc tttgtgaaag aaaacaaagg acgttatgac tgtggcggat atatctactc
    11521 tggcgagggt caagagttag ggcaattctg ggcgaagtta gctgtcggaa ataccaaact
    11581 tcaaaagact tccaccaatg ccaatattac agacggcaac gggatttatt caatcgctgg
    11641 tgcaacatac ggtgtgtatt ctgataaaga ctgcacgaaa cagcttacca cccttacgac
    11701 tgatactagc ggaaatacag aagccgttga agtaagagcc actatagtat atatcaagga
    11761 actttctgca ccagcaggat ttaaaattga taaaacagtt tactctttat ctgtggaagt
    11821 tggaaaaaca gcgactttaa aagtatctga tacaccaaaa gtaacagata ccttgattga
    11881 gctattcaag attgacatgg aaacacagaa atccaatccg cagggaaacg cttctttaga
    11941 gggagcagag tttacatgga acttctacgc tggatattac aacaaaaaca atcttccagc
    12001 acagcctaca agaacatggg taacaaagac tatcgctgaa aaagacagcg acggtgctat
    12061 ccattacatt actcgtttag ctgacaaata taaagtatct tgggacagct tctatacgca
    12121 ggacggtaaa aacgtacttc cattaggaac actaactgtt gaggaaacaa aaagtccgtc
    12181 gggttacttg ttagagggta catatatgca ggctgacggt agcgaagaac agataaaagg
    12241 aatgtatctg acacagatta cagaggacgg cgaccttgcc gtattatctg gaagcaatca
    12301 ataccatgtt tctgataaag tcattcgtgg cggtgtgaaa atccagaaac gagatttaga
    12361 aaccaaagac acgaaagctc aaggcggtgc gactttgaaa gataccgcct ttgaaatcat
    12421 ttccttaaat gagaatgccg tcttagttga gggcaagtta tacaagaaaa atgaagtgtt
    12481 aaagacaatc cataccgaca ttgagggtat cgcttctact tctgctgacc tattacctta
    12541 cggaaaatac agattgtctg aacaaaaacc gccagaggga tatttgactg acggtgcaaa
    12601 gccgattgat tttgaaatca cagaaaatgg aaaaatcgtt gatttgactg acgaagccca
    12661 ctctatctat aaccagatta aacgtggcga tattgagggc gtaaaaattg gtgcaggttc
    12721 gcacaagcgt cttgcagatg ttccgtttag gatcacaagc aagacgacgg gcgaaagtca
    12781 tgttgttgta actgatgata acggacaatt ctctactgct tctgattggg cttcccacaa
    12841 gcacaatacc aatgcaggaa aaacaagcga ggacggtgtg tggtttggta cttctgaacc
    12901 agacgacagc aaaggtgcgt tgctttatga tacttacatc attgaagaat tacgttgtga
    12961 aagcaacaaa ggtttcaaac ttattccacc gtttgagatt gtcatttcca gaaataaggt
    13021 tgtggttgat ttaggtacgc tgactgatga atacgaaaaa gaaatcacta tccatactac
    13081 tgctaccagc aaggacggcg aaaagactat tcttgctggg aaagacgtaa ctatcactga
    13141 tacagttaaa ttagacggtc ttaccaaagg gacaaaatat cagttgaaag gctggcagat
    13201 gttaaaagaa gaaaatgcag agcttatcat caatgacaag cgtattgaga atgactacat
    13261 ctttacggct gacagcgaaa ctatggaagt taaaatcgag tttacctttg acgcttccaa
    13321 tcttggcggt aaacagcttg taacttttga ggaattatac gatttaagca atccagacga
    13381 acctatcaaa gttgcggaac acaaggacat tgaggacgac ggacagacag ttactatcaa
    13441 ggaagtacca gaaacaccta ccccagaaga accagagaag ccaaccacac cagatacacc
    13501 tacgaaaaca gacagtccca aaacgggcga caatacaaat atccttgcct ttgcaattat
    13561 gatgtttgtt tctgctggtg ggctggctgg tacatacttt ttcaaacacc gtaagatgaa
    13621 gaaatcataa ggcggtagct gtatgagaaa agaaaaatca cgctctccgc catagcttag
    13681 ataggcagac tatacctatt ctggcttgtc tgccgattac ctgcaagaac acggatagtc
    13741 aagggtgcgt tagcatttat ttacccttta ctatctgggg gcttgctggc actttcctaa
    13801 atgccagaat acacacatca atcaaactta aagtaagaaa cgaggtaaaa taatatggaa
    13861 ttaaaatata tcgtgccgaa tgtggcacag acttttggaa acttggaatt tgcaggggaa
    13921 aacaaagtag accagcaacg tatcaatggg cgttacactc tcgtatcaag aagttacaac
    13981 ttgtattcgg atattcagag agccgacgac attatcgttg tattgcctgc aaaagctggc
    14041 gagaagcatt ttaagccaga gcagaaagta aaactgatta acccacgcat taccgcagag
    14101 ggttacaacg tcaaaggacg tggatttaca aactacattt taatggctga tgatatgtta
    14161 ccagcagaaa gcaaataagg gggtatttga ttatgagatt aacaaatgga attgtattag
    14221 ataacgaaaa aacttttgga gtattgaaat tctctgcact tcgacatgag gtgcgtgtag
    14281 aaaatgagga cggaacgaca agtgatgaaa ttaagcgtcg tacttatgat ttgaaatgta
    14341 gtgtgcagga acgcatgata caagtgtcaa tccccgctga tgttcccgta aaggactttc
    14401 cttataatgc ggaagtggag ttaatcaatc ccgtagctga tacggtggca aatgccaact
    14461 ttagagggac tacggttgat tggtacatca aagctgatga tattgtattg aagaacaaag
    14521 gcggtcaagc tggaaatccg cagaacaacg ctccgcaggg acagcagaaa aagtaagctg
    14581 acaaactagt ttttcattgt aggagatata aacaactgtt ataatatgga tgtggaaaca
    14641 gttaatttag acttataaag gagaaatgtg tatgctagat aaaataccta atgcagagga
    14701 aatgactgct ttggtcggac aatcattata tagtgtatgg aatgaattgt gtgccttgat
    14761 tgatgaaaag tatgaaatgg aatgtctatg gaataaaggt ggcaaagcat ggaattatga
    14821 atataagtat cgtcgaggtg gcaaaacatt atgtgcctta tatgctagag aaaattgtgt
    14881 gggatttatg attatcttag gtaaagatga acggttaaaa ttcgatacat atagagataa
    14941 ttattcaaaa gaagttcaga gaatttatga tgaaacaaaa acttaccatg acggaaaatg
    15001 gctgatgttt gagccaacag atacaacttt gtttgacgat tttgttaaac ttttgaggat
    15061 aaaaagaaaa ccaaatagaa agtagtttat taccacacca ttagcaaatg gatagtttct
    15121 tataatagcg gatatatgaa aatgctgtca tgtggacata aagaaaattg gaagttgtgg
    15181 aggtagcaaa aatgaaatta aaaaacccta tgttggtagt aactgatata gataaatcag
    15241 ttgagtttta taaaaaggtt tttggacttt atgtcattat ggattttggg gcaaataaaa
    15301 ctttaacagg tggattagcc ttacagacaa gtgaaacata caaagagttt atcggtacaa
    15361 gtgatatttc ttttggtggt aacaattttg aagtctattt tgaagaagaa gattttgata
    15421 aatttgctga taggctgaaa gaatatgata ttgaatatgt ccaccctatt atagaacatt
    15481 catggggaca gcgtgtagtc cgcttttatg acccagataa acatattatc gaagttggag
    15541 aaaacatgaa gatagtgtgc aagcgtttct taaatagtgg aatgacacca gaacaagttg
    15601 cagaacgtat ggatgtacct atgaaattta taaatgcttg tatgcgataa attgaaagtt
    15661 aggagactaa tactatgaaa ggatttgaac gcaaaaatca attattttct ctttgcggat
    15721 tgaattgcgg actatgccct atgcttttgg gaaattactg tggcggttgt ggcaatggta
    15781 atcaatcatg cagaattgca aaatgtagtc ttgaacatgg acagattgaa tattgctatg
    15841 aatgcaaaca atatccatgc gaaaaatatc aacacattga tgattatgat tctttcatca
    15901 cgcataaacg gcgaaaagca gatttagaaa gagcaaaaaa cataggcatt gagcaatata
    15961 atcttgaaca gcaggaaaaa acacaaattc tttcttactt actttctaac tataatgacg
    16021 ggcgtagaaa gactttcttt tgtgtggcag tcaatttatt agaactttca gaattacaag
    16081 aagcaatgaa acaaatacaa tccaatgacg aattgacctt actaccattt aaggcacaat
    16141 gcttgtatgt ggtggaggtg tttcagaaga ttgctgaaag gagaaatata aaactgaaac
    16201 ttttcaagaa aaagtgattt tcaaatttga aattgtaaca aagattattt atatgacgaa
    16261 tgggggaata cctttggaaa cagtagagtt aagatttcaa tatactcaaa gtgaatatat
    16321 aaaagcagaa aggcaatatt taatttctag taaaactata cataaatatg atattgcttt
    16381 agtcgctgta tttttgttgc tgtcagtcgt ttatatgctg ttctcctctt ttagcgtatt
    16441 tagtatttta atatttggat tgataattgt tgtaactgca ttgggaagct atttatatat
    16501 cctaatgcct atactgaaat tcaaacaaac tgcaaaatat catgaggaat atacgctggt
    16561 attttctaag gaaaacatta aatttaaaac acaaagcatt gactcagaag tgaagtggga
    16621 catttactct gcactatggg aaagccatga tttttattac ttaattcaag caccacgatt
    16681 atatacgtta attcccaaaa gaatttttaa agacctaaac gaaaaacagc tatttgaaga
    16741 aattgcacaa tcaagagtta aaacaacaaa gcatatataa tgaacatacc ctacaatgta
    16801 acaacttcat attcatacac acaaagcggt tagtctttta agattgactg cttttttcat
    16861 acttaaatta agattggagt gattgattta tgcgaatgat tttgaataaa ggacaccgta
    16921 tcagagccag agacaagaac cttgtctacc acttttccat agggacgctt ctgtttgtat
    16981 ttgtggcggt tctcctgcta ctgaatatca aacagctcat gcgtaccgac tgggaacatt
    17041 tcagcttatt ggacaatggc ttgacgcttt ccccctacaa cttcataacc atattgatag
    17101 cgactggtat ttgtgcattg gtcgcttttc tgtattaccg cttctgttat gacagtttca
    17161 aaaagctcct gcaccgtcaa aagctggcac gaatgatact ggaaaacaag tggtatgaag
    17221 cagataccgt acaagacagc ggttttttta ctgacctgca aagcagatca agggaaaaaa
    17281 tcgtctggtt tccaaagatt tattatcaaa tggaaaacgg attgcttcat attcactgtg
    17341 aaatcacatt aggaaaatat caagaccagc ttttgcgatt agaggataag ctggaaagcg
    17401 gtttgtactg tgagctgacc gacaagacct tgcatgacgg ctatattgag tacacgcttt
    17461 tatatgacat gatagcgaac cgtatttcca ttgacgaagt gaaagcagaa aacggctgtc
    17521 ttaggctgat gaaaaatctt gtctgggaat atgacgtact ccctcacgct ctgatagctg
    17581 gtgggactgg tggcggtaag acctactttc tgctgacgct tattgaagct ctgctacata
    17641 cgaatgctgt cctttacatc ttagacccaa agaacgctga ccttgcagac ttaggtacag
    17701 ttatgggaaa tgtgtatcat accaaagaag atatgattga ttgtgtcaat gccttttatg
    17761 agggcatggt acaaagaagc gaagaaatga agcaacaccc agactataaa acgggcgaaa
    17821 actacgccta tcttggttta ccgccctgct ttcttatctt tgatgaatat gtggcgtttt
    17881 ttgaaatgct ggggacaaag gaaagcgtaa gtttacttag ccagttaaag aaaatcgtta
    17941 tgttgggacg acaagctgga tatttcctta ttgttgcctg ccagcgtcca gacgcaaaat
    18001 atttctcgga cggtatcaga gataatttca atttccgtgt tggattgggg cgtatcagcg
    18061 aattgggtta cggtatgctc tttggttctg atgtgaaaaa acagtttttc cagaagcgta
    18121 tcaaggggcg tggctattgt gatgtgggaa caagtgtcat aagtgagttt tacacgcctt
    18181 tagtcccgaa aggacatgat tttttgcaga ccataggctc tcttgcattc gtaaggcagg
    18241 acgggacggc gacgtgcgaa gcgaaaggcg acggcacgga ctagccgttg ctggtgtggc
    18301 gttagccacg ccagcagata taagcccctc attatctaac agaggggcac aattacgctg
    18361 gaaactggtt caaaaacctt gaaaagtccg ctaggaataa ggattttctt agtttttagc
    18421 tagttgtgat aatcacttga aaatatcaca aaatcacaac aatatcacga ttgggggtat
    18481 gacaactgaa tgatactgat tttctgaaca ccttgaaagc aaaacgcagt gattatggcg
    18541 tatcgcagac aaggcttgca atcatggctg gtatcagccg tgaacatcta agccgtatcg
    18601 aagctgggaa ggtagcactg accgaggata tgaagcacaa actactggaa gcggtggaaa
    18661 aattcaatcc cgacgctccc atgttcctgc tctttgatta tgtgcgtatc cgatttccca
    18721 cgctggatat acagcacgtt atcaaggaca ttctaaagct gaatatagat tatatgcttc
    18781 acgaagattt cgggcattac aagtacaccg agcattacta tatcggggac gtgttcgttt
    18841 acacttcaca agacaaggaa aaaggtgtcc tgctggaact gaaaggaaaa ggctgtcgtc
    18901 agtttgaaag ctatctgctg gcacaggaaa gaagctggta tgacttcctc atggacgcat
    18961 tagttgatgg cggtgtgatg aagcgtatcg acctagctat caacgacaga acgggcttac
    19021 tggatattcc agaactgata gagaaatgca agaacgagga atgtatttct aaattccgta
    19081 gcttcaagaa ctatggttct ggggagctgg taaagcacaa cgaagccaac aaggacggca
    19141 tgggacatac gctttacatt ggttcattta ccagtgaagt gtacttctgt gtctatgaaa
    19201 agaattatga acaatcctca aaattaggga taccgattga ggaagtcccc attaaaaacc
    19261 gatttgagat acgtttgaaa aatgagcacg cctactatgc cattagagag ctgctgaccc
    19321 actacgacgc agagctgacg gcattttcta ttatcaacca gtatatccgc tttgccgata
    19381 aggaagccaa caagcgaaaa agcgactgga aaaccaacgc ccgctggtct tggttcattg
    19441 gcgagggacg accgcccgtg aagctgacga caaagccaga gccttacact ctgcaaagga
    19501 cgctgaactg gttacaaaga caggttgcac cgaccttgaa aatgctgaaa cagatagacg
    19561 ctggcaatca gatggactat ctcaagacca ttgaacagca ggcaaagctg acagataagc
    19621 acaagcagat aatcagacag cagacggctt cggtggacga actgatagaa aaagaaaagg
    19681 agtgattgat gatgtgggaa ttgattaagg acttactgct ggtatcactt ggcatgggta
    19741 tcggcgtggt tctgatgtgt attatgagtg tcggaaagga agctgaccga cacatgattg
    19801 aattaaaaga aagcgaggaa aaataaatga atttcgggca aaatttatat acatggtttt
    19861 taagcaacgc ccagagctta gtgcttatgg cgattgtggt tattggtatc tacttagggt
    19921 ttaaacgtga gttttcaaaa cttatcggct tcttggtggt tgccttagtt gctgttggat
    19981 tggtattcaa tgcaggcggt gtcaaagacg tactcttaga actattcaat aagattatcg
    20041 gcgcatagcc gtactttttc attatggttt gcggatacgc aaacatacct ttcccacttt
    20101 gggagtgggc taaggggaaa gggggtaacg gaatggtaca cagataaaaa tacagcataa
    20161 tgaatttcat tgcttggctc aaacatagtt gatataataa agatatgaaa ataataaacg
    20221 aaagtttttt tgataaggag tgatattgtg aaattgtgta ttgttttatc tccatataag
    20281 gcttaatgga atagacttgt atggagtgaa atgttatcca gagagcctta cattttatca
    20341 aatttcaaaa tgtaaaggag aatgggcatg gaatataaat atacaaaaat tgatttgaat
    20401 aaatggaata gaggtaaatt gtttcaaagc tacattgata atatgcgtat tgttatgagc
    20461 ttaaccgtag atattgatgt aacaaagttt attgaatttt caaagaagca tgatttaaaa
    20521 ttttatccag ctatgatatg ggctgtttca aaggttgtta atgaacatga tgagttcaaa
    20581 tatagttggg ataataatgg gaatttaatt aagtgggatt atatttctcc gtcctatact
    20641 gaatttcata aagaagatga aaatttcaca aagatagtta cagatttttc tgatagcata
    20701 tttgaatttc ataaacgttt tatattagat agggagaagt ataaaacaga acgtgcattt
    20761 gtggaaaatc aatctttgaa cttttttgat gtatcttgtt taccgtgggt aaaatataat
    20821 cattttgatg ttcatgtttt tgacgaggga aaatttcttg caccagttat tacttggggc
    20881 aaatatgaat tagagcaaga aagatatata atgcctctta caatgaatat acatcatgcc
    20941 gtagcagatg gattccattt atccagattt tttaatgaag tacaagaatt gataaactca
    21001 atttaacagc tatatacaaa gcagttagtc tatacgattg actgcttttt ttcataccta
    21061 aatttaagat tggagtgatt gcttgaaaga tatattttcg gatatgcagg caagtatcgg
    21121 ctgtacctac atttcagatt taccacatca taaacgtatg gtgtggcagg aaatgaagcg
    21181 actgaacctt gccgattatc cgcagaaaca attagaagat tttgcaggct atgtctttgg
    21241 tgtcccttac actagcatag ctgaagcatg aaagaaagga cgtgaaaacc tatgaatgaa
    21301 atgcgtgtct gtatcgaaag ccgactacac acttctgaag aaatccgttc cgcatggttc
    21361 acacttccca ttgacgagga agaactggaa gaacagatag gcgttcctat cgacagtgaa
    21421 gattaccata tcgttgaaaa ggaactgccc tttgctgatg aagtcggcga agatgcgacc
    21481 attgaaaaac tgaatgacct ttaccatacc tttgaaagtc tgcctgccga tttacaagag
    21541 gactacgggg agctgatgtg ttactttacc agccttgacg aactgcacca gcaccgccat
    21601 gatgtgatac attattcatg gtgtaaaaac atggctgatg tggcaaggca tttgttggac
    21661 aatgaccctg ctttttctgc catcgaggaa cgcctgaccc gttactttga ttatgaagcc
    21721 tacggacagt atcttgatga taacggagaa tttatagaaa cagaccacgg tatctatgaa
    21781 ctgccgtaga agtgaggtgt ttctatggta gaggatatgc gtgtatatat cgctaatctt
    21841 gggaaataca atgaggggta tctggtcggc gactggttct cttttccgat tgacgaagaa
    21901 gatgttgccg agcgtatcgg cttgaatgaa cggtacgaag aatacgccgt acatgatacg
    21961 gaaaactttc ccattgagat tggcgaatat atctcgattg aagagctcaa tgaaatgtat
    22021 gaaatgatat gtgagcttcc cgactacatc acggacagtt tggacgaatt tgtatcaaat
    22081 tatggaagtt tggaggaact gtacgaccat agggacgata tgattttata cgctggctgt
    22141 gaggatatga ccgacgttgc ctattacttc attgatgaat tgcaggcact tggcgatatt
    22201 ccccctagtt tgcagaacta tattgactat gaagcatacg gcagggattt atcctttgag
    22261 ggaactttta ttgaaacaag atacggtatg tgtgagatac cgtggtaaga agctggaaga
    22321 tcagcgacaa gcattgttgc ttctgacagc tttttctatt ggggacagtc tgaaaaacgg
    22381 ctgtcctttt ctattatacg aaaggagttg aaacaaaaac gtgaaaaaga ttaaaagcta
    22441 cacgggtatc tggaacgtgg aaaaagtctt gtatgcaatc aatgacttta acctgccctt
    22501 tcctgttacc tttacacaga ttacatggtt tgtgataacc gagtttatca tcattctgtt
    22561 tggggactta ccgcccttat ccatgattga gggggcattt ttaaaatact tcggtattcc
    22621 cgtagctctt acatggttca tgtcgcaaaa gacctttgac ggcaagaagc cgtacagctt
    22681 tttgaaatca cagataacct ttgccctacg accaaagatt acttatgcag gaaaagccgt
    22741 gaaactggat aagcaggtac tacatgaagc tattacggca gtaaggagtg tgagctatgt
    22801 tcccgattaa atatatcgac aacaaccttg tctggaacaa ggacaatgag gtgttcgcct
    22861 actatgagct gataccttac aattacagtt ttctatccgc tgaacagaaa ttcattgtgc
    22921 atgacagctt ccgacagcta atcgcacaat cccgtgaggg caagatacac gctttgcaga
    22981 ttgccacaga aagctcaagc cgaagcatac aagaacagtc aaagaagctg gttacgggac
    23041 gactaaaaga cgttgcctat cagaagatag acgaacagac cgaagcgtta gtatctatga
    23101 ttggggacaa tcaagtggac taccgctttt ttcttggctt caagctcatg gtaacagacc
    23161 agagcatgaa cctaaagagc gtgaagcaat cggcgtttct cacattcaag gagtttatcc
    23221 atgaagtgaa ccacacgctg atgaatgact ttgtttccat gccaaatgat gaaatcaacc
    23281 gctacatgaa aatggaaaag ctgttggaaa ataaaataag ccgacgcttc aaagtaagac
    23341 gcttagataa aaatgacttc ggctatctga ttgaacacat ctacggcagg gacggtgtcg
    23401 cctatgaaga ttatgagtat agcttaccga agaagaaact gaaaaaggaa acgctgataa
    23461 cgcattatga cttgatacgt ccgacccgtt gccttgtgga agaaagccag cgacatttac
    23521 gcttggaaca tgaggacagc gaaagctacg tttcctactt tactgtcaat gcgattgtcg
    23581 gcgagcttga ttttccgtcc agtgaaatct tctatttcca gcaacagcag tttacttttc
    23641 ccgttgatac cagcatgaat gtagaaatcg tggaaaaccg aaaagcgtta tcgacggttc
    23701 gcaacaagaa aaaagagctg aaagatttag acaatcatgc ataccagtct ggcagtgaaa
    23761 ccagctcaaa tgtggtagac gcattagaca gtgtggacga gctggaaacg gatttagacc
    23821 agtccagaga aagtatgtat aagctctctt atgttatccg tgtatctgct ccagaccttg
    23881 acgagttgaa aagacgctgt gatgaagtca aagacttcta cgacgatttg aacgtcaagc
    23941 tggtacgccc tgctggcgat atgctggggc ttcactctga atttcttcct gccagcaagc
    24001 gatacatcaa cgattatgtg cagtatgtca aaagtgattt tctagctggg ctgggctttg
    24061 gtgcgacaca gcagttaggg gaaaacacgg gtatctatat cggctattct gtcgatacgg
    24121 gacgcaatgt ttatctacaa ccgtcccttg ccagtcaagg cgtaaaagat acggttacta
    24181 atgctcttgc ttctgccttt gtagaaagtc ttggcggtgg aaaatcattc tgtaacaacc
    24241 tgcttgtcta ctactctgtg ctgtttggcg gtcaagcggt aatcttagac ccaaaatccg
    24301 agcgtggcaa ctggaaagaa acgctcccag aaatcgccca tgaaatcaat atcgtgaacc
    24361 ttaccagcga cagagaaaat gcagggcttc ttgacccgtt cgtgattatg cagaatgtga
    24421 aagacgctga aagtctggca atcgacatac tcacattcct tacgggcatt tcttcaaggg
    24481 acggcgagaa gttccccgta cttagaaagg cagtgcgtgc cgttacccag agcgaccagc
    24541 gaggattgct ccatgtgatt gaggaattaa gaaaagaaga tacacctatc tcttgcaata
    24601 ttgccgacca tatcgacagc ttcacggact acgactttgc acatctgctg ttctcggacg
    24661 gtacggtgca aaatgcaatc agcttagaca accagctcaa tatcattcaa gtagccgact
    24721 tagtgctacc cgataaggac acgacctttg aattactgtc cgtttccatg ctgattgtga
    24781 ttagtacctt tgcgttagat tttatccact cggatagaag tgttttcaag attgtagacc
    24841 ttgacgaagc atgggcgttc ttaaatgtgg cacaaggcga aacgctctct aacaagctgg
    24901 ttcgtgctgg acgtgccatg aacgcaggcg tttattttgt tacacagtcc tctggcgacg
    24961 tgtcaaagga aagtctgaaa aacaatatcg gtttgaaatt tgcctttaga agcaccgata
    25021 ttaacgaaat aaaacagacc cttgaatttt tcagcattga caaagaggac gagaacaacc
    25081 agaagcgatt gcgtgatttg gaaaacgggc aatgcttgct tcaagattta tacggacgtg
    25141 tgggcgtggt gcagatacac ccagtctttg aagaattact gcacgccttt gataccagac
    25201 cgccgataca gcgaaatgag gtggagtgat gaaagaaagg ataaaaagtg tgttcacaag
    25261 aaaaaagata ttccgctttc tcaaaatggc tctgtttgtc gtggcacttt cccttatcct
    25321 gctttcactt ttgggaacgg tggcacacgc aacgggactt gtggacgaca ctatcaacac
    25381 cgagaacctt tattccaaat atccgctgtc gaattatcag cttgatttct atgcagataa
    25441 taactgggcg tggttgccgt ggaactggct ggacgggatt ggaaaaagtg tgcaatatgg
    25501 gctttactgt attaccaact ttgtctggac gataagacta tatctaagca atgcaacggg
    25561 ctatgtagtg caggaagcct acaagctgga ctttatcaac gatatggcag acagtatcgg
    25621 aagaagcata cagacccttg caggcgtaac tcaaaatggt ttttctactt cgggctttta
    25681 tgtgggattt cttctgctga ttatcttagt cgtaggtatc tatgttgcct atacgggact
    25741 gataaaacgg gaaaccagta aggcactaca cgccgttatc aactttgttg tggtgttcgt
    25801 attatcggct tcttttatcg cctatgctcc tgactacatc aagaaagtga atgacttttc
    25861 atcagacatc agtaccgctt cactggactt gggaacaaaa atcatgcttc ccgacagcga
    25921 cagcaagggc aaggacagcg tggacttgat aagggatagc ctattttcca tacaagtaca
    25981 acagccgtgg ctactgcttc aatttggtaa ttcgggtata gaggaaatcg gggcagaccg
    26041 tgtcgaagct ctcgtatcgg caagcccaga ggacgaggac ggcaagacca gagaggaagt
    26101 cgtgaaaaca gaaatcgagg acaacgacaa caacaatctg acgatactgc aggtggtaaa
    26161 ccgtttaggt atggtgttct tcctcttgtt cttcaacttg ggtatcacga tatttgtatt
    26221 cttgcttacg ggtatgatgt tgttcagcca gatacttttt atcattttcg ctatgtttct
    26281 gcctatcagc tttctattaa gtatgatacc gagctatgag agcatggcaa aacaagccat
    26341 agtccgtgtg tttaatacga ttatgacaag ggcaggtatt acactgattg tcacggtagc
    26401 ctttagtatt tccagtatgt tctacaacat atcgacggac tacccgttct ttatggtggc
    26461 gttcttgcag atagtgtatt ttgcaggtat ctacatgaag ctgggcgatt taatgagcat
    26521 gttctcttta aacgctggcg acagtcaaag catgggacgg cgaatattcc gcagaccgta
    26581 tctgttcatg cgacataggg ctagacgtat ggaacatagg cttgcaagag ctgttagtgc
    26641 tggtggtgtc gctggtgcgg tggctggtgg tgtcgttgcc agttcaaaat ctacacgcca
    26701 gaatggaaca aagaacaatc gtggaaatac aagctccagc atggggcaga aagcaggttc
    26761 agccgtggga gctgtccttg atacaaagaa taaggtcaag gacaaggcaa ctgctgtcaa
    26821 agaaaatatc aaagatatgc cgacacagac tgcctatgcg gttcactctg ctaaggaaaa
    26881 ggcgaagtcc agtgtgtctg atttcaagcg tggcgtggtg caggaaaagg aaaaccgcca
    26941 gtctggacgc ttaaagaagc aacagctaca tagacagaat atcgctgaca agcgtatgga
    27001 acttcaaaag gcacaagaat caaggcaggc aggtaagaaa acggacggat cagcgacaag
    27061 cggagctacc cgtccccatg aaagaccagc taccacttct aaggttaatg tagaaaaaca
    27121 gcaggaagtc aaacgccctg ccatagcttc aaccgtgaag aatgatgaac ctatcaagaa
    27181 aaatgttgtc aaggaacgtc cactatcttc tggcgataca tcagcaaaga ccgtcaatca
    27241 agaaaccaca agacagaatt acaagactga ccgtgttacg aaaacaaccg ttcagaagca
    27301 tacacagcag aataccgaga agaacaaacg aaccgtattc aagaaaggac aaaagaagaa
    27361 atgaaactaa gacatatcgc tattgttggc agtctgtttt ctatcctctt ttctctcgta
    27421 ttattctttg gggttctgat tagtgccgag gacgacgacg ggggcaatgg ttattcctct
    27481 aattatgtag gtatgaacct atctgccgaa gtcttgaaac atcagcctat ggtagaaaag
    27541 tatgccaaag aaaacggtat ttccgagtat gtcaatgtgt tgctggtaat catacaagtg
    27601 gaaagtggcg gtacagccga ggacgttatg caaagctctg aaagtctggg actaccaccc
    27661 aactcactag atacggaaag ctcaatcaag caggggtgta aatattttgc gtccctgctt
    27721 tcttcctgca aaaatcaagg tatcagtgat ataaatgttg tgatacagtc ctataactat
    27781 ggcggtggct atgttggcta tgtggcagga aacggaaaaa agcatatgtt caaccttgcg
    27841 gaaaacttcg caagggataa gtcgggcggt caaaaagtaa cctatacgaa ccctatcgcc
    27901 gtggcaaaaa atggtggctg gcgttatggc tatggaaata tgttctatgt ggagcttgtg
    27961 aaccagtacc ttgttgtcaa tcaagtatcg ggcgagctgg cacaaaaggt tatgaatgaa
    28021 gccttaaaat atcaaggttg gaagtatgta tatggtggca gtaacccgaa cacttctttt
    28081 gattgttcgg gactggtaca gtggtgctac aacaaagctg gcgtatcttt accgagaacc
    28141 gcacaaaatc agtatgacgc aacccagcat atcccactat cgcaggcaaa ggcaggcgat
    28201 ttggtctttt tccacagtac ctacaatgca ggaagctatg taacccacgt cggtatctat
    28261 gtaggaaaca ctcagatgta tcatgcaggc gaccctatcg gatatgccga tttaaacaat
    28321 gcttattggc aacagtactt gatttgtgct ggaagaataa aacagtagaa aggattggaa
    28381 accatgttta aaaagaaaga aaaaccagta aaagaaacga aagtccgcac tatgaaagta
    28441 ggcacacata agaaatctgt gattgctctg tgggtggtgc ttatcgccag cgtatcattt
    28501 ggcgtgtata agaactttac cgccattgat atgcacacgg tacatgaaac ggaaaccatt
    28561 cagttaagac taaatgacac caacggagtt gaaaattttg tgaagaactt ctgtaagtcc
    28621 tactacacat gggacaacag caaggaagct atcgaagcaa ggacgcaggc aatcagcaac
    28681 tatctgacaa aggagttgca ggacttgaac cttgatacca ttaggacgga tataccgaca
    28741 agctccactg ttacaaatgt actgatttgg gatataaaac agtctggaac gaacgatttt
    28801 atcgctacct acgaagtaga tcagcagata aaagagggag aacagacaag caatgtcaaa
    28861 gcaacctata ccgtcaaagt ccatgtggat aaagacggcg atatggttat cgtacagaac
    28921 ccaaccattg caccagcgat tgaaaaatca gactatgagc caaaggcaca agaagccgac
    28981 gcaagcgtgg acgcagatac cgtcaatgac gctaccgcat ttttggaaac tttctttaag
    29041 ctgtatccga cagcaacaga aaaagaactt gcctactatg tggctggcaa tgtgcttgaa
    29101 cctatcggca gggactacct ctattccgaa ttagtcaatc ctatgtttac ggatgacagg
    29161 gataatgtga aagtcagggt tgcggtgaaa ttcattgata atcagacaaa ggcaatgcag
    29221 gtatcacagt ttgagcttgt gttacataag gatagcaact ggaaaattat tatgtaagat
    29281 attttgttgg caggctctat tttcggatat agtctgcctt ttctatagca aatgtgctgc
    29341 actaacaagc gatttcattg tattttaagc aattaaatct ttcttgacaa ttttatctta
    29401 aatcatatat ttatggatat tgactttctg cttgcaataa gttataatat gactataaaa
    29461 ctaaattagt catataggag gcgtgtggta tgtataaaga agttggtatt tcttcaaata
    29521 atgttccgat aacactttct atttggtata cagaaagaaa tagtcctacc attgtttttt
    29581 tgccgggtac tatgtcacac cctctcatgt atgagaattt tctatgtggt ttgagtgaaa
    29641 gaggatttaa tataataggc attcactatc tttcacacgg aaaaagccct aaaatcaaaa
    29701 aaaattatac aatggaagat ttgctgcata atgtatatga cgcaacgact tacggaatag
    29761 aaaattttgg tgaaaacata gctgtcatgg gttccagtca aggtgggatt ttagctgcaa
    29821 tggcggcggg tagggacaca aggttaaaag ccgtttttcc acataatatc atgctgacta
    29881 cattaaaaga aactatgagc ttaacaaaat accccgcatg gtcacaccgt tttatggggc
    29941 tgatacagtt gggctttcgt gtaggtggaa aattgctgcc taactataag gttccaggag
    30001 acgcttattt agactatgac agagtatttt atagtgaaca ggcaaaacaa gattgcttaa
    30061 atgaccctat gcttttaccc tattatcctt taagatttgt cgccagctta tttaatgctg
    30121 acttatcgtg cttggagaat ggggctatac aatgtcctgt tattttaatt actgccaaag
    30181 gcgatccgct ttttagattt gattatacca acaaagtatt tgatttaatt catgtaccga
    30241 aaaaggaatt acttacttta gagcttaatc gtcacatgat ttttaatgag gacacggaca
    30301 ctacaataaa cgcattggaa gctaccttgc ataaatatct tggatagtat attgattata
    30361 taatcagtaa atgttatact tttcatgacg ataaaaacat tttagtcata agggggttat
    30421 gagtttgcca cgatttacgg aacaagaaaa agaaatcata aacagtaaac ttttgataga
    30481 gggagaaaag ctgttttcat tatacggctt aaaaaaagta acagttgatg atttagcagc
    30541 tgctgtaaac atatcaaaag gttcattcta tgctttttat cccagtaaag aacatctata
    30601 tgtggaaatc aattttcgct tacaaaaaga gttatttaca agtatagaaa caactgttaa
    30661 aaagaaaaag tataagaacc atagagattt agcaaaggac gttattatgc taagtttaac
    30721 gggggtgatt acttcgccta ttctttcaca gattgattta tctttaatgg actatctgca
    30781 aagaaaaata tcgtcagata tttttgaaaa tcatatgtac agtgatattc gtatattgga
    30841 gatactggaa ggtttagggg ttgtattttt ggttccgcat acggttatca taaaatccct
    30901 gtattctgtt ttatcctgtt tggaacaatt caaagaggac gaagaattga atatggtaca
    30961 aaatctttta gtgaatggta ttattcagca agtagtagca gagtaataat tttgtttggt
    31021 tatttgacag gcaagtggcc aaacaaattt tttacagttt atatgactat atatctataa
    31081 acgtcataaa gttaaaggag gaaagaataa tgattgatgt aaaaaaactg tattttagtt
    31141 atactgacaa accgtttgtt gagaatgtaa gtttccatgt tgggagagga gaaattttcg
    31201 gttttttagg accctctggt gctggaaaat caactattca aaaaatttta actgggctta
    31261 atacaagtta taaaggcagc gtaaaagttg caggtacaga aataagggag catacaaacc
    31321 ggttttatga aaatattgga gtagactttg aattttcaac ctgctacgag aaattcacag
    31381 cacgacaaaa tctcgcctat tttgcttctc tatatgacaa gcaacctcgg tctattgatg
    31441 aattattgca tatggtaggc ttagaaaatg atggggacaa aaaagttgct gacttttcca
    31501 aaggtatgcg ttctcgcttg aatttcatta aagcgttagt acatgaccct gatatattat
    31561 tccttgatga acctacaagc ggtcttgacc ctacaaataa tcgtttaatg aaagatatta
    31621 ttcttgcgga aaagaaacgc ggaaaaacga ttatcattac tactcacaat atgtatgacg
    31681 caacagagct atgtgaccaa gtagccttta ttgttgccgg aaaagtcagt gcattggata
    31741 gtccacataa tttgattatg tcaaggggag ctgccaaaat acactatacc tactatgata
    31801 aaggcgagaa aaccggagaa tgtctgcttg atagaaccac agaagataag ttgttaaaaa
    31861 gtttaatttc cgaaaaccgt ctgctttcta ttcatagtag tgagcctaca ttaaatgata
    31921 tttttgttga tattacaggg aggacacttc aatgagattg ggacgtttaa tttgtggtga
    31981 tatacatttc caatggaaat atggtttcta ttttatctac tttatattaa cagttttata
    32041 tgtgtgtggg attgccgctt tgacgggaca ttggaaaacg gatattgctt ctatcatgat
    32101 atactctgac ccggcagcta tgggattgtt ttttatgggg gcgattgtcc ttttagaaaa
    32161 aagccaaaag gtattaaatg ctatggtagt atcgcctgta aaagtatcgg agtatatact
    32221 ttcaaaaacc atcgcattga ttgctatttc tacaatcatt gcggtgatat tgggatttgt
    32281 ttccggcagc aatcatttac ttggtatcgc agttggtaca gctttgacat cagcaatctt
    32341 tactatgctt ggcattatcg cggctacaaa aatttcaaac ttgaaccaat ttctcattgt
    32401 gattatgccg atagaaattg tttgctttgt tccacctatc gttggtctgt ttgtaaagtt
    32461 accagatata ttccgctttt ttccgttcac agcttgcatg aacctcataa ctggaaaaag
    32521 cattttacta tcgtttgata tggtacttgt aattgctacg ttgattatcc tctacatagt
    32581 tgcgcgacat acagttaagc atatgtggag aagtttggga ggtgtaaagc tatgaaaagg
    32641 atattatcca gtaccataca ggtatttaag caaatcaaga gcgaccctat gatgtttgca
    32701 gcttgtttta cgccttttgt catgggagct ttgattaaat tcggtattcc ctttcttgat
    32761 ggaataacag atttttcttt gcaaacatac tatccgatct ttgatttatt actttctatc
    32821 atggctcctg tattgctctg ctttgcattt gcaatgatta cattagagga aatcgacgat
    32881 aaagtgtcac ggtacttttc gattacccct cttggtaagt cggggtatct ctttacaagg
    32941 ttgggagtac cctcaattat ttcggcggtc atcgctttta ttgtactgtt gcttttttca
    33001 ttagaaaagt tgtccattgg aatgacaata tgtttggcgc ttctcggctc agtacaggca
    33061 attattgttt cgcttatgat tataacatta tcaagcaata aattagaggg tatggcagtt
    33121 acaaaactag ctgcacttac attactcgga ataccagctc ccttttttat tgatagttac
    33181 taccagttcg cggttggctt ccttccatca ttttgggtag cgaaagccat gcagaatgaa
    33241 gcggttcttt atttctccat aggattgatg gtagctttgg tctggtacta cttccttata
    33301 aaacgtctgt ttaggaagct ggcaggataa gaaggcatat tatggaaaaa ggtagacttc
    33361 tgcaaaacga aaatataaat aaggctattc aggtttccaa aatcacacta atcatcaacc
    33421 tgggtttatc gctacttaaa tttgctgccg gatatatagg aaagtcaagt gccatgctgt
    33481 cggacgctgt acactcggct tctgatgtat taagcacaat cgttgtgatg gttggaatta
    33541 aaatatcaga aaaacagcct gataaagaac atccttatgg gcatgaacgt atggaatgtg
    33601 tggcttccat tattctctct gttgcattgg ctataacggg tgctggaata ggatattcag
    33661 gtatcaagaa aatcttttca ggacagtaca atacgctttc tgtctctagt ggaatcgcac
    33721 ttacggcggc agtcttatcc attgttataa aagagtggat gtactggtat acaagaagtg
    33781 ctgcaaagca tacaaattct gacgcactta tggctgacgc atggcatcac cgctctgatg
    33841 cactctcgtc ggttggttcc ttaattggca ttttaggagc acgtttaggc tatgctattc
    33901 tcgaccctat tgcaagcatt gtgatttgcg gttgtatctt aaaagctgct ttggacattt
    33961 tcaaagaaag cattaacaaa atggtagacc atagttgtga caatgctact gaaacgaaga
    34021 ttagggaagt tgtgcttcag cagcaagggg ttgatggtat tgatgaatta aaaacccgca
    34081 tgtttggagc gaaaatgtat gtagatattg aaatattagc tgatggtaat ttagctcttt
    34141 atgacgctca tagaattgct gagggagtcc atcaaacgat tgaaaacaac tttccacaat
    34201 gtaaacattg tatggtacat gttaatacga atgaacttcc ctattctcca taggacaggg
    34261 aagatgattt aagactattg aaagggaaag gaggggaagt aggttatgga acaatactgg
    34321 tggattatca ttgttatggt tttgattgtc gttggcttac ttaaaattgt aattatgaag
    34381 aagtataacc aaaggagaaa ttctaacaat cgttcaaaaa atagcgagga tgacaagatg
    34441 tattaagagc aattatccga tggtatgaga tatataccta tgaaatttga acgataaatc
    34501 ccagtttgta aaagtcaata aatccaaaga taaatttgta tttgaaagga gttttgaaga
    34561 atgaaaaaat atgagtatgt agaaattaat tataatgcga aaggagttgt attcctttgt
    34621 actgataaac acaaagaaat tattgacagt tatgcaaaaa cggggtatca ctacgcaggg
    34681 tttgtaccca cagaaattga cgcaaaaggc tgtatgcgaa agatagattt aatttttgaa
    34741 aaagaagatt aacttatttt agggagttgg ttctaatgat aggacttaaa agacgagata
    34801 tgaattatat tatgaaagtt ggtgctaaag ctcaaggtgt ttctgtatct gaatttaggg
    34861 ctatgctaca agataccata gacaaaacta tggaaagtac agacccagac gtgcaggcaa
    34921 attttaaacg atactttgga aataagcggc caacaccaga ggaatatatt tatacgatta
    34981 ccaaaaagac aaaggttaag gtttaaatgt tgtaaagggg gcttgccgta tgaaacgctt
    35041 taaatacaat tcagtatatt caagcccaat ttaacagcat actatttaca attaaatacg
    35101 ctataccagt agctcgtaca aaatttatat tttgtgcggg cttttttaat acccgaaaag
    35161 ttttttaatt attttcattt ttttggaagc aaatcacacc ttgctgtaca gatatggtgc
    35221 ggaaagaggg ataaacactt ttcaattcat agctggaaag gaggtgagat tatgaaaccg
    35281 tctgacttcc aaaaaacaat tcagtgtcag tttgaatgta agctgaaaaa agttgtcaaa
    35341 ggtattatct ggaactaccg caaagaatta agacgaagaa aaaacaagga aatatctttt
    35401 agcgaattac cagacattac ggtagaaagt ctggcaacat gggacgacta cgaaacaaac
    35461 tacacaattt ttaatgtgtg tggcatagat attaaagtcc ttgatgatga acttgcagaa
    35521 gctctcaaac agctacccga aaaaaacgta acaatgtatt gatgtattac ttcttggaaa
    35581 tgagtgatac agagattgca gatattatga attataccag agaggctgtg ttcaaacaca
    35641 gacaccgtgc actagaaact atgaaagaga tactaaatga aaagacaaga cagtaaagga
    35701 cgcaatttgc gtcttggaga gagccagaga aaagacggaa gatacgttta taaatatacc
    35761 gatatattcg ggaagccaca gtttgtatat tcttggaagc ttgtacccac agacaagaca
    35821 cctgctggaa aacgtgatga tatatcgttg agggaaaaag aaaagcagat aaaaaaagac
    35881 cttgacgacg gtatcgacac ggtgggcggt aaaatgacag tctgccagct ttacgaaaaa
    35941 aagaacaatc aaagaaagaa catcaagaga aacacggaat taggacgaca gtatcttgtg
    36001 aacgcactgg aaaatgaccc attgggtatg agggcgattg atacggtcaa gcagtccgac
    36061 gctaaagaat gggctatcag aatgaatggc aaaggttatt cttacaagac gatagataac
    36121 tataagcgtt ccctaaaggc ttcattctat atggcgatac aagacgactg tatcagaaaa
    36181 aatcctttta attccaagtt gagtgatgtt ctggaagatg atacggaagc aaaggttatc
    36241 ctgacaccag agcaggaaga aaaactcctt gcctttatgg aaacagataa tgtttacagc
    36301 aagtatcgtg atgaagttat tcttctgctg gaaacgggac ttcgtatctc tgaattatgc
    36361 ggactgacaa cacacattga tatgcttaac cgtgtgatta acatagacca ccagctattg
    36421 cgtgatacag aggtgggcta ctatatttcc aaagctaaga caaaaaacgg taagcgagag
    36481 cttccattaa cagaaagagc atatcaagca ctcaagcgaa ttttaaagaa ccgaggaaaa
    36541 gcacaaccgc ttgttgttgg tggttatagc aatttcttat tcttgaaccg tgagggattg
    36601 ccaaaggtag caggaaatta tgaaagtatg gtgcgtgggt tgattaagaa gtacaacaag
    36661 acccataagg acaagttacc aaacatcacg ccccattcat tcagacatac ttactgtacg
    36721 aacatggcaa ataagggaat gaacccaaac accctgcaat acatcatggg acacgcaaac
    36781 ataaccatga cattagggta ttatgctcac ggtactttcc agtctgcaaa ggcagagtta
    36841 gaaaggctgg cttcttaata tcgaaaatgt atcttactac ttatttacta cttttggttg
    36901 cattttcatg ctggtaaatg ccagcttatg caaggtatct tccaaaaaga aaatgcccat
    36961 acagcccgaa aatgcgggat atatcggcat ctaccaactt atgaaaagat aatcagaaat
    37021 ttagtaagtt tttataataa aaaaataaga ggctgtccaa aaatagagat aagccttttt
    37081 tcataatcgg aattataaaa atacacttga tttcatatga aaaagagtgt ctcataaact
    37141 aaaaagttta ctttgagaca ctaccttttt tattcttatt tatactttgt agacatatta
    37201 taatatgcgt ttcactggta tctgaacttc tgtcaaccag tcttcttcac tctctttatt
    37261 ccatatacca tctatataag attctctagg atttccaacc actttatatt tattgtcctc
    37321 tatccattta aatgcatctg cataagtttt tcctaatgtt gaatatggac cattgtgtaa
    37381 tatacaaact gcattttcaa ctgcatctaa ttctttaaat tttaatatat ccgtatcttc
    37441 ctttaaatca tttatagctt gacacatttc aacatccata tcagtctctc tatattcacc
    37501 atcatgatat atattaaaac aataatcagg aactgcacat gtacaccctg ttttcatcat
    37561 ttcttcagcc attacatttg gaaatagatt aaaaagttca tcatatccct ttaacacttt
    37621 tctcatagaa gcaactttaa cttttggtaa agacttaata attacatcat tcatattgac
    37681 ttctcctttc aaattcataa ggtaattacg tagtttaatt attttatatt cttcactttt
    37741 aattacatcc aacatttctt tttctttatt cattaaaata ttttcaactt cttcaggatt
    37801 ttctacagct ttttttatct cagctaatga taaacccatt tgttttaaag ttattatctg
    37861 atgtaattta ggtaattgct ctgttgtata atacctatat tttgtaaatt catctacata
    37921 ttctggtttt aacaacccta tatcttcata ataccttaat gtctttgtag ttattttatt
    37981 tattttagaa aacattccta tagaaaacat ctcatcacct gcgcattaga tttgattaat
    38041 ttaagtttaa actttcccct taggttaaag tcaatacaaa ataaattata attgtattaa
    38101 ttcttcaatt ctatctccac tcaataccaa aatatcccct gtttttataa tggttgaacc
    38161 tttaggtatt aaaacctgtt cctttctttt tatcataact attaatatat cgtctggtat
    38221 attagaatct attatactct tattagccca tttattaccc tcatcaacat ttacttcgat
    38281 aagtttagag ctaatttccc catcataatc attaaatgtt tttaatacag gtgtattatc
    38341 atcaactaaa tccaactttc tggctacagt tggtatcaat gaaccttgta ttcctactga
    38401 aagcaacgct ataaaaaata ttatatggaa tatgtcattc tctatggcta ctccttgagt
    38461 aacagcataa attgcaaaaa ctactgaagc cgcacctcta agacctaccc acgatatgaa
    38521 taatttttct ttatttgaga atttaaattt tgctagaact ataaatgttg ctaatggtct
    38581 tgcaataact atcatgaata tagatatggc cattgctata ccaaatacat ttggtaactt
    38641 agaagggaaa gataacaatc ctaacataaa gaataacatt atttgcataa tccatgaaat
    38701 tccatcaaag aaatgaacca aacttttttt aagaggtatc ctactatttc ctataataat
    38761 acctgaaatg taaacactta aatatccatt tcctccaaga tactcgctaa atgcataaga
    38821 aagtattgca atagctgtta taaatatagt ataaaatcct tctatctcga agtttgaatg
    38881 ccttaaaata taaacagata attttgctag tataaatcct atagctaacc caaaagctat
    38941 ttgttttatt accattggtg caataagttc taccccagaa ttattcatca atgtcaaaag
    39001 aattagtgtc aacatatacg ctattgggtc attacttcca ctttcaactt ctaataatga
    39061 tgcaagtgaa ccccttaagt ttagtttttg tgaacgcaac acagcaaaca cagatgcagc
    39121 atctgttgaa gctgcaattg ccccaattaa taaaccttca agtaaactag ttcctattac
    39181 catgtggcaa aacatacctg taaccccaga cgtaattatt acacccagtg aagacattaa
    39241 tatagaaggt atagcaacag gtttagccat attccagttt gttccaaacc ctccaaaaaa
    39301 cattataaat attagtccaa tagaacataa ttgttttgtt aattcatagt tctgaacctc
    39361 ccatgactaa agtcacagag ttcctgcttc atagaatttt gcatactaaa atatgtctta
    39421 ctaattctcc acaggctatc ccc
//
LOCUS       DAEPOG010000015        37239 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEPOG010000015 DAEPOG010000000
VERSION     DAEPOG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.015"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(96..1250)
                     /gene="nagA"
                     /locus_tag="KR432_000687"
     CDS             complement(96..1250)
                     /gene="nagA"
                     /locus_tag="KR432_000687"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0639242.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1333..2058)
                     /locus_tag="KR432_000688"
     CDS             complement(1333..2058)
                     /locus_tag="KR432_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639243.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2327..3256
                     /gene="pfkB"
                     /locus_tag="KR432_000689"
     CDS             2327..3256
                     /gene="pfkB"
                     /locus_tag="KR432_000689"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0639244.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(3385..4302)
                     /locus_tag="KR432_000690"
     CDS             complement(3385..4302)
                     /locus_tag="KR432_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0639245.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            4764..5933
                     /locus_tag="KR432_000691"
     CDS             4764..5933
                     /locus_tag="KR432_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0639246.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            5982..6833
                     /gene="gatY"
                     /locus_tag="KR432_000692"
     CDS             5982..6833
                     /gene="gatY"
                     /locus_tag="KR432_000692"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0639247.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            7159..9060
                     /locus_tag="KR432_000693"
     CDS             7159..9060
                     /locus_tag="KR432_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0639248.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            9063..10106
                     /gene="ypdE"
                     /locus_tag="KR432_000694"
     CDS             9063..10106
                     /gene="ypdE"
                     /locus_tag="KR432_000694"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0639249.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            10121..10426
                     /locus_tag="KR432_000695"
     CDS             10121..10426
                     /locus_tag="KR432_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0639250.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            10523..10858
                     /locus_tag="KR432_000696"
     CDS             10523..10858
                     /locus_tag="KR432_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639251.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            10890..12212
                     /locus_tag="KR432_000697"
     CDS             10890..12212
                     /locus_tag="KR432_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0639252.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            12222..13292
                     /locus_tag="KR432_000698"
     CDS             12222..13292
                     /locus_tag="KR432_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0639253.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            13297..14484
                     /locus_tag="KR432_000699"
     CDS             13297..14484
                     /locus_tag="KR432_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0639254.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(14681..15553)
                     /locus_tag="KR432_000700"
     CDS             complement(14681..15553)
                     /locus_tag="KR432_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBH0639255.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(15947..16138)
                     /locus_tag="KR432_000701"
     CDS             complement(15947..16138)
                     /locus_tag="KR432_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639256.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            16358..17404
                     /gene="cobT"
                     /locus_tag="KR432_000702"
     CDS             16358..17404
                     /gene="cobT"
                     /locus_tag="KR432_000702"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH0639257.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            17436..17996
                     /gene="cobU"
                     /locus_tag="KR432_000703"
     CDS             17436..17996
                     /gene="cobU"
                     /locus_tag="KR432_000703"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH0639258.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            18006..18779
                     /gene="cobS"
                     /locus_tag="KR432_000704"
     CDS             18006..18779
                     /gene="cobS"
                     /locus_tag="KR432_000704"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH0639259.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            18799..19413
                     /locus_tag="KR432_000705"
     CDS             18799..19413
                     /locus_tag="KR432_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0639260.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      19480..19659
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            19789..21288
                     /locus_tag="KR432_000706"
     CDS             19789..21288
                     /locus_tag="KR432_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBH0639261.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            21327..22703
                     /locus_tag="KR432_000707"
     CDS             21327..22703
                     /locus_tag="KR432_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBH0639262.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            22704..23675
                     /locus_tag="KR432_000708"
     CDS             22704..23675
                     /locus_tag="KR432_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0639263.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            23707..24777
                     /locus_tag="KR432_000709"
     CDS             23707..24777
                     /locus_tag="KR432_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0639264.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            24779..25666
                     /locus_tag="KR432_000710"
     CDS             24779..25666
                     /locus_tag="KR432_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0639265.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            25654..26286
                     /locus_tag="KR432_000711"
     CDS             25654..26286
                     /locus_tag="KR432_000711"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBH0639266.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            26295..27521
                     /locus_tag="KR432_000712"
     CDS             26295..27521
                     /locus_tag="KR432_000712"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBH0639267.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            27518..28126
                     /locus_tag="KR432_000713"
     CDS             27518..28126
                     /locus_tag="KR432_000713"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBH0639268.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            28116..28691
                     /locus_tag="KR432_000714"
     CDS             28116..28691
                     /locus_tag="KR432_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBH0639269.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            28684..29436
                     /locus_tag="KR432_000715"
     CDS             28684..29436
                     /locus_tag="KR432_000715"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBH0639270.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            29420..30541
                     /gene="cbiG"
                     /locus_tag="KR432_000716"
     CDS             29420..30541
                     /gene="cbiG"
                     /locus_tag="KR432_000716"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBH0639271.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            30538..31263
                     /gene="cobJ"
                     /locus_tag="KR432_000717"
     CDS             30538..31263
                     /gene="cobJ"
                     /locus_tag="KR432_000717"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBH0639272.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            31260..32012
                     /gene="cobK"
                     /locus_tag="KR432_000718"
     CDS             31260..32012
                     /gene="cobK"
                     /locus_tag="KR432_000718"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBH0639273.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            32031..33509
                     /locus_tag="KR432_000719"
     CDS             32031..33509
                     /locus_tag="KR432_000719"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBH0639274.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            33539..34444
                     /gene="hemC"
                     /locus_tag="KR432_000720"
     CDS             33539..34444
                     /gene="hemC"
                     /locus_tag="KR432_000720"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH0639275.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            34434..35933
                     /gene="cobA"
                     /locus_tag="KR432_000721"
     CDS             34434..35933
                     /gene="cobA"
                     /locus_tag="KR432_000721"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH0639276.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            35950..36915
                     /gene="hemB"
                     /locus_tag="KR432_000722"
     CDS             35950..36915
                     /gene="hemB"
                     /locus_tag="KR432_000722"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH0639277.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            37068..>37239
                     /locus_tag="KR432_000723"
     CDS             37068..>37239
                     /locus_tag="KR432_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0639278.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKI"
BASE COUNT        14800 a         3940 c         6638 g        11861 t
ORIGIN      
        1 ctttttgtat gaaatcaaat gtattttcat cattttaaca atgaaaaaga gacttatctc
       61 tatttttagg aagcctccat aactttatat tgaatctatt tattaacttt gtacatacat
      121 ataccactta tataagtttc gtatatatta aagttcttat ctgttactat aaagtctgca
      181 tccatacctt gatgtatgga gccacagaca ttatcaatat tacaacttaa agcaggaaca
      241 tatgtagcca tttttattgc atcttctaaa gtggctatgt tccaatcaac tatatttctc
      301 acagccttgt ccatagttaa tacacttcca gctaaactcc cactatttaa tcttgcaaca
      361 ctatttttta catttacact aaacttacca agagtatatt ttccatcctc catagcacca
      421 gccctcatac aatctgtaat taaagctatg ttattactag acttcgcatc catcattatt
      481 ttaacagcta ttgggcttac atgatgacca tcacaaatta attcagcata agtatctttt
      541 agtaccatag cagccccaac cattccaggt tctctatgat ttaaagggct catcccatta
      601 tatgtatgta caaacacttt agcacctgaa ttaacgactt gtttaacttg ttcaaatgta
      661 gctgaactat ggccaagaga aacagagata ttatttttag ttgcatatct tgttaattct
      721 attgaacctt tacgttctgg agcaattgct atttttctta ttaaattttt cgattttctt
      781 tgccataatt ttaatatatt gatgtcaggg tctttaaaat atttttcatt ttgagcgccc
      841 ttatattttt ctgtaaaaaa tggaccttct aaataaatcc cttgtatttt tgcaccctca
      901 actcgattga agctattgct tatattttct acagaatcac ttagtgtatc tacagaatca
      961 gttaaagttg taggtaggaa agatgttact ccatatttta ataagccttt tgaaattgtg
     1021 tttattgctt caaatgtatt gtccatgaca tctgcaccta aaagaccgtg aatgtgagta
     1081 tcaactaagc caggagcaat atatttacct gaaaaatcta tgacattatc agtaatatta
     1141 ggtctttctt gttgaaattc accgaatttg ccattaatta tactaaggta acctttttct
     1201 ttgattccat gtttcataaa aaatttatct gcataaataa aacttttcaa aataacctcc
     1261 ttataattat actatcattg tggcattgac gtttataata tcattctatt ttcttgaaag
     1321 ctatttaatt cactatctta catgtcttac tctatataca aatttatcgc ttctagcaac
     1381 actcaaagta aattcaataa ctatattgtc atcattatat gtggtacgaa gtaatttcag
     1441 acaaggtgaa tctcctggta cttccaataa cttagcttcc ttatcagaga ttattccagc
     1501 tgaaaattct tcatcagcaa ctttaataat ctcgccataa tcttctctaa atatttcata
     1561 taaaggtttt tgttcgattt tttcttttgt caatcctata aattctttca taggaagata
     1621 tgttctttca agcatcatag gaaggtcatc agcaagtctt aaacgcatta tttttatcat
     1681 tttttcacca ggagttatac ctaatttttc agagaaatac tttgtacttt ctactacctc
     1741 aaatgataat acctttgtac ctggcttttt tccttgtatt ctcatataat ctgtaaagct
     1801 ataactttcc attaagtttt gagaattttg acttagtgca gcaacaaaag ttccttttcc
     1861 atgtcttttg tatatatatc ccatatgttc caactcagcc atagctagtc taactgtggt
     1921 tctacttact ccatattcat cacaaatttt tctttcagaa agcattctat cgtttggttc
     1981 catctcattt tcaatcttag ttcttaatat ctcaacaagc tggtcatata ttggtctttt
     2041 atcatttctt tttatcataa aaacatagtc ctttctaagt ggttattacc acaatttgat
     2101 tatagaatat agaattattt atgtcaatga ataaagttta atcaaaattc aataagtaag
     2161 gctattaatt tattataaaa ttatggtata ttgacaaaac aagtaaaata atttatattc
     2221 aaattggtaa taaccacttt gggtatatta tagatgaggt attttttatt ttacagatta
     2281 atttattgtt tagaaaagta aaaattaata atgaggagaa tctagtatga ttacagtaat
     2341 aacattcaac ccatctatag atagaatgta taaagtgaat aatataaaca taggtgaggt
     2401 gcaaagggta gtaagtaaga atgcaactgc tggaggtaaa ggattaaacg tagctaaggt
     2461 atgtaaaatt ttaaaagaaa atcctttagc tatgggattt ctaggtggat ttaatggaga
     2521 atttataaaa aatgaactta gaaaactaga tatacaaaat aaatttacaa aaatagagca
     2581 ggaaactaga aattgcttaa acataattga ggacaataaa gttagtacag aatttttaga
     2641 aaaaggacca atagtagaaa aaagcgattt agagagattt gaaaatgata tagaggaagt
     2701 actaaaaaat actaaaatac tagtagcttc tggtagttat tgtgagaata tgccgataga
     2761 ttattatgaa aagataggaa atatatgtag agcaaataat ataaaattta tattagatac
     2821 atcaggagaa gcattaaaaa tagctttaaa atcaaaacca tatttaataa agccaaatat
     2881 agatgaaata agacatttat taggaataaa tatagaatca agagaggaaa ttatcttgtc
     2941 aggaaaaaaa cttattgaaa tgggtgcaga gaatgtatgt atatctttag gaaaagatgg
     3001 tatggtgtat ttaaacgaat ataatgtata tgatgtaaaa gtaccagttg taaagtgtat
     3061 aaatactgta ggtagtggag atagcacagt ggctggattt agtgtaggta ttttaagggg
     3121 ttataaaata gaaaatttac ttaaatttgc aaatgcttgc ggtataagta atgctttaaa
     3181 tatggaaact ggatttgtag atttgggaga aattgataaa tatcaagatt tagttgaagt
     3241 aaaaaagtta agttaaataa atttaatggg aatataataa atttaatgtt gttgtaatag
     3301 attttaattt taatataaaa gatatagtct tatataaaaa taggttagaa aaattcattt
     3361 ttaacctatt tttataataa atatctattt tactgaattt ataattttct ctagtaatct
     3421 ttgagccttg atacactctt ctccattaat agctggtttt atattatttt ctatacaatc
     3481 aataaaatga tttgatatat cctcaaaacc cttttgtttt aatatattta cccatgcacc
     3541 actttgtttt aaagttaggt ttccaccttc ttctatttcc agtacattaa gattcttaac
     3601 tctttgaatc ttaccttttg aaagaatttc aacttgttct aactttgttc cagaatctct
     3661 atgcatagag gtgtttacag agaaatgttt acctttaagt ttatgactta caaatataag
     3721 attcttattg tctgttaaaa ataaatcttc tccgctaatt tcaacatctt catttgctaa
     3781 ccataaagca gtgtcaataa catgtatata gtcatctata agggtactgt cgaatctaac
     3841 atttcttaag gaatttaatc catgtttaca tatattgata gatacgattt ctgtagcatt
     3901 atttttaatt tctttgtaca taggacagaa tctacggttg aatccaacca ttaaatttaa
     3961 gttttttttc ttacttaact cgataagttc ttctccttga tttactgttg aagcaagagg
     4021 tttgtctaca tatacatgaa cacctaaatt taataatatt tttataattt cgtagtgtgt
     4081 ttctgtagag ctatgtagaa aaatacaatc acatttttta gctagggatt caatagaatc
     4141 gaatggcata attctataat cactacagat tttttccctt ttaactttat ttggagtaaa
     4201 agctcctaca aactcaaatc tctcagactt agttaatata ggtaaatatg ccttttgagc
     4261 tatggagcca agacctatca ttcccatttt tatatttttc ataatacttt ctcctaaggt
     4321 ttttatattt acagaattga ttataattca gacacataaa tttgtaaact ttgtatcaaa
     4381 aaataaattt aaagcttata taagtaaatt aacgaaataa gaaatattga caaagtaatt
     4441 ttgtaggata tctataaaat tgattgatat aaatatttta tgtattgaca aaaaattcaa
     4501 attaatttat atttggatta gtggtaatta ccactttggt ctgaacaagt ttttatcgta
     4561 aaataaaaat actaaagtat gattttatta aatttttaaa gataaactat aaaattaatt
     4621 aaaatctata gcgtatattt tatagtgagt ttaagtagta ctaaaaaatc taagcaaaaa
     4681 attataaaag agtatatttt tttatagttt aaagtggtta taacctcatt accaaatgta
     4741 aacgttacaa gtatgaaagg agaatgaaaa tgtttaaatt agaagaacaa aaactaaaag
     4801 atttaggtgc aataataaca actaatgaaa taaaacaaca acctgagtta tggttagaga
     4861 cttatgaaat atataaatct aacaaagaaa aattaaatag attcatagat acaataagta
     4921 ataatcatgg gcagtttaga gttatattta caggtgctgg aacttcagct tacataggaa
     4981 attcaatact tccttacctt aagaataaaa atgacataag aaaatacata tttgaagcaa
     5041 tacctactac ggatatagtt tcaaacccat atgattactt aaaaaaagat ataccaacat
     5101 tattaatttc atttgcaaga agtggaaata gtcctgaaag tttggctgct ttaaatttag
     5161 ggaataagat tgtagataat ttttatcatt tagctataac ttgtaatccg caaggagaac
     5221 tagctaaaat gactaaaaac gacgaaaata attacttgtt attaatgcca tcaaaatcaa
     5281 atgatgaagg ctttgctatg acaggaagtt tttcgtgtat gatgttatct gctatgttaa
     5341 tattcgatag tttagaagat gatgtagaaa agtcatatat aaatgcaata attgaaatgg
     5401 gaagaaatgt tatagataga aaagatgaaa tacatgagtt aataaataaa gatttcgata
     5461 gagttgtata tttaggttca ggaggattag gtggtttgac acaagaagca caactaaaac
     5521 ttttagaact tacagcagga aaaatatcta cggtgtatga ttctcctatg gggtttagac
     5581 acggaccaaa atcatttatt gatgaaaata cacttgtatt tgaatttgta tctaactgtt
     5641 tatatacaag aaaatatgat ttagatgttt tagaggagat aaaaagggat aaaattgcta
     5701 aattcacttg tgcagtgtca gtagaaaatg aaaataactt ctcaggaaca aagtttgagt
     5761 ttaaagaaaa gtataataaa ttaccagatg tgtatcttgc aatgccatac atattgtttg
     5821 cacaaactat agcattgttt gtatcggtaa aggttggaaa taagccagat acgccatcag
     5881 ctacaggaac agtaaataga gtagtaaaag gtgtaacaat atatgaatat taaaagataa
     5941 gattaaaata ttataactaa ataatttggg agggtatcaa tgtggcatta gtaacaacaa
     6001 catcaatgct taaaaaggca caagatgagg ggtatgcagt aggagcattt aatgtagaaa
     6061 atatggaaat ggtaattgct gttatagaag cagctgagga gttaaactct ccagtaatat
     6121 tacaaactac tccatctact gtaaaatatg caggtcttga ttattatcta gcaaatgcaa
     6181 aggttgctgc agaaagagta aagatacctg tagcaatgca tctggaccat ggttcaagtt
     6241 ttgaccttgc aatgaaagct ttaagaagtg gatatacatc aataatgata gatggttctc
     6301 atgaaagctt tgaaaataat atatctatat ctaaatcagt agtaaaagca tgtagtcctt
     6361 ctcttatacc agtggaagca gaacttggga aagttggtgg aaaagaagat gatttagatg
     6421 gcgggaatag taatgattat acaaatccaa aagaggcaaa agagtttgtc gatagaacag
     6481 gaatatcttc attggctatt gctataggta ctgcacatgg attgtataag ggtgaaccta
     6541 aaatagattt agataggtta tcagaaatta aaaatgtagt ttcaatacct ttagtattgc
     6601 atggaggttc aggaatacat gatgatatta taaaagaagc tataaaaaga ggtattgcaa
     6661 aagttaacta tgcaactgaa ttaagaattg catatagtaa aggggttaaa aaagttttag
     6721 atgaagattc tgaggttata gaccctaaaa agtatggatt agcagggctt caatcagtga
     6781 aggattttgt aaaagaaaag attaaagtgt gtgggtctgt aaatagagta taaaaataaa
     6841 atatcctaaa tttattttag ataagtttag gatatttgtt atatgaaaaa tttatattaa
     6901 ttatatgtaa atggtgtatt tttttcattt aattgatgaa aaatatcagc ttttattggt
     6961 aaagtattac atatataatt aaaatataat aaaaattgaa ctatatattt tattattata
     7021 aaatatgtat attaaatttt ggaatcaaaa tatactagga tataaaattc ttaaataaaa
     7081 agaaaaggtt gtttttataa aaaatatttt ttataccact ttaaaaaaca caataataac
     7141 actgagggag gtggggatat gagagaacgc attattcttg aaacattaaa aaatagtagt
     7201 ggttatctaa acatagatta ttttgcagat aagttggggg tatctactcg aactatcaga
     7261 aatgaaataa aaaagataga aactataaaa gaatgtaatg gttttaaatt agaatataag
     7321 tctaaattag gatatattct aaatattaaa gaccaagata aattcgagca ttatctaaga
     7381 aacttaccct cagatttagt tgaaaatcca gaacaacggt tggagtctat aatagtagag
     7441 cttttagtaa atgaaggtta taaaactata gaacagttaa gtaaaaaatt tctagtaagt
     7501 tcatcccaaa taaaaaatga tttaaaaaag atagatgaaa agataaagga tacagaatta
     7561 aaactagagc gaaaggctca ttatggaatt aaaatagaag gttctattaa agaaattcaa
     7621 agtatattgg tggatagtta ttttagaggg aatagaaata taacagagta caggaataag
     7681 tttatagata atttaaaatt agctaatatt agaagtacaa taaaaaatgt acttaatgaa
     7741 catgatttag aagcaaattt aacagaactt gaggaaatat tagcacaaat tattatattg
     7801 tatataagag ttagtatgag agtagtggga aatgctagca atttaaaatt atatacagaa
     7861 gatttaatta tagataaatt attagataaa atttttagag ataaaaaata taaccttaat
     7921 tatgatgaaa agtattattt aaaggagctt ataaaactaa agactaaaga taagaaagcc
     7981 actataaaga atattgataa gaataaactt caagatatta tgttcgaatt ttttagagag
     8041 atagataaaa aatataactc taatttttta gaagataaag agttttttaa cttattttac
     8101 ttacatattg catgtttaat agaacgtata aagaagaacc acaagataat aaatcctttt
     8161 tcagttaaaa taagtcaaca gtatccaact gtttttaatt tatctattca attttcaaaa
     8221 atcatagaaa atgagtatca tataaaaata agtcaagatg aaatagggtt tatagctact
     8281 cacattgctg ttccttttga aaagagagaa gaagctaatt ttaataaaaa atataaaata
     8341 gcaataatat gctcttctgg tggaggtagt gcatttttga taaatttaag acttaaagaa
     8401 atttttccaa atgctgagat aagaaatttt tcattactag aagaaaaagc tgtaatagaa
     8461 tttgcaccag atttgatttt ttcaataaca aatcttttat ttgaaataaa tgctccagtt
     8521 atacttataa atgaaatttt agacgaattg gattatttag atattaaaga aagtgttaga
     8581 tttgcagacc atataggaaa tatatcaagc ccaaaacaat atatattagg tctatttgat
     8641 aagaatcact ttaggtgtat aaaagataaa gctaagtata aggatatcct tgatagtatg
     8701 tcaaaatgta tagttgacga gggagcttgt tctccaacct atcccaaaga tgtatgggaa
     8761 agagaaagct atttgagtac tatttataca aatggggtgg ctatacctca tccaatcgag
     8821 atgacaggaa acaaaaatat aatatcagta gctttgatac aaactgatat tatttatgaa
     8881 aatagggtgc ccaaaattat tttcatgatt tcattaataa agggaaattt agaacttcat
     8941 aagcaaatat ctaaatattt aagtaagatt atggcaaata aggatatggt agacatgcta
     9001 aataagtcac aatcttatga agagtttatg tataagttaa aaatctatct gggaggttag
     9061 acatgaatat agagtttttt aagaggttgt cagaagcaga tggtatagca tctaatgaag
     9121 aagaggttag acatgtttta ttggaagaac taaaggctta tagtgataaa attatctgtg
     9181 atgggctagg gagtatcatc ttttctaaaa taaaagatga gagtgcacca aacgtaatga
     9241 tttgtgctca tatggatgaa gttggattta tggtgagaag tatagacaaa cttggtatga
     9301 ttcacttaat aacaatagga ggagtaaaac ctcttgcaca gtttgtacaa aaagttagaa
     9361 ttacaacaaa agaaggaaag aaaatccctg ctgtcataaa tgctacatat aataatggta
     9421 aggctgaaaa tatatatgca gatattggag catatacaga agaagatgta tataacctag
     9481 ggattaatgt tggggatatg gtaacatata caacaagttt tgaggaattt actttaccag
     9541 accgattagt gggaaaggct tttgatgata ggataggatg ttttgtgatg ggagaagttt
     9601 taaaagaact taggaaagaa aatctaaatt gtaatattca ctttgcggct acaagtagtg
     9661 aagaggttgg aataagggga gcaaagacat caacacagtt aataaatcca gatatagtat
     9721 ttgtaataga tgtagcatgt gctaaaaatg agtttgtgag agactataca aatcagaaac
     9781 aaattgataa aggtgtaatg ctaatgcata gagataggac tctagtccct agtagaaaaa
     9841 tgatagatta tgtaatggaa attgctgata aaaataatat accacttcaa catgatatgt
     9901 ttgaatcagg tgggactgat ggaggagaag cacatctcgt aaatgaaggg aaaccttgtg
     9961 tagtaacttg tgtaccagtt agatatggtc attgtgcatt ttcaatagta agtaataaag
    10021 atttagagaa cattataaga ttgtatacac agttaatttt aaattttgat gaagaacaat
    10081 ataaacatat aagaaatttt atctaaagga gaataagaaa atgtcaacag aagtacaaat
    10141 aatggagctt atatcaactg caggagagag taaatcgaaa gcttttgagg ctttaaaaaa
    10201 agtaaagtca aaagatttta aaggagcaaa attgttaata gaggaggctc gtaaaataga
    10261 tattgaggct cacagaatac agacagaatt aataactcaa gatttaaatg aagatgttga
    10321 aagcccaaca atgagtcttt taatggtaca tgctcaagac cactatatga cttctcagct
    10381 agcaaaagac ttgatagagg ctttgatata ttgtctcgat aagtaattgt aatgtagaaa
    10441 tagattattt atgtaaaaat tcttttaaaa gtttagatgc ttttaagaga taataaaaat
    10501 acaaacttta ggaggaatta ttatgaaaat attattatgt tgtgcaggtg gattttctac
    10561 taatatgtta atgcaaaata tgaaaaaggt ggtaaaagag agtgctaaac tcaatgaaaa
    10621 agattttgag tttacagcta taccagcaga ttcaatagaa agtgaagttg atgattggga
    10681 tatagtactt ataggtcctc aaatagctca taaaatagac ttcttaaaag gaatattaga
    10741 gccaaaaaat aagccttttg cagtaataga taaagatgta tatggaaaaa tggatggagc
    10801 tacagttatg aagttggcat taataactta taagaaacat caattagctc aaggataata
    10861 aaaataatga gggaaaaggg agattagata tgttttcaaa atttgaagca tttatgaata
    10921 gagtatttat gcctttagct cataaggttg acaatcaacg tcaccttggt tctataaagg
    10981 ctggtatggt tgctatgaca ccattcacaa tattaggaag tatatttgca atacttccag
    11041 cacttccaaa tatgcttgga gaaaataatc caataagcaa attcatactt aacaattcag
    11101 caatattgga tttaccagta agtctttcca taggtttaat aggattatat gcttgtatgt
    11161 gtattgcttt taatctgggg aactactata aattatatat accaggttgt gttgcactat
    11221 caactttttc ttttttattc ctagtagcct catttacacc agatggagct ttagaaatag
    11281 gaaatttagg agctaaaggg ctatttacag gtatgatagc agggcttggt tctgtggagt
    11341 tatataattt ttgtaataag aagaatatca caataaaaat gccagaaaat gtacctgatt
    11401 ttgtatctag gtcttttgaa ttaataccta ctactataat agtttgtgga acttttatag
    11461 ttgctcgttt tatttcatta aaagtatttg gagaattacc tccacagata cttactcaat
    11521 ttttatctcc attagtagga agtatggata acccatggtc agttcttgca cttaactttg
    11581 ctatatgtac tatattcttt tttggaatac attcatcagt atttagtcct ataactagac
    11641 ctataatgat tacatttata gctgaaaaca tagcagctat gcaagctgga gaaacaatac
    11701 ctcatttctt tacagcagga gcttcaagtg cattctttgg atttacagga tgtggaatta
    11761 gtataggttg tgtaattgca tgtatgttat caaaaaatgc ttcctataag aagattggac
    11821 gtgtatcatt attccctgca ttatttggga taaatgaacc aatattattt ggagcaccga
    11881 taatattaaa tccaataatg tttataccac atgtatttgg tggagctata ataggtacac
    11941 taccaatgtt ttttatgcac tggggatggt tagcaaagcc agtatttgac cctccatatg
    12001 taggggtatt cttagaagga tttttaacta atggagattg gcattctata ttagcaaatg
    12061 cattgcaatt agtattagca gttttattat attggccatt ctttaaaata atggaacgtc
    12121 aacaaaataa taaaaaacaa aaagaagaaa ctaagaatat attctctaaa gaagaagaag
    12181 acttattgga tgatttagga ttggatttct aatattttgt tatgaataat acaatagaga
    12241 gattagtatc cttaatctat aaaagaggtg ttgacgctct tttgattaaa gcaaaaaata
    12301 ctaaaagata tataggagct ttatcaggta gtggtgtata tgtgcttgtt actaaggata
    12361 aaaattatca aattctagat ggcagatata ttgatgaagc agataaaaaa acgactggtt
    12421 tcataaaaag gattgtatct caaggttctt atataccaac tataattgag ttattaaaag
    12481 aattaaatat aaaaaagtta gcaatagaaa accaagctat gagtatacaa gagtatcttt
    12541 cactaaaaaa tgaaggtttt gaaatcaatt taataacaaa tgaattatgg aagctaaggg
    12601 ctataaaatc taaagaagaa attgaactta ttaaaaaggc ttgtgagatt acagatgaag
    12661 tatttgatga ggttatttct gaaattaaaa aagatatgac tgagctagaa gtatcagctc
    12721 taattcagta tcatgcatta aaaaaaggtg caagtggaat gtcttttgaa acaatagtgg
    12781 tatcaggaga aagaggtgca atgccacatg gaagacctac aaacaaaaaa ttgaaagtga
    12841 atgaagctat aacaatagat tttggtgttg tttatcaagg gtatcaatca gacatgactc
    12901 gtacaatatc tataggtaaa cctcctaaaa ttattaaaga gatatatgat gtagtattgg
    12961 aagcacaatt atctgcaata gagtcaataa aggaaggaac tagggcaagt gatgttgaca
    13021 aagtggctag aaaaattata gataaacatg gatttggcga atattttaat catggactgg
    13081 gtcatggtat aggtcttgga gatggtgaag ttcccacttt aaatccaaat agtgaagata
    13141 tattagtgga aggaatggta atgtcttgtg agccaggtat ttatattcca aatgtagggg
    13201 gagttagaat agaagatgat attgttataa tagatggaaa aggtgttcca ctcaataaga
    13261 catccaaaga gtttataata ttaggagagt aaaattatga aatataattt taataaagta
    13321 ttaaatcgta aagaaggaaa ttgtagaaaa tggagcaacc atgtgattaa agaaaaattc
    13381 ggcttaaatg aagatgcaat tcctatggat ttagctgata tcgattttga gtgtgctcct
    13441 gctataaaag aatctatctt aaaaagagca tcagtaggag attatagtta tacttttata
    13501 ggagatgact tctatgatgc tattataaat tggaataaaa gaagatttga tgtatatata
    13561 gaaaaggatt ggataaaatt aacttttggt actgtttcaa cccttcatta tatagttcaa
    13621 gcatatacta aagaaggaga gggtgtattg ataaatacac ctgcttatga cccttttgca
    13681 gaggctgtag caaataataa tagaaagttg tgttgtagct cattaaaatt agataacaat
    13741 agatattatt tagatttaga ggatatggaa aatcaaatga aatacgagaa tataagatta
    13801 cttatattct gtagtccaca aaatccatca ggtcgtatat ggacaaaaga agaactatat
    13861 caagtgtcag agttatgttt aaagtataat gtaattttgg taagtgatga aattcataga
    13921 gatataatat ttaaagatta tagatttgta tctttgtgga atgctcatcc agaaatttat
    13981 aaaaatagta taatctgcgt atctcctaat aaaggcttca acttaggtgg attaaaaaca
    14041 tcatatgtat taataagaga tgtaaaaatt agggagactt tattagaaag gctaaaaagt
    14101 aattcaataa cttctccaaa tgtatttgca ataccagcaa tagttgcagc atacaatgaa
    14161 agcgaagaat ggctagatgc tatgactagt tatgtagagg agaattttga aatagtttat
    14221 gatttctttg aaactaatat accaaaagcc aaagtgatga agtcagactc ttctttttta
    14281 gcctggatag atgtaagaga agtatttaga aatgaagaag aatctaaaga attttttaga
    14341 catgcaaact taactatggt agttggaagt tattttgtga aagatggaga tggatttatt
    14401 cgtattaatg tggggtgtcc tagagaaaca ttaaatgagg ctctaaatag aatcaaaaaa
    14461 acgtatataa gtatgtattg ttaaatcata aaaaaagtat ctatggttta tataaaaaca
    14521 cattatttag ttatcaaata ttactggaat tttgtattcc agtaatattt tttgattaag
    14581 gaatattatt gaggaatatt aaataatatt atttcatacc cttatattaa cttatataaa
    14641 aatattttat tattttggga acgggttagc taacctttat ctagtgtata tttatattgt
    14701 aagaagcagt aaaaactttt cccttactaa gtttattaat aaacttctta tctttataaa
    14761 ttttgttaga atttatacta tcagcaagac cataccaagg ttctatacat aaaaaaggag
    14821 cagtagaatt agtttcacta taataaggag tccaaatacc aactagaggg aaatcttcca
    14881 tagatatagt tacatattta ctaccatcat tattatatat agacacttca tctatgttgg
    14941 tgtatatcaa agcatcattc ttgaatactt ctggatttaa atgtaagttt tttaattttc
    15001 caataggtat tatatcatct gtaaaagaac catttaaatt gattttttct acatcatttt
    15061 tacttttaaa ctcaaggtag tgtttagaaa aatcattttg tttgtaaaaa ggaaggttga
    15121 atgcaggatg tccacctata gaaaagaata tatctttaga gtcagtattt tttacaatcc
    15181 attctacctc aatactatta tcatttaatg tatagtttat gattagctca aaagaatatg
    15241 gatacttctt taaagtggat tcattatctg ttaatttata agatacaaat gtatctcctt
    15301 tatctacaat ctcaaagtcc atatctctag caaacccatg ttgagtcatg ctatataaat
    15361 tttcttctat tattgtttca ttatcaaaaa gtttacctac tatagggaac aatataggtg
    15421 attgtctacc ccaatatttt ttattaccat cccaaagaat gtttttgtcg tattttttag
    15481 aaaatatttt agttagttca gcaccaaaac tgcttgattc gataattaaa ttttcatttt
    15541 gtaatatatt catttaaaat ctcctttatt atattttatg agtatattat actataaagt
    15601 ataaagatta ttaaatttta tttgatatta tatttgaaag gtagatttta gtatattttg
    15661 aaaattttgt tttgaaaatt tgggattatt taagaaattt tgtattataa atagaaggaa
    15721 atagataaat tgtatagaat aaaatatatt aagataagtt aataatgtta aatgggaagt
    15781 gagtttatgg aaaaaccaaa tacaaaagtt gttaactttc aagagtataa agacagaaaa
    15841 aaccaaagag aagtaaatag agacgaactt ttaaagctta taaaaaaaat tgtgacttca
    15901 aaatagggag ttgttaaact ccctatttta atgggtttca taataattaa gacattctga
    15961 gtccatctat ataatctttg aattgtgaga aatctgattc actacttaat tctatggttt
    16021 ctgaaccttc cgtataaaaa ttcttaagat agatagtata attattatca acacaatcat
    16081 gataaataca tctatatcca tcttcataca aactttttaa cttagaaaaa tcatccacaa
    16141 tatcatctcc ttaaagttct tttaaatata tttttacaaa aagcatctaa ttgtatgcaa
    16201 attgacaatg taaaataaag gaaatgaaca taaaatgtag aataattttt atatgtatgt
    16261 aaaaataaaa agaaaaagtt gaaattttta gaaaataaga gtatttttta tctaaaaaga
    16321 gagtttaaaa aataaaaaaa tgttgggagg ataaaaaatg agtttattag aaagtatatc
    16381 aaaaaacata tattccctag ataactcttc tattgaaaaa acaaagcaga gattggatag
    16441 gcttatacat ccaactggaa gcttaggaaa gatagaagat atttgcatgc aattggcagg
    16501 aatatttggc aatgaaaatt ttgacacaag taaaaaggtt ataattgctt ttgcgggaga
    16561 ccatggtgta tatgaagaag gcgtagcacc tgaccctcaa aatataacaa aattacagtt
    16621 tccaaatttt tcgaaaggtc tatgtggagt tggagtaata agtaaatttg taggggcaga
    16681 tgttgtagct gtagatgtag gaataaactg tgatgaaaaa ctggatgggg tattagacta
    16741 caagataaga aaagggacat caaatatggc aaaaggtcca gctatgagta aacaagaagc
    16801 cattagatgt ttagagatag gtattgaaat agcagagcag tgtatagaaa aagattataa
    16861 ggttattggt attggggaga tgggtatagc aaatactact ccaagtacag caataatatc
    16921 tgtaatttca gggtgtgacc cgttagaagt tactggtata ggtgcaggtc ttaagaaaga
    16981 aagactaaag cataaggctg aagtaattag aaaggctatt gaaattaata atccaaatcc
    17041 aactgatgga gtagatattt tatctaaagt aggaggattt gaaataggct caatggcagg
    17101 tgttatatta ggttgtagtg ctaataggat tccagtagta attgatgggt ttatttctta
    17161 tgcagcagca ttaattgcat ataagataaa tccaaaaact agagaatata tgatagcatc
    17221 tcatttatca gcagaatcag gaactaaaag agcattagac atattaaaat tagaccctct
    17281 attaaatatg gatatgagat taggtgaggg aagtggagct gctttagcat ttgatataat
    17341 agaagcttca aactatactt acaaaaatat ggcaactttt gatgaaattg acatgggaag
    17401 ataaatataa aaatttaata tgtaggagtg ttttgatgag taaaattatt ctagtaacag
    17461 gaggagctag gtcagggaag agtaactttg cagaaaaact atgttttgac agaaataata
    17521 atacagctta tatagcaacc tctattccat ttgatgatga aatgaaagat agagtaaaaa
    17581 aacatcaaga gaatagacct agaaactgga atacttatga aatatatgaa gatatatatt
    17641 ccataattaa ggagatatat aaagaacatg aaactgttat actagattgt gtaacattat
    17701 tagtaaataa tctaatgttt gaacacaatc taaatataga agagtctagt caagaagaaa
    17761 taaacaaatt agaacaatac ataaaagaac aagttgtcaa acttattgaa gaaatagaaa
    17821 aaacaaatct atactttgta tttgtaacga atgagttagg tatgggaatt gttcctggga
    17881 ataaacttag tagaatttat gctgatattg taggtcgtat aaatcaatat atagcttcga
    17941 aaagtaatga agtttatttt gtagttagtg gcatagctat gaagataaag gagtagtatg
    18001 caattatgaa aagatttatt ttaatactac agtttttgac aaggatacct ataaaattaa
    18061 atgtaggatt tgatgatgaa ttttataagt caatagtata ttttccactt gtaggttttg
    18121 tcataggtat attatcctat ttaataggat ggatttctat gcttttattt gaaccattta
    18181 tagcttctat aataataact ctagctggtg tcttaattac tggaggactt catatagatg
    18241 gattaggaga tacatttgat gcaatatata gttataggga taaagaaaaa atgcttgaaa
    18301 taatgaaaga ttcaagatta ggtacaaatt cacttctagc aattatgttt gtacttttat
    18361 taaaggttgg attcgtttac gatataataa gtaataattc attgtggatt ataattttta
    18421 tgccaatgat tgcaaggtta ggagtaatgt tattgacata taaaaccgtg acaccaagag
    18481 aaaaaggaat gggaaattta tttataggaa aattaactac aagtatgtta ataactgcta
    18541 taatatacac attattgata gttgctctta ttactaaatt tatattttta ttacctaata
    18601 tagtgttgat taaagtatta ggttcgatta tagtggtttt tgtatttata atattattca
    18661 aaaaacatat ttataaaaaa attgacggag taacaggaga tatattaggc tgtggaatag
    18721 aactttcaga gcttgtatat ttaatttaca tatatttgtt aatttttatg tttttttaaa
    18781 gtaaaaagtg gtgataatat gataagatta atattaataa gacatgcact gacaaatgat
    18841 aataaaaaag gaagattatc aggccatata aatagttgta ttagtgaaga gggcaaacta
    18901 caaataaata aaattaccag atatctgagt aatgaaaaca tagataaggt ttatacaaca
    18961 ccatctacac gtactaaaga tacagtagaa aaaatatcaa aattgaaatt attagaaata
    19021 gaagaaaaag aagctttaag agaaattagt tttggagact ttgaaggaag aacttttgaa
    19081 gaaattaaaa tcaaatatcc aaatgagttt gaaaaaatga taaaagaagg taacaactat
    19141 agatatccaa atggagaaag tttgatagac tcttataaaa gagtggcaaa agaaattgat
    19201 aatataattt tagaaaatga tagtaattta gatacaaaga caatacttat atgttctcat
    19261 gcaggaacaa taagaaatat aataacacat ttgatttctg gtagttataa gtatcactgg
    19321 aactttaaaa ttgataatgc atctattaca gttttagaaa ttgatggtgg atttgctgta
    19381 atagataaaa tgaattttac agacttcata taatttaatc aaaaccaaac attacaatct
    19441 gtgtttgtaa tatatctgta aaattagcaa tgaaaataga atattagatt aaaattaggt
    19501 tcttaattag atgttaaaag ggaaaaaggt tagatgcctt tgcagccccc gctactgtga
    19561 aaccaacgaa actataaata ccactgtcag tttgatggga aggttataga agtaggatga
    19621 agttaagcca ggatacctgc ctaatttaat ttaaaacaag aggtcttcgg ggtgaaagat
    19681 ttttaattaa tatatataaa agtcctatct ttagaagtat taggactttt tttaatttgt
    19741 tttttagtaa attttaacaa ggagggatgg aaagtgggga aaaaaataat gctacaagga
    19801 acagcttcaa atgtaggtaa aagtgtctta gtagcaggtt tgtgtagaat atttaagcaa
    19861 gatggttata ctgttgctcc ttttaagtca caaaatatgg ctttaaattc ttttataact
    19921 aaagaaggat tagaaatggg aagagcacaa gtatttcaag cagaggctgc gaaaatagag
    19981 ccaatagctg atatgaatcc agttttatta aaaccttctg ggaataataa aagtcaagtt
    20041 attgtaagag gtaaagttgt aggagaaatg ccttcttcta agtatcatga ttataaattg
    20101 gagttagtag gtgtgttgaa ggaaacattt aatagcttaa attcaaggta tgatattgta
    20161 gttatggaag gggcaggaag taatgcagag ataaacttaa aagatagaga tatctctaat
    20221 atgggtatgg cagaaatagc tgatgcacct gtaataatag tcggagatat tgatagagga
    20281 ggcgtatttg cctcattagc aggaacaatg cttttattaa atgaagatga gaaaagaaga
    20341 gtaaaaggtg taataatcaa taaattcaga ggaaaaaaag agcttttaca agatggtgta
    20401 aagatgctag aagacatcat aaaagtacct gttataggag tagtacctta tacagatata
    20461 aaaatagaag atgaagacag tgtaactacg agatttaaga aaaaaatgaa taaaagtgat
    20521 atacacatag aaataattag aaccccacat atgtcaaatt ttacagattt taatatattt
    20581 gagacacaag aagatgtaag tattagatat gtagactatg gagaatcttt aggaaatcca
    20641 gatatattaa taatacctgg aactaaaagt actatagacg atttaacata tataagaaaa
    20701 agtggtcttg aatcgcaaat caaaaattta catagtcaag gtaaattgat atttgggata
    20761 tgtggtggat accaaatgtt aggtaaaaaa ttaaaagacc cttatcatgt tgaaagtgaa
    20821 attgaagaag ttgatggaat aggtctttta gatacagaaa caacgtttga gaaagaaaag
    20881 actacaacac aagtagaggc tactattgtt gaatgtatca gtgaatacat gaataacctt
    20941 aagggaaaaa aagtaaaagg ttatgaaatt catatgggca ttacaaagcg aggtaatgat
    21001 gtaagaagtt tagatttaat aactaaaaaa cttgataaag aagtgaatta tactgaagga
    21061 agtgtaaata aagaggggaa tgtaattggg acttatcttc atggaatatt tgatgaaata
    21121 agctttacaa gagcaatttt aaacaatata agaaagaaaa aaggtttagg agaattagaa
    21181 agcaaagtaa actcttttaa tgaatttaag gataaagagt atgataagtt ggctgatttt
    21241 ttaagagaac atttagatat agagaaaata tatgagatta tgaaataatg atgaccttta
    21301 tttataattt aggtaggagg tgtattatga aaaaaatttt aattgctggt actaatagtg
    21361 gagtaggtaa aacaacaata tcattaggaa taatgcaagc tttggttaag agaaatatga
    21421 aagtacagcc atataaagta gggccagact atatagaccc ttcatatcac acttttataa
    21481 ctggaagaca ttctagaaat ttagattctt atatgttaga tgatgaaaaa ataaagtata
    21541 tagtaaataa gtcctcaaaa gatgcagata tttcagtaat agaaggtgtt atgggcttat
    21601 atgatggttt tggaatagat ttagataatt gtacaagttc acatacttca aaggtattaa
    21661 agtcacctgt tatattagtt ataaatggta aatctatggc agcatctagt gctgctatgg
    21721 tacttggata taaggaatta gataaagatg taaatataaa gggtgttata gtaaataatg
    21781 taaagactaa tagccattat caaataatta agagttctat agaaaaatat tgtaatgttg
    21841 aagtattggg gtattttcct ccaaataaca aattctcttt agaatctcga catctaggat
    21901 tagttcctag tgtagaaatg aaatcactaa gggaaaaatt ttatacctta gcagatgaaa
    21961 ttgaacacta tatagatatt gacagaataa ttgaaatatc agaaagcgat gaatttgaga
    22021 gtgattttga ttttcaaact ttgatagaaa ataataaaat aaaagaacat gttaataata
    22081 aatcaatagc tatagcatat gataaagcat tcaattttta ttatagagaa aatatagagc
    22141 ttttagagga acttggttta gaaataaact attttagccc tcttgaagat gcatcagtgc
    22201 caaatgcaga ttatatttat attggaggag gttttccaga aatatttgga aaagaattag
    22261 aagcaaatga gtcaatgaga acttccatat tagaagcaca taacaataat atacctattt
    22321 atgcagaatg tggaggactg atgtacctag gtgagaaact attgaatcaa gaagaacaag
    22381 aatttaatat ggttggaata tttagtggaa ttagtaagat gacatcttct ttaaagagat
    22441 ttgggtactg tattggtgta gccaaggaaa atactctttt atctaaaaaa ggggaatcta
    22501 ttaaagggca tgagtttcat cattcaatat ttgaaagtga tgaaaaatgt gtatattcaa
    22561 tgagaaaaga aagagatgga aatatagtag aagagtggga tggtggttat agtaaaggaa
    22621 acactttggc tacatatctt catactcatt tttataataa cttaaattgt atagaaaatt
    22681 ttttgaaaaa aggaaatgaa taaatgagta ttttatctat ttatatagga tatgtgacag
    22741 acttaattgt aggagaccca tattcttttc ctcatccagt gagatttatt ggtaaactta
    22801 taaattttac tcaaggtata ataagaaagg tctctaaaaa tgataggcag ttaaaatttg
    22861 ggggatttat attatggttt ataaccgttg gaataacata tttaacaact tatgctatag
    22921 tgaagctatt tagttttaat atattattga gtattgtagt gaatagtttt ataatttata
    22981 caacactagc cacaaaatgt ttaaaagacg aagcattaaa aatttataat gtattaaaaa
    23041 ccgaagatat agaaaaatct aggattcagc tatcttatat agtaggtagg gatactacta
    23101 atttaagtga acctgaaata ataagagcaa ctgtagaaac tgttgcagaa aatactgtag
    23161 atggtataat tgcgcctatg ttttatgcat ttattggagg agcaccactt gctatggcct
    23221 ataaagctat aaatacatta gactcaacag ttggatataa aaataaaaag tataaagata
    23281 ttggttttgc atctgctaag attgatgata tagccaatta tataccagct agaatatctg
    23341 ttattcttat gacaattgga agtttctttt taaattataa ctataggaac tgttttaaaa
    23401 tctctattag agatagaaag aatcataaaa gtccaaattg tgctttttca gaaggcgcag
    23461 tatctggtgc tctaggaata caactaggag gtaccaacgt ttattttggg gaaaaagtat
    23521 ataagccaac aataggggat aagcttaggg agatagataa agaagatata attaaaacta
    23581 ataaaattat gtatgtatct tcatttgtat ctatgttggt atttacacta atacatgtgt
    23641 tatgtaggat gattttaatt aacttaatta tataaattat tgtttgctaa cttaaggggg
    23701 acatagatga aggatttagg gcatggtgcc aatgtaggtg aaatggcaag attatatggg
    23761 aaaaacccaa aagaaataat tgattttagt tcaaacataa atccaaatgt attaccaaat
    23821 ttagagagat acattttaaa aggattagaa gagtgtagga attatccaga tataaattat
    23881 acaaacttga gagaaaatat atcaaaatat ataggtataa atccagactt tataatacct
    23941 ggaaatggtg caacagaagt gatttatttg cttatgaaga gtgttaaaaa aaaattggct
    24001 ataataaatc cgactttttc agagtataga agaagtgctg agttaaataa tttagatatt
    24061 atagatttag agctagattt agaaaataac tttaaactta atatagatat aataaaagaa
    24121 aatataaaaa gatttgatag tttatttata tgtaatccta ataatcctag tggtaatgtc
    24181 caagatttaa aggaacttgt tcatttacta ggtaaacata ataaggtact aataattgat
    24241 gaaactttca tggaatttgt tgaagatgaa agtaaataca gtttggttaa atatatagaa
    24301 tcaaataaaa atatatttat aattaaagct gtgactaagt tttttggtat gcctggttta
    24361 aggctaggat atggtcttac aagcaacact gaaattatga acaaaattta tgaacacaaa
    24421 gagccatgga ctattaattc ttttgcagat atactatcca attttatttt tgaagataaa
    24481 gagtatatta aaaatagtaa agaatattac atagaagaaa gaaaatatat gttacaagag
    24541 ttgagaaaca taagaaatat aaaggtctat gatacagatg ctaattttat tttgataaga
    24601 gcatataaaa aaacaactaa agaattgaaa aaagatttgc ttaagcaagg aaacatatta
    24661 gttagagatg cgtctaattt tataggatta gatgatagtt ttataagagt ggctataaaa
    24721 tctcatgaag ataacaaaat tcttatagaa aatatgaaaa atttattggg tgattaaaat
    24781 gaaatcttat ggaatatgcc cagcatcttg tggagaattt gtacaaggta taattgatga
    24841 tgaagaatat ttatgttcat atgcaataga tatgtattct gaagtgtata ttgaagaaaa
    24901 attagtagat ataaatttag ggagatataa gtcaaggtta gcaattgaaa aggtatttga
    24961 aaaatttaat ttacctaaaa aatatactaa aaatatttct ttaaatataa atagtaaaat
    25021 acctgttgga aaaggaatgg caagttcaac tgctgatata ggagctacaa taaaggcaac
    25081 attatcattg atagataaag atttaagtag tgaggagatt tctaaattag cagcagaaat
    25141 agaaccaaca gattctatat ttatagataa aaatagtata tttaatcctc taaatggaac
    25201 tgttataaaa tacctaggaa atctgaccaa tgcgaaagtt ataatacttg aaccaaacaa
    25261 agtactagat actatgaaaa ttagattgag gcaagattat aataaattaa aagtagaaaa
    25321 taaagaagta ataaaaaaat catttgctct tttagaagaa ggattgaaaa aaaataattt
    25381 atctctagtt ggagaggctt gtactttgag tagcttagca aatgagaata tagaaaaaaa
    25441 agagtattta aatgaaataa taaaaatatc aaaaaaatat ggagcatatg gagtaaatat
    25501 tgctcacagt ggaactgtag taggtatttt aattgataag tctatgaatg ataaaaagat
    25561 gatagatacc ttatgtgaat ctaacataaa ttctgtctat aataaaattt atacacaaaa
    25621 cataattaat ggaggaatta agggggaaat agaatggaat acataaagaa tcctatgaaa
    25681 atagaagaaa aaagttttga attgatacaa gaaataatag acgaaattag accagattat
    25741 aaatttaaga ataatattga agaaaaaata ataaaaagag ctatacatac tactgctgat
    25801 tttgattact tagatatatt aaaaatatca gaagaagcag ttgatagtat aatagatgca
    25861 ttaaaaaata atgcaagtat ttatactgat acaaatatgg ctcttagtgg tataaataaa
    25921 agaaagttag aatcattagg atgtaagtat aaatgtttag tggcagatga tgaaactgta
    25981 aaattagcta aagaaaaagg aattactcgt tctatggcag cagtagaaat tgcagctaaa
    26041 gaagaaggaa gaaaaatatt cgttttaggt aatgctccaa cagctttata taaagttatg
    26101 gagatgaaat cagaaggtag attagattta gatgcagtta taggagttcc tgtaggtttt
    26161 gtaggagcac aagaatcaaa agatgaagtt gaaaaaacag atataccata tataatttct
    26221 aaaggtagaa aaggtggcag taatttagca gcagcaatag taaatgctat attatatact
    26281 atgtaggtac ctatatggaa gaatatgtat atatagatgg aaaaaaatat agaagaggat
    26341 atactacagg ttcatgtgca acaggtgcat ctaaggctgc tgtatatatg ttgattacaa
    26401 aaaatagaat caatactata aatatagata ctcctaaggg gataccatta ctactaaaag
    26461 ttgataatat aaatatttct gatacatttg tggaatgttc cataaaaaaa gatggtggag
    26521 atgatataga tgcaactcat accatggata tttatgcaag agctgaaatt gtagctaaaa
    26581 atgataagaa caaagggtat cttactctaa aggatataga tagtttaagc accaatagtg
    26641 agtgtaaaag tgagctctat aagtttatta gggtttatgg cggaactggt attggtgtgg
    26701 ttacgaaaaa agggcttagt gtggatgttg gtaaacctgc tataaatccg actccactta
    26761 aaatgataaa ccatgaaata agaaagctta taggagataa ttttgaatct atacttggaa
    26821 atgataaagt tttaaaaata acaatctttg ctccacaagg agaaactgta gcaaaaaaaa
    26881 catttaatcc aagattaggt atagttggag gaatatctat aataggtaca actgggattg
    26941 ttgaacctat gagtgatgag ggttggaaga agtctctttc tatagaactt cagatgaaaa
    27001 aagaacaagg cttagataaa ataatattgg ttccaggaaa tcatggagaa cagtttataa
    27061 gagaaaaatt aaacttagat attaagtatg tagttagggt aagtaatttt ataggctata
    27121 tgattaaaga agctcaaaga ataggatata aaaaaatctt aatggcaggt catataggta
    27181 aatttataaa agtatctgct ggtattttta atactcatag taaggtagca gatgctagaa
    27241 gtgagatatt agtggcaaac ttagctttaa tgggagctag atacgaattt ttaaataaaa
    27301 taaatcaatg cgtaactact gaagaagctg tagagctaat aaataatagt gaatatagag
    27361 aagtatataa tatattaagt aataaatgta aggagagagt aaagcaatac ttgaatgaag
    27421 attcagatga tattgatgtg gaagtaatca tattttcaat ggataagtct ttattaggta
    27481 aatcggataa tacagatgat ttagtggagg tctttatatg ataaacataa taggtctagg
    27541 acctggtaac ttagactata taacaaaaaa aggtgaaaat ttaatttcca cttctgatgt
    27601 attaattggt ggtaaaagaa atctagagtc tataaaaaat tttgaaggag aaaaaatagt
    27661 tctggattct aatttaagag aaattataga gtacataaat aacaataaag aaaagcaaat
    27721 atcaataata gcatctgggg acccattaat atatgggata ggtagatatc tatctaaaaa
    27781 tattgataat aagatgttaa acgtggtttc tgggattagt tcattacagt acatattttc
    27841 aaaaatatat gtagatatga atgatgtata tattacaagt agtcatggca aaataccaga
    27901 ttttgattac atattatcac ataaaaaagt atgtatggtt acagattcaa agataggacc
    27961 aaaacaaata agtagagaaa taatagatag aaatctaaat aaaattattg ttgtaggaga
    28021 aaatctgtca tatgacaatg aaaaaataac aatagcgaaa ccagaagaga taataagaat
    28081 ggacaatttt gatatgaatg ttgtggtgat tttagatgag gaatagtgaa tttataacag
    28141 gtaaagttcc aattactaag gaagaggtta gagcaatatc aataagtaag ttaaatttag
    28201 taaatgctaa aaacctcata gacatagggg ctggtacagg aagtgtaagt gttgaagctg
    28261 catataatta tcctaattta aaggttatat ctatagagaa aaatgatgac gctatagagc
    28321 ttatagagaa aaatataaaa aaattcaact taaaaaatat agaagtgata aaaggatatg
    28381 ctccaattaa tctagggtta aatgttaaag ttgagtctat ttttcttggg ggaacaggaa
    28441 ataatctgga agaaataata gaatggtcta aaaaaaatct cgttacagga ggaagacttg
    28501 tagctaattt tataataata gatacattta atcaaacttt aaaactgctt aaaaagcatg
    28561 gatttaaaga aatagatgta tgtgttttga atgtttctaa actagagaaa ctaggaaaag
    28621 gtgaatattt taaaccacta aatcctatat atataatatc ttgtgaaaaa ggggaaaatg
    28681 atgatgaata aagtacattt tgtaggagca ggacctggag ataaagagct gataacatta
    28741 aaaggataca aattattaag taatgcagat gtagtgatat atgcaggttc acttgttaat
    28801 cctgaacttt tggaatactg taaagaagat tgccaaatac ataatagtgc acatatggat
    28861 ttacaagaga taatagatgt tatgagagaa ggtatagaaa ataataaatc tgttgtaaga
    28921 ctgcaaacag gtgatttttc tatatatggt tcaattagag aacaagtgga agatttaaat
    28981 aaattgaata tagattatga ctgtactcct ggagtaagtt cttttttagg ggcagcatca
    29041 tctttagggg ttgaatatac agttccagaa atttctcaaa gtgtaattat aacaagaatg
    29101 gaaggcagaa ctccggttcc agagaaagaa tcaatacaat catatgctaa gcatcagaca
    29161 tctatggtaa tatttttatc tgttcaagaa attgagaaag ttgtgtctaa attactggaa
    29221 ggtggatatc caaaagatac tcctatagca gttatataca aagcaacttg ggcagatgaa
    29281 aaaatagtta aaggaacttt aagtgatata gctgtaaaag ttaaagaaaa caacataaat
    29341 aagactgctt taataatggt tgggagattt ttaggagaag agtataataa ttcaaagtta
    29401 tatgataagg atttcaaaca tgaatataga gggtaatatt gctataatct gtattacaga
    29461 aaatggaaaa aatttagctg taaagataaa ttctctagta aataactgtg ttatatatca
    29521 agtaaaaaat aaaaaaagta atttagttgt tgaagaaaca tgtgtacata cagttcaaaa
    29581 gaagttaagt gattttgtag gagaaatatt tagcaaattt gattacataa tatttataat
    29641 ggctacaggt atagttgtac gaagtatagc accatacata ataagtaagt ttagtgaccc
    29701 tgcaattttg gtaatggatg aaaaaggcaa aaacgtaata agtttattga gtggtcatat
    29761 tggaggggca aacagcttga cacagtacat aagtagtctt attgatgcta atccagtaat
    29821 aacaacagct actgatataa atcaaaaagc ttccttagat atgatagcaa aaaaactaaa
    29881 tgcacatatt gataatttta gagaaagtgt aaaagaagta aattctatgc tagtgaataa
    29941 taaatgtgtt ggattatatg ttgatgaagg atatcaagtt gatactaggg gatttaaact
    30001 tatagaaagt aaggatgagt taagagatag cttttcaaac acatatatag aaaaattagt
    30061 tgtagtaagc aataaaataa attgtattga agatattgag aatttaaact tagataataa
    30121 atttgaacat tgctatagta ctaaaaaaat aataaaggta attccaagag atatagtgat
    30181 aggaattggc tgtagaagaa acacagatag tgagctgatg tataattcac ttgaagagtt
    30241 tcttaataaa caaaatatag atattagagc aataaacaca gttggaagta tagaactgaa
    30301 aaaagacgaa aaagcaataa ttgaattatc aaaaatacta aaagtgccat ttaaaacatt
    30361 ttcaatagat gaaatatcta aggttgataa tctttttgat aaatctgatt ttgttaaaaa
    30421 aaatgtgggc gtgtattcag tagcagaacc tatagcatat cttttgagca aaggtaattt
    30481 gttagttgaa aaacataaat ataaaggaat aactttttca gtggggagaa taaaaatatg
    30541 atatacgttg taggaattgg accaggaagt aaagatacaa tgactttaga agcaataaaa
    30601 gcaatagaag attctgaggc tattgttgga tataagactt atataaaact aatagaagaa
    30661 tttatacaag gtaaagaaat aatccaaaat ggtatgaggc aagaagttga tagatgtaaa
    30721 caagcagtag aggaagcgaa aaaaggaaaa aaagtagcag ttgtaagcag tggtgatgct
    30781 ggaatatatg ggatggcagg tctcatttta gaattaatat ctaaagaaga tgaagatata
    30841 aaagtaaagg taattccagg tgttactgca agtattggtg cagcagctat acttggagct
    30901 cctattatgc atgatttctg tcatataagt ttgagtgatt taatgactcc gtgggaagta
    30961 attgaaaaaa gattgagatt ggctgctgaa gccgattttg taatttgttt gtataatcca
    31021 agaagtaagg gaagaagtga acatctaagc aaagcattta aaataatggg tgagtttaaa
    31081 ggtggagata cacctgttgg agtggtaaaa gatgtaggta gagaaaaaga agaaaagttt
    31141 gtatgtactt ttgaaaatat ggactttgaa aaagtcgata tgactacaat ggttataata
    31201 ggaaataagt ctacctatat taatggtgaa ttgatgataa ctccaagagg ttatacagta
    31261 tgattttaat tttaggagga acttcagata gtttatctat atgtgataag ataaatgaac
    31321 tgaaaaatca accatatatc ctttcagtta ctacagatta tggaagggaa cttgcacaac
    31381 aacatgcaga gaatgtaatt ttaggtaagc ttggaaaaga agatatgcta aaatttataa
    31441 aagataataa tatagtaaaa ataatagatg ccactcatcc ttatgcagtt gaagtatcca
    31501 aaactgctat tacttgtgca aaattattga atatagatta tataaggttt gagagaaaat
    31561 ctttaattga agaaatagat tatgataata aatttatagt agatacaatt gaagaagcct
    31621 gtgaaatagc aaataaaata gggacaaata tattcatagg aacaggaagt aaaaatttga
    31681 acagatttat agaggaaata agtgataaaa atttagttgt tagagttctt ccaacgagtg
    31741 aagtcatatt aagttgtgaa aaattaggtt taagtgcaga taatatagta gctatgaaag
    31801 gtccatttag ccagtatata aatgaagaaa catataaaca ttacaatata gatttagtca
    31861 taacaaaaga aagtggaata gctggtgggt tcttagagaa ggtaaatgca tgtaaaactt
    31921 tcaatatacc agtaataatt ataaaaagag aatcgattaa ctacgaaaat gtatttaatg
    31981 acataaataa aattaaagac atacttgtgt aatttttagg aggagaatat atgaagaaag
    32041 ccttattagt agtgagtttt ggtacaagct atcatgaaac gagagaaaag acaatagata
    32101 tatgtgaaga taaaataaaa aattcgttaa aaagccatga tttttttaga gcttatacat
    32161 caaatatgat aataaataaa ataaaaaaaa gggatggaat agaaatagat aatccaatac
    32221 aggctttaga taaaatatat gaacaaggat atgatgaagt aataatccaa acacttcata
    32281 ttatatgtgg cgaagaattt aacaaattaa aagaacaagt agatggatat tcttctaaat
    32341 tcaaaaagat tgtattagga agacctttgc ttacacatat tgaagattat caggaagctg
    32401 tagaggcaat taagcatcaa attcctcata tggaaagtaa tgaagcagta gtatttatgg
    32461 gacatggaac acttcatgaa tcacattcag cgtatccagc attggaatac atgcttaggg
    32521 ataatggaat aaatgcttat gttggtacag ttgaagggta tccagaaata gaacatgtaa
    32581 taagaagatt aaaagaagga aatataaaaa ctgtaaattt gatgccattt atgttagttg
    32641 caggagacca tgctataaat gatatggcag gagatgaaga agattcttgg aaaacaatat
    32701 tagaagaaga tggatttgac gtaagagttc atttaaaagg actaggagaa aattcttata
    32761 tacaagataa atttgttcgt catgctgtaa aatgtgcaga gaatgctaag ttctatggta
    32821 taggagcagg gccaggagat ggttctttac ttacgataaa agcggttaat actttaaaga
    32881 aactggatat tctatacact ccagaatcaa aaaagggtgg agatagtcta gctttatcaa
    32941 tagttagtga atatttacct gaatcagtgg aaataaagtc taggcatttc ccaatgagtt
    33001 ttgatgggaa tgaaaaaagt ttagcttggg acaatgtagc acaagaaatt atatcagatg
    33061 ttaatgatgg taaaaatgta ggatttgtta cattaggaga ccctatgatt tatagtacat
    33121 atgtatatat aatgaaaaga atattaggag aggtagaagt cgaaactata ccaggaattt
    33181 catcattttc caatatagca tctaatcaga attttccatt agtcatggat aaagaagctt
    33241 tagtggtaat tccttgtact atggaagatg ataagataga gtatgctttg caaaattata
    33301 attcaatagt attgatgaag gtatataaaa attttaaaga aataataaaa aaattagaaa
    33361 agtatgattt aatagaacat gctattttag taagtaattc ttcccaagaa agcgaaaatg
    33421 tgtttacaga tttaaaagaa gcgcaccttg aagataagat atcttatttt tctactatac
    33481 tagtaaataa agataataaa ataaaatagg tgaaagcaat atttaaagga gacttaatat
    33541 gaatatagtg gttggaacta ggggtagtac tctagcgctt atacaaacag agtgggtaat
    33601 aaatgaatta aaaaagaagt atccagaaat tagttttgag attaaaatca taaagacaaa
    33661 aggagattta attcaaaatg tttcactgga taaaataggt gataaaggat tgtttgtcaa
    33721 agaaatagag caacaattat tagatggaaa aatagacata gcagtacata gtatgaaaga
    33781 tatgccttct tatttagcag atggactaaa atttgctcat acgcctaaga gagaagaccc
    33841 aagagatgtt ctaatactta gagaaggata taaaaattta gatgatttgc ctcatggagc
    33901 agtaatagga acaggaagta aaagaaggaa gtttcaatta ttgaaacaaa gacctgattt
    33961 aaatatagta caagttagag gtaatgtgga aactaggata cgaaaaataa aagatgaaaa
    34021 catgcatggt attgtgttgg cagcatcagg aataattaga gctaatttgc aagataaaat
    34081 aagttcttat ttacctgttg atgttgttat tccagcacct gcccaaggag ctttagctat
    34141 agaaattaga agcaatgata gtacgataga gggaatagta aattcactga aggatgagaa
    34201 tacagaaatc caaatattgg ctgaaagagg ttttttagat ggtgtaaatg gaagttgtca
    34261 tattccaatg gcagcttatt gtgaaataat ccaagataaa atacatctta ctggcttata
    34321 tggagattca gaaggaaaaa aagtagtaat caagtctata gatggagata tttcttctcc
    34381 tagagagtta ggtttaaagt tagctaaact tgtattgaag gagtatgaga attatgaagg
    34441 gtaaggttta cttagtaggg gcaggaccag gtgattataa actcatgact ttaaaaggtt
    34501 tagagtgtat aagaaaatcg gatgtaatag tatatgatag attagcaaat agtagttatt
    34561 taagagaagc taagcctgat tgtgaactta tatatgtagg caaggcatca agtaatcata
    34621 ttttgccaca agaagatata aatagaatta tagcagaaaa agctaaagaa ggaaaaattg
    34681 taactcgtct taaaggagga gacccttatg tatttggcag agggggagaa gaagcagaga
    34741 ctttagtaga tgaagatata gaatttgaag tagttccagg tataacttct gctataggag
    34801 gtttatgtta tgcgggtatt cctataacac atagagacca tgcttcgtca tttcatgtaa
    34861 taacaggtca tttgaagggt gatgatagtg gagaattaaa ctggaatgct cttgcaaata
    34921 acaaaggtac tcttgtgttt ttgatgggaa tttcaaacct agagaaaata agtgaaaact
    34981 taatgaaaga aggaaaagat aaggatacac ctgttgctct tataagttgg gcaactagat
    35041 ataatcaaag agttgttaca tctacacttg agaatgttta tgaaactgct attaaagaag
    35101 aagtaaaacc gccaactctt atagtagttg ggagtgttgt aggacttaga gaaaagttga
    35161 acttctttga aagtaagcct ttatttggaa aaagtattgc tgtaactagg tctagaaatc
    35221 aaaatagtgt attggtagaa aaaataatgg atttaggtgg aaatcctata gaaataccta
    35281 ctattaaggt agaaaaaata caaaataata ttaacttaga aaatgaaata aaaaatataa
    35341 ataagtataa ttatttaata ctaactagta aaaatgccgt agaaatcttc tttgaaaaga
    35401 tatatgaaat gaattttgat ttaaggatat tatctaattt aaaggtatgt gctataggtt
    35461 ctgctacatc aaatgagttg aaaaaaagag gaattatagc ggatatagta ccaaaaaaat
    35521 ttgtagcaga gtcactgtat gaagagttag ctcctatttt aaatgataac gataatatac
    35581 ttatacctag agcagaaaat gctagagatt atctggttga caaacttaag gctatttgtg
    35641 aagtaacaga agtacatata tatagaacag taatagatga ggataaaaaa ggtgagatat
    35701 tagatatttt aaattctaat gatatagatt atataacatt tactagttcc tcaacagtaa
    35761 ataatcttgt tgaaattata ggaaaagaaa atattaaaat attagaaaaa ataaaaacac
    35821 tatctatagg tcctataact acagagacaa taaatagttt gggaataaaa ttgtacaaag
    35881 aatctgctac atctactata gattcgttag tagatttaat tataaaaaat taattttgga
    35941 ggcttgaata tgttaagaag accaagaaga ctaagagcaa gtaaagcaat cagaaatttg
    36001 gtaagagaaa ctaaggtgaa tgtagaagat ttaatatatc cactgtttgt agtagagggg
    36061 gaaaatataa aagaagaaat caattcttta ccagatgtat atcatttatc aatagacatg
    36121 ctagaagacg aaataaaaga aataagtgag ttgggaattg aacatataat gttatttggt
    36181 gttccagatg aaaaagatga gtgtgcttca caagctttta atgataatgg aatagttcaa
    36241 aaggcaatta gaaaggtaaa agaaataaat tcagatataa atgtaataac agatgtctgt
    36301 atgtgccaat atacaagtca tggacattgt gggattttaa ctgatactgg ttatgttgat
    36361 aatgataaat cattggagta tttggctaaa atatctgtaa gccatgctaa agcaggagct
    36421 gatatggttg ctccatctga tatgatggat ggaagaatac aagcaataag agaagcttta
    36481 gatgaaaatg gattagaaaa tgttggaatc atggcttata gtgttaagta tgcttcaaat
    36541 tattatggac cttttagaga ggcagctcat tcagcaccat cttttggaga tagaaaaaca
    36601 tatcaaatgg acccagcaaa tagcaatgaa gctataatag agactgaact tgatatattg
    36661 gaaggtgcag atatacttat ggttaaacct gcattatcat atttagatat tataagaaga
    36721 gttaaagata attttgactt acctctagca gcttataatg taagtggaga atactctatg
    36781 cttaagtttg ctgtgaagga aggtttatta aatgaagatg taattcttga atcaattaca
    36841 tctataaaaa gagcaggagc agatataata ataacttatt ttgctaaaga cattgctaaa
    36901 ctgttgaaaa aataaattct agtagtataa agaatatcaa aaagtatagg aattgtaatt
    36961 aactattata cagtagtttt gtttagacat ttattgaaga ataatagaaa ctaatttaca
    37021 atttctatac tttatttatg taactaaata aaatttggag gtagaagatg ctatatccta
    37081 ttaatttaaa attagatgag ttagatgtag ttataattgg tggtggagaa gttgcatata
    37141 ggaaatgtaa aaatttttta gagtttaaca aaaatgttac tatagtttct aaacaaattt
    37201 taaataaatt ttatgattta aaaggaaata taaaaataa
//
LOCUS       DAEPOG010000016        37223 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEPOG010000016 DAEPOG010000000
VERSION     DAEPOG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.016"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(72..275)
                     /locus_tag="KR432_000724"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="KR432_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="KR432_000725"
     CDS             complement(275..604)
                     /locus_tag="KR432_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBH0639279.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="KR432_000726"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="KR432_000726"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBH0639280.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="KR432_000727"
     CDS             complement(1995..3626)
                     /locus_tag="KR432_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639281.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="KR432_000728"
     CDS             complement(4109..5146)
                     /locus_tag="KR432_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0639282.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="KR432_000729"
     CDS             complement(5165..5950)
                     /locus_tag="KR432_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0639283.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="KR432_000730"
     CDS             complement(5983..7116)
                     /locus_tag="KR432_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0639284.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="KR432_000731"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="KR432_000731"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBH0639285.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="KR432_000732"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="KR432_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBH0639286.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="KR432_000733"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="KR432_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBH0639287.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="KR432_000734"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="KR432_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBH0639288.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="KR432_000735"
     CDS             12247..13245
                     /locus_tag="KR432_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBH0639289.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="KR432_000736"
     CDS             13607..14134
                     /locus_tag="KR432_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBH0639290.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="KR432_000737"
     CDS             14161..14757
                     /locus_tag="KR432_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBH0639291.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="KR432_000738"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="KR432_000738"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0639292.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(16971..17807)
                     /locus_tag="KR432_000739"
     CDS             complement(16971..17807)
                     /locus_tag="KR432_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0639293.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(17831..19297)
                     /locus_tag="KR432_000740"
     CDS             complement(17831..19297)
                     /locus_tag="KR432_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0639294.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(19300..21162)
                     /locus_tag="KR432_000741"
     CDS             complement(19300..21162)
                     /locus_tag="KR432_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0639295.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(21409..22272)
                     /locus_tag="KR432_000742"
     CDS             complement(21409..22272)
                     /locus_tag="KR432_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0639296.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(22327..23259)
                     /locus_tag="KR432_000743"
     CDS             complement(22327..23259)
                     /locus_tag="KR432_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBH0639297.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(23345..23896)
                     /locus_tag="KR432_000744"
     CDS             complement(23345..23896)
                     /locus_tag="KR432_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639298.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(24125..24616)
                     /locus_tag="KR432_000745"
     CDS             complement(24125..24616)
                     /locus_tag="KR432_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639299.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(24644..25204)
                     /locus_tag="KR432_000746"
     CDS             complement(24644..25204)
                     /locus_tag="KR432_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0639300.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(25356..26513)
                     /locus_tag="KR432_000747"
     CDS             complement(25356..26513)
                     /locus_tag="KR432_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0639301.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(26550..27491)
                     /locus_tag="KR432_000748"
     CDS             complement(26550..27491)
                     /locus_tag="KR432_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639302.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(27578..28270)
                     /locus_tag="KR432_000749"
     CDS             complement(27578..28270)
                     /locus_tag="KR432_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0639303.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(28380..29192)
                     /locus_tag="KR432_000750"
     CDS             complement(28380..29192)
                     /locus_tag="KR432_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBH0639304.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(29243..30154)
                     /locus_tag="KR432_000751"
     CDS             complement(29243..30154)
                     /locus_tag="KR432_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0639305.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(30213..30944)
                     /locus_tag="KR432_000752"
     CDS             complement(30213..30944)
                     /locus_tag="KR432_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH0639306.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            31033..31284
                     /locus_tag="KR432_000753"
     CDS             31033..31284
                     /locus_tag="KR432_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639307.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            31517..31936
                     /locus_tag="KR432_000754"
     CDS             31517..31936
                     /locus_tag="KR432_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639308.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            31961..37018
                     /locus_tag="KR432_000755"
     CDS             31961..37018
                     /locus_tag="KR432_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBH0639309.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
BASE COUNT        12270 a         6338 c         4461 g        14154 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattctt taagaaattc
    16681 tgaagatttt ataaagtttt ttgtattata attaattaaa aataaaattc tagttataaa
    16741 acatattttc cattaaacaa aaaaatagtt aaaataaatt cataaccaca taaacttatt
    16801 ttaactattt gtatatttta attataaata ttaacttaaa tttttaacag gatacactca
    16861 taaaataaac ataatgaaca acaaaataat atgtctcagt ttctttatat actttacctt
    16921 ctttcaattt tctaccatgt agacgtataa gataatatta gtaattcact ctattgctta
    16981 actcgtgcga tatggacaac caagaaagct atttcctcat ttgttaactt ttttccatat
    17041 ttcttttcta aaaaatctct aacttttaat gcaattaaca tttcttttgg atttgacctt
    17101 ttcatttgat taaataataa atcatcacca ctgcttaaca tactatctga aaaaaatctt
    17161 tccacaaaaa attttatatg tgttataaat ctcatataat gcatactctt tttatctaag
    17221 gttctattca aagaaaaaac aaaaatatta attatttctc caattacttt ggcatactta
    17281 atcgtatcat atgaatttcc atctgaagcc atagcatttg caaaatggaa agcaatgtta
    17341 gctgcttctt cctctggtaa ttgaatgccc aatctctcct ctaccatata aattgcaacc
    17401 attccaacct taaactcatt tggataataa ttttttattt cccaaaatac tcgatttgta
    17461 atttttattc ctttacgcat cctttcaatt gcaaagttaa aatggtctgc taatattaag
    17521 tataagtttt tattaaaaga tgagttcaat aacttttctg cctctactat tatttcctgt
    17581 gttatcttta aaatgtcgac aggaatatca tccattaatt ctaaaaattt ttttgatttt
    17641 gtattttcaa cagggacaaa catttgatta tcattggaac cacaaatata ttctcctgtt
    17701 tttttaccat atccaattcc tttccctagc aaaataaatt cattatactt tgcatcatga
    17761 actaaaacaa cgctcgagtt caaaactttc tttattatgt agttcatttt atttgctcac
    17821 cttctattgg tcatttaaaa tctatactat tattttcaat tactttttta taccaataaa
    17881 aactatcttt cttatatcga ttatatgtcc cctttccata atcatcagca tccacataaa
    17941 tatatccata acgttttgac atttgtttcg ttgattcact gaccaaatca atacaacccc
    18001 atgaagtgta tccccaaagt tcaacgccat cttcgataat cgcatctaac atacatttaa
    18061 agtgttcttt taaataatta attctatatt ggtcatgaac ttttttatct tcagttaaaa
    18121 tatctttagc tcccaatcca ttttcaacta taaataaagg ctttctgtat ctatcatata
    18181 attcaatcaa agatatacgt agacctattg aatcaatttg ccaaccccat tcactcattt
    18241 ctaaataagg attttttaca gctaataacg tattcccagg tgtaatatct aatccagttg
    18301 tatcagctgc ctcacaagaa gacatatagt aagagaatga aataaaatct actggatact
    18361 ttttcataat ttcatcatca tgttcttctt ttgctatttg aatgttatta tttttaaaat
    18421 acgaaagtaa atatactgga tactcaccaa aaacctgcgt atcactatat gcataaatac
    18481 ttctcatttt ttgttgagtt gccaaaacat cttctggtct acatgtatat ggataatacg
    18541 ttaattttgt taacatacac cctacttttg aatttggaat aatctcatgg caaatttttg
    18601 ttgcaagggc actagctaca aattgatgat gcatagcttg aaaaataact tcctcaaagt
    18661 ttttatcctt aaaacggtct cgaactaaac caccagtagt gtacggatga cgaatcatag
    18721 aatcaacttc attaaatgta agccaatatt tcactttgtt tttatatctt tcacagatag
    18781 tttctacata cctaacaaac atattgataa cttgtcgtga ataccatcca tcatagttta
    18841 aaacgatatt taatggtggt tcataatgtg acatagtaac aagtggttca attccataac
    18901 ttatcaactc atcaaataca tcatcataaa actgtaaacc ttcttcattt ggctctttat
    18961 catctccatt tggaaaaata cgagaccaag caatactcat tcgatatgtc ttaaatccca
    19021 tttctgccat tagagcaata tcttctttat agtgatgata aaaatcacta ccatgacgtt
    19081 ttggataatt tacttcatct tcaggatgtg ctaatccttc ttctatatct tttgttgtga
    19141 tttcattatt agatttatag tctttcacat ctgcatcaaa gtgtgccttt gctacatctg
    19201 atacagacca ccctttacca cctaaattcc aaccaccttc aaattgatta gcagctgtag
    19261 ctcctcccca aagaaatcct tctggaaact ttctcatatt tatattcctc cctttacatc
    19321 aaacaagata cttccttttt ttacattgcc ataagactgg tttaacactt tataagcata
    19381 atctgctcca ttattgttta caatcataac agttgaatct aaattagcat gtgagatttt
    19441 gtctaaatca aattgaatta ataaatctcc ttcttttaca ttatcgcctg tcttaacaaa
    19501 tgttttaaat ccatctccat tcatacttac agtatttatt ccaatatgaa tcaaaatctc
    19561 agccccattt tttgttctta aaccaattgc atgttttgtt tcaaataaca tcacaacttc
    19621 tgaatcacaa ggtgcgtata aatttccatt ggtcggtata attgcaactc catctcccaa
    19681 agtcttactt gcaaatagtt catcattaac cttacttaaa tctattactt ttccttctac
    19741 tggagttact aagatattta cttctttttc ttcttctagc tcttcttcaa caacatctcc
    19801 aattgatgac tctactgtat ttgtatcttc cttccaaata atcagtgtaa taataaacga
    19861 gcctaatatt gcaataacaa aaccaatact cgcaaaaatc aaattgtttc cattgtttgg
    19921 gtcaataaac atactcatac tggcaagccc tggtccaaca attacaaatc cttttactgc
    19981 aaataatcct aaaaatgtac cagaaattgc acttgagaat acaacaccta acattgctct
    20041 tttatgttgt aaagtaatac catataaagc tggctctgta attcccatca aggcagaaat
    20101 tcctgcagat aatgctgttt gttttaatgt ttcatctttt gttcgcaagg ctactgcaaa
    20161 acaagcacca ctttcagaaa tattgtgggc taatgaagat gtcatatata aagcttcata
    20221 acccaacttt ccaattgtat taattgaata aggtattagt gctttgtgca ttcctgttgc
    20281 aaccataaat ggaagaaaag cagcaagtaa tcctacaact aagaatccca ttttgttata
    20341 taagaataaa ataatggttg taaaaattgt accaacgtta tatcctaaag gacctaaaat
    20401 agttagagta attggaacag taattgccaa agacatcata ggaacaaaga aagttcgaat
    20461 tggttttggt gaatacttgt taaaaatttt ctccattgtt gccaaaaaag acacacataa
    20521 gatagccggg aaaacttgtg cattataggc aatgtttgga attgtaaatc caaatagttc
    20581 gacaccttca cttaaagcag ttgttgttgc tggaagcaat aagtatccta cagcggcaat
    20641 agctactaaa cgattacatt tcaatacatt ggcagtagtt acagctacca taagaggtag
    20701 gaaatagaat gttgcatctg aaatagcaac taataatgta tatatagtag aatctgattt
    20761 taaaatatta aacattgcta ataacatcaa cagtgatttt aaaataccag ctcctgcaat
    20821 agcaaaaatc agtggttgaa aaatattaat aataaattct ataaatcttg ttcctaagtt
    20881 tattttttct tcagttttta cagtactatc atttgtatct aaagggcaaa cttttaaaat
    20941 ttcatcatac acatcaacaa cactatttcc aatgacaact tgaaattgtg ccccattaac
    21001 tatactcata acaccttgta ctttttttaa agcatcctga ttgactttac ttctatcaat
    21061 caacttaaag cgtagtctag ttgaacagtg ttctaaagct aaaatatttt ttgaaccacc
    21121 aacttgttca ataatgtcaa tggcagtttt tttataattc atcttgtaat ctccttctcc
    21181 ataatttttt atgaatggcc attcaagatg caaaaaaggc agaacctata taaacacctt
    21241 gaaaaatcaa agcaatatat tcgatcctgc ctgcattacc agtaacatgt cttctattta
    21301 tactattttg tattattata ttaacatttg cctaaaagaa ttgtcaatac ttttttaaat
    21361 tttctgatac taactaatag cacctttata attcattttt tatgtcaatt aaagtaacag
    21421 tagccaccca ccaattagta tcaaaattga acctggtatt ttttctttta tattttcttt
    21481 gaatataaaa tgagcaagta ttaaagttat tatcatagat acttgagaaa atggctctgc
    21541 tatactaagt tctagattat ttaaagcaat taataatgct aaataagaat atccattaat
    21601 agcaccactt aaaattgaaa gccccttttt attactaaaa atctcacaaa ttattttttg
    21661 tttcttttta aatgctaaaa agataaataa atttaaacct acgaaaaaat acagtatagt
    21721 tgaatataat ataggagata cttgtacaaa aaaagcctta tccaatactc ttcctactga
    21781 ctgcaaaaat atgtataaaa acattaatct acatggtaaa ttgttaaaaa ttacagagac
    21841 attctttaaa aaagagctta ctttttttaa tataaacact ccaccaatca taattatgat
    21901 tcctgcaact tttgctaaac ttaaaccttc acctaaaaat ataactgata aaagcagtaa
    21961 aaacaatgaa tttaaactat ttattggagt aacaagtgaa acttccccag ttgctaaaga
    22021 cgttacatac gctactgaac caattgaata gactaaacta cttatataac atggtattaa
    22081 aaacgaccaa tcactaactc tatccattat gctaaatgga agtaaacata ctccccctat
    22141 aaagaaaaat agaaatgatg aagctatatt tttataaata tctccttctt catcacctaa
    22201 taatcttaca actactcttt caaaagctaa tagcactact ctaattaata atgcaaaata
    22261 agataatatc atttttgacc tcgcttaaaa attaatccga cttattgtta tattaactgt
    22321 ataagactac catataggta agtctttggg attttttaat attttatgtt taaatacagg
    22381 tacattaagt ttttcaccta gtttacggca ttgctcttta taggtacctt ttaatttatc
    22441 atgaaaaact atacaatcaa ttccaccttc aatactattt atacactttt ctagtttctc
    22501 aaatcccgat tcatcatcat acattccact tacaatccat tgatttggca atacatgtcc
    22561 aattgatatg ttatattctt tattttttat aatatctgag aaagatttct caaaatgtac
    22621 ccctccaaca cataataaag tcttcaaagg ttcatcacaa tcaaatactc taattaatga
    22681 ttttgccaat acctgtattg ctatactatt attaaaacta tcactactac ttcctatctc
    22741 aatatccaac ataggtacag aatattctgt aattaattta gattcttcac catatgttgt
    22801 tccagaccag tgagttgctt ctgttaatgt agtaaattca tcaagtagat tttcattttt
    22861 tatatcctca atagcattga ttagattttt aaagtatctt ggatttgaat ttccaaattc
    22921 tcctgttgga acatctccaa ttgtatgagc tgttaggata tgttctggag cattttctcc
    22981 ctcatgccaa tttacaactc ctgcaaaatc ataatctctg aaatgtctat ttaaaattgg
    23041 aagataatat ttataattat gacttattac atcttttgtt tttacaaaat gaaataagtt
    23101 attgttttta tccttaaatt ctaatacaaa attatcatca ataacaatat cagtttgatt
    23161 taaatccatt atttcttcac aacactttat cacattactg gctactgggt ctttctttaa
    23221 atcactacaa aagaaaaaaa ctgctttttt attattcatc cataaatcac actcctataa
    23281 cttaaatata atatatcttc atacctaaaa tcatctaaat atcactctaa ttccattaaa
    23341 ttatttatat ataatatact accatactca ttttagctat acctttgcct gagtttctat
    23401 atatatgtga tacatcagct ttaaatctta tagaatttcc actagataca atgtaatctt
    23461 ctccatttat tttaatggtt acttcgccac taaaaacagt tataaactct tgtgttcctg
    23521 gaagttcagc atttgaatct aaatttccac cttcttctat ttcaatcata aatatctcaa
    23581 atcttctttt ggagtcaaat ggaaatattg gataacttat atactttcca ttatcttcaa
    23641 taaaatggtc tatatcactt ttttgaatca attctatgtc actttcctgc ctctccatca
    23701 atgatgtaaa tgagattttt agtccattag ttattttctt caaagtagat atagtaggat
    23761 ttacctcaca cttttcaatt tgagatagca tactcttact tacaccacta agttgtgcaa
    23821 tcttgtctaa actaagatgt ttttctttcc ttatcttttt taaattttca gcgattattt
    23881 catttaaact ttgcataaat accccctaat acatactaat ataagataaa ttattttgat
    23941 tattaaataa acttagtaat ttaatagaaa ctttttatcc attatactgt acattatgtt
    24001 taatatacat tacttttttt agaatgtaaa tagttttctg aaattttgtg aaagtgaaca
    24061 aataaaaaac tagtaaccta tatttttaca aggctactag cttttattat aataatattt
    24121 cattttaact ataaaactta gatgcaccta taagctgtct cttagaaaca atcttagcta
    24181 ggtttgctaa tgaagaaatt tttatatctt taaaagaaac taaaaataca aacttaaaat
    24241 ctgtaaattc ttgatttata ttgtatatat caaaataaca tctcaaatta tcaaataagt
    24301 aattatcttg taagtctgct tctatctcat cagtagcatc ttttaaatac gctcttacat
    24361 cttcaaataa aacactgaaa taagactcat ctatttcttc acctaactct tccaatattt
    24421 cttctatatc taaatcatcc atatcaaaag aatttcctgt caactggcaa tcaaatacca
    24481 atatattttc ttgatttata gtatttgata tatcttgtac atattcgaag tctggacgta
    24541 attctaaaat gtcttttatt tcactgctct ttacatggat ttttcctttt ctattttcaa
    24601 tttttaaatc tatcatatgc tccccctttc aatctacaaa tctttaaata ccttgtttta
    24661 taatcttttc aatatctatc caattactaa ctctaactat attttgatgt ttaacatctt
    24721 tattatagtt agtatccaga agtattactt taacaccatc atcagctagt tgcactgagt
    24781 ttaaaggatt atcttctata aatatgtcac aactcagttc tcttgccttt tcaactttat
    24841 aatcacttcc taataagtaa acatctatat ctgaaaatcc ttgtctcgat agccaatttt
    24901 tagttacatc agttaaacac tcatgtctag ccgttacaaa agataaatta tgtcctttag
    24961 ataatgtgct aattacctct gtagcaccct ctacaatctt tgcttcatca tatgagtgta
    25021 tatacttatt attaaactcc gcatacacat ctcttattcc cgaaccatac aattcttccc
    25081 aattatgtgt tatacactct gcttcagtta tatttttatt aaagatatca tttaggtatg
    25141 gaagaaaatg ataagggctt gttattgttc catctatatc aatacatata ttcaatttgt
    25201 tcatttttac ctccatgtaa catttattga tttgattaat caaatattat agcacagctt
    25261 taattataaa tcaaaactct ggatatgact atacctatat aataccaaaa agcttgcata
    25321 tttaagcaag cttttcgtaa attttacatt tttaactaat atataagttt ttctaaggtt
    25381 ggttccactc caaattgtga tttcagttct ttatattctt ttaatgcttt gtcattattc
    25441 gttttagttt ttatcgctct tatcattaaa ttttttggtg tatcaagtgg agatatgtat
    25501 tctactacag aagtatcata tccattgccc tccaataaca atgttcttaa accatcagtt
    25561 atcaaatctg caaatctagc tttaaataca ccatgtttta atattggttc catagcttca
    25621 tatctatatt gccctaataa ctccttatga caacatggta ctactactat agcttcagat
    25681 tttgctctaa ttcctagacc tattgccata tctgtagctg tatcacaagc atgtagactt
    25741 attacaaggt caacatccct attaggtgta taattagtca aatcctcttt tataaatgac
    25801 atatttttat aacccaagtt tttagccata ttttttgatg cttctataac tacatctgaa
    25861 taatctattc ctataaaata acagtttttc ttcaatactt cttttatata aaaatttaag
    25921 acaaagctta agtaagattt tccacaagca caatccaaga tagttataca gtctttatcc
    25981 tttatttctt ttaaaatact atctatcaat tcaacaaaat ggtctatctg attgtatttt
    26041 cttattttat cattttttat cttaccatct tttgtaagta ctccaatttc ttttaaaagt
    26101 gcatttgctt gtcctacttt tacataataa tctctgtttt ttatttgaga tgccgtaaat
    26161 tcatctattt taggtacttc ttctttatta tctaaatatt ttacatttac cttcttattg
    26221 tctccttcaa ttacaacaat agttcctcgc tcaacatatt taaatactaa tccatcataa
    26281 ttttctgcct ctttagaaat attcttaagc aactcattaa acgtatataa atctgatttt
    26341 cctttaaaat taaatttata tttattatct tcctctattg caattccttt aaactctttt
    26401 cttccagatt taaaaatcac ctctaactca ataaaaaaat gcctattatc aaggtttctt
    26461 tgagtaatcc ccataaaaaa catatttagt tttcctatac tctgtttatt catcataatc
    26521 tcccttttaa cttatatctt taaatcttat taattaatat tatttgtttc tgaattattt
    26581 atttcttctt ttaaatcgtt caatctattt ttaaaacttt ctatctgctt atctgataaa
    26641 tcaagatatt ctgttatctt ccctatatat ttagggcttt tagtatttat taatttcaaa
    26701 gcactttcca tagatttttc tctaagctca tatgctttct ttacgtcatc tctagcatta
    26761 ctcaatgttt ctgtatactt ctccttagaa agttctggaa tctgcatatt ttcatattct
    26821 tttacaactt ctttacactt gtttctggca tttgtaacat ctttttttat actagataat
    26881 ttagttatat tttttgctat atccttagtg taaggtatac attctctcat tggaactaaa
    26941 gctgctcttt ctatattata aatttctttc ttaaaaattc ctatactttc catatctatt
    27001 ttttttatat cttcactatt tttttcttta cttgtatatt tattattttc ttttgtatta
    27061 tcttcttgtt tttctgattt attattcact gaaacattac tatcagaatt attcttttct
    27121 tttatatcat tacttccttc ttcattactt tctttatttt gtatatttct atcttgtttg
    27181 ttgtttaaat ttttattatt tgatatttct tcattctttt tattatcctg ttttaaatcg
    27241 gtactttctt tttccttaaa atttgataca tacttttcat catatgtctc atcagaaata
    27301 taattgtgat aatacataga ctcttttatt ttattaagtc cactcatata aatattactt
    27361 atcattattg ttgtagctaa taccacataa atactactgt agatacaaga cctcttaaaa
    27421 ttcttgtaat ttctgaatat cactactaaa ggaataagat ataaaaatat aaaaactaat
    27481 atccacctca taagtcgtgt ctcctttctc aaaaccgaaa tccctcaaat atttattttt
    27541 aatcatgtat cttatagtat ttaccaattt atgtttttta atcgtgaact tttattaaat
    27601 taaattctgt atagtcacaa gatgctaatg tatattctac tccatttcga atccccttca
    27661 atcccatttt aaaagattct ttaccatgca agtgaccata aatcaccttt tcaacattat
    27721 attcttcaaa taactttgta aataaagatt cttccaattt atcattcgta ggaggataat
    27781 gagtaatcac aattattttt gagtgaccac ttttacgagc agattctaat gatagtctaa
    27841 gtctatgctc ttctctttta taaacttttt catcagtctc atcaaattta acatcattag
    27901 gacatatcca gcccctacct ccacaaattg cataatcttt atattcataa aaatttgtct
    27961 gaataaaacg catgtcttca tatagcttat ttaaacttgt aacggttgtc caccagtaat
    28021 catgatttcc ttttataagt attttctgac ctggaagttt gcttattata tctaaatctt
    28081 ttttagcttc cgataaatta attccccaag atgtatctcc tagtactaag accatatctt
    28141 cttccttaac aacttctttc caattatcta taattttctt ttcatgacca tcccaattac
    28201 ttccaaagat attcattggc ttattgactg atgttgaaaa atgtaaatca cctatagcat
    28261 acaaactcat aatatcattc ctttcttaat tacactaaat aggctgtctc tttttagaaa
    28321 ttattttcca ttaagaggca gccccattaa tatttagcaa attttctaca agtagttttt
    28381 tactgaacta ctttatcatc taccttagta tccttatatt gctctagtaa tatatctatt
    28441 tcttgtttta attccaaaat ttcagttata tttttatctg tttcagttct atcaagtttt
    28501 ctttggtact gcatagcttt atgtgtatca cccaagtcat ataactcttg tattctattt
    28561 agagtatctc taatagctat agacttcatt tggtacatag cctgagcttt cattatgtcc
    28621 tctcttattt cactcatttc ttggtctaaa agaaaggcag catcagatgc tcttctaagc
    28681 ctttcagcaa gagtctcagg tatgtaatgg tcatatttat attttaatga atgttctatt
    28741 gtagcccaga aattcattgc aagagttctt atttgtattt cacatataat ttcctttgaa
    28801 cccgcaatgg agtttatagg gtattttatt ataacatgat agcttctata accactatct
    28861 ttgtaatttg taatataatc tttttctcct attatagtca tatcatttct tacttttatc
    28921 aaatcaacta tagcatatat atcctcaaca aactggcaca ttattcttat accagcaata
    28981 tcatcaattt cagtttctat atcttttgca tttatccttt ttaacttaga tattatggaa
    29041 gatatttttt ttgttctgcc tgtgacaaac tctatgggag aatattcacc ttttgctaaa
    29101 tattcttttc ttatattttt aaattttatt ttcaattcct ctactgcatg ttcataaggt
    29161 gctagtatct cattccattt ttcgtattcc atattgctca ccctttattt gttttttatg
    29221 accttcttta ttttatcata atttattttg tttttgtata gtaaagcaat tctccatttt
    29281 ccaatatata ccctatttct tctaaatcaa ctaagtaact tatgacagat actaacgaag
    29341 atttaaaaaa atgatactct ttataattat tgcttaaatt attgttattt ataatgtttt
    29401 ttaataaaat ttctaaagat ttcggttcta caagcaactc tcttatttga tataaatatt
    29461 tttttaaagc attcaaatgt ttttctatta atttataaga atcttctttc gatataactt
    29521 gcttgccatg ccctaaaact aagtactcaa aatctatatt ttttaatttt tctaatgaat
    29581 ttatttgttc ttgtatatca tatagaaaca agaaatcgta ttttgataga atttcatctc
    29641 ctatcaataa atcgccaaca aaaatcacat tatccttcgt caaaacagct atactaccag
    29701 gtgtatgacc ctgaaactca attatatcaa acatttcatt gtttaattca atttttccaa
    29761 cttctataga tttatctatc tttatatcat ccaacatttt attttttaag atattatcca
    29821 taaatacatt gctctttccc ccaacaatat actttgaaaa caattctgga ttttctatat
    29881 ataatttgtc gtattcagat gacattatac acacatcttt ataatattct ttaaattgat
    29941 tacaagctcc atagtggtca tcatgaccat gagtatttat aatatatcta acctgtatat
    30001 tattttcatc taatacttta tttactctcc taggtcttga accacataac cctgggtcta
    30061 tcataagtgc tgtgttatct tcaaatacat atattcctgt attcgtgcca ccttttataa
    30121 aaaaggtatt tccttttact tttgtaagat tcaccttcct cacctcattt tgacaatatt
    30181 tatgatacaa tttattactt actaatataa aactatacaa gaacaattct ttttattcta
    30241 tctatagttc tttttcttat tttgtattct ttagatatcc ttattttcac attatatgct
    30301 aaatttctat aaagtaatga ctctataaaa tattcaacta tacctctatc cctaaagtct
    30361 atatctatat ttccacttga ataaatattt gagtctaata tcataagctc atcaaaaaag
    30421 cttaatctct tagatttact taaataatct ttatttggaa taaatctatc acaataatct
    30481 atcctgtaat ttactacttc atcatttatt tcaaatccat attgcttttt cattaactct
    30541 ctatagttat tatttttctc aataacatta tctaaatttt tattataatc ctcttttttc
    30601 caatgtccaa acatcctaca atttaatggt ctaacttcat atattagaca tctattatta
    30661 gtatctttaa atggacatgg gttctttctt gtatactcct caaaataata atctaatatt
    30721 ttacttatac agcttttttt taaatcaact ctatcttcaa gataacaaaa tatattcaaa
    30781 aactcaatta aatttatacc tactgactcc atacaacaat ttccacatcc agaacaattt
    30841 cctactggca aggacttata tacatcattt agtttgtcaa agagcttatt tttaatggaa
    30901 taatctatac aatttaaaat atctttattt tttatactag tcattattat tactccttgt
    30961 tataattatt aagttaatac aaacaattat tataacatat tttttaaatt taatcgcata
    31021 tggaggtatt ttatgaatag atatacaaaa ataattaata tgatgggaag ttattacaca
    31081 aaagactttg aaaaagaaaa aaagaatgta ataaaagtta gagaagttaa agaagataca
    31141 gttagaaaat ttttcttgca aggtgactgt gaagttcttg tagtatttga agacactggg
    31201 aaagaaatac ttattgatga cttttctcct gaagaagata tcaaaaaata tttaggaaca
    31261 aaatttataa ataaaaaaag atagttatca aaaataattc taaaattaaa atttaataaa
    31321 tgtacagtta taattctata taattatagt taaaagttat cttttattgc ttttttagac
    31381 tttaagatag ctttttttct attttttgta aaataattcc ataattcaca ttaataattc
    31441 acaaaatgat ataatataac aaatagataa attttaaatt tctaaataca taattaatat
    31501 tttgcgaggt gattttatgc aaatttattt tagagttaag gctgttggca aacgcaaacc
    31561 tatgttagaa cttacccctt ttgatgtatc tgacaacatc aattgcctaa aagaagctat
    31621 ctctgaaatt gtatctaaaa atgttcttga atataatgag aaaatcccag aaaaatcaat
    31681 aataaacttc ttaactaatg aagaaattga atctcaagct gatattggaa aagttggatt
    31741 tggctctatt tacaatgaga ataaacaatc tgtagaaaag gcaatagaag tagcgttaca
    31801 ggcatttgaa gatggaatct acaaagtgct tataaatgaa gatgttattg aaaaactcga
    31861 ttcccctata aatctgaaaa gtggagatat atttactttt ataagactta ccttgcttgc
    31921 tggaagaatg ttttaaaata aataatttgg aggtacataa atgttagaac gtactagagt
    31981 agaaaaaaat cctaaaataa ttgaaaattt taaaaataat tttactaaat cttcttcaaa
    32041 acagtactac gacttagcaa aatctctttg ggactgcata gaaaatagtt catactatta
    32101 tagagaacct gaggacaaat tcttaaatat gataaagaaa tactacaatt gccactacta
    32161 cgaaattaaa aatatggacg agctcctttc tggaccttta aaaaatgcat tagaattaat
    32221 atttacttct gaacaagtag aagaactttg ccaattaatt aaaagaaaga tggattatac
    32281 atattcagga ggatattatc gtaaagggta tcgctcaaat aaacttgtgg attattttag
    32341 aggtattata aggtttattt caaactatgt tgatttaatt tattatgatt tttcagttat
    32401 agattatctt actcttccaa ataaggtctt gtactatgac tctataatga gtgataaaat
    32461 agcactagaa atagacaaag gaaatcaaaa agtaacaagt gccttagaag aagtaatatt
    32521 tggagaaaat caaactgctc tacttacaag acaaatgata attggtatca tgaagagtca
    32581 tagtgataaa tgctatgaaa tgctttctaa acttcttttg gcagccaaac aacaagaagg
    32641 acttagacag caaattgtag agagtttaga tgagggtaca atttctgcat caatatacat
    32701 gttaaaaact attttggaca acaatttaga gcgatttagt tctgttataa gagccttgga
    32761 tacatggaca ggacttggtt tttcagacca aaaacctgga ataatacgta aatgtttaga
    32821 actttcatat aaatcattga ctgatgaaaa gttccgtata gaatcaatta atagcaatga
    32881 taatcttgaa atttattttg ccttatgggc tactgcaact tacaacatat atgactctta
    32941 ctctcttatt gaaaacttac tttcttgtca ggaaaaatat aaaaaacttg tagctcttta
    33001 ctttttatcg catacagaaa gtacatattt taaacattct atagcatcta aaaacattaa
    33061 gggtgatgat ttagagatac tagcatgggt aattacaaat ttctatgtag aaggttctgt
    33121 tggctactac tatagttggc aaaacagaga aactaatttt gacttgtcaa acactcctat
    33181 tgacaaagac tttgatgcta gaaaagaaca atttgaaagt ttaaaacata ctttagaaat
    33241 tttaaattca aagacaaaat cattttcagg aagtgtattt ccatggactt ggattgaaat
    33301 ttcttctcat gaaatactta aatgtatgat gactttaaca gcatacgata tgaatgctca
    33361 atttatagat gtgcttatgg aaaattgtca tcttatgagt tcagatttaa gagcttcact
    33421 tatagtcaat tttgtaaatg aaccaaagac agatagacaa cgtgaatttt tattggaatg
    33481 tttaagtgat aagagtgttt ccaatagaga aacggtattg agtttactta gaaagcttga
    33541 gctaacagat gtagaattag ataagataac caatctattg tctttaaaga caagctctat
    33601 tagacaaaca gtaataaaaa ttttacttga acaagatgat tcaaatttgg aaaatattgc
    33661 ttcttcactg ataaaatctt caaattctca aaaatgcctt ggaggtcttg aaattgtaaa
    33721 cttattaaaa gacaattctg atagacaaga actatataat aagcttattt tacttataga
    33781 ggatattgat aaatctaaaa agaatacatc tcaagttcaa atattaattg ataatttgtt
    33841 aggtaaagca aaaaatgatt actgcaaaga aaatggtttt ggaatatata accctgaaaa
    33901 caaagttcaa attccagaac aatttgattt taaagatgat tctaatagct catcaaagct
    33961 taataaaatt atttcaaata taagtttatc tactagtgag gattctgttt ctgtagcaac
    34021 aaaaaaagaa gcaaaaaaaa ttctcgatac ggatataaat aaaataataa aagtttatga
    34081 aaaatttgat gagcttatta ctgaaaatgc agattatgaa tatgaagttg aaagatggtc
    34141 aggtaatgta gacaaagtgc ttttaggtta ttctacttat aacttgactc ctattaaata
    34201 taaagtttct aatgatactc ttgataatta tccattggca gaactttgga gagaaactag
    34261 taaaaagctt gacttagatt acaaattaat acttgatatc ttattttata gtataagtac
    34321 atgtggatat aatgaaacag aaaaaagttg gttttcatct atatattcaa atgtccttga
    34381 ctctaaaaaa caaaataaaa tatatacaaa acttgaaaaa tttaaattta atggacttac
    34441 aagaaaactt ttaagagttt tactaaatga gtttgaccat aaagaaatat ttaactattg
    34501 tttagaaata tactataaac ttagaaactt attgactata gaacaatgta aaaaacaaag
    34561 agttgaattt actaaaaact attacagaca aaatgaatac tgccctgctt taaatgcccc
    34621 tattttaaac tattggcttg ataaagcaca ttcgtttatt tatgatgata tttcttttac
    34681 taagtttttc tacactacgt tttcaaatta tcagttgcta gaatataagt ttcctttcta
    34741 tactagcata aatgtgtttg ctcgtgcaca tgaattaggt ttaatgactg aagagcaagt
    34801 taatagagaa atgataagtg gatacaacgc atcaaatata ataagaacat taacaaatcc
    34861 tagatgggct ggtgacacat tagaaaagta cccttggctt aaagataatt taaaaactgt
    34921 tacaaaccga attatagaaa ttgaatcacg tagaggtgat atgccaacag aagtgtctca
    34981 tctagctaat aatatacaat actttgaagg tatagattat tttgtaaata ttttggtcgc
    35041 tcttggtaaa gaaactttcc agagaggata ttcatatggt agcaattatt caaaaaaaga
    35101 tgttttaagt tctctactta aaaattgcta tcctactaaa gaagatgatt caaaaaaatt
    35161 aaaaagttta cttaaaaata ccgacattac tgagttaaga ttaatagaat ctgctatgta
    35221 tgcccctcaa tgggtaaaca taattgaaaa atatttaaac tggaagggtc tgaaaagtac
    35281 aatttggtac ttccatgcac atataaatga aggcttctct gcacaaaaag aatcagaggt
    35341 tgctctattc tcccctattt caccacgtag tttcattgat ggtgcatttg atgtagattg
    35401 gttcttagat tcatataaaa ctattgggga agaaagattc aatatacttt acaagtcagc
    35461 taaatatata actagtggtg gtaactctca tagacgtgca caactctatg ctgatgctac
    35521 acttggtaag cttgataaag acatgttgat gaaagaaatt gaaacaaata gaaatcaaga
    35581 taaattgaga agtttctctt tacttcctat gaaaaaagga gacctaaaag aagcaacttt
    35641 ccgttatgaa tttattcaaa agtttgctaa agaaagcaag caatttggtg cacaacgtcg
    35701 tgaaagtgaa tcaaaagctt gtactatagc gctagaaaat ctagctagaa gtgctggatt
    35761 tggagatgta aatcgtatga tgtgggctct tgagactgaa aaactaaatg aagttgctca
    35821 tctatttaaa ccaaaagata ttgatggtac ttcaatttgg atagatgttg atgaaagcgg
    35881 aaaagcatct actaaagttc aaaaaggcaa caagtcttta aagagtatac caaaagctat
    35941 tgctaaacat ccatatgtac ttgaacttaa atctgtccaa aaagatttaa agagtcagta
    36001 ttctcgtgca aaacagagtt tagaacaagc tatgaaaaat gaaatagtat ttacagttaa
    36061 agaaattgcc aatatctata aaaatccagt tatctgccct ttgattaaaa atctagtatg
    36121 ggtatgtgat gataatattg gatatgttta taatgataag tctaagttct ttatttgcaa
    36181 tatagaaggt gaaacttata agttgacagc aaaaaataaa ttaaaacttg cacatccagt
    36241 tcatttgttt gaatctggta agtggagtga gttccaacat gatttattta gtaagcaact
    36301 tgttcaacca tttaagcaag tattccgtga gtattataga ataaattctg atgaaatatc
    36361 tgaaaaaact atatctcgca gatatgctgg tcaccaaatt cagccaaaga aaactgtagc
    36421 acttcttaga aatcagcttt ggactgtcga ttatgaagag ggattacaga aagtatttta
    36481 taaagaaaat atagtggcaa agatgtttgc acttgcagat tggttttcac cagcagatat
    36541 agagcctcct actattgaaa cagtagaatt ttttgataga aaaacttata aaagtttaga
    36601 attagcttct atacctccaa ttctattctc agaagttatg cgtgatatag accttgttgt
    36661 aagtgtcgct catgctggtg gtgttgaccc tgaaactagc cattctactg tagagatgcg
    36721 tatagcaatt gctaaagaac ttttattatt attaaaaatt aacaatatca attttgaagg
    36781 ctctcatgtt cttatcacag gtaatttagg tgagtattct gtgcatatgg gttctggtat
    36841 tgtacataaa aaagggatgg gtgctttgaa tatacttcct gtacattctc aaagtagagg
    36901 taagatattc cttccattcg ctgatgaaga ccctaagact gctgagatta tgtctaaaat
    36961 cttattactt gctgaagata ataagattaa agaccctagt atcttaagca acttataaat
    37021 ataagattta tctctaaaac atattgggct ttctaatttt ccatgaaagt ccaatatgtt
    37081 ttatattata ttttatacga attaatttat aactatagca aatttatatg atatcttcat
    37141 catacatatt gtatatcact gaaaattttt ttcttcatta aaacctttta accctataaa
    37201 atctgtatag aaaatctttt tct
//
LOCUS       DAEPOG010000017        37198 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEPOG010000017 DAEPOG010000000
VERSION     DAEPOG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.017"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(140..334)
                     /locus_tag="KR432_000756"
     CDS             complement(140..334)
                     /locus_tag="KR432_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639310.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
     gene            complement(643..2172)
                     /gene="lysS"
                     /locus_tag="KR432_000757"
     CDS             complement(643..2172)
                     /gene="lysS"
                     /locus_tag="KR432_000757"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH0639311.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2187..2666)
                     /gene="greA"
                     /locus_tag="KR432_000758"
     CDS             complement(2187..2666)
                     /gene="greA"
                     /locus_tag="KR432_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH0639312.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2775..3743)
                     /gene="dusB"
                     /locus_tag="KR432_000759"
     CDS             complement(2775..3743)
                     /gene="dusB"
                     /locus_tag="KR432_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH0639313.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3880..4650)
                     /locus_tag="KR432_000760"
     CDS             complement(3880..4650)
                     /locus_tag="KR432_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBH0639314.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4669..5259)
                     /locus_tag="KR432_000761"
     CDS             complement(4669..5259)
                     /locus_tag="KR432_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0639315.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5443..6411)
                     /locus_tag="KR432_000762"
     CDS             complement(5443..6411)
                     /locus_tag="KR432_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBH0639316.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6404..7381)
                     /locus_tag="KR432_000763"
     CDS             complement(6404..7381)
                     /locus_tag="KR432_000763"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBH0639317.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7695..9665)
                     /gene="ftsH"
                     /locus_tag="KR432_000764"
     CDS             complement(7695..9665)
                     /gene="ftsH"
                     /locus_tag="KR432_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0639318.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9699..11087)
                     /gene="tilS"
                     /locus_tag="KR432_000765"
     CDS             complement(9699..11087)
                     /gene="tilS"
                     /locus_tag="KR432_000765"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH0639319.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11270..11698)
                     /locus_tag="KR432_000766"
     CDS             complement(11270..11698)
                     /locus_tag="KR432_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBH0639320.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11715..12521)
                     /locus_tag="KR432_000767"
     CDS             complement(11715..12521)
                     /locus_tag="KR432_000767"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH0639321.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12723..13208)
                     /locus_tag="KR432_000768"
     CDS             complement(12723..13208)
                     /locus_tag="KR432_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBH0639322.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13259..13918)
                     /gene="spoIIR"
                     /locus_tag="KR432_000769"
     CDS             complement(13259..13918)
                     /gene="spoIIR"
                     /locus_tag="KR432_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBH0639323.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14063..14743)
                     /locus_tag="KR432_000770"
     CDS             complement(14063..14743)
                     /locus_tag="KR432_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639324.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14727..15617)
                     /locus_tag="KR432_000771"
     CDS             complement(14727..15617)
                     /locus_tag="KR432_000771"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH0639325.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15735..17285)
                     /locus_tag="KR432_000772"
     CDS             complement(15735..17285)
                     /locus_tag="KR432_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0639326.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17379..17645)
                     /locus_tag="KR432_000773"
     CDS             complement(17379..17645)
                     /locus_tag="KR432_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBH0639327.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17793..18653)
                     /gene="yabG"
                     /locus_tag="KR432_000774"
     CDS             complement(17793..18653)
                     /gene="yabG"
                     /locus_tag="KR432_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBH0639328.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18737..19675)
                     /locus_tag="KR432_000775"
     CDS             complement(18737..19675)
                     /locus_tag="KR432_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBH0639329.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19785..19946)
                     /locus_tag="KR432_000776"
     CDS             complement(19785..19946)
                     /locus_tag="KR432_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639330.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19956..20453)
                     /locus_tag="KR432_000777"
     CDS             complement(19956..20453)
                     /locus_tag="KR432_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0639331.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20568..21278)
                     /locus_tag="KR432_000778"
     CDS             complement(20568..21278)
                     /locus_tag="KR432_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBH0639332.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21454..21984)
                     /locus_tag="KR432_000779"
     CDS             complement(21454..21984)
                     /locus_tag="KR432_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639333.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(22001..22231)
                     /locus_tag="KR432_000780"
     CDS             complement(22001..22231)
                     /locus_tag="KR432_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639334.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22246..23640)
                     /locus_tag="KR432_000781"
     CDS             complement(22246..23640)
                     /locus_tag="KR432_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0639335.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23766..25019)
                     /locus_tag="KR432_000782"
     CDS             complement(23766..25019)
                     /locus_tag="KR432_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639336.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25070..26224)
                     /locus_tag="KR432_000783"
     CDS             complement(25070..26224)
                     /locus_tag="KR432_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0639337.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(26783..27352)
                     /locus_tag="KR432_000784"
     CDS             complement(26783..27352)
                     /locus_tag="KR432_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0639338.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27555..27782
                     /locus_tag="KR432_000785"
     CDS             27555..27782
                     /locus_tag="KR432_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0639339.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(28001..28744)
                     /locus_tag="KR432_000786"
     CDS             complement(28001..28744)
                     /locus_tag="KR432_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0639340.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28912..29784
                     /locus_tag="KR432_000787"
     CDS             28912..29784
                     /locus_tag="KR432_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0639341.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29784..30449
                     /locus_tag="KR432_000788"
     CDS             29784..30449
                     /locus_tag="KR432_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0639342.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30470..31327
                     /locus_tag="KR432_000789"
     CDS             30470..31327
                     /locus_tag="KR432_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0639343.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31483..34029)
                     /locus_tag="KR432_000790"
     CDS             complement(31483..34029)
                     /locus_tag="KR432_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0639344.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34029..34715)
                     /locus_tag="KR432_000791"
     CDS             complement(34029..34715)
                     /locus_tag="KR432_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639345.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34798..35787)
                     /locus_tag="KR432_000792"
     CDS             complement(34798..35787)
                     /locus_tag="KR432_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639346.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35774..36460)
                     /locus_tag="KR432_000793"
     CDS             complement(35774..36460)
                     /locus_tag="KR432_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639347.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36960..>37198)
                     /locus_tag="KR432_000794"
     CDS             complement(36960..>37198)
                     /locus_tag="KR432_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639348.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        11904 a         6883 c         4169 g        14242 t
ORIGIN      
        1 cttaaaaata tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt
       61 ggttttttta ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata
      121 catgcatata taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc
      181 tcactctatc tgcccaaata tttatactct taactttatt aacattagtt gtaccctcaa
      241 aaatatccat tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac
      301 aaaataaaca taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat
      361 atatagtatt ctcataaatt attcttttta ccttctcata aattattcta aatattttca
      421 taataaatta ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata
      481 tcaatttcta acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca
      541 tataaaaagc attctacact gacattttaa acaaaattta gctatttatt gctaatttaa
      601 tagctaaact ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt
      661 atttggattg ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg
      721 agagttagtt aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg
      781 tagacctacc tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc
      841 taattctctt tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag
      901 ttcagaaaat gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct
      961 tcttgggtct tcaacatttc tcttagataa tggagatact tcaactggat gatgagttat
     1021 aaatgttggt tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc
     1081 tcctcttctc attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc
     1141 ttcatctgta tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat
     1201 actcattctt ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat
     1261 acttcctgta gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc
     1321 agtatagtct gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa
     1381 atccatacct tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac
     1441 aataagtctt tttaaataca attcatttgc tattcttaaa tacattggta tatgtaaagt
     1501 attatgatta gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg
     1561 agtctcaact tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt
     1621 tgttctaatt aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg
     1681 tctatatctt agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga
     1741 tttacatagt aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa
     1801 gatttctcct tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc
     1861 ttcctctcct atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat
     1921 atctataaag ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt
     1981 agttttacct tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc
     2041 atatctactt tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct
     2101 tcttacttga agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg
     2161 attatttttc atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca
     2221 actccatcag gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc
     2281 cctacaggag attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact
     2341 atagtatatt caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt
     2401 gtaacaacat taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt
     2461 tctaatttta aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca
     2521 gcattttcag ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt
     2581 ctctttacaa cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc
     2641 aataaaaact ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa
     2701 ttttcctaat tatattttaa aaattttata atcttttttt aatttatgcg ctatattata
     2761 agtcactatt atttttatgt caaggagtct tccatatagt actccaattt cgacacaact
     2821 tcttgatagt cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct
     2881 tttaaatacc aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct
     2941 tctactgcaa gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct
     3001 ggcaaaatct cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct
     3061 tgagcacccc tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa
     3121 tcttctattg taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc
     3181 tctgcaatta tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt
     3241 attgctaaag cgcttattcc acaatcctct gctatctttg ctatttcaac agcatttaca
     3301 ctattatcat cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact
     3361 actgccctca atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca
     3421 tttttaacta cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa
     3481 tcattcatta tctctgctgc tcttcccata aactctggtt ctgaaccgaa tatctgaact
     3541 gctacaggat gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag
     3601 caaagtgcct taccatttat catttctgta tataaaagcc cacatccctg ttctttacaa
     3661 attaatctaa atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc
     3721 aattctaaat ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta
     3781 actttaaatt cttatttcct cattataata taccacatat agctagaaat ctatcataaa
     3841 aagggaattg gatatataac ccaattccct gtatattaat tattcttggt ttttttcata
     3901 tattattctc aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc
     3961 tgaagcaatc agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa
     4021 ttctttcttc atcatactta ttattttgtc aactaaacca acatatccat aaattattcc
     4081 agattgcatg gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac
     4141 tctaggtaat ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc
     4201 tattgttcca cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc
     4261 aaaatcaaca agtatacttg gtgctccata cttttctatc cctgctacag cattaactat
     4321 tctatctgcc ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc
     4381 tggacctact attaatggct gttttttaca gtactttata caaaagtttt caagagaatg
     4441 catgacattt ggaactacag atgatattat aacatcatca atatcactta tattcacatt
     4501 atcataatca aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc
     4561 tgttttaatt ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt
     4621 agtatttcca acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct
     4681 tttcttaagt acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac
     4741 cccatttgct gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc
     4801 atgaagatac ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa
     4861 cagagaacct attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat
     4921 tattaactga tatacataat aagctaatac acctatcata actcttggta atattgaaac
     4981 taatggattt ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact
     5041 tgatatccca aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc
     5101 aataataact ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa
     5161 tggagtcata gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct
     5221 tacatttact tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca
     5281 gctcattaag atgccggact acttaatttt ttaatttttt tcgttttttt cttataacta
     5341 tgagtcaagc taccattgta ggatgcccgc tctaacaaga ataattttat caagatgagt
     5401 tgccattgtc aacaatttga caagaaaaat atgctaattt catcatattt ctcgtttttt
     5461 tactatatct ttatatgcca ttatattttc tacactatcc gcttttttta ctgcatttat
     5521 tatacttttt tccacaacct ctacagccaa tgaacctact aatgttatat ctgcttctat
     5581 ttctcctgtt gatattgtga atattgtatc tccatcatga ggtgtatgga taggaaaaat
     5641 agacttagca tatccatcat gtcccatttg agatatcttt ttacatccag ctttggttaa
     5701 cttagcattt gttgcaatta tcccaattgt agtattatct acactaaatc cacctttact
     5761 ttcaccttgt ttcattaatt tatatgtatc aattgtttct gtctttttat catttaaagc
     5821 accagctata accttaccat tttcataaac atctccaaga gcatttacag caattaatgc
     5881 agatacaatt agaccattat ctaattttat agaatagcta cctattccgc ctttcatagc
     5941 atattcctga cctcttattt ttccaacagt ggctccacaa cctgctccat aatttccttg
     6001 atttaaaata tcagtatttg caacttcaca agctctataa cccatcttta aatctggtct
     6061 acatttaaag ctccctactc ctaaatcaaa taaaactgct cctacaacta taggcacttt
     6121 acctacacct acatcaaagc caattccttt ttcttctaag tatctactta caccacaagt
     6181 ggattcaaga ccatatgcag aacctccagc caaaactact gcatgaactt tttgaaccat
     6241 atttacaggg tctaataaat cggtttctct agtccctgga gcagaacccc ttacatcaac
     6301 accgcaagta gctccttctt cacatattac tactgtgcac ccagtaagac catttctatc
     6361 ctcaacctgc ccaaccttta agccttctat atccaaaata ttattataca tatccttttt
     6421 ctcctcttat agaaacttca cctgaaataa tttcttttat acttccatca cttctttgaa
     6481 ctaataaatt tccctcttca tttatgtcta aacatttcac tagttctcta ttatctcctt
     6541 ttaatacata tatattttta tctataattg cagaataatc tctacatatt tttaaggttt
     6601 tttctttgga attatttaca gtataatcta aatataattt ttcaaattca gtcaatacat
     6661 tcctaacaat atctactctg gataacatat acccttcttt atacaatgat gtggctttgt
     6721 cagctatttc ttgttcaaaa tccattattt taacatttat tccaattcca agaacaacat
     6781 aatttgttct ttcaatttca gcagataact ctgttaatat accacatatt ttttttccat
     6841 ttaaaataac atcatttggc cattttatta atgtatttgc tcctagattg tttaaagctt
     6901 tcgttacact tgctcctgct attaaagtta taaatggagc tttgaatggt tccatatctg
     6961 gttttaatat aatactcatc cagattcctt catgagtttt tgaatcccat cttcttccca
     7021 atcgtccttt ccccatatct tgatgttcac ttaaaatcac agtcccatct acactattta
     7081 tagctatcct tttagcatgt tcatttgttg aatctatagt gtcaagatgt tcaatatttt
     7141 ttccaataaa ctctgtatct aatttatagt aaatattctg tgggcttaat aaatcagttg
     7201 gattttccgc taatcgatac ccttttttat ttacagattc tatattatat ccctcttctt
     7261 ttaaggcttt catatgtttc catatagctg cacgtgacac ccctaattgt tttgataatt
     7321 cttctcctga aataaattca gatttattat ttaatataat ctctattatt ttatctcgca
     7381 tatttcacct ctaatatgac ataatttcat gacttatttt cttctaataa ataattatac
     7441 attattaata tacttttaat actattaatt ttaaatttcc tttttaatta ttataatggt
     7501 tgttttatat tttatattga tgatttatat aattttacat tttatttaaa taattttaaa
     7561 ataataaaaa actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa
     7621 tttaatatta tactttcagt tttagtattt aatccacatc agataataat cttaaaatga
     7681 ccaaggtctt aaaactattt atctttatca gatttatctt ctaaattttt atttatatca
     7741 ataacattgt ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact
     7801 tctttagaag aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta
     7861 ttaaatgctt ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct
     7921 acatactcta atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca
     7981 tcaactatac gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga gtaatttctc
     8041 ttacttgaaa aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca
     8101 agtttagaac tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct
     8161 aagtcatttg aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca
     8221 ccaagtaaaa ctacaatatt ttctttcatt tcattttttg tagcatagaa cttatcttca
     8281 acaggaagtt gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga
     8341 acaggactta cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct
     8401 gtaagtcttc tttctttctc acttattact ttcgattttt tagccacacc tgctataacc
     8461 tttgttatag cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt
     8521 aatattgcag cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt
     8581 ctagccaaga catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta
     8641 aatatagcct ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt
     8701 cctggtctaa gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt
     8761 ataccttgat ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct
     8821 ctttcatcat gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca
     8881 tctatgaata tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt
     8941 gaagcaccaa ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga
     9001 actcctgctt ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct
     9061 accataagca ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt
     9121 aagaaatcta ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta
     9181 aatgtaactc tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc
     9241 ataacctttc cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg
     9301 aagaatatta acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct
     9361 tcaccaccaa aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta
     9421 tttcccatca cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta
     9481 ccttctacag aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct
     9541 ctatatactt cagagaattt taaatctttt attttttctg taggcttacc tgagaattga
     9601 actataccta ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta
     9661 ttcaatatag aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt
     9721 tttaatactt cttttgtgtt ttcatcaatt ttataatttt cactgatttt ataatctcca
     9781 acacatatta ttccttcact atctgtgatt ataggaattt tacatctttc ttctcttggg
     9841 atttttaaat ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta
     9901 tcaccttgcc ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct
     9961 ttagatgaat tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca
    10021 ataatttgtt ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat
    10081 tcaatttcct cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa
    10141 acaaatatat ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac
    10201 attatatctt ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg
    10261 gaatttctaa gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt
    10321 atctctacaa agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct
    10381 ttctcaatat aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt
    10441 acatttgaat taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta
    10501 gtgtatatat tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa
    10561 tactcctcta tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta
    10621 atataatcaa ttccctttaa gccttttaag cctgttcctc tcatgatacg catcataaca
    10681 gtttcggctt ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc
    10741 ttaatttcat aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc
    10801 tcattttcac aatattttgg tacattaata gactttacaa agaatataat tcccatatcc
    10861 tcacaaagtt tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg
    10921 tttaaatgtg ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc
    10981 aataaacaaa ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt
    11041 attaaattat gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt
    11101 acacttagtt ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat
    11161 caattttaag aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag
    11221 gtagtttttt cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac
    11281 ctatgtatat atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt
    11341 ctattttcaa ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa
    11401 tatcaattaa taaagtacct tgtggggtca catactttgt agtactactt tctccacatt
    11461 taaacagcat tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta
    11521 tctttatagt attagttatt tctatatttt cttctttttc cttgtaaata acatatattc
    11581 catcattttt ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt
    11641 tttcatcata ttgtctagta tttatactta tttttacact caacgatttt tcctccatag
    11701 attcttcata taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc
    11761 tttaagaact ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga
    11821 taagtcccat gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa
    11881 tcctttgcag tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct
    11941 attgttctct ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt
    12001 tctttcaatt cttgtaaata tatctttgct gtcaatttag ccacttctgt atttgcccat
    12061 ccttcttcaa caataggtac aaataaagga catgctttat taacaatttc tatactctca
    12121 tctattttag atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt
    12181 attcctacaa ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact
    12241 ccaattatcg gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg
    12301 ttacatgcta taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa
    12361 gtatatttaa ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca
    12421 aagtatatta tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca
    12481 cctaatcccg aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt
    12541 tacatttttg tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat
    12601 taatatatta ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta
    12661 tcttaaatta gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa
    12721 atttattttg caaacacatg atttcctatt ctcatataag gattcaatct atttatccat
    12781 ttgcaagtgg ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca
    12841 taaagagctt cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca
    12901 tcaagcacaa ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat
    12961 ctagggtcca atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt
    13021 tctcctctag cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa
    13081 tttattactt ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct
    13141 gttggttcat atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt
    13201 gtagccatca gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt
    13261 aaaatttttt ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac
    13321 ttgttttttt tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct
    13381 tcaacttatc atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg
    13441 gagggaacat aacacaccac cagttttttc cttcaccttt cccaatgata atctttaatg
    13501 ctttatattc accagctgga agaactatat tagaatattg tttagtaggg aaattgctat
    13561 attgtatacc tacctttaca ctgtaattat atccattttt aagaataata tttttactta
    13621 tttcttccag actactatat tcctttttta taatagcttc actttctttt atactttttg
    13681 aattttgaag ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt
    13741 cttgatcttc atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat
    13801 aatcttttga tatattgtct acttttttta cctcaccatt tattactatt gtcattattg
    13861 atatgactga tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg
    13921 aaaattcccc ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt
    13981 cataatattt tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa
    14041 atagcttttt aaaccttata ttttataagt ctttactaat catagcctta tctatcataa
    14101 actgctctgc tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt
    14161 taattatagc atcaagtaag tatttatgct cttcaacaat attatgtgat gtatcataag
    14221 cagacatata ggtaactcta aatctatata cttgttccca taatgagttt attagttgga
    14281 ttaatttttt attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta
    14341 ataacgcatc tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa
    14401 gctttttaat atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta
    14461 ggcttgctct tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg
    14521 caccttttct aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc
    14581 tcactggagt cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac
    14641 caggctttaa ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta
    14701 atggcttgta attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt
    14761 tgactgctat taacaacata cacctgttta tattttttta ataattcttt ttttccaatt
    14821 agagcatctt ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata
    14881 gaacctaaag cattattttt catcattact tgttttatat cacttatttc tttgtgttta
    14941 cctatagtaa cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca
    15001 gattttaaac actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct
    15061 aatccttgat aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt
    15121 gtatcacatg gtaaaccttt gatgtttgtt agcttttctc caataccttg agctaaggca
    15181 ggtcttcctg atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat
    15241 tcatcttcag ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca
    15301 ttagaacttc ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct
    15361 acaccttttt ttatataaaa tttattttta agtattttgg cagctttata aacaatatta
    15421 tcttcatcta aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat
    15481 tcttttatct ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga
    15541 taaccgtctt gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt
    15601 agtcttataa aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat
    15661 attatactac aaaaaataaa ataatagggt attataatac cctattatac ttaattactt
    15721 taaatttttt aatattaatc tactaatacg actttttttt ctaaaataag acacttacca
    15781 ggcttaattg gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta
    15841 ttgtattttt ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg
    15901 ctaggagctt tagataaatc atatacacct tggtcttctc ctttgataat aaaattttct
    15961 gctttcttat ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc
    16021 ctattcaaat caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca
    16081 gttagattat ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc
    16141 aattttaaac cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg
    16201 cttttatttc cttcaatata actattttct atagacatgg ttggacaaac gcttacaata
    16261 tcttttattt gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct
    16321 tcactacgtc ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt
    16381 tgaggcgaat atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta
    16441 caacaagcca tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa
    16501 atatgattaa aatctgacat agacaacaat acttcttcag tcataccatc actaactcca
    16561 gggacttcta caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc
    16621 tcatatgtac aagctaacgg atttatttct aatactccac caataataac tttattgtca
    16681 gtcactctac tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc
    16741 tctgtgttta ttgttatagt atctcttata gaactttctg ctttttctat tccaactata
    16801 tcttgaaaac aaatttcctt tctgtgcttt tgtatgcctt ccacttgcgc cacatctttt
    16861 acaatgtcta acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact
    16921 ttaatctttc tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg
    16981 tattccatat cttgaacaac attgtctttt tctattactt cgtttatatc tacttttccg
    17041 tctacattag aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta
    17101 cctctgcaaa gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct
    17161 gtttttacaa tttcatatac atctaatttt ttgtctggta caactgcctc cgcttcaatg
    17221 aatgtttgaa attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat
    17281 tccatagtct acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc
    17341 aaaaaaaaaa ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct
    17401 tgatctctaa aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat
    17461 actctaggat atccattact ttcatcatct aactttataa caaacacgct tggataaact
    17521 ttttctagta taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat
    17581 attttctttc ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta
    17641 gccacaatat caccttcttt aacgtttata tgtttacata atatcacata taaacatttt
    17701 tgtcaaatgt ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt
    17761 gaagatatac agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc
    17821 catctaaatt ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc
    17881 tctataacct catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact
    17941 ataaaaacag ggtctaatgc atgtatcatt attcttccag gcgaacttgc ataatttgct
    18001 cctgcactta ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta
    18061 tccaaatttg gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt
    18121 ctatagttac tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt
    18181 attactagta tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt
    18241 tgctctgaaa cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa
    18301 atttttaaat attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg
    18361 ttaggattta attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct
    18421 tttcttactg acttatataa taaattttct acatttttat ctattaatat atcttttata
    18481 tcaggtgctt ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca
    18541 attcccttta gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta
    18601 aatactatat ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa
    18661 cctccaataa atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat
    18721 gtcaaaaaaa catttcttat aatacttctt ttatcactct taaagcattt ttatagtata
    18781 tcttttctat atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca
    18841 tctgagatat atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctaggg
    18901 atacaacatc tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa
    18961 gtttactttc acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac
    19021 ctttatttgc taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg
    19081 ctcttgaatt agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa
    19141 atccaccatc tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta
    19201 ccacttctct tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa
    19261 agctcaattc attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa
    19321 atttctttaa attttctaat ttaccttcaa gtactgcacc ttcttcaata gataaaagag
    19381 ctgtaagttt accttctgat tggtttttca taatttcttc atagttagta gctaaagcta
    19441 tttcatcact attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat
    19501 caaaaggatg ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat
    19561 cccccttttt caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt
    19621 ctacattttc cattaatttt gctatagtat cacaatgtaa atcaataaat ttcatattta
    19681 taccccctgt atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac
    19741 tattgacaac tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag
    19801 ttaggtatta cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca
    19861 ctaatatatt tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca
    19921 tcatctgcaa atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat
    19981 ttttttccgc ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt
    20041 gttttagact tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct
    20101 tcccaacctt gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat
    20161 ttttcccaat ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata
    20221 tcttctttta agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt
    20281 ccaaattttt cagccaattc acttacttta ccttcaacaa catccatttt attatttaat
    20341 atttcattgt atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct
    20401 tcttgacttt catgattatc acttaaattt ctccattcag tatataaact catacttttt
    20461 cctcctaagt atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg
    20521 gaagtttctt ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca
    20581 taattaaaca attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt
    20641 gtcgcaattg ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt
    20701 tcaaatggta tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac
    20761 tctgattcta cttctaaatc aaatccatac agactatcta ttattattaa ttctgaaaat
    20821 atgttatatt ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatac
    20881 ctaaatacat cttttagatt atttacatct acatcatagt tctcaggctc tctagtatcc
    20941 ttatgttggt ctacatgcac taacttgcat ccttttgtaa attcaccctt taacaaagat
    21001 tttgcccaaa aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc
    21061 aggacatatt cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact
    21121 tcattaaata ccacctcatt gcctaacctt acatcattta atgtaccatt tataagtttt
    21181 ggaacgtata tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt
    21241 ttttctatat aaaatccact atattcataa ttttccataa aactttattt cctccattgt
    21301 tatataaata tataagttat aattttaaaa attaaaatgc acttattgta aatcatatta
    21361 tctattatag aaaacatctt atataactaa atataatatc ttctctcaat atgtattata
    21421 caataagtgc aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc
    21481 ttcaattgga aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca
    21541 atatggtcta aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa
    21601 cctatgaatt aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt
    21661 attactatac tgcacaatat tacaatccac attttttaca atttcatatg taggaaaatc
    21721 ttgtactata ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc
    21781 attagaatat ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat
    21841 tttaaactct atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt
    21901 actaaataaa gaatttttcc cacttttagc tatctctata gcttcttctt ttgtaattct
    21961 tataggtaac gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt
    22021 ttaaaactct atatatataa tcaaaaaata ttacaattac aactgccata actccatatg
    22081 gaattatttt tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg
    22141 ttattaaaaa gtacaataat actagaaaac cgtctactcc aagccatttt atttctaaat
    22201 ctttttttac cttagacact aatttagaca tagcagtttc tccttttata gattattttg
    22261 tgtatttaaa ttttcaccta ttctattacc aatcatccat gcaataaatg aaaaaggtat
    22321 agctatcaca agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa
    22381 agtcactcca cctacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc
    22441 aagaagccca cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat
    22501 acatgcatca tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa
    22561 acatacacct attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat
    22621 tcttccatat atatcatttg ctaatgtagc tccagctgct agataatagc tatccaatgt
    22681 tgatattata gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt
    22741 atctaaaact aaagttatat atccagcggc tggagccatc tctggataag tagctcttac
    22801 tataagtcct gttatagtta acaccatgtc aaaacatatc catagtgaaa aacaagttaa
    22861 taaggctctt cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg
    22921 gtctgaataa gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc
    22981 ttttgtaggg tgcataagct gtggtgcatc tttgagtgct tctgtgagac cttgaaatcc
    23041 tcctatttgt tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa
    23101 caataccata tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac
    23161 tactcctacc attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat
    23221 agttcccata gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc
    23281 aattatagca acatgcttac cataaaaact ttccagtaaa tcaggaactg ttatattggt
    23341 tcttatatga atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc
    23401 tattgaccaa actgcccatg cagatattcc atatatagcc gcatactcta cactacctac
    23461 tgtaccaact cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa
    23521 acttcgattt cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc
    23581 tattcctaat aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat
    23641 atctaatctc cacattctta tattttaatt tttaacttta atatctaaat atgaacaact
    23701 ctacccattt ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct
    23761 tgttattaag caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa
    23821 gcataagtag caaatatccc tataactgca catactacag ataaaataaa tatatatgta
    23881 tatcctgttt gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct
    23941 ggagaaaatc ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata
    24001 ttagactctg aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt
    24061 attatcatgt ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa
    24121 gcagctgtac ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca
    24181 actttatctg ttataacacc agcaactgga gatgctccca tttttataac ataagttctt
    24241 attattgata aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga
    24301 cttaaataag ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac
    24361 caagcctttg gcattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat
    24421 gctccactat cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat
    24481 gctgcagaat ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca
    24541 aagaagtaaa gacctataaa agaaattaac gtacctgata cacctgataa cccttcataa
    24601 aaaccaaata ttcttccttg ttctgactca cttccttgca ttctaacaac cttaacagat
    24661 gctgagaaga aagttaatat tgttgtaaat ccccataata caaatattaa gagtatcata
    24721 ctatatgatg ggaatgtaga gaaataaaaa ccaagtactc ctgaagcaat taaagaaaaa
    24781 gttatcagct ttctaaccga gaatctatct gcaaaccatc ctcctataaa ataagatacc
    24841 attcctacta atccatataa actcaataag ttccctattt gtgtatggct caatgataaa
    24901 gcttgttgca ttgggtcata aaatgatgat ttcatatatg gtaaactata catagccgtt
    24961 gttccaaatg ccaatatgaa tattatcaag aatcttttaa aattactttt gttctccatt
    25021 aattttctct ccttaaaatt ttattgttta taatctcatt catgtaattt tattttaatt
    25081 tttttattgc ttgtttaaag tcatttatta tatcatttga agattctaat cctacagcta
    25141 ttcttattag attttctcca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg
    25201 ctattgttgc aagctctaaa gagttcatta attttctact agcttggtct cttgtcaatc
    25261 cattgatacc atccttaagt ttaaatgaaa ctacacctcc acctaaacca ttcatttgag
    25321 ttttacaaag ctcatattga ggatgagatt ttaatgctgg atactttacc aattccacat
    25381 tctcttcttc ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc
    25441 tcatatctaa cgttttcata cctcttaaaa tcatccatgc actcattggt ggtaaagatg
    25501 ctcctgtaaa acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg
    25561 ctactgctcc ccctaaagta tccccatgtc catttataaa cttagtaaga ctatgtatta
    25621 ctaaatctgc acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa
    25681 ctatagctaa gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt
    25741 ctaatgatgg atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt
    25801 cttttacatt ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata
    25861 acaactcaga catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt
    25921 caccactttt taccaatgct aatatagtag atgttattgc ccccatccct gatgtactag
    25981 atatggcact ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag
    26041 gattacactc tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta
    26101 ctgtctttgc agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg
    26161 ggtctgcact atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac
    26221 tcattttgat ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttaat
    26281 caatttagct tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct
    26341 aaaaatatga cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat
    26401 agagtcaata tgtttttgat atttttttta caatgtttaa aatagtcagt acaaaaatag
    26461 gtatatctta gaataactaa agtcatccta agatatacct atttttatat cacaatataa
    26521 ataattacat ataagcatta gtttttttat cataaataga tttttcaaga tttatcatct
    26581 tttcataaat atattatagt taaatatttt aatattacct aacatctaca ataaaatata
    26641 ctcttaaaat aaaattatat aattaaaaat actctataaa agctaaaaag ttttctattc
    26701 gctacatcta aaaataatta gatagaggta taaatgtatt tagtttattt atacctctag
    26761 tcttttataa attatatctc tactaaattg attttttagc acgaataagt gtgctcttgg
    26821 agataccagt catcttcaca acctctctat atggcttaga atttaataac cccaatgcat
    26881 attgtatctg ttgattgctg tatttctttg gcctaccatc tttaaagtcc tttttctttt
    26941 tagcaaattc ttttcctaat tgagtacgtt ctattatcat atttctctca agctctgcaa
    27001 ctgcaagtaa tgtagtcaaa aagaatttac ccattgaagt attttctaaa agacctatat
    27061 ttaaaatatg aacacttact tctttagcaa ataaaccttc tattagttgt atcccttctg
    27121 atgtacttct agctagtcta tccagcttag ttgcaacaag taagtctccc ttttttagag
    27181 atgatataat tttttcaaat ttaggcctgt ttgtagtagt gcctgtataa gtttccttta
    27241 ttattttagc atcattatat ttattcaaaa tttcttgctg ttgctgatac aaactgttgt
    27301 tgtctgtttg attttttgta cttacacggc aatatccata tataataatc atcacttcct
    27361 aacataaaga ttttgtacag aagttaccca gaagcttgtt tttacttatc tttatccatt
    27421 ttactaaaac gatttttggg tgtcaatact gttaactttt gacaccataa taattccttt
    27481 ataatttgtt tgactatgtc aaaaattacc aatataatga aattaatgag tttatacaaa
    27541 ttggaggaaa taatatgaat agtctgttaa aagaagcaat agatgaatta aaaaacatcc
    27601 attgcgggga agttttttta ttaaaagaat tgttcaaagg ctatgaatgg aacaggttgg
    27661 agatgaatat tagattgaaa ttaggaaaac tttttttgta tgaagtggta actaatccca
    27721 atttaaatgt agaggtcatc aataaaaatt ctgcttatca gcaaatgtat aaaaaaaaat
    27781 agtgacttaa atgtatataa tagtataatc ttcttattga ttcaaaagtt aaaactttta
    27841 atacaaaaca atcattctat tttaacattt atcaatatgt ctaaatttat ataattaaca
    27901 catacgaata gtattgttta aatcacatta tttaagatag ataaatctta accctactcc
    27961 agcataaata ctaaaatagg gcttagaatt atctatattt ttatcccata aaatgcttag
    28021 ctcttcctct tgattgaaca tatatatctg gggaaataca atagaagcgt aaatttataa
    28081 aatttccaaa gtcagaaaac ttcagttcaa acttaggtat accaaaactt tcaggtgcta
    28141 tttcatcaac gccaaaatag atgataattc cattatctgt aatataaaat gcttggtctt
    28201 caggtataac taactcaaca ttagaataat acatttctct attttcattt atttgagatt
    28261 taatgtaatc agtaattata ttaatataat caactccatc tctaaaaatg tcattaagta
    28321 aaagttcttt tccagttgtc aaatctatat tgtaattatt caatgtattg tatttaggcc
    28381 catagttatt agcaaacccc ataaggttta atatcatact cagtacataa tttttattaa
    28441 aagtaactgc gtaactggta gatacccaat agctttgttt tggaagtgaa ttttgagaag
    28501 ctgcgttatt atattctcgt tcttcttcct gtaagccatt tttaaaatta tttacagtca
    28561 ttcttacagc attgtttatg ttatttaaaa taactgggtt tacatacgtg gattgatttc
    28621 taatattgta tataacagga tactttacat catatgaaaa atacttactt ctttcaaaat
    28681 cttcattaaa ttgtacatat aatatattat atgtacaggt atttgtagat tgataatttt
    28741 ccattaaaat cctccccctt ttataaaaaa atcagataca atataatatg cttaaaacag
    28801 taataatgtt aatagtaaat gtaaagaata gaaagtatct aaaacagcaa aaaatgacta
    28861 attttgtggt ataatacata ggattgagag ttattttata ggaggaaaaa tatgttatta
    28921 ttagcagata aatggaaaga ttatgaactt atagatatgg gaaatggaga aaaattagaa
    28981 cgttggggaa gttatgtact tagaagacca gacccacagg ttgtatggcc aatggaatct
    29041 gaatgggctt tatggaaaaa ccctcatgga cattatcaca gaagcaataa aggtggaggt
    29101 caatgggaac acaaaaaaag atatccagaa caatggacta taaattataa aaacttaaag
    29161 tttcacatta aaccaactgg ctttaaacat actggattat tcccagagca agcagctaac
    29221 tgggattgga tgatagagaa aataaagaaa gctaacagac ctataaaagt gttgaatctt
    29281 tttgcatata caggtggagc tactgtagca tgtgcatctg ctggtgcaga agtatgtcat
    29341 gttgatgctg caaaaggtat ggttaattgg gcaaaagaaa atatagaatt atcaggtctt
    29401 aaaaatcaaa cagtaagatt tatagtggat gatgttgtaa agtttgttga aagagaaata
    29461 agaagaggta aaaaatatga tgcaataata atggaccctc cttcttatgg aagaggtcct
    29521 aaaggagaag tatggcaaat agaagaaaag ttatacagtt ttgtagattt atgtatggga
    29581 gttttatcag ataacccttt atttttctta attaattctt atacaacagg attttctcca
    29641 atagttcttg aaaatgtatt gtctacaaca ataggtaaaa agacaaatac aggagatata
    29701 tacggtgggg aattaggaat accagcttcc agagatggta agatacttcc ttgtggtata
    29761 tttggaagat gggagaaaaa ataatgataa aagtcatata tgaagataac catttattag
    29821 ttgtagaaaa accagttaac atattgtctc aaggagataa tactaatgat aaagatatgg
    29881 taaatctttt aaaagactat attaaaaaaa agtataacaa acctggaaat gtatatattg
    29941 gtttaataca tagattggat agaccagtag gtggagttat ggtgtttgca aaaacatcaa
    30001 aagcagcatc tagattgtct gagcaggtaa gaagtaagac tttcaaaaaa acttatctag
    30061 ctgttgttca tggcaagata aaatctaact cagatatact aaaagatttt ttatataaaa
    30121 ataaaaaaac taatatggta actgtagtga aaaaaagcca taaagatgcc aaagaagctg
    30181 aacttaacta taatgtatta gattttaaag aaaatctaag tttagttcaa gttaatttaa
    30241 agactggtcg ttctcatcaa ataagagttc aatttgcaag cagaaaacat ccattatacg
    30301 gagaccaaag atatggcaaa gaaataaata agattggaca acaaatatct ctttggtcca
    30361 ataagataga aatttgccat cctactacaa aagaaaaaat ggaatttaca tgtcatccac
    30421 ctgacaaata tccatggaat atattttaaa tctcaaatga ggtgattata tgagtaaaaa
    30481 attttcaata ggagaaatgt ctaaacttca taaaataagc atacagacac taaggtacta
    30541 tgaccaaata gatttattta aacctgttca tgttgaccaa gaaagcaatt acagatatta
    30601 ctcaattgac caattttcac aattagatat tataaaatat ttaaaatatt taggtatgcc
    30661 tttaaaagaa attaagtcaa agcttgatga aaataatgaa gaaattctaa aattactaac
    30721 aaataaaatt aatatagtgg atgaaaaaat taaagaatta gagcttataa agaaggtact
    30781 aagcacaaag aaagaaacta tggaaaaagg tatgaaagta gatgaagttg gtaaaatagt
    30841 gagaaaacat attccacgaa gaagtatctt atctatagag tatgaagaag gattaagtag
    30901 tgaagaaact atggaacttt ccagaagaca aatagcaaat atattagaag ataatatatc
    30961 tgtgttctat ggtggagtaa gtggacttgt atctatagat aaatttttaa aagaagataa
    31021 agttatttat gacaatagtt ttgttactgt tgaaagagac ttatttgata taaaatcaca
    31081 aaaacatata acagaaattt catctggtga ttttatttgt atgacatata aagggtctta
    31141 caaagaaaat tataccgctt ttaagaaact ggttgattat gttgaatata aaagaatacc
    31201 tattgaaaat tatatatatg aaatacctat aatagaccct ctgtctacaa acaacgaaga
    31261 ggaacttcta acagaaattc aaatacaagt taaaaagaga gaaattgact taagatgtct
    31321 agtgtagtaa tctagtttac ttacacatct taatatagtt gtctattatc aacatatgat
    31381 ttttctagat agtgattcct aacagtgaga ttttcaaaaa ataggaggaa atatggctaa
    31441 tgctaattcc ctcctatttt taattaatcc atatcatttt aattaagcca tgtcatcacg
    31501 aagtgcatca ataatatttt ctttttttat cttattaata gtatacaaca ttgtaataaa
    31561 tactataaat aaaacactaa atatactaat tccaatacta aaccatggaa tcacaaaatc
    31621 aatatcatct gtaaccatta ctttatgaat tagccatgaa gaaattactg ctataggaag
    31681 tccaaagaac aatgctctca tgccataaaa agcacactca aaattcatca tcttttggaa
    31741 atcatgctca gacattccta cagagcgaag catggcaagt tctctacgac gtaacttaat
    31801 attagttgaa attgtattaa acacattggc aatcgaaatc agagaaatca taattatgaa
    31861 agtataggca aaaacattgg caataaaaat gtaattacgg ttctcatcca tcattccata
    31921 catattgtac agattgtact cattagtaac tccctcactc tcaataattt gctccatttc
    31981 tgcaactgac tgagtaggat ttttcgattg aaagcttaaa ctttcaattc ctgtagaaac
    32041 accaggaatc tcaaacttat ctttggctga ataaggagcc aatactctga agcttgcttt
    32101 cttcacatct gaacttctga ttcctggagg agaatcaggt ggcactactt tgacaaatga
    32161 aatattcaca ttttgtctct caggtatctc caacttgcca ttagtttcag gagtgagact
    32221 aacattcata gaagaactgt tgaaaatatc aataatatta ttatcctttg ttaccataga
    32281 aacagcaatc agttttgcat tttgtccagt atattcttct gctggtaatc caagactttt
    32341 aataatattc agataagttc tatcatcaag aaattgcata tctactggta attcaagcat
    32401 ttcactcata gaatgtgaac cctcatagtt caaataatcg tctgtaagct cacttacttt
    32461 ggcaacacat gaataggtca taagtttctg atacaaacct tcgtaaacac catttgcagt
    32521 ttttagctta tcgtaaagct tcaacatttc gttgtctttc ataccttgtg tagaaaaaat
    32581 aatatcctga tcagtaacct ctgacctctg atttgacatc tgttttaggt ctgctactaa
    32641 agcacttgta gatataaata aaactatgct taaaacaagc gacaatacaa tacttcgata
    32701 acgtttcttg tttcttttaa agtttttcat agcaagaact ccctcaagac cataaactcg
    32761 ctgtgttagt tttgatgtct tcacagattt agattcaatt tttacctcat ttgtctgacg
    32821 aatacactcc atcactggtg tgttggcagc ctttcttgct ggtatgtaag cagaaattaa
    32881 gattgtaacc atacttacca ctgctgcaac gatgattgca gggatagaca atgttaatgt
    32941 caacggaacg tttacataga gaatatttgc aaagttttta gctatgacag aaatcacaag
    33001 tccaatactg actataccga ttgcaacacc aattggtata ccaatcgcac caatgcaaat
    33061 tccttcaaat agcaccgaat ttcgtaactg ttttgctgta gctccaacag atgaaagtat
    33121 tccaaattga tgtgttcgct cgttcaatga catgttaaat gaattatata ttagaaaaat
    33181 tgagcctagc atgattatgg caagcacaat gccaccaacc gagtacaaaa acatattgaa
    33241 caaattgtta tcagaaacac ctatgaagcg caacacattg tcattgaaaa tggaagcatg
    33301 gtctttagtg ctacttgtgt aagagcgaat ctgatgtgga tttttcaatg tgacaaaaag
    33361 gctaagactg tcagtcttga cttgtttatc ttctttggtt atcaaggtat atcctggtgc
    33421 agaagattct tcaaatacgg gctttctaca gatacccaca actgtataag ttttcttatc
    33481 ctgtggcaca aaaacttcac tcccagcagt gtaggggtca ctctgaccaa gcttttcatt
    33541 ttcactcatt cgtttcccta catccaaaga aatagtatca cctactgcaa atttgacacc
    33601 accatctgtc gcaactttac ttgaaacaag aatctctcta ctattttcag gtaatcttcc
    33661 agaaattaaa gttataggca aagtatcaaa agcttctttg ctaaatccag caataaaaag
    33721 atatggttta tctggatttc tgttaccttc aagtgttgca tagccaatat tttcaaatgt
    33781 tgcagtattt gcaacttcat cattgactct ttgttcctgt acaaaagaag aatccacatc
    33841 caaaaactgg acatgccaat caccatattt tataatagct ccatttgtca tatagttcat
    33901 caaagaaact ccaaaggtag taactgctgt aatcatagca gctgacagaa caactcctat
    33961 aattgttaca agagttcgag tgcgattctt tttcaatcct tgcagagcaa ctttgttaaa
    34021 aatgttcatt aacgattcac cttcctctca tcacgcacca cttttccatc tgaaatacag
    34081 ataattctat ctgcctgtaa agcaatgttt tcatcatgtg tgacaataat gagagtttgg
    34141 tgatatttac tatggctctc tttcagtaac tggataatct cttgtccatt tttactgtct
    34201 aagctacctg taggctcatc agcaagcata actgctggtg cattcatcaa tgcacgacca
    34261 atggcaacac gttgctgttg gcccccagaa agttgatttg gtaaatgtgt ttttcgttct
    34321 tttaaaccaa gtaattctaa caagtcattt aaccgttctt gattgacctt acgcttatcc
    34381 ataagtatag gcaatgtgat attttccact acatttaatg tgggtatcag attgtgaaac
    34441 tgataaatca atccaacttg tcgcctacga aaaatagcaa gtttttcatt gctttggcca
    34501 tatacatctt gcccctccag atatatcttt ccacttgttg gtacgtctac tcctgcaatg
    34561 gaatgtagta gtgtagattt accagaacca gaagaaccaa tgattgcagt aaactcccct
    34621 ttttcaatag taagtgagac atggtcaagt gcagtaacct tattttcatt cttaccataa
    34681 accttacata aatcttcaat ctttaaaaac gccattatat aagtctcctt tcatatttta
    34741 taaatatatc atagaacttt caacttacat ttaagtgaca tttaaatgag agattcgtca
    34801 ctttggaaaa cgaataacaa atattgcacc accttgtggg tgattttgtg cagtaattgt
    34861 tcctccttgt ctcataataa tcgtcttaca aagtgccaat cctattccat aaccagctgt
    34921 gcttgaattt ttcccacgat aaaatctgtt aaatagacat ggtaagtctt cttttttaaa
    34981 gcctgctccg ttgtcatgaa tagtaagttg agtaaacaaa aagttgtcct cacaaacgat
    35041 atcaattttt ccattatcac ccacactctc catgcagttt ttaaatatat tttgaattgc
    35101 ttctgaaagc caatttaaat cacccaaaat ccttactcct tttggtatat caatatgtaa
    35161 agtgatattg tgcagttcca tgggaatcaa aagttgatga agtgcagaac taatcaaatt
    35221 attcacatca atttgttctt tttgaaagac tacaatacct gcatctaaac gtgataattt
    35281 taaaagggaa gtaatcagcc agtccattcg tataagtaat tcctctgttt ctcgtatcaa
    35341 ctcttttctc tcatcttcat cagaagtatt ctctaacagt gacaaaataa gagttactga
    35401 tgtaagagga gtgcgaagct ggtgggctat gtcagctagt gagtcagcaa ggtgttcttt
    35461 ttcttttttc agtgcgtaat tttgctctct aatacgtaac gtcattttag ttatttcact
    35521 ttgtagaata gaaagctcac cctcatctga ttcagcaata tacagatggt tagcattatg
    35581 aagcacaaga ttaatttgtt ctgaaatctg tgcaatactt ttgtatcgat ttttagtaaa
    35641 cacaaaaaat gctattccaa aggtagtggc agaaacaata gaaagtattc cagtcataat
    35701 actgattgca aatcccaggg ctacagtgag aatagtaatt aataagaata aaatagcaaa
    35761 ttttcgaaac tctctatttc gaaacataac ttccccccaa tctataccct gtcccacgaa
    35821 cagtcaaaat tatttgtgga cttgaagggt catcttctat cttctctcgc aagcgtttga
    35881 tgtacacagt caatgtgttg tcattaacaa actcacctgc cgcatcccac aattcatcaa
    35941 gtaatcttac cctcgtaata atacttttag gattgctaat aaacaccaat aacaaacggt
    36001 attctaaagc tgaaagaaaa acctcattgc cattcttttt cacaacaccg gttgccgtat
    36061 ctacataaag cccacaaatt tcaaaagttg acccaaaacg cccacttttt cgtagtgctg
    36121 ttctaattcg cgcaatcagt tcacgtggac gaaaaggttt agtaatatag tcatctgcac
    36181 ccatattcag tccagtaaca acacttgatt catcaccaga agccgtcaaa aagataacag
    36241 gaatgtcttg cttttctttg atttctgtgc aaatcgtaaa cccatttcca tcaggtaaag
    36301 aaatatcaat aagtgccaaa tcaaatttat tcttagcaat ctctacaagt gcttcacttc
    36361 ttgtggatac atgggttact ataaatcctt ctgaattaag caaaagaata agatttttgg
    36421 caattgcttt atcatcctca actaaaaata tccgtttcat ttactttcat cctcctttat
    36481 tttattagtt ttatatattc cagcagagca atagtacact actattttac ttatcattac
    36541 acaattttca gtagtttctt ttgttcttat ataatccatg cataatacct ccattgattt
    36601 attatatttc ttataacata aatacacaag ataagacaaa aaacataatg taagtgcaag
    36661 ttttttaact cactaactta taaacataca ctcctactaa cactactttc ttgatgacac
    36721 atcttacaag cttatattca tacatcaatg aaaaattaaa tttatccatt tattaaaata
    36781 aaaggcaata gcttctatag ctaatgccct ttatcttaaa atgatttaaa tactatacaa
    36841 tttttataaa tggctataaa taaactcgta tagaatataa aatttatcta cagtcttttg
    36901 ttatacaaaa tttataacct cttatagaac aaatttacca ctttgtaaac ttatcacttt
    36961 tactcagcta tattataaac ttttaattta tctttgttta aatgtttctt cttaacttct
    37021 actaaagctt ttagcgtatc caaagatgtc attatttcag tagaaaattc aatggctgtt
    37081 cttctcatct taaatccatc tcttgtagag tcatttcctt tagttggtgt atttattact
    37141 atatcaactt gtttatttct tatagcatct aatatgtttg gtctaggctc ttcaattc
//
LOCUS       DAEPOG010000018        35623 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEPOG010000018 DAEPOG010000000
VERSION     DAEPOG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.018"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            94..1368
                     /locus_tag="KR432_000795"
     CDS             94..1368
                     /locus_tag="KR432_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBH0639349.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            1486..2205
                     /locus_tag="KR432_000796"
     CDS             1486..2205
                     /locus_tag="KR432_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0639350.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            2226..2672
                     /locus_tag="KR432_000797"
     CDS             2226..2672
                     /locus_tag="KR432_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639351.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     misc_binding    2820..3066
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3190..4932
                     /gene="leuA"
                     /locus_tag="KR432_000798"
     CDS             3190..4932
                     /gene="leuA"
                     /locus_tag="KR432_000798"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH0639352.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     gene            4889..6166
                     /gene="leuC"
                     /locus_tag="KR432_000799"
     CDS             4889..6166
                     /gene="leuC"
                     /locus_tag="KR432_000799"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH0639353.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            6181..6672
                     /gene="leuD"
                     /locus_tag="KR432_000800"
     CDS             6181..6672
                     /gene="leuD"
                     /locus_tag="KR432_000800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH0639354.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            6679..7749
                     /gene="leuB"
                     /locus_tag="KR432_000801"
     CDS             6679..7749
                     /gene="leuB"
                     /locus_tag="KR432_000801"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH0639355.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            8112..8627
                     /locus_tag="KR432_000802"
     CDS             8112..8627
                     /locus_tag="KR432_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBH0639356.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            9000..10076
                     /locus_tag="KR432_000803"
     CDS             9000..10076
                     /locus_tag="KR432_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0639357.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            10316..11257
                     /locus_tag="KR432_000804"
     CDS             10316..11257
                     /locus_tag="KR432_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBH0639358.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            11289..12539
                     /locus_tag="KR432_000805"
     CDS             11289..12539
                     /locus_tag="KR432_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBH0639359.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            13357..16905
                     /locus_tag="KR432_000806"
     CDS             13357..16905
                     /locus_tag="KR432_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0639360.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            17090..19024
                     /locus_tag="KR432_000807"
     CDS             17090..19024
                     /locus_tag="KR432_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH0639361.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            19130..20125
                     /locus_tag="KR432_000808"
     CDS             19130..20125
                     /locus_tag="KR432_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0639362.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            20097..20882
                     /locus_tag="KR432_000809"
     CDS             20097..20882
                     /locus_tag="KR432_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0639363.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            20870..21568
                     /locus_tag="KR432_000810"
     CDS             20870..21568
                     /locus_tag="KR432_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639364.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            21691..22164
                     /gene="ybaK"
                     /locus_tag="KR432_000811"
     CDS             21691..22164
                     /gene="ybaK"
                     /locus_tag="KR432_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBH0639365.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            22361..23200
                     /locus_tag="KR432_000812"
     CDS             22361..23200
                     /locus_tag="KR432_000812"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBH0639366.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            23213..23761
                     /locus_tag="KR432_000813"
     CDS             23213..23761
                     /locus_tag="KR432_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBH0639367.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            23827..25008
                     /locus_tag="KR432_000814"
     CDS             23827..25008
                     /locus_tag="KR432_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBH0639368.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            25181..25444
                     /locus_tag="KR432_000815"
     CDS             25181..25444
                     /locus_tag="KR432_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0639369.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            25970..26653
                     /locus_tag="KR432_000816"
     CDS             25970..26653
                     /locus_tag="KR432_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639370.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            26664..27905
                     /locus_tag="KR432_000817"
     CDS             26664..27905
                     /locus_tag="KR432_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639371.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            27977..28660
                     /locus_tag="KR432_000818"
     CDS             27977..28660
                     /locus_tag="KR432_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639372.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            28657..30987
                     /locus_tag="KR432_000819"
     CDS             28657..30987
                     /locus_tag="KR432_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0639373.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(31168..32010)
                     /locus_tag="KR432_000820"
     CDS             complement(31168..32010)
                     /locus_tag="KR432_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0639374.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            32305..33039
                     /locus_tag="KR432_000821"
     CDS             32305..33039
                     /locus_tag="KR432_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639375.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            33049..34182
                     /gene="nagA"
                     /locus_tag="KR432_000822"
     CDS             33049..34182
                     /gene="nagA"
                     /locus_tag="KR432_000822"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0639376.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            34281..35030
                     /gene="nagB"
                     /locus_tag="KR432_000823"
     CDS             34281..35030
                     /gene="nagB"
                     /locus_tag="KR432_000823"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH0639377.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            35205..35477
                     /locus_tag="KR432_000824"
     CDS             35205..35477
                     /locus_tag="KR432_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639378.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT        14272 a         3690 c         6437 g        11224 t
ORIGIN      
        1 aatttatttt aaaaattata gtaagaaaaa tgtaagtatt tgcaatatta atggtataat
       61 atttacagat atgataatca ataagaggaa aggatgttta taatggaaaa tgatttaaca
      121 atgcaagaat tacttgacca gcaagaacaa gtattcagca aagtgaaagt tggagaattg
      181 acaactggaa aaataactgc agtgagaaat gatgaagtac aattaggatt agactatggg
      241 tttgatggaa taatacctat aagtgaattg aacatagaaa aaaatcaata tatagaagat
      301 atataccaca taggtgatga aataactgct gtaataacaa aagtatctca aaaagatggt
      361 acaataactt tatctaaatt acaacttgac aaaagaaatg attttgccga gttacaaaag
      421 gcttatgatg aacatagaat aataactgta aatgttgaaa agaatataga taaaggtgta
      481 tttgctaatt acaatacata tacatttttt ataccaattt ctcaacttga tacaaaattt
      541 attacagata catctaaatt tgttggtctt aacttagaag tttatataaa agaattggat
      601 gttagaaaaa atagacttgt agcatctcat agagatgtat tacaagagcg tataaataaa
      661 gagagagaag aaagaagagc tcaaattaag gcagaaaaag aagctgaaag agcaagaatt
      721 aagcaagaaa gagaagaaga aaaagctcgt atcaaagcag ctaaagaaga cttatttaat
      781 tctcttgaag ttggacaaaa aagagatggt aaggtgacaa agataatgcc ttatggtgca
      841 tttgtggata ttggtgggat tgaaggatta gcacatataa ataatttagc ttggacaaga
      901 gttgaaagtg ttgaagatgt tgtttcagag ggacaagaag tagaagttta tgtgttggat
      961 gtaaataaag agactaaaaa aattgccttg gcattaaaag acataaataa tgacccttgg
     1021 gatttaatag caaaagaagt acaaattgat gatgttgtca atgctaaagt tcttagaata
     1081 atagaaaaag gggcttttgt tcaaattaaa gaaggtgtag atgcgtattt accaatatct
     1141 gaattgagtg atactagagt ggctaaagtt actaatgttg ttaatattgg agatgaggtt
     1201 aaagttaaga ttcttgattt taaaccaaag actaaaagaa tgttggtttc aataaaagaa
     1261 gcaactagag agccagaaga ggatataaca gaatacctag aagtagagga atctttagga
     1321 agtattggag aattatttaa agataaattt aaagatttag aagtgtaaaa taattgaaaa
     1381 tggggctgtt ttaaaacgtt acactgtcta ttatgacagc tttagttttg aaatagccct
     1441 ttagctattt tcacgaattt atgtaaaata ttaggaggaa atttaatgaa aaagtctatt
     1501 atggatgtac attgtcacac tctaataagt ggacatgcac atagtacttt caaggaaaat
     1561 gtcgaagagg cgagtaagaa gaatataaaa tacttaggaa tatcagacca cggacctaat
     1621 atgccaggag gaccacatcc attctatttt tataatttgc atttgcttcc acgtgaagtg
     1681 caaggtgtaa agattcttag gggaatagaa ggaaatataa tggattatag tggtaattta
     1741 gatgttcaag aagatatgtt gcaacatttg gattatataa tcgcaagttt gcatagacct
     1801 tgtatagctt ctggtacaaa agaagaaaat acaaatgcta ttttaaaagt tatggataaa
     1861 cctaaggtaa aaataattgg acatccagat gacagtaggt atcctttaga ttatgaacct
     1921 atagtaaaaa aagctaaaga taaaaatatc cttttagaaa taaataattc atcattaagt
     1981 tcaaactctc atagaacggg tacttgggaa aatgtaagtc atatgttgac attgtgtaaa
     2041 acatatggta caagagtaat tcttggtact gactctcata tatgttattc tataggagaa
     2101 tttgaaaatg cagaaaaagt tttagaagca gtagattttc cagatgaatt agtcataaat
     2161 tatcatgaag atgaaattat agaatttttt gatataaatt tttagaatct tgggagtgaa
     2221 aaaatttgag taaaaataag tttatctata tgaattatta tgacaatggt tattttaaaa
     2281 aaggaatgac tatatatatg gttttagcac ttataatttt agctacaatt tttatatatt
     2341 caacgacaat attatatatt ttatgtataa taacagcagt ttttctattt aaacaaggtc
     2401 gtgatttttc aaataaatat gagggaaaag tatatattac tctcgataat cattcaatat
     2461 taataaataa tcaatgtata ttcagtataa aatctaaaca aagtcaaaag tttaattata
     2521 agataataga aaaaatagaa gtagtaaaaa atatattaaa tatatacact gacgaatcta
     2581 attataaaat tagattaagg gcattgagtt tggaagatga aaaaaaatta ttgaatatca
     2641 tagatgaaaa gatgaaaaag tttaaggcat aatatcatac tgttttcaat agttagcatg
     2701 ttttatagaa ttgcttatca aaaataaaaa attgagataa ttattttttt tgttgacaat
     2761 acattttaat gtgtatataa taactattaa atcaaaatat ttagaaaatt taaatgatta
     2821 aaaaagtgat gaatagaagt agtaagaaca atgaaacttt cagagagcta aaggatggtg
     2881 tgatttagta gttgatagtt ctgaacttgt ctaggagctg ttttctaaga tttaaatctt
     2941 agaataatcg gagtaattcc acgttatagg aaatatagtc attataatta ttgtattggc
     3001 tattactaag ggagcttttt agaaagctaa atagagtggt accgcgagca aaacctcgtc
     3061 tctaatttta ttagagatga ggtttttttt attctaatga taaaattgac atttatcctt
     3121 atgaagtgtg caggattgcg tcgatataag gtatactgaa tacaaaaaac agaaaaaggg
     3181 ggaaataaca tgaagtgtgg aaagtataaa aaatatgata aaatgcaaat agtaaatcgt
     3241 aaatggccag ataatgaaat atttaaggct ccaatttggt gtagtgtcga tttaagagac
     3301 ggaaatcagt cactgccaac gcctatgagt gtaaatgaaa aagtcagaat gttcaaaatg
     3361 cttattgata caggatttaa agaaatagag gtaggatttc catcagcatc aaatacagag
     3421 tatacatttt taagaaaatt aatagatgag aatatgattc cagatgatgt aactatacaa
     3481 gtacttacac aatctagggc tcatcttata gaaaaaacat ttgaatcaat aaagggatgt
     3541 aaaaaagcaa ttatacattt atataactct acatctgttc ttcaaagaga tgtggttttt
     3601 aatatgagta aacaagaaat aatagatata gcagttgaag gagcaaaatt atttaatgaa
     3661 gaagtaaaaa aatatccaga aacagaattt acttttgaat attcaccaga gagttttaca
     3721 ggaacagaga tggattatgc acttgaaata tgtgaggcag tgatagatgt ttggaaacca
     3781 actcctcaaa aaaaagtaat aataaatctt ccatcaacag tggaaatggc aacaccaaac
     3841 gtatatgcag accaaataga atggttttgt aaaaatataa gttgtaggga ttctattata
     3901 ctaagtttgc atactcacaa tgatagagga acttgtacgg ctgcaagtga acttggatta
     3961 ttggctggtg ccgatagact tgaaggaaca ctatttggaa atggtgaaag aactggaaat
     4021 atggatatag taaatgtggg acttaatctt tatacacaag gaatagaccc agagctagat
     4081 tttagcaata tagataagat aattggtata tatgaagact gtacaaaact tatggtacat
     4141 gatagacatc catatgcagg taatcttgta cattgtgctt tttcaggttc acatcaagat
     4201 gctattagaa aaggcatgat agcaatgaaa aacagagata atgattactg ggaagttcca
     4261 tatttaccaa tagaccctca tgatattgga agagaataca aagaaattat aaggataaat
     4321 agccaatctg gaaaaggagg agctgtttat ataatggaaa cagattatgg atttatgata
     4381 cctaagaata tgcattctga ttttggaaat gtagtcaaaa tggaatctga tagaattggg
     4441 gaagaactta gtagtgaagc aatatttaat ttatttaaga aagaatatat tgaagttgag
     4501 agcccttata aagttaagaa gtataaaata aaatcaatgg atgaattaaa ctatgaaaat
     4561 gatgattcta atgatactaa catgattgag atgactgcaa gaataagtta tatgggaaat
     4621 gaacaaagga ttgttggtat aggaaatgga cctgtagatt catttaataa tgcactaaag
     4681 caatgtggca tgaaagatta taaatttaga tattactggg aacatgcatt agaggaaggg
     4741 tcacattcaa gaggggtagc ttatgtagga atagagcaca ataatgaagt ctattttggt
     4801 gtaagtatat cggaaaacat aaatacagca gctattaatg cacttatgaa tgcaataaat
     4861 aaaagttata ttgaggagga aataaaaaat ggggatgact atgacgcaga aaatattagc
     4921 caaacatgct aaattaaatg aagttaagaa agggcaatta atcgaagcag atttagatgt
     4981 agttttaggt aatgatataa catcaccagt agccattaga gaatttgaaa aattaggaat
     5041 tgaagatgtt tatgataaga ctaaagtagt gatggtttta gaccacttta cacctaataa
     5101 agatataaag agtgcagaac aatgtaaatt tacaagaagt ttcgcaaaat caaaaggagt
     5161 tgtaaatttt tttgatgtag gagatatggg aatagagcat gttcttcttc ctgaaaaagg
     5221 tatagtaact gctggagatg taattatagg tgctgactct catacttgca catatggagc
     5281 attaggagca ttttctactg gtgtaggttc aactgatatg ggagcaggta tggctacagg
     5341 aaaatgttgg tttaaggttc ctggagcaat aaaatttgtt ttgaagaata aaccaaataa
     5401 atggataagt ggtaaagata taatacttca tataataggg gagataggtg ttgatggagc
     5461 actttacaag tcgatggaat tttgtggaga tggagtagaa tacttatcta tggatgatag
     5521 atttactatt tgtaatatgg caatagaagc aggagcaaaa aatggaatct ttccagtaga
     5581 cgataagact atggaatata taaattcaca taagtgttca actataacaa aagatgtaaa
     5641 tatatatgaa gcagatgaag atgcagtcta tgatgaagta tacgaaatag atttagccga
     5701 actaaaagaa acagtagcct ttcctcatct tcctgaaaat actagaacag tagatgagat
     5761 agataaggat ataaagatag accaagttgt aataggttct tgtacaaatg gaagaatatc
     5821 agaccttgaa gtagtagctg agataatgaa aggcaaaaaa gtagcagatg gagttagagt
     5881 tatgatattt cctggaactc aaaaagtata tcttgaagct atagaaaaag ggtatattac
     5941 cacttttata gaggcaggag cagcagtttc tacaccaact tgtggtccat gtctaggggg
     6001 acatatgggt atacttgcag caggagaaaa atccatttca acaacaaata ggaatttcgt
     6061 aggaagaatg gggcatgttg attcagaggt gtatcttgca agcccagctg tagcagcagc
     6121 atcagctata actggaaaaa tatcaaaacc atcagaaata atttaaggga ggtaaaattt
     6181 atgattgcca atggaagtgt ttttaaattc ggtgacaata tagatacaga tgtaataata
     6241 ccagctagat atttaaatat agctgattat aaagaactag caacgcattg tatggaggat
     6301 attgatgata aatttatttc taaggtaaaa aaaggagata ttatagttgc aactaaaaac
     6361 tttggttgtg gttcatcaag agaacatgca ccaattgtaa taaaagaaag tggagtatcc
     6421 tgtgtgattg cttctacttt tgctagaata ttttttagaa actctataaa tatagggctt
     6481 cctatacttg aatgtgaaga agctgccaat aacatagatg aaggggataa tatagaagta
     6541 gatttttcta caggtgtgat aaaaaatata acaaaagaaa aagaatataa agcagaacca
     6601 tttcctgagt ttatgcaaaa tattatatta aatgaaggct taattaactc aataaaagca
     6661 aatagggggt agtggagtat gaattgtaat atagcagtca taaaaggtga tggtgttgga
     6721 ccggaaatta tagatgaagg tataaaggtt ctaaataaaa tttgttgtaa atttaatcat
     6781 agattcgatt gtgaatatgt attagctgga ggatgcgcga ttgatgaaat tggagaacct
     6841 ctacctaata aaactgttga aacttgtcga aaaaacgaag ctgtattgct tggtgctgtt
     6901 ggaggaccaa agtgggataa atgtaaggga gataaaagac cagagtctgg tcttttaaaa
     6961 ttaagagagt cattgggttt atttgcaaat ttaagaccag ctacaatgta tgaaagtata
     7021 aaagaagctt caccacttag aacggatata gttgagaaag gcattgattt tgttgttgta
     7081 agagaattaa cagggggcat ttattttgga gaacgtggaa gaaaaatcat tgatggtatc
     7141 gaaaatgctt atgatgtaga aatatataac gaaaatgaaa taagaagaat tggtaagaga
     7201 gcttttgaaa tagccagaaa tagaaataaa aaacttataa gtgtagataa ggcaaatgtg
     7261 cttgagagtt caagactttg gagaagtata atggaagact tagctaaaga gtttgaggat
     7321 gtagaactgt cacatatgta tgttgataat gctgctatgc aagttgtaaa agaccctagc
     7381 caatttgatg taatagtgac taataatata tttggagata taatatctga tgaagcatct
     7441 atgattacgg gttctatagg tatgttacca tcagcatcac ttagagaaga tagttttggt
     7501 atgtatgaac caatacatgg aagtgcgcct gatatagctg gaaaagatat tgtaaatcca
     7561 attgcaacta ttctatctgt aagcatgatg ttaagacata gttttaactt agaagaagaa
     7621 gctaagtgta ttgaagatgc tgtacaaagt gtactcaata aaggttatag aactattgat
     7681 atctataatg gtgtgggaaa tgttgtagga acaagagcaa tgggcgaatt aatagtaaat
     7741 gaaatttaga attatagttt aatttgaata atataattta aatatataac ttgtagaatt
     7801 agtaaaaatc tattgtagta taatttgttt tatgaggggt gtctcaaaat aaactttatt
     7861 agttaatgag acactctttt ttatattaag tcaaatatat ttttactatt ttaatagcga
     7921 aaaagaggct tatatctact ttgagtttct attttgataa agttattttt tggaaaataa
     7981 tactttaaat taaacttatc atattgaagt agcctatttt tattattaga agtttgcagg
     8041 gattttatta atattatata atatatttta gaattattta atattaagcg actactaatg
     8101 tcggttagga gatggatttt ttggcaaagt ataaagtttt gaagaagcaa aatagtagta
     8161 aatcttgttt agtttgtgga acacaaaatg agctaggact taaagcagat ttttatgaac
     8221 ttgaaaatgg agagcttgta tcaatttgta atacaaagga ttggcatcaa agttatcctg
     8281 ggagagttca tggaggtatg tctgctgcaa tattagatga gactattgga agagctgtaa
     8341 gtataaatga tgaccaaata tggggagtaa cagtatcttt agagttgaaa tataagaaac
     8401 cagtaccaac agatgctact ataaaagttg ttggaagaat aactaaagaa aacagaaaat
     8461 tatttgaagg tacaggagaa ataattctac caaatggaga tatagcagtg acagcaactg
     8521 gaaagtatat gaaaatgcct attggacaaa ttgcagaagg tgatttttct aatgaagaat
     8581 ggttttttga agaatcgaaa gaaaaagtgg aatatattga actttgatta aataaaaatt
     8641 ttatatttta aaacataaat tttatattta gaaaaaataa aattcaaaaa tacaataaaa
     8701 atacaagata aaaataaatt agttatacta aaaagattta tcgtaatagc gtcaaattta
     8761 catactaaaa aaattaattt atatagataa aaagtatatt ttttttgaga gaataattca
     8821 gaaaattcag acttatgaaa aggcagaaaa tgaatgtttt cataaaatat ataaaataaa
     8881 ttatatataa aaaataaatt ttatgttgac atagttaatt cagcttgata ttatagttat
     8941 taaaaataag gacagtaaaa acgctatcca tattacaaac taaaaagggg ggaattataa
     9001 tgagtaagaa attatttatt cctggtccta ttgatgttaa agaagaagtc ttgcaaaaga
     9061 tggctacacc aatgatagga catagaggaa aagatgcatc tatgctccaa aagagtataa
     9121 gtgaaaagat gcaaaaacta ttctatacaa acaacacgat acttttatcc acgtcatccg
     9181 gaactggttt aatggaaggc tccattcgtt cctgtacatc caaaaaggca gcagtatttt
     9241 cctgtggatc ctttggtgat agatggtaca aaatggcagt ggctaataat gtcccagcgg
     9301 atatatttaa agttgaatta ggagaggcaa caacgccaga aatggttgat aaggtgcttt
     9361 ctaccggaga atacgattta ataactgtaa ctcacaatga aacttcaact ggaataagaa
     9421 atccaataga agaaattgga gaagttgtta aaaagtatga agatgtaatc tactgtgtag
     9481 atactgtaag ttcagccggt ggaataaaag tcgaggttga taagattgga atagatattt
     9541 gtataacatc agtacaaaaa gcactgggat taccaccagg aatgtctatt tgcacatttt
     9601 ctcaaaaagc aatcgataga gctaagcaag taccttttag aggagtatat cttgatttgt
     9661 tagcaatgta tgaatactta atcaagaaaa actaccaata cccttcaaca ccatctcttt
     9721 ctcacatgtt cgctttagat ttccaattag ataatatatt agatgaaggc ctagataaca
     9781 gattcaacag acatgaagac atggcaaact tagttagaaa ctgggctaaa aaacacttcc
     9841 aaatttttac caatgaaaat cacttatcca atacacttac tgttatagaa aatactcaag
     9901 gtattagtgt atcaaattta aattcaaaat tacaagaacg aggattccaa atagcaaatg
     9961 gctatggaga tttaaaagaa aaaactttta gaatttcaca tatgggtgac tatactgtcg
    10021 aagatgtaca agaattatta gataatattg atgatatatt aggttttaat aaataatatt
    10081 gtaaaaatat gtatgtttta agataaattt agaatatata ataaataata ttttataaaa
    10141 attacatatt aaatttttaa caaacaatag gaaaatttga tatgacttta ataaaaaaat
    10201 attttgaaaa ttatataaac agaaagttaa attaagtaaa gttttttatt gatgaaaata
    10261 ctttaaaaaa taaaaattgg aaaaattaag tgaataaaaa ttggaggagt ttgttatgta
    10321 taatatatta gttactgatg gaatagagaa agaagcagcg agaaaattga gagagttaga
    10381 ttttaatgtg atagagcagt tttatgaaaa agatgtttta ggagataaac ttaaagatgt
    10441 tgatgtactt gtagtacgtt cagctactaa agttactaaa gatgtaatag ataaagcatt
    10501 agagggtaaa aagttgaagt tgatagtaag aggtggagta ggtctggata atatagatgt
    10561 taaatatgct caagctaatg gcataaaagt tatgaatact ccaaatgcaa gcagtatatc
    10621 tgttgcagag cttacaatag gtcaattatt tgttcttgca agattcataa atactgcaaa
    10681 tgtaactatg agagatggaa aatgggagaa gaagaagtat aaaggtacag aaattaatgg
    10741 aaagacactt ggacttatag gttttggaag aattgcaaaa gaagttgcaa agagagcaga
    10801 attattaggt atgaatgtta tatatactga cataatgggt gaggcgcaag gttttaacaa
    10861 ttataaattc tgtgatatgg aagaagtttt agaaaatgca gatttcttat ctttacacat
    10921 accatttgat aagaataagg gggcagtaat aactgaaaaa gaaataaata agatgaagaa
    10981 aggggcttat ttaataaact gtgcaagagg cggtttagtt gatgaaaaag atttactaaa
    11041 agcattagat gaaggtaaat tatcagcagc tgctatagat gtttatgaac aagaacctac
    11101 attaaattta gacttagtaa atcatccaag agtttctcct acacctcata ttggggcatc
    11161 tacagtagaa gctcaagaaa gaataggaga cgaaatagta aatgtgattc aagatttctt
    11221 tcttgacttc aacaatctaa taggggtagc attataaaat attttgtata attaggagga
    11281 aattagtaat ggcaacgatt aaaccattta aggcgattag accaaataaa tatatagtag
    11341 ataaagtagc agcacttcct tacgatgtta tgaatagtaa agaagctaga agaattgcag
    11401 aagggaatcc atattcattt ttgcatatag ataaatcaga aattgattta gatgaaaata
    11461 tagatttata tgatgaaaaa gtttatttaa aagcgcgaga aaatttatat gagtttcgaa
    11521 aacaagaaat tctagtaaaa gatgacaaag agtgtatata tatatataaa caaatcatgg
    11581 atggtagagc tcaagttggt atagtagcat gtatatcagt tgatgaaagc ttgaatggaa
    11641 caataaaaaa acatgagtac actcgaccag aaaaggaaat tgatagaact aaacacataa
    11701 aatactgtga tgctaatacg ggaactatac ttgtaactta caaacatcaa agggtgatag
    11761 atgatataat caatgatttt atggataata atgaaccttt atatgatttc ataacagatg
    11821 acaagataga gcatacagta tggaagattg atagtgatga tataatagat aacctagtag
    11881 ataaatttga aaaattagat tatctataca ttgcagatgg tcatcatagg acagcttcag
    11941 cagaaaatgt agccaaagaa atgagagcta aaaatccaaa ttatactggc aaagaagaat
    12001 ttaattactt catagcaatg attgcaccag atgagaattt aatggttttg gattataata
    12061 gggttatcaa agacttaaat ggtttaagcg aggaagaatt tataaataaa ataaaagaaa
    12121 attttgaact agaagaaata gaaggaaaag aaaaatataa acctgacaaa aaaggaactt
    12181 tcggaatgta cctaggtgat aagtggtata aaatgaaagc aaataaaaat ttattagaaa
    12241 tagaagaccc tgttgactca ttagacattt ctatattaca agactatgtt attgatgcta
    12301 ttttaggaat tgacaatcct agagtagata aaagaattga ttttatcggt ggaattagag
    12361 gtcttgaaga attagaaaaa agagttaatg aagatatgaa agtagcattt gcaatgtatc
    12421 ctactagtat tgaagattta ataagagtag cagatgcaaa taagataatg ccagctaagt
    12481 caacatggtt tgaacctaaa gtaagatgtg ggctattctt acatgaaata aatgaataat
    12541 taataaaggg ctaaaaagtg tacaattcac tttttagccc ttgttttttt gattgaaaat
    12601 cgtatgtgga atataaattc tataagttga tattcttaat aatagttacc tttttttaga
    12661 aatggataat tttaggatat agatagtcat attctatgct ataattgagg atgaaaattc
    12721 atataattgt atttgtatat ttataaggtt ttaaatatgt atttataata gtattgttat
    12781 atattttatt ataattataa aaaagttaat aaaaaaagtg attttgaaat ttatatttta
    12841 tttttataaa tataattatt atatttataa aaataaaata gtaaaaggat attataatat
    12901 tttactagaa ttattatctg gtaacttaag ttttgagtat taatctaaaa tatatgtttt
    12961 tatagggttt aaattttatg tatacaaaat taatttgcta actgaatcat ataaaaaaaa
    13021 caaaatattt tttagaggga atgattagat actattttct ttgttatgaa attaattgaa
    13081 gagtaattaa ttagaaaagg aataaataat aatgaatata ttacattatt actttttgat
    13141 aaatcaaaaa taaagttata gtttttaata ttaattaaga atgatgaaat atgaataaag
    13201 attttacaaa taaatcttaa gctatcttat ttaattctaa tgcaggaaga aggtaaatgt
    13261 atattgaaaa aatcatagaa atagaagtaa acaacttaaa caaatacaaa ggtttgcaaa
    13321 agaagtaata taattatata caatgaggat gtgagtatga tgaacagact aaagccaaga
    13381 attttaggta ttagtatctt gtttattatt ataattattt taacatgtgg agctgttttc
    13441 aagtttaaat ttaaattaga taataaatta aaagaaacca cacatacata tttgtctgag
    13501 tttacagaac acaatataat taatctcaag acaagactta gtggtcaatt tgacatgctt
    13561 gaatctattg ctaacttcat aggaaacttg aataatataa ataatgaaat ggtgattgct
    13621 ttaatggatt ctgatgtaaa gcgaagttct ttaattagaa tgactatctc tacattagat
    13681 ggggaatgtt attctaatga tggaatcacc cataatgttc aagatagaga ttattttaaa
    13741 aaagctaaaa atggagaaaa gaatatttca gagcctttaa aatctattgt taataataaa
    13801 gaaataatag ttttatctac cccagtttat aaaagtggga aaatagttgg tgttttaagt
    13861 ggaatttatg attcaaaaaa gttaaatgag ttattaggat tatctgcatt tgatgaaaaa
    13921 gcagatgttt ttgttgcaaa gaaaaatgga gatatagttg ctcaaaaaac aaataaaaat
    13981 agaaagacga tatcatgtat atttcagctt tttgacaaag ataattgtga tgaagatgat
    14041 tgtctaaaaa ctataaaaga aaatatgaaa aatgataagt ctggatattt taattataac
    14101 ttagataatg aaaaatttat gttaaactat cagccacttg gagttaatga ttggtatgtt
    14161 ttttctgttg tgccaaataa aatagtgtct attcagtcag agaatatatc ttatgatgta
    14221 tatatttttg cattagaaat actactagtt atattgattt ttataatata tatagcatgt
    14281 attataaata ataacaccaa aattattata aatgaaaaag aaaaatttaa agtactcact
    14341 gataatatat taggaggggt taaaacctgt ttaaaagatg aatatatgac tatgacatat
    14401 gtaagtcagg ggtttttaga gcttacaggt tactcttgtg aagatttaaa taaaatattt
    14461 aaaaatagat ttaaagacat gatttataaa gatgatttag acaagtttaa ttgtattatt
    14521 ctaaatcaat tagaaaaaca agataaaatt gaagttgaat atagaattgt aagaaaagat
    14581 ggaagtataa tttggatttt ggagagaagt aagtctataa atgataataa aggaagtgag
    14641 tgtattcagg gagttttaac tgatataaca gaacttaaga aaatacaaga agagttagat
    14701 tcaaaaagat ttgaaataca ggctatcaat gatagtattg ttggtggaat agtgataact
    14761 gagataaatg atgattttga tagtatatat ttaaatgaag gttatttaaa tatgattgga
    14821 tatacacgca aacaactgga agatgagcta aataataaat taatcaaact tatttttaaa
    14881 gatgatagaa cagaattaat taatagtata aacaaacaac tcaaattaag tgatacattt
    14941 gcatctgaac aaagagttga aaaaagggat ggtactatta tatggatttt attaaaagga
    15001 agaaagataa tttatgataa tggaaaatct gtactttgtt gtgttattgt agatatcact
    15061 tctgctaaat tattagagca agaacttaaa aagagtgaag aacgtcatga aatcattcta
    15121 aagcagacgc aaaatatcat aatagaatgg gatatagaaa aagataaaat gaatatatca
    15181 cctaattggt ataataagtt taaatatgac cctataactg aaaacgcaac aaaaaatata
    15241 attaaatcag tagatatata tcatgatgat atggataagt ttatttcatt aattaaactt
    15301 acttcacaag gtagtgagta tgaagagtgt gaaattagac ttagaactag agatactatt
    15361 tacgcttggt ttagtataaa ggcaacaact ttatttgata ataatggtaa accttataaa
    15421 gtagtaggaa gtttgactga tattgatgaa tcaaaacgag aagctgaaaa gttgaaagaa
    15481 agggcagaaa aagaccctct tacagatttg tataataaaa aagcatcaca atcacttata
    15541 gaagagtaca taacattgga taaaaaaaga aaaggtgctt taatgattat tgatattgat
    15601 aactttaaag gaataaatga taatttagga catttatatg gggatgctgt attaagtgaa
    15661 attgcaaatg acctaataaa tctatttaga gcaaccgata tagtaggcag aattggtgga
    15721 gatgagttta ttgtatttat aaaagatatt tctgaaacaa gagatattat aaataaagca
    15781 gaagaattgg taaaaacatt tgaaaggtct tttttaggta aaggacgaaa ttataaaata
    15841 tcattaagtg taggaattgc taagtttcca caagatggta tggactatat gacactattt
    15901 agaaatgcgg atatggctct ttactcagcc aaagggaagg gtaagaattg ttatacattg
    15961 tatgataaaa aaattgataa agtccaatat ataactaata caagaaatgc aggagatagt
    16021 gataatgtag agatgtcttt taaagaacat atcacaaatt atatatttga tatactatat
    16081 gaatcaagtg atatagaagt ggcagtaaat ttaattctaa gtattgtagg taaacacttt
    16141 aatgtgagtc gcatgtatat atttgaaaat tcagatgata atcgagtatg taaaaataca
    16201 tttgaatggt gtaacgaagg tgtaacctcg gaaattaata atcttaaaaa tatttgttac
    16261 aaggatttag gcaactatct tttaaatttt aatgcagatg ggttatttta ttgtaatgat
    16321 attgaaaatt taggtagtga tgtgaaaaaa gtacttaagc cacaaggcat acgttcaata
    16381 ttacaatgta aaattttaga gggcaatgaa tttagagggt tcataggatt tgatgaatgt
    16441 tcatataatc gtatgtggac taatgatgaa gtgaacactt tgacatttat atctaaaacg
    16501 ctcagtatgt ttttattaaa aatgcgtatt cagagtaagc tagaagagtc tttaacaatt
    16561 caaaaatctg tgatggataa tatggatata tgggcatatg ttgttgacag gaatacctat
    16621 gaattattat ttatcaataa aaaaatgctt gagtcagcat caaacactca tgtaggtgat
    16681 ttatgttata aagcgatatg gaataatcaa gataaacctt gtaaaaaatg ccctatgata
    16741 tatttaaaag atggtgatga tacttataaa tcatttatgc gcaataaaaa gtttgaggta
    16801 caggcaaata taactacaac tactttgaaa tggtttgatg ggaaagatgc atgtttaata
    16861 tttggaagtg aagtaactag tattgaagct gaagaaaata cttaaaatac tgtgttatat
    16921 atatacttaa aatcgcaatt tgaatttatt agacttaaaa tgtctatata aatcatttaa
    16981 gttgtggttt taagtatttt ttatgatata attaagcgtt aagaacaaaa ttaaaagata
    17041 cttttgtata aaaaaatatt tgttaaatat atattgagca ggtgataata tggaaaataa
    17101 gtttttacca ataagcaaac aagatatgat agatagagga tgggaagaat tagattttgt
    17161 tctagttaca ggagatgctt atgtggacca tcatagtttt ggaacagcaa taatatctag
    17221 agttttagaa aatgctgggt ataaagttgg aataatagct caaccagact ggagaactac
    17281 agatgatttt atgaagctag gaaaaccgag acttgcattt ctagttaatg caggaaatat
    17341 ggattcaatg gtcaatcatt attctgtaag taaaaagcat agagaaaagg atatgtattc
    17401 tccaggtggg aaaatgggtt atagaccaga tagagctact atagtatatt gcaataaaat
    17461 aagagaagca tacagtgatg ttgcaatagt aataggtgga attgaagcga gtttaagaag
    17521 atttgcacac tatgattatt ggagtgataa ggttagaaag tctatgttaa tagatagtgg
    17581 agcagattta ttagtttatg gtatgagtga aaagcagatt gtagaagtag ccaatgcact
    17641 taatgatgga tatgacccaa aatatattag acatattgat gggacatgct atatttcaga
    17701 tacattggaa gaaatatatg ataaatatat attaattcca tcatataaag aaatttgtga
    17761 agataaaatg aagtatgtag aagcttttaa aattcaatat gatgagcaag acccatttag
    17821 aggtaatata attgttcaac ctcatggaag taagtatctg gttcaaaata agccagaaaa
    17881 accacttagt agagaagaac tagatgaagt atatggatta ccatatcaga agacatacca
    17941 tcctgtgtat gaaaaatttg gaggaatacc agctatagaa gaagtaaaat ttagtatagt
    18001 aagttcaaga ggctgttttg gtagttgttc attttgtgca ataacatttc atcaaggtag
    18061 agcagtgcaa agtagaagtg aaaaatcaat tattgatgaa gcaataggaa taacaaattt
    18121 agatgatttt aaagggtata tacatgatgt tggaggtcca acagctaatt ttagaagacc
    18181 agcatgtaaa aaacaaatta ctaaaggtgc ttgtaaaaat agacaatgct tatctccatc
    18241 accatgtaaa aatttagatg cagaccacag tgaatactta catttactta gagctgtaag
    18301 aaaattacct aaaataaaaa aggtgtttgt gcgttctgga attagatacg attatgtaat
    18361 ggcagataag aacaataaat ttcttagaga gttgatagag catcatgtaa gtggtcagtt
    18421 aaaggtagcc ccagaacata tatcagaaga agttttaaag tatatgcaaa aaccagcagg
    18481 aaaaacttat gataaattta gacagaagtt ttttgctata aatgaagaat taggtaaaaa
    18541 gcaatatttg ataccctatt taatgtctag tcaccctggt tctaccttaa atagcgctat
    18601 agagttagca gagtatttaa gagatactca ctaccaacca gagcaggttc aggattttta
    18661 tccaactcca gggactctat ctacaaccat gttttataca ggtattgacc cgcttacaat
    18721 gaagccagtt tatgttccta aatcaaaacg tgacaaggca atgcaaaggg ctttattaca
    18781 atatagagca cctagaaatt atgatttggt atactctgcg ttagttgaag caggaagaga
    18841 agacttaatt ggttttggac atagatgtct tataaagcca aaaaatgaaa agccttattt
    18901 taatagaaat aattctaaaa aaaatgtatc taaaggaaca aataaaaaca aaaaaactaa
    18961 tacaaataac agaagtaata aaaatcaaca aaaaagttca actaaaaaaa agaaaaaaca
    19021 ttaaagtata aataaaaata cttaaaaaag aaggattatt cgataaatta acagaaatta
    19081 gtataaataa atataattat tttaatgacg ttacctttgg gaggaagaga tgaaaaagta
    19141 tatttctata atattattag tggtggtgac tatggttgtt ttggtaggat gttcaccagg
    19201 aaaagataac cctaaaaata aggagctatc agttgtcaaa caagtaaaag tagctgttcc
    19261 agatggacta ccagcagtgg caatagctaa acttgcaaat gaaaatccag agattaaaga
    19321 aggatatgaa acagtgtata gcattgaaaa gacacctgag gcgatttcca ctagagtgat
    19381 gaaaaaagat gcagatatag ctatagttcc ttcaaacatg gctgcaatag cgtataataa
    19441 aacttcatca tataatattg ttggaactgt aggaatgggt tcactttatt tggtgtcaac
    19501 agaaaatata aaagattatt ctgatttagt tggtaaagaa gttggatgta ctggaaaagg
    19561 acttactcca gacataacta taaaaagtct tttgttacaa aaggatataa attattcaaa
    19621 tataaaattt aattatgtaa attcagcaag tgaactggta ccattattgg ctactggtaa
    19681 agttaagaca ggtattgttc cagaacctgc acttagtgct ttgatgtcta aaaatccaga
    19741 cataaaaatt ataaaaagtt taaatgactc atggaaggaa gtaagtggtt caaaagacgg
    19801 ttatcctcaa tctactttaa ttgtaaagtc tgaattttta agagataata aagattttgt
    19861 tgattctttt gtaggtcaat tatcaaatag tatagattgg gcaaacaaaa atcctgaaga
    19921 attaggtgca tattctgaaa aaataaaaat atctactgaa tcaaagataa taggaaaatc
    19981 tcttgaaaga gctaatttaa aatatattcc agttaaggac atgattaaag attataaaaa
    20041 ttactatgag aaattagcta attttgatga taagacatta ggaggcaaag taccagatga
    20101 agcaatatac tttgttgaga aataaaaaaa ataaagaaca atttatagat aaaatggagg
    20161 taacactttc ttgtgttatc ctcttatttt tatggcaaat tattgcttta aaaattgata
    20221 atgatatttt tttaccaaca gtaggtcaag tatttaatag tataaaagag attatgctaa
    20281 attctagttt ctatatagat atactttact ctatggggag atgttttttt agttttttac
    20341 tggctatgat ttttgccatt atactttcaa tagtatctta tttaaataga ttttttagaa
    20401 acttattaaa gccaatcaat gctttgacac gttcaatacc aactatgata ttggtagtac
    20461 ttgcacttat ttggtttgaa aaggatagta ccccttttat agttggattt actatagttt
    20521 ttcctatatt gtatgataac gttttgggcg ctatattgaa tatagacaag aacttattag
    20581 aaatggctaa tgtgtataaa ataaggttta tagataaggt gttaaaaatt tatttaccag
    20641 ccataaaatt tcaaattata tctatactag tatcaacatt ctctttaagt ttgaaagttg
    20701 ctatagcagg agaggtatat agtcaaccta catatggtat tggaactatg atacagactg
    20761 aaaaaattaa ctttaataca tctggaatat ttgcatggat aattattgtt cttttaatat
    20821 ctgctatttt acaaatagcc caaaagtttt tagcaaggag agctttttta tggaaaagat
    20881 aaagttgagt attgaaaata taaataaaag atatgatagt cgtattatat ttagagattt
    20941 taatattgat ttttatgtaa atgaggtaaa ttgtatcctt ggaaaatctg gttgtggaaa
    21001 aactacactc ttgaacataa taagtgggat tattaaaaat gacaccaaca atctaaatat
    21061 caaagaaaat ttaaatagag taggaaataa gcttgaagct agttacatat ttcaagatga
    21121 tagacttata gactggttaa cagtagaaga aaatataaag atagttgtaa ataaatacta
    21181 taataaaact caattaaata aaatttgtga tgaatacttg gaattggttg gaattagtga
    21241 ttataaaaaa ttctatcctc agatgcttag tggaggtatt agacaaagag taaatattgc
    21301 tagagctttt atatatccat caaaaaacat tataatggat gaacctttta aatctattga
    21361 tgcaaagaat acacagctta taatggataa tttcagaaat atattaagaa aagaaaagag
    21421 aactgtatta tttgtaactc ataatattga ggaagcattg tttttagcag ataggatatt
    21481 tattttagga gactctccaa ttagaataaa aaaaatatta aaaaattcta aggaattgga
    21541 aaaaaatgaa gttttgaagt tgatatagag ttataataat actgtcgttt taaaatatat
    21601 ttggttaaaa tctataagac tatattaaaa tagtacagaa tattcaaaac tgtgtataat
    21661 gttcgtattt tataaaaagg aagtgaatgt atggttaaga ctaatgctat gagaatatta
    21721 gattcaaata agattgatta taaagtaatg tcttatgaag tgaaaagtga acatgtggat
    21781 ggtgttgagg tggcacatga tataggaaga gatgtaaatg aagtgtataa gacattagta
    21841 actcaaggag ttagtaagaa tatatatgta tatgtaatac cagtacatga aaacttagac
    21901 ttaaaaaagg ctgcaaaagt agctaaagag aaaagtgtag agatgattca tgtaaaagat
    21961 ataaataagc ttacaggtta tattagaggc ggatgttctc ctataggtat gaaaaagctt
    22021 tataaaactt ttgtaaatga aagtgcaaag aatttagata caattattgt gagtgcagga
    22081 aaaataggat atcaaataga actatctcca tttgatttac aaagacttat aaaagtagaa
    22141 tttgtagatg taataaaaaa ataagaatta catataaaat atagagctta tattcttaat
    22201 aataggtgtt gtaatattaa taaaatgaat aataatttta aaataaatat tcagttactt
    22261 atgtgtaatt ggtataaaaa tattgagaaa ttttaaaaac aatggtaaag tgtaagtata
    22321 atcacgaata ttttttataa agtaatggag ggatgttcgc ttgagaaaaa taaaatctga
    22381 acaaatcgtc gagcaggtaa aaaaactttg tatagaagcg agcctatatt taggagaaga
    22441 tgttttaagt tgtataaaag aaaaagcgaa aagcgaaaag agtgaagttg gaaaaaatat
    22501 attgaatata ttagttgaaa atgctgaaat agctaaagaa aaaaatatac caatatgcca
    22561 agatactggt atggctgtat tttttgttga aataggacaa gaagttttaa ttgaaggaga
    22621 cacacttact gatgctataa atgagggagt aagacaaggg tatgaagaag gctacttaag
    22681 aaaatctgtt gtaagtccaa ttaacagagt aaatacaaaa gataatacac ctgctgttat
    22741 tcattatgat atggtaaaag gtgataaaat taaaatagaa tttgcagcta agggatttgg
    22801 aagcgaaaat atgagtaaga tgaaaatgtt aaagccatca gatggattgg aaggaattaa
    22861 aaaattcata atagatacag tttcagaagc tggacctaac ccatgccctc ctatggtaat
    22921 tggagttggt ataggtggga ctgtggacaa atgtgctcaa atagctaaaa aggcactttt
    22981 tagagaacta ggtgaattta ataaggacga aaatatagca aaacttgaaa gtgaactatt
    23041 aacagctatt aataaacttg gtataggacc tcaaggctta ggtggtacta caacagcttt
    23101 gggattaaac atagagactt ttcctacaca catagcaggt ctacctgttg ttgtaaatat
    23161 aaactgccat gcatcaagac ataagaaggt agtaatttag gaggaaaata aaatgataaa
    23221 aataactaca cctgtaaatg aaattgatat agcaaaatta aattgtggag atacaattag
    23281 tttgagtggt atactttata cagcaagaga tgctgctcat aaaagattga tagattgtat
    23341 aaataaaggt gaagaattac catttgacgt atatggtcaa ggaatatatt atgtaggacc
    23401 tactcctaca aagccaggag aagttatagg tgctgcaggt ccaacgacga gttatagaat
    23461 ggatgatttg actatacctc ttttagaaag aggtcttaga ttgatgatag gcaagggaaa
    23521 aagaagtgat gaagttatag aaggtatgca aaaatacgga gcagtttatc ttgctgcaat
    23581 aggaggagct ggagcgtata tttcaaattc aataaaatca tgtgaaatta ttgcatatga
    23641 agatttagga gctgaagcta taagaaagat tgtagtagaa gaccttaaac ttacagtagc
    23701 aatagatagc tatggaaata atatatatga gcaaggtcga gcaatttatg aatgtaaata
    23761 atgtttagaa ataaatatga atatcaaaat taaaaatagt tgtgtatatt ttaaggagga
    23821 aaattgatgt ctaaggatta tgcaaaattg gcattagaaa tgcatgaaat caacaaaggg
    23881 aaagtaagtg tagaaagcaa ggtagaagtt aaaactaagg atgatttatc aacagcatat
    23941 acaccagggg ttgctgaacc ttgtttaaaa atacatgaaa atcaagatga tgtttatata
    24001 tacacttcta aagggaatct agtagcagta gttagtgatg gaagtgctgt acttggatta
    24061 ggtaacattg gtgctgaagc atctatacct gttatggaag gtaaagctat attatttaaa
    24121 caatttgcag atgtagacgc ttttccaata tgtttaaaga ctaatgatgt agatgagata
    24181 gttaaatctg tagagttgat ggagcctgta tttggaggta taaatttgga agacatatct
    24241 tctccaagat gttttgagat agaagagaga ttaaaaaaat ctttaagtat acctgttttt
    24301 catgatgacc aacatgggac agcaataata gttgcagcag ccataataaa ctcaataaag
    24361 ttaattgaaa ataaaaaaat agaagattta gaaatagtta taaacggagc aggagctgct
    24421 ggtatagcca tagcaaagat actacttaat atgaatgtta aaaatataat cttatgtgat
    24481 agaagtggag cattggaagc tagtatagaa aatctaaact atgttcaaaa agaaatgtta
    24541 aaagtaacaa atataagaaa tgaaaaagga cctttaaaag atattataaa aggaaaagat
    24601 gtatttattg gtgtttcagg tccaggagct gttacaaagg aaatggttga aagtatgtct
    24661 gagaagccaa taatattggc aatggcaaat cctactccag agataatgcc agaagaagca
    24721 aaattaggtg gagcatttat aatgggaaca ggtcgttcag acttccctaa tcaagtcaat
    24781 aatgtactgg cttttcctgg aatatttaga ggagcattag atgttaaggc taaagagata
    24841 aatgaagaaa tgaagatagc tgctgcatat gcaattgcta atacaataag tgatgaagaa
    24901 atatgcccag agtatatatt accagatgta tttaataaga atgtagtaaa aaatgttgct
    24961 aaggcagtta aagaagcagc gataaaaact aaagtaaata gaatataaat ttttataact
    25021 tattaaaaac tagaaaagta taaaagacta gtgaaattat gtacagtggg atgattttat
    25081 tagtcttttt attttaaata tgacacaata atgtcatatt aagcttgata taattttaat
    25141 atggttagga gccaaagaaa aatataagga gtgatttaaa atggaagaaa ctaaattttg
    25201 ccaatgctgt gcaatgccaa tgggacaaac tgatgaatta tatgggacaa ataaagatgg
    25261 aagtaaaagt actgattatt gtagttattg ttttaaagat ggtgagttta cagctgatat
    25321 aagtatggaa gagatgatag aagtttgtat ccctcatatg cttcaaagta ataaggatat
    25381 gactgaagat gaagcaagaa caatgatgaa caatttcttt ccaacattaa aacgctggaa
    25441 ataattttat gcaaaattag ttatatgcaa aacatgaaat atggtgttga gaataagggg
    25501 tggtgtgctt tatagcatac cacctgtttt gttacttgct agttcagtta ttcttgtatg
    25561 cttaaatatg ttctcatctc tctttctatt ttaaattgta aaaatctaaa atatgttatt
    25621 tatattctac ttctttatcc atgtcttttt ctacctcaag tcaaatacaa ctacaaaatc
    25681 atcattttca attcactgtt taatagaatt aacagataaa gagttataaa attttctatt
    25741 ttttatataa aataatacag gtcaagctga cttctagcta cttgggatag tgtatatatt
    25801 tatgatagtt ataggagtaa taccaatcat tagactgttg atttgtgtag tcttttgtaa
    25861 aaacatttgt tttaatatat tgagtttttg aaaaagtaaa attataatta taataaatta
    25921 agtgtaataa tgtattagaa gtaaaaataa ttaaaataga ggtgattaaa tgagcagtat
    25981 attgttagtt gaagatgatt taagcttgat atatggatta gagttctcta tacaaaaaag
    26041 tggatttagt atagacatag caaggacagt aaaagaagct atgcaaatgt atgaggaaaa
    26101 aaattatgac ttattgcttt tagatgtatc cctacctgat ggtaatggat ttcagatttg
    26161 cgaaaaagta cgagaggttt ctaatatacc cattatattt ttgactgctt cagatgaaga
    26221 ggtaaatgta gtgatgggtc ttgatatggg tggcgacgat tatataacaa aaccttttaa
    26281 attaaatgaa ttaatttcaa gaataaaagc attacttaga cgttataatg ttgcaagtaa
    26341 tgtgaatgaa ttaagttcaa ataatattac tattaaatta cttgaaaatc gtgtttttaa
    26401 aggtgaattt gaggtggaac ttactacagc agaatataaa ttattgtgct tatttatgaa
    26461 aaataaaaat attgtactta ctagaaaaaa catattggac aagttatggg atggaaatgg
    26521 tagctttata gatgacaata cattatcagt atatgtaaga cgattgagaa ataaaattga
    26581 agataatcct gaaaatccta agtttctatt gaccgttaga agaatgggtt ataagtggaa
    26641 tgtaattaag tgagggtatt aatatgaaga tattttctaa taaagatata aaaatatttt
    26701 ttttaattct tatatctatt ttagtaatgt ttattatagt agggcaaata gtggttataa
    26761 gtataactaa caattataaa aaaacgttga tagaacatga ttataatata gctggatact
    26821 tatatagtat aggtgttgat aaatctaaaa taccatcagc atttgttgca catgaagata
    26881 aatatatttt aaaaggaaaa gaaatactag atacagctgg atatggattt gatatagatg
    26941 ttagctttac accaagtgta aaattgtttc aaagtaaata tataaaaact atgtctatct
    27001 atttattagt attttttact atgattattt tcatactata tatgtatttt tttaggcaac
    27061 aaagtaagat agaagaggct agttataaaa ttgatttatt tatgaatgga aatataaatg
    27121 aaagactaaa tacatatgaa gaaggtagct tatcaaaatt atttagttca atagatgtta
    27181 tgtcgacatc tttaaataca catatattaa aagaaaaaca gagcagagaa tttcttaaaa
    27241 atacaatttc ggatatatcg catcaattaa aaactccact tacagcttta ataatgtaca
    27301 atcagatact acaagaagaa agtcataata gtgatgtcgt tgaagctttt gtacaaaaaa
    27361 gtcaaagtga actagaacgc attgaaacat tgattaagaa tttactaaag attacaaaga
    27421 tagattcaaa aacaatagtt ctcaataaaa ataatgttaa tcttaaaaat ttaattgtca
    27481 aaattttgta tagctttgaa actagggcag aaaaagaagg aaaggttatt tctataaatg
    27541 gttcagaaaa tacattgttg ttctgcgatt atgaatggat gtcagaggct ttaagtaatc
    27601 ttataaagaa tgcattagat aatacaaaag agaatgataa aataacgata gagtggtttg
    27661 agacgactat aaccaccatt gtttcagtta aagacactgg aaatggaatc catgttgagg
    27721 atattcatca tatatttaaa aggttttata gaagtaagtt ttcaaaagat aatcaaggtt
    27781 taggactggg attaccacta gttaaatcta ttgtggaaca acataatggt actatttctg
    27841 tagaaagtaa actatcacaa ggaagtacat tcaccataag ttttttaaag cttactaata
    27901 tgtaagataa aatacatctt gcagtaaggt atatagatta ttattatatt aatcaattag
    27961 aaaggtgaga gagaaaatgg atattcttgt ggttaataat ttatgtaaaa catatggaaa
    28021 tggatataca aaagtagagg cattgaaaga tgtatccttt acagtttcaa aaggagagtt
    28081 tgtgactgta gttggagaat ctggttcagg aaagagtaca ctattaaatc tcttaggagg
    28141 tcttgatact ccaacatcag ggagaatatt tattgatggt aaagaaatat gttcaatgaa
    28201 agaggaaaaa cagactattt ttagacgtcg taacataggg tttatatttc aggcatataa
    28261 ccttatacca gaactaactg tggagcaaaa tataatattc ccattgttat tagattataa
    28321 aaaaccagat actatttact tagatgaagt attgactatt ttaggtctta aagataggaa
    28381 aaatcatctt ccaaatcaat tatctggtgg tcaacaacag agagttgcaa ttggaagggc
    28441 tttgattaca aagccaagta ttatactggc agatgagcca acgggaaatc ttgacagtaa
    28501 aaatagtaat gatgtaattt cacttttaaa agtgactgca gaaaagtata atcaaaccat
    28561 tattatgata actcataata cgaatatagc ttctacagca gatagaatac tacaggtttc
    28621 tgatggtgtg cttacagacc tgggggtgaa tagagaatga gaagctactt agaattagta
    28681 ccacaatatg caaaagtaca taagaaaaaa aatagaacta caattttatg tattattatt
    28741 gcagtatgct tagttactgc tatctttgga cttgcagata tggctataca atgtcaaaag
    28801 atacaattta ttaaagatga tggaaattgg cacatagtaa ttagaaatat tgatgaaaag
    28861 tttatatcag aaataaatag tagagtagat atagctcacc atggtagggg gcaagtcgtt
    28921 gacagtggag agtttaaatc aaaggagatt ttgatacaag gtttagatga agaagtagca
    28981 aaacaattta acttagaaat aataaggggt cgttatccta ttggggagaa tgagacttta
    29041 atagatgagg atgcagttaa aagctttaat ctaaaaatag gagattttat caaaataaaa
    29101 ttagcagatg gtagaaataa aaattataag atagtaggtg aatattcatc aatatcaagc
    29161 ttaagagcta cagataaaca tggattaata ctttcaatat tgggtacaca agctattaaa
    29221 aaatcagaaa cgtacgatta ttattatatt acatttaaag aaaaggttaa tattagaaaa
    29281 gccacttctg atattaaaaa taccttcaat ttaagtgata aacaaatcgg taaaaatgaa
    29341 agattacttt ctattatagg tcaaggcgac tctgactttt ctaaaccact gtatttaaca
    29401 gcaggattac tttttgtact tgttttagta gcaagtatta tcatgatata taatagtttt
    29461 aacatgagtg taattgagag agttaaattt tttggtctgt tacgttgtct agggtcatca
    29521 aaacatcaag ttaaaaaatt tgttgtttta gaaagtttaa ttttaagtat aaaagctatt
    29581 ccaataggct tagtattagg aagtcttgta actattatat cttctatatt tcttaagtat
    29641 gttaatgagt tattcagctc aatgccaatt ttaaaagtta gttttattgg tattgcattt
    29701 ggaataattg tagggttttt aactgtaata ttatcagcta ttattccagc aaaaaaagct
    29761 tcccgtgtat ctccacttag cgctgtacgt gggaatctaa tgtccaaaga aattttaaat
    29821 aacgggaaga caagtaaagt tgcaaataca ggaagtttta aaattgagac ttctatggga
    29881 ttaaatcatg ctttttcaag aaagaaatct tttgtactta tgacttcttc atttgccatt
    29941 agtattatac tatttttagg ttttagtgtg cttatagacc ttatgtatca aggacttaag
    30001 cctatgaagc cagaagctcc tcatatatct attgttgacc ctaattttaa gtctgtttta
    30061 actaaggaag atattaatga aataagcaat atcaaaggag taaagaaagt atacagtaga
    30121 atgatgacgg atatagaagc tacatataat gggaagaaga caaaatcaca attaatttct
    30181 tatgaaaagt atcaatttaa ttgggcaaaa aaatatctat taaaaggaga agttaatgaa
    30241 aataaactac agtcagaaaa ccttgcatta gtagaagagg ggcatggatt tgatatagga
    30301 gataagatat atattaaaaa tagaaatgct gaaaaagagt tagaggtagg aggtatactt
    30361 tcctcaattc catttgaggc taaagataaa tatattggaa atattataac atctgaaaat
    30421 acttttacga atataacagg tataaagggt tatataataa tggatattca ggttgataaa
    30481 gatactcctg gagatttaat tgaaaatata agacgtgtag tagatgataa cttagaggtc
    30541 agagataaaa gacaaagtaa cttagaggtt aaaaattcgt tttatactat ggcaacattt
    30601 gtatatggat ttgtgtttat tattgcgatt ataagcatgt ttaatatcat aaatagcatg
    30661 aatataagtg ttacaagtag aaagaattac tatggtgtta tgcgtgcagt aggcatatcc
    30721 aacagacaat taagaagaat ggtaatagta gaggcaggtt catatgcaat gagtggttgt
    30781 ttattaggta gtatactagg attaatgtta cataggtata tgtttatatc gttagtaagt
    30841 tcaatgtttc atttagagtg gcaagtacct tttaatttac tacttatcac tgtactaact
    30901 atgataataa taactttatt atcagtaaga aaaccagtca aaaagatttg tgaattagat
    30961 ataatagact caataaatgc acaatgaata tagctgttga tttaaattaa aaaaacgaag
    31021 taaaaatgaa atagaaaata tttttcattt ttacttcgtt ttttatatta taaaattttt
    31081 gttatgttac aaaatttcta taaagatgat gtgaatctaa acattatatg acaacgtgag
    31141 catttaagtt aaaatatttt aaatatacta ttttagataa gttccttctt catcttgata
    31201 aattaatcct tttttcataa gcactccaag agctcgttta aaatttttct tacttgaatt
    31261 aaataatata gaaatatctt tagggtcaga tttatcatta aatctcatat atccatcaga
    31321 accctctaaa taacttaaaa tcttattttc taaagtatca agttcatcct ttctatttcc
    31381 tctaggagaa agaccaagtt ttccatcttc atatattttt atgacatgta aattttcaag
    31441 aacatcacca gcttttagtt cagtaaagta ctcattatga agtattacac cagcatattt
    31501 attgtctaca catatcattg cagaattatt agtttgaaat ccatatacaa ctccagttac
    31561 aatatctcct acattgtaag tatggtctgt taataaatat gaatctatat ctgtagtagc
    31621 agcaattctt ttgcttttgt ctaaatatat ataaaataaa tatttttcgt ctctaaatag
    31681 tggataagtc ttagctttaa aaggaactag tatgtcttta ggaagtccca tatctataaa
    31741 agtaccaatg tccgtattat caactacttt taaatgtgct acatccccaa ccttagctaa
    31801 aggcggtatt aaagttgcag cagttctgtc atcagaatct ttaaatatga aagcatttac
    31861 ttcatcacct atgtttattt cattttttcc gattaatcta ttatgaagaa gtatatcgtc
    31921 ttttgtgtta ttagtttgtc catccaaaaa atatccaaat tcagtttttc tttttactgt
    31981 aagtttatta aaatcaccta tgtttatcaa attaaaatca tcctctcatt ttatatttat
    32041 aatttagcca taatatactt atgtagtagt ttgtcacagt atctatatta gtattagtga
    32101 aatttacaat aaataatata gttatttatg tatttttcaa ttctctagct tcttatatag
    32161 tataaactcc aatttctttt ttgtctttag gttttactaa aatttttaca aaataagtta
    32221 aaaagtattg aaataaaatt tcttttaaag tataattggt atataccaaa attggtatat
    32281 accatatcga aaagtggtga ctatatgaaa atagtaaata aatctagtaa tataccattg
    32341 catactcaat taagttcaat aataagagag atgatagaaa ctggtgagct taaagagggt
    32401 gatgcaataa tgccagaaag agaactttgc aacatacaaa atgtaagtcg tatgacagta
    32461 aataaaacca ttgttggatt agtcacagaa ggacttcttt atagggtaca aggtaaaggg
    32521 acattcgttg caaagcaaaa aaagaagtat cagttttcaa atgtaaaagg ttttactgat
    32581 gttatgaaag aaaagggagt aaacataaaa acggatatat tatcctttga gatggaacta
    32641 ccagatgatt tagttaaaag aaagcttgga ataagtgata atactacaaa tatatataag
    32701 attgtcagac ttagatatac tgatggagag ccatttggat tagaaatagt atatctatca
    32761 gaagaaatgt gtaaaggtct aacaaaaggg atattggata attcttcatt atatagggtt
    32821 ttaaatgaaa aatatggcta taagatacaa aaagctgaac aagtaatgga gccagtaata
    32881 ctttcagatg aagaaagtaa gcttcttgaa acagatgaag gtgctttagc tcttaaactt
    32941 catagaaatt cctataatag agaaggtagc cctatagaat ataccatatc aatatttaga
    33001 acagataaat atcaatatga aatagtattg agtgagtagg tgaataatat gaaatgttta
    33061 attaatggaa agataatatt aaaaaatcaa atcttggaaa ataaagtatt agtatttgat
    33121 gaaaaaataa ttgatatagc tgatagtgta ccaaaagatt gtgaagttat agatgctgat
    33181 ggaaaatata tatctccagg gcttattgat attcatatac atggaaatat gggaaaagat
    33241 acaatggatt cgactgatga atctattgaa acaatttcaa aatcaattat gagacatgga
    33301 gtaacatctt ttcttccaac tacaatgact atggataaag aacatgttta tgatgcactt
    33361 gaagttatta aaaaagccca aaatagaaag cttgaagggg cacaagtatt aggagcacat
    33421 ttagaaggac cttttataaa tgagaattat aagggagcac aaaatgaaaa atttataata
    33481 aattcaaaat acgaatttat aaaagaatat aaagatgtta taaaagttat aacttatgct
    33541 ccagaaaaag atattgattt tgattttact agagaaataa aaagatgtac agatatagtt
    33601 ttatctattg gtcatagtaa tgcaaattat gaccaagcta aagaagctat aaatttagga
    33661 gtaactaata taactcacat gttcaatgca atgactggtt taaaccatag agacccaggc
    33721 gttgttggtg cagcacttac aactaatgtg tacagtgagc taatagcaga tacaatccat
    33781 ataaataaag atttatttca atttatacta aataataaag ggaaagaaag actgatttta
    33841 ataacagatt ctatagaagc tggtggtctt gaagatggca attattctct tggaggtcag
    33901 aaagtcatag ttaagggtaa tgaagctaga cttgaaaatg gagcattagc tggaagtgtg
    33961 ttgtctttaa ataaaatggt atttaacttc ttagataata caaaccttaa agtgaatgaa
    34021 gcaattaatt tagcttcttt aaatccagca acatctttgg gaataaatga caaaaaagga
    34081 agcttagaaa taggtaagga tgcagatata gctgtatttg atgagaattt agattgtaaa
    34141 atgacacttt gcttggggga cgtagtatat aaaaatatat agtatacaaa acaaacttta
    34201 aagacagtaa aaacaatata aaaaataata aattaaactt acattagtta aagtagtagt
    34261 taaaatttag gaggaataag atgagagtat tagtgtgtaa agattatgat ggaatgagta
    34321 aaaaagccgc tgaaatgata gcagctcaaa tagttttaaa accaaattca atactgggac
    34381 tagctacagg aagtacacca gtaggtatgt atagagattt agtaaaaaaa tacaatgata
    34441 atatagtaga tttttcagat gtgatgagct ttaatttaga tgaatattat aagttaccaa
    34501 tatctaatga ccaaagctat gattatttta tgaaagagaa tctatttaat catgtaaaca
    34561 taaagctaga aaatactcat ctaccaaatg gaatggctga tgatattgaa aaagagtgtc
    34621 tgaactatga agcttctata gatgcagctg gtggtataga tgtacaagta cttggaatag
    34681 gtcgtaatgc tcatataggt tttaatgaac ctgatactaa atttgcaaaa agaactcatg
    34741 tagttgaact tacagagtct acaatagaag ctaatgcaag attctttaaa tcaagagaag
    34801 atgttcctaa aaaagctgta agtatgggaa taggttcaat attgaagtct aagaaaatat
    34861 tgcttttagc ttcaggagaa gaaaaagctg atgcagtata taatactgtt tacggagata
    34921 taactccaga agttcctggt tctatacttc aacttcataa agacactata gttatagtag
    34981 atgaagcagc agcaagtaaa ctgaatccaa aagattataa attagtataa gaatagaatg
    35041 tagtttaagt gaaaaatagc tatcattgaa taatcaaatt tatttaatga tagctatttt
    35101 tatttaaatt ttttacttct ttttatttaa attagttatt attgtttaaa ttacttgctt
    35161 attacttttt acgaaaactc gttatcaaga agtatccgaa taagatgtta aaaattatta
    35221 tgtttagtgg gattctatca agcataataa aattacctat aattgggata ttaccaattg
    35281 gaaacataaa gaagaataca ggcatcagtg atagtaaaaa agatggaaaa caaagaagaa
    35341 gtaatcttgg aatatcaata taccaactac catccttttt gtattctcta atcaataatt
    35401 cagttcctag taacaaacca ataccaccag aggaaattaa attaataaaa atgtatcttt
    35461 taaagtcttg catataacca gtgtgaaatt gagtatagaa atcataattt aatttaacgg
    35521 ctaaaaaata tataagcaat agaatgatta aaaataaaag atatctaaga gcttttttca
    35581 ttttaaattc tcccttcaga aatactttat tcagttttat ttt
//
LOCUS       DAEPOG010000019        34324 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEPOG010000019 DAEPOG010000000
VERSION     DAEPOG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.019"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            259..663
                     /locus_tag="KR432_000825"
     CDS             259..663
                     /locus_tag="KR432_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0639379.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            687..1184
                     /locus_tag="KR432_000826"
     CDS             687..1184
                     /locus_tag="KR432_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639380.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            1187..1588
                     /locus_tag="KR432_000827"
     CDS             1187..1588
                     /locus_tag="KR432_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0639381.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            1606..2094
                     /locus_tag="KR432_000828"
     CDS             1606..2094
                     /locus_tag="KR432_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639382.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            2121..2876
                     /locus_tag="KR432_000829"
     CDS             2121..2876
                     /locus_tag="KR432_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0639383.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            2876..3739
                     /locus_tag="KR432_000830"
     CDS             2876..3739
                     /locus_tag="KR432_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0639384.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            3786..4265
                     /locus_tag="KR432_000831"
     CDS             3786..4265
                     /locus_tag="KR432_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBH0639385.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            4330..5637
                     /locus_tag="KR432_000832"
     CDS             4330..5637
                     /locus_tag="KR432_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0639386.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            5948..6088
                     /locus_tag="KR432_000833"
     CDS             5948..6088
                     /locus_tag="KR432_000833"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBH0639387.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            6600..7694
                     /locus_tag="KR432_000834"
     CDS             6600..7694
                     /locus_tag="KR432_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639388.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            7699..7899
                     /locus_tag="KR432_000835"
     CDS             7699..7899
                     /locus_tag="KR432_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBH0639389.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            7899..8816
                     /locus_tag="KR432_000836"
     CDS             7899..8816
                     /locus_tag="KR432_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639390.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            8813..9571
                     /locus_tag="KR432_000837"
     CDS             8813..9571
                     /locus_tag="KR432_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639391.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            9954..11027
                     /locus_tag="KR432_000838"
     CDS             9954..11027
                     /locus_tag="KR432_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0639392.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            11097..11360
                     /locus_tag="KR432_000839"
     CDS             11097..11360
                     /locus_tag="KR432_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639393.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            11566..12543
                     /gene="bioB"
                     /locus_tag="KR432_000840"
     CDS             11566..12543
                     /gene="bioB"
                     /locus_tag="KR432_000840"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH0639394.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            12771..13784
                     /locus_tag="KR432_000841"
     CDS             12771..13784
                     /locus_tag="KR432_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0639395.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            13826..14716
                     /gene="rbsK"
                     /locus_tag="KR432_000842"
     CDS             13826..14716
                     /gene="rbsK"
                     /locus_tag="KR432_000842"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH0639396.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            14764..15726
                     /locus_tag="KR432_000843"
     CDS             14764..15726
                     /locus_tag="KR432_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0639397.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            15780..17291
                     /locus_tag="KR432_000844"
     CDS             15780..17291
                     /locus_tag="KR432_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0639398.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            17275..18273
                     /locus_tag="KR432_000845"
     CDS             17275..18273
                     /locus_tag="KR432_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH0639399.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            18457..19719
                     /locus_tag="KR432_000846"
     CDS             18457..19719
                     /locus_tag="KR432_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0639400.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            19739..20482
                     /locus_tag="KR432_000847"
     CDS             19739..20482
                     /locus_tag="KR432_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH0639401.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            20655..22040
                     /locus_tag="KR432_000848"
     CDS             20655..22040
                     /locus_tag="KR432_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0639402.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(22377..23021)
                     /locus_tag="KR432_000849"
     CDS             complement(22377..23021)
                     /locus_tag="KR432_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0639403.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            23285..23899
                     /locus_tag="KR432_000850"
     CDS             23285..23899
                     /locus_tag="KR432_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBH0639404.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            24120..24260
                     /locus_tag="KR432_000851"
     CDS             24120..24260
                     /locus_tag="KR432_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639405.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(24490..24937)
                     /locus_tag="KR432_000852"
                     /pseudo
     CDS             complement(24490..24937)
                     /locus_tag="KR432_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            25707..26546
                     /locus_tag="KR432_000853"
     CDS             25707..26546
                     /locus_tag="KR432_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639406.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            26536..26979
                     /locus_tag="KR432_000854"
     CDS             26536..26979
                     /locus_tag="KR432_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639407.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            26990..27481
                     /locus_tag="KR432_000855"
     CDS             26990..27481
                     /locus_tag="KR432_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639408.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(27683..28411)
                     /locus_tag="KR432_000856"
     CDS             complement(27683..28411)
                     /locus_tag="KR432_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0639409.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            28536..29624
                     /locus_tag="KR432_000857"
     CDS             28536..29624
                     /locus_tag="KR432_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639410.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(30062..30910)
                     /locus_tag="KR432_000858"
     CDS             complement(30062..30910)
                     /locus_tag="KR432_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639411.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            31109..31474
                     /locus_tag="KR432_000859"
     CDS             31109..31474
                     /locus_tag="KR432_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639412.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            31497..33884
                     /gene="cadA"
                     /locus_tag="KR432_000860"
     CDS             31497..33884
                     /gene="cadA"
                     /locus_tag="KR432_000860"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBH0639413.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        13347 a         3595 c         6195 g        11187 t
ORIGIN      
        1 aatatgatac agataaattc aaaagttgat acaaaattta aaaatagcca aaaggctatc
       61 ttttttttac tttttttgat acagatttga tacaggttga aaaaatcttt tgatacatat
      121 cgaaataata agtttttaaa aaaaattgag taaaaagttt tgattaaatc ctgtaaaata
      181 gccatttatt attgatacac ataagttggc acggcaattg cttatataaa aagtgacaag
      241 aaaagtgagg tgaaattggt gataggaata atagttatga ctcatgggag ttttagtgaa
      301 gaaattataa aaagctgtga acttattgca ggaccagcag aaagaacagc agcaattaaa
      361 ctaaatagga atgataatat tgaagactta aataaaaatt ttgtagaaaa gttaaatgaa
      421 ttagatgaag gtgacggagt tttagtatta gctgacctat taggaggttc gccaagtaat
      481 gttgcaagtt taaaccttaa aaaaggtggc aagtttcatg cacttactgg agtaaatctt
      541 ccaatgctac ttgaagcttt aattaataga gagggaaaaa gtttagaaga gttagcagaa
      601 gcatgtattg aagcaggaca aactggtata aacaatatta ataaagttct tagctcaatg
      661 taatagaaaa aggagtttga ttaaaaatgt ctttagtaaa atattgcaga tgtgatgata
      721 gattaataca tggtcaagtg atatataaat gggttaagca tctaggtgta aaaaagatag
      781 tagtagtaga tgatgaaaca accaatgatg ttattgctaa aggtttgata aaaatggcag
      841 caccaaagaa tattgattta tcaatattaa ctgtaagtga atcaaggaga tatttttata
      901 ataatcaagc tgatgataac gtttttgtat taattaaaaa tttagatact gcaaatagaa
      961 tgatagaaga agggattaat atcaaaaaac ttattgttgg aagaatacct acaggaatag
     1021 gaaaaaagaa aatatctcaa aatgtataca ttaataaaaa agagtttctt ttaatagatg
     1081 aatttattaa aaagaatatt aatgtatcaa ttcaaatggt acctgatgaa gaagaagtag
     1141 aattaaacca aaatattaat cgctataaag gagagtttat ttagcaatga ataaatataa
     1201 ggtaattata ctgtgccatg gaacttgggg aaaagtctta gtagaagaaa caaataaaaa
     1261 ctttggattg tcaaatcaat atgaagtttt atcactaagt tctgaaaaag atgtttctgt
     1321 atttatgaaa gaggttgaag aagtagtaga taaagaagat gtgaaaatta taatatcaga
     1381 tttatatgga ggatctacat caagtgttgc aatagctgtt gctataagaa gaggaataga
     1441 tgcaataaat ggacttagct tacagacaat tttaatcgta gatgaagaat taagcaaaga
     1501 agataaatta catacattac cagaaagaat cgtcaaaaaa aataataacc tttgtgtgga
     1561 tttaattaaa gaatttaaaa aaatataaat tatgaggtga atataatggc aaaaataaca
     1621 ttagtaagag tagaccacag attaattcat gggcaagtta taacaaaatg ggtaaaaatc
     1681 gctcaagctc aaaaaataat aatagttgat gacttcttag gtcaagatga atttatggct
     1741 gacatatata aaatggcagc accatcagga gtagaagtag ttattttaac agcagaagat
     1801 gcgggacaag cattccaaaa caacacttta ggagataaaa atatatttat tctatttaaa
     1861 aatgttgata tggcaaataa ggcttataaa gctggactta aatatgaaaa gattcaatta
     1921 ggaggtattc caaatgaagc aggaaagaaa atggtgttta cagctgtttc attaggaaat
     1981 gaggatgtag aacaacttaa tgaacttaat gaaaatgggg ttgaaatagt cctacaagtt
     2041 ataccagagg aatcatctat gacatatgaa aatgcgttaa agaaatttaa ataaaaaata
     2101 taaattagga ggaattataa atggaatctg ttttgatgtt ggcaattgta acaggattat
     2161 ggtattggtt tgcagcaggt cttgcagggt ataccctttt ctcaacacta aagtctcctt
     2221 tatttatagg ttttagcctt ggattacttt ggggtgatgt aacaactggt atgattgttg
     2281 gtgcaagtat agaaatggtt tatctaggaa tggtcgcagc agggggaaat attccatcag
     2341 ataaatgtct agctgctctt attgcaatac cagtagcact tcaaactggt gttaatgcag
     2401 aagttgcagt atctattgcg gtacctttag gtgttatagg agtattagtt aataacttaa
     2461 gaagaactgg taatgctgta ttggttcata aggcagataa gtatgctgaa gaaggtaata
     2521 caaaaggcat ctggagatgt gcaacacttt attctttaat atttgggttt gttttaagat
     2581 ttccaatagt ttttgtatgt aacttttttg gagcagattt agttcaatca ttattagatg
     2641 ttataccaca atggttaatg aatggattaa cagtaatggg cggtatatta cctgcattag
     2701 gttttgcaac aacaatattt actataggta aaaataaatt cttaccaatg tttattatag
     2761 gattttttat ggttcaatat tttgaaatat caattactgc agctgcaata tttggtgttt
     2821 gtatagctct tttaattacc tttatgaaag aagacaaaag ggtaggtgaa gtataatgca
     2881 agaaataaaa aataaagaag ttaaaaatgg acaaggtcca actgaagtta ataagacaag
     2941 agtactcaca aagaaagatg taacaaaaac atacctaaga tggtggtgga cagcagagtt
     3001 atctaattct tttgaaagaa tgcaagcatt agcagtttgt gcaagtttca ctccggcttt
     3061 agaaaagtta tataagaaaa aagaagattt agtagatgca ttaaagagac acttacaatt
     3121 ttttaatact caagctatat ggggaggatt aattcatggt acagttttag ctatggaaga
     3181 agagaaagct actgaaggaa aaatcccagg agaagttatt tcaggtgtta agaatggtct
     3241 tatgggacct ttggctggaa ttggagatac tttagatttt ggtacatttc aaacaatatt
     3301 cttagcttta ggtgcttcat ttggagcaga aggaagtgtt ataggtgcat ttttcccaat
     3361 aatgtttagt atattactat tttgcgaagg ttattactta tttcatttag gatattcttt
     3421 aggtagagat tcaattaaga aaattctatc aggtggtatt gtcaataaga taattgatgg
     3481 tgcttcaatt ctaggaatgt ttatgatggg agcattatct gcaactacag ttaagctttc
     3541 aactccgcta tcatttgata ttggtggtaa agcaattgtt gttcaagata cattaaatat
     3601 gattgctcca ggtttattac cacttggtgt tgtattcttc gtatattggg gaatgaaata
     3661 caagaagtgg acaataacta agttattagt aattttagtt gtattagcat tggtaggatc
     3721 attcattgga atattctaat tcacagtgat taaaatatag aatttaattt taggaggaga
     3781 aagaaatgtc agaaaaaatg tatacagaaa aggaatattt agaaggaatt agaggagcaa
     3841 caggagaaag agcagtatgg ttctatcttc ttatgaaaga agctgaaaaa ttaggagcta
     3901 atccagatga tatatgtaaa gaagcaatat atggatttgg taaaatgaga ggtcaaaaat
     3961 ataacgtagc agatactcca ggaaaaatgg cagaaatgtt atataacagt aaaggacaaa
     4021 aagtgtttga aatggaatta gtagaaaata cagatgagaa tggcgtttta aaattccatc
     4081 actgtccatt agatgctgct tggaaagaat atggattaac taaagaagaa agaaaagaaa
     4141 tttgtagatt agcttgttat ggggattatg gaagagttga ctgtgcacaa ggtgttaaat
     4201 tagattttgc tcaaaaatgt gctcatgatg atgaagtttg tgaattagta tttacaaaaa
     4261 aataattttt aatgctaaaa gcctgttctt taaggacagg ctttaaaaag atagaaagta
     4321 ggttgattta tggatataag cagaagtgta gataatagtt taaatagaac catagaattt
     4381 ctaaaagaat tgataaaaat tgattcacaa caaggtgaac ctatttcaca atgtcctttt
     4441 ggtataggac ctaaaaaatc tttagataaa actttagatt actgtgctag tttaggtttt
     4501 agtgttaaaa atattgataa ttatattgga tatgcagaaa ttggagaggg agaggaatta
     4561 atagggattc caatgcattt agatatagtt ccaccaggag aggggtggag tgtagaccca
     4621 ttttcgggag cagtaattga caatattatt tatggaagag gtgtcataga taataagggt
     4681 gctgtatcta tgcttataca tgtattgaaa aacatagagg atatgtatcc tacaattaat
     4741 aaaagaataa gattaatttt tggtacaaat gaggagacag atatgaaatg tatcaaatat
     4801 tacttagata aaggtgaaga aattccaagt atgggattta ctccagatgc tatgtatcca
     4861 gtggttaatg gagaaaaagg aagggttcat ataaggatag aaaaagaaat taaaattgac
     4921 aaaagtaaac cttacataat agtaagggga ggaactaaag aaaatgttgt tccatctcat
     4981 tgtactgcaa aaattattaa tggaattatt tcagagttaa ctactaaagg agtagctgta
     5041 catgcaagta atcctgaaaa gggagaaaat gctatatcta aaatggtaat aaagatagtt
     5101 gaggataata tggattttca gcatagagaa gatatagaat tagtttcaaa atacctttgt
     5161 agtgattatt atggagatgc attggggatt aatcaatatg atgaggtatt caaaaacact
     5221 accttaaatt taggaatatt aaaagtaaat gaggaaaaaa tcgtatgtga gttagatata
     5281 aggtatggta aaaatatagt tttaaataac ataatagata gattcaaaaa ggttttttgt
     5341 aatggttgga aaatagaggt tattgctcat aaagacttac attatgtgga tgagagtaat
     5401 ttagtactta aaaaattatt agaagcctat gaagaagtaa cagatgaaaa tggatataca
     5461 attgctatgg gtggtggcac atatgcaagt tggtttaaag atatggtggc gtttggacct
     5521 aagttcttag cgtataaaac tggtggtcat ggagtagatg aaagagtacc aataaatcat
     5581 atacgaaaga atatggagat atatactttg gctttaataa aactattaga attgtaaaag
     5641 aagaagagac tttatgtgca aatttacaga tatcttaaaa agagagataa aacgagttca
     5701 actttattta aatgttcaac accatcaaat ggggctgtct caaaatagag ataagcctct
     5761 tgcttattat tgaaatgatg aaaatatatt ttacttcata caaaaaagag tgccttatga
     5821 agtaaaaagt taattttgag acaccctcat ttgatgatgt tgaagagaaa ataagtatga
     5881 aattattaaa aaaagatgat tttataaaag ttaaggttga aaataaaagt taattgaatg
     5941 gaattagata aatggagaag gctgtatact atctaataaa ataacaaaag agtgttataa
     6001 aactggttat atgattagag aaaagtctat tgatacttat ccagatagtg gacggggatt
     6061 tatgacttgg gatgaaaatg gagaataata aaaattatag tttatacaat ttaataagat
     6121 atatgtacaa gcagttaatc taaatgattg actgcttttc ttttatagat ttttatataa
     6181 tagacaatta ctaatacatg atagttgcct gttataatta gaggaataga tacgaataat
     6241 aactttataa aagaattaaa tggaaaattg aagagttata ttcaggattt taaaaagaat
     6301 atgaaatgag aaatgaaaat aaggtgttta tattaaaaga ggttttacac aaaatactta
     6361 aaaacgtgat aaataaaaac tgtatttaga taaattaact actagtttta tatataatat
     6421 ggattttgtt tacaaagttt acatatttca caaaatattt ttaaaatata tttaaaaata
     6481 tattttatat acttgttcgt ttgtttctct gtggttgatt ttttcgtcaa aagtcaactt
     6541 caaatcgctt gttagcttta catgtttaag gatataatta tacataagga gggattgaca
     6601 tgaatgaatt aaacatcgca aaaacgctga ttttaaagcg taaagaaaaa ggaattacac
     6661 aagatgaact tgcaaattat ataggcgttt ctaaagcttc tgtatctaaa tgggaaactg
     6721 gacaaagcta tcctgacata acctttttac cgcaactcgc gacatatttc aacattaccg
     6781 ttgatgagtt aatatgttat gaacctcaaa tgatgaagga agatattaat aaactgtata
     6841 ataaactgtg caaagacttt acggttaaac cgtttgatga agtcatggat gaaataagag
     6901 aaatcataaa aaaatattat tcatgttttc ctttgatttt tcggatgggt cttttaatag
     6961 ttaatcacta tgatattgta gatgaaaaaa agcgtgaact attaattagt gaagcattag
     7021 aaatatttat tcgtatacag gaaacttgta atgacataga tatttgccgt caagcaaaaa
     7081 gtatggaggc aacttgttat atattgttaa atcaacccat acaagtgatt gatttattgc
     7141 aaaacagcaa ttttcccatg ataaatgaat ctatacttct tgcacagggg caaatgatga
     7201 atggtcaaat ggatgaagca agagaaacat ttcaacttgg tgcatatcag aaccttatat
     7261 cattagtcca aaatctagtg ggtattttgc aaaatgcaga taaattacaa atgaaggaaa
     7321 tagaaagacg tattctagca atttcagata tatttgagct tgatacatta tctcctgcta
     7381 ttatgctttc ttcatattta acacagacac aaattaattt aatacatggg gataatgaag
     7441 gagctattaa atcattacga aaatatgtag atttagcaac acgtgatata tacccaatta
     7501 taatacatgg ggatgacttt tttaataaac ttgacagatg gatttctgaa attggaacag
     7561 gtattacacg tgatgataca atcgtaaaag caggtattgt tgctgctatt aaaaataatc
     7621 caatgttttc tttattaagt gaaaacaaag aatacaaatt tcttatagaa aaattatctt
     7681 tattggagga ataagattat ggaaaatttg atggaatatt taccactttt gattcctgtt
     7741 attattttag atttaatact tataattact gcacttgttc atgtattgag acatccaaat
     7801 tataaaattg gaaataaggc aatatggatt attgtagtac tgtttattag tctaatagga
     7861 ccaatcttgt attttacaat aggaagaggt gaagaatgat gaatattgtc tcaataaatg
     7921 gattatcaaa aggttttggt aatcgaaaga taatagataa tctaaatttt actgtaccag
     7981 aagggtcagt ctttgggttt gtaggtaaga atggtgcagg taagactaca acaatgaaaa
     8041 tggtacttgg gttacttgaa ccagatagtg gaactataga tgtttgtggt gagaaagtga
     8101 catatggaag aacaagttct aatcgtcatg taggttatct gcctgacgtt ccagagtttt
     8161 acaactatat gagaccactt gaatatcttt ctctttgtgg tgaaattacg gggctttcaa
     8221 aaaaagaaat aaagataagg agtgaagagt tgttatcact tgtggggctt ggcaatgaaa
     8281 agagacgaat aggtgggttt tctcgtggaa tgaagcaacg acttggtata gctcaagctt
     8341 tgctttcacg tcccaaatta cttatttgtg atgaaccaac aagtgcactt gaccctgttg
     8401 gtcgaaaaga aatattagat attatgctta aaatcaaaga ctcaacaact gtaatatttt
     8461 caacacatat actatcagat gttgaaagaa tctgtgacca tgttgctata ctaaacaaag
     8521 gaagcattgc tcttagcggt acactttcag aaataaaaag tatgcacgga aaggatagac
     8581 ttttactgga gtttgcaagt aataatgaaa ttcaaaagtt taagtctagt ggtggcatta
     8641 aatccttact aaaagattca gaagaaacta atatggaaat tgttttgcat ggtaaagata
     8701 taaaagcaat acaaaaaaca gttatatcta cactcgcaga aatgaatctt tgtccagtaa
     8761 agatggaact aatagaattg tcacttgaaa atttgttttt ggaggtagta aagtgagaga
     8821 atacatagca tttacaaaaa aagagtttaa ggagaacata agaaattata aattacttag
     8881 tttgactatt ttatttttag tatttggaat aatgagtcct ctttctgcaa aatttatgcc
     8941 agacttgatt gctcattttg ctccaacttt aaaagttact actgcaccaa ctgctttaga
     9001 ttcttggaca cagttttctg gaaatatttc tggtcttgga acgagtttaa ctctaattgt
     9061 tttttgcaat attttatcaa acgaatattc taaaggaaca ctcgttatta tgttgactaa
     9121 agggttatca cgttcatctg tagtattgtc taaattttct attgcagtaa ttattatgac
     9181 aattggtttt tggttgagct ttttatgtac ttatggatat acaatgtatt tttggccaac
     9241 tgcaaatctt aatcatatta tattttcagc atttaactta tggttaatta gtattatgta
     9301 tatttccata ttaattcttg ggtgtgtact atttagacca gcatttgcaa gtgtgttact
     9361 tgtattagca gcaactgcta ttttgagttt gatttctatt cctaaacaga ttgcacctta
     9421 tacgcctaat ttcatattat caaaaaatat agatttagtt tcaggtaaag tgacacctcc
     9481 agaatttatt attccaatca ttgtaacaat tgttatttcg atagtatgtc tgttattagc
     9541 tgttgttttg tttaataaaa aagcagttta aggatatttt tattataaat acaaatagtg
     9601 ctgtattgtg ggaaaatgtg tttagtattg tagaagaata tatttaagta aaaaagaggc
     9661 tgtctcaaga tagagaaata agcctctttt ttattttagt cctcctcact atactttata
     9721 ataattaagt tattacctta aggaactatc taaaaaaaga gatatagatt ataatctaat
     9781 gcttatcagt tatttgaggt gtttagtata tagagtctaa agtagtgaag gactaaaata
     9841 atattgtttt cattaaataa agataagaat ctttaattgt ggtaaaatat actaaaaaat
     9901 cattttaaaa gtgataaaat tattttaagt aaatatttta aaggggtgta gatatggaaa
     9961 agtcaaaagt atatttttgt gatttatact caaattcgca aaataaaaac gtaccaaaca
    10021 atgtaagaag attatttgat gaagcaggat tcaaagattt aatagaaaag aatgaccaag
    10081 tcgcaattaa acttcacttt ggagaaaagg gaaatactac atatatgagt cctgtggcag
    10141 ttagacaagt ggttgataag gttaaagact gcgaaggaaa gccattttta acagatacaa
    10201 atacactgta tacagggagt agaacaaatt ctgtagacca tcttacaact gctatagaaa
    10261 atggatttgc ttatgcagtt gtaaatgcac cagtaataat tgcagatgga ttatatagta
    10321 gaaattatga gaatgtaaaa atagacaaaa aacattttga aagtgtaaaa ataggtggag
    10381 aaatttataa ttcttctgca atgatagtaa tgagtcattt taaaggtcat gaagcagcag
    10441 gttttggagg agcattaaaa aacttagcta tgggatgtgc aagtgcagca ggaaaacaaa
    10501 tgcaacattc tgatgttact ccagtagtaa aggaaaagaa gtgtgtaggt tgtggaaagt
    10561 gtgttaattc ttgtcctaca aaagctataa gtatagtgga taaaaaagca gttatagatt
    10621 ctgatgtatg ttatggatgt ggagaatgtc caactgtctg tccaactaga gcagtaacta
    10681 tacaatggga aagtgactca gatgtgtttg tcgaaaagat ggcagaatac gcttatggag
    10741 cagtatcaaa taaaaaagac aaggtagggt atataacttt tgttatgaat gtaactccac
    10801 tttgtgattg tgtaccttgg tcaggtagac caattgccca tgatatagga atattagcat
    10861 caacagaccc tgtagctatt gaacaagcat gctatgattt gatttgtaag gaaatgggtc
    10921 atgatgtttt caaacatgaa catcctcatg taaatggaac tagaataata gactatgcat
    10981 gtgaaatggg tatgggaagt aaagaatatg aattaataaa attataagaa tagtagcact
    11041 taaatgcata taattattgt aagagaaatc taaaagtatc aaaggagtat gcatttatga
    11101 aaatttacta tgagaaggaa aacagcaaga aggctaatgt aaacaatatt ccaagtgtat
    11161 ttgcgtttaa ttttctaaga aactttttga ctagcaaaga gtatgaggaa actagaaaag
    11221 aatattttat aaataatcta acaaaaagtc aggttaatca agccatgaag gatttaaaat
    11281 ggctatttag agaatatgaa ggcttagagg ttattacaat ggaaagcttg gatggagtta
    11341 agacaaggat agttttataa accagtaaga attatattct aataaaaaat actgctttaa
    11401 taataatatg tataatctat atactaatat tattttgaag cagtattttt ttgtgtatag
    11461 taaacttgtg tctaaaataa agttgaccaa taaatgttat atatgcaata ctatatataa
    11521 taagtaaact acataaaaat attaagttga caaataggag gatatatgga aaaatacata
    11581 ataaaattaa aaaataaagt attgagagaa aaagaaataa gttatgaaga agctcttaat
    11641 ttaataagcc ttgatacaaa taataaaaac gattttgata ttctattaaa atctgcaaat
    11701 gaaataagag aatattttat gggaagaaaa gcagatttat gtacgataat gaatgctaaa
    11761 tctggcaaat gtagtgaaga ctgtaaattt tgtgctcaat catctcatta taaaactggt
    11821 gtagaagaat attcattatt agattacaat gaaatcttaa atagagctaa agagatggag
    11881 agtaaaggag tgcatagatt ctcattggtt acaagtggta aaggtatgtc aggaaaggag
    11941 tttaataata ttttaaatat ctatgaagga ttgagggaaa atactaattt aaaattatgt
    12001 gcatctttag gtattataga ttatgagcaa gctaaaatgt tgaagtcagc aggtgtgact
    12061 acatatcacc acaatgttga gacgtgtaga gataattttc ataatatatg tacaacacac
    12121 acatataaag ataggataaa aactataaaa gatgctaaaa aagctgggct agatgtatgt
    12181 gtaggcggta taattggaat gaatgagagt gaagaacaga gactaaaaat ggtttttgaa
    12241 atacgagaat taaatgtaaa atcatttcca ataaacattt taaatcctat taagaatacg
    12301 ccaatggaaa attatgatgt attagaacca atggaaatac taaaaacaac agcagtattt
    12361 aggtttataa ttcctaatgt gtacataagg tatgctggtg gaaggctatc attaaaggga
    12421 tatgacaaag ttggatttaa tgggggagta aattctgcaa ttgttggtga ttatttgaca
    12481 actgttggaa gtgggattga aaatgataaa aaaatgatta tagagcaagg atttgaactt
    12541 taataaatac tacttaaatt aattaatata cttttagata attataaaag tatattaatt
    12601 ttttttgcag attaatttaa atattaaaat tgaattataa gttgcaatat tttgaaaaaa
    12661 gggaaaaata tcaaaattta tataaaatgg ttgcaaatgg gaaaaggatg tgatagtatc
    12721 taggtaaccg gttaagtaaa acggtttcct aaattttggg ggtgaacttc ttgagtataa
    12781 ctataaaaga aattggcgaa ttggctggtg tttctaaaac aactgtgtca aaagtcatta
    12841 ataataaaga tgaaaatata agtcaggcaa caagagaaaa aattttgaaa ataatgaagg
    12901 aaaaaaatta tgtaccaaat aaattagcac aaagtttagt aactaaaaaa actaatacta
    12961 tagggcttct tataccagat atacgaaatc cattctttac agatgtatca agaggtgtag
    13021 aagacaaagc taatgaagaa ggctacaata taattttatg taatacagat gaagatgcta
    13081 aaaaagaata tgaaggaata agaacactat cagagaggat gatagatgga attatatttg
    13141 cagcatcttc aaatacaaac tggaaagaag caaattataa agatataaaa ataccaactg
    13201 tcttgattga taagaagata agtatgaaca aagaaacctt aaaaggaatt gttaaaataa
    13261 ataactttga aggggcctat atagctacaa aacatttatt agatattgga aataagaaaa
    13321 taatatacct aagtggtcca cttcaaaatg aaatagcagt agatagatta gaaggatata
    13381 aaaaggctct aatcgagtgt aatttaagct acaattctgc ttatgtattt gaaggaaaat
    13441 ataagattga atggggacaa gaatttataa aaaatttaga aaagatagat tttgatgcaa
    13501 tattttgtgc aaatgatttg attgcaatag gtgtcataag aggtcttaaa gaaagagggt
    13561 taagtatacc aaatgatgta agtgtcgtgg gatttgatga tattcaaaca tcaagtctaa
    13621 taagcccttc tcttaccaca gtaaaacagc cgtcttatga tataggatat aaagccagtg
    13681 aaatattgat aaattgttta agaggagata aaaaagaatc atttgatgaa ttgattttca
    13741 aaccagaatt agtcataaga gattcaacaa aagaaagtga ataacagcac gagagacata
    13801 aatagatatg taaaggagta atgtaatggg aaacatagtt gtcataggaa gtgtaaacat
    13861 ggacatggta tgttctgtag ataaaagacc agaaaaagga gaaacagtat taggtaatag
    13921 tttttttaca tcacctggag ggaaaggggc taatcaagct atctcagctt ctaaactagg
    13981 agcaaatgta aaaatgatat catgcatagg ggaagatggc ttaggagagg agttaataag
    14041 aaattttaga acgaataaag ttgattatag tttagtatcc agaaataatc acaaaagttc
    14101 tggtgttgct gttataacat tatgtgaaaa tgataatagt attgttgttg taccagggac
    14161 gaatgagcta gtagatatag aattaattaa aaagaatgaa gaagagataa agaatgcaga
    14221 tatagtattg ctacaattag agattccatt aaaaacaata aattatgtag tgaatttctg
    14281 ttttgaaaat aggattaagg ttttattaaa tccagcacca gcagtaaaac taaatgaaga
    14341 tataatagaa aaagtaactt acttaacacc aaatgaacat gaatataaga tagtttttga
    14401 cacaaatgaa gggatagaag aagtattaaa aaaatatcca aataaacttg taataacaga
    14461 aggaaaaaat ggagctagat tttatgatgg tgaggaaatc aagcatgtat cttgtataag
    14521 tgttgatgtt caagatacca caggagcagg agatacattt aatggagcat tggcagtggc
    14581 tataacagaa gggaaaaaat tatatacagc agtagaatat gcagtagtag tatcaggtct
    14641 ttctgtaact aaactaggtg cacaatctgg tatgccttat aaagaagatg ttgaaaaata
    14701 tttaaataat aaataaaaat ctaatttgtt caaatttaaa actattaaaa atggaggtta
    14761 attatgagag tgagaaaaat tttatcttta ggaattgctg gaatactagc aataggaatg
    14821 ttaacagggt gttcaatgga aggaccaagt aaatcagata ataaaggtgg ttcagacaag
    14881 aaagatttaa ctattggagt atctacaata actcttcaac atcaattttt tatagatatt
    14941 gatgagggaa taaaagaaaa agctaaggaa ttaggtgtga aagttatagt aaatgaccca
    15001 gaccaagatg tagctaagca aacatcagca atagaagatt ttatacaaca aaatgtagat
    15061 ggaatgatag tacttggaac tgataattcg gctatagtac cagcagtaga aggagcattt
    15121 gaaaaaatgc cagtagttac agtagatgct gtattaaata ctgaaaatat tacaagttat
    15181 gtaggaacag ttagttatga tgctggtaaa aaattagggg aatacactaa aaaatacata
    15241 gatgaaaagt taggtggaaa gtcagaaata gcaatagtga cagatttaaa atctcaaata
    15301 caaatgcaga gaatagatgg atttaaagat gcgttaaaag gttcagcaaa tgttaagata
    15361 ttaaattcac aaccaggata tgacagagaa gaatctctta atacagtaga aaacttaatt
    15421 caatctaatc cagatgtaga tataatatat gcaacagctg aaaatagtgt actaggagct
    15481 aaagctgcac ttgaatctgc aaaaaataag gatgttaaga ttgtaggatt tgacttaact
    15541 gaagaagcat caagtggaat aacagatgga actattcttg ctatgattca acaacaacct
    15601 aaagagatgg gacgtttagc tgtagaagca gtagtaaaag ctataaaagg ggaaaaggta
    15661 gaaaagaata taccagtacc agctttactt tatgataagg aaaatataaa agactttaag
    15721 aattagtaaa ataaaatgag attatttgaa agaaatccta tttaaaaagg agtgtcatta
    15781 tgagtaatat aattctgaag ttaagtaata ttgctaaaga atttcctggt gtcagagcat
    15841 tagataatgt taattttgag ttatttcatg gcgaagttca tgcactactg ggtgaaaatg
    15901 gtgctggtaa atcaacaatg ataaagattt taactggtgc acattcgaaa acttctggaa
    15961 aatttatttt tgaaggcaaa gaaatagaga atatatcccc agatatctct aaaaagatag
    16021 gtataaatgc catatatcaa gaactaacag tttttgacga attaacagta gcacaaaata
    16081 tttttatggg aaaagaaata aatggcaaag tcttaaccaa tgacaaaaaa atgaatgaag
    16141 aggctaaaaa gattttcgat aatatgggaa tagatataaa tccaaatagt ttagtcaaag
    16201 agcttagtat agctcaaaaa caaatggtag aaatagcaag agttttatca tcagaaacta
    16261 aagtattaat aatggatgaa cctacttctt caataagtaa aaaagagact gaaatacttt
    16321 ttagacttat aaatgattta aaagagagtg gagttagtat aatatatata tcccatagga
    16381 tggaagaact ctttgagatt tgtgacagaa taactataat gcgagatggg aaaacgattt
    16441 cgactttgaa taccaaagat gtatcgtctg aggaagaatt agttaattta atgatagata
    16501 gaaagctaga ccaatttttc ccaaaaagaa aagtagaaat aaaagaagaa attatgcgag
    16561 tggaaaatct aactaagaac aatgttttta atgatattag ctttaatata agaaaaggtg
    16621 aaatattggg aataggtgga ctagttggaa gtaagagaag tgaaatagta gaagctatat
    16681 ttggacttag aacatatgat tctgggaaaa tatatttaaa caatgaagaa gtaaagttta
    16741 aaactccatc agatgcaata gaaaatggac tgggattaat aacagaagat agaaaaggta
    16801 ctggattgtt tttacaaatg agtgtcaaag aaaacacaac tatggctggg ttaaaaaaga
    16861 tatctaagtt taaatcaatt atagatagaa aaaaggaaaa agagatttta gaaaaataca
    16921 tagaagcttt aaagataaaa acaccacata tgaatcaagt tatacaaagt ttaagtggtg
    16981 gaaaccaaca aaaagctata atagcaagat ggcttttatt acaaccagat atattgatta
    17041 tggatgaacc aaccagaggt atagatgtaa atgctaaggc agagatatac aatctaatgg
    17101 gagatttagt ggagagtggt gtaagtataa taatgatatc ttctgaaata ccagaattaa
    17161 tatcaatgag tgacagaata atggttatga gagaaggtca tataagtgga tttttagaag
    17221 gtgaagaaat ggttgaaaat aatgtattaa aattggcttt cggaggtaaa attaatgagt
    17281 tcaataacta agcataaaga agaaaaatct tctccattat tgacaaaatt aacaagtgta
    17341 tttagagacc aagggagtgc agctgtagga cttgtaataa tttttataat aatgagtgta
    17401 gcttcatcta actttttaac tttagataat ttaattaatg ttggaagaca aatatcaata
    17461 aatgcaatac ttgcagtagg tatgacattt gtaataatta caggaggaat agatttaagt
    17521 gtaggagcag taattgcttt agtaggtaca ttttgggcta caacagtagt gaattataat
    17581 gcacctatat gggtaggtat gatacttgca ttagcaatag ggacaatatt aggagtcata
    17641 aaaggtgcaa ttatttcaac acaaaaacta cctccattca ttgtaacttt agccatgtta
    17701 acgataataa gtggggcaag ctttgtattt acaggaggaa gacctatatc agtaaataca
    17761 gatgcattta agatgctcgg tagaggatat attggaccaa taccaatacc agttataatt
    17821 atgattatag ttgtaatagc aggacatttt ctattaaaaa gaactaattt tggacgtcat
    17881 gtacatgcag taggtggaaa tgaagaggcc gctagattat gtggagtaaa agtaaataaa
    17941 gttatagtaa aagtatacgc actagctgga ctactaacag cattagctgg gataatacta
    18001 tcatcacgtt tggcatcagg ttcaccaact gttggagatg gagcagaatt ggatgctata
    18061 gcagccgttg tgcttggtgg tactaatatg atgggtggtt ctggttctat agtaggtaca
    18121 tgtataggtg tcggaataat aggaatatta ggaaatggat taaacttatt aagtgtatct
    18181 tcatataatc aaatgattat aaaaggtttg gtaatgttat ttgctatatg gataaataat
    18241 ataaaattaa aaaaatcagc taaatctaaa tagtaattaa attttagctt ataaactata
    18301 aaaattatta agtttttgta tatattttta tactttaatt tgcatacatc tttatacatt
    18361 gaatgggaat atgctaagtg aaaaagttta ctatggctta aatagatgag attagacttg
    18421 aaattttaaa tgtaaaaata aatcttagga ggaaatatga aaaaaattct cttagataca
    18481 ttaaatagta aaaaacaaga atacatagat tatttaaaag aacttgtaag tataaagact
    18541 gaagatgttg gtcatggaat actaggtgga tttgaaaaag aaggacagga atatattgaa
    18601 aaacttgcaa attacatagg ttttagtgta gatagacaag aaatgagcga agaattaatt
    18661 aaaaaagcaa aaaatatata taaagaagga aatctaggtc acaattatca agatagatat
    18721 aatttaattt gtaagtatag tgatgattta cctggtaaga caattgtatt taatggtcat
    18781 gtagacacta tgccaccagg tgatataagt aagtggaaat ataatccata tcgtgctaca
    18841 gaagataatg gaaaacttta tggtcttgga actgctgata tgaaatctgg acttatagca
    18901 tcaatattag ctgtaaagct tataaaagat agtggtctaa atgtacctgg taatgtaaaa
    18961 ataatgtcag tagtagatga agaaggtggc ggaaatggta ccataaatgc agttatgaat
    19021 ggtattggtg gagattgttg cataatttgt gaaccatcag aacaaaactt aatagttgca
    19081 catatgggat ttgtattctt tgaagttgag gttaaaggtg tgtctcttca ctgtggtagt
    19141 aagtgggagg gtgtaaatgc aattgagaag gctatgttgt tgcttcaaga tataaaagag
    19201 ttggaacata actggcttat gatatataag catccactat taccttcacc tacaataaat
    19261 ttaggtgtaa taaatggtgg aacagcaggt tctactgtgc cagataagtg tgtatttaat
    19321 ttatgtgtac acttcttgcc aaatataatg agttatgaac aagttgtaaa tgatgtaaca
    19381 aatgtcataa tgacaagggc taatggagat ttatggctta aagataataa accaaatata
    19441 aacatatatc aatcagggtt aggttttcag atggacaagg attctgattt tgtagtgaat
    19501 gcacataaaa tactagaaga aactttagga aaaaaattag agataaaagg ttcaacagct
    19561 ggcaatgatg cgagattaat gaaaaattta gctgaaatac caacacttat attagggcca
    19621 ggttcaatag aacaatgtca ttcaattgat gaatatgttg aaattaagga gtatttagat
    19681 agtatactta tgtatgcaag tctaatatta aacttataaa agtaggagga atatataaat
    19741 gaaaaaagta ttaatagtag gggaatcttg ggtaaaaaat ataactcata taaaaggatt
    19801 tgatacgttt gttacaactc attatgaaga agctgtaaag tggttgaaag aagcaataga
    19861 atctggagga tatgagacag ttcatatgcc tgcacatgta gcagcagata gttttccgta
    19921 taaattagaa gagttaaatg agtatgactg tataatatta tctgatatag gttctaatac
    19981 gttcttatta tctaatagta cttttataga ttgcaatagt aatccagata gacttgaact
    20041 aatcaaagag tatgtaaata atggtggggc gttaatcatg gtaggtggat atatgtcatt
    20101 tacaggaata gatgcaaaag ctagatttgg agaaactgct atcaaagatg tattaccaat
    20161 aacaatgata gataaagatg atagagttga aaaaccagca ggaataattc cagaagttat
    20221 tgactctgaa catccagtat taaaaggtat accaacagaa tggccaaagt ttttaggata
    20281 taacaaaaca gtagctagag ataattgtcc tgttttagca actattggag gagacccatt
    20341 tgtagctgtt ggcgagtttg gaaaaggaaa atctgctatc tttagttctg actgtgcacc
    20401 tcactggggt ccaaaagaat ttactgattg gaaatactat aataaattat gggttaatat
    20461 gcttgattgg ttaacatgct aaaaatttta taaaacttga aaacctctta cttgataaaa
    20521 tgaaacttac aagtcaagag gttttattgt ataaaatggt tgtatggtag aaaatatttt
    20581 taaatcagtt aaaattagtc attttggctg ataatcgata agttattaga aacagggagg
    20641 tttatataat taatatggat aatttaaata aaaagttttt gatggaatat atagatgaga
    20701 tacagggaga attaagagat atatcagata agatatggaa aaatccagaa cttcagtata
    20761 aagaatacta tgcttcaaat ttacaaaaag aatgtttaaa aaaacatggt tttagtatag
    20821 agggattaga aggtatggat acagcatttg tggctagtta tggagaaggt agaccagtta
    20881 tagcagtttt gggagaatat gatgctttag atggtttgtc tcaaaaagta tccactacta
    20941 aagaacctat tgtagatggt ggtgcaggtc atggatgtgg acataactta cttggtacag
    21001 gttcaatagg agctgtaatt gcagtaaagg aattaatcaa actaaaaaag ctgaaaggga
    21061 ctataaaata ctatggatgt ccagcagaag aagatttatc tggaaaagta ttgatgataa
    21121 aaaaaggttt ttttgatgga attgattgtg cttttagttg gcatccattt gatataaata
    21181 ctccgataag aatcccaaca cttgctaatt actctgtgaa atttagatat aatgggatta
    21241 gtgctcatgc agcacaagct ccttataacg gaagaagtgc acttgatgca gtagaactta
    21301 tgaatatagg ttgtaattat ttgagagaac atatatttga taattgtaga gtacactatg
    21361 taactactaa tggtggaaaa atgccaaata tagttcctga ttttgcagaa gtgtggtatt
    21421 acataagagg agttaaaatg gagcatgtaa gagatgtatt tggtaggatt gttgatatag
    21481 caaaaggagc agcattaatg actggtacta ttatggaata tgacattata agtggagtat
    21541 atgactatat tccaaataca atattaacag atattctatc acaaaatatg aaattagtag
    21601 gtgtgcaaaa ctataatgaa gatgactact attttgcaga taaattagct gagacagtgt
    21661 ctatagataa aagaagtagt gtttctagtg tattaagtgg aaataaggat attactaaga
    21721 tgaatttaca tgatgaggtt acagatgata catttactca taataactgt atatcattat
    21781 cattggatat tggagatgtt agctacataa ttcctacagc acaatgttct tgttcagttt
    21841 ggcctattgg tatttctgcc catacatggc aatcttgtgc cagtgcaggt tctgatatgg
    21901 gatttaaggc aatgttattg gcatctaaat ctatatcatg ttcaataaat gatgtaatgc
    21961 ttgatgaatt ggttataaat aaagcaaaaa aagaattgaa ggatactgta ggtagttttg
    22021 aatatactcc aattatttga agagtaattt aatacaagta agattgaatt gtatgaatta
    22081 ggatgaatat ataaattaat attattttta acttaacttt ctaattattt tcaatctaag
    22141 atacaataat tagaatatat ttttaaagtt tcctatttaa ggaaaataat ttgattttat
    22201 gtttgataaa aggacttata atttgtaagc ccttttatta tatttatttt ttgtttataa
    22261 ttaattttat tttttaggtt tagttaggtt atagattacc ctgaaaaata acatttaact
    22321 tattattaaa aacatgtaag aatagtttat aaatatttta ttttcgatgt tgtttattat
    22381 atccatttta tcgatggatg aggaatggct ctgccttccc tagttacaga tgtagtaggg
    22441 aaaccaattg cacaggcatc aaatccatat tcacctgttg gtactcctaa aatatcacaa
    22501 aaatctttat tatcgctcaa tcttcctatt acactaacaa gttcaaaacc taatcctaaa
    22561 gcagttgatt taagccacat attctgcatc acatgagata atgacttttg ttctataggt
    22621 ggtattccac gacgttctgc cacaataata agataaggtg ctgttcctat accaagaacc
    22681 cctttttcaa cttgcatttt taaggaagta atgaaggatt gaagggtaat gttgttcttt
    22741 gccttcttct cattgactga tatttgagtt ttaataacat caacaagaat ggtttttatt
    22801 ttattcattt gtaaactgtt atttgaaata acaaagaaat gtctgaaatc tgtttgtttt
    22861 gctactgaaa gtattgcata aggtgcaagt cttccagttt caattatttc ttcaatcagc
    22921 ccttttgaag gtacttcttt tgtaaaactt cttatggatc ttctagttct taatagcata
    22981 tccaaaactt cattaacatt gtttttatct aaattgttca tttttatcct ccactaatag
    23041 tattcttaag atgttctata atgcatccat ataaaggcga acttttcggt catattaaac
    23101 atttccttca aattaagtcg ctatttctat gaacacaatg ttgattaata atctatatgt
    23161 atattataat atttatattg cattatgcaa taaacaataa tatttaaact gttaagtaat
    23221 aaacatttac tataaaattg ttaattaaaa ttaaaaaaat ttatatggaa tgaggaaata
    23281 attcatgaaa aaagtatcta ttgatagaag aactatacgt actaaaaaaa tgattaggtc
    23341 tgcattagca gagttaatag atgagaaagg atttaacaat atatcagtta ctgatttaac
    23401 tcaaagggca gatataaata gaggaacctt ctatctgcat tatgttgata agtatgactt
    23461 gttagagagg attgaaaatg aaataataca ggaaattgaa gaggagacca aacatcttga
    23521 ttcatccaac atgatggaga tagatacctc aagtgaacct cttccattta tggtaaagct
    23581 ttttgaatat tttagaaaaa attcagtaat tattaaatct atcttgggtc ccaaaggtga
    23641 ccctactttt gaaagaaaaa taaaaaaatt tattgaaacc aatttgtttg agaaacaaaa
    23701 actaataaat tttaacatag atgaggcaat aatttcagaa gaatatttta tacaatatat
    23761 actttcagca gacttaggtg taattcaata ttggcttgaa aaaaatatga aagagtcccc
    23821 tgaagaaatg gcactgatac ttgctagaat gtcgttactc ggtccgttaa gagcagtggg
    23881 aattcgtaaa gattcataaa aatagtttta tatatattag actttgaaaa tatatgttta
    23941 aaaaccattt tttactacta ctgtttataa attaggggag atatcttaaa atattagtaa
    24001 aaggagtgct aagtgagtgt aataactatg agataagagg tttagttgat atagtcaaaa
    24061 cttcaaaaga atcaattaga aaagttaaaa tattgcaatt aaatttaaag gagtaatata
    24121 tgaaaaatga aaatataagt tatttggcgt taggattatg ttttggtgtt gttttgggtt
    24181 cattatttaa aaacttagct attggtttat cctttggatt agttattgga gtagtattag
    24241 acagtaagaa aaataaataa aacttcaata gtaacatata acaattatac tatttttgta
    24301 taaaatcatt ggaaatcaac aatagaaaat atttgttatt taaaaactaa aaatacagtt
    24361 taaacgacct aaaaatatat tatataatta ttataacaag ataagtgaga gtaaggttat
    24421 gataatagag taatattttt caattattac tctatttata tttatttagt tatactatag
    24481 tttatattgt tattttattt tcatatgcag tatagggaat ggattacctt gttcatcgaa
    24541 ctcagatttt ttaaagactt taaatcccat atgtttataa aagcctaatg cttgttgatt
    24601 ttgctcattc acatctacat aatttacatt taatacttct attgcatatt ctacaagtct
    24661 ttttccaatt ccatttccta tactataatt acttacaaat aacatttcaa ttttaaaatc
    24721 atgtattccc ataaaagctt ttataatacc tttttttgtc tcttacacat aaaaaattac
    24781 taacatattt agcacattct ttaacttgag gctttattga tattatattt tcttcactta
    24841 aaaagtcatg tgttgctctt actgatgatt cccgtgtatc tagtatttct ttcatatctt
    24901 tattgttaat aatatttaaa ttttcaattt ttatcattta ataattcctc ttaagtaagt
    24961 atgttactta atattatatg ttaaatatga tttaaaaagg ttataagaat atttttgtat
    25021 taaatataat caaataaata tatttatttg tcataaatta aatgtccact aaaatagaaa
    25081 aaataagaag aaataggtaa taaatgatag cttatagcaa aataaagatg taagtataac
    25141 aatagatgag attggaatta agtcactata cctaacagaa cattcttagg cgaatataga
    25201 agacttaaaa aagaaaagag aagtagacaa gaatttatta atgattacat ataaaataaa
    25261 tgaattataa ttttattaat gatataataa taattaacaa aaaataaaag aggaacgagt
    25321 taatgtttga tggcatacac tcattttcta accaataaaa caatagattc aattaaggtt
    25381 actaaagtct aatcaacaca taaacatata ggataaatac atctttttaa tatattaatt
    25441 atgttagact tacaaaaaat gtaaatacat agcttgttaa atcttgattg aaccttaagt
    25501 aataagggat aaaaaatgac ttgttcaaag gcaaagaata tactattaaa tgatataagt
    25561 aacttagaga taatatgtag taagtcaaaa gaactatata gcatcagtaa gtaaaaagcg
    25621 taaaaactta cataatgcac tataaaataa atgagttaga aaataaatat aatttatata
    25681 ataaagtagt acaaaaagga gtatttatgt ttggatatgt taaaataaac aaaatggatt
    25741 taacatttag agaatatgat tattacaaag cttactattg tggactatgt aaatacttaa
    25801 aaagaaatca tggagaaatc tctaggtttt ctttgaatta tgatataaca tttttaattg
    25861 ttttattaac agcagtatat aacccagagt ctataagtac agaggaagtg tgtatagtaa
    25921 atccatttaa aaagaaaaag gtaattacaa atgatattac agaatatgca gcaagtatga
    25981 atattctttt aacgtattat aaattggaag ataatttaat ggatgataaa cgcataaaag
    26041 ataaattggc atattatata tataaaaaca aattaaaatt agcatatgaa aagtatcctg
    26101 aaaaagcaga gtatataaaa caacaactaa atgaattaaa taagttagaa aaagataaaa
    26161 atataaatat agatgaagta tctagtatat ttggaaatat aatgggtgaa gtatttgtct
    26221 ataaaaaaga tgaaaatgag agaaacttaa gaatgatagg ttttaatata ggtaagtata
    26281 tatatctact agatgcctat gaagatttag atgaagattt taagaaagga agatataatc
    26341 catttataga gtatattgat aaaaatgatg agttaaaaga aaaagtaaaa aaaataatag
    26401 taacatcatt aggattttta gctaggggaa ttgataatct aaatctaaat aaaaatgttg
    26461 gaataataga aaatataatt tattctggtg tttatttaag atatattaat atattagaaa
    26521 gtagaggtgg aaagaatgta cagtaatact tataataatg aaaattttat gagagcaaga
    26581 gcattcatag aaaatagtga atttaaaaaa gcatatgatt ttttaaaaac tttgactgac
    26641 aaatgtgcag agtggtatta tttaactggc ttttctgcaa tgaacatagg atattatgaa
    26701 gaaggtgaag acttcttaaa aagagcaaaa tttatggagc ctgaaaatag tgaatatagt
    26761 gatgcactta gaagttatac acaatataga aatgattata gcaatagggc tgacaattat
    26821 aatagaagaa gacgtaatga tttagatgga tgttgttgct gttgctgtga tgattgctgt
    26881 tgttgtctag gtgacgattg ttgtgaaaat tgtgctaagc tttggtgttt agattcatgt
    26941 tgtgaatgtt ttggtggaga tttaataact tgttgttagg atgataaata tgagtataaa
    27001 agttgcttat ggaggagttt tattagcatt aaatgtgata ttgttgactc ttacaaatat
    27061 tattcctgta aatacattgt ttattatggg gttagcatca cttttagtat caattgtaat
    27121 aatggaatgg ggctttaaga gtggaatcgc tttttatata ggttctatag ttttaggatt
    27181 tatagtgatg gcaagtaagt ctcaatggat tgtatattca cttacctttg gaatatatgg
    27241 tattgtaaaa tatcttatag aaaaagatag gtctatatat attgagtatt ttatgaaatt
    27301 agtatttgca aatataatga tacttatatt gtatttttta ttaagaacaa tagtttatat
    27361 tccaataaac atatttatta taggttcatt tgagatagcc tttatagtat atgactatgt
    27421 ttacagttct tttataggtt attacaataa taggttaaga aagatgctat ttaaaaaata
    27481 atagttgatt tttatattta attttaatga aattgtagtt tggataagtg taaatgatat
    27541 atctgaatat acattaaagc ataaagctaa atttaatata ttcttagtgc aacatataaa
    27601 agctttgatt tttagtcaaa gcttttatat gttagatata aatattagat ataatataag
    27661 tttttaaaat agatagtata aattaaatat ccactatatt tacatctgta gatagagaat
    27721 aaataatatc ttttctttct actattatct caacattttt agaaatttcc tcatcaagtg
    27781 atatttgaga taaaagttcc tttgttggat ttattgctat tggatttcca actcttttta
    27841 gcatatttat atcaccatta gtatctccat aggcatacga gtttgataaa tctaaatcat
    27901 actttacaac aaaatcattt atagcattat tcttacttat agaatcccac attggaataa
    27961 ctgtgcctgt aaatatatta ttttcaaaga cataatcact tcctagaaaa tcggttacat
    28021 tatatttttt tgccattttt tcaacaagaa atccagggct accagatata aatataacta
    28081 tgtgtccttg atttagatgc cactcaatcc ttgaacgtgt gtatttataa actctatctg
    28141 cttttaattt aattacttgg tcacttgtaa agttgataca ggttttatct aatcctttta
    28201 gtgaatcaac ataaaggtca catatttcta atagataatc atcataatta ccttgtcttt
    28261 tatcccaatc cataaaagtg tctctagcat gtttatacca atttttttgg tcaattatat
    28321 cgtatttaat taatttttta aaatgttcaa ccataagaga gtctctataa agagtaccat
    28381 ctatatcaaa aaaagctgct atatttttca taagaccacc tcaattaatc atttccttat
    28441 attatattat aaattgtatc taagttgtta aaatatatgt ataataattt atatattaaa
    28501 aaacaagtat tttagagttt aaaggatgtg actatatgaa ttttaatttt gcaaaggcta
    28561 caaattctag acttatgggc agtttaggtc ttataataaa ttggatagat gatgaaaaca
    28621 atcacttttg tcaatatttt ttattagatg cagagggatt aggattagca gattatgtga
    28681 gtttaaataa cccaacacaa gaagaagctt atatggaaga agaaagattg atgggaggat
    28741 ttggttcaga tagagttgaa ttaacgaagg atgaatcttt atttttagta tcttattttg
    28801 gtaataagaa tctttattat gataagctat tgccaggaga taaatgtgag tatattgata
    28861 ttataaaaaa ctataaaaca gatttaatta tagaaaaatt atacaataaa atatgtaaaa
    28921 gagttgatga ggaagttgaa tttataaact atatgactat gagatttata gcatgggata
    28981 gagaaagttt aaaatatttt tctggaagtg acgagattgc aaatatgcat attactaata
    29041 taaatggaac attacttaaa aacgtggtaa gtgataaagg tcaaggcaga tatataagtg
    29101 aagctttata tgaagacaat gatggatatt atatatgtaa aattgctttt tgcataagta
    29161 agtgctatga aactggtttt agaattaatt ctctattagt gacagataaa gaacttatgt
    29221 atgattttga ggtatttgat gagatttcta aaagtgaatt tgtaagtgtt tacagtgtaa
    29281 attcacctga agaatttgca ggagtatttt atagagaaaa tccatttctg ttaaaaagta
    29341 atatgaatga aggtatattt tttactagat ttaattttaa taatgaccat gtaaaagaaa
    29401 atgtatatat aataaacaat gatatgaaag ctatatatta cttaatggga aataagtttt
    29461 ttattggtac ttataacgaa aatgattgca attatatcaa tgaaataagt cagtctaatt
    29521 atagtgacta tattaaattt gaagagaaat ttttctttga gcaaaatgca ttatatgact
    29581 ttgcagaatc aggttcatta gattttgatg attttttgga ataataaatt tagacattat
    29641 ttaagcgaga ttaactgaat ttaatctcgc ttaaatttat ttctataata ttgggggaat
    29701 attatgagga atacatttat tattgtggtc aattattaaa attttataca gtttattata
    29761 aaaaatttat acttttcttt atgttgtttg ccaatattag tctcaaatac gagtaccata
    29821 ctattctgac ccaccaacac aagaaagagt tttttaaatg aaaaagtcag ataaaattaa
    29881 attttatctg actttttcta tgtttatctt attagattat gttaatatct gttgaccgaa
    29941 taagcgcata ctcagtcaac tttctatacc tataataata ttgggggaat attatgagga
    30001 atacatttat tattatggtc aattattaat gttttataca acttgctcaa aaaaatatat
    30061 attacatttc ttcctcatca aattcttcaa tattagtagc ctgtggtata ccttgtatag
    30121 acatattact agaaccataa ggagtaggat atatattagg aggcatagta ctaggcataa
    30181 ctacaggcat accttgcatt gtattatgac atataactcc tggtatcatc ataagtacat
    30241 taggaggcat attttgatta catgagaagt taggaatacc catagtatta ttcatattat
    30301 accccatatt actgttcatg ttattaggca cattgttagg catacccatg ttactattca
    30361 tattattagg catattgtta ggcataccca tgttactatt catattatta ggcatattgt
    30421 taggcatacc catgttacta ttcatgttat taggcatatt gttaggcata cccatattac
    30481 tattcatatt gttagacata ttgttaggca tacccatgtt actattcatg ttattaggca
    30541 tattgttagg catacccata ttactattca tattgttaga catattatta ggcatacccg
    30601 tattattatt catgttatta ggcatattat ttgtagcaga tgtattgtaa ttcatatttc
    30661 cggatgtatt atttgtgtta ggcaagttgt ttggagaatt actactttta ggataatgat
    30721 ttacattatt aggatatagg ttatttatgt tctggttatt aggtattttt gtataaccat
    30781 tagaaatttg agggtcattt ctcaattcat cttggggaat ttcttcatta gtaatataat
    30841 ctgcatagtt cgctgggtct aagtaaggca tgtaactagt atctaaatcc ttttttttct
    30901 tgttactcat attaaacttg cacctcctag aaaactataa aaaagtttac aaaaacttat
    30961 tctaataata tatatattaa aaaagtttat aatatgtgca ttctaattat attaaattaa
    31021 taaaaattat tgttgcaaat ttaggaatga tagcatataa tgaacatatg aacagttatt
    31081 catatattaa aacaatggag gattaaagat gagagaagag ataaatgatt gtaattgtaa
    31141 tatagtacat gaagaaatag taacagaggc taaatcaact atgccagatg aagagatgtt
    31201 atatgattta gcagaattat ttaaagtatt tggggatact actagagtaa agatattata
    31261 tgcattattt gcaaatgaaa tgtgtgtatg tgatatagcg agtttattaa atatgactca
    31321 ctctgcaata tctcatcaac ttagagtact aaagcaggct agattagtaa agtttagaag
    31381 agaaggaaaa acagtgtatt actcattaga tgatagtcat ataagccaga tatttgattg
    31441 tggattaaat catattagag aaacttataa atgataaatg agaggagtga atatatatgg
    31501 aagcaaataa tttaattaaa aaagaattta tactaggagg attaaactgt gcacattgtg
    31561 cagaggagat taataataaa gtttctaaac tacaagaagt taaatcttcc aacttaaatt
    31621 ttataaataa aaaacttata gtaaatataa aggaaagctt taatgaggat actactatag
    31681 aaaagataat agatataata gattcaacag aaccaggtct tgatatacaa ataagttcta
    31741 aagaaaatgc atctagtaaa acatcaatta aaaaagagct tatattagga ggattaaact
    31801 gtgcacattg tgcagaggag attaataaca aggtttctaa attaaaagaa gttgaatctt
    31861 ctaatttaaa ctttgtaaat aaaaaactta cagtaaatat aagtaataac tttaaagagg
    31921 atgatgttat aaataaaata aaagaaataa taaattcaac agagccagga cttgatatac
    31981 aagtagggtc tactgacaaa gtaaaaggga gaactactga aaaatcagga gctgtaaatg
    32041 atacaaataa gaaagaatta gttccactta taattggagc acttgtatat atatttggta
    32101 tatatcaaac agctacaggt tatgaaagtc agtttagcaa tatagtattt atagttgcct
    32161 atgtaatagt aggtggcgat gtgcttttaa gagcaataag aaatatatca aaaggaagag
    32221 tatttgatga gaactttttg atggctctag caacagtagg agcattaaca ataggggagt
    32281 catcagaagc agtaggggtt atgcttttct ataaagtagg agagtattta caaggtgttg
    32341 cagttggtaa atccagaaag tctataactt cacttatgca aataagacca gattatgcaa
    32401 atctaaaatt taattctgaa gttaaggtag tgtcaccaga agaagtaaat gtaggggata
    32461 taatagtagt aaaacctggt gaaaaagtac cattagatgg tgtagtagtg gatggagttt
    32521 ctatgctaga tacttctgca ctcacaggag agtcagtgct gagagaagtt gaaaaaggag
    32581 atgaaatctt atcaggtgtt ataaataaaa atgctttatt gagtattgaa gttacaaaga
    32641 gttttggaga atcaacagtt tctaagattc ttgaccttgt tgaaaattct agcattaaaa
    32701 aatcaaagac agaaaacttt atatctaagt tttcaagata ctatactcca atagtagtaa
    32761 tagctgcttt attaatagca tttgtgccac cacttgtaat ctcaggtgag gtatttagtg
    32821 attggttata tagaggattg atattcttag tcgtttcttg cccttgtgca ttagttttat
    32881 caataccact tagtttcttt agtggaattg ggtttgcatc taaaaatggt atccttatta
    32941 aaggaagtaa ctatttagaa gctttaagaa gtgtagatac agtagtattt gataaaacag
    33001 gaacacttac taaaggtgtg ttcaatgtaa ctaagttaaa tcctgaaggt atatcagaag
    33061 aagaattatt agaatatgca gctattgcgg aggtaaattc aaatcatcca atagcaaagt
    33121 ctatattaag ttactataat aaaaaaattg atttagatac aatagacagt tatgaggaaa
    33181 tagcagctta tggaattaga gtaaagcata atggcaattt tatattagca ggtaatgaga
    33241 aacttatgaa aaaagaaaat atatcttatt catcagctaa agaagtaggc acagtagtct
    33301 atattgctgt tgataaagta tatagaggtt atatagttat atctgatgaa gtaaaagaag
    33361 atagtaaaaa tgccataaga agcttaaaag caataggggt aaaagaagtt gtaatgttaa
    33421 ctggagataa tgagaaagta gcaaaaaata ttgctcaaga attagaattg gatacagttt
    33481 actcaaattt acttcctaat gaaaaggtag atagattaga agatttatat gaaggaagaa
    33541 ctgaaaaaga gaaaatagca tttgtaggtg atggaataaa tgatgctcca gtattagctc
    33601 gtgctgatgt tggtatagct atgggaggct taggttcaga tgctgctatt gaagctgcag
    33661 atgtagtgct tatgacagat gagcctagca agatatctaa agcaattgaa atagcaaaca
    33721 agactaataa gatagtgtgg caaaacataa tatttgctct aggagtaaaa attatagtta
    33781 tgatactagg tgctggagga gtagctacta tgtgggaagc tatatttgct gatgtaggtg
    33841 tggcacttat agcagtagta aatgctatga gagctatgag ataagggata aatgtaaatc
    33901 catactattt aaaaaactta taattatata ttgaagtatg taataatgaa aggtatcaat
    33961 aagtttcttt taactttatt ggtacctttt tattggagcg ctttgcatat ataaatttat
    34021 attcattatc cattgtgttt atttaatgta tttatttagt gtagagcttt tatattgtaa
    34081 ttttaaaatt tagacataga aggtttatta ataagtctat attaagttta aattagattc
    34141 ataaaaatta taatacttat gattgtatat atattatatt aatagttata atttttacaa
    34201 caaaatgtgt gtgttaaaat actctaaacc aacagtatga ctgtcattca ataaattatg
    34261 taatataaga aagtaaatat ttttaaaaaa taatttaaaa aagtattgac gaaatatttt
    34321 taag
//
LOCUS       DAEPOG010000020        33730 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEPOG010000020 DAEPOG010000000
VERSION     DAEPOG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.020"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..316)
                     /locus_tag="KR432_000861"
     CDS             complement(<1..316)
                     /locus_tag="KR432_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0639414.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLH"
     gene            complement(410..1222)
                     /locus_tag="KR432_000862"
     CDS             complement(410..1222)
                     /locus_tag="KR432_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0639415.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(1301..2164)
                     /locus_tag="KR432_000863"
     CDS             complement(1301..2164)
                     /locus_tag="KR432_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBH0639416.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(2425..2682)
                     /locus_tag="KR432_000864"
     CDS             complement(2425..2682)
                     /locus_tag="KR432_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0639417.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(2686..2976)
                     /locus_tag="KR432_000865"
     CDS             complement(2686..2976)
                     /locus_tag="KR432_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639418.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            complement(3007..3573)
                     /locus_tag="KR432_000866"
     CDS             complement(3007..3573)
                     /locus_tag="KR432_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639419.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            complement(3607..3789)
                     /locus_tag="KR432_000867"
     CDS             complement(3607..3789)
                     /locus_tag="KR432_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639420.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            complement(3789..4082)
                     /locus_tag="KR432_000868"
     CDS             complement(3789..4082)
                     /locus_tag="KR432_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639421.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            complement(4097..5623)
                     /locus_tag="KR432_000869"
     CDS             complement(4097..5623)
                     /locus_tag="KR432_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639422.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            complement(5640..6665)
                     /locus_tag="KR432_000870"
     CDS             complement(5640..6665)
                     /locus_tag="KR432_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0639423.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            complement(6677..7294)
                     /locus_tag="KR432_000871"
     CDS             complement(6677..7294)
                     /locus_tag="KR432_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0639424.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(7287..8336)
                     /locus_tag="KR432_000872"
     CDS             complement(7287..8336)
                     /locus_tag="KR432_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0639425.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(8337..8756)
                     /locus_tag="KR432_000873"
     CDS             complement(8337..8756)
                     /locus_tag="KR432_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0639426.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            complement(8761..9021)
                     /locus_tag="KR432_000874"
     CDS             complement(8761..9021)
                     /locus_tag="KR432_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH0639427.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            complement(9035..10927)
                     /locus_tag="KR432_000875"
     CDS             complement(9035..10927)
                     /locus_tag="KR432_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0639428.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(10920..11609)
                     /locus_tag="KR432_000876"
     CDS             complement(10920..11609)
                     /locus_tag="KR432_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0639429.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            complement(11626..13998)
                     /locus_tag="KR432_000877"
     CDS             complement(11626..13998)
                     /locus_tag="KR432_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH0639430.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(14064..14831)
                     /locus_tag="KR432_000878"
     CDS             complement(14064..14831)
                     /locus_tag="KR432_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBH0639431.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            complement(14969..15496)
                     /locus_tag="KR432_000879"
     CDS             complement(14969..15496)
                     /locus_tag="KR432_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBH0639432.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     repeat_region   15619..15908
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=15749..15777
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(16361..17227)
                     /locus_tag="KR432_000880"
     CDS             complement(16361..17227)
                     /locus_tag="KR432_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBH0639433.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            complement(17346..17534)
                     /locus_tag="KR432_000881"
     CDS             complement(17346..17534)
                     /locus_tag="KR432_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639434.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     repeat_region   17773..18129
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17839..17867
                     /rpt_unit_seq="atttacattccatatagttaatctaaaac"
     gene            complement(18871..19032)
                     /locus_tag="KR432_000882"
     CDS             complement(18871..19032)
                     /locus_tag="KR432_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639435.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(19373..19813)
                     /locus_tag="KR432_000883"
     CDS             complement(19373..19813)
                     /locus_tag="KR432_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0639436.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(19885..20712)
                     /locus_tag="KR432_000884"
     CDS             complement(19885..20712)
                     /locus_tag="KR432_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639437.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(20771..21241)
                     /locus_tag="KR432_000885"
     CDS             complement(20771..21241)
                     /locus_tag="KR432_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0639438.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(21258..22568)
                     /locus_tag="KR432_000886"
     CDS             complement(21258..22568)
                     /locus_tag="KR432_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH0639439.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(22569..22745)
                     /locus_tag="KR432_000887"
     CDS             complement(22569..22745)
                     /locus_tag="KR432_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639440.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(22738..23175)
                     /locus_tag="KR432_000888"
     CDS             complement(22738..23175)
                     /locus_tag="KR432_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639441.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(23524..24330)
                     /locus_tag="KR432_000889"
     CDS             complement(23524..24330)
                     /locus_tag="KR432_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639442.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(24440..24730)
                     /locus_tag="KR432_000890"
     CDS             complement(24440..24730)
                     /locus_tag="KR432_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH0639443.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(24730..25077)
                     /locus_tag="KR432_000891"
     CDS             complement(24730..25077)
                     /locus_tag="KR432_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639444.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(25071..25472)
                     /locus_tag="KR432_000892"
     CDS             complement(25071..25472)
                     /locus_tag="KR432_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639445.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(25482..25715)
                     /locus_tag="KR432_000893"
     CDS             complement(25482..25715)
                     /locus_tag="KR432_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639446.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(25734..26624)
                     /locus_tag="KR432_000894"
     CDS             complement(25734..26624)
                     /locus_tag="KR432_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH0639447.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(26636..27199)
                     /locus_tag="KR432_000895"
     CDS             complement(26636..27199)
                     /locus_tag="KR432_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH0639448.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(27267..27515)
                     /locus_tag="KR432_000896"
     CDS             complement(27267..27515)
                     /locus_tag="KR432_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639449.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(27517..29052)
                     /locus_tag="KR432_000897"
     CDS             complement(27517..29052)
                     /locus_tag="KR432_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH0639450.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(29042..30484)
                     /locus_tag="KR432_000898"
     CDS             complement(29042..30484)
                     /locus_tag="KR432_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0639451.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(30490..31743)
                     /locus_tag="KR432_000899"
     CDS             complement(30490..31743)
                     /locus_tag="KR432_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH0639452.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(31721..32413)
                     /locus_tag="KR432_000900"
     CDS             complement(31721..32413)
                     /locus_tag="KR432_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639453.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(32483..32992)
                     /locus_tag="KR432_000901"
     CDS             complement(32483..32992)
                     /locus_tag="KR432_000901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639454.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(33013..>33730)
                     /locus_tag="KR432_000902"
     CDS             complement(33013..>33730)
                     /locus_tag="KR432_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639455.1"
                     /translation="TNIINAHTKEVEGNKKMDQGDSNKQHLFIKFMKENNIAVVLFER
                     VFVGVPMIELSKNLHLYYEDMIENNKFDNNMSKTSINITQIASKDFIEQILDLDGVSK
                     ITLNVDREKFGTDEDNLFSNLNNSRRHNDLIYKPTFRTRYLPSEVKRCCEKYIKGEEF
                     RNKKINRIIIEGEKNKRKLKLDTEGIKLCREIEVSLDIDNHIDSEDIFEKMNNLIDDL
                     IENNSVFFNLMYQEVAMSEA"
BASE COUNT        10110 a         6287 c         3544 g        13789 t
ORIGIN      
        1 tatgtaaatc taactctaag caactctcaa ttttatttaa atcactttca tatattttat
       61 agttatcttt tgatgcaact tcatttgtta atggatcatc cctattatct aatacctttt
      121 caacaatatc tcttctaaag gattcatcaa caaatttatt gcttatatca atttccttta
      181 cattctttct tttataagat tttatgttat tgttactctc taaatctaat gcatttacaa
      241 ataatattga tttgttcatt atcaatgtaa cactaacaac tatcagtaat gattttatat
      301 acttcttaaa tatcataatc tccccccata cctattaata ataaataata tttatctaaa
      361 tattaccata cttatgattt tcaagaaata ctaaaatatt ttatataatc tatctattaa
      421 taaaatctaa tgccttataa agtgtatcaa atctatcatt accctttatc atagtaaatt
      481 tttctttagt catagaacca atcttctcac atgctgcacc accaatgaca taaagatttt
      541 gcgtctgacc tggcacgtaa tcttttatat cacatatcag tattttccca tcattataac
      601 cccaaccaac tacagttgca gagattttgt caacttctcc atcataaaca attgtatgtt
      661 tgtacatctg tttaactccc tcattattta tatttttatt taatacacct tctacaatta
      721 acttagcaat accttcatga cctagtttct tagctttctc ataatcttct ttattatcac
      781 agaagaaact ttcaattaat actgctgtag gatttgaact atttaagata tataatcctt
      841 tatctaattt agctcttcta tttctaaata ctgtacctag cttattacat attctagttg
      901 catactctaa acctttatta ctataatata gaacttctga accttttcct tgaccatcac
      961 ttgcatttag atgtagttct ataagtaaat catatcctcc actattaact ctaggtattt
     1021 tataagtctt ttcttctgct ttagttttaa actgcttttc aggacatatt attacatctg
     1081 ccttatgacc ctcttttcta aatgtatctg ctaatactgg tgcaagagat ttattgtatt
     1141 ggtattcgtt aactactcca tcagcagaag tgcatgcacc actttttaaa atactgtgtc
     1201 ctactgtaat acatattttc attatttatt ttcctccttc ttttcaataa aaaaacactt
     1261 acatatagta agtgtttata aacttcttaa agtaactatt ttatttagcc aatatctcta
     1321 tccaattatt aggaaaacca ataagcttta agtctataga attattataa tcattaatta
     1381 acttttgaag ttttataaaa aaactattcc aaattaattt atccatagtc aaatgcttca
     1441 tagcaagtat ataagaaaat atctttttat tatccaagtt atacttttta tattcgtttt
     1501 ttattgttat aatacggaaa ttattattat atattctacc ataatgagcg cattgatttc
     1561 tgacctgtgt taatgattgt aaccaagatt taactaataa agtattcact ctacacaaat
     1621 tattttttat gtatctagta tcttctggca acatgtttga atataatttt gacaacatac
     1681 caaaagtcat tatttccgtt gcaacccaaa tgggaagttt tccttcatat ttttctttat
     1741 ggtgtataat aaaaagctta tctgaattat tacttttttc tctctctaat gctgtcaaaa
     1801 aattaatata gaatttttca tctttgaaac tctccctttc tagatatcca caagcaccat
     1861 gtttaattgc taatgtatat gcaatgtatg tcctaaatgc tatttctatg ctccctaaca
     1921 aatctgttaa taatatccta aattccttat taaacctata tatatcataa acttcttcaa
     1981 acgtagttcc ttctttatat gagccatcat cattcttaaa acttagaaga tatgctgtaa
     2041 acctataata attgacatta cttaatacaa atttagcatc ttctttatca tttattatta
     2101 atcctctact ttttaaaata tctatttgtt catcaaatgt tttttcttct tttacttcaa
     2161 ccattgtttt caccttttca tagatatact tttatataat aaaacccacc aattaatatt
     2221 agtgggtaaa tttatctgtc ccgcctattt gagctataaa agaagcttgg cgggttcctt
     2281 caaaaatcaa tgtcccgtat atttgagcat ataaatgctt tacgagttct gtactattat
     2341 tatatactta ttagctataa aagtatactc tatttaatga aattttttaa atttttgtga
     2401 gtaataatat ttttgttaat ttcattactt ttcaccatcc ttcaactgtt tgtaagtttg
     2461 atttatacct attgcaactc cccaacaaat tacaccttgt aagactgcaa caggatttaa
     2521 tcctagcatc catattgaga aacctattcc aagtatcagt aacactactg gaatgtattt
     2581 attatctaat tgtttatatt ttttacaacc tttacctata atagagagag cagctactaa
     2641 aattagcaac tgctctggta taaaacttat taaattatcc atctcttatt ttcctccaat
     2701 tctatttaca atttcatcaa ttctaagatg tgcttgtttt gtacttgctt ctatttttgc
     2761 aactcgttca ctaatatcta agaaactagt attaaacttt gctacatcat gctttatatc
     2821 tcttacattc tcacataaaa aagttatttg ttgttctaat tttgtagttg tttctgtttc
     2881 atcttgtatc tttctgtttg agttatttct aaatgctaag taggatatta caactcctgc
     2941 tactgcacat agtagattta tgctaatttc ttccatacat cctcctagtt ttaataatat
     3001 aatttattat gcgaaacaag caatataata acctgtaaaa ggatatgact cataactagt
     3061 atcatatgaa tgaactgaaa aaccagtttt attgctgttt atagtaatcc atgcacgttc
     3121 agaacctcct gtattacttc ctaaagaact accactggga ttataataat atacacttct
     3181 tctaaagtaa acatagacaa taaggtaact tgcactcttg ccaaaattag cagaaaaagg
     3241 attaccatca tatccatcaa taggaccata tctaatagaa tatttgcttt tcaattgagt
     3301 ttctaaattg cttatattgc tcttaaatcc tgtaatatag ttcgcataat cttgaaatgt
     3361 ttgttctgat gtagtaggag agccgatagc agttgataaa agtgtttttc cactatcggc
     3421 ttcttgaaaa gcctcatctg ctctatctat acattctttc aatgctcctt ctacattttc
     3481 acttgtaaat ttactttctg tatcttctat agttacattc tttgcttcta atacaagatt
     3541 tctaacttta ttaaccaact ctttaaaagt catttagtca ccttctttca ataaaaaaag
     3601 aaccctctat atagttggtt ctgctccttc tactactcca ctatttttta taatataatc
     3661 ctctacagct tttctgtact ctgtgttagt cacgtcatct aactgaaatt ctctattttt
     3721 tagaggattt aatcccttac ttaatattct ttctgctaat attcttacta caacattatt
     3781 tatattcatt ataataatcc tcctactttc tcattttctg caattaataa ttgattttct
     3841 aactcttgta ttctcttttc ctcatctgtt atatatactg gtatttcttc taaaatcggt
     3901 tcttttgttt ctatatttat acctataact ctattccttg tataatctat acttccatat
     3961 tcaatatcta tataatgcaa ttccgttatt gtataatgtg gcaatacatc tccacaggat
     4021 tcgcctgttt caaagattgt tctacctgtt tggtcatata ttattcggtt tggaacattc
     4081 atgttttttc ctccttttaa ataaatttaa tagcatacca tttataagta tgtttttgag
     4141 ttgaatcaaa gttataagct ggaatctgaa cgccagtatt attaaaatag acatccccct
     4201 ctgagttact atatagccat ccattagcat gaaaatactc atcaccactt tttctgataa
     4261 aagatgtaac acttgaaaaa tcaacacctc tttgtgtggc aacaccatta caagcgaaaa
     4321 ggaaaagttt ataattattt ttatattcag catcatagta atcaaaatca gcaaagaaaa
     4381 tattaggttt aaagcctaaa ttactaactt taatccaaga accaggttta gtaacattgt
     4441 cggttagata tggataagca attttagtac tatccgcaaa agaagtaact gtaccactag
     4501 caactttata cttagtattt aactgcgata tagtattatt agcttgtgtt aattgattca
     4561 tcaaatcttg taaactagcg tctccactat caaaacctcc cttaattttc tctgataact
     4621 caacgagtgt attatttaaa cttgcatcta tattctttaa tgctaaagta tttataatgc
     4681 ttgttttacc atttctaacg ccttgcccaa tctctgttaa cttagttgat atatcttgta
     4741 atgttgcatc aggttgcaat ggcattatat tcttacttat acttataact ttttcttcta
     4801 caacattttc tgcatcagtt acaactattt ttaatgtgtg tattgcattg tctataagtt
     4861 catagtttat agtcttttct gaatacaagt ctgtagtaaa agtctctttt aatgtttcat
     4921 ctaaataaca ctctattttt gtcaattcac tgtatatttt tgttacagta aacttagctt
     4981 ggctatcagt tactaatgat gttgatatat caattccctt ttctattttt ttaaaagtaa
     5041 aaattctatc aaaattatca tatccatcat ttaattgtat ttttagtttg tgatttccat
     5101 ctacggataa agagtttagt tgactatctg ttactgttgc tgttaaagtt gaattttgta
     5161 caacaggagt aatgttttgt attaatttat catcaagata agcacgtact attaaattaa
     5221 catttggagt atcatcactt acagtaaaat ctacactaaa tccttttctt ttattcccaa
     5281 gtgaagtatc tgaaccagat attacaggtc taccttttat agttattatt ctattagaag
     5341 tatacggaaa tttttgttgt gtatccacat gcattgattc acaattataa gtaaatgtat
     5401 attgaccata ttcccccaag acccaaacat ttcctgaacc ccaatttaat gttgaaccat
     5461 tgtaagtgca acttaagcaa gtttctttaa actcaccatt tagtccatgg tcaccaccag
     5521 tccactcaaa attttgcctt ccacctgcaa atggattaaa tgcaagattt ttagtatatt
     5581 ttcttttgtc aggtaaatta aaataactca ttgataacgc cattttttca cgctcctttc
     5641 tatataggta gtatgtcgtt atttacagtc gatataatat tactattaat tcctgtcagt
     5701 tgctctgcta ccttaccaat ttcttcatgt atcttttttg atgaataagt agtcatttca
     5761 gacactctat tatcatctac agtcgcatta ataaaatgag tctctgcatt cccattaatc
     5821 acataaacat ttaactcaac ttttactcca cttctaacct caatagaatt atcgtctata
     5881 attgtgaaat cgggtactac attatctttt gtagtagcat ctataatact tacagttatt
     5941 ctctgtgtta ataaaccatg tgttacagtt gctttgaatc caccttcttg attatctatc
     6001 caatcatcaa ttgttattgt ttgagtagat gcaacatttg aaccacctgc tattagctgg
     6061 tcaattttaa tattttgttt ctcattttct gtgtcaattc tagtgtttaa ctctgtctta
     6121 gcagtttcta tgttgcttgt taactctgtt ttagttgtat caattttagt gtttacattg
     6181 cttattttag catctaaatc ctgtatatct ttgagcgtag caaaaattat tgtagggtca
     6241 attttaagtt ctatattatt tatattagat acaataagca cagttttaac cttcatatca
     6301 acaactgctc cttgttctat agaaggttta taacattctt tatatttaga gattgcaatt
     6361 agattatttt catcatctaa atatcctatt tctcttatca taaatccacc tacacttgat
     6421 ggtattaaac tctctaatat tatgcagttt ggcgcagttt catctgtggt tgtatttccg
     6481 atgttgcctt cccataccac atttttaagt tctgtctgac tttcagtagg agtatactca
     6541 cttcctccac catcaccaag ttgaattttt acaaacccta ctttattacc tgtgacactt
     6601 gcatttgcta tctttgcttt tcctacatct gtaattatag tgtaataact tttatctata
     6661 gccaatatat cacctcctaa aatattgtta tctcttggta tccaactcca ttgccagtta
     6721 atacatcaat ttctccataa gtttctatat ctggtggact ccaagggtat atagttattt
     6781 cttgacccat tagggttgtt ataccaaaat tcatataatt gtctttgctt ataagcactc
     6841 tagtgtaatc taaagtcata ttacatggct taatattact tacaaaagaa tgaacttcct
     6901 caaaccaatc ttgatttcta gcatcactct caaggtgtat gttataagta gcattattaa
     6961 tagttaattc ataattgcct tctccaacta cattatctag ccagttcctt aaaaatctct
     7021 ctgagtaagg taatttactt atatatttac taaaaatcct aaaccttcta tcttctaaac
     7081 tctcattact tttaggagtt atagacatta tcttttccca tctttttata ccacttatag
     7141 ttaggtcctc taaaaactgg tcatttgata ggtcctttaa tttatcatgt agtgttttta
     7201 tttctttatt ttctacatta aatactttta tatattcttc tttatcttgt agaatttgtg
     7261 gcaagtaatt tattagatta atctctttat ccaactacct cacctctcac tactatgctg
     7321 ttactatcta ttgttagatt agatttaaca tcatttatca ttgtatttgc aatgtctaat
     7381 actccatcaa tactaagtaa tctagtttca atttgagata tacggactat taagttttct
     7441 tcatcttccc aactcatgtt aagttcattt aaatagtcgt ctattgcttc ttctgcaatt
     7501 gattttatat tctcccaagt gtagccattt ttgtatgtta tttctgctga tatgtttata
     7561 gttgtacttg taactccttc aactgtgact cggtgtccaa tcggtgctaa tcctaagcct
     7621 tctccttggt gtccaattgg gtcaatttct tcttgaacta agtttactaa ttcttcactt
     7681 ggcactttaa aattagagtt aattattact aacttaacag tcccacctcc atcccaagtt
     7741 ggataaactt taactcctcc aacatcttgt attttgttaa cttcatccct atagttttgt
     7801 atattcccgc caaaactctg tgaatttagg ctatcataat atctttgtct taaactatct
     7861 tctgactctt catcctctcc atttatcaag atttcagtta gttcagcagt ttcaagacca
     7921 tctatatatt caataggtat tagttttcct aactcaaata caggtccagt agtttcacat
     7981 ttcattttat atgttttttc agatattctc tcaattgcta catagttata ttctcctaga
     8041 ttaaacctag aatcaagtgg aatatctatg ttaaaaactc ctttagcaat cgtatgagtg
     8101 gcttcaagtg gtgttattcc cctttcttta catctcttct ctaaataata ataactagca
     8161 gtatctacga atgtttggtc tagtaattca tccatagcaa tgtatgtttc tgtaagttct
     8221 atagcaacag gagcaagagc attatatatt atagaacctt cccttttatc aaaagtatct
     8281 ggtacactat ctaacattct tttaattata ttttcaaatg tcattaactc aaacaattat
     8341 acactcacca ccttctctgc ttttatattt ccatattttg tatgaactga aaatctacat
     8401 tgtactttac cctttatatt ttgaaactca aaattatcta tattttcaat cctatcatct
     8461 tgaattagtg cttctgttat ccttctttca agttcgggta ttacatatga aataggttct
     8521 ccaattaaat catttaattc aacaccataa tttctagaat aaataagatg ctcatacctt
     8581 tcagtattta aaattaaaaa aatggtttgt tttaatgctt ctacatcatc acaaatacca
     8641 tcaatcttag acttttctat atgaagttta aatgtcttac ttggctcttg tcttacatca
     8701 aaattaatta ttgatacatc ttcaatatca taatctaaat tatcacttgg taacacttta
     8761 tcacatccta tctaaaatca agtattgttg ccctccttgc atacgaatta agactaattt
     8821 atctcccatt tttttatctg tatatctttt aaatgtatct gtttgtatta gaaaaatttc
     8881 accaatagat agtttttgtt ctatcttaac tcttagagga ctaatacttt ctattgttcc
     8941 aaatacaacc ctcattgggt tgcttgtttc tactgcatcc attgcagctt tttttattat
     9001 ctgtaataaa tcttggctca tattgccacc tcacttatat aaatcttctc acatgtgtgt
     9061 atgcttttcc ttttctataa gaattaacag actctatttt taccacatct cctgtttgtg
     9121 gtgaatgaat tatttgattg tttccaatgt acattacaac atgattacta cttcctccac
     9181 caattctaca taataagtct cctgctttcc acttgcttct atcttttaaa tctactgctt
     9241 ttcctgcttt actttgtgca gaagcagtac gaggaatttt tatacctatt tgtttataac
     9301 accattgagt aaacccactg caatcaaaat tattaggacc ttctgctcca tacacataat
     9361 tacaacccag tttacttttt gctatactaa ttaatttatc ttctttagaa ttgttatttg
     9421 tattactttg gttattacct tcaacttgat atgtttgttc ttcatctcca cctataatta
     9481 tatagccatt ctttctacca aattttttac attcactagc attagctaat agtatatcta
     9541 tatgatatgt tccgtttgtt tcaacatata ttcttcctcc attatcttta actgtatata
     9601 ctttgttgtc ataggcagta ccaggaagta taatttttac tttatctcca tattcaaaaa
     9661 ctggatgttt atttagaaaa tcatcagtat accaagtttt cttaactcct tctcgattca
     9721 ttggaccagc aacagttctt gattttacat caagtggctt tccattgcaa tctgtttttc
     9781 caccttccat tgcattgttt cctggataat atgcagtaaa tatagcagga acttttttac
     9841 ctgtattttt tttcataata ctttgtgcag gaccattttt cttttcatct ttattattag
     9901 tatttccact actatatgag cttgaagaat atgaagcaaa ttcgtctcca tcaaccaagg
     9961 tcaaatccat gaaatgtgaa ttattttcaa atgtatgttt tactttctca actaacatat
    10021 aattttgcaa ttcaatatct cctagattta aaaaaacagg tactaaacaa cctgctctca
    10081 ctctaatatc tccaagtgca ttttttaaac ttaatgactt agttttctta ttatatagtt
    10141 ttagaagtat atcacacttt tgttttatct ctgcttcact catgtttttg tctactgtat
    10201 caaacatttg aagtattccc caacttctca tgtgagctga gtcttgagca atatatacat
    10261 ccctttttcc tgactcctcg ttgtctctta caagtttaat ctttgtgtaa gtatcactat
    10321 ctattgatga attatagtca aaatcctcaa ttacatcatt gttcatgaca gtatctagtt
    10381 tcattgatgc aacattcttt aatgttattc ttccaaaatc atcatacaag gtatacattt
    10441 cttttttctc tcttaaagta tcatctagtg ctgttagtat catgtcaaag agtgttttat
    10501 tttcttcgac tctagatatt ttatacttag tatcttctat gacattgtat tttaaattaa
    10561 aatctttagc caagattttt acaagttcac ttgcagtttt attactatat acataagtat
    10621 ctttattctt aaaatatctc aactggtcgt aagcaacaat tttaatgtga ttttctttat
    10681 ctcttttctt ttgaaatata tatccataaa atatgcctat tcctttataa tacagcctta
    10741 cagaatttcc ttcgcaaaac tctagtatat catccatgac tattgtaaac tctaacttag
    10801 aaggtgtacc tcgtctttct atctcccatg tgataccatc aatgacagca ggttcgtaga
    10861 aatcttccca atgagctatt actaatctta catctctatc atttgctaac actaattcat
    10921 caaccaagtt ttaacacctg ccctttataa atagtgtatt tacttaagtt tttgccctta
    10981 tttgccttat ccatcataga tttatttagt tcgtatactt tcttatataa tgaaccatta
    11041 ccaagttgtt tctgacaaat tgaccaaagg ctatcccctg cttttactgt atatgtttta
    11101 gtgtttgtgg cattgactga atcaactcgt tttggctcta tctttacatt aggtctacca
    11161 gtctcatttt taggaggggc aagaactaac tttttagttg agtaatctct atattgcttt
    11221 aattttattg caacttttgt atctgagcca ttatctgcat cttctgaaat agcatactct
    11281 tcaagagata cttttatatt agtgttaaat aagaccttgt tacctaactc acgagataca
    11341 ataaattgaa atggcttaca atcagttttt agtaattcta gcttacttaa aaagaattga
    11401 acatctctaa attgacctct acaaaaaggt agtttattat gtgtaaattc tgcttcaaaa
    11461 cttatttcag ataatccttc tttttttaat atgtttactt ctccagtatt tatcaaatca
    11521 actgtcttgt tcttgtttgt cactttgact tcaagcttgg gtggtgcgat tggtagttgt
    11581 actccatcta ggtaaaaatc ataagccatt ttctcactct ccttcctaaa ctattccttc
    11641 cgctgataca accatagcat cgttcagttt ttcagttaag acattaacta ttccatcaac
    11701 atctgtatct ttacttatgt tgtttgtatt gttcatatct attttaatgt ttactcctgt
    11761 gaatcgattg attgtttctt gctctgctat atctcttaag tattttaagt cctcttgact
    11821 tttatccata gtctttgcca tctttgcagt attgcctgcc gtgtccttta cattttttcc
    11881 taaatctcca ccaaatccac caaggttgtt taaaggattt tgtttattcc acaaatcgtc
    11941 taatcctaat tttttctttg catcttctag catcttatta atgtcaaaag tatctttaaa
    12001 tttatcagtt atagcatttt gccatttagt tccaagtgca tttccttttt gaaatgctgc
    12061 tccaatatct ttgtatccca ttcgctccaa ttttactttc tctggtgcgt ctcctaccca
    12121 tctgtttaag ctatcaatct gttgtttgat gtaactatta tctgccttaa ctggcgtaaa
    12181 cgttgcctct cctactttac caatatttat ccctggtatt ttatttatta ggtcaattaa
    12241 tttgtttaca cctcgtattg cgatatttgc cccatctatg aaagctttcc caagtgcatt
    12301 tccagcatta tttacagaat tatttagaga tgccattttc tcgattataa agattacacc
    12361 ttttgcaata gcttgtttca ttagatatac acattgattc caaccatttg taatgccttc
    12421 atttacagct atacagccat ttaataacca aatcataaca ttttgtattg ctgccactgc
    12481 tgcaaataca gcacccacaa ttactcctaa ggcagttaaa cttgtacctg cgaatttgtt
    12541 tactgctgct actgcaacaa aaactactac tacaaatgct agaataccta atactaccca
    12601 tgtgattgga caagctaata aagctgcatt taaaccccaa gtggctattg ttgctgataa
    12661 agtagctcca ttcgaacgca tcagtgctgc tgcttgtata ttagtcataa gtgctgatag
    12721 tgaagaggaa gcagcgtgag caacattcgc aattacgtta cctattgtag ctattttagt
    12781 cgctagcaaa acccctctaa acaatataaa aacagaagct acaccaaata caataggttt
    12841 tattatactc caattttgtg caaatatatt aactatattt aatgcagtat ttacaataga
    12901 acctaagctt ttagttatta ctaccgcaca aaatgtaaat ccttgcacta agtttgaaaa
    12961 gaattcacta tttaaaatat tttttataat atttaatgta tcgtaaatgt ttgaagatat
    13021 tgcaatcata acatttctta tagatacaac aatttcacta aatctaacgc tttgcaatgc
    13081 tccacttatc tttccgaggg tttgcccaaa aatcatgtag gcgtcatttt tcatcatagt
    13141 aagtgcttga ctaaaagtga ctggcatact tgcgaatttc ttttctatct catcagaagc
    13201 tttaaatagt gcatttctta taacatctgc tgttattgct ccactacttg acaattcctt
    13261 taattggtct ttagtttttc ccattgcatc agctatttta gtggctaata aaggtgcatt
    13321 ttccattata gagcgaaatt catcaccttg aagtttccct gcacccatag cttgagtaag
    13381 ctgatacata gccgcactag cttcatttgc tgatgttcct ccaattacaa aagctttatt
    13441 cataagttct gtaaacttta tcacctctgc cgaacttcca aaagcatctc ctgctaatat
    13501 tccaagttta gcgacttgtg ctgccgtatc tgcataacta gctcttgcac tttgagcaga
    13561 taaataaatc atcttattaa gttgttctgt tgtctgtaat ccatcattca ttaaatttaa
    13621 acgtgctttt gtacttgcaa ttgtatctgc tgcttttgta atactttcta tacctcttat
    13681 tccaatatat atgccagcca aacttttgag tttagatact aatagagaac ctgcttcact
    13741 tccttgcttt atcttattat taaagttttc ttgttcattt gtatttcttc caattttctg
    13801 ttctatcctg gttaatatgt tttcaatatt gtttaaactt tgttgagatg cttgtatccc
    13861 acctgtgtta agcggattat gcaatctttg ttgtaacctt tcaaggctat taattgtcgt
    13921 attaatagat gtagtcatat tcctaaaagc aggtgtcatt ccgtcgaaaa tctttattga
    13981 agtttgtatc gttgccactt tctcactctc ctttcataaa tttccatata aaaaaacacc
    14041 tacattagta agtgtttatt atattacaaa ttcaataatt cttttttctt agtattaaat
    14101 tcatcttgtg taatagcttc catatccaaa agattcttat attttaatat ctcatctgct
    14161 acagatttag tatttatatt gtctgtctct ttaatgcttt gtgtaatagc agataatgtt
    14221 gataaaattt tatgggcatc tttgtaagct ttctcataag cactagaatt agtttttgtt
    14281 tttttattaa ttaattcgat atactcaata ggattatcaa tattattaat tgttatttta
    14341 attttaaatg tatcaacaac ttttcttgtt gtttttttac ctgttatccc tccaactata
    14401 gctcctgctt ctccaaataa aattcctccc gctaaagctc ttcctattcc tcctttagtt
    14461 acagtttcac cattttcaag aagttcaaat ttcacgatat cttcataaga atgaatgata
    14521 ggagtttttt tatcgtttat acttttttta gacacaagcc attttttatt atcttcatca
    14581 aattctatat agttttctat attattagtt gtattaaata ttttttgaag ttcattattt
    14641 tttttagttg atgatatagt cttttcaata tctaattttc ttaaaatttt aggtgcttta
    14701 gtcatagaaa gtgcatcaca atattcttta aaacaagaat tgcatatcca accatcttca
    14761 atattcagta ctcctatctc agtattacaa atacaacata tatctgcttt tttctttcca
    14821 aataacccca taagattccc cccagtacaa taattttata atgtaattat actatataag
    14881 taaaattttt acattattat cacctccttt taattaaaaa agcacttact tatttaaagt
    14941 aaatgcttca tatttttaaa tctatatatt atttttcttt taatataaat tcttgctctg
    15001 aaccactata ttttagttta caatcagaaa ctagtaaatc cttaggaatt tcaaatgcaa
    15061 tattacctgt tacatctaaa ttaggattta cagtatccat agttatatat ttatttccta
    15121 catcaatcaa taaagatgga acatattttg aatcatctgg tcctattaat gtgaatgaat
    15181 ttagcataaa agattggctt tccttagttt gattttttat tgttaaattt ataataccat
    15241 atttgccact atctggagtg tatgctaaat aaccagatgc tactgatatt tcactagctt
    15301 ttgaaatgct attaacagtt aattttaaat ctcctatact accttcttca ccaatatatt
    15361 tagttaaatc ctctttctgt ttttcagtgc tttcattatt agtactgttc cctgctattg
    15421 ctccaacaac tccagaaaat aaaataataa gaataaacca aaatagacat cctcttcttc
    15481 tctttccttc tctcataata tccccctaaa ttatatttta taagaatatt atactatatc
    15541 ttcaaaattt ttacattata atcacttcct ttcaataaaa aaacacctac attagtaagt
    15601 gttttttaat tatttttaat tttaagtcca catagttaat ataaaacctt ctgatttagc
    15661 aaataaagct gtaaataagt cttttacata ccacatagtt aatataaaac actgatgtta
    15721 cagattcgtt gtatgtcttg ggagctgtat ttacattcca tatagttaat ataaaacgtg
    15781 atgctatgtg tataaagaca actactaatg ttgatttaca ttccatatag ttaatataaa
    15841 acttgatgtt tcaatcttaa atacactacc attacaaaga tttacattcc atatagttaa
    15901 tataaaaccc caaaataaat tgtgtatttc caagacttac acacatataa ctttctcaaa
    15961 tttgcagtga gcgggtaata gtgcaattga taacacttat caaaccttct caatgccttg
    16021 tattccaatt gttaaactat acttgagtag aaaaatcgaa cactgcaaaa ttcctatatt
    16081 ttattatacc attttttacc atataaagca cttgaaacag tgaaatatcc aagtgcttta
    16141 tctatttatt taattatttt tctctaattt tttctaagaa agtttatata ctatactttc
    16201 tatcttatta tatatatcta tatctgtttc tcttattttc aaaagtttaa ttctaaattc
    16261 ttcttttctc ttttcaatat ctatactatc aagtaaattc tcatgcattg tattaatccc
    16321 cccaaaacta aactaaatta tttaatacaa tcttataaat ttactcaatc ttataaacca
    16381 catgataatt cttcttttca cctgcaatct tagtaggtct attattttcc tctatccaat
    16441 ttctaacctt atctattaca ctctttgtat atttatttac agtaccagtc caagaaccat
    16501 tagtttccca aacgcctttg acttcgtttt cttctaaatc aatcttttta ataatttcac
    16561 aaacagccaa ctgtgctggt ttattactct tagaatatat tttcagttta gatgctattt
    16621 gctttgtatc aaaataatgt tcttcttcgt ttatctctat tggtaaatca attcctgctt
    16681 tcttatataa tgttttagct gttaatagtt tggatttatt gtcaaagcct gcaccatcta
    16741 atagttcttt taacatagat gtactattgt aagctaactg taatttctca atctcacttg
    16801 ctttttctct tagtttttcg ggattagcat tgttagttat gtatgcacca gtttgtctta
    16861 tgcttggtaa aacttctcgt ctaagccatt tcctaaactg tacaccaata ggtttgtctg
    16921 tgtattgtaa aaatccatat aaaccatctt cataaaaaat agttatactt ctagctttat
    16981 tactaattat attatttgcg actacattta aagtagttac aaaatcatta aattcatttc
    17041 ctttcaatac atcatactct tgttcaatct caaagtcttc tgcttttatg caatcctgta
    17101 ttgttttaga tacatcagca taatcgaata atccaactat ttgattagct atccaacaag
    17161 atttttcttt ccacataaaa gtataaattt gacttccatt gaactctttt actattaaat
    17221 ttttcataac tattacactc cttaattgaa ttttttcaag gaatgacgta tactatagtt
    17281 agtgtatata atatacgtca ataagggatg ttcaatcttt ggtcggggag aacgtctctt
    17341 attttttatt cctctttttc tagctcttca ttaatctttt cttcaagcca aatagtttta
    17401 gtcttgttct gcttttttaa atgttcttca attttctcta ctttctctct atctagtaga
    17461 acactaaaag tttttttatt ctgtcgtctc tgcttgaagt aatctgctct actgctatca
    17521 gtaataattt tcacctcttt tctgtatcgc gataatataa ttatacattg tatcgcgaca
    17581 cttttcaaga gttttttaac atttttttct aattatttta ctcaaccgac caacttgagc
    17641 aaaatcaatc actttagtgc atttttatat tgtataattc taaaaagttg tacttagcaa
    17701 ctttataatt aagtaatttg agcaaaacaa aagcacctac tatttaagta agtgctttct
    17761 tttttctatt taattttgaa tccacatagt taatataaaa cattcaagaa gtaactgtaa
    17821 ttgttcatct gtaaaataat ttacattcca tatagttaat ctaaaacgat agacaaagct
    17881 agtgcagacg ctaaagtaca atcatttaca ttccatatag ttaatctaaa acctttcata
    17941 actgatgtta aagactcatt atatgtacta tttacattcc atatagttaa tctaaaacga
    18001 gtgttacatc gacaataaag cttattctca ctgaaattta cattccatat agttaatcta
    18061 aaacagaaag attaaaaaat agaatgatac ttcttacaac atttacattc catatagtta
    18121 atctaaaacc ccaaaataag ttgagtattt tcaataccta cacatataca actctcttaa
    18181 atttgcagtg aaccatgagt agtgcaattg ataacattta tcacacaccc tcaacgcctt
    18241 atattgcaac tgttaaactt caatttatca caaatatcgc tcactgcaaa atctctacat
    18301 ttttattata tcataaaaat attatttttg aatatctgta ccaatttgtg gtataataaa
    18361 agcaaggaaa taatttattc ctacaaagag tgattatttt cagttgttaa gtgaaaatat
    18421 tattttttta aatcaccctt attggcgtct gggtgatttt ttattttgtc ataaatataa
    18481 gctgatataa caccagctag tatgcttaat aaaaaaccta tcatataatt tcacctcctt
    18541 ccttatttgg aatttggcgt ttaatatgaa aataatcacc cttcgcactt tcgattatta
    18601 tccttgctac aattattata acctataatt attacatatt tttccatttt ttttatataa
    18661 acaatgaaat tcaagtaaat aaatacctac ttatttatat atattttata aattaattgc
    18721 tttataatca agttttcatt tttttaataa aaatttttat tttttatttt atataaacaa
    18781 tattttttct aatttgtggt ataataaaag caagaagaac tacaatctat ttgacagtag
    18841 agtgaagttc ataatttaaa caattagtaa ttatttaaat ttgtggaact tgattttaaa
    18901 atcaaattcc cagccacttt tactcttgcc acgagttgag tggcttttta cttttagaaa
    18961 tactttacaa attaagcaaa atattaaact agcaataacg ccagctatta cattaagtaa
    19021 aaagttattc atacttccca cctcctttca ttaggaagta ggttttatcc cagtatgaac
    19081 tccactctat aaattgtaga ttacatcttc ttgctaaaaa tattataaca tataattatt
    19141 acatatttta cctattctat atttattttt ttattttgct atcttcttcg tcccctcttt
    19201 ctctctcttt cagcttcttt cattgcttcc tcttcatctt ctatctttat aagtattgag
    19261 gcggctgcta acgctctctc attaacttct aaattcatat attcacttgg cttccacttt
    19321 aatttttgaa tacaataatg agtgatgcta gcatcaaaat cgccacctct gattagtttt
    19381 ttgcttcttc tactttatcc tcaaaagatg tatcaaatcc attgacttca ttcactttta
    19441 ctgtataatt gacatactca cctgctgtaa gcattgtctt taataactga gcttctccca
    19501 ttactccata actattttgg agttcggcat cctttaaatc tggaaatact gtagatgcta
    19561 cacataattc agctacataa ctattgtagt caatttcact tgtatattgt ccagtatgct
    19621 taccattgtt accaatcact tttactcttt tagtacactt tcttcttagt gcttcgtctt
    19681 cttcagatga taaaactttt aattcccatt caactggttt cccttcttta tctaaaaatc
    19741 tgttactcgc tacatatttt acattatcaa cctttattgc attttgactt aaaaaagcac
    19801 ttaaattact catattattc taatctcctt ttattttaat ttttcatata aaaaatacac
    19861 atttataact tataaatgtg tattttactc catacctata ttttttatta cttcttccaa
    19921 tggaattttt ttagtaacta attgattata aagaaataga tacttaacat tcaaattttc
    19981 tgcacattgt tttaaagtaa tcatttcacc tttataatta actctaatac tatttctttt
    20041 attatttcgt tgaacttccc aagtaaccca tctacaattt gatggttcgt aatttccatt
    20101 cacattaatt ctatctattg ttaaaccttt tctatatcca ttttctatag accagttata
    20161 aaaatttata aaatcctcta gccattcatt gcatacttct atgcctctac ctccataata
    20221 attataatct ttattactct tatttgtgca ccttttaatc atacggtgat aaactctata
    20281 taagctatca ttagatttat tatgcgttgt agagcgctct agtaatattt cctttcttat
    20341 acagccacaa ctttgtgctt ttccttgttc taatgcactt gtactagtta ttgttctatt
    20401 tccacattca cattgacata accataaatt gtgactatat ttatctttcc cagacatttt
    20461 caatactgta agcttcccaa aagttttccc agttatatct ttaaatctct tattttgtcc
    20521 tttccagttt ggagaacaac cacaatgtgg aggcttctca tttttaattg attgctttaa
    20581 attattttct actctctcaa ctttattccc acactcacat tgacatagcc agactttttt
    20641 actatgttta tttctcccta aatatttaat taaagtaagt ttgcctaatt taattccact
    20701 ttcaaattcc atgaaatacc tccaaataat ttatacttac atcataatat ttttcaagta
    20761 taattcaatt ttataacatt ccattaagta atgcaaagga ttcaactaat tcccaatcct
    20821 caaaagtgaa atccatatct tcatctaaat actcaccatc agcatcaaat ttagtaatta
    20881 ttccactgtc catattacaa tctttaagta ctactgtctg tcttcctaca gcagatgtag
    20941 ggtcttcatt tgtaacttgt atgtcaaaat aaatatcctc accagtttct ttatatctgt
    21001 aaagtaattc tctaaaaata gaagtattat aatgaaatgt tgcacttcca gtatttgtac
    21061 tcccagttgt tttatttccc tttgttgttc ttcctagaat tggaacttca cttttatttt
    21121 tttccatttt agcctctaaa tctatagctt gcataaaatt atatctttta ccttctatag
    21181 ttataaaaca ttcagctttc tttgcactaa ctgtatcttt agcatttatt gtttgagcca
    21241 tattatcact ctcctctcta attaactgaa acagtcatat aaagcttact catagcattt
    21301 attaccttaa tagcatcaga tactatgaca gttttcttat catttccaag ctctacacta
    21361 acatcatcag ttttaaaatc ttctattgcc cttatattct ctaattcttt atggtgttta
    21421 acaacatcat tccagaaact tattcttcct gccttatcat tcggaacttt acctaaatac
    21481 ttttcattaa ataaagttgc aatatcatta gcaatttggt caagtactct aacactttgg
    21541 ttacttgaaa aatcgtcatt tttatcatct gtaaatgata caaaagtatt tatatcctct
    21601 aacacatgaa cttcatctcc caccttgtgg aatataaatt taccagtttt taaagcttct
    21661 tcaagttgta tttgtgtata attaacatca acatcaaact caccatcata ctttttatta
    21721 gtattagatt tatttatatc gcatcctgct atagctccag tagcccaata aatcaagcta
    21781 gattcaacta aatctttatc tttaatctta ttttctatag acactacacc ttcataatct
    21841 gcatcacttt tcttatatag tactgtttga aacttagctc ctaccttatc tctcattctc
    21901 tttgtaaatt ctacaaataa acttttaatt tctgttgttg tagccaaaca ccctagtgca
    21961 ttaaatgaat aactttctat tttatccaag aaagcttggt actctgctcc tgtcacagct
    22021 tcgccattgg ttccaccagt aaatacaagt cctgcacttg cttctagtgt tgcatccttc
    22081 ttccaagtga tatagtcatt gtcttgtaag tctgtaataa cctttgctat ttgagtatct
    22141 accttcttat tatctaaaag tattacaaca tcaaacttag cattatcatc tatatttgtt
    22201 gtaactgtta cttttaagtc atttcctctt ataccactat attttgctgt ggctatagta
    22261 caactggctt taacgccttt atttaattta taaaaatatc ccaaccttat atttttgaat
    22321 aaatctctca aacctttcag cttctcatga gtataatcat atccaaaata cttcactgaa
    22381 tacttctcaa aatcatcact ggttacttgg aatgcttctt catctatgcc ccaatctaac
    22441 tctaaaggca ttgcaacaat acctctatct gataatgaac tggttgccct cttagctgag
    22501 ataaaattta tatatgcacc aggaagtatt ttattttgcg ttacgaatgt tcctccacct
    22561 aaagccatct aactcactcc tttcataaat ttatttattc tatcctctac ctctgagaag
    22621 gaatataact cattttcttt taaaattgca tttaataaat cttttctatt tacatacttc
    22681 ttagagttaa ctatctgctc cttagtaaac ttgtagttat cttctttgct taatgcttta
    22741 ctcaaaatta tcacctctct tcaaaccacc gaataactct actgtatcca tcttattggt
    22801 atcattattt tttatagtaa aatagttata atcaacaaag aagtgaagaa cattgtctat
    22861 aatttcaaaa ttcatatttg tacctcttac taaatctcca tcaatttcta tatactctaa
    22921 ttcctccagt agcatctcag ctatctcatt tatttcaaaa ttcttatctt ctgaacgagg
    22981 gaaataatgt acatcaaaag aatttttctt taattctctc ccgcttggat atggtgtctt
    23041 gcttggattt aaaggaacaa taaaaaaaca aggttcatta ataccttgct ccacatcttc
    23101 gctataaatt gtatattttt ctccaaacga tttatctaac tttactgata ttccatctat
    23161 aatattatta agcatcaaac actcctttaa gcaatattaa tagttttttc tctattatct
    23221 tatcaacttg actttgtagt tccatttctg aaatagttaa gaaatgttgt cctttaaccc
    23281 aacccttgcc atttttagtt ctatgaccaa attcaacata tgcattttgt tatcctaaag
    23341 gctttttatc ctctagctct tatagtttcc tataagttcg gcgtacatca tcaacaaaat
    23401 aaactttatt tagttgccca gcactcttgg agagattata tttattcact ctctacgctc
    23461 tacggaaacc tatagcctat ccgcaatcta taggttttcc tcggtattgg catatataat
    23521 taattaaata tttttccatg ttcttctatg gactatattt gatatagatg aatatgttac
    23581 aggatatatt tcacttagtt gttttattgt atatccatca gaatactttt ttctaatttc
    23641 ttttacatcc tcaatagata acttagctct gctttcttca ataatcttaa ctttttctat
    23701 aagcttattt cctactaatc ccttagaata tctacttctt aagcaagagt atgaaatacc
    23761 tgttttttca gaaagctcta tcaatgtaat ttctttttct tcatgtttta ctaatatatt
    23821 tgttgaccta tttctacatt gtgtctttat atccacccat ctacaattgc ttgattcata
    23881 atttccttca ttatttattc tatcaattgt aagtttctct gaatatccat tactcaatgc
    23941 ccaatttgca aaattatcat agcaaaacca ttcatcacac acaattatat tcctttcacc
    24001 atatctattg tatcttttat tatttttatc tgtacatcta gatattatac ccttccatat
    24061 actgtaaagt cttttatttt gaaccttata tttcttttta aattggtatt tgtctgttaa
    24121 atttaattta tcttgttctt tttttaaaca tccacaagat tgcactaagc cactagttaa
    24181 agagtcagtt cttatttctt tgaaatttcc acaatcacac ttacataacc aatatttcct
    24241 atttctttta ccgcttttta tctctttaga aaatttaatg actcttagcc ttccaaattt
    24301 ttttcctgta atatctaaaa attttgccat aaaatcacct cttttataca ttataaatca
    24361 agagttattc tatgtccata ttctacctac tttataatgt aacactatta cacttagcgt
    24421 ttaccgattt tgctgggttt tatatgcccc attgtgttaa ggcatattca gttggattaa
    24481 caacttctat aatataatta ttaccttgtt tgtaaacagg aagcgaccta gcataagcta
    24541 ctccactcca accctctctc aaaaatcctg tatcaactgg tgtccttcta attacttttc
    24601 ctaagagtct tgctgctaat tctcttgctg catccttgca aaacttatct aaatcaatct
    24661 ttgtaaattt ctccatcttt ttacaaactc ttttaaactc tctaaaatca acactgcccc
    24721 atctagccat tatgctttat ccttaaataa ctcaagtatt atttcttgat gatttggata
    24781 tatagctgat tctccacttc ttacatactc tttatcattt ataataagtt ttgaacctgc
    24841 tttaatttct atatctggag atataaagag tttaatagtt tgctctagct tagctaattt
    24901 cccttctgta gcagaaacta tatttttata tgaaagttta catggttgat tttctaatac
    24961 aatcacttct ttattgttag ttcgttttgt tacagggtct ttaattggct gatactcaac
    25021 tatagtacat ttatctctat ataacatttc tattgctttt ctagttttac ttaccatctt
    25081 aagcacctaa aggttaatat cttattctta ccataagtag taagataagc tattaagcta
    25141 tcaaagcgtt gttctggtgt ttgagaacca cttcctatag caaaatctac ctttgtatca
    25201 ccttctgata tagacttttc tacagcttca aagttaatgc tttctatatc taattgaccc
    25261 atatttttct tggtaaataa gaactctcca actatcatat caacttcaat ttctttcaat
    25321 tcaattggca tagtttttat attacaatct agtttaataa tattttctat tttttctctt
    25381 acaaaaccta ttaaccactt atctccatct tttaatatat atccaaaact ttcaagtctt
    25441 ttttctattt catcaattat attattttcc ataattttca cctacttttt agtaagttta
    25501 tttttctctt taagctgctt attttcttct tctaaagact caacttttga ccttaaaata
    25561 ttattttcag ctattaaatc ttttacactt aatgacttgc catactttac tgccttacca
    25621 gtttcatcta tcaaatcata tcccatctct aagaaatcat ctattttaca ttcttctatg
    25681 gttagtattc tatttagttt ttttacttgt ggcactatat atcactcctt tttctatgct
    25741 tcaacaacaa attgtattgc atcagctttt ttatttaata taaatacatc ctcaaaactt
    25801 tcttcaaagt agaagtattt tccctctgta actgctgttg gttcgtctaa cttagagaac
    25861 tgataagaaa caggtgtaat tattgcactt gggtgaacta aggacataaa gatttgttta
    25921 gctcctgctc ctactttcca tccagttgta aaatcatatg cagttttcat tagattagat
    25981 ggtactttaa ttattttaac tgtgtcaata tctgtggttt gtctatttaa agaagttcca
    26041 ccatctttta tgtttactgt tctttgtatc tcttttgcat ttttgataag tgtatttact
    26101 actggagtaa catacaatat tcttccattt tcaggtactc tagcttctgt cattttttcc
    26161 attaacttat caaatacttc taatacgttt gctgttgtaa gaacagttgt atctgctgtg
    26221 ttacctaatg cggtccaatc agcatatatt ttagatatac agtaagcatc catctctgga
    26281 aacttttgtt cctcattata tacttttgtt atattaccta ttgaagccac ataattagtt
    26341 tggtttatat ctgctggatg aaccaatgtt gaccatttcc tttgattagt taatacctta
    26401 ggttcccaag cattatcata gtttctttga gctactgcta ttgtatctct gtttgaatct
    26461 acccttccag ttgtagatat agttggtatt tctattgttt tagaacccgt ccatctatat
    26521 cttccattat tttctgttgc atacaaatcc ccgaagttta aagtataagg atatgcttgt
    26581 gctaaaacat ttgaatattc ttttgcataa tttagtgctg ccattttatt tcctcctatt
    26641 tattattatt ttcatgaggt cttaccccag taaaattaaa accaaaatca tttatcttag
    26701 gctcttgccc tggtgttata gtatctattt taggttcttc accttctagt gttgcattaa
    26761 acaaataatc tttatcctgt ttcaaagggt ttatttgctc ttcaaaagct ttttgtctat
    26821 ctttactatt tcttagggct tccatgtcta aatgagcttt taacgctatt tcatctctac
    26881 atttaacaga tttaaaagca tcacctaacc agtaattaaa atctttttct tcaatttctt
    26941 ttttgtaggt ttcttccaaa gttttcttat cagtttcata agttgttttt agattctcta
    27001 catcttcttt tgtcatacct ccttcaaact ttttaatagt ttcattagct gtattaagtt
    27061 gtgtttcaag atttacataa tcttcttgag taactgtagt ctcctttatt ttcttttcta
    27121 tagatttttg aagagaagct acatcaattt tgttatcctc tacttttatt ccttctagca
    27181 attcttttaa ccaatccatt ttaaatttct cctttcattt tttacaaaat aaaagcacct
    27241 actaatttat tatttagcaa gtgcttttac atatttacta tttgtatatc tttccataaa
    27301 tcctttagta atttaccatc aatgttgtaa ttatcaacca tatccttacc atttttataa
    27361 tactttgtat ctccattagg gcaaagagta ataaaccttg tatcatcatc tccaatagat
    27421 atattatatg gtttattata taagtcaaat tctatatcta atcctaaatc aatagaatca
    27481 attaaatgtt gtaaattctt aaatttatta tccattttat tctccattca aaatatcttt
    27541 gttagctatt ttatgagctt ttgtaagctc catatccttc tctcttttta ccttatcatg
    27601 gttattttca tcagctaacc aatcatgttt atgaggtaca attttatgtt gctttgggtt
    27661 tctatggtca gttaagtcta aatctaatct aggttttcct gtattaccat agtatcttct
    27721 ttgaattaat ttaccatctt tgtaattatc aaatacacta tttggttttg attcaaatgg
    27781 cactgaatga acacttccac tagttaaatt tctctgattc ttaacttgcc aatttacatc
    27841 cttataaagc tttttagctt cctcatacct tatagtatca ttatacttca tatgttgata
    27901 ttcatcaaat ttagaaggta tttcatttcc taataccttt ttatattctt caaattgttt
    27961 tctatcttta ctctcattta gttgcatttt tctaagggtg tcagcttttt cttttccaag
    28021 tctaccctct atatgtttct cataccactc attatacttc atactagatg gtacataata
    28081 tgtttttcca tcttctcctc ttgctgctct atagccttct tcatcctcga accaaggagc
    28141 tgttgttgtc ctacaacgac aatgaaatgg tggagctgtt attccaactt gataatcttt
    28201 catatcaaat atttttccat ctaactctct gcatatattt gaagttctta aatctaatgt
    28261 agcaataatc tcatatttct ctacatctaa atcactaaaa caatcttttc tacttgctga
    28321 tgcaaagaaa gctgattcag tcattatcaa attcttagct tgtgatttag atacattaaa
    28381 tctcttagca aagtcattta ctagattttt tggattttca cctctaataa ttgattgagt
    28441 tagcttagta tgtagttcat ttattaaagt aggtctatgc ttaccccaaa ttctttcact
    28501 aaagtttaat ccatctgttg cccatggttt agagataatt ttatttattc tattagtatc
    28561 aagactcatt aaactccaac caacgtttac tccttgttga acattaaaag ctgtatgata
    28621 gtatccactt gtataaatat ctctcattag tttatcaata ccatcaagtt catttccata
    28681 taaaacttct acgtgttgct gtatttgtaa ctttaaagct tcaagccttg ttatatgaag
    28741 tcttgcacta gcattttcta actccttcat ccacttttga tttatagcat tttctttgcc
    28801 atatttaata tactcttcta cattccactt aaattcttct aattctcttg tatttaacag
    28861 tttcttagct tctaataaag atattccttc attttcagca aatctgttat accatgataa
    28921 tatatctttt tctatactat tcatagctaa tttgtatttc ttttccaatt caagataata
    28981 ttttacactt ttattgtttt gaacttcttc taattgttca aatcttttcc tccaataatc
    29041 tttatgtttc atctataaca ccctcttgat tattagtagg aattaaatca tcatattctt
    29101 tttgggtttc ttcctgtttt ttaagtcttt caagttcgtc attaacatct tcaacccaag
    29161 gatgattact aacaatagtt tcatctgata taattccaat tgatttagaa gccatatcta
    29221 ttttttcaga ctcatttatt atcatagaat gattaaatgt tatttgaact gacttataat
    29281 catagctctt actaccactt atctttaaat actcacacac aaaccataaa agctctctaa
    29341 ttgctttttt aaacttcttt tcagtcttgg aacattttaa atcaagtagt gaatataaaa
    29401 atttaagagc tacacccgat ttgtcacctg tgttttgaga ttcgggatta actccttgac
    29461 caaaaataat tatattcttt tctaatctat caagaagctc ctttttggct tcaactggta
    29521 tatttatctc tagtttatca actccacctc caccatctac tttaattgat ttatagtatc
    29581 ttatattatc tataaactct tgtagacttg ttcctggata ctcttttaat acataaatga
    29641 cctcttgcat ttcatctaaa ttgtctgcaa gtgtagaaat gttattatca tatatatcaa
    29701 ttaaagactt atagaaagtt aaatctgaga cacacttttc attattttta aaaggtataa
    29761 atggaacttt accccatccc tgttctttgt tatttattct aaaatgacct tcttgtatat
    29821 cagtcatttt tccatattca tcatataaaa attcttgaat aaaactatta cctctttcaa
    29881 taaagtaagt tacgtcattt tctgtgtagt actcaactct ttttatttta tttccatcta
    29941 tatcttcaat ataataaaac ctaataaatg caactaattc cctctgtctt ttactatccc
    30001 aaataggaat tgcttcttca gctggaatta ttacatattt aaactcacct tttctattaa
    30061 tatatggatg taaccattca acccctttat tactagcatt gagatatagt tctgttattg
    30121 tatcgtcaaa ctcttctcct agtaagtcat ttaaaagctt agtgagatta tcatcatctg
    30181 cattaaatac tatgggattt ccgacactat agcctacctt ttggtcaact aaaagcttat
    30241 ggtagttgtt aattgcttta ttattaactt tagtaaaatc atcaacctta gctccatcta
    30301 agagataata tcttctctta ttgtttacat cagtattacc ataatagtat tcttctcctt
    30361 gtttatattt ttctggccta tgctttaaga tgtagtgttc tatgactttt actaggttaa
    30421 aggtgctctc tttttttaac tgaactttta ttaaatctgt ttcacttata taaatattta
    30481 acacctcctt tactttaaga agcttattcc attattttta agcttattat ctatagaata
    30541 tctaagagca gccattgcat catccataaa ctcaactggt tcatcaagat ataatccagt
    30601 tctttcgtct tgtttccatt tccattgttg tatttctttt atggtattag tgcaactagg
    30661 atgtacatgt attcttaatt gtttcaaata atctatttga gctttaacac ttcctggtcc
    30721 ttttttaact cctttagctt tatatcctgc actcttccac atcttaattc tatctggttc
    30781 agcactatca cagtacataa atagagtctt ttctaaacct ctactatttg caatttttat
    30841 gatttctgag gtatccattt catgtacata tatttcatta catatatata actctccatc
    30901 cttaaagcca attctaagta ttgcatttgc atgattaaag ccaaagtctt gtgacagtct
    30961 catattgtca aagtattcaa attctgtggg aaattcatgt ataacataat ttttaagtat
    31021 tgctccacca gtttctcccc attctccaag accatatact ttgtaccctt ctgggtcttg
    31081 ctcttttctc atttgcattc ttctgtagta agcttcatct atgaatctat tttgtagata
    31141 agtactatga tgagtaaata tatcatcatt tttatagtca aaatactttc tttttatcca
    31201 atgagtagct gagactggat taaatgtaaa tgtcatttga taatacaagt taggattagt
    31261 taaaatacct cttaaacggt catctagtat atctatgtca ctttccataa gttctgtagc
    31321 ttcttcacac caaacccatg ttaattttcc tttcgagaag ttaattgatt ttaatttttc
    31381 tctttgtttt gcatcattaa ctcctctgaa aattatagag ttaccagtaa ctttactctt
    31441 aatttctaaa ggatttaaag tagttttcca atacttatca gcttgtttac cataaatacg
    31501 atttatagct cctgtaagct ctgcatacgt tgaatactta tgtgtagctt ctgactttct
    31561 aactactaat agattagctc cttgatactt cttatctcct aactttagta tatagtcttg
    31621 tgctacatta acagattttc cactccctgc tgaacctttc attgctctgt atcttttttt
    31681 agtaaaatta gcttccttga aatctggatt aaaatttatt ctaactatca tttctatcac
    31741 cataatctac acttattttc aactcatcat ctccaatatc atctttattt aggttagcaa
    31801 cctcacattt caacttctca actcttgttt tctgctcctc tgtagccaaa ttccaatcct
    31861 tatgaatcat ttcatcatac tgcttaatta aactccttaa ctcactcata gctctactct
    31921 gtgcattaag aaaagatgct tgcctatccc atgcaaattg aaattcatac tctatcttct
    31981 cactattttc tgtgatttca tgtttcttta attccttaat catttcctct ttatctttaa
    32041 catacattat cttctgtgct cttattattg ctgcgtattg aattgttatc tgttcccaaa
    32101 gaatatcaaa tttatctttt atagatattt cttgtattaa ttccctagtt tcttcaggta
    32161 agtattttga gaagaaacca aacttttcag catttttatt ctcttttgga gcaccataac
    32221 caactgaatt tttattagaa aagggtgcac cccttttatt tataggtgca cccttctttt
    32281 tttcactaga ccaattgtat ctttttatcc atgactttaa agtatttaaa ctaatgtcat
    32341 actttgctga tatttccttt tgtttcattc cttttaagta atcttgtttt accttttctt
    32401 tgacatcttg cacatcacca cctctttatt tgtttgtttt gggaataaaa aaacaatcct
    32461 atttaatagg attgtttcaa atctagtctc ctctatcctt ccaggataaa tgaaacatac
    32521 ttattattaa tgtagttaga gataaaatta tatgaactgt aaaaaatatt aaaacataga
    32581 aaattatatt agaataatat cgagtaagca ttattgtatt gataaaacat attacaacaa
    32641 ccaaaatctc cattattaca gtataatatg cttttaactg tatgaattga aagtatgata
    32701 tattaaatcc atttaccttt ctttctgttt ttatatcttt tgcttctgat attgatttgt
    32761 tatatgtagt aaacaataat gtaataatgc aaactccaaa agctactaat agtcctgaca
    32821 aagttagtac atctttgttt aaagaaatta caacttcttt attaattgac gaattaaaaa
    32881 acatgtatga aataatacat attaatactg gtgttaaaac taactctact acttctttag
    32941 aatttatagc cttgtaataa ttgagtaaga tatttaatac ctttttatac attttaacac
    33001 ctccttagca atctatgctt cactcatagc aacttcttga tacattaaat taaaaaaaac
    33061 tgaattattc tcaattaaat catctatcaa attattcatc ttctcaaaaa tgtcttcact
    33121 atctatatga ttatctatat caagagaaac ctctatttct ctacacaact ttataccctc
    33181 agtatccaac tttaattttc tcttattttt ctctccctca attattatcc tatttatttt
    33241 tttatttcta aattcctctc ctttaatata tttttcgcaa catcttttaa cttcactagg
    33301 taaatatcta gttctaaatg tgggcttata tatcaaatcg ttatgtcgtc ttgaattatt
    33361 taaattagaa aacaaattat cttcatcagt tccaaacttc tctctatcaa catttaaagt
    33421 tatttttgaa acaccatcta aatctaatat ttgttctata aaatctttac ttgctatttg
    33481 tgtaatatta attgatgttt ttgacatatt attatcaaat ttattatttt caatcatatc
    33541 ctcataatat agatgtaagt ttttacttag ttcaatcatg ggaaccccca caaaaactct
    33601 ttcaaataat actactgcta tattattttc cttcataaat ttaataaata ggtgctgctt
    33661 attgctatct ccttgatcca tttttttatt accctctact tctttagtat gagcatttat
    33721 aatatttgta
//
LOCUS       DAEPOG010000021        33667 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEPOG010000021 DAEPOG010000000
VERSION     DAEPOG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.021"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(57..902)
                     /locus_tag="KR432_000903"
     CDS             complement(57..902)
                     /locus_tag="KR432_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639456.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            1119..2285
                     /locus_tag="KR432_000904"
     CDS             1119..2285
                     /locus_tag="KR432_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBH0639457.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            2307..3953
                     /locus_tag="KR432_000905"
     CDS             2307..3953
                     /locus_tag="KR432_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBH0639458.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            4050..5423
                     /locus_tag="KR432_000906"
     CDS             4050..5423
                     /locus_tag="KR432_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639459.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            5868..6821
                     /locus_tag="KR432_000907"
     CDS             5868..6821
                     /locus_tag="KR432_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBH0639460.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6859..7821
                     /locus_tag="KR432_000908"
     CDS             6859..7821
                     /locus_tag="KR432_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBH0639461.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7846..8832
                     /locus_tag="KR432_000909"
     CDS             7846..8832
                     /locus_tag="KR432_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBH0639462.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8845..9891
                     /locus_tag="KR432_000910"
     CDS             8845..9891
                     /locus_tag="KR432_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBH0639463.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            10058..11788
                     /gene="lpdA"
                     /locus_tag="KR432_000911"
     CDS             10058..11788
                     /gene="lpdA"
                     /locus_tag="KR432_000911"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0639464.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            12206..15154
                     /locus_tag="KR432_000912"
     CDS             12206..15154
                     /locus_tag="KR432_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639465.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            15132..15596
                     /locus_tag="KR432_000913"
     CDS             15132..15596
                     /locus_tag="KR432_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0639466.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            15709..16011
                     /locus_tag="KR432_000914"
     CDS             15709..16011
                     /locus_tag="KR432_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639467.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            16073..17437
                     /locus_tag="KR432_000915"
     CDS             16073..17437
                     /locus_tag="KR432_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0639468.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            17472..17945
                     /locus_tag="KR432_000916"
     CDS             17472..17945
                     /locus_tag="KR432_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639469.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            18117..18767
                     /locus_tag="KR432_000917"
     CDS             18117..18767
                     /locus_tag="KR432_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0639470.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18941..19873
                     /locus_tag="KR432_000918"
     CDS             18941..19873
                     /locus_tag="KR432_000918"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0639471.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            20147..20860
                     /gene="ispD"
                     /locus_tag="KR432_000919"
     CDS             20147..20860
                     /gene="ispD"
                     /locus_tag="KR432_000919"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH0639472.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20857..21342
                     /locus_tag="KR432_000920"
     CDS             20857..21342
                     /locus_tag="KR432_000920"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH0639473.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    21437..21645
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21709..23424
                     /locus_tag="KR432_000921"
     CDS             21709..23424
                     /locus_tag="KR432_000921"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0639474.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            23546..24991
                     /locus_tag="KR432_000922"
     CDS             23546..24991
                     /locus_tag="KR432_000922"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0639475.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            25431..26912
                     /locus_tag="KR432_000923"
     CDS             25431..26912
                     /locus_tag="KR432_000923"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH0639476.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            27133..27288
                     /locus_tag="KR432_000924"
     CDS             27133..27288
                     /locus_tag="KR432_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639477.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            27433..28830
                     /locus_tag="KR432_000925"
     CDS             27433..28830
                     /locus_tag="KR432_000925"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH0639478.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            28815..29228
                     /locus_tag="KR432_000926"
     CDS             28815..29228
                     /locus_tag="KR432_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBH0639479.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            29335..29430
                     /locus_tag="KR432_000927"
                     /pseudo
     CDS             29335..29430
                     /locus_tag="KR432_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            29736..30566
                     /gene="thyA"
                     /locus_tag="KR432_000928"
     CDS             29736..30566
                     /gene="thyA"
                     /locus_tag="KR432_000928"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBH0639480.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            30579..31073
                     /locus_tag="KR432_000929"
     CDS             30579..31073
                     /locus_tag="KR432_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBH0639481.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            31100..32476
                     /locus_tag="KR432_000930"
     CDS             31100..32476
                     /locus_tag="KR432_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0639482.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            32452..33552
                     /locus_tag="KR432_000931"
     CDS             32452..33552
                     /locus_tag="KR432_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0639483.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            33598..33667
                     /locus_tag="KR432_000932"
                     /pseudo
     CDS             33598..33667
                     /locus_tag="KR432_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
BASE COUNT        13668 a         3491 c         6298 g        10210 t
ORIGIN      
        1 aaattttaag gtaaactttt tttattataa agataaataa catataacct catattttag
       61 gtagattttt ttcttatatg tttaggagtg catttaaatt tttccttaaa aattctgtaa
      121 aataattttg tattagtaaa tccatgagct tctaatatac tgcataaggt atcatttgtg
      181 ttaattaagt ctttataaat atgagaaata cgcacatcat ttaaatactc taagtaagtt
      241 tgtcccatat actgtttaaa ttttctacaa aaatattctg gctgtaagtg tgctacctta
      301 gcgattttgt taatagaaat aggcattttg taattaatct ttgtatattc taaaactggt
      361 tcaaattttg acaaatcaag tactgttttt ttcatatttt tattacctac agatatttta
      421 aagtttgtat ataattcaaa taaaagttca aataataagc ttgtaaagag cagattagct
      481 gcaggaggag agctaagttt aatttctctc atgttttcta atataagttt aactttacct
      541 gaattttttt tatagttatt gtcattggca ttaaccttaa agtcaaaata gcaattttta
      601 aaatcaggga tatatttgtt tagaaaagac aatggtactt gcaataatat agaggtatct
      661 tgataagtac atctagttga gtgaataatt ccagaattaa tgaacataca ctcattagct
      721 tttaaattat aagtgtaacc ctccatgtta atttgtaaat caccagaagt tatataaatt
      781 atttctaatg agttatgcca atgatttgct acataactac cactatcatc aaatagtttg
      841 aaattaacat ctaaattatc aataggttca attatttcat gtgaaaaatt atttctgttc
      901 ataataatat cctccttata attttaaatt ataaatagta aatttataat gttcaatatt
      961 aaaaagataa tatagactaa taaattagat aataaacata cttaagaaaa cggataaaat
     1021 caaatataat aactgtatat taaaataact taaaaaaatt attttattat aagtgcaaaa
     1081 tatacctatt taaataagat aagaggggag gattttatgt gtatataaaa ggtgtaaatt
     1141 taggaggttg gttagtttta gaaaaatgga tgacctcaag tttatttgaa ggcacagaag
     1201 ctgaagatga atactattta ccaagacagc tttcaagaga ggcatatgaa tctagaatta
     1261 agactcatcg tagtgaatac atcacagaga gagattttgc aacgattaaa tctatgggat
     1321 ttaattcagt tcgtatacca gtaccatact ttatttttgg agattgtgaa ccattcatag
     1381 gatgtgttaa agaacttgat aaagcatttg cttgggcaga taagtatggg cttagtatat
     1441 tgatagactt acatacagtt ccaggaagcc aaaatggatt tgataatgga ggaatatcag
     1501 gtatatgtag ctggtcacaa aatccagaat atgtagcgtt tacattaaat gtattagaaa
     1561 gacttgcaaa acgttatggg atgagacatg aattgtatgg aatccaaatt ttaaatgagc
     1621 ctataacaga aagaatgtgg aacataatga atgttccaaa tcggtttaag gctgtagata
     1681 aggagatggc aagggggagt aaacctaatt cattagagtt tttacgtgat ttttatataa
     1741 aagcatataa ggtaatgcgt ccttatatga gagaggaaaa cgttatagtg tttcatgatg
     1801 catttgaatt aaaagcatgg aaagacttta tgagagagga agaatttaaa aatattgttc
     1861 tagatacaca ccaatatttg atgttagctg aatcagatgg atgtgaacaa agtattgata
     1921 gctatctcaa atatattaga gaaaattatg ctaaagatat tttacaaatg caaaaatatt
     1981 ttcctgttat atgtggggag tggagcttat ttaattcata tgcttgtggt atagatacaa
     2041 atggaggtca atcacctcta aatgggattg aatcaaatat agataaatta tcaaaagacg
     2101 ataaaagaga attatatagg aagatagcaa aagcacagct tgatgcatgg agaaatggaa
     2161 gtggtcacta ctattggaat tataagttat tactagatac agttaatgaa gaaggttgga
     2221 taggatggga tagttgggac ctaggcaaat gtgtagctca agaatggtat cctatagaat
     2281 attaaactaa aattggagga aaagatatga ttaaaaaccc tatacttcca ggttttaatc
     2341 cagatccttg tatttgtaga aagggagatg attattatct tgtagtttca tcttttgaat
     2401 ggtttccagg tatacctgtt tatcactcta aagacttaaa aaattgggag ttatatactc
     2461 atattttgac agatgaaaca aaaatagatt tgaagaagct tccttcatct aaaggaattt
     2521 gggcaccttg tttgacttat tgtgaagaag aagatttatt ttacatagtt tatggaatta
     2581 tgaactctat gaatgcaaga tattttgatg ttgataatta tttaattaca tcaaaagata
     2641 taaagggaga gtggagtgaa ccagtatata tacactcatc aggttttgat gcgtctattc
     2701 ttcatgatga tgatggtaaa aagtggattg catcattaga ctgggagact agagaaggat
     2761 atgaaaaacc tggtgttata tgtttagtgg aatactgtac aaaaaagaaa gaaatagtag
     2821 gatatccaaa gagaatatgg tcagggggaa ctgatagagg ttgtattgaa gcaccacata
     2881 taacaaagcg tggagattac tattatataa tgtgtgcaga aggtggaact ggatatggtc
     2941 atagtgtaac tatgggaagg gctaaaaata tatggagacc ttatgaaaaa gattcaatga
     3001 atccaattgt aacttcaatt ccaggcgatt tttatgaaag acatgaccct gaccatctaa
     3061 agcctaaata ctataatcca gaatcaaaac ttcaaaaatc tggtcatgga agctatatag
     3121 aaacaacatc aggtgaagta tatttagttc atctaacatc tagaccattt gtaccagagt
     3181 taaggtgtac tttaggtaga gaaacagcta ttcaaaaaat gaaatggaca aaggataatt
     3241 ggcttcgaat ggaagatgaa agtaatttgg caaaagaata cgtatcagaa agtaaactag
     3301 aagaacattt agtttcatct ataccaagtt ttgatgactt tgattcaaat gtattaggtt
     3361 tacagtacta tgctcctagg atttcaccat tatcatttgc agatgttaag tctagaccag
     3421 gatatgtaag gattagaggt caagaatcaa gaacatcatt aaataaagta agcatattgg
     3481 caagaaaatt aacttcagta tatgcaagaa taaccactaa gatggagttt tacccagagg
     3541 tacatcagca tagtgcgggt ttgataatgt attatgataa tatgaattat ataaatttaa
     3601 gaaagtatta tagtgaaaca ttgggtcaaa gtgcactatc aataatacat cttgaaaatg
     3661 gagagaagac ggaattttta aatactagga taccaataaa agatatacca atatatcttc
     3721 gtctgtatat acaaggtcgt aagtcatatt ttgaatggag ttatgatgaa aaaaactacc
     3781 aaagaatagg aaaggtattt gatacaacta agttttcaga tgagtactgt aaatatggag
     3841 aatttacagg tacgtttata ggattaactt gtgcagatcg tgtaaagcat aaacactatg
     3901 ctgattttga tttttttgaa tatatagcag atgaatcaaa agatgtcgat taataaaatc
     3961 attactaaaa ttttcagagc aaaggtaaat cttatgctct gaaataagaa acattagaag
     4021 gcaaatctta caaaaaggag tatggtatta tgaatacaga agttaatgtt aaagcaacta
     4081 cgattagata tgaaaagatt tcattttttg aaagaatttc ttatggttgt ggggatttgg
     4141 ggtgcaatat catatactca gctatgtcag catttttgct gttttattat actaattatg
     4201 cagatgttag tgcagcagct gtaggtagta ttatgcttgt atctaggata ctggatgggt
     4261 ttagtgactt aacaatggga attatagtgg atagaactaa atccaaatac ggtaaggcta
     4321 ggccttggat tttgagaatg gcgattcctt ttgctatagc agcagtatta ttattttctg
     4381 ttccatcaaa tcttggtatc acttctaaat taatttatat ttttattaca tataacttag
     4441 tttctactgt tatttataca gcaatcaatg taccctatgc aactctaaat tcacttataa
     4501 ctcaaaacca gtatgaaaga ggagtattaa gtatatttag aatgatatta gccacttgtg
     4561 gtaccttact aataaatgga cttacactac ctctagttga atactttgga aataatttat
     4621 cagcatggac taaaacattc tttgtatttg gaatagcatc tataatggta ttttttataa
     4681 catttactgg aaccaaggaa agagtaaaag cagttaagca agataggaat gaagtaatac
     4741 catttaaaat aggtattaaa tctttattta gaaataagta ctggatacaa ataacattat
     4801 gtttagtttg catatttatt gtatttgcga taaatggtgg ttcatccgta tattatgcaa
     4861 agtttatttt gggagatgaa aaattatttg gtcctataaa tatggtttca aatatatccc
     4921 aaattatagc tatgtttatg gttgctcctt ttataaaaaa atttggaaaa agaaatgtat
     4981 taatagtagg ttcaataata ttaatatcgt caaatgttat gtttataata gttggacaaa
     5041 actatatagg agtaatattt gcaagtgcta taaagggaat aggaagtgct ggaatagcat
     5101 caacaatgtt tgctattgtt tcagatacta ttgaatatgg agaatggaag actggatata
     5161 gaactgaggg gcttataaat agtgcatcta gttttgggtt taaagtggga aatggattag
     5221 gttcagcaat attaggtgca gtattatcaa ttggtggtta tgtaggaaca tcagctactc
     5281 aaagtgattt agctatagta tcaataaaag tatgttttat atacctacct atatttataa
     5341 caatattaca aattatcata atgttttttt ataagcttga taaagaatat agtactatac
     5401 ttaatgaatt aaatactaaa taatgaaaaa gtttcttata aagtcttaag tttaattaag
     5461 gtctattgta aatgttccct ataaaaacta ttatatattt atgattattg aagaattttt
     5521 agagaagcga ataataaaaa tataagaaga ctttaaatga tgaaatggtt ggtcttttag
     5581 aaatgattta ataagttatt tttatttaaa gcattataag tggacaatta atttttaatt
     5641 tatggagatg atatttaaca attagaagta atatataata tgcgctaaga agcaattttt
     5701 caactattta tgaaggtggt ggtacaagaa aagtatcacc ttttttttgt tatacaaaaa
     5761 aacagttgac tttttatatg aaccccttct ataatgttac tgaaattaag taaaatgatt
     5821 gaacaaatgt aaagataatt gaaaagcttg attttggagg aaatcaaatg aaaaaaattg
     5881 gagacttaag attaatgatg aagtgttgtt cactttatta cgaagataat ttgaatcaac
     5941 aggaaatagc aaatcaattg ggaatatcaa gaccaacaat atcaagaata ttaaaagaag
     6001 cttttgaaca aggaatagta aaaatacaga tagttgatgt tttgaaaaat gattaccaaa
     6061 aaatagagag aagtttggag agaaaatata aattaaaaga agtaatagta gtagatgata
     6121 aacaagatgc actaacacaa aaacaagaac tagcaagagc tgtatctgaa tatttaacta
     6181 gagtagtaaa ggaaaatgat ataataggtg tttcaatagg aacaacacta aaagaaatac
     6241 caagatatgt ggaaaaaagt aactgtaaaa atgttacatt tatacctcta ttaggaggga
     6301 ttggagacaa tgaaatagat attcatgcaa atcaaatagc agtaagtcta gcgagggctt
     6361 ttggaggtga ttttaaatta ttacatgctc cagctgttat gtctgacttg tctacaaaag
     6421 agaaactttg caaagacgaa aaaattaaag aagtactaga tttaatagat aaaacaacta
     6481 tagcaattgt gggtatagga aatcctatga gtttaaactc aacaataatg gcttctggat
     6541 acatgaatga agcagatata gaagatttaa aaaaatacaa ctcaataggt gcaatttgtt
     6601 tacaagcctt tgataaagaa ggaaaaactt ctatacttga atttaatcaa agagtattag
     6661 gagtgaaact ggaagattta aaaaagataa aaagaacaat aggtgtcgct tcaggagatg
     6721 aaaaaataga ggcaataaag gcatcattaa aagcgaaatt tataaatagt ttagcaatta
     6781 atcacagttt agcacttaaa ttattagaag attgtgacta atagaaagat agataaaagt
     6841 taacaggagg gaatgtatat gtcaatatca aaagaaacat tgcttgaaat gtataagaga
     6901 atgaatcagg ctagaaaatt tgaagagaaa gttagttggt tttttgcaag aggtatggtt
     6961 catggaacaa ctcacttatc tgtaggtcaa gaagcgtcaa gtgtggcggc tgtaatggct
     7021 ttagaaaaag gagatttagt atcattaact catagaggac atagtcaatt tataggaatg
     7081 ggaatagacc taaataaaat gatggctgaa cttatgggta aagaaactgg attttgtaag
     7141 ggaaaaggag gctcaatgca tatagcagat attgagtcag gtaacttagg agcaaatgga
     7201 gttgtaggtg gaggtcttac aatagctcca ggagctgcac ttacacaaca gtataaaaaa
     7261 acaggcaaaa tagttttatg tagttttggt gatggagcat caaatgaagg tacttttcat
     7321 gaaggaataa atttatcttc aatatggaaa ttgccaataa tattttactg tgagaataat
     7381 ttatacggta tgtcaacaag cataaaacgt catatgaata tagaaagtat tgccactaga
     7441 gcagcttctt atggaataga aggaatatca atagatggat ataacccaat agaagtatac
     7501 gaaacagtac aaaaagctgc tgagaagtgt agaagaggag aaggacctgt attaattgaa
     7561 agtagaactt atagatggtt aggtcattca aaatcagatg caaatgtata tagaacaaaa
     7621 gaagaaatag aatcatggaa agcaaaagac cctattgaat ttttaaaaaa ttatcttata
     7681 gaaaataatc tatctaatga agatgaatta gataagattc aagaatttgc aaagcaatca
     7741 atagaagatg cagtagagtt tgctcaaaat agcccaaatc caaaaataga gtctttatta
     7801 gaggatgtat atgcagacta agaatggtaa aggaggaaaa taaaaatgag tacaagggaa
     7861 ttaacatatg cacaagcaat aaaggaagca atgtcagaag aaatgagaag agatgaaaat
     7921 gtaatattta tgggagaaga tataggaata tatggagggg catttggtgt atcagttggt
     7981 atgatagatg aatttggacc agaaagagta agggatactc ctatatcaga agcagctata
     8041 gcaggagcgg cagcaggtgc agcagcaact ggtttaagac ctattatgga agtaatgttt
     8101 atggactttg taacaatttc tatggatgca attgtaaatc aagcggcaaa aatgagatat
     8161 atgtttggag gaaaagcaca agttccaatg gttgttagat gtccaggtgg ttctggaact
     8221 ggttcagcag agcaacattc acaaagttta gaagcttggt tttgtcatgt accaggagta
     8281 aaagtagttg caccttctac accagcagat gctaagggat tgttgaaagc agctataaga
     8341 gataataatc cagtcatatt tgttgagaat aaattgttat acagaaaaaa aggttttgtg
     8401 ccagaagatg attatgtaat tgaaattgga aaggcagata ttaaaagaga gggtactgat
     8461 gttacagtaa ttacttatgg aagaatgtta caaagtgttg aagaagcagc agagactcta
     8521 tcaaaagaaa atataaatgt tgaaattata gatttaagaa ctttatatcc attagataaa
     8581 gagactatag taaagagtgt atgtaaaact gggagagttt taatttgcca tgaagcagct
     8641 aagactggtg ggctaggtgg agaaatatca gccctgataa cagaaagtga atcatttgat
     8701 tatttagatg ctccagtaaa aagaatttgt ggaaaagatg tgccaatacc atataatcca
     8761 gagttagaaa aagcagttgt cccaagagtt gatgaaatag aagaagcaat aaaatcttta
     8821 attgttagat aaggagggat agatatggtg gcaaataaaa taaaagccac accagcagct
     8881 agaagtcagg caagaaaaga caatataaaa ttagataggc tgattggaag tggagaaaat
     8941 ggaaggattc atttagtaga tgtgttaaat tatttaaaag ataataaagc aaatactact
     9001 cctttagcta gaagaatagc agaagattta aatatagatt tagaaactat tgtaggtact
     9061 ggatatagtg gaaagataag aaagtgtgat gtggaaaaat taactgcaaa agaaactatt
     9121 gtatcaaata atacatctaa atatagtgag aaaaaagagc tgaaaattga aaatgaaaat
     9181 tcaagcatgt ttaacacagt agaaggaata tttgaaaagc caaatccaat gagagctact
     9241 gtagcaaaac gaatgtcaga aagttatttt agtgctcctg tatttacatt caatatagaa
     9301 gtagatgcta cagaattgaa ggtacttaga gcaaagttaa tcgacacagt aaaagaatca
     9361 actggagtaa aattaactat gacagattta atagtaatgg cagtatctaa aatacttcca
     9421 aatcatcaag ccttaaattc agcatggaca gatgagggta tatttagata taaagatgta
     9481 aatatagcta ttgcagttgg tttagatgaa ggcttatatg ttccggtagt aaaaaatgca
     9541 aacaaaaagt ctttaaaaga aatagcaaaa gaaagcaaag aacttgcgga aaaagtaaag
     9601 acagggaaac ttatgccagc agaccaagaa ggtaatacat ttacaataag taatgttgga
     9661 atgtatggta taactacatt tacacctata ataaatatgc cttcaagtgc aatcttaggg
     9721 gtaggtgcta cacaagataa gtttgttcca gtaaatggag aggctaaaat aaaacctata
     9781 atgaacttat cattaacatc agaccataga gtaatagatg gaacagtagc tgcaaaattc
     9841 ttaaaagatt taaaagaatt gttagaaaat cctttatcaa tgcttgtata ggttttgata
     9901 tttcgttact gatataaata gtttgaatag attatattat tataattgtt tcagtaaatt
     9961 taaagctttt atatattaag aattattttt aacagtaact atatgtgaat atgatttgct
    10021 taattaaaat atatgaaaat aaataggagg agagcctatg tcagtagaag taataatgcc
    10081 aaaagcagga gtagccatgg aagaaggtac tatagtatct tggctaaagc aagaaggcga
    10141 agaagtaaag attggagaac ctatactaga aataacaaca gataaagtta atatggagat
    10201 agagtcagaa ggagaaggta cacttgctgt aatcatacat aaagaggaag gtgaagtatt
    10261 accagtattt acagtaatag gtgtaatagc agaaaaaggg gaaaatcaag aggaagtaaa
    10321 agctaaatat ttatctggaa atgtttcaaa agaagatata gttgaagaga atcaaaatat
    10381 agaagttaaa gaagaaaaaa taaacaaaaa agagtgtaat catgattatg atgtagtggt
    10441 aataggagga ggacctgggg gatatttatc agcattaaaa gcagctcttt taggtggaag
    10501 agttgcttta gttgaagaaa atatactagg aggtacttgc ttaaatagag gttgtatccc
    10561 tacaaagact tatataaaaa cagcagaaat attagaagaa attgaccaat tatcaaagag
    10621 aggtgtaaaa gtaactgtag ataaagaaca ggacattaag aaggccataa aatataaaaa
    10681 tagagtagtt aaaaaactta cagctggtgt tggcggatta ttaaaaagta gagatgtaga
    10741 tgtgtttaat cttaaagcta gtgttaaaga agagcataaa gtaattttat cagatggaaa
    10801 agttttagat acagaaaata taataattgc tacaggttcc aaagtaagga tattacctat
    10861 aaaaggaata gaatcaaatt taataataac tagtacagaa gcattagatt tggaaacagt
    10921 gccagaagaa ttagtaataa ttggtggtgg agtgattggt tgtgaatttg cagagatatt
    10981 caattcaaga ggttctaaag taactatagt agaaatggaa gatagagtaa tcccaagaat
    11041 ggataaagag ttaagcgaat ctttaaaata ttctcttagt aaaaaaggta taaatgtatt
    11101 aactaaaaag aaagtttctg aatttaaaga agaaggaaat aacatattag tgtgcattga
    11161 aggtgaagtg ccaataaaag cagatttatg tctatatgct ataggaagag aagctaatct
    11221 ttctggcata gaggacttag atattaaaat agataaaggt tcaatagttg taaatagtaa
    11281 aatggaaaca agtataccaa gcatatatgc agtaggtgat gttactggag gtgtaatgtt
    11341 agctcatgct gcttttaaaa tgggagaggt agcagcttca aatgctctag gtgtgaataa
    11401 agaagttgac ttaggtgcat tacctagttg tgtttataca attccagaag tagcatcagt
    11461 tggtattaca gaagaggatg ctagaaaaaa atataatgta aaagttggta agtttaattt
    11521 tgctggaaat ggaagagctt tagcttctgg tcaagaacaa ggatatgtga aagtagtagc
    11581 agatgctaaa tatggagaaa ttttaggtat acatatgttt ggatgtggtg tagcagaact
    11641 tataaatcat gcagcatcat ttaaagcatt agagatacct actgatgaag caagtgagtt
    11701 gatatttggt catccatgta cttcagaagc tttaatggaa gctttggcag atgtaaatgg
    11761 agaatgttta cacttaccta aaaaataaat ctaaacataa attattaatt taagtctatt
    11821 attaatatta atttatatca taaaatttta aaacacttac ttagccatac cccttattat
    11881 gcaaataatt tatttgtata ataaggggta ttttttatgg aaaaatatag aatatagaat
    11941 ccttgtagtt tttgttgtac aaaaaagata gtaatataaa ataacattat cattttagaa
    12001 tgatttcaat tgtaattagg aaaagtgatt tttttgtttt taaaaaaagt attatttgta
    12061 aaatataaaa aaaaatttct taatatttga ataaaagaat agcttaattt aaatgttatt
    12121 actttataaa aaaataataa gtacaaaaaa aacaatatat ggcatataag ttgctatata
    12181 aataataagg agggaacaat tataaatgag gcaaagaata aaaaacttaa ttgaaaatga
    12241 agataagaaa aatcctttaa cagatgaagc gatttcaagt aggttaaata ttcgaagaga
    12301 agatgttacc tttttgagaa atgagttaaa aatagaagat tcaagacaaa gaagaaaagt
    12361 tgttttaatt aaagctataa aagatatttt aaaagaagaa aaaactataa ataaaaatga
    12421 tataacaaga agattaaaca gcattgggtt tacagtatca agatttacag tcatacaata
    12481 cttaaaagag attcaagatt ctggtgacct aaaacttgat aaagagagtg atgataaaaa
    12541 agaagaaata atcaataata gagaaaataa agatatagca tttgataaac tcataggtag
    12601 tagtggtagc ttaagtacaa ttataaaact agccaaagca gcaattcttt atccacctca
    12661 tggtcttcat actattatta tggggccaac tggtgttgga aagagtgagt tagcagagtg
    12721 tatgtataaa tttgcagtag aatcatccag attccctaag aatgcaccat ttatagtttt
    12781 taatgcagca gattattcag agaatccaca gcttttgcta tcacagttat ttggacacgt
    12841 taaaggtgct tttacaggag ctgatgagaa caaagaagga ttagtatcaa aggcagatgg
    12901 aggaatattg tttatagatg aaatacatag attacctcat gaaggtcaag aaatactatt
    12961 tcaactgatt gataaaggta tggtcagaaa gttaggagaa acaagactaa cacataaaat
    13021 agatgtaatg attatttcag caacttctga gccaatagat tctcatttac ttaatacatt
    13081 taaaagacgt atacctgtaa caattgaaat accagaatta gttgcaagac cattaaatga
    13141 gaggtttgac attataaata atttcttctt aatagaagct caaagaatgg gagcaaacat
    13201 acatataaag tcagatgtat taaaagctct tatgctttac gattgtatag gtaatgtagg
    13261 tcaacttaga agtgatattc aagtagcttg tgctagggga ctattaaacc aacttacaaa
    13321 caaactaaaa gaagtaaata taagcatatc agatgtacct ggatatgtaa aacaaggtct
    13381 tataaaaatc agaaattgta gaggtaaaat agaaaattat gtagatgggg atttaattat
    13441 agattcaaat cttcaaggtg agatagaaaa gaaaaatgaa gatatatata cttttccaga
    13501 tgaaatatac aaacttactg aaagaagaca tattgattta ttggaacaag gtttagattt
    13561 tgatgttata aatagaatca ttggaggaga attagaaagt tgcattcaaa aatacataaa
    13621 acaagttaaa aaagtttcta ttagtaacaa tcttccagat ataagtccaa ttgttggaaa
    13681 atatatcgta gagctaactt atgaagtaat caaaatagca tcacaatacc taagttcact
    13741 agaaccgaat ctacatttat gtttagcagt tcacctaaaa gcagcttttg agagattaaa
    13801 agaaggaaaa gtaatatcaa atgctcattt gaaagaaata ataggaaatt atccactaga
    13861 atattctgta gcagaaaagg tagctcaata tttaaaggaa agatataata taaacttacc
    13921 aaaagaagaa ataggtataa tagcaatata ttttaaaatg agtagtagaa gagatatgtc
    13981 tgaacaaaaa aagataggtg tacttcttat ggctcacgga agagtagcat cttctgtatg
    14041 tgaggttaca aataaactat taggaattaa tcacgcaagg tatttagata tgccatttga
    14101 gaagaagccg gaagaaatga ttgaagaagc tactacaata ataaaagata ttgatgaagg
    14161 tagaggtgtg attatattag tagatatggg ttcactttca tattttggag atattatttc
    14221 tggaaagact ggaattaata taaggacaat atctagattt gatacattgc tttctataga
    14281 ggttatacga agagcagttt taccagattt aacaatagac gagatagttg atgaagttaa
    14341 aggtatagag ccacaagtaa aagaaaaggt tagtaaatgt aacaatacaa aagtagattt
    14401 tagaaaacct ataattttaa cattatgtat tactggatta gggggagctt gtaagataaa
    14461 aaaaatccta gaggaaaata ttaaaagtat aactgaaaag gttgatatag aacctatagg
    14521 tatgattgaa tatccaagtc ttgatttaga gataaaacaa ataagcaatg ataaaaatgt
    14581 aatcgccata gttggaacaa ttgaccctca gatagcaaat atcccttttt ttagtctagt
    14641 agacataaat gataaagcaa agttaaatca aataaataac ctaattgata caaatcaaat
    14701 tggtgaaaca aataaaaaat atgaattatc agatttaata cataaagaag atatatttgt
    14761 ggatttacag gttaaatcaa aagaagaagt tctaaaatat atgagtaaaa aacttagaga
    14821 gaaaggttat gtgacaaaaa atttctataa aaaagtagtg gaaagagata acttatttcc
    14881 aactgaatta tcagatgaaa tagcaatacc tcacacagat ggaatagatg taattaggcc
    14941 agctataagt atagccatct taaagaagcc tattgtgtgg agtaaaagta aagtaaaaat
    15001 aatactttta ttagctgtag accaaaagtg ttttaatcca ttaagcactc tactctcatt
    15061 tatagaaaca gatgaattta agaagataaa aaacattaaa gatagagatt ttattaggga
    15121 gatgatatta gatggagtca atgaaaataa ttagtagcaa tttaattttt aaaaatattg
    15181 aagtgtctaa caatgaagat gcattaaagt ttttgggaca acgtttgttt gacgaacagt
    15241 atgtaaaaga aagctacata caagctgttg tagcaagaga aaaaaagtat gctacaggat
    15301 taccaacaga aatatacgga gtggcaatac cacatacaga tatagtacat gtaaatgagc
    15361 caggaatagc aattggaata ctgaataaac cagtaaaatt tataatgatg ggtacagacg
    15421 atactgaaat tgatgtaaaa gttgtattta tgttagcagt gaaagagcca caagaacaat
    15481 tgcaactatt agaaaaactt atgactatat ttcaagataa gaatatacta aataatattg
    15541 ttgacttaag tgaagaaagt gtgagtgatt tattaaactc taaattgaaa aattaaacat
    15601 gtaaagtata ttaaaaatgt aaaattaaac atgcaaagta tgttaaaaaa tataaaaaga
    15661 ttattaaaga tttctaacta tagatatctt taaaataaaa taatttcaat gggggtgact
    15721 aatgtgacta aaaagatatt agttgcatgt ggaacaggag tatgtacttc aacaattgct
    15781 ataaacaaat taaagaaagc acttcaagac attggtaaat tggatatggt taatattaca
    15841 caatgtaaag tagtagaagt tgcatcaaaa gcacctgatt atgatttaat aatttgtact
    15901 acacaagtat ctagtagtat aaaaacacca gtgataaatg gattaccttt tttaactggt
    15961 gtcggaatgg ataaattaat caatgatgta ttagaagagt tagaattata attaaaaaaa
    16021 ttaaggaaca tggtctctaa aatcataagt tttagagact taaaattaaa taatggaggg
    16081 tctttatatg gagatattaa atttcattgt tggtctaggt gcacaagtaa tgatgccaat
    16141 aataatatgt atttttggtt tgatattagg tacaaaacta ggaaaatctt taagagctgg
    16201 tttaactgtg ggagttgggt ttataggtct taacactatt atagctttgc taacagataa
    16261 tttaggtcca gctacacaac aaatggttaa gaatttaggg ctaagtttat caataataga
    16321 tgtaggatgg ccagcagcat cagctatagc ttttgcatca acagtagggg ctttaattat
    16381 tccaataggt cttgtggtaa atatagttat gctaattact aatactactc aaactgttga
    16441 tgtagacata tgggattact ggcattttgc ttttacagga gctttagtgg caggggctac
    16501 tcaaagtgta atgtggggag tatttgcagc agtagcaaat atggtaattg tattggtaat
    16561 ggcagattta actgctccag gtatagagga atatttaggt atgccaggta tatctcttcc
    16621 tcatggattt acacaggcat ttgttccaat tgctatagta gtaaataaat tattagacct
    16681 aataccaggt ataaataaga ttgagataaa tgctgataca ttgcagaaaa aatttggatt
    16741 atttggagaa cctttaataa tggggtctgt aataggtgta ataattggta ttgctgcgaa
    16801 atatgatata aaaggaatat tacaattagg tgtaactatg ggagctgtat taatattaat
    16861 tccaaagatg gcagcattat taatggaagg attattacca gtatcagaag ctgcacagga
    16921 atttattgag aagagattta aaaacagagg caagatttat ataggcttag attcagcagt
    16981 aggaataggt catcctgtta cattatcagt tgcactagtg ttggttcctt tgactatact
    17041 tatagcagct atattgccag gaaataaagt tttaccattt gcagatttag cagtaattcc
    17101 atttgcactt gtattaatcg taccaattac aaagggaaat gtgtttagaa cattaataat
    17161 aggaataatt ataattactt ctggattatt aatcgcaact aacttagctc cattatttac
    17221 ccaaatggct ttaaatgcta gctttaaaat gcctgaagga gcaactatga tttcaagtat
    17281 ttgtgatggt gcgaatccat taagttgggt atttgtaaaa gttatgaact ataaagttat
    17341 tggaggagta gtttttggag ttatagcttt aggaatggct atttacaata ggaatagaat
    17401 aataaatgaa aataaaaagc tatctataga agaataaggt taaaacaatt ttattaagga
    17461 gtgtataggt tatgaaaaaa tataaatatg attctaaaag gtacttactc agtgttacaa
    17521 taccaggatt ttttatgatt ttaatattga tttatgcact gtttaataac tatataaatt
    17581 ttaatttgaa tatatatagt ttgttaataa ttgtctgtac ttatggtata ttcaatactt
    17641 ttatatcatc aagtaatcct aaaaaaatta ttattgacag caaatgtata cattttacct
    17701 catttatgac tacacataag tatgaaatta aagatataga aaagataaga ataagagaat
    17761 tttataataa aaaaatttat ctaaaaatta atgatggaga cctttttaaa ggtaggtatt
    17821 ggataagaac aaatatgttt aataatagta tagcattata tagttatttc attgagttgg
    17881 aagagtgctt atatccagat tcactaagat ttagaaataa aaaatttgag aataagaata
    17941 tataaaaata aaaaattgaa ttgaagccta acttaatctt ttttgataag atataagttt
    18001 gaactttata ctaaattttt agaattttca atcaagacaa taacataata gctaagcaat
    18061 tagaacacaa ctaagatgta taaggcatgt atatgatgaa tgggaggatt taattaatgt
    18121 tattagagaa attaagaaaa gaagtattac aagcatcttt agacctttta aactataatt
    18181 tagtgacatt aacaggaggg aatgtaagtg ggagagatga acaaactggc tatatagcaa
    18241 tcaccccaag tggaatggat tatagaaatc ttacaccaag tgatattgtt atagtagatg
    18301 tagatggaaa tattatagat ggaaaatgga aagcatcagt ggatttatca gaccacttgt
    18361 atatatataa gcatagagaa gacataaata gtataattca tacacattct acatactcaa
    18421 gttgttttgc tattttaaat gaacctatag aatgcgcaag tacaactctt gcaaatgaag
    18481 tagggggttc agtaccagtt gcaaaatttt caccaccaac atttaaaaag atgggaaaat
    18541 gtgttattga agcaattggt gacaaaagag catgtctttt agcaaatcat ggcgtaattg
    18601 cagttggtcc atctgtagga catgctctta cagccgctgt aatgttagaa gattcagcaa
    18661 aagtatatta cttagcaaaa tcaattggca caccagtgct actgcctgat gaagaaattc
    18721 aaagagcgag agatgtattt tttaatgtct atggtcagga taaataaatt agataaaatt
    18781 ttaaaagtaa tatgaaaata tagatattca atgatattaa ggagatttat aatgatataa
    18841 taagtttaat agttaagaag taaaatatga aatgtaatag tatataaatt catttaagac
    18901 taaaaataat aagataaaat aaaaaggagt ctgtatttct atgtattact tattaaacaa
    18961 aagcacaaat ccttacttta atttggcatt agaagaatat ctatttttaa atgataaata
    19021 taatgatgat attataatca tatggagaaa tgaagactcc atttttatag gtaaaaatca
    19081 aaatccatat caggaagtac atcatgatgt tattgaaaag ggagaaattc ctattttaag
    19141 aagaatatct ggagggggca ctgtttatca tgatttaggt aatatcaata tgtcattcat
    19201 acaaaaagat aggcaacttc atgagataga ttttttggag catactaagt ttatgcaaaa
    19261 gatgctttca accttaggac tagatgtatc tataactgaa agaaaagatt tatttttaaa
    19321 tgggaaaaaa atttcaggct ctgcacagtc tataaaaaga aaaaattctc tttatcatgg
    19381 aactcttctt tacgattcag atttaaataa attaactaaa tatttaaatt caaataaagc
    19441 aactgaatca aatgcaacta aatctgtttc aagtaaagtt acaaatatta agtttttact
    19501 cgaaaaagat ataaattact ttttagatta ttgtgttgaa tatttaaaat ctaatataaa
    19561 gaatataaaa gaacttgaat tggatgaaga agatattaaa agtgtataca atataggaga
    19621 agataaatat agaaagatag agtggacata tggtaaaacg ccgaaatttc aagtagtgct
    19681 accatttaat aatgaaagta aaataaatat acatgttaac agatggaaag tatcaaaatt
    19741 ttttatttct tatgatgata atgtaattaa tatggataag ttgttagatt tgaatttctt
    19801 taaagaagaa ataaaagaga gtattttaga aaattatcca cattacatag atttaatcaa
    19861 tttaatattt taatataaaa cctctaattt gataaaattg tcttatatca aactagaggt
    19921 ttttaatatt cttgcatatt aacaaccatt acaaaattgt aaaattaaaa acaacactat
    19981 tgaaaaatca atatatattg taataaatat atgtgttttt cattagcaaa tagacattaa
    20041 ctatttatac aactgaaaat ttaatatata attaatcaga tggttaaaat aaaaatatta
    20101 aaagttttaa taaatgatgg ttaaaaataa aatacgaggt gataccatgt acagtgttat
    20161 aattgttgca gcgggaagcg gaaggagaat gaacttagat ataaacaaac agttcataaa
    20221 attaagagaa aaagaaatta ttgctcatac aattcaagtt ttttatgaaa atataaatat
    20281 agatgaaata gtggtatgta taaaaaaaga agaagaggat ttttttaaag aaaatataat
    20341 aaataagtat aactttaaaa atataaaaat agcatatggc ggaaaagaaa gacaggactc
    20401 aatatataat ggtttaaaaa aattagatga aaattgtgat atagtactta tccatgatgg
    20461 agcaagacca tttgtcgacc atagaataat caacgaatct ataaaggtag ctaaagaaaa
    20521 aaaggctgtg gttgtaggtg ttccagtaag tgatactata aagatagtgt cagatggaac
    20581 agtacaagaa actccagaaa gaaatttgtt gtgggcagca caaacccctc aaacctttga
    20641 atataatctc ataatagatg cttatgagca agcatataag aacaattatt atggtactga
    20701 tgattcaatg ttggtagaaa atataggtca aagtgtcact atggtaatgg gttcttatga
    20761 aaacataaaa ataactagtc cagaagattt aaatatagca gaacaaatat taaatatgga
    20821 aaaaagagat gaagttaaca gtagaaggag aattatatga gaataggatt aggatatgat
    20881 gtacacaaat taacagagaa tagaaaactt ataatcggtg gtgtagagat accacacgat
    20941 aaaggtttgt tgggtcattc tgatgctgat gtattgatac atgcaataat ggattctatt
    21001 ttaggtgctc tagcattagg agatattggg aagcatttcc cagatacaga tgaagaatat
    21061 aagggagcag atagtatgaa attattagaa catgtgtata atctaataac aagtaaagga
    21121 tataaaattg gtaatataga tagtactata attgctcaaa gccctaagat ggctccatat
    21181 attgagagta tgagaagtaa tatatcaaaa gttttaaaca cagacattga taatataaac
    21241 ataaaggcaa caacagaaga aggtcttggt tttacaggag caaaacaggg aatagcttca
    21301 caaagtattt gtttattatt gttgacaagt cagaataatt aatatattat tagtaataag
    21361 tttaatggat atcaagtaat aggcctatat agaatatcta taatgtatga atttaagtga
    21421 aattaatata aatataaaat tagctatgat aaagaaatag taaaatgatg aaatcttcac
    21481 agagagaaaa cggtggtgag agttttcaag aggaagtctt tgaacctgtc ttttagctta
    21541 aaggtataaa aatctttacg gtaggaaccg ttatattccc aaaagagagc tattcatata
    21601 gctaactaga gtggtaccgc ggaaattaaa cctttcgtct ctatacttgt atagagatga
    21661 gaggtttttt atattttcag gaagttaata ttcaggagga atctattaat gaaaatgtca
    21721 aaaatgttta tgccgacatt aaaagaaatc ccagcagatg ctgagattac aagccatcaa
    21781 ttaatggtta ggtcaggcat gataaaaaaa atgacttcag gtgtttataa ccaattacca
    21841 atggggttaa gagtgttcaa aaaaattgaa caaataatta gagaagaatt aaataaaaaa
    21901 gattgtcaag aaatactttg tgcagcctta cttccttctg aattatggaa agagtctgga
    21961 aggtggactg ctatgggtga agaaatgttt agactaaaag acagaacaga aagagagtac
    22021 tgtcttgggc caacacatga agaagcattc actgatataa taagacaaga aattacttca
    22081 tataaacaat tgccactgaa cttgtaccaa atacaagtaa aatatagaga cgagagaaga
    22141 ccaagatttg gtgttatgag aactaagact tttacaatga aggatgcata tagttttgat
    22201 gtagatgata aaggtttaga taagtcatat caagatatgt ttgatgcata tgtaagtata
    22261 tttgatagat gtggattgga gaatagccct gtacaggcag attcaggagc tataggaggt
    22321 tctacatcag ccgaatttat ggttaagtca gaagttggtg aagatgaagt tgtattttgt
    22381 agtggatgtg attatgcagc caatgtagaa agagcagaat cttgtaatct agcatcacaa
    22441 aaagaagaaa tgaaagaatt agaagaggtt catacaccag gagcagctac aataaaagag
    22501 ttagaagaat ttttaaaaac ttctccagat aagtttgcta aaactttagt atatgaggca
    22561 gatggaaaaa cagttgtagt cgttgtaaga ggagatagag aagttaatga aatcaaagta
    22621 tctaatgcta taggttctgt tatagaattt gctcttgcta cagatgatgt agtgagaaaa
    22681 gtaactaatg cagaagtagg atttgcaggt ccaataggta taaatgctga ctatgtattt
    22741 atagataaag aaattgttga acaaagaaac atagttgttg gggctaataa aactgagtac
    22801 catataaaaa atgctaacta tggaagagat tttgaaggaa tagttggaga ttttagaaat
    22861 gttcaagaag gcgaccagtg tatagtatgt ggtaagccac ttgaaatagc tagaggtgtt
    22921 gaagtaggac atatatttaa actaggaact aagtactcag aatctatgaa tgcaaacttc
    22981 atagacaaag atggaaaatc aaagcctata gtaatgggat gttatggaat aggtgttgaa
    23041 agaacagcag cagctataat tgaacaacat aatgatgaga aaggtataat atggcctttg
    23101 tcagtagctc cataccatgt tgtgatagtt ccagcaaata tgaaaaatga agaacaaata
    23161 tcaatagctg aaaatatata taatgatttg caagctatgg gtgtagaagt tttattagat
    23221 gacagagatg aaagaatagg tgtcaaattc aatgattcag aattaatagg aatacctatg
    23281 aggattacag taggtaaaaa tattaatgaa ggtaaagtag aatttaaact tagacataaa
    23341 gaagataaag agataattga catagaagaa ataaatgaaa aagtaaaggc agagtttatc
    23401 aggaataatg ttaggttagg ataataactt aaataagata ttgtatagat aatcaaagtt
    23461 gtactttata gacaccattt tatctatata taaggatata aaatggtgtt tttttataaa
    23521 acaaataaat gaggaggtaa ttgaaatggc aaaaaatgaa aaacaatttg ttgaagaaat
    23581 aacaaagatg gaagatgatt ttccacaatg gtacacagat gtaataacta agactgactt
    23641 agttgattat gcaccagtaa aaggttttat ggttgtaaag ccatatggat atgcattatg
    23701 ggaaaagatg caagaattta tggataaaaa atttaaagaa actggacata aaaactgtta
    23761 ttttccatta ttaataccag agagtttatt aaacaaagaa gcagaacacg ttgaaggttt
    23821 tgctccagaa gttgcatggg taacacatgg aggaaataaa aaattagaag aaagattatg
    23881 tgtaagacct acttcagaga ctataatatg tacaatgtat gcgaaatggt taaaatcata
    23941 tagagaatta ccatatcttt ataatcaatg gtgctcagtt gttagatggg aaaaatcaac
    24001 tagacctttc ttaagaacat ctgagttttt atggcaagaa ggtcatacat tacatgaaac
    24061 agcagaagaa gcacaagaag aaacaataca acaattagaa gtatataaag cgttatgtga
    24121 agaattactt gcaatgccag ttgtagctgg tcaaaaaagt gaaagtgaga aatttgcagg
    24181 tggtgaaaga acatatacta tagaagctat gatgcatgat ggtaaagcat tacaatcagg
    24241 gacaagtcac ttcctaggtc aacactttac taaggctttt gatattactt ttgcagatag
    24301 agaaggaaat ttagctaatc cataccatac atcttgggga gcatctacaa gacttatagg
    24361 tggacttata atgactcata gtgataacag aggtttagta ttaccaccaa gagtagctcc
    24421 aattcaagtt gttatagttc ctatagctgc taaaaaagga aatgtaatgg agactgttga
    24481 taaaatatat gctgatttaa aaactaaggg agtagccgta gaagttgatg atagagataa
    24541 ctatacaact ggatggaaat ttaatgagtg ggaaatgaaa ggtgctccag taagagttga
    24601 aattggacca aaagatatag aaaataatca agctatggta tttagaagag atactttaga
    24661 aaaggattct atgcctttag aaggattagc tgatgctata tgtgatttat ttgatgttat
    24721 acacaatgat atgtttgaaa aagcaagaaa acatagagaa gacaatacat ctatagttga
    24781 aaatatggat gagtttagaa aagctttaga agaaaagcca ggattcataa agactatgtg
    24841 gtgtggagat gcagaatgtg aagctaagat taaggaagaa acaggagcaa ctataagatg
    24901 cttacctttt gaacaagaaa acttaggtca caagtgtgtg tactgtggaa aagaagcaga
    24961 tagtatggta gtaatggcaa aagcttacta aaatacattt aaataaaaac tggggtagaa
    25021 agtatttaat actttatccc agtttttttg ttttttatga gtaataaaat gtatttgtaa
    25081 ctatgtcata agataagtca tagatactta tattaaaaag aatataatag agaactattt
    25141 ttaataatta catattaatg aaagcaaaat atttttgtta tatagatttt caataaagca
    25201 aacaataaat ataaatgaat attagtaagg agtgctttta atttttagaa aataaatgac
    25261 ttaaaagaaa gatttatgaa attaaaacta ggattttctg atataataaa catgtgtatt
    25321 tttaaataaa aaggatatta ttattagatt tgattaaaaa taaacttgca aaacaatgta
    25381 ctagtttaat attatagtta tattgaataa aatatcagga ggaagtaaaa atgaatgtaa
    25441 gagtaaggtt tgcaccaagt ccaacaggat ttgtacatat aggtagtttg agaactgctt
    25501 tatataacta tttatttgca aaaaaaatgg gtggagaata tatattaaga gtggaagata
    25561 cagaccaaag tagattagta gaaggtgcta tagagaatat gcttaatgct atgaaatggg
    25621 ctggtgtcaa ccatagtgaa ggtgttatat tagatgatag tggaaaagta gtacaaaaag
    25681 gagaatatgg tccatatatt caatctcaaa gattagatat ataccaagaa tatataaagc
    25741 aacttttaga tagtggaaaa gcttattatt gtttctgtac aaaagaaaga ctggatgaag
    25801 taagagatgc tcaaagagca gcaggagaaa cagctaaata tgatggtcat tgtaagaatc
    25861 tttctaaaga agaagtagaa gcgaatataa aagcaggaat accatatgtt ataagattaa
    25921 gattgccaga gaatcataca attaagttta cagacttagt tagaggagat atggaattta
    25981 atactaatga cctagatgac caagtattaa tgaaaacaga tggatttcca acttatcatt
    26041 ttgcagtagt tgttgatgac tatttgatga aaattacaca tgttataaga ggagaagaat
    26101 gggtatcatc tactccaaaa catgtttatt tatatgaagc ttttggatgg gaagcacctg
    26161 tatttgtgca tttaccaaat atacttaata aagaaaagaa aaaattaagt aaaagacaag
    26221 gtgatgtagc tgttgaagac tttaagaaaa aaggatattt accagaggga ttagtaaact
    26281 atgtagcatt agttggttgg tctcctgaag ataatcaaga attgtttaca atggaggaat
    26341 tagaaaaagc cttctctgta gaaagagtat ctaaaagtgg tggagtattt gatactgaaa
    26401 aattaaattg ggttaatcag cactatataa aagatggtga tgatgcttat ctaacagact
    26461 tagcaatacc atttttagta gaagatggat ttataacaga agaagaagct actaataaat
    26521 atgatttctt aaagagtatg atatcagtac ttaaagagaa attacaatat gtaaaagaaa
    26581 ttactgaaca tgcaagtata ttttttggag ataaaataga ggtagaaact gaagagggaa
    26641 gagagttctt aagattagaa catataccta ctttaataga tgctttgaga gaaaaaatag
    26701 aaaaaacaga ggtattaaat gctgagtttg ttcaagctat gcttaaagaa atacaaaaag
    26761 agtacaaaat aaaagggaaa aatttattta tgggctcaag aataattttg acagggcaaa
    26821 tgcatggtcc agatttacca aaagtaatgg aagttttagg aaaagaaact tgcttaaata
    26881 gaatagctta tgtaaaaaat aatatattat agtcataaaa gtccgctaat ttagaagcgg
    26941 actttttttg tgttagtatt tggtgaaaat atctgcttaa gaaaatgaag gtatttaagg
    27001 taataaattt aatatataaa aagcaaagca aaaatataaa taaaatatga ataataaact
    27061 aataaaatat taagcataga agaagggaat ctattatttt agtttgattt aattattaag
    27121 ggggtaatta ctatgtatag taatattgat gatgttaaaa aggaattaaa ggagctatgc
    27181 ttagaatatg ttacaatttt agaaaaatta aaagatgaaa aaatgattac agaagaaact
    27241 tttgaaaagt gctcttctca aaaaaaaata tttttagaag aacaatagag gatggtaata
    27301 gtattattat tatgtagttt gaaagatagt acgaaaaatg atagtaatac tattatttat
    27361 tgcaattttc aactataata taatatataa tgttaagaag ataatttata aaaatacagt
    27421 gaggtgatga gtgtgaaagt ttataataca ttgactagaa caaaagaaga atttgttcca
    27481 ttagaagaag gaaaggtaaa aatgtatgtg tgtggtccaa cagtttataa ctatatacac
    27541 ataggtaatg caagaccatt tataatattt gatactctaa gaaggtattt agagtataga
    27601 ggatacgatg taacttatgt acaaaacttt acagatgttg atgataagat tataaataga
    27661 agtcatgagg agggaatatc tcctgaagaa gttgcggcta agtatataaa agaatatttt
    27721 gtagactgtg atgggctagg gataaaaaga gctactgttc atcctcaggt tacagataat
    27781 atacaacaga ttatagagtt tataaaagag ttggaagata agggatatgc atatgcagta
    27841 aatggtgatg tatattttga tacaaataaa tttgaaggtt atggaaaact ttcaggtcaa
    27901 aaacaagaag atttagaggc aggggctaga attgaggtta atgaccaaaa aagacatcct
    27961 atggactttg ttctttggaa ggcaaaaaaa gaaggtgagc caggatggga tagcccttgg
    28021 ggagaaggta gaccaggatg gcatatagaa tgttctgtta tgtctaagag atatttaggt
    28081 gaaacaattg atatacatgc aggtgggcaa gatttaacat tcccacatca tgaaaatgaa
    28141 atagctcaaa gtgaagctag aagtggaaaa atattctcaa aatactggat gcataatgga
    28201 tatataaata taaatgatga aaagatgagt aaatcaaaag gtaatttctt tacagtaaga
    28261 gatatatcaa aattatatga tttagagata gtaagatttt tcatgttatc agcacattat
    28321 agaaatccag taaactttag tgatgagatg ttaaatcaag ctaaagcagg tcttgaaaga
    28381 ctatataata ctaaagaaaa attagaattt acgctaagta atttagtaga atctccttta
    28441 acagaaaaag aagtagagct tgttaaagaa ttagatgact ttagacaaaa attcatagat
    28501 gctatggatg atgatgttaa tacagcagac gcagtaagtg taatatttga attagctaag
    28561 ttaataaatt ctaatgtaga tgaaaattct tctttggagt ttgctaaaaa atgcttagat
    28621 gaatttaatg aacttacagg agtattaaat attgtaaata agaaaaagga tactgtatta
    28681 gataaagaca ttgaagaatt gatacaaaaa agaactgatg caaagaaaaa taaagagttt
    28741 caattagctg atgatattag acaacaatta ctagatatgg gaattgtatt agaagacaca
    28801 agacaaggtg ttaagtggaa aaggatataa aaatggaaaa aactgaatta gttactatgt
    28861 caccattagt tttagcatac ttaggtgata cagtgtatga gacatatata agagaatatt
    28921 taattagaca gaatacacaa agaaaagtta atgatttgca taaactagct ataaagtatg
    28981 taaaagctaa agctcaggca actataattc atgaaattga aattgagtta actgaagaag
    29041 agagtaaaat ttataaaagg ggaagaaatc aaaagtcaaa tacttctcct aagaatgcag
    29101 atattataga ctacaaacat gcaactgggt ttgaggcatt agtgggatat ttgtatttaa
    29161 ataatgaaat agagagactt caatatatta tcaataaagg aatcaaaatt attgaaagag
    29221 atatgtaaat attaaagagt gttgtcttaa taataagtaa taaggcaaca ctttttttat
    29281 aactaatgta taataaatat atagaaatta gtaatgagaa aggataagaa agttatgaaa
    29341 gtaaaattaa tatcacatac acctgaacca gaaaaagtaa tagctatggc agctaagcta
    29401 tgttattcac cagttgggac agacgaaata gaaaaagatt tctaaaatat attaaagagc
    29461 attcctataa caaatactat ggcggttgga atctatgaga agttatagat atagaattat
    29521 gtaaattatt tggaaaaaac aggtttgaaa attagccatg ttggtatttc taggttactt
    29581 aaggaagatc aaacataaat aagctgagtt gtcttaggtg cactatgtac aacattagaa
    29641 tgtgaagttg aagatttatt acttcaaacg aacgatggta ctaagaaaaa caatgatgaa
    29701 atgattaatt aataataaag gtaaggaggt agtatatgag tttagcagat aaaattttta
    29761 ttgatatgtg taataatatt cttgaaaatg gagtatcatc agatggagag gtagttagag
    29821 caaaatggga tgatggccag cctgctcata caataaagaa gttttgtgta gtaaataggt
    29881 atgatctttc aaaagaattc ccaatactta cactaagacc aacaaactta aaagcagcaa
    29941 tagatgaatt actttggata tggcaaaaaa aatctaataa tataaatgat ttgaatagcc
    30001 gtatttggga tagctgggct gatgaaacag gttcaatagg gaaagcttat ggataccaaa
    30061 ttggacagaa acataaatac aaagaaggag aatttgacca aatagataga gtgatttatg
    30121 acttgaaata taatccttat agtaggagaa ttataactaa tatatataat cataatgatt
    30181 taaatgaaat gaatttatat ccttgtgcat atagtatgac atttaatgta actggaaata
    30241 gattgaatgc aatattaaat caaagatcaa atgatatttt ggtagctaat aattggaatg
    30301 taactcagta tgcaatttta gttcacatgt tagcacaaat aagtgatttg gaagtaggcg
    30361 aattggttca tgttatagcg gatgcacata tatatgatag acatattcct ctagttaaag
    30421 agcttattaa tagagaatct tatgatgcac caaaattaat aataaatcca aacataaaga
    30481 atttctatga ctttagagtt gaggacttta cattagaaaa ttataaaagt gggccacaaa
    30541 tcaaaaagat tcctgtggca atttagaagg agttatttat gaaagttata gtagctgtag
    30601 atttaaattg gggaattggt tatggtggaa agttactaca gtcgattcct gaagatatga
    30661 aattttttaa agaaaaaact gtaggtaatg tagttgtaat ggggagagaa acctttaaat
    30721 cattaccagg aaaaaaagca ttaaaagata gagtgaatat agtcttgact agaagtaaat
    30781 cttttaatga tgatagattg attgtttgta attctataga tgaaactctt aaggaactac
    30841 aaaaatatga aaatgataaa atttttataa taggtggaga aacagtatat aaacaatttt
    30901 taccttattg taatgaacta tatgtaacaa aaatacagaa gacatatgag gcggatagat
    30961 ttttccctaa tatagatatt atgtcaaact gggatttgat taaagaaagt gaagtaaaag
    31021 aatataacaa tataagatat gttttttcaa catatataaa taatagttta taggtttgta
    31081 taggagagaa atttaattta tgaaaaagga tatatttgta aatagagttt ctaatgcttt
    31141 agaaaatgat agaggcgcag tttttgtagg tagtggtata tcgtttagtt cgactgaagt
    31201 tgattggttt aaattactgg agccattagt aaatgaactt gatataacat tagacaataa
    31261 aagtgatgat ttacctatga ttgctcagta catagtaaat aattattcag gaaatagagg
    31321 gcctttagta aacgaaatat caaaaacatt taatagaaag tttaaactaa acaagtatca
    31381 taagtattta gcaacaacta aactttcaac aatatggaca actaattatg atatgttgtt
    31441 agaagatgca ttttatgatt ttgatacatc ggttaaagta agtgatgatt ctattacaag
    31501 gaatagacta gaatatgatg tggaaattat aaaaatgcat ggatgtattt ctagatccga
    31561 ttggaatgaa atagttataa ctcaagaaga ttatgatgat ttttcaataa ataaaccagc
    31621 tatatctcaa agattaagtg aagatttaat aaataagtct tttctattta ttggatatag
    31681 ctatagagat ccaaatataa gaaatataat gataaatgct agaaggctaa gtaaaaaaca
    31741 tactcaacaa cattatataa ttttaaaaaa gttaaaagat agtgatagta atattacgca
    31801 agatgatgta gtaagacaag aactttggtg caatgattta aaacgtttgg gaatttcaac
    31861 tttatttata gatgaatact cagagttaga ggaactttta agagagataa gtcaaaaatc
    31921 aagaggtaaa acagtatatg tgacaggttc tcataaagaa agcaatatga atgcaagaaa
    31981 attaggaacc aagctagcaa aacataaaga tattacttta ctaagtggtc aatcatctgg
    32041 tattggctca gaggtagttg ctgcgtttac tgaagagtgt attaagagta aagaagatat
    32101 tataaataga atgaaaatat ttcctaatcc atattcagct gatgaaaaat actctgatga
    32161 tattacttta ataccagatt taaagaaatg tagaactaaa atgttgaatt caacacaagt
    32221 tgtagttgta tttagtggag gtataggtac tgaagcagag atagatgttg ctataaaaca
    32281 aaactgtaat ataattcctg ttataacatg taaagaggat agagagaatc cagctataaa
    32341 aaaaatctta aactatagtg ataatatgaa aaatatagaa ataaaagatg ctacctatta
    32401 taataaaata gtagatgaag atggtgttgt aacaatggag gaaatttata tatgcatatc
    32461 aaaaataatg aattaaaaca tgacattatt aaattgaaaa aaagaaatgg atttattgaa
    32521 gttggaaaag ataaaaaaac gttggtaaac tgtttaattg gagcgaatga attagaggat
    32581 tacaaatatg aaataaaaaa aatagattgt atacttaata tatcagaaaa accagatata
    32641 gtttcagatt taagtactaa aagaagaaaa ataagtgaat ctatatggta taagatagtt
    32701 aaagatacac ctttcgtatc tgctacactg cccatatact tagttagtaa aaataataat
    32761 aaaatagata gtaatgaatt attagatata attattgagc agatggaaaa tggagttggg
    32821 atgataacga ttcatccaac agcaaataaa caaatttttg aggattctaa gaatagaatg
    32881 gtacctgtca catctagagg tggaggtatg gtaatacaag atttaatctc aaaaaatttt
    32941 tcagaagata atgtgtattt gaaaatattg ccagaaatta taaagtatgc tcaaaaacat
    33001 aatgtttgta tcagtctagg tgcaacattt agatcaggaa acatatttga ttctaatgat
    33061 agagcacaga taacagaaat taagagtcaa atagaattag ctaagaggat atataaaagt
    33121 aatgtcgggg ttattattga atcaccaggt cattcaagac caagtgacat aattaaaata
    33181 tcgtcaatat taaaaaaaga aggtttccca attatgccac taggaccaat accaacagat
    33241 attgcaatag gaatggatca tatcgctgca gctattggtg ctacaattat gggtatagaa
    33301 ggatgtgcaa atataatatc aactgtcaca cgagaagaac atactggagg gagaccttct
    33361 attgaatcca cagttgaatc tataagaaca gcaaaaatag ctgctcatat tattgatata
    33421 catcaaatga atgatattaa cttagatatg agtgtatcta agtctagagc taaatctaac
    33481 acctgtatat ttgggaaaga tacaagatat tgtgacagat gtagagaact gtgtcctttg
    33541 tgcattagat aaaaattata ttcaagttaa ttcaataaaa aagattggat aaaaatacat
    33601 cgtatagcta gtttttcaca acagagtcaa agatatgtca aattagagca atttgaatac
    33661 ataattc
//
LOCUS       DAEPOG010000022        31695 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEPOG010000022 DAEPOG010000000
VERSION     DAEPOG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.022"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(554..1147)
                     /locus_tag="KR432_000933"
     CDS             complement(554..1147)
                     /locus_tag="KR432_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0639484.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1708..1938
                     /locus_tag="KR432_000934"
     CDS             1708..1938
                     /locus_tag="KR432_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639485.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            1995..2645
                     /locus_tag="KR432_000935"
     CDS             1995..2645
                     /locus_tag="KR432_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0639486.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            2859..3530
                     /locus_tag="KR432_000936"
     CDS             2859..3530
                     /locus_tag="KR432_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639487.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3527..4657
                     /locus_tag="KR432_000937"
     CDS             3527..4657
                     /locus_tag="KR432_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639488.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5062..5907
                     /locus_tag="KR432_000938"
     CDS             5062..5907
                     /locus_tag="KR432_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639489.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            5907..6935
                     /locus_tag="KR432_000939"
     CDS             5907..6935
                     /locus_tag="KR432_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639490.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            6938..7951
                     /locus_tag="KR432_000940"
     CDS             6938..7951
                     /locus_tag="KR432_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639491.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(8433..9683)
                     /gene="cme"
                     /locus_tag="KR432_000941"
     CDS             complement(8433..9683)
                     /gene="cme"
                     /locus_tag="KR432_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBH0639492.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(9769..10515)
                     /locus_tag="KR432_000942"
     CDS             complement(9769..10515)
                     /locus_tag="KR432_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0639493.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(10630..11652)
                     /locus_tag="KR432_000943"
     CDS             complement(10630..11652)
                     /locus_tag="KR432_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBH0639494.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(11652..14072)
                     /locus_tag="KR432_000944"
     CDS             complement(11652..14072)
                     /locus_tag="KR432_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0639495.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(14158..15078)
                     /locus_tag="KR432_000945"
     CDS             complement(14158..15078)
                     /locus_tag="KR432_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0639496.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
     gene            15688..16614
                     /locus_tag="KR432_000946"
     CDS             15688..16614
                     /locus_tag="KR432_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0639497.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(16802..17422)
                     /locus_tag="KR432_000947"
     CDS             complement(16802..17422)
                     /locus_tag="KR432_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639498.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17394..18977)
                     /locus_tag="KR432_000948"
     CDS             complement(17394..18977)
                     /locus_tag="KR432_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639499.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(19121..21148)
                     /locus_tag="KR432_000949"
     CDS             complement(19121..21148)
                     /locus_tag="KR432_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0639500.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            complement(21161..21910)
                     /locus_tag="KR432_000950"
     CDS             complement(21161..21910)
                     /locus_tag="KR432_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639501.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            complement(22207..23859)
                     /locus_tag="KR432_000951"
     CDS             complement(22207..23859)
                     /locus_tag="KR432_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBH0639502.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            24458..25579
                     /locus_tag="KR432_000952"
     CDS             24458..25579
                     /locus_tag="KR432_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0639503.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            25615..26028
                     /locus_tag="KR432_000953"
     CDS             25615..26028
                     /locus_tag="KR432_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0639504.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            26021..26560
                     /locus_tag="KR432_000954"
     CDS             26021..26560
                     /locus_tag="KR432_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0639505.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(26760..27245)
                     /locus_tag="KR432_000955"
     CDS             complement(26760..27245)
                     /locus_tag="KR432_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639506.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            28096..28473
                     /locus_tag="KR432_000956"
     CDS             28096..28473
                     /locus_tag="KR432_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0639507.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            28881..30686
                     /locus_tag="KR432_000957"
     CDS             28881..30686
                     /locus_tag="KR432_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639508.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            30763..31302
                     /locus_tag="KR432_000958"
     CDS             30763..31302
                     /locus_tag="KR432_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBH0639509.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
BASE COUNT        11547 a         3985 c         4838 g        11325 t
ORIGIN      
        1 atttaagaaa tatagaaaat taatttaaag aaatacaata cattaatttt taagtaacat
       61 taaattaatg taagtaaaat aatgttcata tgaagattta aaggtgcatt ttctgaagct
      121 aagtatatgt tatgtctcta tatccggtga agtttaatag ctcaagaaaa tgcactattt
      181 atgatgatta ttttgtttta tttccaattt tggaaataac tattctaatt aaatctaaaa
      241 ttataccata aataagaaaa gaaagtatag atgctatgta ccaataaatc gaaattgacc
      301 ctgtaattgt tctacatggg atttgcatgc ctgaatccat aaaaacaaaa aaactatcaa
      361 tggtagaaat aatagacaaa ataggattta atcctattag aataatactt tttgagtctt
      421 gaccaatttg gtggatataa atctcaaata atgcaattaa aaaaaacaaa atgtaaaaga
      481 tttaataatt tttttcatca aaatacctca cctaatataa attctgtttg cactatttgc
      541 ttactataac tatttatctc cattcagtca catcacttaa ttgtaatcta ggtcttcttg
      601 atggttcttc gtcaggataa cctaaagata ttgctgccac taattgacta tctgtattta
      661 accattcaca taattcacga tatgcaaagt atacatcaca aatccaaaga ctacctagac
      721 ctaattccac agctgtaagc gacatattct gaattgcagc tccaattgat tgcatgtttg
      781 ccatttcata gaatcgttct tcaggggata gcttttctaa aggtgattta cctaatatat
      841 ttaaaataaa aattgttact ggagcttgtt tcatgatttc aactgtgtaa tttgcaccag
      901 cgatatgttg acgacttcct ggaagaagcc cattatcatt aatttcattt tgaatacctt
      961 tgctcatggc tttaagcata gattcttttt ccttttcagt gatgacaaca aaacgccatg
     1021 gttgcctgtt tttagatgaa ggtgcattta ttccagcttc aattattttc tctatagttt
     1081 catgtgaaat gctttgattt ttatattttc tgatacttcg tctttttgat atagaatcac
     1141 ttatcatttt aagtaacctc cgtttatttt ttggaaaaat ctatttattt tctattttta
     1201 attattttag cattaatcag aaaaaaagtc attgatttta gtatacaatt attaacgatt
     1261 atattaagga ttgtatcaaa aagtatgtga ttatactaag ggattatatt agacattata
     1321 tttaaaattg tatgaaaggt tgtatttaat attatattaa gagagatata tattaggatt
     1381 tatatatatt aagatttatt atattgagcc cttatattaa attaaggatt ataatttaaa
     1441 aataagctat aaaaacccaa tattgttttg ttcagagata aaaaagaata ctttaggtga
     1501 tttttattaa aaattaagag tgataagatt atatacttta taaatgtaaa tatgacgaat
     1561 aaaaatgata aatataataa tctatatatt tacattagga gttttctata ctataattat
     1621 ttatatttaa aactaaatat aaatgttgga attatttagg atttgaaatt gtctaaagat
     1681 aaatataagt gatgaagagg tgatgtgatg agattagata tggatagtat gttatatgta
     1741 ctaatttata tgacattatt ggtatctatc attggaagta tattttattt ttgtaaagac
     1801 ctcttaaatc ataaaattaa gtatgtttta ataagagaca tatttgtaaa tagaattaca
     1861 tattctgaag aacggttgcc taaacagtat atagtttttc agaaatatga tatttggagg
     1921 tattgtagtt tgttttaatt tgaaatgaat ctattataaa ttctaaagtt taaaaataaa
     1981 aaggagagaa taaaatggat ttcatttcaa gtatattgca aggattatta agtatattat
     2041 ttaattttac aggtgatttt ggaatttcta ttgtacttat aactttaata gttaaattag
     2101 tacttatgcc actatcatta aaacaaagat tttctgtaaa aaaacagcaa gaattagctg
     2161 aaaaaatgga acatataaaa gaaaagtata agaatgatac taaagaattg gaaaagcaaa
     2221 tacaagtaag ttctgtagaa agtatgaaga gtatgctggg atgtcttata gttttggttc
     2281 aaatgccagt agtatatgct ttatatcatg tatgtttaga tatgacaaag gaatttacaa
     2341 caattataat tccatggatt gctaatttag gtacatttga taatttgttt atattacctt
     2401 gtatatatac attaattatg ttagcttcaa atttagtgaa ttatatacca tatttaaaaa
     2461 taaattctca agtaaagttt aacaaacaaa tggctgtggt gactaccata acaagtctta
     2521 tactaactgt tagaactcca gtagccatag gtctgtactt tataacaagt gcaatttatt
     2581 cattaattga agatatttgt tttagaatgt attttaatcg aaaaaataga atagtgacaa
     2641 aataatataa ttattattag aaatatttta gcctgctaac atagaaaaag ttagtaggct
     2701 atttttatct atgatatcaa ttccattaag aatagtctaa aaaatactat caaaatgcta
     2761 taaaaaatac tataaaaata ctataaaaaa atagttgaaa aataaataaa ttaatataaa
     2821 ctaaaataaa gcctacaaaa aagaggtgat aaatagaaat ggctaaaata ctagttgtag
     2881 aagatgaaaa aaggatgcaa agtattattg tagaatatat gcaaaaggga ggatatactt
     2941 gtataactgc taatgatgga gtcgaggcac ttacaatatt aaagagcaac aatatagatt
     3001 taatgatatt ggatattatg atgccgtatt tagatggatt ttcagtgtgt agagtttcac
     3061 gtgaaatgac taatatacct attatcatat tgacagctaa aggtgaagag gaggacaaat
     3121 taaagggtta tgaatatggc gcagatgatt atataacaaa accatttagc ccaaaagtac
     3181 ttttagcaaa agttaatgcc ttgttacgta gatacactac tgatattccc aaaaattcat
     3241 tgagtttagg aaagattttt attatggtag catcaaggca agtgtatgta gaagataaat
     3301 tgattgattt aacatataaa gaatttgaat tattgcgttt atttatggaa aaccctaatc
     3361 aagttttttc cagagagaag cttttaaatt gtatatgggg atatgatttt gaaggaaaca
     3421 cacgaactgt agatacacat ataaaaacct tacgcaaaaa gcttggtagt gaaggacacc
     3481 atatagttac attaattcgt tcaggatata agtttgaggt taaggaatga atagtttaag
     3541 aaagattata gaaaaactga aaaatgtaga tatttcaaat tggattaaac aaaattttgg
     3601 actatattca gttagaaaaa aagtgttttt actttctaaa ttatcaggag cgttaattat
     3661 attattttat ctggttgttg aagaattttc gataaactct aaatttgaat tttggatttg
     3721 gtttataata ctggttatat ttattatgag tattgatttt ttattgggaa aatttatttc
     3781 agaaccaatc acatctataa ataaatctgc aaaaagtatg tcccaacttg atttttctaa
     3841 tccatgtact gtaaatacta atgatgaatt tggagaactt tctaggagcc tgaatacaat
     3901 gtctacaaat ctacaacagg cattatcaga cctagaaagt gcaaatattc aacttgaaaa
     3961 ggatgtaaat aaggaaagaa tgctattgga acaaagaaag gagttggtag atgcaatttc
     4021 acatgaaatg aagactccac ttggcattat acgtgcgtat acagaggggc ttatggatga
     4081 agttgatgaa gaaaaaaaga aaaattatat gaatgttata atagaagaaa ctgatagaat
     4141 gaacaatatg attgtatcat tattagactt atctgcattg gaagcaggag tttctaaact
     4201 taacccagag cgttttgatt ttatagagtt tgttgaaact gtggcaggtc gtcttttaat
     4261 tgatatacca gatgccaatt tttattttac atatgatttg ccagagtatg aagtttttgt
     4321 agtggcagat aaaatgagaa tggaacaagt agtagagaat ctaattatta atgcaaagaa
     4381 gcatgttgta tataatggta atttagattt atcgattaca tgtgaaaatg gcttgctttt
     4441 atttgaaatt tataatgatg gtaggattat agagcaagat gagatttcaa agatttggtc
     4501 taagttttat cgtagtgttc aatctcagcg taaaggaggt tcaggtctag ggcttgcaat
     4561 tgtttcacaa atacttacaa tgcaagggtt agaatatgga gtgaataatc gtgatagagg
     4621 agtagagttt tattttatga ttccaattga tgaataattt agtattaatt tataaattat
     4681 aagtatctga tgtaaaatat ttttctttgt atacgtaagt atttaaagag aataaaataa
     4741 aaaacttaat gaaaaagtct aataaaaagt ttaatgaaaa gtcttgtttt cctgatgaaa
     4801 acaagatttt ttattaagct ttttattatt tcacttcgat ttcacaagag agacactctg
     4861 atttcaccaa atagaaataa aataggttaa ataaattaaa aaatagaagc gtaattaaat
     4921 aaggttacaa gggggagtac ttatgaaatg gtatacatgg ttgactatta tattgataat
     4981 attgctttct attccgctta aaattaaatt tataaaatgg tgggatagac gacaaaaaaa
     5041 tgataaagga ggttatgata gatgataaaa gtaggagagt taaaattttc ttatggaaaa
     5101 gataaacaga tattgcatgg acttaatttt gaagtaaaag aaggagaaat ctttggattc
     5161 cttggaccta atggttcagg aaaatccaca acacagaaaa tcttaaatgg cgttttaaaa
     5221 ggttacaatg ggcacgtatc cctttttggg aaggaagtta aagcatatac agagtctcta
     5281 tatcaaaaga ttggtgtttt atttgaattt ccttatttat ataccaattt aagtgcaatt
     5341 gataatttag aatatttttc gtctttttat cctaaaaatc aaaggagaga catatatgaa
     5401 ctcttagatt tgttagagtt taagaaagag tttataaata agcctgtatc atcctactca
     5461 aaaggtatga agcaacgtat tagcatggca agagcattgg taagtaatcc aaagttgtta
     5521 tttttagatg agcctactag tggacttgac ccatctggag ctgtactatt tcgtaagatt
     5581 attgaagaag aacgaaaaaa aggaacaact atctttttga ctactcataa tatgctagat
     5641 gcagatttaa tgtgtaacaa tgtggctttt attgcagatg gcaagattat ggtaatagat
     5701 aaacccaaaa atttaaaaat gaaaaatagc aataataaag ttgaagtgga atttgtatat
     5761 aatggaaatc gagatattga aattgtagat atacaagagt tagaatctgg aataactttt
     5821 aaatatgatg aaatcttaag tgtacattca aaagaaccta cactagaaga agtttttatt
     5881 aaatacacag gaaggatgtt agtgtaatgt attctaaatt ttttacctta gtaaaaaaag
     5941 atttttggat gatgatttat ggtaaatttt ttgtgctgac acttggctct ctcatacttt
     6001 attcgtgtta tattaatttt ggttatgtaa agtttatgga tgaagatagt tatcacaata
     6061 atgtatatct atataatcca gagaatataa gcatgagtgc atctccatta attaatcaag
     6121 tttcttctat aggtgaatta aaagcaaaac ttgatgaaga tacaagtgca gtttgtattg
     6181 atatgagttc cagtaagccg aaagtaattt tacatgaaag tattaaagga gttgacaatt
     6241 atagagctga ctatgcattg tcattattat catcagaaaa agaccataat agcaaaatat
     6301 cagacaacaa tgcaataatt caaattaatg ataaggatac atctgagata aaacaaagaa
     6361 aagacatgac ttgtgagatt ttattctttg aaattgtagc tattggattt ttagggattg
     6421 cctctttatt atttaaagaa aagcagatgg gagtaatgcg tattactggt attttacctg
     6481 taaaaagaag tctttttata ttatcaaaga tatttgcttt tttgatatgt gacctatgtt
     6541 ttacagttct tttagtagta tttaatattg gattttttga aggagtaaaa atacttccac
     6601 aagtattagt tcaagctagt attttatcaa ttattatgtc tttgattgga ataggatgct
     6661 caatgttatt gagagatttt aagcaatttt ctcttatata tcttgtggtt gctgtattca
     6721 taactacacc tatatttcta tctgcaaata catcttttga aatagattgg attaaatatc
     6781 atcccttcta tcatgtttat agtcagctta aaaatgcgtt tttcatgaat ttttcaaaag
     6841 aattagttta ttttgtagtg tgttctttga caataatagt tctattttta cttgtaaaat
     6901 atatttttaa taaagagttg gttagggagg gataatcttg ataggattac gttaccagtt
     6961 aaagagtata aaaaaagata aaatgtgtat aatttccttc tttttaccag ttgttatggc
     7021 gattttaata aattttatgg gaacaattga tatatcttca ttaggagaga tacgttttgg
     7081 tatagtgaag gatacacttc ctgtacaaac agagcagtgg ttgaagaagt atggtatggt
     7141 aagtgtatat gacacaaaaa tgcatttaat acgtgaaatt aaggatatta aaacggacac
     7201 aattggagtt gttagagatg gtaaaggaat aaaaactatg ctttatggaa atgagattaa
     7261 tgctataaaa aatgttggaa agacattgcc agagatgttt agactaaagg gtgatttaaa
     7321 agatgttgag gttacaatat tgcctaaaga tagtatttta aaagagtttc agaatgtttt
     7381 tattgctatg attttaatta ctgctatgtt tatgggatgt acctttaatg caatgaatat
     7441 tatatctgaa aaagaagatg gaatatcatt tataaatgaa gttttgccac agagtagaag
     7501 tcagtatatt atacaaaaag tatttttggg tcttttatgt gggtgtttat cttcaatgat
     7561 aactgtattt atatgtttgg atatttcaat attagatatg ataatgatgt tgttactcgt
     7621 tgtattttca gcatttattt catcacttat aggtttattt attggaaagt tgtcagaagg
     7681 tcttatggta ggaattgttt atattaaaat agtaatgatt ttatttatag gtgctccttt
     7741 gttaatatat cttctagggg ttaataccag cggtataggg aatttatgct atataatgcc
     7801 atcagttgct acatttaatg gtattatgga gattattaat ggtaatgtta ttattataaa
     7861 agatattgta attttggttg tacattgtgt agtatggttt atgatatata ttattttaga
     7921 taaaaaaaga aagttatctt ttagtagata actcagtata attataattg ggacaattta
     7981 ataggtgatt taatatactt tgtatagtgt aatttatgta gtgcaatttg tatactttaa
     8041 tttatgtagt gtaatagtta gttttatttt ataattattc aatataattt agtaagaaat
     8101 tcagtacaat tagagctact tagtggaaaa cttaataggt acttaaacta tataaatact
     8161 ttattttcct ccatttgtac tataaattta atttatgata taaatggagg tattattata
     8221 tgaggtatta ttatataaag ttaataaaga tagtatcaaa atattatttg ctataaagag
     8281 agggactcaa aataaagata aacctatttt tcaatgttga aatgataaaa tatatttgat
     8341 ttcatataaa aaattttctt cataaattaa aaagtccatt ttaagccccc tcatttttaa
     8401 ccagataaat cgagttaaac tatttaaata tattactcgg attcatttga cttagatatt
     8461 ccttgcttgt aaaattgttt tgaatagggg atagccaaag caaaaaataa aagtataatc
     8521 cctgctacga ttattaaatt attaatagaa attacatctg ccattggtcc gaaaatcatc
     8581 ataccaagag gaataaaacc agtatatata ataataggta agctaagaac tcgacctagc
     8641 atttgtggtg ctacatgctc ttgcaacata gtcatagctg atgcttgaat aattggaatt
     8701 gaaaaattcg taaaaaacat aaatataaaa aattgccaag cttgtgttac aagacctaga
     8761 ataattgaaa aaatagcata ggataatatt gctagaaaaa aggtcttatt tctattttta
     8821 aaaccaccaa aaactcctat aatcaatcca cctaaaacca taactaaaaa tccaatcatt
     8881 tcaataatag acaggtatat ataacccttg ccaaatgttc gttcaataag aagtcctata
     8941 agaaatcctg atggaacaca aagaagcatg aatgctccat aagtgaaaag cacttttcta
     9001 atgaacttat ctgaaaaaca gtactttatc cctgatacca tatctgcaaa aaatgagact
     9061 ttttcagttt gttttggtat attatgttta ggaattttga tgaatatcaa taatacaata
     9121 gcaataattg cagtaaatac atcaattaaa agaacttgat ttattttatt accaaatcca
     9181 agtattgcag cagctacaat aggcactata aattgtacta tagactgtag actactattt
     9241 ataccattaa aacgcattaa atgttcttct ggtacaagtt gaggaatcat tgcgtttaca
     9301 gcaggtgttt gtacaccact tcctagtgaa cgtataacag atattactga tatggctatt
     9361 aattcacttg ttgtgccatt tgatgacatc ataaatagaa ttagaactaa tgtagatata
     9421 gcaattgttg aatctgatac aattatgagt acctttcgat tgtagtagtc tgcccataca
     9481 ccagcaaaaa aagaaattat catttgaggt ataaaggagc atacagttaa aactgttacc
     9541 catacacctg atgatgtctg aagtgtgaca taccatatca ttgccatttg tacaagtgat
     9601 gagccaaata aggaaatata ttgacttgct aaaaaaagaa tgactttttt cttccatgta
     9661 gaattagtat ttttgttttc catcctattc ctcctttgat ttgtgtgttg aatttttctt
     9721 gctcttaagg ataacacaca aatcaaagtc tggagttaga gtggagggct aggaaaattg
     9781 ttttttcata atttgaagat gagatttcat ggtttttgca ttatgttcag cttctttcaa
     9841 tgaagttatc agtttgtcac acactgagat aatttcgtca tcatcttttg gagtgtcaag
     9901 gacagtttta atattagtat taggattaga agataaagca tttaacattc gtaaaatagc
     9961 tgaaagagaa tagtttgcac aacgaagtgc gcttataatt ttaagtcgtt ttatatcttc
    10021 atcagtatat acacgataac cattttgttt tctttttact tttaacaaac cattcatttc
    10081 ccaatttctt aaagtatcta ttgtaacatt taagtgttct gcagtttctt ttctagttaa
    10141 aaagagttta tttgtttctg gagtatttcc atttaaaatc tgtgcaacaa tagtaagtgc
    10201 ttcctcggca ttagattttt ctatttctaa ttgttgcaag tattgattta caagactaat
    10261 agccttatca aagtccctat tcgctgatgt tcttacaatc tcaagggcat cttttcttaa
    10321 tcccctttgt accacttcaa ttttcaaggc tgtacgagca aatctaaatt gttctatatg
    10381 aaaatcatta aagatacgat aaccattggt attacgctta ggtttaggta ttaagtttag
    10441 ttcttcatat aaacgtactg tattaggatg tacaccaatt aaatctgcta cctctgatgt
    10501 cgtgtaagtg tccattagta ttcaactcct tttagatata tagtattagt cataataaca
    10561 ccaaacaaaa gtctggtgtt atagtggagg gtttggttat ctaagagtaa ttagtttatc
    10621 taagagtgat taatctatta tagaagtaag tatatatttt tcaataaact cccatctatc
    10681 acgaggttta aacactattt tagatggtat ggctattact aagtcatatt gtggaataca
    10741 acaaatagca tttcctccag ttcccattgc tgaataccac aaaacaccat cttttttaca
    10801 tatccaccac agataaccgt attcattact attcatttta accgattctt caatccacga
    10861 tttagatata atacgtttat tattccaaat accatcattt aaatacagaa aaccaaatcg
    10921 caacatatct ctagcactca atgtaagtcc ccagccacct atagaataac cctgtctatc
    10981 ttttgcccaa cccttaagat tttttccaaa gaaatcttca aaaccaaaat ctttcatatc
    11041 ataatctggt ataactttca taccaatagg tttaaataag tactcattag caaattcacg
    11101 agcagatttt ccagtagttt ttgttataat agctgaaagt aggtgcgttc cagctgaaga
    11161 atatttgaaa gtaccaattt cgcctttttg accaagttga ttcaaggtat attttatcca
    11221 atctttttgt ttgcaaagtc tttctaaaga ctcagtcata tttttaaaag ggtatggtgc
    11281 agtcattgta agtaggtgac gtatagttac tgctttttta catatttcag aaggactaca
    11341 agtatattca gggaaaaaat caacaacctt ttggtcaaca tcttttatat atcctttatc
    11401 tattgcaata cctattaagg ctgatataat actttttgta actgatgcaa catgaaaagt
    11461 atcatcagca ttatatccat tatagtactt ttcaaagaca acttttcctt ttcttgcaat
    11521 aattattcca tttatattac tatattgtga gattattaaa ggttccagtt gtgagagctt
    11581 ttctgaactc atgccaacac ttgaagggtc tactttttca aatattaaat ctaagcaatt
    11641 gtttttattc attaaagata cctccctgtt atagattttg gattttcttt tagagttttt
    11701 ggagaacctt ctgcaattat ataacctcct ttgtcaccac cttctggacc aagctcaaca
    11761 atccagtcac aaacatttaa tacatctata tcatgttcaa caacaattac agaatttcca
    11821 ttttccacca attcatctaa taattgaatc aacttagaag tatcatatag acttagacca
    11881 gtagttggct catccaatac ataaaggata ttaccatttc gttttttacc aatttctttt
    11941 gcaagtttga ttctttggga ttcaccacca cttaaagtcg gtgtaggttg gcctaatttt
    12001 atatacccca tacctatttg ttctaataca tttaagtgtg aaacaatatt tggaatatcc
    12061 ttgaagaaat gtacagattc agaaacactc atatttaaaa tatcatgtat atttttattt
    12121 ttatatttaa tagataaaga ttcatcatta aatctttttc cctgacattc actgcatata
    12181 tggtctatac ttagattatt tcctaaccag accttttcat atccactacc actacattta
    12241 ggacaagcac ctttggagtt aaatgagaaa tgaccagctg taaatcctcg ttctaacgat
    12301 tctggctgac ctgcaaatag tgtacgtatc ttatcccata taccaatata agaagcagga
    12361 gttgagttca tatttttgcc aataggagat tgagatatct ctgaatatcc agaaatatgt
    12421 ttagtaccaa tcagtctatc tgcaatagtt tcaataactg cataatcgtc ttcatcacct
    12481 atttcgctat ctgaagaatt tatttgatta ttgatagtat ggttattaaa ttggcttcta
    12541 agaagaggaa gtagggtgtc tgatataagt gaactcttgc cactacctga ttttccagat
    12601 attccaacca tagcacccaa aggtaatgat acagttacat ttttaagatt atttgtattt
    12661 gcattttgaa tagttataca aggtatttta tcagtatgtg taatattttt attgcttgtt
    12721 ctattaggca ttggatattt tccagataga tactgcccag taagtgattt gtcggattga
    12781 agtagacctt ctaaatctcc ttgatatact aattgaccac cttcaacacc agcttttgga
    12841 ccaatatcaa taatatgttc tgccatttca attgtgtttt tatcgtgctc aactactatg
    12901 accgtattgc ctaaatcttt aagtttcttt attgatttta agatgtctat tttttctgat
    12961 tcatgcaatc cagctgttgg ttcatcaagc acataaatta atgagtccat ctctgaatcg
    13021 agatgtgaat ttaaaaatag tctttgcatt tccccaccac tcaaagttga catttctcgg
    13081 tataaggata gatgacctaa tcgtgattct attaaatgtc gagttttgga taaaatatca
    13141 tttacaagat ttgtaccaaa tgtgtttaaa ttaccttggt ttattaagat tttaaggaaa
    13201 tcatcaattt ctaaaatcgt catttttcca agttctccaa tatgttttcc gtttaaaagt
    13261 actctacgtg cttcttcacc aattttaaaa ccattgcaat ctgaacaggc agtcatagta
    13321 taaactccat ttaaatcctc accccttttg taacgattgt gaaatacctt tgataaagag
    13381 gaactttttt taccattttc ataatgacca tagagtactt catttcttat ttcttcaggt
    13441 agttgtaaga attgtatgtc taaataatcg ttgaattttt tagaaaatac tctcaaatat
    13501 cctggtgtaa tctttgctct atctaagact tgttttaggg tatcatgctt atcaaggact
    13561 aatttttcaa tattaatctc aaaatatgca ccatgtccag agcacttcat acacattcca
    13621 tctgaagagt tgtatgagaa ataacttggt cttaatcgtt cttcctcata cccacaatta
    13681 ttgcaaatta gattatcgct tacaggagta tcacacatag tacacattat ttgaccttct
    13741 actgaataca atagtgcaag catgttgaga atattggttc ttgtgcctac tgttgaacga
    13801 ggattgctct ggcgaataat attttgttga acagctatag tgggagctaa accttgaata
    13861 ttatcaaact tatcctcact atcaatacct gcaagtatac ctaaagattg cagatattgt
    13921 ttacgacctt cctcaaatac aatgtcaaac ataaggctag acttacctga gccactgaca
    13981 cctgttgcaa caacaagctt atctttgggg atagaaacat ctatattttt aagattgtgt
    14041 atctttgctc cattaatttt tatttctttc attggttcac ctcattttat caattttatc
    14101 aaaccgtaaa atagggatat attaatcctt aatacaagaa tagattaggt gaggttatta
    14161 aaaaggttct ataggtaaat atatatcgac aaagtgtcta ttttgagggt gattgtgtgg
    14221 gttgttaaga tatacttcaa aagggaagga ggcacgtagc ttataattac catttgatag
    14281 ccattcatta tacataaaat cccacgcatc actatattca gattttgaaa tatcaaaatg
    14341 tcccacaaga tattttccag aagggattat catattgcca agattgtcat tctctttgac
    14401 aacaatgtta tctggaatgg tcatgcaaat actagtcctt aattgatgct cctgtgtaaa
    14461 ttgtggatta tcatgataaa tggctaatat ttgtgtgttt tcaagtccaa gtagattctg
    14521 ctcacttgca tatgtgaata accgttctaa aagttttggg aaagtactgg ttagattacc
    14581 atatgaacca gtatatctag tgtaaatagt attcatatta tcaagttcta gaatttcaat
    14641 ttcaccttta acctctctag tattcttaca tttaggtttt gatatgcttc tattgtactg
    14701 agaaatttta tatggttctt tgcaattctt gctataattg tttctatatt ttatagggct
    14761 tattttatag taatttttaa atgctctcga gaaaactgct gaatcagtaa agccgaagta
    14821 gtgtgcaata tcagtaacag tcatgtcagt tcgatgaata agaaaagcag ttgcattttc
    14881 tagttttagc ctatttacat attgtgccaa agtttctttt tctattgctt tgaagattct
    14941 atgaaaatga aattttgaaa aacctgctat attagaaagt tcatcaagag aaagaggttc
    15001 atgtaaatgt tcttctatat agtcttgcac tttgcaaata cgatttagat attctttttt
    15061 acttttagca tttgtcatat atagccctcc ttattgtgta aaatgtgtat taattaattt
    15121 atagcaatat agtaataaaa ttttatgttt ttgcaattta tgataaataa gatagcgttt
    15181 aattttttat aattgataaa agatatttat aattaataaa agatataaga tgccttatat
    15241 ttatttttgc atataagtat agtcttcgta ataattagta taccatagtg atttattttt
    15301 aattgtgtat ttatataaat acacaactta tgatatggac gaattattat gcaaataagc
    15361 tgtaattaaa taaattgcaa tataaaccag ttgaaacata agttagggaa atattatcaa
    15421 agttattatt tagcataaga gtatagaggg aataaaataa gattaaaaaa ttacatacta
    15481 aatttaataa aattattata ttaaaaataa tattaagttt attgtttaaa taaaaaagtt
    15541 aaatctacac tattaactta ttatacttaa aactttaaaa tgaaaatatc aaagaatact
    15601 ttttaataaa aataatcatt taataagtta tttaaaaatc taaaaacttt acaacaaatt
    15661 atctatttaa attggaggag tgggaatatg agtaatagtt tttatcaatt aaattcagca
    15721 gtattatatt ttaatgaaaa aagagatgta aatagaataa ggtttaatca ctatgtaatg
    15781 agaaaagaaa atatacaaag attaaaagga actctgccaa gaggacaatt tatgatgaca
    15841 gtaaggaaag ctgcatctga ccttgatgta tctatatcta caataagtag acttgtaaat
    15901 gaatttatag atttaggtat attgagatta ataagtaggg gagtaaaggg aaattgttgt
    15961 tctgtatata gttatgtttg ttctgaaaat agtaatgcag aaattgtaac aaagaatagt
    16021 gatattttca aaaatataga gaaatacatt gatgcaacta ttaacaataa ccaaaaaaca
    16081 atgaaatata aacatagaga tgagttgttc aagaacagta ataccaacac atacagccgt
    16141 ataaatcaag agagtataga caaagattat gatttgataa ttcatactgg gtatgaaaca
    16201 gaaaatgaga caaagaaaaa agaattatta aaaaataatt taaataaaaa gaatttaaat
    16261 aaaaatacct ttgaaaattt ggatgaagat ttgactgaag attctactta taaaaatcca
    16321 gaaagcatac atgaaattat tataagaaaa ttaaatagtg aatcaggaaa aaattttaaa
    16381 gctgatttga gtataactaa ggcacttata gataaaagat taaaagaagg gtatagttta
    16441 gaagattttt acaaggttat agaggcaaag gtaagaagat ggagatatac agatatggaa
    16501 atatatatag aaccaacaat tctgtttggg tatgaatttg agtcgtattt aaatgaggag
    16561 agttctttat ctgcttgtaa ttatggtgat atgtgttatg aagatatgta ttgagatatt
    16621 agttgctttt tagttgttta aaatatgttt atatataaat ataaatagtg tattaaaatg
    16681 gagttgtctc aaaatagaga gaagcctctt cttaattgtt taaatgatga aaatatattt
    16741 gatttaatac aaaggagagt gccttatgag ctagaaagtt cattttgaga cactcacttt
    16801 tttaatatac tggagaaata taacccattt taatggcttt tgtaatagcc tcaactgatg
    16861 atgaaacatc tagcttgtca tatatgtgtt gtaaatgatt tgataatgtt ctttcactta
    16921 tatacaattc atcagcaatt atttttcttt ttttaccact acagataagc tgtaaaattc
    16981 ttttctccat attagttaaa tcttcaataa ttacttcagt attttcagat ggaaaacagg
    17041 ttaccccttg atagactttt aataatgaat caattaaact atcagggcta atattcttat
    17101 ttaggaatcc atagatacct atttttttag cctcatgttt ataaactggg aggtcgtatc
    17161 cagtcaagat tattatttta atagttgaat tagtctctaa taaattataa gctactttta
    17221 agccatcaca ttcacttaag cctctaaggt ttatatccat taagacaatg tctatttttt
    17281 tatcttgaat catttctgaa agattatgga tgtcctgtgt ggaataaaac tgttcgattt
    17341 cttcgtattc ttctagtgca attgacaagc ttttagcaaa aagcatatgg tcatcaacta
    17401 acaaaatatt tataagaacc atctcctttc atagggattg taatctgtac acaaactcca
    17461 tgaggtatat tatcagaaat acttatcgtt cctcctagat tttctacttg ttctttaatt
    17521 gatataatcc ctttgtgttt tattatatca aaagtaattt tagttatatc taaaggtgtt
    17581 ttaaaagatg tatttttcac accattatca ttaatattta attttattat atttttttct
    17641 agtgctaaag ctatccatgc atggctacca tttgaatgtt tatacacgtt tgtcaataat
    17701 tctttaatca gtcgatatat caatatatca tatggttcaa ctaagaataa agtatcagaa
    17761 cacttaaatg aaacacaaat gtttttttgt ggaaaggatg aagaaacact ttcaattaaa
    17821 ttctgataat tttcttttac tgttaaattt tttaaaataa caggatgata gtcttgcatt
    17881 tgatttctta tacatgtatt tagattgtta agtgtttcat atataatatc ttgaacttct
    17941 gggcgatatg acttactcat catgtttttt attgaaagca ggttctgtaa tatgtcatca
    18001 tgtaaaaaat tagaaaactc tgtttttagt tgttcttctt gttgtaattt ttctaatgca
    18061 aaagtatact tgctattttt aactacttta tttttttcat ctctaaggtt aaactccaaa
    18121 ataatgttac agaggaaaat aagcataaat aaaacattta tagctattaa tattatagaa
    18181 aaattcccat tggcaaatag tacgaccaag ctaattacta aaatacttat aaaaagaaat
    18241 atagcccttt gttttatgtt aaaaactgtt gaaataggaa aactatcatt ttctttaagt
    18301 gcaataccat gaatactcat aaaaaatacc aatacagtta gatagattcc aaaattacca
    18361 atatgatttg atattcttag tgtaaaaaaa taataagtaa ggaatcctat aattgttata
    18421 aatactgaaa tcgatatagg ctttttttcg ctttttatga caaaaacaac ccttttcaaa
    18481 tgataaaaaa taatatataa aaagcttatt atactaacaa caaactgtat tccgtacata
    18541 caagcatacg ttctgttaga aataaatgct cctacaattg caccaatgca ggtgataagc
    18601 aataaaatat ctgttatctt acggaaattg tatccactac cttgaaacaa aaataaaaat
    18661 ataaatttaa tagagacata cactataact gtgcttaata ttttaaaaat aatagtgcct
    18721 attggtgtgg ttacaaatga taagagtaaa taccaactgt ctagtgctag taatccacaa
    18781 aataatgaaa taatgttatt ttctcggaca ttacaagagg ttatatatgt gatgtctatg
    18841 agtaataatg ttgaaattat taagggaata atgctttgga tattcatata agtttcagtg
    18901 cttgtagata ttcccatgaa gagaagtgat agaatgtagt ttcctaaaaa tagtattttt
    18961 aaattaaaat ttgtcaatct tatacacctc cttatgagtt aattatatta attttatgac
    19021 taaatcaaac ctaagaatct ataattttaa attatatcaa atacttataa atgaagtata
    19081 ccaaatcgtc acacctctaa gcttgacgat ttggtaaata ttaatttttt aatacaattt
    19141 tacaacagga tttaatagta agtatataat atatcaaata cactacagaa gttaagaaaa
    19201 ttgcaataat cacaggtatg tattgtgaaa atgaatttcc aagtaaattc tgcatcaaag
    19261 cagtcttata aaccaaggta gcaaatatac agtccaatat accaagtaaa aacggaatat
    19321 taaaaaatgg caatacttgt tttttaataa tttttttaat ttgtatatta tcataaccca
    19381 ttttctgtaa aatcttataa tcagatgttg aatctgaaat tgctgatata ttgttaaagt
    19441 ataaaatact tccaacagca ataaagaata ataccacaag aaatccgatt aacagaaatg
    19501 tagaactatt aattgataat aaatcattta tcctgtgaga atatccttgt aaataagggc
    19561 ttccatctaa ataatctgaa atattactat ataaattttc attattttta attgatttac
    19621 cattaatact taaaatgctt gtcattggta atttattttg tgctatttgc tcatatatac
    19681 tatcattaac gataagtgtt ccaacgctat tggcaaaaga aatagcattg tttaatgatg
    19741 tttcccttac agctacaggt gtttctttac cattaataag taatggataa atcgttccat
    19801 tttcactata tccatcatta tttggttgat atttcattaa aatacattca ttatccctta
    19861 aaggggtaag ttgttttagt agctttgttc gattttgtgc ctttaataaa gtataataat
    19921 cagaaaaaga tatacattca aatcctgatt ctcttgaaat aacttcttta gaagaactac
    19981 tattagataa attattctta gatgtaccaa tttcatattc aaatggaagg tcttttgatg
    20041 aagattttac cttataaata tttgtctgtg taaaaataat atcatctgta ttagacgtat
    20101 attgactgac aatttgtttt acatcttcta tttgtttatt tttttctact ctaaattcta
    20161 tctctgaagg tgcaatacgc gaaacagatg caatagggta gtataaggac agtgccataa
    20221 cacttgaaac tgtcaaagta gtagcagaga gtaatgtaag cataattaga gttttagagt
    20281 ttgaacgtat acgatagata aagtttggaa tagctattat cttcatatct gtgtagaaag
    20341 tacgtttatt ttttttactc ttttgcataa tatatggaag aaaggaattg ataaagagaa
    20401 ctgtaccaat tacaatgata atcatagtta tcaagcccat ttgataaaat ccaatactta
    20461 cccatactga tttagctcca cgaagaatat ctagtgataa acaataccca cttaaaatca
    20521 taataaatcc cataaaggct ggaatttttc tgaactttaa ttttttttct gcactttttt
    20581 catgtcttat caattttaca agtgaagtct tataaagaaa tttaccattt gatatagcta
    20641 agactaaaac aactaatgct ataaagataa ttgttttgtt tattgcattt atattaaaaa
    20701 atggtatctg tgaattatca attgataaat taagtagtgc tgtaattcca aagacaatac
    20761 ccttatgtga caatgctcct aataccaatc cagtaataaa agctccacta caaattaata
    20821 cattttcaaa ggtaagtagc gataatattg taaacttacg atagccaagt aaagcatata
    20881 tgcctaattc ttttgtacga cgtctcaaga agaatcgatt tgagtatgcc atatagaaga
    20941 tgacaaatgc catcaaaaat atcgaaataa cattacacat agattcaact ctaccatcag
    21001 ttgaaatttt ttctagcatt atcttattaa cagagaatga agtaaatgcg aaaaatacag
    21061 taataacaaa agagactgaa agtagatata gagaatagaa tgaaaagttt tttttaagat
    21121 tctttattgc catataaaaa atgttcatta aattatcctc ctatctttta gtatccaatt
    21181 ttgcaatttc ttgaataatc aactcataaa atgtatgtct attattcggt tgtttattta
    21241 attctttaat gatatgacca tctttaaaaa tcaaaattct atttgcaaaa cttgctgcat
    21301 aggcatcatg tgttaccatt aaaattgttg ttttcaactc ctcatttgct tcctgtagtg
    21361 agtggagtag ttcagtagct gaattagaat ccaaagctcc tgttggttca tctgcaaaga
    21421 gaatactagg atgttttact attgctctaa ctgctgaagc tctctgcctt tgcccaccag
    21481 ataattcatt tgggtattta tttaattgag aagctatgtc aaatcgttca gctagactct
    21541 ttaccatgtt ttctatttta tctggattga gtttctgaag tgtaagaggt actgaaatat
    21601 tctcgaaaat tgtaagacta tctagtaaaa aatactcttg aaagataaag cctaaattat
    21661 ccttacgaaa atcagcagca caagtgtcat taagcgattt caaatttata ttatttatga
    21721 aaatatctcc acttgttggt ttatcaattg tagataatac attaagcaga gttgttttcc
    21781 cagagccaga agctcccatt atacctataa attcaccttc tgatacatta aaggatacct
    21841 tatctaaact cggctgttgc cctttattat aagttttagt tatgttattt acttttaata
    21901 ttgtattcat tgtatatgtc tcctttcgtc ttaatatagt caaattatat attaacctta
    21961 ttgtttcttc atgagtaaat cacgcaataa ttctacacaa attacttata tcacaatata
    22021 ttgtttaaaa tctaacttta ttaggtttgg gatgatggac aagattttaa tttaaaagag
    22081 tgcatagata tataatggca taatgtagat ttaaattaaa aaaaataaaa taaagccata
    22141 aatattgaaa aattcaatat ttatggcttt attgcttgta ataattaata gaatatatat
    22201 tttttattaa tcatctattt caaattctaa tattttacca gtaattgcat taattgttat
    22261 ctcatattca atttttcctt ttattatttc accttcatat tctttgtcat cttcatcata
    22321 cttaaatttt actattttcc caccaggcac tttatctagc attattgatt tagctttttc
    22381 agcactaata atagaactgt tgtcattagt attagagtta ttcttatcat tatcatcaaa
    22441 gtcatcatca gaatcattgt ccatgtcaat atctacaatt cttccatcaa atgcacttat
    22501 ctctaattca tatgttttct tattttttat tactttagcc tcatattcgt tatcttcagc
    22561 atcatattta caatagatta aattaccatt aggaacagca tctaatgcta tagacttagc
    22621 tttatccaat gatatatttt gtgtacctaa attattatca tttaagtcat catctttttc
    22681 aatttctatt attttaccag agtaggcatc aatttctatc tcatatagga tattatcctt
    22741 tataactttg cagtcatatt cattatcatc ttcatcataa taacaaacta aaatatcgct
    22801 tcctggaaga gcttgtttta atatttcttt agcctgtatt aaagatattt ttttagtttt
    22861 atttcttatg ttcacatttt ttagttttaa aatatcttca aatgcttttt gattatatcc
    22921 tcctcctact tgtgttattt catctatatc tttattttta ataaattcct tttgactatt
    22981 tacaaggcta ccattagata tcattatagg agaattttct ttagcagcta atactcctat
    23041 tgataatgaa tctactaatg cattttcttg gttaatacca tctttagcta caaatacatt
    23101 atttattttt ttatctttat aaaatttatc aataattttt gcattagtat tgtttctatc
    23161 tgaaccactg attctttctc tgtttggata tttctttact gtattgtctg ataaaatctt
    23221 atttcctcca actatgtaag atttttttat agacctattg tttaagaaat ttatactact
    23281 atcattgctg ttttcatctg atactaatat tataggcata ttatgtattg ctgctggaga
    23341 agctatacta actgcatcag ataatccctt tagtccattt acaactgcaa cattttctac
    23401 cttttttatc ttatctattt ctttaagtac atttgtagac attttatata tattgtttcc
    23461 tactatttta tttattttta tgttaagtga ttttatttca ttttctactt tagaagaaat
    23521 gctatttcca ctatctacaa tgtacacatt atctacacca agccttttaa gctcagcctt
    23581 agttgaattt tcaatactgt tgttgtttac taaaagtata ggtgcattct ttagcttagc
    23641 aaatggattt gcacaaagcg cactttgaat agaattacca ttaactataa tggctgtttt
    23701 tgattgaacc catcccaatt tacttacttc tactgctgtt tcatatcttg ttttacctat
    23761 taatctttct tgctttgtag tattgtctgc aaaaattgct gaagaactag aaaaaaacat
    23821 catacctgat attaatatag ctaaattttt attattcata tttttacctc acaatcttta
    23881 aaaatttgtt atttagcata cttaataaaa tataatctta aaatcaaatt gatactgagt
    23941 ttgaagttac aaaatcaaca aataagattt ctttaataca aaattttcct ttttatattt
    24001 aacattgtat cacaaatata aaaaggaaaa aactttttaa aaagggtata cactattcat
    24061 tttttatctt ataaataagt ctttaccact gttttatata ttttaaaata gacacaagac
    24121 ctatttctaa atcttttata aatattttag gtgaaatttc tttttaaaca tatctaaaaa
    24181 gtatttgtag taatattaat ggactgaatt tcttaaagta tatatttctt tgcatgttgg
    24241 agtttgtgaa gtttgagaat tggagggtga aagaaacatg caatggatta atattaaaga
    24301 actgtatttt tataatattt gtcttaaaaa taaaaaaatc tattgacttg aagttaactc
    24361 caagtagtaa agtataatta tacaagaatt atatacttgt aagaatagtt aaagtagaga
    24421 gaaaaaacaa acaagtttta aagggggaag taaaacaatg gaggcaaaaa aattaggatt
    24481 tggattaatg cgtttaccaa taaaagatga aaatgatgtt acaactattg atatggaaca
    24541 tttaaataaa atggtagaca cttttttaga aaggggattt acatattttg atacagccta
    24601 tgtgtatcac atgggaaaaa gtgagatagc acttagagaa tctctagtaa aaagacacaa
    24661 aagagagagt tttactatag ctacaaaact tccattaatg gctttaaaga aaaaagaaga
    24721 gcaagaaacc atttttaatg agcaattaga aaaatgtggt gtagactatt ttgactacta
    24781 tcttttacac aatataggcg tgtcacatta tgaagtagct aaaaaatttg atagctttaa
    24841 gtttattgaa gaaaagaaaa aagaaggaaa aataaagaat attggttttt cttttcatga
    24901 tagtgcagaa ctattggata aagtcttgag tgagcatcca gaagtagact tcgtgcaatt
    24961 acagattaat tatttagatt gggataatga aagcatacaa tctagaaaat gctacgaggt
    25021 ggcagagaag cataataagc ctgtaattgt tatggagcca gtaaaaggag gtacacttgc
    25081 aaagatacca gaaaaagcag agaaattatt gaatgactac aatccagata tgtcaatttc
    25141 atcttgggca atacgttttg cagcaagtaa agataatgtt atgatggtct taagtgggat
    25201 gtcaaatatg gaacaattat tagacaatac aggatatatg caaaatttca aaccattcgt
    25261 acaagaggaa tataatatta tagatgaagc agtagagatt atcaacgaat caattttagt
    25321 tccttgtact gcctgtcagt attgtgtaga aggctgtcca aaaaatatcg ctattccaaa
    25381 ctattttgcg ctgtacaatg cagaaaaaca agctcttaat actggatttt caacacagat
    25441 ggtttactat aataattata ctaaaactta tggaaaggcc tcagattgta ttgaatgtaa
    25501 acaatgtgaa gaaagctgtc ctcaacacat taaaattata gatgctttaa agaatgtagc
    25561 agaaaccttt gagaaataat tcaatctata caatatcaag aggagaagat atgtatgacg
    25621 atttcagaag ttagtaaaaa atacgagctt tcagcggata ctttacgata ttatgaacga
    25681 attggtttaa taccacctgt aaatagaaat aaaagtggaa ttaggtcttt tacagaagaa
    25741 gattgtgagt gggttaattt cataaagtgt atgagaggtg caggtctttc tattgagaca
    25801 ttaattgagt atgtagctat gtttcaacaa gggagcagta ctatcaaagc tagaaaagaa
    25861 cttttaatag agcagagaaa ccaactagca aaaagaatag aagaaatgca aaaaactttg
    25921 gaacgattaa attttaagat tgatagatat gaagaaggta ttattgagaa agaaaaagta
    25981 ttaaagagca gtagaaataa gaaaatggag gcagtatctt atgagtaaaa atgtattaat
    26041 agtatcagct agtcctagaa aaggtggaaa ttctgactta ttgtgtgacc agtttataca
    26101 aggggtatta gaatctaaaa atcaagtgga gaagatattt ttaaaagata aagatattaa
    26161 ctattgtact ggatgtggtg cttgttatga aaaagaagca agttgttcac agacagatga
    26221 catgggagaa attcttgaaa aaatgattgg ggcagatgtg attgttttag cgactcccgt
    26281 ttatttttat actatgaatg cgcaaatgaa aactatgatt gatagaactt gctctagata
    26341 tactgaaatt tgtgataaag atttctattt tatcgttgca gcagcagatg aaaatgtggc
    26401 tgatatggaa agaacaatag aaggatttag aggatttaca tcatgtctaa atggttctaa
    26461 agaaaaaggt atagtatatg gtgtaggtgc ttggaatgta ggtgatatta agggaagtaa
    26521 ggcaatgaaa caggcatatg aaatgggtaa gttggcttag tatgtttgta taaaattgta
    26581 taaaatcgaa tatattctta tcatttcaac aataaaaaga ggcttatcta tattttgaga
    26641 cagcctcttt gtgctatatc atttattttt attctatatc cttattatac cctttatttt
    26701 tttatgccta tttaagtatc tgttgttact catatatttt aataattacc cttaaaaaac
    26761 tatacagatt cctgcataat attattttct ttagtaatta atttttctag gtttttccaa
    26821 tccatttctt ccatatttgc aaaataatga ccacttccta tgcaagtcag ataagacttt
    26881 ccatctaaat caaacgaaga acctccaata cactttgaat tacatttctt acaagtacga
    26941 gttttagtta tactatcttt aatagtttta gagcattcct ccacagcatc tttatatgta
    27001 tttagatgaa aaagatttgc tttaactctc catttttttt cattacattc aatagtaaat
    27061 aagctacgtt ttggtttctt agttgaatat actattttgt aagaacgagc acctgttttt
    27121 ggttcaattt tacaatctaa tgatttacca agttcatgta aatgacatgc aatatcataa
    27181 tctttaccac ttagtacttc taacatttct tctgttgttt ctgccgttaa ttcacttttg
    27241 ctcatattaa aaaaatcccc ctatataagt aataatttca catgaaaata attaatataa
    27301 gtatattaaa taaaactatt aaaatcaata aaattactac aatcttacat tattgggcat
    27361 agaatgagag ctaatgattt attagaaata agacttaata gtatgaaaat ttacttagac
    27421 gtggtacaat tctataaaag atttaaaact aggaaaggag aaagttgttg agatacggcc
    27481 aatataacaa tttatttata agttgtaaat ttattatgag attaattaga ttgttaagta
    27541 atactatcaa aaggtagttt tttggatttt cagcaacagt ggtagatatt gataattttt
    27601 tggttattat tatatttagc tgttatattt tcaataggct ctatgataac tagggtaaat
    27661 aaggtatggg taaaggtttt attatttata atagtgggat tgtcaactat agttctttca
    27721 tatgtaataa cttgataatt taagattgaa aaataacttt aatctgtaaa ttctcaaaac
    27781 aagaattctc atgttggtaa atttctagat atgagaaaaa aattgacatc ataaaaaagt
    27841 aattttaaag cattttttta cgaaaaaatt cagaagtaac tattttgaat gacttcacaa
    27901 aattttatat ataaataaag tttggcacgt taattgcttt aataaaagtt aacaagacac
    27961 aaataaacat aatcatctaa gtattgatta tttgaagttt tagatttgaa atttaatcta
    28021 aaggttcgta ttaatattgg tgtttaggaa attttatttt cctgtaaata taacttaata
    28081 ataggaggtg aagtcatgtc aaaagtagta atagctacag agaaagctcc aggagcaatt
    28141 ggaccatatt ctcaagcaat tcaagttgga aacatagttt ctacatcagg tcaacttcca
    28201 atagatatga agactggcga gttagtatct gacccaaaag aagcaacaaa agcttcttta
    28261 gataatgtaa aagcaatatt agaacaagct ggtacaagta tggataaaat atttaagact
    28321 acagtattcg taaaagattt aaaggatttt aatgatatta atgaagtata tgctacatat
    28381 tttaaagaaa atccaccagc aaggtcatgc gtacaagttg ctcaattacc aaaagatgca
    28441 gttttagaaa ttgaagcaat ggcagtatta taagtattat aacaataaaa ccaataacaa
    28501 aactaataaa aattaaaaac taataaaaaa agtctacaat taaaaaagtt tatattaaaa
    28561 aagtctatat aaaaatctat ctaaatattt aactattgag cttttctcat ataatgtata
    28621 tttttatgtc aaaaatatat ttgttatatg aggaaagctt tttttatttt attattgtat
    28681 tgtgatttac tttattgtct gtatcttata attaagtaat taaggtcatc atataaaaca
    28741 aactttttat aaattaatat tgtgtgtttt aatgtaaata tataaataag taatgtttta
    28801 tatatatttt aaagagtgac tatatgttaa aatatagcta aactgataat ttggtaaatt
    28861 aaattctaat gggggaaact atgttaagta aattaaaaga atttcaacaa gaaatgataa
    28921 aatacactga aacagtagct agtgtgttag atgtagatat tgaaatagtt gatgatagat
    28981 taataagaat tagtggtaca ggtctgtata aatcaaagat aaatgaaagc gttgttacag
    29041 aaggatttat ttacgacaat gttatacaga caggtcagga attagtcgta ttggatatat
    29101 gtgataatca gctttgtata gaatgttccc attacatgaa atgcctaaat aaagtaataa
    29161 tagcagttcc aataaagtat aataatcgaa ctataggagt aattggtgct attagtacag
    29221 ataaaactaa aaaagtagaa ataagtgcaa aaatagataa ttatttaaaa tttgtcaatc
    29281 atatatgcga tttaatttct atgaaaatag aagaacatga agcgtcaaaa aatagcagta
    29341 gaaaaatgga tatgatgata gaaatcattg aaaatgttga aaaaggcgtt ataatactag
    29401 acataaatag caaaatatct tatataaaca atatagctct aaaaaaatta gatatatata
    29461 aaaatataat agaaaatata gtaaatatag tatctgttga atcttcctca aatggacatg
    29521 aattactaga aattgatatt gataacaaaa tttataatat aaatgccaaa ataattccgg
    29581 tatatccata tataaatcaa tacgacaaaa taataatatt tgacaagaca tatatcaatc
    29641 ataaaggaca tggaaaagtc aatagtggct ggggaaatag tgatatagag tctataattg
    29701 gaaattcaga ggcaatgtta aaagtaaaag agagaactaa gaaattagca aagtcaaatt
    29761 caactgtact tataactggg gaaagtggta cgggtaaaga gctaattgct agggctatac
    29821 atgctgaagg tagcagatgg aacaagccat ttattgcgat aaactgtgct gctattccag
    29881 aaaatctcct tgaaagtgag ctatttggct atataaaagg tgcatttagt ggagctagta
    29941 gtggaggtaa agtaggtaaa tttgaacttg caaatgaagg tgttatattt cttgatgaaa
    30001 taggagattt gtcaatgcct ttacaagcaa agttacttag agttttacaa gagagaaaat
    30061 ttgcgagaat tgggtcaaat aaacttatag atttagatat aagagtgatt gctgctacaa
    30121 ataaaaactt attgaaatta gtaaatgaag gtaagtttag ggatgattta tactatagat
    30181 taaatgtaat tcctataaat ttgccacctc ttagggaaag aaaggatgat attgaggcaa
    30241 taatgatgaa gtttgcttct aaatattctt tagaattagg tattcagtta aataaaatag
    30301 aagaaaatgt aatgaatatg ttgattaact ataattggcc gggaaatata agggaactgg
    30361 aaaatgcagt tgaatatatg atgaatttgg ttggagatga tggaataata tataaagata
    30421 tgcttccatt ggatatttta aattattata atattaatgg aaatgtttgt aaaaataagg
    30481 atataaatat aatatttgaa gatgacatag ttggagggat agtagagaat caggaaagga
    30541 tactttcaat taaagaatta gagttgactt acataaacaa gttgttgaat aagtatggaa
    30601 gagatacaaa gacgaagaag aagatagcaa aggatttagg tataggtttg gctacactat
    30661 atagaaaatt ggaagaggaa cagtagtaat ataatattaa aatgataggt atttgtgtgt
    30721 ttttatagat aggtattttt actttatggt ggaggtgtta ttatgcaata tgcgatagag
    30781 ctatattatg ataaaaaaac ggaaaagcaa ctttttgatt tatctaaaaa aatcgctgat
    30841 gaaaaaatta gtacgaagta cttagaatgg aaaacaagac ctcatcttac tttggcttgt
    30901 ttcaatgatg tggatgaggc ttgttgcata gacaagctaa aggggtttgc acaaaaccat
    30961 aaggtaatac ctgcttacat tggttcagta ggcatgttta atgatactaa aacggtgttt
    31021 gtatcaccta taatgaatag cagtatgtat cagtttcaga gagaattata tgaggtacta
    31081 caagattttg attcaaatgg ttgggaatgg taccacccca atcgttgggt accccattgc
    31141 acaatagcac ttactggaga tgatgaaagt gaggcttttt ttcgggcaag tgatgtatta
    31201 ctgcgtgagt tcaaaaaaat aagtggtgag tttgtctcaa taggattggt taaaattaca
    31261 tttcctgtgg aagaaatttt tacagtgaat atgaataggt aactttaact ctgttgtgga
    31321 tgttatgaat attttgcata gtgcatttca tcagataaat gcgaagttgc gaatttcgac
    31381 agctcaatag tcgcaagtag gacacaatgg tacacatatt gcacttttgc ttattggagg
    31441 gacgcccatg taaacttctg taagaagcta tgaaaagatt taagccattt tggaggcttt
    31501 gggtaataaa gaatatttgg gtaataaaga atataaagta tataaattta tagagtttat
    31561 gcgtaagaag gttatagcca tatatttata gaaaataaaa gtagtaatat atctgacaaa
    31621 acaatagaca tataaaaaat aagagcataa taaaggaaga attaaaataa ataaacgatt
    31681 tctcgaattg ataat
//
LOCUS       DAEPOG010000023        31226 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEPOG010000023 DAEPOG010000000
VERSION     DAEPOG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.023"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..201)
                     /locus_tag="KR432_000959"
     CDS             complement(<1..201)
                     /locus_tag="KR432_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639510.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYIS"
     gene            582..1622
                     /locus_tag="KR432_000960"
     CDS             582..1622
                     /locus_tag="KR432_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0639511.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(2085..3440)
                     /locus_tag="KR432_000961"
     CDS             complement(2085..3440)
                     /locus_tag="KR432_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH0639512.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(3466..3735)
                     /locus_tag="KR432_000962"
     CDS             complement(3466..3735)
                     /locus_tag="KR432_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0639513.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(4025..5965)
                     /locus_tag="KR432_000963"
     CDS             complement(4025..5965)
                     /locus_tag="KR432_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639514.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(6149..7624)
                     /gene="spoIVA"
                     /locus_tag="KR432_000964"
     CDS             complement(6149..7624)
                     /gene="spoIVA"
                     /locus_tag="KR432_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBH0639515.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(7796..9091)
                     /locus_tag="KR432_000965"
     CDS             complement(7796..9091)
                     /locus_tag="KR432_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0639516.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(9275..10684)
                     /locus_tag="KR432_000966"
     CDS             complement(9275..10684)
                     /locus_tag="KR432_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0639517.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(10736..11875)
                     /locus_tag="KR432_000967"
     CDS             complement(10736..11875)
                     /locus_tag="KR432_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0639518.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            12194..12844
                     /locus_tag="KR432_000968"
     CDS             12194..12844
                     /locus_tag="KR432_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0639519.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(13001..14020)
                     /locus_tag="KR432_000969"
     CDS             complement(13001..14020)
                     /locus_tag="KR432_000969"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0639520.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(14051..14665)
                     /gene="plsY"
                     /locus_tag="KR432_000970"
     CDS             complement(14051..14665)
                     /gene="plsY"
                     /locus_tag="KR432_000970"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH0639521.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(14700..16019)
                     /gene="der"
                     /locus_tag="KR432_000971"
     CDS             complement(14700..16019)
                     /gene="der"
                     /locus_tag="KR432_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH0639522.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(16053..17381)
                     /locus_tag="KR432_000972"
     CDS             complement(16053..17381)
                     /locus_tag="KR432_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBH0639523.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(17619..17819)
                     /locus_tag="KR432_000973"
     CDS             complement(17619..17819)
                     /locus_tag="KR432_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639524.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(17821..18753)
                     /locus_tag="KR432_000974"
     CDS             complement(17821..18753)
                     /locus_tag="KR432_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0639525.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(18798..19730)
                     /locus_tag="KR432_000975"
     CDS             complement(18798..19730)
                     /locus_tag="KR432_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0639526.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(19785..21074)
                     /locus_tag="KR432_000976"
     CDS             complement(19785..21074)
                     /locus_tag="KR432_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639527.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(21103..21795)
                     /locus_tag="KR432_000977"
     CDS             complement(21103..21795)
                     /locus_tag="KR432_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639528.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(21810..22571)
                     /gene="pgeF"
                     /locus_tag="KR432_000978"
     CDS             complement(21810..22571)
                     /gene="pgeF"
                     /locus_tag="KR432_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBH0639529.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(22568..23029)
                     /gene="nrdR"
                     /locus_tag="KR432_000979"
     CDS             complement(22568..23029)
                     /gene="nrdR"
                     /locus_tag="KR432_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBH0639530.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(23051..23314)
                     /locus_tag="KR432_000980"
     CDS             complement(23051..23314)
                     /locus_tag="KR432_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0639531.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(23418..24191)
                     /gene="sigG"
                     /locus_tag="KR432_000981"
     CDS             complement(23418..24191)
                     /gene="sigG"
                     /locus_tag="KR432_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBH0639532.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(24283..25005)
                     /gene="sigE"
                     /locus_tag="KR432_000982"
     CDS             complement(24283..25005)
                     /gene="sigE"
                     /locus_tag="KR432_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBH0639533.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(25030..25902)
                     /locus_tag="KR432_000983"
     CDS             complement(25030..25902)
                     /locus_tag="KR432_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH0639534.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(26193..27467)
                     /locus_tag="KR432_000984"
     CDS             complement(26193..27467)
                     /locus_tag="KR432_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0639535.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(27751..28908)
                     /gene="ftsZ"
                     /locus_tag="KR432_000985"
     CDS             complement(27751..28908)
                     /gene="ftsZ"
                     /locus_tag="KR432_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH0639536.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(29098..29451)
                     /locus_tag="KR432_000986"
     CDS             complement(29098..29451)
                     /locus_tag="KR432_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBH0639537.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(29550..30224)
                     /locus_tag="KR432_000987"
     CDS             complement(29550..30224)
                     /locus_tag="KR432_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0639538.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(30229..30927)
                     /locus_tag="KR432_000988"
     CDS             complement(30229..30927)
                     /locus_tag="KR432_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0639539.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(30950..>31226)
                     /locus_tag="KR432_000989"
     CDS             complement(30950..>31226)
                     /locus_tag="KR432_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBH0639540.1"
                     /translation="NYKTKENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIK
                     ILLSNDDNLDYNISRVSKILIDLQNKNTNGGTINLNYGKLAVYSPEG"
BASE COUNT         9655 a         5705 c         3332 g        12534 t
ORIGIN      
        1 actgatgtat gtagatagat aattactttg gtcattggca tctttttcta tagttccttt
       61 tacaggaatc tttgcatcaa ctttttcatt ttttaacacc aacgtatcta catttttgct
      121 aattatcatt actaatatat taaatactaa atatataaga ataccaagta tcactaatct
      181 ggtatatttt acctttttca aagactctcc ccctataaaa ttactttcat tataagtact
      241 tcttctaaaa aatgtttatt ccttttttat aagctaatat tttacgttct aataattatt
      301 ttttccatat taaatttatt ttttatcaat cttcctatac aagactgcat tatttatata
      361 taattgtatt ataaagtata agtttaattt acattttata cattaagctt actttatatg
      421 tttttatagt tatttttatt tttagatgca atataaaacc aattaagatt taaaataact
      481 tttgttatgt tgacatttat ttaggtaatc atttacgaat cgggataacg ttatcataac
      541 aaaagtttat gtcctaaaaa aaatacaagg aggattttct tatgtcagaa attttattag
      601 gaacaggttt acttttattg gttctatgtt gtttcacaat ttttagttac aaggccccat
      661 atggtatgaa ggctatggga gctctagcaa acgctgcatg tgctactttc ttagtagagg
      721 cattcaacct ttctttactt ggtgatgttt taggtatgga attttttaga agtgttggag
      781 ctgctaatgg tagtcttggt ggagttgctg ctgctatact tgtgccttta gccttaggtg
      841 tatctccagt ttatgcagtt ttaataggat tatcttgttt agatttttct atattatctg
      901 gattcgttgc tggatacttt gtttctttct taattaagtt tatagagaaa aaagtgccac
      961 ctggagtgga tttagtggtt gtaattcttc tatgtgcacc gctatctcgt cttatagcat
     1021 caaatgtaga cccattagtt aatagtacac tactacaaat tggacaagtt ttgatagaag
     1081 ctaccaacat tagccctata ataatgggaa ttatactagg aggcataata acagtagttg
     1141 gaacgtctcc acttagttca atggctctta catctataat tggtcttact ggtcttccta
     1201 tggcaatagg agcattaggt gcttttggtt cagcttttat gaactcaaca ctatttgcaa
     1261 aattgaaatt aggttctaaa aaagatatta tatctgttgc tattgaacca ttaacccaat
     1321 cagacataat ttcagctaat ccaataccta tatattctac taatttcttt ggaggagcct
     1381 tttctggtat cattgtagcc ttgatgggtc ttgtaaacat gacaccaggt acagctacac
     1441 ctgtggctgg ttttgctata atgtttgctt ataatccagt tcaacaagtt cttataacag
     1501 ctggtttatg tgctttattc aatgtaattg caggattttt aggttcaaaa atattcagta
     1561 cctttaaagt tcttactgct ggtgaaatac gtggtactga tgtagaagaa gaaattgctt
     1621 aaaaaacaat aagatttata aatttatatc tcaagtagta gagttttata ttgtaggcaa
     1681 ttttattaat tgcaaacgtt ttatttttta tttatgttta tgattattat gtgtgttgaa
     1741 tatttagata aatttttatt tattattttt atttagattt ttgtttagtt ttttatttgg
     1801 ttgatatatt aggaataaaa attagtatag tttattatta gttagatagc ataacttttt
     1861 agatacataa aaaattttat attaattaga tattattaaa ataaaagcga tacatgaatt
     1921 tttaaatgtc catgtatcgc tttatttttg ccataatctt aacaactgat ttttaaagtt
     1981 tattatctta acgaagttca ttatcttata taatatttaa atgcatagac ttctttaatt
     2041 taaccatcta aatatttaat taaatattat tatatgtaat ataactaatt tttaaatgaa
     2101 tgtataggag ctggaatcct tcctcctctt tgagtaaata attcagatga atttgtactt
     2161 ataggcatta ctggtgctcg tcctaaaagt cctccaaatt ctaccatatc acctacatca
     2221 catcctgggg ctggtataat tctaacggct gtagtcttat tattaatcat acctattgct
     2281 gcctcatcag ctatcatagc tccaagcgta cctgctgtag tatttcctgg cacagctatc
     2341 atatcaagac ctacagaaca aatagcagtc attgcttcaa gcttatctat acttaaagca
     2401 cctttaataa cagcatcaat catacctgca tcttcagata ctggtataaa cgctccacta
     2461 agtcctccaa catgtgaaca tgccattact ccaccttttt taacagcatc atttaaaagt
     2521 gctaatgctg ctgtagtccc atgagtacca acaacttcaa gacccatttc ctctaatata
     2581 tttgctactg aatcaccaat agcaggtgta ggagctagtg ataagtctat tatcccaaat
     2641 ggaacatcca atctactaga agcttccttt gccacaagtt gtccaactct tgtaatttta
     2701 aatgcagtct ttttaactgt ttctgaaaca acatcaaaag gctctccctt tactttctct
     2761 aaagctctct taacaacacc agggccactt acaccaacat ttataattct atcagcctca
     2821 ccaacaccat ggaaagctcc tgccataaat ggattatctt ctactgcatt acaaaatact
     2881 actaattttg cacaaccaaa accttttgca tcttttgtta attctgcagt ttcttttatg
     2941 atttctccca tttctttcac tgcatccata tttataccac atcttgtaga acctacattt
     3001 actgaagcac atactttatt agtacttgct agagcttttg gaattgactt aattagtatc
     3061 ttatcacctt ttgtatatcc cttttgaact aatgctgaga atccaccaat aaaatctata
     3121 cctaaatcct ctgcagcctt atccaatgtc tgtgcaaact ttacatagtc attttcatct
     3181 gttgcacctg caataatgga aattggagtt actgataccc ttttatttac aattgggata
     3241 ccaaattcac gctctatatc ttttccaacc ttcaccaaat ctttagcaga actcataatc
     3301 ttattgtata ttttttctct agctttatct ccatcagggt ctatacaatc taataaagaa
     3361 atccccatag ttatagttct tatatccaac tgttcttcat ctatcatctt tattgtttca
     3421 agtatatttc ctacattcac tattatcacc tccccttact gtctactaca gattatgcat
     3481 tgaattaaat atttcttctc gctgaatttt tacatccact gataatttct tacctctttc
     3541 aaccaagtcc tcaaccgttt tctcaaaaga atttaatgat ttctctaaat caactatcat
     3601 aatcatagta aaataatcct gtaatatttt ttgtgatata tctactatgt tgatattttg
     3661 cttgtataat tcatcactaa ttcctgccac tatacctggt ttatcctttc caattactgt
     3721 taaaattgct ttcataataa tatcctccct tattttttta taaatcttat atttttacaa
     3781 attgaactat gtattaacat atatttcatt tatttgttag taaaccactt tttatgtata
     3841 tatttaatat attttaatac ctatacattc atttgtcaat tttttttcaa tattccatta
     3901 tttttatact atttagaaaa ttcatatttt atatatgttt tctcaacaat aaatacagtt
     3961 ttcataataa tgaaaggtat tacttagcaa tctcgtgatt ttctaaataa tacctctcta
     4021 aaaattattt ttttaatttt atgaatttaa ttatactttt tctagtatca ggatttacaa
     4081 ttaatataga accaatcata attggtgtta gtatacaaaa taaaaaaata atatcttttt
     4141 ttagattata ttctttagat tctaatttat aattattatt tattattcca tcataatcac
     4201 ttatatttga cttattttta tttgtatctt gatttttgtt agaaatactt tccctataag
     4261 gaaaagaatg ttcattggta ccaaatactg attttgagct taaatcataa ttatcatcat
     4321 tttctgtaat tggattttta ttgtaaaatc ctttttcttt atcagaatta ttttctttat
     4381 taccgtcttt aggtatatca tgaggatttt tattttcatt gctatcagta cttgattcta
     4441 catcatcttt aggagtattt ggttgtttct catcagaatt attattttca ttattattgt
     4501 tttgattgtt attactatca ttattttcat ttccattatt cccatcttga ttgttatcat
     4561 cctcatctct atccgggtcg tctctacctc ctcctccacg gtcaccatca atatcatcat
     4621 tttcccgttc atcatctggc gtctcttggt tgtctccgcc tcccattata aatcctggtt
     4681 taaaaactat atttgaatac ccttctttta atcctcctac cacttttact ctaaaacaac
     4741 gcaacttaaa atcttcaaaa aaaagtatag cttgatctct aatatcctcc tgttcttcag
     4801 atatccaatt gattccatcg cttgaatatt caacaaaaac cttacttgca ctatacggca
     4861 tatcataaaa taaaacagct acatatccat tttttgtatt ttgaaattcc agttccaaat
     4921 tgtctattgt ttttttctct ataacagata cattcaaaat aggaactaca tcatcattta
     4981 ttataatttt ttctttattt aacatttctg ttttaaactt tcctgttata tcaaaatttt
     5041 ctcccttttc caacttttca taataatcag taaaatccca ttctacatct atatcaagtt
     5101 gattaccact agatgtttct aataccatat atttgggaaa ttttatattt tctattttat
     5161 catctttact tacttttatt tcatcactaa atacaccaat tcccctatga caacatttca
     5221 caatattata gccagaatct ttagctatag agggtataag ctcattataa tcatcaatta
     5281 tataaacttt cttactttct gatataattg attgtgccaa cttattatta tctcttagta
     5341 acccactttc attgttgcta ctatatgctt ttattgaagt ttcttctatc tcaacatcac
     5401 ctttagaata aacacctata gcatgttctc cattcacttc aatatcctta tttagtattc
     5461 taatatcatt ttcagtataa attcctattg catcttttcc atttgccttt attttttcaa
     5521 cagattcgta ggatgattta atatgtaagt ctcctccttc ttcaacgtat aaggctgtcc
     5581 catcagtagc aattatgttg tttataccaa tacttatact tccaccattt tctatatgta
     5641 ttactccttc ttttcctcct tcacctatta tattcaattc attatcatca ataataaatc
     5701 tcccatgatt ttttatcaat attttatatt tgttggtatc tattgtaaaa ttactgtcat
     5761 aaggaatata aaatctaaac tcttcatcta ttaccatatt acaagaaaga gtataaacta
     5821 tttcttcttt attattttta ttttcttgaa cccacctttc aaaatcttca agacaatttt
     5881 cctcttgtcc aaaaacttta tgtggaaaac ttataaaaca caaaactatt atactcataa
     5941 aaataaatat ctcatacttt ttcatcttac actcctagaa attttttagc tttgaaacaa
     6001 tcctgtcgaa acatatactt cacccactat aatttttcca caattatcaa ttattgtcaa
     6061 tttatataag tatctttatt aattgttata aataaaaagc tacttactaa agtaaacaga
     6121 cgtttcactt taataagtag ctatattttt ataacaaaat agttataata ttagaacttc
     6181 cttcatttac aattttttgt aaagtctttt gcattttaac tcttatctct tctggcatag
     6241 tatataattt actttgtaat tgctctttta ctaagtcatg tagtgactta ccaaacatat
     6301 ttgattccca taaatctgct ggttgctctt caaatacttc cataagatat tttatcatct
     6361 cttcaccttg attttgattt ccaactattg gagagacctc tgtagatata tctgctttta
     6421 taatatgaag tgatggtgca ttggctttta acttgatacc atattgtttt ccttgtttca
     6481 ttatttctgg ttcttccaga ctaagttctt ctaaagaagg agctactact ccatatcctt
     6541 ttatcttggc atctattaaa gcactttcta ttttatcata ctcattttta actttagata
     6601 gtctagttat cagactaagc agttgataat ctccttcaat tttaaatcca cttttttctt
     6661 caaggacatt atagaataaa tcttgctttg tctgtaaatc aatatttata acaccttctc
     6721 ctagctctac attatcaaca cctgtatctt ccaaaaactc taactcagaa aatccttgta
     6781 taataccttc tatatctctt attttaccta tatctataat actttgtttt aatgttgtta
     6841 ttatgctact ttttatccaa tgatttcttt caagaccttc aacccatttt ggtaagttga
     6901 ttcttatttc tgtaagtgga aaatcatata atacagcttc cataacctcc tctatatctt
     6961 cttcttccat ttcaaccaca ttcattggaa gaactggaac ttcatacttt tcttccaatt
     7021 cacttctaag catactcgtt tcctcacttt ttggacttaa cgtatttaaa accactgcaa
     7081 atggtttttt aagatttttc aattcttgta taactctctc ttctggttct acatagcttt
     7141 ttctgtcaat tccagtaact gaaccatctg tcaagacaac tatccctatt gtagaatggt
     7201 cttttataac ttttttagtt cctatctctg ctgccttttc aaatgtcata gcttcttgtg
     7261 accaaggagt ggatactaat ctttgttttc caccttcctc atgccctaaa gctccttcaa
     7321 ctatgtatcc aacacaatct accattctta cttttaaaga tactgtgtct tttattttta
     7381 tttctacacc atctgctggt acaaattttg gttctactgt cattatcgtt ttacctgaac
     7441 cactttgggg tatctcatct cttgttctat cctttttaaa ttcattgtct atattaggta
     7501 taaccaactt ttccataaat tttcttataa aagttgattt tcctgttctt acaggtccaa
     7561 ctactccaat atatatatcc ccctgagtcc ttttggatat atcttcgtat atgttattat
     7621 tcatattatt cctcctatac atctaatctt attaaattat atttagagtt aagggttaat
     7681 attcttaaaa tagaaaaata aagaccagca atattaattt aatttacata tagaaatttg
     7741 taaatttcaa tgtaaattaa attaatattg ctggtccaaa tagtagtgta ttatattaac
     7801 tgactttagt agtattttca tttgacttat tattcatctc aataagttct ttacgtcttt
     7861 cttttgtcat gttcatccat ataaatgtaa acaccaatcc aattatggct actatagtaa
     7921 gaactatgaa gtagtaccta tatccagtta cacccggaaa tgcatccaat atcttccctg
     7981 ctgctaaagg acaaagtact tctggaagat atcctaatgt agcaactatt cctatagcag
     8041 ttcctgatat tgataatgaa taattacctt cttcaagtaa cgaatagtgt aaaccttgaa
     8101 ttgcatacat agatacatat attgcaacac atccaatcaa tagtatataa atcatagatg
     8161 attttgttgg tgtaaatatt acacctaaaa gacctgcaat cataagtata aatccataag
     8221 atataatctt tgatgaacct actttatctc caagtaatcc agaagcagct gatgctactg
     8281 gtctacaata ctgagctaat attgagattg cagatgcaaa caccacagta gctccaaatg
     8341 cttcagttga ataaggcgta aaataataaa atgacatatt agctgaatat gaacaaaaca
     8401 ttattattgt tattatccat gtatgaggca tttttattac tgcaaaaaag gcttctttat
     8461 caaattttgc tgatacctct ttttcattat cctttaattt gaaaaatact aatactccaa
     8521 ctattacatt tattgccgag tataatacta taatagctgt aagaccagat ttatcactta
     8581 cttttgatga aatataccca aatagtatca atactagaga tagatgaatt gcattggtaa
     8641 ttccacgacc actttccata aatccaaaag cttttccttg ttcattttca gatgctaaca
     8701 ttctaacagc ttttacaaga gctggccaaa aagtaagtat tgttgttatt ccccataagg
     8761 catggattaa aaacacaaca atatatggag gataaagtgt taatacaaga ccagatattc
     8821 ctgtaataat taaagaacct gaaagcaatt ttctggctga aatacggtct gctaaaaaac
     8881 caccaaaaag gtatgatacc attgcaagta gaccataaat gcttcctaag cttcccattt
     8941 gtgtatttgt aagattaaat gcttgtgcaa aagtatcata ataataattc tttaaatatg
     9001 gtaaagcata tataaatgct cctgctacaa agagtattat caagactttg aaatttttcc
     9061 aaatgcttgg tttatttact tcaactgaca tacttcctcc ttatgtaaat acctgtaaat
     9121 tttcatgata attaattatt gcttatttta ttgtttattc aatatttcgg aatatttatt
     9181 aatatattag gttaatctat caataattta tggtgagtta tgagctaaaa gtaaactctt
     9241 ttagctcacc ataataatta tatttttatt tatactaacg tccagagtta ttagatatct
     9301 ttttttcata gttcttccat agcattaaac ctgttattat aacaaaaaga cctcctccta
     9361 tcatttcaat ctcatagatt atattatctt ttaaagtctc cataactggt gccgttgaga
     9421 aaagtatgcc aactacagtt actacaagta ccattctggc aatattgata caaactgaat
     9481 cactttttgc aattgaatat ggtctttctt catttggtct tgtctttcta agtctaatat
     9541 aagatgtaaa aagcaagaca tatggaaata atgctgttaa tgctgtcata gtaacaagaa
     9601 cattgtatat attatctact gatggtaata agtttgttcc tacaagaagt agactcacta
     9661 ttatagcctg aactattact gcatttgatg gtatattgta tttatttgta acagtcaatt
     9721 gcttaggaaa tacaccttct cttacatttc caaaaagcat ctttactgga cctgctatat
     9781 aaagtattaa tgctcctagt actgataaag ttattccaaa tgctactatt cttataaacc
     9841 aacttcctat acctaaatct tgtgcaactt tagctaatgc atctagtatc cctgtagacg
     9901 ctgcaatttt atctggagga agtatcattg ttattgctac tgaacctaag atatagattc
     9961 ctcctattaa aacagccaca gttaatatag ctttaggaaa gtttttctta gcattatcca
    10021 tttcggttat gaaattagca gtcgtttcag ctcctgataa tgcaaatatt atagcagata
    10081 tagaaactaa tgaattggca tctatttttg gtattatatt ttggattgta tatatcgatg
    10141 ctgatggagc ttttttgaga ataactacag acataaatgc catcactata agtaatatag
    10201 caggaattgt agaacctaat gctcctgtat tcgtaaaaaa ttttccaaat gccatcccac
    10261 gagtacttac taaggacaac agccaaaata ttatgagtga aagtgctaaa ataaacatct
    10321 tattgtttga taaatctgga tttccaagca tatatgcaat atttatagct aaaaatgtca
    10381 agaaagaaga ataccaaaat atctttgatg tccaatttag ccatgacacc ataaatcccc
    10441 acttctctcc ataggcttgt ttaacccatt ctccaagtcc accatctttt ggatatgttg
    10501 cagccaactc tgcacatata agtgatgcag gaacaaagaa tataaaagca aacaataacc
    10561 aaactggtat agaaccaaga cccaatccaa aactagaggc agtagattta gctatccatc
    10621 taatcccaaa tagtgctgat atattcataa atactaaatc ccaaaatcca attttctttt
    10681 tcactgtaat tcctccaatt tcaaatttat tcaaactcat acacaatgta ttgatttaaa
    10741 tatcaagtat aagttcttca acacttcttg gataattagt tataattgta gcaccattct
    10801 tacctactat tgctgtatct gagtgtctaa atcctccaac tccaggtata tatatccctg
    10861 gctccataga aaataccatt ccttcttcta atattaattc attgtcaaat cttaaatatg
    10921 gctcttcatg ttctgataaa cctaaaccat gaccaattct atgatttgaa tataactcta
    10981 gaccatactt tgcaacaaca gctcttgctg cttcatccac cattcttgct ggtataccag
    11041 cttttattgt atctaatcca gcttgttgag cctctactgc aatcttaaac acttcttttt
    11101 gtctttctgt tggtttacct ataataaaag ttctttcatt ttctgctcta tagttttcat
    11161 accaaacctg tctactatgt attactatat ctcctctttg aagtatccta gtattggaat
    11221 ctaaatgagg ttgtgctgtt ctatcaatcc ctgaacaagt ccagtttgca aatcctatat
    11281 atgtatcagg ataattttca gaaacatatt ttagtaaagc attatccccc gcaacgtcaa
    11341 actccaattc actcatccca actctaacat tttctaaaga cccttttatt cctatatcag
    11401 ataggtaacc tgctatcttc aaaaattcta tttcttttgc atcttttaca tatctcattt
    11461 caaatatttt acttccaaca tctttgatag caaatgactt atcagttata tactctgaaa
    11521 aacttgcaga tactatatct ttttctattc caacagttgt tcctttaggg tatttattta
    11581 aaattatatc taagtaatgt ttatgtgata ttccatgttc tttcatttct ggtatttcat
    11641 aatacacata tacattatca acttttgcaa cttctttagc atgtaattct tctaatccag
    11701 gtactattaa ttctatttta tcttgagtta ctactgttac aattggtcta gaataagtta
    11761 ttgctttaaa tccactaata taaaattgat tgtctggttc aaatattaaa gcaatttcaa
    11821 tgcttttttc attcatgtac tttctcaatc tttctagtct catattattt gacataatgc
    11881 acctctttct taatattcag ataatttatt caatacaaca aggataaata tattatttta
    11941 aaattcttaa tatttttagt acttgtcgta tttgcatgta aattaatttt atttgtgatt
    12001 attttatcat aatcatgatt attttgtcaa tcacgatttt atttttaaaa tttttttata
    12061 ttctagtgat tttaaattat ctaaaatata acgagtatac attgtatttt ggaatattat
    12121 tcttgattta tggtaagttt aatgataaaa tcacgattat ataaactata ttgattattt
    12181 gtaggtgaat aaaatgacaa ataaagaatt acaaaagaaa aggatattga tgtatttcat
    12241 agaagctgct caaaatatta tggaaaacga aggtatagaa aatattacac ttcgtaaggt
    12301 tgcagatatg gctggatata atagttcaac tctatataaa tactttaaaa atttagacca
    12361 tttaatatca tttgcatcta taaaatattt taaagattat aacttgaata tttcaagatg
    12421 tatagaaaat gtcaatgatg aatataagaa atatataatt atgtggaaat tattttgtaa
    12481 gcattctttt gacaatgctc aagcttttta tcaggtattt tttaacttgt ctagtgatga
    12541 acttagctat ataacaaaac aatattatga tatgtttcca gaagatttag gtattcatga
    12601 tagtgacata tctttaatga ttacaggaga aagtataaag gaaagaaata agatactatt
    12661 aaacaatctt gtagatactg gatatatacc agctaaagat ttagacataa tcaatgatat
    12721 aattatttct ttttatcaaa atctattatt cttaaaaaag aacacaggaa aaaattctgg
    12781 tgatgatgaa ttaactatga aaatgatttc ttctattgaa tacataataa aacatgctaa
    12841 ataaaattaa aatatggctg tctcaaaata gagataagcc ttttttattg ttgaaataat
    12901 aaaaatatat ttaatttcat ataaaaaaaa gtgcctcgta aactaaaaag ttcattttga
    12961 gacaccccta aaacaaattt attatttatt atacaatagt ttatatatca tcacctaaaa
    13021 ctacttcttc catttcatgc tttttacttc tcatcattag ttctattcca acttctttag
    13081 gatttgaacc tttatttatt acagaatata tagcattagt tataggcata tctatatcta
    13141 atttttcagc aacatcatgt gctacttcag tagcagttat gccttctaca accatcttta
    13201 cttcttttaa agtatcttct aaactcatac ctttacctat gagtatacca gctcttctat
    13261 ttctactatg catacttgta caagtaacta ttaaatctcc aatacctgaa agacctgcaa
    13321 aggtagacat attagctccc atagcaattc caagtctgct catttcacta atacctcttg
    13381 tcataagggc agcttttgca ttatcaccat accctaatcc gtcacatatc ccagctccaa
    13441 atgctataat attttttaat gctcctccaa gttctactcc aactatgtct ggattcgtat
    13501 aaactctaag tttagggctc ataaataaat cttgtatcat ctgtgctatt tttaaatctt
    13561 tagaagcaac tactacggta gtaggaatat cccttgctac ttcttcagca tgagatggcc
    13621 cagatagtat tacatatgga ttttgtggta attcttcttc acagacttga gataatctaa
    13681 gcccagtccc tttttcaaga cctttagcta cattaacaat aacttgtcct tcttttataa
    13741 atggttttat tttttggcaa gtacttctta cagcctgtga tggaactgct aaaactatta
    13801 ccttacaatc ttttatagtt ttttctatat cagtacttat agttatattg tttggaaata
    13861 atactcctgg taaatagtct gtattttctt tagttctgtt tattttttta gcttgttcct
    13921 cttttcgagt ccacatgctc acatcattac ctttttttgc aagccctaaa gctaatgcac
    13981 ttccccagct tccagtacct aatacacata ctttctccat gtttaacacc acctgagtat
    14041 aatatattta ctattctttc ttttgaccaa tttttctctc tgtcccattt aataatcgct
    14101 ttatatttgc tctgtgatta tatattactg atgcagttaa gaacagagct actattaaac
    14161 cagctttatt ttttaccata atcataaata ttggtgaaca tcctattcct acaacagaac
    14221 ccaatgaaac atactttgtt atagctacca ccaatataaa tactgctaga cacattagtg
    14281 ttattactgg atttacagct agcattgcac ctagtgaagt tgcaacacct ttacctcctc
    14341 taaatcctaa aactgctggc caattatgac ctgctacaac acatataact gccagatatc
    14401 cagctaaaag agtatctatt ccaaccaatc ttgctgcaaa ttctgatatt aaaacagcca
    14461 ttacaccttt aagaacatca cctaaaaatg tcattgctcc tgctcttttt ccaagtgtcc
    14521 taagtacatt tgtcgaacct gcattaccag aaccttgagt ccttatatca accccagcaa
    14581 ttcgctttgc aactatgtat gaggtagata tatttcctaa aagatatgca actacagcaa
    14641 taattatata actaaatatc tccactgaat ttcccccctt tagttatatt ttaagatatt
    14701 tattttttat ttttttgtct atactcaaac tttatggaag ttccttcaaa tccaaaattt
    14761 tctcttatct tattctcaag gtatctttga tatgagaagt gagttaaatc cttatcattt
    14821 ataaacaatg taattttagg aggtctaatg tcagtttgag tcccatagta aatttttagt
    14881 cttctacctt tgtcagatgg tggttgattt aacattacag cttctccaat tacttcattc
    14941 agtgctgaag ttgatattct cttagaatgt tcatttgaca catattcaac tgtatctagt
    15001 atcttgttca ttctttgatt agtttttgca gaaacaaata cgattggagc atacatcata
    15061 aatggaaatt tttcttttat atcttttgtg taattactca tagtcttatt atctttctct
    15121 attaaatccc atttattgat tacaaatatg catccttttc cttcatcatg tgctatacct
    15181 gcaacctttg tatcttgttc tgttacacct tctaaggcat ctattactat caatactaca
    15241 tctgctctat ctacagcact catagctctt attacactgt atttttctat tgtctcatat
    15301 attttacttt ttcttctaag ccctgctgta tctattagaa gaaatttctg tccatttttt
    15361 tcaaaataag tatcaattgc gtctctagta gttcctgcaa ttggacttac aattactctt
    15421 tcttctccaa gtatcttatt gagtatagaa ctttttcctg catttggttt acctgttata
    15481 gctactctta ttatatcttc ttcatactct gtatctaatc ctgctggaaa attttcaact
    15541 atctcatcta ataaatctcc aaatcccata ctattggctc ctgaaactgc aaaaggcgtt
    15601 ccaaatccca actcataaaa atcatatata ttatcaaatt gactttgact atcaatctta
    15661 ttaaccacta agattactgg tttttttgtc tttctaagca attgtgcaat ttctttatca
    15721 gcagctgtta ttccagcttt accatcaact ataaaaagta ttacatgtga catgtccatt
    15781 gcaagcatgg cttgatttct catttgagat aatattatat cttcgctatc aggttcaatt
    15841 cctcctgtat ccactaatgt aaaatattta tctaaccact ctacttcagc aaatattcta
    15901 tctcttgtaa ctccaggtgt attttcaact attgatatcc tttttcctgc aaatttatta
    15961 aatattgtag acttaccaac gtttggtctc ccaactacag ctacaactgg cctacttata
    16021 ctcattttat ttctccttcc atcttttgtt ttctatttta aaattttatc cacaaagtct
    16081 tttccttcat tcaaacaagg gataattttt attctcataa gttcttctaa ttgatttaat
    16141 tcaatattat ctaagaaaat ttcttcatct gcttttagca tacttcttgt tatataaaga
    16201 gcttctccta attctttatc ttcaagttgt tcttttaaat ccttagctgt taaaagacct
    16261 gatacagtta ttgtctctcc aaaaaacttg tttttgattt catatacatt tacttgaaga
    16321 tttttaaact tgtccatcat agcatctgcc atactctgta taaattcata tgcagaatga
    16381 ccagtagcta ttgatacttt cttctctctt tttagaatat tctcaggcaa tatatttaga
    16441 tactcttcaa tctctgtttt taacttacgt atcataccta caccatcttc aaactgtaaa
    16501 aaaccttcat attcttcata tccaggcaat tcactattag atagtatata aaattcatct
    16561 gataaaaatg caaatcgagt acctaatttt tcaagatatt tctgttgtat ttgatgtatt
    16621 tgctctatag ttttacttgc gctcttatca ttaaatatat ttaattctac tagattttct
    16681 ctatgctttg taatcccaac tggaactatc gcaacactgt ttacatatgg ataaagttga
    16741 gctagttctt ttattgttct atccaattcc tttccatcat ttatacctgg acataaaaca
    16801 atttgacagt tcatttctat atgagcatct gccaatctct tcattatacc atataatttt
    16861 cctgcaaatt tattgcttat cattttttgt cttaactctg gattagttgt atgcacagat
    16921 atatttatag gacttattct atattttata atattattta tatcttcttc actcatattt
    16981 gtaagtgtga caaaatttcc ttgtaaaaaa gataatctcg aatcatcatc tttaaaataa
    17041 agagtttctc tcataccttc tggcaattgg tctataaaac aaaacataca cttatttcta
    17101 cagcttttag cttggtcaat tatcggattt gtaaattcaa cacccaaatc ttcatcatag
    17161 tctttttcta tctcgtatat gtagacttcg ccatcttttt tttgtatttc tacatctaaa
    17221 tattcatcac ttagtaagaa tctatactct attatatcat gtattggctg ttcattcaca
    17281 gatataagta aatctcctac ctctatacct atttcttctg caatactatc tttataaacc
    17341 ttacttataa tattatttac tttttttaca tttacttcca atattttcac cactcttttg
    17401 tattcatcaa tgcttattat aatatatttt aatatgcaat ttatttattt attatatttt
    17461 atgatagcat ttttaagcat ttcatgtcat aataacacat tttaattcta atagtcaata
    17521 taagtaaata gctataaaat agattagaac aaataatatt caaatttaag tttcttaaaa
    17581 atttgaatat tatttgttct taaaaaaatt ttataaattt aatgttttac tatgattaac
    17641 gtcccatttg tcatatcatg aaccattcca cctacacact ttgacatcaa caatgcctta
    17701 ttttgtaagt ctttctcatc atttgcataa aatattggac aacctccacc atttttcata
    17761 ttcttatcaa gtgtaactat agctagggta tattcattta aacctatatc cattcccatc
    17821 ttatgcactt cctttctttc ctattttata attttttagt gataagtttt ttcctttaga
    17881 gcttgaaagt attgggcatg actttacagc ttccattact ttttccatat tcttatctac
    17941 tggtaaataa gcaatcatta tactttcatc ctctttattt cttctaggta atggtacgaa
    18001 tgaaggttct ccataatctc catataaacc taaccttgaa tatatattgt atactattgc
    18061 ttgtctttga ccggagtcaa ataatattcc tgcattagca taatccttat ttttaggaat
    18121 tatttgtatt cctacacctc tattaatata tacttctcta tcacttttta gacctatatt
    18181 tgttatacct tttaaatctc ccacctttag tatagattcg tccacaaaac taatttcagc
    18241 aggcattaca tcagctatgt ctccaatact ttttctagta agaagttttt ttaatataaa
    18301 ggctaacact aatccacaaa ttgaacttat taccaaactc atcccttcac ttattttcgt
    18361 ttctgcaagt attacataat acaatcctat tgttaaaaaa gatgtaacta tacacatata
    18421 atttcttact tcataggttc tagctatctc ttctatgaat gcagcacctc tctttacaag
    18481 ttgaacttcc tctaaatttt gcaatgtatt tcttctattt gctctcactt gtctaaattg
    18541 ttctgctgct agagataaaa atgttatgga tgtataagaa cgatttataa gagctggaac
    18601 caataatgct cctaatgaag aagcgacgaa tgctaatata agttgagata gtaaaacatt
    18661 tggttgcgtt ggatactgtt tttggtctaa attcaacatt ataattctag atgttatacc
    18721 aataataaca gcaacaaaaa atactctatc cattaaatca ctcctaaaat atttaatttg
    18781 aaattttaca tgtaaatcta caaagtcttt ctagaaaaat cactctgctt tccttttgat
    18841 gtttctatga taggtgtact tttcactaca tctatcatga catctatatc tttgagtatt
    18901 ggcatatatg gtataaccac agtacttttt tctaaattgg tcttagatat agcaacaata
    18961 tcaacctcat ctacatctct atctatcccc atatgaataa atagattatg tattatagct
    19021 tgtctttgcc ctatatcatt tactattcca aatgcaccta aatctcttgg tataacttct
    19081 atggccagac attcatttct atacttttct cttgtacttt ctagacctat attagttatt
    19141 attacatcat ttaccattaa aataggtcct tcaaaactta tttttgcagg aactatatct
    19201 gctatatctg ctatactgtt tcttcttaaa aatcttctaa aaatcaagcc aacaatagct
    19261 cctgacacaa tagctaaaat tgtgcaatga aaaaagctaa ggtcatactt tcttgcaaaa
    19321 acgatataaa ctatcgaaga tactagtgca gaaaaaagac ttatataact tctagattca
    19381 tacgtagatg ctatttcttc aacataagca tctccttttg gtactaattc ttcattatca
    19441 atattcttta gtgttattct ttcttgttcc gcaagacctt gaaactgttg tattgctact
    19501 gcaaaaaatg tcaaggctgc aaactcctta tctataagtg caggtaatgc tatagcacct
    19561 aacgaagctg aaagcccaga tataattatc tgttctaaat aatcttgtgg tcttgatggg
    19621 tattgtttgt ctgtaactct taaaactaat cctctacata atattcctac aactaatgca
    19681 actataaaag agtgtctaaa taaatttgca ttcaataaac tttcttccat tttctcagct
    19741 ccttctctta tatagtttgt gcaaaaaaca aaaaaaattc ccttctattt aaggaaattt
    19801 ttttgtaata ttgtaactat aaattcgcta ccaatgccaa gttcactttt tacttttata
    19861 catccattta gtgatttgac tatatgcttt gttatagcca atcctaaacc cgttcctcct
    19921 acatccctac ttcttgcttt atctacccta taaaatcgtt caaatattcg ttctacatct
    19981 tcttttggta tccctacacc attgtctata actttaatta cgattttttc ttcatctttt
    20041 aacactacaa cttgtacaat cccattatct tgggtatact ttatagcatt atcaactagg
    20101 tttaagaaca cttgctttat atagtcttta ttggatagta tacttattga tttatcatca
    20161 catttatata aaacttctat atttttttca ctagctgaat gttgagttat ttcatatacc
    20221 tctttaaata catcatatac tacaacattt tccataagaa gagtatcttt attttctaca
    20281 aatgataaca gcaatatatc atcaatcaac ctttttaatc tatctgattc gctttctatt
    20341 atttctaaga atctatttct agtattcaca tctatatttt catttgactt tagtgtttca
    20401 acaaacccac ttatagatgt aagtggtgtt ttaagttcat gacttacatt tgcaacaaaa
    20461 tcacttctca tattttcaag tttaactttt ttagtaatat cttctatatt tactatcgaa
    20521 cctattatta cgttgttagt attctgaaga taaactgggt ctacctttat cttatatacg
    20581 atatcatctt tagtagtaat attagcagtt tcattatctt tagaaccaat aaattgaagt
    20641 atttgtttta atatttcttc ttcttttatt acaacattaa tattatgacc ttcaactaat
    20701 tgatttggtt cactctttat catttctttt gcttcatcat ttatcagaag tatatttcca
    20761 tttacatcta tagccaaaat tccatgagat atactcttta atatagatct taactgtaaa
    20821 tgtttatact gtacttcttc taaggtttta tccatattct ttatcatatg attaaaattt
    20881 ctagctaatt caccaagttc tcctttggct gatatgttaa gcctagcatg atattcatta
    20941 ttacttactt tttttgagac gtatataaac tcttttaaat atttccttaa tgttattgta
    21001 tatcttacag ctaatataac aatggctatg gacaacataa caataaaaaa atatatacta
    21061 tttaaatctt ccacaagata acactcctaa aatataacct ttttaatcta ttttataccc
    21121 tacacctctt atagtctcta tatatttttc cgatttatct tcatcttcta ttttttttct
    21181 taaatatctt atatgaacat ctactgttct agtttctcca tagtattcat acccccatat
    21241 tttatccaaa agataatttc ttgaaagtac ttttccttta ttttgaatta ataattttaa
    21301 caattcaaat tccttaagag ttaactctat cttttcagag cctttggtaa cttcatgttt
    21361 tgataaatct agttttaaat tgcctgtagt aagtatattg ttttcttcac ctaagctagt
    21421 caaattatat ctccttaaaa cagaactaat cctagctaac aactctttta tactaaatgg
    21481 cttagttata tagtcatcag cacctatttc aagcccttca accttgtcat tttccatatt
    21541 tttagcagta agcattataa caggaatatt ttttaagtcc ttatcacttc ttatttttct
    21601 caaaacttct attccactta tatttggtaa catccaatct aaaagtatta aatctggttt
    21661 aatttcctta gcctttataa atccgtcaaa tccatcatat gaataactaa cttcgtaatt
    21721 tgacacctcc aaattgaatt ttaatagctc aactatatgc atttcatcgt ctataacaag
    21781 cactttagta ttcattaaaa agctcctctc tattttatct gtagtaccgt tcctattcgt
    21841 tctgatgcct tattatgttt tctataagag tggaatttat ctgctctaca actagtgcat
    21901 aaatttaaat ttattatatt ttctttttta actccactac atctaagcat atactcatta
    21961 attttccata aatctaaata gtaacttccc tcttttatta tatagaactt ttcatcatta
    22021 tttgtaagaa ttgtgttaaa tttttctact aaatcttcag acacttcata gcaacatggt
    22081 ccaattgatg gcccaattat acatactaaa tcttcaggaa ctgttttata gcattcagac
    22141 attaatttta ttgttttatg acctatatta ctaaaagtcc ctctccaacc tgcatgagcc
    22201 agtcctatgg ctttattttt agcatctata attgatatag gtacacagtc tgctgtaaac
    22261 agtaaaagtg gtactttttc caagtttgtt ataagtgcat ctccatctct tcttttccct
    22321 atagtatctt tattgactat attaactata tctgaatgta tttgagaatt actagttata
    22381 ttttcaaatg tcaaatcaca ttctttacat acttttaaca tatcttctct attttttgca
    22441 tctatattct tacatgttat aactagattt acagaattta atttttcatc aagttcttca
    22501 caattaccac ttaaattaac atactcttta atatcttgta cattttttat atttatataa
    22561 tctttcatca tttgtctcct ttttctatca gtatactttt aagttcattt acaaaagtat
    22621 ttatatcttt aaattgtcta tacacagaag caaatcttac ataggcaacc tcatctaact
    22681 ctttcaattt atccattacc atctcaccga tttttttggt ttcaatttct tctataaaac
    22741 atttatttat ttcattttca atatgtacaa caatactttc aatctcttca atagaaacag
    22801 gtcttttttc acatgctttt agcaatccat attttatctt ttctctatca aaatattctc
    22861 tactattatc ctttttaatg accattaaag gagttgtttc tattttctca tacgtagtaa
    22921 acctcttttt acatgattca cactcacgtc ttcttctgat agattttaaa tctgtatgtc
    22981 tagaatctat tacctttgat tctttataat tacaataagg acattgcatc tatattcctc
    23041 ctttgtttaa ctatatctca aactcatcta agcttaattc tgaacctata tttactatta
    23101 ttgtatcaca acctatttta agtatatcat tccaaaatat aacttcttca tcccctcctc
    23161 ttgaaaaaaa acttctactt ccatctccaa aaatggtaaa tgagactacc ttaccttcgt
    23221 gaatatccat gtctatgtct attataaacc ccatcctttt tccatttttt acatttataa
    23281 tttctttttc cattatatca gaaactctaa tcatattgta ctcacccttt ctttaaaaat
    23341 aaatatgtat cgaagtaaaa aaaaatgacc tatataggtc atttaaatta aaataatgtt
    23401 tacagtccat ggattactta tacatatttt ctcatatttt ttaaagcatt tttttcaatt
    23461 cttgatactt gtgcttgaga tataccgatt tcatcagcaa cttctatctg agttcttcct
    23521 ttgtaaaacc ttaaatctaa taccaacttt tctctactat ttaatttctt aatagcttct
    23581 ttaagtgata tttcttgcaa ccaattttca tcagtatctt ttttatcttg aacctgatcc
    23641 ataacaaata ttgcatctcc attgtcttga tatactgggt caaacagaga tattgggtct
    23701 tgtatggcat caagtgccat tacaactgat tctacttcta attctaattc ctttgctatt
    23761 tctgatactg taggttcttt agaatttgtt ctaattaatc tttctctaac ttgtagtgcc
    23821 ttatatgcta tatcttttaa tgacctactt actctaattg ggttattgtc tcttagatat
    23881 cttctaattt ctcctataat cataggaaca gcataagttg aaaaccttac attttggctt
    23941 aaatcaaagt tatctatagc ttttataaga ccaatacaac ctatttgaaa taaatcgtct
    24001 atattttctc ctctgttgtt aaactttttt attacactca agactaatct taaatttcct
    24061 cttacaaact gttgtctggc ttcttcatca ccatttttta tttgtaataa aagttccttc
    24121 atttgcttat ttttaagaac tggaagttct gatgtattaa caccacatat ttcaacctta
    24181 ttaacttgca tatattttca gtcctctctc aaaagtattc atctattgaa attatttaca
    24241 tttccaattt ttttatactt tttaagaaga attaatttaa cattatacaa attttttcat
    24301 ttctttttgc aaccttgaga ttattttctt ttcaagtcta gatatgtagg actgtgatat
    24361 tccaagcatt ccagcaactt ctttttgtgt tttttcaata cctttatcaa taagaccaaa
    24421 tcttaattcc ataatttgct tttctctgtc acttaatctg tcaagtgcca taaccaacaa
    24481 atctctatct atttcttctt caattatttt gtatatttca tcattttctg tacccaatac
    24541 atcagataat aaaagttcat ttccatctaa atctatattt aaaggttcat caaatgatac
    24601 ttctgttttc tttttattat tttttcttaa gtacattaat atttcatttt ctatacatct
    24661 cgaagcataa gttgccaatt ttatattttt attcagttta aaagtattaa ctgcttttat
    24721 caaacctata gttcctattg aaatcaaatc ttctacatct atacctgtat tttcaaattt
    24781 tctcgatatg tagactacta atctaagatt tctttctatt aaaattgact ttacactttc
    24841 atctgtttct agcttttgta aaagctccat ttcttcttct ggtttcaatg gaggaggaag
    24901 tatatttgcc cctcccatat agtatattcc ctttggcttt attattttta atttaatacc
    24961 taacatagta ataaagctta ttattctttc tttcaatcgt aacattcctt gtccccctta
    25021 aattttcacc taaaatccag gcaatatatt tggattcatt attgctccat attctctatc
    25081 attaaaattt gaaataccaa gtattatatt tcctatcttt tgctcatcta cttctatata
    25141 gtcagctttt aagcctatta ttatgcttgt cttattactt ccagcatgtt tataaggtat
    25201 tattctaact ctacctgaag ttttatcatc taaacttcct ataatttctt gtgctttctt
    25261 catatctata tcttcataat taatatttaa tagtttttct ggaataaatc cttctaacac
    25321 actagactta actattatta catcactttg acttattggg tcttttaaca aattaccact
    25381 atctactaaa gctttacagc aaaaactttt ttctagtaag tttatattta tagtctttgt
    25441 aagctcctta atgtacttaa gcattttcaa atctttataa atgtatttta ataattcaca
    25501 actcacataa gtacatgcaa ttaagaaaga tatttttaag tgacttattc ctgtaaaata
    25561 aattataaaa taagtgcttc cagaaataaa aatgtttact agataaaaaa ctaacgctac
    25621 atgaacataa tcttttttgc tcttgcttct aaatgaaatt aaagttatta tgctcatgat
    25681 tattatttta aatggtacag taaatagtat attcattgat ggatacaaat aagcaacaga
    25741 ataggcactt cctaaactag cccccaataa cttatttttt ttaggattat atcttttagt
    25801 aagaatagaa gtacaactta taataatata atttattact aggttttcta taacatagta
    25861 ctcaatatac accatgtatc cccccttaaa acctttaaac atattataag atagtatgta
    25921 caaaatttta tgtcactttt gatggtcgaa tccaaaacaa attctattta aaaagaacaa
    25981 tgatatatgt cttatacttt atgtcgtcaa aagatatttt tttaatgcca aacagataga
    26041 cttttttgta gaatccaata tatctaggta gaagttattc gctttacttt tggtatattt
    26101 ataaaaaaaa gactacataa tttaataata tatcaagtaa atcttttact tatataaaat
    26161 caaattatat agtctgtaaa ttcaaatata aattattgtg ctagtaatcc atttgcatat
    26221 tttgcagatt catttagtgc ttgttctgga gttatgtctc cagctatcat catttgaagt
    26281 tgtttccata aatattcata tatctctact ccatgaggtc caactgtcaa tggtctatac
    26341 atatgtttat ctcttgtcac catttcttca aatcttgggt ctccttggta tttttcacct
    26401 ttagcaactg gagctctagg tatttctttg tggaaagctt ccattgattc tttgcttaac
    26461 atatactcaa gaagtttaaa tccaagttct ttatttttac atgctgacat taatgttaaa
    26521 tcatctactg aagcaaaact tccaactgtc ttatcttcta atgatgttac atatccccag
    26581 tttaaatccg ggaatgattt atcaaatgta cttgtagcag cagaggatag cattatagta
    26641 aatgcagctt ttcctggtcc aaatacactt tcaatcattt cattactatc ttttgataaa
    26701 gcatcttctg gaatatactt tttcatatca tttataaagt ttgcagcttt caagccttct
    26761 gcattattaa atttaactga ttttaaatca tcattatata tatctccacc agcttgccat
    26821 aaatacggat accagttcca gtttaagctt ccaaatactt tagaacccca accttgtgca
    26881 agaccccact ggtcgatttt tccatctcca tcagtatctt ttgttgcttt ttcacagatt
    26941 cttttaaaat catcccatgt ttttggaggt ttttctccta atttttctag tatatcttta
    27001 ttgtagtaca atacgcctgg attagcagct tctataggta aaccataaat accacccatc
    27061 atttttgcat catctaagta tagataatta tctgaagttc ctgatttttc taaataaggt
    27121 gttaaatctt ctactgcacc catttctata aattgaggga acatctctgc atacatgtat
    27181 cctatatctg gaccttcacc tgcacttatt gctgttgcat atttttctgc gtagttatcc
    27241 catggtacta tctgaaattc aatttcacag ttattttctt tttcaaattt ttcaaatatc
    27301 ggcttccatg catctgcatc atggtctgtt aatggtgcag accatacaac taatttttct
    27361 ttaccctctt tgcctttaga atcttctttt tttgaacatc ctgcaagaat tgatactgat
    27421 agaatcccta ccataactaa agaaagaacc ttttttagtt taatcatgct taatcccctt
    27481 tcagacttta atttgtcttt ttcctcaaat gtttacattt aattcagatt taatattaac
    27541 aaataagact actcttgtct aatcataaaa aactatacta actcctataa ctatattttt
    27601 tttctatacc tattagattt aattcatact aaaaacttac tttttaaatt ttatcaatat
    27661 ttaaaaatat ttttaagtct agccacaaac taactatcta aattgaacaa aataaaaaga
    27721 agtctattga cttctttata agcttacata ttatcttctt cttcttagga atgtaggtat
    27781 ctccatgctc tcatcatcat cttcttcaat tatacttgcc tttttaggag gctctatttt
    27841 ttcctcagca attacttctt cttcaatttc ttttacagct tgttttactg ttgtatttaa
    27901 tgaagaacgt atgcttgatt taggtttagt atctaaatct aaggcaccat tttgtaatcc
    27961 ttcaaatcct gttgcgatta ctgtaatcat tatttcatca cctaaatctt cttttataga
    28021 agctccaaat ataacatttg cttctgggtc acaggattcc ataactaaag ttgatgcctc
    28081 attaacttca aataatccta agttaggacc acctgtaaca ttaagaagta ctcctttagc
    28141 tccttgtatt gatgtttcta gaagtggaga ttgaatagct tgtcttgcag catcaattgc
    28201 tctagtttca ccactagcac tacctatacc catatgagca agtcccttgt ctttcattat
    28261 agtagtaaca tctgcaaagt ccaagtttat taacccttca actgctataa ggtctgaaat
    28321 tgattgtata ccttgtttta atacatcatc tgcaactgca aaagcatcta acattgatgt
    28381 atttttttgt actatctgta aaagtctatc atttggtatc gttataagag tatctacttt
    28441 tgatttaagt tctgctattc caccttcagc gttcttcatt cttatcttac cttcaaatgc
    28501 aaaaggtttg gtaactacac ctactgtaag tatccccatt tcttttgcca agcctgcaac
    28561 cactggagca gctcctgtac cagtaccacc gcccattccg gctgttacaa agaccatatc
    28621 agctccttga agtaacttta ctatttcatc tttactctcc tcagcagctc ttttccctac
    28681 ttctggattt gctcctgctc caagtcctct agttaatttt tctcctattt gaactttata
    28741 ttcagctttt gatgtatata aagcttgttt atctgtattt actgatataa attcaacgcc
    28801 ttttagttga gcttctacca ttctattgac agcattgttt ccaccgccac ctacacctat
    28861 tactttaatt tgtgcacatt cttctaattc tacgtcaaag tttagcatca taaaccccct
    28921 tctctcatga ttcaaagtta tcgtatgtat taattatatc attttcttct tttattttgt
    28981 taacctttaa gtaacatatt ccacaaaaac gtaaattttc ctttttaaat ttcttactta
    29041 tattgtcaaa tattactctt taagtataaa ataatttacg tatatattaa atgctcttta
    29101 atttttacgt tttttgttca taatattact aataatatat cgtctaatta tagataagtt
    29161 attaaacaat ctcataccaa aaactagtac agcaacatag taaagagaaa ctcctaactt
    29221 ttctcctata taagtcaata atatagccaa tatagcatta cctatgaaac cagtaataaa
    29281 aattacatta tcatagttat cttcaaaatt ggattttatc gctccaaaaa tagaatccat
    29341 tattgcaaga atagctactg acatatatat ggaataatcc atagaatatg taaatggaat
    29401 atagtaacct actactacac caaatgctaa tcctcctaaa acccacaaca taaatcttca
    29461 cctctttatt ttactttgaa acacaactac attacaatcc tttttattaa ttttttttac
    29521 tttttcttta tttttttgta tttttcgttt tattttgcaa ataaattcac tttattccca
    29581 ttattttcct gaacttttat attaattcca tatatatcct taagagattt agcatatgat
    29641 tctggagagt ttatagctgc actcaataaa tctatatctc ctatagcttt tattataaat
    29701 ggttgtccat atgtagtatc atttactgta atagtagctc ctgaacattt tatcttactt
    29761 gttgctagta ctctttcccc atttatagaa attgctcttg ctccagcctt ttttaaatca
    29821 tttaaaactc tcaaaatatc tatatcatgt ataatcaaat catttggatt ctgtccgtct
    29881 tttaactctc tctcactatc cctcatagta atagttatgc ctggccctgt aacagttgaa
    29941 taaccactta attctttaac ttcttcaagc tctttttgca taagtgtttg gatagattca
    30001 tttccattct tttttgcttc tttatattca ttcaaagttt ttttgttgtt tttaagagtt
    30061 tcttttaaat tttttatttc tttttttgta ttttctacct cactttcaat actttcagtt
    30121 tgacctaatg ttatgtatac tctatttggg tctagtgatt ttatataagt tgatatgaaa
    30181 atgcctaata tgaatgctcc taatataact attttctttt tcatactatt atttcccctc
    30241 tgctttgttt atatcactat gaccatttat ttctatattg tcttctttgg tcacttttac
    30301 tttggcatta taatatgttt ctatttccca gacaatacca tactttatat taagtgccga
    30361 agccaatgta tcagaatcac caattgcttt tattataatt ggttctttta tgacagtgtc
    30421 attcatacaa agcttgtctt cttttaaata catatatgta ttaaaaacta ttctctggtc
    30481 gtttatagat atggcacttg cttgtgctga atttaattta tttatcattg acaataataa
    30541 atcataatta tatagtatac tatcattttt atcagagttt cctaagtctt ttgtagaatt
    30601 tatctcaatt tttatgcctg ttccttcaac atctgtatat ccagctaatg tttcatattt
    30661 ttctatttct gacttaagta tgtcttgttc tcctttattg tttttgcctc tataagtctc
    30721 tatatctttc ttcacactat taacttctga ttgtagattc tctttttctg tttttaaaga
    30781 tttaagttct tctgtaagtt gctctcctct tttaaaagtt gtcatccctc tattttctat
    30841 attaataatt ttaagttgta aactaacaag aacacccaat acaacacttc ctatgattat
    30901 acttttattt ataaatcttt ttttcaatat atcacttcct tacaattgtc tatccttctg
    30961 gactatacac tgccaacttt ccatagttaa gatttatagt ccctccatta gtatttttat
    31021 tttgcaggtc aatcagaatt ttacttaccc tagatatatt ataatctaag ttatcatcat
    31081 tagaaagtaa gatttttata ttacttcttg taagcatttc tatattgctt tcttttttta
    31141 agtttacata atttattttt ctataaattc cttcttcttt taaataattt aaagttttaa
    31201 atacattctc cttagtttta taattt
//
LOCUS       DAEPOG010000024        30759 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEPOG010000024 DAEPOG010000000
VERSION     DAEPOG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.024"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..696
                     /locus_tag="KR432_000990"
     CDS             <1..696
                     /locus_tag="KR432_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0639541.1"
                     /translation="IILIILSILSMLFIFVFVIPNFQVVFTNNGIEPPLITRVLMGIS
                     TVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPFIKKINQLVATTRFS
                     RALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGNSIGKSLNLANVFPK
                     LFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPMLIVFMGLIIGTFIL
                     AMVVPMFDAITNF"
     gene            750..1109
                     /locus_tag="KR432_000991"
     CDS             750..1109
                     /locus_tag="KR432_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBH0639542.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            1162..2034
                     /locus_tag="KR432_000992"
     CDS             1162..2034
                     /locus_tag="KR432_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH0639543.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            2035..2478
                     /locus_tag="KR432_000993"
     CDS             2035..2478
                     /locus_tag="KR432_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639544.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            2493..3077
                     /locus_tag="KR432_000994"
     CDS             2493..3077
                     /locus_tag="KR432_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639545.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            3330..4367
                     /locus_tag="KR432_000995"
     CDS             3330..4367
                     /locus_tag="KR432_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0639546.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            4448..4813
                     /locus_tag="KR432_000996"
     CDS             4448..4813
                     /locus_tag="KR432_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639547.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            4818..5135
                     /locus_tag="KR432_000997"
     CDS             4818..5135
                     /locus_tag="KR432_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639548.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            5183..6787
                     /locus_tag="KR432_000998"
     CDS             5183..6787
                     /locus_tag="KR432_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639549.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            6911..8539
                     /locus_tag="KR432_000999"
     CDS             6911..8539
                     /locus_tag="KR432_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0639550.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            8804..10153
                     /locus_tag="KR432_001000"
     CDS             8804..10153
                     /locus_tag="KR432_001000"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH0639551.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            10309..11388
                     /locus_tag="KR432_001001"
     CDS             10309..11388
                     /locus_tag="KR432_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0639552.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            11733..12641
                     /locus_tag="KR432_001002"
     CDS             11733..12641
                     /locus_tag="KR432_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0639553.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            12646..15015
                     /locus_tag="KR432_001003"
     CDS             12646..15015
                     /locus_tag="KR432_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH0639554.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            15340..16143
                     /gene="proC"
                     /locus_tag="KR432_001004"
     CDS             15340..16143
                     /gene="proC"
                     /locus_tag="KR432_001004"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0639555.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            16741..19497
                     /locus_tag="KR432_001005"
     CDS             16741..19497
                     /locus_tag="KR432_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639556.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            19629..20108
                     /locus_tag="KR432_001006"
     CDS             19629..20108
                     /locus_tag="KR432_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0639557.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            20124..20546
                     /locus_tag="KR432_001007"
     CDS             20124..20546
                     /locus_tag="KR432_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBH0639558.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            20586..21377
                     /locus_tag="KR432_001008"
     CDS             20586..21377
                     /locus_tag="KR432_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0639559.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            21370..22170
                     /locus_tag="KR432_001009"
     CDS             21370..22170
                     /locus_tag="KR432_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0639560.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            22218..23273
                     /locus_tag="KR432_001010"
     CDS             22218..23273
                     /locus_tag="KR432_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0639561.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(23433..25379)
                     /gene="feoB"
                     /locus_tag="KR432_001011"
     CDS             complement(23433..25379)
                     /gene="feoB"
                     /locus_tag="KR432_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0639562.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(25397..25615)
                     /locus_tag="KR432_001012"
     CDS             complement(25397..25615)
                     /locus_tag="KR432_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0639563.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            25821..26330
                     /locus_tag="KR432_001013"
     CDS             25821..26330
                     /locus_tag="KR432_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBH0639564.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            26408..26569
                     /locus_tag="KR432_001014"
     CDS             26408..26569
                     /locus_tag="KR432_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBH0639565.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            26720..27385
                     /locus_tag="KR432_001015"
     CDS             26720..27385
                     /locus_tag="KR432_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0639566.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            27451..29235
                     /gene="pepF"
                     /locus_tag="KR432_001016"
     CDS             27451..29235
                     /gene="pepF"
                     /locus_tag="KR432_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0639567.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            29487..30329
                     /locus_tag="KR432_001017"
     CDS             29487..30329
                     /locus_tag="KR432_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0639568.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      30562..30645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT        12357 a         3280 c         5412 g         9710 t
ORIGIN      
        1 ataatactta taatattatc tatcttatca atgttgttta tattcgtatt tgtaatacct
       61 aattttcagg tggtttttac caacaatgga attgaaccac ctttaataac tagagtttta
      121 atgggaatat ctacagtggt tacaaataac ttagcttata taatatttag ttttatattg
      181 ttttcaatag gaagcattta tttctttata acaaatgata atataaaaaa attgattaat
      241 tcgttaaaat tcaaaatacc ttttataaaa aaaattaatc agttagtagc tacaacaaga
      301 ttttcaaggg cattgtccgt tttaattagc agtggaatac aaattgtgga agctatagac
      361 atgtcagcta gtgttataga caacgaattt atttatgaaa ggttaaagat ttctaataat
      421 tatataaaaa aaggaaatag cataggtaaa tctttgaatt tagcaaatgt ttttccaaaa
      481 ttatttattt caatggcaaa tacaggagaa gaaagtggga aactagataa aagtctaaat
      541 gtaataaata aattttatga aaatgaatta aataacaaaa tagagcaaat aatgaaattt
      601 atagaaccta tgctaattgt atttatggga ttaattattg gcacttttat tttagcaatg
      661 gttgttccta tgtttgatgc aattactaat ttttagagag aaaaataaat tttattagat
      721 gaaacttttg aaaaggagag aatatttaat tgataaattt gataaataaa aaacgaaaag
      781 gatttacact tgttgaaatg attgtagtag taactatttt aggcgttata tctagtatag
      841 cattagttaa gtatagtaag gttcaagaaa gtgccaaatt aaatgcagac tatacgaatg
      901 ctgctaatat agtaactgca gctagcatgg caattaatga tgatgaaaag acaatagact
      961 ctctaagtgt agaaacattg aaggaaaagg gatacctaaa tactgttcca gttcctcaga
     1021 gtacatcagg taaattcgaa cttgtcataa atgatagcgg aacagatata agcgtaaata
     1081 taaattctaa acaattttat ccaaaataat attttatatt gattgctgta agttttaaaa
     1141 ttatttttat ttaagaagga gatggctata attaaaaaaa ttctaaactt agaaataaat
     1201 gatgattata taaggcttgt atttttaaga aaaagtcata aaaaagtttt tatagataaa
     1261 tcaattataa aagagattaa ctttgatata aaaaatgata gtaattcgat tgagaagatt
     1321 aaatatatag aagatgtagt tagatttgta aaagaggaga ttggtaaatc aaaaatatta
     1381 tataaaaatt tatactttaa cattcaaatt caagatatag tcattagaaa tgttgaagtt
     1441 ctaaatacta agaaaaaaag agacatatta agcatgattg aatttgaaat aacacaatac
     1501 attccaataa acataaataa ctattcaata aaatataaag ttataaattc taaaaatgat
     1561 aaattgaata tacaagtaat tcttttccca aagagtatta ttcaattaat taagaacatt
     1621 tcagaaaatc taaatatgaa tccaaagact ataaatataa attttgatat attacaaaag
     1681 cttataaact taaatttaat agagaatttt tcagaagaag gggtatttat agaatgtaaa
     1741 aaaagtcagt taattataaa tataacaaaa gataaaaaaa taagagaaac atacttgcta
     1801 tcaaaagagg gaaaatcata tgagttcata aaaagattat taagggattt taagtatgtt
     1861 tattactatg gtatagaaaa ttgttttatt gaagaatact ttaaatatac agataaatct
     1921 attgaagtaa atccattaag attaaaagat aaagtaaaag tagaaaataa ttttgatgca
     1981 gatttagaag acaataggat aaattatata aatagtatag gaatgattat ttaagtgaaa
     2041 gtggataaaa caataaattt tcttaatgga tttaaaaaaa ctaaaccatg taataaaatc
     2101 tataaaataa taatagtctt aattttaacg tgtattttat ttaaatcatt tatgtatgtg
     2161 aaatctataa atgaacttga agattatata aatcaaaata agctagtgga taaatcaaag
     2221 cacactttac caacaacaga taaaactaga gagaaatttt ctaaaataaa acttaatgaa
     2281 ataaaaaaaa tatatgaaac aattggaata aatagtatta ctaaaatatc gatagataaa
     2341 aataagctag agatggaagg tgtgtgtagt gatttagatg ttttaaacaa cctaaggaaa
     2401 ataaagtaca taaaatatta ttacatagac agtattgtaa gagatggaga atattataga
     2461 tttagaataa aaagttagat aggtgattat ttttgagatt aaataataga gaaaaaaatc
     2521 tacttaaaat attgttaata ttagttatta tattggcaag tgatatagca gtaagttatt
     2581 tggaagaaaa cctaactagt ttgaagaatg aaaagcaaaa tataataaat aaaataaata
     2641 atcaagataa ttatcaaata aaggataata agcatataga tgataaatat atagaaagtg
     2701 ttgaattaaa taaaaatgaa gaattgaaaa ataattttaa taactcattt ttagataaaa
     2761 aaacaaataa agatatagtg ttattagtag aaaatgaact gagagatatt gtgaatgtag
     2821 aatatattaa tcaagaaagt agtttagata atcacaataa aaaacaagtt aatttagaaa
     2881 taaaagttaa tggagaactt gcaaatgtgt tgaaacttga aggtgtaatg aagaagttaa
     2941 gactaaataa taaacttgaa aagatagaaa ttaacaaatt aaaagtcaat aattttgatg
     3001 ttcaaaatat tccaaatgaa tataaacaag aaaaagacaa tattgttgaa tgtaaaatga
     3061 cagttagagt catataacaa aaaagaatag ataaagtaaa aaactattat caattaataa
     3121 aattttatag aggaattagt tttacgataa ttcatttcta caagagggat tgttgatgat
     3181 tttatcgaaa tcctaattaa agttaatata ttgtaccaaa atataagtga atttaataga
     3241 atatttactt atactataga ttgattaata aaaaaatggt ataataacaa attgtacttt
     3301 aatttaataa taagggagtg gaagtcatat tgttagggag aaatgagcta tgcccttgtg
     3361 gaagtggcaa aaaatacaag aaatgttgtt taaataaaga tattgtttca gaaagagcat
     3421 gcagaaaaac tgaactttca caaaaacaat atacagattt atattcaaag ttatatgatt
     3481 attcaagaca ggataagttt aaagaagaat gtgaaaaagc taaagaaatg ttttatataa
     3541 tgcaaaatga tagtgtaaat gaaaaatttg aaagattttt taatacttat ttaatacaag
     3601 accacataat ggaaaataaa aaggttatga cagttggttt tcttgaagaa aatggtgcaa
     3661 acctaagtca atcagaagta tcaatattaa aaagtatgtt tgaatcatac gtaagtattt
     3721 atgaggtaaa agaagtttct gctggaaaaa tagtattaaa ggactgttta agtggaaatg
     3781 aactttgtac agaagatgta aaacttctga gaagttttaa aatcggaagt tgtatgatag
     3841 ctagaatagt tgatattgaa ggaactaaca tattgataga tataactata agtatatcaa
     3901 atgaggttaa agatataatt ttaaaggata ttatgaatct attcaatcaa tatcaagatg
     3961 tatacaaaga tatgaagtcg ttcttaattt atcatacaca tatactatac aagtatatac
     4021 aacagctttt agacccaagt atagctgaat atttaaagag agaaagagat aaaaacgata
     4081 caaaagttaa ttcagaaaat gaattagctg aagaagaatg taaagtttta gatactctta
     4141 aagaaaatgt agaaaaagaa aatatagatg catgtataga gttttggaat gaatataaga
     4201 aaaatcatga aaatataaaa ggagctgaaa atggttgggc agctgctgtt gaatactatg
     4261 ttaaaaaaga agcaggacaa cctataactc aagttcaaat atcaaagaaa tacgatataa
     4321 gctctagtac actaggaaag agatacaaag atttaaaaat atcttaatta attataaaaa
     4381 gctttaagtc atagataaag aatctatagt ataatatatg tataatgttt agaaagaggt
     4441 attaataatg agtcttagtt taggaaatgc aattgctgta tcaacgaact ttataaaatc
     4501 taacccagct gtacctgtgt acaacggaga agaaatatat gcagttattt atcatataga
     4561 aataggatat aataatggtg tatcacctga taccaaacaa tatataataa tagactataa
     4621 agagtttgat ttatctatgg ataaaaaaaa tgaactaata aatgttgcta aagagtactg
     4681 taaatctaaa ggtattgtag aaaacgaaaa ccttgaaata aaccttttag acaatgaaga
     4741 agcagagaac ttcttagaaa aggtgtttaa taacatgaag gttataagag gtcttataac
     4801 taaaaaagga taaataaatg gactatatag gaatagaaaa tataacacct tatgaaaata
     4861 catatgaatt tagtgtatat gaatatgatg atgaaatcac cttaggtagt gaaaagttat
     4921 atgtgtgtga attaagggtt gtattgatta aagttaattc tctgtatgtt gaaagattgc
     4981 ataaatcagt tgaagcaatg gtcttagtaa aaaacttgaa aaaggattta gataaaacac
     5041 ttgttgtaaa caaaataaag aattttgtgc tagatgagat ttgggtagaa aatctagtaa
     5101 aagagaatat agaagttata tttgtagaaa gctaggaata accctagctt ttttatatta
     5161 caatctaaga gaggaaaaca atatgttaag agtatctaat gtaaaacttg gaattgataa
     5221 agatatatct ttattaaaag atgttgtact taagaagcta aaaataaaag aaaaaaactt
     5281 agtcaaatat agtatatata aagaatctat agatgctaga aaaaaaggaa aaatggaatt
     5341 tgtttattct gtagatgtag aagtcaaaga tgaaaataaa atacttaact caaaaataaa
     5401 agatgtaaca aaaataaagg aaatcagata cataaatgta cctgtgggtg ataaaaaatt
     5461 aaagaataat cctttggtaa ttggtagtgg accagctggt ttatttgcag gtctgctttt
     5521 agctcaaatg ggttatgaac caatcatact tgaaagaggt cttgatgtag acagtagaac
     5581 caaagatata agtgattttt ggacaactag aaaattaaaa aataattcaa acgttcaatt
     5641 tggagaaggt ggagcaggaa ctttttcgga tggtaaactt acaactagaa taaaagatat
     5701 acgatgtaga aaagtattag aagagttagt tcagtttgga gcaccagatg aaatattata
     5761 ctcacataag ccacatgttg gaacagatat attaaaaaat gtagttaaaa atataagaaa
     5821 tgaaataata agtttaggtg gtcaagttag atttaattca aaagtaacag atatagttgt
     5881 atcaaatgat tctatacaat ctgtaataat aaatgatact gaaaaaatag atacagatac
     5941 aattatacta gctgttggac atagtgcaag agatacttat gaaatgttgt attcaagagg
     6001 tataaaaata gttcaaaaac cttttgcaat aggagctaga gttgaacatc cacaagattt
     6061 aataaataaa gcacagtaca aggattttta taatcatcct aggttaggag ctgcagatta
     6121 tagacttata gagcatgttt caaatggaag aacagcatat actttttgta tgtgtccagg
     6181 aggtagtgtc atagcatctg catcaaatga ttttgaaata gttacaaatg gtatgagtga
     6241 acatgcaaga gataaaataa atgcaaatag tgcattttta gtaaatgtaa cgccagaaga
     6301 ttttaagagt gataatccac tagcaggtgt tcatttccag cagaagtatg aaaaattagc
     6361 ttttgaatta ggtggaaaaa attacaatgc accagttcag cttgttggag attttataaa
     6421 caataagact tctttgaatt taggaagtgt tgagccatca tatagaccag gatatacact
     6481 tacaaattta agtgaatgtt tgcctagctt cgtaacagag actatgagag aagcgtttat
     6541 atctcttgat aaaaaattga atgggtttgc aatgtatgat gctgttttga cgggagtaga
     6601 aactaggtca tcatcaccaa taagaatagt aagagatgaa aatactttag aatcagtgag
     6661 tattaagaat ctatatccat gtggtgaagg cgctggatat gcaggtggta tagttacagc
     6721 agcagttgat ggtataaaat gtgcagaaaa aataatacaa aaatacacca ataatacaaa
     6781 aatttaacat aaacttaatg attttttaat atttaacatg aatttaacat tatcactcct
     6841 ataatttgat aaaatacaat aggttataaa atttttagat acagtaatct ctttacaagg
     6901 aggaaataat ttgaacatta taataagtct aatgggtgga ttaggtctgt tcttatatgg
     6961 tatgaacctt atgggagaag gtcttcaaaa atctgctggg acaaagctaa aaaagataat
     7021 aaaactactt actagtaacc tatttatggg agttttggta ggtacaggag ttacagcagt
     7081 aatacaaagt tcaagtgcta ctactgtaat ggttgttggt ttcgtaaatg caggaataat
     7141 gactttaaaa caggcaatag gagttataat gggtgctaat atagggacta ctgttacagc
     7201 ccaattggta tctttcgact taactggtat ggccccagta gcattaggtg taggtattat
     7261 actttattta tttggaaata agcctagaat aaaaaatata gccgaaatac ttataggttt
     7321 tggtatatta tttacaggta tggattttat gaagatggca gtggagccac taagagacta
     7381 tcaaggattt actgacctac ttgttacatt tggtagatat ccactacttg gattattatt
     7441 gggatttggg attacagcaa taatacaaag ttcaagtgca tctatgggta tgttggttgc
     7501 actggcagca gaaggtctag tgccattatc agcagcactg ccaattcttt atggccaaaa
     7561 tattggtaca tgtgtaactt cactgttatc aagtattggt gctaacaaaa atgcacgaag
     7621 agcagctatg atgcatctaa tcttcaatgt tttaggaacg gtgatattct taatattctt
     7681 gaataaacca gtagtaagca tggttacaag ttgggatcca agcaatgttg caagacaaat
     7741 agccaataca catacattgt ttaatataat atctgtattg atacttttac catttacaaa
     7801 tcttattata aaactagcta taaagcttgt tcctgataga gcaggtgata tagatgaaga
     7861 tgaaactaaa actattaaat atatagatga tagaatgata gaaactcctt ctatagctct
     7921 tgcaaatacg ataaaagaag cacttcgtat gggtgagaag gcgaaggaaa gcctaaatgc
     7981 atctatggaa gccttagtag aacattcaac tgaaaaaata gctaagacat atagacgaga
     8041 gagattaatc aatgatttac agaaagctat tttaaattac ttacttaaac tttcaaaagc
     8101 acctttagat gatgattcaa gagaagttgt ggatactttg tttaatacag tcaatgatat
     8161 agaaagaatt ggtgaccacg ctaaaaatat agctgaatta tcgcaagttg caatagattc
     8221 aaatatttct ttctcagaag aaggacaatc tgaacttgat gttatgtata atagagttgt
     8281 ttcagcatat acttatgcat tagaatcaat gagaactgat aatgttgatt tagcttgtaa
     8341 ggttataaaa attgaagaac aagttgatat aatggagaaa tcttgtagag ctaatcacat
     8401 gtatagattg aacaataatc tatgtagtat agaaaatgga gttatttatt tagacgtaat
     8461 aagtaattta gagagaatat ctgaccatgc tgtcaatata gcacaacaag ttatagcgaa
     8521 gagattagga aatgattaaa aataattaat ttaaaataat ataaaattta aaatagagga
     8581 taaataaaag agaaacttta aatgatacgt ttagagtttc tttttttgta aatttatttg
     8641 actaaaattt ttaaatttat acatagttat agtatttaga agaatcgatt tcctgataat
     8701 aaatgatgag atatgtatga ttttgtggca aacttttaaa aaaaacacct atattttgtg
     8761 ttataatctc tttaaaacga atatgctgaa aagagggatt taaatgggaa aaataaattt
     8821 tgattatagt aaagctacag agtttttttg tcaaaacgaa atagatgtca tgcaaccata
     8881 tgtagatgtt gcgcacgaca tgttacataa taaaactggt ttaggaaata cttttttagg
     8941 atggatagac cttcctaaaa attatgataa agaagagttt gacagaataa aaaaatctgc
     9001 tgagaagata aaatcagact cagatgttct tctggtaata ggtataggag gctcttattt
     9061 aggttcgaga gctgctatag atatggtaag tcattctttt aggaatggac ttaaaaaaga
     9121 gcaaagaaag gctccagaag tatattttgt agggcataat ataagctcaa catatattat
     9181 ggatttatta gatataattg aaggaaagga tatttctgta aatgtaatat ctaaatcagg
     9241 aactactaca gaaccagctc tggcttttag aatatttaaa gattacttag agaaaaagta
     9301 tggtaaggaa gaagcaagaa agagaattta tgcaacaaca gatgcaagta aaggtgcact
     9361 tagacagtta gctacagaag aaggatatga gacttttgta ataccagatg atgtaggtgg
     9421 tcgtttttct gtattgacag cagttgggtt acttcctatt gctgctgctg gacttgatat
     9481 tgatgctatg atgaaaggcg caaatgatgc tagagaagca tttcaaaatc ctgacttaaa
     9541 aagtaatgat agttatagat atgctgttgc tagaacaatt ttacatagaa aaggaaaaga
     9601 tgtagagtta ttagtgaact atgaacctca actacattat gtaagtgagt ggtggaaaca
     9661 gctttatgga gaaagtgaag gaaaagaaaa taaaggttta ttccctgctt ctgtagattt
     9721 ctcaacagat ttacattcta tgggtcaata catacaagat ggaaaaaggc ttttatttga
     9781 gacagtactt aatgttgaaa actgtaagag aaatataact ataagttcag aggaagtaga
     9841 cttagatggt ttaaattatt tagcaggtaa gactgtagat tttgtaaatc ataaagcttt
     9901 tgaaggaact ctattggctc atacagatgg aaaagtacct aatttagtaa taaatatacc
     9961 tcaactagat gagtataatt ttggatattt agtttatttc tttgagaagg cttgtggaat
    10021 aagtggatat ttattgggag ttaatccatt tgaccaacca ggagtagaag cttacaagaa
    10081 aaatatgttt gctttactag gcaaaccagg ttatgaaaaa gaaaaggaag agctagaaaa
    10141 aagacttaaa taattttaaa tagttaaggt ttaattaaga ttaaaataat ataatgaaat
    10201 catcgaaaag gagtttactc aaaatagatg atttcatttt ttttatgaaa aattgggaaa
    10261 aatatatttt gataatttat attatattaa gatagggagt ggtatgagat gtcaagaaga
    10321 aagaaaggga taagtttagt aatattagtt gctataataa gttcaatact tagtagtttt
    10381 ctaacaataa ttttagttaa ggacaattta gtaagcaaat ctacaggaag ttctacgccg
    10441 atagttgtaa atgatgatgg taagagtcaa aatatttatc aagcagttgc agaaaaagct
    10501 acgccatctg ttgtaggtat tacaacaact agtgttgata ctagtaatat gtttgcaata
    10561 ccaactgaaa cacaaggtgt aggaactgga atcatagttg attcaaatgg atatatactt
    10621 actaattctc atgttatatc agatggtcaa gctacaagtg taaatgtatt attcaatgat
    10681 ggttcaacaa cttcaggtaa agtagtatgg tttgaccaac aacttgattt agctatagtt
    10741 aaggttgata aaactggact aacaccagca gaatttgctg atagtgacaa agtaaaagtt
    10801 ggagatatat caatagcaat aggtaatcct cttggattag attttcaaaa aacagttact
    10861 caagggataa taagtggatt agatagaact atacaaacag aaaaaactaa tatgactggt
    10921 ttattacaaa ctgatgcaag tataaatgca ggaaatagtg gaggaccatt gttaaatcaa
    10981 aaaggtcaag taattggtat aaacacagca aaagcttctc aagcagaagg attaggattt
    11041 gcaataccaa tcaatacagc taagagtata gttgaagaag taatcaaaaa tggaaaatat
    11101 gaaaaagtaa ctttaggcat aaaaggtaca gatgtatcaa attatgaagc agcaacaggt
    11161 acaaaattaa gtactgataa aggtgtttat gttgcagaag taatatctgg ttcatcagca
    11221 gaaaaagctg gagtaaaggt aggggatatt ataactaaag taggagatac agatataaca
    11281 ggtatgaatg atttaaataa aaagttgtat actttctcaa aaggtgctag tacaaaaata
    11341 acagtaaata gaggcggaaa agcagtaaca ataaatgtga atttctaaat atgaccagaa
    11401 agaaggagac tacatggtaa agtgtagtct ctatttttat ttaattttta tagtatatac
    11461 atctaaatta attaaaatga tgtattgttt atagtttatt gctatatttt cttataaaaa
    11521 taaatttaat aaatatttaa atttaattta caaaaacaat ataaatatca taataaaata
    11581 aaatttttta atattactaa aaatatatag acaaaataat aacaaatata gtattatgta
    11641 attgtagaaa tgcttttcat aggtatattc tatgaagatg aattgcataa aaatgaaaaa
    11701 atggaatgga tattatttag gggtgaaaaa tcatgaatcc attagttata aacttacaaa
    11761 aatgtagcat acatgatgga ccagggataa gaagtacagt attttttaaa gggtgtccac
    11821 ttgaatgtgt ttggtgtcat aatccagaga gtcaaacata tacaaaacaa gttttgtata
    11881 atgaagaaag atgttcaaaa tgtgaagcat gtataaatat atgccctcat aaggcaattt
    11941 acaaaggtga aacaaaaata tgcttagacc aagataagtg tgagttttgt gaaacttgct
    12001 tagactattg tgtaaacaat gcaagagaga ttgttggtca agagtattct gtaagagact
    12061 tggttaaaga gatagagaag gatagaattt tctatgaaga atcgggtggt ggagtaacac
    12121 tatcaggtgg agaagtaatg gctcaagata tggacttcat ttgtggagta ataaacatgt
    12181 gcaaatcaaa gggaatccat gtagcaattg atacatgtgg ctatgcaaag tcagaaaatt
    12241 atgaaagagt tgctaaatgt gcagatttgt ttttatatga tattaagctt attgatgagg
    12301 ataagcacat aaaatttaca ggaaaatcaa atgacttaat actgaaaaat gttaagattt
    12361 taagtgaatt aggggttaac ataaatataa gaataccatt aattgtagga gtaaatgtag
    12421 atgatgaaaa tttagaggtt aaaaaaatga tagagttttt aaagcctttg aatatacaag
    12481 cagtaagtct tcttccatat cataatattg gtaaacacaa gtatgataaa atttataaaa
    12541 agtatgaagg agaagaacta caaagaccat cagaagaaaa attagaggaa ataaaaagac
    12601 tgtttgaagc aagtaatttc aatactaaga ttggagggta ataaaatggc aagaggaact
    12661 tttgagagaa ctaaaaaatt aagagaagaa agtattaatg cagaaccaca catatctatt
    12721 gaaagagcag tacttatgac agaagcttat aaaaaatatg agggaagtgt agagattcct
    12781 gtacttcgtg ctttatcatt taagcattat attgagaata gaactcttag cataaatgat
    12841 ggagaattaa tagtaggtga aaaaggtgat tctcctaatg gagcaccaac atatccagaa
    12901 atttgttgtc acacaatgga agatttagaa gtaatgcaca atagagatat aataaatttt
    12961 agcgtatcag aagaagctag aaaaatacat aaagaagaaa taattccttt ttggaaaaaa
    13021 agacaaacaa gagataagat aataaatgca atgacacctg agtggttagc agcatatgaa
    13081 gcaggtatgt ttacagagtt tatggaacaa agagccccag gacatacagt ttgtggagat
    13141 acaatatata aaaaaggatt tttagattta aagaaagaca tagaagcaag attaaaagag
    13201 ctagattttc ttaatgattt agatgcatat aataaaaaag cagatttaga agctatggca
    13261 atagcttgtg atgcaatggt tatattaggt aaaagatatg ctgagaaagc aagacaaatg
    13321 gcagaagaag aaacagatga agctaagaaa aaagatttat tactaattgc tgaaacttgt
    13381 gatgttgtac cagctcacaa accagaaact tatcatcaag ctatacaaat gtattggttt
    13441 gttcatatag gggttactac tgaacttaat atatgggatg cttttactcc aggaagactt
    13501 gaccaacatc taaatccttt ctatgaaaga gatgtagaaa atggaatact agatagagat
    13561 agagcacaag aattacttga atgtttatgg gttaaattta acaatcagcc agcaccacca
    13621 aaagttggta taacattaaa agaaagtagc acatatacag attttgcaaa tataaacact
    13681 ggtggaataa atccagatgg tcaagatggt gttaatgaag ttagttatat aatacttgat
    13741 gttatggatg aaatgaaatt gatacaacct agttcaaatg ttcaaataag taaaaagact
    13801 cctcaaaaat tcttaaagag agcttgtgaa atatcaagaa aaggatgggg tcaaccagca
    13861 ttttataata ctgaagcaat agttcaagag cttatggaag ctggaaaaac tatagaagat
    13921 gcaagacttg gtggaaccag tggttgtgtt gaaactggat gttttggtaa agaagcatat
    13981 gttctaactg gatatatgaa cattccaaaa atacttgaat taacattaaa taatggatat
    14041 gacccaatta gtaaaaaaca aataggtatt gaaactggag accctagaaa tttccaatct
    14101 tatgaggaac tatttgaagc atttaaaaag caattacatt atatgattga cataaaaata
    14161 gaaggaaatg ctgtgataga aaatatatgt gcaaaacaca tgccttgtcc acttatgtct
    14221 acaatagttg atgattgtat agaaaaaggc aaggattacc aaagaggtgg agcaagatac
    14281 aacactagat atatacaagg tgttggtata ggaactataa cagatagttt aacagcaata
    14341 aaatataatg tatttgataa gaaaaaattc gatatggata cattgcttaa agcacttgat
    14401 gctaattttg aaggatatga agctatactt aatttagttt caaataagac tcctaaatat
    14461 ggaaatgatg atgattatgc tgatgaaata atgcaagaga tattcaatgc atattacaat
    14521 gaggtaactg gtagactaac tgtttgtggt ggagaataca gagtagatat gttaccaaca
    14581 acttgtcata tatactttgg agaaattatg ggagcaagtc caaatggtag actttgtgca
    14641 aaaccagttt cagagggaat ttcacctgaa aaaggtggag atactaatgg acctacagca
    14701 gttataaaat cttgtgctaa gatggaccac ataaaaactg gtggaacatt attaaatcaa
    14761 agatttgcac catcagtagt tcaaggagaa aaagggttag ataatatggc taatctagta
    14821 agagcatact ttaatatgga tggtcatcat atacaattta atgtatttga taaaaatgta
    14881 ctgttagagg ctcaaaagaa tcctaaggat tataaagact taatagttag agttgcagga
    14941 tatagtgacc atttcaataa tttaagtaga acactacaag atgagataat aggaagaact
    15001 gagcaaacat tctaggtttg cttgtaaata aaacttctta ctatataaaa tcgtaactga
    15061 tataataaag tataaatggt ttgattccat ttttaaaatt ttagattaat tatgagctaa
    15121 ctttctaaat aggaagttag ctcgttaagt ttaatgaaaa aatcgttaaa tttatattta
    15181 gtaaatcata cacatattag taagtaatac atacaaaaga ataatttatt atagttataa
    15241 taaggttata ttttataaaa ataagataat tttaagcagt taaaataaag tcaatttagc
    15301 tactttaaaa taaaactaat agaaaaaagg agatttgata tgaaaacttt aggatttatt
    15361 ggttcaggga atatgggaag tgcaatgata ggtggcatag tacagtcagg attagtaaag
    15421 gcagaagata taactgtatc agatttaagc caagttgcct tagataaagt aaaagaagaa
    15481 tttggaataa atactacaac tgattcaaag gtagctgtta caaattctga tgtggtaata
    15541 gtagcactta aacctaatat atgcgaaaag gctttaactc cacttaaaga attaatagat
    15601 gcaaataaaa taatagtatc tatagcagca ggtaaaacta tagaaagtct ggaaagtttt
    15661 ataggttcag ataaaaaaat agtaagagta atgcctaata ctccagccct tgttggcgag
    15721 ggaatgtcag ctctttgtaa aaatagtaat gtaacagatg aagagttaaa tatgattaag
    15781 gctctgtttg aatcttttgg tgaggcagaa atagtatcag aatatttaat ggatacagtt
    15841 acaggtatta gtggttcttc tcctgcatat gtatttatgt ttatagaagc tatggcagat
    15901 gcagcagtat tagctggaat gccaagacaa caagcttata aatttgcatc tcaagcagta
    15961 atggggtcag ctaagatggt tcttgaaact ggaaaacatc caggtgaatt aaaagatatg
    16021 gtttgttctc cagcaggaac tactatagaa gctgttaagg tattagaaga agaaggcttt
    16081 agagcagcag ttataaaatc tattgttgct tgtatagata agtctaaaga tatgagcaaa
    16141 taacagatgt aattaagttt actatatggt ttcttgaatt gtttttttag tacttattta
    16201 tatctataca taaatgtatt tggtataagt aaaataagag taatttacta taaaaaggaa
    16261 tttaatgatt gagtgagaat ttattactta aatattaata attaaataga tatattttta
    16321 actatatttg agatatatca ttgtgaaaaa ataaaattta taagtattat tgtgaggtta
    16381 taagtagatt tatttaaaaa taaatttatt tatagccttt ttaaatttga aataatattc
    16441 ataaggtaat aaaagttaca aatattttct tcgctaataa gttttaaaat acctataatg
    16501 ttttactttt tattataata aatacaataa atatattctt atataaattc tatgtcagat
    16561 atatggaaag ataagaacct tgagattatg ttaaaaaatc tagattttat acttataaaa
    16621 agtttgattt tacaacctta caaaagagaa tcaagatagg aatataatta tctttttcat
    16681 aaatatataa atgatatact aatattatag tattattgat atttgtattg gaggttgaac
    16741 atgagggatg aaattttaag ttttgtaaat gaatgtgtat tatttaacaa ctttgatgaa
    16801 cttaaaaaag aaagtggcat aataacagaa gatatagtaa aaaagtttaa tttaagtaga
    16861 actcaatcaa gtagattatt aaatgattta gtaaaatctg ataatcttat aaagataaac
    16921 agtagacctg ttattttttt acctaaaaat aaaataaatg atgtagtagg acctacaaaa
    16981 aaaagtattt atgaaaattt agatgaactt aaaaatgagt gtaaattatt aaagcaagag
    17041 actgtgttta atagtatcat aggatatgat aatagcttaa aagaagtaat cgaacaaata
    17101 aaaaccgctg tactttatcc aaatggcgga cttacaataa tgctgacagg tgaaagtgga
    17161 gtaggaaaaa cttttttagc caagataata tacaaatata gtgtaagcag taatgtatta
    17221 gaaaaaaatt ctccatacaa tgtgttaaac tgtgcgcagt attataataa tttggagtta
    17281 ttgtctagtc tattatttgg gcatgtaaaa ggagcatata ctggagcaaa tgaatctaga
    17341 gcaggtctaa tagaacaatc agatggtggt gttttatttt tagatgaagt acatagatta
    17401 aatagtgaag gtcaggaaaa gttatttaca tttatggata caggtacgtt ttctaggatt
    17461 ggagagaatg gagtaattag aaaagctagg gtaagattaa tatttgcaac aacagaaagt
    17521 ctatccaaat ttttgcaaac ttttttaaga agaataccca taaatattta tgttcccaat
    17581 atagaagaaa gagggactat tgaaaaaaga aagattgtag aacattttat ttatgaagaa
    17641 agtaaggtac ttggcttgcc tatagagata actcaaatta cattaaatac gattttaaag
    17701 ataaaattca aggggaatat aggtgaatgt aaaaatgtaa taaaatatgc atgtggacga
    17761 gcttatgcaa aaaatcaaag aagaaatgga aatatttttg ttactcttca agacctccct
    17821 agaaatcttt atatagaaaa tgtagatata tttcaattcc aaagtggtaa aagcaaaagc
    17881 attatctttt ctcctaaaca atttagtatt gaaatgtcaa atggaagaaa taaaagaaat
    17941 ataatctatc aaagtttcat aaaatgttta gactactttg atagactaga aaaaaatgaa
    18001 ataacaaaag aaatatttag taaaaagagt tctaatctaa tgattagact aatggacaat
    18061 ttagtctttt caaataatga tgaaaataat aacgtactta tggagcttat aatatcgata
    18121 atgcaggata tatttagaaa tttagaagtt aataacaata taaaatacga tgggaataat
    18181 gtattggcat tttcgtctta tctttatttg aaggacgaat cttatcaatt atctgatgag
    18241 tataaagctt tagaaagtaa attactaata tatttaaaaa aagagagtta caaagaatac
    18301 tctataataa gtaaaatatc aacattaata acgaatagat tggatatgta tcttaataat
    18361 gtagatacaa tcataatgat tatatttatt aaatcaacaa ttataagaat gaaatttaat
    18421 caagttaatg cgactattat agcacatgga tatgctactg ccagtagtat ggcgaatgta
    18481 tgtaatcgga tgttgggtaa aaatatattt acagctattg atatgcctat agatgctacc
    18541 attgctgatg tatcaaaaca aatgtctaga tacctagaag gagataggat aaagaatggt
    18601 ttaattgtgt tatttgatat gggctctctt aatcttatct atgaagattt aaaaaataag
    18661 ataaatgggc caatgttatt tatagaccaa atatccacta tttctgcact agaagtagga
    18721 aatttattaa tgcaaggtaa aaacattgaa cagattgcag aaattatgga tgaagaaact
    18781 aaacctaata tacaattgta ttatcctcaa aaaaataaag aatatgctat aatatcatct
    18841 tgttttacag ggataggtac tgctataaaa atccaacatc tactttccga aagtttaatt
    18901 gggatagttg atgtcaaaat aataccttat gattatgata gtcttgtttc tatgggagat
    18961 aaagaaccag tttttgaaat gtatgatgtg atggctatag taggaactgc taatccaaat
    19021 gtgaatgggg tggattttat attgttagag gatataatat caggaaaagg agaagatgat
    19081 gtttttagaa tatttagtag agtagtagaa aatgaaaaaa taaagaacat aaatgattct
    19141 attgtaaaga atttttcatt aattagagta atagacagtc taactatttt agattcaaaa
    19201 aagataatag agagaataga agaaggaatc aatgctatag aaaatattca aaagaaaaaa
    19261 cttccaaatg ataaaaagat ttctttatat gttcatttaa gttgtatgat tgaaagatta
    19321 gttagacaaa ctgaaattga agagtatact gatatggatt tgttaatcaa aaaccatcat
    19381 agtgaagtaa aactaataaa aaatgcattt agtgtgctag aaaagagtta tagtgtgcaa
    19441 ataccaattt cagagatagg atatatttat aatattatct atggtctacc acagtaaaat
    19501 acaagattac aaaataagta aatgaaattt aaaatagtat gccagtattt ggcatacttt
    19561 ttgcttttat agataaatga gagtataaac aataaaaatt ttaatgtatg atggaggatt
    19621 tggggaaaat ggtatctttg atgagaattg atgacagatt gatacatggt caggttgcat
    19681 atggatgggc aacatcacta ggtataaatg taatactagt tgcaaatgat gaagcaaaaa
    19741 atgaccaaat gaaagctatg tcactgaatt tagcgaaacc tagcaatgta actttatata
    19801 taaggggggt agaggaatct ggagaaatag tagaaaaatt tgcttcttca aagaaaagca
    19861 atgtattagt gttagtaaaa aataccagag atgctttagt tgtagctaaa aattcaggag
    19921 gagtattaaa agaaataaat gtaggagggt taagatatga agaaggaaaa aagaaactta
    19981 cagatttagt tgctgttgat gatgatgata taaataactt taaagaaata ggtgatttag
    20041 gaattgatat agaacttaga atgttaccta gagacaaaaa gaaacaacta tcagatttaa
    20101 taaaataatt taggagaaag agaatgaaaa aatttctaat tgcaacacat ggagaattgt
    20161 ctagagaact tgttgacaca agtaaattaa taattggttc attagctaat attgagtatt
    20221 tttgtatgac aaaagataaa tcagcagatg atgctgagaa agaaattaga agtatattat
    20281 caaataataa agatagtgaa gaatttgttg ttttaacaga tgtgtttggt ggaagtgtgg
    20341 caaatatatg tacaaactta cttttgcaag ggttcaaatt tgaattgttg acaggcgtaa
    20401 atttaccaat gcttttatct atattcttat tagttgagga ggatacaaaa ctaatagtta
    20461 aatcaggctt agaggaagca aaaaatggaa ttgtttatgt aaatgagtta ttagaaaatc
    20521 gaaataaaat caaaaatgta atgtaattta atgtaaatta aactaagtta aaatggaggt
    20581 tcaatatgtt agtacaagct actatgttag ctattatagc aggagttggt atactagatg
    20641 ggcgtatatt tggtcaatct atgtttgata ggccaatagt tacaggtatg ttagttggat
    20701 tagttttagg agacataaaa tcaggaatta tgattggtgc acagcttgaa ttaatttgga
    20761 tgggaattgc agggataggt gcagctactc ctccagacgt agtaacagga ggtgtattgg
    20821 gtacagcttt tgctatatta tcaggtaatg gtgcagaagt tgctttagca gtggcagtgc
    20881 cagtagcagt attagcacaa tctcttggag tattagttag aataataaat tcgtactttt
    20941 ctcaaaaggc agttttctat gctaaacagg ctgattttaa gaaagttacc ataatgatgt
    21001 ggttgccagt aatattattc tttttaagta catcagttcc aaccttttta gcaattatga
    21061 ttggtgctga taaagttaca acattgataa atgcaatacc taaagttata ttagacggat
    21121 tgggtgttgc aggaacattg ttgccagcag taggttttgc gttgcttatg gatatgttga
    21181 tttcaaaaaa gatgtctgta tttttcttta tagggttttt gttagcatct tatggtggat
    21241 tagatataac tgctatagct ttatttggag tatgtgtagc atttgtacta aacttttata
    21301 tcaataacaa agaaaataat caaaatcctc aattagcaat gaatacttta acggaaggag
    21361 agattgactt tgaataaatt aacaaaaaaa gaattaagaa gtatgttttg gcgttcattt
    21421 gctcttcaag gtgcatttaa ttacgaaaga atgcaaaact taggttattg ttattcaatg
    21481 ttacctgcaa taaagaagct atataataag aaagaaaatc aagcaaaagc aatagaaagg
    21541 catttagaga tttttaatac aacgacagtg gtagttcctg ctatattagg tataacagca
    21601 gctatggaag aagaaaatgc taataatcca gaatttgatg aaagttcaat tagtgctgtt
    21661 aaaacagctc ttatgggacc tcttgcagga attggtgatt cattattctg gggaacattt
    21721 aggattatag cagcaggagt aggagtatca cttgcaaaag aaggcaatat ctttggacca
    21781 ctattattct tattattata caatatacca gcatttgccc ttagaatatt tggacttaag
    21841 tatggttata aagtaggggt aaattctctt gaaagaatac aaagagaggg acttatggag
    21901 aagataatgt ctatggctac aactgtcggt ttattcgttg taggaggaat ggttgcaact
    21961 atgttgagta taactactcc tttgaaattt aacttaaatg gtgcagaagt aattttacaa
    22021 gatatactag ataaaataat acctaatatg ttacctttaa cctttgcatt tttaatatat
    22081 tacatgttga agagaaaggt aagtgtaaca aaattaacta taggcactat agtaacagga
    22141 atcgctttac atgctatagg tttgttatag aaattaatta taatttattg aatatataga
    22201 taaggaaggt gtataaaatg acaatacaag attatatgtt agaaacacca gttagaatga
    22261 gagaaattat atctaatgca gatagcttat ttaatgaagt taagagagca aatctaaaaa
    22321 agattataat aacaggttca ggtactagct atcactcagg agtgcaagtg caaccatatt
    22381 tacaaaattt attagatata gatgttgtga aaatgtatcc atttatgata actgaagata
    22441 cattcaagtt tgataatgaa aataccctag tagtaggtgt atctcaaggt gggagtagtt
    22501 attctacata caacgctatg aagttagctg aagataaggg ttgtaaaata gcatctatgg
    22561 caggttgtaa gaatgcttta atagatgaga tttctgatta tatattaaca gtaaattgtg
    22621 gtgaagaaaa atctggagct aaaacaaaag gatattattg tacaaagtta aacttaatgt
    22681 tattaggtct tcaaatagct agagaaaaag gtacaatatc tagtgaaaag tacaatgagg
    22741 aaataaataa gatattagat gcaattaata gatttgaagc agtgtataag ctctctaaac
    22801 aatggattga aagaaacaaa gaaaagttag ttaattctaa agaaataaga ataataggtc
    22861 actctgatat atatggagat acattagaag cagcattaaa attattggaa acaatgagaa
    22921 tacctgtaac tgggtatgag tttgaagagt ttatacacgg tatatataat gctataaata
    22981 gtgattctac tatatttata ctagatacag gaaaagaacc tagagtaact aagatgattg
    23041 atgtactttc tggttggacg gaaaatgtat ttgctatagg tagagatgtt acagaaaatg
    23101 ataaaaattt aaagattgat atcacagata atccttacta tcaaacattt aattttatag
    23161 tacctataca actaatatgt ggggaaatac ctacattaag aggcgtagac ccaagtgttc
    23221 caaaagatac taggttccac atgaagttag gtagcaagaa actgaataag taacaaaaaa
    23281 agaggttgtc tcaaaataga gataagcctc tttttgactg ttgaaatgat gaaaatatat
    23341 ttgattttat ataaaaaaga gtgcctcatg aactaaaaag ttaattttga gacacccact
    23401 tttatcttat tttaaactaa aatatttata tattagtgtg tattactttt tttattgctg
    23461 tcttccttac ttgagcaaga attagcactt ggacatccag agcaaataga acatccacta
    23521 cagacatttc cgttttttgt agacttgaat ttattaaaca gaataaagag tgcaaggact
    23581 ataattattc cacctatcaa agcttccatt attgaattac ccataaatag tactccacct
    23641 atgcttttaa ctataaaagc cattatccat gctagtataa attgatatgc aaatgatgta
    23701 aacatcattt tattgccata ttcttttttc atagttgcaa gtgcagctat acatggagta
    23761 tataaagcag taaatatcaa gaatgtatat gcagtaactc cattaaatat tgttggtaag
    23821 acaacagtta gattaccata tagtatattt aaagagttta ctacgatttc ttttgctcca
    23881 agaccactta aaatagcaac tgaagttctc caatcatcaa aaccaagagg tttgaatata
    23941 ggagatataa tatgacctag agtagccaag aagcttacat ttatatcttc tgtatatcca
    24001 aaaacattga atgaagaaag tccccaaatt acgactgaca ttgcaaacat gacagtaaca
    24061 actcttatca agaaaccttt tgatttattc caagtatttt taataagtgc atctatagta
    24121 gggattctat attctggtaa ttctaatata aatggttcag cctctgtttt aaaggctgtt
    24181 ttatttaaga ataatgcaac tacaattgcc ataactatac ccaccaaata cagtgacgta
    24241 gttactaaag cagcattatg tggaaagaat atagctacaa atagtgcata tataggtagc
    24301 tttgctccac aagtcataag tggtgcaata agagctgtaa ctcttctatc tttttcgctt
    24361 tccaatgttc tagttgccat tatagctgga gaagaacatc ccatacccat aactataggt
    24421 ataaaagctt taccagatag accaaccttt ctcataactt tatccattaa aaatgctgtt
    24481 cttgacatgt atccactatc ttcaaacaac gatataccaa agaataatgt aaatattaaa
    24541 gggaagaatg gtaaagtacc accaacacct ccaattattc catcaactat aagtgattta
    24601 aaccatgggc tagagttaga taacatatca ctaacaggtg cagaaatata agcctctata
    24661 agactagcaa atccctcttg tagtggtcct ccgacccagt caaaggtaaa tttaaataag
    24721 aggaacaata caccaataaa tatcggatat gctaatatag gattcaagac tatattgtct
    24781 atcttatcac tgatagattt cttttgtgta tatgtagttt ttgtacatct cgataaaatg
    24841 gattctaatt ttttatatgt ttctgtttca cttccaaagt ttttctgata tataaaagct
    24901 ttttgcgtag ctcccttaat ctcttcttct atcctatcaa caccattttt cttttttgct
    24961 ataattggaa caacaattac tccaagttct tcacttaatt ttttggcatc tatattaact
    25021 cctttagatt ctgcaatgtc taacatatta agcaatataa ctataggctt gttaaattgc
    25081 ataagttgag ttgttaagta aaggtttctt gataaattag aagcatcaac tacatttact
    25141 attacatcaa catcttcatt ttctaaatat gatttagaaa ccttttcttc atttgagaaa
    25201 gtatccattg catatatacc aggtaaatcg acaaccttta tctcttttcc aagaaatcct
    25261 tctttttttt ctatagtaac accaggccag ttacctacat attgatttga acctgtaagt
    25321 agattaaaaa cagtagtttt accgacattt gggtttccta ataatgctac attaatcacg
    25381 ggtagctcct cctctattat tgtaaagaaa tatttttagc atcagatttt ctaatcgcaa
    25441 ggtcaaagcc tctaaatcta attataattg ggtctccaag tggggcaacc tttttaactt
    25501 ctatttcagt actatttata catccaagtg caagcaatct tttagataat ttaatatctc
    25561 catctatact attaataaat cctttttctc ctatttttaa atcgtaaaca gtcattatta
    25621 acacctccta atgaaattca ttatcattaa aattgtaaca agtatgatac aaaaagtcaa
    25681 gatttataga ggtaattgca cattgaaata actaatattt attctaaata gattaaaaat
    25741 attttagtct aaattaggtg tttttatata ttttgtatta taatgtaaga tgatagataa
    25801 aatttggagg agttaactta atgaaaacta gaaaaatgac atttttaggt cttatgatag
    25861 ggtatagttt agctttatat gtactagaga catatatacc aaatcctctt atagttatat
    25921 ttccaggggc aaaattgggt ttgagcaata ttataacttt aatttcatta atgttgttag
    25981 gtgtcaaaga tactgttata attgttacta tcagggtaat tttgtcatca atatttgcag
    26041 gccctttatc atatctacta tttagtatag gaggagctta tctgagtctt ttatttatgt
    26101 atttagcgag taaaataaaa gatttatcac taatagggat aagcattata ggtgcaatag
    26161 ggcataatat aggtcaatta atagttgcaa gtttaatcat agaaaatata cttgctatag
    26221 ggtacttacc atttatgtta atggcatcat tagtaactgg tatgtttgta ggcttagtat
    26281 ccaggtatac aatacctaaa ttaaagcatt tttatagtaa gttttattga cataatattg
    26341 acaaaatata atattgtttg ataaaatgta tataaaaata tacaaatttg taatagtggt
    26401 gattattatg gcgaacaatg aattagaagc gttaaaaaat gagattgaag cagttaggga
    26461 agagataaat acatacatag aatatccaga aatatttcaa gaagagttag tagaatcaag
    26521 taagaaaata gatatattaa taaataaata catgtaccta agcaaatagc ccacagttgt
    26581 gggttttttt gtatcaaaaa ttaacagatg gctatatttt acatatatta ataataatat
    26641 taagatataa aatagtattt ctgatgtgta aattatctgt aggacataag ttgtaattaa
    26701 gtcaggaggg ttttatatta tgattgatat aggaagtaag gagatagcaa taaggttact
    26761 attagcacta ataataggag gaataatagg cttagagaga gaaaaaataa gacagtttgc
    26821 aggatttagg acacatatac taattgcaat aggttcttgt attgtttcaa taacttcgat
    26881 tcaattattc tttgattata atttgtatac aaatgctgac ccagcaagaa tgcctgctca
    26941 agtattatct ggaattggtt ttttaggtgc aggagcaatt ttaaaaaata aaggtggagt
    27001 aaaagggctt actacagctg ctgggatgtg ggcaactgca tgtattggta tagcagttgg
    27061 atatggttat tatgaattgg caattctagg tggattattt gtaatgatag cattatttgt
    27121 gcttaggata atcaatacgt ttttatttag ccaagaaaag aatgtattga tattaaaatt
    27181 aagcaatttg gactctatac cttttttata tgataaattt gaaaaaaatc aacttatcgt
    27241 aagaaatatg gaaatagaat gtcgtaatga agaatactgg aaggttaatt tttttgtatc
    27301 ttatgctaag agaatacact tacacgacgt tatgaaagaa ataaatatga tagaaggagt
    27361 aataaatgta gattatttgg actaaaatat atacaatata ttttgtaaag tgaataaaat
    27421 ataaaacaaa ttaaaataga ggtgtatgat atgggaactg atagaagtac aatagaagaa
    27481 aagtacaaat ggaagataga taagatgtac tccagtaaag aggaaattga aaaagacata
    27541 agtaaagtaa aaaatcttat ccaagaagta aaagaatata aagggaagct ttctgagagt
    27601 aaggagaact tatataaagt attaaatatc tctgaaaatg catctaggat tgttcaaaat
    27661 ttatatgtat atactcatat gaaacaacat gaagatacta gaattaatga taatcaagga
    27721 agagccacta agacagaaat gttatctact gatttaggtg tggctacttc ttatatagtt
    27781 ccagaaatac tggcgataga agagaataag ttaagtgaat acttaaaaga tgagaagcta
    27841 tccttttata caaaatatat aaaggatata ttgagagata aaccacatac tttgagtgaa
    27901 cgagaggaag aaatacttgc agctacatca gaattatcga caattccaga aaatgtatac
    27961 gatatgttag catatgcaga tatggaattt cctgagatag aagatgagga gggtaataag
    28021 gttaagcttt ctcattcaaa ctactcttta tttataaaaa gtaagaatag tagagttaga
    28081 aaagacgctt ttgaaggtga attttcaaca tatgagaaat ataaaaatac atttgcctca
    28141 acgctttatg gtggaataaa atcagagata ttttacgcaa aaactagaaa gtataattca
    28201 gcaatagaag cgtctttatt ttcagatgat gtaagtttag atgtttacaa taatcttata
    28261 tcagctatag ataaaagtat tcctaatcta aataaatatg ttgagcttaa aaagaaattt
    28321 ttaggattga atgaaataca catgtatgat ttgtatgttc ctttgacaga taaatttgac
    28381 atggatatac catatgataa agcacaggat atcatattag aggcacttaa acctttagga
    28441 gaaaaatact tgaaggtcat aaaaagtgct tttgatgaag ggtggattga tgtatatgat
    28501 aatgaaggta aaaaaggagg agcttattct tggggaagtt atgattcaca tccttatata
    28561 ttaatgagct ataaaaatga ccttaattct ctattcactt taattcatga attagggcac
    28621 tctgttcaca gtcattattc aagaacaagt caaccatttt tatattcgga ctataagata
    28681 ttcgttgcag aagttgcatc tacattaaat gaattacttc ttataaatta tttattagaa
    28741 aattctaagt ctaaggatga aactatatac ttattaaatt attatttaga acaatttaga
    28801 actacagttc atagacaaac tatgtttgct gaatttgaaa aaattgtaca tcaaagggta
    28861 gagagtggag aaccacttac tgctgatgaa tttactaata tatactacaa attaaatgaa
    28921 aagtattacg gaaaatctgc aatagtagat aaacaaatag gaatagaatg ggcgagaata
    28981 cctcattttt attcaaactt ctatgtatat aagtatgcca cagggttttc tgctgcaagt
    29041 gctttaagtc aacagatact ttcagaagga agtactgcag tagatagata tataaacttc
    29101 ttaaaaagtg gagggtcaga atacccacta gaacaattaa aatctgctgg tgtagatatg
    29161 actaaaaaag aatctataga ggaagcctta aatgtatttg ctgaattagt taataaacta
    29221 gaaaaagaat tataggatgt gataggttga tagcttcaat gaagacttct gataatatga
    29281 gttgtattgt gtttatatct gcctaaagta actttaaaaa gttaatctaa ttaagaataa
    29341 atcatataaa attaagtttt tacatgtatt taacaatatt tacatttact taaccctgct
    29401 ctaatattca tttaacaaag aatctgtatt attaaaatat agaaaaatga caacaaaaaa
    29461 ttaaaatata agcgggggta agtaaaatgt ttaaaaagaa aataggcgtt cttttagcta
    29521 gtacaatgtt agttggagct ttagcagtag gatgtagttc taactcagga ggttcaggtg
    29581 attcaggaga ttcaagcaaa gtttctgttt caggttcaac ttcaataggt cctcttatgg
    29641 aaactatcgg agaaaagttt caagaaaaaa atcaaggtgt tagtgtagaa gtacaacaga
    29701 ttggttcttc cggaggtata aaaaatgcca tagaaggaac atctgaaata ggaatgagtt
    29761 caagagattt aaaagatgaa gaaaaaagtg ctggattaaa ggaaactaaa atagcaatag
    29821 atggtatagc attgataact aatactgcaa actcagtaaa aaacctgaca atggaccaag
    29881 ttaaaggtat atatacagga aagattacta actggaaaga agtaggagga aatgatgcac
    29941 caatagtaat agtatcaaga gaagaaggtt caggaactag agatggattc caagaaattg
    30001 tagggtttga ttcagaagaa ctaactaaag atgcacaaat aagtgatggt tcaggaaata
    30061 taaaaactac agttgaagga aatgaaaatg ctataggata tatatcattt ggatatgttg
    30121 atgagaaagt aaatagttta aaaataaatg atgttgaagc aactgatgca aatgtaatgg
    30181 ctaataaata tccaatatca agaccattta tattagttaa taaagaagat ggtttgtcag
    30241 aaaaagggaa agctttaata gattatatat taagtgatga aggacaagca cttgtttcag
    30301 aaaaaggatt tattaaagtt aatcaataac atgaattata atataaagta aacatataat
    30361 accctgtgag ataagctcat aaaaatgatg ctttaacttg cagggtattg tctattattg
    30421 aatgtagaaa tataaatatc tttagataaa ataggtattt tagtatctta tatctaaaaa
    30481 tatggaaata ttgacaaaat aatattacaa tgttagaata aaaaagaaat ttatgaaaat
    30541 atataaaaga tttttaataa aaatatttta gaaactgaga agtatatctt attattgggc
    30601 atctggagat atatggagtt agtggtgcaa ccggctatga atataatttt tatattgcat
    30661 agtctttttt tgctttaaaa ttaacaaaaa tgttgcaaaa tttgaaaata agagtattag
    30721 tcgttaagat ttttattgat aggtgaaatt ttggctttt
//
LOCUS       DAEPOG010000025        30384 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEPOG010000025 DAEPOG010000000
VERSION     DAEPOG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.025"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            86..1144
                     /locus_tag="KR432_001018"
     CDS             86..1144
                     /locus_tag="KR432_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBH0639569.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KR432_001019"
     CDS             1398..3422
                     /locus_tag="KR432_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0639570.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KR432_001020"
     CDS             3642..4328
                     /locus_tag="KR432_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBH0639571.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KR432_001021"
     CDS             4423..5478
                     /locus_tag="KR432_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBH0639572.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="KR432_001022"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="KR432_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0639573.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="KR432_001023"
     CDS             7194..8900
                     /locus_tag="KR432_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0639574.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KR432_001024"
     CDS             8922..9995
                     /locus_tag="KR432_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBH0639575.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KR432_001025"
     CDS             10007..10729
                     /locus_tag="KR432_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0639576.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KR432_001026"
     CDS             10745..11902
                     /locus_tag="KR432_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH0639577.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KR432_001027"
     CDS             11905..12744
                     /locus_tag="KR432_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0639578.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KR432_001028"
     CDS             12759..13934
                     /locus_tag="KR432_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBH0639579.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KR432_001029"
     CDS             13963..14712
                     /locus_tag="KR432_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0639580.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KR432_001030"
     CDS             14734..15642
                     /locus_tag="KR432_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0639581.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KR432_001031"
     CDS             15690..16442
                     /locus_tag="KR432_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0639582.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="KR432_001032"
     CDS             16420..17763
                     /locus_tag="KR432_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBH0639583.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="KR432_001033"
     CDS             17769..18860
                     /locus_tag="KR432_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0639584.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KR432_001034"
     CDS             18944..20857
                     /locus_tag="KR432_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0639585.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KR432_001035"
     CDS             21056..21763
                     /locus_tag="KR432_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0639586.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KR432_001036"
     CDS             22065..24176
                     /locus_tag="KR432_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBH0639587.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KR432_001037"
     CDS             complement(24374..25339)
                     /locus_tag="KR432_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0639588.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KR432_001038"
     CDS             complement(25624..26580)
                     /locus_tag="KR432_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0639589.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KR432_001039"
     CDS             26948..27736
                     /locus_tag="KR432_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0639590.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="KR432_001040"
     CDS             complement(27859..28329)
                     /locus_tag="KR432_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBH0639591.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="KR432_001041"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="KR432_001041"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBH0639592.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="KR432_001042"
     CDS             29155..30060
                     /locus_tag="KR432_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0639593.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12045 a         3305 c         5391 g         9643 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata attagtatat ataaagttta actgtaagat aatttaaaaa
    30361 ataggggaat ttatatgaat aaaa
//
LOCUS       DAEPOG010000026        30345 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEPOG010000026 DAEPOG010000000
VERSION     DAEPOG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.026"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..408
                     /locus_tag="KR432_001043"
     CDS             <1..408
                     /locus_tag="KR432_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0639594.1"
                     /translation="FGGKIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFFYD
                     LVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVTCK
                     LYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            873..2087
                     /locus_tag="KR432_001044"
     CDS             873..2087
                     /locus_tag="KR432_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639595.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2507..3028)
                     /locus_tag="KR432_001045"
     CDS             complement(2507..3028)
                     /locus_tag="KR432_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0639596.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3384..4181
                     /locus_tag="KR432_001046"
     CDS             3384..4181
                     /locus_tag="KR432_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0639597.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4291..4881
                     /locus_tag="KR432_001047"
     CDS             4291..4881
                     /locus_tag="KR432_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH0639598.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4947..6644
                     /locus_tag="KR432_001048"
     CDS             4947..6644
                     /locus_tag="KR432_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0639599.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6858..7697
                     /locus_tag="KR432_001049"
     CDS             6858..7697
                     /locus_tag="KR432_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH0639600.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            8214..10070
                     /locus_tag="KR432_001050"
     CDS             8214..10070
                     /locus_tag="KR432_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0639601.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    10153..10393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    10459..10694
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10779..11996
                     /locus_tag="KR432_001051"
     CDS             10779..11996
                     /locus_tag="KR432_001051"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0639602.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            12110..12604
                     /gene="cdeM"
                     /locus_tag="KR432_001052"
     CDS             12110..12604
                     /gene="cdeM"
                     /locus_tag="KR432_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH0639603.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            12869..14134
                     /gene="hisD"
                     /locus_tag="KR432_001053"
     CDS             12869..14134
                     /gene="hisD"
                     /locus_tag="KR432_001053"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH0639604.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(14337..15089)
                     /locus_tag="KR432_001054"
     CDS             complement(14337..15089)
                     /locus_tag="KR432_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0639605.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(15207..15476)
                     /locus_tag="KR432_001055"
     CDS             complement(15207..15476)
                     /locus_tag="KR432_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639606.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            15951..17045
                     /locus_tag="KR432_001056"
     CDS             15951..17045
                     /locus_tag="KR432_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0639607.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            17047..17997
                     /locus_tag="KR432_001057"
     CDS             17047..17997
                     /locus_tag="KR432_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH0639608.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            17990..19174
                     /locus_tag="KR432_001058"
     CDS             17990..19174
                     /locus_tag="KR432_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639609.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            19187..20683
                     /locus_tag="KR432_001059"
     CDS             19187..20683
                     /locus_tag="KR432_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0639610.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            20706..21656
                     /locus_tag="KR432_001060"
     CDS             20706..21656
                     /locus_tag="KR432_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639611.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            21677..22639
                     /locus_tag="KR432_001061"
     CDS             21677..22639
                     /locus_tag="KR432_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0639612.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            23011..23643
                     /locus_tag="KR432_001062"
     CDS             23011..23643
                     /locus_tag="KR432_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0639613.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            24054..24146
                     /gene="kdpF"
                     /locus_tag="KR432_001063"
     CDS             24054..24146
                     /gene="kdpF"
                     /locus_tag="KR432_001063"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH0639614.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            24187..25869
                     /gene="kdpA"
                     /locus_tag="KR432_001064"
     CDS             24187..25869
                     /gene="kdpA"
                     /locus_tag="KR432_001064"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH0639615.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            25894..27951
                     /gene="kdpB"
                     /locus_tag="KR432_001065"
     CDS             25894..27951
                     /gene="kdpB"
                     /locus_tag="KR432_001065"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH0639616.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            27970..28596
                     /locus_tag="KR432_001066"
     CDS             27970..28596
                     /locus_tag="KR432_001066"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH0639617.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            28833..29741
                     /gene="cysK"
                     /locus_tag="KR432_001067"
     CDS             28833..29741
                     /gene="cysK"
                     /locus_tag="KR432_001067"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH0639618.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            29768..>30345
                     /gene="cysE"
                     /locus_tag="KR432_001068"
     CDS             29768..>30345
                     /gene="cysE"
                     /locus_tag="KR432_001068"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH0639619.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNV"
BASE COUNT        11967 a         3054 c         5515 g         9809 t
ORIGIN      
        1 tttggtggca aaataataag aagaaatgaa aagactaagt ttgactggtt aagtagagat
       61 aaagaacaag ttgaaaaata tataaatgac ccattttgtg gagtagtgtg tagttgtggc
      121 tttttttatg atttagttca agggttaaaa gaaattgagg ataaagaaaa cttaaaaaaa
      181 gtgccacttg atatacctat ttacataata tctggagata aagaccccat aggaaaaaat
      241 ggtaaaggag tcttaagact aagggataga tatataaaat taggagtaaa agatgtaact
      301 tgcaaacttt acaaagatgg aaggcatgaa ttgttgaatg aaataaatag agaagaagtt
      361 tttgaggata taatttgttg gttaaacaat aaaatagaaa tattgtaata aaaaaatata
      421 atgatatatt tgctttaaat ttatttatat ataatatttg aaaagtggat tatgtatatg
      481 caaagtagaa aagagagagc ctagaagtga ttttttgcta taatgccatt tattgaatag
      541 gtgggtgtta ttataaataa attattaaca ctcagtatta ttatcattgt atatggaatt
      601 tattttattt aaaattcctt gaaagtttaa atatagtaag ttgtagtaaa gagaaaatct
      661 aaatatttaa atgctattta atgtgaatct attaattagg ttatacttaa acttaattaa
      721 tagattttat atttcaagcc aatgagatag ggttttagat taaaatataa ccaatggtta
      781 aattacagac attttgttaa ctttccctcg atttactagt aaatattttt tttatatact
      841 taaattacaa cagatgtact agggaggtat atatgcaaat taaaataggt aaagtcatac
      901 agcgtcttag aaaagaacga aatttaactc aagaacaatt agcaaagttt ataggtgtgt
      961 caacaccagc agtatctaag tgggagagtg gaaattctta cccagatata gaattattgc
     1021 ctttattagc agactttttt aatgtgtcta ttgataagct tttaaattac aagattgatt
     1081 taagtgaaga aaaagtaatg aaaatttata aagaattaga atcaggcttt gctagaattg
     1141 aaattgattt atcaacagaa gaaccaaaag aagaatttag acaagactta gaatctgtaa
     1201 aaaagctttc taatatgtat atagagaagt atcctaaaag ctatttattg aagctaagga
     1261 tatgttcgct gtatcagatg tattcatata aatttggtaa gagtgaactt aatgacagag
     1321 taaaagaaac aacgaatatt cttgaagata tagtgagaaa tacagatgat atccaaataa
     1381 aagaaacagc tcttattata ttatcaaacg catattgtat gttggaagat tatgaaaagg
     1441 cagagttgta tttaaatatg atacataagt caatagggga tacaagtgtt aatttagcta
     1501 tgatatattt aaagcaaaat agacttgaag aagcagaaat attgcttcaa aataaattgt
     1561 ttagtaatat atttaatata agcatggatt gtaagggaat aattaatgta tacaaaaatc
     1621 aatacaagga attaaagaaa aaattggaga atagaaattt taataaaaat gcaattgaga
     1681 gagaaatgga gtatataaaa aataagttgc tgtggtatgc aaatctttct ttagaaataa
     1741 aaaaaatgct cagtgaagat aaaggtgcat tttttagtat gtatgtggat tatatggaat
     1801 tatctctaat ttttttattt tttaacatga aagaagaagc aaaaaaagcc ttatatagtc
     1861 taaaagaaat attagaaaaa tatcctatac atgaaagttt ggatgtaagt caaatgagat
     1921 tttttgataa ggtagagtct aaaaatttat atacttttaa tatatataca aacttgttaa
     1981 ttgtacttaa tgatgacagt tataatgagc ttagagaaga accaattttt aaggatgtaa
     2041 ttgaaaagat tttagatatg gaaaaaatgt tgaaaaataa agaataagta aaaatttaat
     2101 ataggactta aagattaatc aaattaaaag aaagtgtttc aaaataaaaa tttgaatcac
     2161 cttttttgtt ggtcttaaac cgttatttag aaaaatagag gtttcaaaat agaaatctga
     2221 attatctgta ttattacaca aaaatagaaa cctaaattta taaaaataga ataaagtttg
     2281 ttatataaaa tgtaattttg tctataaaat ctcacatgtt ttcttaactt taagcgtgaa
     2341 aaagaggatg tatcatgaat tttttagttc atgatgcatc cttatttgta tgaaatccaa
     2401 tatatcttta ttatttaaac aatggaaaag agtcttattt ctattttgag acatctcttt
     2461 tatttaatta tgaagtttta tacagttcaa taaataggaa ttatatttag aaactcatga
     2521 cattatttct tagatagtga ttaaatttat catattccat tttagataac ttttctgggt
     2581 aagacattac aatcaggtta tgcccattga atttttcatg gtatggtgga tgaatgtgtt
     2641 cataattatt tgtcttatat tttactcttt catagaacgc cttgcgataa attgaaatct
     2701 catcaattgg aaggtcaatt tctaaaataa tagtttttcc tttcttattt aaaagctcta
     2761 acgctctttg tccatatcta ttattgcgca tttctggaaa aatgcagaaa tgttctacat
     2821 agataaagtt atctgtttcc caacataaaa ttatgccgac aaactggttt ttatcataaa
     2881 ttaaattgaa ttgatattct ttatttttca aaatttctgt ttgcaaataa gattttcttt
     2941 gttcatgaaa tgggaaacta attttatata attccatagc cttgtaatat aatttatttt
     3001 cactggttct taaccgttca aatttcactt tactcctcct aatcacgaaa attccaattt
     3061 atttatgagt tcattttacc atagtcagta tttgcataca atgtgtttta agttgagtgt
     3121 catttctttc ttttatttat gcttatatct catagactaa gagataattg cttaagacat
     3181 aactaaaaac tattatatta agctttgata atctaaaaac agtatattta tggacttttc
     3241 taggtctaaa agtaatctta ataattttag aagttacaaa aaagttacat aaatttggaa
     3301 agctataata tacatatata atataaggtt aaaactgtaa tgaataatat taatttaaaa
     3361 tttgaacatg gaaggagatt attatggaga aaagagaaaa gaggataata atatcatctt
     3421 tattaagtgt atcaatttta atgggtttgg taagtatata ttctatatta aataaagaag
     3481 acataatttt aactgttaag ggtcaagagc aaaaagtatc ttcctttaag aaaacagttg
     3541 aggaactttt ggatgaacag ggtgtaaagt ataattctga agataagatt aatccaagtt
     3601 tagatacaga actaaaagat gatatgaaaa taaaagttgt taaggtaact aaaagtaaaa
     3661 aagaagagat tgaaaaaatt ccatttgata caaagcatgt aaatgatagt aatttgttaa
     3721 aaggaaaatc taaagtttat caagaaggtc aagaaggaga aaaaaaacta gtctataatt
     3781 taacttacca tgatggaaaa ttagtcaaaa aagtcttatc aaaagaagta atatctaagg
     3841 agccaactac aaaaattata aaatatggaa ctaaagaaaa agtactaata gcatcaagag
     3901 gagcaaatat aagaggaggc aaacatatga aagtggttgc cactgcatat gcaggagata
     3961 caataacatc tactggtaca actccaagat ggggtgttat tgcagttgac ccacgtgtaa
     4021 taccatatgg aacaaaagta tatataccta aactgggtat gacttttgta gcagaagatt
     4081 gtggaggtgc aattaaaggt aatagaatag acatttttat gaacagtgaa ggaaaagctt
     4141 caaattgggg aaggaaaagt atagatatat atctacatta aattagtgaa tagaataact
     4201 ttacttgaag gacaaaaaag tataaaatat gaattaacta aactgttaga ttattcttaa
     4261 aacataaaaa tatatctaag gggggataag atgaaaatag tctgcttagg agacagttta
     4321 acgtatggat ttggagtatc acgaagtaat tcatggacaa acattgtaaa tgaagagact
     4381 cgattagaaa tagtaaataa gggtataaat ggtgatacaa caagtggtat gttagctagg
     4441 tttaatgaag atgttgtaaa aaactctcct gatatagttt ttataatggg tggcactaat
     4501 gattttattg ctggtgcggg aaatgaaatt attaattcta atataatggc tatggtgcat
     4561 cagtcatatt caaaaaatat tatacctata ataggtatac cattaaaacc agatattcca
     4621 aatgttaggg aagattggag ttgttttact gattttaata tagtatctca aaacttagaa
     4681 tcatatagtt attggataaa aaaattttgt atgacattta atactaattt cgcagatttt
     4741 tattcagaat ataataaaaa tatggaacta gaaggatata aaaaattata ttttgatggg
     4801 ttacatccaa ccaaagaggg tcatagaata atggcagaca tttttattaa ttcaattaat
     4861 aagtatatag aagagttata gattacatta aaatgcttgt ttatgcgtca gttgcaataa
     4921 atacatacag tagagagggg tttattatgg attatactaa aacttatgaa gagtggataa
     4981 agggttcata ttttgatgaa gatacaaagc tagagttgga gaatataaaa aataatgaaa
     5041 aagaaataga ggatagattc tataaggatt tagaatttgg gactgcgggt cttagaggaa
     5101 taattgaagc aggaacgaat agaataaata agtacacagt tagaagagct acttttggat
     5161 tggcaaacta tatattagaa aacacaacaa aagaagaaac aagtagagga gtagtaatag
     5221 ctcatgataa tagacacaag tctagacaat tctgtataga atctgcaaat actttggcag
     5281 catgtggtat aaaagcatat atatttgata gtttgagaac tactccagaa ttatcttttg
     5341 ctgttagaag cttaaatgca atcgctggta tagtcataac agcaagccat aatcctccag
     5401 aatataatgg atataaggta tactgggaag atggagcaca agttatgcca gagattgcaa
     5461 atgcaataac agagaaagta aatagtatac atgattatag tacaatacca accttaacgg
     5521 aagctaataa aaatttggta gttttgttgg acgaatcaca agatactaaa tttatagaag
     5581 cagtaaaaag ccaaattata agaaaagatt tagttaaaaa tgtaggtaaa agctttaaga
     5641 tagtttatac acctctttgt ggaacaggga atgttccaat aagaagagca ttgaaagaag
     5701 ttggttttga aaatataatt gtagttccag aagaagaaaa tccagaccca aattttgcag
     5761 ggcttgatta tccaaatcca gaagagaaaa aagctttaaa tagaggtata ttactcgcaa
     5821 aagaaaaagg agcagacctt gtaatagcaa ctgacccaga ttgtgataga gtaggtgtag
     5881 cagtaaaaac tactacaggt gagtatgctt tacttacagg aaatcaaatt ggtggtatgc
     5941 tgacacatta tataatagaa ggattaaaag aaaataacaa attaaaagaa aatcctacaa
     6001 taataaaaac catagttaca tcagaatttg gagcagatat agctaaagca aataatgttg
     6061 atgtattaaa tgttcttaca ggttttaaat ttattggtga aaaaataaaa ttatttgaac
     6121 aaaataaaaa tagaagttat gtatttggat atgaagaaag ttatggttac ttagttggga
     6181 ctcatgctag agataaagat ggtgtagttt cttcattgtt aatatctgaa atggcagcat
     6241 tttactattc aaaaggtata agtttatatg atggtttaat agaactttat aaaaagtatg
     6301 gatttttcaa agaacagact atatctttga ctttaaaagg tatagaaggt gtagaaaaga
     6361 taaaagaaat aatatcttat tttagagaaa atcaaattga ttatataaat agtattaaag
     6421 tagttgataa aaaagattat aaaaatggta tagataatct tcctaaatct aatgttttaa
     6481 aatacttttt ggaagatgaa tcttgggtag ctataagacc atctggaaca gaaccaaaac
     6541 ttaaatttta catagctgta aaaggagcta gtgatattga agcagataaa aagattcaag
     6601 gtttgaaaaa gtatatagat gacatggtcg aaaaattaaa ataaaatatt attattttat
     6661 aagataattt tgtaataata tttatttagc tgaaaattat aatttacttt cattgagtag
     6721 aatatggaaa tgttagtaga attgttattt tcagctttta tttgtgttat ttttacttaa
     6781 ataattatat tatagaatga atctgagtat aattaatatt gtataatgaa attaatttga
     6841 tagaaaaggg gatatttatg tttggtatag ataagaaatt gattgaatat ataattgcaa
     6901 aatccaatta cagtgaatta agttcaaagt cagcagaagt atctttcttc ttaatgttgt
     6961 ccatatttcc ttttttaata tttacaataa gttctatagc ttatataccg attcttcatt
     7021 taaataagta tatagctttg tttagaaata tgatgccaga aggtgctttt gctgtgttat
     7081 cttcaatcat agtttctgca atagacaata gaaacctgaa atttttagca gtcagttttg
     7141 tacttactat gtggacattt tcaagagcag taaaagctct tattaaagga atgaatagag
     7201 cctataaagt taaagaaact aggtcttttt ttaaaatttt atctatatca tttttattta
     7261 caataatgct tttggtcctt atttttttat ctatgatatt tttagtttac ggggaaaaaa
     7321 taggttattt tatttttaat cttgtaggat tggatgagat atttataaaa atatgggata
     7381 ttctaagata cactgtaggt ataataacaa taatagtgat atttacactt ttatataaat
     7441 acactccaaa taaaaagtta actattaaag aatctgcgcc aggtgctatt tttgctactt
     7501 ttgcttggtt tttagtatca tttttatatt cttattatac aaattactat gctaactatg
     7561 aagtaatcta tggaagtata gctgaaataa ttgtacttat gacctggatg tattttagta
     7621 gttggtctat cgttattggc tatgaggtaa attcaagatt gtattttaga aagataagac
     7681 atgaaatgtt aaagtaaagt atttaaaata aaagagagta tttagtatta taaatgtaat
     7741 tgaattaata aagaaatatt gacatttaag ttaaaaagtc tagaattttt ctgggctttt
     7801 tttattttta gtataacttg acaaatttta tttaatggga tagttttaat ttataatcat
     7861 ctttgaaaag atgactattg ataagtaagg tataaaattg gtaagtatca aaaaatatac
     7921 taaataaaaa ttatagtcct aatgagttaa tttctgtatt ttcaaataat gcagtattta
     7981 gtcatgtgtt tttatatgcg cctgtatttc caattaccaa tatattagaa taagctactg
     8041 taattagatt tagaaaaaag cgacataaat aattataaac aaataataaa ttacataaaa
     8101 ttaaatttat tcacaagtat atcttataaa atatgttata attaatagaa aattgtcata
     8161 taattaatta agtagtatta ttataaataa cattgaaagg tagtggttag aatatggata
     8221 tccataataa atatgtaaat tttataaaaa atatacctgt acctttttta tattgtagaa
     8281 ttgtaaagag acaagaagat atagaatatc gagtagaata tataagtaag ggtatgggca
     8341 aagtattaca attagaagaa ggtatttgtg ataaaaatat actagatgta ttgcctgtat
     8401 ttaagtctaa aaaatatttc aaagagttgt tctcaaatga agtggattgt ataaaaaggt
     8461 acattccgac acttaaaaac tggataaata taaaaaaaca gataataggg gattcataca
     8521 taattttgta ttttggtaaa attgtatttg attatagaca gataatcgat tcctttgata
     8581 aaaaggaaaa agtagcttat attaaagacg aagaaggcat atatatagat tgcagtgaaa
     8641 atttaatacc aatattaaat aacaacatta aaacaacgaa agatatcttt ggtaaaaatg
     8701 atatagaagt atggggagag aatacaggaa aattatttag agatgattat agaggaggag
     8761 tatctagtaa aaaaagattt ttacaaaatc tttttgaata tgaagaaaca ttttttatgg
     8821 ttgaaaaata ttttttgtat gatgaagacg aacttttagg tactataggg ataatagata
     8881 atataatata tagtggttac agtaatagaa attataactc aaaagattta atgaagatga
     8941 tagaacactc aattccagag aatatgtttt ataaagatgt gtatggaaat tatattggat
     9001 ttaattcagg ttttttgaat ttagcttgta tgaataaaga agaattatta gggaagaata
     9061 gttataaaat atcagaagaa gaagctttaa taaataagat atttgaaagt gataagggtg
     9121 tagttgaaaa taaaaaagta gttacatttg agttaaatgt aagtataaat gatgaaaata
     9181 agtgtattga gattacaaaa aggccgtttt ttgatagcta tggaagtgtt attggaataa
     9241 ttggaacagc aagggatata agtagaagaa aaagattaga agaggaaatg gataaaacta
     9301 ggatggaatt ttttgcaaat ttatcccatg aacttagaac gcctataaat ttaatatcat
     9361 cttcattaca agtgatagaa aaaaaagaag cagatttaat agaatcaaat gatactttaa
     9421 aaagaaattt aggtataata aaacaaaatg gaaatagaat actgaggtta gtaaataatg
     9481 ttatagactt tacaaaaatg caatcaggat atttagattt taaaccagaa gaatctgata
     9541 taattgcatt tatagaggag atatgtatgt ctgtagctga ttttgcaagc caaaataata
     9601 tacaacttac atttgataca gaaatagaag aattttcaat gttatttgat tcagaaaaat
     9661 tggaaagaat aatattaaat ttactttcta atggaattaa gtacaataag aaagatggaa
     9721 aaataaatat atttttatat gtgaaagata atgtatttaa tatgaaaata tctgatagtg
     9781 gaataggtat accaaaagaa aaaatagata aaatatttaa tagatttgaa cagattgaca
     9841 atgaactttc ctacagagtt aaaggaagtg gtattggact ttcattagtg aaatctttgg
     9901 ttgaattgca tgagggaagt atatctttaa aaagtcaact tggtattgga agtgaattta
     9961 tagtttcatt acctgttaga agtgaaaata atattgaaaa atataatcat aaaagagaaa
    10021 tcagcaatga actaagtaaa aaattagaaa tagagttttc ggatttataa gtttaaatat
    10081 aaaaaataaa attttaaaaa agtattgaca attgatttag aagggtttaa aataaattac
    10141 aacataataa ataaaaaggc gatgaatagg aagagtaaat ctaattcgat taccagagag
    10201 aagagtccat tggctggaag actttttaaa ttagagggat tgaaaactac ctaggagcta
    10261 ctttctgaag tcatctgcat tagtagattt tagtaagaat agtcgttagc tgcgttaaag
    10321 tgtaaagtga gttttcatga tatatgaaaa ctaaattagg tggaaccgcg ggaataatac
    10381 tctcgtcctt atgttataag gatgagagtt ttttgttgtt taaaaatata aacagtaatc
    10441 ataataatta tagaaaatat aaaagcgatg aataggaaga gtaaatttaa ttcgactatc
    10501 agagaggaaa gttcacaggc tgaaagactt tctaagtttg agaaattgaa aactacctag
    10561 gagctgtggt ctgaagttac tttaaaaagt tttagtaaga ctaaccgtta actgcgttaa
    10621 agtgtaaagt ggattttatc aataaatgat aaaatcaaat tgggtggaac cgcgagtata
    10681 gactcgtccc tttatatagg gatgagtcta tttttattgc aataaaagat tgaaaataaa
    10741 aaaaaatcgg aaaattataa atttaggagg aaaataaaat ggaaaataaa gtgaaaattg
    10801 gagtactagg atatggagtt gtaggaagtg gacttataga tataatagat aataataaag
    10861 aaaaaagaag tatagaaata gttggaattt tagttaataa tttggaaaaa cataaggata
    10921 aaaaatattc taatataata actaataata tagatgatat atttaataaa gatatagata
    10981 tacttgttga agtcatgggt ggtttagaac catcattatc ttatataaaa aaagcattaa
    11041 acaataaaat tcatgttgtt acagcaaata aagatttgtt agctgaatgt ggagatgagc
    11101 ttgcaaaatt agcaagtgaa aataaagtaa gtattaaatt tgaagcttca gtcgcaggag
    11161 gtattccagt attaaaacca ataatagaat cactagaagg aaataatata gatagtataa
    11221 atgcaatttt aaatggaact actaatttta tattatctaa aatgtatgat gagaatctat
    11281 cttatgatat ggcattaaga caagctcaag aattaggttt tgctgaagca aatccagagt
    11341 cagatgtatt agggtatgat gctgctagaa aactatcaat attatcaact ctagcttatg
    11401 acaatagagt atattggaag gatgtgtatt tagaaggaat tacagatata gatgaaaaag
    11461 atattgaata tgcaaaaaaa ttaaattgta aaataaaatt gattggtcaa agtaagtatg
    11521 aaaatgataa agtaagtgct tttgttagac ctgttttagt agaaaaagat aatatccttg
    11581 ctagaataga caatgagttc aatgctgtca tagtgaatgg agattcagta ggagaagttt
    11641 cttttgtagg taaaggagct ggaagtttag caacaggaag tgcggtttat tcagatgtta
    11701 tagatataat agataataga gtttcaagta tagattcttt tactaaagat aaaatacaag
    11761 ttaataaaat agttagagaa aagtgtggag cacttcttag atttaaaaaa tgtaataaag
    11821 atgaaattct aaatatagtt gagaattgtt tagtaaaatt cgatatttta aatgatgatg
    11881 atgaacttgc tattatggtt tatgctgatt ctgaatatga aataaacaac agtttatgtc
    11941 taataaaaga taaaggatat tgcgaaaaaa tgaataaaat gttgaaaata agttaaaaaa
    12001 gaaaaaataa gtttatatgt aacatatttt gacgatttta atactatata gtatgagatg
    12061 aaacaaaaca tctcataaaa aattaaaatt caagggagag atatgttata tggaaaataa
    12121 aaaatatgca aatggtggtt attcagaaga ctggtatgaa agaggagaat ctacagctaa
    12181 atggttccag aatgatagag aagaatatga aagagcagca tacgatgaag atagagaaag
    12241 aagaggctca aactgtggat gttcagattc aggaggaaat agacctagaa actgtgaaag
    12301 atttagaaga gaagctgaga taagagaaag agaagcaaga gaagcattct gtgaatcttc
    12361 agagagaaag aaagaagcac tagcatatga atgtgaagct agaaaattat gggaagaagc
    12421 agaaaaatac tgggatgaat attcaaaata caactataaa ggaatcgaat atttagcaga
    12481 agctgctaga ttatttgatg aaggtatgga atgtgaagct agaagaaatg gaaataatgg
    12541 aggaaacaat aataattgtt gccataaatg ccataaatgt aattgtaact gctgtagaaa
    12601 ataaaaaaat tcattgacaa atcaatagtt aaaatatatc atattatgta taataaagag
    12661 aaaattcaga aaaaaataat gattagaata aatttaagta tatttattag acaaatagtg
    12721 ctcagttagt gaggtgagta caatatgatt aaactcccat cctctagctt ttgatagagg
    12781 gtgggagttt ttatttattg atatatctta aatgaggata atattgtaaa ataaatgagt
    12841 atgagattgt aaaattagga ggtaaaaaat gaaaagtatt aaaacatcaa gtatgaaaga
    12901 aaaggtagat gaaaagatat taagagagca agtaagttct ataatagaag atataagaaa
    12961 taataaggat gttgcactta aaaaatataa tgagaagttt gatagaaata caagagatga
    13021 atttagaatt actaaggaag aaataaagga agcttataag catgtagatg acgaatttat
    13081 aaataactta aagattgctg ccaaaaatat aagagaattt gctaaagctc aaaaactaag
    13141 ttttgaaaac ccttttgaaa aagaaattta tccaggtgta atcttaggtc aaactaatat
    13201 tccaatagag tcatgtcttg catatgttcc aggaggtcag tatccattgt tttcaacagc
    13261 tcttatgctt ataatacctg caaaagtagc aggagtaaaa agaatagtgg catgttcacc
    13321 aactatgaaa aatactgaaa agataaatcc taaaacattg gtagctatgg atatagcagg
    13381 tgctgatgaa atatatgcaa ctggaggagt tcaagctata gcagcattta catatggaac
    13441 agaaaagatt aatccagttg acataattgt aggaccagga aataagtttg taactgaagc
    13501 taaaagacaa tgttatggac aagtaggaat tgattttgtc gctgggccaa gtgaagttct
    13561 tataatagcg gatgaaactt caaatccagt ttatattgca gctgatttat tagcacaatg
    13621 tgagcatgat ttaaatgcaa gaggaatatt attgacaaat agcttggaag tagcacaaaa
    13681 agttgaaaaa aatatagaaa gtatgctaaa agatttgcca acaaaggaca tagcatatag
    13741 ttcatgggaa aataatggag agataatatt ggttgatgat atggaagaag ccattaaaat
    13801 aagtaatttt tatgcaccag aacatttaga agttgcagta aatgagtata atgatatttg
    13861 tgatagatta actaactatg gttctttatt tattggaaat ctttctgctg aggtgtttgg
    13921 tgactatgtt tcaggtacca atcatacatt accaacactt aaagcatcaa gatatactgg
    13981 tggtgtttgg gttggaactt ttatcaaaac ttgtacaaag cagatattta atgaaaaagc
    14041 tatacaatca ttagcacctg tagctgaaaa gctggcaaaa gaagagggat tatatgcaca
    14101 tgcaaaggct gctgaggtta ggtttaaaaa gtagtaaaaa attttatttt ggcagtcgag
    14161 atttagtatt agactgagat aaagaaaacg ctgagaacct gaataaatac tatattttgt
    14221 gggcctcggc gtttctttta tatctgtata taataggata atactacctg tgtgtttaaa
    14281 tgctaactta atatttcttt tctaaataat gtttatgaaa tagttaatat ttttagttaa
    14341 tacttgaaaa aaacttttgc taactgtgca aattcatctg catttgtcaa tatagcaggg
    14401 tgtcctccct gactaaaaat gtgaatactt gcttttgaaa taaaagttgc catttgcttg
    14461 tactcttctt caagattatg acgacacatt tcgtcttcct tacttcctgt aaataaaatt
    14521 ggcatttcta atttacacaa ttctttatga aataaagggc gtttcttgtt tgcacattgc
    14581 aatagagctt ttgtatcaag attaacaaca ttctcccaat cttttccatg acaccattca
    14641 tagaattttc gtgcttgaat atcttgcttt gatttttccc ttccagtgat tagattatct
    14701 atgaagttat cattaagagt acgtccatca aagctatctg ctataattgc ttcaactaaa
    14761 tcgggacgtt ctaatccagt attaacagct gcccatgctc ctccacttgt tccaattaag
    14821 ccaactttac cacaattcaa atgttcaatc agtgctattg tctgtaaggc ttcatcatgc
    14881 catatgtcag gagaaaattg agaaactcta tcagactgtc cattacctaa aaaatcaatt
    14941 aagatacatc taaaattttc tacatataat ggcacaagtg ctttaaacat attagaagaa
    15001 ccagtattac catgcaggaa aattatcggt ttaccttgtc catattcttt ataaaaacaa
    15061 gaattacctt gatactgaaa atatgacata tattttcctc ctttttattt gttaagttaa
    15121 ttataacaat ataggtatga atgaaaaacc tcatgttctt tcttagaata aaaatgtgcc
    15181 acgcataaaa cgtgacacat ttctaattat aaattttgct gtttaagtat tctttgtatg
    15241 cttttctctg tcaaaaagta tttctctgat aactgcttga tactcattcc attttggaaa
    15301 tttatacaga tttgagaatt acgagaagct aagacttgtt tggtgtttgt gttttcaccc
    15361 caatgctttt tgtgctcttg ctttttggga atataaatca ctttaccatc tatatattct
    15421 tgcacttgct caatcagttc tttaggcagt aagtcaatcg aattttgata gctcattttg
    15481 cacctcctaa ataattactc tttaggatta gcaaaggcta ctgctactca caattaaaaa
    15541 taaaattttt tattatgtac aatagccttt gctatgcaca agttccaatt cccataaact
    15601 aaatactcct ttctggtttg aaaaattcat ctgaacattg atttcgattt taagatagag
    15661 ttcaatgaca aatttacatt tttattatat atattcagat agataattgc aactgtcttt
    15721 gtatgaggat attttattat agtcaatact ataatatata tttatatcat caaactaata
    15781 tgaacagttg aaaggaatat gaacaataaa acttagtcag gaagaataat tatgaagcag
    15841 acaattgaga aatttgattt taaagtttta ttgaaaattt attgacaaat ttaacttgta
    15901 aatattatac taaacttaat aaataaagtt tataaagtaa ggtgttgtct atgaaaaata
    15961 tagttggaaa accgcaaacg ataaaaaaag taaatgaaga tttaataaaa aacattataa
    16021 aaaatgaagg tcctataaca aaaccagaaa tttcaagcat tactgaattg agtttagcta
    16081 cagttaataa gattgtggag caattagctt tgaaaaatga agttaaagta agtggactaa
    16141 gtgaatctac tggaggaaga agagctcaat tatttgaaat aaatgcaaac ttagaacaca
    16201 taatagcttt atattattat agaaattgtt gtataggggt agttgccaat ttattaggtg
    16261 aaataattta tcaagaagaa tttaatataa gaatggatac atatgaaaac gttaaccaag
    16321 atactttttt tgttatagat actctaataa aaaaggaaaa aggaaacaat ataagggcta
    16381 ttggaattgg tgtcccagga gttgtaaaaa aaggtgtagt taaaaatata ccaactataa
    16441 aaagttggga aggtataaat ttagaaaatt taataaagag taagtacaat actaaagtgt
    16501 ttatagaaaa tgatacaaat attactacta ttgggattta tcaaaaacag tataaagaca
    16561 aatataaaaa tatggctctt atgtatttag aagatggtat tggttcaggt atagttataa
    16621 atggtgaatt gtatttaggt tctacaaatt ttgctggaga attaagttat ctaaatatag
    16681 aatttggtaa gtataactct aacaacttaa atcttgaaga ttatattatg agtttaagaa
    16741 aaagatattt agaaagtaaa gataataaat ttaagaaaga aattttatct ataatatcaa
    16801 actttataag gactcttgta tgtgtcctaa atccacaagc tatgattata cagtgtagta
    16861 tattaaccaa gagtgacatg gagtttatag aaaaagatgt aagtaataat gttggagaag
    16921 aaaattttcc taaattaata aaagtagagt cattgagaga aagaagtata aatggagtaa
    16981 ttagtatgtg tttaaaagaa actgactatc aatattcctt atctaataaa aaaggaggat
    17041 attaatgtga aatatttggt ttcaatagat ggagggggaa caaaaactaa attttgtgta
    17101 agtgacttag atggaaacat acttaaagaa cataccactg gttcaactaa ttataagtca
    17161 gttggaatta aaaaaacata tgaaaatatt aataatggac ttaaaaaaat attaaaagat
    17221 ttatatatcg attatgacga tattgagtac actgtatttg gtatatctgg ttgtgattct
    17281 cctaatgatt ataagattat aatggatgaa atattaaaaa ttgggattaa caaagaaaaa
    17341 atatatctgg caaatgatgc agttttagct ttttatgcac aagctgattc ccctggttta
    17401 gtaattgtgg ctgggacagg ttcaattata ctgggaatta aagaagatgg agaaatatat
    17461 agagttggtg gatgggggta taattttagt gatttgggtt ctggatatga tatagggaga
    17521 aaattattaa agaaagtatt gctttattgt gatgaatgtc atgagtatag tgatttattt
    17581 agttgtgtct tggatttctt tggagcaaat agttttgaac aacttgcata tatgattact
    17641 gatataaaca ataatgttga aatagcaaac ctagcttcat tagtgattga ttgtgccgaa
    17701 caaggtgata aattagctat tgaaatatta agagaatcat caactgaact atctaaatta
    17761 gctcaagtaa tattgagaaa aatatctaat ccaatgaaaa tagataaaag cgaaataaat
    17821 atagttttgt ctggtggtac attaaataaa actgtatatg ctcaaatgtt aatgaataat
    17881 ttaaaaacag aaaatacaga taaaaattta aaatttatat ctcaagaaaa tcagccagta
    17941 tatgggggga taagacttgc tatggcatta gctaatagga gagttaagca tggatagaaa
    18001 aaaggtaata gtacaaattt ttactggagg ttttaaggct aaagaaataa agtttaatga
    18061 tataaaatgt aaactactag aattattgga ctgtattgat atagaaaagg ttatcatggg
    18121 atggagtgta gatgaagaat tatatgtaga aactaaaaaa ctattaaata aatataatgt
    18181 tgaactatat ctttggttgc catgtttttc tgaaatagga ttacttgaaa aaagtaattt
    18241 actaattgat tatatgggga aggaagtcaa gggctatgct ttacaagagg atgaaaattt
    18301 tgaattttat tgcccaaata ataaggaaaa tataaaaaat ataaagaaaa tatatagtac
    18361 ttattttagt gatattaaat ttgatggaat ttttttagat aaaataaggt atggttcttt
    18421 ttcgaataaa ctaaatggtg tttttaattg tttttgtaaa gattgtttaa atatatatga
    18481 aaaaaataat attgataaaa agtcgttaat gagagaaatg aataatgtgt ctaagggagt
    18541 tatagataat gaagatactc catctggtat aacagaatac attaatggaa aatacaaatt
    18601 taaaaataaa atatgggaag atttttttga aacaaaaaat caaaatatat acaagtcatt
    18661 attagagata agtagttatt ttaaaagtaa gggattgaaa ataggtatag atactttttc
    18721 tccttttatt tcttacttta caggtcaaga tttaaaaaaa ttacaagaag tagcagattt
    18781 tataaaacca atgatgtata gaactacaaa agctccagct ggtttaccat ttgaatttga
    18841 aaggtttatt gatgaatcca tacataagaa taaagagatt ggaaaaagaa atttcttaga
    18901 tattttagga attaaagagt atgaaggaga tgtatatcct atagaatttg tactagagga
    18961 aatcgatttt atgacaagga atcttaataa agatatatat gtaggagttg aaataaataa
    19021 aaaggaaatt atagctcctg ttacacctaa gtacataagt gaaaatattt caaatataaa
    19081 taagacaaca gtaaaaggat atgttttatc ttgggatttg ttatcagcac caaaagaaaa
    19141 tattgatgaa attataaaat ggtttcggaa ttagtgaggt gatatagtga gtaagatatt
    19201 agaaatgcaa caaatatata aaagttttgg aaaggtagat gttttaaaag ggataaattt
    19261 tgagttagaa aagggagaag taagagctct tttgggagct aatggagcag gaaaatctac
    19321 acttattaaa attttaggag gagttcattc tcaaactaaa ggtaaagtaa ttttgactgg
    19381 taatgaaata atgtttaaaa attcacatca ttctaaagaa aatggtatta gtataattta
    19441 tcaagaatta agcttgatac caacgcttac agtacttgaa aatatttttc ttggtagaga
    19501 aattttaaaa ggaagtgtgt ttttaaacaa gaaagacatg cttgaggaat atatgaaaat
    19561 ttgtgaggat tttgatttta atatagaccc acatacagta gtttctaatt taagtgtagc
    19621 aaaccaacaa atggttgaga taatgaaagc tgtttcatgt aattctgaaa ttatcataat
    19681 ggatgaacct acaacatcac ttactaataa tgaaaaagat gggctattta aaataataag
    19741 tagactaaag tctaaaggta aaacaatagt ctatatttct cacatattag aggaagtttt
    19801 taaagtatgt gatagtgcaa gtataatgaa aaatggaatt atgattggaa cttatgatgt
    19861 agaggattta actaaattaa agataactca acttatgaca ggaaatgata atcacaaact
    19921 tattgagaag aaggattata catttgcaaa gtatgaagag ccaccagtac tagaagtaaa
    19981 gaatataagc tggtctaaca aaataaaaaa tgtttcattt aaagtttata gaggcgaagt
    20041 tgtaggtctt gctggtcttg taggttcaag aagaactgaa attataaatt tgatatttgg
    20101 tatagataat aaagatagtg gagacatttt tatcaatggt aaaaaagtag acataacttc
    20161 tcctaaggtt gctataaaaa acaaaatagg attgattcct gaagatagaa aaaatctagg
    20221 attaatttta gaacaagcta tatacacaaa ctcatcatca gtacaaattg ataaattcaa
    20281 gaaatttttg tttatagata gaaataaaga aattgagttt tctaaaaaag gtgttaagga
    20341 attaggaata aaagttagtg atgtaaagca agaagtaaaa gagcttagtg gtggaaatca
    20401 acaaaaagta gtagtttcta agtggttaga gaaagattta gatttaataa tatatgatga
    20461 gccgacaaag ggaatagata ttgctgcaaa agaagatatt ttcaatacaa tcaaagaatt
    20521 ttcaagtgaa ggaataggag taatatttat atcttctgat ttagaagaag taataagagt
    20581 aagtgataga attttagtaa ctagagaagg ttctattgta gatgatttaa aaaatgagaa
    20641 tatacaggtt caagatataa tgaacagtat atttaatgta taaaaaatga aaatggagga
    20701 ctgagatgaa gaaagctttt gagaagttaa atttaagaga ttatggagta ttattaggtt
    20761 ttttagcttt atgtatagct ataagtattg caactccatc atttttaggt aaacaaaata
    20821 tacttaatct tttacgacaa tcttctataa ttggaataat ttcagcaggt atgacttttg
    20881 ttataatatc tggaaacttt gatatatcag ttggagcagt agcagctctt gcaggtgcta
    20941 taacaatgaa atttgcgact ggtggaacta atttgtttgc atccatgctc ttaggtgtag
    21001 cgatttgtgc agtaatagga ttaatcaatg gtatagtagt tgcaaagata aatgtaccat
    21061 cattaatagc aactatggct atggtcacta ttgtaagagg attgctgttg atgcttacag
    21121 gaggatatcc aattactgaa aatataccaa tacttgatta cattggaaat ggatatttgt
    21181 ttaatatacc aataccagta gtaattttct ttatagtggt gttagtttca tttattgtac
    21241 ttaataaaac taagtttggt agatatgttt attctgtagg agggaatcaa gaagcaagta
    21301 aacttaatgg tataaatgta gattctcaca aagtgaaagt gtttattatt aatgctgtac
    21361 ttgcaggaat agctggaatc gtactaacag gaagacttgg gactgcaact gctattgcag
    21421 gtgaaggata tgatatggat gcaattgcat ctgtagtaat aggaggtaca tcagtggcag
    21481 gaggttctgg ttctgtactt aaaactgtta taggagtatt acttatgagt gttataaaca
    21541 acagttttaa tcttcttgga atagatgtat atttccagta catatttaaa ggattaataa
    21601 ttttagcagc agttggattt gattcttata gcaagaaaaa acttgcttca agataaaaaa
    21661 taagggggat ttttttatga aaaaattaat gaaacattta gcattgttgt tatcacttgt
    21721 tatgatattt ggtttagttg gttgttcaaa tggtggtgac aaagaaaaaa atggagaaaa
    21781 gaaaatagca gtattactac caggttcaac aggttacttt gtagcaacta aacaaggtat
    21841 agatgcaaaa tctaaagaat taggtgtaag cgtggaatat gcagatgctc aatgggatgc
    21901 tagtaaacaa ttatcacaag ctgaagattt tatggctaaa ggggtagaca tgattgcatt
    21961 atgtggagtt gattctgctg taagtgagag aatagttaaa gctgctaatg attcagacgt
    22021 ccctatagta gcatttacaa attctatagg aagtaaccca actggagaat ttaaaggttt
    22081 agttacatat ataggacaaa atgaggagga aacaggagca ctaactggaa aaattgctaa
    22141 aaacttatta ggagaaactg gtgggaaagc tgtattaata gaaggagtac caggaactac
    22201 tccacaagta ttaagaaaaa aaggtcttga aaaagaatta aaagatagta atatagaaat
    22261 agtttataat caaacttcaa attgggagaa agagcaagct ttaaaagtaa cagaagattt
    22321 aattcaaaag aaaactgatt ttaatgttat aatatgtcaa gatgataatt ctgctacagg
    22381 tgcaggtcaa gctttaaaag atgctggtct taaggataaa gttaaagtca taggtttggg
    22441 tggaagtaaa gatggattaa aagctataac tgatgggtta atagatggta caacttatat
    22501 gtctgctgta gaagaaggtg gattagtcat agaaaaggca tctaaattcc ttaaaggtga
    22561 aaaaatagaa cctgttacac aaattaaaca agttgaagtt aacaaagata atatttcaga
    22621 gttcaagggt gaatggtaaa ttgtcataga taataaataa gatttttaga gttactttag
    22681 aaactatata aagtaactct ttttttaatt taatttagtg ataaattatt gaatagtatt
    22741 tctaaacaaa taaaaaatta atataaacaa agacttaatt atactaaatt ataagtaaaa
    22801 ctaatataaa cttatggaaa aaaggaaaac gtctaaagta ttggaattac tatagtgtag
    22861 gtgtggtatt aaaaaaacat aaaaaaacat aaaaaaattg ttgaaaaaaa tatagaaatt
    22921 ttgtataatt aaagatttga aaagttcggt atacaaagcc aattcttttg tatataatat
    22981 atataaattc agaaaaagga agggatatgt atggccaggc ggttattttt agaaaggatt
    23041 ggacttatat ttttaggttc agcaatatta gcatttggag tttataattt ttattattta
    23101 aacaacataa cagaaggtgg agtgttaggt attttactac tgttaaaaaa tttattcaat
    23161 atacaaccag caatagcaaa tgttgtaata gatggtttac tacttttagt aggatataaa
    23221 tttttcggta aaaaattcct tatatattca atagtagctt ctataacttt ttcagtgtta
    23281 tatgacttat ttgaagctat aggacctcta gtaccacaat cacaaaatat gcttttatca
    23341 actattttgg caggtgtaac tgttggaact ggtgtaggta tagtagtaaa agcaggttgt
    23401 gcatcaggag gagacgatgc tttagcatta gtaatttcta agactacatc tttgaacata
    23461 gggcaagttt atttagcaac agatgttatt gtattgttgt tatcattatt ttatctatca
    23521 gcgtttgata tcttttattc tttgattgct gttacaatta gtggcaaggt aattgatttt
    23581 atttattatc atggtaaaag tttggatatg gatataagta atgatatagt tccagaatgt
    23641 taatataaat taaataactt tagattttat taggggtggc agatttgcca ccttttttgt
    23701 gtctaaatct ttatagaaag tataatccca tctaggtttt tgcaaatctc aaatatagaa
    23761 ttatgttgga atgactggga atacgattgc tagatgtgca ttttaaaaac ccaattttat
    23821 atttatgaaa tactaatcat taatggatgt ttcttaatcc tatctttata cctaaaatga
    23881 taagataaat gaagtagcaa tacaaaacta aaatttgtaa aagagattaa attaaatgga
    23941 ggagctaaaa tgttggatat agttatgata gcattgttat tcataggatt tgtttccatg
    24001 aagttacttg ctgattggtg tgggaaacaa actgagaaaa aataatgtaa tgtatggagg
    24061 gattacctat gtggtttctg gcagttgtaa ttataatgtt aataatatat ttggtatatg
    24121 cgcttattaa tcctgaaaaa ttttaaacag tataaggcta ataagtaagt tgaattgggg
    24181 ggaacaattt ttatggtgca gatagtaata gtgttagcga tttttatgat tttagtaata
    24241 cctatgggca agtatttata tcatatagct actaatcaaa aaacatttgg agatagacta
    24301 tttgataaag tggacaattt tatatataag gtatgtagta tagataagaa aaaagaaatg
    24361 aactggaaac agtatgcatt agctttatta tttacaaatg cagttatggt atttatagga
    24421 tatatcatac ttagaactca aagtatgcat atttttaatc caagtggaat taaaagtatg
    24481 gaacaaggac tatcatttaa tacaataata agttttatga caaatacaaa tcttcaacat
    24541 tattcaggtg aatcaggact ttcatacttt agtcagatga cagtgataat atatatgatg
    24601 tttacatcag cagcaacagg atatgcggct gctatggcat ttgtaagagg tctagtcggt
    24661 aaaaagaaaa cattgggaaa tttttatgta gatttaatta ggataacaac aagagtactt
    24721 ttaccaggag ctttaattat tgggcttata ttagttacac aaggagtacc acaaactttt
    24781 gctgggacag aaacagtgac tactatagaa ggaaagcttc aagatatagc tagaggtcct
    24841 gtggcagctc ttgaatcaat aaaacattta ggaacaaatg gtggtggatt ctttggttca
    24901 aattcatcac acccatttga aaatccaaca ataatatcaa atatagtaga aatattatct
    24961 atgatgatat taccaggagc ttgtgttgtt gcattcggtc acatgataaa gaataaaaaa
    25021 caaggttggg ttgtgtttgg cgcaatgtca atcatatttt taataggtct agtagtgtgc
    25081 tttaaagcag aaagtgcagg aaaccctatt ttatcacaat tagggcttaa tcaaagtatg
    25141 ggaagtatgg aaggtaagga agttagattt ggtatagcac aatcatcatt attcacaaca
    25201 gtgactactt cctttacgac aggtacagtt aataatatgc atgatacact gactccttta
    25261 ggaggattag taccactttt aaatatgatg ctaaatgtag tatttggtgg taaaggcgta
    25321 ggtctgatga atatgttgat gtacgctata atagcagtat tcttatgtgg attaatggta
    25381 ggaagaacac cagagttttt aactaagaaa atcgaaggaa aagaaatgaa gcttattgct
    25441 ttacttatta tattacatcc acttttaata ctaatgttct ctggtctttc agtagcaata
    25501 ccagcagggc ttgaaggaat ttcaaatcct ggattccatg gattatcaca ggtattgtat
    25561 gagtttgcat cttcagcagc caataatggt tcaggatttg aaggtctagg agataacact
    25621 atgttttgga atataacaac aggtatagtt atgttctttg gaagatatgt gtctattata
    25681 gtactactag caatatcaag tttgttagca tctaaaaaag ctgtaaatga aagtatagga
    25741 acacttagaa cggataattt tacatttaca atagttttag tacttgttgt tttaattgtt
    25801 ggtgctttaa catttttccc agccttagcg cttggaccta tttcggagca tctagtgttg
    25861 tggcactaaa aaattgggag ggagtaaagt tttatggaaa ctaaaaaaac aaagtttatt
    25921 acaaaagata ttttcaaaac ttccattata gaagctttta aaaaattaaa tcctaaatat
    25981 atgatgaaaa atccagttat gtttgttgta gaaattggat ttatagtaac attgatactt
    26041 acaatagtac ctagtttgtt tggtgatgaa ggcaataatt taagagctta taatgggatt
    26101 gtaacaataa tcttatttat aacagtcctt tttgcaaatt ttgctgaagc tgtagctgag
    26161 ggaagaggta aagctcaagc agatacactt aagaaaacta gaaaagatac agtagcaagg
    26221 gttatagata cgttaggaaa tgaaaaaatg ataagtgcaa gtgagctaaa acttggtgat
    26281 attgttttag taaatgttgg agaagttatc ccaaatgatg gagaagttat agagggcata
    26341 gcttctgtgg atgagtcagc aataactgga gaatctgcac ctgtcatgag agaatcaggt
    26401 ggagatttcg catctgttac aggtggtact acagttgtaa gtgactggtt aaagataaga
    26461 ataacaacaa aaccaggaga atcgttctta gataaaatga tagctcttgt agaaggtgct
    26521 tcaagacaaa aaacgccaaa tgagattgct ttaaatactt tgttagtagg gttaacactt
    26581 atatttatgg ttgttttaat atctctttat ccaatggcaa actatgcagg tgtaaagatt
    26641 ccaatttcta ctatgatagc tcttttggta tgtttaatac caactactat aggtggattg
    26701 ttgtcagcta ttggtattgc aggtatggat agagtaacta gatttaatgt aattgcaatg
    26761 tcaggaaaag cagtagaagc ttgtggagac gttgatacta tgattttgga taagacaggt
    26821 acgattactt atgggaatag acttgcatca gaatttatag cagttggaaa tgcagattca
    26881 aaagatttaa taaattattc tgttatgtgt tcattaaaag atgatacacc agaaggaaaa
    26941 tctatagttg atttaggtat gaaattaggt tcaacaggta agacaaaaga ggcagaggaa
    27001 gctgagtttg tagaattttc tgcacaaact agaatgagtg gtattaactt agctgatggt
    27061 agagctataa gaaaaggtgc ttatgatagc ataataaaaa gagtaaaaga atctggtggt
    27121 acagtgccag aagatttaga agaaaatgta aatcgagtag ctaaactagg aggaacacct
    27181 ttagtagtgt gtgtagataa tgagatatat ggaattatct acttaaaaga tacagtaaaa
    27241 tcagggcttg tagaaagatt tgctagactt cgagagatag gaataaagac aataatgtgt
    27301 acaggggata atcccttgac agcagctaca attgcaagag aagctggagt agatggattt
    27361 attgcagaat gtaaaccaga agataaaata gatgctataa aaaaagaaca attagagggt
    27421 aaaatagttg caatgacagg tgatggaaca aatgatgctc ctgcacttgc tcaggcagat
    27481 gtaggtcttg caatgaatag tggtacaaca tctgcaaaag aagcagcaaa tatggtggat
    27541 ttagattcag acccaactaa gatattggaa gttgttgaaa taggaaaaca gcttttaata
    27601 actcgtgggg ctttgacaac atttagtata gcaaatgatg ttgcaaaata ttttgcgatt
    27661 ataccggcta tatttactct tgcaatacct caaatggaga tattaaatgt aatgaaactt
    27721 tctacatcat ttagtgcgat attatcagct ttaatattta atgctataat tatcccatgt
    27781 ttaatacctc ttgcaatgaa gggagtaaaa tatagaccta tgaaatcaca agctatgtta
    27841 atgagaaata tgcttatatt tggtcttggt ggagttatag taccattttt aggaataaaa
    27901 ttaattgatt tattgattac acctttagta atgattctta atttaggtta ataaatggag
    27961 gatataaaaa tgaaaacatt aaagccagca ttacttgtaa gtattgtatt gttagttgtt
    28021 tgtggacttg tatatccact tgttttgaca ggtgtaagcc aagttgcatt taaagataaa
    28081 gccaatggaa gtatgataga agtaaatggt gtaaaagtag gttcagaact tattggtcaa
    28141 agctttacag atgcgagatt ctttaaggga agagtatcat cagtaaacta taatacttat
    28201 acaaaagaag atttagtacc agataaagat ggaaatgcat cttatggagg tgtatcatca
    28261 gggtctttta attatggagc tacaaatcca gaacttcatg atagagtaca aaaagatata
    28321 gaaaagtttt taaaggataa tccaactgtc aaaagagagg atataccaac agatttactt
    28381 acagcatcag gttcaggact tgaccctaat ataagtccag cctctgcaaa aattcagata
    28441 ccagctatag caaaagcgtc tggaatatca gaaagtaaat tacaaaaaat tgtagatgat
    28501 aatacttcca aaaaactttt tggagtttta ggtgaagata gagttaatgt actaaaggtt
    28561 aatatagagg tcgcaaaaat attaggtctt atatagtatt ttgtggaatt ttagtaacaa
    28621 aatattactc aagatttaat ttaaaggtta aattttaaag agtgaaaatc aataatgatt
    28681 ttcacttttt tatttgaata ttactaatat ttggaataaa gtgattgtaa gtataaaatc
    28741 caaaaaaagg ttgcattatc aaaatgataa tactataata tatttatcgt tatcaatttg
    28801 ataatagatt tggacatata ggaggaaata gtatgttata taataacgca ttagagttga
    28861 taggaaacac acctgtagta aggctgaata atttaggctg tactaatggg tgttctaaca
    28921 tatatgtaaa attagaaaaa gtaaatccag caggaagtat aaaagataga gctgtttatg
    28981 aaatgttaga gggcttagag caaagaggag aattaaaaaa gggagatgta ctagttgaag
    29041 caactagtgg aaatactggt attgcattat ctatgatagg aaaattaaaa ggatatgaag
    29101 tagtaatcgt aatgcccgaa acaatgagcg ttgagagaag agatttaatg aaggcttatg
    29161 gagcaaattt ggtgcttaca gatggtaaag ctggaatggc aggttccata gaaaaagcaa
    29221 atgaactttt aaaagaaaat ccaaactata aaagcttaaa acaatttgaa aataaagata
    29281 atccaaatgc acattataaa acaacagcag ttgaaattat gaatgatgtc aaagatttag
    29341 atatattcgt atgtggagtt ggaacaggtg gtactttaat aggaactgca agatatttaa
    29401 aagaacaaaa tccaaacata agagtaattg cacttgaacc aaaaaaatct ccagctatat
    29461 cagaaaacaa gactggacct cataaaatac aaggaatagg tgctaacttt gttcctgaaa
    29521 attatgattc tagtgtagtt gatgaagtaa tattagtaga tgatgaagat gcatttgata
    29581 cagttaaatt acttgcagca aaagagggaa ttttagtagg gatatcttct ggagcaaata
    29641 tatttggtgc tatagagatg gcaaaaaagt atccagataa aaagatagtt acagtagctc
    29701 cagatggagt tgataagtat atgtctatgg gaatatttta ataattttat ttgagggagt
    29761 gaatactttg tttaagaaaa taaataaaga tattgaatat ataatgaaga atgatccagc
    29821 agctaggtct aagatagaag tttttttact ttatccatca gttcatgcaa tgataatgca
    29881 taggatggct catgctcttt ataagaagaa gaagcttttt acagcaagat taatatccca
    29941 gatatccaga tttatgactg gcatagaaat tcatcctggt gctaaaatgg gggaaggtat
    30001 attgatagac catggaatgg gtgttgtaat tggagagaca gctgaagtag gtaatagggt
    30061 gacaatatat caaggagcta ctttaggagc tacaggaaaa gatactggaa agagacatcc
    30121 aactgttggt gatgatgtat taattggtgc tggaacaaaa atactaggac ctttaaatat
    30181 aggttcaaat tcaaaaattg gtgcaaattc tgttgtcgtt aaggatgtac ctaatggtgc
    30241 aacagttgtt ggtataccag caaaaatagt aaaaataaga aacctagaac ctgtaaaaaa
    30301 gaataaaaaa gaagtgtcct atgagtatga tgaattagat aatgt
//
LOCUS       DAEPOG010000027        29908 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEPOG010000027 DAEPOG010000000
VERSION     DAEPOG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.027"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(69..329)
                     /locus_tag="KR432_001069"
     CDS             complement(69..329)
                     /locus_tag="KR432_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639620.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(592..945)
                     /locus_tag="KR432_001070"
     CDS             complement(592..945)
                     /locus_tag="KR432_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639621.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(962..6139)
                     /locus_tag="KR432_001071"
     CDS             complement(962..6139)
                     /locus_tag="KR432_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBH0639622.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6161..7147)
                     /locus_tag="KR432_001072"
     CDS             complement(6161..7147)
                     /locus_tag="KR432_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0639623.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7148..7846)
                     /locus_tag="KR432_001073"
     CDS             complement(7148..7846)
                     /locus_tag="KR432_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH0639624.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7843..8895)
                     /locus_tag="KR432_001074"
     CDS             complement(7843..8895)
                     /locus_tag="KR432_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0639625.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(8888..9316)
                     /locus_tag="KR432_001075"
     CDS             complement(8888..9316)
                     /locus_tag="KR432_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0639626.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9316..9642)
                     /locus_tag="KR432_001076"
     CDS             complement(9316..9642)
                     /locus_tag="KR432_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH0639627.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9658..11187)
                     /locus_tag="KR432_001077"
     CDS             complement(9658..11187)
                     /locus_tag="KR432_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0639628.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11469..11891)
                     /locus_tag="KR432_001078"
     CDS             complement(11469..11891)
                     /locus_tag="KR432_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639629.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(11891..14344)
                     /locus_tag="KR432_001079"
     CDS             complement(11891..14344)
                     /locus_tag="KR432_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639630.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14344..14511)
                     /locus_tag="KR432_001080"
     CDS             complement(14344..14511)
                     /locus_tag="KR432_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639631.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14538..14984)
                     /locus_tag="KR432_001081"
     CDS             complement(14538..14984)
                     /locus_tag="KR432_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0639632.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15098..15526)
                     /locus_tag="KR432_001082"
     CDS             complement(15098..15526)
                     /locus_tag="KR432_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0639633.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15540..16604)
                     /locus_tag="KR432_001083"
     CDS             complement(15540..16604)
                     /locus_tag="KR432_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH0639634.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16609..17052)
                     /locus_tag="KR432_001084"
     CDS             complement(16609..17052)
                     /locus_tag="KR432_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639635.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17301..17741)
                     /locus_tag="KR432_001085"
     CDS             complement(17301..17741)
                     /locus_tag="KR432_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639636.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17846..18043)
                     /locus_tag="KR432_001086"
     CDS             complement(17846..18043)
                     /locus_tag="KR432_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639637.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18212..18532
                     /locus_tag="KR432_001087"
     CDS             18212..18532
                     /locus_tag="KR432_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0639638.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18614..19120
                     /locus_tag="KR432_001088"
     CDS             18614..19120
                     /locus_tag="KR432_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639639.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19821..20576
                     /locus_tag="KR432_001089"
     CDS             19821..20576
                     /locus_tag="KR432_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0639640.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20719..21147)
                     /locus_tag="KR432_001090"
     CDS             complement(20719..21147)
                     /locus_tag="KR432_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0639641.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21568..21768)
                     /locus_tag="KR432_001091"
     CDS             complement(21568..21768)
                     /locus_tag="KR432_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0639642.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(21951..23120)
                     /locus_tag="KR432_001092"
     CDS             complement(21951..23120)
                     /locus_tag="KR432_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0639643.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23356..24177
                     /locus_tag="KR432_001093"
     CDS             23356..24177
                     /locus_tag="KR432_001093"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBH0639644.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24253..25641)
                     /locus_tag="KR432_001094"
     CDS             complement(24253..25641)
                     /locus_tag="KR432_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBH0639645.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25785..26543)
                     /locus_tag="KR432_001095"
     CDS             complement(25785..26543)
                     /locus_tag="KR432_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0639646.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26545..27261)
                     /locus_tag="KR432_001096"
     CDS             complement(26545..27261)
                     /locus_tag="KR432_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0639647.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(27263..27970)
                     /locus_tag="KR432_001097"
     CDS             complement(27263..27970)
                     /locus_tag="KR432_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0639648.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(28087..28623)
                     /locus_tag="KR432_001098"
     CDS             complement(28087..28623)
                     /locus_tag="KR432_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0639649.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28725..29795)
                     /locus_tag="KR432_001099"
     CDS             complement(28725..29795)
                     /locus_tag="KR432_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0639650.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
BASE COUNT         9457 a         5218 c         3159 g        12074 t
ORIGIN      
        1 gcactatcta caatactact ttatcttttc ctaataatat ataatctggt gctactgcta
       61 ctttcatgtt atacaccgtt ttgtaacata ccttttatat cagtaacatc ttccttaatt
      121 tcctctacat ctgttttcat tattcccatt tgaacaagta tatttttgtt tatttcttgt
      181 tgttgtgtag ataattctat aaaattttct acagtttttt tatacatgtc tctatcttct
      241 tttttctcct gcatagtatt tttaaataat agagcacata gtatacctat tgcccccaga
      301 ctactcaatt cggttattaa tttatccatt atagcctcct attgtctaaa ttttttatac
      361 ttatagcttt aatttttgtt atattcttac atcatgcaat attatatttt caggattatc
      421 aatttaatct tctgtacttt aaattgtagg tttaatttat agattgaata aataataaat
      481 atatatacaa tttctaatat aacaaaaaat aagtacaagg cttacttatt aaagttaaga
      541 ccttgtactt tacataaaaa aaataactaa tacatagtta ttctaaattt actattcaac
      601 tggtatttct aattctttac atctaattct aacttgctct ctaattactg ctggtacttc
      661 ttctattgtt tttctgcctc ttaaaattaa agctacatat acttctacca ttccactctc
      721 acctccagat tttcttacac taaaagtgct tatattactt gcttcattga cagaactttg
      781 acttaacaac atctcatata tttctgcatt tgctataaaa ttatttgtta tatcatcatc
      841 ttgctttgct tgacctatac ttatgtctga aatgtcttta tactgcattt ctacttttct
      901 accttgtttg atttgttcta ttttattttt atcgaattta aacaattttt acacctccat
      961 tttaagcttg tggctcgttg ttttcagcat catatctaaa aatcatagat ggacgtttta
     1021 tagctgtcca tttatctcct ttttctacta tgattttagg ctgagcactt tttatgttgg
     1081 cttcttctaa ttcatagatt tcttgataac ttgagtctga ttttttgatt tcaactagtt
     1141 tcatgtcttt gaataagtca gatttattta tttttacttg aactttagtt ttatttatta
     1201 attgatatat agggtctcca agatttaaat cttcttcaag tcctctatct agtgtaattg
     1261 taatatcttg ttcattagat gttgtattaa ctaccttgta cattctatta cctatcataa
     1321 cataagaatc tttaattata gaatttccag tactctcttt aaaaataaat aaagatgcat
     1381 ataaatcatc acctttacaa taagctctaa atagtatctt attctctcct attttaattt
     1441 tatctggtgg tacttcaaag gatatttttt cagtagttaa atcacttaca gattttacac
     1501 tttcaccatt tataagcatt tctattctac ttatatcttt attagggtct tgtattttta
     1561 tcttaattga tgaaattgat ttttcagtaa tttctatagc agcatttgtc gttcctactt
     1621 ctgatataat atataatgta ttgtctgttg caagtacatc ctttactgta tttacatctt
     1681 cagaaatagg tactatagta aattctttta aaaattctac tggttgttca attttatata
     1741 catccctatt ttttccagta acccatactg aattatcatt tttaagtact aatatatggt
     1801 cacttccata agattttatt tctcttatgt ctgttagtgc ttttgtaaag aacttaatta
     1861 tagtattttc actatcgctt cctaatccta aagctccctt atcattatat ccttgtacaa
     1921 ataattcatt acctgtagtt actactaatg tatagttttt accacaatgt acttctttac
     1981 agctagatat agttgtaatt ctttgtaatt cagtaactgg attattgtga cctataccta
     2041 attgatattc ggaattttta ccaatagacc aaagactatc atcagatttt ataaaataag
     2101 taaagttatc tccacatgaa acatgcttta catctgatac attaatctgc acaaattcat
     2161 ccacttgatt tatagacaag tcttgcaata attctccatg gtagtttgta ccacatccat
     2221 acattgttcc atcattaagt aatataataa tatgagtctt agaaatagat acttctttta
     2281 tatcttcata tggaagctca gtataaaggt ctcctttgta taaatttcct aatactaaac
     2341 cataatctat gtcttttgtg gtaggtatca ctatcttatt gtttagaagt ataaagttat
     2401 gtctgttttt aatagatgtc aaatcaccca ttacaaaatt catatgctta taattaaata
     2461 ttctattatc attatttcta ctatcgtatc tatttccaac acctaattgt ccatagttat
     2521 ttaatccaca aatatatata tcattagtat cttttattgc tatatttgca tattctaaag
     2581 gaagtatttt ctttactctt gtattggttt ttataaatgt atctcttttt gttacatcac
     2641 caacacctaa ttgtccatta ttattaaaac ctgtaccata taggtctcca ttatcactta
     2701 cataatgtcc acatctataa ttcattccaa tatcctttgc ctttgatgct atttttttaa
     2761 tcttctctgg agtctcactt cctgtacctg aaccattact acatttgcct gcagtatata
     2821 catccccttc acttgtaagt aaaagtactg cattatttct gtttggagct acttttacta
     2881 catttgaaat tggaatttca gaaaatgaac tttgattgct attactattt agacctaagc
     2941 aataatcagt atttaaacct gtactatatg cagtattata gattgttatt atataggtat
     3001 ttatttcatt tggtataact tcttttacat tttccataca ctttataaaa ctatactggt
     3061 cagtagtatt tcccatacct agttgtgaat tattattgta tccacaacaa tatgcaatgt
     3121 ttgtgtcctt tacaataaat gttgttgtat ctgagcagta cacatctctt acattagtta
     3181 tattgtttac agtaaatgtc aatacgtctg gatggttgct ttcgcttcct aagcccagtt
     3241 gcccatgagt gttttctcca catgaatata atgtgttatc atctttgatt gcatgcgagt
     3301 gagtagtccc taaaacaaca tctcttacat tttggatgtt tactttggta aatgtatttc
     3361 tatttactat atcacctaat cccagttgtc ctttggtatt taatcctgtg gagtatagtg
     3421 ttccatcatt tctaattgca aatgttgtgt taccatctat acttgttgat attttcttta
     3481 tattatcaag gtttatctga gtgaacttat ttctatttat tgtgtcacct aatcctaatt
     3541 gtccagaact attatttcct gtggcatagg ctgttccatc attttttact ataaacattg
     3601 tatttcttga tgttgtaatt tcttttatat catccataaa tataaatgag tttgattgtt
     3661 cagttatatt tgtgaatttt tttactggtt cgttgttata ttcccctatc ccaagtttgt
     3721 tattctgatt cattcctgtt acatatatat tgttattttt atctactact actacagtat
     3781 ttccttttac aattatatct tttatgtctt ttaatcctgt atcatagtta cttgtgccat
     3841 ctatatttgt tgaattccca aattgcccac tattagcaat cccttttcca tataacttgg
     3901 aattactatg atatattata aaattacttc ctaaattaga actctcagaa ttaatactag
     3961 tataacttct atgtccagta atggttgagc caaatgtttt tggatatcca gtaatatatg
     4021 aacttccttt cttaaaaaat aatatattat ttgacatccc tattactttt tcaattccac
     4081 taaaatcagt ctcttgtata aaataagtat tatgatttga atttgtagcc tcaataaaag
     4141 ttccatatct tgaataatca gatatacttg gactgctatt taatgttctt aaatatccat
     4201 gatatcctgt taaataaaca taacctcctt cgtcaattat tgcacaacca tcataaaaag
     4261 gaaaaacatt agttatttta ggtaacgttc taagtccatt tgatgtagaa gatgttgaat
     4321 ttgttctaca ttgagatact aatgctttgc tttggttact attatttcct ttacctaatt
     4381 gataattata attgtatcca gaaacatata gtttcccttc actgtttatc atatatacag
     4441 aataatcata tgctgctatc ttttcaatat tacttattcc ctgcttatat ggataattag
     4501 aagttttttc tatattatta taaggattat ttccccatcc atatacttct tttgagtgat
     4561 ttaaagctat gacataattg cttcctacac aaaaatcctt tacattctct atttctattt
     4621 ttgtaaatat atttctatta acttcatctc ttaaacccaa ttgtccatta ctatttaaac
     4681 cacatgaata taatgttcca ttattcttaa gcacaaaagt tgagtttccg tttcctttta
     4741 tatccaaaac attatctata tccaccttag tcataactga cctgttaaca gtatctccca
     4801 atcctaactg tccataatta tttaaaccac aagcatacaa tgtaccatca ttcataacaa
     4861 taaacatatt gctatctgtt aactctatct tcttataatc ttgaaaatta tcacttaact
     4921 ttacatttaa aaattcacta ttgtcctttg taacatataa ataatcatca ttaaccaata
     4981 aacctgaata attatctaaa tttgaagatt gcacatttga aaactcatac agaaaagttg
     5041 tatgaaaaaa tgaaccaagt tgacctgcta aattacagcc agatacaaat atctcttttg
     5101 aaccatttat taaaaaatta aattgagaac cacatttaat atcttttata ttttctacat
     5161 taaccttagc aaaagtattt cttgatgcta catctttatt ggcattggct agttgaccat
     5221 aattgttttg tcctgtacta aacagtgtat tatcgtattt taatatcata gtatgattat
     5281 ttccacaaga agcatactta acattattta tactagaaag agtaaataca attgaattat
     5341 tattatttcc tgtaccaagc tgaccaactc cattatagcc tgtagtatac atagttccat
     5401 cattcttaat aagaattgta tgactactac cacacgctat ctgttttaca ttatccatac
     5461 cttctatttt attaaataca cttttattta aagtactatt aattcccaac tgcccatttg
     5521 aatttaatcc acatgcatgt gctgtcccat ccatttttat gataaataca gattgattat
     5581 aagtcactac atctttcaca ctatctatat ttactttagt aaaagtagct ctattgttag
     5641 tatcacctag tccaagttga ccacttgagt ttaaacctgc tgaatataat gttccatcat
     5701 ttttaagcac aaatgtaaag tcataaccac atgcaacagt ttttacatca tctattggta
     5761 gcttagtaaa ttcataatat actgtagact caattacacc taattgacct acattacttg
     5821 ttcctgctcc atatgcaaat ccatctttag ttactgctac actgtgtttt gaaccacaag
     5881 atacatattt tacattatca atttttactt tactaaacat ccttctctct gacgaatctt
     5941 ttccaagtcc tagttgaaaa cctgtattat cgccacaagc atataaatta ccatctgatt
     6001 ttaaaccaaa ggtactatta cttataccag taaaaaattc tacaaaatca taatcattat
     6061 atcttttatc aaaatcatca atcaatatgt tcttatcatt aaaataagca ccacgctgta
     6121 aaagtttatt ttgcttcatt attagtacta cctcctttat ttatcttatt tcatatgaat
     6181 atttacttag agttatatct ttttgttctt ttaaataagt gttactagat tttgtctcaa
     6241 caaagttatt atccatatca tgtatatatc taactctatt tgcactaaat ggtaatacta
     6301 catctctaaa aatcacatta ccaatttcac ctgttataca ttttctattt aaatctaact
     6361 taagcccttc tatagctact atgttatttc catttgctaa tgtatcatac caataaccag
     6421 cctcatctac agttacttct gactctaatt ctaggtatat taatatatct agcacttttt
     6481 cagataattt gttatagcta ttttttaata ctgatatatc agtttgtatt tgaccactta
     6541 taacatcatc aaaagatata ttttctattt ttttaactac ctcatttata gctcctgata
     6601 aatctttaga atctgtagtt aatatatctg tatctccaat ttttactttt atttctttta
     6661 tatttttatc aaacttatca tctaaatctt gaatatcctt ttgagttaca aaaaccaacg
     6721 tcgggtctac ttctaatgta acatttgaag tattggacaa ttgtaaaatc atttttacaa
     6781 ccaaatcttt aatactgcca tcttcagcac gtggcttata cgtctctgga tacttaccta
     6841 tagctattat attattctca ttatccagaa cagcagcttc tcttatcata aatccaccaa
     6901 ctgttcctgg taaaatagtc tctataacta tccaattagg atttttttca tcaattctta
     6961 gagaatttat atttccttcc caaactacat tctttaaagc agtttgttcc tctgttggct
     7021 cataatattc tccattacta tctccaactt gaaacttggt taatccaaca agctctccta
     7081 gtgcagttgc atttgctatt ttttctctac caattttggt taatattata taaaattttt
     7141 cttccaatta taccacctcc gatttaggat aaactactac attttctacg cctgtatcat
     7201 ttgcaatagc tatattaatc ttacaattat tttcaatgca tttaatgctt aaaggataaa
     7261 cctttatttc ttctctactt aacattccag ctccacagtt tacaacagca ctacatttta
     7321 taattggttc aaagtatgga tgaactttta cctcttcact acataccata cctccaccac
     7381 aatagaccat acttttatct agtattattg gttctaaata gtgtaataag tgagcaggtt
     7441 ttactctttc aatcatctcg ctacaatcta aaagagtttt atagtcacag ttatttgcta
     7501 taaaactcaa tacgaatgta aattcatcac tataaacttt tatatctgtt tctgattttg
     7561 tatatgcctc acatatactt tttataactt caatagtact agtacctctg cttctcattt
     7621 tagcttttat attgctacgt cttgtgtcaa aatcaagttc ttttttttca atgcagagta
     7681 tgtcttccca catatctaat ccccatgtcg ctgtatcaac aaataattgg ttaaaggtat
     7741 catcaatatc ttctttaaga atatttaatt ctgtatcgta tgcaccttgt atctcctcta
     7801 caatgtaatt tctatcaaat gatggtagtt tatcaattag cttcattgat tttccacctc
     7861 actaaaatta acagttgtta cactaggtat tttatcttca ttaatagtta tattatctgt
     7921 acttccattt ataagtagat tacttaaatc gtgtacacca gtagtattta taagtattcc
     7981 catgactttt gtatagatta tctctcctct aatatctcta aagtatgtat ttatactttc
     8041 taggaaagat tcttttacat tgtctaatgt atatccatct tctagtttca ttactgcact
     8101 tatacttatt tctattggta atggtgtcac aactgttata gttggtccaa taggcttctc
     8161 ttcatctata tgttgctgac acctttcaat cgtttctgta tcaacagctt gattattttc
     8221 cccaaatatc aagaccttaa ctgttcctgg accatcccat cttggataaa ccttaacatt
     8281 atagactcca tctacttcca aagcccattc ttcatagtga gctttatttc cacttgtagc
     8341 ttgatttctt tggattttat aaaatctttc ttttagttct tcatctgtct ctatatctgt
     8401 accaccttca aaacttcttg tgttagtaat ctttgttact ccagatatat tatcaactag
     8461 tttaaattca caatttgtag gtaaattata tttcttacca acttccagag cttgaactgg
     8521 gctattgtca ccttcttcac taccaatagt tacatctttt attactacaa atagtaaatc
     8581 tctatatgat attattgtgc cattatttat tacagttcct ttctctccaa taaattcaac
     8641 ttctccattt gactctgtac ctaactttct atatacacca aattcattga ctcttttatc
     8701 aagaaactgg ttatatgtgt cttgtataaa agccatttta tgtatctttg aaagttctag
     8761 atatatcttc gaaagttcta gattatttcc agataccatg ttgtttagaa aagaaccttc
     8821 tcctttataa atatcaagat taatattttc aagagttcta ttttttatta cttcatatgt
     8881 ctggtcacta tacatttatt tccacctccc catagatagt ggatactttt acatttgcac
     8941 tcaatacgtc tctgttaaag ttagcacttt ttacgtttac ttctaatata tatggattta
     9001 ttagaagggc ctcttttatg aatctactag cttcactttc tgtaagacct tttgtatatt
     9061 tttgtcctat tagttctgaa agctctgtcc catattctaa agagtatatc tcatgttcat
     9121 atctatttgt ctttatacac ttgtatatcc aaacttttaa ggcttctttt ttttcaatta
     9181 ttttaaagtc acctttttct aaaataggtt catctttttc aaaatcccat gccacttcac
     9241 gaaagattgg caattcttct gttttaggta agatataatc ctctgggaca cctataaaag
     9301 gaaatattgt actcattata agctcaccaa cttacttaca acagcaaatt tttcgcctat
     9361 tctaaacatt attaccatat ccccagattc aaagttatct ataaaaggat tttttacttc
     9421 atgctggtgt tcttgatttg tttctgtgtc aaatgtttct agctgtctat caatcatcca
     9481 actatctatc aaaatatctt ctttttctaa tatgatgtta tttgtctcta tttttaaatc
     9541 tggtaattta cttttaattt ttccaataaa aaaagaaggt tgattatgat actttccttc
     9601 ttctcttatt attcctataa attcatttat tggattagcc attatatcac catcctttta
     9661 taatactctc ctagctgtat taaaatctct tctactactt aacttactta ttttaactac
     9721 atcacctttt tgtggagcat gtaaaaattc tccattacct atatataatc caacatggct
     9781 gactggatta tgaaaaaata ctaaatctcc tgcttgaaga tttttttgtt ctactttctt
     9841 gcctttttta gattgttgat ttgacgttct tggcaaacta acattaactt ttttaaaaca
     9901 gtagaccatt aaaccagaac agtcaaaact acttggtcca tttccacccc atttataagg
     9961 tttacctaga tgtttttttg cctctgatat tactatttta gctttatcag atacattacc
    10021 agaataacca ccaataataa tttttccttt tcttcttcca aatttactag cttcttcaac
    10081 atttccaaat aaaatatcta tatggtatgt tccatttttt tctatagtta ttgcaggtcc
    10141 tacgtcattt actttataga ctccatcacg accagaaact cctgtaatta cctgtatttc
    10201 atctccatag ttcaataaag gatgtttatt taaaaactct tttgtataat aagtttgctc
    10261 atatttacca accataggag cagcgcaagt ttttttagat gggtcaagtt ttttctttct
    10321 acaatctgta tctccacctt cttcttttct aggacagtaa gctgtaaatt ctgctgtaaa
    10381 ctcttttcct cctgcataat cttctccccc taaattactt tcttccttag gttcatcctg
    10441 tcctgctgac ttttcatcca taagattttg aaaattaagt tcaagctcaa tttgatattc
    10501 tccattttgc caagtatgtt tgtctgtatc tatataaaat agtcctacaa gctttgtata
    10561 agaatcttta acttttactc ctctaccagt tatacaactt acatctccat aacctttaag
    10621 agaacagctt ttttctatcc cattaaactc gctatcaata tctacatctt gattttcttg
    10681 ttgctgaatt actttttgca ttattacatt tacttcctta aaaatttcat tatctatttt
    10741 ttcgctaatc ttgcttccat actggtctac cactattact ttatttttta cattttccat
    10801 gctctccgaa aaggtggtat ttataatatt aaatccctct tcaaacataa cacttaatgt
    10861 aacagttcct ttttcaataa cattaaactt atctaaatta gcctctatca tatacttttt
    10921 ctttgtcttt ttacttgcct ctgtatatgc actcattatt gtgtcataac catttactcc
    10981 tataaacatc tttgtatact tgacattggt ctttgctatt gttccaactg aaagcctatt
    11041 ttcagcaaat acttgctttg ctatgtcttc aactaactta tctttaaaat tgtatgacac
    11101 ttcactttgt gtaagtaaaa atcccatatc tttagataca aaactaattt cattactgct
    11161 tgaatcttta gacctattaa ttatcattcc tctaaagagt tctttctcat ctacatagaa
    11221 acagactgta ctagctattg gtatatcgat ttgttggaaa tttacatcaa atgatgattg
    11281 tattattgaa aactctagtg tccttgatgg agatttataa tcacctgacc atgatacctt
    11341 gtctactatg tcagttatgt catatatact tccgtttttt atgtgtaccc aaatcttaat
    11401 aataaaaaat cacctgcctt ttcatttaat tctatcagca gttacttaca acttattatc
    11461 tataatagtt attgttttgg agttactttt ggtatcttta ttcttttata ctcttttaaa
    11521 tctaatgtaa aatatacatc tcgagtccca tctctttctt cataactaaa cccttcaatt
    11581 atgacttcca tatttatatt tgtttccgta attgtaaatc ttaatataaa accttccttc
    11641 atccattttt ctatcttatt tacacaatca tatggtgatg gaaaacctgt atagtcacag
    11701 aaagtgtagt ctttatttgg aaaaaaactt gatattgaaa tgcttttaag agctacacca
    11761 ccaaaagttg ctacttcacc tattttgagt atactagaag agtttacagc agcttttcca
    11821 ttaatactaa aggaagatgg aaatactgga aatctaaatc tatcttctgc ttgtctaagc
    11881 cacatttcca ttataagaca ccccctaact gtgccaattg gatacttgca actaattttg
    11941 atgttatctt ttctatatca gcttcttctc taatcactat tgtatcagct aacttagcta
    12001 tgtttatatt tccaccattt tttctgcttc tatattgatt tgcttcttgt ttagttaaaa
    12061 ccatttcacc ttcatggagt ctggtttgat agtcattata aggaacatag ttcaatccaa
    12121 atgcattatt acctttctta cttgaataag ctttaccaga atcaccagcc tttgttccag
    12181 catatggatt atctttgtaa ttttttttac tcttttccac tagatttaaa actgttcctg
    12241 tgatgcttcc attttttatc ttattaatca tagtttgcca ccatttgcat atatctttaa
    12301 ctattttaca tactttatct aatatgttaa aaaaagctcc caatattggc tctataattt
    12361 tccatccagt ttccaacaga ggtcctaagg tctcccatac agttttccaa ataacaccca
    12421 gtgtctctat aaaattgttt atttgaggtg catattcacc taaaaaagta aaaattcttg
    12481 tgataatagg actaacagct tcaactacag gtccgattag ttggaatgct gtagttacta
    12541 ttgtaccaaa tatacttatt aatccttgtg caattggtga tttattgttt atactatcta
    12601 gtaaattatt tactaagttt actactggaa gtagtgaatc tattaatgct gtaaaatcaa
    12661 tattttgtaa tagtgtactt cctgcgttaa caattgcttt tggtgcattc cctatcattg
    12721 ttgctgcatg attttcaaaa cgttcttgct tattttcaac catcatttta cgcttttcag
    12781 cctcattaga aattcgatat tgagtagaat cagaaaactc ttttccattt tttacacttc
    12841 tagattgcat ctggtcatat ttgttgccaa taattatctt accattcatt acattgccta
    12901 ttttaccagc cattttttca gataagccag ctaaattccc taatccactt tctataaaag
    12961 tttttatttt ctttagtcct tgacctaaaa tatcatcaaa aggtttaaca atactcttca
    13021 aaccattgtt aatgcttgtt tgagctcctg atattatacc tggtattgta ttactcattt
    13081 ctttagctaa ccccatctgc tttacactta tgccttccaa tgcttcatca aaagttttat
    13141 aattttctcc taccagagta ttaagagatt ccatatttcc ttttgctgca cttaagaaag
    13201 ctgctgatac atcttgttca ctacttgtat tcatatttaa agctctaaca tctaccatag
    13261 cttgagttat atctttttct tttttgccac cagtaatatt actaatcatt ttagctttag
    13321 ttcccattgc aacaacatct tcagggtcaa aaggcgtatc atttgccata tttactaaac
    13381 tgtcataata cttatcaaca tctttttggc tagtttttcc agtatttctt ttcattacac
    13441 tttctagaac aactttctga ttttgttcct gaccaagtaa gtcaacaccc tttttcacag
    13501 cgacacctat agtagcacca tttgtcaaat cagatatctt cccttttatc gaaccaagta
    13561 ttccacttgc catatccttt acagctattg caccactcca taccttacct gcaaacaatc
    13621 tcaatttggg aaatacttta cttataatgc tacttgccat atcttttata gctattacac
    13681 cactccaaaa agtattttta aacaacatta acttaggcaa aattctagcc atagcactac
    13741 ttaccatatc tttaagatta atacttcctt gataaggacg tttctctact cgttttaatt
    13801 ttcccaagat actacttatc actctcttag tcatatcttt tgcttttatt acgatattcc
    13861 aaatcttgcc accaatcttt tttaaatttc caagtaattt atttttagtt ttttctactt
    13921 catcactttt agctttaaat attaatataa tcataatatt tgaattaaac tcatttttct
    13981 tttgaatcaa cttattaaaa taatcaataa tcatagctat agttattgct agtctcataa
    14041 tacttccatt taaacttcca ataactcctg aaacagcatt tatgctagaa cttaattttg
    14101 ccatagttga acttacacta ctagcagtag tttgaacttt tcttaacgag gaatttaaac
    14161 tgtttactct actacttgca cttccaatag aattttttat gctactagcc attacattag
    14221 tggaaagtga aacagccaca gttgctctcc taaaattatc agttactgat ataatttggt
    14281 tcatttttgg tgtgtaatta tctgtaagat acatatttat accttcttct ctcacatttc
    14341 ccattacata ccacctcctg tcaaaggaca cactccatat ccatgtttca ttttttcaac
    14401 ttcttcctca atttctaagg cgaaaaaagc ttcaataagc tttaattcgc ctttatccat
    14461 tgcataaaaa agggacggtc ttataccttt tttcttccaa taatagtaca ttattgtagt
    14521 cttggtatcc gtccctatta gttttttacc tcttctatca ctgcattttt accataaccc
    14581 ataacatctt gaagtattct atataggttg gtaatttcac ctggtaataa tagttttctt
    14641 gccaattctt ttggtgttgg agccttaaat ttatccatta gttccttatt tttaaataaa
    14701 agctttccat ccaaatcaca aacaccttct ataattgtaa gcatctgcat ctccggtaag
    14761 tctatatccg ttttcatatt ttcatcaatt ttaagacaag tattttgtat ctcttcatct
    14821 ttacttattg ttaaagctct acacattact ttaaattcct gcccaaatat agttgataat
    14881 ctcttaagtt caactatagt acttggtctt tctaagtttt ctgtatctgc atttaaaagt
    14941 aaatctacta tgtttatatt ttttgataat ccattttcat tcattattat ttgctccttt
    15001 atcaatcaaa ttcaatcaac taactttttt acttattaac tacctaaatt tattaagtat
    15061 atatctacta aatatcagta taaaaccaaa tataaaacta aattatatca agaaaatcat
    15121 actcagtaaa tgtgaaaggt gcttctatct ctccttttac tccaacctcc caatcagcta
    15181 aagttaaatc atcaaatgct atatttttta ctgctattct ttctgctcca taagaatctg
    15241 ggtcatttaa tttagatata gcaacaaatc taggttcaga acctctcttt atcttttctc
    15301 caattaactt gtgcattctt gaactaacat ggtatagagt tattgaacct tttcctttat
    15361 atcccatata ctttgtatca gtacccattt gacctgctat tataatatcc tcttttgtaa
    15421 attccatctt tgcttgaaac ttctttactt ctgctacttt gtttccatca agccaaagtt
    15481 ctccccaagt accactcatt acatttctag cttccatatt tgccatatta ataatcctcc
    15541 tatatttcta ttgataaatc tatatcttcc atagcatcaa gtacttttat ttttgctttt
    15601 aaaaatactt tagaacctgt gttagcttct tttatttctt gtaatgtcat ataagataaa
    15661 tctactcctt ttgattttaa atacgatttt tgtgcttcaa aatctatttc aacagtagaa
    15721 tcagattcta taagtgctga cttttctaat tcttctaaat aactttttat agctactatc
    15781 aataaacatt tgttgtcata actgttagta acctttccta tatagtcatc tattatcacc
    15841 tttcttatgt cactatgtat aatatctaaa gtgtcaacta tttttatttt ctggaacatt
    15901 tctccttttt cttctgttaa ctcagttaaa gaatttactc ctcttgcaat tcttatagcc
    15961 cctgcttcct taataagtat aagctctcct ttattaaccc ttgattctgc atcaactttc
    16021 gtcatcttag gtatatcaac tacatcacta agcttagtat aagttactga ttgacttaaa
    16081 ggtgttcctg ctataagtcc agccacccta cttgtaaact catcaacact gtatttcttt
    16141 tctccaacta acacatcttc tgtagtaaaa ttaattatcc cttcatgatt tcctacaact
    16201 tttcctaata cagctttaac cttaacctta tctatatctc taagtttaat tatccaattt
    16261 tttatagcag tcttatcagc ttctactgct tttggcatac atagataatt aaattcctta
    16321 gtctctaaaa aatctaatgc agtttgaata tctgcttctc cttctattac ataaactaat
    16381 aatttatttg gagtgttaac atttcccatc aaagctaaat ttatatactc tttattttca
    16441 gcagataaat caactggtat atcctctttt tcatgtattt cattaagacc tagtgcctta
    16501 gcatctttaa gcaccattgc aattattcct ctagctgaac gttctttaac agttgtagct
    16561 agctccttaa atgatatgtt gatacttggt aatcctatag ccatactatc actctcctaa
    16621 atttaaattg atttcttcca tattttcata tactacatct tcttcatata cactatccag
    16681 ataatttagt gtaattgtaa agttcaatac ataaattgac tcttcttttt taatactttg
    16741 ttctagcttt gttatattta attttctgtc tgttaccttt atatttctgt tgaataactt
    16801 atttaattca tctgaaattt catacattct aatactcttt gactgtggtt ttggtaagta
    16861 ttttatagag ataacaatat ctttattatt cgtatttaac attataggtg tacaaataac
    16921 tgatagtata cttacaaaaa aacaagaccc ttcagagcct tgcacacttt catcatcaat
    16981 aaaaatactt tcagtaaagt tttttttgag aatctcaata attgtttcta atatttcctt
    17041 gtatttaagc aaataatcac cacactatca ctaaatattc ttgcttaatc tgataatctt
    17101 actataaatg atataagtca tttttaaata tacatctcat ttattcctgt caaatatctt
    17161 ctatattttt aggtaatatt attcttttca atttatcacc tccttcaata tggatttctt
    17221 tcacaataac attatctcac atattttatt gcctagtgtt gcaatagtgt atataaagtg
    17281 ttgtaaagtt gtcatatttt ttataatatc tcactcaatt taaatatcat ttttttcttt
    17341 aaattgtagc aagatgttct atctaagtgt agtttcatag aaatatatgt catattgttt
    17401 tttgtcttac tattataaaa aagattaaaa aattccattt ctatatcatt tagacatgtt
    17461 agagcatttt ctatcttctc tttttctatt tctatatctt ctttttccat ttttaatttt
    17521 cttaatcttt cctctctttt taacacctca ttttccacag aagaacttat gttatatgtg
    17581 ttactagtct tttctgtata taccattgct ccacatccag agtattcatt ctcacatctt
    17641 tttatgtcca aattaatatt ctttatcttt atatccaaat acttataatt atgtaatctt
    17701 tcatctgttt ctttaaataa tatatctttt ttgttttcca tagtacttct ccttttttat
    17761 atacttatta ttagcttcaa atttacaata atctctttag tgcacagttt tttactaatc
    17821 tcacaacttt caagaacata ttgttctata actgtataaa aaaaacttct tctatatttt
    17881 gttctaaaat actagaaatc ttcttagcca taagaagacc aggatttttt atacctcttt
    17941 ctatttgaga tatatagtca tttgtaattc cagcctttct ggcaagttga atttgtgtca
    18001 tacctttgca ttttctataa tatttcaagt tatttttaaa cattgtttta tacctccatt
    18061 accattaaga atattttgtt cttccacact tagattatat agaatcattt gttctttatc
    18121 aatagatttt agaaaaaaat attcttttat attttattag ttttattttt tagaacaatt
    18181 tgttataatc tatacataag gtggtgttat aatgtttgcc aaaagattaa gagaacttag
    18241 aaaggaattt ggattgactc aaagagaact tggagaaaaa gtaggcgttt cccaaagagt
    18301 cttagggtac tatgaaacag aaaacagatt tcctgatgaa cacattttaa acaaattggc
    18361 tgatgtattt aatgtatcag ttgattacct tcttggaaga accttagtta aggaaaatat
    18421 tgatacagta gctgcacata gaaaaaatcc acatgaagag ttacctgaag aagcacaaga
    18481 acaacttaat gattatatag aatttttact aaataaatat aaaaaaaaat aaaccatatg
    18541 atgagcagtc tatttttctg ctcttttaat ataatttaaa ggcaaacata cattctattt
    18601 tagggggatt tttatgaata atttggataa gctttttgaa ttagcttctc aagaagagat
    18661 aataattcat tataccactt atattgcagg tgatttagaa gggctatata taaacaaaca
    18721 tggtattaaa attatatcat tactcagtaa tttaaaacaa aactcaaaaa aattgacatc
    18781 catcttagct gaagaacttg gacatcattt cactagcctt ggatactatg tatcctctta
    18841 taacgattat tatacaaaaa ttattataga taagtgcgaa aataaagcct taaaatgggc
    18901 ttgtgagttc ttaattactg aagaagatat aataaacata attaattctg gcatcacttg
    18961 cgtttatgaa atggcagaca tacttaatgt ggatattact ttctttcaaa aaagattaga
    19021 atttttatca ttaaaaaaac agtctttaca acttggaaat aataaatatt taatattaac
    19081 taatttgccg tatttctaca tatttgatcc aatttcataa taaaaatagt agtaatttta
    19141 actggcttaa cagtaaaatc tatatattta ttttaacaaa ttttggtttc tataaattca
    19201 atattaatat ataataatga tatcatatta tttaaaattc taaaaacaaa tatatgcttt
    19261 tgttaagaaa ggttgtttga tgaatgtata aagaaaagtt gtttgataat tattttaagt
    19321 tcttagcact attattttgg ccaattatgt ggtataagtg gatagtaatt tcaaatggta
    19381 ctcttgaaaa tatgttattt accatatatg caatcgtcgc aattattttc atcattttat
    19441 attcggtgtt tatgataaaa tataaagaca ttacacaaat agatttcttt tacaggataa
    19501 gtactctact tgcttttata tttacacttt ttagcttttt aatataccca aagagtttat
    19561 ttttcttgta tttaaagatt atttttactg gaatatacct ttattattca atagtaaaaa
    19621 ctcttaaatt taaagatgat gaaggtgtag tgggtattat gagctcactt ttattaattg
    19681 taataacttt gttctactaa atttaattct atttgactta atatggcttt aaatattaga
    19741 catctacatt attttctgat attatttaag atggtagtct tatattaata atgaaataca
    19801 ctatgaagga gttaactatt atggaaaaaa aagttttagc aacagttgga gaaaaagaga
    19861 taactaatat tgatattgaa aatgcattaa aatcattaga cccttatcaa gctatgcaat
    19921 ttaaaactga agaaggaaaa aaacacttat taaatgattt agtgaatcaa gaattattct
    19981 tcttagatgc taaagaagaa aaattagatg aagaagaaat atttaaatta gaaatgaaaa
    20041 agatagaaga aaatgtatta aagcaatttg ctataaataa agttttatct agtgttaatg
    20101 taacagaaga tgaaaaagta aaattctttg aagctaataa gagctctttc tctaaaccag
    20161 aaagtgcaac tgctaaacat atattagtag atagtgatga aaaagctaaa gaaatacttg
    20221 ctcaaataaa atcagaagaa atatcttttg aagatgcagc tcttaaacat tcttcttgcc
    20281 catctaaaga tatgggtggt gatttaggaa cattcggtag aggacaaatg gttcctgaat
    20341 ttgaagaagc tgtgttctct atggctaaag gtgaagtaag tgaaccagtt aaaactcaat
    20401 ttggatacca tataataaaa ttagaagacc ttcaagaaag tacagagtct acatttgatg
    20461 aagttaaggc tgaagttgaa aaaagtttat tatatcaaaa acaaaatgaa gtttatggaa
    20521 ataagataaa tgctcttaat gctaagtacg gaaaccttgt aaaatacaac gattaatcat
    20581 aaggtcatat ataaaaaagc tgtcttttca caatatttat tagaaaagac agctttttat
    20641 attaaagttt aaatattcag tttcatatta tcattctttt attgtaaaga actcagctta
    20701 aaagtttaaa taagattttt aattatcatt agctaaattt ttatatccac gtattatctc
    20761 ataacagtaa cctattacat ctcttctttt tattatccca ataaatgtgt cttggtcatc
    20821 tataacagga atgaaatttt gatttaaaga ttttataact aaatcttcaa tatctgcgtt
    20881 gatggaaaca ggagaattat ccatttttct atttatatct gtcactggaa catcttccaa
    20941 tccctttaag tccagattta aatcgttttt taatgtccat aaaaaatcac cttctgttat
    21001 agttccaaca tatttaccat ccttacttat tattggaatt gcagagtatt tgtgatattc
    21061 cattttttct agtgcttgcc ttatagtgta gtcttcatat atatatgcta cttcactttt
    21121 tggtgttaga aagaataata tgttcattac actacccacc gttactgaaa acttataaat
    21181 attactcaat atttataatg ttactttttt gcagtaactt tctcctttct tattttctaa
    21241 gtattcttaa tactatacgc tgtaacaagc gtttttcctt tgctcatttt aatcttatta
    21301 tgctttatta atatttttat atggtctatc agtatcactt attctctttt tagaaaattt
    21361 actaaaaatt aattgactta aacttagcca taataatttt actctacact attaatttta
    21421 acatatttat ctcattatta atgattttat tttaataatt ttaatttatt ttgctattaa
    21481 ttttatttac aaaaactgaa aaaaccgcta aatgattagc ggcttatctc tcatattact
    21541 agaacactaa tatatatgaa tttttaatta taatctaact acgttagcag cttgaggacc
    21601 tttagctcca tctactactt cgaactcaac gctttgtcct tcttctaatg ttttgtatcc
    21661 atctccagtt atagctgaga aatgtacgaa aacatctcct tcaccttcta cagatatgaa
    21721 tccaaatcct ttttcgttgt taaaccattt aactacacca gttttcatta ataaatcctc
    21781 ctaaaaaaac acaaatattt agctactctt ttctgtaacc ttattactac tctatcatat
    21841 atagctatgt ttgtcaatta ttattatttt tatacattaa taatcaattt ttttacttta
    21901 taattttaaa tgattattat cttttacact ttatttttct ttataatttt ttaataattt
    21961 atttttatta tatggaatct tatggactta tcactccagc ctaaatccca tggcaccaac
    22021 attctatcag tattatggca agttaccaaa ggatatcctc ttgagtctaa tcctgtcact
    22081 gtcgaaacat gtgttattct gcctttcttc tcataaccaa caaaatcccc cggacgtaaa
    22141 ttataagctt ctttgtatac ttcttcatag gaacctttag caattaatga acctcttcca
    22201 ctatacagtg cataattttt aaaagcttgt gcatttaccc atgcttttgt acctgctctc
    22261 tcgctataat tccaaattga gttctttttg aaccttccac tttcaaacat tatctgtgat
    22321 gcaaagtttg cacaatctcc accttctgga ttataatccc tatagtttgg attaaatttc
    22381 ataccatgtt cttcatcagc tgcggctcca cagtatttat gtgcataatc tatagccttt
    22441 ttttgttcat ctgtaagctg taactctaca ccatcttgtt gcccaatata gtttcttata
    22501 tcctcagatt ttatattttc taaatttaaa gagtctgcaa aagggtctgt gtaccactct
    22561 ttagttatga catattgatt atctataatt tttacattta gataatgata tgtacctatt
    22621 ctaaaggcat tttctttgtc cttttgattt tcatatgaat acttatattc agtcgatact
    22681 gaacagatta taccatagag gtctttttct ttttctttta tttttttaat cttaaccctt
    22741 gtctttatgt ctttaaattt tacaccttgc ttactagacc agttttctaa atacttcatc
    22801 ttttttactt cattttcata agcccacaga ccaaattttt tatccgtctc ataaagacct
    22861 tttaaaatgt tttcattttt ctctaatatt gccttatttc tgtagtcaaa taagttttca
    22921 agcaatagtt tatattggtc ttttacatca tcaccttcaa catttgcaga ttccatgact
    22981 ttttcttcca tatttttcat acttaaatca ttgattaaaa atacacctgc tgtagtagct
    23041 aaagacaaaa atacaaatgc cattatacct ttgatgtact tattcttatt tttcagtatt
    23101 ttcaaagcta cacctcccaa atagtacacc ttataaatga aattacaaag aagattctgt
    23161 catatacaat aattttcata tttaataatt ctctatttta ttatgtccaa tattaaaaat
    23221 taattgtatc aatacactgt tttttttaca attttttatt ttataatcta cttttttctt
    23281 ttttttatag taatatatat gtatataaat attttggtat cattataatt aatacatact
    23341 aatggagggt tttttatgca aaaaaaagtc gcagcaatta atgacttatc tggaatagga
    23401 aaatgctcat tatcggttgc tatacctata ctttcagcat taaaggttca atgttgtcca
    23461 tttccaacgg ccatactttc aagtcaaact ggttatcctg aatttacttt cttagacttt
    23521 actgatgaaa tggtaaaata ttcaaatgtt tggaaaaatt taaaagtcaa ttttgatagt
    23581 atatatagtg gttttttggg ttcaaagcat caaattgaga tagtggctaa ttttataaat
    23641 gattatccca atgcttttat cgtagttgac cctgtgatgg gtgataatgg agttatgtat
    23701 ccaatattca cagaagaaat gagacaggaa attaaagaat tagttaaaca ttcagattta
    23761 actactccta atttaacaga agcttgtttc ttaactggaa atgattatac taaaagcgat
    23821 tataatagag atgagcttat atatattgca aaatctgtat ctgatttggg acctagcaaa
    23881 gtcgttatta ctggtatatt agaagatgat aatatattaa atttggctta tgatagagat
    23941 aatgaccatg tattctttac atcagtcaaa tacaataatt gttcttacag tggaactgga
    24001 gatatattca catctatact ttgtggtatg ttagtaaata aacatgattt gggagtagcg
    24061 gttaatactg caacagattt tatatataaa actataaatt acacatctca atttgatact
    24121 gatagaaatg atggagttat gtttgaaaac tttttatcag atttaactaa tatataaaat
    24181 caaaaaaact atccatttta taaataccta taaatcataa aatttaggta tttacaaaat
    24241 ggatattatt tactaaacta ttggtatttc aattgttacc atagcaccac cagttttcac
    24301 agaattttca atatataaaa ttccattatg tttttgtaca atattattta caatataaag
    24361 tcccattccg taatgagatt tagaatttct actcaaatct cctgtataaa actgttctgt
    24421 agcttttgac aaatctgctt tagaaaaacc acttccacaa tctgtaacta caaattttaa
    24481 gtatttatta cttactgata cacttatgta tagtttactc tggcttggtg aataatctat
    24541 agcatttgaa attatattca taatagctct gaataacagt tctctatcta tagttatatt
    24601 tttaggcaaa ttatcttgtt tataaaactc cacatctaag tttttagtac tagctaatgc
    24661 ttctatttgc agtaagacat cttttataaa ctcactcata ttaattgctt ctttatttaa
    24721 tatatagcca ttctctgttt ttgatatatc tattaaagta ttaatatatt tttctatttg
    24781 agtgacaccc tttgagatat attccatgta ttctaataac tcaggatttt catttatttc
    24841 aattaataag tcagtatttc catgtataat tgttaatggt gtttttatat catgtgccag
    24901 tgcagatatc tgttcctttc tattttcttc tagcatccat tgtttttcta aagaattctt
    24961 taacgccaac ttcattttat ccatagaaaa aaggatattg ttaatttcat aaattttact
    25021 agattctaca acaaaatcta aatcctgttg ttctattttt tctgtagtat tttttaaaag
    25081 ctccatttca atattaaatt tcttgccaaa tacttttgat aatataacaa tttcaataaa
    25141 aaatattata ctaaaaataa tcatacctaa catctctggt tttggtaaat atgctcttaa
    25201 aactggagaa ctatactctg atactagttt gtattttatt ataaaaactt catctttctt
    25261 aaaaaacttt atataacttt ctgaatatgc atgcctttct tcaatatctc tcattaaatt
    25321 ccatacttgt tttgattctt ttttattaaa agtgcctgag atcaaattcc catctaaagt
    25381 ataaacacca tactcgtata aatttggaat tatatcttca attgcttttt cacttgaaat
    25441 aatcttttct ttatatttat caaattgttt ttctgaataa ttagctggta atattacacc
    25501 tgatgataat aatacagaaa atgataacat taaaagaagt gcaaatgaaa ttgtcataac
    25561 aaagaataaa gataaatatt ttataaaaat agttcttaac ttttcaactt ttttgttgtt
    25621 agatttttta cttctaagca tttgtgccat atcctccaaa tattttaatt aattcataat
    25681 taatacctaa ccagttttta tttgattatt ttatattttt cattgctaag ttatatttta
    25741 acataataat ataaatataa tgtaaataaa cagtcaattt ttatttattc ttctgacttt
    25801 ttaccttccc atttagaaaa ccaaatacat aaaattacaa atgctaaaat tgttacaaaa
    25861 ccacacatta aacctgttga ttgatatgct tttattgatt caacctttat atattcaacc
    25921 gttttagaac ttgaaaaaga cgaccaaata gttataaatc taacacccca tgcacaagga
    25981 aaatactgcc aaatgccaga acccaatcca gttaataata atgctgaaag taacatttca
    26041 actataccta ctccaattga tgttcctttt ccgaacctta aacttaagaa taaatgtaat
    26101 atgtattcaa ataaattaca cccaattaaa attagaattg aaatcatata aaagcttgat
    26161 gtaaagtaag ccttagatat accaaattta aaacctaata cagacactaa tgttgctaca
    26221 aaaccaagac atactaagat tatatattta cttataagag taagatattt tggattagat
    26281 gttgttaaca tatgtttata attccctgca taatattctt gttcaacaaa cattgaacac
    26341 acaacacctg acaataaagg aaaagctatt gctataactt gtaaatatgc tactatcttt
    26401 aatatattat cagctcctga aaccttataa taccataaaa acaatcccat ccccattaaa
    26461 gcaatgcaaa tgtgcattag tattataaaa tttctcttta attttattaa atcagaacaa
    26521 aatattctta ttagttgtct catattactt cacctctcta ttattgaacc aagctcttgt
    26581 aactaaagca aataatacaa ataaaataac agatattaat actcctggta aaataacagc
    26641 attatttaaa agttcactcc cctctggtac aggaagccca tttggtagaa tatgcaatac
    26701 aacaaccatt aatcttgaag gaatcgaata tggaaataca taccacattt ctgtagttga
    26761 gaaaacagca actccaacaa tagtaaagaa catatttgtt agaactgtaa caaatatacc
    26821 aaattttgaa gctaaaaaca tacaaagagg aatctgccac ataaatagta aaataattaa
    26881 tatagttgca atgatatttt gacctattga aatagtttct ttatagatta tcccaacaaa
    26941 aactacacct attgcagaaa taatcgatgc tatagaacaa caaataatac agtaaatcat
    27001 ttttccagcc caaacgtctt tcatttcaat tggcaatggt aaaataccac gatatttact
    27061 tttttcatct tttaatacta ttaaagaaga tattaatgtt aatgctcctg gaagaaatgt
    27121 cgtataccac cagttgaaag cagcattttg ccctccacct agtccacaca aaagtataca
    27181 aaacactgga actatccata tcaacttcat tccaaaagtt cctttcatct tttgaaattc
    27241 cactctaata aatctttcca tattactccc ctctctttct attttcagct acaactttca
    27301 taaaaagttt ttctaagttt tgttcttttg atacttctcc ttgataacca attactccat
    27361 tagaaattat tccgatttca tctacgactt gttcaacttc agacaagata tggcttgata
    27421 aaataactgt aattccttgt tttggaaaag aacgaatcaa ttttctaagt tcttcaatac
    27481 caatagggtc aagaccattt gtaggctcgt ctaagattaa aagttttggg tgatttaata
    27541 atgcaatagc aataccaaga cgttgcttca tacctagaga gaattgacct gaacgctttt
    27601 taaaagtatc tcttaagtcg acagtctcta aaacttcttc aattcgagag tcaggaagac
    27661 caagtaaagt agtatggact tttaaatttt ctcgtgctgt taaattctca tatatagcag
    27721 gagactcaat aagagaacca atatttgata aatcctttct agtccatcta tgtccctcaa
    27781 acattatttt tcctgaagtt ggatttaaaa tcccagttat catttttaat aaagtagatt
    27841 tcccagctcc atttggtcct aataatccat atatagaatt agatttaatt gctagtgaaa
    27901 tattatcaac tgccatctgc tccttaaatt ttttagaaac tccttttacc tgcaaaatat
    27961 aattattcat aattttcatc ctttcttttg tcaaacttat ttacaagaat cattctaaat
    28021 cataaatata aggattttat aagaattaaa aaacaaaaaa aagatgtgtt aaaaacacat
    28081 ctttttttat cttaattcta ttaattttcc attgtagtat atctctactt cttcaatcca
    28141 aatgctatta tcttcttctt ggatgacatt tttaataata ttatttatag tatatattat
    28201 attttgttca ttcaaataat catctttcca agaaactcta ctttttttat ctggttctat
    28261 caagtttatt ttaacaagat tatctttttc cttaacttct acttccatgt ctaaaccatt
    28321 aaaatactca tttgataatg tatctaagat taatcgcagt ttatccttaa gtgaactttc
    28381 ttcatcaatc tgtaagtact ttactatttt ctcatccttt gaaattatct ctataggtat
    28441 tttagtagtt tttttagaat taatcatttt actagttgtt tcttcttttg catcttcatc
    28501 cttctttaca actgctgatt ctttagaatc aactagctca actgcactga tgctataatc
    28561 catacatgct gtactcaata aagtagtagc aattattaaa aaacttactg ctattttatt
    28621 cataagtatc cctctcctaa ttctctttcg atacttatta taccatagta tagattatat
    28681 ttttatactt ttaaatcaat ttataaatta ttatagtaaa ttatttacaa ttctaagcta
    28741 tttgaattta tttttatagt atattctttc tctaaattcg acagattttt attcaaaagc
    28801 ccttttattt ccctcgtatt tctatacttg ttgctatgtt cttcactatg ttcataatca
    28861 tatgaatcat tgtcatattc ttcatcatta ttaaattctt caattgcttc ttttgacttt
    28921 atatcacaat taaatttata cttatttact ggtatatcaa gtcttacctt agaatttgga
    28981 acttctatgt ccaggaactt agcaattggc atattagagt acaagtaaaa ttcactttca
    29041 taatttgaat ttctacctat tttctctttt atatttacat tttcaggtat atactcttct
    29101 atattattga taaatacatc ttcattctct gaagatatat ccaatgactc tgattctatg
    29161 tttacatttt taacgcctaa aattgatttt gttgacaagt gaagattgct attagatatt
    29221 aagtttaggt tttctaatct atttactttg ttctctgtta tagctctact aaaacctcca
    29281 taagaagttt tatatgttat agtatttgaa gctacattat catatacaaa taagtatgaa
    29341 cttgtacttg atgcatttat gtctacatta tttggtacat atattataat ttctctataa
    29401 tcatgtgaaa acatactatt tatagatgta tttaataatt ggtcgaaatt tttaatcttt
    29461 ttaattttat tttcatattt tctttcacct tttacaacta aagtcttatc ttttaaagtt
    29521 acattgtatt tgtaaaactc attatttcct cttgttgtta cacgcacatc ttcacttgaa
    29581 cttttcttta ttgtaacgtt tatattgtca acagatatat ctaacttgtc aatatttact
    29641 ttttcttgat aaagtttatt ttctttttta aattcagctt ccttttctaa accaaagttt
    29701 attaggcttg gcataactaa aatccctgac catgttgtcc ctattatacc tattaccaat
    29761 aaaactgcag caaatatagc aagtttttta ttcatgttta tcttctcctt tcttattttc
    29821 tatatcttca aggtccttat atatcgcctc aaaatttata ctatcatcat tatattcatc
    29881 agtttctttt atgctttctt cttctcta
//
LOCUS       DAEPOG010000028        29031 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEPOG010000028 DAEPOG010000000
VERSION     DAEPOG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.028"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..202)
                     /locus_tag="KR432_001100"
     CDS             complement(<1..202)
                     /locus_tag="KR432_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639651.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKC"
     gene            complement(397..946)
                     /locus_tag="KR432_001101"
                     /pseudo
     CDS             complement(397..946)
                     /locus_tag="KR432_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(1229..1708)
                     /locus_tag="KR432_001102"
     CDS             complement(1229..1708)
                     /locus_tag="KR432_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBH0639652.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(1701..1838)
                     /locus_tag="KR432_001103"
     CDS             complement(1701..1838)
                     /locus_tag="KR432_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639653.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(1862..3232)
                     /locus_tag="KR432_001104"
     CDS             complement(1862..3232)
                     /locus_tag="KR432_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0639654.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(3181..3507)
                     /locus_tag="KR432_001105"
     CDS             complement(3181..3507)
                     /locus_tag="KR432_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBH0639655.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(3789..6206)
                     /locus_tag="KR432_001106"
     CDS             complement(3789..6206)
                     /locus_tag="KR432_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639656.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(6218..8176)
                     /locus_tag="KR432_001107"
     CDS             complement(6218..8176)
                     /locus_tag="KR432_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBH0639657.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(8176..8748)
                     /locus_tag="KR432_001108"
     CDS             complement(8176..8748)
                     /locus_tag="KR432_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBH0639658.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(8771..9343)
                     /locus_tag="KR432_001109"
     CDS             complement(8771..9343)
                     /locus_tag="KR432_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBH0639659.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(9396..9731)
                     /locus_tag="KR432_001110"
     CDS             complement(9396..9731)
                     /locus_tag="KR432_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639660.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(9746..10912)
                     /locus_tag="KR432_001111"
     CDS             complement(9746..10912)
                     /locus_tag="KR432_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBH0639661.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(10924..11361)
                     /locus_tag="KR432_001112"
     CDS             complement(10924..11361)
                     /locus_tag="KR432_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639662.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(11432..11581)
                     /locus_tag="KR432_001113"
     CDS             complement(11432..11581)
                     /locus_tag="KR432_001113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639663.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(11562..11954)
                     /locus_tag="KR432_001114"
     CDS             complement(11562..11954)
                     /locus_tag="KR432_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639664.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(11985..12182)
                     /locus_tag="KR432_001115"
     CDS             complement(11985..12182)
                     /locus_tag="KR432_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639665.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            12258..12632
                     /locus_tag="KR432_001116"
     CDS             12258..12632
                     /locus_tag="KR432_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639666.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(12642..12851)
                     /locus_tag="KR432_001117"
     CDS             complement(12642..12851)
                     /locus_tag="KR432_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639667.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(12898..13707)
                     /locus_tag="KR432_001118"
     CDS             complement(12898..13707)
                     /locus_tag="KR432_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBH0639668.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(13755..13958)
                     /locus_tag="KR432_001119"
     CDS             complement(13755..13958)
                     /locus_tag="KR432_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639669.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            14135..14476
                     /locus_tag="KR432_001120"
     CDS             14135..14476
                     /locus_tag="KR432_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0639670.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            14488..15036
                     /locus_tag="KR432_001121"
     CDS             14488..15036
                     /locus_tag="KR432_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639671.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            15112..15618
                     /locus_tag="KR432_001122"
     CDS             15112..15618
                     /locus_tag="KR432_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH0639672.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            16238..17107
                     /locus_tag="KR432_001123"
     CDS             16238..17107
                     /locus_tag="KR432_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBH0639673.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            17120..17827
                     /locus_tag="KR432_001124"
     CDS             17120..17827
                     /locus_tag="KR432_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0639674.1"
                     /translation="MSIKNGKDFLYVIWKYPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            17906..19036
                     /locus_tag="KR432_001125"
     CDS             17906..19036
                     /locus_tag="KR432_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0639675.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(19394..21082)
                     /gene="ilvB"
                     /locus_tag="KR432_001126"
     CDS             complement(19394..21082)
                     /gene="ilvB"
                     /locus_tag="KR432_001126"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBH0639676.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(21135..22130)
                     /gene="ilvC"
                     /locus_tag="KR432_001127"
     CDS             complement(21135..22130)
                     /gene="ilvC"
                     /locus_tag="KR432_001127"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH0639677.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(22400..22639)
                     /locus_tag="KR432_001128"
     CDS             complement(22400..22639)
                     /locus_tag="KR432_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0639678.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     misc_binding    complement(22752..23009)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23170..23931)
                     /locus_tag="KR432_001129"
     CDS             complement(23170..23931)
                     /locus_tag="KR432_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH0639679.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(23936..24757)
                     /locus_tag="KR432_001130"
     CDS             complement(23936..24757)
                     /locus_tag="KR432_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBH0639680.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(24799..25554)
                     /locus_tag="KR432_001131"
     CDS             complement(24799..25554)
                     /locus_tag="KR432_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0639681.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            complement(25597..26466)
                     /locus_tag="KR432_001132"
     CDS             complement(25597..26466)
                     /locus_tag="KR432_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0639682.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(26478..26960)
                     /locus_tag="KR432_001133"
     CDS             complement(26478..26960)
                     /locus_tag="KR432_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0639683.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(27012..27944)
                     /locus_tag="KR432_001134"
     CDS             complement(27012..27944)
                     /locus_tag="KR432_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0639684.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(28037..28870)
                     /locus_tag="KR432_001135"
     CDS             complement(28037..28870)
                     /locus_tag="KR432_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0639685.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT         9256 a         5090 c         3050 g        11635 t
ORIGIN      
        1 tacattttac ttctgtaatc caaaactttt tatctttagt aatattaatt ccatttttgt
       61 tttctttaca atattcttta tccttaaaga aggtctttat tacctctttt aaaatttctt
      121 catctgaata gacactccca ttaattttat ttataatatc tattctataa acatcaatat
      181 atcttttttt aaaattactc attataccat tattcctttc acaaatattt ataatgatat
      241 aattctatat aatcagtcaa tatccttcaa taatcattcg acaaccacaa aataagtcta
      301 aataatactt ttaccttatt cctgtctata atgtacaaat atatttcatt tttaattcat
      361 tacataaaaa aaaagaccct ctattaatac agtcatttaa atcatttcga ttaattcttt
      421 aatcttttta tatacctctt tataattcat acctttatct atcaacctag gtaatttcat
      481 agatataact ctttcaagta cttgtattaa taaaaaaact cttgtatttc atctgtaaaa
      541 tcttttctga ccttttacct tctgttctta tatccagcca ttcttgatct ttttctgtgg
      601 caatatagta accatgtatt ctaatttgtt taagtgcttt tgtaacccac ttcgtaaata
      661 gttttgcttc tgctttatta cttctaaatg acatattgta cacagcttct tctgtaacaa
      721 aagtagtacc aaagttaggc aatttatctt taaagtttct agtgtaggaa tctccgacag
      781 tagactcatt aaatttcttt ttatattctc tatctatatt tcttaatgta tcacgaatat
      841 ttactatgcc tagttcctct cctacgtcat ttgcattaaa ccaaacttct tctccatttt
      901 ttgaccatat catccttaca tttttctctt gtaaaatttt caacatacta ctacctccta
      961 aattagttat taataaagaa tattcaattt gaacaccctt attttcgacc cttcaatatg
     1021 agagattaaa attgtataaa aaaagaccta gaaatcactc taagtcatga attactcatt
     1081 tatcattctc cttatttaca aaataaaaaa gactaagttg gggtaactta gcctttttca
     1141 agggggaata tattatacac ttgtttcata ctaccattat aacaggggtt aaatatcatt
     1201 aaaatatcat ctttttatca tgtatttatc aaacagttaa tttcaagcca tctataccaa
     1261 ataaatacac tccaagttct tttaccattt cattaatcca acgtcttgat gtaactactc
     1321 cacaatttaa tatctctgct atttcttcat atgttttttc atcacaatag taaagctcta
     1381 aagctctata cttttcataa gaatgtaaac gctcttgtct taatttcaaa gttttcattg
     1441 ctgaatctat atgtgctatc ataataatag ttcttgcttt acttttcttt atacttaaaa
     1501 tatacaactc ttcattatca agcttaccta aatcatcctc taagtaatct acatctttag
     1561 cttcacttac cgctgagtct atatgtctct taaaatcatt ataatgcttc atcaataatc
     1621 tagtattata taaaacgcct tttttcctat cttctctttc ttcttttctt aactctctta
     1681 caatttcttt tatactttct ttatccactt aaatcactcc ttcttgctct cttcgaacaa
     1741 cttctactgc aatactcaac tttagattta tccaacttat aaaatcttct accacaccaa
     1801 gcacatctta ctattttacc tgggcttata agttccatct tttcttttta ctttcatacc
     1861 ttcactccct atattctttt aattttgctt ttactgcttc aagtaatgca ctttgccctt
     1921 tatccttatt ctctatagct tccattactt gttcatctat tgtaccttta cagattagat
     1981 gatggataat aacagtttcc ctttgaccct gtctataaag ccttgcatta gcttgttgat
     2041 acagttctaa gctccaagtt aatccaaacc aaacaattat acttccacct gattgtaaat
     2101 taagtccatg tcctgtactt gctggatgac aaagtaataa ttgtattctt ccattattcc
     2161 aatcatatat atcttttgag ttttctattg ttcttggttt caaactctta aactctttca
     2221 ttaaacgatt ataatcatgt ttatagttat aaaaaactat aataggctta ccatttgaaa
     2281 catctataat ttcttttaaa gcttctaatt tttctctatg gagttccttt acatttttat
     2341 cattatcata aattgctcca ttagctaatt gtaaaagttt atttgctgct acagctgctg
     2401 atgaagctgt tattatatct ctatctaatt ctaatatctt ctctttttct aattctttgt
     2461 aatatttaag tattttacta tcaaggtgta tttcaatttt attatctatt tttctaggca
     2521 tattcaaata atcttctgct tttaagctta tacaaatatc ttttatttta ttatgtattg
     2581 cattttctgc tccattttta ggttgccaat tatatatagc tcctgtttga taattttttt
     2641 gtcctggctc aaagtatcgt tctttatatc ctgtaatagt tttacctagt ctttcacctc
     2701 tatcaagtaa gtacatctgt gcccataagt ctattaaact atttggtgtt ggtgtaccag
     2761 taagcccaac tactctttta gttaaaggca atactttctt taaactttta aatcgttgtg
     2821 ctttatttga tttaaaagaa ctaagttcat ctatcacaac catatcaaat ggccattttc
     2881 ttttataaaa atccactatc cagggtacca tttctctatt tattatataa atatctgagt
     2941 ctgtacttaa agctctcact ctatctaatt tactaccaag aactttagat acttttagat
     3001 gctttagatg ttcccatttt tttacttcac tactccaggt atctcttgca actcttaaag
     3061 gtgctataac taaaactttt gatatatcaa agtaatcata cattaattca cttatagctg
     3121 ttagagtaca gactgtttta cctaatccca tatctaatag taaacctata ttattattat
     3181 ctatagtttt tctaattgtg tattcttggt atggatgtgg tttaaattcc atcccttaac
     3241 ctccttgata aaatcatcta tctcttttaa tgtgcttaca cacttaactt taaatcctaa
     3301 ttctcttaat tctcgcattt tatattgttg aagcttcctt agttttttac ctggtgcttt
     3361 aagttctaca aatataacat gtccttctgg taataagaca atcctatctg gcacacctgc
     3421 ctcacctggt gaaataaact tcatagcctt tccacctaac aactcaatct cttttttaag
     3481 tcttttttct atttttgatt ctaacaaaat atcacttcct taaaatttaa gtgtcaacaa
     3541 tgtcaacaga ttttctatat atatgtatat atacatatta ggcatacata tgtatatacg
     3601 tatatgccta atattactta tactacttta tacttaattt ttgttgacat tgttgacatt
     3661 aatacctcaa acattgtaat tactgcatct taggtgtcaa cataagcgtc aacaaagcca
     3721 ttttttttgt tgacattgtt gacactttta aaattttcaa atttactctg tttttaattc
     3781 tgttgacact aacttttacg tacataagct ctctgcctac catatatttt cccaaacctt
     3841 aaatttccat tatatgatat ccaatcatct aatcctttta atatatcatt aatttctctt
     3901 gataagatag gcgtgagttg tttaggttct ccattaaata gctcaaccca tatctccata
     3961 acacacgttt tttctcttaa tattgttcct tcttttaaat caccaaaatc tgaaccatga
     4021 atatattctc tcttttctga aatacttaaa tcataccaat tctcagtaat aggtttattt
     4081 agatactctt cgataattcc agatttagca ttttcttctg agtgagttct ttgttgtctt
     4141 tcagcttctt ttttctcctc atctgataga tatagctgtt cattggcttt acataattct
     4201 actgcttctg cccaaatttg atttctttca ttatcaagtt gaccattaaa tatactcttg
     4261 ttaaccttct ttactcctgt atctattggc caaaaccttc tattcccggt cttatctctt
     4321 aaaaattctt tatcattagt tgttcctata actacacatt gacgtaaaaa ccttgatgtt
     4381 ctcttcccat atgcgaccct atatatatcc tctgatttgc ttagaaaatg cttaactgcc
     4441 tcaatatctg cttttttagt agccatcatt tcacccattt ccagcaacca tactccttgt
     4501 aattgttcat acgcttcttt accttgtaca gtagttaaac tatcagaata ccagtctcca
     4561 ccaagttttt taataaaagt actttttccc attccttgag gaccagataa aaccatcata
     4621 ttatcaaact ttattcctgg attaaatact cttgctactg ctgctactaa aacttttctt
     4681 attatagtcc ttgtatagtg gttatcttct gcacctaaat agtcaattaa aagtgtatcc
     4741 actctcttaa tgccatccca ctttaaagaa tttaaataat cttttatagg atgaaaagta
     4801 ttattttcaa aagcaattat tagagcatca tttacttttg atggtgatga gatattgtaa
     4861 attgtttcta tatgatgtct aagcccagaa tcatcactat cattccaatc atttaattta
     4921 tcatcttttc tccaaggtaa cttacctaaa acgacagctc tatttgaaaa ttcattataa
     4981 gctatttttc ctttcaaata tgggtcattt tctataaaca ttaagatatt gtttgttgtt
     5041 ttcttataac ttcccttatt gtcataatct aacctagtta accattcatc atcttcaaaa
     5101 tctatatcac caaaatcatc cttagcttta tcaaggtttt ctcttcctat agtctttcgt
     5161 actttagtgt cactgcttgc aaattcgctc attcgagtaa atgaaggtaa tctatttaca
     5221 ggcgtttcgg gtttagcatc ttcatcaagt tcaccaaatt tatgtatcct aactaaatca
     5281 aaagcattgc ataaaattcc gcttgctggg tctgtaccat gatgactata tgaaaacttg
     5341 tcatcataaa taactactcc accacttgta ctaccttctg aatatgtata cctggtttca
     5401 tcaataccag gaatatatac ttcatttaag aaattttcta tagtttcttt tatactatag
     5461 gacctgcaaa atgcacctat aattccagcc ttttcaatag ggtcttgttg tttttttaat
     5521 tgtgtattaa acttttgcct ctccctcgaa ctttctggcc aatagcttac atctgtccag
     5581 tctaaatata aatctagtat ttcatttggg tttaaaaact ctccatcttg aaatttaaat
     5641 atatagtctc catcaattga agtacttgga aaatacataa gtctatgagg ttgatatgta
     5701 gtatcatcaa acatatctat tcctatagta tctgctatca tcctggaaat tgcttgatat
     5761 tcttctggta gtactggtct agttaaaggt attacaagtc tatatctggg attattctca
     5821 gtatgtgagt gcgtagaata cataagacaa gcataatcat ttaataatgt tatatcctcc
     5881 catatatctt tattcgcata atctatatct aaagttataa tacttctatt tgcaatattc
     5941 tctgcttttc gtcttccatt ttttaagctt ccacctacaa atccaccaac atctttaact
     6001 ctatcttttt cagtctttga catcttccta tattctgtat atgtttcttg agttcttaat
     6061 gttttactta atctatttac aagttcggac cataaaatac ttttattttt ccaatgtgtt
     6121 tctaatttat tttttcctat ggccagcatg agttggccat catgtcttac atttatgtgt
     6181 tcaatttcac tggcctttat atccataaat caatcaccta gtctttctta taataatcac
     6241 attcatatcc atctgcttta agaggaagac ctttagccca agatatttct tttcccataa
     6301 tactgttaac ttcttctaaa gaacctcctt ttttatctac atcaattaca agctcatcat
     6361 gtacatgcat tacaatgcta taacctgcat ctgtcacatt aaacatagcc tctcttaagc
     6421 aatctctagc tgtagcttga acaatattct caactaactt aggtccataa gtatctattc
     6481 ttttccattg tttacttgtc tgttccatac cttcatatgt tatcttatcc ccactaaatg
     6541 tagtatgagg ctctatctta ggtcttagat atgataatct tctaccactt ggtagttcta
     6601 taaataaaac tcctggatta taaataaatt taatcccatg ttggagtttt actatagtcc
     6661 tatcttttat ggctttttta gctgccttat ctacatccca ccaaaattta gttatatttg
     6721 gattagcatt tctccatgtt gttacaagag gttgaagctc ctcttcatca agacccattt
     6781 ttatagcccc cattgatgtt aaagctccta tgcttccccc atatccaaga gctagctctg
     6841 aaatcttacc tttttgtcta agtgggtcac cttttttaat actttctatt ggaactttaa
     6901 acatatgact agcactagct tcatatattt tcccgtgaga attaaataca tctaatctcc
     6961 acttctcacc agcgaaccaa gctataaccc tagcttctat tgcactaaaa tctgatacta
     7021 taaatctatg accttcactt ggtataaaag ctgtccttat caattgactt aagacatctg
     7081 gtacactatc atataaaagc tctattaaat caaaatctcc ttcttttaat aggtttctag
     7141 ctaggtctaa atcctctata tgattttgtg gtaaattctg tacttgtact aatcttcctg
     7201 cccatctacc agtcctatta gccccataaa actgtagtag acctcttact ctattgtcat
     7261 tacctttagc taatttcata gcctcatatt tctttataga agttttggac attaattttc
     7321 taagttctaa aattctaact acattttcat catcaacttg ttttaatatt tctggaatac
     7381 tttcttttgt taggcttgta atctcaaagc caactttatc acttaaccat ttttttaatt
     7441 gagctggact atttggatta tttagaccag ttattttaat tgcttctttt gtaagttttt
     7501 cagtatatct tttatcacat tctattgcat tctctattaa ctctgtatca actttaatac
     7561 cagtatcatt aattctctgg tctaaatacc ataatttaat ttctctttcg gtagtcttat
     7621 acttactaag tttgtttctt atttctcttt caactacaac atcttgttta caatattctt
     7681 taaatttatt ccatttttcc atatcatgta ttggtaaatt tctagttctt cccttgttaa
     7741 cttttgtagc cttacaaggt ttacagaaat attgtattaa tgctttacct tctttcattt
     7801 tctgtttatc ttcattaaac tttaaagcct tagacacact atctaaactt cctggaagtc
     7861 ctagtgttaa cgcctttatc attgtacatg accactcatt tggttttaaa ttaatattta
     7921 aaaatttact tattgctgtt ctttcaaaat tagcattaaa tgctgatttt ataactttat
     7981 tatcatttaa agcttctatt acttcttttg gtaactcttc atcatttact aaatcaataa
     8041 cttttacctc ttcattatca aaagcatagg caaataacag tatctcaaaa ttagcagagt
     8101 ctacatatct gtagactcca acttttttta tatctaaatc actatatgtt tctatatcaa
     8161 ttgataaggt cctcattaac ttaagaagtc ctcttcttca tcttcatatt caaagtcatc
     8221 tgagaaatct gcttctgcac tagctctagc tcctccaagt acttctccat ctgctaactt
     8281 ttgaacattt tgtaagccac aacctattcc tttatttcct gcactattat atgggaaaaa
     8341 gtttatacta actcttccat aacaaccact atatacctct gtattatcta atatttcatt
     8401 taagtcttta tcaactattc ctggcttttg tgtactattt gcatttaaaa aatacattcc
     8461 tacatattcc tctgcttcat cagctctttc tgcatctcca tcacgaagag gtgtttttaa
     8521 attacctggt aattttccac cccatttaga agttttacct tgttctttag ctgcatcaat
     8581 agccttcttt attcttccta aagtcacctt atctgatttt ggtattaaaa tacaaactga
     8641 atacttgggc tctgcacctt ctaccattgc tctgctttta aagatattgc aataacttaa
     8701 tcttaccttt cctgttacta cctttgttga ttgtactgaa ttactcataa ttttcttcct
     8761 cctaatattt ttatatttgg tatattgatt ttatatttga agttatttct ccaaatttat
     8821 ctaaatctct aacttgctta ctaaacaatt taattcttcc acaaataact gttgaagaaa
     8881 catttaataa ctctcctata tcagatatct ttttatcttg taaaatccaa tctactattt
     8941 tatcaatatc ttttaaatat gactttcttg caatttttct tatataatca acatcatagt
     9001 taaattgaat ttcataaaat ccatgtatat cttttaatgg tcttttacta tcttcaaata
     9061 cacttacatc taaaccttct atagagtttc tataatgttt ttcgccataa cgcaaagctt
     9121 taaacatttc tattcttatg taagaaacag ccactgtaga aaacttgcct ctgttactat
     9181 cataatttaa tgctgcttta tataacccaa tacatccttc ttgataaaac tcttcatata
     9241 agtatggatg ttgctctaca taagacttaa aaaatctatt tatactaaaa tgtacaagat
     9301 tcaaattatt ttcaactagt ttagttattt cttgattact cataaaatta tatccccttt
     9361 taaatctttt tatagctact aggaattaaa tatttttaat tatcctttgc tcttactgat
     9421 acaaagcata ctggactaac ttttatatgt tctggtatat acactctatc aagatgattg
     9481 tcatttactt cgaggaaagt tgacatcact ttatccttta ttaaactttc tcttttaaaa
     9541 tccatcacta taacttttgc attttcttca gttgagttat attctgtaat atttagtctc
     9601 tttaacatat ctagtaaatt atctttttca taatctaaat ctttttcgat actctttttt
     9661 ctatccttta aatctaaaat tatacttatg ctttcatcta atttatcttt tatacctgca
     9721 tctaatggca tgtaatatcc cccatttata tttcaaaatc ttttttcgct gaatctatac
     9781 tattaatttc tgacctttta tcactttcta ctactaaagt agcttttcca actggtttta
     9841 taattaaatc acttagtagt ttagcaaaac tctttttacc tatagctttc tccatatcac
     9901 taattccttt taaggtcctt ggcttataaa ttttttcttc atcataatct gaatttaata
     9961 aaaccttagc aacttcttgc tcatctacat actttctatt acttctgcct tctacaagct
    10021 tatatcctgg atatttaaca ccatgctttt cagcttgttc tagtgcataa ctttgaacat
    10081 cctttaacca atcttgtata ttcttagcaa aacctaaaat atctgctatc tcatatttat
    10141 taagagcaaa tgtgtcagca aaatcatatt ttctagctag tttaaggtta tcctcagctc
    10201 tttttctgca atcattttta gctctacaaa atccacaatg actgccactt acaaactctc
    10261 cttcaccatt aaaagccatt tgagccttct tcttaacatt atctgcccat gtaagtaatt
    10321 tagtaacttc tatttcttca cttgatatgt tatcaagtct aggttgaatt attgttgttt
    10381 taattaaatc aatatcgtat agcatttcaa actgattata tgcccctaaa ccatatagtc
    10441 taagttgagg attctctata gctgaaactt ctaaaccttt tccatacttt aggtctataa
    10501 cttgaagtat accatcagat attacaacaa cgtctcctgt tccaaatcct tctggaaccc
    10561 actcactaaa atcgagtctt tcttctaaca tcactattac atcatcacat atagctttac
    10621 tatcatttac taattcaact acattctcaa cataagattg tatatagtcc tccatttcag
    10681 agttataata ctcacttttc tgtatctttt taattcttgc attatatgcc tttttactta
    10741 ttttttcata ctctagcatt aatttaactt ctgctaattc atgtgcaact gttccttctt
    10801 ctgcatatat actagttaat ggtggatagt tttcttctaa ttttatacta ggagtgcagt
    10861 gaagccatct atgggctcca ctcgcactaa gtcttgcatg ttgtaatggc atttatgtac
    10921 cccctataaa ctttctaatt tgttcataaa agctgaataa tcttcttctt ttacttcact
    10981 taacttacta gctccaaatt caccaaataa ctccttaagc ttatccttct tacctgcctt
    11041 acttacttgt gctgccttag ttcttacctc ttcttttgtg tatttaactt cactagttgt
    11101 attttcatta ttcttttcta tttcttcctt tgcttctgct atttcttctt taacgtcttc
    11161 ttttacttct acctttttaa tcttttcagc ttttttaact tcaactttct tctcttcttt
    11221 catatctgtt atatttatag gttctatctg cattgctttt cctagattaa gacctcctaa
    11281 agcattcgcc actactaata gtgcatttgt aaattctggt gcttctactt taacttttac
    11341 atttacatta acttcaacca ttttaaatat ctcctttttg tgttatactt tacttgtgtt
    11401 atattttatt ttttattttt ttgtgtgttg gttattttac caacactttt ttatttaata
    11461 ttccaaccga tattttctta ccagttttaa tatctttaaa tactatatct gctataactt
    11521 ttccatcttt tttaagagtt actacattct tattattagt atcaagactc agcaatttca
    11581 tccccccact ctactgcttt taaaagttca tccaaatttt taccttgatt tctttcaata
    11641 aaatcatcaa cttcatatct tgaaattttt ctaccatcac ctctaactag tgattttatc
    11701 aaacctgtgc ttactaacct acgcataaaa gctgtatcta actttaaaat tccccttgct
    11761 tcttctactg ttattagata atttggataa cctcttttta ttaaaacaac tatatcttta
    11821 ggctccagta cctttacctt ttgttcaata gtttgactct taactctatc tgtctcttgt
    11881 ttacttatct ctatttctat taaacttttt aaactatcac taaattgttt tgctatactc
    11941 tcagaaatat ccatgtgaaa attctctccc ctctttttaa ttatttatct tccaactaat
    12001 tcgtctaatg taatatctaa ataatcagct aattttatta gagtatctat agttggatta
    12061 gaacttttat ttctcagtaa tttatataaa ccacttacat ctatattcaa ttcttttgcc
    12121 aatttataag gttttatatc tctttttctt agcatttgct ttatattgtc gcctattaac
    12181 attgtattca cctcagttct acatggtata atataaacat ggaataaatt ccaaaatttt
    12241 caaaaggatg gtgtattatg cagttttcaa acaatatatc taaagaatta caagaaagta
    12301 taagaaagac agtctctgat acttctaaca cagagtattt ttactatgct gaatttcaat
    12361 ataaaattat tcttaaatct atagaggaat ttgaaaaaga acttgatgat gaacatgaaa
    12421 ttgcactaaa attgactaac tttggaaaag atgtcttaat gattgttgaa gaagtaggtt
    12481 atcataaccc ttgtctaatt cactactacg gcatagtaaa tggagtttat tctgaaattc
    12541 ttcaacacac atcacagata aactttatga taacttcagt aaagaaaact gacccttcaa
    12601 aacctgctag aagaataggt tttatattgt aattatcatt cttattttct gattctttct
    12661 ttagtaattt cactaatttt tcttttttca cttttattca tctcatgtaa atacaaaact
    12721 atacattggt acatataatc tataaaatca ctatttgatt gagctgttat ctgactaaat
    12781 aactgtgctt gcaattgatt ttcaagcatg gttattcttt tctctatttc ttctattttc
    12841 ttattttcca attcaatctc tcctttctaa aatattctgt atttagtttt caaagtacta
    12901 acctaatatt gaagtttgat aactatatcc tttttctata tctggtaata tgttatttgt
    12961 tttcaataaa tcatataaaa acaatcttcc cttttgagtc cacttagttg tcatttttac
    13021 atcaggcatt ccattacttc tagttatgtc tattgtttct gaatgagtgt atcccttccc
    13081 ttgatgttgt ttatataaaa gccattgtcc actttgttta tattgaatcc ctctttcatg
    13141 aagtatttta ttcatttctt ttccactcat tccatagtct tttgctattt gagttattgt
    13201 gacaagtcct ttatttttta atatcatatc tgtataatct gcctttggtt ttagttcttt
    13261 tattacttgg tctttcattt tactttctag ttgtaactgc tcatttactt ctacttgctc
    13321 tataagatgt tgtaacgctt ctttatatgt agttggtagt ttagtttgca aatctatcat
    13381 attatatgtc ccagtactac gtatacttgg taatatctca ttagttaccc aacgtttaaa
    13441 taattttgca cttggcaact tagaacttaa tattaaagaa taaagaccac tttcattaat
    13501 tactttcata ttttgattac caccaggagt cggtatttca cctactcctt tatcttcatc
    13561 atcaacatgt cttttcaaag catcagatgt atctttatat cctaattgtt ctgcaatatc
    13621 ttttccaaca aaccaaaatt ctccatttag ttcgattact cttatttctc caaaagtctt
    13681 atttttgaat atttgtaaat tattcataat ttctttcctc cttatgattg tttttgtgaa
    13741 caataaattt taatttatgt taaccttttt tctaaaaaaa gttcatccat tgttttattt
    13801 aaaatatagc taatcattat catttctttt tccgtaaaat ttctttttcc attttctttt
    13861 agattatatg ttgattttgc tattcctatc ttatatgcta gttcttcttg ggtatatcct
    13921 gcttctattc gaccagcttt taaacttctt ttcttcatgt tcttcacctt ccaatgtttt
    13981 atttttgtga acttcatact tttattatat tcacacaaac aaaacttgtc aatacataag
    14041 tttatttttt tgttaacttt tttatttttt tctaaacata agtttacgtt tctgtaaact
    14101 tatagtatca tttaattatt gggaggtgtt gtttgtggca acttttggag aacgtttcaa
    14161 acaattacga gaagagaaaa aacttactca agatgaacta gtttcaaaat tcaataaagt
    14221 ttactttaca agttttaata aatcaacaat atcgcaatat gaaaataata aaagaaaacc
    14281 agaaataaat attctagaaa attgggcaga tttttttgat gtatcaattg attatctttt
    14341 gggtagaact cttgttagaa atcacatcga tactgtagcc gcacacagag ttaatcctca
    14401 caaagattta ccagaggaag ctcaagaaca actcaacgat tatattgaat ttttaatgaa
    14461 taaatataaa aaataaggat tgaatctatg ttaatattaa aaaaattgag aactgaaaaa
    14521 ggaatttctt tagataaatt aagcacagac ttaaatataa ataaatccac tttatccaga
    14581 attgaaaatg ggttaagaga accaaaagaa tcatttatta aagattgctc agactatttt
    14641 ggagtatcta cagattactt aattggaaaa attaacatag atgattcaaa taaattaaca
    14701 aaatgcttaa attcaacttt tccaataagg ttaaaagaat taagaaagaa aaaagaatta
    14761 actcaagcag aattatcaaa attacttaat tgttcactaa gcaaaattgc tatgttagaa
    14821 actagcaaaa gagaacctgt taaagaagac ttgttaagaa tttcagaatt ttttaatgtg
    14881 tcagttgatt atctacttgg taaaacctca attgaaaatt acataactac tgatgaaata
    14941 tccaaaataa ttaaatctta tgaaagttta cctaaagaag cgcaagaaca tattaatagc
    15001 tacatagagt ttttagttga tagatataaa aaatgaatat ttagagcagt tcactcctgc
    15061 tctttatata taaaaaagta acacatacat tctttttata gggggatttc aatgaacaaa
    15121 ctagacgcac ttttagactt agcaaataat gaagagatag aaatttacta cactgacaaa
    15181 atagcagatg acataaaagg attgtatata aacagacaag gactaaaaat tatatcatta
    15241 cttaattcat taaaacaaaa caatgctaaa ctaatagaaa tcttagcaga agaattagga
    15301 catcatttta ccagtgttgg aaactatgta tcttcaaaaa acagttacaa aaataaaatc
    15361 ttgatagaca aaactgaaaa caaagcacta aaatgggcat gtgaatttct tataacagaa
    15421 gaagaaataa tacatgttat taattcacac gctacaagtg tatacgaaat agctgaagaa
    15481 ttacaagtta gcatcaactt cttactaaaa agattagaat ttctatcaaa aaagaaaagc
    15541 atgttggact taggaaataa tagattttta gtattaacta atttgccaaa tttctacata
    15601 tatgaggata ttttttaaac tcatttattc tacttttata gattttttac ttaataaata
    15661 tatatttcaa tattattata ataaactaca cataaaagct aaaaaattgt aagaatatta
    15721 agaaaataat taagtaaaaa ccagataaac aaaattaaga taatattgta cataacaaaa
    15781 gtatataaag agcagttaat ctgctctttt atataaacac caaacataca tacattctaa
    15841 aagggagaga tactattatg aaaggtggag taagaaaaag aagcaacaaa tggtattact
    15901 actttgacct aggcatagta tctgaataaa gcaatatcag cttacctttt ggtacaaaaa
    15961 atatttctta cttaacaaca ctcaaaaaaa ttaccaaata aatatagaaa actaaaatta
    16021 agtatttata aagtaaaacc attatacaca gtagtaattt tgatgagtaa acacatattc
    16081 catatgtaat aaagcttgaa gttaaaagtg gaataaataa aatctagaaa aaatttttat
    16141 tttaagttaa gcttattatg gataataaaa ttcttgcaaa tttagaatca aatgatacaa
    16201 taagaatata aaatacttgt ttgagaggat tattttcgtg attaaaaatt ttgatgaatg
    16261 gaaagagtat gatggttttt cttatggtag tggaagaagc gagaaaatct ggctaattaa
    16321 tgaaaaaact aaagatatag gcttgtttaa attccctaaa acaaaagaaa ctggagaata
    16381 ttgggcagaa aaattagcct gtgaaatagc tagtttgtta aagattcctt gtgctaatgt
    16441 tgagataggt acatataata aaagaattgg ttcaatgagt tatttaatca ataaaaatga
    16501 tgagaaattg attgaaggcg tgtggttaat aaacaaatat tatccttgtt ataatacaga
    16561 taagttatat gattataaaa ataatgaata ctattcattg aaaatgattt ttaaatctat
    16621 agaagaatat aacataaaaa atgattttct aaaaatgtta atttttgatt acttgattgg
    16681 aaatagagat aggcatcaaa ataattgggc tctactagaa agtcaaaata aaattactct
    16741 tagtcctcta tatgataacg gatcatcatt atgttgttat gttaatattg agaacataga
    16801 ttctttcttc actgatcatc aaagatttga agctcttata aatacacgtt caagatcaag
    16861 aataaggata gatggaaaaa ataaaaaaga accaacacac ttagaagtgc taaaatattt
    16921 aaattcaaat tattatgaac aaactataga ttttgtagaa aagataataa atcttatgaa
    16981 tgaagtaaac ataaatacat taatagataa ctattcagaa aatattataa gcccaaagtt
    17041 aaaaaaattg ctaaaaatat atctaaaaga aaaagtgtta agaataaaaa atatatataa
    17101 tttgtgaaag agggataaaa tgtcaattaa aaatggaaaa gattttttat atgttatttg
    17161 gaaatatcca aaaagtagaa gacaatttat aattggaaag ttagctaaaa atggattgta
    17221 tgaattttct tatgaatatg aaattgatga agctattaaa gcaggtttta atgctttaat
    17281 atcatttgat gatattagta aaacatatta tagtgaagat ttatttagcc catttgcaag
    17341 tagattacct gataaaaaaa gaaaggacat caataaaatt ttggataagt atgatatgtc
    17401 tgagtatgat acatatgaac ttttaaaaag aagtggtgca agattaccta tagatacact
    17461 agaatttgta gatccaattc ctttggacat tgataaaaat ataattagat atttttatat
    17521 tgcaggagta agacattatg ctgggtgtga aggacatgaa tgtgataatt caatagaatt
    17581 agaatcaaat gaagctctag ttctagagtt agaaccagaa aataaatttg atcctcatgc
    17641 aatcaaggta tttgataaaa aagagaatca tataggttac attcctagat attatagtgg
    17701 agaattaaca agtataataa atagaggatt aaagtataat tgcattatta aatttgttaa
    17761 caaagaaaat aattgtgatg aatgtataaa agttaaatta gaaatattat ttgacgaata
    17821 taattaatgt cttcttgtaa atataggaga cattttttat ataaacacca aacaaacata
    17881 cattctaaaa gggagggata ctattatgaa aggtggagta agaaaaagaa gcaacaaatg
    17941 gtattactac tttgacctag gcatagtaga aggaaaaaga aaaaaagtag aaagagttgg
    18001 tggcaataca aaaaaagaag cagaaaaagc actaagagaa gcactaaatg aatatgaaaa
    18061 ctctggcata gtctttgaag aaagtaacat cagcttatca gactacctag acttttggta
    18121 caaagaatat gttttactca actgtaaata caacactcaa gaaagctacc gaataaacat
    18181 agaaaaacat ataaagccaa aactaggagc ttacaaagta aaagctttaa ctcctgcaat
    18241 actacaaaac ttcataaata aaaagtacaa agaggattat tctcaaaata cattacaagt
    18301 attaaaagcc atattacata ggtcattaaa atcagcagtc catccttaca aacacatacg
    18361 agaaaaccct atgcaatatg taagcatacc aaaaactaaa tctaaaacag aaactaataa
    18421 agttaaaact attacattag aagaatttaa tcaaatacta aatatatttc ctcaagattc
    18481 atttcaacgt atagttttac taattggatt tcatactggt atgcgaagag gtgaaattat
    18541 tgcactaaaa tgggataata tagaccttga taataaaact atcacagtaa agcatacttt
    18601 gattaaaaaa ccaaatggaa tgtttgaatt agggcaacca aaaacagaaa gctcttgcag
    18661 aactatattt acaggtgaca ctttaataaa ggcattaaaa gaacataaat tatatcaaaa
    18721 gaaaatgaaa ttaaaatacg gagaatttta ctttgatagt gattgggtat gtaccaaaga
    18781 aaatggtcaa caagtgaata ctcacacttt agacactata gtaagacaaa ttcgagtagc
    18841 tttaaacaat gacttccatt ttcattcttt aagacatgca catgctactc tattattaga
    18901 aaatggtgct aacattaaag acatacaaaa ccgtttgggt catagccaat tatcaactac
    18961 aatggatacc tattcacatg taactgataa aatgaaaaat gaaactgtag atatatttga
    19021 aaaaattaca aattagagtt tgccacccaa aaatataata cggtggcaaa tgggtggcaa
    19081 aatctaattt atctatttta aaagctaaaa ttatcaaatt tatatagtca gctatacgct
    19141 tgtaatttca aggctttaga gtatataaca accataacta atataaggta ttaataataa
    19201 atctaacgaa taaaacttaa tatttactta agttgtaata ctattctaaa ttgtataatt
    19261 aaaatttaat aagttcttaa ttatatattc attgatttat ttgcaaaaat aaaaaatgcc
    19321 aatctatctc taaacaaaaa ctgaatacca ataaaaattt aataaaaaat tttatcaata
    19381 ttcagtataa gttttagtta catatcaaat tttccaatgc atcattaggt gctacaatag
    19441 gataaacatc ataatcctta tctatatcac attgtaaaaa tacaggttta ttaaaatcta
    19501 tcacatctag aacctttcct aattcttcca tattggatac cttatatcca tcaatattat
    19561 atgcatttac gagtttaaca taatccacat tttcattaat atcagtttga gagtatcttt
    19621 gattggaaaa taacttctgc cattgtctta ccattcctaa tgttctatta ttgagtagta
    19681 aaataagcat aggtacatta taatttgcaa ctgtagctag ttcattacag ttcattctaa
    19741 aacttccatc accagttact aaaaccacat ttttatctac attaccagct ttagttccaa
    19801 tagctgcacc taaaccaaat cccatagttc ctaaccctgc tgatgttata aaacttttat
    19861 tacctttaaa attccagtat ttagcagacc acatttgatg ttgaccgaca tcagttacta
    19921 ccacagtggg tttctttaat gtttcatatt tttcatttat ttttttaagt atattttgtg
    19981 gatgaaattc atatgtttgt acaccctcac tctttctaaa tgtctttatc tcttctttcc
    20041 aattgctatt attcttactt tcaacccttt caattaataa actaaggact agctttacat
    20101 ctccaaccaa agatacatta gattctatat ttttacttat ttcagatggg tctatgtcta
    20161 tgtgtattat ttttgcattt ttagcaaatt cagaactttt acttatcact ctatcactaa
    20221 atctcgcacc tatcgctatt actaaatctg aattagaaag tgctaaatta ctttctctac
    20281 ttccatgcat tccaaccatt ccaagagata gttcattctt tctatctata tttccaagcc
    20341 ccataagtgt atttagtacg ggtgtatcta tcttagttgc aaatttttca agaatttcct
    20401 cactgtctga agatttaaca cctccacctg catatattac aggcttttta gattctttta
    20461 tgatatctat tgcttcattt aaaagcttaa tattagtttc atcatctaga tcaaaatcac
    20521 tcttataatc catataatca tctatttgac ataaatcata gtcctcacca ctaaaatcca
    20581 tttctgctaa aaataaatct tttggaacat ctacaagtac aggtcctttt ctacctgaat
    20641 ttgcaactct aaaagcttct tttattgtag gaactaattc tttagtgttt ctaactaaat
    20701 aattatgttt tgtcatagat aatgttgctc cagttatgtc aatttcttga aaagaatctt
    20761 ttcctaataa actagttggc acctgccctg aaattacaac cataggtgaa gaatccataa
    20821 aagctgttgc tattccagtt atggcatttg ttgctcctgg accagatgtt gtaaaacaaa
    20881 ctcctactgt attagtactt cttgcgtacc catctgcagc atgaactaaa ccttgttcat
    20941 gggatgtcct tatatgttta aaatcatctg aataatcgta tagagcatca taaagaggta
    21001 tcacggctcc accaggatat ccaaatattg tgtcaattcc ctctttcttt aaacattcta
    21061 aaattacctt tgctccattc atacgcacta ctatacacca ctctttccct caaactaatc
    21121 catttaatat atatttattt taagaatgac atcatacttc ttagatttct accaacttcg
    21181 gcaatttctg tattatattc ttcttctcta gtttttagga agttttcacg tccttcttca
    21241 ttttctttaa tccacgcttt tgcaaatttt ccattttgta tatcctctaa aacattttgc
    21301 ataccttgtt ttgcagcatc agttattacc cttttaccag atacataatc tccatattct
    21361 gctgtgtcac ttatactata tctcattctt tcaaatccac cttcatatat aaggtcgact
    21421 attagtttca tttcatgtaa acattcaaag tatgctactt ctttttgata acctgcatct
    21481 acaagcgttt tatatcccaa ttttataagt tcacttattc caccacaaag tactgattgt
    21541 tctccaaata aatctgtttc tgtttcttct ttaaaagtag tttctaaaac acctgctctt
    21601 gttgctccta tcccttttgc atatgctaaa actgtctctg tagctttttt agtgtagtct
    21661 tgatatactg caaatagtgc aggaactcca cttccctttg taaatacatt tctaactaag
    21721 tgaccaggtc ctttaggagc taccattaca acatcaacaa attctggtgg ttgtacttga
    21781 ttataatgta tattgaatcc atgagcaaaa gctaatgtct gaccttcttt taagttatcc
    21841 tttacactct cttcataaac tttcttttgt ttttcatcag gcattaacat cattgtcaaa
    21901 tcactttctt ttgtagcttc tgctacactc ttaacttcaa atccatcttc ttttgctttt
    21961 tgtgctgatt tacttccgtc ataaagacct atcactacat gtacaccgtt atctctaaga
    22021 ttttgtgcat gtgcatgtcc ttgacttcca taacctaaaa ctgcaacctt tttatttttt
    22081 aaaacctcta aatccacatc tttttcataa tacattcttg ccattttcat ttcctcccaa
    22141 tactttttct tatttttatg atatttattt ttattagaaa ttcatactca ttatcattgt
    22201 tttaatatta ttcaacagat ttaaattcta aatttatata atcttacaat tataatttta
    22261 gaattttcag tttttattag ctacaacaaa ctaataaatt tattaactgt gataaactga
    22321 taaattcaat gacacttata ttttaatcta agatttttct tatttaatat ttatctttag
    22381 ctttataaaa tatatttaat taatgtctca ttctttcaac agttatctct cttatatcat
    22441 gtagtttttt catatggttt aagacagaat ctaaagatgt cttttcttca tttactatta
    22501 attttatacc tgaattattc atattatcta cagtcatact tattgattgt atatcaacct
    22561 ctcttcttct tagagtcata gccactctta aaaaagaatc tattccttgg tttaatttag
    22621 catatacagt attttccata aaaatccctc cttattttta ttattttttt attaatttta
    22681 tatggattaa atttatttat atcgcttaaa ataaaaaaac tttcatcttt ataagcaata
    22741 taaatattac ttataaggac gaaagttata ttccgcggta ccaccttatt tgtagatata
    22801 aaaaatacat ctacccctct tttttcaggt cgggtaatta attaccttaa ccttagtcat
    22861 gtaacgttga catacgtttg tacttactaa aaatattcag tacaaaagct ccagagtgat
    22921 cattatacta ctgttaacac caattctcag caacattggt tctctgtgga taacactatt
    22981 agtattgttg tctctgtcat tgcttttaat tttttaattt tatattttaa ttctgttttg
    23041 ttgttaatat agattatatt ccctatcatt tacaatgtca atgacttttt ttaaaatttt
    23101 cagattattt atttttttca caataatact tcttttatac tttacgcctg tattttggaa
    23161 taatcgtttt cagattatta ttttatatcc atttattttt agccaatttt ctttatcttt
    23221 ataatcgggc ataatggatt ttactaaatc atagaatttt ttagagtgat tcatatgtac
    23281 taaatggcta aactcatgta ttattacata atcaattaca tctaatcttg ccatagatat
    23341 ttttgagttt atataaatat ttttttgagc tgtacaactt ccccatctct tattttgttc
    23401 ttttacctta acacttgctg gaactaattg cttcattatt tcacaattta atttaagaaa
    23461 atctattctc tctaatactg ttctttcact ttccatctta taccaagatt ttaaagattt
    23521 tttaataaaa tctgtacttg tatcatttgt ctgaacaact atttgatatt cgttaatagt
    23581 tattctacag ttactttttt tgacagaatc tttcaacagt tcttttataa ctagatggta
    23641 ctcttcacct aaataaagaa atttttcgcc agttataaac attctttgtt tgtatacttc
    23701 ttctcttcct ttatattttt ctgacttttt aagtatccag tctccttttt caataattat
    23761 atcttttatt atttttttgg atattttagg aggactcatt acaattattt tgccaaatgg
    23821 atctattttt atagaaatat tttttctatt tttatatact aaaactgctt ttatttcaat
    23881 ccctctaaaa ttaacattta aagtatcaat atctttactc cctgtgttca ttagtttatt
    23941 tttccttatt agctctacaa tttataagtt cagcagctat acatacagct atttcagctg
    24001 gtgtttgagc attcaatttt atgcctattg gtgcatgtac ttttgataat tcttccttct
    24061 taaaaccttt tttcatgagt ttttcgtacg tcaatccaat ttttgttcta cttccaatca
    24121 tacctatata accagtttct cttttaagag cccattctaa agcacttgaa tcatatagat
    24181 gacctcttgt taaaataatt atatagctat tttcgtcagt tggaaaatct ggtatatttt
    24241 caaaagaatc taaaactact acttctgatt ctgggaacct ttcataatta gcaaattctt
    24301 ctctatcatc tattactact gttctaaatt ctaaaagaga tagtatcaca gcaacatctc
    24361 ttgaaatatg acctgcacca aatatatatg ctttacttgt attttccttt agtctcttat
    24421 tgaatttttc tatattactt tttaatttac tatctataaa atctatttgt atttctccag
    24481 tacctccaca tatcattcct agttttgcca catcagattt gtttagagta aatttataca
    24541 acattgattc tctatttttt ataacattaa ttgcatgttt tatacattct gcttctacac
    24601 tacctcctcc tactgaacca aatatagtag aatcattttt cacaatcatc attgctccag
    24661 tttcacgtgg tgttgaacct gaatgagacg ttattgtagc aaatgcgaaa tcttcattat
    24721 tttctattaa tcttattgct tgttcatata tattcatata attctcctta ccagtctatc
    24781 taaaaatctt ctatatatct atcttagtct ttttattact ttatttggta tcacaaattc
    24841 tactgctccc atataaccag gtactacttc atattcttca aaactaatca caagatttcc
    24901 atttttatta aaataaaaat cttgatactt atttatagat tcaaaatctt tcactgggaa
    24961 gtctttatct tggtctataa aataagattt tgtatcatct ttcttaattt gatttctcat
    25021 ctgctctttt atattatcac ttatagctct aatatatcta ctatctttaa aaaatattgg
    25081 taaatttaat agtattcctc tatttttatc aacagtgtaa tgcttcttag ttatagacga
    25141 ggaatcttct atctcttctt ttgtcatttc tattgataaa aagtccttag aattattttt
    25201 gacattatgg attacattta ggtatctatg acccttatta ttttcctttt tcattttatt
    25261 ttgaaattca ctgtaaattt ttttcccatc ttgcaaaaac tcattgttta atttattctc
    25321 taaagtttta tcttttaaac cttttatttt aggaatactc acattcactt cataattgcc
    25381 ttgtttaaag ttgagactct tagcatttaa cacctttatc gtatcactat ttgaagtctc
    25441 attagaggaa ctacctgcaa atatagggtt tatatctact aatgagacta ataagcatat
    25501 tgaaaatata gaaattttaa atatagattt tttaagcttt ttatatgtag tcatattgtc
    25561 cttcttaaaa tatgtatata taaactacta ttcaatttac aaatctttta ttactttata
    25621 tggtattaca aactcaactg cacccatata accaggtgct acttcatatt catcaaaaca
    25681 aattacaagg tcttcatttt tattaaaata aaaatcctga tacttattaa ttgttttaaa
    25741 atcttcaaca ggcaagtctt ttttttgatc tataaaatag gattttgtac tatccttttt
    25801 catttgttct ctcatttgtt cttttatatt atcacttata acttttatat acttatcatc
    25861 tttaaaaagc attggcaaag tcaaaactat ttgtcttttt ttatcaatag tataatgtgc
    25921 cttactcaca tatgatgaag cctctatctc attttttgtc atctctattg atagaaattt
    25981 tttagaatta ttttttacac tataactcaa acttaaagat ttatatcctt tattctttga
    26041 tgattgtatc ttctccattc tctcttggaa ttgatgataa agcctttttc catcttccat
    26101 aaaactactg tttaatcttt gttctaagtt tttatctttt aaacctcgta ttttaggaat
    26161 atcaatctca gcttcataat tttcactttt taaactataa ttttttatag ttattacttc
    26221 tactattact ccaattacag gtatcttaga aaaattgtct gcaaatacag gacttatatt
    26281 tactaatact gtaaatatac atgcaaaggc tattgcaact ttaaatctta atctatttac
    26341 ttttttattt ttctctcgat atatgttttc tattttttca tgatttatta tagtatctaa
    26401 tttcttgggt attggaattt tatgatactc atcttttaac ttatttaacc tttctttgct
    26461 tgtcattctt tttatcccta ctcttcaatt tttaatttta gtttttttaa agcagtatat
    26521 aacctagttt taacagtatt cacattttca tccaatatta ttgctacttc ttcaatcttt
    26581 aaatcttcaa aaaatcttaa tattataata gatttgtatt caataggtaa ttcttccaaa
    26641 gctcttctta aatccaaatc agtatattta tcatactctc cagtctcatc tataaggtca
    26701 atatctgtca tattattata tttacggttt tttctaataa aatctatact cgttctaact
    26761 aaaattttat aaaaccatgt ctttatataa tttacttctt taatattttc cactgagtat
    26821 agtgccttat acatggcttc ttgaaccaca tctaaggcat cttcttcatt cttcgtataa
    26881 ctatatgcta ttctataaaa agagtcttta ttatcgataa tgtatttctc aactttcgtt
    26941 ctctttaaac tcttaaacat tcctgtaaaa tctccctccg attacaggct aggattgagc
    27001 tgttagaaat tctatctgtc tattttagca tcttcattca gtttttttat atgctcaata
    27061 tacttttcat ttaaaataga ttctcttatt aaactctgac actcattaaa acttttttgt
    27121 ctttctcttt tatcagtaac tttgactata tgatatccat aactagtttc aaacacattt
    27181 gaaacttcat ttttctttaa tttgaacact tcctttgtaa actcagcatt tttatcatcc
    27241 tttgtaaaat atcctagctg tcctccattt tttccagttg ctttatcatc cgaatatttt
    27301 ttagctaaac tttcaaaact ttctccattt ttgattttag tcaatatatt atctgccttc
    27361 ttcttcaaag cttctttttt atctttgctt acttcttttt tatttttatc taaagtcgat
    27421 ataagtatct gagaagcact tacttcttct acattaaaat cttttttatg acttgtatag
    27481 taggcttcca tatcattatc acttatgttt attctatctt caaaggcctt tttattttta
    27541 tctattgcta aatctctaga tatttcttgt tttaaatatt ctttatcaac acctgctttg
    27601 tccatttttt ccttatagct tgtattcaaa ttcatcttat cttctagttc ttgatatttt
    27661 gagtctactt cacttttact tggagttagt cctttatcct tagcctcttg atataaaagt
    27721 tcattatcca ccataaacga tattataaca ttttctaatt tatttctacc ttttttatcc
    27781 aaagtttttt ctaattcatc taaagactgc ccatttatat aattattagt agcagataaa
    27841 actgcttttg tttttttgta ctgttctttt gtgatttcca catcatttac ttttgctaaa
    27901 cttttattac aaccaaccat catcaaagac aatattccga ccattcctat ataaagtttt
    27961 ttattcaata tacctacacc tcactcattt ttattctcaa tttctaaaaa tatcaatcaa
    28021 tattcttata ttttaactac tctaaattac gtaaccaata attttttggt atttggtttt
    28081 gatctactgg cgcaaattca taaccttcat ctgataagaa ttttaaaact gaatctaaag
    28141 cttctgcact ctgttttttc tcatgcatta aaactacaag ttggtcttta tcttcacctt
    28201 ttctattctt aatatgattt tttacatttt gtacaatttg agaagaatta gatctccaat
    28261 cttctgtatc taagtcccag tcccaaattt tatatccagc atcaactaaa gcctgataag
    28321 aggcttgtgg tgtatatggt ttgcttccat aaggtattct tattactttt gaatgtttac
    28381 cagttatctt ataaaaagtt tgatcatttg tatcaaattc ctcttttgct gatgtactag
    28441 taacataaag cttgtgaata tcatgtgata cactatgaaa acctgctgtg ttcccatttt
    28501 taattatatt tttaacttgc tgtggatatt ccttcatatt cccatctatc ataaaaaaag
    28561 ttgctttaac attgtattta tttaaagttt ttatcatctc gtcagtaaat ttagatgggc
    28621 catcatctat agttatgtat gctatttttc cagaactttt acttggccct ttactaactt
    28681 ctattgatgt attagatact atatctgtaa tagtagaact tgacagtttt gcatttctac
    28741 taatttcatt ttccttcata tttaaagagc tgacgcgaac accaactact ataaaagcca
    28801 gacacactac aatagcactt aaaagcaaat acaacttctt tctatttatt tttttctttt
    28861 tatttctcat cgttcgacct ccaatattac tatattttta catttatatt tttgtagtat
    28921 tttatcccaa aaatttacac tacatatata tagacgttga acttcgacaa aaagttttgc
    28981 ttaattttta aaatttgtta tttcggtgat tattatgctg tataatttct g
//
LOCUS       DAEPOG010000029        28744 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEPOG010000029 DAEPOG010000000
VERSION     DAEPOG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.029"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(94..549)
                     /locus_tag="KR432_001136"
     CDS             complement(94..549)
                     /locus_tag="KR432_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639686.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(777..1196)
                     /locus_tag="KR432_001137"
     CDS             complement(777..1196)
                     /locus_tag="KR432_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639687.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            1495..1704
                     /locus_tag="KR432_001138"
     CDS             1495..1704
                     /locus_tag="KR432_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639688.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1736..1945
                     /locus_tag="KR432_001139"
     CDS             1736..1945
                     /locus_tag="KR432_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639689.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(2162..2560)
                     /locus_tag="KR432_001140"
     CDS             complement(2162..2560)
                     /locus_tag="KR432_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0639690.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(2981..5477)
                     /locus_tag="KR432_001141"
                     /pseudo
     CDS             complement(2981..5477)
                     /locus_tag="KR432_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5491..6033)
                     /locus_tag="KR432_001142"
     CDS             complement(5491..6033)
                     /locus_tag="KR432_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0639691.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            6276..7790
                     /gene="glpK"
                     /locus_tag="KR432_001143"
     CDS             6276..7790
                     /gene="glpK"
                     /locus_tag="KR432_001143"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0639692.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(7904..8533)
                     /locus_tag="KR432_001144"
     CDS             complement(7904..8533)
                     /locus_tag="KR432_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639693.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            9122..9913
                     /locus_tag="KR432_001145"
     CDS             9122..9913
                     /locus_tag="KR432_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBH0639694.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            9942..11162
                     /locus_tag="KR432_001146"
     CDS             9942..11162
                     /locus_tag="KR432_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBH0639695.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            11182..11877
                     /gene="pxpB"
                     /locus_tag="KR432_001147"
     CDS             11182..11877
                     /gene="pxpB"
                     /locus_tag="KR432_001147"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH0639696.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            11907..12893
                     /locus_tag="KR432_001148"
     CDS             11907..12893
                     /locus_tag="KR432_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBH0639697.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(13112..13678)
                     /locus_tag="KR432_001149"
     CDS             complement(13112..13678)
                     /locus_tag="KR432_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639698.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            14030..15097
                     /locus_tag="KR432_001150"
     CDS             14030..15097
                     /locus_tag="KR432_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBH0639699.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            15116..16276
                     /locus_tag="KR432_001151"
     CDS             15116..16276
                     /locus_tag="KR432_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0639700.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            16289..18385
                     /locus_tag="KR432_001152"
     CDS             16289..18385
                     /locus_tag="KR432_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBH0639701.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(18544..18888)
                     /locus_tag="KR432_001153"
     CDS             complement(18544..18888)
                     /locus_tag="KR432_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0639702.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            19180..20070
                     /locus_tag="KR432_001154"
     CDS             19180..20070
                     /locus_tag="KR432_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0639703.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            20288..21574
                     /locus_tag="KR432_001155"
     CDS             20288..21574
                     /locus_tag="KR432_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0639704.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(21663..22106)
                     /locus_tag="KR432_001156"
     CDS             complement(21663..22106)
                     /locus_tag="KR432_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBH0639705.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(22130..22924)
                     /locus_tag="KR432_001157"
     CDS             complement(22130..22924)
                     /locus_tag="KR432_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBH0639706.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(23490..23966)
                     /locus_tag="KR432_001158"
     CDS             complement(23490..23966)
                     /locus_tag="KR432_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0639707.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(24067..25236)
                     /locus_tag="KR432_001159"
     CDS             complement(24067..25236)
                     /locus_tag="KR432_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0639708.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            25399..26262
                     /locus_tag="KR432_001160"
     CDS             25399..26262
                     /locus_tag="KR432_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBH0639709.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            26333..26926
                     /locus_tag="KR432_001161"
     CDS             26333..26926
                     /locus_tag="KR432_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBH0639710.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(27177..27812)
                     /locus_tag="KR432_001162"
     CDS             complement(27177..27812)
                     /locus_tag="KR432_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0639711.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(27892..28623)
                     /locus_tag="KR432_001163"
     CDS             complement(27892..28623)
                     /locus_tag="KR432_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0639712.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT        10699 a         3475 c         4561 g        10009 t
ORIGIN      
        1 atataaaaat aacatataaa aattcaaaaa ggagttaagc ttaaatttga ttagaaaaaa
       61 tcaattttaa gacaactcct tttttttatt aaattattgt ctattaacca aaatagctat
      121 tttagcatct ggattataac ttatctgaac catttgattt ttcttaacat gttcaaggtc
      181 ttcaccacca taagctattt gtaacttaac tggtaactta ccttgtttta taatagcaac
      241 gtactctttt ttaccttttt tctctaaact aatcaaattg ccaacataag gtttaaagtt
      301 ctgatacttt ttactagaat ttcttatgta gaagaaagca ccaacagcaa taactaaatt
      361 tatgccaagt gtaacccaag aattgatttt aagcatagct ccagcgatta ttatcacgaa
      421 cattaaaacg ataggtaata tagctttctt aagaagcaat ttacccatta tttcattagc
      481 ctttttctca gggccactca tagtttttga tctagcaaat gattgcgcga atttgtctct
      541 taagcccatt ttatcctcct aattttaata aatatttagt tataataacg agatattact
      601 tgaaactaaa aatttactac atttatatta tgtttgactt ttgtataaat aattacattc
      661 aagtaaagca aaatatacta attattttat cataaaatta taaaaaagaa aataaatgaa
      721 ataaaaatat tagaacaaag aaatgatgta aaatcgtatc aaaagcaaca taaaaattat
      781 ttatctattt tctcatcttt atttttgtta tactcaattt ttcctaaatc cttctctttt
      841 tcatattcat gaagttttaa ttcaatcata ccttctattt tggctttatc ataatcattt
      901 aactttctaa agttgtttaa aagctttatt tcattagagt ttatgctatt aagtggatag
      961 tttgaggagg aatcgcaaat taaatctgat ttatgtgata gattatctcc atttaatagc
     1021 cagtctactg aaacattaaa tatctcagct atagatttta atatttcata atttggtttt
     1081 ctaatgtttc tctcaaattt acttaagttg tcacagccta acatttcttc tagttcatat
     1141 tgtttaaggt tttttgcttt tctcaaataa acaattcttt ctcctaaagt atccataaac
     1201 actctccatt caattaatgt caaaaagact ttttaagatg taaatagttt caaattaaag
     1261 gtcaaaatga cataaaaacc attgacttaa ggtcaaaatg actttataat taacttaatg
     1321 atacgaattt acatcctaat tttagcacaa agtaatcaaa aaatcttatt tagtattaaa
     1381 taaatttata tacttaatat gtgtacatat taaaaatata tactaaatag agggggtgcg
     1441 taagctaaag taatataaaa gtaaatataa atcacttaga aaggaagttg ataaatggat
     1501 gctcgaaaaa aatggatacc ttttttggga gtgcaagtca agcaaagact tattgaatta
     1561 aatatgactc aaagggaatt agcgaagaaa ataggtgtta atgaaaacta tttgtcagct
     1621 attttaaatg gaagaagaac aggtaaaaaa tataaatcat caatttatca attacttaat
     1681 atagaatatt cagaagatga ttaataaata gtatataaag taggtgaata ttcttgtgtg
     1741 caaattggat tcagatgggg ttatagagtg ttgtagagca attgatgatt ttattacagc
     1801 acttagtaat ataaaaagct taaatatgga aagattaaat actttaacta aatattctag
     1861 tacatgttca atccttctta aagaggggaa ttatgaagga tgtacaattg tgtatagaaa
     1921 gatgttggaa gaattaaaaa catgagtaat gcatttctta ggaatataaa ttatacatag
     1981 aaatgtatta tatttttcaa agtacttaaa ctaaaatatg gataagataa tctaaatatt
     2041 ataaatgtgc ttgaaattag actatacttg tttttaaata atccaatatc catattttag
     2101 taatatacta caaaaaaaga aggttaatag atgatgtaaa atcgtatcaa attatgtatg
     2161 tttaaaccat tttatcttca ttattattag aggaatgctt ttttaagtct ttatattcag
     2221 atatcttaag ttcaagtatt ccttctattt ttattttatc acgttcgttt agttgtctgt
     2281 atagatttaa tatcatcatt tcatcattag taacatgtaa gtaatcttct ttatcttctt
     2341 ttacactact attgacattt accttctctt taccatagag aagccagtca gtcgtaacat
     2401 taaaataatc agctattgac attagtatat cacaattagg ttttctatct cctgtttcat
     2461 acttgcctaa gttttcaaat tttaaaatat ccataagttt gcgctgagta agttttttgg
     2521 agtttctcaa ataagcaatt ctttttccta aagtatccac aaaatacact cctttctttt
     2581 tatgagtaat gtctaaatga catttgaaat taaaaatata taaatttata atataaaact
     2641 actaaattaa agtctaaatg acattttgct taaattaata tgctcataat atgattttaa
     2701 catattatag ttgaaaatat atggtttatt ttgatttgta tatataacaa tagatttaat
     2761 tgttataaaa atgtaaaggg gtgtatgaat agattgtata aatttatttc gataaactaa
     2821 gattgctttt tgattgtctg taaaagagaa aaagattaag ataaaaatag tattatattg
     2881 taatttatat taatcaatta caaagatttt atgaatttat tctttagggt aaaatattta
     2941 agaataagat aaatttacaa tataatacta taacactctt ttatctagtt ttattttctt
     3001 tatagaacaa taatattata aatgctagta gatttacaca gaatactgtt atatacatct
     3061 gtttgaatcc tgagtttaga gtagattgta gtgtattttc tatttttgtt ctattatttt
     3121 ctatggaatt tatataatcc aattttgatt tatcaaaagc ctttggtatt tcagttttta
     3181 cctcttgtgt ctttgctaaa actgcttgga gtgatttttt ttgctgttgc attgtatcaa
     3241 ggcttttttc catgtctttc ttttggttta tagaattatt taacttgctt tgtagctctt
     3301 tcttttgaga ttccattgat cttagttttt tacttaattc gttcttttga gttttatttt
     3361 tttgtaactt tgtatttaag tcatctcttg tcttttttag ttgctgtatt tgagcattta
     3421 aactttcttt ttgttcattt acatttggtt tattctcgct ggaattgtta ttcttatttt
     3481 ctgtagaatc attcttagtg tcgtctgatt cagaattact ggtattactc tggttttgtt
     3541 catttgagtt attttcgtta tcagtaggag tcttttctgt agtaggtatt ttattataag
     3601 cagttgtaag ttccttgatg gcctcatctt gtttttcaag accttgttcc atacctttta
     3661 tacctttgtc aacaccagca ataccttttt ttacaccatc tattccttga ttaataccag
     3721 ctctagcttt taccatattg tcaataccag tttgaactcc tttaatacca ctatttagtt
     3781 tattttcacc atcttcaata ccgtttattc cactttgtgt tccatctatt cctttttgta
     3841 catttttctg tattttagta attatagatg gaactttttc atcaaacatt gttgttgata
     3901 tttcttttgt tatattggta atgttggttg tatctgctga ctgaacttta ttaagtaagg
     3961 attttggaag tttgccacct gtcatgtcca tatttatatt tgaagtatca ttcatatttg
     4021 ggatagagac attttttaaa ttttttgcca tttcagggtc tgattttaag tcatctatca
     4081 tgttttcaag ttcttcaagt tgaactattt taggagttga gggctttcca actaagtcca
     4141 taatattatc ttgcacagat aatcctgcat gtgcttgcta taaatccaat cattatagct
     4201 ggagaaattg aagtaccaat tgagcgtatc aaggataatg ttgcaagtgc tgaattagat
     4261 tcttctaatc ttgtatttga taacatcata tagtttagag gagttcccat agtaaagccc
     4321 attcctaatc ccataaatat taaacttaca catacactaa atacagtatt ggtttttaga
     4381 gcaattaaaa ctaggtaaag agagcctatc atagatacag aaaatcctag taatagtatt
     4441 ttctttgccc caaacttgtc aataagtctt ccgcttaaag gagcagcaat acctgcaaat
     4501 aatcccaata tagctacaaa atatccacca gaaccagcat ttatctttaa tgcattttca
     4561 gcatactgtg gaacgaatac catacccatc attccaatac caacaattaa agataaaata
     4621 agtactatca atattctcgg atttaaaaaa tattcaaagc ttaaaatagg gtctttagct
     4681 ctattttcaa taaatatgaa aataggtata agtacaataa atgtcaataa gtatggataa
     4741 actgatatat cttgtataga atttttaaaa ttaaaaaagt ctatattcat aagaccatat
     4801 aatagtgata cgattataca tatcaacatc aatgtaccaa gcttatctat tttttcttta
     4861 gattcagact tattgttttt aatacagaaa actccaccaa taagtatgat taaacaaatt
     4921 ggaaggttta caaaaaatag ccacttccaa ttttgagttc caaagatact tagaattgtg
     4981 ctaccaatgc ttgaacctaa aatattggca ataccatatg ttgccccaac taaacctaaa
     5041 gccatgcctc ttttattttc tgggaaggta gttccaaatt ctgctgtagc aattggcatg
     5101 attccaccac caccaattgc ttgaataaca cgaccaatta ataaaatata gaaattggaa
     5161 aatagtgatg ataatccaca tattaatgaa ccactaccaa atagaaatat actaactaga
     5221 tacacatatt ttcttcctat tttatctgct aattttccag agataggaat tacagaagcg
     5281 tatgttaatg tgtatatggt aatcatccaa attcccgttt tttcatttac tccaaggcta
     5341 ttttgtataa tcgttcttgc tggtgatact attccagtat caatagctcc cataaatata
     5401 cctaatagat aaagtgtaag tactaaaaat atttttggat tactgtcctt gttcttaata
     5461 ctagtattat gattcaaggt aaacaccacc ttaatgtatt aatttttcaa tatagggttt
     5521 taaatcttca aattctatat gaagatatgt tgaatatgaa aaaaacattt ttattatgat
     5581 attgcatact aatttgtcat tattaaaaaa actttctttt tctaaagctt catatacttt
     5641 atttgaaagc tgttcatgaa agtcgtcttt gtgttttttc atttcaaaaa atctctctgt
     5701 ttcaaaagtg tttaaaaagt cattgtattg gatattatga actttgttta taaaatttct
     5761 tatttcagta aaaataatgt ttagtttttt tatgtaatct tcagtgtttt tattggagta
     5821 ttcaattcgc cttatcaata aatcccattc agattttgtg atatattcta ttatttcttg
     5881 ttttgaatta aagtaattat atactgtacc tgcacttata ccacaccttt gagctatttt
     5941 ccttatgcta atattcgagt aatcttcttc taaaatcata tcccgagata cttttaatat
     6001 atcttctttt acattttcta aaatctttgg catattacca cctcttttat taaaattatt
     6061 atgaacactg tttattatac tcctttaaaa tttattaatc aatatgtaag ctataatgta
     6121 taatattatt atatagatat gatagaaaat aagtgtcttt ttataaatat ctattatatt
     6181 aaatttgtac ttaagtatga ttatttattg aaatatgtga tattaaattt ttctttttaa
     6241 taaattaaat attatatgat aaggagaata tggtgatgga aaagaagtat gtaatggcat
     6301 tagaccaagg aacaaccagt tcaagagcta tactatttaa taaaaaaggc gaaatagtga
     6361 aaattgcaca aaaagagttc aatcaaattt atccaaaagc tggatgggta gagcatgacc
     6421 ctatggaaat atgggggtca caaagtggtg taatgagaga agttatagaa acagcaggta
     6481 taagaccaga agaaattgct tcaataggga taactaatca aagagagact acggttgttt
     6541 ggagtaggta tactggtaaa cctatttata acgcaatagt atggcagtgt aggagaacat
     6601 cagaaatatg tgatgaactt aaaaataaag gattggaaga atcaataaaa gaaaaaacag
     6661 gtcttttaat agatgcatac ttttcagcaa caaaagtaaa atggatatta gataatgtag
     6721 aaggagctag ggaaaaagca gaacaaggag agttattatt tggaactata gatacatggt
     6781 taatatggaa tttaactcgt gggaaagtac atgtaacaga ttactcaaat gcatctagaa
     6841 caatgatgta caatataaat gatttagaat gggatgagga tatattacga gaattggaca
     6901 taccaataag catgttgcca ttagtaaaac cttctagcta tgtttatggt catacagatg
     6961 agcgcatgtt atcaggagct aaaataccta ttgcaggatg tgctggagac caacaagcag
     7021 cattatttgg tcaaaattgt gttgaagaag gtactgcaaa aaatacttat ggaacgggtt
     7081 gtttcttact tatgaataca ggcagtaaca tagttaaatc taagcatggg ttacttacaa
     7141 caattgcttg gggagttgat ggaaaagtaa catatgcatt agaaggaagt atatttattg
     7201 gaggagcatc tgttcaatgg cttagagatg aattaaagat aattgaatct gcaaaagata
     7261 gtgaaatgta tgcaaatagg gtagaggata ctaatggtgt atatgtagtg ccagcattta
     7321 caggtcttgg agctccatat tgggatatgt atgctagggg ttcaatttta ggacttacaa
     7381 gaggagcaaa aaaagaacat atagtaagag ccacattgga atcaattgct tatcagacaa
     7441 aggatgtttt agaagcaatg caaaacgatt caaaattaaa actaaaatca ttaaaagttg
     7501 atggtggagc aagtaataat aattttttaa tgcaatttca atcagatata ttaaatgtag
     7561 atatagatag acccaaaata gtagaaacta cagctttagg agctgcttat ttagcagggt
     7621 tgtctgttgg attctatatt ggaagaaatg aaattacttc aaagtggtca gtagaaaaag
     7681 agtttaatcc aaatatgagt gaagagaaga gatgtaaatt atataaagga tggaaaaaag
     7741 ctgtatcgag agctttaagc tgggaaaaag aagatgaatt ggatatttaa aaattaatca
     7801 aagttaaatt tatttgaatt taaaaagagc tttatgtaaa atatgaagct ctttttttgt
     7861 tgttaatttt gcatttttat ataatcatac taaattatat aatttatttt tcgttttcat
     7921 ataaaaacat tggtatagaa ttgtaaatgt gttggtcacc aactactaag agaatgtctc
     7981 cttctaaaaa ttcaatatat ggtccaggag atattataag ttcttcacct cttttaattg
     8041 caactattgt agcacctgta ttttgccaaa atttagtttg tgcagctgtt tgaccaatat
     8101 atttgcaatt agaatttatg gtgaactgaa cgggcactaa aggattgatt atttcaagtc
     8161 tgttggaata gtctaaaatt ttatttatag tatctaatat ttcattttct aaattagatt
     8221 tagtatctat taaattataa agtgttgatt tatatgatga tatatttgta atatctctat
     8281 atttttttat aaaagaagaa gctttgtctg gagatagtac aataacacct ttacccttta
     8341 tgcttttaac aacgccaatg tcctctaaaa ttttgacagc tcttcttatc gtttctggag
     8401 atacattgta tttaccagcc aaaacagagc gtccaaatag tactgaatcc tcttgtattt
     8461 cacccgtgta tattttattt gcaatatcca acgctatttt ttggtatatt ggcatcgtat
     8521 agtttttatc cattgttttt cccccaatac aaaatttatg ttaaaaaaat atataatctt
     8581 aacaatacta ttgtataatt tataggttta tatgtctaca atatgtacaa atattgtgca
     8641 tgttttaaaa taatatttta acatgcattt agtatacatg attttattat acttaaaact
     8701 atataaagaa ttaattataa aaatataaag atattttact tacaaagaat gataattata
     8761 tagatgaaag aaacaaataa atgaagaaaa atgattctgt cttgcatata aagtgattaa
     8821 aaatgaaaaa tactttaaaa atgaaggtta aggcccttat tttaagtgaa aataattaat
     8881 tattaactaa aaaatggtaa aaagattttt tgaaaaagaa atacaaatga atgttcaatt
     8941 aaaataattg caaaaatatt ccttaaatgg taaaattata ttaattaaat gcaatagaat
     9001 agttgcaaaa ataaaattga atacaagtga taaaatattt catgcaatat ataaaataaa
     9061 caaaatttaa aaattaaaac tacttattct atcaaaatgt tagcaaggag ggggtcataa
     9121 attgtataaa gtagatttaa atagtgattt aggagaaagt tttggaacgt ataagattgg
     9181 tttagatgaa gaagttttaa aatatatttc atcagctaat atagcttgtg ggtttcatgc
     9241 aggtgaccct agtcatatgg aaaaaacagt acagctagct aaaaaaaatg gagtgaaaat
     9301 aggcgcacat ccaggtttcc tagatttaat aggatttgga agacgtgaga tgaaaataac
     9361 aaagcaggaa gctaaagatt atacaaaata tcaattgggt gctttaatgg catttgcaag
     9421 ttcaaatgga tgtaatatac aacatgtaaa accacatgga gcattataca atatggcagc
     9481 taaagataaa gagttagcta tggggatttg tgaagctata tatgaagtcg ataaagatat
     9541 tatactgttg gggctatata atagtgaaat gataaattct gctaaagaaa ttggacttag
     9601 gtttgcaaat gaagtatttg cagatagagc ttatgataat aatggttttt tggttccaag
     9661 aagtgtagaa ggagcagtta tacatgatac aaaacatgca atagataggg ttgttaggat
     9721 ggtaaaagaa ggaactgtag aaactttaac aggtgaagtt atacatatca aagcagattc
     9781 tatatgtgta catggggaca atccaaaagc aatagagttt gtcaaagaaa taagaaagcg
     9841 ttttgaatta gaaggtatag aagtttgtcc attagaaaac atagaagttt gttcgtcaga
     9901 gaacatagta taagactgat aacatgaggg ggagataaaa tatgagtaca aaaaatatta
     9961 cagataaaac taaaaataaa aaagatgtcg gagcacttat aggggctgca tttataatgg
    10021 caacatcagc aattggacca gggtttttaa ctcagactgc acaatttacc caagattttg
    10081 gaccaaattt ttcatttgta attttaatta caacaatcct ttttataggg gctcaagtaa
    10141 atgtatggag agtaattggt gtatcaggac ttagaggtca ggatatagca aataaaataa
    10201 taccgggatt aggttattta gtagcttttt tagtagctct tggaggatta gcttttaata
    10261 taggaaatgt tggtggagct gcacttggta tgaatgtaat gtttaatatg aatatgacat
    10321 taggaactgt attaagtgga ttaatagcta tatttgtatt tatgtcaaaa aattcaaatt
    10381 ctctagtcga taagataact aaatttttag cattaggaat gataattata gttggatatg
    10441 tagcaatatc aaatcatcct cctgttggag aggcagtatc aagaatggta aaaccagaaa
    10501 atccaaaagg attgatattc ccaataataa ctctacttgg aggaagtgtt ggaggttata
    10561 taacttttgc tggaggacat agattaatag acggtggaat aacaggagaa gaaaatataa
    10621 aagaaataac taaatcttct cttttgggta tattagttga cacaatgatg agagttcttt
    10681 tatttttagc aatactggca gtggtttcaa aaggtcttca acttgaccca gaaaatccag
    10741 ctgcttcagc ttttaaattt agtgcaggag caataggata taaattcttt ggacttgtat
    10801 tatggtctgc tgcaattaca tcagttatag gagctgctta tacatcagtt tctttcttaa
    10861 agacactaaa tccttttata gataaatatg aaaaatattt tataatagca tttatagcaa
    10921 tatcaacact tataatggca tttataggaa aaccagctac tttattaatt ttagcaggag
    10981 ctttaaatgg attgatactt ccaatcacat taggaattat gcttatagca tctaaaagaa
    11041 aagatattgt tggagattac aagcatccta catggctttt aatatttgga ttaatagtag
    11101 ttttaatatc tgcatataca ggaataactt ctcttagcag cctaggtgct ttatttgctt
    11161 aatttagaag aggtgacatt tatggaaact agatacttga tatcagggga taaggcagtg
    11221 gttgctgaat ttggaaatga gatatcagaa gatataaaca aaaaagtaat ttcatttatg
    11281 agagcaatag aaatatcgaa tcttaaaggt attgtaacag aaatggtacc aacatatagg
    11341 tcacttatga taagttataa tccattagaa atagattttg atagtttgat tgagaattta
    11401 aaaaaaatag aagataattt ggaaagtata gtattaccaa aacctaaaat acatgagatt
    11461 ccagtatgtt atgatgaggt ttttggtata gatattaaaa acgttgcaag ctacaataat
    11521 ctcactgtag atgaagttat aaaaatacac acaagcagag agtacttgat atatatgcta
    11581 gggtttactc ctggatttcc atatctaggt ggtatggatg aaagaatagc tacacctaga
    11641 ttagaagtgc caaggactaa aatttatggt gggagtgttg gaatagcagg ttcccagact
    11701 ggtgtatatc ctattgatag tcctggtgga tggcaaataa ttggtaggac acctttaaaa
    11761 ttatatgatg aaaatagaga agaacagatt ttgcttagag ctggagactt tataaaattt
    11821 gttccaataa cattagatga atttatagaa atcgaaaaaa ataacttaag tatttaaata
    11881 tcgttatagt acagggagtg gtatatatgg gattcaaagt tgaattagga ggtttccaaa
    11941 cattaataca agacagaggt agagttggct atggtcaata tggtgtatct ggatgtggag
    12001 caatggatga gtatgcacat agagtaggaa atatattagt aggaaattct gaagatgagg
    12061 cttctttaga agttttaatg ttaggtccta caattacctt tgatgaatat acgcaaatag
    12121 cagtaactgg gggagattta ggggctaaaa taaatggaaa agaaattcaa aactggaggt
    12181 catatcaaat aaatccaggt gatgtattat catttagagg tgtgaaatca ggtgctagag
    12241 cctatgtgtc aatagctggt ggaatagatg ttccacttgc aatgggaagt aagtcaactt
    12301 atacaagatc taaaattggc ggatttgaag gtagagcact taaaaaagga gattatataa
    12361 atacttttat tcaggaaaaa gattttacaa taaataaaaa attaaattca aaatatattc
    12421 caacttatag ttcagaaatc gtactaagaa ttgtaaaagg tcctcaattt gatgcttttt
    12481 ctaacggtga agtagagaaa tttttatcaa ataaatacaa agttacgaat gaaatagata
    12541 gaatgggttg tagactagat ggagagagta taaagcacct taatggagca gatattatat
    12601 ctgatggtat ttcttatgga gctatacaag tacctggtca tggaaaaccg attataatgt
    12661 tatcagatag acagactagt ggtggctata ctaagatagg gaacgttata agtgtagatt
    12721 tgtataagtt agcacaagca aagccaaatg atgtagtaaa atttgaactt gtagatatat
    12781 atgaagcaca tagactattg agagagcaag aagataagat tcaagatatc tacaagagta
    12841 tgaaaaatat aagagttgta aaggcaaaag tattaaatga tatagcagtt taatttaatt
    12901 ttaacaaata caatttttat tataatttat aatataactt acaaatagct atttactatt
    12961 taccataatt aagatggttt agattagtca aatagctatt tttttagttt gagtaaatat
    13021 tttaaattta aataaatact tcaattttaa aataaaagca atatttaaat ttattatatt
    13081 atctacaaaa agtaggtgtg ttatgttgag tttatttaga ttttattggc caaattggag
    13141 gtgatataac tccatatcca cgagctattg tatctttact tggattgatg attttatgtg
    13201 gtacattagc ttttataaat gttgagtcac ctggatttaa tctaactacc ttttcaccca
    13261 tacaaatatc aaccacacca ctcgttacca ccataatttc ttctgatgca tgatagatat
    13321 aatctttagc atcctcactt tctggtttaa gttcaaactc tacaaataaa attgaaggtg
    13381 taaattcagt tgtaggcatt ggagatacta tctcgtaaaa catttcactc tttggaaatt
    13441 tcatcataac tctatcttct tttctaatta ctaaatcatc agtattattg tcatccataa
    13501 acatatataa aggagtgtct aaaacttcag cgatttttct caatgaagac aaggatggtt
    13561 caatagcatc tctttctatt tgagagatat aacttataga taaatctgtc aatttggaaa
    13621 catcacttaa tgtttttttc ttgctttttc ttatatttct catcttttca cctagcataa
    13681 gaacctccaa ataataaaat taatataaaa tattcgctta aaacaaagta attatagcac
    13741 aacatagaag tagtggcaat aagaatacat agtagatttt gacaattaaa atttaattaa
    13801 atccaaataa aagtctatga aaatgaatta ttgttaaatt aatataaaga aaataataat
    13861 aaaaaaattt aatagaagag tattaattga attaagatta tcacattata ttttggaaaa
    13921 gagtaaattt tcagtaaatt atcttgacat atatattgaa gtaataatat aatttaaagt
    13981 ataaaatttt aaaaattgaa ttataacttc aaaaaataag gagggtaata tgaagataac
    14041 agatatcaaa tttgaaaagc ttagaattaa attaaaaaaa cctgtagtag tttcatttgg
    14101 agtaattgaa tatggagaaa gtgtaatttt aaaaattgaa actgatgaag ggtattgtgg
    14161 atttggcgaa gcagcacctc ttgcagcagt tacaggtgaa gttttggata atgttctttc
    14221 agtattgctt atgtttaaaa aagagttaat aggcaaagac ccactagata ttgaaaccat
    14281 acatacaatc atggatggaa ttattatagg taatacttct gcaaaagcag ctattgacat
    14341 agctttatat gatattaaag gaaaaattat gaatgcacct ctttataaag ttcttggagg
    14401 atttgatagt aaagtccaaa cggatattac tataagtata gataagccag aaaaaatggc
    14461 agtagaagct ttagaaagag taaaagaagg ttttaggata ttgaagctta aagcaggtat
    14521 aaatcctgaa gacgatattg aagctgtaaa attaattaga gaagccgttg gagagagtat
    14581 aagaattaga attgatgcaa atcaaggatg gaacgtaaat agcagtataa atactatcaa
    14641 aaaattagaa gaatttaatg ttgatgcaat tgaacaagca cttccacatt gggatttaga
    14701 tggtactgct tacataagaa ataaaagtaa tacaaaaatt atgatagatg aatcgttaca
    14761 ttcaccaata gatgcaatta aggctataaa aaagaatgca gttgatacat ttaatattaa
    14821 gctgatgaaa tcaagtggaa tatatcctgc tataaaaatc aacaacatag ctgaggcaag
    14881 tggagtaaat tgtatgttag gatgtatgct ggaaacgaga attggtataa cagcagcagc
    14941 aaatttaata gcatctaaaa agaacataac agaagctgac cttgatagtt ttatgttttg
    15001 tgaagaatca aaagatatat ctggaggatt tgtaatggat ggagatatta tgaatcttgt
    15061 caataaacca ggtttaggaa tagaagtaaa cttatgataa atttggaggt taaatatgaa
    15121 tacacttttt tcaaatccag gtagtatact tgctgtaatg acatcaatga ttgcattggg
    15181 tttttactta caaagataca aagccattaa aagcttagga ccagcactta caataatcat
    15241 aatgggaata atactttcaa acttaaaagt agttccagta agtactgagt tatatggaac
    15301 tatttcaaca tatgctatcc cagtttctat gactataatg ttgatgagtg ttgacttaaa
    15361 agaaatgaca aaattatcaa gagaaccttt aattgcaatt tttgtagctg tattaactgt
    15421 aagtatcatg gcatttttat ttggattagt atttgcagaa aaaatatcag aaggttggaa
    15481 ggttgctgga atgtttgtag gtacatacac tggtggaagt gctaatctta cagctattgg
    15541 aacaggcctt aatgtaagta gacaaactct tgctgcagca aatgcagcag actatgtaat
    15601 tggagtacca acattgatat ttatgtttgc tctaccagca atattaaaaa attcaaagaa
    15661 gtttaaaaaa ctttggccat atcatgtaga agaaacagaa ttagaagatt gtcaaaatga
    15721 agaatttatg gagtcaaaag aatggagtat taaagatata gcttggatgt tggctatagg
    15781 ttttgttgtt acagaagttt caacaatact tgctggatat ttcaattcaa gttttagtag
    15841 tgcagcaaga atacttttag ttacaacaat ttcaattata attgctcaat taaagccagt
    15901 aaagaaacta aagggaaatt tagatttagg attatttgtt gcattattct ttttatgtac
    15961 aataggtttt tcagtagata taaaagagtt tttaggttca acttttacta taacacttta
    16021 ctgttttagc ataatttttg cttcatttgt ttttcattta ggcataacta gacttttaaa
    16081 gataaagtat caatatgtaa tactttctat agtaggagca attgctgatg gtcctacttc
    16141 agctttagta gctgcttctg caaagtggaa ttcgcttgta agtgtagcag tcgtaatggg
    16201 agtaattggt ggagtattag gaaattatgc aggtatatca gtggcatatg caataaagat
    16261 gtctctagga ttataggggg gagtttaatt gataaaattt aaattataca tatctggact
    16321 atacagtgga aatgtaatct ttgatggcga tttattaatt gaaaagttga atccttttac
    16381 aaataaaata gaaagtttaa aaccaataag caaagaagaa aatacatact atttaaatct
    16441 tacaaaaata gatttaaagt ctttatttaa taactttgat gtatatacta aaagcttagt
    16501 aaacacagat aaagacacag ttataaataa cttaggagaa acgcattcta aacttaatga
    16561 atatatatgg gttcaaagaa acaaaaagtt cccactagat ataattattg tagataataa
    16621 aatagtaggt tttatctgtt tatccagaga aacttgtact atattaatta tggatggata
    16681 tgaagagtat actgtattaa aagaatggga aaaaacccat aaaaatgaag agatatattc
    16741 tataagattt ggtggaaatt atatgattga tatgaaagat ggtataaaac tttctacaga
    16801 tgtatatctc cctgattttg tagattctac caaaaaagca ccaacaattc ttatgagaac
    16861 tccatatgga aaagaaaatg ataaagaaat atattataaa tatgtacaaa gaggatatgc
    16921 tgttgtaata caagatgtta ggggaagaaa tgaatcagaa ggtaagtggg aacctatgat
    16981 tcatgagagg gaagatggag atagtaccat aaattggata gtatcacagg aatggtcaag
    17041 tggaatagtt ggaatgcttg gagcatcata tcttggttat gtccaatggg cagctgcatc
    17101 ttcaggcaat aagcatctta aagctttagt tagtatagta acatcaggaa gcccttttat
    17161 tgatatacca agaaaaggag gagcttttgt atctggtatg cttgcatggg catttatggt
    17221 atcaagaaat aaagttgata gaagtaaaat ggtaagagat gattgggatg atgttttaaa
    17281 catacgacca atagaaaata tacctgttga agctcttgga tatagaattg aatttctgga
    17341 agaatggtta aagagagtag aaaaagatga gtattgggat ttgatggatt ggcatttaca
    17401 aaaagataaa ataaatgtac ctgcattggt tgtatctggg tggtatgatg ataatagtat
    17461 gggaactaca gaggccttag atgttataaa ggactatgaa aaaggaaaga gaaaggctat
    17521 tctaggtcca tggatgcata attcaaatac tttaagagat ataaatggta tttctttagg
    17581 aaatagttca ttaagatatg atttggatta taattatcta ttatggtttg ataaatactt
    17641 aaaaggtata gaaaataaca tagatacgac tgctccagta gagtattact ctgtaggttt
    17701 taataagtgg aagacagaag aaaattggcc aataataaat aagatagata aaagtatgta
    17761 tctaataagt gatggaaatg ctaacacatc tttaggaaat ggtagattag tatttgataa
    17821 tgatttagaa gaaaagtatg atagttatat atataatcca aaagacccat cagtacaact
    17881 aattgatatg tcagagaatg aagttggagt tccaaataac tacaaagatt tagaaaaaag
    17941 aagtgatatg ttgtgttata catcagatgc ttttagtgaa gaatttactg taacaggtga
    18001 tataaagtta gagttttttg catcaagttc tgccaaagat actgactggg ttataaaaat
    18061 tatggacgtg gatttagatg gaaattcaat taaattagct gatggtatac ttagtgctag
    18121 atttagaaat agtttttata agtcagagtt tatggaagaa ggagagattt ataagtttac
    18181 gattataact tcaaaaatat caaacacgtt taaagttggt cataaaatta gactagatat
    18241 aacgtctagt gctaaaaact ttattttcca aaatagcaac acgaaagagg gatataatag
    18301 tattgaatat atagaagcaa agaatacgat ttaccatgga ggtaaatatc catctaaatt
    18361 gatattgcca atagaaaata agtaaattta tatagattaa atagtgagga ggtgtctcaa
    18421 aatgaatttt ttagttcgtg agacactttc ttttatacga aatcaaatat attttcatca
    18481 tttcaacaat gaaaaagagg cttatctctc tgttttgaga tagcctccta taagctaaat
    18541 tgattatttt gtagctgcaa caacgctcat ttcaactaac aaatgctctc tagcaagtct
    18601 agcttcaaca caagctctag caggttcaaa tcctttttca acccaagcat cccaaacaga
    18661 gttcatagct tcaaagtctt tcatatctct taaataaata gtaacagata gcaaatgttg
    18721 cttatctgaa ccatatttgt ttaataaatc ttctatttta gctaaaactt cagttgtttg
    18781 ttcaattact cctccttcag catgagtttg tccacataaa taaactgtat tgttatgaac
    18841 tacagctcta ctcattcttc cagttccttc atatctcttt atttccatat tttaaatacc
    18901 tccatagtta agaaatctat taaaaatcta aataataaat taaaaaaagt ctatactata
    18961 taaaattata ttatatattc tgataataat ccataaaaac atataacttt ttagctataa
    19021 ttattattgt tattaattga aaagacttat tagttacttg acatagttta cacttttaat
    19081 tatagtataa attggaatat ttagataatt attatttaat gtataagcta agtttttaat
    19141 taatatggct ttatacattt atatagaaaa gaggtacgta tgagtagcac aatcaaatca
    19201 aatcgaataa aaggtataat gtttataata gcatcagcgc ttggctttgc aatgatgtct
    19261 gcattcgtaa agctagctgg agatttacca agttttcaaa aagtgttctt tagaaatctg
    19321 gtatcagcta taatagcttt ttggcttatc ataaaacata aaggaagtct aactggtaaa
    19381 aaagaaaatt taaaagtact tttatatagg tcaatatttg gaactctagg agttatattt
    19441 aactactatg caattgacaa gttgatgctt tcagatgcta atatgctaaa taaaatgagt
    19501 cccttccttg tagtaatatt ttgtgcaatt gctctaaaag aaaaaataaa tgtaaagcag
    19561 ataggagcta ttataattgc atttatagga gctttattca taataaaacc aacatttagt
    19621 gtagaagtaa ttccatatct tgggggaatt gcaggagctg tatttgcagc aatggcatat
    19681 acttgtttaa gagttcttgg agatagagaa gattactata caattgtatt tttcttttca
    19741 gttttttcat tagttactgt agggccaatt gcttttgcag tatatgaacc aatgaccctt
    19801 atgcaactta tatatctatt actaggtgga gtatttgcaa gtctaggtca atttggaata
    19861 actttagcat ataaatatgc acctgctaag gaaatctcta tatttgacta tagtaatata
    19921 atcttttcag caattttaag tatattctta ttcaatgtct atccagatat gttaagtgtt
    19981 gttggatatt taatagtatt ttcggcagca ttttatatgt ttttatacaa taagaagtta
    20041 gataaattag acaaagaaaa gaagaagtaa actagataaa agtgttttta taatttgtat
    20101 tgaatattta agtctttgta ttaaaattaa agaaaattta attaaaaatg tttggtatat
    20161 taacttataa cagcagttaa aattgaaaaa ttataactgc tgtttctttt attaaaaata
    20221 tccaaatgtc tgtaaaaata gactataata gagtagtata aataatataa aggggataaa
    20281 ttaaagtatg gaaaaagtga aagttgctgt gattggagca ggtaatagag gaacgtatgc
    20341 atatgctcca tatatttatg aaaattctga tatatgtgaa atagttgctg tagcagaacc
    20401 taaaaaagga agacgagaac tatttacaca aaaatataac ctagatagta aaaatgtatt
    20461 tgaaacttta gaagactttt ttaaacatga caagatggca gatgcagtaa taatagctac
    20521 aaatgatgat agacattatg atgtagcaaa actagctctt gaaaaaggtt atcatgtact
    20581 attagaaaag ccaatgtcta atagtttaga tggtttggta catatagatg acttgtgtga
    20641 taagtataaa gataagattt ttatgatttg ccatgtactg agatattctc cattttataa
    20701 taaattaaaa gaaattgtag aaagcaaaaa attaggagag ctagttagta tacaatataa
    20761 tgaaaatata ggatattggc attttgcaca tagctttact agagggaatt ggagaaatag
    20821 caatgaaacc agccctttaa ttttagcaaa gagttgtcat gacatggata ttttgttata
    20881 tttagtagga agtagatgca aaaaaatatc tgcatttgga agcttaaagc atcttaataa
    20941 ccaaaatgct agtggagaga tggcacaaaa ttgtcttcag tgtttagttg aaaaaaaatg
    21001 tccatattca gcaaagagaa tttatttaga aaaagataga agtataaata gagctgttca
    21061 cataaatcca actgaagaga atctattaaa tatacttaag acaagtccat atggaagatg
    21121 tgtttacaga tgtgataata atgttgtaga caatatggta aatatattac agtttgaaaa
    21181 tggagtgacg gctactttta atttatgtgc atttactaaa gaaaatggaa gaactataaa
    21241 gttaatgttt agtcatggtg aagttggtgg ggatttaaat aaaaatgaga taagaattaa
    21301 aaaatttgga aaaaacgaag aaatagttat gaatccatca aatcaacaga atatggtaga
    21361 ggaatatgat agaaatctta tagctgaatt tattaaatta gtttcaaata aagaactaga
    21421 aaagggtaga gtagcagcaa aagaagctat acaaagtcat gtaatggcat ttgcagcaga
    21481 atattctagg gtcagtgatg aagtagtgta tatagaagaa ttttttgata gtgctaagca
    21541 aatgactaag gagatagaag aaactatatt ttaattaata aaaatttatt aaataaaata
    21601 aagatagatt aaataataga aatacgaaaa tatgtgttct attaaggaat ctatcttttt
    21661 tattaaggtt ttttcttatc cctatcaaaa aaatccatac attctgataa aagataagaa
    21721 agtaaagcga ataagatttc tgtacttaaa ggaatcgtta ctgaatccat aaaggtatca
    21781 attatgttca tagctagaaa agttagtata aaatcaatta taaattcgca aagtttatat
    21841 attgagtatg gtaatttgtt tacatatctg aatatatcta aaatagtagt gcatagaaaa
    21901 tcaattggag tggttataca aaaatatatt aaaaagaaaa ttaatacagc tccaagagat
    21961 ttatattcaa atcctaagaa tgataagaag ccaattccta taaccgtgaa aatacaaaaa
    22021 gttataccaa aaaaaacagc aaaaaataaa aatacagaaa taaattcgcc taaaggaatc
    22081 cttcttttat tacttatctt aatcaactcc ttaatttatg atatgaaatt tatataaata
    22141 ataacattct taaaaattca taatagtcct ctgtttcaaa tagtactgtt tgagaatcta
    22201 tttttttcat atttggataa aaagaggcct taaaagcttt ataatgttct ctaaatttta
    22261 tttctatctt aaatttatct ggcaatttta ttaagtgcct agataaatct ccagataaag
    22321 cttttttgac acctttttca atttttttta gtgcaaggtt tggatggatt gaaattgaac
    22381 catttccaat tccttttgac acaggaacag tgactatatt tggatttagt ttttttgcac
    22441 tctcacaaag catttcatct ccacttaaaa atgctacagg aacattataa tatgcagcaa
    22501 tataagcatt tatagtaaat tcagatgcta tttcatcatt tattttaaaa tagtcataat
    22561 caatatccat agtatgagaa agtggattac cattacaagt agctccagag tgatagccgg
    22621 tgaatataca tgcatcaaag ctttcatcaa ttccagccat cataactagt gggtctttgg
    22681 tccatcctct aagtattttg acgttttcag gtaaaatatt tggattaata tttctaccac
    22741 tgtcatgtgc atctttaaca agtatttcat gtacaccaga ttgagtagct ccatcacatg
    22801 ctgctgttat ctctttactc atttgtgttc tatagtgttg tgaaaattca cattcaagtt
    22861 ttgtttcatc ccaatttaca ataccacaag taccttctat atcagcactt aaatacaatt
    22921 tcataatata taccccctta tgtttattat tgatgataat aacagataac agaaaataca
    22981 taatcaataa agggcataaa aatattatgt cttactatta attattatta aatctgtaac
    23041 tgaaaaataa ctaaattggt attaaaaaat ataacactat gtgtcattta tttatatagt
    23101 tttttatatc tgatgtttat ataaaattgt aacatataag tggttacatt tactagttta
    23161 acatagttaa aatagttagg tattattttt aagttaaatt gattattata aaatcgtaga
    23221 ataaaagaaa aatttaattt attaaaatgc atgaaatgtt ttttcctcaa cataatgcta
    23281 aattaaactc aaaaagaaat aatagaaaaa tcttatgtag cttagaaaga atgtgaatga
    23341 tttaagggaa aaatagaaat ttattatata atattaggct gtattatgta atacagccta
    23401 atattatatg tatatgtgac ataaaatctt ggtaagtgta aataaaataa atgtggaaaa
    23461 caaattaatg aaatcaagaa aaaagtaatt taaaattcaa tttcatcttc ccaattaaat
    23521 tctcctgatt tttctacact cttaggccag tgactacacc atttaggaac ggacggattt
    23581 tcaaccctat caacgcttgg agtatatggc tttaagtcta taacaggact attatcatta
    23641 gcatcaatat atggtatttt tattatacca ttttcatggt cgatattgat aatttgtaca
    23701 gcagtcaatg ctattggatt gggtcttata ggtgaccttg ttgcaaatat tcctataacg
    23761 tttggtccac ttttataagg ttttggggtt tctaaaatac ttcttagttc aggattatca
    23821 aacccatcag cccaccaaaa tacatttaaa tgactgaaat cgtcgagttc atttaatgca
    23881 ggtacatact ttttgtctaa tttaataaag acctcttcgc cttgaataca tattttacct
    23941 ataggattta ttaatatttc tttcataata ttacctccaa ctatttaatt tatcatttgt
    24001 aattttaggg tataacatga catcatgtga gggtcaatat aaaataataa ataaaaatat
    24061 taatagttag aatgataata cattttttac atggagcatg acaccagtaa taaggcaatc
    24121 ttcgtcaata tcaaaagtag aggtatgcaa aggggaagta atgttttttt cacgatttgc
    24181 acaaccaagg tggtaaaaag ctccttcaca gtgtcttaga aaatacgaaa agtcttcagc
    24241 cccaagagtg ggcgagtctt taaggataat attttcttct ccaaacaatt ctacagcatt
    24301 ttgcttgacg taatctacta gatggtttga attgataaga gctggataac cttcctcaat
    24361 ttcaacagaa atacttcctc caaatgtttt acaagtaaaa tcgcaaattt ctctaattcg
    24421 tttttttgaa aactctctag tttcaggagt caaggttctc aaagtacccc ttataaccac
    24481 tttgtcacaa actatatttt ccttgattcc accttcaatc tttcctagag aaataaccac
    24541 agaatcagtt ggatttgtgt ttcgactaac gattgtctgt agagctgtaa taatatgagc
    24601 agatataaca atagcatcaa taccttgatg tggataagct ccatgacatt ttgaaccatg
    24661 tacagttaat tgaagtgtat cggtggaagc atttagagtg ttgtatttta attcaattag
    24721 atttttgttg atattgggat ttacatggag tccaaatata tattttacat ttgggttttc
    24781 aagacatcca tcttctatca taagttgagc cccaccaata gtttcctcgg caggttgaaa
    24841 aaagaatttt acatttacat ttaagtaatt ctttaattta aatagtatat tacaagtacc
    24901 cagtaaaata gcagtgtgtg catcatgacc acatgcatgc attttaccaa tattaataga
    24961 tttataagaa taattaagtt cttctgttat aggtaaggca tctatatcag ctcttatagc
    25021 aactgtttca aagtttggac tcacgttaat gtatgcagtt atccctgtat gattcttata
    25081 ttcaatataa tttataccta tttcatttaa gtatttaata atttttttct tagtttgaaa
    25141 ttcttctaat ccaagttctg gagtcttatg taaatctctt cttatattta taatccaatc
    25201 atttatctca tgacaaagag ttcttatatt aagcataaaa cacatcctaa ctatattaaa
    25261 aatttatttt tcttcattaa ttatattatt ttatgtaaat cattattatg tatcaagtac
    25321 aaataaaata tcttataatt atattagaga tatttagtat ggagaagaat atatcatttt
    25381 ataagaagag gtgtttaaat gttaggaata attgcgccat ctgggccatt aagaaataca
    25441 agtttggagg aaataaagtt taatttagaa agttatggtt acgaagttaa gttttctgaa
    25501 agttgtagtt taaattataa aggttattta gctggtaatg atgatattag agcaagggat
    25561 atagaagata tgtttttaga taaagatgta gatataatta tgtgcttaag aggaggttat
    25621 ggaacaacaa gaatattgga taaaataaat tatgatataa taaagcaaaa tccaaaacca
    25681 tttataggtt tttctgatat aacaggttta aatttagctt tttataaaaa ttgtggactt
    25741 ttaccatatc atggaataat ggcagcaaat gtaggtaaat gggataaatt tacctataaa
    25801 tcattggtaa atgctttaga atttaaagat gacttatatt tagaaaatcc aaaagaagaa
    25861 aagatttata ctgtatgtga aggtaaagca gagggaataa taatgggagg aaatttatcc
    25921 ttaataattg ccacaatggg aacaaaatat gaaatagatg caaaagataa aattttgttt
    25981 attgaggaaa ttggagaacc tcaatataaa ctggatagaa tgttaacaca actatattct
    26041 tcaggaaaac ttgaggaatg taatggtata atttttggag attttaagga ttgtattgaa
    26101 gaaaatgatt tgatggaatt actaatagaa tttgcaaata aggtcaataa accttctata
    26161 tataatcttc agtcaggaca ttgtatccca atgataacta ttcctttggg cagaatgtgc
    26221 gaattagatg ctacagaaaa aatcgttaaa ttaaaaaaat agtgtgtata taaatataat
    26281 agaaatatgg tataatctta tacaagtaaa aatttagcta ggaggatgaa aaatgtctga
    26341 tgatagaata atagacttta acgaactaaa gaacaaagtt aaagattctg atgtagataa
    26401 atttgagcaa tacatatata acttatattt ttctgttatg gatggaaaga tgtccatggc
    26461 tgagttttca agaaaaatat tcgattatat gagagataac aacatatctc aagaaaaatt
    26521 tatgaaaata caaaagcaat ttatggagag atatggtatg gacactgaag aagtagaaaa
    26581 acaacttaga aactttggaa tcgacccttc tacagcaggt tttatgagta ataatacttc
    26641 cagcaaagta tctacagaag atttagagtc atttaagaag agtgcaggat tttatgagaa
    26701 atatggagaa aaaatccaac caaagagttg tataacaaca tttataaaaa atgacttaaa
    26761 tgatattaat gtaataatcg accaagaaaa gataatgctt tgtagtgata gaaaaataaa
    26821 tcttatggat tcagaactaa atgagttttt attagaatat aagaatatgt ttaataaaaa
    26881 aataaaggta gtaatgtgcg aaactacgaa taagtatgat tattaaaaaa tgaatttaaa
    26941 tactgaagta gtatcaatta attgtactac ttaaaaataa aaatcgcaat ttattattcc
    27001 ttataaattt gtataaggaa tatctaaact gcgatttttt atattgtgaa gattagtcat
    27061 aatagatagt gttttaaatt gtaatcttct cactaaaact gtgtttttct attagaaatt
    27121 ttattaaaaa taaatatctg ataattgtat tatatgggat gtaaagtatt agaggcttaa
    27181 cgaatctctt ttaatacatt aactccattt tgaatcatac tgttgaaaaa tactaagttt
    27241 aaaatctcag cagggtgaat gttaggaata tcataagtat ctactaaatt cataggtaca
    27301 actatatttt tttcaatatt atgttgatta aaatatgatt ttagagttat tgcaaattga
    27361 tatatgcata tatctgtgca atcgccaact ataattatat tatctatatt atcaaaatcc
    27421 aaattttcta atgcaaaaaa tccattagta gaatttttag gaagaatttg taggttttct
    27481 atcgacttaa gttcatcaac caattcactt tctacatcat tttccaaaca gtgaacagga
    27541 taactcaaaa gttctattga tttaggagta tgagaatcag taaatgctat tactctattt
    27601 aatttgtttg atattttttt tgtaaacatt tcaattggtt ttattaaaga ctctactcta
    27661 ggagagtata atgcaccttg tcttgcaaag ccattattaa catcaattat aaataaagca
    27721 gttttactca aatcataatt ttctattttt tctattggta gattatctaa attactcttt
    27781 aaagcttcaa gttcatttaa taagttatcc atagttatac ctccaagata aatttaataa
    27841 aattgtacat attaattata taaaagtcaa ggagtacaaa ctattgtata attagctgta
    27901 ttgttttgag tgtaaaagat ttgaagcaat atctggatag ttagtaaaaa taccatctac
    27961 attgagtttt agacagtgta gtatatcatc ctcttcattt acagtatata cattgacacc
    28021 aagtagattt ttgtgacaaa gttcaattag agattcattt actaaaacta gaggtggatg
    28081 aaggtaacta ttacccagaa atttagcaaa attgactaca ttgtttatag gtaaataaca
    28141 caataatcct gtttttacac tagggtctaa tttatgaaag ttttttaagc tattgctgtt
    28201 gaaactagat ataagaactc tatttttcat attatacttt aaaataagct ctaaaacgtc
    28261 tttttctata ttgggatagt ttattttgtc agtttttagt tctatattta aaataaggtc
    28321 tgtaggtgaa attagaatta atacttcttc taaagttgga attttacatt ctttattgtt
    28381 ttgatatcct tcaaaagatg agtttagagt ttttaatttt cttaaagtaa agtcttttac
    28441 aaaaccatgt ccattaaaag ttctatcaac cttttcatca tgaataacta caatttttcc
    28501 atccttagtt ctgtggacat caagttctat tccatatata tccatataca aacatttttt
    28561 aaaagctaga atagtatttt ctggatactt tccactaaac cccctatgag cataaatatt
    28621 catttatatg gttccctcct ataagtataa cttatcttat aaaaatttaa ttaatataat
    28681 atatcatatt ccaaaaggta ttgttatata ttagttaaat aactatttgt aatttatata
    28741 ttat
//
LOCUS       DAEPOG010000030        28476 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEPOG010000030 DAEPOG010000000
VERSION     DAEPOG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.030"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..336
                     /locus_tag="KR432_001164"
     CDS             <1..336
                     /locus_tag="KR432_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0639713.1"
                     /translation="KVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKE
                     AGIDSEITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYY
                     GILKSVYKK"
     gene            352..1935
                     /locus_tag="KR432_001165"
     CDS             352..1935
                     /locus_tag="KR432_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0639714.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1963..3291
                     /gene="ssnA"
                     /locus_tag="KR432_001166"
     CDS             1963..3291
                     /gene="ssnA"
                     /locus_tag="KR432_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH0639715.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3490..4842
                     /locus_tag="KR432_001167"
     CDS             3490..4842
                     /locus_tag="KR432_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0639716.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5034..6143
                     /locus_tag="KR432_001168"
     CDS             5034..6143
                     /locus_tag="KR432_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0639717.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6426..7817
                     /gene="hydA"
                     /locus_tag="KR432_001169"
     CDS             6426..7817
                     /gene="hydA"
                     /locus_tag="KR432_001169"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0639718.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7811..10372
                     /gene="xdh"
                     /locus_tag="KR432_001170"
     CDS             7811..10372
                     /gene="xdh"
                     /locus_tag="KR432_001170"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH0639719.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10524..11870
                     /gene="rpoN"
                     /locus_tag="KR432_001171"
     CDS             10524..11870
                     /gene="rpoN"
                     /locus_tag="KR432_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH0639720.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12667..13689
                     /locus_tag="KR432_001172"
     CDS             12667..13689
                     /locus_tag="KR432_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0639721.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13749..14756
                     /gene="gap"
                     /locus_tag="KR432_001173"
     CDS             13749..14756
                     /gene="gap"
                     /locus_tag="KR432_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0639722.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14978..16180
                     /locus_tag="KR432_001174"
     CDS             14978..16180
                     /locus_tag="KR432_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH0639723.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16206..16949
                     /locus_tag="KR432_001175"
     CDS             16206..16949
                     /locus_tag="KR432_001175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH0639724.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16958..18490
                     /locus_tag="KR432_001176"
     CDS             16958..18490
                     /locus_tag="KR432_001176"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH0639725.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            19078..20370
                     /gene="eno"
                     /locus_tag="KR432_001177"
     CDS             19078..20370
                     /gene="eno"
                     /locus_tag="KR432_001177"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH0639726.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(20490..20918)
                     /locus_tag="KR432_001178"
     CDS             complement(20490..20918)
                     /locus_tag="KR432_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0639727.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            21003..21509
                     /locus_tag="KR432_001179"
     CDS             21003..21509
                     /locus_tag="KR432_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0639728.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            21627..21851
                     /gene="secG"
                     /locus_tag="KR432_001180"
     CDS             21627..21851
                     /gene="secG"
                     /locus_tag="KR432_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH0639729.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(22235..23173)
                     /locus_tag="KR432_001181"
     CDS             complement(22235..23173)
                     /locus_tag="KR432_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0639730.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(23161..23559)
                     /locus_tag="KR432_001182"
     CDS             complement(23161..23559)
                     /locus_tag="KR432_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0639731.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            complement(23983..24480)
                     /locus_tag="KR432_001183"
     CDS             complement(23983..24480)
                     /locus_tag="KR432_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH0639732.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            complement(24586..24948)
                     /locus_tag="KR432_001184"
     CDS             complement(24586..24948)
                     /locus_tag="KR432_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639733.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            complement(24966..25580)
                     /locus_tag="KR432_001185"
     CDS             complement(24966..25580)
                     /locus_tag="KR432_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0639734.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            26176..28311
                     /gene="rnr"
                     /locus_tag="KR432_001186"
     CDS             26176..28311
                     /gene="rnr"
                     /locus_tag="KR432_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH0639735.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT        11268 a         3344 c         5264 g         8600 t
ORIGIN      
        1 aaagtttctt atgcagttga aaaagctgat tgctatacag gaaatactat agaaagtgaa
       61 agattcttcc caggttggtt atatgatgaa gaagatgaat ttgttcaagc agcgtataat
      121 ggattaaaag aagctggaat agattcagaa ataactcaat actcattctg tacgaatgga
      181 agtcactacg caggtgaggc tggaataaga actataggat ttggtccttc taaggaaaat
      241 cttgctcata caatagatga gtatatagaa ttagaccaat tattcaaagg tgcagaagga
      301 tattatggta tattaaaatc agtttataaa aaataattag gagatggttt tgtgagaact
      361 ttaataaaac atgggcttat agtagatgga aataaaacac cagcatatga aggagatatt
      421 ttaatagaaa acgagaaaat attaaaaata tcacaagatt taaacgaaga agctgacaaa
      481 gttatagatg caaaaggtag agttatatgt ccaggtttca tagatactca tagccattca
      541 gacctagtta ttttagttaa tccatataat gaggtaaaga ttagacaggg aattacaaca
      601 gaagtgttgg gccaagatgg tatatctatg gcccctctac ctcaagagca tatatcttct
      661 tggagaaaaa atttagcagg tctagatggt gaaagtgatg aaattgattg gaaatatgaa
      721 acaacagaga attatttaaa aatgatggat tacaatgggg taggtttaaa tgaaacttat
      781 ttagtacctc atggaaacgt tagaatggaa gctatgggac ttgaagatag acctgctact
      841 aaagaagaaa ttcaaaaaat gtgtgaaatc acagagagag aattaaaagc aggagctata
      901 ggattgtcta ctggacttat atatatacct tgtgcatact cattgactga agaaattata
      961 gagatgtgta aggtagtagc aaaatatgac ggagtatttg tagttcacca aagaagtgaa
     1021 gcagatacaa ttttaacttc aatggaagaa atcatagaaa ttggaaaaca atcaggagta
     1081 aaagttcatt tttcacattt taaggtttgt ggtaaagcaa actggaagta tataccacaa
     1141 gttatagagc ttttagaaaa agcagaaaag gaaggaataa gagtatcttt tgaccaatat
     1201 ccatatgcag caggaagtac aatgcttgga gtagttttac ctccttgggc acattctgga
     1261 ggaactgata agttaataga aagattaagc gatgaaaacg aaagagcaaa aatgaaaaaa
     1321 gatatagcaa atggaataga aggctgggat aattttatag aatttgctgg aatagaccaa
     1381 atatttgtaa caagcgtaaa aacagaaaag aataaagata caattggtaa gagcttatta
     1441 gaaattggaa aaatgagagg aaaagaccca ttagatgcaa cgtttgattt attaaaagaa
     1501 gaagaaaatg cagttgggat ggtagatttc tatggattag aagaacatat aataggattc
     1561 atgaaaagag acgagcaaaa tgtgtgtaca gatggtcttt tagcaggtaa accacatcca
     1621 agagcttatg gttctttccc taaaatacta ggaagatatg ttagagagtt gaatgtacta
     1681 acaattgaag aagctgttta taaaatgact aaaaaggcag ctacaagctt tagtataaaa
     1741 gatagaggcg aattaaaaga aggatatttt gcagatatag ttatatttga caaggataca
     1801 gttagtggtt gtgatgattt tatcaactct atgcagtacc ctacaggtat agattacgta
     1861 attataaatg gaaattgtgt tgttgaagaa ggaaaataca ataaaattaa agcaggaaaa
     1921 gtacttaaaa attaaatctt agctttaagg aggaataaat ttatgatttt agtggggaat
     1981 gggaggctaa taagccaaga ttctttaaat ccctatatgg aagatggatg tgtagtaata
     2041 aatgataatc taattgaaga tataggtact acagaagaaa tgaaagctaa atatccagta
     2101 catgaattta tagatgcagg caagaaaata atcatgccgg gtctaataaa ttcacatatg
     2161 catatataca gctcatttgc tagaggaatg gcagttccag gaaaaccttc tgagaacttt
     2221 ttagagatac taaataattt atggtggaga cttgacaaac agcttacact ggaagataca
     2281 aaatacagtg catatgcaac gtatatagaa tgcataaaaa atggtgtaac aacagtttat
     2341 gaccatcatg caagtccaaa tgcaattgaa ggcagtttgt ttacaatatc agatgttgca
     2401 aaggatttgg gaattagaac gtgtctttgt tatgaagtgt ctgatagaga tggtggaaat
     2461 acaatccaac aaggtatcaa tgaaaacgta aactttataa aatacgctca aaaagatgat
     2521 acagacatga taaaaggaat gtttgggctt catgctgcat ttacactatc tgatgataca
     2581 ttaaataaat gtgcttcaga gatggctggt cttgatacag gttaccatgt acatgttgct
     2641 gaaggactag cagacttaca atataatgta gataaatatg gaaaaagggt tttagaaaga
     2701 ttcaatgatt ttggagtact aaaagaaaaa acaatagcag ttcattgtat atatataagt
     2761 gcagaagaaa tcagtctttt aaataaaaca aatacatttg tagttcataa tccagaatct
     2821 aatatgggaa atgcagttgg atgttctcct gcaatagagt tattaaaaag gggagttaca
     2881 gtaggattag gaactgatgg atatactagc gatatgtttg aatcattaaa agttgccaat
     2941 ataattcaca aacatcactt aaaagaccca agagttggat ttgaagaatc acaaacaatg
     3001 ttatttgata acaatagaaa aatagctaaa gcttattata aaaatgacct aggagtactt
     3061 aaaaaaggtg cttatgcaga tgttatagta gttgattata taccacatac tccattgaca
     3121 gaaaatacaa tagggggaca cactattttt ggaatgagtg gaagaagcgt agatacaact
     3181 attataaatg gaaaagtact tatgaaagat agaaagttaa taaacataga tgaagaaagt
     3241 attctagcaa aaagtagaga attaagtcaa aatctatgga atagaattta aataattgat
     3301 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattaat
     3361 atacaagtaa gaaacaagaa tttagatgtt aaaaacagag cttaaacaaa aataggaatt
     3421 taaatgaagt atataaataa aaaatatacg gaatagaata ttaaattaaa tatagaaggg
     3481 agacccaaaa tgtctcaaga taaaagccct aaaatagctc cagtagatga aaggttacct
     3541 gtatccaaat cttgggtatt ttcactacag cacgttttag caatgtgtgc aggagcagta
     3601 gcagttccta tgatgatagg aagtgctgca cgactagatc atgctagtat tgttttttta
     3661 ataaatgctt gcttgtttat ggcaggtata ggaacactca tacaaagtgt tggtatagga
     3721 aatattgtag gtgctaaaat accagtaata gaaggaacta gttttgccgc tgtggcaggt
     3781 atgacagcaa tagcctcatc atatagtgat tcacaagttg caatacagac agtatttggc
     3841 tctgtaatag tagctggatt gttttgtata ttacttgctc cagtatttga aaaattaata
     3901 agatttttcc ctagtgtagt tactggaaca gttgttatgg taataggtat ttcactattt
     3961 ccagttggga taaaatggat aactgacaac aaattggaag cggttgaacc aaactcatta
     4021 gcattagctt tagcagtatt ggcaataact ttactttcat ttaaattctt aaaaggaatt
     4081 tggaatagca cagctatatt ggtaggtata gtacttggta cactactttc catggcagtt
     4141 ggaatggcag attttagtgc agtaaatgga gttggttggc ttaacttaaa tgtaccttta
     4201 aaatttggaa tgcctagatt tgaaccatct gcaatactat cattgtgttt gattatgttg
     4261 gtacttatga cggaatcagt tggtaatatg atagctattc atgaaatggt agacaaagaa
     4321 gtaacaggta aaaatattaa aagaggtcta tttggagatg gaatatctac agcattatct
     4381 ggaatattca attcatttcc aataactcca tttggtcaaa atacaggaat agtaggtctt
     4441 acaggtataa aatcaagatt tatagcagtt tacgcagggg ttatattgct agtacttgga
     4501 ttcttaccaa aatttgcagc aataatagga gcaataccta agcctgttct cggaggcgta
     4561 ggatttgcaa tgtttggaat ggtaatggtt ggaggagtaa aaactttaag caaagtgaaa
     4621 tttgatggaa ctaaaaatgg ggttatagta gcagttagtg ttggtttgtc tatgatacca
     4681 ctaacaaatc ccgatttcta tcataatttc ccaacttggg tacaaacaat attccatagt
     4741 ggaataacaa caggtagttt atcagcagtg cttttaaaca tattcttcaa tgaactaggt
     4801 tctaaaaaag aaaaaaataa taaagttgaa gaagcaaaat aaaaataaaa caagtaaaaa
     4861 tctatattga cttaaagtca ttaagcatta ataaaaataa ttcagagata ttaataattc
     4921 aacaaaacta tcttatattt taaataatat aaaaaagtaa ataaataacg aatacaaata
     4981 aaagaataca atctaaatat taaaaatatt taatttaagg gagagataat aatatggcta
     5041 atttaaaagt taatttatat ggaatggaat ttttaaaccc aattatgaca gcggcaggac
     5101 ctggagcaaa agatggtgct ctctgtcaag aggcagcaaa aggtggagca ggagctatat
     5161 gtacaaaaac tatatctgta ttaccagctg atgtaccaag accatgtatg gcaaaaacta
     5221 atagtggttt tttaaacaca gaattatggt cagagcttcc aaaagagcaa tggatagagg
     5281 aagaatacaa aaaggtaaaa gaaacagggc ttccagtaat agtaagtatg ggatattcag
     5341 cagagcaaat agctgaggtt gctcctttag taaagcctta tgcagatgca gttgaattat
     5401 ctactcatta tgtaggtaca gacgttgcac caatagtaaa tgctttaaaa gctgcaaaag
     5461 ctgttttaga tgtaccagta tttatgaaaa tgagtcctca tactgacatt caaactattg
     5521 caaaagctgt tgaagaagca ggtgcagatg gacttgtaat gataaactca tttggaccat
     5581 gtatggcaat agatgtaaat acaggatacc ctataatggg aagtaaagct ggatacggat
     5641 ggttatcagg tgcaccaata agacctctag caataagatg tatatatgaa gcttctcaag
     5701 ttgtaaatat accaataatc ggtgttggtg gaatcacttg tggaaaagac gtggctgaaa
     5761 tgatgatggc tggagcaagt gctgttcaag tatgtacaga agcaatcctt aaagggcctt
     5821 ctatctatgg aaaaattgct aaagaattaa atgaattttt agatgagaat ggatatgctg
     5881 atgttaatga aataaaagga ttaactcata agaaaatggc agctagaggg tttagaactc
     5941 attcagtagc tccagtagtg gatgataaca agtgcatcaa atgtaagcaa tgtgtaacaa
     6001 gctgtgtata cgaagcttta gaagttacag ataagttgaa catagatgca gacaagtgtt
     6061 ttggatgtgg attatgtgta actagatgtc caaaaggagc tttaagcatc tgctacaatc
     6121 aagtagaagc tatgaatgag taataattat atagaatttt ggtaattaac ttagagtaat
     6181 aggtattagg ataataaaat atatcagata aacaaacaca cattatgcat taataataca
     6241 gtctaaagtt aataatttta ttatattgat ttaactaaat attagtttag ctgaatatta
     6301 atttaattag gtattggttt aatcttaata tagatttact acgtataagt gaactaacta
     6361 gattacttta agttaatttt ttcatataaa ataaattgat acattacaag taaggtggag
     6421 tctatatggg aattgtttta aaaggtggga aagtagtaac aagtgaaaaa acttactgtg
     6481 cagatgtaag aatagaagat gaaaaaatag tcgatatagg tcttgatata tcaaaagaaa
     6541 atgatgaaat agttgatgtt agtgattctt atgttatacc aggaggtata gatacacaca
     6601 cacactttga cttagataca ggcgttacta agacagcaga taactttgag acaggaacaa
     6661 aagctgctat agtaggagga actactacta tattggattt tgctacacaa gctaaaggat
     6721 gttctttgaa agacggatta gaagaatggc atgaaaaagc taaggataaa tcctattcag
     6781 attatggctt tcatatggct ataacagatt ggaatgactc ggtttgtaat gaaatggagg
     6841 atatggtgaa agagggagta tcttcattta aactgtatat ggcgtataaa ggaagccttc
     6901 aagttgatga tggtgtaatc tttgaagcat tgagaaaagc agaagagata ggagggatta
     6961 ttggtttcca ttgtgaaaat ggggacataa tatgtgaact tgtggataaa gcaaagtcag
     7021 aaaataattt atctccaaaa tatcatcaat taactagacc agctataatg gaggcagaag
     7081 ctacatcaag acttatgaag atagctgaag ttgcaaactc aaaagtatat gtagttcact
     7141 taagctgtaa agaatcctta gaggaggttt taaaagctaa gcaaagaggt gtggatgtaa
     7201 tagttgaaac ttgtcctcaa taccttctat taaatgatga attatatgat ttagatggat
     7261 ttgaaagtgc taagtatgta atgtcaccac ctttaagaaa taaagaaaat aatgaaatat
     7321 tatggaatgc tattttagat aatcaaatag acactatagg aacagaccat tgttctttta
     7381 attataaggg acagaaggaa cttgggataa acgatttcag taagatacca aatggttctc
     7441 caggtgtaga acataggatg agtcttttat acacatatgg agtctgtaaa aataaaatat
     7501 ctatcaacaa gatggtagaa gtaacatcaa ctaatgcagc aaagttgttt ggaatgtatc
     7561 ctaataaagg aactatagaa ataggcagtg atgcagatat attagtctta aatccaaaca
     7621 aaaaatccaa aatagaagca aaaaaacaat tacaaaatgt agattataca ccttatgaag
     7681 ggtatgaagt ggattgtcaa atagaacatg tttatttaag aggaaaagaa gtagttagag
     7741 atggagaatt aatttgtaat actccaagtg ggagatatat aaataggcgt ctaacaaagg
     7801 gggctagaga atggtagaat ttatattaaa tggagagaaa gttgttgcaa aagaaggcaa
     7861 aaaactttta gacttcctta gagaagatat gaatataact tcagttaaaa atggctgttc
     7921 agaaggtgct tgtggaacat gtatggttat aatagatggt aaagctacta aagcatgtgt
     7981 atttaaaact gataaattag ctgggaaaaa cataacaact atagagggat tatcagagag
     8041 agaaagagat gtttttgcat attcttttgc tagtcaagga gctgtacaat gtggattttg
     8101 tattccaggt atggtaatta gttcaaaagc acttatagat gttaataata atcctactga
     8161 agatgatata aagaaagctt taaaaggtaa tatatgtaga tgtacagggt atgtaaaaat
     8221 aattaaagct gtcgatttag cagcaaagat ttttagagaa aatatagatg tgccaaaagt
     8281 aaactgtaag ggtctagtag gtgaaaatct tcctagagtt gatgcagttg gcaaagcact
     8341 tggatattct gaatatgtag atgatatgag aattgagggt atgatttatg gttcagcagt
     8401 tagagctaag tatcctagag ctttagttaa aaaaatagat atagaagaag caaaagctta
     8461 tccaggagtt gttgcagttc taacagctaa agatattcca ggaactataa aggtagggca
     8521 cttaaagaag gattgggata ctttaatacc agaaggtgaa ataactagat atttaggaga
     8581 tggaatagtt ttagtagcag cagaaactag agaagcgtta gaagaggcca aaaagctagt
     8641 taagatagat tatgaggaat taacaccatt aagtactaca gcagatgcac ttagagaaga
     8701 tgctccaaag atacatgaga cagggaatat acttgttaag gaacatttag taagaggaaa
     8761 cgctaaagaa aagatagaaa attcaaaata tgttgtaact aaaagatata ctacacctgc
     8821 tacagaacac gcatttttag agcctgaatg tgcagtagct ggtccttatg aagatgatgg
     8881 agtaataatc tattcaacag accaaggggt gtttgccact caacatgaat gtgcagatat
     8941 gttagggtta ccatatgaga aggtaagagt tataaacaaa atggtaggag gagggtttgg
     9001 tggaaaagaa gacatgagtg ttcaacacca tgcagcctta cttgcatacc atacaaagag
     9061 atttgtaaaa gtacagttaa caagaaaaga aagtattatt gttcatccaa aacgacatgc
     9121 cacagaaatg gaatttacga cagcatgtga tgaaaatgga tacttaacgg gtatgaaggc
     9181 aacgataata tctgacacag gtgcatatgc ctctcttgga ggaccagtgc tacaaagact
     9241 gtgtactcat gcagcaggac catacaatta tcaagatatt gatataacag gaacagcagt
     9301 atatactaat aaccctcctg gaggagcatt tagaggattt ggagttacac agagttgttt
     9361 tgcaactgaa tgtaatctaa atttacttgc tgaaatggtt ggaatatctc cttgggagat
     9421 aagatataaa aatgcaataa gacctgggca agttttacca aatggtcaat ttgcagatga
     9481 aggaacagca atggcagaga cactagaagc tgtaaaagaa gagtatgaaa agcatccacg
     9541 tgcaggtata gcttgtgcta tgaaaaatag tggggttgga gttggtatac ctgatgttgg
     9601 aagatgtaga ttggtgataa aagatggcaa agttcacata agaacaagtg cttcttgtat
     9661 aggtcaaggg cttggtactg ttatgactca agtagtttgt gaaactgcta gcctacttcc
     9721 tgaaaatata gtgcatgaag ttccagatac tcatttaaca ccagactctg gtaatactac
     9781 ggcttcaaga cagacagtat ttacgggaga agctactaag gttgcatcaa ataagttaaa
     9841 agaagcttta gactctggca aaactttact tgatttagag ggagaagagt tttatggaga
     9901 atatgaaagt attacagata aaatgggttg tgacaaagaa aatccagtaa gtcatgttgc
     9961 ttatggatat gcaacacaag ttgtaattct ggatgaaaat ggaaaactag aaaaagtagt
    10021 agcagcacat gatgttggaa gagctataaa tccaatcagt ttagaaggtc aaatagaagg
    10081 tggagtagtt atgggtcttg gatatgctct aaccgaagat tacccagtag ttaactctgt
    10141 tcctacagct aagtttggta cattaggttt atttaaatct acagatatac cagaaataca
    10201 agctttaata gttgaaaaga acaaagattt attggcttat ggggcaaaag gtgtaggaga
    10261 aatttgtaca gttcctactg caccagcagt acaaaatgcc tattatgtat ttgataaaga
    10321 atttagaact gagctaccac tacaaaatac tccatatagt aaaaaaaagt aacaaaaaaa
    10381 agaaatatta aataaaaaaa tagatgaaat tgtaaataaa aaaaaccctt ttgcataagg
    10441 tatattttaa atgatagaat atacatatac aggagggttt ttcttttata aaataagttt
    10501 aataaaattg agaggtgagc agaatgaatt ttaataatag tctagaatta actcagtctc
    10561 agaagctaat aatgactaca caacttaagc aatcattatc aatattaaat atgagtaagt
    10621 tagagttaga agaagagata aaaagagaag cagagaataa cccattagtt gaagtcgaga
    10681 aaagtgggga gataaattgg gaagagtata taaaagacat ggataggtca agacgtttag
    10741 atagaacaga gataagctat aatcctgata atgaagttaa tctagaaaat ttagttaaat
    10801 attcgtcaaa tttatatgaa gatttaaaat ttcagataag cctgtataaa cttacagaca
    10861 aagaattgga agtctgtgag tatataattg atagtttaga tgaggatgga tatttaagaa
    10921 ctgaggaaaa agtaattgtt gataccttaa agatagatga agaacttttt gaaaaatgtt
    10981 taattagtgt tcagcagttg gaacctagtg gagtaggggc aagaagtctt tcagaatgtc
    11041 tgatgataca aatggctagt ttaggaatat ataatgaaat tttagaggaa atcgttacaa
    11101 aagacttaaa tttaattgca aataataaat ataaagagat aagtaaaaag tacaatatgt
    11161 cattacaaaa atgtgttgaa ctaataaaca taattaagac attagacccc aaaccaggaa
    11221 gaacttgttc agtagagaaa agtgtataca ttcaacctga tgtaacagta gaaaagattg
    11281 atgatgaatt tattgtatat ttaaatgaga aagattctta ccaaataaga atcaatagtt
    11341 attataaaga catattaaaa aattcacagt ctgatgaaag tgcaaaagaa tttataaaag
    11401 aaagactaaa ttcagcaaca ggacttatga aaaatataga gagtaggaaa actacagtac
    11461 ttaagattgc agaagagata gtaaaagctc aagatgaatt tttgagaaaa gggacaaaat
    11521 atataaaacc tttgaaaatg aaagacatag cagaaaaatt agaatttcat gaatctacta
    11581 taagtagagg tgtaaatggt aaatatatgc taacaccttt tggcgtatat gaatttaggt
    11641 actttttcag tagcgccatt gagactgaga ataatgaaat ggcatctagt acaagcataa
    11701 aaaaaattat aaaggaaaca ataaaagacg aaaataaaaa gaaaccttta agtgatgacc
    11761 atatatctaa gttattaaaa gaaaaaggaa tcaatgttgc aagaaggaca gttgccaaat
    11821 atcgtgaaga attaggaatc ccatcttcaa gtaagagaaa agaatactaa taagtaaaag
    11881 acaaatatca gtatggtaag gggaaaaggc taattaagag atgaatgtag attgggctaa
    11941 aaattttata aagtaaaata atagaatatg gaaaagtatt aaaatgctat aaggtattaa
    12001 aaaactgggg aacattaaaa cattaagata gcaaagaaca taaaaaacta aagaagatta
    12061 aaacattaaa gaacattagg ctttgaggaa tattagaact ttaaagaata ttaaaattta
    12121 taaaatataa aaatactaaa aagaattgaa aaactgggga acattaaaat attgtaaaat
    12181 gctaaaaaac tagaaaacat aattaaataa gtgcttataa agaatgatta aattaaataa
    12241 gtgtttgtaa agaataatta gatattagaa aaagaagaat acctagataa ataaactatt
    12301 attaaattta ataatcacaa aaggaaaaca cgtagggtat aaataaaaat cttaactaga
    12361 taaaagtaaa cttagttgta gataaaaata actagaaata aaagtaacta gaaataaaat
    12421 tatttatagt aataaatatt agaatgaaaa tataaaaaaa tatatataaa aagccttata
    12481 taaaaagaaa aaatactgtg atttgaaaat ggtggagaaa tattttataa aataaaaaaa
    12541 taaaaaagtt tttgaaaaac tattgaaatc tatacgcaaa tcctataaaa taaaaatatc
    12601 ttgtgggact taaataaaca ctagggacaa aaaaagtccc gaaaggaaag caataaggag
    12661 tatgttatga agaacttatt agaaattcaa aagaaattga ttccacaggc aattgaactt
    12721 atggaaagaa gatactctat tttaagacaa atatctttaa gtgaaccaat aggtaggaga
    12781 acattatcaa atgtattaga gattagtgaa agaataatca ggtcagaaac tgagttttta
    12841 aaagaacaag gacttataga cgtagctgta tctgggatga ctatcactaa agaaggttct
    12901 gatttgttag ataaacttaa ggacatcatg agtgacatca tgggattgtc taagttacaa
    12961 gatagagtca gagaaaagct tggaataaaa agggtaatac tagttcctgg aagctgtgat
    13021 gaaaatggaa gtttactaaa agatgtaact agatatggat gcgagtattt cttaggcatt
    13081 ttaaaagatg gagatatcgt ttcaataaca ggaggaagca ctatgttgga gttttcaaac
    13141 gttattaaaa ctgataagag atatccaaac tctacaatag ttccagctag aggaagtatg
    13201 ggaactgatg tagagatgca atctaacaat atagttgctt caataagtaa gaaattacat
    13261 tctaattgca aacttttaca tataccagat gagttggatg aaagtgcaat gaaaactctt
    13321 agccaagtgc cagagattaa gacaactttg gagtatatta aaagaacaga cattttagtt
    13381 tttagtatag gtagagcaga tgtcatggca aagagaagaa aacttccaga agacaaggtt
    13441 gatgagatat tatctaaaaa tgcagttgga gaagcatttg gatattattt caacaaagat
    13501 ggagaaatag tttacaaact gaacactgtt ggaattgatt tagaaacagt taaacaagta
    13561 aaagaaacta ttgcaatctt tgcagggact gaaaaagtag aagccttatt agctatatca
    13621 aatatgcata aaaacatggt tcttgtaaca gatgaagaaa gtgcttataa gatattgtca
    13681 ttattataaa agtcaagacc tagcacagag ctagaaaaca tacatttact ttataggagg
    13741 tagcgaaaat ggttaaagta gctataaatg gatttggaag aataggaaga ttagcattaa
    13801 gaaaaatgat ggagcaacaa gataagttcg aagttgttgc aataaacgac ttaacagacg
    13861 ctaaaatgtt agctcattta tttaaatatg atacagctca aggaagattt aacggagaaa
    13921 tagaagttaa agaaggtgct tttgtagtaa acggaaaaga aataaaagta acagcagaaa
    13981 gaaatcctgc tgacctacca tgggctgaat taggagtaga tatagtatta gaatgtacag
    14041 gattctttac atctaaagat aaagctgaag ctcatataca agcaggggct aagaaagttg
    14101 ttatatctgc accagctact ggagatttaa aaacaatagt attcaacaca aacagtgaca
    14161 tattagatgg ttctgaaaca gttatatcag gagcttcttg tactactaac tgtttagctc
    14221 caatggctaa agtattaaat gataaatacg gtatagaaaa aggattaatg actactatac
    14281 atgcatacac taatgaccaa aatactttag atggtcctca tccaaaaggt gatttaagaa
    14341 gagctagagc tgctgctgga aacatagttc caaacactac aggagctgca aaagcaatag
    14401 gcttagtaat accttcatta aaaggaaaat tagatggggc agcacaaaga gtaccagttg
    14461 taactggttc aataactgaa ttagtttgta cattaggaaa aaatgttaca gttgaagaaa
    14521 taaatgcagc tatgaaagaa gcttcaaacg aatctttcgg atatactgaa gaaatgttag
    14581 tatcttctga cataatcgga ataagctacg gttcattatt tgacgctact caaacaaaag
    14641 ttatggaagt agatggaaaa caattagtta aagttgtttc ttggtatgat aatgaaatgt
    14701 cttatacttc tcaattaata agaactttag gatactttgc acaattagca aaatagtcag
    14761 ttttgagaaa atagaaaagt agattgacaa agtccgagtt tgtagtttaa tactacggac
    14821 tcaggacttt ttttaagaaa aaatatataa aatacaccta aataatgtta catactatat
    14881 ataaaaatat gataatatga tatatttaat agggtaaaag tatgtactat attatacttg
    14941 gcaataaaaa attactttat taaaaggaga gatatttatg tcaatgctta acaaaaaaac
    15001 tatagaagat attgatgtgt gcggaaaaaa agtattagta agatgtgact ttaatgtgcc
    15061 attacaagat ggtgtaatta ctgatgaaaa tagattgaac ggagctttac caactattca
    15121 atatttaatt tctaaagggg caaaagttat attatgctct cacttaggaa agccaaaagg
    15181 agaagctaaa ccagaattat ctttagcacc agtagcaaag agattatctg agatgttagg
    15241 aaaagaagtt gtatttgcag cagacgataa tgttgtagga gaaaatgcaa agaaagcaac
    15301 tgaaaaaatg gaaaacggag atgtagtatt attagaaaat actagatata gaaaagaaga
    15361 aactaaaaac gaagagaatt tctctaaaga actagcatca cttgctgaaa tatttgtaaa
    15421 tgatgcattt ggaactgctc atagagcaca ttgctcaaca gttggtgcag gcgagttttt
    15481 acaagagaga gtttgtggat acttaattca aaaagaatta aaattcttag gagaagctgt
    15541 tgcaaaccct gtaagaccat ttacagctat acttggagga gctaaagttt ctgataaatt
    15601 agctgttata aacgaattat tagaaaaagt tgataatcta ataataggtg gaggaatggc
    15661 ttatacattc ttaaaagctc agggttatga agtaggaact tctttacttg aaatagataa
    15721 agttgaatat gctaaagaaa tgatggaaaa agctaataat aagggtgtta acttattatt
    15781 accagtagac gttgttatgg cagaccactt tgcaccagat gctactccaa tagtaacaga
    15841 agatgctaat gtaaaagaag attacatggg actagatatg ggacctaaga ctatagcaaa
    15901 ctttgtaaaa actataaaag aatcaaaaac tgtagtatgg aatgggccaa tgggagtatt
    15961 tgaatttgaa aactttgcaa atggtacatt atcagtagct agagctatgg cagagttgac
    16021 tgatgcaact actgtaattg gcggtggaga tagtgcagca gcagttaatc aattaggatt
    16081 tggtgacaag atgactcacg tatcaacagg tggaggagca tcactagaat ttttagaagg
    16141 taaagaatta ccaggtattg cagcattaga taataaataa actaatatat agaggagatt
    16201 gtaaaatgag aaaacctata attgcaggaa actggaaaat gcataaaaca ataaaagaag
    16261 ctttagaatt tgtaaatgaa gttaaagata aagtaaattc tgataaagta gaagctgtta
    16321 tatgtgcacc atttacttta ttaaaggatt taaaagaagc tactaagggt acaaatataa
    16381 agataggtgc tcaaaatatg cactttgaag aaaaaggagc atttacagga gaagtttcac
    16441 ctcttatgtt aaaagaaata gatatggact atgttgtaat aggacattca gaaagaagac
    16501 aatacttcaa tgaaactgat gaaactgtaa acaagaaagt tttaaaagca ttagaagtag
    16561 gaatagaccc aatattatgt gttggagaaa ctttagaaca aagagaagct ggaaaaacta
    16621 aagatgtttg taaagttcaa gttgaaaaag ctttagaaaa tgttcttaaa gatgatttat
    16681 caaaagtagt tgttgcatat gagccaatct gggctattgg aactggtaaa actgcaactg
    16741 ctgaagatgc taatgacgtt atatcttata taagagaagt tataaaagga ttatatggag
    16801 aattagctaa tgaagttaga atacaatacg gtggaagtgt taaaccttca aatgtagccg
    16861 aaataatggg tcaaagtgat atagatggag ctttagtagg tggagctagt ttagctagta
    16921 atgattacct agaccttgtt aatttctaag gagtgaaatg atgaaaaaac cagttgcttt
    16981 aattattatg gacgggtttg gatacaataa ggatgttaag ggaaatgcaa ttgctgagtc
    17041 caaaactcct aatttagata gaataaaaaa agagtatcca aacacattga taaatgctag
    17101 tggtcttgat gttggtctac ctgatggtca aatgggaaat tcagaagttg gacacactaa
    17161 cataggtgca ggtagaatag tatatcaaga tttaactaga ataacaaaat ctataaaaga
    17221 tggtgacttt tttgctaata aagttttatg tgaagcaatg gataatgcaa aagaaaattc
    17281 tcttcatgta atgggacttt tgtctgatgg aggagtacat tcacatatag accatcttaa
    17341 ggctataatc aaaatggcaa aagataaagg cgtacaaaag gtgtatgttc acgcatttac
    17401 tgatggtaga gatactgacc ctcaaagtgc attagaatat gccaaagaag tacaagctag
    17461 tatggatgaa attggagtag gtgaatttgc tacagtttct ggtagatact acgctatgga
    17521 tagagataag agatgggaaa gagtagaact tgcatacaat gccatggtta gaggtattgg
    17581 tgaaaaagca aattctattg aggaagctat tcaaaactct tatgatgatg gaaaaaatga
    17641 tgagtttata atgccaacag ttattatgaa agatgataaa ccagttggaa gtataaagga
    17701 aaatgattca attatattct tcaactttag accagataga gctagacaaa taactagagc
    17761 tttagtttgt gaagagtttg atggatttaa aagagaagat attaaaaact tctttgtatg
    17821 cttaacagag tacgatataa ctatagagaa tgtgcatata gcatttggac ctcaatcttt
    17881 agccaacact ttaggtgagt atctagctaa aaatggaaaa actcaactta gagctgctga
    17941 aactgaaaag tatgcacatg taactttctt cttcaatggt ggagtagaag agccaaataa
    18001 aggtgaagaa agactattga taccttctcc aaaggttgca acttatgatt taaaaccaga
    18061 aatgtcagca tatgaattga cagataaagc tttagataaa ttaggagaag ataagtttga
    18121 ctttatagta ttaaactttg caaatcctga tatggtaggt cacacaggaa gtatagaagc
    18181 ggctataaag gctgttgaaa ctgtagatac ttgtgtagga aaattgatag ataaaatagt
    18241 agagttaggt ggaagtgcaa taataactgc tgaccacgga aatgctgaat atatgttaga
    18301 cccggaaaca ggaaaaactg taacggctca ctctattaat ccagtaccat ttattgtagt
    18361 tggtcaagaa tatgaaagtg caaaattatt ggatggagga agattatctg atatagcacc
    18421 gactgtttta gatatgatga agttagaaaa gccagaagaa atgactggtc attcacttat
    18481 ttcaaaatag tcgattctga taagtagaga ataatatata aactacaata taaatttaaa
    18541 aataatgcag tttaggttta ttttgtaaag tttagacttt atgacataag atttagattt
    18601 tgtaagttct ggatattgta aggtgtagat gaaggtatat tttataaaat ttacaagata
    18661 gacaatttat aaaataaaaa attataatat agataataat gtaaataatg atataaataa
    18721 taacaagata gacaataaca atataaataa taataattaa tatatattat ataagcaaaa
    18781 attttagaat cattagttgt acattttatg tttaatgttt taaatctagt tataatattt
    18841 gtgtatttaa gattggtttt ggattttaga caagtttttt tatattatat aaattaaaat
    18901 attataaaaa ataaaataaa aataggaact tttacaaatt tacccatcaa ataaatttgt
    18961 acctaatttt caaaagcaac tttatgtact ataatataga aagtagatta aaatagtaga
    19021 cggatgttta cagtaaatga aaatttaact aatagaaaat cgaagggaga tatacaaatg
    19081 tcagttattg aattagtata tgctagagaa gtattagact caagaggaaa tccaactgta
    19141 gaagttgaag tagtattaga agatggagca atgggaagag caatagttcc atcaggagca
    19201 tctacaggag cttttgaagc tgttgaatta agagatggag ataagggaag atacttagga
    19261 aaaggtgtag aaacagctgt tgctaacgta aatgaaataa tagcacctga aatagaagga
    19321 atggatgcat ttgaccaacc agctatagat gcaatcatga tagaattaga tggaactcca
    19381 aacaaaggta aattaggagc taatgctata ttaggtgttt caatggcagt agctagagca
    19441 gctgctgatg aaataggttt accattattc caatatttag gtggagtaaa tgctaaacaa
    19501 ttacctgttc caatgatgaa catattaaac ggtggagagc atgctgataa taacgttgat
    19561 gttcaagagt tcatgatatt accagtagga gcttgttgct ttaaagaagg tttaagaatg
    19621 ggagcagaag tattccattc attaaagaaa gttttaggag aaaaaggatt agcttgtggt
    19681 gttggtgacg aaggtggatt tgctccaaac ttaggttcaa acagagaagc tttagaatta
    19741 atagttgaag ctataacaaa agctggatat aagccaggtg aagatgttat gttaggactt
    19801 gacgttgctg ctacagaaat gtacaacaaa gaaactaaaa aatacgtttt agcaggagaa
    19861 ggaaaagagt taactgctgc tgaaatggtt gctttatatg aagattggtc aaacaacttc
    19921 ccaataataa ctatagaaga tggtttagac gaagaagatt gggacggatg gaagttatta
    19981 actgaaagat taggaaataa attacaatta gttggtgacg acttatttgt tactaacaca
    20041 gagagattag aaaaaggaat tgaaaatgga gtagctaact ctatattagt taaagttaat
    20101 caaataggta caataactga aactttagat gctatagaaa tggctaagag agcaggatat
    20161 actgctgtta tatctcacag atcaggagaa actgaagatt ctactatagc agatttagct
    20221 gttgcagtaa atgctggtca aataaaaact ggtgctccat caagaactga tagagttgct
    20281 aagtacaacc aattattaag aatagaagaa atggttggag aacaagctag atactgtgga
    20341 ttaaaatctt tctataattt aaaaaaatag ttattgaaga ttaaaaatat ggcattaaag
    20401 attaaaaaga taacataaaa gataaaaatt tatcttataa gggataagtg atagttgatt
    20461 tttcaattca cttatccttt ttttatattt catttcttac ttttattcac agctttagaa
    20521 aaaatacgaa taaaagtttc tatttcatct ttatctaatt gatgtaagag atttctaaat
    20581 acaattattt ctatgtctct atgttcttgt tgagccttta aacctaattc agttaagact
    20641 aagttatacc tccttttgtc tttaatacag ggattctttt taataaattt ttttaattca
    20701 agacgtttta cagcacttgt taatgtgctt tttggcaatt ctaaaatttc actaatttct
    20761 ttcatcatac tattgggata ctgttcaact atttctaaaa tacttaattc tactaaagaa
    20821 ataccatgaa tagtaggata cttaccacta cacatttctg ttttcatctg catactaaca
    20881 atatgccaca ggttgtttag ctcttttaca ggattcattg acttttacct ccatatatga
    20941 tacaattatc gcactaatat agtacgataa tcgtattaat ttgtcaatag gatggtgttt
    21001 ttatgaattt agagattata cgagctacaa gtgaaaatgc tatagaaatg ggttttatac
    21061 attcttgttc gtggagaaaa gcgtataaag gaattattcc agataagata atagatgaat
    21121 tcacttctga gaaaagagca gaagtttttc aaagtgttat tccaaaagct gaagaggaat
    21181 attatctttt taaagtgaat ggtatacctg ctggttttgc ttcattaaat agaagtcaag
    21241 aggataatgc tccaaaatgt gttggagaaa tatattctat atatttccat cccgattttt
    21301 ggggaacatc tgtgacaaaa aaaggattac aattttgcat tgatagatta agcaatttag
    21361 gatattcata tataacgata tgggttctaa aagacaatac tagagctata aatttctata
    21421 aaaaaaatgg ttttacttgt gatgattttg agaaagagat acatattgga aaaaaattat
    21481 tagaagtacg ttattcaaaa aagatatagt aattgcatat tatttttgtt tgttatttat
    21541 acttcaatag tcttgaaatg actatattat tgtgatacac taaagagtaa tgaaatttaa
    21601 gtaataaata gataggaggg gcaataatga gcaacatttt aatgggtgtg caagtagtgc
    21661 tagggatttt actagtaatt agcataatgc ctcaagattc aaaaaatgct ttaccttcag
    21721 aatttggtgg agaaggaagt caagcttact ttaaaccaaa aggaaaacaa gcatttcttg
    21781 ctagagttac aaagataaca gcagtattat tttttataaa tgcattggca ttacttatag
    21841 ttaagaagta gtgatattgt tgaataactg gccattattt aattagtgca tttgctagat
    21901 atttaaaaat taaatttagt ttttaataaa agtacatcaa cataaaaagt acgtaaaata
    21961 aaagctggac taatgatttt atatgattag tccagttttt attttataga ttgactattt
    22021 gttggcattt attatttgta tttatatatt gtttatattt atatattttt ttcaaactaa
    22081 aaaatatata gcaacactac tttcaccatg tttaatgaga taatatacac aaagttatat
    22141 tatatagtaa aaatagtata ataatatgtt tttacgcaat taaatcaatt ttatatatgt
    22201 ttctcataat accaatttgt gttatcaaat atcattaaat aagattattc tgctttaaaa
    22261 taaatattat taaaaatact gtaattatag aaaatccaga agtaaatact acaatttgtc
    22321 cagctaattc actgtcagaa tccatctgtt gtgccattgt aaatgaagaa actgcagttg
    22381 gagcagataa cattatcata agtgaagtta attctacatt tctaaatcca agaaaaatgc
    22441 taattggtaa aaatatagaa ggtacaagta taagttttcc acatacgccc aatataatgg
    22501 tttttatatg cccctttata gcagaaaaac taaaagatcc gcctagaatt ataagtgata
    22561 aaggagtagc tattttagaa atatcaccaa tagttttgtg taaaaatgta ggtatattaa
    22621 tatttaaaat tagcattgtc aatcctataa aagaggcgat tatgagtgga tttgttaaga
    22681 cgcttttaat catatgtctt atatttactt tgccacctct aaaaatttca agaattacaa
    22741 cagataatac attaaacata ggtacaataa ctgcaattaa taaagatgtg actccaatac
    22801 tcttatcgcc aaatagtgaa attgtcacag gaataccaaa tataacgaaa ttgctacgaa
    22861 atacaccttg tattaatgca ccccttgtct tattatcttt ttcaatttta ggaataataa
    22921 agactaagat taagaagatt gaaaaaataa ttataacagc tacagccata agcttaaaat
    22981 taagagaacc atctaaatct gtattgtata tattaaaaaa taataataat ggcaagaaag
    23041 acttgaagca gacattattc attttattta aagtatgagt atcaaataga cctaattttt
    23101 ttagaacata tccaagtgac attgtgagaa acaaaggaag aacaacattt attgatagga
    23161 ttaaattttc caaggtttac tctccgtgat tctatgattt gataattcaa ttaaatttcc
    23221 attagttttt ttcaaataaa tagatttcat attaccaaag acacctgtac attctacaat
    23281 tcctcgctta atttgaatct cattttgttc taagtatttt tttggctcat caatatttgt
    23341 gtttatttta aaacataagt tatctctact tgcttttaaa tttttaacat aaggtaaaaa
    23401 tttataacct tttatgttaa gatttatctt aacatttcta gccttaagtt tatacttttc
    23461 gctcatttct ttagatgaaa aaccaaattt tttataaaaa ttgatataat tttctatatt
    23521 attattagta atgaccacat gctctatttt ttttatcatg gcaactcctt aagattttat
    23581 ataaattagt taaatttata taagttatta aattttatat agattgataa tatttatata
    23641 aattactaaa ttttatataa gctattgaat tttatgcaga ttattgtatt tcatttagat
    23701 tataaaactt tatgtagagt taatgaattt aacatatatt attagttata ttaatgcttt
    23761 tattttaatg agtctaataa taattctaat agaaactgaa atagataagt aagttttaag
    23821 ctaaaatcaa tttatataaa ttgataataa tctaatagaa attaaaatac cagtcaacta
    23881 aaatatatat taagataaag tgcagaaaat attctaataa caattaagct aatcatcaat
    23941 taaatgtagc atttaattaa tgattagcta tagtaaacct aattaattag aatagcttcc
    24001 ttttagtgta aatgtatcta aaacatgccc ttcaatagct ttgaaaaatt cattcatata
    24061 gaatcctttt tcaaggtcaa ggatagtatc aagtgcaaat acatgtattt catagacatg
    24121 aggtctgtct ggaggagcca ttccaccata tacactggcc tctaatctgt caagacctcc
    24181 tagtttgcca tgccaactag tagtcccttg tacaaaatca gtagctgtta cactttcatt
    24241 ttcatttaac tcatcttttg ttatatttac agctaaccaa tgtatccatg caaacccaca
    24301 tacaggaaca gagtctttat cttctaaaaa tacagcaaag gatactgtat tctttggagc
    24361 atcttcaaac tttagaggta gtgaatatgt aggcatacca ccttcattga actgagttcc
    24421 tcttttaccg tatttatctt ctataacacc atttactata cctgtactag ttactttcat
    24481 aataatttac ctcctattat aataaattaa tagaatatat ctataattta attatatacc
    24541 agttgaaaat aatggtaaat tacttacttt tttgtgggta tctatttatt ttaaaacacc
    24601 tttttcctta agaatatctt ccataccatt atccaacata aagctaattc ctatatcttg
    24661 cattatagcc aaagcatcca atatttgttt ccctcgaata ggtgtaagtg agtattcaac
    24721 tttaagtggg taacctttat atgtcttttt atctacaaga ccaaattcaa ttaattcttt
    24781 aagttgttca agtaacatct tttgattaat accattgatt tccttttcaa gttttgataa
    24841 ggaggttggt cctatgcgta atctccataa tataatagat ttccatttgc cttttatcat
    24901 atcatgtgtt agttctaatg ggcaagtata ctcatcacga agtttcaaat aagttcacct
    24961 ccattttact ttgccaagat acattttgaa gcaagcttat atacaaactc aactttttta
    25021 tttgatatat cccatgaatc taattctttt tttactaaat ctggatattt tttacttata
    25081 tctctaagag cattacctac agactttctc aagtattcgc tatcatcttc ttttaaacta
    25141 gaaagtagtg atatagcaac gtcaggatta gatttaaaat agtctctact agtccaaatt
    25201 ctaagacctt ctgtcacagc cctttttatg tttggactat cagaattaag ccattctttt
    25261 ataacaggaa gtgccttttc atatcctact tcactgcaat aataatcaaa tgatttagct
    25321 agtatttctt gtactctcca gttttcatct ttactcacat tgtcttttaa aaataacaaa
    25381 gcttcagaaa tagttactga tataaaacca agtataaaca cagccaattc tcttacttga
    25441 taatattctg acctatataa gtagattgat agataataac acttttttga gtcattattt
    25501 tttacaattt ttttagcaat gttttcaatt tctttgaatc cattttctat agtaataatt
    25561 tttgcaatta cttcttccaa ttttatctcc tccaataaat agattagttt aaattttatg
    25621 tatttatttg taaaaaagtt tgtttaattt agacaaaata aagacttgtc aaaaaacgtt
    25681 tgcaagtctt tagtactagc ataaataaaa aataataaaa aatcaacctc atttaacata
    25741 taaatttact tttattataa gttttatatt ataaagttgt atttaaaaaa ttaattgagt
    25801 tatataaatt ttacatgatt tgagtatact aatagaataa attattatat agtaatactt
    25861 atatgagatg aatattttac caatcctatt tgatggtgta taattatgag ataaagagta
    25921 atttaagcaa ttatgataaa gaataactta agtaattata atgatattta caaataattt
    25981 ataaaacatc ataatttgtt tgaagttagt atgacaaagc ttacattaaa tcaaaaatat
    26041 aaataagtaa ttttaacttg taataataat aagaaaatat agaaaattat gtgataagta
    26101 aaaaatgttt tatctgaaga aatataaaag acaatataaa taataatata atgaaaagta
    26161 aaacaggagg gaaaaatgat accaggactt aaagaaagtt tgctaggatt aataaatgaa
    26221 agtgcatata atcctctaaa aaaagaagaa ttagcagaaa tatttgatat acattcatca
    26281 gaaatgccaa tgttttataa ttttttaaat gaattagaag aagatggtta tatctgcttt
    26341 actaaaaaag gaaaaatagt atctcccaat caaatgggat actttgttgg aaaatttgta
    26401 tcacacaaaa agggttttgg atttgtagaa tctgatgttg aatatacaca ggatttattt
    26461 atctcaagtg acaatataaa tggtgctatg cataacgata gagttatggc agaaatagta
    26521 gtacctgcaa ctgaagaaaa gagagcagaa ggaagaatag taaaagtaat aaagagagaa
    26581 ataactagaa tagtaggtac tttccaatca agtaaaactt ttggatttgt aacaccagac
    26641 aacaagaaat ttactaaaga tatatatata cctaaaaaac attttagtgg agcagtagat
    26701 aatgataagg tagtatgtga aattacagta tggccacaag aggatagaaa accagaaggc
    26761 aaaattatag agatactagg tcaaaaaggt gaaagaggcg tagaaataga ttctataata
    26821 agagaacatg gtcttccaga agaatttcca aagaaagtat tgcaagaagc agaagctgta
    26881 gctgttgaga ttcctcaaga agagataaaa agaagaagag atttaagaga tttaaacata
    26941 tttactatag atggagatga cgcaaaagac ttagatgatg ccatatcaat agaagtatta
    27001 cctaatggaa acttcaaact aggagttcac atagctgatg ttacacatta tgtaagagag
    27061 aaaaataaac ttgataagga agccttaaaa agagctacat cagtttattt agtagataaa
    27121 gtaattccaa tgttgccaaa aactctttca aatggagtat gtagcttaaa tccatttgaa
    27181 gataagttaa ctctttctat atttatggaa atagaccata aaggaaatgt ggttaaacat
    27241 gaaatatgtg aatctgtaat aaattcaaaa gctagaatga cttatacaga agtatctgat
    27301 atattagaaa aggatgatga aaaacttaaa aagacttttg aacatgttgt tgaagatttt
    27361 aaaaatgctg aaattttagc taagattctt atgtcaagaa gagaaaaaag aggagctata
    27421 gacttcaatt tcccagaggc aaagataatt ctaaatggaa aaggcgaagt tgtagatata
    27481 aagccatatg aaagaagaat atctaataag ataatagaag aatttatgct tataagtaat
    27541 gagacaattg ccgaacattt ttactggatg ggaataccgt ttgtgtatag aatacatgag
    27601 acaccatctt tggaaaaaat ggaagaattg agtaagttta tatctacatt tggatatata
    27661 ataaaaggtg ataaagagga agtacatcca aaagcattac aaggtataat agagaaaata
    27721 aagggcaaaa aagaagaagg agctatcagt actataatgc ttcgttcttt aaaacaagct
    27781 aagtactcac cagaatgtag tggacatttt ggattagcag ctaaattcta ctgtcacttt
    27841 acttcaccaa taagaagata tcctgacctt caaattcata gaataataaa agaatcttta
    27901 aataataaaa taagtggaaa aagacaagaa cagcttacta ctatagtgga ttatgcatct
    27961 gttcaatcat ccgagaaaga aagaaaggca gagttagctg aaagagatgt acatgatttc
    28021 tataaggctc tttatatgaa ggataaagtt ggtcaagaat ttgagggtgt tgtatctagc
    28081 gtaacttcat ttggaatatt tgttgagtta gataatacta tagaaggatt gataagactt
    28141 gcaaatatga atgatgatta ctatatttat gatgagagtt cttatagtgt tttaggagaa
    28201 agaactaaga agtcatataa gataggagac cttgttaaga taaaggttga aagtgttaat
    28261 gtagatttta aagaaattga ctttgagata gttgaaaaaa tagaggagta gataaaacag
    28321 ctaaaataag agataacaag ttgtagtatt tatgatatgt ataaatatat agtgcacaat
    28381 actctataaa ataaatgata aaaataggta attaagaaat atctaatgga aatagttaac
    28441 ttaaagataa ataattaaga aatatttaat gaaagt
//
LOCUS       DAEPOG010000031        27301 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEPOG010000031 DAEPOG010000000
VERSION     DAEPOG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.031"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     regulatory      50..135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            259..6222
                     /locus_tag="KR432_001187"
     CDS             259..6222
                     /locus_tag="KR432_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBH0639736.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     gene            6432..8312
                     /locus_tag="KR432_001188"
     CDS             6432..8312
                     /locus_tag="KR432_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBH0639737.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            8679..10280
                     /locus_tag="KR432_001189"
     CDS             8679..10280
                     /locus_tag="KR432_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBH0639738.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            10499..12088
                     /locus_tag="KR432_001190"
     CDS             10499..12088
                     /locus_tag="KR432_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBH0639739.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            12314..14590
                     /gene="slpA"
                     /locus_tag="KR432_001191"
     CDS             12314..14590
                     /gene="slpA"
                     /locus_tag="KR432_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBH0639740.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            14826..17171
                     /gene="secA"
                     /locus_tag="KR432_001192"
     CDS             14826..17171
                     /gene="secA"
                     /locus_tag="KR432_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0639741.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            17586..19457
                     /locus_tag="KR432_001193"
     CDS             17586..19457
                     /locus_tag="KR432_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBH0639742.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            19564..20271
                     /locus_tag="KR432_001194"
     CDS             19564..20271
                     /locus_tag="KR432_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0639743.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            20304..22139
                     /locus_tag="KR432_001195"
     CDS             20304..22139
                     /locus_tag="KR432_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0639744.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(22187..22582)
                     /locus_tag="KR432_001196"
     CDS             complement(22187..22582)
                     /locus_tag="KR432_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBH0639745.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            22809..25220
                     /locus_tag="KR432_001197"
     CDS             22809..25220
                     /locus_tag="KR432_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBH0639746.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            25517..27094
                     /locus_tag="KR432_001198"
     CDS             25517..27094
                     /locus_tag="KR432_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBH0639747.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT        11420 a         3052 c         5049 g         7780 t
ORIGIN      
        1 attgtattaa aaagtttaaa tatagtataa aatgggtaag aaagtagtga aaattaaatg
       61 gcaaaattag ggaaatctaa tgacgcaaaa ctatagggat taaagctttt aaagccatat
      121 cagccagttg ctaaaaagag taaagtcttt ttattttata attaagattc tctttgagtg
      181 tcttttttta ttgtctgaaa cttatagtat ttaaattcat gaaaaattag agatttatta
      241 agggaggatt gttgtttcat gaaaaaggca atatcttgtg tactagcagt atctatgtgt
      301 agtgcaccac taaatgtttt tgcagaacct atattagagg ggaagctaag ggcagttgaa
      361 aaatcagtta cagaaaagtt aagggggaat cttgaagtag atttaaattt ttcactgcca
      421 ataaaaaata gcgagtcaga aagtatgact aatatgcagt tacgattaaa agatgatagt
      481 aacaatagtg gaataataaa gcttggagaa aaagattcag gaacagtaaa tattgggaca
      541 gataatatag aatatactat aaaaaaatta tctgcaagta gaagtgaaat aaaaaataaa
      601 gatgagaatg tatcatatta taatattgta tttaataatt tacctgttgg aaaatacaat
      661 atagaagtct ctggagctgg atttaagaac aaagaaatta aagacattga tatatcaagt
      721 tattcacaaa gagtcttatt aagcaataga gcaagtgtag ataataaaaa taaaccttta
      781 aattatgaat tattcttaat gggtgatgta aatggagacg gtaaagtaga taagagtgat
      841 tataataagg ttttagagaa tatagattca aataaaagag aatttgattt aaatagagat
      901 ggaaaggtag atattgttga cttagattat gtacagaaaa atttaggaca aagtgaagat
      961 gcaaaaagcc tagaaagtat agtatctacc aatcctatag tagatacaag taaggttgaa
     1021 ttaaaaggaa atagtgatgt agatattaat ggtaatgtag aaaatttatt tagtggagaa
     1081 aataatggag ttacaatatc ttcaaaaaat gatgaaatat ctgaggaaaa accagcagta
     1141 atggatatag ctttatcaga agcacttaat atggaagtca tcaatataaa agctccagaa
     1201 ggtactgctc cagcaaaagg aatagttgag gttactgacg aagatggtaa tgttcaacag
     1261 attccttatg aaaataaaga atatatgaaa aacttaaaag ataaggagca acaagaggaa
     1321 gtaaataacg aacaagaaga agtaaatcaa gaagaagtaa ataataaaga tgaaaataaa
     1381 aatcagactg ataataattc aaataataaa gatgaaaata atgataaaca acaggaaaat
     1441 agcgataaac aagaagtaaa ctctaatacc aatcaagata aaaataatga taaagaaaat
     1501 tcatcaatgg ataaacctaa ttcagatgaa gatactccaa aagaagggga taatatcagt
     1561 aaagatgata actctacaac atctacatat agtagggcag cattagaaac atatacagat
     1621 gttcaaaata gcgatatagt aataaatctt ggtaaacaag tagcagtaaa aaagataact
     1681 ataaaaataa cagctacaac agcaaacgat agaaatcttg cagaaatatc aaaagttgaa
     1741 tttttaaaca atgtatataa agaaatacca aagccagaga tgaacatacc taaaataaat
     1801 aaaatatcga ctagcactgc tgttggaaat gaaaaagtat ctttttattg gaataatgaa
     1861 gtaaatgtat cagcatacga acttatacta gataaaattg atagtaaggg aaaagtatta
     1921 tctactaaaa aattacaaac tagtaaaaat acaatagaag ttagagatat agaagcatat
     1981 ggactatata gggttagcat acaatcttta aatggagatt ggtctagtgg ttataaagat
     2041 gcaacgccag aaaacttaga tggagtacca gaaaatgtag actcaaacta taaacctata
     2101 caatttagcc cagatagcat attagaattt caagttgttc cagacacaaa accagaacca
     2161 ccagaaggta taaatgttaa aggtaaattt aaatctcttg atgtaagctg gaaaaatcat
     2221 aaacaagcta aagactttga cttgtactat aaagaaaaag gctctaatgg gtcttggata
     2281 aaatacaatg ataagcttat tcgtgggaca agtttaaaaa tagaaggtct taaggataac
     2341 acaacatatg aagttagaat gacagctact aaccacttgg gaactagtgg aatgtctaaa
     2401 acatatatag gtacaactga aagcatagat gcaccaacaa cacctaacta tagattaatt
     2461 aatactccta aggagaatgg tggatttgga ataccaacaa atcacattgt agatgttgtt
     2521 tatccagcag gatatgacaa aagtgaatat agtgataaaa atccatttga taaattcaat
     2581 gtagtagatg gagacttcac aacacattgg acattcccaa catataatgc agctgaggga
     2641 acgaatagag gaccaataat aacttttgat aaatcttata ctatggatac ttttatggct
     2701 acacctcgtt ttgatgtttc tgataagaca ataaatgact ttgagatacg atattgggat
     2761 gaaaatggtc aattacacaa tctgggggat gttccttttg aaacaaagac agaccctgta
     2821 aataataata agtatgtaat ggttagatta aaagaaccaa taactgctac taagatacaa
     2881 gtaagtgttt gttcatctta ttcatataag tctaatatat cagaacttaa gttttatgag
     2941 tatgacagta tagaagatga tgtaagaaat ctatttatgg atgacctaca agttgaatta
     3001 aaagaagatg taactcaaga aaaaataact gaattaaaag atagattaaa tactccagat
     3061 aaagctagtg gagaatatca tccatataag acagttattg atagagaatt aaaattagct
     3121 caagacttat ataatgatag agccacactt actgatgtaa ctactgtaaa tcaagaaata
     3181 aataaatcag gaaaatataa agataaaaac ggacagtata ctgtaaattc caataactta
     3241 ggcatgcaaa atgactggca ggcattagga gtagctgcta gagcaggaga tgaaataaca
     3301 gtatatgttg gaagtaaaag tggaaaaact cctaagttga tatatacaca gtactatgga
     3361 gaatctggtg catataagag tggagaaata aatcttaaaa ctggaaaaaa tgtaattcaa
     3421 ttaagcaaac ttcatagttt agatatagaa tgtggaggaa gtttatacat aagataccct
     3481 gaagatactc caagtggagg agatataaaa gtacgtgtag caggagctac taagatacca
     3541 catcttaatc taaatggaac aataaatgat aagagtcctg cgggagtttc tgaatctaag
     3601 aaaaagataa aagcatatat agaagaatta gctaaatata aagatgctgt aaaaggagaa
     3661 ccatattatc caggaacaaa tattggtaat tcatatgaat attcagaaaa aactggtgtg
     3721 ttaaacacta cagatattga aagtgataaa gtaacattaa atgttcctgc aacagctgta
     3781 tatcaaggaa tatcaaaagg aaatgctgat ttaaatacac aagtagatag attatataat
     3841 agtatgcttg catgggaaca aataatggat ttagtttact cagaaagagg agtatttaaa
     3901 acacaagatt tagatggtaa tggaactgta gatgataatg aatttaactt aactaaaaat
     3961 gatatggctc caaaatctcg tatgaacata aaataccaaa gaatgtttat tggagcattt
     4021 atgtatgctt ctggattaca tgtaggagta gaatatggtt cagttccagg gcttatgaat
     4081 ggagtacctt tccaaataga tgatagtgga aaagctacag gaggaaactt atttggatgg
     4141 ggaataggtc atgaaatagg acatgttaca gatattggaa agatgacata tagcgaaact
     4201 agtaacaatg tacttgcttt acttgctcag acctttgatg ataagacaca ttcaagactt
     4261 gaaggttcaa caatggataa gatatatgag catgtaactt caaatagctt aggtatacca
     4321 agcaatgtat ttgaaaggct tggaatgtta tggcaattac atctagctta tgatgatgat
     4381 tttacgggtt caatgttaaa gaataactca gatgctgatt taagtaatga tacattctat
     4441 gcaaagattt caagaaaata cagagcactt tcaagtagtg acccaattaa tagtttacca
     4501 aaagaccaaa tgctagtagc aatggcatca agtgttgttg aaaaagattt aagagattac
     4561 tttaaggcat ggggagtaga aattacacca gaattaaaca gtataatgga tagtaagaac
     4621 tatgaaaaag aatcaagaaa gatacaatat gtaaatgacg aagctagaag aaagatttta
     4681 aatgaaaata tagctagtat ggcaaaagat actaaagtaa gtgctagttt ttctgatgga
     4741 atacaaagtg gttctctagt taaatctaat aaaatatcta tagacctaga tgtagataag
     4801 gataaagata agatactagg atacgaaata ataagaagtg atggaaatta tttagatggt
     4861 gaaaatggaa cacaggtaaa atacagaaca gttggatttg tggatgcaaa aggaaatagt
     4921 aaggccaaat ttactgatag tatatctcct cttaataaca gagcatttac atataaggta
     4981 gttgcatatg attatcacct aaatccaaca gaagaatttg aagttggcac tgtaaaatta
     5041 tcagatgaag ggaaaataaa taaatcagca tggtcattca taacaaatac tgtttctgac
     5101 ggtgatgtta gaactgaaaa tgactcacat ggaccattac aaaatcctga aattgataat
     5161 ataaaagata atgatgtaag tactacatat aaaggaaaga taataagtaa agcagagtgg
     5221 aacaaagacc ctcaaaaaga ccctgatata aatgtagaag aaaatccata tataatagta
     5281 gacatgaaag aaacattacc tatagtagga ctaaaatata caaaaccaga ggcagaaact
     5341 aggaagttct cattaaaagg attatttaac tttagaaaga atactagcac aacatacaat
     5401 cctttaacta attacaagat acaagtaagt aatgataaaa agaactggga agatgtaagt
     5461 caaggaactt ttgaatatgg acaagattta gttggtggag gaaaagacca agataatgaa
     5521 gctagagtta catttaataa agatgataaa ctttggacat accaagctag atatgtaaaa
     5581 ctaatctcaa acaataagtc taatatagaa gttgcagaat tagatataat aggacctcca
     5641 ggtgataata tagaaattgg cacaatgaat aatgcagacc aaactcgtac taatggaatt
     5701 ggaaagttag cagaagatta tgtatatcaa agtgatgacc ctagcacaag tgaagatgaa
     5761 acaggtaaga ttccagcagg ttcaataatt attacaggtg aatacagtgg aaatccagca
     5821 tttaatttac cactacttat agataaaaac aataagacta tatctggaga agcactttta
     5881 tttgcagaag ttccagaaaa aggtgaatta ggtgaaatca gtaaaggtac atgggtttac
     5941 tatatgccaa aagaaaactt tgattcttta agtgataagg ttaaggctga actatataga
     6001 tacaatgacc ttcaaggaga tacgccagta ggtcaaagat ttgtaagtga tacaatgtat
     6061 gtacctgtag gagctaagaa ttatgatgag ttaaagacaa taagtcttac tgatagtaat
     6121 agtaaagcta gaaaagctac aagaaatgct attgatgtca gtaataagaa ggttataact
     6181 gtaagcaata aagtaaaggg taatattgtt agagataaat agtaggtgga actctaaaaa
     6241 cgtaaaaata ctaatactaa cattgtatat ctaatatgat taggtactag aatagtttta
     6301 aaatagtaga tattaaaagc aaagtatata aatgtaacta taagtttagg gtaatttata
     6361 aattaaatat aaaagctagg tggataagta aatttatcca cttagttaat gatagataag
     6421 gacgggtgtt aatgagtaaa agaaaatctt ttataagaac tatagcagta tcaactatgg
     6481 cagtagctgt aactggtagt gttacatgtg catatgcagc tccagtttta caaggaacaa
     6541 aaacgtatga gaaagtaaat actatagata tatctgtgga ctcagtagaa aacatagtat
     6601 attcatttca agccagtata aaagtacaag gtgaggttga agtagtagat aatgagcaga
     6661 aagaaaagat tacttggtct gataatataa aatctcagat aaaaagtgga aatgcaaatg
     6721 ctacttgtag agtagaatac aacaaatcaa gtaatacaac tacattagat atatatgtta
     6781 catcaaatga agatttacta gacggaaata gattgaatat aggaagaata tctgtaaaaa
     6841 aatctggcag taactcaaat gctgactata aagttttagg aaaaggaaca agcgataaac
     6901 cagctttaaa aattgtaaca tataacaaca aaacagtgga ttatgaaaat ataaactctg
     6961 atgaaggatt aatatttacc cttattaatg agagtgaagt aaaaccaatt ggtggaactg
     7021 gttctagtaa aaatgaccct gagaagtata aggtagagaa aagtgaagca cttgaatatt
     7081 tattaaacaa tattaggata aactattcta ttgttagtaa agaaacacaa gaaagtggtt
     7141 caaatgtaat attaaaactt ggattagcac aaaaaataac taagggaaga aaagctacaa
     7201 taaataaata tgttgaagtt acacttccaa aaagtttaga atacatagtt gaaaatgaat
     7261 tatcaaaacc ggatgaatta ccaccagata atggcaatgg tggaaataat ggtggtggaa
     7321 gtaattcagg cggctcgtct agtggtggaa gttctggagg aggaagttca agtaattcta
     7381 catctaatgt cacagtcaaa aaattaaaag gtgctgatag atttgagaca gctaccaaaa
     7441 tatctcaaag tggttggaca aaatcagata cagttgtaat agtaaatgga gaagataaaa
     7501 gcatggtgga tgggcttaca gctacaccac ttgctagtgt aaaaaactca ccgattcttt
     7561 tatcatcaaa tgaaaagctt cctcaaaaaa ctgtagaaga attgaaaagg ctaaatccat
     7621 caaaagtaat tgtgataggt ggaaacaact caatgccaaa ttcagttgtt gaagctatca
     7681 aagctgtaaa ttcaaaaata tcagtacaga gaataggtgg agatacaaga tatcaaactt
     7741 ctattaatat agcaaaagaa atagataaga cgaataatgt aagcaaattg tatatagggg
     7801 caggaaatgg agaagcagat tcattatcaa tagcatcact ggccggtaaa gaaaaaactc
     7861 caatagtact tacacaaaaa gatggtgtag ataatgaagc tgtacaattt attaaatcaa
     7921 ataaggtgtc aaatatatac tttattggtg gaatagaaaa aatatctaac aaggctattg
     7981 aacaagttgg aaaaatagca aacaaagata tatcaaacaa tagggtggca ggtcaaacta
     8041 gacaagagac gaatgccaaa gtaatagata aattctactc tcaatctaaa ctagatggag
     8101 ttgtagttgc aaatcaagat aaacttatag atgctttagc agttggacca ttagcagcta
     8161 aaaacaattc accagttata cttgctacaa atacgttaga taaatcacaa gaatcatcat
     8221 taaaaggtaa aaattcatca aagttgtttg aagtaggtgg aggaatagcc tcatctgtag
     8281 tagataagat taagagttta attgaaaaat aaatattaag taaatcgaaa ataagaatgt
     8341 atttagatat aaagttatat atttagatgc attcttattt tattttcatt atcttaacaa
     8401 tttaataata ttgaggttaa aatgtataat aatttgagct atattaagtt taattttaaa
     8461 taaagcagat atcttttata tatataaata taaattcaat tttgaatatg tttaaaaaga
     8521 agttaaaaat aattttttaa atttaataaa ataaaacact aatatataga cgagatatat
     8581 tcatatcata tatactctat aatatatgga aaataaagag aaaataaata gaaaataatc
     8641 acaatataaa tataaaatat aaaaatcatc agggggatat gaaaatgaac aaaaaaatat
     8701 tatcattagg tctagcagta tcattaatct tagtaaactt caaaagtgta aatgcatcat
     8761 cagtagtaga aaaaatatac ggtaaagata gatatgaaac agcagcaaag atagctgata
     8821 gacagactta tgaaacagta attttagtaa atacagaaaa atcacttgca gatggattaa
     8881 gtgcaagtgg attatcggga gctacaaaag ctccaatact atttacacaa caaaataaga
     8941 taccagcaga cacaaataga tgtctaaaaa atatcaaaaa agcatatata ataggtacag
     9001 aagatactat aagtaaatca gtagaaaaag aactagattc taaaaatata gaagtcaaaa
     9061 gaattggtgg agaagataga ctaaaaacaa gctatttaat agctaaagaa atagcaacta
     9121 taaaaaaagt ggataaagta ctattaacta acgcatatag tggagaagca gacgcaatga
     9181 gtgtatcatc agtagctact agagatggag ctccaatcat acttacagat ggaaagagtg
     9241 tgccttttga tgtaaaaaat attcaatcat attgtatagg ttcagaggag ataatgagta
     9301 accctctagt taaaaataca aactcagtaa gaatagaagg aactgaccga tttgaaacta
     9361 acaaaaatgt aatagactac ttttttaata gtgcagatgg attctatgta tcagatggat
     9421 accaattagt agatgcaata gcagctgcac cacttactaa aaactctcca atggtactag
     9481 taaatgatgg aagcgataaa actgtattag aaggagctaa gaatataact tctgtaggtg
     9541 aaataaatga gaaggtaata caacagtgta taaatgcttc aaagtcaaat ggacaaccac
     9601 caacaattac agttggaagt acagagatat ataagggtga aaagtttgac actggcaagt
     9661 taaacatagt ggctaaagat aacactggga aggtattacc aatagaagtt gatgggttta
     9721 tagatacaaa tagagtaggt acatacatat tgacattaaa agctacagac gaatggggaa
     9781 aaagtacagg aaaaagagta gaaataaaag tgttggatga taaatcctat gattacaaca
     9841 gcccagaatt taaaaaaatg gtatctactg aaatgtataa cttaatcaat tcctatagaa
     9901 aagaaaaagg caaagagcct ttggtagtat ctagtaggct agaaggtatg gcaaatgcat
     9961 ggtctaagta tatgatggat aaaaaagtat ttgcacatta tatagatgga aaaaatgctc
    10021 cacaagtatt ttctgagttt ggaatgagaa gtgaggaaaa tatagcatat atttatatag
    10081 attctaagaa cgttcaaacg acacaagatg caaaaaactt ggcaaaggct atatttgaag
    10141 tgtggaagaa gtctccagag tacaatgcaa acatgcttag tgatgagttc tatagtactg
    10201 gatttgggct ttatatatta tctgatggtc aagtgcatgc tactcaggag tttttaaatg
    10261 gcaatgaagg tagtttgtaa aaaattccat aaatcttgtt tttttgttaa tttactgata
    10321 ttataaaatg aaaatcacat ctaaagtact ttttgtaatc tgttttgtta ttgctttaca
    10381 ggctatggta ttttaggtgt tttttgtttt agtagtttat agattttgag ggaatcacta
    10441 ctgaggatgt aatttatgat tagctgaatt atataaaaaa attagtagga ggagttcgat
    10501 gaaggtaaat aagagagtat tatcaatagg attgacaata tcattaataa tggcaggagc
    10561 accaaatatc aatgcacttt cgagcataga aaagatacaa gggaaagata gatatgagac
    10621 agctgccaaa attgcacaga aacaaacata tgaaaatgta gtattagtaa atacagataa
    10681 tacactagct gatggattaa gtgcaagtgg attagcagga acagttaaag cacctatatt
    10741 attatcacaa agaaacagta taccatcaga cacagaaaaa atgttaaaag atgtaaagaa
    10801 ggtttatata ataggaactg aagattcaat aggaaaatca gttgaaaatg aattaaagca
    10861 aaaaggaata gaagttaaaa gaataggtgg taatgataga attgaaacaa gttatcttat
    10921 agcaaaagaa atagcttcaa taaaacctat agataaggtt tttataacta atggttatac
    10981 aggtgaagca gatgctatga gtgcgtcatc agtagcatca agagatggat cacctataat
    11041 tttaactaat ggtaaaaatg ttccatttga gaaaaaagaa ggtgtgcaat gttatgctct
    11101 tggttcagag gagataataa gtaatgattt agttaaaaaa actaatgcag ttagattagc
    11161 tggagaagat agatttgaga ctaataaaaa agttataaaa catttttata gttcggcaaa
    11221 agaattttat ctttctaaag gttatcaatt agtagatgca gttgctggtt cttctatagc
    11281 aaaaaatgca cctattgtat tagttgacgg aaatagtgat aagtctgtac ttagaagtgc
    11341 tgataagatt acagcactag gaggaataga tgaaaagaca ctagaacaat gtttaagtgc
    11401 atcatcactg gatgcaagtg caccaacaat aactgttgga aatttaaata tttatcaagg
    11461 tgataagttt gatataagta aactgaatat cgtagcaaag gatagcaatg gtaacgattt
    11521 gacaccagag cttataggaa atattgatac tgataaggtg ggtaaataca aagttactat
    11581 aaaagctact gatattggtg gaaagactac atcaataagt gtagaagtaa atgtacttga
    11641 atataagact aatgatatga atagtagtga gtttaaaaga atggtttcta gtgaaatgta
    11701 tagtttggta aattcatata gaaaagaaaa aggtaaagag ccattacaag tttctaaaaa
    11761 cttagaagga ctttctaatt cctggtctaa gtacatggca gataaaggtc agttttcaca
    11821 tgtaataaat ggtaagaaat ctgttgaagt atttactgga tatggcttga gaagtgaaga
    11881 aaatatagct tttgtaccac ttgttacaaa aagtacttat acaacaaagg atgctagaga
    11941 agtagcaaat gtaatattta cagtttggaa aaagtcagat aaatataatg aaaatatgtt
    12001 aaatagtgat tttgtatata ctgggtttgg attatatata ttgcctaatg gagaagtgta
    12061 tgctacacaa gaatttttaa ataagtaatt taaataagta aaatcaaatt ttttaagtta
    12121 ttaaatattg gagtttaagg gtataaaaac tcatatgctt gggaaaaata ataatttcaa
    12181 aaaaatgact tgcaaaaaga ataaaaatgg attattatag agatgtgaga aatattagga
    12241 atatatggat gattattcta tgtacataat gaagagatgt aattttaata taatgttggg
    12301 aggaatttaa gaaatgaata agaagaatat agcaatagct atgtcaggat taacagtatt
    12361 agcttctgca gcacctgtgt ttgcagcaga agatatgtcg aaagttgaga ctggtgatca
    12421 aggatataca gtagtacaga gcaagtataa gaaagcagtt gaacaattac aaaaagggtt
    12481 attagatgga agtataacag agattaaaat tttctttgag ggaactttag catctactat
    12541 aaaagtagga gctgagctta gtgcagaaga tgcaagtaaa ttattgttta cacaagtaga
    12601 taataaatta gacaatttag gtgatgggga ttatgtagat ttcttaataa gctctccagc
    12661 agagggagat aaagtaacta caagtaaact tgttgcatta aaaaatttaa caggtggaac
    12721 tagtgcaata aaagtagcta caagtagtat tattggtgaa gtcgaaaatg ctggtactcc
    12781 gggagcaaaa aatacagctc caagtagtgc tgcagttatg tctatgtcag atgtatttga
    12841 tacagctttt acagattcaa ctgaaactgc tgtgaaactt actataaaag atgctatgaa
    12901 aactaaaaag tttggtttag ttgatggaac tacttattca acaggtcttc aatttgcaga
    12961 tggaaaaaca gaaaaaattg ttaaattagg agatagtgat actataaatt tagccaaaga
    13021 attaataata acacctgcaa gtgcaaatga tcaagctgcg actattgagt ttgctaaacc
    13081 aacaacacaa tctggaagcc cagtaataac taaacttaga atattgaatg caaaagaaga
    13141 gacaatagat attgatgcta gttctagtaa aacagcacaa gatttagcta aaaaatatgt
    13201 atttaataaa acagatttaa atactcttta cagagtatta aatggggatg aagcagatac
    13261 taatagatta gtagaagaag ttagtggaaa atatcaagtg gttctttatc cagaaggaaa
    13321 aagagttaca actaagagtg ctgcaaaggc ttcaattgct gatgaaaatt caccagttaa
    13381 attaactctt aagtcagata agaagaaaga cttaaaagat tatgtggatg atttaagaac
    13441 atataataat ggatattcaa atgctataga agtagcagga gaagatagaa tagaaactgc
    13501 aatagcatta agtcaaaaat attataactc tgatgatgaa aatgctatat ttagagattc
    13561 agttgataat gtagtattgg ttggaggaaa tgcaatagtt gatggacttg tagcttctcc
    13621 tttagcttct gaaaagaaag ctcctttatt attaacttca aaagataaat tagattcaag
    13681 cgtaaaagct gaaataaaga gagttatgaa tataaagagt acaacaggta taaatacttc
    13741 aaagaaagtt tatttagctg gtggagttaa ttctatatct aaagaagtag aaaatgaatt
    13801 aaaagatatg ggacttaaag ttacaagatt agcaggagat gatagatatg aaacttctct
    13861 aaaaatagct gatgaagtag gtcttgataa tgataaagca tttgtagttg gaggaacagg
    13921 attagcagat gccatgagta tagctccagt tgcatctcaa ttaagaaatg ctaatggtaa
    13981 aatggattta gctgatggtg atgctacacc aatagtagtt gtagatggaa aagctaaaac
    14041 tataaatgat gatgtaaaag atttcttaga tgattcacaa gttgatataa taggtggaga
    14101 aaacagtgta tctaaagatg ttgaaaatgc aatagatgat gctacaggta aatctccaga
    14161 tagatatagt ggagatgata gacaagcaac taatgcaaaa gttataaaag aatcttctta
    14221 ttatcaagat aacttaaata atgataaaaa agtagttaat ttctttgtag ctaaagatgg
    14281 ttctactaaa gaagatcaat tagttgatgc tttagcagca gctccagttg cagcaaactt
    14341 tggtgtaact cttaattctg atggtaagcc agtagataaa gatggtaaag tattaactgg
    14401 ttctgataat gataaaaata aattagtatc tccagcacct atagtattag ctactgattc
    14461 tttatcttca gatcaaagtg tatctataag taaagttctt gataaagata atggagaaaa
    14521 cttagttcaa gttggtaaag gtatagctac ttcagttata aataaattaa aagatttatt
    14581 aagtatgtaa tataaatata ataatataaa aaggcttctt atatgagaag tctttttata
    14641 ttatgttttc gaatacaaaa aaagagacta aatttagtct cttttttatg tgagaaatac
    14701 ttaaatatat aaagatgtaa tttatttaag tatgattatt atactataca ataaaataat
    14761 tgtctactat tctataattt taaggtataa taataattgt atgataaaag aggaggtcaa
    14821 aaaatatgtc agttttagat gcaatcttag ataaagcgga tgaacaagaa attaaaaaat
    14881 taaatttaat agtagataaa atagaatctt tagaaaaaga tatggaaatc aaagagaatg
    14941 aacaattaaa agaaatgaca aatactttta gacttagatt agacaaaggt gaaagtttgg
    15001 atgatatact tccagaagca tttgctgttg ttagagaggt ctcaaaaaga gttttaggaa
    15061 tgcgtcaata taaagttcag ttaattggag gtatagtaat tcatcaagga aagatagctg
    15121 agatgaagac tggtgagggg aaaactttag ttgaggtagc tccagtatat ctaaatgctt
    15181 taacaggaaa aggtgtacat gtaattacag taaatgatta tttggcagaa cgtgataaag
    15241 aacttatgcg tccagtatat gaatttttag gattgactgt aggtgtaatt ttatctaacc
    15301 aagacccaag tataagaaaa gaacaatata aatgtgatat aacttatgga acaaatagtg
    15361 aatttggatt tgactattta agagataata tggtgccaga tttgtctcat aaagtacaaa
    15421 gggaactaaa ctttgccata gtagatgaga tagactcaat attaatagat gaagctagaa
    15481 ctccacttat tattgcagga gatggtgatg aagatttaaa actttatgag ttagcaaata
    15541 actttgtaaa aactgttaaa gaagaagact ttgagatgga tagaaaagat aaaactatag
    15601 cattaacagc aagtggtata agcaaagctg agtcattttt tggtataaca aaccttactg
    15661 atataaagaa tatagaatta tatcatcata taaatcaagc tttaagaggt cataagctta
    15721 tggaaaaaga tgttgactat gttatctcaa atggagaagt aatgatagtt gacgaattta
    15781 caggaagagt aatggatggt agaagatata cagatggact tcaccaagct atagaagcca
    15841 aagaaggtgt tgagataaag aatgaatcta aaactatggc tactgtgact tatcaaaatt
    15901 tcttcagact atatgaaaaa ctttctggta tgactggtac tgcaaagaca gaagaagggg
    15961 aatttgagtc aatctataaa ttaaatgttg tccaaatacc aactaataga ccagttatta
    16021 gagctgattt gcatgataag gtatttaaaa cagaagaaga aaagtatagt gctgttgtag
    16081 aagaaataat aaggatacat aagactagac agccaatact cgtgggaaca gtttctgttg
    16141 aaaaatctga gaagttatct aaaatgctta agaagcaagg tattaagcat caagtcttaa
    16201 atgctaaaca acatgataaa gaggcagagg taatttctaa agctggtaaa ttagatgcta
    16261 taacgattgc tacaaatatg gcaggtagag gtacggatat ttctctaggt gcaggagata
    16321 aagaagaaga agaaaaagtt aaaaatttag gtggtcttta tgttatagga acagaaagac
    16381 acgaatcaag aagaattgac aatcagctta gagggcgttc tggtcgtcaa ggagacccag
    16441 gtacatcaag attttttgta agtcttgaag atgatgtaat aaagctttat ggtggaaaaa
    16501 ctatagagaa acttatgaag agaacaagtt caaaggaaaa tacggctatt gaaagtaaag
    16561 cacttacaag ggctatagaa agagcacaaa aaggtgtaga aggtaagaat tttgaaataa
    16621 gaaaaaatgt tcttaaatat gatgatacta ttaatgagca aagaaaggtt atatataatg
    16681 aaagaaataa agtgttaaat gatgaagata taaaagaaga tattcaaaaa atggttaaag
    16741 acatcataca agaagcagga gaaacttatt taattggaag aaaaagagat tattatggat
    16801 attttaaaca tttatatagt acatttatgc ctgcagatac attactaata cccggtgtag
    16861 ataaaaaaag tgtccaagag ataattgata gcacatatga aatttcaaaa agggtttatg
    16921 acttgaaaaa gatgatgatt ggtattgata aagttgcgga gttagaaaaa acagtacttt
    16981 taaaagtggt tgatcaatac tggatagatc atatagatgc tatggaacag ttaagacagt
    17041 atataggtct taaatcttat gctcaaaaag atccgtttaa agaatatgct ttagaaggat
    17101 atgatatgtt tgaagcttta aataaaaata taagggaagc aacagtacaa tacttatata
    17161 aatttaacta aaaaagttca tgtaattttt attaaatgaa aaataaaata ttttgattct
    17221 ttatattgta attacaatta aataattaag gaatacaaat gaaaaagaag ctgaaaatca
    17281 gcttcttttt ctgtgtgaga aatacggaaa aaagagatgc ttgaaatttc tttcttccat
    17341 ttaatattat actataaaga agatgaaaag tctattagtt ataaactgga ttattaaaaa
    17401 aaattacaat tttgttactt ttttgtatac ataaatatta aaaaaataaa atataagaaa
    17461 aagtttatat cttttggtta attattacaa taagtctcat ttattgaaat aatatcaaat
    17521 atatattata attggtaaat aaggaaaaaa taataaaaat ttgaattttt tagggggaaa
    17581 ataccatgaa taaaaaaaat ctttctgtaa ttatggctgc tgcaatgata agtacatcag
    17641 tagctccagt ttttgctgca gaaactacac aggtaaaaaa agaaacaata actaagaaag
    17701 aagctacaga actagtttcg aaagttagag atttaatgtc tcaaaagtat actggtggtt
    17761 ctcaagttgg acaaccaata tatgaaataa aagttggcga gactttatca aaattaaaaa
    17821 taataactaa tatagatgaa ttagagaaat tagtaaatgc tttgggagaa aataaagaac
    17881 ttattgtaac tataacagat aaagggcata taacaaatag tgcaaatgaa gtagttgcag
    17941 aagcaactga aaaatatgaa aattcagcag acctttccgc tgaggctaat tctataacag
    18001 aaaaagctaa aactgaaact aatggaattt ataaagttgc agatgtaaaa gcttcatatg
    18061 atagtgctaa agataagtta gttataactt taagagataa aacagacaca gtaacttcta
    18121 aaactataga gataggtatt ggtgatgaaa aaattgattt aacagcaaat ccagttgatt
    18181 caacgggaac aaacttagac ccttctacag aaggatttag agtaaataaa atcgttaaac
    18241 taggtgtagc aggagctaaa aatattgatg atgtccaatt agctgaaata actataaaaa
    18301 atagtgacct aaatacagtt tcaccacaag atttatatga tggatataga ttaactgtta
    18361 aaggtaatat ggtagcaaat ggaacatcaa agtcaattag tgatatttca tcaaaagatt
    18421 cagaaacagg aaagtataaa tttactatta agtatactga tgcatctgga aaagcaatag
    18481 agcttactgt agaaagtact aatgaaaaag atttaaaaga tgccaaagct gcattagaag
    18541 gtaattcaaa ggttaaattg atagctggag atgatagata tgcaactgca gtggctatag
    18601 caaaacaaac aaaatatact gacaatatag ttatagttaa ttcaaataaa ctagttgatg
    18661 gattagcagc tacaccactt gctcaatcta aaaaagcacc tatattatta gcatccgata
    18721 atgaaatacc aaaagtaact ttagattata taaaagatat aattaagaaa agcccatcag
    18781 ctaaaatata tatagtaggt ggagaatcag cagtatcaaa tacagctaaa aagcaattag
    18841 aatcagtaac taagaatgtt gaaagactag ctggagatga tagacatatg acttctgtag
    18901 cagtagcaaa agctatgggg tcttttaaag atgcatttgt agtaggtgcg aaaggggagg
    18961 ctgatgctat gagtatagct gccaaagctg ctgaacttaa ggctcctata atagtaaatg
    19021 gctggaatga tctttcagca gacgctatca aattgatgga tggaaaagag attggtatag
    19081 ttggtggttc taacaatgta tctagtcaaa ttgaaaatca acttgctgat gttgataaag
    19141 atagaaaagt tcaaagagtt gaaggagaaa caagacacga tactaatgct aaggttatag
    19201 aaacatatta tggaaaatta gataaactat atatagcaaa agatggatat ggaaataatg
    19261 gtatgctagt agatgcattg gcagcaggac ctctagcagc aggtaaaggt ccaatacttc
    19321 tagctaaagc tgatataaca gactcacaaa ggaatgcact tagtaaaaaa ttaaatcttg
    19381 gtgcagaagt aactcaaata ggtaatggag ttgaattgac agtaatacaa aagatagcta
    19441 aaatactagg ttggtaataa cttatactgt ggagaagatt ccttaattaa tacaggaatc
    19501 tttttctata attatgctat ttattgtaaa ttataacatt ttataaagta ggaggatgta
    19561 gtagtggtag atgcaacttt attttttaca ccacatcaag atgatgaaac cttaagtatg
    19621 ggaagtgcta taattgaaca tgttgaaaaa agtgatacac atgtcatatt atgcacagat
    19681 ggtagtaaat ctataataag aaaagttttg gatgatggag ggtcatgttc ttatcatatt
    19741 aaagatgttc ataaatactc tttatcagaa agtgaatttt caaaagatag agatgaagaa
    19801 tttaaggata gctgtgaagc aatgggtgta aaagaaagta atatacatat agaagataat
    19861 agagcacatg atggtgagtt aagtaaagaa aaggcaaggg aaataatact taagtactta
    19921 gaagaatatc cagatgcaaa agtaaaaact gttactccgt ttaaagctag tggaatacat
    19981 gaagaccata gggcattagg agaagctgca ctagagttat ataaagaagg caaaataaaa
    20041 gatttaagat tttatgtaga accatacgac tataaggatt ttaaaaaagt taatccaaat
    20101 gtagaggtat ggaaggtatt acctagtcaa gaggagaagt tattaagtgc aatgaatgca
    20161 tataaaaaat ggaatcctga aagtggacat tatgctatag gatatcattc agtaaaatct
    20221 cattttgatg agctagctat aaataaaata cagtatgtac atgctccata aaatattatg
    20281 aaagaattag gaggtaagaa ttaatgaaaa tatcaaaaaa gatagtgtct ttgttaacta
    20341 tgacattttt aactgttaca ttatatggaa atacatctaa tgcatctaca aaagatacat
    20401 taacaggttc tggaagatgg gaaacagcaa taaaaataag tcaagcagga tggaaaaagt
    20461 ctgaaaacgc tgtgctagta aatgataatt caatagcaga tgctttatca gctactccat
    20521 ttgctaaagc aaaagatgca ccaatattat taactcaaag taataaattg gatagtagaa
    20581 caaaagcaga attaaaaaga cttggtgtga aaaatgtata tttaataggt ggttcaattg
    20641 cattaagttc agagattgaa aagcagttaa atgcagaaaa tataagtttt gaaagaatat
    20701 ctggaaatag tagatatgat acttctttaa aattagctga aaagctagat agggaaaaat
    20761 ctatatctaa aatagtagta gtaaatggag aaaagggtct tgctgatgca gtgagtgttg
    20821 gagctatagc tgctcaagaa aacatgccaa taatactttc tgattcagag aatggaactg
    20881 aagtagctga taattttata gatagtaaag atatagaaaa atcgtatgta ataggtggta
    20941 catattctat ttctaattct gtagaaagaa gtttaccaaa tgcaacaaga atagcaggta
    21001 gtagtagaag tgagacaaat gcaaaaatta tagaagaatt ttacaaagat actgatataa
    21061 aaaatattta tgtaacaaag gatggtacaa ggagtaagca tgatttaata gattctttag
    21121 ctgtgggtgt attagcttct aaaaatggtt ctccaatact tttagcagga aataagctag
    21181 atagttctca aaaagatgtg ttaaacacta aaattataga taaagttatt caaattggcg
    21241 gattgggtaa tgaaaatgtt gtagaagatg taatagatat gcaagaagaa actaagtata
    21301 ctgttgaaac tattgatgaa cttaatgttg ctataaaaaa atcagatgca aatgacatca
    21361 taagatttaa gccagaaaaa gataaaaata taactaattc ttttaaaata gaaacaaata
    21421 aagctataac tattgaattt gatggtatat ataaacaatc aataactata gatatgccaa
    21481 atggagatat aaaaaacttt ggggacatag ataatgatct agtaataaat aatataaaaa
    21541 atgagacttt agttaacgaa ggaagtatac aagggataga tgtttatgat gagaatggat
    21601 gtaaaataga gaatgaggat tctggagaaa tatggcttat aacgatactt aaaaattcaa
    21661 aagatgtata tattttgaat cgtggagaaa taactaaaat atctaataat tcttctagtg
    21721 taacaataaa aaattcaggt aatattaata ctataagtgg aagtgaagaa ccatcaataa
    21781 gtggtaaaaa acctataact aatgatacag ataaagatga agataaaaag gcacgtggtt
    21841 tatatcctaa agtagaatct tgtatacctg ctttaaaaga ttatgtaaga ataactatat
    21901 caacaaaacc agagagttca aagtataaaa tttattatag agtagttgat gaaaaaccat
    21961 ctgcgaaata tgttggagaa aaaattagta ctaaaggttg gagtatggtt acaatatcaa
    22021 atcaatcttt tattgaaaga gccaaagatg gatcatatat agaagcagta gaaatagata
    22081 cctcaaataa tgaagtttca cgatggggaa aatctagcat tacagatgat ggaatttaga
    22141 cattaaactt attcattttg cagattacta tttagtaatc tgctatttat tttttaaaaa
    22201 ttatcatctt gctaaacaag taatttaaaa ctataacaac tatattagta aatattttta
    22261 caataacatc atttacaaaa agcaaatcaa tcattacata cataattaac atttcaacga
    22321 caccagaaag tagcctaaaa ctaacaaaag acaaaaattc tttaaatacg aaccttattt
    22381 caattctttt actttcaaat acaaaaaact tattagtaac ataagcaaat aaaacagcaa
    22441 gtatccatgc caaagcattt gcaaccataa aattaaataa aataactcta gtaaaaaata
    22501 aatacgaaac tatatttaca agagtagtaa atgctccaaa aaataaatat aatattgtct
    22561 ctctatgctt ttttaaaatc aaatataatc ctcctaatct aatttaatat aaatatactt
    22621 aaagatacta acaaacatta gtatagcaca tctattaata aatcaatata tcttaaaata
    22681 ttataaaaat aaaactataa tattctataa ttaatatgta ctcataatcc atattttatc
    22741 aaaataaaat atgaccttaa tttagaaatc tgatataata aaaattaaat acatataagg
    22801 gggtaaacat gagaaagtac aaatcaaaaa aattgtcaaa gctactggca ttgttgacag
    22861 tttgcttttt aatagtgtca acaatacctg tctcagcaga aaaccataaa actctagatg
    22921 gagtagaaac tgcagagtat tctgaaagtt atcttcaata cctagaagat gtcaaaaatg
    22981 gagacacagc aaaatataat ggagtaatac cttttccaca tgaaatggaa ggtacaacac
    23041 ttcgaaataa gggcagaagt agtcttccat cagcatataa atcaagtgta gcatacaacc
    23101 caatggatct tggtcttact acaccagcaa aaaatcaagg aagtcttaat acatgctggt
    23161 ctttttcagg tatgtcaact ttagaagcat atcttaaatt aaagggatat ggaacgtatg
    23221 acttatcaga agaacattta agatggtggg caactggtgg aaaatatgga tggaacttag
    23281 atgatatgtc aggtagttca aatgtaacag ccataggata tctgacagca tgggcaggtc
    23341 ctaagttaga aaaagatata ccatataatc ttaaatctga ggcacaaggt gcaactaaac
    23401 cttcaaatat ggatactgca cctactcaat ttaatgtaac agatgttgtt cgtcttaata
    23461 aagataagga aactgtaaaa aatgctataa tgcaatatgg ttcagtgaca tcaggttatg
    23521 cacattattc gacatatttt aataaagatg aaacagcata taactgtaca aataagaggg
    23581 ctccattaaa tcacgctgta gcgatagtag gatgggatga taattattca aaagataact
    23641 ttgcatctga tgtaaaacca gaatcaaatg gagcatggtt agtaaaaagc agttggggag
    23701 aatttaattc tatgaaagga ttcttctgga tttcttatga agataaaact ctactaacag
    23761 atacagataa ctatgcaatg aaatcagtat cgaaaccaga tagtgataaa aaaatgtacc
    23821 aacttgaata tgctggtctt agtaagataa tgtcaaataa agtaacagca gcaaatgtat
    23881 ttgattttag cagagattct gaaaaacttg actctgttat gtttgaaaca gactctgtag
    23941 gagcaaaata tgaagtatat tatgcaccag tagtaaatgg agttcctcaa aacaactcaa
    24001 tgacaaaact tgcaagtgga acagtatcat attctggata cataaatgta cctactaatt
    24061 cttacagctt accaaaaggt aaaggagcaa tagtagtagt tatagacaac acagcaaatc
    24121 ctaatagaga aaaatcaact ttagcatatg aaactgatat agatggatac tatctatatg
    24181 aggctaaagc aaacttaggt gaaagctaca tacttcaaaa caataagttt gaagatataa
    24241 atacatatag tgaattttct ccttgtaact ttgttataaa agctataaca aaaacatctt
    24301 ctggacaagc tacttcagga gaatctttaa ctggagcaga tagatatgaa acggcagtta
    24361 aagttagtca aaaaggatgg acttcttcac aaaatgcagt attagtaaat ggagacgcaa
    24421 tagtggatgc tttaacagct acaccattta cagcagcaat cgactctcca attcttttaa
    24481 caggaaaaga taacttagat tcaaaaacta aggcagagtt acaaagatta ggaactaaaa
    24541 aagtttatct aataggtgga gaaaattctt taagcaagaa tgtacaaact caacttagta
    24601 atatgggtat atcagtagaa agaatttcag gtagtgatag atacaagact agtatatctc
    24661 tagctcaaaa gttaaatagt ataaaatctg tttcacaagt tgcagtggca aatggtgtaa
    24721 atggacttgc agatgcaata agtattggtg cagcagctgc tgataacaat atgccaataa
    24781 tacttactaa tgaaaagagt gagttgcaag gtgctgatga gtttttaaat tcatcaaaaa
    24841 taactaagtc ttatataatt ggtggtacag ctactttatc atcaaattta gaaagtaagc
    24901 tttcaaatcc aacaagactt gcaggaagta atagaaatga aactaatgct aagataatag
    24961 ataaattcta ccctagctca gatttgaaat atgcttttgt tgttaaagat ggttcaaaga
    25021 gtcaaggaga cttgatagat ggactagcag taggagcatt aggtgctaaa actgattcac
    25081 cagtagttct agttggaaat aagttagatg aaagtcaaaa aaatgtactt aagtctaaga
    25141 aaatagaaac tcctattaga gttggtggaa atggaaatga aagtgctttt aatgaactaa
    25201 atactctttt aggaaaatag tgaatggaaa gatagtaagg atatacattt tattattaaa
    25261 aaatattcaa tagaagttta taaatataaa ataacaaaaa ggggtgtctc aaaatgaact
    25321 ttttagttca tgaggcactc ttttttgttg ttttatattt ttatcattta aataatgaaa
    25381 aataggcttg tctatatttt tagatagttt tcttattatt gatgaaaaat gtaataattt
    25441 taaactgtaa aatagtgtaa agttatttta taatataaat atagtaagat tttaaacaaa
    25501 ataaggggga ataataatga aagcaccaaa aactatttta acaatactga caatagcgct
    25561 tactttaagt agcatttcta taataccatc atatgcactt acagaggaaa aattaatagg
    25621 taatggtaga tatgaaactg ctgtaaagat aagtcaaaag gcatacagtt ctagttctaa
    25681 tgtagtgtta gttaatgaca attcattagc tgatgcactt tcagctacac catttgcaaa
    25741 ggctaaagga gcaccaatac ttttgacaga aagtgataag ctagatgata ggactgaaaa
    25801 agagataaaa cgtctaggag ctaaagatat ttatttgata ggaggtactg ctgtacttaa
    25861 taaagatata gagaacaagt taaaaggtaa tggacttaat gtagaaagaa ttaatggtaa
    25921 ggatagatat gaaacttcac tgattttggc taataagctt aaggatataa aagatattaa
    25981 ggaagttgct gtagtaaatg gtgaaaaagg attaagtgat gctgttagtg ttggagcacc
    26041 agcagctcaa aataagatgc ctataatttt gtctagtcct aaggacggag tagaagcatt
    26101 tgataaattt ataagagatg agaaagttat aaaagcatac gtaattggag gaacaaattc
    26161 tgtttcaaga gctgtagaaa agagccttcc taatgcagaa agagtgagtg gaaaagatag
    26221 aaatgagact aatgccaaag ttatagaaaa gttttatact gatacaaatt taagtaattt
    26281 atatgtaact aaggatggta gtaaaaatga gaatcaacta atagactcgt tagctgttgg
    26341 agtattggct gcaaaaaatg agtctcctgt tgttttggtt ggtaataaat taaacacgaa
    26401 acaaagggat atacttagta ctaaaaaatt aaatactata acacaggtag gtggaaatgg
    26461 aaatgaagaa gcttttgatg aaattaagag tttacaagaa aaaactgtgt ttgaagcaaa
    26521 aactgttgag gaattaactg atatgataaa tatagcaagt cctaatgata ttataaactt
    26581 taaaccaaaa gagaatactg taaatgaagc ttttagaatg gtaacaaata aaccaataac
    26641 tgtgaatata aagggtgatt gttcaaagac tcttacagta gatatgccta atggtgaagt
    26701 gaataactat gctactttgg taaatgttat agttagaaat attggagaag gtggatttaa
    26761 taaccatgat acaatcacta tattgtcagt tcgtgacaaa aatggtagag ttatagaaaa
    26821 tactagaaat tcagatatag atacgttgat gatattagct tctgctaatg atacaaaatt
    26881 gattaatgat ggatatatag gaaagcttat agataattca tctaatagtg atataactaa
    26941 taatggtact atagataaaa aagttaatca agttgagaat ttagaggcta aagtagattc
    27001 aattgaaaaa gctatagatt ctataagtca aaaagtcaat aaaatacaag atatactaga
    27061 taagattgga tttctaaaga aatttcttag ttaagaatat gtatatttaa gtgaagtgtg
    27121 tgttgtaatt gttctaaagt tatatagtta aacaggttta tatacaaaca gacttataga
    27181 taaatatgta gtttagtata tatttgaata agctttgggg aggtaacatg gggattggaa
    27241 ttgaatgtaa caaaaaaata aataaaagta ttgacaaaac tagaataaag aaaaataatc
    27301 t
//
LOCUS       DAEPOG010000032        26554 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEPOG010000032 DAEPOG010000000
VERSION     DAEPOG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.032"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(190..969)
                     /locus_tag="KR432_001199"
     CDS             complement(190..969)
                     /locus_tag="KR432_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBH0639748.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(962..2389)
                     /locus_tag="KR432_001200"
     CDS             complement(962..2389)
                     /locus_tag="KR432_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0639749.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            2780..3502
                     /locus_tag="KR432_001201"
     CDS             2780..3502
                     /locus_tag="KR432_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639750.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3699..5366
                     /locus_tag="KR432_001202"
     CDS             3699..5366
                     /locus_tag="KR432_001202"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH0639751.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5462..6781)
                     /locus_tag="KR432_001203"
     CDS             complement(5462..6781)
                     /locus_tag="KR432_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0639752.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6822..7568)
                     /locus_tag="KR432_001204"
     CDS             complement(6822..7568)
                     /locus_tag="KR432_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0639753.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7812..9821)
                     /locus_tag="KR432_001205"
     CDS             complement(7812..9821)
                     /locus_tag="KR432_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0639754.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(10259..11692)
                     /locus_tag="KR432_001206"
     CDS             complement(10259..11692)
                     /locus_tag="KR432_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0639755.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11779..13695)
                     /locus_tag="KR432_001207"
     CDS             complement(11779..13695)
                     /locus_tag="KR432_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0639756.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(13769..14629)
                     /locus_tag="KR432_001208"
     CDS             complement(13769..14629)
                     /locus_tag="KR432_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0639757.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(14980..16167)
                     /locus_tag="KR432_001209"
     CDS             complement(14980..16167)
                     /locus_tag="KR432_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0639758.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(16181..17572)
                     /locus_tag="KR432_001210"
     CDS             complement(16181..17572)
                     /locus_tag="KR432_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBH0639759.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(18167..19867)
                     /locus_tag="KR432_001211"
     CDS             complement(18167..19867)
                     /locus_tag="KR432_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0639760.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(19839..21242)
                     /locus_tag="KR432_001212"
     CDS             complement(19839..21242)
                     /locus_tag="KR432_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0639761.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(21506..21997)
                     /locus_tag="KR432_001213"
     CDS             complement(21506..21997)
                     /locus_tag="KR432_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0639762.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(22023..22835)
                     /locus_tag="KR432_001214"
     CDS             complement(22023..22835)
                     /locus_tag="KR432_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0639763.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(22848..23999)
                     /locus_tag="KR432_001215"
     CDS             complement(22848..23999)
                     /locus_tag="KR432_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0639764.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            24269..24910
                     /locus_tag="KR432_001216"
     CDS             24269..24910
                     /locus_tag="KR432_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH0639765.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            24936..26480
                     /locus_tag="KR432_001217"
     CDS             24936..26480
                     /locus_tag="KR432_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0639766.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
BASE COUNT         8769 a         4719 c         2961 g        10105 t
ORIGIN      
        1 ttataattta atatttttaa tttattaatg catactactc tttttattat acataaaaaa
       61 tgtgtacttc ctatattgat agagatgatt caggttttta ttttgataca tctctatcaa
      121 tattttttta gaaaatacac atttttttca aatacttatt tttaattaaa actttaggta
      181 atctattctt tatatataat tattagactt gaaaatatct ttaaaatcaa ccaattcaat
      241 attatttctt tttaaccaca ttttaacttc cttatcacat agcattgcat gttcatatac
      301 tctctcttga ataagagaag atgattttat aagactataa tctataaatc ctggatgagt
      361 aaccatcaat gttatatctt tatctaaatt tatatatttt tcttcaaata atttaacaaa
      421 agatttattt aacttgtaaa aatcattctg aggatattta cttggcatgc taatacatga
      481 atctaaataa tagggacaac ttggtatacc gtattctttt gcagcatcac acattgctga
      541 tattgataca tcttcttcta taccatgtat atcaatatac tcaggcaatt tactcatata
      601 caacttgtaa gtttcaattt gagcacaaac ttcttcatat acttcttcat aattaaaaac
      661 ctctttacct gtcagtagtt gcgttcttct tattttgcta gaaacaaatt ttccaccttc
      721 atctaacaaa ttctttatct taatatgttc tttacaacaa ggttctccta ttactaaatt
      781 aacatgtaaa ccaaaacata catcatcatt ttttaccatt tctatagctt gtcttgaaaa
      841 aggcatattc accatgattc cagcacttgt aacaataccg ttcttataag aagatattat
      901 ccctagtgta acaccttctg aatatcctat atcatctgcc ctaactacta atttcttcat
      961 tttatgcatc tgcctgcttt ctcaactcat caagttgcat ttccatctca tcctttactt
     1021 taacaacatc cgttcctatg atgatttgaa ctgatgtatt actcaattta attacccctt
     1081 tggctcctgt ttttttaata ttatcatcat tcaaaatagt ggcatccttc acttctaaac
     1141 gtaacctcgt catacaatta ttaagagtca ctatattttc ataaccacca caatttttaa
     1201 ttatctgctt agccatatat gaaaaattac catgagaaag ttttaaattc ttttcttttt
     1261 cactaacttt atcacctatc tctatctcaa cttcatgtcc tggtgtttgt acattatctt
     1321 ttattattat atatttaaat gttacataat ataatacaaa gaatacaact cctacaggta
     1381 taactaacca cgctttatct ccataactaa aacttaagac atagtcaatt atacttgctc
     1441 cattaaatga acccaagcga gcacccagta aatatagtac aactccacct agaccagaat
     1501 aaaaagaatg aattatatat aactttggag atgcaaataa aaacgaaaat tctattggtt
     1561 cagttactgt tgccataaaa gaagtcaatg ctccagcggt cattgcacct tttacttctt
     1621 gcttcttttt atcttgtgct gttttatata tagcataaga aatagcaggt attgaaaaca
     1681 tcataattat gtaaaatcct gtccagaaga atcctgcatt tgggtctcct gccataaatc
     1741 taggaatttc tcctcttata acttctcctg ttgatgttgt atattcaccc atttcaaata
     1801 atatgtaagc atttaaaact tgatgtaacc cagttggtat tagtgcacgg ttacagaata
     1861 aaaacaagaa tattcctatt gcaccagctc ctcccaacca aaaaccaaat tgattcattg
     1921 cagcttgtgg atatatccat aataaactaa atatatatga taaaattaaa gcaaccactg
     1981 gtgatagtgt aattacacat ttttctcctg caaagaagct taaaaatgct ggaactttaa
     2041 ctttatatgt tttattgtaa gtccatgcta tacaagctcc aacaattatc ccaccaaata
     2101 cacctgcatt taatgtatca ttcaataact ctaaatttga cttaaatatc atatatgcaa
     2161 ctacggctgc aaccattaca cttgtcttgt ctttagactt tgaataagca ctaactgctc
     2221 ctaataaaaa taagtagtcc acatttgaaa atactatatt agatatatta gatatagctg
     2281 gcacattaag catatttgga ccaaataata atcctaatat acctgctgct ggcattacag
     2341 ctattggcac cagcatagtc tttcctaaaa gtgacagttg ttgcatcatt ttgtttaaga
     2401 acctcttcat atccaatccc ctttctattt cggtttaatt ttttgtgaaa acgatttatt
     2461 cacattttat attttaaata ttatatttgt ttaaacaaat tgtcaatatt ttttattcat
     2521 tttattttta tcatttagtt tcatatctaa atccttttaa gtatattgct atatctagcc
     2581 tattttttaa atttattttt tttattttta aaatatgttt aaccatgtat ttgaaacaac
     2641 ccacaaaata gttcaaattt atccttttta tggtttatat aattctattt tcactataca
     2701 tgtctaaaca catttgatat aatttgttca tatcagttca ataatattac taaagacctt
     2761 taaggaggga taggggtata tggctaaaaa taaattttca gagatatatg aagtattgaa
     2821 agaggaaata cttgatggta aatatacaag taatatgatg ctgcctactg aacttcaact
     2881 tattgaaagg ttttcatgta gcagaaacac tgtacgtaga gcaatttctc aattaaacac
     2941 agaaggatat gttcaaagta taaaaggcaa gggtgttgta gtgttagaaa atagttgttc
     3001 taatgatttt tttctaaata tgcacaattt caaaggtgta gaatcgattg tagaagataa
     3061 aaaggttaat actgctacta gtgttctcca cttcagcaaa atcttaattg ataataagtt
     3121 atctaaaaag acaggtttta aagttggtag tgaagtatat taccttcaca gactaagata
     3181 tattgataac atacctaaaa ttctcgacat taactatttt ctatgtagta tagttaaaga
     3241 ccttgatgtt tctatagcac aaggctctat ttataaatat atagaagagt gtataggtac
     3301 aaaaattgtt tcatccagaa agatatttaa aattgaaaaa gctacagagc ttgagttaaa
     3361 aacgctacct ctaaatgatt ataattgtgt tggtgttatt aaaaatagtg tatatactga
     3421 tgatggaaag ctctttgaat atacagaatc aaagcataca cctgaaacat ttgtattcat
     3481 ggatgttact caaagatatt aaaaaaatta tgtttaatta aacataattt ttttacgttt
     3541 agacacttta tcccatacta acctcactat tctatataca attatatgtt tatacaaata
     3601 attatttatt gtatatttta gctttaatat tagtttaata cttgacaatt tgtttaaaca
     3661 tattataatt aacttataaa ttacaggagg tatcaattat gaaaaattgg tggaaaaaag
     3721 caactgtcta tcaaatatat ccaaaaagtt tcaaagatag caacaatgat ggtataggtg
     3781 atataaatgg tattatagaa aaattagatt atctgtattc tttaggtgtt gatttattat
     3841 ggttgactcc tatgtacgtt tctcctcaaa gagataatgg atatgatata gaagattatt
     3901 ataatataga cccaaaatat gggacaatga gtgactttga aaaactctta aaagaagctc
     3961 ataaaagaga cataaaaata atgatggata tggtcctaaa ccatacatct actgaacata
     4021 aatggtttaa agaatctaaa aaaagcaaag acaatccata tcgtgattat tatttctgga
     4081 aagatgctaa acctgatggt tctgttccta ataactggat atccagattt agtggaactg
     4141 cttggaaata tgatgaaact actaaccaat attatttaca tttatttgaa gaaactcaag
     4201 ctgatttaaa tttggaaaat gaaaaggttc gtgaagaatg ttacaaagta ctagaatttt
     4261 gggctgataa aggtattgat ggttttcgtt tagatgtagt caacctactc tccaaaacac
     4321 ctggattacc tgatgaccct ataacagggc caaaaggaga tggaagaact cattacgctg
     4381 atggtcctcg tatacacgaa tatttacata atatgaatca aaaagttttc aaacctaaaa
     4441 atatagttac tgtaggtgaa atgtcatcta ctacacctga agagtgtatc aactatacta
     4501 gagaaaatcg agaagaacta tctatggttt ttagcttcca tcatatgaaa acagattata
     4561 aaggaggcgc aaaatggact aatgaaatgt tctctctgga taaattaaaa aaagcacaat
     4621 ctgactttca atataaaatg tatgaaggaa agggatggaa tgctctattt tactctaatc
     4681 acgaccaacc tagagcttta tctcgatttg gagatgatag attttttcgt caagaaagtg
     4741 ctaagatgtt agccataaca ctatttggtc tacaaggtac tacatacata tatcaaggtg
     4801 aagaaatagg tatgacaaat gcctacttta ctaaaataga tgaatatgat gatgctgaaa
     4861 gtaagaatgc atactatcat atgattaaat ctggtattga tgaaaaggag gcattgctta
     4921 tacttcaaca aaaatccaga gataatgccc gtactcctat gcaatgggat aatactattt
     4981 ataaggggtt ttctaatcat aaaccgtggt taaaagttaa taatgacaat attagtgtag
     5041 aaaatgagct taatgattct cgttcagtat tttatactta tcaaagatta atcaaatatc
     5101 gtaaacaata tgatattttt acagaaggta cttatagatt gttagatgaa aatcataaaa
     5161 atctatttgt ttatgaaaga gagtataaaa accaaaatct tcttgttata agtaatttta
     5221 ctcatgataa tgtagtatat aaattacctg aaacttattt gaataaatta aactacacaa
     5281 tacttgaaac caattacact agagatataa ttgaaaataa aatggaaata aaaccttacg
     5341 aaagtataat gatatattat aaataaagtt atttatataa aaattggtat aacaccaggg
     5401 tgttatacca attttactaa attcaaaata acatttatag aatatctata gattacaata
     5461 attatttgac ttctacatca ttatatttct ttgaatatac tttagtcata tagaaatata
     5521 taggaactac aattatcatc ccacctaatc cccaaactat cttcataggt tcatcaatac
     5581 ctgcttttat cagtaataat atacttataa taactgcaaa tattggtata attgctcctc
     5641 caggtattct aaaatttaca tctttatcag catacttctt tctcaatatt agtacagcta
     5701 aacatgtagg aatatattga ataaatctag atacaacact tatttgagat aatgttgtga
     5761 atgaacccga ccatgctatt agtaaagttc ctatgacaga tactataatt gctatataag
     5821 gagctccatt tttattagtt tttcttatag acttaggcat taatccttgt tcaaccaaag
     5881 cagcagcaga ccttggtgta aatatggatg atgcaatatt tatacctcca acagatacta
     5941 tagttcctat tgtaattata tacccaccta taggacctag catcttaatg gcagcatctg
     6001 caacaggagt agctgaccct gccaagccat tacccaacac ccctatactt attcccagta
     6061 taagcatata tattactgat actacaaaca ttaccattac caatgcttta ggtacatttt
     6121 tcttagggtt ctccatatct tttgctgcaa cagctaaaga ttcaaatcct gtaaaagcat
     6181 aaaataaagt tatagatact gctacaaaag ctgatgacgc actttgaccc ttttgtatta
     6241 ttacaaatgg ggtaaaatta gaacccttta taaagaataa tcctacagct ataaataaaa
     6301 ctattggtaa taattttcct atagtcatga tattatttaa gtgcttagat tgtttcattc
     6361 ccataaaatt tataatccca agtcctacaa ctaatatacc aattattata tttttaataa
     6421 gtggttctgc tgcttgtggc catacagatg atagtgttac agcaaaaaaa tttgccatag
     6481 tagcccaagc aatcatacta actatccact tcataaatcc aacttcaaat ccaacaaaat
     6541 ctccaaatgc ctcttttgca taaagataag gccctccatc agtatcaaac caactacctg
     6601 cttcagcaaa acataaagca atagaaacag ctaaaagcat acaaaaaact aatactaata
     6661 tacttgctgg acctatatct gtataagcat caccaggtaa cccgaatata cctgacccta
     6721 ttattgcatt gattcctaat agtacaatac tccaaaaacc taacttaatg cctttttcca
     6781 ttgttattcc ccccaaagcc aacttaatat atttatattt tttatttaga tgcttcttta
     6841 actattgcca taaatatttt tttcatatta tcataatctc tagtcatcat ttctggatgc
     6901 cattgtatcc ctattacaaa ctcacttcct tccttttcta tagcttcaat taaaccgtct
     6961 ttagatgtag ctgaaacaat atatcctggt gctactttgt tgacaactaa gtgatgaaaa
     7021 ctattaacta aaacttcttt ttctcctaat atttcatata attttgtccc ctcctttatc
     7081 tgtactgtat gtgtaggtac attagataga tgttgctgat tgtgtttaat ataagcacct
     7141 tcaattaaag ataaatcttg atacaaacta cctccttcag ttacatttat aatttgttct
     7201 cctctacata tacctaaaac tggttttttc atatctaaag catgttttaa taatttcata
     7261 tcaaaactat ctctctttgg tgatattgcc cccaacttat tatgaggttc ttctttccat
     7321 atgagtggat ttacatcttg tcccccagat agtataagcg catctatatt agatacctgc
     7381 tcctttataa tctcatcatc atatacaatc ggtacaatat atggtatagc tttacacata
     7441 actactgatt gtatataatc attatttaca taagcccttt catagccagg aaacattcct
     7501 ccttcatcaa ttagtaaatt acctgatatt cctattattg gatatttctt tttaaccaat
     7561 ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc tacttctctt aaagagcaag
     7621 tagcatacca agattttatt tatttgaaca ttataattca ttttagaaat actttttttg
     7681 aatacaagta atataaataa ctttatttac ttatatttta ttttaatcct gtttagtttg
     7741 acttttttaa attgttttaa gtacaactaa taggtaaaat aggcatttag attttccttt
     7801 ttatcaacct attaaatact gtttaatata ttttttcccc ttctatttaa ttcacttccc
     7861 tttctaccag attcacatat tatcaaatcc agctctttta acatattaag tattcccctt
     7921 acctcacctt ctgttatact gtagcctaat tcatttattg aattaacaat accatttcta
     7981 cctatatttt tattaagatt attggcattt tttaaacact ccattactaa ctttactttc
     8041 tctatagatg ttctatcctt taatatgctt atttctttac taaaatcttc agttatattt
     8101 aataagttat gaggtaaatc atttattaaa ataacatcct cagaactcat aatgttaata
     8161 tacatagcag tattttttaa ttctcttata ttacctggcc attcatatgc attaataatg
     8221 tcttgaacac cttgactaat tgctctcttc cttttcataa aatgctttaa cataacttct
     8281 atatcttctt ttcgctctct taaaggtggt atatttatgg gcaatacatt tatcctatag
     8341 tataaatctg ctctaaatct atctcctttt atcatttcta ataaattttt atttgtagcc
     8401 gcaattactc taacatctat atctattaca ttttctcctc caaccctcat tacttgattc
     8461 tcttgtaata ctctaagtaa tttgctttgt aagtatatag gcatgtctcc tatttcatcc
     8521 aaaaatatag ttccattgtt agcctgttca aataatcctt ttttacctcc ttttaaagct
     8581 cctgtaaaag caccttcttc atatccaaaa agttcacttt caagtaaatt ttctggcata
     8641 gctgcacagt ttatcgctat aaatgactga ctatgtcttg gtgatgcatt atgaatagac
     8701 tgagccataa gttcttttcc tgttccactt tctcccgtta taagaattgt ataatcagat
     8761 tcagctattt tcatacctaa tttcttagtt ctttccatct tcgttgaaac tgtttttata
     8821 tcatcaaaag tatatctagc aacttgtccc ttttctctaa gcttctttga taggttttgc
     8881 tccaattttc ttatataagt tatttcttga atacaaaaat aatacccagt aacccttcca
     8941 aaactctcta tattatactt atttacgttt aagtacttat tattaaattt tataacttca
     9001 tcatgtgcct tactcaaact aaaaatcttt ttaatattct cattctctaa ttcatctata
     9061 tttttcccta taatattact ttgaatatct agtatatccc ttaaactatt attgcaaata
     9121 attatgtttc ccttatctga taccattgcc atacctaagt ttgataaatt tattatagaa
     9181 tttaattcat ctgcttttat agtaagttct ttgtatttag tatttattcc tgaatataaa
     9241 ctaattattt catctgtata cttaattagc ctctcagtaa tttctttatc atccagtttg
     9301 agttttgcta ttatttgaat aaacgttgaa ctatcaatat acctatggcc taaatcaata
     9361 acttcttcta tatattttgg aactaaactt gactctcctg gcgttatagc tatcttaata
     9421 tttctatatt cttcattggg attgtatggt ataaaattca aatggtttat tcctatattg
     9481 tataaattag atattgtttc caatacagta tgcttattat cgtttactac taatacatct
     9541 atttcttctg gcaatgaaaa taacttatat atatcctttt gctttatgct cctatttata
     9601 gttattatct ttgagacatc ttctacatat ttttttattt ttaatactct atcatctatc
     9661 attaccaata ttatgtcatc ttttattaat tccttctctg ttattttatt taaataataa
     9721 ttttttatca ttactttatc tttaaatatc atttctagat ttttatttaa gaaacattct
     9781 aatttagaca acccatcagt tactactgct attgttttca tttgattact ccttttaaaa
     9841 tatactctac tcttttataa tatacatctg attttcactt taatagcaca aaaaattgta
     9901 catttagtat actaacatat tcctttatta gttttatgtc cctaaatatc atagttgcaa
     9961 tatctatcat atttcttaaa cttctataaa gcaaaaattg tgccaaatta ataattttct
    10021 taattttatt ctaaagttcc tcaatagtat ttacagactt agcacttata gcttataagt
    10081 ctttatatta ttaatctatt tttataggtt aattgtacaa atcatatatc acttttacct
    10141 attaaatgca ttataaaaaa gatggaataa cgattcgtca ctccaccttt tttgtaataa
    10201 aaaattgtat atataaacac tatttactac tcttctaagt acaaacagta tctacatact
    10261 ataacctttc accattagat tctattactt gtttatacca gtcaaaactc tttttcttaa
    10321 tcctagacaa gtctccactt ccatcatcat acttatccac gtatataaac ccatatctct
    10381 tggtcatctc accagtagat gcacttacta aatctataca tccccatgga gtatatccaa
    10441 ataaatcaac accatcttct acagcttcct tcatttgctc tatatgtgct tttaaatact
    10501 ctatacgata gtcatcattt atcactccat tagattcttt tttatcataa gctcctaaac
    10561 cattttcaac taccatcata ggtattctat atctatcata aatttcattt agtgcatatc
    10621 taagcccttc agggtctatt tgccatcccc attcactaga cttaagatat ggatttttca
    10681 ctccacctaa tatgttgcca cttgtattat cttctttaga atcactactt acacatgtag
    10741 acatataata actaaatgta taaaaatcta cacaaccttc ttttaaaatt tctaaatctc
    10801 ctttttccat ttttatttca atattatttt ctccaaaata tctattcata taagttggat
    10861 attcgccacg cacttgtaca tctccacaga aataattcat tactctacta tctctttgag
    10921 ttttaactgc atcatctgga ttacaagtat aaggatacat tgttgcaaat atttgcatac
    10981 atccaacttg ataatttgaa tctatttcat gagctaattt aacagcctta gcacttgcta
    11041 caaattgatg atgtaaccct tgaaaacgaa gttgaggtat atctacttgg tttataaaat
    11101 ctgtagtccc ttcatttaat attccttgtg ataagtagcc tcccataggc attgtagctg
    11161 aatttatttc attaaatgtc agccaatatt ttacttttcc tttatatctt ttaaatattg
    11221 tttcacagta attcatatac aagtctatta cctctcttga aacccatcca ttgtatttct
    11281 ctgttattgc aaatggtatt tcatagtgag atattgttat taatggttct atattatatt
    11341 ttaaacattc atctatcaca ttttcataaa atttaagtcc tgcttcattt ggttctttct
    11401 ccattccagt tggaaatatt ctagtccatg ctattgaaaa tctaaatacc ttaaatccca
    11461 tttctgcaaa taaagctata tcttctttgt atctgtgata aaaatctatt gcttcatgac
    11521 ttggatagaa tgttcctttt tctataactc tcgttattct tttacttact ttttgagaac
    11581 ccccagtaca catatctgaa caagaaggac ctttcccatc taaattccat gctccttcac
    11641 attgatttgc tgctgttgct ccaccccata aaaaatcttt tctaagtacc attactctac
    11701 ctccaataat ttatttatag taaatttatt ttaaaataaa aatacatata tctaattttt
    11761 aattaataaa actctctttt aagctaaaac agttatcaat tcctgactat tatttacatg
    11821 cttttgtgta ttttctataa tatccacata atcagaataa ttagtaacaa ctactggtgt
    11881 ttctaagcaa tatccttctt ttgatatgct ttctatatca aaagttatta atgtctgacc
    11941 ttttttaatc ttatcccctt gattaacaaa agatttgaaa tgtttacctt ccaactgaat
    12001 agtatttaac cctatatgta tcaatagttc acaaccatta tcagatataa ttcctattgc
    12061 atgttttgtt ggaaataaag tcattatagt cccatcaaat ggtgccacta cttttccttc
    12121 tttaggtgtt attgctacac cttttcccaa tatacctgtt gaaaacgctg catcttgtat
    12181 ctgctccaaa gcctttactt caccttttat tgggctatat actacttctc ttttgttttt
    12241 ttctacaacg ccttgcccat tattatcttt agaaatttct actatagaat catctttcca
    12301 aaaaaataat gttaaaacaa atcctactac aatagctact attattgcta ttatagttgc
    12361 ataaacacct ttcatatctc cagttgttgg atttatataa ttaggtatac caaatattcc
    12421 aagtccaccc attacataac tttttacatt cattatcccc attacagctc ctgccacagc
    12481 accacctaca caactatata tgaatggtgt ttttcttggt aaagttattc catatattgc
    12541 aggctctatt acaccacata tccctgaaat aactgcaggt atacacagct cttttatctt
    12601 cttatctttt aatttaaagt acattgctgc tactactgat gtttgtgcaa aagatggtac
    12661 aaatgctccc actaatattg tgtcaaaccc catagtcata aggtttgaca tagctattgg
    12721 tactaatccc caatgtagac caaatattac aagcacttgc cacaataaac cagttaatac
    12781 accacataaa attggtgaaa agttaaataa agctaaaaat ccatttccaa ctatattaga
    12841 agcataggta gctataggcc ctataaccat aaatcccaat ggtaaagaaa ctaataaaac
    12901 tgccattggt acaaagaaat tctttactac atctggtatt attttagcaa acactttctc
    12961 caacttagct gctaataata ctattataat tactggtact actgttgaag tgtagttcat
    13021 tgctattact ggtaatccta agaaagttat atatactggt gattcaaata ttgttccagt
    13081 aaatagattg tataaaggtt gacctataga tgctagagta tctaattgaa tacctgaata
    13141 gcataaaact gcacctataa gcattccaat aaatggtttt aatttgaatt tcttagcagc
    13201 tgtatatcca agaattattg gcatatactg gaacattgca tctccgattg catttaacat
    13261 aatatatgca ccatcagttt ctccatacaa acccaatgct gtaaatactg cattaaatcc
    13321 ttttatcata ccagcagcac acagaactcc taatattggt tgaaaaactc ctgatattat
    13381 gtctataaac ttattaagta atccttttgg cgcatctgaa tctgaattat ctattgtatc
    13441 tcctcctaat ccagccaaat cgcacacaac ttcataaaca tctggaacat gattaccaat
    13501 aactacttgg tattgacctg cacttttcat tacagtaact ataccatcca ttttttttag
    13561 cacatcatca tttgctattg attcatcttt taatttaaaa cgtagcctcg ttatacagtg
    13621 agataaactg ctaatattat ccttccctcc tacattctca atgatttctt ttgatagttg
    13681 ttcatatctt ctcatttttt ctcctccatt ttctttttga aatatgaagg tttggccaac
    13741 ttaatgtcac cacccaatat atataaacct aattattagg atttatatct gacttaatac
    13801 ttttacttac cactctttca acgtgaatag ttaagtataa tttttcctca tctgttaaat
    13861 cgtagttgta ttttttatgt ataaattcat ccaacttttc tatgcattta taagcatttt
    13921 tatatttaac ttttattaca tttagtaaat catcttcatt attttcatgt aaattattac
    13981 taacaattcg ttctgcaaaa tactttaagt gagtaataaa tctatgataa taaactgaat
    14041 cttcatcaaa tactattctg taatggtatt taaccatatt tgaaatttct tgtattattt
    14101 gtgtaatctc atatatagtt tttatacttt gatccatttg tgcattaact atatgcagtg
    14161 ctataaatcc agcttcatct tctgatagtc taatttcaaa ttttttttct atataatcca
    14221 atgcttctaa ccctatttta aattcactct tataaaatct ttttatatcc caaagtaata
    14281 cattttttac acttactcct tcacgagctc tttctattgc agtatacata tggtctgtta
    14341 aagaaatata aatactatca tttaatcttt tccctagttt ttctttagca tataaaatta
    14401 tttcatctga taattccata tatcttatag gaatatctgc tattagttct ttaaatttat
    14461 cagatatatt tgggtctgaa agtttgaata tcttatcaat tttatccttt gatatatgct
    14521 ctcctgtacg ttttttataa gctaaacctc tccccataac tactatttcc ttatgatttt
    14581 catcagttgt aataattaca ttattattga gtattttttc aataatcatt gtgatgcccc
    14641 ctttattagc attaagttaa aataaatctt ttttaattat ttagaatatt taaaatgtaa
    14701 tttattacat aaaaaaaaac ctatttatta cacattaaat agactcccat gagtcctgag
    14761 taataaatag gtttagcttg tatttaaaca atcactatcc gtttgtattt tattttaatt
    14821 attatttatt atatgttagc aaatttattt tttagtgtca actttttctc aatattttaa
    14881 ttctaaataa attatataaa ttgcatctgc aaagtactat ttttataaat aaacaaaata
    14941 aaaaaggtat tacttgcttt aagtaatacc catttatttt taatttaagt ggtttttctt
    15001 aatctcatca agtagtccat cttctaaata aatttctaat gcacatccta ccattgcctt
    15061 tactgctttg tgaagtttat cataagcttc ctcagaattt gcataattta gaaattcttt
    15121 ttcatgaaca tataccattg gacttatatc taaggctatt tctgtataca tagtagggca
    15181 aacttgactt acattaccta tatctgtaga acctactgga ccatctttac cttctaatat
    15241 atctgttata cctgactcaa tcaaattatt ctttgttatc ttttgaagta ctttgttatt
    15301 tattatgttg tcaaaagaat tttcaaagta tctataagaa agcttagctc ctgtcattaa
    15361 ctctgcacct tttgcacagt ttataacttt tttagtcact tcatctaaat aacttctttc
    15421 agcagctctt acaaaaaact tacaagctgc tttttctgga actatatttg gagcctctcc
    15481 tccatttgaa actattccat gtattctaac atctgatttt acatgttgtc ttagtgcatt
    15541 tatcccagca aaagtaagtt gtactgcatc tagtgcatta actcccatat gagggtgact
    15601 agctgcatgt gctgataccc cttcaaaact aaactctaat gaatctattg ccaaagcctt
    15661 acagtttata ttattaaaag cttctatatg catttgataa cttacatcaa catcatcaaa
    15721 agctcctgcc ttaactaaat cacattttcc tcctgttgtt tcttcagctg gtgttcctat
    15781 tagtacaatt tttcctttaa aattttcttt taacttactt aatactatac aagctcctag
    15841 tgtagatgct gcaatccagt tatgcccaca agcatgagct ggttctttat tgtctccata
    15901 tccaggaagt gcatcatact cagctaaaaa tgctatggta ggtccatcat tatctcctaa
    15961 ttcagctcta aaagcagtct ccatattaca atatggataa actgtatcaa atccatattc
    16021 cttcattttc tctactaagt attttgatga tatgtattct tcttccccta attctggatt
    16081 gttaaatata aaattacaaa cttcttcaat ttcttttcca agttcattct ctatctcata
    16141 agctctcttc tttatactct ctatcataac ttacctccca ctagaatcct agatataatc
    16201 ctattgtcaa aaataccatt acaagaattg ttaatactac cattaaagga attacgaatc
    16261 taacccactt tgaccaagga atattggcta ctgctagtgc tcccattaaa acacctgatg
    16321 taggtgttat taagttagtt acacctatac caaattgata agctgtaact atcatttgtc
    16381 ttgatacatc tactaagtcg ccaagtggtg caagtacagg tattgtaagt gatgcaagcc
    16441 ctgatgctga tggtattaaa aatgctatta atccttctat taagaaagtt aaatttataa
    16501 atatagtttt tggtaatcca tttaataaat ttgctgcaga atttaaaatt gtatctatta
    16561 taaatccatt ttctgctatt attacgattc cacgtgcaaa tgctatacat aaccctgctg
    16621 taattaaatc tcctgctcca cttataaatg aattaactat ctcatcttgt ttaagccctg
    16681 atgctattcc tgatattata cctatagctg taaatatcat tgcaatttca gttatgtacc
    16741 actctagtct taaaactcca tatatcataa tcgccatacc tattgtgaaa atggctatta
    16801 ccatggcttc tctttttgta aattccttta tatttgtatc actattaacc attgattctt
    16861 gatttagtga tatatcgtgt actaaagatt ttttagggtc ttttttaact ttattggcat
    16921 acatcataac aaatcctata gatatagcca tatatattat aaacataaca actctaaact
    16981 ctattccaga acctggtact atttgtgata atgcttgtgc tattcctaca gagaaaggat
    17041 tcgtagtagc tgctccaaat ccagctgttg ctcctataaa tactgtggcc actgctgtca
    17101 atgaatcata tcccatactg gtcataagtg gaataaatat catataaaaa ggtagtgtct
    17161 cttcactcat tccaaacgtc gtcccaccta gtccaaatag aatcattgaa atcggtatta
    17221 ttaaaaattg aaacttcttc attttattta cagtatgagc tataacagct tctatagccc
    17281 ctgttttatt aactatccca aaagctcctc caactattaa aacaaatcca actacttcag
    17341 ctgcatctat aaatccgttt ataggtgctg taaatacatc agtaattcct tgtggatttg
    17401 atggtgcttt ttcataagtt ccagctacaa caactgacct tccgtctata tctactcttt
    17461 caaaatttcc acttggcact atccaagtaa gaattgccat taacactatt agcgaaaata
    17521 ttattgtaag tgtctgtggc atagtaaact ttttcttttt taaactgctc atttttaatc
    17581 tccccacttt ttaaattaaa tattttatta ttgcatccct ttatcctatt ttgtatagat
    17641 gtaaaaaagc tcaacatgga tacatgttga gctaaatgtg ctaaatacaa catacctgca
    17701 tcttcccttt atcttgttta agatagctct ccatcaaaat tcgggataat tttgatgaca
    17761 gtactgtacc tattaaatac agtcccagca ctagctttgg acactagcac ttcggcactt
    17821 actcctttta tattgcctca ttggtttcca acctcttcaa tattaatctt agtaaatgca
    17881 acctctacct caatctgttc gttagatgag gtaattctta ttaattttta ttgattcaaa
    17941 gtaattgtag cataatatgt cgagattgta aatattttca gtataataat tcttacttta
    18001 tgatgtcatt ccatttttca caaataattt tttctccata taatttaaat actgtagtat
    18061 attttaaata cacacaaact ttccacaaat ttttaattat tataatacct ttataataaa
    18121 aaataaaagc catgtaaact tatcaattta cacagcttta tatacattaa tctactccaa
    18181 gtacagcttt aagctttgat gctattgtaa taactcgtgg accaaatata acttgaactg
    18241 agtttttctt tttaacaact ccacttgcac ccaattcatt tacccagaaa ccatcttcat
    18301 agaatccatc aattgaattt agctctaccc taagtctagt tgcacagttt gtaacatttt
    18361 ttatattatt acttcctcct aaaccttcta ttatagaatg tacaagcttt gtatctttat
    18421 catctacttc ctcttctact gcattgttgt atgctgtttt accagcatct acagattgtc
    18481 tttctctata ttctttctta ctataaagct taacttcttc tgaacttctt cctggtgttt
    18541 ttatatcaaa cttcttaatt gcccatgtaa atattatata agtaactata aaaaatattg
    18601 gtatcaaaaa taataatggt aacgcatcca ctttttgtgg ttgtaataaa ttaggaaaca
    18661 tatttctaaa tgattctccc attatagaaa cattaaacat ttctgtaaat acatacgtca
    18721 atcctgccag tggtacatat gctacaaaat acaataatgg tgcaacaaat aggaatgtat
    18781 actctattgg ttccgttacc cctaccaaca tggctgttac tattgctggt atcaatatac
    18841 ttattgcttt tttcttatta gcattatcag ccaacttata catagctaaa gctgctgctg
    18901 gtaatattgc aaaatgtatt aataaacgac ctgttgtgaa gttacgagtt atataagata
    18961 gtgtatctgg gtctcccatt tgagctaatc ttatattatt aacaccctcg taaattgctc
    19021 cacctacttc cattgttcca cctactcttg tatattctat agggaatgca attaagtgat
    19081 gtataccaaa tggtaataat gctttatcca ttgctccaaa agtaaatgtt cctaataatc
    19141 cactagaacc tatccaatta ccaagtttag ataatgccaa ccctactggt ggccatataa
    19201 agtacattgc tgtacccaat actattgatg ctataaacat cataatcatt gaaaatcttg
    19261 gacctgagaa aaaagataat gcactaggaa gttctatgtc aacaaatttg ttatgtactg
    19321 cactagctac aaatccagct attatagctc caaatatact acaatcatat gaaaagattc
    19381 ctaagaaaga accatataaa gaacttcgac tatatgcttc tgcctctcct aatccagatg
    19441 ccataaacgc ttctgctgta actgtatctg aatttatacc atttgatgct aacaatgtac
    19501 ttataactgt gtgcattcct ataaataaaa caattcctcc taatgcaccc catccttttt
    19561 cctttttaga taatccaaaa gctatacctg taacaaagaa tattggtaag tatctcatta
    19621 ccataaatcc taaatcgttt attactttaa agaatacatt tataaatgac ccttctttta
    19681 ttagtccgct aagccctaat gcagaaacgt tttccatatt tgcaaaagct gaccctaatc
    19741 ctataaatac tcctgctaca acaagtaata taacaggtat catcattgct ccagaaagta
    19801 cttgtaattt atccttcata gtattatttc ctccattttt attgaaatgc caggctatat
    19861 aatgcatata tatatacttc tattgctttg tataatgtat ctatacttat attttcattt
    19921 gcctgatgtg aatttggatt atctccctct attgtgggtc caaatgcaac tccatttttt
    19981 aacactcttg cataagatgc agctcctttt gtaaaaagtt cggcttcttt tccaaaacct
    20041 tgtttatatg cacttgataa tttatttatt aattcactat ctggttttac atacagtaat
    20101 tttaaatcct tatatatatc aactattaaa ttttcttttt tcccaatttc attaattcta
    20161 ttagttataa actcccaact tataccctta ggatatctaa agtctatttt catattcaat
    20221 tcatttttct ctaagaagat actcattata catattgtag aaactcccat atctacatct
    20281 tctttatata tccctaattt ttttccatga ttatcatctg taaagtatga atttaaaata
    20341 tcccttatta taacgccttt atcatttagc tttttaattt tctctaaatc ttttgctaaa
    20401 ctaaatgcac agttatatga cctgtgtgga tttctcgata aagccctttc tcctgtatat
    20461 cttactaata ctttaccttc ttctagttct tctatctctg tataataagc taatgattct
    20521 actaaatctt tcttatcatc agttttaaaa acagcctcaa ttttgtcaca tacaaatcca
    20581 tcttctttgt ttgactttat atcaactaaa ttgtgatttc taaatttatc tttatctatt
    20641 ttctttctta gattgaagta taaaatacca ttttctccat ttactattgg gaagtttcca
    20701 tctggagaaa atgcaaattt tggctgttca ttgacactaa agtaatgctc catacattcc
    20761 caagtcgtct cttcagctcc acccactatc aatctaatct ttctcttcgg cttttcatta
    20821 agttctctta agaaaagtaa tgcatataaa atccctatca aaggtccttt gttatcatta
    20881 actcctctac cataaagata gttatctttt tgacatactt taaaaggttt actcttccaa
    20941 agttctttat tatatattgg caccacatct atatgattaa gcacccctac tacttcttct
    21001 ccttctccat attctatatg aattgcatat ccatcaaagt cttttacaac aaaatctttg
    21061 tcttttgcta tttgaataag cttatcgaag gcatttctta tttctatccc aaatggttga
    21121 tttatatctg ttgtactttc atctctaatt gaaggtattt caactaaact tctaatatcc
    21181 tttatgatat tttcttgatt ggattttaaa taatcaataa ttagttcttt gtttactatc
    21241 atcttaatta tttatcaccc ctttatatta attttacaaa gcatttcagt tcatatctac
    21301 attataaatt ttgaatatta ttttttcaat atttcaaata cttctctaaa ttaccataat
    21361 gatacggtaa acaatcaaca gattatgaca aaatcatact atacctatta ctaatatttt
    21421 aacttatcta ttacgtcttt tttaatgtaa ttttattcca aaaatagata taaaaagatg
    21481 tagccttagc tacatctttt tatatctatt caacttccat tattatgcta ttaatatcta
    21541 catttccagg ttctatccta tcttttactg ttctcttaac agaatcagca tcatccatta
    21601 taactacagg agttattata tttaaatcat ttgacgttat aaaatctaaa tctacttcca
    21661 ttattaaatc tttagctttt accttatctc ctgcctttac cttaacatca aacggctttc
    21721 cttctagtga cattgtctct aaacctacat gtattaatat atttgtatta tcattactct
    21781 taatcattat tgcatgcttt gtatcaaata cattgactat ttcaccatca acaggagaat
    21841 ataacttacc ttcatttggt attattgcta ctccatctcc catcatcttt tgggcaaaaa
    21901 ctgcatctgg aacatcttct aagttcacta aatttccctt aaaagggcta tatattttct
    21961 caatcttatt ttcttttttc ttaaatatat taaacataac acactctcca tattataaaa
    22021 ttttatgata atatttttct taaagctttt gctacaccat catttacatt agtatcagtt
    22081 acaaaatctg ccatagattt tacttcatct gtagcatttc ccatagcaat accaagacct
    22141 gcaaatttaa tcatggatat atcattttca ttatcaccta tacaaataac ttctttgcta
    22201 tctataccta atatatcaca aaatttttta actgcaatac cttttgaagt acctttactc
    22261 ataacttcaa aatttatctt tccagaactt actacttcta aatcttcttg ttttttaaat
    22321 tcttcctttg cttctctcaa agaatctacg ttttctgaga aaagtataca ttttaaaatt
    22381 tcattatcag ctttttctaa taaagtttca caacttgcat tttctattat ttcaattctg
    22441 tcttcttctt tatttttact atttattaat ctatatggat gttcttcacc tatttcattg
    22501 ttggatataa ctattttaca gcctttaaaa tgtttgttta aattatactt ttcacctatt
    22561 gtatagattt tctttatagc ttctttgctc aaaaccttct tatactcata tcccattaat
    22621 ttaaaatatg tcccattagt tgatataata tatatatcat cccctaaaac acttgtaaaa
    22681 tatttcatag cattataagg tcttcccgta actaaggcta ccttgatacc cttttccata
    22741 gcttctttta gtgctttaat attttcttct gatacttcaa atcctttccc catcaaagta
    22801 ccatccatat cagtacaaat taatttatat cgcatttgta tcacatccta gtaagatttt
    22861 tctaatattt caacaaggtc ttctttcttt ccaaaaggta aaaatcctaa aactggacag
    22921 cttgttattt gttctaattg ttcctttgtt acattaaact cagacatttt tgtcttaagt
    22981 cccactctct ttaaaaaact ttctatttca tttcctaaag ttttagctgc ttcatcatct
    23041 tccacttttt ctaattcttg gtttagcatt ctcgctactt tggcaaattt ctctatatta
    23101 tccttataga acttttctgc aaattttggg aacactatag ccaaagcctc accatgagga
    23161 atatgtgtaa ctcctcctat tatttcactt agtggatgtg gtgctgctgc tccagcattt
    23221 gccagtgaac ttcctcctaa tgtatctgct actgctaact ttgttctgta ttctatatta
    23281 tttttatctt ccattacttt aggtagatag ttaatgacta attccataga tttttcagac
    23341 attatctctg taaagtcatt agcatttgga tttatataac tttcaaatgc atgagtaaaa
    23401 gcatcaaatg ctgttgatgc agtaattctc tctggcagag ttaacataag ctctgggtca
    23461 actatacatt cttttgagaa acaattttga tgaaatattg tatttttctc gcttcctctc
    23521 gatattactg ctgcttgtgt aacttggctt cctgttccag aagtagttga tacagctatt
    23581 aatggtaatt ctttttcact taccttatcg tatattttaa atggatttgt atatgtacta
    23641 aacatataat cccaatctat tttttctaaa ccaaaagtca atgctaatac ttttgcagta
    23701 tctatacttg aacctcctcc aacagctaaa acaacatcta cagattcttt tttagctaat
    23761 tcgaaccctt catttattaa ttctacagtt gggtttggct ctactctatc aaagtgtaca
    23821 acttctatac ctttttctat taaattcaat gtaactcttt tatataactt gtctaatggc
    23881 tctacatttg gagtagtaac taacaaacat ttctttccat attttcctat tattctaggt
    23941 agttcactta aagttccttg tccaaaattt attcttgatg gttgataaaa gttaatcata
    24001 tttttcctcc cttaaattat tgacttcata ttcaaattat acatatcata ggatttttaa
    24061 ggcaatacct tcactgttaa ctcttattta ctaaagctta tggcaattat tagacaataa
    24121 atgttttatt attaccatat cattatgtaa attttattaa gtacccatgt tataatttta
    24181 taatcaatct tattgtataa ttgacttaat aataaaaata gagtatatac acttttttat
    24241 gacaaatagc ttaaaggaga attaaattat gaaatatttg atagacagtg ctaatcttga
    24301 tgaaattcaa aagtgtttaa agtatggatt taagggaata acagctaatc catctatgta
    24361 tcttaaagag aaggtaaact tccatgaatt tattgagaca tgtactaatt taaatccaga
    24421 aatattaact gctgaagtca ttggaaataa tttatacgaa ttaattaatt catgtgataa
    24481 gatattaaat ataaataaaa acactataat aaaaataaat ttctctgaag ttggcttaga
    24541 acttataaac atattaaata gtaaaggtgt aaaaactgca tgtaccttga ttttcaatgt
    24601 aaatcaagca tcattagcta taaatgcagg tgctgattac ctattccctt ttataggtag
    24661 aaatgatgaa aatgggaatg atggtataaa aaaccttaca gaaatatgta accttatatc
    24721 aataaatgga tataatacta aagttgttgc agcttctatt aaaaatgtat tccacttaac
    24781 tcaagcatct ctttgtggat gtgattatgc agctgtaaca tatgatttat ttaaaaaagc
    24841 ttcttttaac cagcttacag tagatggtgc caatactttt gaaagagatt ggtccaaggt
    24901 taataaatag taactaagat ttaggagggt ttgcattgaa caaaagatta ttagatattg
    24961 ctgaattatt actcaacagc tcagactata ttactgtaga tactattgcc gaagaattaa
    25021 aagttagtaa taaaactatt cgtaatgacc ttgttatttt agatgaatgg cttttagagt
    25081 ttaatttatc cctagataaa aaaactggtt caggggttat tattctaggt aatgaagata
    25141 taaaacttaa agttattaga gatatcaatg ataaatcaaa ttgtatatat gcatactctc
    25201 ctgaagatag aaaaaggtat attctaagta aattatttat taaaaattct aagtttagga
    25261 ttcgtgatat ttgcaatgaa ctacatgtta gtagagctac tgttcataaa gacttggtag
    25321 ttattcaaaa ttttttgcaa aactttaaag tgactctagt tagaaaaacc aataatggag
    25381 tatatttaga gggaaaagag aaagatattc gaaaagcagt ttttgaactt gctactgtta
    25441 ataaaagtta tactgaactt aaagaaatat tattctcgaa ctctaatgaa tgcaaagata
    25501 ccccatcaac aaaaatattt aaggagctat ttaactatga ttttgagaaa ttatctcaaa
    25561 tcactctaca caccttacaa gtagaaaaaa gtaaccttag tgatgagtat tatataaact
    25621 tcttaataca tatagctata tgtattaaga gaatttcaat taaaagatat attaacttat
    25681 ctgaaggttt ttttagagaa ttattagaac atgaatactt tgaaaaatca aaaattttat
    25741 gtgctaacct aagtgaagct tttgacgtag aatttattga tgaagaggtc tgttatatac
    25801 ttcttcatat agaaggatta cttaaatcaa taaagaaaac tactactctt gaacctctta
    25861 aaaatgaaga aattacagag tataatctat ctgaagcaat agctgttcat tggggaagta
    25921 tactaaattt aaacttaaag gatgatttaa tattaataaa gtctcttgct aatcacctta
    25981 aatcagttct acacagaata aactatggat ttactattac aaatcccata ttaaatgata
    26041 taaagagaac atttccttat acttataagg ctgctaaaga gtctaatact gttatcaaac
    26101 agttgatgaa ttatgaagtc aatgatgacg aaataggata tcttgcttta tatttaatat
    26161 cagcaataga tagaagcaaa tctcccctaa atactatttt aatatgtcac tgtagtgatg
    26221 gtgtatctaa tttattagtc caaaagttaa gttttgaatt taatcagatt aatatagtta
    26281 aatctatacc tctatcttct atttcattta cgaactttga tgatgtggac ttaatactaa
    26341 ccactgctcc tgtagacttt gaacataatg ctgaactgat aaatataaat gctcttttaa
    26401 gtaagaatga tatttccaga cttagtacaa ttataaaaaa actatattct gaaaaaaata
    26461 aaatattatc ttataaataa acaaaaatag gcttgtctca acatagagat aaacctattt
    26521 ttcattgtcc aaatgataaa aatatatttg attt
//
LOCUS       DAEPOG010000033        26383 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEPOG010000033 DAEPOG010000000
VERSION     DAEPOG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.033"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(187..942)
                     /locus_tag="KR432_001218"
     CDS             complement(187..942)
                     /locus_tag="KR432_001218"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0639767.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="KR432_001219"
     CDS             1383..2372
                     /locus_tag="KR432_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBH0639768.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KR432_001220"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="KR432_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBH0639769.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="KR432_001221"
     CDS             complement(3583..4332)
                     /locus_tag="KR432_001221"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0639770.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="KR432_001222"
     CDS             complement(4399..5286)
                     /locus_tag="KR432_001222"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0639771.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="KR432_001223"
     CDS             complement(5403..6404)
                     /locus_tag="KR432_001223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH0639772.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="KR432_001224"
     CDS             complement(6829..7710)
                     /locus_tag="KR432_001224"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0639773.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8033..9226
                     /locus_tag="KR432_001225"
     CDS             8033..9226
                     /locus_tag="KR432_001225"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH0639774.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9432..10550
                     /locus_tag="KR432_001226"
     CDS             9432..10550
                     /locus_tag="KR432_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBH0639775.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10681..10926
                     /locus_tag="KR432_001227"
     CDS             10681..10926
                     /locus_tag="KR432_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBH0639776.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10913..11659
                     /locus_tag="KR432_001228"
     CDS             10913..11659
                     /locus_tag="KR432_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0639777.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11944..12819
                     /gene="hslO"
                     /locus_tag="KR432_001229"
     CDS             11944..12819
                     /gene="hslO"
                     /locus_tag="KR432_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH0639778.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13210..14010
                     /locus_tag="KR432_001230"
     CDS             13210..14010
                     /locus_tag="KR432_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0639779.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14537..16777
                     /locus_tag="KR432_001231"
     CDS             14537..16777
                     /locus_tag="KR432_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0639780.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17191..18726
                     /locus_tag="KR432_001232"
     CDS             17191..18726
                     /locus_tag="KR432_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0639781.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            18742..19503
                     /locus_tag="KR432_001233"
     CDS             18742..19503
                     /locus_tag="KR432_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639782.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            19841..20709
                     /locus_tag="KR432_001234"
                     /pseudo
     CDS             19841..20709
                     /locus_tag="KR432_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20903..21154
                     /locus_tag="KR432_001235"
     CDS             20903..21154
                     /locus_tag="KR432_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBH0639783.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21266..22045
                     /locus_tag="KR432_001236"
     CDS             21266..22045
                     /locus_tag="KR432_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0639784.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22180..22830)
                     /locus_tag="KR432_001237"
     CDS             complement(22180..22830)
                     /locus_tag="KR432_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639785.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(22827..23330)
                     /locus_tag="KR432_001238"
     CDS             complement(22827..23330)
                     /locus_tag="KR432_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0639786.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23379..23894)
                     /locus_tag="KR432_001239"
     CDS             complement(23379..23894)
                     /locus_tag="KR432_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0639787.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24210..24701
                     /locus_tag="KR432_001240"
     CDS             24210..24701
                     /locus_tag="KR432_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0639788.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            24685..26088
                     /locus_tag="KR432_001241"
     CDS             24685..26088
                     /locus_tag="KR432_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0639789.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         9804 a         3236 c         4359 g         8984 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtta
     7801 atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa attttaaata
     7861 aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa
     7921 aaataaattt tatgagaatt taataactaa taattaatat atttaggtta taataaatat
     7981 gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac
     8041 tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca
     8101 aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt
     8161 atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac
     8221 tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc
     8281 aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt
     8341 atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag
     8401 tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac
     8461 tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat
     8521 aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg
     8581 caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag
     8641 cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa
     8701 tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc
     8761 aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg
     8821 aataataggt aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca
     8881 aggtgaagtt ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg
     8941 aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac
     9001 ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc
     9061 tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc
     9121 cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa
     9181 agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc
     9241 aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat
     9301 ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat
     9361 gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg
     9421 gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt
     9481 cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag
     9541 attatcttga taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg
     9601 taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc
     9661 atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg
     9721 gattaattga gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat
     9781 tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta
     9841 gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt
     9901 atgtaggtac aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag
     9961 tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt
    10021 caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg
    10081 cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata
    10141 taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta
    10201 gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata
    10261 ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg
    10321 ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt
    10381 attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag
    10441 gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg
    10501 cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat
    10561 tataaagatt aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct
    10621 tatatatttg tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt
    10681 ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg
    10741 acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt
    10801 ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt
    10861 acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca
    10921 atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg
    10981 ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga
    11041 attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc
    11101 tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg
    11161 agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt
    11221 ggactgtgtg ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga
    11281 aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat
    11341 aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga
    11401 agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga
    11461 acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca
    11521 acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga
    11581 tataaaatat tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt
    11641 ctttgtatgt aaaaaataag atatttttga ctatataaga taattattaa aaataaccat
    11701 gatataaaaa tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg
    11761 acttttattt tctaataatt ataaagttct atagataaaa attaaaatat aataactatt
    11821 gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata
    11881 gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata
    11941 ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc
    12001 gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca
    12061 acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat
    12121 gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc
    12181 acatcagatt ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa
    12241 gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa
    12301 gttataaaag acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga
    12361 gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta
    12421 gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa
    12481 ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta
    12541 ccttctataa ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt
    12601 ttggatggtt tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt
    12661 tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata
    12721 atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat
    12781 agtgaagaag agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg
    12841 atataagtaa tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat
    12901 ttattaataa tagacatatt tatattaaaa aataaattta ataaattttt aagcttaata
    12961 aattttaaga attaagaagc tttctggctg taatatttgc aatgttgcag ttagaaggct
    13021 tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata
    13081 atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg
    13141 aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa
    13201 attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg
    13261 atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat
    13321 agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta
    13381 tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg
    13441 gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaac
    13501 ttaactagga agcaaaagga agagcttaga aataatgagt ttaaatcagc tttgagagag
    13561 cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca
    13621 cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg
    13681 acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa
    13741 gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt tgttaagcca
    13801 aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat
    13861 gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa
    13921 atgtatggta taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct
    13981 gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata
    14041 agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt
    14101 taaagataga ggtttttaat gtttaaaatt ttaaagttaa gttgaataat tgtatagaat
    14161 tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata
    14221 ggcaagatac tttttttatg taactaatac cattttgagg ttattaactt aagaatttta
    14281 tataatttaa cactcatata taatgatgat atttaggtag aatagatgca attacgctta
    14341 aataaaataa ctgtattttt tataaaaatc aaaccctaca ctatactacc agataaaatc
    14401 tacatgctat aatttgacct gtgtttataa aaaaaggagt aatctaataa aatagtattt
    14461 cacttatgta taactaacaa ctattattac gattttctct aaataattta aagaaaaata
    14521 atgcaggagg taaaatatga gcaatgaatt tataatttta aaaggttgca aagagaataa
    14581 tttgaacaat atttccttaa aaataccaaa aagaaaaata accatattca caggggtgtc
    14641 gggttctgga aagtcatcta ttgtatttga gacaattgca aaagaatcac aacgccaact
    14701 aaatgaaagg tttagtacat ttgtaagaag ttttttacct aagtatggag aggttaaagc
    14761 tgattgtata gaaaatctgt caacaccaat tatcatagac caaagtagac ttggtggaaa
    14821 ttcaaggtca accctaggta ctattacaga tgtaaattcc tttttaagag ctttatattc
    14881 aaggtttggt agtgagtata taggtaatgc taatatgttt tcttttaatg atattgatgg
    14941 tatgtgccca cactgtcatg ggcttggaaa aaagctagtt cctaatatga accaaattct
    15001 tgatatgaat aaatcattaa atgaaggtgc tatattgctt tcagggtttg gagtgggttc
    15061 ttggcattgg aatatattcg ctgaatcagg attttttgat attaataaga ggatatgtga
    15121 ttattcagaa gtagaattgg aaaagttttt gcatggtaaa gcagaaaaga ttaagataga
    15181 aaatgctggt caaactaaca tgacatatga gggattaatg gttaaattta atcgtctata
    15241 cttaagtaga gatggagaaa tatctgaggc tactaagaaa aaattgagta aacttcttat
    15301 agaagataga tgtcctatat gtgatggaag aagacttaat gaaggagtat ataggtcttt
    15361 aattaatggt tataatatag cagatttaac tagtatgcaa attgatgaat tggctgaagt
    15421 tattaaaagt ataaatgagc cagaagcaca acctttaatt aaaggaataa tagaaaagtt
    15481 aaacagtatc atagatatag gtcttggata tttaacactg gatagagaga catcttctct
    15541 atcaggaggg gaatctcagc gtataaagat ggttaagtat ttaaatagta atcttgttga
    15601 tttaatgtat atttttgatg aaccaagtgt aggacttcat ccaaaagatg tgtataaatt
    15661 aaataatctt ttaaaaaaat tgcgtgataa agggaataca attatagttg ttgaacatga
    15721 cccagatgtt attaagattg cagaccatgt tgtagatgta ggaccaaagg caggtaagta
    15781 tggtggaaaa gttgtgtatg aaggcagtta taaaaatcta cttacaagtg gtactttgac
    15841 agggaatgct ttaagtaaat ttcttcctat taaagaaagt gtaagagaac atagtggata
    15901 tttagaaatt aaaaattgta ataaaaacaa tttaaaaaat gtatctatta agatacctaa
    15961 aggtgtattg actctagtta ctggagtagc aggagcaggt aaaagtacgt tgattaagga
    16021 tgaattttta aagcaaaatc ctagtgctgt acttatagac caaagccctg tttcagcaaa
    16081 ttcaaggtcg agtttggcaa catatagtgg aattatgaac aacataagaa gcatattttc
    16141 aaaaacaaat ggcgtaaatg cttctttatt cagttcaaat tcagaaggag cttgtgataa
    16201 ctgtaagggt tctggaatag tagaaatgaa tcttgctttt atggagagta taaagagtac
    16261 ctgcaatgtt tgtgaaggta aaaaatttaa aaaagaagtt ttagaatata ggtttcaaag
    16321 gaagaatata attgaagttc ttgaaatgtc tgttttagac gcaattgagt tttttaattt
    16381 aaaacagatt aaaacgaaat tacaatgtat tgaaaaaatg ggtattggat accttacttt
    16441 aggacaaact cttgatactt tgtctggagg agagtgtcaa agattaaagc ttgctagtga
    16501 attaaataag gaaagttcag tgtatatatt agatgaacca acaacaggtc ttcatatggc
    16561 tgatattgag aattttataa gtataattga agagattgta gataatggaa atacggtcat
    16621 aataattgaa cacaatatag atgtaattaa aagagctgat tggattattg agctgggacc
    16681 tgaaggaggg acaaaaggcg ggagagtaat atttgaaggt atcccaaaac aattatgtaa
    16741 caataaaata tcattaacag caaaatatat agtataataa ataaaatata ataaaattat
    16801 aatgatattt taataaatat aatgggtata taaaatgata attaaactta acagtaataa
    16861 ataatgtgag aaatattcaa atttaattaa ataaagtgtc agaaatttgt ttaaatttta
    16921 gtattttaac gattctgacc ttttttctta tttttatgga gagtatcgaa gatggagtct
    16981 ctaatagtta cttttttgtg tctacttgca agtttgatta aaaaatggct atacacactt
    17041 gtgacttcag tcatgggagg ttcagattat aatcttaata tataatttgt gaaataattt
    17101 gatgacgatt tgctgatgta taaacaataa agattaacta cattaaaatt taaatcttgt
    17161 taagtaaaac ttataaaagg agagagatgg atgataaatg atattctaac cttattatct
    17221 gaaagaagtg atttttttat aaagctatcc atagaacatt tgatgatttc tattatttct
    17281 ataataattg cgattgtttt gggtggtata attggtatta ttgtaagtga atttcataat
    17341 tcttctaaac ctatattggg aactatcaat tttctataca ccattccatc tatttctatg
    17401 ctaggatttc tgataccatt ttccggaatt ggaaatacta ctgctgttat tgcacttacc
    17461 atttatgcat tattgccaat ggttagaaat acatatactg gaattactaa tgtggataaa
    17521 aatattcttg aagcagctag gggaatgggc agtacagatt ttcagatatt gtataaaata
    17581 aaacttccat tagccctacc tgttattatg tctggtatta gaaatatggt gattatgaca
    17641 attgcattag ctggtatcgc ttcatttata ggtgctggag gcctaggtgt agccatatat
    17701 agaggaatca cgacaaataa cacagcaatg acaattagtg gaagtatact tattgctcta
    17761 ttagctttaa tatttgattt tattttaggg ctggtagaaa aattttttca acgaaaaaga
    17821 aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa gccttattct tatagcaact
    17881 atctttacgt cttatttatt taatccttca aagactcaaa ctattcatat tgctactaaa
    17941 cctatgactg aacaatatat tcttggtgag atgttggata ttttaattga aaaaaacaca
    18001 ggtctaaacg tggaaattac acaaggcata ggagggggaa catctaatat ccagccagct
    18061 atggagaatg gagaatttga catttatcca gagtatacag gtacaggctg gaatatggta
    18121 cttaagaaaa atgaattata tactgaaagt atgtttagta aactacagtc ggaatacaaa
    18181 actaaactta aaatgcaatg gactggtatg tatggttttt ctaatagcta tgcattagca
    18241 gttagaaaag aagttgcaga taaatataat ttaaaaaata tatcagactt aaaaaatgta
    18301 tcttctaaat tgacatttgg agctgaatat gacttttatg agagagagga tggatatact
    18361 ccattgtgta atacatatgg actgaacttt aaagatacag ttgatttaga tattggatta
    18421 aaataccaag ctatcaatga aggtaagatt gacgtaatgc ctatatttac aactgatggt
    18481 cagtatagtg tttcagatgt tatagttcta aaagatgata aaaatttcta cccatcttat
    18541 caatgtggaa atgttgttcg tttggaagta ttagaggaaa atccagaact atcagaggta
    18601 tttaagatgg ttggagatat tttgactgat aaagaaatgg ctaaaatgaa ttatgacgta
    18661 gaaaacaaca atttagaacc tagagaagtt gcttgtgagt ttctgaataa gaaaggtctt
    18721 ttataaattg gaggaaatat aatgacacct attatacaat ttaaaaatat taaaaaacaa
    18781 tataatgata aaactataat agataatctt aatttagata tagaaaaagg agagtttttg
    18841 actgtaattg gttcgtctgg ttctggtaag acaacactct taaaaatgat taatggtctg
    18901 attttgccag atggaggaaa tattttgata aaccaaacag atatcaaaaa cgaggactta
    18961 attaaattaa gaagaagaat aggatattgt gtacaaggaa gtgttctttt tcctcatatg
    19021 actgttgaag aaaatatttc atatgtacca aatctcctaa gtaaaaagaa taaattagaa
    19081 gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat tgccaaatga tatgaaagtg
    19141 cgttacccat cagaattgtc aggtggtcag caacaacgtg tagggattgc aagagcttta
    19201 gcttcttctc cagaaatttt attaatggat gaaccttttg gtgcagttga tgagattaca
    19261 cgaaagcaat tacaaaaaga gataaaagaa attcataaaa aaacaggagt tacgattatc
    19321 tttattacgc atgatattta tgaagctttg attttgggaa caaagacttt ggtattaaat
    19381 catggagttg ttcaacagta tgatactcct gagaatattt taaatactcc tgctaaccaa
    19441 tttgtagacc agttattgaa tataagaaaa tctataatag atgatgataa tttaaaagaa
    19501 taaatttaaa tttaaaatca aattgttgtg ataaaaacta ttgatttaga gctaacttca
    19561 agttgtatac ttgaagtgga aaagttagat taaggtatag aagagaattt agcagttggc
    19621 tgtagacgag gtaactgctg aaggtgcttt caataaatgg ctagaacctg tatctattga
    19681 agattataat aaattgaaat aagaagctca atatacagag tagataggca cttcaaatag
    19741 aagtgtcttt tgtgtgccat aaaataaatc aaaatattag caaagaatgt gaaaaacaaa
    19801 ataaaataat atgctatctt taaaatagga ggggtgctat atggataaca atttgattac
    19861 cattgaaaaa actttagatt atatagaaaa acatctttta gatgagatgt tagatttaaa
    19921 tgtaatttct aaagagatag gctattcaaa gtaccattta catagaatgt ttacatcagt
    19981 tgtcggattt agtatacata attatataca aagacgtaga ctaactgaag cagcaagaat
    20041 gctcattttt acagaaaaac caattattga aattgcttta atctcggggt atgacacaca
    20101 acgttctttt tcaacagggt ttaaaaaaat gtttaagcat agccctaagt tttttagaaa
    20161 acatcaagat tttttaccac tacaattaaa atttgatatt aataatagaa aaaggttaca
    20221 tggaggtatg gttttagatg ttaaaaaagt taattgtaca gatataaata ttgttggcta
    20281 ttctggtaaa gtaaaaaaag gatttcatgt tattggaaaa tgttggaggg atttgcataa
    20341 aaataaagag ttgatagaaa acaaggtaga cttggatttt ttaattggag ttaatgatta
    20401 tagtaatttt aaagaaattg aaaataatcc tacatttaca tatattgcag gtgttcaagt
    20461 agctactttt ggtaaattcc taatggaatg gaaaaattcg tattaccgaa aagtaattat
    20521 attgttttta cttttattgg gaaaaatgag gatagtatgc aaaatgttgt agaatatatc
    20581 tatcaagaat ggttccttaa ttcaagttgc cagtttaatg aaaataattt atatgatttt
    20641 gtaaaatacg gagaaaaaac tgataagaat ggtttgagtg aaattcagtt ttgggttcca
    20701 atcatttaaa agaagggatt atgtataaag taggtggatt tatataggca aagttttata
    20761 aaagtatgta tttataaata atttatagtc tgtaggaaag aaatagtttt actataagat
    20821 tatgataaaa tacacaatac tatgataaaa tagtactaaa taatagattt aattttgcaa
    20881 aggataaagt gttttaataa aaatgaatgt agaaaacgaa ttacttaaaa acctagatag
    20941 attgcataca acagagttag gcgttgtaag aattaagaaa aatctttctt tggagacaaa
    21001 tgatgtagtg aactggtgta aaactaaaat agaatctcca aatgccatta ttaatagaaa
    21061 gggaaaaaac tggtatataa gtgtatatga ttgtattata acagtaaacg ctcacagtta
    21121 tacaattatt acagctcata aagaaaagaa atgaattgaa aaatacaaag gtcaaaagca
    21181 ttttccactg tttgtagtac ctatgaagaa gtatataaaa taatattact tttgaataat
    21241 aatttaaatt ttggaggttg aaaaaatgaa taatactgta aaagaaaaat tggcagagtc
    21301 actaacttct gatactactg atattttacc ttatttgcct tatttattac aagatttttg
    21361 ggaattagga agttcaccag aacaaatgtt acaattattt agaaagcata taccttttaa
    21421 tgaggataca aaagttttgg atttagcttg tggaaaaggt gctgtatctg tgaagcttgc
    21481 aaaagagttg aatatacata tcaaaggtat tgatttgata cctgaattta ttgattatgc
    21541 gagaagaaag gctattgaat ataaggttga tgctttgtgt gattttgaaa tgaatgatgc
    21601 taatagttct gtaaaaattg agcacgatta tgattgtgtg atttttggag ctgtagggga
    21661 tattttaggt aattataaag aaactataag taagctatta cctacaataa aaagtaaggg
    21721 gttcatttta attgatgatg catatgtagt agataaatct acaaatgaac agcttaattt
    21781 tgagagggtg tatcccactt acaatgagtg gtgtatattg ttcaaagaat taggtctgaa
    21841 actaattgaa tgtataccat gtttagaact tgaaaatgat gttgaagagg aaatgaactg
    21901 gattataact cgtgcaaatg agctaattaa aaaatatcct tctcaaaaag atatgtttga
    21961 acaatatgtt attagccaaa aaaatgaata tactgattta cagcaggacc ttgttggagc
    22021 aacttggatt ttacaaaaag aataagataa atattagact agataatcaa tgttgtagtg
    22081 aattatccca tgtctagtga atataaataa cataaaaaac cgtcaagatg aatagtttat
    22141 tcgtgcttga cggtttttag tatttgttga agtaaaattt tatttcttta gtatatcact
    22201 tttagtcaat ccatttgatt tagcttgagt attccattga tgattcccca taagtttata
    22261 taggaatcta gggaagttag gtgaaataaa aatagtactt ttgttggaat catctatatg
    22321 tatattatca ctaagtatat tttttgaaag ttcttttaat gcaattccta aatctttttt
    22381 aggtccttta cccaaaggta cggattctag tgaaccaaac atctccccag ctccaattcc
    22441 aataccttga acccatgtaa agttgatttt tttgcaccaa tgatctatca tttgaattgc
    22501 caaatgatta tgttcacctt cattgaatcc acagttaact attgcataca ctggaatagg
    22561 ttttttattt tgtgttttta aaaatttttc acatttctct aaaaaatgaa gtaaatgaga
    22621 tggaatacca tctacataaa gaggaaatgc tattattaat ctatcacaat taagtatggc
    22681 attaagagcg tcaggatact tagatgtgct atctacttta aatgtaaaaa ctttattatc
    22741 attttgaata agttgtttta attcagataa aaaatactgt gaattatttt tatttacttt
    22801 tggactacca tttatcatta ctatattcat atggattcac ctcttatatc ctgtatattt
    22861 tcatagaaac aaacagagtg ttcttttata ttgaaattta tagcatttcc atttactaat
    22921 ttcgtagcag tttctttttc agcttctgta atacaatctc catagaatat agcttttaaa
    22981 tttatctgtt tatcatatct gcttttatga cgcatcatat tatcttttat tacaaagtat
    23041 ggaagaatat aattaatgct tttatcaagt acgtttttga caaaagggct aaaccctccg
    23101 taacagcatc tgcttattat aataattcca tcggattcat ttataagttt acccatgttg
    23161 ctgtaataat cttttacaac acattcagaa ggagttttaa tccagcaacc aaaacgacca
    23221 atacatttgt ggatttcttt tgtgtttgga agtattttta tattgtcatc aagcttgggt
    23281 aataactttt ctaattcttt gtcagttaaa tcatgtatta aaagcttcat aaagtccatc
    23341 tccttttatt atgtatgttt atttctaaaa attatatctt atagattttt cagtaaatct
    23401 ttacaccaat taataacaaa attataatag tcaactccat aaatcattgt ggcatacata
    23461 tatgtatatt ggtcaataca atgattttct tctacatcaa ttttttgttt acttaattta
    23521 tttagaagta attctacttc agttattaac atttttaagt ttgtttcaat tatttctttg
    23581 ggtagatact tgtaaaagaa tatattaaca aggtggtctt gctttgtttt agaaaaattt
    23641 ataggtttct ttaaccattc gagaaatact tctttaccag tattagttat ggaatatagt
    23701 ttttttaatt tgctaccatc tataacttcg tgatagtgta tatatccttt tgtttctaat
    23761 cttttaagtg caggatagat acttccaaag ctggcgtcaa aaaagtacga ggtactttct
    23821 gacattattt gttttaagtc gtatccactt aattcttttt ccattaaaaa tcctaaaata
    23881 atgtattcta acatattacc tcctggtatc aatcagatat atatcgattc gatatattta
    23941 ttattacaga taataaatat tacgtcaaga tgctttttaa aataagctaa aaagaattag
    24001 gaattattga taaatatgca aaaaaattaa tataaaaggt aattaaatat caatgaagat
    24061 ttaaaaatag ttaaattaaa gtaagagtct aatatataaa atttatagta actttatggt
    24121 ttaaatataa acttgtgtgc tattgacaaa gtataaaatt agcagtaaaa taattgcatg
    24181 agcaaataga ttattgcgaa cgcaaaaaaa tgaggacaat agatattaaa ggagagaata
    24241 tgagagagct attaaaatgg gtttccatta taaatagata caatacaaat tatattgaca
    24301 ggaaattggc ggatattgga atcaataata gccaatattt ttatgtatta cacatatgta
    24361 ataatccagg tattacacag gacactatat ttgagaaaat tcttgtgaat cctagtaata
    24421 taacgagagc tttggcaaat ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa
    24481 aggataaaag aacatggcat ttatatccta cagaaaaatc tttagaatgt tatgatgaaa
    24541 tactaaaaat aacaaatggg tatgttgagg agttgttgag ttcatttgag aaagaagagc
    24601 aagagttgtt tatgtcaatg ttaaagaaag ttgcattttt agctattgat atgaatgaag
    24661 aagcaaaatt ggaggcgaag gaaaatggaa gaaaaatata aggagccatt ggaatatgaa
    24721 aaggtttcaa agttattaac taagtacgca attcctagta ttatagcaat gttagtaagt
    24781 gctttatata acattgttga ccaaatattt attgggcaag gtgttggtgt attgggtaat
    24841 gctgctacaa atgtcacttt tccactaaca acaatatgta ctgcaattgc attattatta
    24901 ggaattggag gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc agaaaaagct
    24961 acaaaagcag caggaaattc tatatgcttg atggctgttt ttggaataag tttatttata
    25021 gtagtatcaa tatttttaac accactatta aaattctttg gctctacatc agaaatacta
    25081 ccttatgcac acacatatac aaaaattaca tcaattggat taccatttct tataatgtcc
    25141 actggaatga gtaaattgat acttgcagat gggagaccaa aagcttctat gttttgtatg
    25201 ttaataggag cagttattaa tacaattttg aatccattat tcatttttgt ttttaatatg
    25261 ggaattgctg ggtctgcttt agcaactgtt attgggcaac ttatatcttt tggaataagt
    25321 gttcattata taaagaattt tcaacatatt aaattacata agaaaagttt taaattagat
    25381 agtataattt ataaacatat atttgcgcta ggttcaagct catgttttaa tcaattagca
    25441 atgactgtaa cacagatagt tatgaataat acattggcat attatggggc taggtcagtt
    25501 tatggaagtg agattccact tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt
    25561 atatctataa ttattggtat atcacaaggt atgcaaccaa ttatagggta taattatggg
    25621 gctgaaaagt ataatcgtgt aaaagaagca tataagtttg cagtgggaag tgcaactatt
    25681 atatctgtta ttgcattctt atgtttccaa ttcttcccaa gacaaattac gtctatattt
    25741 ggtaacggaa atgagactta ttttatattt gcagaacatt attttagaac tttcttgttc
    25801 ttaacattta taaatggtgt tcaaatagta tcatcaactt tctttacatc aattgggaaa
    25861 tcagtgttgg gactttttac ttctttgact cgtcaaattt tatttttaat tccattgata
    25921 gtaattttac caatgtttat gggaattgat ggagtaatgt ttgcaggacc aatagcagat
    25981 gcagcagcgg caatggtatg tatatacttt gcagttcatg aaatgaaatc cttgactata
    26041 aagcaaaata atttgatgag taaccataat aagattgcag aggtataaga caatatttaa
    26101 gaagtataga aaattaattt aagaaatata ataaactaat ttaggaagtg tagaaaatta
    26161 atttaaagaa gtataataaa ctaatttagg aagtgtagaa aattaattta aagaagtata
    26221 ataaactaat ttaggaagta tagaaaatta atctaaagaa gtgtaataaa ctaatttagg
    26281 aagtatagaa aattaattta aagaagtata atgaactaat ttaggaagta tagaaaatta
    26341 atttaaagaa gtatagaaaa ttaatctaaa gaagtgtaat aaa
//
LOCUS       DAEPOG010000034        25978 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEPOG010000034 DAEPOG010000000
VERSION     DAEPOG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.034"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            744..1418
                     /locus_tag="KR432_001242"
     CDS             744..1418
                     /locus_tag="KR432_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639790.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            1406..2566
                     /locus_tag="KR432_001243"
     CDS             1406..2566
                     /locus_tag="KR432_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0639791.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2783..3310
                     /locus_tag="KR432_001244"
     CDS             2783..3310
                     /locus_tag="KR432_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH0639792.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            3596..4264
                     /locus_tag="KR432_001245"
     CDS             3596..4264
                     /locus_tag="KR432_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639793.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            4267..6735
                     /locus_tag="KR432_001246"
     CDS             4267..6735
                     /locus_tag="KR432_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639794.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6975..8399)
                     /locus_tag="KR432_001247"
     CDS             complement(6975..8399)
                     /locus_tag="KR432_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639795.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(8418..9110)
                     /locus_tag="KR432_001248"
     CDS             complement(8418..9110)
                     /locus_tag="KR432_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0639796.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(9165..9941)
                     /locus_tag="KR432_001249"
     CDS             complement(9165..9941)
                     /locus_tag="KR432_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0639797.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9938..10708)
                     /locus_tag="KR432_001250"
     CDS             complement(9938..10708)
                     /locus_tag="KR432_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0639798.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10705..11409)
                     /locus_tag="KR432_001251"
     CDS             complement(10705..11409)
                     /locus_tag="KR432_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0639799.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11664..12176)
                     /locus_tag="KR432_001252"
     CDS             complement(11664..12176)
                     /locus_tag="KR432_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639800.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            12502..12759
                     /locus_tag="KR432_001253"
     CDS             12502..12759
                     /locus_tag="KR432_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639801.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12902..13528
                     /locus_tag="KR432_001254"
     CDS             12902..13528
                     /locus_tag="KR432_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBH0639802.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13778..14557)
                     /locus_tag="KR432_001255"
     CDS             complement(13778..14557)
                     /locus_tag="KR432_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0639803.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14625..15086)
                     /locus_tag="KR432_001256"
     CDS             complement(14625..15086)
                     /locus_tag="KR432_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0639804.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(15062..15739)
                     /locus_tag="KR432_001257"
     CDS             complement(15062..15739)
                     /locus_tag="KR432_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0639805.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15972..16358
                     /locus_tag="KR432_001258"
     CDS             15972..16358
                     /locus_tag="KR432_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0639806.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            16361..18160
                     /locus_tag="KR432_001259"
     CDS             16361..18160
                     /locus_tag="KR432_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH0639807.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(18515..20389)
                     /locus_tag="KR432_001260"
     CDS             complement(18515..20389)
                     /locus_tag="KR432_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0639808.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(20394..22058)
                     /locus_tag="KR432_001261"
     CDS             complement(20394..22058)
                     /locus_tag="KR432_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH0639809.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(22072..22893)
                     /locus_tag="KR432_001262"
     CDS             complement(22072..22893)
                     /locus_tag="KR432_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0639810.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22992..23756)
                     /locus_tag="KR432_001263"
     CDS             complement(22992..23756)
                     /locus_tag="KR432_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0639811.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23753..24502)
                     /locus_tag="KR432_001264"
     CDS             complement(23753..24502)
                     /locus_tag="KR432_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0639812.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(25006..25611)
                     /locus_tag="KR432_001265"
     CDS             complement(25006..25611)
                     /locus_tag="KR432_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0639813.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25790..25888
                     /locus_tag="KR432_001266"
     CDS             25790..25888
                     /locus_tag="KR432_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBH0639814.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         9313 a         4172 c         3139 g         9354 t
ORIGIN      
        1 aatttaataa aaataatgct aaaattaatt taattaaata atactaataa ttactaatga
       61 ttatgctaat tgtcagcatt tactaattta atcactatta gttatttctt ttattaaata
      121 tctttacttt ttttacttat tatgtcttct agctttacat gtggattatg gtatttcttt
      181 ctatttttag tcacatcttt ctattgaatt gcttgcttaa aacaccaatg cacacatgcc
      241 agataatgtt cacaattatg taaaaatatt gctctacctt cttcaatgtt gatattatct
      301 ctcctagtta atattatctt tcctaaaatt tcttttaatt aaaaaacaac attagttgat
      361 atatcaagta tattttaagc aactgaaaaa ataataataa ttgatatatc aactaattaa
      421 tgtagattaa ttcattttta taatttatat actttttaat taatctacct ttttaattaa
      481 aatccttcct ttctatttat ccattttcaa cttaaattat aagatttcat cagctgtgtt
      541 tttgcattcc aataaatatt aaatatcaaa tatatattct attaaaattt ctaataattt
      601 catccttgat ttatttaata tataaaaata tactaacttt aatgattaag ttttgaaata
      661 taactaagaa tttgagaatt tattgagatt tttatactaa aatataaata ctatacaaac
      721 ttaatttaga agggagcatt cttatgaaca ataaaatact cgtcgtagat gatgaggaag
      781 gtataataac actcttaaaa gattactttg aaatgaatgg ttatgaggtt tacactgctc
      841 aaagtggaaa agactcatta aaaaaaatat ccaaaaatcc agatatcatt ctacttgata
      901 taaacatgcc tgaaatagat ggaattgaag tttgtaatag aattagagat tatgtatcat
      961 gccctattct atttcttaca gccagaattg aaaatactga caagataaat ggttttcgtg
     1021 ctggtgctga tgattatatt gtaaaacctt ttgatttaga cgaacttgga gctagagtat
     1081 ctgcacattt aagacgtgaa caaaggaagt caaatcaaac taaaattaaa ttttataatg
     1141 atttaacaat agattacgaa aaaaggttaa tttgtatata taataatcag atttcacttt
     1201 caaaaaagga atttgatata gtagagcttc tctctatgaa tcctggacag gtatttgata
     1261 aagaaaaaat atatgataga gtctggggat acaatagcaa tggaaatagt gatgttataa
     1321 tggaacacat tagaaaaata cgaatcaaac tatcaaaaca tactcttgaa aattatatag
     1381 aaacagtctg gggagttggt tataaatgga taaaataagt aatatgtcat taaaaaaagc
     1441 tttctttgtt cttactttat cttcactaat tatagcatca gtacttacta ctttcgcata
     1501 cattttactt aataatctat ataattctat acaagataag tatttaaaaa cacctataaa
     1561 tacaggtatt ataaccatag ttcaaaacaa tgtgaaccaa tttagtgatt atgataaaac
     1621 attacttaat ataatcaaca tacttcagct tgtacttcca cttatatttt ttataggact
     1681 tttattatta gctgatataa ttttttacaa ggtaaaatta aaaacaccca tagaaatcct
     1741 caataaaggt gcaatggaaa tatccaacaa caatttagat ttttgcttag aatataataa
     1801 taatgatgaa ttaggtaatc tatgtaatgc ttttgaaaaa atgaggtctg aactaaataa
     1861 aaacaacata aatatgtgga ctatgattaa tgatagaaaa caacttaatg ctgctttttc
     1921 tcatgattta agaaatcccc taacagttct aaaaggttac tcaaactatt taacaaaata
     1981 cataccaaca ggcaaactta gtgatgagaa gattttatca accacacaac ttatgtctga
     2041 gcatattaac agaattgaat actatgtaga aaatatgagt aatgctcaac gattagaaga
     2101 tttagttgtt tcaaaatcta tgtcgaatat aaacaagttt atagaaaact tagatgaaaa
     2161 tatatctatc attgctaaac aagaaggaaa atcttttaca cttaaaagtc aaataaataa
     2221 tataaatcta ttttttgatg aaaatatcat tcttagagtc gtcgaaaata taatatcgaa
     2281 tgcttttaga tatgctagaa ataatgtatc catacttata tatcttgaac aagaactcct
     2341 tacctttgta attgaagatg atggtatagg atttagtaag gaatctctaa aattagcttt
     2401 aagacctttc tatagagata agactttaaa tgacagcaat ccacactttg gtatgggtct
     2461 tcatatctca aaaatattat gtgaaaaaca tggtggctct atttctattg aaaataattc
     2521 tactaacagt gctaaaatta ctgctaaatt ctcaacaaga aattaaaatg attttgtaag
     2581 ctttcattgt tcttatataa gctttgttag tttagaattt ataaaatgta tttcaattta
     2641 caaccaatat atatactatt ttgtagaagt tgtaagagtt gcttcaatta taatattttg
     2701 gcaaaaggca tttgaaacaa taatagaaat atatatttaa atatgtagaa tagtatgaaa
     2761 ctagaatttt ggaggtgatt atattaaaag ttttgctaat aatattacat tctctcgtat
     2821 tgtgtttgca ttgctaatgc ttagttctac attctgttca ctgcctttct ggatatgtta
     2881 tttttggtgt ggaattagtg atatacttga tggtcttatt gcccgaaagt tgagacaaca
     2941 aagtggaata ggtgcaaagt tagatagtat tgctgatttc atttttgcgg ttacaatttt
     3001 ggttactgtc ggaaaaaata ttgctcttcc catgtatcta tggtggtgta ttgttgcaat
     3061 agcattatta cggttgatta gttacagtat tggttttttt aagtatcgca cattttcatc
     3121 gcttcatact ttgatgaata aaatcactgg aatttctatc tttgcttttc cattactcta
     3181 tatttgcttt ggtcttgatg ttgcaggaat tatgatatgt atcgtggctt ttctttcttc
     3241 ttttgaagaa atagcaatta tgataaaatc aaaagaatta aatcgtgata taaaaagtat
     3301 atttatgtga caacttagaa tttgtgacaa aaataattta tataataatg aggaagtaaa
     3361 acataaaaaa ttatccacaa gtttatgtgt aaaatagttt taaagcaata gagttgtatt
     3421 gtaaagcctt tagtgcataa attacatttt aaattaaaaa tgatactgaa actgcttatc
     3481 agcactgcga attatccgta aatagtgaag actttttaga tttaagctaa aagttgggaa
     3541 aaaattgagt tttaaaactt acactcttct tatatagaaa aggagagtgt tttttatgat
     3601 tataaaagca aaacaattat caaaaatata tggttctaat aacaataaag ttatagcact
     3661 taataatgta aatttaaaaa taaactcagg tgaatttgtt tctgtaattg gaccttctgg
     3721 aagtggaaaa agcacacttt tacatatttt aagtggatta gataatccta cctctggaca
     3781 agtgttactt gatgataaag atatatataa acacactgaa aaagaactat ctgcacttag
     3841 aagaaaatcc tttggattcg tctttcaaca atttaatctt ttacctgttc ttacagcatc
     3901 tgaaaatatt tcaatgcctg tacttctaga taaaaaacaa cctgataaag actatttaaa
     3961 tgaaatttca tcattacttg gaattgcaga tagactcaat catcttcccc atgaactttc
     4021 aggcggtcag caacaaagag ttgcaatcgc tagagcactt attgcaaaac cagatataat
     4081 atttgctgat gagcctacag gaaacttgga cagtaaaagt ggcagtgaag ttatgaatct
     4141 tcttataaaa acttcaaaac agtttggaaa gactttagtg gtaattactc atgatgatag
     4201 aattgcaaaa cttgctgata gaaaaatttc aattattgat ggtgttctta tggaggtgaa
     4261 atagaaatga agaattacct ttcactttca ataaaagaat taaaaagcca gaaacttatg
     4321 actacattca tcattatagc aatagtacta tcaactatta tgacaactgt ggttggacag
     4381 tcaattggta tcctacaaaa tttaagaatt gaacaagccc gttcttttaa tggtgataga
     4441 catgtatcct ttcaccaact tactaaaaat caagttgatg acataaagaa agatgataga
     4501 gtttatcaag ctggtacttc aataactatt ggcagtagta aaataaagga ttctggtatc
     4561 tctgtacttg taaaagagta tgataaaaca ggactttcta actacccaaa attaatgaaa
     4621 ttaaaatctg gacatttgcc taaagataaa aacgaaatag ccttagatga aaacactcta
     4681 aagcttatgg gtataaaacc aagactagga gtgactatac ctatgaattt agatatttca
     4741 cttttaaatg ataccatacc accatataat tacacagcta actttaaatt gagtggcatt
     4801 ttagaggatg attatacagg atatgtaagt ggtatagtaa atggtattgt aggtaaaggt
     4861 acatcagaaa atctattacc taagagatat attttatcct ctttggattt taaaataaaa
     4921 cagcaagaaa agtttcaaga aatagtaaat caattagcta aaaaaataaa tctttctcac
     4981 aattcaattc aatataattg gatttactta aatgctctta agatacagtt tgaaaaagat
     5041 gaaaatagtt ctaatagtga tggtatatct atgattatat tggtatcttt atttgttgcc
     5101 ttattggttt tactggcttc tggacttgta atctataata ttttaaaaat ctctgttaca
     5161 aagaaaatta aagagtatgg atgtttaaga gcaataggag ctgaaccaaa ccagatatat
     5221 aagatagtta ttttacaaat cttaatttta tgtactgttg ccatacctat tggagctgta
     5281 attggaatta tctcttctaa aggcataact ggaatggtca ctaatatctt aaatccagat
     5341 atacttctag caaatgataa taaagaaatt actgagctta tacataaaaa tacaacagca
     5401 tatatgttcc cattagtatt aagcactaat gtatctttga ttttttcatt tatttcagca
     5461 ttaccatcag caatatatgc ttcacgtgtc tctcccaaaa ttgctatggc tggtagtact
     5521 accaaaatca aaagaaaaat taaaagagaa aaagcaataa aaaactttga aagacatcta
     5581 gcatggctaa acttaaaaag aaataaagga agaactataa taactatact atcattattt
     5641 atgagtataa cagtattcgt tgcattgagt gagttctcta atgtattaga tgtatctagg
     5701 tcagtaagca atttaaaaga aggagatttt tccttaacaa atgaaatatc tggttttgat
     5761 aaatcaacac tagataaaat agataaaatg aaaaacgtca atcgcacttc ctttataaag
     5821 tactctgaat acaaacaggg tgaaatagat acagatataa attttgaaaa tagtggtgag
     5881 atgttaaaaa tcattggaat tgacgagcag actttaaaag acttaatgcc ttctattaca
     5941 gattctatac tagaagactt taaaaatggt tctatctgtt ttataaaaaa tccgattgct
     6001 atttctacac caggtgtaaa aactagacat acaaatttaa aacctaaaga tagtatcaca
     6061 atcaacaaaa aacaactaga tatatatagt acagtagata aaatgttctt tttacaaggt
     6121 aatggctgga taaatggtgt tgatgttatt gtatataatt ctgtttataa caccttgaca
     6181 aataaaaaca agttcaacca gataaatatt tatgctaaag ataaatcaaa attagaacaa
     6241 ataagattgt ctattgaaca gatttgtgaa aataatcctg gctctcattg gatatcatat
     6301 attgaatcag ataagcaact taaagagagt tttaaacaaa tagaatttct agcatgggca
     6361 gttatattat ttgtgggatt gattggtact ttaaacatca ttaacacaac tcatacaaat
     6421 ataaatacta ggactaatga gattggagtt aaaagagcta ttggtatgag taatattagc
     6481 ttatataaaa tgtttttatg ggaaggtgtt tattatggaa tttttgctgc tatatttggt
     6541 tcaattgctg ggtatgccag tgctattatt ataaacatgg caactataga aaagcttgat
     6601 ttcactaata taccaattac ttcaatttta caagctacta ttatttctgt acttgcatgt
     6661 attatagcta ctcttattcc gttaagaaaa gttaaaaaga tgaatataat tgactgtata
     6721 gatatcaatt tataactatt ttacagataa taatataaat taaattatca atgactgtct
     6781 ctattttctt tcaaatctaa ctaataaatc tttttaacta taatataagc tttaaaaaag
     6841 gcctgtctca aaatagagat aagccttttt tcattgtcgg aataatgaaa atatatttaa
     6901 cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc tttgagacac tcccctttaa
     6961 tttaaaatat atagttaaat gctatgccaa aatttcacac atgctccacc tgattttgaa
     7021 ttttctattt taacagaacc tccaagctgt tccactaatt gtttgacaat gtaaagccca
     7081 agaccagaat gtccaccttt tgtctgccta gcttcatctc ctcgataaaa cttatcaaga
     7141 gcctttttta aatctttaga gctaaaccct ctaccagaat cacaaatctc ataagaaata
     7201 caattttttt catcctttac agtaatacta atattaccac ctgatggagt gtattgcaga
     7261 ctgttggata tgatattatc aaaaattcgt gtcaacctat ccacgtcgat ttgaattggg
     7321 gattgtatat tccccttcat tttcaatata agttcaactt ccttttgatg agcctgtaat
     7381 tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat ttataggaac aagatttaat
     7441 tgtatatttg acttttctaa gtcagacgta tattgcatct tttggacaag agcagcactt
     7501 ttttctgtat tttctcttat cactgtaaga tacctatgaa gtttttcatt gtcatctgta
     7561 ttattaccaa taagtgcatc tgtatatccc aaaataatag aaagaggaga ttttaaatca
     7621 tgtgctagag cttccaccat ttcaacacgt tcctgctcca tcttccactg cgcagataaa
     7681 gaacctttta attcgtcttt catttcagaa aaggcactac aaagtttacc gagttcatta
     7741 tctgaataat agtcgatttc aaaatcaagg tccttttctt ttatcttctt ggacgcatca
     7801 atcaatagat gtaatggttg attgatattc tttacaaagc gcttagaaag ccatctagta
     7861 aaacccacga tatatataaa tggagaaaac agagccatta taataactgc aaatacaaat
     7921 ctccctctac tatttgcaaa ggttattttg acttgataga atagaataac agctccctca
     7981 atctttccat tatccccttt gattggaaca gtatggatat aaccaccttt acgaacagtt
     8041 ttattgataa aattattaaa taattcttct tttgttttaa atagtttttt ggggttagta
     8101 ccatatagaa tattgccatt attatccaca acttgatatg gcatatcatc cccacgaatc
     8161 gttcttttta aaccttcttc attcgattgt gataacaaag ttatgttttt ttcattgatg
     8221 tatttttcaa ttctaggaac ttgtcgctca taatagtttg gtggatagat atccttattt
     8281 aaggaatgaa tatacaaata gagagcaaat acataagtaa tcactgtggc aactatactg
     8341 gctattataa taaaaataaa agtatgtcta aattgggtaa taagggattg atttttcatt
     8401 taataacctc tctctcttta cattttattc cacttatacc caatacccca aactgtggaa
     8461 atatattctt tttctggtgt cacagcagaa aacttagctc gtattttttt tactcgttca
     8521 acaacagttg aggaatcacc ttcagaatca cacccccata ctttttcata gatttgttct
     8581 cttgagaaaa cttgtcctgc atggagtgct agtaattcaa caatatcata ctctattttt
     8641 gttaaatcaa tattcttacc atcaatttta attgcacgtt ccttcatata taagcataac
     8701 ttcccaaaat acaaagtaga tctcttgttc tcttcattga tatactgtga acgcttttcc
     8761 ctacgcagat tagcttcaat ccttgccata agttcacgca gtccaaatgg ctttgttaca
     8821 taatcatccc ctccaaaggt caaccctttg attttgtcgg tttctgattg ctttgcactt
     8881 aaaaatataa ttgggcataa aacatcatct ctaattgcct gacatacctc aaatccattc
     8941 attttaggca tcataatatc cagtataata aggtcaggct cctgttttga taactctata
     9001 cctgcctgac catcataagc tatcaacact tcatgtcctt ttgccttcaa ttcatcctca
     9061 agtagcatta ctaggtccat ttcatcatca ataatcaata ttttactcaa tataacacca
     9121 cctttctctt atgggaaggt cagtattaaa acaatattcg tctactaata acttttcctt
     9181 ccctcccaca ttttaaacca aataacgcct cctatcaaga atataatcaa acctattgct
     9241 actactataa gctctatact agctgtttgc atcactttag agtaaaatgc ctcagttgaa
     9301 atagttccag cttctttaat aaaataaatt cctggaagca tacctagttt tactggccat
     9361 gtccatggaa caaatgccca tactttttca ccaacgctag ttaatccaag tattgctgcc
     9421 atcaaaattc caccgatact aataccaata gatgcaccca taccccatgc aaaactcacc
     9481 caaagatgaa tagctaatag tggaagtgtc ccaatcacca caagtcctgc tgctgacagg
     9541 aataaccata ttgttgctcc agatggcact gcaatactca ttcctatgca aagtatcaac
     9601 gtagcaataa atgtgcatat agttaggcaa aacagaagaa aaagaaattt tcccagatat
     9661 aatctaacac gagaaatacc tacacataaa aaaccattaa aatttccagc aagctcttcc
     9721 tcctgcacta taaatcctgc taaaatacct actccaatag gaatgatgaa tacagtccat
     9781 actgtaaaaa atccttcaaa agcaaattct tgtgtacttc cagatttagt tgtgagataa
     9841 aaaaccacac ataatgaaaa aataataggc atacaaagag taagccactg gacagctgtc
     9901 ctctttgctc gaagccattc tgatgataat agctttatca tttgtcatcc ctcctattga
     9961 accaagctgc agtaagcgct gttaatacta taaacacaac caaagaaaca acaatcccaa
    10021 taggaatcac tgaagtattc aaaagtgggt ctccagcttc taatatagta ccgttaggat
    10081 gtatgttgat gattgggcat accattctaa ctgcccaact ccaaggtact gcaatccaaa
    10141 atggttttgt cgcagcaagg acaccaaata tcatacctaa agctccaact cccatactta
    10201 ggaacatacc tttccatgta gctagccata gctgaagtgg aagtatagca agagaaacaa
    10261 cccagcatac aatacttgcc cctataatct gtccaaaagg aactggacca gctttcaata
    10321 ttaaaccagt tataattgtt acgactatta aaactaacgt tgaaataaaa ctgtagactg
    10381 ccatagctat aactttatta atccaaagtg tcataggaga cacattgtgt gtacgtaaag
    10441 cacgataatt tccagctttt ttttcctgga aagctaccaa agtagcaaac aatgcaaatc
    10501 caataggcag aaataaaaat gaccaccagt taaaaattaa tgctaaaaga ctttgccacg
    10561 cccaagaagt tgtttgagat aatggatttt gcatgagtgt tgattgcatt ataaatgcat
    10621 atgctgcaaa agacactggg aaaaaaacaa taagtttacc cataaatgtt cttttatact
    10681 ttaaaagttc tgattgtaaa gcattcatct gttttgtcct cctttaagaa taacatctgt
    10741 aaaaaattct tctagatttt cattagggtc aggcattcct tgaaatagta gtcgtcctcc
    10801 atttataatt cctatattat ctacaacttg agcaacctct gatagaatat ggctagaaag
    10861 aattacagtt ataccttttt ttggaaaaga agcaatcaat tcacgcaatt cttgaatgcc
    10921 aaaggggtca agaccattgg ttggttcatc tagtattaac aactctgggt cactcaaaag
    10981 tgcaatagct attcctaacc tttgtttcat acccatagaa aattgtgaag ctctcttttt
    11041 acctgtattt ttcaaattta ctatttccaa cacttcataa attttatctt taggtattcc
    11101 caatattttt gtatgtacta acaaattttc ctctgctgta aggtttccat acaatgcagg
    11161 ggattctatt aaagaaccta tttttgcaag agattctcgt ttccaaggct ttctatcaaa
    11221 aataatacga ccatctgttg ggcgaagaag accaacaagc attttaagtg ttgtggactt
    11281 tcctgctcca tttggtccta aaagtccata tatactattt cgttcaattt gtagagaaac
    11341 attgttaact gccatttgtt ctccatatct ttttgataag ttttttgttt caagaattaa
    11401 atctttcata ttaatttctc ccttcttgta ataccttaaa tctattacta agagaatact
    11461 aacatgaaaa aataacaatt ctataacacc tactaatttt aataaaaatt aaaataaaaa
    11521 atagagttac caattttatt ttggcaactc tatttaagtt tatgttttac agttatatta
    11581 gatttaagct tagtttcatt atcacactaa cttcaaattt actctataat ccttaaatca
    11641 agcactaatt tattaatcaa acactaatct attaatttat atttctttag acatctaata
    11701 tagtttgcta ctaaataata attacttata tcatatttat ctaatccttt aaaagaagtt
    11761 atttcatcat taagctcatc actaccttct acaaaaacac aacctgtttc aaattccata
    11821 cctacaataa tcttacagcc actataagtt tcctcttgag tatcgtacaa tttttttgca
    11881 tttccttcat ttggaattgt aaatacaata ggttttaatt ctttatcact ggaaatcttt
    11941 tgtgaaaaag gcttgtttaa cttaatatta ttgataacta catgtttaaa ttcctctgaa
    12001 gtttcttcta ctattacata ttttcgcttt aaaaactcta ttatttctaa aacagactta
    12061 gaatttactt ttaaaatact tttatatttt tcatataaag atttccactg ttcaattttt
    12121 tcctgtggta cttcttggat atgtgtactt attttttctt gagatatttt tttcaattaa
    12181 actctccttt caatacttaa atatccttat aaatctcaca tgactaaagt tacaaggttc
    12241 ctacttaata gaattttgca tacttaaata tgtcttatta attctctaca ggatatctcc
    12301 gtagtcccta cgattcttac ataagttacc tattttaaat tgttattagt tagattataa
    12361 aaattattta ttttatcact aatataatac tcttaaaaac tttacaaatc aatttaaatt
    12421 tgctaccttt tatttataac aacttatagt acatttctcc tatgcttttc aatatattac
    12481 ttttaaaagg aggttttcct tatgatttta aatagaactg agcttttccg aggatattct
    12541 ttaaatagat ttaacgagat atgcaatata ttatcagtaa ataaaattaa atataagtat
    12601 aaagtgaaaa ataatgctta ctctaaaaaa cctttttcta atgaaataac acttggcaat
    12661 ttaggtcaaa aggaagattt ctcttatgag tacttaattt atgttcataa agacgattat
    12721 gaacaggcag agtctctaat aaattctaag ttgcgataga ttatttataa aacatgacat
    12781 tctgttgtca tgttttatat gttattatac tttcaaatat gctactatat tttcaaaatt
    12841 gtgtgcttta aaactagctt tttatagtaa tttaaagctt aaaactaagg agactcgtta
    12901 tatgaaaata aatataaaat caaagttaag tgaatttatt aaacagaaca atctttttga
    12961 tgacaacaga ataatatcct accttcctaa tataacaata gaaacagaca aaataaaaac
    13021 tataatgatt aatgaggttg tcccaactaa caccaatgac gatttttata gtgtagataa
    13081 agatgctgat tatttaaaaa ctactattcc tttatttgac agtgcaggaa tagaagtatc
    13141 taacataaat gatatcctag atatgggtat ctatataact aatgcagtaa aacttcctaa
    13201 atcagaatat acaataactc gtgatacaat taaattacat atgcctattt tagaagaaga
    13261 aattaaatta tttaaaaacc tagaggtagt aatgttaatg ggagatgttg caaaaaaatc
    13321 ttttaatatg attactaaaa agcatataaa aaagaatgtc atcccttcca tttcaacata
    13381 taaacttcgt aataatgaac tttattatga taatttaaga gtatttccat catatattat
    13441 gactggtgga aacatactga ttgaaaaatc aaaatttgaa atggcatcag aagatataaa
    13501 aaaaatgttc gagattataa acggttgata ttacaaaata ataaaaacta atttagccaa
    13561 ataataagtg cctcaaaaac ttctatttat gaggcacttt tattttatat tagtgtaact
    13621 atatactatt aacataattt attatttata ttgttctttt taatctctat tccatttagt
    13681 ctaggtaaca ttactcttta caagattact ttatatttct tttatatctc taacgttttt
    13741 atttaataaa acaattatta atacaagtca aatatatcta tttatctaaa aatatatctt
    13801 ttacagtaat atctggatgg tgatacttct tcctattcgt taaaaaagat ttttttacat
    13861 taatagcctc ttttggacac cagtgaaaac aagctaggca tctttcacac tcattacccc
    13921 actttggctt tttatcaact atacttatat tctctacagg acaaaccctt ttacatgtcc
    13981 cacatccatt acatttattt tctgcaaaaa atttagtagt tgcaatttgc atattcttac
    14041 ctactaaacg aagtacaaca ctatctatat gtgactttaa attgtttttt ccttctatat
    14101 ttccaatctc caagttattg atacattttg caatctcagc taccctatta gtctgatttt
    14161 tcaatctttc ttcttcaaca tctgaaggtg ttatcaaagc atttccaggc atattgatta
    14221 aaaaaccagc agataatttt ttcccccttt ttttaagtaa cttatctata gtaaataaac
    14281 tatgtccagc taccccatta caagtggcaa tggcatatat atgagtatta atattaaaat
    14341 ttaatttctc tacaaaagac tttattatat caggaacatc agcaaaataa acaggaaaaa
    14401 caattaccaa tatatcttca tttatgtcta tattattttc ttttctatgt atagctaaag
    14461 gtataacttc tttacttcca tttaacgttt cttttaaaat ctttgaaact actagcgaat
    14521 ttcctgttcc agaaaaataa taaatcttac tattcattct tatccctctt tctatatttc
    14581 tacatcatca atagttaaaa agtctcttaa aataggattt atatttactt tccaattcta
    14641 tccaaaatat cagtaaacaa aggcaattct tcatcagaga tagatttaaa tgtctctgtt
    14701 gcatatatac ttgcaatttc atttaatggt tcttctaatt ctctacctaa atctgttagg
    14761 aatatttgaa acttacgtct atctttaaca tctactttac ggacaattaa tcccttattt
    14821 ttcataacgt ctaaaattct tgttgtatta gatttatcct tacatgattt ttcacataaa
    14881 tctatctgag aaattccatc ttgctcccat agaaacctta ataacgcata atggtcagtt
    14941 gttatatcat atccattttg tttaaacatc ttttttaaat catcttttac ttttaacgaa
    15001 gtacttacta acttagttgt agcttcatat agtttctcaa aatctttcat tttacctcca
    15061 tttaaataat cccctgctcc tcccatctat caattattcc cttatattgc tcaatatctt
    15121 taaaagccat tggctttaaa acagcattta tagcaataaa attatagatt tgagaaagca
    15181 caggtggatt tttcttttcc tctatgcaat aaataaattt tttagatacc ttttgtatct
    15241 ttgattctac tttttcagtt ataggatttt gattaaatcc agtttttata gataaactac
    15301 cacaaacata acctcccgaa tctaagacca tggattttat aaaagaaatt gcttttgaac
    15361 cacgtgcatt ttcatatgta gtaactgcta tacatggctt acgatacaat aatctttcaa
    15421 gcatcatatg tgctctatct gtaaacacct tgaaaagtcc agttacacta cttccataag
    15481 ttgggctacc aaatataaca ccatcactct tagtaattat atcatgaata tactctactt
    15541 tatcattttg attgatacac ttgcccattt tataacaatt taaacaacca atacaatatg
    15601 atggatttac ctcacttaaa tcaaaataat taatttttac atctggtatt aatctttcta
    15661 tatctttata caagtaagtt aaaacttttg atgtagctcc attttttcta gggcttccat
    15721 ttataactgt aattatcatt tctatatctc ctttttagtt gttatatcaa caataagtat
    15781 atcaactata tttttagctg tcaatataaa ccatttattt aaagctatac ttacacagct
    15841 taaataaaag ttttatttaa ctacatgtgc ttatttaatc aactataaat gaattatttt
    15901 taataattga caaactaaat taatgataat atactttatg tattacaaat gtaagtagaa
    15961 ggaggtatag tatgaaaaag ttaccacata tttcagaagc agaatatcaa gttatgaaaa
    16021 ttatttggaa atatgctcct atcagcacta ctgaagtaat tgaaaagttg gtagaaacaa
    16081 gtacatggag tcctaaaaca attcaaacaa tgttattgag attagttaaa aaaggagctt
    16141 taacatatga aaaaaatagc agagtattcg tttatactcc acttgttaaa gaagaagaat
    16201 acgtagctac agaaagtagt tcttttttaa atcgttttta taatggtact cttaattcta
    16261 tggtactaaa ctttttagaa aatgataaac tatctgaaga tgatattgag gaactgagag
    16321 aaatcttaaa taaaaggacg actaaggggg ataaataaat atgtatgccc cattctttat
    16381 acgattttta ctcagtacat taaccttatc aatattaatt gttataattt tacttgccaa
    16441 aaaagtattt aaaaaacata tttctgcaaa gtaccaatac aatattggat ttatattgct
    16501 tataatgtta ataattccat ttgtccctct taaatatatt aatcttggaa atgtatttaa
    16561 ctatttatat acttttaatc aacttggaag cagtactaat aggacttcta ttataaaaaa
    16621 tagtccaaat tctataatta ataactcaaa tgcattaaac gatttttcag tatccctaaa
    16681 ccaagttaat tttgatagtt taaatatttt attactaata tcatggataa ttggaatttt
    16741 aatcatgata gctattacta ttcattgcaa tattaaaata aaaaatatga aaaaatcaat
    16801 acaaatttta agagatgagg agatagtatt tatatttgaa aagtgtaaaa aagatattgg
    16861 tataaataag gattttatac tatgcaaatc acctattata aatacaccta ttacatttgg
    16921 atactttaaa ccacacatta tattaccaga taagtctata tctaaattat ctttaaaaga
    16981 tataaaatat atcttacttc atgaattaca acactttaaa aataaagata tactgataaa
    17041 ttatataatg tgcttccttc aaatattata ttggtttaat cctcttgtat ggtatgcatt
    17101 taaagaaatg cgtatagacc gtgaaattgc atgtgatatt tcagttttaa aaaagcttga
    17161 taaagatagt catatagaat atggacaaac tctaataaac tttgtggata atatttcaag
    17221 accatcaaat cttacactta catcagatat tggtgggtca aaaaaacaaa taaaaaagcg
    17281 actattgaag ataatggatt ttcataatga atcaaatacg ttaaaattca aaagcatttt
    17341 aatttttaca ttgatagctt taattgtatt tggaaatatt cccaaactat ctattatggc
    17401 agatatcaac gaaaaatata gacctacaaa tgaacaaact gtgtacgagg acttaagtca
    17461 gtattttaaa ggttttgatg gaagttttgt tatgtatgat ttaaaatcca agcaatatca
    17521 tatttacaat aaagaaaaaa gtaaaacaag aatttctcca aattctactt ataagattta
    17581 tagtgcctta ttgggattgg aaaatgggat aataacaaga aataattcac atatcccttg
    17641 ggatggaaag catcattcta atagttcttg gaatgaagac caagacttat tttctgctat
    17701 ggaaaactcc gtaaactggt actttcagac tctagacaaa aaaacagggc taaaaaacat
    17761 acaaacttat cttaagcaaa ttggatatgg caattatgat gtttcaggtg gtatctcaaa
    17821 cttttggcta gaatcttcat taaaaatatc acctattgaa caggtagaat tacttgaaag
    17881 tttttataca aataagttta aatttgaaga taaaaatata gaaacagtaa aagattcatt
    17941 gttaatatca aaaaaggatg gggtatcact ttttggtaaa acaggcactg ctaatataaa
    18001 tggaaaagat gtaaatggtt ggtttatagg ttatgtggaa aaaaatgaaa atacttattt
    18061 ctttgctaca aatattcaaa gcaaagatta ttctaatggt agttcagcat ctaagataac
    18121 attatccata ttaaatgaca aaaaaatcta taatccttaa tatatttact gtacattcta
    18181 aaaaattcac ataattttca tattaattat cgattaaatt catatacaag gcttattata
    18241 taaacataga agatagtaaa actagcttaa aaacataata taatacccta tacaaatagt
    18301 ggcaactctc cctcacatcc tcaattgttg ccactattta attttttgta tgaagttaaa
    18361 tatattttca tcatttcaac aatttgggtg cagtacaatc tctattttga gacaacctct
    18421 ttaattgttt tagagtttat gaattatttc tcatacttta aaaagattga attactcttg
    18481 ataatcttct tttgtagtca ttacatatca cctactactc aacacatatt ctttgaccta
    18541 tttttacatc cttgtttata tccaaaatat ttaccttctt accatctgga aacaatacca
    18601 ttgtcaaagt agactttcct aattcatgta ttctctttaa atcaacatta acaagaacat
    18661 ctccctgttt aaccatatct ccttgtttaa cagcaatatc aaagccatct ccatttaatg
    18721 caactgtatc aattccaaca tgaattaata cttctccatt agttccttta atcccaaatg
    18781 catgtccact tgaaaatact gtttctaatt tacctgaaat aggagaaaca atttttccat
    18841 ctataatatc aatagcaaaa ccatcaccta acattttttt agaaaatgtt tcatctggta
    18901 cattttctaa tgcaacaatt ttccccttag ctggagacac aaagttttca ttattttcat
    18961 taaacactaa ttcttcgtct tcatcttcat ttatagcttc tttaggcact ccaaataaac
    19021 atgttacaat aaagcagaat gcaaccacaa atattgatac taataagtat gtgattaaat
    19081 tatatccatc atagatatac attaaataag aaggtaaaac tgctaatcca tatgaattag
    19141 attgaatatg taaaattgat aatattgctg ctcctaaacc tgaagatgca cacataataa
    19201 tcaaaggctt taaattatat cgaagctgaa tcccaaataa aacaggctca cttataccaa
    19261 aaagttgaga tagcattgct ccaactgcag tttgtttaac ttgattattt ttagattttt
    19321 tcataaatgc tagacatgtt ccaatattcg caaatccata cattgcacaa agagtaataa
    19381 gtgcattaaa tcctgtattt gctaatagtg atgtttcaat cattacataa gtatgatgta
    19441 agccagtaat aacagctaat ggatatgtaa atccaataat aaatcctcca attcctaaag
    19501 gtaaaccaat caataaactg atgatatcaa ccatttttaa ttctattgta tgcattatag
    19561 gccctacacc taacaaaata actaacattg ttattagcat aacaaagaat ggtgtgaaaa
    19621 taaggtctaa tacattaggc atatgtttac ggaaccattt ttcaagatta gctccaataa
    19681 atgctgtaac aatagctgtt aaaacacttc cttgacagcc tacaatagga ataaatccaa
    19741 atgccataat tgcttcaact tcatttgatg ggtcagcaac agaatatgca ttaggaagta
    19801 ttggagaaac taacataagt ccaataacaa gtccaataac aggtgttcct ccaaatacct
    19861 taaatgctga ccatacaata attgctggta aaaaagcaaa tgctgtttct gtaagaaccg
    19921 aaacaagtgt ctgaatatac aatggaatat ttgcagaaga cattccaaat aatccaagta
    19981 cattatcatt aaacaaacaa ccctttaatc ccaagaataa tcctgttgcc gcaataactg
    20041 gaataatagg aacaaatata tctgctagag ttctcataac tttcttaaat ccagtttcat
    20101 tatttttaac taattcatct tgttcttttt ttgataaagt tttcaatccc attttttcaa
    20161 cttctgcata aactttattg actattcccg ttcctagaat aatttgatac tgtccagaag
    20221 taaagaaaac acctttaacc tcattaacct tttccacttt cttatcatta ataatttctc
    20281 tatctttaac catcaccctt aatcttgtgg cacaatgtgt aatagaaata atattatttt
    20341 caccaataat ttcaataagc tcatttgcta ttttattata tttattcatt tttctacctc
    20401 actttcaata atactgcttg aaatggtttc aaactcactg ttttatttag tagatttata
    20461 tcatctaaat tttggaatac aatatcatat ttttcttctg tttttatttc tacaagttta
    20521 tcagaaaaat tataataaca atcaataact accaaatcta attgccttct atacgcaata
    20581 acattgtcac tttctatatc aataggttct attgaaccat aaatcaaaca atcagaatac
    20641 ttactatctt gtctcaactt tatcatcttt ttataaaatt ctaaaatact ttctgaattg
    20701 ttaatctgac tctgtgcatt aatatcttta taattttcaa taacctttaa ccatggtgtt
    20761 gcatctgaaa aacctgcatt ttctttatct gcccatggaa atggcgttct tgcattatct
    20821 cggcttctta aattcacaaa ttctaacgct tcttcttctg agtaaccttc atctattgaa
    20881 cgatagtatt ggtcaatgct agataaatca ttaaaatcat ttatggtatc tcttttaaaa
    20941 tttgtcattc ctaattcctg tccctgataa ataaatggtg ttcctcttaa aaagaaatac
    21001 attgctccca acatttttac tgcatcatca ttcctttcgt attgcaaaag atactttgtt
    21061 gtagctcttg gttggtcatg attttcaata aaattagctg cccatccatg ttcctgaaca
    21121 tacatttggc tatctaaaat gcattttctt aaatctggaa ttgtccatga aattctttta
    21181 aaccactcac taccagaagc aacatctaag tcagcatgtt taaagtcaaa gaccattgaa
    21241 aagaaaccat cttctccaat aaaatcacct agttggtcat agctaatacc tgctgcttca
    21301 gcaacagtca tacaatcata ttttttaaac gtattttctt ttaattcata aagcattttt
    21361 tctattccag gctgatttct aactgttttt gtacatttaa caagaccatc aacaccatca
    21421 ctttctaaac tttgaaaact taaattcttt ttgataaatg taatagcatc aactctaaat
    21481 cctgccactc ccatttgaga ccaggtatta atcatttggt aaagttcact tcttaatatt
    21541 ggattttccc aattaaggtc aggttgtttt ttaccaaaag aatgaaaata atattcgtct
    21601 ctattctcta ccttttccca aacacttcca ccaaaaacag acctccagtt atttggtaaa
    21661 tcgtttcctt ttttaaaaat ataataatca tgataaggac tttctggatt ttttaatgct
    21721 tcttgaaacc aaggatgttc atcacttgta tgattaagaa ctaaatctaa tataattttg
    21781 attcctctat ctttactttc tttaattaac tcttttaaat cttccatact tccaaattca
    21841 cttgcaatat ctaaataatc actaatatca tacccattat cgtccatagg tgatttaaaa
    21901 attggacata accacaatga tgtaatacct aaatctttta aataatctaa acgagaaata
    21961 attccctgta aatctccaat tccatcatta ttactatctt gaaagcttct tggataaatt
    22021 tgataaacga tttctttttg ccaccatttc ttttccataa attactcctt tttatatgat
    22081 atttcttctt tttaattctt tataaattcc attatcgaat atgtcctcac atatagatgt
    22141 cgctatatct ttaagttgtt gatgactatt tttcattgca attgtaacgt ctgatgcttg
    22201 aaacatgaca atatcatttt gaccatcacc aaaacatatg gtttcttttt gagttacccc
    22261 taatctttct tgtaatctct taattgcttt gcccttatga aaatcttttt gaataatttc
    22321 ataatactga ctagaaatat ctctctgtgc taattccatt ttatcttgta gtatatcttt
    22381 cacttcgtca aatacctttt catttgacca taaacaaatt ttatgtacat cttgtgactt
    22441 atactcttca atattatttt catatgttat tttttcttgg atatgttgtt tatttataca
    22501 agaattagtt cctttcaact gattcattgt ttcaaatatt tctttggctt tttgattcat
    22561 gaaaactttt tcctgactct caatcgaaaa agcaacttct ctctttttta acaaacaaac
    22621 aacttccttt atcaatcttt gattaaaaga ttgattataa agtaattcac catgatattg
    22681 aatgtaatta cctcctcctg caatatatcc atcaacccct aatgataaaa catcatcttg
    22741 tattgtacct atacttcttc cagtacaaat aacaacactg caatgattct tttgacataa
    22801 acgtatagcg tgctttgcag attctgggat tccatatacc tcatccctaa gcgtaccatc
    22861 tatatcaagt attaataatt tgtacaattt catcttcctt tcttactatc aaaatcataa
    22921 tagcattcaa aaaaaaagat atcaatattt tgatatcttt taaaaaatat gttcattttt
    22981 agcacaaatt ttcactttcg gggaatatgt tcactttcta aatacatctt gtatttaata
    23041 taaagcagtt caattaaaat aaaataaggt gttgttacat cgtagtccag ttgtttatct
    23101 acagaaatag atatagattg aatatacagg ttttcatcac ataatcttgc taaaggatta
    23161 cttatcattt ttgtaataga aaccatataa gttcctttca ttctcatttc tttagcaaaa
    23221 tcaataattg tagatgtatc tccattcaat gagataaaga aaatcatatc atctgttttt
    23281 ataaaatgat taagcgcctg aatcatatca taaccataaa catcatatat aattttccct
    23341 gttggtaaga aaatcctttt catctcttta acaactcttt cctgagaata accttctcca
    23401 aagacgatta tacgatttgc ctttttcatt ctttcaaaca taatcgaaca atccttcttc
    23461 tctatatcat ctatcacctt attataacaa tccattaatt ggtgtttact ttgattttta
    23521 tattctactt ctgtttcatt taagcgtata ataactctta attcactata tccaggtaat
    23581 tgtagttttt gagcaaaacg agaaagtgca cttgtagata tatggtattt gtctgcaaat
    23641 tcatctatag aaagtttaat acaatcttct ttatgattaa gaatacactc acagatatac
    23701 ttatcatttg ctgtaaatga attataatat ttattgaaaa gtccttcaat tttcattctt
    23761 ttctccttct atactctatg taattcatat gaagcaaatc taatagagca taaaatgctc
    23821 ctacgacttc ataagatata gcttgtaatg tatcaagttt ttgagtttca acatacagat
    23881 tatactcaca aagtgttgca attgtattat tatcaagcct agttaaagaa atcgtatgta
    23941 tatgattgtt gataaatttt gcaatttcaa ttcctgattt tgtttctcca gacaaagaaa
    24001 tgataataaa taaatcactt ttttgaaatg attctctcat aaattcaatt tcacctatat
    24061 caaataagtc aataacacat ttacctactg aaagaaatat tctctttatt tcttgagcta
    24121 aacttttctg ttcattacct gtcccataaa tataaattgt ttctgcatca tatattattt
    24181 tacaaatatc ttgaaatgat attttttgta attcattcat taatagtcga taattctcgc
    24241 aaacatcatg aaaattatta tcattattaa caaattctag ttgattattt ttaagtaaat
    24301 actttaaatc agagaatgaa tctaatccaa tttttctaca aagccttaat aaagtagctc
    24361 tagaaacatt acattcattt gcaatttttt cacacgaaaa attacggtat tctctttgac
    24421 aaatcaatat ttgtttaata ataattttat cattttcatt taaactatta taactttggt
    24481 taactaacga ttctaatctc attttttcca ctccctaaat attattttaa tatacattag
    24541 ttgctttttc tagtgaagag tcttaaataa taaggataaa ataatcattt gattttattt
    24601 ttattaaaat aaaaataacc tgctaaaagc aggcaccaaa aagtacttat cctttcgaac
    24661 atgtacttta gatattgctt tcttcggaag cttcatctct tcatactcta aaatagagtt
    24721 ttttctaact caaaagtatt ttccatggta gaaattactt taattcatct gccatttatt
    24781 ttttatctta tttaataatt actattactt gaatatttac ttctttaagt aaatttcata
    24841 gttaacaaag ctaaaactat tattatagat gttaaaatac aaaattataa gtattgttat
    24901 aaatatgctt acagctacaa atatacatga tatattaaaa agaagctgta aattattatc
    24961 atacatacaa gcaactatgc ctctattaat ccatgttaat ttttattatt taatacaagg
    25021 agttccatga gacatacata tccattcata ttttaaatta tccaattctc ttatagattt
    25081 catcatcttt ttataatccc aattccatgg attaggataa ggaactatat ttccattctt
    25141 atttttgact aaatcacctg caaataatac atcttcaaaa atcatgcaga catgccctgg
    25201 agtatgacca ggagtatgga taatttttat attcccaatc ttcatattct ctccataagg
    25261 ttcaacatcc ttaatgagcc ttctggaaat tgcctttcca ataaacttct taaacccagg
    25321 tctatccttc tctcctataa taaaaggtat atcctctaca tgagcccata cctttgctcc
    25381 tgttttctct ttcaataatt taatattaga tatatgatca acatcatgat gagttaataa
    25441 aatatgctta atatctgtaa gtttaatgcc aagtgttttt aattctttaa ttatattttt
    25501 ccccataaaa ggaagacctg tgtctataag aataatttct ttatcgaaca caataaaaca
    25561 atttgaacca cgagtactat ctaacattaa tacattttct tttatcttca taaataaatt
    25621 ccccttattt ggtattttac ctatataatt aattaataaa gttataaata gttataatac
    25681 ttgatattaa ttttattttt tattaaagta tactataggt atacaaatat tatatttaca
    25741 taagtatacc atcagtatac ttttttatca atagaaagga ataatttata tgtcagaatt
    25801 atcaaaaaga gataaagaaa aaatacaaag agaaaatgag attattgata aggcagaaaa
    25861 attattttgt cttaatggat ttgattaaaa aatagctacg cacactcgtg actttagtca
    25921 tgagttagta gctaaaatag tcagcatata gggaaaccta tatgtagaga taggataa
//
LOCUS       DAEPOG010000035        25942 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEPOG010000035 DAEPOG010000000
VERSION     DAEPOG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.035"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(123..2342)
                     /locus_tag="KR432_001267"
     CDS             complement(123..2342)
                     /locus_tag="KR432_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBH0639815.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(2606..3703)
                     /locus_tag="KR432_001268"
     CDS             complement(2606..3703)
                     /locus_tag="KR432_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639816.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(4019..5212)
                     /locus_tag="KR432_001269"
     CDS             complement(4019..5212)
                     /locus_tag="KR432_001269"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH0639817.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(5349..5452)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5641..6150
                     /gene="yyaC"
                     /locus_tag="KR432_001270"
     CDS             5641..6150
                     /gene="yyaC"
                     /locus_tag="KR432_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBH0639818.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(6261..6689)
                     /gene="fabZ"
                     /locus_tag="KR432_001271"
     CDS             complement(6261..6689)
                     /gene="fabZ"
                     /locus_tag="KR432_001271"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH0639819.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(6705..7724)
                     /locus_tag="KR432_001272"
     CDS             complement(6705..7724)
                     /locus_tag="KR432_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0639820.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(7921..8181)
                     /gene="spoIIID"
                     /locus_tag="KR432_001273"
     CDS             complement(7921..8181)
                     /gene="spoIIID"
                     /locus_tag="KR432_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBH0639821.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(8279..8947)
                     /locus_tag="KR432_001274"
     CDS             complement(8279..8947)
                     /locus_tag="KR432_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBH0639822.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(9013..10077)
                     /gene="spoIID"
                     /locus_tag="KR432_001275"
     CDS             complement(9013..10077)
                     /gene="spoIID"
                     /locus_tag="KR432_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBH0639823.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(10164..11417)
                     /gene="murA"
                     /locus_tag="KR432_001276"
     CDS             complement(10164..11417)
                     /gene="murA"
                     /locus_tag="KR432_001276"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH0639824.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(11438..12175)
                     /locus_tag="KR432_001277"
     CDS             complement(11438..12175)
                     /locus_tag="KR432_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBH0639825.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(12659..13954)
                     /locus_tag="KR432_001278"
     CDS             complement(12659..13954)
                     /locus_tag="KR432_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBH0639826.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(14407..16239)
                     /gene="glmS"
                     /locus_tag="KR432_001279"
     CDS             complement(14407..16239)
                     /gene="glmS"
                     /locus_tag="KR432_001279"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH0639827.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(16506..17852)
                     /locus_tag="KR432_001280"
     CDS             complement(16506..17852)
                     /locus_tag="KR432_001280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH0639828.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(18022..18579)
                     /locus_tag="KR432_001281"
     CDS             complement(18022..18579)
                     /locus_tag="KR432_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0639829.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(18580..19332)
                     /locus_tag="KR432_001282"
     CDS             complement(18580..19332)
                     /locus_tag="KR432_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0639830.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(19332..20411)
                     /locus_tag="KR432_001283"
     CDS             complement(19332..20411)
                     /locus_tag="KR432_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0639831.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(20440..20655)
                     /locus_tag="KR432_001284"
     CDS             complement(20440..20655)
                     /locus_tag="KR432_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0639832.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(20698..21375)
                     /locus_tag="KR432_001285"
     CDS             complement(20698..21375)
                     /locus_tag="KR432_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0639833.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(21365..22444)
                     /gene="buk"
                     /locus_tag="KR432_001286"
     CDS             complement(21365..22444)
                     /gene="buk"
                     /locus_tag="KR432_001286"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0639834.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(22466..23368)
                     /gene="ptb"
                     /locus_tag="KR432_001287"
     CDS             complement(22466..23368)
                     /gene="ptb"
                     /locus_tag="KR432_001287"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBH0639835.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(23672..24853)
                     /locus_tag="KR432_001288"
     CDS             complement(23672..24853)
                     /locus_tag="KR432_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639836.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(24850..25689)
                     /locus_tag="KR432_001289"
     CDS             complement(24850..25689)
                     /locus_tag="KR432_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBH0639837.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
BASE COUNT         7741 a         5012 c         2837 g        10352 t
ORIGIN      
        1 tgttttcttt atggatttat cacaaattat gcaacttata ttctcaggat atataaaatc
       61 caaacattta ttaatcatat ttttaaaatt cttaataaaa ttatcattgc atattttcat
      121 atttattcta tcaacaaccc ttcttgttta aatttattta gcttatatcc taaatttgaa
      181 tacctttgat taactctatt attttttatc atatattcaa gatatttcac atctccaact
      241 aatacaacta atttttttgc tcttgttact gctgtatata ataaattacg gctaagtaac
      301 ataggaggag cccacgctat tggcaatact acaactggaa attcacttcc ctggctttta
      361 tgtattgttg tacaaaaact atggtcaatt tcatctaatt catcatatag gtatgataca
      421 attttagtct ggtcaaataa tatataaata gttttcttat ctttatctat gtgataaaca
      481 tatcctatat ctccattata gataccttct ccactttctt tttggtcttc tgtttcccat
      541 ttttttgtat aattattttt tacttgcatt accttatcgc caactctaaa taatctttta
      601 gaaaaacttt cttcaacttt aaatttttct tttttgttaa gatacttctg taactctata
      661 ttgagattag taactcccaa ttcacctttt ctcattgaag ataaaacttg tatatctttt
      721 aatttatcaa ctttataaaa tttaggtaat ctttcattaa ccaatcctat tatttcattt
      781 aaaatttctt cattagttga ttttcttata aaaaagaaat ctttaccttt tgtatttaga
      841 taaagtggtt ctccattgtt tatcttatgg gcatttacaa ttatcatact ttcttgcgct
      901 tgtctaaata tttcatttaa tttaactaca tttataatat tagaattaat catatcctta
      961 agtacatttc cagcacctac agaaggtaat tggtcactat ctccaactaa aattactctt
     1021 gttcctaact ttatagctct aagtaaattg tacattaaga taatgtctac cattgatacc
     1081 tcatcaacaa ttattacatc tgaatttatt gggtcttctt catccttaaa aaaagttaaa
     1141 tcatcatctg ttgaaaatcc catttctagc aacctatgta tagtttttgc ttctttatta
     1201 gaagtttcac tcattctctt tgctgctctt cccgttggag ctgccaaagt cacactttta
     1261 ccattatttt caaatatatc tatgatagca ttaatggtag ttgttttacc agttcctggt
     1321 ccaccagtta taattaatac tccactatta acagattctt tgactgctaa aatttgtttt
     1381 tctgctaact taattttctt atcttcttct aaaacattaa tctcgttatc tatatctatt
     1441 tttaagtctt caaattcata ctgtgataat tttactattt gactacatac tccattttca
     1501 gctaaatagt atggcataag gtatataaga ttttcattgt ttactttctc tatatgtatt
     1561 ttttgattat aaactagcat cattacacac tcttttatta tttctccatt tagctccaat
     1621 aatttttccg aatcttgtat tagaacatgc tctggtaaat atgtatgccc attacttaaa
     1681 gatttattta aagtgtataa gattccctga caaattctat ctttagagtt tttatctata
     1741 ccaattttac ttgctatact atcagctact ttaaatccaa ttcctcttat atcttcagca
     1801 agttggtatg ggtttttatt tataacttct attgcactgc ttttatattt tttatatatt
     1861 tttaggcagt aatttggtgt tattccaaaa ggagatagct gtattattat atttctcaat
     1921 tctctgtctt cttcataact ttttactatt tgttttactt ttttactccc tataccttct
     1981 acttcttgta atttttctgg tgaattttgt atcacctcta acgtatctac accaaactta
     2041 tcaattattc tttttgccat tttttcacca ataccatgta tcattccaga agataagtaa
     2101 acataaattc cttctaagga tgatggcgtt actggcataa aactattcac ttcaaattga
     2161 gtcccataaa tcttatgatt tacccacttt ccttcaactt ctatgctttc tccaattgcc
     2221 aaagtaggca tacatcctac aaccacaatt tcatcatttt catttaccag gtgagctatt
     2281 gtataaccat tatcttcgtt tttaaagact atttcactta tcattccttc taatttttcc
     2341 atttttatct cctaaaaata ctaataatcg ctccatttaa atgttagtat aattttatat
     2401 attaaaacta tgataatcgt agaaaagttt tatattttta tacttaacgt aatttccaac
     2461 tattatacat tttatcagaa acaaaaaaaa tctgcatcca taaaatatgg taccatgaaa
     2521 tacaggtacc atatttaaat agctatttta ttttaattac tctatattta gagtataaat
     2581 taaattccat aagtatatta cagtactaac ttatttcaga tttattctcc ttgtacccct
     2641 cagttttagt aattcttcta taaagcatac atagtaaaat taaaattccc atgtaacctg
     2701 tatatggata tagtgtacct actaacttat caaatggcag taaacctcct atacaagcta
     2761 atatacttac cactattgtt ataattttaa atttagggtg gtcatcttct acaaatcggt
     2821 tagttactga ccataacaat ggtacagctg tagtgtatat tcctagtaaa agtactactg
     2881 agaataaaat acctattatt ggagatatct tatcagctaa gtaaagtgct ggtatctctt
     2941 ttgtgtatat atttcctatg tttgaaagta atgccaaatt catcattata gctgctgcca
     3001 taagtgctac tccacctact ataccacccc atacagcatc tttcttattt actgcagaag
     3061 ctcccattcc tgtcaagaat gttattacta ttattacatt gtaacaagta taaagtactc
     3121 ccgagaaata tccatttgat gtagcagttt taatatttaa actatcaacc atatttccag
     3181 catttgataa agcatcaata ttgctaaata aactaattgc tcctactaaa agtgtaaata
     3241 ttattattat aggtcctata ttacctagta ttttagaaag ccttgtcaaa cctaatgaaa
     3301 cagttattag ggaaacgatt gccataccaa tcctacctac atatggattt aatccatagt
     3361 actctgatag tgtagctcct gctcctgcaa tcattattac taatgttcca aataaaaaca
     3421 atggaccaaa ccattcaaaa aatgttccaa gatattttcc acaatatact tgataaattt
     3481 ttattggctc cttaagtttc aattctcttc ccttatctat aacttctgct ccaaaccatg
     3541 aaaatagtac catagagatt attccaccta taatacccgc atatccataa aacgtaaaaa
     3601 attgcagtat ttcttgacca gttgcaaatc cagaacctat tacagtagca acataagctc
     3661 cagcaaaact tataaccgtt ttaatattta ctttgcctga catacactac tcccccccaa
     3721 ttaaaattat ttttaaagtg taagtttatt aagattatat tagactcatt attgtgatat
     3781 ttcaatatat ttttaattat ttaacattag ttaacacatt cttaaccatc ctttaacata
     3841 tatcccataa tatgtataaa tttagtagag tattatataa aatgtattga ccagattaaa
     3901 tactgtttat tcattatatg atgataaatt aacatttatt aaaaatattt aactcataat
     3961 aaaaaaactg ttagaaagta atcgtaaaat tacattctaa cagcttttta gttaaaaatt
     4021 attcacctaa agcatctttt cttaactgct caaccttgtc agttctttcc caaggtaaat
     4081 ctatatcagt tctaccaaag tgaccataag cagcaacctt tttataaaga ggctttctta
     4141 aatctaagtc tcttattatc gctcccggtc ttaaatcaaa gtgtttattt actaattcaa
     4201 caagtttttc ttctgaaacc ttgcctgtcc caaaagtatc tataaatata gataatggtc
     4261 tagctatacc tatagcatat gcaagttcta tctcacattt gtctgcaaga ccagccgcaa
     4321 caatattttt tgcaacatat ctagctgcat aagcagcaga tctatcaact tttgtagggt
     4381 cttttcctga gaaagctcct ccaccatgtc tagaatatcc accataagta tctattatta
     4441 tttttcttcc tgtaagacct gtatctcctt gtggtcctcc tataacgaat cttcctgttg
     4501 gatttatata aattttagtc tcttcatcaa gtaattctgc tggtataact tccttaataa
     4561 catgattaat taaatcttct cttattttat catttgatac tgtttcacaa tgttgagctg
     4621 atataagaac tgtatgtact cttacagctt tacttccttc atattcaaca gtaacttgag
     4681 tttttccatc tggtcttaaa taatcaacta aacctgtttt tctaacttct gtaagccttc
     4741 ttgataattt atgtgccaaa gatatcggaa gtggcattaa ttctggagtc tcattacatg
     4801 caaatccaaa cattatacct tggtctccag ctccaacttt ttctatttct tcttctatct
     4861 cttctccagt cttactttct agaccttcat caactcccat agctatatct ccagattgtt
     4921 catcaataga agttataact gcgcaagtat cacaatcaaa tccatatttt gctcttgtat
     4981 atccaatttc tcttactgtt tctcttacta atttaggaat gtctacataa gcagatgtac
     5041 ttatttctcc tgctactaaa actaaaccag tagtaactgt agtttcacaa gctactctag
     5101 attgtgggtc tttttctaat aatgcatcta atattgaatc tgatatttgg tcacatatct
     5161 tatcaggatg tccttctgta actgattctg acgtaaataa atgtcttgcc atttttattc
     5221 ccccttattt attcttaatt tttattatag gttttacctt atttttaatt ttagaaattt
     5281 actcaaaata aaaaaacctc tcctttataa gaagaggtta ataatcgtaa ttacatacta
     5341 attaactgac ctcatctttc aagacttttg tcttgtagga attagcacct tttcccattt
     5401 gggtggttgc tgggcttcac agggcctatc cctcagcctc tcttgataag gtaatcaaga
     5461 tttaagtttt gttttacttt actagcttac tattttttta aaattttgtc aatattttta
     5521 gtcatatttt ttctattcct ttttttgtaa gacaaataaa attttctatc tatctgaaat
     5581 ttaaagttag gtctttttaa tgctttttat tcatatattt ttttgaaggg ggtgttccta
     5641 ttgtatttag ctaaagtaaa ctacagcgat gatgatgtta tacagatgct aagttcagtt
     5701 ctaaaaacta taattaatga aaatacgatt gttgtttgca taggaacaga cagggctata
     5761 ggtgatactt taggcccttt agttggaaca atacttaaga acagtaactt taaatacccc
     5821 gtttacggta cactagataa ccctattcac gccttaaata tatatgagtc tttagatact
     5881 ataaaaaata cacatataca aggtaacttc ttggctatag atgcttgctt ggggtcgcag
     5941 agtaacatag gtaatataca gattagagaa ggacctatcc tgcccggaaa aggtgttggt
     6001 aaaaaactac ctcaaatagg aaattattct atagtaggaa ttgtagataa aattgatgaa
     6061 aataataaac tttcatttaa taatatacgc cttagcttta tattagattt agctgaaaca
     6121 atagccttgg ctttattagt atccacctaa tctaaatata aactaaatga taaaattctt
     6181 tatataaaaa ggtagatatt ttagagtatc tgattaaata ctccaaatta tctacctttt
     6241 tacttttata tttcaagttt ttatttttct actaaagcaa acattaattc acctttacag
     6301 acaagttctt cacctacata tgccttagca tctgccttag ctatattttt tctaagagtt
     6361 gtcatttcaa tttccatagt taatgtatct cctggtctta cttctctctt gattctaact
     6421 ttatctatac ctgcaaatac acctagtttt cctttgtttt cctctacaga catcattgcc
     6481 actccacata cttgtgctag agcttctact attagtactc ctggcatcaa aggatattct
     6541 ggaaagtgac cttggaaaaa tggttcatta actgttacat tctttatacc aacagctctt
     6601 ttcccaactt ctagctctgt aatcttgtct actaataaaa atggatatct atgtggtatt
     6661 aattttttta tttcatctat atttaacatt ctaaactctc ctaattattt tcttttgaaa
     6721 gacccaccag tttcaagtaa gtctattgaa cctaaagcta ttcctgtacc ttttgcaaca
     6781 caagataatg gttcatctgc tattgtaaca gttattcctg tcctctgttc tattatttta
     6841 tctaatccat ataataatga acctcctcca gtcattatta taccgacatc tcctatatct
     6901 gctgctaact caggaggcgt tttttccaaa acagcatgag tagcaactac tatttgctca
     6961 acacattctc taagtgcttc caacatttct gttgaattta tagttataga actaggaagt
     7021 cctgtaatta aatttcttcc tgttatcttc atatattttt cttcttctct cttaaatgca
     7081 gaacctattt caaacttgat tttctctgct gttctttctc ctataagaag attgtgttgt
     7141 tttttcatat atttaactat agcatcatca aatctatctc caccaacctt tatagattca
     7201 cttacaacag ctcctcctag agatattaca gctatatcta cagtacctcc accaatatct
     7261 ataatcatat taccattagg ctgtgatatg tctacaccag ctcctatggc agctgctatt
     7321 ggttcttcta tgatatatac ttctctagct ccagcttgtc tcgtagcatc ttctactgct
     7381 cttttttcca cttctgtgac acctgttggt acacatacca taattcttgg cataaaaaat
     7441 cttccaaatc cctttttatc aactatctta tctataaaag attttaacat cttctcagtt
     7501 acatcataat ctgaaattac accatctctt aaaggtctta tagcgactat attacctggt
     7561 gttctcccta tcatcttttt agcttcttct cccacagcta aaacagaatc tgttcttttg
     7621 tctatcgcca caacagatgg ttcttctaaa acgattccct taccactaac ataaacaagc
     7681 acatttgcag tacctaaatc tatgcctata tcagctccag ccattttttc aactccttta
     7741 tttataatat tagattatat tataagccta ttttaatact cctataataa agcatataca
     7801 aaaatctcgc tttaatcaag agaaaataaa tcttttgaga gtattattat tttgctattt
     7861 ataattactt aatatatttc tttttataat ttaattcaat aaaaaaagta ggtttttaac
     7921 ctacattttt ttatgttcct tctcatattt taatttcgtt gctaatcccc ctctaatatg
     7981 tctctctgat ttatttttgt ccagcacatt tttaacttct ttggcaaggg atggatttat
     8041 ttccttcaat ctttctgtaa catccttatg aattgtactt ttacttactc caaatgtctt
     8101 ggcggtttgt cttactgtag tgtttttttc aagtatatat tttgctacca ctatggccct
     8161 ttcctctata tgagatctca aagattaccc ccctcacata atagctttaa tgatatatat
     8221 gtaaaaacac caaaaaatat aacaagctta accaaatatg attaaacttg ttatactttt
     8281 acttaattag actcattggg tctatagatt tttctccact atacgcctct aaatgaacat
     8341 gtataccttc ttcactttct atttgagaag tactacctac agtacctaga gattgtcctt
     8401 ctgttacctt ctgttctttc ttaacactaa catttttatc tagatttgaa tatactacta
     8461 caatgttatt gtcacttttt attttgactg attgcccata ttcttcatca ttaaatacat
     8521 ctactactat tccgtttaat agagatttta cttctttgcc tttagtacaa ctaatatcta
     8581 cacctttatg agtttcccaa acatcaagag ttttagaata actaggttct ttttctgaat
     8641 atcctcttat tactttatct ccaatgtaat ttaattttgt tgtactcgat ttattttcct
     8701 cttttgactt agctttttct aagttttgtt ttgaatccgt tgcagtaggg atagcatctt
     8761 tcttgtcttt ttcaattaaa tgtatttcct cttcactatc tttattagca ttttccataa
     8821 ttcctttatt agaagccaat ttatctacat tattatttgt aaaccaaact ccacctactg
     8881 ctaataaaca aacacataca aacaaagata aataaaaacc atctttttct aacagctttt
     8941 tcttcatttt gcacctccaa atatatttct acatttggat tattgacgtg tttttggcat
     9001 tttatacaat tattagtata tatcttttat ttttgtatct gtgtagtaat gtgataatat
     9061 atcataatat ttatagccct cttctgccat tccttctgct ccccactggc tcataccaac
     9121 accatgacca tatcctttta ccacgaaatc tatgtatcct tctccaaatt ttatgtcaaa
     9181 gtttgcagag ttcaatttaa aaacagttct aatgtctttt ccagtcaatt ctttatttcc
     9241 taattttatt ttttctacag ttccggcatc acttctttta gttattgaaa cttgtttact
     9301 taaattattc acatctatca caatagtgct atatgccctt ctaagacttt taacaaaatc
     9361 tgtgttactt attttaagtg ttgacgcaaa tttaggggaa tacttatcat aaggactttc
     9421 cacagacttt aaataaggat attttgcaga aaatacttct tcactatttt ctgtttttcc
     9481 tgaagatgta gaaaaataaa gaggaagtat cgccttatca ttataagtta ttatttgacc
     9541 tttagttcct ctaactgctt cttggatttt ggaatattta ttcttaatcc attcttcacc
     9601 atttaatttt ttaagagtat cataactctt atattcttga caatgtttat aatcagtaca
     9661 tactactgca tttttatgtt tactagattt accatgttcc tgtttatata ctacatatgt
     9721 tctagctgct acagattgag cttttaaagc ttctatatca aactctgaag acatctcacc
     9781 agctagtact ccacataaat agttctccat gtccatcttt tcggttttcc ctgttatatg
     9841 attatataca ttgataattg gtgctttttt atttacagtt tcatagctca ctgtactttt
     9901 ttcttcttta ttacctttgt cttcaatatc acttttttta atagttttgt ttatagatac
     9961 tgggctctct ggtttttcag ttaattctac atttttataa gaaactaaag ttataagtga
    10021 tggaactaac acagagcaag tcacaaatcc taacaaaaca accaatgggt tcttcatata
    10081 ctctcctcct tagcttttaa aaccttgtat ttaagtatat gaacaaccct attaatttat
    10141 aactttcttc tataaaccaa ttattaatct tctactattt cgatttgacc acctagtgct
    10201 gttatcttct tatctatatc aacataacct ctttggatat ggtatatttc tcctatttga
    10261 gtttctcctt ctgctataag accgcataaa ataagggctg cccccgctct taaatcagta
    10321 gcgtttactg ctgcaccgtg tagctcatct acaccattaa caaccgcact tcttccatct
    10381 atttttatat tagcacccat tctattaaat tcagcaacat gcataaatct attttcaaaa
    10441 acagtttcta taactacacc agttccattg gctacagtaa gcatagccat aaattgtgct
    10501 tgaacatctg taggaaatcc tggatgtggt aaagttttta tatctattgg ttttaatact
    10561 tttggaccta caactcttac agaattgtcc atttctgtta tttcgcaacc agcttctctt
    10621 aatttagcta caactggttt taaatgctcc attaaaacat tttcaacagt tatatcacct
    10681 tttgtcatag ctgctgcaac catataagtt gctgcttcta ttctgtctgg aattacatta
    10741 tgttctgctc cttttaactc tttaacacct tttattttta tagtattagt acctgcacct
    10801 ttaacatctg ctcccatttc gttcaagaag tttgctaaat ctactatttc tggctcttct
    10861 gctgcattct ctattattgt agtcccttct gctaaagaag ctgccatcat tatgttttca
    10921 gttgctccaa cagatgggaa atctaaatat aatttatttc ctactaactt ttctgttgct
    10981 gcttcaacaa aaccatggtc catttctatc tttgcaccta aggctttaaa tccttttaaa
    11041 tgtaaatcta taggtcttgt tcctatagca catcctcctg gcatagaaat tttcgtacta
    11101 ttaaatctag caagcaatgg tcccataacc aaaaaagaag ctctcatctt tcttacaagc
    11161 tcatatggag cttcacaagt ttttatattg cttgcatcaa cagtaagtgt attttcttta
    11221 tattcaactt ctgctcctac atgtcttaat aaatctgata taacatgaac atctcttaaa
    11281 tttggtactc catttaatgt tgattttccg tttgctaata aagtcgctgc tattattggt
    11341 aaaactgcat tttttgcacc atctatttta acactacctt taagtggatt actctttttt
    11401 actattatct tagccatatt ttttgatctc ctcataatta ataatctaat tttataattg
    11461 gtgtggcaat atatatgagt gtttttccgt catcctcatt atatttaatt cctatattta
    11521 aattaatttt ttcccctaaa tattctaatt cataatcatt taatacttta ctatatgctg
    11581 ttatagataa aaagtttacg tctttaactt catctatttg cttagcactc atattatata
    11641 atattttttg taaaatatca tcatatttgt tgttttgtaa cctttttgtg tattctccaa
    11701 caatacaagc agacacatct acttgagatg aatatttatt taacacattt tctacgttac
    11761 tataaataac ctctttatgt ttatatactt tgttatccaa tatgtcaact actatgtaac
    11821 tttctttgtt atttttattt attcctttta tagaaatttc tttatcgcta ccttttatag
    11881 tagcacataa ttgtatttta tcgccagacc ctttttgatt ccaatttatt ccttttttat
    11941 ccaactttag attgttaatt atttctataa aaatattatg aaattcttta tttgataaat
    12001 tatgatttat aacagaatta attttaacat tataaaattt aaaatttgct tctgatattt
    12061 caagagcttt acttaatcta ttgtatctag tatttatact tgtatctgca taagatattg
    12121 tcattcctat aatcattaat ataaaaaagc ttatagctaa ttttactttt ttcatagtaa
    12181 aaaatccccc ttaaatatgt ttttctgtat aatagaatta ttaacaaatt taagagagat
    12241 atactttctc aatatgtatt tttatacatt tttgtatttt tcatctaata atctctttca
    12301 aactcttcaa tctcaatttt ctctatcctt ctcctggact tgccaattcc ttatatgtat
    12361 caaatacatc ttgcattaat tcttatatat tatcttctgt aatataaatt acttagtcta
    12421 tacttaaatc tataatctat aatctataat ctataatcta taatctataa tctataatct
    12481 ataatctata atctattcat attttaccag caaaataaac agataatact tttttatcaa
    12541 tttccatagc tccaaatgaa catacctaag tacacttccc aatacacaat ttacacactc
    12601 ttcctaataa atcaaaaaca cctttgtttt aaacaactct attgtacttt caaaaacttt
    12661 atttgtgttc tcccacttca attaaatttg aattatcaag actccagccc atccatccat
    12721 cactataaag ggaggtattt tccactccta tgacatttgc atatgttaat acttcagcag
    12781 cacgccatcc acttccacac ataaatataa gctgtttatt cacatctata ttttccttgt
    12841 cccacatttc taaaatctca gatttatttc tcattgtatt atcaatatta cgatattctt
    12901 ctagtgaaac actattacta cctgaatgtc catataaagc tcctggtata cgcccttttt
    12961 tatcataata tgtatatcca gatacttttc ctatatgttc atcccaagta cgattatcta
    13021 ctagtataaa cttatttttt tcttttagtt tttgtcttaa ttcagatgta gtaacaacta
    13081 attgtgagtt aactggtata tctgctccaa aattagtaca tgaatgtttt ggattactaa
    13141 taaattctaa ttcatatcct gctgataacc atgagtttgt tccaccattc aaaactctta
    13201 catcttttac tcctatatat ctcaatataa cagctagtct ataagatgcc ataggtgtaa
    13261 tacttgaaac tattacagta tcatcctttg ttagtccata gtctaaagca aacttagtta
    13321 agttatcatc attatcaagc atccatgttg gaggaggttc tataatatca gtatttacat
    13381 gaacacttgt aggtatatgc ccttttgtat acgattcttc tccccaactc gcttcaatca
    13441 tttttatatt ctttgaatct tcaaatgtct caggaatttt tccatctaat atatctttta
    13501 ttataaaagc aggaactatc atttgataat ttttatatct ttccattgga agattttcat
    13561 cgtctgccca ttgtttaata tcatatttat atagatattt atatcctttt ttagataaat
    13621 aatctgctac aaccaaagca tcttttccat ttgcatcata caatactata tttttatcta
    13681 aatcaattct tttagttttc aatgcttgtt ctagcacttc atcttttcta tctgagtata
    13741 catttaacca attagcagaa aaatctactg ctctttttat atgcccacct ctttttactc
    13801 catctaactt ccagccatta taagcatcat ttaagcgagt atcaactata acccaagcgt
    13861 tatcttgcat tttttcttgt aaaacttcag tagatattgc cttaagatta acaaaataat
    13921 cttctttctt ttctttttta aaatcatttg tcataattta tactcccaat ttaaaaaaat
    13981 ttttatttga aattatttta ctaaaataaa gtaattttct tatactctat tagcttcatt
    14041 atattctttt ataaatagtt ccttatattc ttattttaaa ctactcactt tatcttatca
    14101 caaggttact aataccttgt attttaattt ttctcataag ctattatttt tttatgtctg
    14161 atattgacta tatgtttatt aagtactcac tatattttat atgaactata tttatcgttc
    14221 cctatacctg gtatggtcta tatatttatc attttacaga tgatttaata tcaatctttt
    14281 tataaaattt attatagcaa aaacaaaaca aatttaatat ttatctaaaa aactcatcct
    14341 ataaaataca aataacagga tgagtttttt tcaataaata ttatctattt aatttttaat
    14401 ctgtttttat tcaactgtta ctgatttagc tagatttctt ggcttatcta catcgcatcc
    14461 tcttagtaca gagacatgat acgcaagtaa ttgcattggt acaactgcta atattggtga
    14521 taaaatatca tctgaatttg gtatatagat tattttatca gcagctttct caacatcctt
    14581 attatggctt tgagctattg ctactacata agctcctctt gctcttactt cttccatatt
    14641 agaaaccatt ttttcaaata acttttcttg agtagctatg gcaattacag gagttccttt
    14701 ttctattaaa gctattgttc catgctttag ctctcctgct gcaaaagctt ctgcatgtat
    14761 atatgaaatc tcttttaatt tcaatgaacc ttccattgct aagctatagt ctatacctct
    14821 tcctaaatag aatgcatgtt cagaactaac aactgtttta gcaacttctt ttatatattc
    14881 ttcattgtct aatatttctt gtatttttga tggtatttct ttcatcttag aaatcatact
    14941 atcataaaat tctctagtta ttgttccttt tttgatagca aagtctaaag ctatcatgta
    15001 taaagaagtt atttgagtag tatatgcctt agttgatgca actgctactt caggtcctgc
    15061 ccaagtatag aatacatcat ctgattctct tgctatagat gaacctacaa cgttagttat
    15121 tgaaagtatt cttgctcctt tagcctttga atctcttaat actgctagag tatctgctgt
    15181 ctcaccagat tggctaacta atattactaa tgaattttca tctacaaatg ggtcgctata
    15241 tctaaattct gatgctatat cagttattac tggtatattt acaaattttt ctatggcata
    15301 cttaccaagt aatccagcat tatatgctgt tccacaagcc actatataaa ctttatttat
    15361 tttttctaag tcttctttag aaatatttat actgtctaga attatatttc cattatcatc
    15421 aagtcttctt tctaaagttt ctctaactcc tgttggttgc tcatgaattt cttttgacat
    15481 aaagtagtca tatccacctt tagacgcagc ttctacatcc caagttacat gaaatacttc
    15541 tttttcaaca agatttctat tagagtcata aactgttaca ttttcgtctt ttagatgaac
    15601 tacttctcca ttttctaaga aataaacatc tctagtatat tttaataatg caggtatatc
    15661 tgatgctata aagttttcac cttcaccaac accaactaca agaggactat cttttcttac
    15721 tgcaactaat tcatttccat gttctttaca tataacacct aatgcataag ctcctcttaa
    15781 ttttgatata gttttgtata cagcatctaa taaattaccc tcatagtatt tatctactaa
    15841 atgtgctatt acctctgtat ctgtttgaga ttcaaactta acaccttcag aaattaattc
    15901 ttcttttatt tccatatagt tttctattat cccattatga acaactgcta ttgttttggc
    15961 ttgattaaaa tgaggatgtg agtttacatc agatggttct ccatgagttg cccatctcgt
    16021 atgccctata cctaaatttc catcaatagg attcttttct aagtcttctg caagtacaga
    16081 aagtctacct ttaaactttc taatattaag ttcttcttca ttagaactat taactgctac
    16141 tcctgctgag tcataacctc tgtactctag cttagaaagc ccttctacta taacttctgc
    16201 tgcctttcta cttcctaaat atccaactat tccacacatt atttgtgtcc tcctaaaaat
    16261 attatttata tattttaaga gttaggtcat ataagtttac tttggcttcc ctttgtccat
    16321 aggattgctc ctattattta aagaagtctt tcgatggtaa acacaccgca gagcatccgc
    16381 cgacaattcg attaactctg acctcgtcaa cttagttcct atattactca ataattatgt
    16441 ctataactaa gttctggcgc ttagaaaggt gtcctcagta tttgtatctg agaataccct
    16501 ttaaattacg ataatttttc ttgtataaga ttagctaagt ttgttgctaa ttcttttatt
    16561 tgaccttcct ctttaccctc taacataact ctaactaatg gctcagttcc tgatggtctt
    16621 atcaaaactc taccatttcc gtttaaaata gattctattc tttctatctc agtttttatt
    16681 tctggatatt ccatatattt gttcttattt tcatttttta ttgttgcatt tactaatact
    16741 tgtgggtatt ggctcattat tgatgcaatt tctgataaag gtttcttttc ttgcaagatt
    16801 atatttgcaa gtattagaga actaagcact ccatctccag tagtgttgta atctaaaaat
    16861 atcatatgtc cagattgctc tccaccaaga ttatatccat ttttaaccat atcttctaaa
    16921 acatatctgt ctccaactgc tgtagtagat aaatttattc cattttcctt agcagctatt
    16981 gtcaacccta tattactcat aactgtaact accaaagtat cttgcgccaa tttattattt
    17041 ttctttaaat ttaaggcact caatatcatt atatggtctc catcaacaat ttgacccttt
    17101 tcatttactg caattaatct atcagcatct ccatcatatg caagacctaa gtcagcatta
    17161 tgttccaaaa cagccctttg aagttgttct ggatgtgtag agccacattt atagtttata
    17221 ttatttccat ctggtgaact atttattgaa attacacttg ctcctagttc atcaaacact
    17281 attggtgcca ctttatatga cgcaccatta gcacagtcca aaactacttt taatccctta
    17341 aagtcagtgc ttattattga ttttaggtag tctacatagt ctctttgtgc acaatgttca
    17401 tgtaattttc ttcctacatt ttcaccaatt ggaagacaat caattttgtc tatatcgtct
    17461 atatattctt ctattttcaa ttcaatttca tcatctaatt tatatccatt cttattaaag
    17521 aatttgattc cattatattc aacaggattg tgagatgcag atataaccac accacaatca
    17581 gcaccatatt ttctagttaa atatgccact gcaggtgttg gtattattcc aacagtaata
    17641 acatcacatc caacagacat aagtcctgct attaaagatg cttccaacat atcacctgat
    17701 attctagtat ccttacctac tactactttt actctgtggt ctccttgagc taagacaaat
    17761 ccaccagctc taccaagttt atatgctaaa tcacatgtta gttctgtatt tgctactcct
    17821 ctaactccat ctgttccaaa atactttctc actaaagatt ctcctttcat aattgaaaag
    17881 cttgtaattt gtattatatt tttatagttc ataaaatatc attataggct taatttcagt
    17941 cataaaaaca ttttaatact tatcagcaaa aaagacaata gaaataattc tattgtcttt
    18001 tttttgacat ctttttcttt attagttagt tgctttagaa catgtagcat ccataccttt
    18061 ttgtaaagct tctctgttta atggaacgaa tttttctttt ctaggtccga aaactttttt
    18121 gaatgcttct agtacagatt ctatatctac agtatcagat acttttaagt aagcgcctaa
    18181 cattatcata ttagcaactt tatcatttcc taattcagct gctaattcat ttgctggtat
    18241 ataataaaca tctacatctg ttctatcaac tttttctttt attaaagaac tatttactat
    18301 tatttttcca ccaggaataa catcatcttt gaatttttca aatgctggta tatttaataa
    18361 tatagctgca gttgcatcat ctgttataag tggagaacca actggctcat tagatacagt
    18421 tactgcacag tttgctgtac ctccacgcat ttctggtcca taagatggta accaagaaac
    18481 ttcttttcct tctagcattc ctgcataagt aagtaattgt cccatagaca taacaccttg
    18541 gcctccgaag cctgcacata ttactctagc tgtagacatt tacttcacct cctcaacttc
    18601 aacatttttg aaatttccta atggatagta tggtatcatg ttgtctctta accattgtaa
    18661 agcatcgttt ggagctagtc cccagtttgt aggacaagta gataatactt ctactattcc
    18721 aaatcctaat cctgcttgtt gaacttggaa tgctttcttt atagcttttt tagcttgtct
    18781 aacatgagct ggagtatcaa ctgctactct ttctacaaat acagctcctg ttaatgtagc
    18841 taacatttca cttactctta ttggataacc ttgagtttca gcatttcttc ctgattgagc
    18901 agtagtagct ctttgtccaa ctaatgtagt tggagccatt tgtccaccag tcataccata
    18961 agttgtattg tttacaaata tagtagttat tttttctcct ctagcagctg catgaactat
    19021 ttcagcagta ccaattgaag ctaagtctcc atcaccttga taagtgaata ctgtattttc
    19081 aggatgaact ctttttattc ctgtagcagc agctggcgct ctaccatgag cagcttcttg
    19141 agtgtcacag ttaaaatatt tatatcctaa aactgaacat ccaactggaa caacacctac
    19201 agcatcacct aatactccta gttcttctaa tacttcacca actaatctat ggattatacc
    19261 atgtgtacat cctggacagt agtgagtttg agtatcttgt aatccttcag ttttcttaaa
    19321 tacaacagcc attatctagc acctcctgca accaattctc cggcaataat ttttttagct
    19381 ttttcaacta tttctgctgg tgttggagtc attcctcctg gtcttccatg gaagtaaact
    19441 ggtaatttac cttctactgc cattttaaca tcttccacca tttgtcccat actcatttca
    19501 acagtcaata tgtttctagc attaggaatt tggttgaatg cttcgaatgg gaatggccat
    19561 aatactttag gtcttataag acctgctttt attccttctt ctcttaatat atcaattgcg
    19621 ctttttacta ctcttgaagt agttccataa gctgcaaata caaattcagc atcttctgtt
    19681 ttgtacattt cgtattgtac ttcattcttt tccattgcat caaatttttc ttgtaaatgc
    19741 caacaatggt cttctagtac ttctggttct aagtatagag agtttattac attaggtttt
    19801 ctttttcctt tagttccaac agttgcccaa tctttaggag gcaattctct tttcttctca
    19861 ggagctctaa attcaactgg ctccatcatt tgacctatca taccatctgc aactaccata
    19921 actggtgatc tgtaataatc tgcaacgtca aaagcttcca ttatcatatc aacagcttct
    19981 tgaactgtag ctggtgcgaa tactggagtt ctgtagtctc catttcctcc acctcttgta
    20041 gacataaagt agtcagcttg tgaaggttgt atacttccaa gtcctggacc acctctcatt
    20101 acattaagta caacacaagg aacttctgct cctactgcat atgttatacc ttcttgcttt
    20161 aaagcaactc ctggtgaaga tgaagaagtc ataactcttg ctcctgcacc tgctccacca
    20221 taaaccatgt ttatcgcaga aacttctgat tcagcttgaa cgaacgctcc acctatttta
    20281 ggtaactctc ttgataaata ttctggtaat tcactttgtg gagttattgg gtaaccgaag
    20341 aaatatttgc aaccagcttc tatagctgct tttccaaatg cttcgttacc tttcataagt
    20401 atcttagcca ttaaaattcc cccttaaata ttaattaaat taatctcttt ctactgttat
    20461 aactacatct ggacacatag ttgcacaact tgcacatcct atgcattttt ccatttcaaa
    20521 aacagttgct gggttatacc cttttttgtt tattacattt gagtctaatt gtatgatttt
    20581 tactggacat gcttcaacac acaatccaca acctttacaa cgttcttgat taaaagatac
    20641 ttttccttta gccatttacc taagcctcct taaactttct atatatttga gctcaaacta
    20701 catccagtct tctctcatat ataattttat aggaaaaatt tctccttcta aatcttttgg
    20761 taactgttca acaagatttt ctaaacatgc aacatatctt atcggtatat tacatttttc
    20821 agaaacttct tttgcaactt tctgacctct taaaacatca tcaatagtag taaacctaat
    20881 aagatgagta ttattaatta gacctgttac ctttaattta gaagattttt ctatttcttt
    20941 tatatattgg ataacttctt cagaagtttg agttttctcc ctatttgcat ttattacaaa
    21001 aagcatatca taatcgcctt cttttaacaa atgactaaat cttcctataa ccctagcacc
    21061 tacattatct ccacctaaat ctataacaga attgtatgaa tggtctacca taggagacat
    21121 tacctctgct gatactgctg gtaaatctaa tgttggagca ttaattgagc tatctattgg
    21181 ttgtatacct aaggatttca ttaactcttt cttttctcta gttctaaagt atacatttac
    21241 cacatctaaa tcggctatag caactttgcc agaaaccata tctcttaact taacaacata
    21301 gttcatcgca aattcacttt taccacttcc atagtgacca gttatgattc ttattctttt
    21361 atcattagtt atcataaact tgagcctctt cttcaccagt taaaactcta agtccacctt
    21421 gagctaatgc aatcatttca tcttcacctg gataaacttt tacatctgct ataaatttaa
    21481 ctctatctgc aatcatttct gtaaacattt ttgaatatgc gattccacca gttaataata
    21541 ttgcttttac atctccctta agaactgcag cactagctcc tatttcttta gagatttgat
    21601 atgccatagc ttcatatact aatttagctt tttcatcgcc agcttcaatt ctttcttcaa
    21661 cttctctagc atcattagtg tttaagtatg caactaatcc gccattacct tttattttct
    21721 ttttaatttc atcttgagta tattttccac taaagcacat ttttactaat gcacctactg
    21781 gtagtccacc acttctttct ggagagaaag gtccttctcc atctaatgcg tttgcaacat
    21841 ctactatttt accattttta tgagctccaa cagaaactcc tccacccatg tgtgcaacta
    21901 taagatttat atcttcatat ttcttgttta tttctctagc atatcttctt gctattgcct
    21961 tttgatttaa agcatgtact acacttgctc tacttatttc aggcatacca gaaattctag
    22021 caacatcttc taattcatct acaacaacag ggtctactat gtatgaagga acatttactt
    22081 cttcacctat ttgttttgct attattccac ctagatttga agcgtgttct cctaaaactc
    22141 ccacttttaa atcttcaatc atagcagcac ttactgaata agtaccacct tttataggtt
    22201 taagaagtcc tcctctacct actacagcat ctaattcaga tgtttttact ccaccttctt
    22261 ttagagcttc ttctattact tgtttacgaa attcaaattg gtcagacacc ttctcatatt
    22321 ttcctatttc ttctgaagaa tgtcttaaag ttttttcaaa tactaaatcc tcattatcaa
    22381 atacagctat tttagttgat gtcgaaccag gatttattgt taagatttta aatattttgc
    22441 tcatttgcct atttccccct taaaattatg cctttgctgc cattaaaaca cctaaagcta
    22501 ttgagtttag tttagtttct tcactgtctg ctctagaagt taatattatt ggtgctttag
    22561 ccccaactat aactcctgca ttttttgatt ttgagaagaa tactaaagct ttatataata
    22621 tgttaccacc ttcaatatct ggggctaata atatatcagc tcttcctgct acaggatgat
    22681 ttataccttt atgtttagct gcttctaaag atactgcatt atcaattgca aaaggtccac
    22741 caaccataca acctttgatt tctcctcttt catacatttc ttctagttct ttagcttcaa
    22801 ctgtatcttt catttttgga tttacttttt cttttgcgca tattgcagca acttttggtt
    22861 cacttatatc taatgaatgt gctactgtgc aagcattttc tatgatttgc tttttagtat
    22921 ttgtatcagg agctaagttc atagctgcgt cagttacgaa aaataatcta tcatatccct
    22981 ctacatcaaa tactgctacg tgacttaata catttccagt tctaagacct acttctttat
    23041 ttaaaactgc ttttagtatt attgatgtgt ctactaagcc tttcattacc atgtcggctt
    23101 ttccttgtga aactaattca acagatttta gagatgcttc tgctaaatct tttatatcta
    23161 tcagttcata attttcgata tccatgtcta tgctttttgc aatttcttta gtcttttcta
    23221 tatctcctac taaaatggca tttgctattt tttcttttct agccatttca actgccatta
    23281 aaacttcttt atcttggcaa catgctactg atatagtttt aggtcctctt tcttttgcaa
    23341 acttaattac ttcttcaaaa cttctcatgc atttccacct catcttgaat tttttgaaat
    23401 agaacttttt atatctcaat taaattataa ctcgttcagc aacgttttcc tagagataat
    23461 tgaaatattt aattcaaaat atttatttaa atctatttat cagtcttttt tcatatatct
    23521 agcgcttttt agatttaaca aatatctcat tgtccttatt ttataatata actaaaataa
    23581 acaagatgca acaaaaacat aaaattaaca aacttaattt tataggtttt tttgtcgcat
    23641 cttgtaacaa ctaaatattc gaaatatttg ttcattccac ttcaacaata ttaggttcta
    23701 tatttatttt tttaaattga tatgttgttt catattttat tttatattta ctatcttcct
    23761 cagggccttg tgataaatca atatataatg aaatatcttt tttatttaac ttactcaaat
    23821 cctccgttga ctctacaaca acttttatat ctacatcaga aattacattt ggattatcta
    23881 tatttgttgg gatatttctt aattcaatat ctcccgcatt atataaaaat tctgcactca
    23941 attgttttgt atcatttaat ttaattgtta tagagctatc ctcaagagtt ataccctctg
    24001 gaatttctaa gctaacttct ttttcttcac cattagccaa atcagctaag tcaataggtt
    24061 ttgtcttaat ataacttatt ttgtctatat cttctttctt tccttttatt gtaacttgtt
    24121 ccttacttaa tttatagttt ttcagtacat cattatcttt attatcattt agacttactc
    24181 ttattgggac aattttttcc ttcaatagtt ctatctttgc agtcacaaag gatgaactta
    24241 atgttactcc ttcaacctta gttccatcac tatttaatgg tattaattgc aattttttag
    24301 aaaaatcatc tgtctcatta ccaacatcta gattagctct tacacttttg acttctttaa
    24361 ctagagttct tggacctgat acatcgacaa attccttact aagttcaatt ttatttatat
    24421 ttctttttga atttccactt acttctacct ttatatctct tttttctttt atgttttttt
    24481 caagatttac tactaaaaca tcatgcttaa attctagagt aacaccttga ggaatatttg
    24541 cttttaaata tacttccttc tgtccttgta ttgggtcttc aatggttcca tatacatgta
    24601 tatcttcttt cttaacactt tgtacctttg ataaactacc ttttatatat acatctgctg
    24661 ttaatttttc gtctggatat ataacaagat ttttatcctt taaatcactc atattactta
    24721 tagatacagg tatatcttca aaccctttag tttcttcagg gtcaactaca gccataacat
    24781 acatccataa tgcaattgca cttaaaaatg atattatttt tattttggta ttatttttta
    24841 gtctatttat catttaaata taccaccttt gaaaaaactt cttgtttctg tatttgtttt
    24901 taaattacta cgtaatatat tcatcattct ttctctagaa atatctctat ataacttacc
    24961 tgctttagca attgatacgc cacctgtttc ctctgataca atcagagtta tacagtcaga
    25021 cacctcactt acccctattc ctgctctatg tctagtaccc aaatctttac ttaaatcctt
    25081 actttccgtc aatggcaaaa aacatgcagc tgcttttacc ttagaatctc taattacaac
    25141 agctccatcg tgtaatggtg tatttggtat aaatatattt attaatagtt gcctagaaat
    25201 ttcagcatca ataatagttc ctgtatttat tatatcactt attctagttt ctctctccat
    25261 tatgataaga gctcctattt tttgtctaga aagtgaatat aaagcttcta caatttcttc
    25321 tatatttcta ttgagagttt cttcagaagt atttacattc tttccaaata aattaaactt
    25381 tgctcttcct atatgttcaa gtcctgctct aaattctggt tgaaaaatta taagagcagc
    25441 tataactcca taatctaaag ctttaagaga tatccaataa acggtatgca atttaaaggt
    25501 gttactaagt tgagttgcta ataaaagaaa tattatacct ttaaacactt gttctgctct
    25561 tgtatctttt ataaacataa atattttgta aaaaatatac gctactatag atatgtcaat
    25621 taaatcatat atactcattc ttaatattat attaaaaaat ccatttaaaa aagaatttat
    25681 atctaacaca gttttatcct cctgtttgat aacttcttaa ttatttacac taagtattat
    25741 actatataaa tctaactcta tacaaactat ttaactttta tgtaaataat tctagtttta
    25801 tatttggaga ttgttaaata atctctaaac acattaaaaa tatcaactta ataaatacat
    25861 atttcttatt aaagttagtt tttactcaaa taactcaaac catcaaggcg cgaatacaaa
    25921 atagtactcg cgccttaatg ca
//
LOCUS       DAEPOG010000036        25921 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEPOG010000036 DAEPOG010000000
VERSION     DAEPOG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.036"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            317..772
                     /gene="smpB"
                     /locus_tag="KR432_001290"
     CDS             317..772
                     /gene="smpB"
                     /locus_tag="KR432_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH0639838.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            930..1279
                     /gene="ssrA"
                     /locus_tag="KR432_001291"
     tmRNA           930..1279
                     /gene="ssrA"
                     /locus_tag="KR432_001291"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1953..2858)
                     /locus_tag="KR432_001292"
     CDS             complement(1953..2858)
                     /locus_tag="KR432_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBH0639839.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            3769..3984
                     /locus_tag="KR432_001293"
     CDS             3769..3984
                     /locus_tag="KR432_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBH0639840.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            3984..4463
                     /locus_tag="KR432_001294"
     CDS             3984..4463
                     /locus_tag="KR432_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBH0639841.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            4469..4699
                     /locus_tag="KR432_001295"
     CDS             4469..4699
                     /locus_tag="KR432_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639842.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(4815..5981)
                     /locus_tag="KR432_001296"
     CDS             complement(4815..5981)
                     /locus_tag="KR432_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0639843.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(5971..6723)
                     /locus_tag="KR432_001297"
     CDS             complement(5971..6723)
                     /locus_tag="KR432_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0639844.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(6815..7306)
                     /locus_tag="KR432_001298"
     CDS             complement(6815..7306)
                     /locus_tag="KR432_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639845.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(7557..8321)
                     /locus_tag="KR432_001299"
     CDS             complement(7557..8321)
                     /locus_tag="KR432_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639846.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            8468..8668
                     /locus_tag="KR432_001300"
     CDS             8468..8668
                     /locus_tag="KR432_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0639847.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            8672..8986
                     /locus_tag="KR432_001301"
     CDS             8672..8986
                     /locus_tag="KR432_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639848.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            9008..9751
                     /locus_tag="KR432_001302"
     CDS             9008..9751
                     /locus_tag="KR432_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639849.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            9764..11098
                     /locus_tag="KR432_001303"
     CDS             9764..11098
                     /locus_tag="KR432_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0639850.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            11348..11620
                     /locus_tag="KR432_001304"
     CDS             11348..11620
                     /locus_tag="KR432_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639851.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            11893..13020
                     /locus_tag="KR432_001305"
     CDS             11893..13020
                     /locus_tag="KR432_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH0639852.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            13500..14147
                     /gene="cas5"
                     /locus_tag="KR432_001306"
     CDS             13500..14147
                     /gene="cas5"
                     /locus_tag="KR432_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0639853.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            14138..14902
                     /gene="cas6"
                     /locus_tag="KR432_001307"
     CDS             14138..14902
                     /gene="cas6"
                     /locus_tag="KR432_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0639854.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            14904..16070
                     /locus_tag="KR432_001308"
     CDS             14904..16070
                     /locus_tag="KR432_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639855.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            16091..17161
                     /locus_tag="KR432_001309"
     CDS             16091..17161
                     /locus_tag="KR432_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBH0639856.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            17172..19157
                     /locus_tag="KR432_001310"
     CDS             17172..19157
                     /locus_tag="KR432_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0639857.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   19410..20032
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19410..19438
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            20477..22033
                     /locus_tag="KR432_001311"
                     /pseudo
     CDS             20477..22033
                     /locus_tag="KR432_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            23211..24425
                     /locus_tag="KR432_001312"
     CDS             23211..24425
                     /locus_tag="KR432_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HBH0639858.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            24425..25717
                     /locus_tag="KR432_001313"
     CDS             24425..25717
                     /locus_tag="KR432_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0639859.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
BASE COUNT        10502 a         2612 c         3978 g         8829 t
ORIGIN      
        1 tagaagatag aagatagaag atagaagata gaaaatttat aatatgtaat atttttgttt
       61 tgaaatttag aaatcagtgt tactggttaa agttagttta gtattaagta tattcgaagt
      121 agttttttag ctattttcat atctttatat tacttaatta tatatagcct aactaactta
      181 atatatcagt gaaatattga ctaaaatgcc taaatatgtt atcatacaag ttagtcaaat
      241 aatttggcaa cattaaatat ttagtaaatt tctaacattt atggaataaa tttctaacaa
      301 agaaggtgag atttatatgg ctggtaaaaa aatattagca acaaacaaaa gagcaagaca
      361 tgaatatttt atagaagaaa cttatgaatg tggaattgaa ttaaaaggaa cagaagtaaa
      421 atcaataaga caaggcaaag ttaacctaag tgatggattt gcatctgtag ataatagtga
      481 agtattttta aaacaagttc atataagtcc atatgagcaa ggaaatatat tcaatgttga
      541 ttctttaaga gttagaaaac tattacttca taagcatgaa ataagaaaac ttattggagc
      601 aactactata aaggggtatt cacttatacc acttagtttg tatctaaaaa acggtaaagt
      661 taaagtagag attgccttag caaaaggtaa aaaactatat gataaacgtc aagatatggc
      721 taagaaagat gcacaaagac gtatagaaag agaaatgaca ggtaagtatt aaactagata
      781 aaactgtatt ttttagaata aaattcttta ttatctagaa cataacattg atttttattc
      841 ttaaaagtat tatattatat atacattaaa aatttaataa aaatggtgat aaagtttgcg
      901 aaaagtctac tggactataa actactcaag ggggcgtaaa ggtttcgacg tgggtttgga
      961 acttgaggct gcatgtcgtg ttactctggg tcacgtaaaa actggggaac tttaaaataa
     1021 acgcagacga taatttcgcg atagcagcct agttgctgct cgtccctctt agtcctcctg
     1081 ccgactaaga ctggacgtca ttatgcaggg aactacttta ggggtgtctc gacctgaagt
     1141 agattaattg ggactggcta agcatagagc ctgccacttg gcgctatgtg aggtgagata
     1201 ttagtgaagt tggctaagca tgtagatgca gcaaggaaaa gatttgcgga caggggttcg
     1261 actcccctcg tctccaccaa tttttaaaaa agaatgatga aatacttgga aatactagtc
     1321 tttattgact agtgtttttt gttttggaga tgaaatccac caaccgtgcc ccaaccacag
     1381 aaaaagagat ttttttgggg tatattgggg tgcatttgat taatccatgg aacatataaa
     1441 gaatactttt agagatatag tgaatttgat gataatacaa taaatatttt atactgatga
     1501 attgttttta tattatgctt tttatttcaa tctaggaaat atataacttg tttaaactta
     1561 tgattaaaag gataaagaag tttaaagcat ataagtgcta cttaatctat tagagtaatt
     1621 actagttata atgatataca gaatcctata agattatgta taagtataga acaaatttat
     1681 tatctatgtg ggatgctaat ttaaattttt taattgtatt taaaaaaata agatgtaaaa
     1741 aaggaattga aaaatgttaa gataataaac acatagaatt attaagttta atactaacaa
     1801 gactattgta tatagacaat atattatata taaaatttaa ataatatatc ataaaagtac
     1861 ctatactaat aagtactttt atgatataag ttaattctat ttttataata taaattaaaa
     1921 gtatttaaag cgttgttaaa ggctatagtc tattattcat catattcgat ttcgagctgt
     1981 acaggtagat tgaaagctct attaaaaagc acattgaaat ctttccagtc ttttgatgct
     2041 tgcattaagg ttataacctt taataaatga ttttttaaat gatcattacc tatatcttct
     2101 gttaaaaatt gatgatgttt atgttttctt tgcccaggtt ttactgtggg gttaactctt
     2161 tgaagttctt ctttaactcc aggaggtaat aaatcataaa catatttatt tgtgtatgtt
     2221 ccaactattc caggtctctt agaacttggt ctagggtatg tccagttttt taatctgaac
     2281 atttgtctat aaaattcatc aggaaatctt tttgtccaag gtaaaaactc tttagaaata
     2341 tacatagaca atattttttg cagctcatca cgatctctat catattgata tcctgtagct
     2401 tcatcaacta atgctacaat ccctattttg gataaacttc ttactaaaat ttcgcttgct
     2461 atagctagtc cttcttgctg aacagctaat ttattatcaa gccttgcttg taaatataca
     2521 tcacatataa gaggtattat ttcagctgga taaccttcca caattcttcc gtttttagtc
     2581 ttatatttta ttgtatttat aagcatatct tctaattcat ctgaaatata atcagataga
     2641 ttctttgcat caataaaact tggtagatta ggcttagaag gtacacgaat ttcatctttt
     2701 tttctaccac gctttgttct tccaaaagct ttgaatactg tacttttatt tatgattctt
     2761 gaaccatttt ctagtacagc acaatttaaa tctttttcac ctatttttat aataccaaaa
     2821 tgtgttgctc tcaatatact tttttctttt gattccaatt taacaccatc ctttatttta
     2881 ttttaataag taagatatta cttagtatct cttcattaga taaattagta tttataagac
     2941 taataatatc aattttaaaa tatgtatata gataattgag tttttttatc atcttgtacg
     3001 cagtattttt attaacaacg atattttttg ataattttct atagctaata ttttcatcaa
     3061 atataaataa attaatagca agtagccatt ttctgtagtc tagtttagtt ttatgcataa
     3121 tggtattagt tgtaattgaa aacttactat tgcaaatatt gcaatgaaat aaatcattat
     3181 aagtactaat tttatcagaa taacaatagg ggcatattaa tgagtttttt aatttaagat
     3241 tatataaaaa aatcttataa gagacatctg ttggaaagtt tttataaata tcaaagtctt
     3301 ttgtcataat ttaattatat catgttgtag aaattaattt actgtattta agtgggtcaa
     3361 atttaatata tatgtttttg atagtgtaaa aattaaaata taattttagg catgtttgta
     3421 ttgaataatg agcactttca aattttatta gtgtaagagg tttcaaatta taaagtaatg
     3481 aatgtatatt actatatgat ttataattag aataaaattt ttataataga aatttaatat
     3541 atcaaaatat cagttgagga tattgttcaa gtggtatatc ttccctgagt taaaaaataa
     3601 atgatgaaat atttggaaat gctagtcttt atttgtttat gatattatgt aacatagaat
     3661 tttaatatct actttatgat ataatatttt tataaacata agatggttta agttattaat
     3721 taataaaaat atatatatta tataagtgga taggagttga ggtttgtaat ggatgaaaga
     3781 aaactagaat tttctatttt ttgcattgaa gctttagcag aaaagttgaa tatagattca
     3841 gaaggagttt ataaacttct taaacatgat actgatattt tagataacta tataatacct
     3901 tgctatgaag cactacattc tcaaagtaaa agttatatag tggaagattt aatacaagtg
     3961 cttgaagaaa agggcgtgat gtaataattg tatatcatga ttcatactgt gtggcaaaaa
     4021 cgcctaatat tatcttctta agagattctt tagattttgg aaaaggattc tatgtaacca
     4081 gtataaaaac acaggcaatt aactggatag aacgttttaa gaaaaggggt aaaaaagcat
     4141 atttaaatag ctattctcta gatattgata aattaaaaga aaactgcaag gtaaaaatct
     4201 tcaatagtta taatttagaa tggcttgact ttatattaga atgtagaaat atgagtgatg
     4261 tatatatgaa atatgatgtt ataataggtg gaattgctga tgataagatt tacaatacaa
     4321 tagaactgta tcaagataat cttattgaaa aagatgaagc tttaaaaaga ttaaaatact
     4381 ataagccaaa tgaacaaata tgtataacta atcaaaatgt aatagataaa tatttaaagt
     4441 ttagatgtga agaggaggta tagtgcttat ggaagcaaat aaaacattat tacagatact
     4501 atataaaaaa attatattag aattttctaa tcagacaggc aagagtttgg aagaaagtat
     4561 ggattattta tatacttctg aaacatataa acttataagt gagggtgtat cagatatgca
     4621 ctgtaagggt catatatact tggctcaaga attaatgcta gaaaatggat taatgtatca
     4681 taaaggctat ccaagataaa tagttaaaac tagatacgaa ttaaggatac aagataatat
     4741 aaaattaatt tgtatcctta tttgggtttt gtaattaact atatgaaatc atagtatttt
     4801 tatgtatttg atttttacaa tgaaaatatt ttatccaact tttctacagc ttcaaccttc
     4861 ttatcttcca taacttgagt atatatattt aaagttattg atatatcata atgacccatt
     4921 aaaacttgta cagttttagg aggaacatca ttctcaaaaa gtctagttgc ataagttttt
     4981 ctaagaccat gaaactttat aggttctata tctaagctgc ttaatataga ttttaaattc
     5041 cttaaaggtc ttttatcatc tatagggaca cctaattcat tgcaaaatac ataattatca
     5101 tttctatata attctccagc ctttaatata tccacatttt gatttacttt atgctctttc
     5161 aatttattta aaacatcttt tggaataggt acagtcctgt aagaatttga tgtctttggt
     5221 tcttgctcta atactttaag ctctctattt ccatttttat ctataaaata tgttctttga
     5281 agggttcttt taactgttaa attagaattt ttaaaatcaa tatcaaacca tttaagtcct
     5341 aataattctc ctaatctaag tccagtacca agagcaacca aaaataatac ttctaattta
     5401 tgcccctcta tagcttttat aaaattcttt tgttgttcaa gagtcaatat ttcaagccta
     5461 ttttcctttt tctcaactgg tatatttacc atagtacaat aattacgttg tataagacct
     5521 tgtttttcag cttctgataa gcaggttttc agtttagtgt ttatttgcct tatagtaggt
     5581 atacttttat tggaatcaag caatctattg taatattttt gcatgtgaga tactcttaag
     5641 tcctttaact taacattgcc taaatcagta tctttaatat aattacgata gatgctttca
     5701 tatctttcaa atgactttgg ttttaaatct tttattctat attcaaacaa ccaattgtaa
     5761 aaccaatgct ctaaagttat tttatcatct gaaatatcag aacttaaata atattgtttt
     5821 ttataagcat caagtttatc tttaacttcc ttttgagttt taccataaaa ttcttttctt
     5881 attggttttc catcttcatt ataaccaatc attatacgag aacgccaacc atctttatat
     5941 tttccgatag agccttctcc attagctctt ttagttgcca tttttaaaca actccttata
     6001 tgatttatag ttatatccat cgaagaatgt aatacatttc tctaataaga attttataac
     6061 ttcatcagct tctagtgttt catagaagtt tagcattaat gtgttgtatt cttgtttatg
     6121 tttggctggt attgatagaa ctcctatatc attacttata agtattaggt ttgaggtaag
     6181 tcgagcagtt cttttgttac catcatagaa gaattgacaa taggacaacc ataaatttat
     6241 tattatagcc ttttctaagg ggttagttat tttatttatg atacttatgt cacttataaa
     6301 taaattattt aatagagtgg cttctatgca ctcatattga gtagtaccag ctatacctat
     6361 gttaccgttt ctaaaatctc caacaattag agcttcatct tttgcaacta ttttatgtat
     6421 agagcaaatt gtatctttat ttaaagataa gttttctttg ttacttaatt ttattatata
     6481 atcccatgat gattttatat ttagtatttg attttggtca cttaatttat gtccacctac
     6541 tgttatacca tcaagtaatg tttgtacttc tgggaatgtg aaggggttat tttctaggtt
     6601 acccatatca tatacaaatt cacttatcat tcttctaatg tatgttaaag tatacttttt
     6661 atctccatga ggtagattcg tagggaattt atgtgtgtat tttggtatat atgatttatt
     6721 catgatttta gctcctttca tatctaattt ttatttatta actatgaatg tataaaagtt
     6781 aataggtaaa cccatatttt gaaaatgtgc aatactaata ttcttcatcc tcatcttcaa
     6841 taagaccatt agagttttct tgtataaaat tttcaacaag accatttatc acccaattta
     6901 gatttttgga gaagtctttg aagtcagaga agcttaagct aattggtttt attgtatctg
     6961 cattatctgt aatttcaaac ttttttttat agtctgtttt tatatcataa ccataatttt
     7021 ctaatatgtg aactactaaa tccaactctt tatcaaatac ttttaaatta atattattat
     7081 ttgttaaagt atctaaatca ttttcttcaa caatgagttt atttatggca attgataaag
     7141 cagtagatat ttttcttatc atatctagac taggtattct ttgattttgc tcatatttag
     7201 ctatagcatg aactgaacaa ccaatcttgt tggctagctc attccttgac atatttatcg
     7261 atttcctata ttgtttaatc ttttctccga ttgtattatt tatcataatt ccaccttaaa
     7321 tatatcatca aacaatgatt gtatttttac acatataaag gagttatttt ctagattatc
     7381 tatatcatat actaacttac tcataattct tttaatgtat gttagagtat attttttatc
     7441 tccataaaat aaattagtag ggtatttata tgtgtatttt agcatatatg ctttattaat
     7501 aattgtaact ccatttgtag ttttttatca ttgctttaga tagtattgat attattttat
     7561 attttagtta gtagtttttt tatttcttta agtgtatttt caagcttttt atttttttcc
     7621 tttaattcat tattttcttg taataattta aagtaatcat tatttttaat agtcatattt
     7681 tctttttgag atattaactt ttttagtcga atgttactcc cattatctgt tgcctcacta
     7741 aagtaatcat agattgcttc tatattttct tctgatagac caatatctaa taattcatca
     7801 attgattgtt ttataagaac atcattttta ataatttctt ttatatcatc aggatgtaat
     7861 gaaaaatcta attcagtatt agatacacaa tctgaaagta tccaattata aacttgattt
     7921 tcatttagtt ctaatttttc accaagttta taatattggt ctttatcaga ccaagcacct
     7981 agcaataaac ttgaggtaat cctaatatca tcatcttcca taagatttga aatagcattt
     8041 aaaaattcat caaaagaata gtattcttct gggaataaag actgtaaaaa atataaaaca
     8101 tcttcattag tcattacttc attttcaata tcaaaataaa agatagtctt ttcaattttt
     8161 aaagctttag cgagttttga taaggtatct atactaggta ttcttttatc attttcataa
     8221 tttattagag catttcttga gatgccacta atttcagcaa gttgaatctg tgttagttct
     8281 ttttgttttc ttatcttttt taagtttttt ccaatttgca atattatcac ctcgtataaa
     8341 gtatatccca aaagtacaca aatgagaaca gaaaaataat atattcttat tgacagcaca
     8401 catatgtcga cattataatt aaaatgtaaa gtttacgaat gtaaactaga aaaaaggagg
     8461 aaaaagtatg ggaatcaatt taaaaatatt taggattaaa aaaggtttaa agcaacaaga
     8521 actggcagat agagttggta tatcaagata ttatttgtca aacttggaaa caggtaaggc
     8581 taataatcca agcaatgatt tgatgattaa actatctaga gctttagata caacagtaga
     8641 agaattattt tttagtaagg aggagtaaaa tatgaaagag aataataatt tagaagtttt
     8701 aaagacatta caagctactt taaacattat tgtaaagaat gaagaagaag cacaagaaaa
     8761 aaagcaagga tataaaatta ttgaaaatgc gaaaagaaat aaagctgaac ttataacatg
     8821 taaagaagca tctaagcttt atcctatagg agaatcaaaa tttaggcagt tatgtcactc
     8881 taaaaataaa ggtttccctt gtatatggat aaataatcga gtatacataa taaaaaacaa
     8941 attggatgat tggtttattg aaaatgcaaa tggtatcaag ttatagtctt aaagtaggtg
     9001 tttgcaattg agcaataaag ggtggataaa gctttacagg gagttgttgg ataaatcaat
     9061 ttggaaagat attaaaccag aaagaaaagt aatattaata actctattac ttatggctag
     9121 tcataaagaa aatcatgttt ttatagataa taaaaggata aaaataaagt ctggacaatt
     9181 tatttcttct tatagcaaaa tagctgaaag atgtggtaaa ggagttagtt atcaaatggt
     9241 tagaagttgt ttgttggttt ttaaaaatgc tggttttcta acatacgaaa caacaaacaa
     9301 atatatagtc ataactattg aaaactggga gctttaccaa gagaacatag aaaatcaaca
     9361 acctaatcaa cagacgatta acaatcaatc aacaacaatc aagaatataa agaatgaaaa
     9421 taatattata tatagtgaaa ttataagcaa atttaactca acttgtattg aactttcaaa
     9481 ggtcaagaaa ctaacagaca caagaaagag gagaataaat tcaataataa gagaatatga
     9541 taaagaagct gtattagagg ttataaatat agttagtaaa acaagttttc tgaatggaaa
     9601 taataataca ggatggaaag caaattttga ttggataatg aattcaagta attttaccaa
     9661 gatactagag ggcaattatc atagcaagga aattgtcata aataaagaga caggaatata
     9721 taatccagat gattcacaat taaagtggta aggaggttat attataaata attcaaacaa
     9781 cttaattaaa aatacagaag cagagcaatc aatattagga agcattttat tagactcaac
     9841 aatagtagaa gagttagagc aaatgttaaa tgaagacatg ttttatcatg aggaacataa
     9901 atacatattt aatgctataa aagaaataaa taagagaaat gaagtaatag atataataac
     9961 tcttacagaa gaattaaaga gaaaaggtca tttgaataat attggaggta taagctatat
    10021 taccagtttg tcaacaatag tcccaacagt atcaaatttc acgagttatg tcagtatagt
    10081 tattgaaaaa tataaaaata gaaatataat tgataaattc aataagttca atcttggaga
    10141 aatagatgat aatactctta tatatcagtt agaaaatgat ataaataaag ccaatcagaa
    10201 attaataaaa aaggattcaa gtataacagc catatcagag agagcctttg aatatataaa
    10261 tgatgaaatg aatgaaggta ttaagactgg aataaagttc ttagatgata ctataggagg
    10321 tttatatgga ggtgaactaa ctacaatagc agccaaaagt ggaaggggga aaacagcatt
    10381 agctttacaa atacttagaa atgtaatatt tcaaggaaag aaagtacttt ttataagtgg
    10441 agaaatgagt gatatacaga ttctgtttag aaatatagcc tcactaactg gaatatcagt
    10501 tgtaaaaatg aaaaatagaa atctaaatga tgatgaaata gtcaaatata tacaagcttt
    10561 aagcataata aatcaagaaa ataaccttta tataaatgac tctgtaaata cagtatcagg
    10621 gataaagaga gaaataaaga gtactaagcc tagcttagtc ataattgatt atttgcaaat
    10681 tttggattat gaaggtaaag aagtaggaga agctaaattt gctgacttat caaggcaaat
    10741 aaaaaagatt actttagatt ttaatatacc agttattcag cttgctcaat taaatgatga
    10801 attgaaggat gaaagaccta aaggggatag agtaatgaga agctcaaagc agatatatat
    10861 ggattctaat tcagttatat atattcatag acctacagaa aaagaggctc ttaattattg
    10921 caagacaatg ggaattgagg atataagaga gttgagacga agagaaaaag gttttggtgc
    10981 taatttaagt gagattattg ttgccaaaaa tagagatggt gagtcaggat ttagaccata
    11041 ttggtatata ggaaatttgt tttcttttga agaatgtaag aagatacaag agatataaaa
    11101 aagaagcatg atttattaag taatagataa attatgtttc aagaagtatt gaatggtaaa
    11161 tatataaaac ttaattagat gtaatagaga tttaaaatat attgagttta gatttaaatg
    11221 cttattagaa aaaggtagtt gccatataaa tataacaact acctttataa attaggtact
    11281 taatgtacac taacctagca acttaagtat atattaagta cctgatgaaa gtcaaggagg
    11341 tactaaaatg tatgatgaaa ttatagaaat atattttgat tattggataa atgaacatat
    11401 gtatagaagt attttagaaa aagatgagta ttatattgaa tgttggaaag aggttaaaaa
    11461 aattaatgag ttattggaaa taaatttgga agatgataag aaatatctgt tggttgaatt
    11521 aaatgatttg tttaattcat taatagaatt tgaaagagaa cttagttata aattaggatt
    11581 taaaacagga gcaaaattta taatagagtt gttgaagtaa ttaatttgtg tatctcaata
    11641 gaataattaa atagtaaatc gagattatta ataagttgta ctagaatatt ttaagtgaag
    11701 tatatattaa ttatattgtt taaaataaat atatttaata aagaggggat aagtatgccc
    11761 tcttttattt ttatgcaatc aatgacattg taggatttac gtattttgat aaatagcaca
    11821 atatatttta ataataaata tatgatacaa taattttatt aaaatctaaa ataatcaatt
    11881 gaggaggaga atatgataat acctaagtta gagcctaatg atagaaaaag agagtttaat
    11941 aaatactcat cagagcaaat aaacaaagta gtttataatt atttatttaa aaataatatg
    12001 agtaatagaa agatagatga tgagatacta gatttatcat caaaacaaag taaaggttat
    12061 caatcaatgg gtatagtgca tcatttagga ataaataaaa atcataaagg tatttttaca
    12121 ggatacacaa tagataaagc aattgaagtt cttaaggaag atgagataca tttcaagcag
    12181 attattaata tacttttaga aattagaaat ataaatgagt atcctataat agttgactct
    12241 tgggagttat tagatgagaa aatagcttta aaaacaattg ataaatctac attaaaacat
    12301 aatgggacag gagttccaat agaaataaga tatttttttg agataaataa tttaggaaag
    12361 aatgaaaaga aaaatgtgaa ttttaaatat aatgataatt attatagtgc agttatagag
    12421 atggataaat tagagaatcc aagaagtaga ataatttgga aatctaagtt tagagattta
    12481 ctaagaagta tattttctga gtattatgag aaattcttag aaaataaaat agaaggaaaa
    12541 acaaatttac ctaaaataag attcataaaa cttgatagta ataattttaa catagaattc
    12601 atttttactg ctgaaataga agacgatgta ttagaggaga taagtcaaga taatcctttg
    12661 aacattaata ctattaatat agaattagaa actgaaatta gaaaagaagg gaaagttaaa
    12721 tatgtttatg ggaaacagta tgagcgtaac cccagaaata gaatagaagc aataaaatat
    12781 catggcacaa agtgtattgt atgtgggttt gacttcgaaa aaacgtatgg agatagaggt
    12841 aagggataca tagaaattca tcatattaaa ccattaagtt ctgttggaga agaaagcaat
    12901 ataaatccca aaacagattt ggtgccaata tgttcaaatt gtcatagaat gattcacagg
    12961 aagaaagata atgttttgag tattgatgat ttgaagcaaa ttataaaaga tagtaaataa
    13021 tgcttatgtg tggcagagat gtataaagta tatattctaa actagttaac aacaaatact
    13081 aatataaaaa atcaaaaaat ttattaggaa aaggtagttg ctaaagtata acagctactt
    13141 ttataaatta agtatttgag gtatattagt ctaaccgatt aggtatatct taagttattg
    13201 atgaaaatta attaagacta acatgtatga tgaatccata gaaatgtatt tttattgtta
    13261 aataaatgaa catacataga aatattttag aaaattacaa gtactatatt gaaagtttga
    13321 aggagaacct tgatttaagg taagtgtaaa gtttttagaa acttcactaa aataatctaa
    13381 ttaagttata tatttgttac ttttaggaaa ataatggtaa aaattaaagg tttttataaa
    13441 tgaatgtcta attttataaa taaaatggag tattttaact aaatatttga ggagataaaa
    13501 tgatattaaa taaaatcgaa ataattggag atatggctca ctttaaaatt cctatgcata
    13561 gcaagataca aagaacatat gaaattccac caatatcaac agttgtcgga attttaaaaa
    13621 atatatatgg tgaagaaata gatgatttta tattaggata tgttatatat catgatggta
    13681 catttaaaga tttacagact atatataaag aaatcaatcc aaatgtaaaa acattgacag
    13741 atagcgatag atttcaaact gatgtatgtc ccattgaata cctaattaat cctaaactta
    13801 tcatatatac taacatagat aaaaatatag aatttaatga gccgctatgt ttaggtaaga
    13861 ctaattgttt agcgaaggta ttatctttaa gtgaaattga agtaaatcta atagataaaa
    13921 aagctattgg atacaatcaa tatacagata ttaatattgg agatggaatg ataaaaagaa
    13981 taaatacatt gacagagtat aatgaaaaaa aaggttatta tgatatatat tcttcaatgg
    14041 taagagaaaa tgatgagttt gaatatggta aatattatga tgaagatgag caacagaata
    14101 tttttttatg gaaatggaaa aaagggggag taattaaatg catttagtaa gaagacattt
    14161 gatactcaca gtggataatg aagttgtatt agattacaat taccagtatg agctaatgaa
    14221 aaggatatat gaagctatag aaattaatga taaaagaaaa gcactaagtc ttcacaatga
    14281 aggctataag gtggataaga aagtatttaa attatttaat tacacaatta tgtttgaaaa
    14341 tgctaagtat ttaaaagagg gaatacattt aaatccccaa acaaaaatca aattaatttt
    14401 aagtggatat gatgatatat tgaataatat tataaaaggt tttattaaat gtaaagtatt
    14461 taaattgaat aatatagagt ttaaagtttc agatatagaa gaagattcaa agaaaaattt
    14521 taataatata acactttata aagtaaggtc acctattgta gcaagtttat atgatttaaa
    14581 aagtaggaaa caagtatact taaatccaat gcaagaagag ttttataaag cattacatga
    14641 taatttagga aataagtata agcttataca taataaagaa tatactggtg aactatactt
    14701 tgatattgag gatgtattag cagtaaaaaa gaaatatata actaatataa aaggaaaagg
    14761 atttataatt ggatatacag attttgaaat atttgtacaa gctaataaag atatgcaaga
    14821 agtaatttat tattgtggat taggtgaaaa aaatagtata ggaatggggt tactaactta
    14881 tataacttct aggagggctt aatatggata gaagttacaa tgtatttgtt gataatggtc
    14941 tatatgtgtt ggcttattat ctgaacaaag atattaatga tataacttat caagatatag
    15001 agaatagtat agatttaatg agtgataaaa ttgaagaatt tgtaagttgc gaaaaatact
    15061 ctaatttaaa aagtatgtgc tttagcaatt ctacgctaac tcaaccaaaa ggaaaagtta
    15121 ctttaaatga gaagttaaaa ggttttgtta aaaatcaagg agatgaatat tgtagtttat
    15181 gtggtcaata taaggccaaa gtcaaaattg aagataaaga atacaatata gggagaagtt
    15241 atatgcctaa tttggttgca aatacatttt ataatttctc caataatttg caaggtttga
    15301 atgtatgtcc atattgttta gtattgacta tgtattcaat tctgaattgt agagtaagta
    15361 ggtatgcttt tttatacaat tctacatcaa gcgaatttat ggaagattat actttccata
    15421 ttcaaaaaga aaatttaact gatgtagagt taggagcaaa gaaagaaaaa gaaaagtatt
    15481 caattgtaaa attattagaa agtctagtgt gtaaatataa cttatttaat ggtgatatag
    15541 agcaatatat gtttaataac tctggtcaat ctcaggatat tcaggtaaat agaataaaaa
    15601 ataaatatgt gaatttatta ataaaattac aggaaaaagc actactatca aactttgaaa
    15661 agttacattt ggataggtat atcttgaatg gtactttaga aagcaattat ctcagagaag
    15721 tatatagggt aaaaaaggaa gaaaaaatgg atgagaaaga acaaaagaat tttgatgaac
    15781 ttgttatttt tttaaacagg gaggtaaatg tattgaaaaa agtcaaaata gatataataa
    15841 aaaaattaac ggataaatta aaaactagtg acttagatat agacaaaata tataaggagc
    15901 ttaaaatgtt aggaaaattt tatgcatatg aggactggtt agttaactta aatgaaatgt
    15961 attatgaaaa aactggagaa aaactatttg aagcagagga ttatattgag ttggatgata
    16021 taagaagctt tagacagatt aaaaatttaa tattagtaag tttaatttaa taatattatg
    16081 gagggttaaa atggaaaata aaaattcaaa tagaatgtta aatataggct atgcaataga
    16141 agttttatgt gggcgattaa atggaagtag tagtgaagta gaaaagaaaa ataattcata
    16201 taagaattct acttatacta aaaaaatagg gagcagaagt agaatctcaa gtcaatgtca
    16261 aaaatacaat caaaagctat ttatggaaca agtttgtggt tatgaacaaa atgttaaaac
    16321 taaagatggg aaacaagtaa aattggttcc aaacccattt aaatatgcga cagatgatat
    16381 atttgggttt atgatggcgg aaaaattatc tatagaagaa gatgtataca aagaattatc
    16441 agatgaagaa aaagaaatat ataaaaaaaa taaaaagaaa tatgaagtaa atataactaa
    16501 aaaaagaaag tcaagatggc aaatgaactc tttaataaat gtatcaaata gaaaagttga
    16561 atgggagtgg aatgtttgtg gaactacagg agatagtatg ccatatcaag ttgaggtata
    16621 ttcaggggta catacaaata tagcaaatct taatatagga aagataggag aatatgatat
    16681 atctgatata gcaagtgagc atagagatta tacaacttta gaagctaaat caattggagt
    16741 tgaaaagtta gataaagaag aaaagtttaa aagaatagaa gctctcctaa aatcactaga
    16801 atacttaagt gtagaaggta ataagacaaa tcatctaact gatacaaaac ccaaatttgt
    16861 tatattggga gagtattcat ggggaaataa tgtatttcag ggtattatca aagaaaatgg
    16921 cttagatgta gagatgttaa aagaatctat agaacaaaat gaagaattta gaaaaagcga
    16981 tatatggatt ggaataaata aattttatga tgatacattt aatgagatag aagttaataa
    17041 tgaaaaggaa caaataaaga agtatttaga agaagagttt gaagagtatg attttatgaa
    17101 aatatcaaat gtacatcaag cttttgaaag ttacttaaaa tatttaaagg agactttata
    17161 aaggatatat catgaataat ttatatgaat ataaagctaa atcagatggg acaagtatag
    17221 ataagcataa tgaagatatg aattacataa tgaatcaatt agttagcata tataatatat
    17281 ctaatgatat gaaaaatgat ttatatgagg caattgtatg tcatgatgta gggaaagtag
    17341 taaaaagctt tcaagataat attgaaagta gcacaagaag agttagacat gagttattat
    17401 cagcaagtat taagtattta aatagaggtc agaagttagc tatattaacc caccataaag
    17461 atataaagtt tctagagggt caaataacaa attctattta tgaaattgaa ttaaaggaat
    17521 taaaggaact attagatatt gaagtagaag atataagacc atttataaag gaaatatcaa
    17581 gagcaagaaa tgatgatact agaaaacttg aaaatatatt attaaaaggt tacttgcaat
    17641 attgtgacca tatagcttct gcgggaatta aaagtataga taaaggattt aattctatta
    17701 aagattttag tataccaaag ggtgtaagtt ataattcaat acaagaaaaa gttttaaata
    17761 tggataaaaa tgaagatata atgataatgg ctatgacagg tcttggaaaa acagcaacat
    17821 cattgtattg gagtaaccta gttcaaaatg aatataaaag taagagagtg tattatatac
    17881 ttccttatac agcaagcata aattctttat atgaaaattt atataataga aatataagcg
    17941 taagtatgtt acacagtaaa gctccatatt ttttgaataa aattagtgat gaagaagata
    18001 taaaacaact ttaccagata tacaaaaaga gtgtaaaaca aattaatgta tgtactatat
    18061 ttcaattagt aaagtcagta ttttcttgta agagatttga aatgctacta tcacaattaa
    18121 aagatagtat atttatagtt gatgaaatac attgttttga tacaagagta ttgtgttata
    18181 cattggaatt tttaaaatat ttaaaagaaa acttaaatgt aaaaatatgt ataatgagtg
    18241 caagtatacc aacttgctta aaaaagttaa tacaggaaag attgcaaata agtacaatta
    18301 ttgaaccaga tagtaaagat ttaatattta gacatagatt gaatagatta agtgaaggaa
    18361 tacttgatag catacataaa atagaaaatg atttaatgca aggaaaaaga gtattaatat
    18421 gtgttaattc agtagattta tctcaagagt tatatggaag gcttcaaaaa tataatccta
    18481 aattaataca tggtcgattt aatactagag atagagagca ggcagaaaaa gggatagaag
    18541 aaagcttctt attgataggt actcaaagta tagaggtaag tttagatata agttatgatg
    18601 ttctttatac agagttagct cctttagatt cattgttaca aaggtttggt agagtaaata
    18661 gaaggggaga taatgctgag ttgagcaata tttatatata tgattatgat agaaagctta
    18721 atatatatga tgaagatata ataaatagga ctgatatagt aatatcagat ataatacata
    18781 atgataattc tatagttgat gagagtaaag ttccatctta tttagataat gtctatttag
    18841 atatagatat ggataaatat aataaacata aagttgcttt agagattata atgaaaaata
    18901 tgaggttagg ggtatggaat aaatctgcta ataatgatat gttaaataat gatactatta
    18961 gtgttgtgcc tgaatgtctt ttagaagagt acaagttgta tattgataaa aaaatgtatt
    19021 tagaagcgag ttcattattt gtaaatgtat ctgtaaaaag atacaattat aataaaaaga
    19081 tgttttatga atgtggagat atagttgtta caagttatag ttataatgat aaactaggtc
    19141 taatatttga ttgttaagag aattatagta taactaatta aaatatttta ttgagaattt
    19201 gttataattt atatcgacta caaattttat atgttacaat agaataattt tgcagtgagc
    19261 gaaaaatatg tgtaaatggc tgtgagttag tgatggcaag gtttataact gaatttttgt
    19321 ttttagctaa aaacactact ggtgcctcac tgcaaattaa ctattttaat aaccctacaa
    19381 gtgttgtaat tactatagtt ttatttgggg tttaaattac actaagttag ttataaatat
    19441 tccggaaagt acttttgcta tttgattcat tctacccgtt taaattacac taagttagtt
    19501 ataaatttca ctaattttag ttacaactat tacaagtcca gccgtttaaa ttacactaag
    19561 ttagttataa attatgtgta atgtatttta atgttttcta gcaacaatgt ttaaattaca
    19621 ctaaattagt tataaatttt ataaattcta tgaataattt taaagagagg tttcgtttaa
    19681 attacactaa gttagttata aattgtacat ttgacataga agcttcaaaa tccatcccag
    19741 tttaaattac actaagttag ttataaatgt aacagcacga atgatggcac agacctatct
    19801 gcaatgttta aattacacta agttagttat aaatagaagt acacttacaa gtaagataaa
    19861 agacccaggt acagtttaaa ttacactaag ttagttataa attctgtagc tatatgtgga
    19921 agtaatttta gtagagaggt ttaaattaca ctaagttagt tataaattat tgaaggggtg
    19981 agagttgtga ctaggcacta caagtttaga ttacactaaa ttagttgtaa atttacttat
    20041 tcattaagaa acttaactga gagtttgatt tatattattg aattagcaag agttttagaa
    20101 agtagaaaat tataccccaa taatacccca accacaaaaa taaataataa aaaatatcca
    20161 aaaaaattag tataacatca taactaaata gctatatacc tacacttacg aaaaccaaca
    20221 aaacaatcaa ataaccactt gtagactccc ctcgtctcca ccaattttga aaatgaaaat
    20281 aatagaataa aaggaattac ttatgtttgg actaagtaat tccttttatt ttgcttaaaa
    20341 gcacacaatt agcacacaac tagaaggtta tataaataaa aaggctgtcg cactagaaaa
    20401 tagtgctacg ctcttttttg tataaaaaaa taaatcccaa gttcaaaaga acctgggatt
    20461 cttgtataat aactgtatgc taaaacaaaa tactacacaa attgattata ctacttatgg
    20521 tgataattat caatttaagc taccactaaa tattgattac atgattccaa aagatgattc
    20581 tgtaaggtta ttaagttaaa ttattgagga gatgaattta gaaaaattat ataagactta
    20641 ttctcgaatt agatgaaata gagtaatcca tagacaattt ttgaaagtta taatatatac
    20701 agatatgaac tgtatttatt cttcgagaga aatagaaaga acatgcaaaa gagatattaa
    20761 ttttatgtac cttctaggcg aaacctccac tccagaccat tcaacaattg caaaatttcg
    20821 cagtatacat ttttaactaa taggcaaaag cttgattgca cagtttacaa actttcttgg
    20881 agataataat gaaatttcaa aagacacaat atttattgat ggtacaaaaa tagagtcatc
    20941 tgctaataga tatacttttg tttggaaaaa agctgtaaat aaaaatatgg ctaaaatggt
    21001 gaataatata tctgacttca tattgaaatg tgagattgat gttggcatta aaatagttta
    21061 tgaagataaa attaaaatct atcatttaaa gaaactaaaa aaaatatcaa aagaagcaaa
    21121 tgtagcattt attcatggaa aaggaaatag aaaatctact aaaaaccttc atataatggg
    21181 tgaacgaaat agttgttcta aaacagataa cgatgaaact tttatgagaa tgaaagaaga
    21241 tcatatgaaa aatgggcaac tgaaaccagc atataatata caatttggtg tagattaaga
    21301 atatatagtt tggctaaatt caggaaatca aactgctgat actacaacat taataccatt
    21361 tttaaaatct atagaggaat atactaagtt taaatatcct aaagtaatag ctgatggtgg
    21421 atatgaaagt gaataaaact atgcattttt agagcataat aatcagttat ctttcataaa
    21481 acctgcaaat tatgaaatta ttaaaactag aaaatataaa acttatattg gatgtattga
    21541 caatatggat tatgaagaag aaaataatta ttatatttgt acaaatgaga aaaaactatt
    21601 tgctaataaa ataataaata ggaaatcaaa aacaggatat aaaagtgaaa taacatgtta
    21661 catttgtgaa gagtgtagta attgtcaata taaaagcaat tgtattaaga gaagtaatag
    21721 taaggttcct cttaaaaata ggactaaaaa cttacaagta tctaagtcgt ttcatgaaaa
    21781 aagaaaagaa aacttaaaga gaattatgag cctcgagggg gatgaactta gagtgaatag
    21841 aagtatacaa gcagaaggag ctttcgctca agtaaaataa aatatgaaac ttacaagatt
    21901 tttaagccaa ggtaaagaaa atgttttata aggatatatt attgtggcga tagcttataa
    21961 cgtttctaga ctacacaata aaatagtaaa aaataaacgt ggaatatatc tttatcattt
    22021 aaaaaaatat tctaaaatta ttatttataa tcaaatatta tctgaaaaaa tggtattttt
    22081 atatctttat ttgtcatgca taattttact tagaacagaa actttatata aaacaattga
    22141 aaatatacct attaagataa ttagaaaaga gctctcacag aacaaattta atttgctcat
    22201 gcaacagccc catattgata tacatttagg ttgtataatt atacatttca atattacatg
    22261 ttcattagga taatagttag taaatgatat gattgagaga atagatataa taaactaatc
    22321 tagtcctgta ttgcaatgtt tattaaagaa tatattttta aagatccaag tactgcatct
    22381 cttccagaat tgagttggtt aatagttttt tgatgctatg caatttataa taaaaagtat
    22441 aatgatttag ttgatgaatc atcagaattt ataccctcaa agattaatca gattgcaata
    22501 aaaataatga tatattctat agttataata tctattttct ttgcaacccc tcatacaatg
    22561 aatataaata tatcaaactt ggatatgggt ataattctca tttcagtttt atttatacag
    22621 tttttatcta aattaatttt gtatgtatat tttgataaaa gggagatatg taactaagtg
    22681 gtaatattgg tgaaaacaaa aattaaagaa tatagaaaaa aactccttat gacacaaaat
    22741 gaacttgcta aatcagttag ggtaaggtgt gagactattg tacacttgga aaatataatc
    22801 cttctttaaa gttagtgata gatattgcta aagtattcaa tactacagta gaaaatttgt
    22861 ctgaatttat tgaagaagga tagtggatag attataaata gttataagat ggttaatgaa
    22921 atatagttac ttaattatta agtgtgttag aaatatttat atgtaacaca atgtgtagtt
    22981 aagattacct tatctcatct aagactttaa tttttcttcc cataaagagg gtatatcaat
    23041 tttctagtgt ggtagcccat tttttattga tttaaattta taatatatat taatagatga
    23101 tacttaaaaa ttttataaaa tatctaagtt gtcataaata aaacgatata tctaaccgaa
    23161 tatggtaaaa tttgttataa tatagataag ttagagtaaa aataatttta atgggggaga
    23221 aaatgcagtc agtaatatgt aaaatttttg ataataaatt actaattagt aatgatttaa
    23281 gtgagcagga tgatgtatta tatgaggtaa tggataaatt tatagatgat attgaatata
    23341 tttcaaaatt aggagatgag caaattaaaa gtatgcttat ggaggtaaat aaatttgaag
    23401 aagattcaac tgaatttgaa ctatatatga aaaacttatg ctatttaaag caatcatatt
    23461 ttatagatac taatgataaa agtgaaataa attctataga tatagatata ttaaaaaatg
    23521 ttaaaataga ggacttagtt gatataacta atattaaatt agaacgaaat ttaaatataa
    23581 atatagaaca tatatctaaa gctgtaaata aaatatatga agaaaatgaa aagtggaaag
    23641 agtataatat acaaaatact atgtctaaat atataggtat acctgaatca gaagttatat
    23701 ttatatcaaa tttgagtcag tatgtttcat ttttatctga actagataac gcaggcgact
    23761 atgtatctag agggcaaaaa gattgtatgt atgaattatt accatcatta catagaaaat
    23821 ataaaaaaga ctatggtata cattcagatg aatacgagga agcattcaaa caaaaaataa
    23881 tttattatga taaagaaata aaaaatagat cgagtgaaga attaagagct gaagggcaac
    23941 attttggact accaacagaa tatttagatt tcacagaagc tcatttaata tcattattat
    24001 ttgcaataga agactataaa tatacaaaac agcattcaat tgtgtacttt atagactcta
    24061 tttcttataa tactgacact ataaaaagat ctgagaagtt agtaaattat tcagatacat
    24121 ctgcaataga taattgtagt aaattttcat caagatcatt ttttataaag gtagggaatt
    24181 caaatgaaag aatacatttt caaaaaggat gtttcctaaa agtatcatca gaagataaag
    24241 gcgaatttaa ggaaaaatta aaaaagtatt gtaaagtagt agttattaat aaagatgcta
    24301 aaaaagaaat attaaaagaa ttatttaatt taggtattac atttgaaaat atttatccag
    24361 ataaagataa tgtagttaaa tctataaaat tctattatga tgagatagta gggggaaatt
    24421 tataatgaga gaacaattta aaaattctat aaaaaatatt aaatcaaaca gacctcttgt
    24481 atcagtccaa gatattgtga attttattga taaaggagaa aagtacttga aattatcaat
    24541 agatgaaata gatgaaatgg aaattttaca aggaatagtt atgagtcctg attatcaacg
    24601 tacatataaa tcatcaaaca aggaagaaag ttctataatt gaatctcttt taattggaat
    24661 accaatacct gaaatatttc ttgtaaaaat aggtgtaaat gatatgcaaa taagacatgt
    24721 aatggatggg cagcatcgtt tgaatgctat atataggtat gttaagaata aatatccact
    24781 taaaaattta gatatattag gcaatgaccc actatatgat aataaaaggt tttcagaatt
    24841 agagaaaaaa gataaaatta aaatattagg aagtcattta tcaattctag aatttgatgg
    24901 ttttgataat ccagaaatag aaatagaatt atttaaaaga tataacagaa atacaaaacc
    24961 attagagatg caagaaatcg agatggcaac atatttttca gaaactagta agtatataag
    25021 taaatttata aatactctaa taaaagataa atataaggat gaaatagata aatcagaata
    25081 taaatatgct aattacaaat cagatttact tagaatatat aacataacta aatctcgtaa
    25141 tgataaacaa aaaaatcatc aagaaatatg tataatattt agtattttag aaaatgggct
    25201 acaagaaaat atcagagatg gagttacagc ctctaagaaa tttttagagt ataagtcaat
    25261 tcagtataaa aataatgaaa gtgaaaaatt agagttatta aaagataagt ttgatgagtt
    25321 taataggatg atactgaaaa tatctgagaa gatagaatat ccattctcaa caagtttaat
    25381 atctgatgat aagcaaagac aaagtaaata cttgatagga gtatctattg tgttggcttc
    25441 tatttgttat tattatgata tagacttgga aaataattat ttaatacaag atattaaaaa
    25501 aataatatct tgttcaccca tagcagatat agaatataag gcgtcttcaa ctaatatgaa
    25561 aaatatgatt ctatacttat ttataaagaa taaaatacaa gataataaat ttgaatcatt
    25621 aaatttaaaa agagataaaa ttaaagaaat aaatacttta gtaatgaata atgatggtta
    25681 tttaaatgaa cagacaaggc tattagaaat atattaacaa gttataaaat aaatcatggt
    25741 aatgtaaaat aatttgtgct tttaaggtta ataaaaatct tctttatggc aattatttaa
    25801 atataatcaa atgtcgtaaa ggagattttt tatgaacgct ttaagaaaac aattagccaa
    25861 agaaattata tcagatatct aaaaaagagc atgtcaaata aaaccctagg tatttaaatt
    25921 t
//
LOCUS       DAEPOG010000037        25870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEPOG010000037 DAEPOG010000000
VERSION     DAEPOG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.037"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..4126
                     /locus_tag="KR432_001314"
     CDS             <1..4126
                     /locus_tag="KR432_001314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBH0639860.1"
                     /translation="EELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            4330..4791
                     /locus_tag="KR432_001315"
     CDS             4330..4791
                     /locus_tag="KR432_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH0639861.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            4821..5975
                     /gene="nusA"
                     /locus_tag="KR432_001316"
     CDS             4821..5975
                     /gene="nusA"
                     /locus_tag="KR432_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH0639862.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            5982..6263
                     /locus_tag="KR432_001317"
     CDS             5982..6263
                     /locus_tag="KR432_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBH0639863.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            6253..6564
                     /locus_tag="KR432_001318"
     CDS             6253..6564
                     /locus_tag="KR432_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBH0639864.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            6586..8526
                     /gene="infB"
                     /locus_tag="KR432_001319"
     CDS             6586..8526
                     /gene="infB"
                     /locus_tag="KR432_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH0639865.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            8569..8949
                     /gene="rbfA"
                     /locus_tag="KR432_001320"
     CDS             8569..8949
                     /gene="rbfA"
                     /locus_tag="KR432_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH0639866.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            8930..9913
                     /locus_tag="KR432_001321"
     CDS             8930..9913
                     /locus_tag="KR432_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBH0639867.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            9926..10831
                     /gene="truB"
                     /locus_tag="KR432_001322"
     CDS             9926..10831
                     /gene="truB"
                     /locus_tag="KR432_001322"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH0639868.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            10962..11891
                     /locus_tag="KR432_001323"
     CDS             10962..11891
                     /locus_tag="KR432_001323"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH0639869.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            11995..12252
                     /gene="rpsO"
                     /locus_tag="KR432_001324"
     CDS             11995..12252
                     /gene="rpsO"
                     /locus_tag="KR432_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH0639870.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            12492..13337
                     /locus_tag="KR432_001325"
     CDS             12492..13337
                     /locus_tag="KR432_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0639871.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            13462..15573
                     /gene="pnp"
                     /locus_tag="KR432_001326"
     CDS             13462..15573
                     /gene="pnp"
                     /locus_tag="KR432_001326"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH0639872.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            15839..16582
                     /locus_tag="KR432_001327"
     CDS             15839..16582
                     /locus_tag="KR432_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0639873.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            16740..17987
                     /locus_tag="KR432_001328"
     CDS             16740..17987
                     /locus_tag="KR432_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0639874.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            18022..18267
                     /locus_tag="KR432_001329"
     CDS             18022..18267
                     /locus_tag="KR432_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0639875.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            18277..19500
                     /gene="dapG"
                     /locus_tag="KR432_001330"
     CDS             18277..19500
                     /gene="dapG"
                     /locus_tag="KR432_001330"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0639876.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            19613..20347
                     /locus_tag="KR432_001331"
     CDS             19613..20347
                     /locus_tag="KR432_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBH0639877.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            20470..22881
                     /locus_tag="KR432_001332"
     CDS             20470..22881
                     /locus_tag="KR432_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH0639878.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            22884..23942
                     /gene="mnmH"
                     /locus_tag="KR432_001333"
     CDS             22884..23942
                     /gene="mnmH"
                     /locus_tag="KR432_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH0639879.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            23936..25270
                     /gene="rimO"
                     /locus_tag="KR432_001334"
     CDS             23936..25270
                     /gene="rimO"
                     /locus_tag="KR432_001334"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH0639880.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            25257..25799
                     /gene="pgsA"
                     /locus_tag="KR432_001335"
     CDS             25257..25799
                     /gene="pgsA"
                     /locus_tag="KR432_001335"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH0639881.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        10508 a         2693 c         5044 g         7625 t
ORIGIN      
        1 agaagaattg atgtataaac tcgattattt taaagatatg aggatgaaga taagatttac
       61 tggtcttgaa agaaaatcca ataaagatgt tataaaaaaa tattggaata atatattgta
      121 tatattaaag tatctttgtc catctattgc agggtggtat aaacaagtag aatttctttg
      181 tttagaagaa gaattaaaaa taaaacttcc aaaaggtata ttttatgagc gattaatgaa
      241 aaaaaatgta acttatgtac ttaaaaccgt gcttagcgaa gagttgggat tagatttaaa
      301 tataacaata gaaaaagctg ttgatgaaaa ggtaaataaa gaaagactca taagaatcaa
      361 tgatagagaa atggaagaaa aaattagggc tttagaaatt ggaaaagtta ataactgtga
      421 agaaaatgaa gaggaaagct atgtcattaa gtctgatgta gatgaaaatt taatctatgg
      481 agataatgca aatgctatga ttgaaaatat agttgaatta aatgcatctt ctggaacagt
      541 tgcagttgtt ggagatatat ttgatgttga cactaaagaa cttaaaaacg gaaaaatact
      601 tatgatagct tcaattacag attatactag ctcaataagc tgtaaacttt ttttaacaga
      661 tacaaataag gatggagtac ttgaaaatgt taaaaaggga gcatacttaa aaatcaaggg
      721 tgacatagtt tatgatacat atcaaagaga aattagtatg atgattagtg ggataagaaa
      781 agaaactaga atagagagag aagataaatc agaggaaaaa agggtagaac tccatgcaca
      841 tacacaaatg tcttctatgg atgctatttg ttctgttaaa aagcttgtag agcgtgcagc
      901 caaatggggt cattctgcta ttgctataac agaccatggt gttgtacaag gatttccaga
      961 tgctatgaat gcaggtaagt caaataatat aaaaatactt tatggagtag aagggtactt
     1021 ggttgaagat gattctctca ttatagagga tgcaaatgat aaagaacttt cgcagacatt
     1081 tgttgtattt gacatagaga caactggttt ttctaataca aatgataaaa taactgagat
     1141 tggtgctgta aaaatagaaa actttaagat tgtagataga tttagtgagt taataaatcc
     1201 tgaaaaagat atttcctata agatacagga attgacagga ataactaatg aattaattaa
     1261 agacaagcca accattgaag aaattcttcc taagtttatg gaatttgttg gagatagtgt
     1321 attagtagca cataatgcag agtttgatac cggatttata tcgcaaaaat gtagggaaca
     1381 aggcttgtta tataataaca aaaaagtaga tacacttatg ttagcaagag taatgttgcc
     1441 aaacttaaaa agacatagat taaatgtcgt tgcaaaagaa cttggtattc ctcttttaaa
     1501 tcaccataga gcagtggatg atgctgaggc tacagcactt atatttaata agtttttgca
     1561 gatgcttaca gaaaaaggtg caaaaactct aagtgatgta aacaatatac ttggaaagat
     1621 tgattacaca aagcttagca caaatcatat aaccttgata gcaaaaaatt ctataggtat
     1681 aaaaaattta tataaaataa tttcagatgc acatgtaaat cattttttta gagcaccaag
     1741 aatccttaga agtgtattaa atgaatataa agaaggatta ataattggtt cggcttgtga
     1801 agctggggtg gtttttcaag ctgtaaagaa aaacgtgtct gatgaggaaa tgaaaaaaat
     1861 aatagattta tacgattata ttgaagtaat gcctatagat aataatagat ttatgattga
     1921 taaaggagaa gtaaaggatg aagaagagtt aagggagttg aataggaaat taattgatac
     1981 agccaaaaaa tttgacaaaa ttccagtagc tactggggat gttcattttt tagacaaaca
     2041 tgaagctgtc ttaagaaaag ttcttaaata ttctcaaggt tttaaagtag atgaagaaga
     2101 aacttattta cattttagaa caacagatga aatgttggaa gagtttagtt atcttggaga
     2161 agaattggca tatgaagttg tagttgaaaa tagtaatctg attgccgata tggttgaaga
     2221 tataaaacct ataccagatg atacttatcc tccaattata gagggctcag atgtggagtt
     2281 aagagaaatg tgttacaaaa aagccaaacg aatctatgga gacccaatac cagaaatagt
     2341 tcaaaaaaga ctggatagag aattaaattc aataataagc aatgggtatg cagttatgta
     2401 tattatagct caaaaactgg ttgcaaaatc tctttcagat ggatatttag ttggttcaag
     2461 aggctcagtt ggctcatctt ttgcagctac tatgagtgat ataacagaag ttaatcctct
     2521 tccagcacat tatatttgtg aaaacgaaga ctgtaaatat tcattctttt atgaaatagg
     2581 tgaatggggc tcaggtgttg atttaccaga caaggattgt ccaaagtgtg gaagaaaact
     2641 tgctaaaaac ggtcatgata ttccatttga ggttttctta ggttttgaag gtgataagga
     2701 accagatata gacttaaatt tctcaggaaa ttatcagcca acaattcata aatacacaga
     2761 agaactattt ggagaggggt atgtatatag ggctgggact ataggtactg tagcagaaaa
     2821 aacagctttt ggatacgcaa gaaaatatgt ggaagaaaat aatataagtg taccaaatgc
     2881 agaagtactt aggctttcaa atggatgtac tggagtaaaa agaacctcag gacaacatcc
     2941 tggtggagtt atggttatac cagactataa agatgtatac gattttacac ctatacagta
     3001 tccagcaaat gatacttctt gtggtgttat aactactcac tttgattatc attcaataag
     3061 tggtagaata cttaaacttg atatacttgg acacgacggt cctaccatta taaggatgct
     3121 tgaagatatg actggaataa atataacaga aattccttta gatgataaag agactatgtc
     3181 tctatttaca tctacagaag cattaggtgt tacaccagaa gaaataaact gtcctatagg
     3241 ttgccttgct gtaccagagt ttggaactaa gtttgtacga cagatgttac ttgacaccaa
     3301 acccactaca tttgcagaac ttgtgcgtat atctggtctc tctcatggga cagatgtttg
     3361 ggtaaataat gcacaagact tagttagaga agatatagtt ggattaaaag atgttatatc
     3421 tacacgagat gacataatga actatttaat atttaaagga ttacctccca aaatgagttt
     3481 tacaataatg gagagcgtaa gaaaaggtaa aggtctaaaa ccagaacaca ttgaagaaat
     3541 gaaaaaaaat aatgttccag aatggtatat aggctcatgt aaaaagatta agtacatgtt
     3601 cccaaaagca catgctgttg cttatgtaat gacatcattt agaattgcat attgtaaagt
     3661 tcactatcca gaagcatttt atgcgactta ttttacgaca aaagttgagg attttgatgc
     3721 agatttaatt gttaaaggat tagatgctat aaaatcaaaa ataaatgaaa ttgagtcact
     3781 gggaaatgat gcaaccacaa aagaaaaagg tatgttgacc gttttagaag ttgctcttga
     3841 aatgtatgct agaggaataa aattattacc agttgatata tataaatcag atgcaactga
     3901 gtttatagtt gtaggagaaa aaacattact tccaccaatg gcagcaatac aagggcttgg
     3961 aggaaatgcg gctataaaca tccaaaatga aagaaaaaat ggtgaattta tatcaaaaga
     4021 agatttgaga aagagaacaa aaatatcaaa aacggtaata gaaactctta ctaatcatgg
     4081 ttcacttgag aatatgagtg agagaaatca attgtcattg ttttaagtta gcccaaaaag
     4141 ttgcaaataa aagaatttta tgatatcata gtatagaaaa tagtatactt tgcagaaact
     4201 aagagtgaga cagctgttct cactctttta ttttaaatat attattaaaa agttggttta
     4261 tttttattaa atatatttta tttaagatta tataaactaa tcatttacaa ttgaatagga
     4321 ggaactttaa tgaaaaaaaa tttagaagct acaattgagg agattgtaac taaaataacg
     4381 gatgaacatg gatttgaaat ggttgatgtt gaatatgtaa aagaagcagg agaatactac
     4441 ttaagagtat acatagataa agaagaagga atatctttaa atgaatgtga attagttagt
     4501 agagaattaa gtccaatact agatgaaaaa gaccctataa aagaaaatta ctttttagag
     4561 gtatcttctc caggacttga tagagcatta aaaaaagaca gagattttgt tagatatcaa
     4621 ggtagagatg tagatttaaa attatataaa ccattaaatg gatgtaagca atttgaaggt
     4681 gaacttgtag gattaacaga agataataat ataaaaatca ttgcaaatgg taaggaaatg
     4741 gaatttaata gaaaagatgt tgctattgtc agattagcaa taaagtttta gtggtatatt
     4801 aaatagggag gatataaaaa atgaatcatg aatttatgga agcattagat gaattagtag
     4861 ttgacagagg tatagataaa gaaatactta tagatactat agaacaagct cttttaactg
     4921 catacaagaa aaactttggt tcagcacaaa atgttagagt tgaatttgat agagacaagg
     4981 gagatataaa agtattttct caaagagttg ttgttgatga atcagattta tatgatactt
     5041 tcttagaaat agagttagcg gatgctagag agataaaccc taattatgaa ttaggtgata
     5101 ttatagaaca tgaagttaca cctaaaaact ttggaagaat tgcagctcaa acagcaaagc
     5161 aagtagttgt tcaaagaata agggaagctg agagagaaat agtgtataat gaatttatag
     5221 aaaaagaaaa tgaaatagtt acaggagagg ttgctagagc aaataaaaat gttgtttatg
     5281 taaatcttgg aagaatagaa ggtataatga ctcaaagtga acaaatacca tttgagaatt
     5341 ataaagcagg tcaaaaaata aaattctata tacttgaagt taaaaagact aacaaaggac
     5401 ctcaaatagt tgtatcaaga agtcatccgg gtctagtaaa gagattgttt gaagaagaag
     5461 ttcctgaaat atatgaagga atagttcaaa taaaatctgt agcaagagaa gcagggtcaa
     5521 gaactaaaat ggcagtaaaa tctatagatg aaaaaataga cccaattgga gcttgtgttg
     5581 gaccaaaagg acttagagta aaaaatatag tggatgaact tggcgatgag aaaatagata
     5641 taataaaata tagtgaagac ccagcagagt ttatatcagc agcacttagc ccatcaaaag
     5701 ttgttagagt agatatagat gaagatgaaa agtctgcctt agtaatcgtt ccagattatc
     5761 agttatcgct tgctataggt aaagaaggtc aaaatgcaag actagcagca aaattgacta
     5821 actggaaaat agatattaag agcgaatctc aagcagaaca agaaatgtta aatcttgaaa
     5881 attccaaaga aatagaagct gaggatgttg aagctgaatt tatcgaccaa gaagagttag
     5941 gtacttcaga ggataatttt agtgaagaag agtaaggagg gatgattttg aaaaaagtaa
     6001 aaaaagtgcc tcaaagaaaa tgcatagcat gtcaagatag agactctaaa aaagagctta
     6061 taagaatagt taaaaataaa gaaggaaaaa tatttttaga cccaacagga agagccaatg
     6121 gaagaggagc atatgtatgt aaaagctctg aatgtctaaa aaaagctata aagagtaaag
     6181 cattaaataa agcttttaaa atagatgttc cagatgaagt ctatgataat ctacttatgg
     6241 agttagaaga atatgaaaaa taataaagaa aaagtacttt cgttcttagg gctcgcaact
     6301 cgtgcaggga aaatagtgtc tggtgatgat tcaactttgc ttgaccttaa aaaaggaaaa
     6361 gtaaatttga ttttaatcgc agaagatgca tcaaataata ctaaaaaact ttttaaagat
     6421 aaatcaactt ttagaaatat tccatatcta tttttttcaa caaaagagga aataggattt
     6481 gcaataggaa aatctccaag agcagttgta ggaataaaag atgaaaattt ttctaaaaag
     6541 ataattgaac taatagaaat ttaaaataaa atagggggtg atcttgtgtc aaaaacaaga
     6601 gtataccaaa tagcagaaga attaaatata tcaaatgaag agcttataaa taagttagct
     6661 gaattagata taaatgtaac tgacaaagat tcagtattag aaggtgaaga gttagaatta
     6721 gcacttgaaa tgttaggtga agatttatct caagaaaatg gaaatgttat agaaatagat
     6781 ggaaagttga cagtacaagt attagcaacg aaattagata aatctccatc agaaataatt
     6841 atgaagctta tgaaaatggg aactatggca acaataaatc aagagataag ttttgagatt
     6901 gctgctttgg ctgccaaaga ctatggattt gaattaacag tagctgaaag tgatgataca
     6961 gaagctttag aaatagaagc tttaatggaa attgaagaag ataaagaaga ggatttaaaa
     7021 cctagaccac cagttgttac agttatggga catgttgacc atggtaagac atctttacta
     7081 gatgcaataa gaaaaactga tgtgatttca ggtgaagctg gaggaataac tcagcatata
     7141 ggtgcttcag aagttaaaat caatggacac aaaatagtat ttttagatac tccaggacat
     7201 gaggctttca catcaatgag agcaagagga gcacaagtta cagatatagc aatattagta
     7261 gttgcagcag atgatggtat aatgcctcaa acagttgaag ctataaacca tgctaaagca
     7321 gcaggtgtac ctttaatagt tgctattaat aaaatagata aaccaggtgc aaacccagat
     7381 aaggttaaac aggaattagc agaccaagga ttattagttg aagactgggg tggagaagta
     7441 atagcagttc cagtatcagc taagaaaaaa gaaggaatag atacactttt agaaatggta
     7501 ttattggttg ctgaaatgga agagttaaga gctaatccaa ataaaagagc agtaggaact
     7561 gttatagaag ctgagcttga taaaggaaga gggccagttg caacagtact tgttcaaggt
     7621 ggtactttaa cagtaggtga cccaatcgtt gcaggtgttg catgtggtaa agttagagca
     7681 atgataaatg caaaaggtaa gagagtaaaa acagcaggac catctacagc agttgagata
     7741 ttaggtctat cagaagttcc acaaggagga gaccaatttg tcgaagttcc aactgataag
     7801 attgctagaa gtgtagcagc aagaagacaa caaatagtta gagatgaaat gttaaaatca
     7861 actcaaagat tatcactaga tgctctattt agtcaaatga gtgaaggttc tataaaagac
     7921 cttaatatag ttataaaagc agatgtacaa ggttcagtac aggcagttaa acaatcttta
     7981 gaaaaattat caaatgaaga agttcaagtt aaagtaatac atggtggtgt tggagctgta
     8041 acagaatcag acatattact tgcagcagct tctaacgcaa taataatagg attcaatgta
     8101 agaccagttc caggtgcaga atctctagga gaaaaagaaa atgttgatat aagaacatat
     8161 actataatat acaaggcaat agaagatata caagcggcta tgactggtat gttagaccct
     8221 gaatatgttg atgaagaaac aggaaaagct gaaataagag aaatatataa aatatctggt
     8281 gttggaacag tagctggatg ttatgtgact aatggtaaga tatttagaaa ttgtaaagta
     8341 agattagtta gagatagcat aataatacat gaaggtgagc ttgcagcact taagagattt
     8401 aaagatgatg taaaagaagt taattctgga tacgaatgtg gtatgagttt tgtaaattat
     8461 aacgacataa aagaaggcga tatagtagaa gcttatataa ctaaagaagt agaaagaaaa
     8521 ttataaattc gtatcaaaga aaatcagtta gtaaagaagg tcgatggtat ggcatcatat
     8581 aatagaacaa gaaggatagc agaagaaata agaaaagtcg taagtactat gcttataaat
     8641 ggagtaaaag accctagaat aacatctatg gtgagtgtaa ctgatgtaga agttacaaat
     8701 gacctaagat atgcttatgt atatgtaagt attctaggtg gagatgaaga atccacacta
     8761 acaggtctta aaagtgctgg aggatatata agaagagaag taggaaaaaa tataaaactt
     8821 agatatattc ctgagatagt atttaaactt gatgattcaa tagaaaaagg aatgtatatg
     8881 gataacctta taaaaagagt taatgaaaaa aatgctcaac aaaatgagga tgaaaattat
     8941 gatgaataat atggatagta ttataaacta tatttatgag ggtgactatt ttatagttac
     9001 ctctcatatc agtccagatg gagacaatgt tggttctaca ctttcaatgt attatacatt
     9061 gaagaattta ggaaaaaatg tatattatgt acttgatgat gaagctccat taaatttaag
     9121 gtttttggtt gaaggtgtaa aaatctataa gtctaatgaa tttaatatga agaactattc
     9181 tttaattgca cttgattgtg gggataagat gagaatatgt gtgagtgatg aaatcaaaaa
     9241 taatgcatca aaattaatct gtattgacca tcatgctagt aatgattcat atggagattt
     9301 aaattatgta gatattgatg catcttctac atgtgaacta gtttataatt tattggttag
     9361 atttcagcaa acaaaagata ttaatattat aaatgaagat atagcaacat gtttatatac
     9421 aggtcttgta actgatactg gaaacttttc gtattcaaat gtacatgcaa gtagttttga
     9481 aatggcaaaa aatttacttg ttttaggtgc acaaaaaaat actataatac aaaatatata
     9541 tcaaagcaat tcttctgatt attataaatt attaggagaa gctctaaaag gtttggagat
     9601 atttgattca aaggtatcta gtatagtttt aactcaagac atgatgaata gaaataatat
     9661 tagctttaat gatgttgatg gaataacaac atatacaaga gatataaaag gtatagaagt
     9721 tggaatttta tttaaagaga aaaaacaaaa tgaaattaaa gtgagtttta ggtcaaaaaa
     9781 ttatgttgat gtaagtgaaa tagccaagtt atttggcggt ggtggtcatg taagggctgc
     9841 tggatgtact attagagata gtattgataa tgcaaagaaa atggtactag aggcagtgtt
     9901 aaaatcaatt taaggtgaca gtaaaatgaa taagattata agtatattga aacccacagg
     9961 tatgacatct catgatgttg taagtagagt gagaaagatt ttaaatataa aaaaagttgg
    10021 tcatacaggt actcttgacc cagatgcttc aggtgtttta ccaatctgta tagggaaagc
    10081 tacaaaagta tgtgaagtta ttttaaacaa agataaatcg tatatatgtg agcttacttt
    10141 aggtataagt acagatactt atgatgcttc aggtgaaatt ttaaaaaaag ttgatgattt
    10201 taaatttagt aatgaagaca tagaaagagc ttttgataca caaagaggag agataaatca
    10261 acttcctcca atttattcag ctttaaaggt aaatggaaag agaatgtgtg atttagtaag
    10321 aagtggtaga caatctgaaa ttactttaaa aacaagaaga gtaaatataa aagacataaa
    10381 aatactaagt ataaaaggta ataaagtaat gttttatgtt gagtgttcta aagggactta
    10441 tgtgagaagt atttgccatg atataggaga atatctgggt tgtggagctc atatgtcgtt
    10501 tttgaataga acatcttctg gtaaatttga cttagataat tcaataacat tagaagaatt
    10561 agaattattt tatgaaaata aaactttaga caaatactta tatgatatag attatgtcct
    10621 tgattcgttt aattatgttg tattaaaccc taatgctata aaatattata gtaatggtgg
    10681 ctcaatagat gataaaaggt ttttaaaaaa taatttcgat aaagatgatg aagttgtaag
    10741 agtatacagt acagataatt tcttaggatt aggaaagtta tcaaaacaca ataatacaat
    10801 aagtgttaaa agtgataaga tgttcatata accaatgtat ttaaaaaaac aacaaaagag
    10861 atagtactat aataacttct atagtctatc tcttttactt atgctataat agtttgtgaa
    10921 taagttaaga gtaaattata ttttagcggg gaacatttat tatggtgatt ataaaatcta
    10981 taaaagaaat agcaaacata aaagaaagtg tcattaccat aggaaatttt gatggtgtac
    11041 acaaaggaca tcaagttctt ataggaaaaa cagttgaata tgcaaaaaaa gaaaatatac
    11101 agagtattgt atttacattt gcaaatcatc ctgtaaatta ctttagacca aactcaacaa
    11161 aaaatattat aagcaatgat gataaaataa aattattaga agatttggga atagatatag
    11221 ttgtaatcat accatttgat gagtatatga ctaaaatacc tgcaaaggat ttcgtagatg
    11281 agatactagt aaaaaaatta aaagctaaaa agatagtaat tggacatgac tttacatttg
    11341 caagggcaaa agaaggcaat gttaatctat taaaaagctt agaacatgaa tttggttttg
    11401 aggtagaagt aataaaaccc atcaaaatca atgatgtaag agtgagtagt acttatatta
    11461 gaagtttagt atcccagggt gatatggcta atgtcaagga atatttgggt agaaattaca
    11521 agttagaagg ttgtgttata cattctaaac accttggaag aactatagga ttcccaacgg
    11581 caaatattga ccttaagaat aatatgctcg ttccaaagag aggtatttat gcttctattg
    11641 tacatattgg agatgagact tattttggtg ctacaaatgt aggctataac ccaacagtta
    11701 atggaaaaac tttgtctatt gaaacaaata ttttagagtt tgatagagat atttatgggg
    11761 aaaatataat tgttgaattt ttggaaagaa taagagatga aagaaagttt aattcaatag
    11821 atgacttaaa aaatcaattg tataatgata caaattatgt gtatgaaaac tatgtttgca
    11881 aaaacaagta atgtatgata aaatgattaa ggaaaataac cattactcgg catttgaata
    11941 ctccaatcaa tgcttagtaa taggcgtttc taaaaaaata cggaggtaat ctaaatgata
    12001 aacaaatcag aaataataaa agaatatgga agagctgaag gggatactgg ttctccagaa
    12061 gtacaaatag ctttattaac agctagaata aacgagttaa atggacattt aaaaactcat
    12121 aagaaagacc accattcaag aagaggacta ttaaaaatgg ttggtagaag aagaaactta
    12181 ttagcttact taaaggaaaa agatcttgaa ggatatagag cattaatagc taaattagga
    12241 ttaagaaagt aatttcattc tttataaaaa agcaggtata gtgtatacct gcttttttat
    12301 agttaattca ttataaagta aaatagttaa taataaaagt agtttatatg ttataaaaaa
    12361 ttataaaatg aataatattg ggtaagaata ttaaaatagt atacaaatac ataatcaatt
    12421 taaaaggaaa gacaggataa atatagaaat tttataacag gataattagt tggaataagg
    12481 aggatattat tttgaaaata aagttagtat gcgatagttt gtgcgatatt ccagatgaaa
    12541 tatcagaaaa agattatata gagatagttc cacttacagt tatttttaat gatagggaat
    12601 atatagaagg agtagatata aagaaagaag agttttataa aaaagtaaaa gaaatcaagc
    12661 aaatacctaa aacttcacaa gctacatata tggagtttaa agaagtgttt gataagttca
    12721 taacagaagg ttatcatata atctgtatga caggggctgc aaacgcatca ggaacttttc
    12781 aaagtgctat gatagctaaa aatgatgtaa atgaaaatga aaaaatacat atatttgata
    12841 ctagaaattt atctctaggt agtggacaat atgttataaa ggcatgtgaa cttcttgaag
    12901 aaggactggg atttgaagaa attatagatg aattagaaaa tactcgcagt agtgtaagat
    12961 tactttttgc accatataca cttgattttt taaagcagag tggaagagtt ccagttgcaa
    13021 ctgctttgat aggaaatatg ttaaatataa agcctatatt tttctttgat aatggggaag
    13081 ctaagttggt aaataaggtt agaggaataa agaatatagc aagtaaactg gtcgatataa
    13141 ttttagaaat gaatgaaggg tctttagaag gaaaaatcgt cacaataggt tgtggagata
    13201 atttacacga ttgtgaaata ttaaaagatg aagtaaataa aagaataaaa gcaagaagag
    13261 tattatttac tcgaggagga gtttctattt gctctcatac agggccagat atattggcta
    13321 taagttgttc taattagtag taatataagg tttttgcata tatatgtgaa gataatttta
    13381 tattgttaat ttatagtatt tttgataata tagtaaatag agtgatatac gtgtttttta
    13441 ataagaggag gacaagcatt aatgtttgaa cataaaatat ttaaaatgga ttttgcagga
    13501 agagaacttt ctgtagaaat aggaaagatt tgtgaaatgg ctagtggtag ttgtatagtt
    13561 cgatacagtg acagtatggt aatggtaaat acaactaaat cagccaaacc tagagacgga
    13621 atagactttt ttcctttaag tgtggattat gaagaaaaat tatactctgt aggtaagata
    13681 cctggaggtt ttttaaagag agaagggaag ccttcagaaa aagctatact tacatcaaga
    13741 ttgatagata gaccaataag acctttattc ccaaaaggat ttagaaatga tgttcaggta
    13801 gttgcaacag ttctatcagt agaccaagat tgtacaccag atatagtagc aatgataggt
    13861 tcttcaatag ccctttctat atcagacata ccatttaatg gaccaacagg ttcagtatgt
    13921 gttggccttg tagatggtgc atttgttgta aatccaaacg cagagcaaag agaaaaaagt
    13981 tcaatgcatc tagttgtttc aggtacaaag gaagcaataa tgatggttga agccggagct
    14041 gatgaagttc cagatgaggt aatgcttgat gctattttat ttgctcacca agaaataaaa
    14101 aaaatagttg agtttataga aggtatagtt gcagaagttg gaaaagaaaa aatgcctgtt
    14161 gaattatacc atgcaggaga agaaataact caattggttc gcgaatttgc aactgataag
    14221 atgaaaaaag cagttcagac ttttgaaaag ttagagagaa tggaaaatat ggatagagtt
    14281 aaagaagaaa ctttagctca ctttgaagaa actttagaag attttgaaga ttttgttgga
    14341 gatatagaag aagtattaca agatataata aaagaagaag taagaaaact tatagtacat
    14401 gaaaatgtaa gaccagataa tagaaaactt gaagaaataa gacctatatg gtgtgaaact
    14461 ggcatgatac ctagagctca tggttcagcc atatttacta gaggacaaac tcaagtactt
    14521 aatgttgcta ctttaggtgc tttaggagat gttcaaaaat tagatgggtt agatgaagaa
    14581 gaaaacaaga gatatatgca tcattataat ttccccgcat atagtgttgg agaagctaga
    14641 ccatcaagag gaccaggaag aagagaaata ggacatggtg ctttagcaga aagagcttta
    14701 ttaccagtaa taccatctca agaagaattt ccttatgcta ttagattagt ttctgaagta
    14761 ttaagttcaa atggttctac atctcaagca agtgtatgtg gctcaacatt atctttactt
    14821 gatgcaggtg tacctataaa agatatggtt gcaggtatag caatgggact tataaagcat
    14881 gatggtaagg ttgcagtgct ttcagatata caaggtatgg aagaccattt aggcgatatg
    14941 gactttaaag tagctggaac agagtatggt ataacagcaa tacaaatgga tataaaaatt
    15001 gatggtatag ataaagaaat tctacaacgt gctttaaaac aagctaaaga gggtagaatc
    15061 catatactag gagagatgag aaaaactata tctcagccaa aaccagaact ttcaccatat
    15121 gcacctaaga ttgttaaaat gcaaataaat cctgacaaga taaaagatgt aatagggcct
    15181 ggtggaaaga taataactaa gataatagac gaaactggag ttaagataga tattgaacaa
    15241 actggggaag tatttatatc aggtattgag attgatatga ttaagaaagc tcaagaactt
    15301 ataaataata tagtagttga accagaagta ggaaaaactt ataaaggtaa ggtaagtaga
    15361 ataatgaact ttggagcatt tgttgaaata cttccaggaa aagaaggatt acttcatata
    15421 tctcatatag ctcatgaaag agttgccaaa gttgaagatg tattgaatat aggggatgaa
    15481 gttgaagtta aagttacaga gattgatgaa aaaggaagag ttaatttatc aagaaaagtt
    15541 cttttaccaa aaccagaaca taaaaataaa taacttatta tataattaaa agcaaagtct
    15601 taggattttg cttttttaaa tacaaaaaag tagataattt taaaatgata gttatggatg
    15661 gcttatagtt ctttttctgt aaggctttac tcttataaag aagctattta aaagctgatt
    15721 aatagaaagc tttattgata tatatagaat cacttaagtt atatttagtg cataaacaga
    15781 gcttattttt aatagtatta aataagaaac aatttgtaca taattagggg gagttttaat
    15841 gataatgatg gtacttaata agagtaagct caaaaaaatc ataatactaa ttttagtagt
    15901 gttattagta ttatcaggag gggtaatggt attaaagtta tttaacaaca agccagtatt
    15961 taatttattt aatggtgtag atttaccata tgaaagaggt acaaaggata aaagtggata
    16021 tgttgcatta acctgtaata tagaccttgg atgggaaaca gagtacgtag aatccatttt
    16081 agaaacactt aaaaaagaaa atgtaaagat tacatttaat gtaactggaa aatgggcaga
    16141 aaagaataag gatgaattat taaagataaa aaaacaagga catgaaatag gtaatcatgg
    16201 atataaacat ttagattatt ctacattatc atatgaagac aattatgagc aaatagagac
    16261 ctctaaaaag ataattgaag aaattatagg tgaaaaaact aaattttttc aagcaccagc
    16321 aggttccttt ggtccagaaa cagtaaaggc tgctaaagca ttagggtata cttctatcaa
    16381 atgggatgcc gatactatag attggaaata caaagaccaa ccagaggtaa tcattgatag
    16441 aatgaagaaa aaagacatta aagatagtag tataatctta atgcatccaa ccaacgcaac
    16501 gacaaaatgt atagatgata ttatagctat tgtaagagaa aaaggactaa aaccagggaa
    16561 acttagtgat gtatttaagt agacaaagta taaatatttg gttaagattg tataagttgt
    16621 agttgaatta attaaaagac tgacttataa tgtaatacat ataaaaaata aatataatta
    16681 aaacatagtg ttttcaataa tccaatatga aacaaagaat aaattgttag gagaatgtaa
    16741 tgtataagac aaaaatactg gaaaatgggc ttacaataat tggtgaagaa ataccatatc
    16801 taaagtcaat aacattaggt atttggataa atgcaggctc taggatagaa gaagcacagg
    16861 tgagtggaac ttctcatttt atagagcata tgatgtttaa aggaaccaaa aacagaacat
    16921 caaaagagat tgctagttca atagataatt tgggtggtca aataaatgca tttacaagta
    16981 aagaatgtac atgttattat gtaaaactaa tagatgagca tatagatacg ggaattgatg
    17041 tattgagtga tatgatacta aattctaaat ttgataaaaa tgatatagat aaggaaagac
    17101 ttataatatt ggaagaactt aagatgtatg aagactctcc agatgactta tcttatgatt
    17161 tattagtgga aaatatatat gctaatgatg gattaggaat gaatataatt gggacaaaag
    17221 aatctctata caatataact agagaatcca tgttagaata cttaaataag tattatattc
    17281 caaataacgc tgttatttct atagctggaa actttaactt tgatgatatg gtggagaaaa
    17341 tcaaatcaaa atttggacac tgggaaaaga aaaatttgag tatagacata agtgaggcta
    17401 agtttaatcc ttgctttata tcaaaaaata aagatactga acaggtgaac ttagctatct
    17461 gcctaaaagg tattcctttt gaaaatgatg aagaagtcta ctcaatggca gtggtaaata
    17521 atattttcgg tggaagtata agctcaaggc tttttcagaa aataagggaa gaaaaaggtt
    17581 tagtatattc aatatactca tcacagacat tatataggaa atgtggtgaa ttaggcatat
    17641 ttgcaagtat gagtacagaa aatttacaag atgtatataa tttaataaag aaagaaattg
    17701 aaaatataag agaaaactat cttacagaaa aagaaatatc tgaaagcaag gaacagttaa
    17761 aaggaaatta tatattggat ttagaaagta ctagcagtag aatgatgtct actggtaaat
    17821 ctatgttact tagcaaaaaa gtaaaaacta cagatgaaat acttgaatgt ataaataatg
    17881 ttaatattaa ttcaataaag aaggttgttg ataaggtatt caatatcgaa aatattggta
    17941 catgtatagt tggaagagat gtagaaaaga ttctacactt ggattaatat tgaaaaaagt
    18001 aaatctctgg aggtataaaa tatgaattta tctgaaatag caggtaagga aatagttaac
    18061 ttggtcacag gagaaagatt gggggtcatt ggagaatgtg accttatact agatgaaact
    18121 acaggcggta tattagcgct tctaattcca aaggaaaaag gattttttgc cagaagaaaa
    18181 gataagtcat ttttagaagt accatggaga aatgtaaaaa aaataggtaa tgacatgata
    18241 attatagaat atgagggata tatttaggag gaaaaaatga gtattttagt gcaaaagttt
    18301 ggtgggacat cagtagaatc atatgaaaaa atgaatgaag tatgtaagat agtaaaagca
    18361 tacaaaaaaa atgatgaaga attacaatta gtattagtag tttcagcaat gggaagaaaa
    18421 ggagctccat atgctacaga tacacttata aatttgtgta gtgcagtaaa tgatgagcca
    18481 agtaaaagag aacttgattt aataatgtct tgtggagaaa taatatcagg aacaattctt
    18541 gctaatttat tgaatgcaca ggggatagat tctgttttct ttactggtca gcaagcaggt
    18601 ataattacaa gtgatgaata ttctaatgca aagataaagt atataaatcc taaaaaggta
    18661 aaaagagctt tagatgatgg taaggttgta gttatagctg gattccaggg tgtaacagat
    18721 gatggagaaa taactactct tggaagagga ggaagcgata catcagcagt tgctattggt
    18781 aaagctttag aatgtgaaac agttgaaatt tacactgatg tggatggtat aatgactgca
    18841 gaccctagag tagaaccaaa cgctaaagta ttgagtttta tagattatga ggaagtattc
    18901 caaatggctg acaaaggtgc aaaagtaata catccaagag cagtacagtt ggctaaaagt
    18961 ggaaatataa ctttagccat aaagaataca atgaatccaa cttttgaagg tacaaaaata
    19021 ggttctttat gtagacactt agaagagaat atagaatatg aacaagaaag agattttaag
    19081 gttgctgttg caaataaaga tagtgtagca caagttaaga ttaaatcagc agaagaagtg
    19141 tttacagaag tattaaatga gatagaaaag aaacttataa ctatagatat gataaatttc
    19201 tttatatcag aaaaagcatt tgttgtagaa gatgctgata taaaatcatt aaaggaaata
    19261 ctagataaat ttgaattaga ttatgaagta aatagagact gtgctaaggt aacattgata
    19321 tgttcaagaa ttgctgatga aatgtctggt atcatgtcaa aggtagttag aggattatca
    19381 aaagcagggg tatctctttt acaaacatca gactcaaata tgactatatc ttgtttagtt
    19441 agtgaagaag atatgcatac agcagtacat gctatacacc aacaatttta tttaaaataa
    19501 actgtataat acaattattt tttaatcaaa tttaagaatt tcatatatat cttaatatat
    19561 ttgatttatt tggtaaaact aaattaaata taattttgat aggagttttt ttatgaaaga
    19621 ttatagagaa gataatgaaa gatttgataa agataattgt agtgaagaat ctagttgcga
    19681 agagagagaa tgtataaagc aatttggtac caatgaaatg ccacctcaac ctcctaaaga
    19741 tattcaatgt ataactataa ttggagaaat tgagggacat tttataggaa atcctcaaaa
    19801 aaaagctaca aagtacgagc atataattcc aatgctatac tcaattgagg aaagcaatga
    19861 tgttaaagga gtgttagttg ttttaaacac tgttggagga gatattgaag caggtttagc
    19921 tattgcagaa cttttaaaca gtacatctaa aaaagtagtt actcttgttc taggaggaag
    19981 tcatagtata ggtgttcctc ttgctacagc aggagattac tcatttatag caccaacagc
    20041 aaccatgatt atacatccag tgcgaactac tggtcttgtg attggtataa atgagacttt
    20101 tgaatatttt aaaaagatgc aagatagaat aattcaattt ataataagaa catcaaatat
    20161 caaaaaagat gtacttgaaa aacttatgca tgaaaaagat gaacttgtaa gtgatgttgg
    20221 tagtgtatta ataggaaaag aagctgtgga ttatggtctt atagatgaag ttggaggact
    20281 aaaagaagca cttaaaaaat taagagagct tataaaagaa tctgaggaag aaaaaaataa
    20341 tgattaaaat ttgtttaaat aataaaattt atgatataat gatgtaataa agtaggagtg
    20401 ttagtgttaa ggcattagca ctttttgctg tgaaggggta aaacttgcta aaaataagga
    20461 gtgatattaa tggccacgaa aaagaagaaa aaaaagaagg taaatacctt aagttttaat
    20521 gcagaatatc ataatttaat aacaatattt ataggtgtat ttttattata tagtttaaat
    20581 tctaattcta tgggatggat acctgtgtta atgcaaaatc tttttaaggg actatttgga
    20641 gggctttcaa tagcaatacc atttatagta attataacag gacttttagg attttttgat
    20701 ggaaatgaat atatatatag gctaagaaag actaaactat actacataat tattttattt
    20761 atatttgtat tttatggatt attaaatgca ggaactcttc ctgtagatag cccactaaaa
    20821 ggtaacatgt ttgatgatgt tatgaaattg ggtgtaagtg gacaaggtag tggtttgata
    20881 gcaacaacta ttgcgtatta tatgagtaaa atatttggta tagcaggtgg atggttaata
    20941 agtatcttcg cacttatact ttctgtaatg tttatattta atatttctat aaaagatttg
    21001 gtgtcaaatg caaaatcaaa atctaaagca tctaaagaca gcaatttaac gtttaaagat
    21061 aaaattgcaa atatgaaaaa atctgcaata gatatgatga ctgatgaagt agatgatact
    21121 actataaata aaaaaccagg tttctttaaa gggttaatgt caaaaggtct taataatgat
    21181 gatgaagaag atgaatattt tgaagaagaa aatagtgatg gtgtagatga taaaactata
    21241 aaaatagttg gatttaataa ggcagaagat gagtatttag aaatattaga aggaactcaa
    21301 agtatgccag agttagatgt tttaaaagaa ttacaaaaag ctaccaatga aaatccagtt
    21361 gtagatacta aaccagaaaa aaaagttgat atagctaaat ctaatttaaa tatagaaaaa
    21421 actcaaccta tgagtattgt agcagagcca gttaatgagg attattcaaa ctataaaaag
    21481 cctagcattg agcttttaaa taaagtaaat aagaagtctg atgaaaatgg taaaaagaaa
    21541 gtgttgaaaa atgcttctct tttagaaaag acactatcag attttggagt ggaagcaaaa
    21601 attaatcaag ttacagttgg acctactata acaagatatg aaatacaacc aagtccagga
    21661 gtcaaggtaa gtaaaattgt aaatttaact gatgatatag ctttaagttt agcagctaaa
    21721 agtataagaa ttgaagcccc aattcctggt aaaagtgcaa ttggaataga agttccaaat
    21781 gaagaagctc aaatggttgg tgtgagagaa gttctagaaa gtgaagaatt taataatttt
    21841 aactctcctc ttgcaatggg acttggaaaa gacgtagcag gaaaaattat aattggagat
    21901 ataggtaaga tgccacactt actaatagct ggttctacag gttcaggaaa gagtgtgtgt
    21961 gtaaatacat taataagtag tattttatat aaggcaaatc ctgatgaagt aaaattacta
    22021 ttaatagacc caaaagtagt tgaacttgca aattataatg gaataccaca tctattgata
    22081 ccagtagtta ctgaccctaa aaaagctgca aatgctttaa attgggcagt tacagagatg
    22141 aatagaaggt ataaattatt tgcagatgct caagtaaaag atgtaacaag ttataatgag
    22201 aaagcggaag aaaaacttcc taaaatagta ataataatag atgagttagc agatttaatg
    22261 atggcaagtg caaatgatgt agaagattat atttgtagat tagctcaaat ggcaagagct
    22321 gctggaatgc acttgattgt agcaactcaa agaccatctg ttgacgttat aacaggggta
    22381 ataaaggcta atataccatc tagaatagca tttgcagtat catctcaaac tgattcaaga
    22441 acaatccttg atatgggtgg tgcagagaag cttctaggta aaggtgatat gttattctat
    22501 ccacttggtg cagcaaaacc agttagactt caaggggcat tcatatcaga gagtgagtcg
    22561 gaaaaagtaa ttgattttgt aaaaagtcag gttaaagatg gaataaaata tgaagaagat
    22621 ataattgaaa ctatatctaa ggttaatact tcaaagagta gtgatgagga tgagtttttg
    22681 agtgaagcga tagaatttgt cgtggaaagt ggtcaagcat ctgcctctat gctacagaga
    22741 agatttaaaa taggattcaa tagagcagct agacttatag attctatgga agaaagaggc
    22801 ataattggag caagtgaggg aagtaaacct agaaaggtat taataagcaa acaggatttg
    22861 cagaatttag aaggtgaata gaattgtcag ttataaaaat agaaaaagca cttaagctag
    22921 ataaagtaat atttgtagat gtgagaactg aaggagaata tgaagaagac catatattga
    22981 atgcaatcaa tatgcctctt tttaaaaata atgaacataa tgaagttgga acaatttata
    23041 agatgcaggg aaaacatgaa gcaatacaaa aagggtttga ctatgtatcg tataaattaa
    23101 aggatatata tttacaagca gcagaacttg cattaaatta tgataatatt gtcatatatt
    23161 gtgcaagagg cggaatgaga agtggaagta tagtaaattt attatcatca ttaggtgtaa
    23221 atgtatatca acttgaaggt ggatataagg cttatagaaa ttttgtatta gagtatctaa
    23281 ggcatgtaat ggatacaaaa aactttgtat ctgttcatgg attgactggt acaggaaaga
    23341 ctgatttgtt acatttatta gatgaaaaag gaatagatgt actggattta gaaggcatag
    23401 ctaaaaacag tggttctacc tttggtttta taacttttga taaaaagcca ccatctcaaa
    23461 agaactttga aacaaaacta tttgaaagtg tatttttttc gaaagagaat tatatatttg
    23521 tcgagagtga aagcaaacga gttggaagtg ttacagttcc aaatgaaatt ttcgaggcaa
    23581 tgacacgaga aggacatcat atattattag aatgtgatat tgaaaataga gttgatagac
    23641 tttgtagaga ttatatttat gataaagatg aaggtaatat attgatttta aaagaatgta
    23701 taaacaagtt tagaaaaaga ttgggtaata aagtagtaga tgattatatt gatttattag
    23761 aatcaggtga atataaagaa cttgtaaaaa gatatctttt agaatactac gacccacttt
    23821 atatgcattc agtagagaaa tataagtata gtaagattat aaattttaat gatacacaaa
    23881 aagctttaga agatgttata gacttatatg agagtatatt ggaaggagaa agtgaatgtt
    23941 aaaaatagca ttggaatcat taggatgctc aaaaaattta gtagatgcag aaataatgat
    24001 gggaatttta aataacaaag gctataaatt aattggagat tttgaagaag ctgatgtaat
    24061 tatagtaaat acatgtgggt ttatagagtc agctaaacaa gaatctatag atacgataat
    24121 aaacttttca gagttaaaaa aaactggcaa tttaaagttg ttgatagtaa cagggtgtct
    24181 agcccaaaga tattctgaag aattaaaaac tgaaattcct gaaatagatg ctatagtagg
    24241 aacaggaagc tatcaaaata tagataaaat acttaaagaa ttaagtgaaa tgcatcaaat
    24301 tgtaagcctt aatgatatag agtttgtatt taatgaagat ttaccaagat atatatctac
    24361 acctagctat atggcctatt taaaaattgg agaaggatgc tccaataatt gtacttattg
    24421 tataatacca aaactaagag gtaaatatag aagtagaaaa tttgaagata taattaaaga
    24481 agctaagaaa ttagctgaat ctggagtaaa agaacttgtg gttatagcac aagatacgac
    24541 aaagtatggt tttgatttat atggaaaaga aagattatca gaacttttag aagaacttgc
    24601 aaaaatagat ggatttaagt ggataagggt tatgtattca tatccagaat ctataacaga
    24661 ggaattgata caagttataa agaaatatga taatatatgt agttactttg atatgccaat
    24721 acagcatgca agtaacaata ttttaaaatt aatgaataga aaaactacta aagaagatat
    24781 attaaataaa atcaatctga taagaagtaa tataccagat gctatactta gaactacgat
    24841 aatagtaggg tttcctggtg aaacagaaga tgacttcaaa caacttgtgg actttgttga
    24901 agaggttaaa tttgatagac ttggtgcatt tgcatattca agagaagaag atacaccagc
    24961 agatagattg ccaaaccata tagatgaaga agtaaaaata caaagaagag atactttaat
    25021 gatgattcaa caaaaaatat ctgaagaact taatgacaaa aagataggaa aaacttatga
    25081 ggtgttaata gaagaacaga tagaagataa tgtatataca ggaagaacac aaggtgatgc
    25141 ggaagaaatt gacagtatag tgtatgtaaa aagtgtagac aatcttgaag taggagaatt
    25201 tgtaagtgtt caaataaatg atgcaatgga atacgattta atgggagatg ttttatatga
    25261 acttgcctaa caagttaact ttgtttagaa tatttttaat accagtattt gttttaatta
    25321 tgttgctcaa tgtaccaaat aaatttttaa tagcttgtat tatttttata atagcttcaa
    25381 ttactgatgc tttagatgga aaaatagcta gaaaatacaa tctagtcact gactttggaa
    25441 aatttatgga cccattagca gataagttat tggttatttc agcacttaca tgtatgatag
    25501 aagaccatct tgtaagtagt tggatggtaa ttataatagt ggctagagag cttacagtta
    25561 gcatattaag agctatagct gctgctgatg gaaaagtaat tgctgctgga aacagtggaa
    25621 aacttaaaac gataactcaa atggtatcta tagtgttttt attgttaggc gctcagtttg
    25681 aaaatgtatt gatattaaat attggtgaaa ttctcattct tatagctaca ctacttacat
    25741 tatattctgg atgggaatat ctatataaaa ataaagagct ttttatgtct tctaagtaaa
    25801 aaattatgct ttaataaata aaaactaatg attaataaat aaaaaataac aacttaaaat
    25861 tttaaaaatg
//
LOCUS       DAEPOG010000038        25635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEPOG010000038 DAEPOG010000000
VERSION     DAEPOG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.038"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            99..2753
                     /gene="adhE"
                     /locus_tag="KR432_001336"
                     /gene_synonym="adhC"
     CDS             99..2753
                     /gene="adhE"
                     /locus_tag="KR432_001336"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0639882.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3029..5248
                     /locus_tag="KR432_001337"
     CDS             3029..5248
                     /locus_tag="KR432_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0639883.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5502..6164
                     /locus_tag="KR432_001338"
     CDS             5502..6164
                     /locus_tag="KR432_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639884.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6534..7943
                     /locus_tag="KR432_001339"
     CDS             6534..7943
                     /locus_tag="KR432_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBH0639885.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8058..8333
                     /locus_tag="KR432_001340"
     CDS             8058..8333
                     /locus_tag="KR432_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBH0639886.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            8963..9274
                     /locus_tag="KR432_001341"
     CDS             8963..9274
                     /locus_tag="KR432_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0639887.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9276..11201
                     /locus_tag="KR432_001342"
     CDS             9276..11201
                     /locus_tag="KR432_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBH0639888.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11204..11695
                     /locus_tag="KR432_001343"
     CDS             11204..11695
                     /locus_tag="KR432_001343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBH0639889.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            11747..12310
                     /locus_tag="KR432_001344"
     CDS             11747..12310
                     /locus_tag="KR432_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBH0639890.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12325..13302
                     /locus_tag="KR432_001345"
     CDS             12325..13302
                     /locus_tag="KR432_001345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBH0639891.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13289..13612
                     /locus_tag="KR432_001346"
     CDS             13289..13612
                     /locus_tag="KR432_001346"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBH0639892.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            13729..15507
                     /locus_tag="KR432_001347"
     CDS             13729..15507
                     /locus_tag="KR432_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBH0639893.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15504..16877
                     /locus_tag="KR432_001348"
     CDS             15504..16877
                     /locus_tag="KR432_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBH0639894.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            16883..17551
                     /locus_tag="KR432_001349"
     CDS             16883..17551
                     /locus_tag="KR432_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBH0639895.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            17828..18877
                     /locus_tag="KR432_001350"
     CDS             17828..18877
                     /locus_tag="KR432_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0639896.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            18973..19962
                     /locus_tag="KR432_001351"
     CDS             18973..19962
                     /locus_tag="KR432_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0639897.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            19967..21685
                     /locus_tag="KR432_001352"
     CDS             19967..21685
                     /locus_tag="KR432_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0639898.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(21775..22920)
                     /locus_tag="KR432_001353"
     CDS             complement(21775..22920)
                     /locus_tag="KR432_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0639899.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            23419..24780
                     /locus_tag="KR432_001354"
     CDS             23419..24780
                     /locus_tag="KR432_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0639900.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            24875..25579
                     /locus_tag="KR432_001355"
     CDS             24875..25579
                     /locus_tag="KR432_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0639901.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
BASE COUNT         9841 a         3005 c         4615 g         8174 t
ORIGIN      
        1 aaaatgttaa aaaaataact tgaaaataga aaaagtaaat gctataatgt taataaatta
       61 acataataaa taaaaaacta tctcaggagg ttgttaaaat ggctcataaa gaacaagtaa
      121 taatagaaaa taaaaaagaa aatgaagtta tgtcagttgg tttagatact gtaaataacg
      181 ttgaaagctt agttaaaaaa ctagcaaaaa tcagagaagc tcaaaaaata tttgctacat
      241 atactcaaga gcaggtagat aaaatttttt tagcagcatc tttggcagct aataagcaaa
      301 gagttcctct agctataatg gcacaaaagg aaactggtat gggaattgct gaagataagg
      361 ttataaagaa tcattatgcc tcagagtata tatataatgc atataaagaa actaaaactt
      421 gtggtgttat agagaaagac gaagcatttg gtatgactaa aattgctgag ccaataggtg
      481 ttattgcagc agttgtacct acaactaatc caacttctac tgctatcttt aaagcacttt
      541 tagctttaaa aactagaaat ggaataattt tctctccgca tccaagagca aaaaattcta
      601 ctatagaggc tgcaaaagtt gttcttgagg ctgctgtttt agctggagct cctgaaggca
      661 taataggatg gatagatgag ccatctttag aattaactac aactgttatg aaagaagtgg
      721 atttgaccct tgctacagga ggtcctggaa tggttaaatc agcatattca tctggaaaac
      781 cagctattgg agttggtgca ggtaatactc cagcaataat tgatgagagt gcagatataa
      841 aaactgctgt aaactcaatt cttgtatcta agacttttga caatggtatg atatgtgctt
      901 ctgaacaatc ggttatagta ttagaaaata tatacaatga agtcaaaaaa gaatttaaag
      961 agcgtggtgc ttatttatta gataaagatg aaacagaaaa aataagaaat ataattttag
     1021 taaatggagg attaaattct aaaatagttg ggcaaacagc ttgtactata gcaaaacttg
     1081 caggctttga agtcccagta gatactaagg ttttaatagg tgaagtagaa tcagttgaaa
     1141 tagaagaagc ttttgctcat gagaaacttt caccagtact tgcaatgtac aaggccagtg
     1201 attttgataa tgcagttaga aaagcagaaa aattagtaga agatggtgga tttgggcata
     1261 catcttcatt atatattgat gatgtaaatc aaagagaaaa acttgataag tttacttcag
     1321 ccatgaaaac ttgtagaatt ttaattaata ctccttcttc tcaaggtggt ataggggact
     1381 tatataattt taaactagct ccttctctta ctttaggatg tgggtcttgg ggtggaaatt
     1441 cagtatctga aaatgtagga gttaagcatt tacttaatat taaaacagta gctgagagga
     1501 gagaaaatat gctttggttt agatcaccag gaaaagttta tttcaaaaaa ggttgtttag
     1561 gggtagcatt aaaagaactt aaagatgtaa tgaataagaa gagagctttt attgtaacag
     1621 atacattcct ttacaataat ggatatacta aagcagttac tgatttatta gatgaaatga
     1681 atattaagca cactacattc tttgaagtag aaccagaccc aactttggaa tgtgctaaga
     1741 taggcgctaa agctatgaga gaatttaatc cagatgtaat aatatctata ggtggaggaa
     1801 gtgcaatgga tgcaggaaag ataatgtgga cattgtatga gcatccagat gtagatttcc
     1861 aagacctagc tatgagattt atggatataa gaaaaagggt atatacattc cctaaaatgg
     1921 gtgaaaaagc taattttgta gctataccaa catcagcagg tactggttca gaagtaacac
     1981 catttgctgt tataactgac caagatacag gggtaaaata tccattagca gattatgaac
     2041 tgatgccaaa tatggctata gttgattctg atatgatgat gaatatgcca aaaagcttaa
     2101 catctgcttc tggtatagat gcattaactc atgcattaga agcatatgta tctatgcttg
     2161 caactgatta tacaaatgga ttagctttac aagccattaa gagtatattt gaatatcttc
     2221 cacgtgcata tgataatggt gcaaaagacc cagaagctag agaaaaaatg gcaaatgctt
     2281 ctacaatggc aggtatggct tttgcaaatg catttttggg tgtttgtcac tctatggctc
     2341 ataaattagg tgctttccac catgtaccac atggtgttgc aaatgctctt ttgataacag
     2401 aagttatgaa atttaattct tcagatgcac caaagaaaat gggagcattc tctcaatata
     2461 aatatccaga agcattaaaa agatatgctg aaatagcttc atttttagga ttaaagggaa
     2521 attctgatga agagaaattc caaagtttat tggtagctat tgaagattta aaacttaaag
     2581 ttggtattcc taaatctata aaagagtttg gagttgaaga acctaagttt atggattcaa
     2641 tagatgaaat ggtgattcaa gcatttgatg accaatgtac aggagcaaat ccaagatatc
     2701 cacttatgaa tgaaataaaa gatatgtatt taaattcata ttatggaaga taaatttact
     2761 ataaaataga taaaataaaa actcttatac ataaaacaaa gttttattgt ataagagttt
     2821 ttttgtttag aaaaattgat agtttttatt ctttaattta aaattcaact aaatagtgtt
     2881 attatgataa taaaagaatg tttttatggt tattaggttg attttatgtg taacatttaa
     2941 gaatagttat atacaatata aatgatgaat cggtataaaa aggatatatt atctatatga
     3001 agtattgact aattagggga ggagtcgaat gacagggaga aataaaagtt ttacattaat
     3061 tacaattctt tttttaacat cattattttt gacaatgggg tatatctata tagaagatac
     3121 aaaacatctt cttatgtcag aggcaaaaat acatataaaa gaagttgcag tacaaggctc
     3181 tcaactagca caaagacaaa ttgaaaaaga tttagatgtt ttaaatattt tttctaacta
     3241 ttatgcttta aatcaagata tacctaatga ggaaaaaatg aaaaatcttt tagatgaaat
     3301 ggaaaatcaa aaattttaca ctatggcaat agttgatata aatggtaatg ctgaaaatac
     3361 caaaggtgat aagttttcag tgaaagatag agaatttttc aaaaactcaa ttaaaggtaa
     3421 gaagtatgtt tcaagtccat atgttgatga agtaaataag tctataaaga agattactat
     3481 ttcagtacca ttattaaata atgataaagt agttggagtt ttatattgta cttacaatat
     3541 caatactctg atgaaactta ttaatgtatc tttttatgaa aataatagta tatcttatgt
     3601 tgtaagaaat gatggtacaa ttatccttca tccgcaagag gatagcctat caaaaaatat
     3661 ttataaattg ttaaaacagg ataatgatat tcgagaaata aatagattaa aaaaagaatt
     3721 gcaagaaaat aaaacaggtg caactgtatt aaatatgttg gaagaaagaa gatatttagg
     3781 atatgctact atggataatg ggaatagtga aaataattat ataaaagatt ggaatttgat
     3841 tttttcaatt ccagaaactg ttgtatttag taattcagaa caaataatca acagagctat
     3901 atatgcagtg ttatttatta tattaatatt tattgcaatt attttttata taatttatat
     3961 aaaaaaatcc aatgaaaaag aaatattaag tttggcttat gaagataaag ttacccatat
     4021 aggaaatcaa aataaatttt atagagagtg tagtaagtat cttttagata aaccatcttt
     4081 aaattatatt atagtatatt ttgatattaa taactttaag atgattaatg atacttttgg
     4141 atatgaattt ggtgacaatt tattaattac gatagcaaaa gctttaaaag aagaattaac
     4201 agaaggtgaa gtatatgcac gactttcaag tgattatttt gcaattttct gtgattataa
     4261 aaatggaaaa aataaacgta taagaaaaat tgatagtata cgaaataata ttgaaagtaa
     4321 tttgagtata gtatttgaaa tttctctgtg tgtgggtatt tattttgttg aagaaggtga
     4381 agtagatatc caaaaggctg taaataaagc aaatatggct aggtctgttg ctaaaggtaa
     4441 aaatataaat tatgctattt ataatgaaga tgttaggaat aagcttagtg aagaatcaat
     4501 gattttagat gatataaaaa tagcattggt aaaaaatcaa tttgaagtgt attatcaacc
     4561 taagttttca cttgtaactg gagaaatgat agggagtgaa gcactaataa gatggaatca
     4621 tccagaacat ggttttataa gtcccgcagt atttattcca atagcagaga aaagtaagtt
     4681 gattttaaaa attgggagat ttgtatttga aagggtttgc aatgatttat ctgaatggaa
     4741 aaagcaaggt aaaaagatag ttccagtttc aataaattta tctagagttg agttgtatca
     4801 accagatatt gtaaaattta tcaataaaac aatacaaatg tataatctaa gttcagattt
     4861 aattgaaata gagataacag aaactgtggc tattaatgag ttaaatattt taaagaatgt
     4921 tttaaatgag ttgaggaagt atggattttc tatttctatg gatgactttg gtacaggata
     4981 ttcttctatt agttgtttaa gagatatgcc aattgatata ttgaaactag ataagtcttt
     5041 tcttaatggt attgaacatg atgagagaag tcgcaatata gcaaaatcaa tagtgtctct
     5101 agcaaaatca ctagatttag ttgtaattat agaaggtgtt gagagtaaag aacaagctga
     5161 gttaatgaaa caatttggat gtgatttagt tcaaggattt tattttgcac gaccaatgcc
     5221 agctaagaat tttttagatt tactttaaaa caaaatttaa tttttatata aagcactatt
     5281 atgaatgttt gattttaata aatcactgct aaataattag tgttttttta ttttatcaat
     5341 gtattgttta atataaagca aaaaataatt gtattttata aaaattatat taaaaataaa
     5401 tttattttgc taataaacat gttattaaca aaagaatagt actataatgt atagatgata
     5461 aaaactttaa taaaaattta gtgtatagga gttgtttaag tatgatagat gtgagtattg
     5521 atgacaaact taaggaaaga tggcctaatg ctaagttggg ttgcattcaa gcaaaagtag
     5581 cagtaaataa aagctcagaa aaattaatta atgaaataaa tgagttttgt gatactataa
     5641 atcaaagtct aaaagttgaa gacattacga aactagataa aattaaagat gcaagaaatg
     5701 cttataaaga gcttggaaaa aatccaagta gatatagaac atcttcagaa gcattggtta
     5761 gaagaattat tcaagggaag ggtctttata caataaataa tatagtagaa ataaacaatt
     5821 taatatcaat aaaatcatta tatccagtag gtacatataa tgtaagcaaa ttacattcac
     5881 cagtatgttt tactgtagga gatgaaggag aacagtataa gggtattggg aaagaattaa
     5941 taaatataga aaatcttcct atattgtctg attcagtggg taaatttggg agtccaacta
     6001 gtgattctga aagagcaatg ataacagaag atgcaaatga aatattaatt tgtatatttt
     6061 cttttagtca agaatctgat attgaagaat attttggata tgctaaggag ttacttaaag
     6121 aatatgctga tggaaaagat atagaaacta aaataattaa atagaatcta tataacaacg
     6181 ttatgttaag ctaaaattca atataaaaat aagttaagat aaagaaacta acataaaatt
     6241 agtttctttt ttaaaagagt agtttagtgt tacaaaaaca gatagataac ccacgattat
     6301 taaaagtatg gtaatatatt aatatagcag agattgtatt taagggattt tgaaaggggt
     6361 gatttataat agtcattttg tctaaaagat aaaatggttt tatggaaata actatatatt
     6421 taaaaatata aacaatgata cagtaatttg ggtatataaa ataaggcata catggttttg
     6481 tttaaaaacc aataaagtac ataggtaatt acaatgtaag gaaggattta attatgattg
     6541 atggaaaaga ggtaaaacaa gaaagtggac aaaaaaatct aaaatttaag atagctatag
     6601 ttgtaggtgt tttgtttgta gcttatatag gaatggcaat cttttttaga aatcattttt
     6661 actttggaac gactattaat ggcgtaaagg cttcaggaaa aacagttgag caagtaaatg
     6721 atttgttaac atctagtact aattcctata ctttaaactt agaggaaaga aacaataaga
     6781 aagaacaaat aaaagcaaaa gatattgatt taaaatttac accagatgat aaagttcaaa
     6841 agataaaaga tagtcaaagc gctattggat ggattggtgg aatatttaaa tctaaagagt
     6901 ataataatat gataactctt tcatataata aagatttact agaaaaaagg tttaatgctt
     6961 tatcttgttt tgatagtaaa aatgttgtag aacctaagag tgcttatcct aagtatgata
     7021 gcactaaaaa cgcttatgtt atagtaccag aggtacttgg aaacaaagtg aaaaaagatg
     7081 atttttataa gttagtacaa aactcaatta attctgggaa tacttctatt tctctggatg
     7141 aaagtaaagt ttatgaagca ccaaacaaca caaaggattc agaaaagttg aaacaagcta
     7201 ttaagacatt agataagtat gttggtgtag tcgttacata tgattttagt gaccgtaagg
     7261 aaactttaga tggttctgaa attaataaat ggttaaaagt agactcagaa aagaattatg
     7321 acataacttt tgataatgat gctatggtag aatatacaag aagtttaagt aaaaaataca
     7381 gtacttttgg tgactcaaga ccatttaaaa ctgccacagg taaagatatt acaatttcag
     7441 gtggaattta tggttggttg atagataaaa agaaagaagc tgaagcatta gttgatgttg
     7501 taaaagctgg taaaaatgtt acaagagaac ctatttacat acaaaaagct gtatctcgta
     7561 ataaagatga tattggaaat acatatgtag agattagtct ttctggccaa catatgtggt
     7621 tctttaaaaa tggagaaatg cttgtatcaa cagatgttgt aacaggcgat ttagctagag
     7681 gatttgctac accatcaggt atatatcctc ttaattataa ggctagaaat gtttcattaa
     7741 ctggtcaagg ttacagttca cctgtaagtt attggcttcc atttaatggg aacataggca
     7801 ttcatgatgc tacttggaga aattcttttg gtggaggtat atataagtct tcaggttcac
     7861 atggatgtgt aaatacacct ttttctaagg caaagaaaat atatgaaaat atagaaccag
     7921 gtacaccaat tattttatac tagtaaatgt gttgctaaca gttaagcgaa gtcatcttag
     7981 tttatttagg atggcttcct ttttatataa agcttattga agtttacaga aagactactt
     8041 aaaaggagaa atacataatg aatgaaaaat taactaaaat tttagaagaa gctaagaatg
     8101 atgatgaatt tagaaaggaa cttataaaaa caagagaaca acgtgatgtt atggattcat
     8161 tttgtaaggt tgttacatct agaggttatg atataacagt aggggaactt ttttccatgg
     8221 gagaagaata tacatcaaac ctactgaaaa gtgtaaatgg aggagcagca tatcctattg
     8281 aagattggag tgattggtac gaggattttt ttataatgtt gtcgaatata tagagtaact
     8341 aatgatatta tctacttaag attaatacat tgccttaaaa taaaactatt agaactaagt
     8401 gtaagtttta tcaatcttta aaaatattga tttatttcta actatatata aaaaatttag
     8461 attgaaaata aggtgacaaa aatcgatata gcttgatgat ttataaaagt tagataaaat
     8521 agtgcgagat atttctaaaa attcaaaaaa aacaataaac ttcaagggtt agaggttgaa
     8581 aaagaatata aaaaactttt atatgtaata aattaaatta aaattataaa aaaaataaaa
     8641 taaaaattta aaatgtcgac atagcgttaa ttttaaaaca aaataacgct attgacaaac
     8701 tatatcgctt taaattataa ttattgaaaa taatggtatt taaaaaccat actcatgtca
     8761 tagtctaaaa gtaagtcaaa aaaggatgta aatctaggca agtactattc taaaatatgt
     8821 tttattaaga aataaacgtt gaaaaatatt aattattata taaaaatgct gtttatactt
     8881 taacaatgag gtagtaatct aattagaata aaaaaatcta attggttata tacatagtaa
     8941 aaaggtaaga gaggtgattt ggatggcttt tgaaataata aaaagcattg ttgaggcaga
     9001 gcagacagca gacagtatca aagtaaaagc tgttactgat gcagagtcta tcagagctga
     9061 tgctgtaaac aaatgtgaaa gcatatttgc tgatgtaaga aaacaagcaa agcttatgga
     9121 agaaactctt attgagaagg cagtcaccga cagtagagca gaggttgata aaatcttagc
     9181 taatgctaaa agtgaatgtc tgaaaattga aaaaactgct gaagaaagaa aaagtaaggc
     9241 tattgaagcg gttattggaa aggtagtgag ataatatggc aatagttaat atgaacaaaa
     9301 tatctattgt tggcttagaa gctcaaaaaa gccagatttt aaagctactt atgaaccgtg
     9361 ggtttgttca gattgatgat agtgcctttc ttactgagga ggaggaatta aaaaattatc
     9421 ttgtaaaaga tagtgaagaa tctgaagtta taatgctaga gcagaaaatg tctcatgtaa
     9481 accaagcaat taatattgtt tcttcactag tgaaacaaaa aaaatcccta cttgcaccaa
     9541 aaagagagtt taacaaaatt gatagtgaaa aagaagggca agtttatgct gaagctctgg
     9601 agttaaacaa tttgcacaag gagataggta caataaggtc taacataaac ttacttaaca
     9661 acaataaaag tatgttagaa ccatgggtga actttgatat tcctcttgaa aatatggaaa
     9721 ctaagtatac aaaaactata cttggaactg tgcccataag cgcaaatatt gatgctttaa
     9781 gagtaaaatt agaagatgaa ataccagaaa gtgttattgg gattgtcaat tctgacaagt
     9841 taatgaacta tgtttattta attactttaa aagaaacttt tgatgatgtt ttagaagttt
     9901 taaaagaatt taatttttca gtggtttcat taccaaatga agaaggtaca ccgttacaag
     9961 ctatcaagaa atacaatgaa atgataatta ggcaggaaaa cgtttctaag gacaaaacag
    10021 aggaaattaa aagacatgca gatagtatac acatgcttga aaacctatat gatttttaca
    10081 ctatagaaag agaccaaaaa aagattatag agagacttgt caaaacaaaa acaacattct
    10141 gtcttaatgg ttggttacca agcaaaaggg cagatgaatt aattaaagaa attacagaaa
    10201 agtttgattg ttgtgttcag actgaagaag gaagtaaaga agaaggcttc ccaattctgc
    10261 ttgaaaacaa taaattagta acaccttttg aaagtgtaac taacatgtac agctatccaa
    10321 gtacaaaaga tattgaccca aatacaattt taactttctt ctttgttgta ttctttggaa
    10381 tgatgctaag tgacgccgca tatgggttaa taattgcagt agtctgtgga tttgttgtat
    10441 ataaattaaa gatacaaaaa ggtgaaggaa atttaattaa acttattggt atctgtggag
    10501 tttcaactac tgtatggggt cttatattcg ggggtatttt gggggattta attcctataa
    10561 aagcattaat taatccttta gaggatgtta tgacacttat gggaatgtcg ttactgttcg
    10621 gtattatcca tatatatgta ggtttgggaa taaaagcata tacattaata agaagtggaa
    10681 aggtaataga tgcaatcttt gacataggtt tttggtattt gtgtataaca ggtgtatgtc
    10741 tacttataat accttttgtt gcaggggaca taggtgtatt ttcagaagta ggaaaatatt
    10801 tagcaattat aggtgctata ggtcttgtac ttactcaagg aagaagcttt aaaggtattc
    10861 ctgttaagct ttttaaagga ttttcaagtc tctatggcat aacaagttat tttgctgata
    10921 tattatctta tacaagaatt atggcgcttt gtttaacaac aggagttata gcacaagtta
    10981 tcaacctttt aggggctata gctggtccga tacttgcagt tgtaatcggt gttgtaggac
    11041 acacaataaa ccttcttata aatgcactag gtgcatatgt tcatacaagt aggttacagt
    11101 atgtagaatt tttcaataag ttttatgaag gcggaggagt tccttttgtt ccgtttaagt
    11161 ataaaacaaa atatacaagc attaataaaa aggagatgta aatatggaat ttttaaacgc
    11221 gttaggaaat ggtcaatttt tggcaatatc aggagctgct atagcagcat tatttgcagg
    11281 tatgggttct gctaaaggta cttcaatagc aggtcaagcc gcagcaggtg ttgtaacaga
    11341 agaccctagt aaattcggtc aacttctagt tttacaactt cttcctggta cacaaggtct
    11401 gtatggattt atcatagctt tccttatttt atcaaaagca gggattattg gtggagatgc
    11461 aataccaaca tcagctcaag gtttacaact tcttatggca ggacttccaa ttggtttagt
    11521 tggtttagta tcaggtatag cacaaggtaa agcagcagca gcaggagttg gaatagttgc
    11581 aaaaagacct gaagaacttg gtaaagctat aacatttgca gcaatagttg aaacatatgc
    11641 tcttttagga ctacttgttt ctttcttagc ttacaatgga attgcaattg gttaattata
    11701 aattataatt cataagtaaa aaaaatgaaa aggaggaagc actcctatgg gtaatgaaca
    11761 aaaaatgatt gacagaatta tagctgatgc caaacaagaa gcacaagaaa tattggataa
    11821 agctaaaagt gaagcagatt taaaagtaaa ttcagctaat gaaaaagctg aaaaggaaat
    11881 ggcttcctat acaaagcttg cagaggcaga agcagaaaaa gcggcttcta aggaaatatc
    11941 aggagcttat atggaggcta aaaagcaaat attatcaaaa aaacaagaga ttttagaaga
    12001 ggtaatttta gaagctaaaa acaaactttt aaatcttaaa gataatgaat atgaagagat
    12061 tatactaaac atgattgaaa aatcaaattg tacagatgat tcagagatag tattatctaa
    12121 aaaagataaa aaaacattaa aagatgtgct atcaaaaaaa ggcattaaag tgtctgatga
    12181 aactagagat attactggag gtttcatagt taaaaaaggt gacattgagt acaattattc
    12241 atttgaagcc ataattgcag tagagcatga atatattgaa caaattgcag cagaaatttt
    12301 gtttaattaa ggaggggtta aaaaatggca gaagaaaaaa cttatcccta tgccgttgca
    12361 cgaataagag tgcttgaaaa acagcttctt aacagacaaa tgtttattca aatggcagaa
    12421 gccaaaagcc ctgaagactc tttaagaatt atagctgagg caggttatgc tgatacatca
    12481 aataataata tacataattt tgagaatatt ttgactcaag aattatcaaa gacatatgaa
    12541 attcttaaaa gtctagctcc tgaagaaaaa tttgttgatg tatttcttta taaaaatgac
    12601 tatcacaatt taaaagtgct gattaaagaa gaaatttctg gtgtagatgg tgagaaatat
    12661 ttaattgatg gaggtacaat tcctcttatt aagcttagag aatctttagc aaatcgtagt
    12721 tttagtgatt tacctaagat aatgtcatca gctatattag aggcatttga tgtttataat
    12781 aagacacaaa atggccagat ggttgatatt gttctagata aagcaacctt taccagtatg
    12841 aaagaaactg ctaaagaaag ccaaaacaaa tttgtaatag attatgttat gaaagtttgc
    12901 gatttaacaa atctaaagag ttttataaga gtcaaaaata tgaagaaaga ttttgatatg
    12961 tttatgaatg tgtttgtttc aggtggctca cttgataagg aaaaattctt agaagctttt
    13021 tcctcagata ctccagcatc atgttttaaa tctacttctt actctgatgt ctgtaaaaat
    13081 ggcatggata gtggttttac tgtctttgaa aaactttgtg atgactatct tatggaatat
    13141 gttagaggag caaaatttaa gcctcttaca ttagaaccac taattgcata tttatatgct
    13201 aaagaatcag agataaaaac aattagaatt attttgacta gtaagcttaa taatatagac
    13261 gcagatacta taaaagaaag gctaagggat gcttatgtat aaggtaggag tagttggaga
    13321 taaagatagc atcatgggat ttttagctct tggaatagat atcttcccag cctatgatag
    13381 tgacgaaatt aaaaaaagta tacataagtt ggttgaagat gaatatgcga taatttatat
    13441 tacagaacag gcatcattgc ttgcaaaaga atcaatagca aaatacaaag attaccaact
    13501 tcctgccatt atagttattc caggtattgg aggaagtatg ggattaggta tgaatgaagt
    13561 aagagaatct agtaaacgtg ctataggtgc agatatatta tttaaagaat agatgaaagt
    13621 tgaataaaag acaatctcaa attttattgg tgctaaagat atgtgctaat aaaatatagg
    13681 agggaatatt taaattttga taaagtatat gattgcaggt gagagaatat gaaaacaggc
    13741 acaattgtaa aggtgtcagg accgcttgtt gtagcggagg gcatgagaga tgcgaatatg
    13801 tttgacgtcg taagagtttc agataaacat cttataggcg agattataga aatgcatgga
    13861 gataaagctt ctatacaggt ttatgaggaa acttccggtc taggaccagg ggaagaagta
    13921 gtttctcttg gaatgcctat gagtgttgaa cttggaccag gtcttatttc aactatttat
    13981 gatggtattc aacgtccact tgaaaaaatg tatgatattt caggtactaa tataactaaa
    14041 ggtgttgaag tatcttcact tgatagaaca actaagtggg aatttaaacc taaaaaatct
    14101 gttggtgata aggttgtagc aggagatata attggtggcg ttcaagaaac agcagtagta
    14161 gaatgtaaaa ttatggttcc ttatggagtt aagggtacta taaaagaaat atattctggt
    14221 gaatttactg ttgaagatac agtttgtgtt attacagatg aaaaaggtaa tgatatacct
    14281 gtaactatga tgcaaaaatg gccagttaga aaagaaagac catatagaga aaagaaaaca
    14341 ccagattcac ttcttgttac aggtcaaaga gttatagata catttttccc aatcacaaaa
    14401 ggtggggtag cagctatacc gggacctttt ggaagtggaa aaactgttac acagcatcag
    14461 cttgctaaat gggcagatgc agatatagtt gtatatatag gctgtggcga gcgtggaaat
    14521 gaaatgacag atgttcttct tgaatttcct gaattaaaag acccaagaac aggcgagtca
    14581 cttatgcaaa gaactgtgct tatagcaaat acatcagata tgccagttgc tgcacgtgaa
    14641 gcttctatat acactggtat tacaatagct gaatatttta gagatatggg atatagtgtt
    14701 gcacttatgg cagactctac atcaagatgg gctgaggctc ttagagagat gagtggtcgt
    14761 ttagaggaga tgcctggtga agaaggttat cctgcatact taggttcacg tcttgctcaa
    14821 ttctatgaga gagcaggaaa ggtaaattgt ctaggtatgg atgaaagaga aggaacactt
    14881 acagcaattg gtgcagtttc tcctcctggt ggtgatacat cagaacctgt cagccaagca
    14941 acgcttcgta tagtaaaagt attctggggt cttgatgcat ctttagcata taaacgtcat
    15001 ttcccagcaa ttaactggtt gactagttat tcactctatc aggaaaaagt tgatgtatgg
    15061 gcagataaaa atgtaaatga taatttctca gctcaaagag ctcaagctat gaaactttta
    15121 caagttgaat ctgaacttca agaaatagtt caattggttg gggttgattc actttcagat
    15181 ggagatagat tgatactaga ggttactcgt tctataagag aagacttcct tcatcaaaac
    15241 tctttccatg aagttgatac ttatacatca cttcataaac aatatagaat gatgggactt
    15301 atattaaaat tctataagtc agcacaagat gctatcaagc aaaatgttac tataaacgat
    15361 attatcaagc tttcagttct tgaaaagatt ggtcgtgcta agtatgtaga agaaggcgat
    15421 atagatgcag cttatgattc aattgaagat gaaatagaca acgagcttgc agaccttatt
    15481 aaaaagaggg gggctgaaga actatgataa aggaatataa gtcaatacaa gaggtagccg
    15541 gtcctcttat gttaataaac ggcgtagaag gtgtaaaatt tgatgaactt ggagaaattg
    15601 aactttcaaa tggagaaatt cgccgttgca aagttctaga ggtaaatgga gatactgcac
    15661 ttgttcagct tttcgaaagc tcaacaggta taaaccttgc tgaaagtaaa gttcgttttt
    15721 taggaaagag tcttgacttt ggagtttcac cagatatatt aggccgtgtt tttagtggaa
    15781 tgggtagacc tattgatgga ggtccagaaa ttataccaga taaaagactt gatataaatg
    15841 gagctccaat aaatccagca gcacgtgatt atccggccga atttatacaa actggagtct
    15901 ctgctataga tggacttaat acattggtta gagggcaaaa acttcctata ttctcagggt
    15961 ctggtttgcc acatgccaat cttgcagttc agattgcaag acaggcaaaa gtacgtggaa
    16021 ctgattctaa gttcgccgtt gtatttgctg cagttggtat aacttttgaa gatgctgaat
    16081 tttttataag cgattttaaa gcaactggtg caatcgaccg ttctgtagtg tttgtaaacc
    16141 ttgcaaatga cccagctgta gagcgtgtat ctacacctag aatggcactt acagcagcag
    16201 aataccttgc ttttgagcaa gatatgcatg tacttgttat aataacggat attacaaact
    16261 atgcagaggc attgcgtgaa gtttcagcag ctaaaaaaga agttccagga cgtcgtggat
    16321 atccaggata tctttatact gaccttgcta ctatgtatga aagagcagga aaaatgaaag
    16381 ggcatgaagg gtctataact atgattccaa tacttacaat gcctgaggat gataagacac
    16441 atccaattcc tgaccttaca ggatacatta cagaagggca aattatactt agccgtgaac
    16501 tttataaaaa agatatacag cctcctatag atgttttacc atcactttct cgtctaaaag
    16561 ataaaggtat tggtaaaggt aagactcgtg aagaccatgc agatacaatg aaccagcttt
    16621 ttgcagctta ttcaagaggt aaggatgcaa aagaacttat ggctatactt ggtgaatctg
    16681 cactttctga gattgattta atgtatgcta agtttgcaga tgaatttgag aaagaatatg
    16741 tttcacaagg atttagaact gatagaacta ttgagcagac attagaaatc ggatggaagc
    16801 ttcttagtat gttaccaaaa agtgaactta aacgtatccg tgatgaatat tttgaaaaat
    16861 atatgcctaa ggagtgatat ctgtggctag attaaatata aatccaacac gtatggaaat
    16921 gactagactt aagaaactcc ttaaaactgc tacaagaggt cataagcttc ttaaagataa
    16981 acttgatgaa cttatgaaac aatttcttga gattgtaaga gaaaataagc gattgcgtga
    17041 agaagcagaa aatgctcttg atactgctta taaaaacttt ataatcgcaa gagcagttat
    17101 gagtcaggag tatctgggtt cagcacttat gatgccaaag caatcagttt cagtagatgt
    17161 ttctacgaga aatataatga gtgttgatgt tcctgtgttt gactttaaaa ctgaaaacaa
    17221 tcaaagtgat atttatcctt atggtcttgc ttttacatca ggtgaattag attctgctat
    17281 ggaagctttc tcagatgcta tgcaaccact tttaagactt gcagaaagtg aaaaaagtgc
    17341 acagcttctt gcacaagaga tagaaaagac acgtagacgt gttaatgctc ttgaaaatgt
    17401 aatgatacca aattacattg agactattaa gtatatagct atgaagcttg aagaaaatga
    17461 gagagcaagt actactagac ttatgaaggt taaggatatg gtgcttaaaa aagcacttga
    17521 agaaaagaaa aaaaatgatt tggttgtata attaaatact tataaaaata agtctaacat
    17581 tctactttat agaggttaga cttatttttt atgtttaaat taaaatgtag atactataaa
    17641 aattaatata ttgtttaaaa acagactgta tttaactaaa aataatatat ttaacaaaaa
    17701 tttctaaaaa ttaacgaact gttaatttac acttaacttt atattaacaa attaggtgta
    17761 ttatataaac ataagcaaaa acaataaaaa acaaataaaa aaaggaaaaa acacaaaggg
    17821 ggaagatatg aaagaattta tatttacttg tctgtgcatt gtagtgatta catttatgac
    17881 aagttgcata tcaacaacac cacaaactac tcaaggaaaa ggaaataata aacttacaat
    17941 atatgcagca ctagaggaag agcagatagc ttattatatg gaaggtttta gagaagagta
    18001 tcccaatata gatgtagaga tagttatgga ttctcatggt gtaatagctt caaaactatt
    18061 agcagaaaaa gataatccac aagcagatat tatatgggga ttatcagcaa tgaacatgat
    18121 agagctaaaa aaagatggtg cacttgaagc atataagcct aaaaattatg ataaagttga
    18181 taaaaaattt aaagatactg aagatgatgt atcttgggta ggaatgagtg tcccagagac
    18241 agcaatagta gttaattata aggaacttaa gaaactaggt atagatattc caaaaagtta
    18301 tgaagattta ataaaaccag aatataaagg acttataaca atgccaaatc cagcatcatc
    18361 tggaacgggg tatcttacag tatcagggct tattcaaatg atgggtgaaa aagatgctta
    18421 caattacatg gataaattac ataaaaatat agctacatat acacactcag gttcaaaacc
    18481 tgcgaagtta gctggctctg gagagtatcc aataggtatt acacttggat atagagctac
    18541 acaacaatta tctagtggag aacctataga ggttgtattt ccaaaggaag gttcaggttg
    18601 ggacttagaa gcaaatgcct tagttaaaaa agataatata aaaaaagaag caaagctatt
    18661 tttagattgg gcaataagtg attcagctat ggatagatat gcacatgaag tagcagttac
    18721 ttccataaag acatataatc cagttccaaa aggttattct aaacaaccat ttgaacagct
    18781 tataaaagaa gttgatttaa actctgctgc caaaaacaga agtacagtac ttaaaacttg
    18841 ggaatcaaaa tacggttcaa aatctgaaag taagtaaggt ttaaattaat aatttagatg
    18901 aaagaatttt agaagaaaaa gtaggatata aaatttaaaa taaaaaaata actatataag
    18961 gagagataag atatgagtta cttaaaaata aataatgtgt ttaaaagtta tgaccaaaaa
    19021 agggttttaa ataatattag tttggatatt gaagaagggg aatttctatg tttactcgga
    19081 cctagtggat gtggtaaaac tacattactt agaataatag caggtcttga agatgtaaat
    19141 agtggaacta taattctcca ggataaagat attacaaatt tagagccatc aaagagaggt
    19201 tttggtatag tatttcaatc atatgcatta tttccgaata tgactgctta taacaatata
    19261 gcttttccac taaaagagag aaaagtttca aaagaaaaaa tagataataa agttaaggaa
    19321 gtacttgaaa cagttggatt gactaatgaa gctcataaat atcctaaagc attatcaggt
    19381 gggcaacaac agaggattgc aatagcaaga gcattagctc ttgaaccaaa gtttttactt
    19441 ttagatgaac caatgtccgc attagatgca aaggttagac acaagcttag aatggacatt
    19501 aaaagattac aaaaagaact taatataacg acaataatgg taacacatga ccaagaagaa
    19561 gctataacta tggcagataa aatagctatt ctaaatggag gagatataat gcaaataggg
    19621 actccagaag aaatatacca aaatcctcaa aacctattta cagctcaatt tataggagat
    19681 acaaactgtt ttgataatgg agattctata ttaactgtaa gaccagaata tgttcaaata
    19741 gagaaatcga caaaagaaaa ctaccaaggt ataatatcaa atatagagtt tagagggaat
    19801 ttattgagag ttgaaataaa agataaatta aatgaaaatt tcataataag tgatgtttct
    19861 ataaaagagt gggttaattt aaatttagta gaaggtgacc ttgtaaaaat aagtatagac
    19921 gaaaaatact atttaaagta tccaaaagtt aaaggagcat aagtgtatga ataataatgc
    19981 tataagttct aaaagtataa gtcttaaatc aaaaatgaat ttagatataa aaaaatgtat
    20041 gcttacagta ttagttatgt ctttagtaat atttttatta gcacctataa tatatctatt
    20101 tgtacaggct tttttagata aagatggagt ctttgtagga ttacaaaact ttcaagaata
    20161 cttggcttct gaatctctat taaattctct aaaaaataca gtatttgtat cagttatatc
    20221 aactgtaata tcactaacac ttgcattagt atatgcatat gcattaacta gaacaaagat
    20281 taaatttaaa ggttttttta aatcattagc tatgtttcct atgtttgctc caacaatgat
    20341 gcatgcaata ggtctagtat atttatttgg aaatcaaggt ttggtaacta atttacttgg
    20401 agtgcatgtt ccaatatatg gagcagtggg aataatattg gcacaaatta tatatgtatt
    20461 tcctcaatcc tttcaaatac tttatatatc tctaagttca accgattata gactatatga
    20521 agctagtaat actatgggga taagcaagat aagagaattt ataagcataa caatcccaag
    20581 tataaaatac tctttaataa gttctgcatt cgtatcattt acattatgtt ttacagattt
    20641 tggagctcca aaggtgatag gaggagagtt taatgtatta tcaatagatg tatataagca
    20701 aattataggg caacagaata tgccaatggg agcaactgta tcaatattac ttttaatacc
    20761 tgcaataata tcgtttatag taactaaaaa aattgaaaaa aatcaaaact catatataac
    20821 ttcaaagtca attccatata aggttaaaac taataagtta agagatagta ttttaggagg
    20881 ggttgtagga attattgcat ttggtataat agctgtaatg ttaactgtaa tcttatcctc
    20941 attaatatca gtttggccat atgatttaag acttacactg gaacactttt ctatgaaaaa
    21001 cagtattgat ggaattaaac catttataaa ttcaataaaa atgtcactat tgacagctat
    21061 tctagggacg agctttgttt tcttatttgc atatttaaat gaaaaaacag aaaaagctcc
    21121 atatttaaaa tcatttgggt attttttaag tacattgcct atggcattgc caggattagt
    21181 tttaggtata gcttatgtat tattttttaa taaattagag tttaatttta tggatacaat
    21241 atacattaaa aatttgttta atggattata tggaacatta cttataatgg ttatatgtaa
    21301 tatagttcat ttcttttcag tagcatttat aacagctact actgctatta agaaagtaga
    21361 taaagaattg gacatggtag ctaaatcaat gggaataaac agcgcatcaa ttcttaagaa
    21421 cgtaactata ccactttgtt tgccagcagt tttagagaac tttatgtact tttttctaaa
    21481 ctctatggtg acaatatcag cactagtttt tatatatact gcaagttcaa aagtagctgc
    21541 aatatcaatt gtacaactag atgataaggg gagtactgca caggcagcag cactagctgt
    21601 gttaatatta gtcacaaata tattagtgaa agtaatatat gaatttataa agaatcgttt
    21661 agaaaaaaga acaaacttta attaaataat ttatcttata aaaacacaaa tttaaaaagt
    21721 ctattcttat gatttattat ttaaagaata gactttttaa atttattatt gatattattt
    21781 attaattttc tttttgttat catacatttg agaatctgca cgacgatatg tatcaaataa
    21841 agaaacatct actttttcat caaaaatagc atatccaata gacattgata attttaattc
    21901 attagttgag ttttcttcaa ctatttttaa attaatatta tttataatac tatttatttc
    21961 tttgtgagat aaatttttta gaataattat gtattcatca cctccaatac gataaactat
    22021 atatttttta tttattacat cttgtataat tttacttgct tgcttaatat ataagtctcc
    22081 tgtctgatgg ccaaatttat cattaatctt ttttaaaccg tctaaatcta ttgatatgat
    22141 agatattaga ttctttagat ttgaattgtt taagttattc atatctattt caaaagcatt
    22201 tctatttttt aggcctgtta aataatctgt attagcaaaa tcagtatatg taggattaga
    22261 tattctttta aatagaatca ctgcaatcaa tgctgatatt agacaagtta ataatgctat
    22321 tattggtgta actatactta taaatttaaa tgtattgtat tgatgttttg cttcaaattc
    22381 aattcctatt actccaacaa ccttaccaac atcattgtga ataggaaaat acgatataaa
    22441 tatgtatccc caagatgttg atttaatctt tttaggtaac aatatatgat tatctaatgc
    22501 tttgttaaga tctccccaaa tctctttttc tatcaaatca ccagcatttc taaaatcact
    22561 tctagatgaa tttaaaccat caacaacata tactagtgat ccatcttttg tttttttagc
    22621 agtatataga tacatgactc cagtagtatt ttttacagat tctaatagga ttttagaact
    22681 tttataagac ttttttgatt ggtcttcttt agaattaatt tcagtaaagg tatttttatc
    22741 aatatttctc tctacatatt catagatatg ttttgacctc tcacttaaag agtatatcat
    22801 gctcttataa gttaatgtat aattaaaaaa atatacacat gaggaagata ttattacaat
    22861 aattgcagta agtattgaaa cctgcatatc aactctatta aattttttat ttattttcat
    22921 agcttaaatg tcttgtgaaa agaacctccc ataatctccc cataattaat atatatctat
    22981 aaatttcacc ataatatatt catatattat caaatttgtg tggaaattac aattagtaaa
    23041 catgcttaaa gtattcaaat atagtttttt tattattgca gtatactatt ttatgtataa
    23101 ataaaattat agataaatca cttatatttg ttatttctta atggaagata cattataaaa
    23161 tattctaatt gattacagta tgtatactta attgaagtta agttaagggt tataatatgt
    23221 attgatgaaa tgactatatt tatattcata agtattaatt ttatcagaga taggtaaaat
    23281 actttatctt aaacataagt atggttgaga tgcaatcttg agatatgata atataaattg
    23341 aaggatagtg aactcaatca aataaaatga ttaatctagc tatataagca aataaattta
    23401 atataaaaga ggtggaaaat gaaagacaga attaatttaa cagaaggaag aattacagaa
    23461 aaattactta agctatcact tccaataatg gggacttcat ttatacagat ggggtataac
    23521 atgatagata tgatatgggt tggtaaagcc ggaagtaaag cagtagctgc agttggaaca
    23581 gctggttttt ttccatggct tgcaatggca tttattatga tttcaaaagt aggtggagag
    23641 ataaaagtag cacaaagtat tggtgcaaat aatataagca ctactaaatc atatatgaag
    23701 tcagcaatag aaataaatat tatactagca ataatatata caatttcttt aatagtactt
    23761 aataaacaat tgataggttt ttttagatta ggagacttag aagttataac catgtctaga
    23821 cagtatttga taatagtagc tctaggtatg gtattttatt ttattaatcc agtatttact
    23881 gcaattttca acggtcttgg aaatagtaaa actccttttc gtattaatac agtaggatta
    23941 ataactaata ttatacttga tcctatatta atttttggat ggggatttgc tccaaaactt
    24001 ggtgttgcag gagctgctat tgcaactgta tttgctcaga tagtagttac tacatgtttt
    24061 atatatataa tagttaaaag taaagaagaa tattttaaat taagactatt taaagaaata
    24121 gagactaaat attataaagt tctgtataaa ttgggattac ctatagcaat acaaagtggt
    24181 atgtttacag tattctcaat gctattagga gtaatagtag catcatgggg gcctgtggct
    24241 gttgcagtac aaaaagtagg ctcacaaata gaagcaattt cctggatgac ggctgaaggg
    24301 ctatcagttg cattaggcag ttttgttgga caaaattatg gtgccaagaa gtattctaga
    24361 ataaataaag gttgcaaaat agctataact gtatcttctg tacttgggat aataactact
    24421 ttggtattag tatttgcagg agagagtata ttttctattt ttatagatga aagtgaggca
    24481 atagaaaaag gagcaattta tcttaaaata ttaggctatt ctcaattttt tatgtgttta
    24541 gaaataataa caacaggagc tttcaaagga ttaggcagaa cctatatacc ttctattata
    24601 attactatac tgactggtgc aagagtacca ttggcttact ttatatctag accagaaatg
    24661 ttgggtctaa atggtgtatg gtggagtata accttatcaa gtatattaaa aggtatatta
    24721 atgataagtc tatttatatc tttattaaaa ctcggaaagt tgtacaagat gtctgaataa
    24781 aatttaaatt atcttaaatg tatagacatt ttctttattt gtatataaaa ttatcttatt
    24841 atataaaaga tttaattaat aaggagtgaa ttaaatgaaa gagccaattt ataaagttat
    24901 agaaaatcat gttagagagt taataaattc agacagtttg aaagaagggg atttaatccc
    24961 ttctgaaaaa cagctgagtg aagaatttaa tgtaactaga atgactgtaa ggtctgcact
    25021 taataatcta gttaaagaag gctatataac tagacaaaga ggtgtaggta gtatagttat
    25081 tgcaaataat atatatgata atatatcgtc tgtaagtgga tttactaaag agatggaaag
    25141 taaaggctat aaagtatcta atatattggt atctcttgaa atagttcagg cagatgaaaa
    25201 tttatcaagt aagttgaata taagtttaga agaaaatgtt tgggaaataa aaagagtaag
    25261 attagccaat gatgcaagag tttcatatat gattacttat atgccagtta agttatttcc
    25321 aaatttaaat aagactcatt gtgaaaattc tctttataat tttgttgaag aagtgtgtgg
    25381 atacaagata gccatgtcag agagagaagt acaagctgta atatcgaatg aagaatgtat
    25441 ggataatcta aaattagaag aaccagagcc acttttatat ataagccaaa tttgtaagtt
    25501 acaaaatagt gagatttttg agtactcaca cacatatcac tatggatata cattaacttt
    25561 gaatgctgtt gtcgaataaa atttaaataa aatgaaaaag ttttcttgaa atgtatatac
    25621 atttggtata taata
//
LOCUS       DAEPOG010000039        24947 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEPOG010000039 DAEPOG010000000
VERSION     DAEPOG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.039"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            175..873
                     /gene="vanR"
                     /locus_tag="KR432_001356"
     CDS             175..873
                     /gene="vanR"
                     /locus_tag="KR432_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0639902.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            909..2024
                     /locus_tag="KR432_001357"
     CDS             909..2024
                     /locus_tag="KR432_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0639903.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            2169..3056
                     /locus_tag="KR432_001358"
     CDS             2169..3056
                     /locus_tag="KR432_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639904.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            3057..4292
                     /locus_tag="KR432_001359"
     CDS             3057..4292
                     /locus_tag="KR432_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0639905.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            4674..5591
                     /gene="pstC"
                     /locus_tag="KR432_001360"
     CDS             4674..5591
                     /gene="pstC"
                     /locus_tag="KR432_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH0639906.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            5593..6417
                     /gene="pstA"
                     /locus_tag="KR432_001361"
     CDS             5593..6417
                     /gene="pstA"
                     /locus_tag="KR432_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH0639907.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            6436..7200
                     /gene="pstB"
                     /locus_tag="KR432_001362"
     CDS             6436..7200
                     /gene="pstB"
                     /locus_tag="KR432_001362"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH0639908.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            7228..7881
                     /gene="phoU"
                     /locus_tag="KR432_001363"
     CDS             7228..7881
                     /gene="phoU"
                     /locus_tag="KR432_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH0639909.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            8039..8908
                     /locus_tag="KR432_001364"
     CDS             8039..8908
                     /locus_tag="KR432_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0639910.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            8995..10431
                     /locus_tag="KR432_001365"
     CDS             8995..10431
                     /locus_tag="KR432_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH0639911.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            10479..11306
                     /locus_tag="KR432_001366"
     CDS             10479..11306
                     /locus_tag="KR432_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0639912.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    11607..11869
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11945..14611
                     /locus_tag="KR432_001367"
     CDS             11945..14611
                     /locus_tag="KR432_001367"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH0639913.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     gene            15036..15746
                     /gene="rgaR"
                     /locus_tag="KR432_001368"
     CDS             15036..15746
                     /gene="rgaR"
                     /locus_tag="KR432_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBH0639914.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            16792..17823
                     /locus_tag="KR432_001369"
     CDS             16792..17823
                     /locus_tag="KR432_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0639915.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            17956..19254
                     /locus_tag="KR432_001370"
     CDS             17956..19254
                     /locus_tag="KR432_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH0639916.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(19368..19613)
                     /locus_tag="KR432_001371"
     CDS             complement(19368..19613)
                     /locus_tag="KR432_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0639917.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            19764..20630
                     /locus_tag="KR432_001372"
     CDS             19764..20630
                     /locus_tag="KR432_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH0639918.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(20634..21170)
                     /locus_tag="KR432_001373"
     CDS             complement(20634..21170)
                     /locus_tag="KR432_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBH0639919.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(21360..21563)
                     /locus_tag="KR432_001374"
     CDS             complement(21360..21563)
                     /locus_tag="KR432_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0639920.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            21854..22618
                     /locus_tag="KR432_001375"
     CDS             21854..22618
                     /locus_tag="KR432_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0639921.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            23007..24320
                     /gene="prdC"
                     /locus_tag="KR432_001376"
     CDS             23007..24320
                     /gene="prdC"
                     /locus_tag="KR432_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBH0639922.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            24489..24651
                     /locus_tag="KR432_001377"
                     /pseudo
     CDS             24489..24651
                     /locus_tag="KR432_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9886 a         2637 c         4339 g         8085 t
ORIGIN      
        1 ttgtatatct aaatctatat atattcattt ttatctttaa tatatgttaa ataataaaat
       61 agttacataa taagattaag aatttcttaa taaattctaa tgtaaaaaat catataaaaa
      121 aattaaattt agtataatat atataaatat atatagaaag gatgtttaaa gtctatgagt
      181 ggaaaaattt tagttgtaga tgacgaacgt gatatagctg atttaataga agtctacctt
      241 acaaatgaaa actatattgt ttataaattt tattctggtc ttgatgcact aaactgcatt
      301 aatttaaatg aatttgacct tgcaatttta gatgttatgt tgccaggagt aaatggattt
      361 tctttatgcc aaaaaatccg agaaaaatat acttatccaa tcattatgtt aacagctaag
      421 gatgaagaaa cagacaagat tacaggctta actttaggtg cagatgatta tgttactaaa
      481 cctttccgac ctttggaact tgttgctaga gtgaaggcac agattagaag atataagaaa
      541 tacagtggaa gtttacaatc tggtgaagaa ctttcacata ttttttcttt tttaggtctg
      601 gaattgaata cccaaacata tgaatgccat ttaaatggta aagctctttc acttactcca
      661 acagagtttt ctattctaca tgtattattg aaaaataaag gctctgtggt aaattcagaa
      721 aatctttttc atgctatctg gaaagatgaa tattacaata aaagtaacaa tactataaca
      781 gttcatattc gccatcttcg tgaaaaatta ggtgatactg tagatgaagc aaaatatata
      841 aaaacaattt ggggtgttgg ttacaagatt tgacacctat ttttcttgtc aagaagggat
      901 gtagtattat gaaaaagatt aataatttta cttataggaa actaagagat aaatataaat
      961 ttataaaaag agaaaaaaat acagaggcgt atttaaagta taaattatat ataatgttag
     1021 ctatctatac aggtatagta ttggttttaa tgtttatggc tgattatacg ttttcaaatt
     1081 tttcaaatcc tcttgttaaa tggcttaagt tgagaagaga tgtattattt tttttggttt
     1141 ttataattgg atatatcact attttcaatt tttattggaa aaaaccatgg aattatttaa
     1201 gagaaatcat caatgcaaca catactattt atcaacaaaa tgatgaattg gttgaactat
     1261 ctaagccatt aaaggaaata gagcaacaaa tgaaccaaat taagatgtct gttttgttaa
     1321 gtcaacaagc agtgaaagag gctgaaaata aaaagaataa ccttgtgatg tatctagcac
     1381 atgatataag gacaccactt acttcagtta ttgggtattt gagcttatta aaagaaatac
     1441 ctaatatgga taattctcaa aaatctaagt atactgaagt tgcacttgac aaagcacaat
     1501 atttagaaaa acttatcaat gaactttttg aaataacacg atataattct aatcaaatca
     1561 aaattaattt cgaaaaagta gacctatatt atatgttact tcaattaaag gatgaatttt
     1621 acccattgct gtctgaaaaa ggaaatagag ccattctaaa agcaaatgaa agtttaacaa
     1681 taaaagcaga ttctgaaaaa cttgcaagag tgtttaataa tatcttgaaa aatgctattg
     1741 cttacagcta tcctaataca gaaattatta tctcagcggt tcataaagac agcaaggtag
     1801 atattgcttt tagaaattat ggacagacaa ttccagaaga acaactttct gctatatttg
     1861 aaaagttcaa tcgtttagat gaagctagaa aatctgatat gggtggtgca ggtttagggc
     1921 tttctatagc taaggaaatt ataaatttgc atggtggtga tattactgtt caaagtaaag
     1981 atgaagtcac aacatttact atatcgcttc ctacatctat ttagtaaaat ataaatcaaa
     2041 tattaggatt ttcttaagaa attatctatt taatattaaa attatgagtg tctttgttta
     2101 gtaaaataaa tactaagcaa gacacttttg tttttgcagg taagtagata aagagaggag
     2161 tttcaaatat ggaattaaaa attgaacatg tgacaaaaaa atttaaggat aaaattgctg
     2221 tagaagatgt ttgccttacc ttaacaccag gtgtgtgggg atttctagga gcaaacggag
     2281 caggtaaaac taccttgatg agaattatta cagacattat gaaacctacc aatggcaaaa
     2341 ttctgtataa tggaaaaaat atttatataa tgggtgctga gtacagaaat ttactgggat
     2401 ttttaccaca agaatttggt ttcccacagg atttttctgt aaaagattat ttagaatata
     2461 tagcagcatt gaaagatgta ccaccaagag aagtaaaagg aaaaattaat aatctattgg
     2521 aaattcttac attatctgat gtaaaaaata aaaagattat taaattatct ggtggaatga
     2581 aacgacgagt gggaattgca caagccatgt taaatgaccc taaaatcctt ataatggatg
     2641 aaccaacagc agggcttgac cctggtgagc gagtgagatt tcgtaatttt atttcagaat
     2701 tttctcataa tcgtgttgtc ctaatatcta ctcacattgt atctgatatt gagtacatat
     2761 caactcaaaa tgctattatg aaaagtggaa aaattcttga tgtgggaaca accgataaac
     2821 tggttaagaa agtagagggc aaagtatggg aatgtcttat atcaaataga gatatttcaa
     2881 aatatgaaat gaatttacgt ataatcaatc agcgtagtga aaaaaacaat caagtatcta
     2941 ttcgttactt ggcagaaaag tcacaaattg ataactctat tgctgttgaa ccacgactag
     3001 aagatttata tttatggtta tttccagaag cagtttatag taaggagggg aaataaatgc
     3061 gtatttttta tttggaaatt aaacgagtgt tgcactcacg tcgtacatta attttattat
     3121 tgttggcatt ggttttatct gcattgatgg cttttcttcc aattacttac gagggtatca
     3181 attatttaga taataatggc aaggtagttg aattgagtgg tttagatgct attaaatata
     3241 aaaaaacaat ttatgctaat aatagtgaaa ttagtagtgg agttgtaaca ccaaaaaaat
     3301 taaagcatgc tttaaatata tggaaaaaaa cagaagaaaa gtatggtaaa gtaggtagtg
     3361 aagatttccc actagatatt tattctaaaa gtattttacc aatacgccca ttgttgaata
     3421 gacttcctga agcatttgct aattcagaaa caggatttgc aacagaatta tcagaaataa
     3481 atacaaatga tttagattct ttttatgaac aatgcaaaac tcatttaaat gatattatga
     3541 agattgaacg acaaacttcc agtgcacaaa tgaaagctca agaactttat agaaaagtag
     3601 atactccatt tgagatatac ccaggttatt ctaaagatgc tttcgattat ataatatttt
     3661 acatttttat attattaatt atatgtgttg caattactgc acctactttt tctggtgagt
     3721 atcagacaca atcagatagc atccttcgtt gtacgaaaca tggacatact cgtctagcgt
     3781 ttactaaaat attagctcta ttttctatat ttatagtaat gtttgttgta tgtatttcta
     3841 tccatttggc aatttctgat ttagcttttg gtactgaatg tttgaaaacc tcatttcaaa
     3901 tgttgttttc tgtcattagt ctagtaaata taaatttact acaagcacag attcttatag
     3961 ctattggtgg tctaatttca atacttgcaa tggttagttt aacattattt ttatctgcaa
     4021 aatgtaagga ttctcttact tctatgctga ttgctttttc tatttgctta attcctatgg
     4081 gaataggtgt tagttggatt ggatacctat taccaccagg gggaattggt cttaagatta
     4141 gtttattata tcaaatgaca tcatttaatt tcttacactt aggaggtatg agtttttgga
     4201 caccacacat tatactgttt ttctcagtga tagaaatacc tatttttata tttttggcta
     4261 ttcgtaccta ttgcaaacat caagtatcat aacaatttga tagaaataat agtgatataa
     4321 actagaggat acgataatta tatatgtagt ataaaagtta aatttagtta acaaatctta
     4381 atagcagata taggtaataa attatacata aactgtatta ttatctatat ctgctatttt
     4441 aatatagaga aacaacagta tattgtaaag taaatgtcaa atatagtttt aaaagtaaaa
     4501 ttaatgttaa aaaagtaaat ataattttac acttgcttaa cttaatccta atatagattt
     4561 aacttaaaaa aagtagaata aaagtacagg aaatttgatg aagacgaatt ataaaaaatt
     4621 aaattttgaa tacagaacga aaatcgataa gtatatttta atgggagggt attatgatag
     4681 taaaatctag attagtaggt ttagaaaaaa atgacaataa tggcaataag acaaaatacg
     4741 taattgaaaa agtagcaaaa aatatttttc tacttagtgc tttagtagca gttgcaagtt
     4801 tacttttgat aataggattc gtattttata aaggcttaag accatttata tttgaaggat
     4861 attcatttat agacttttta actggtgcag attgggttcc ttctgccaat aaatttggaa
     4921 tatcatctat gatagtagca tcaatatttg caacacttgg agcacttgtg ataggagtac
     4981 ctgttggtat attaacctct gtatttatag cagaagtagc acctaaaaga ttagcaaaaa
     5041 taatgtcacc agcagttgaa cttttagcag gtataccttc agtattatat ggtgtatttg
     5101 gtcttgcagt gatagttcca actatacaaa gtatatttaa tcttccaaaa ggtcaaagtt
     5161 tgttagcaat agtaatagta ttagcagtaa tgatgttacc aacaataata tctgtatcag
     5221 aaacagcaat aagagcagta ccaaaagcat acaaagaagg ttcattagca cttggtgcat
     5281 caaaaataga aacaatattc aaagttgtac tacctgctgc gaaatctgga gtattagctg
     5341 cagtagtatt aggagtagga agagctttag gtgaaacaat ggcagtaata cttgttgcag
     5401 gaaactcacc agttatgcca actgcaataa cagatagtgt aagaccactt acaactaata
     5461 tagcacttga aatgggatac gcatttggta cacatcaaga aatgttattt gcaactggag
     5521 tagtattgtt tacatttata ttaattttaa acttagtttt aaataaactt tcaaataagg
     5581 cggtaaatta agatgagaaa gttcaaagag aatctattga agtcattagt atatctatca
     5641 gcactattta caatagtatc attagtcatt atagtgggat ttatatttat aaagggaatt
     5701 ggcaacatga attttaattt cttatttagc aattattcag catcaggtga tggtggtata
     5761 cttccaatga taattacaac attatataca gtagtagttt ctatagttgt agcaactcct
     5821 ataggaatat tggctgctat atatttacaa gaatatgcta aaaaaggaaa agcagttaca
     5881 gctataagat ttgcaactga aagtttatca gggataccat ctatagtata tggattattt
     5941 ggaggaattt tctttgtagt tacattaaaa atgggatatt ccatagttgc aggttcactt
     6001 acagttgcaa taataatatt accagtaata attcgtacaa cagaagaagc tttaaaaaca
     6061 gttcctcaag cttatagaga atcatcattg gcattaggag ctacaaaatt tcaaacttta
     6121 tataaggtag tacttccaag tgcaatacca ggaatcttat ctggagtaat cttatcagtt
     6181 ggtcgtataa ttggagaatc agctgcaata ttacttacag caggtactgt agccaagatg
     6241 ccaggtggaa tatttgatag tgcaagaaca ttaactgtac actcatactt actgactaaa
     6301 gagagtggag atatagcaac agcagccagt ataggaattg ttttaatagt aattgtatta
     6361 gcactaaaca tgctagcaag atttgttgct aagaaattaa ataaagctaa ttattaaatt
     6421 taagaggtgt gaataatgga attaatcgat aagataaaaa tgagtgtaaa agatttggat
     6481 ttattttatg gtgataagca agcattaaag aaaataaata tggatataaa agagaataag
     6541 gttactgctt taataggtcc atctggatgc ggaaaatcaa cttttataag aacgttaaat
     6601 agaatgaatg acttaatcga agatgtaaca attaaaggaa acatttctgt tgatggagag
     6661 gacatatata cctcagatga tgttataaat cttcgtacta aagtaggaat ggtatttcaa
     6721 aaaccaaatc catttcctat gagtatatat gataatgtgg catatggtcc tcgtacacat
     6781 ggactaagag ataagaagca attagataaa attgtagaag aaagcttaaa aggtgcagct
     6841 atttgggatg aggttaaaga caggcttaaa tcatctgcac taggactttc tggtggtcaa
     6901 caacagcgta tatgtatagc aagagcaatt gctatgagac cagaagttat tcttatggat
     6961 gaacctacat ctgctttaga cccaatttca acattaaaag tagaggaact tatagaggat
     7021 ttaaaaaaag actacacaat agtcatagtt acacataata tgcaacaagc tgctcgtata
     7081 tcagatgaaa cagctttttt cttaaatgga gaggttatag aatttagcga tacaaaaact
     7141 atgtttacca ctcctgtaga taaaagaaca gaagactata taacaggtag atttggttaa
     7201 aatatataac tataaaggag ttagacaatg gtcaataata gtttagatat aagtattgat
     7261 actttaaaag aacaaacagt acttatgatg gaaaaatgtg aagaagcaat agaaaagtct
     7321 gtgacttgta tgatagagaa ggatttagag ggtgcaagaa aagtagtaga agatgatgaa
     7381 gaaataaata aattaagaga agaaataaga gataagagta tagagctaat agtattaaag
     7441 caaccaatgg caagggactt aagatttatt tattcattag gtaatatatc gatagagttg
     7501 gaaagaatag gagattatgc agcaaatata gctagagaaa tgttgaagat aggtggagag
     7561 gcttatataa aagagctgat agatattcct aagatggcaa aagaatgtaa gtctatgatg
     7621 tctcagacta gagaagcttt aatcttattt gatgcaagta tagcttatga tgttggaact
     7681 aaagatgata ttgtagatgg attgtatgaa caagtacaaa ttgattgttt aaaaataatg
     7741 catgaaaatt caaatactat agaccaagga gtaaaacttc tacttatagg aagatacctt
     7801 gaaagaattg gagaccatat aacaaatgta tgtgagaaga taatttttgc aattgagggt
     7861 aaaatggttg agataggata aaaaactgct ttaaagcagt ttttttattg tctaaattaa
     7921 gaaagaattg ataaaaaaat atgattaatg ggtaaattta aaaaattgtg atatactagg
     7981 aacatattaa atatgaggat gcgtatttta tataaggtgc tttttaggag gtggaacttt
     8041 gaaacttcaa aagatgggta tgaggactat aaaaacagga attagtgtca ctttatgtat
     8101 gattcttggt aactatctag tagagaatat gttttattct ctagtagcat gtataatttc
     8161 tgttcaagat acggtaaaag gttctttaaa gacaggaatc aatagagtgg aaggtactat
     8221 tttaggtggt ataataggat tcatatttgc catcattaaa gctggagacc cttttttatg
     8281 tggtctagga gtaatgtgct caatatattt atgcaatctg tttaaaataa agtcagtagt
     8341 agttgcctgt gtaacctttc tggctataca tctaggtgta ggaaatagtg accctgtgta
     8401 ttattcttta catagagtgt tagatacatc tgtaggtgtt ttgtttggag tacttgttaa
     8461 ttattatgtg gctagaccaa attatctaaa tagtgttata gatgaattta aaaagataga
     8521 agaactttca atgttactta ttgaagataa aattcttaga aaagaaaatt taaatgtaga
     8581 gaaatttgaa gaagaagtag aaaaattaga agaaatatat tcaaaactta ttgatgagtt
     8641 ggattatagt ttgagtgaaa ctaatataga aaaaattgag aatgccttaa ggatatgtag
     8701 agaaatacat tttcatatgc agtctataga gttacttgaa aataaacttt atttgaaaga
     8761 gcatacatat aaatacctta agaaaacata taatgtggat aagctagact ggaatataga
     8821 tgaagaaaaa agccccgtat tcaactatca tctaatgaaa ataatgattg aaatagagtt
     8881 gttgagaaag ctaaataaat catattaata atagtagtta aataataaat aaaaaatagt
     8941 atctttgtaa atattaaaca tatataagtt taatttatgg aggtactatt ttttatgaat
     9001 actagaaatg acaattctaa aatactagaa atatataaaa tgaagctttt tggcgaactt
     9061 gtaaaagcag tgtggaatga tgatatagag gatattgaaa acttacctaa taaaatttta
     9121 aatgaagatt taaaggataa tttaaataat tattttagtg gaaaatttga taaagatact
     9181 atggtaaatt taattagggt aactatggga ttggatgtga cagaagatac agataaaaat
     9241 ttaaaagagt cactacatga agctgtaaat ttagatgaag tcaaaagacc aataatatct
     9301 gtaatatcag atgcttgcaa atattgtgat gaaaataata aggaagatga gtcatgtaag
     9361 ataagaagta agcatattaa ttgtaatgag gaagatgtat gtagtgcttg tggtgattgt
     9421 gtatcaagat gtaagttggg tgcaatttca gataaaatag aatttatacc aatgataaaa
     9481 ttgttaaaag atataaagag ccctgtatat gcaatagttg caccagcttt tgtaggacag
     9541 tttgggaaag atgtaagccc tggtaaatta agaactgcat taaaaaagat aggatttgaa
     9601 gatatgatag aagttgcttt ggcagcagat atgcttactg caaaagaagc atatgattat
     9661 tatgagcata tgaaaaagga cgaagaagga tgctttataa ctagttgttg ctgtcctatt
     9721 tgggttagtt tgatacaaaa tagttttcct cagatattgg agaatgtatc accatccgtt
     9781 tctcctatga ttgcttgtgg gagagctgta aaaattttaa atccagatgc taaagttgta
     9841 ttcataggtc cttgtatggc aaaaaagaaa gaagcaatgt tagaagatat aaaagatgca
     9901 gttgattttg tgcttacatt taaagagttg caagaaatat ttgatgcatt agagttaaat
     9961 ccagcaataa tggatgagag taatagagta gaggcgtccc tttcaggtag agtttatgct
    10021 agaacaggag gtgtaagtaa agcagttaag ttatcagtca gaagaattga caaaaatata
    10081 aaatttgaag aaaaggcatt tgaaggaaca aaagagtgca tggacggtct aaagaaagtt
    10141 ctaaataaag aaatagaagc tacatttata gaaggaatgg ggtgtgtagg aggctgtgta
    10201 ggaggtccga agcgtatttt aagtgtggaa gaaggtacta agaatgttga tgaatatagt
    10261 gaaagtacga atatggaaac tccatttgat aatttaaatg tattacaatt tttaacttct
    10321 ttgggaatca aaagagtaga atctttagga gaaaaagaag aagaaaaggt taaaaaaata
    10381 tttagtagag atataaaaag taataagaaa gagaataaat ataaaaaata actttttaag
    10441 tcttaaactt atgcttttat aaaagggaga tatgggggat gaaaattaaa gataataagg
    10501 ctgtttttca tgtatttata aggatatttc ttatagctgt tttaatttta actggaacat
    10561 tattggagga aaaaattaat aatgttccag ttgaaaatat aagttcaaaa ggtgaaaata
    10621 cactggaact gctttctcaa agtaatactt taggagaaat catatacaaa gcatctcaag
    10681 aagaaacaaa aggtccaggg gctacttctg aaaaagtagc atacataaca atagatgatg
    10741 gtccatcaaa atatacaaat gcactacttg atattttaaa agaaaatgat gtcaaagcga
    10801 cattttttat gcttaatcaa aatatgaaaa atcacaagga agaagtaagg agagtcttgg
    10861 aagaggaaaa ctcaataggt tttcatagtg taagtcatga tatacataag ttatataaaa
    10921 gcccagaagt aacagttggg gaatttaata catgcaataa tactctacaa gaaataactg
    10981 gtcagagttc aaaattaata agattaccat acggaagtaa accatatatg ccagaaggtt
    11041 catataataa acttatggca aatgattatt taatttggga ctggaatttg gatacacagg
    11101 attggaagtc ttctacaagt cagatagtaa gtaatatatt atattatgga agaaaaagag
    11161 atgaattagt agtattaatt catgagaaag agcaaagttt aaatgcttta aataatatta
    11221 taagaatctt aaaagagaga ggatatacaa tattgccaat tacagaaaat ataacaccta
    11281 aaaatttctg gcaagaaaac ttataataaa tttaaatata gtaaaagata aagtaaatta
    11341 tttataaaat aaaacatata atataaaaaa tacgaataag aggtatactg taaggataat
    11401 ataatataaa attaatgaga aagcattaaa attaaaataa aataatcttg caattattga
    11461 cattataaga cagaataact gtaaaaacat attttaaaat ataacaacta tataaaagag
    11521 aatagacata attaagaaaa ttcagaaaaa ataaaataaa aataattgac aaagagaata
    11581 atatttgtat ataataatat gtaatattaa agactgagaa gaagaggagt aagtaaatac
    11641 tactaaaaga gaggaaaatt cactggctgt aagattttct aagtataaga tttactgaat
    11701 gtagctttgg agtttctaaa ctgaactata agtaagttta gacggttaga accgttaaat
    11761 tctttgagag tcaaagttga taaaataaca ttaactaata atttaattat atgttaatgt
    11821 attttaagct ttggaattaa ggtggtaacg cgagcttttc gtccttttta aagaggatga
    11881 agagctcttt tttatttcta aagatattta aaaaatttat ttataaaata caggaggcag
    11941 gacaatggaa aatacgaatt tatcaaaaac ttataaccca aaagattttg aggctaggct
    12001 atataaaaaa tggatggatg aagggtattt taaatctaaa ccaaatccag ataaaaaacc
    12061 atttacaata atgatgccac caccaaatat aacagggcaa ttgcatatgg gacacgctct
    12121 tgaccataca cttcaagata tattaatcag atggaaacgt atggatggat atgaagcttt
    12181 ttggcttcca ggaactgacc atgcttctat agcaacagaa gttaaagttg ttgaaagaat
    12241 aaaaaaacaa gaaggcaaga caaagtatga aataggtaga gaagaatttt taaagagagc
    12301 ctgggaatgg aaagatgaat ttggaggaaa aatatctaat cagcttaaac aactaggtga
    12361 ttcttgtgat tgggataaag aaagatttac tatggatgaa gggtgtaatg aagcagttat
    12421 agaatttttt gtaagcctat atgaaaaagg tcacatatac agaggaaata gaataataaa
    12481 ctggtgccca gattgcaaaa ctactttatc agatgcagaa gttgaacatg aagaacacga
    12541 cggaaacttt tatcatataa agtacccact aaaagatagt gaagactttt tagaaatagc
    12601 tacaactaga cctgagacaa tgataggaga tactggtata gcagtaaatc cagaagatga
    12661 tagatataaa catttaatag gaaaaacagc aatacttcca ttagtaggaa gagaacttcc
    12721 aatagttgca gatagttatg ttgatttaga gtttggaact ggtgcagtta agatgactcc
    12781 agcacatgac cctaatgatt ttgaagtagg attaagacat aatcttgagc agttaaatac
    12841 tatgaatgaa gatggaacta tgaatgaagt ttgtggaaaa tatgaaggta tggatagatt
    12901 tgagtgtaga aaagctatcg tagcagactt aaaagaacaa ggttacctaa ttaagataaa
    12961 agcgcataat cataatgtag gaacttgtta tagatgccat acagtagttg agcctagatt
    13021 atcagagcaa tggtttgtta agatggaaga acttgcaaaa ccagctatag atattcttaa
    13081 aaaaggagaa ttagaatttg ttccagataa atttgataaa acttatttac aatggctaga
    13141 aaatataaga gattggtgta tatcaagaca attatggtgg ggtcatcaaa taccagctta
    13201 ttattgtcaa gagtgtggag aaatagtagt tgctagaaaa atgccagata agtgtcctaa
    13261 atgtggaagc actcatttta aacaagatga agatgcctta gatacttggt tcagttctgc
    13321 attatggcct ttctctactc taggatggcc aaataagact gaagctttag attattatta
    13381 tccaacaagt gtacttgtta caggatacga tataatattc ttctgggtag ttagaatggc
    13441 atttgcaggt atgttctgta tgaatgaaaa gccatttgac catgttttgg ttcatggatt
    13501 agtaagagat tctcaaggta gaaaaatgag taaatcttta ggaaatggta tagacccatt
    13561 ggagataatc gagcaatatg gagctgatgc cctaagattt acattaacaa ctggaaattc
    13621 cccaggaaat gatatgagat tctatatgga aagagtagaa tttgctagaa actttgcaaa
    13681 taagttgtgg aatgcatcaa gatttgtatt tatgaatatt gatgaagata taattaaaaa
    13741 catgactaga gaaagtgtta aagaggactt aactctagct gataaatgga taatatcaag
    13801 agcaaataat attgtaaaag aagcaactaa taacatggat aagtttgatt taggaatagc
    13861 acttcaaaag atatacgatt ttacttggtc agaatactgt gactggtata ttgaaatggt
    13921 taaacctaga ttatatgggg aagatgcaaa tgctaaatct gcagcattat atacattaac
    13981 ttatgttctt gaaaaaatat taaaattatt acatccatac atgccattta taacagagga
    14041 aatatatact catcttccta ctgttgaagg atgtataata gtatctgaat ggccaaaata
    14101 taatgaagaa gataacatgg ctgaagaaga agatatgatg aacttattga tggaaggtat
    14161 aagaagtata agaaatgtga gagcagaaat gaatgtacca ccttctaaga aagctaaatt
    14221 gataataatc ccaagtgaag aaaaaataga agctattgag cttggtaaag actattttat
    14281 aactctagca tctgcttcaa atgtagagat agcaaaagat aaatctaatg ttccagaaga
    14341 tgcagttgga gtagttattg atggtgttga aatatttata cctcttaatg aattagttga
    14401 ttttgaaaaa gaaattgaga gattgtctaa agaaaagaaa aagttagaag gcgaaataaa
    14461 gagagttaat ggaaagcttg caaatcaagg tttcttagca aaagctcctg aaagtttaat
    14521 tgaagaagaa aaagtaaaaa aagaaaaatt tgaagagatg attaaatctg ttgaggaaag
    14581 attaacaaat ttagaatcta aaataaaata aatataagaa ttagtaaaaa catacaaaaa
    14641 gtatatattc tatacttttt gtatgttttt ctttatatat atacaaatat gatgttataa
    14701 ttctttacag atgtgttata tttttatttt atgttgtgaa tatatgaatt ttatgtctta
    14761 aaatttataa gcaataaaaa tttgatatat tatgatgttt aatatagggt aataaataca
    14821 tattatattt attttactat atttttttac ataatatatc tagaaaagta taggaattta
    14881 tcgtaattta agtttaaata ttaaagagaa tttctataag tagtttaatc gacaacttaa
    14941 atttataaag aaatttaaat ttgaaagcta gtgagtacag tttggaaatt gaaataaagt
    15001 aaactaaatg atattttaga tttggaggtg atattatgtt cagggtagtt atatgtgatg
    15061 acgaaaaaat acaaagaagc atactaaaag agtttacaaa aaaacttttg tctgaaagat
    15121 gtctaaatta tcaaatactg gaattttcct gtggagaaga tttaatatcc aaatacccag
    15181 aaaaaattga tattatattt ttagatatcc aaatgaaaga tatcaatgga attgaggtgg
    15241 ctaaaaaaat aagaaagttt gatgaaaaag tagaaattat atttacaaca gctttttcag
    15301 agtatgcccc ccaaggttat gaggttcgtg cttatagata tttagtaaag cctatagagt
    15361 atagtaactt tgcaatgggt gtaaatcttt gtattgataa ccttcaaaga aagaaggagc
    15421 gttatatagt tttaaatagt aaaaaaggat ttaatagaat actgatagat tctattttat
    15481 atgttgaaac tgtaaagaga gatttagttg tacatacaat aggtagagat tataaagcta
    15541 atatgagcat gaaacaagca gaaaaattac tgtgtgaaaa tggctttttt agatgtcata
    15601 caagttttat tgttaattta agaagaatag aagatcttag ggataatatg attactatta
    15661 atgaacaatt tatacctata agtaagtata gacttaaaga tttaaaagta gcattaacaa
    15721 gcttgttatg tgatataatg tattaatttt aaaaaggtaa ttgaaaatat cgtaaaaaag
    15781 atttaatgtc atcacagggc tattgattaa aactgtctta atatataaaa tgttatttat
    15841 attaagacag ttttattatg caaaatatca gtttatagac ttttaatatt gtttaatatg
    15901 tatatgtaga gtttccatta cagtagcatg attattaatt agtagcctga ttattaatat
    15961 actattattg atttttatat gcattatatg tatttttaga atatgattac ctttttaaaa
    16021 taatgactat ttataaaact ctatagatat ttgtttcatt tagctatgca tactttaatt
    16081 aattatatta atatttaatg gagtataata taaatattaa agcgacatct tatagtttat
    16141 tgaacctata gtaatgaatt aataaatggt ttaattctat gaaaagattt ccttttatgc
    16201 aatttattta ttgaaatatg atataatata taaaggtgta agtattagtt gttaaaataa
    16261 tatttatatt agtttttatg catacttggt ataagttttt catatattgg ttaaaattga
    16321 tgttgattta ttatttttag tattttttca ccttttgatt catactttgt agaatggtgt
    16381 attttataaa actgattata agacaagttc attcaaatta gcatttttat atcatataaa
    16441 taattgttcc ccctaaataa gatttagatg tatagtatta aaatcaaaaa gtataaaata
    16501 aaattgatgc ttaatcatag ataaatatta ataaaatata caaggggatt gtctaataaa
    16561 aagataatct ccttttttac aaataatcct aaattgtgat ataaatctaa aaattttatc
    16621 ttaacattta accatttaat gataataatt gatataataa atataaatta aatatatggt
    16681 gtacttattt tatgttttta gcaagaaata tagcatttaa aagataaatt gtgcaactta
    16741 aatcaaagtt taagatgaac tattaacata gatatattaa aaacttttgt aatggagaat
    16801 atatgtgata tgaataataa agttaaggtt gcagtattag atacaggaat agacaaagaa
    16861 catgattact taaaagataa tttagttgga ggaattgctt ttgaatgtat gcatgattat
    16921 atctttatat cagataaatt tgatgatgaa gatggtcatg gaactgcttg tgcatcaata
    16981 ataaagaaag agtatgaaga tgtagagtta tttgtaataa aaattttgag aaaagatggt
    17041 attacaaata taaaagttct tgaagaagca ttaagatacc ttttagacac taacataaga
    17101 ttaattaatt taagtttatc agtcatagga gtagagagtg ttaagggctt atttgaagtt
    17161 tgttacgaat tgtttagaaa aggaaaaata atagtatgtt cactcgctaa tgattttgat
    17221 ttaagttatc cagctatgtt taacaatgtt ataggtgtaa gaggatttac tttagatgga
    17281 gatgattcat tttggtacaa caaaaaatat gatgttcaat gtgttatgga tagcaattca
    17341 tatataagtt gtgatataaa taattcctat agattaccac caaaatgtaa tagttattta
    17401 tcagcaaagc ttactggtaa aatagctaag atattatcag aaaaaccagg cataacaata
    17461 tctgatttaa atgataagct tgaatcatta gcaactagaa atcattggga tagttgtgat
    17521 tttgatgagt gtagtagaat tgtaaacttt aaattggatt tgtatgacaa agaaaatgct
    17581 cttcttatga aagttgctga cgttgttagg gattgtttag atattgaaga taataatgaa
    17641 aaactcttta agggtagcct ttttaataag gagataggtt tggtttatga taattgtttt
    17701 aatctgttga aaaagctaga gcatagattt gacataaagt ttaattatat ggacatctca
    17761 aaatatgact tagtttcaat atatacactg actgaattag tggaaagata taaaaactta
    17821 taaataaaga tttgtagcta aatttaatgt agaaggtatt aaattatatg tattctttgt
    17881 cggtagatta aaagtaaaaa atatactata attagatagc ataagagaaa atgccaatac
    17941 agggggagtt agataatgaa atatgaagaa gcattagagt acatatcaca aactaataaa
    18001 ttcggtataa gattaggatt agaaaatatc ggaaagttat tagaattatt aggaaatcca
    18061 caagaaactt tgaatataat acatgtagct ggaactaatg gaaaaggttc tgtgtgttct
    18121 tttgtttcta atatactaag agagtgtgga tataaagttg gtttatatac atctccatat
    18181 ttagaaactt ttactgagcg tataagagta aatggacaaa atataccaca ggatgatgtt
    18241 gctagaataa ttgagttaat aaaagaaaaa atagagatta tggtaaaaga aggctatgca
    18301 tatccaacag aatttgaagt ggttactgca atggcatttt attactattc agaacaaaag
    18361 gtagactttg tggcattaga agttggttta ggtggcagat atgatgctac aaatataata
    18421 acaaaatcac ttgtaagtgt gatagtatct ataagtttgg accatacagg tatacttgga
    18481 gatacaatag aaaaaatagc gtacgaaaag gctggaataa taaaggaaaa tggagtagta
    18541 ctagtatatg accagactga tgaagcgaag gatgttataa agtcagtttg taaagagaaa
    18601 aaagctaaat atatagaagt tgattttgat gatataaaca taaaaaaatc agatataaat
    18661 tctcaaatat acgattgtac tgtaatgaaa gaaacttata gagaccttga aataaaattg
    18721 ataggtgaac atcaaataaa caattctatc ttggcaatta gtgtaataaa atatctaaaa
    18781 gacataaata aattagcaaa tataaatgaa gaatctataa gaaaaggact tataaatact
    18841 aaatggccag gaagaataga aaagataaaa gaaaatccaa tttttataat agatggtgca
    18901 cataatgaag atggagcaaa atctttggca aaagcacttg ataagaattt taaaggtaga
    18961 aaattaacct tattaatagg tatgttagaa gataaagata ttgatagtgt tttagagata
    19021 ttgttgccac attttaataa ggtaataaca acaacaccaa ataacccaag ggccataaat
    19081 tcagatatat taagagaaaa agtattaaaa tatgtagatg atgtaacatc aaagcatgag
    19141 atagaggatg cagtaaatta tacattagaa acatcaagtg aggatgatat tataataagt
    19201 gcaggttcat tatatatgat tggtacagtg agaactttag taaagaaatt ataattttat
    19261 aaaataaata tacaaataaa gatataaaaa agagttgtct aaaaaatgga caactctttt
    19321 tatataaagt aagtttttaa taattttact gatgtttata taagtaatta tatagattga
    19381 cgtatagcct tagataattg gtctgggcaa gaagtacctt ttccaccaca gtcaataccg
    19441 tttaatttat cagcaacttc aagagcattt tggccgatta ttaagcttct aagtcctgat
    19501 aaattaccat tacatcctcc tataaactca gcatcaacaa tcacattatt ttcatcaact
    19561 tcaaaaatca tttctctaca acatacacca cttggattaa atgttacttt cattaaaaag
    19621 acctccatta aatattattt ctttttttaa caataataat aataatacca aatatatgcg
    19681 taataatgca actataaagc ataatacaag ctaatgaaat aatatattat tgtagtatat
    19741 gtattgtaga gaggaggggt tatatgaaaa tctatatttc taactactta tcaaagagta
    19801 ttagcatagt tgattattct acacttgaac tagaaaaaga aatagtatta gaagataata
    19861 tatatccaca ccatttttgc atagaaaaag aaaaaaattt aatatacata cctagttcaa
    19921 gtaatgggat actttatgtc ttagatttaa gtaatgataa aataattgat actgtctcta
    19981 taggaggaag tcttagtcaa gttgtactta gtgataatga attgtttgtg gcaaatgaag
    20041 attctaatag tatatatgta ctagataaaa acacattaaa tccaatagga attattggag
    20101 ttgataatat gccacatgga tttgactttg atagagaaag taaaaaatta tatgtaccgt
    20161 gtataaactc gatagtatgt atagatacaa ttaataaaaa tatacaaaag aaaataaata
    20221 tggactttag agcttggcat atagagcttg ataaacaaaa gaaagaaatg tatatatcaa
    20281 cacttgatgg aaagcttata ataatagatg aagtaagcat ggatataaaa aaggtattat
    20341 atgaattttt attaccagta gaaataagat ttaattatag tggcaaaaaa gtttatgttg
    20401 ctgatttggg ttataataat gtaagaattt tagattatac aacgagtaga tacattggaa
    20461 atattgaaat aaatgggatt cctcaaggct tagaaatatc taaagatgaa aagctactat
    20521 ttgtatcaga tacacaagaa aattcagtaa aagtttatga aactgcaaac aataaattaa
    20581 taaaagaaat caaggtagga aaagagccta caactataat ttgtttgtag gctttaatgt
    20641 atgtctctta taagtaaatc gataacttcg gcatacatat aagatgcatt atttttccag
    20701 ttattgcaaa taagttttgc ttgtttctct gatgaaacat ttagatttaa atctattaag
    20761 ttagactgat tttctatgac tctaaggttg acaataaatt cattatcact ttgttttaca
    20821 aaactagact ttacttgagt atctgctagt agattttctt tgttagaatc tatatacttg
    20881 tctatttttt cagtaacact agtaggtatt cgattaaaga agtattctag actttctacg
    20941 cctctttgag taagagcata atattctctg tcagagtctt tatatgtagt caaaaactta
    21001 gattccataa gctgagataa aagctgttgt aatgaaaaat aattcatcat ctcagtctct
    21061 aatactactt gagttatttg agaattagtt aaatccattt taattttttc taatatatat
    21121 aaaattaaaa gtttgtgata ggctaattct tcagacgagt tttcaaacat aaatatcatc
    21181 ctccgtcaaa ctatttctat atataatata ttaaactaat ttgtacaaaa ttaaaagaac
    21241 aatttgtatt gtattattaa tctttaataa ttcatataaa acaattttat aaataaatat
    21301 agagaaacag ggctgctaat tagcagccct gttgataccc aggtgtcaaa atttactatt
    21361 tattttccag ccatttgtct ttcagccatt tcaactaatt tcttagtcat atatccacca
    21421 acatatccat tttctctagc tgttaagtta cctttatcta tgttttcata gttagataaa
    21481 cctatttcgt tagctatttc taatttcatt tggtttaaag cagcttttgc ttctggaact
    21541 actgttctgt ttgaatttga catagtaaat ttcctcctca aataattagt ttatgatgtc
    21601 taacaacact tttatttatg tgatgttaat actaatttct ccaaaataaa aataatatat
    21661 acagaaaaat aaaggtaaaa atgtaaaaaa tataaaaaaa ttaaataaat gagtatttta
    21721 gagagtttaa ttaggtcaat ataggtttta tttttttagt tattgtctta taaattaaat
    21781 attacagtac ttttttgagc tgaaaaaaaa taaattttaa atatagaaga aattatttca
    21841 aagggggaat agtatgaaaa gagaagttaa aaggactaaa gctttgatat atagtgtttt
    21901 atttttttca ttattattag tgttaatatt tgtttttgtt tatggaataa aatacatatg
    21961 ttctaaatac gacaattcaa aaatgcctat atataaagtt gatacaaatg agaaaaaaat
    22021 agccttgagt tttgatgtag catggggaac tgataatatg gatgatattt taaaaatttt
    22081 agataaacat aatgtaaaag ctaccttctt tttagttggt agttgggtgg atgataatga
    22141 agaaattgta aaagctatag atgaaaaagg acatgaaatt ggaaatcatt caaacactca
    22201 tgccaatttg aaggaaatat caaaagaaga cataacaaaa gagatagaaa ctacatcaga
    22261 aaaaatatat aatataacag gaaaaaagac taatttattt agacctcctt ttggggacgt
    22321 aaacaataaa gctatggaca tttgtagtga tttaggttat aaagtaatta agtgggatgt
    22381 agattctata gattggaagg agcttggacc aaatcatgta attgagaaag taataaaaga
    22441 atctcaacct ggttcaatag ttttattcca tgctaatata aatgatgtag ataactattt
    22501 ggatactata ataagtcgac taaaaaaaga tggatacagt ctagtcaaaa tatctgattt
    22561 gttgtataaa gataattatg tagttgattc caatggagtg caaaaaacta agaattaata
    22621 agtaataaat gtatatttta agtaaagttg tgataaatta tatagtatat gttagaaaag
    22681 tgtcgataaa aagacggtgt ctattttttg acacttttta tacatgtatg caatatcaat
    22741 aattatagat taaattgatt aaaaaagtgt ctaataattg actttttttt gatggattta
    22801 aaaatttaga agaattaatg aatgctgtaa gacagtattt ccaaagggta taagtttgtt
    22861 taaaagttgg catagaaatt gctttgttaa taagcgatat gttctataga aataaacaat
    22921 atgttaagac cttataatag aaaaatagca ttaacattat ttgaacagct tataaaaaaa
    22981 ttaatatact taagggggcg tactagttga acaaactata ttctatttta ttaaaacaac
    23041 acgttggtgg tccagataag ccagtagtaa gtgttggaga tgtagtaaaa aaaggaactc
    23101 taatcgcaga accaactgga ttaggggcaa atatttatgc cagtgttagt ggaaaaataa
    23161 gtgaaataaa tgaccaagcg atagtaattg aagctgatga agtacaagaa gatacttttg
    23221 aaccattaaa aggagaaggt atacttgact taattaaaga agctggagta gtaggaatgg
    23281 gtggagcagg attccctact catgtaaaat taaacataga tttaaatggt ggaacaatat
    23341 tagcaaatgc agctgaatgt gaacctttat tagctcataa tataaaagaa atagaagaaa
    23401 gaccagaaat agtttatcaa ggtataaaat atgctatgga agttactaat gctggaaaag
    23461 gtatgctagc aataaaaagc aagcatccaa aagcaataga agctttcaaa aaagtaataa
    23521 agccaggaga caacatagaa gttgcagaac tagttgatat gtacccaatg ggagaagaga
    23581 gagcaatagt aagagacgtt ctaggaaaat tacttgagcc aactcaatta ccttcagagg
    23641 ctaatgcagt agtaataaac gtagaaacac taactagaat agtagaggct gttgagcaaa
    23701 aaagaccagt tatatctaaa aatataactg tagttggtca attaaacagt ggaaaagagt
    23761 ctatagtatt tgaagatgtg ccaattggaa caactgtcgg agaattaatt gaaagagctg
    23821 gtggaataaa aggtgaatat ggtgaaataa tcctaggagg acctttcact ggtaaagcta
    23881 ctactttaga tgctccaata actaaaacta gtggtggtat aatagttact atgccatttg
    23941 ttaatgaaaa gagaaaaatg ggtcttttag tctgtgcatg tggacctaat gaagagagaa
    24001 tgagagatat agcaactaaa atgggagtta ctgatatagt atcagttcaa aaatgtaaac
    24061 aagctcaaga aattaaaggt gcattaaaat gtgaaaatcc aggacactgt ccaggacaag
    24121 ctcaaaaatg tattgaattt aagaaagctg gagcagaagt tatattaata ggaaactgta
    24181 ctgactgtag taacacagta atgggttctg caccaaaatt aaagttaggt gtatatcata
    24241 ttactgacca tgtaatgaga acagttaatc acccattaat aagaagaata aaaggtgacg
    24301 taagattaaa aataaaataa tagataaata gaagttagac atctttagaa gtaaaactta
    24361 aaaattactt ctaaggatgt tttttaatgt attttgaggg caatatatag ctttaaaaga
    24421 aatcaaaaaa gtgccaaatg taaaactaat taaaccatat atatttaaga agggattgaa
    24481 ttggagttgt gaattatgtt agtagaaacc attctaagtg tttgttattg gtacatttga
    24541 tatttgtatg taaatacaga aaaaactttt tgctcagatg gatactttgc tttcagtgta
    24601 ggaaatgttt ctaaagaaat gattgaaaaa tacatacaaa gtaagggata ggtttacatc
    24661 caccaaccta aaggtatagt ggttttacgc cttaatttat aaaaagaaat taatttatat
    24721 tattattaag tttaaaataa aaagtctcaa attgatttat aagtcatttt gggacttttt
    24781 tatgtaaata taatatgcaa atatagtttg tttaaaaata tctattttaa ttatattctt
    24841 aaatttatga ttggatacag gcattcatta attgtttgat gaatagaaaa atgttttgaa
    24901 ggtgaaaata gtattgaaaa aataatatac taagggtata attcatt
//
LOCUS       DAEPOG010000040        24388 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEPOG010000040 DAEPOG010000000
VERSION     DAEPOG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24388
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.040"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(60..1349)
                     /locus_tag="KR432_001378"
     CDS             complement(60..1349)
                     /locus_tag="KR432_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0639923.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1429..2133
                     /locus_tag="KR432_001379"
     CDS             1429..2133
                     /locus_tag="KR432_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBH0639924.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2225..3271
                     /gene="selD"
                     /locus_tag="KR432_001380"
     CDS             2225..3271
                     /gene="selD"
                     /locus_tag="KR432_001380"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2273..2275,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH0639925.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3289..4701
                     /locus_tag="KR432_001381"
     CDS             3289..4701
                     /locus_tag="KR432_001381"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH0639926.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            4815..6698
                     /gene="selB"
                     /locus_tag="KR432_001382"
     CDS             4815..6698
                     /gene="selB"
                     /locus_tag="KR432_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH0639927.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            6803..9541
                     /locus_tag="KR432_001383"
     CDS             6803..9541
                     /locus_tag="KR432_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBH0639928.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(9660..10604)
                     /gene="manA"
                     /locus_tag="KR432_001384"
     CDS             complement(9660..10604)
                     /gene="manA"
                     /locus_tag="KR432_001384"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBH0639929.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            10777..12138
                     /locus_tag="KR432_001385"
     CDS             10777..12138
                     /locus_tag="KR432_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0639930.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12226..14115
                     /locus_tag="KR432_001386"
     CDS             12226..14115
                     /locus_tag="KR432_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0639931.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14130..14585
                     /locus_tag="KR432_001387"
     CDS             14130..14585
                     /locus_tag="KR432_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0639932.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            14644..14961
                     /locus_tag="KR432_001388"
     CDS             14644..14961
                     /locus_tag="KR432_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0639933.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            14986..16248
                     /locus_tag="KR432_001389"
     CDS             14986..16248
                     /locus_tag="KR432_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0639934.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16353..17417
                     /locus_tag="KR432_001390"
     CDS             16353..17417
                     /locus_tag="KR432_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0639935.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17471..18649
                     /locus_tag="KR432_001391"
     CDS             17471..18649
                     /locus_tag="KR432_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH0639936.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(18840..19898)
                     /locus_tag="KR432_001392"
     CDS             complement(18840..19898)
                     /locus_tag="KR432_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBH0639937.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            19965..20336
                     /locus_tag="KR432_001393"
     CDS             19965..20336
                     /locus_tag="KR432_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBH0639938.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20373..21047
                     /locus_tag="KR432_001394"
     CDS             20373..21047
                     /locus_tag="KR432_001394"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH0639939.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21296..22135
                     /locus_tag="KR432_001395"
     CDS             21296..22135
                     /locus_tag="KR432_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0639940.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22378..22474
                     /locus_tag="KR432_001396"
     tRNA            22378..22474
                     /locus_tag="KR432_001396"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22412..22414,aa:Sec,seq:tca)
     gene            22625..23332
                     /locus_tag="KR432_001397"
     CDS             22625..23332
                     /locus_tag="KR432_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBH0639941.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23474..24310)
                     /locus_tag="KR432_001398"
     CDS             complement(23474..24310)
                     /locus_tag="KR432_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0639942.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
BASE COUNT         9672 a         2602 c         4137 g         7977 t
ORIGIN      
        1 ttttagattt ataaggataa gtatattata aattttttat tataatttcc aactacttgt
       61 tactttttca tatttttttt acttctagct ttttttcttt ttttcttctt tatatttgaa
      121 gctattataa ataaaactaa aagcactaga atacctgcaa taatcttaac aaaagtcatt
      181 aaaatattat ttccagtaaa aaatggaaga ctgctatcaa gattattttt tgcaattaag
      241 tctatactgt tttctagttt accattatta tatacattta aagtaccaac cttagctcct
      301 ttttttatag gcaaatcaat tttatttaac tctgttttgg tatcataatt gccttgagat
      361 tcatcttttt ctaagaatat cctataacta ttttttggtt cataaatcaa ttcatgttgt
      421 tttgtaaaca atactttttt agtatctgtg tatttttctt tatcaacaat agtagcacac
      481 ttaaagttat caaatccata atcaatcaga atacgtgagt ctaaatataa atcagcatta
      541 gttgaattaa atacagcaac gataactctt ctgccatctt ttacagcact cgataaaaga
      601 catttaccag cagcatcagt atatccagtt tttataccat caactatatc atattttata
      661 tttataggtt gacctttgta tgtaatctta tcatgtgatg tcaaaaactt attagtgttt
      721 acaaagtatc tctcataagg atatgctttt gtagcttcaa atttaagtga cttagttttt
      781 acaatttgtc taaaagtatc attattcata gcttctctag ctatgagagc catatcatga
      841 gctgttgtgt agtgatttgg gtctggtaat ccatgaggat tattaaaatg agtattagta
      901 gctccaattt tttttgcctc agagttcatg aggtcaataa atttttgtac attgccacca
      961 ccaacatatc tagccaatac aaaagcagca tcattagcag aatgaaccaa aagagcatct
     1021 agtaactgct ttacagtaaa agattcccca ttttttagat acatactaga gccatctatt
     1081 tgaggtaaat ctttaatttc tattacttga tttaaatctt taacctcctt tagaactaaa
     1141 caagctgtcc atactttagt tgttgaagct ggataaagct tagaatttcc atccttatta
     1201 tatagtattt tgccagactc atagtccatt aaaactgcat gtttagcaac tatagctggt
     1261 tcattatctg caaaagagat attagagctt cctaaaaatg aagtaaatat caataaacag
     1321 attaataaaa gtgataaatt tcttttcatt gtgagcctcc aaattttaaa ttcatctttt
     1381 agtatagcat atatttgtca ataattaaaa gaaaggagtg attaatatat gaaaaaaaag
     1441 gtaaaattta tttcgttttt tgttttattt attttaataa tatcatcaat aatcgtttat
     1501 aaatcgctta ttttcaaaga tgatatttat gttgtaagta aaaaaaattt acctaatgaa
     1561 aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata ataatcacaa taaagtaact
     1621 aaagacatag ataaaaattt tcaaaataat aaaataacta tatacataag tggagcagta
     1681 aatagacctg gaattgtaac tatagagtca gacaagagac tttatgatgc tgtagaatta
     1741 ctaggaggaa cgacaaaaga agcagattta aatggagtca atctatcagt aagattagaa
     1801 gatgaacaac attacataat acctaaaata ggtgaagcaa catctgttac aagtaatgat
     1861 gattccaata aaccaaatca gaagaatgag tcaagaggtg agcctaaaaa taaatccaat
     1921 aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag cgactataga agagttagat
     1981 tctctacctg gggttggaga agctacagcc aataaaattt tacaacacag agaagaaaat
     2041 ggacagttta gttcaattga ggaaataaaa aatgtaaatg gtattggaga taaaaagtat
     2101 gaaaatataa aagatttaat ttgtgtagac taaaatataa tacaaaactt acatatagat
     2161 atgttattat aaatatgtat gtatatgtgt gtacatgtgg aaatttagta aaggaggcac
     2221 ttttatgaaa aaagaacaaa aaaaacttac agagatgaca acagcaggtg gttgagccgc
     2281 taaaataggg ccagaggttc tggcatcagt tttatctcaa ttgcctaaaa atgataatat
     2341 agaaaattta ttagtaggac tagatacagc agatgatgct gcagtttata aattaaatga
     2401 tgatatggca cttatccaaa ctttagactt ctttacacca atggtagata acccatacgt
     2461 atttggtcaa attgctgcat caaattcact atcagatgta tatgctatgg gaggcaaacc
     2521 tttggttgct atgaatattg tttgttttcc atcatgccat gatatggatg tacttgcgga
     2581 aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga gcacttttgg ttggagggca
     2641 tactgtagat gacaaagagc ctaaatatgg tctttcagtt tctgggattg taagtccaaa
     2701 taaagtttta tcaaatgcta ctgcaaaacc tggtgataag ttaattataa caaaaccaat
     2761 tggagtgggt gtattaaata cagccatgaa agaaggtatg gtagaacaac atatagcaga
     2821 taaagtaata gaaataatga tacatttaaa taaatatgca gcaatgagtt ttgagaaatt
     2881 tgatgtaaat tcggttacag atataactgg ttttggtcta cttggtcata ctttagaaat
     2941 ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa catgtaccaa ttattgaagg
     3001 tgcaatagaa atggcacaaa tgggaattat acctgctggt atgtataaaa atatgcatta
     3061 tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt gcagttcaag atattttata
     3121 cgacccacaa acatcaggag ggttgttaat ttcagtaaaa gaagaacttg cagaggaact
     3181 tgtaaaagat atgaaattaa atggagcaat agaagctaaa attataggtt ctgtagttcc
     3241 aaaaggggaa aaatatatta cagttttata aaatattata gagaaagagt gattgttttg
     3301 agcaaaagag aattgtttgc aatgttgcct tcagttgatg aagtattaag tgataataga
     3361 attgtagaaa taataaatga atatcctaga agtttagttt tagaatctgt gagagaagta
     3421 atagatttaa aaagacaatt tatattaaga ttaaaagaag atgcatcaaa ctctgtaaca
     3481 atagagtttg gtgaaataat agaatctgca atagagagag ttaagttaaa ttactcatta
     3541 tctttaaaaa aagttataaa tgcaactggt acagttattc atacaaattt agggaggtct
     3601 ttacttagtg aagatataaa agatgaatta tggtgtgctg cttcaaggta ttcaaactta
     3661 gaatacgatt tggataatgg agaaagaggt tcaagatact ctcatttaac tagtacaata
     3721 aaaagactta ctggagcaga agatgtacta gttgtaaata ataatgcagc ggcagtactt
     3781 cttgtactta gcacaatggc taaaggagga gaagcaattg tatctcgtgg agaattagtt
     3841 gaagtaggtg gttcatttag gattccaagt ataatggctc ttagtggtgc agaattagtt
     3901 gaagttggtt caactaataa aactcatttg aaagattaca aggaggccat tacagaagat
     3961 acaaatgtat tgatgaaggt tcatacaagt aattatagaa taatgggatt cacagagagt
     4021 gtttctatag aagaattggt taatttaggt aaaaaatata aactacctgt aatagaagac
     4081 ttaggaagtg gagtttttat agatttatca aaatacggtt tatcttatga accaactgta
     4141 ttggattcta ttcgacaagg tgcagatgta gtaactttta gtggagataa gatgcttgga
     4201 ggtccacaag caggtattat agttgggaaa aaagagtata tagaaaaaat gaagaaaaat
     4261 caacttacta gagcacttag agttgataag cttacaatat gtgctcttga agcaacattg
     4321 agaatgtatt tagatgagac aaaagctata gaaaatatac ctactttaaa aatgttgaca
     4381 tataaaattg aagaattaga agtaaaagca aataaactat ttgaaaaaat aactgcttta
     4441 aacttaaatg caaacataaa tatagaagat ggattttcac aagtgggagg agggtctatg
     4501 cctcttgaaa ctataagtac aaaggtaata tcaataactc ctgaacatat gaatgtttca
     4561 tctttagaaa aaaaactaag acttagtgaa gcacatataa tagccagagt gtatgataat
     4621 aaatatgtac tagatgtaag aactatattt gatgatgaat ttgatgtaat agttgcggaa
     4681 ttgagaaaag catttaatta aataaatttg agctaataaa tattaaattt agaagtataa
     4741 gtatgaagtt ttgattaaaa aatagctaca cacacttgtg actttagtca tagcaggttt
     4801 agagggggca aaaaatgaaa aatgtagtaa ttggaacagc aggtcatatt gaccacggaa
     4861 aaactactct tataaaagca ttgactggaa gagagactga tacattagat gaagaaaaaa
     4921 agagaggtat ttcaataaac ttaggattta cttattttga tttaccaaac aatagaagag
     4981 ctggaattgt tgatgtccca ggacacgaaa aattcataaa aaacatgtta gctggagcat
     5041 caggaattga tatagtttta cttgttgtag cagcagatga aggggttatg ccacagacag
     5101 ttgagcattt agatattcta agctttttaa atatcaaaaa tggaatagtg gttctaacta
     5161 agtctgatac tgtagatgat gaatttagag agctagtaaa agaagatatt tgtgaaaaaa
     5221 taaaaggaac ttttcttgaa gatgctgaaa taattgaagt tgattctata tctaaaagag
     5281 gtatagacac tcttattgaa aaaatagata aaatgagtga tgatatagaa gataaaaatg
     5341 aaaactctcc agcaagactt aatatagaca gagtattttc aataaaaggg tttggtactg
     5401 taataacagg cactttaata gaaggaaaga taagtataga agatgattta gttatatatc
     5461 cacaggtatt aaaaacaaaa ataagaagta tacaagttca tggagaaaat aaagatacag
     5521 cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt aaaagtggat gatataaaaa
     5581 gaggagatgt tttagctgca ccaaactcat tagaggaatc aatgatgtta gatgtaaaat
     5641 tatcattggt aaatcattta gataaaggat taaagcactg ggatagatta agattgtatc
     5701 atggaactag agagatactt tgtagagcag ttccactaga taaagaatta attgaaagtg
     5761 gagaaagtgg ctatgttcag ttgagacttg aagaaagcat agtatctaag aaaggtgata
     5821 cgtttgtagt gagacgttat tctccaatgg aaactatagg tggaggtgta ataattgacc
     5881 catctcctaa aaaacataag aaatttgatg aaaaagttat agaagcatta aaaataaaag
     5941 aaaaaggcga actaaaagat ataatagaag agtacttaaa aaggaattta aaaaattatc
     6001 caaatataaa agaaataatg agctatagtg gtgctcatga agaagatgta aaaagagcgc
     6061 ttgaagcact tatatcagaa gataaagtat ttattatagg aaatatgtat atgcatataa
     6121 atcaatacaa taaattaaaa gaaaatacaa taaagctttt aagtgagtac cataagaagt
     6181 ataggcttag aaaagggata ctaaaagaag aagtaaggtc taagatagag agtaatttta
     6241 agactaggga gatggatatt ttattagaaa aattgtctac agaaaatgct ataaaaatag
     6301 aaaataatat agtatctcta ttagattttg aggttatttt aaatgataag caaaaagaaa
     6361 tagcaaaaaa aatagaaaaa agattaaaat catgtggagt ttcttctata ctgacaatag
     6421 atgaagtttc tgaaggaaat cataactatg ctgaagtatt ggagtcaatg ataggtaata
     6481 aagtagagaa attagatgat ttgtatataa tggacaaaga tatttatgaa aatgcaaaga
     6541 acattttgat aaattatata aaagaaaata aagaaataac attaggagaa tacagagatt
     6601 tgattgattc tagcagaaag aattgcatga ttatattgga aaacttcgat agaaacaaaa
     6661 taactaaaag agtagaaaac aaaagaattt tgttttagca gaaaatgtct gtttttgctt
     6721 taaggtataa agaaggaatt ataatgtcga gtatcgtata attaattagt ataaaatatt
     6781 aattaagagg aagtgtaaag tattgaagaa tataatacct gatgtaaaac cagatataaa
     6841 agaaagagaa gacatgagcc ttttatttat aataatgggc tcagtacttt tattatttat
     6901 tataaaagat atagagaaat atgaaacttt attacatgcc atatatacga tatttttagt
     6961 agctacagga gtaattatat ttaatactac aaaatttaaa gtagattcat tttctacatt
     7021 tttaggatta atttttatag ctactggagt attggagtgt atatatttat ttaattcctt
     7081 ggggattaaa actaagtcta taatggagat taacatcaca atatcagcaa taacagattt
     7141 atttcctata ctgggtgtat acttaagttt taaatttgtt aaggataaca aacaaattta
     7201 ttcaagtata attttattta tagcaactgc tacattaaca ataacatctt tattcataat
     7261 atcaggaatt tgtgactatc tgggaaatgg taaatcacaa tatgctttag gagttataat
     7321 atctatattc ataataatta tttctatcat tagtggaatt gaattaaatt caagttcaaa
     7381 taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac agaataatag taataataat
     7441 tttatcaaga gttccaaatc tattgcatat agttattgat aatagaaatg tagagcggat
     7501 attatcacag ataataataa atgtagcctt atattattta tataattaca tagtatctaa
     7561 gaatattaag aaaacagcac tggaattgca tgatacgaat gaagaattaa caaaaaaaac
     7621 ggaaagttta aaagaaaaga ataaaaaatt aatctatgag acagaaaaga tagaggaact
     7681 taagggaata cttatgaaaa gagaatctag gctacaatct acattagatg tagcagtaaa
     7741 tagcatagta gtgtttagca aagatggaga aattacatat gcaaataaga gctttagaaa
     7801 tacttttgga gactataaag aacaagataa attgactgat aaaataaaaa acttcaatga
     7861 tttgatagaa agcattcatt atgtgtttgt tcatgagaag aatgcagaga aacttattag
     7921 tacaagcgat aataaagttt atcaagctat atttgcacct ttaattattg cttcacaaac
     7981 agaaggagta ttgtgtgtac tagaagataa gactaagaaa aaggagtacg aacgcaaact
     8041 tattgaagca aataagagaa gtaaagattt tttagaaagt attggagatg gcatagtagt
     8101 gcttgaaggt aataacaaaa tatatgttaa taatgcatgt agggaaattt ttaaaaataa
     8161 tttagagagt attgattttt cattagtatg taaaagtgaa gaaagtatag aaaaaagata
     8221 tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt tctcagtata ctaatgctgg
     8281 tactaataaa actattattg taatacgtga tacaacctct cgaaaaatat ctcaaataaa
     8341 acttgaggaa agtcaatcat catattcaag atttatagat atattacctg atggcatatg
     8401 cttattaaaa gaagatttaa gtataaacta tgcaaataag tcgtttttag atatgctagc
     8461 ttttacaaac atagatgata taaaagattc taatattaaa ctattaatga atgcgaatac
     8521 tgaagaaaaa atgaaattta cggataatat ggctaaagtt ttaaatgaaa atagaagtat
     8581 acttttactg gaatatgaat tgataaatag ttatgatgat atagtagaag tagaattaag
     8641 tgcattacct tttgctatat acaatactag atacataatg cttattataa aagatttagt
     8701 acataagaaa tattccgagc aagcagaaaa agaactgctt gaaagattta aaacagataa
     8761 aatcaaaacg gagttttttg caaatatgtc tcatgaatta aaaactccac taaatgttat
     8821 atctagttca aatcaactgg tagattcttt ctatagaaat gaaaagataa aagattacaa
     8881 taacaatatt aaatctcatg tggatttagt tagacaaagt tcttataggc ttcaaagatt
     8941 aataaataac attatagatt taacaaagat ggagtctggg ttctatacac taaaacttgc
     9001 gaaatacaat attgtatctg taatagaaga cttatttatg aatatagaag agtatgcact
     9061 tagaaaagac ataaaaattc tttttgatac agaccttgaa gaaattaatg tttatataga
     9121 caaggttgaa attgagagaa ttatgttgaa tctattgtca aactgtatta agtttactga
     9181 taatggaggc tggatatatg taagtattca ttataaaata gataaagtaa tcataagtgt
     9241 aaaagacact ggagttggaa taccacaaga taaattagaa ttgatttttg aagaattttc
     9301 acaagttgat aagactttat ccagaaatac tgaaggaagt ggaatagggc ttgctatagt
     9361 taagaatcta gtaagtcttc atggtggaga tatagaagta gtaagtgaag tgaataaagg
     9421 cacagagttc ttaatttcac ttccaatgaa gggatttagt agtgaacatt acacagaaga
     9481 taagagaata tataatattc aagaaaaaat aaaaatagaa ttttctgata tatactatta
     9541 aaaatgatta tgagcaaaaa taaaataaaa atactatttg tttaaatatt taagttgtaa
     9601 gaaataggct atcataaggt aaattattat gatagcctat ttttagctag gttttatatc
     9661 tatatgtgag aacatataat ctctaaatta ccttcaaaac taaaatctcc aaagtcgttt
     9721 ggcagtataa aatgcatacc cttttttaaa ttatattcta cagaattgtg tataagtttt
     9781 ccatttccat ccaacacact gtataaagaa aaaggtgtat tgtgtgtaaa attacatttt
     9841 ccaaaaacat ctaatttata aacactaaaa aattcgtttg acacaaatgt tgtgcattta
     9901 aagtctgatg ttgatacgat tttatagtca gtatcgaaat ttatatgagg aacattcgta
     9961 acatctattg atttttgaac atgaagttca cgcttattac ctgaattgtc agttctatca
    10021 taatcataga ctctataagt agtatctgag ttttgttgag tttctaaaat caatgttcct
    10081 ttgcatatag catgtatagt accacttgga acatagaaaa agtctccctt ttttatatta
    10141 acctttctaa gtagtttgtc ccattcatta ttatttacca tatttacaaa ttctttatga
    10201 gattttgcat tatgacctat tataatttct gcatcatcag aacaatctat tacataccaa
    10261 cactctgtct tacctaactc tccattttca tttttcttag catattcatc atttggatgg
    10321 acttgaactg ataaattatc atttgcatcc aatatttttg taagtaaagg aaacttatca
    10381 ccaggtgtgt ttccaaataa ttctctattt ttattccaca attcagatag ttttttacct
    10441 ttatattttc cattttctat taagcaatct ccatttttat gagagcttat agcccaacat
    10501 tcaccagttg ttggcgaatc tatttcataa ttaaatttat ctttaagtgc agtaccaccc
    10561 catattctat ccataaaaat aggttttaaa aataatggtt ccataatcct gttcctccta
    10621 tgatattaaa tttatcctac aaataattat atataaatag attataaaat caatagtaaa
    10681 aaagttttgt cccttgaaat gacataaagt ataaggagtt attaaataag ataaactatg
    10741 atatataata taaagttcta aattggagtg ataattatgg agttgtttaa ggcagaaagc
    10801 aaaaaatatg aaaaagaatt tttaaaatta ttggagcaat gggttagtat accttcattt
    10861 tatgatagaa aaactgtatc taaagatatg ccttttggaa aaggagtata tgatgcatta
    10921 aactggtttg aaaatctagg tagagaaaac aatttcaaag taaaaaatat agataatcat
    10981 gctgttcaaa ttgaatatgg aaatggtaaa gaatatgtag atatatttgg acattgtgat
    11041 gttgtaaatc caggtgaagg atgggatagt gaaccattta aactaaatat tataggtgat
    11101 aaattagttg ctagaggtgt atctgataat aaaggaccta tgatagtgaa ttttttagca
    11161 ctaaaaatga taaaggattt agatattaat ttaaaaagaa aggttagatt aatagctgga
    11221 ggaaatgaag agagtggatt taaatgtata aagcattatt atagcaaaga gccatatggt
    11281 gtatgtggat ttactccaga tgctaaattt cctgtattaa atggagaaaa aggtggagca
    11341 ataataaagt taatttcaaa tatagacgat aaatctttat atataagtgg tggaatagag
    11401 tttaatacaa taccagataa agtttatata aagaatgtag aaaaattggg taaagataat
    11461 atatgttttg atattaacaa tatatcgata aattatgata atggcaatta tattgtgtat
    11521 ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa atcctatatt agctacaatt
    11581 aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag atttatataa gttgataaat
    11641 caagataata taaatggaaa cttatttggt ttaaatattg aggggaaatg tgggatttta
    11701 tcaatagtgc caacaataat aaatatagta gatggaaaat tagaagtagt tttaagcgtt
    11761 agatatccag aaatattaac cattgaagat ataataaaaa aatttaattt gtatatggag
    11821 caaaataata ttaataaatt tgagcttatt ggggaaaatt taaagcaagc aaattatata
    11881 gatagaaatt caaaacttgt aagaagtttg catgatatat atataaagta ttctggagat
    11941 ttgaaaaatg atgttagagt aactagtgca ggaagttatg cttctgaaat gaataattca
    12001 gtaatatttg gttgtgaatt tcctgatggt agctttggca atgtccatag tgcgaatgaa
    12061 tttgctagct tagatagatt tataactgca ataggtatat atgcagaggc aataattaca
    12121 ttatgtaata aaatataaat acaaaatgtc ccttatatag acatattata tatacaatta
    12181 aaattttact tgatatataa ttgaaatcaa agagaaggtg atacaatgat aactaagaaa
    12241 catgcaataa tagtaaagag tttaagcaat aaagatggat atatgacatc aaacgaatta
    12301 gctatcaaat tggatgtgtc tactaaaact attaaaagat atatagcaga tttaaatagt
    12361 gttttaagca aatatgattt ggtaatagct tcttctagag gtataggata caagttaagc
    12421 ggttctaaaa acaatatagc cagagctgtt aaagaagcaa ataaatatat tgatggtttt
    12481 ttagatgact ctgaagagtc aagaatgtca aatataatat gtatgcttat taatagaaat
    12541 tatatgagta tagaagcaat ggcagaagaa ttaaatctaa gtatagccgc tataaataaa
    12601 ttgtctagta agcttaaaag aaaacttgaa aagtatgact tagttataaa atctaaacct
    12661 tactatggaa gctatataca tggtgaagaa attaatataa ggcaattaat tactgactat
    12721 gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt tagatgacat tagtgaaaac
    12781 gatatatact gtatagaaag tattttagaa aagcacttaa gagaaaaaga tacaataatt
    12841 tcagataaag attttaatct tttgctttca aaaataattg tttctgtatt taggagtaag
    12901 agaggtcata gtaacaatat aaacttaatg gatacaagtt atagatttca taactactac
    12961 tttatagaaa atctaatgaa agaaatatct gataaaattg gatttaaact tattgaagat
    13021 gaagtaattt atatttcaaa ttattctggt gtaatagctt ataaaggtac acaaggaaga
    13081 aaaaacacaa gtgaaattga ggaacgaata agtagtgtta taagtagtgc acttcaagat
    13141 atattcctta tttcaggtag tgattataca aaagatgatg agtttatggt tgcaatctct
    13201 gaccatataa agagattttt aaatagagct agagctaatg taaaatcaaa taatccttta
    13261 cttcatcaaa taaaagagaa gtttcctata gcttttaatt tagccgtgtt tttatcaaat
    13321 aaactagaaa ctgagtttaa tttaaaactt gatgaagatg agcttggata tatagctatc
    13381 cactttgctg cttcaaatga aagaatgaaa aaaaatacta gtaagaaaat ttgtatagtt
    13441 tgtcattatg ggataggaac aggacaactt atttctgaaa agctaaaaca gaatataagt
    13501 gatttaagtg ttgtaggtgt ttatccagtt agatatcttg atatggctat aagtcaagat
    13561 gttgatttga tagtatctac cgttgaatta aaaggatatg aaaaacctgt cttatatata
    13621 gaaaatatat ttgatgatag cttaattgaa aacgtaaaca aagcctttta tgaaaaggaa
    13681 gaaagaagaa agataataag taacatgttt gatgaaaaag cttttttcag tataaaggct
    13741 agtactaaag aagaggtcat aatttcactt tcaaataaac taaaagaaag agattttata
    13801 gaggaaagtt ctataaaaag tataatagat agagaaaata tttcatcaac tgaaatagga
    13861 aatctagtag caatacctca cacaatagtg aagggagata aaaaatctat aataggagtt
    13921 ggaatacttg aaaatccaat aatttgggat aagcaagagg ttcagctagt atttatggta
    13981 ttttttaata ctaaagaaaa gcataatttc tcgatattta aataccttta taattttata
    14041 aaagatgaag gaggagtaag aggaattatt aaaatatgtg actttaataa attaatggca
    14101 ctgataggta attaaggagg aaattaataa tgggaacaaa catatttaac aaagaatatg
    14161 tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt aaaatttata tcaaaaaaag
    14221 ctaaagactt aaatttggca gaagatgaga gcttagtata tgaaggttta atggctagag
    14281 aagaacaatt tactacaaat ttaggagaat ctatagcaat acctcacact aaaaatgatg
    14341 caattgaaaa tccagcagta gtagtattga agtttaatga agatgttgtt tggaatgaag
    14401 gtgaggataa ggttaaatta gcaataagct tacttatgcc aggaaaaagt aaagagaata
    14461 ttcatcttaa attattatca tctctttcaa gaaaattaat aaataaagaa tttaaagatt
    14521 ctcttttaaa aagtgataat gttgaagaaa tatctaactt aataaatgaa gctttaggtt
    14581 tataattaaa tactaaggtt ataaaataaa aaaataatta actaaaaata ggaggaattt
    14641 gaaatgaata aaaaattagt agcattatgt gcttgcccaa tgggactagc tcacactttc
    14701 atggcagcag aagcaataga gcaagcagca aaggcattag gttatgaagc aaaagttgaa
    14761 actcaaggtg ctgatggtgt acaaaatgag ttaacaagag atgatatatt aggagcaact
    14821 atgataatac atgcagtagc aataacacca gaaggtatgg aaagatttga tggttgtgaa
    14881 gtttatgaag ttgaattaca agaagctata aaaaatgcag aaggtgttat aaaagaaata
    14941 gaagaagatt taggaatata ataaatttat tgtggaggaa atagaatggc aattaagaaa
    15001 aaagttataa acagttcagg tgcttctaca acaggaggaa ataatgtagg aaaatctact
    15061 ccatctagtc caactcaatc aaagggaaat ggaaaatgga aagaaataag taaacatata
    15121 atgacaggta tatcatatat gataccagtt ttagtaatgg gtggacttat aggggcctta
    15181 tctcaattaa taccatatgc tatattagga cttgacccat ctgtaggtat agttgatgct
    15241 atgaactcag gagaatttac agggttcaag cttagtcttt taaatatagc acaattaatg
    15301 tcaaatttcg gatttacttt gtttggattt gcgataccac tgtttgcagc attttgtgca
    15361 aactctatag gtggaaaaac agctttaatt gcaggattta taggtggata tatagctaat
    15421 aaacctgtag gtgtaccaca attcattgat ggacaatgga ctgaagttgt tccagttgca
    15481 tcaggatttt taggtgcaat actaatagca ttcataatag gatattttgt aaaatggtta
    15541 aataaatcta taaaagtttc tcataactgg ttagctttta aaacaacatt tttaatacca
    15601 ttaatagcgt cattagcttg tatggtattg atgatattta taataactcc atttggtgga
    15661 ttaattaatg aaagtatgaa aaacttctta acagcagcag gagcagctgg agaatatgta
    15721 tatgcaacag ctttggcagc agctacagca ttcgacttag gaggaccaat aaataaagca
    15781 gcaggattcg ttgcacttgg tttaactaca gagaatgtac ttccaataac agcaagaaca
    15841 atagctatag ttgttcctcc aattggatta ggattaacaa cattattaga taaaagatta
    15901 gtaggaagaa gagtatatga tagacaattc tatcaagcag gaaaaacttc tatattctta
    15961 tcatttatgg gtatatctga aggagcaata ccatttgcac ttgagagacc aggttttgtt
    16021 atacctttaa acatagttgg aagtgtaata ggagctatta caggtataat tctaggagca
    16081 attcaatggt tcccagagtc agctatatgg gcatggcctc tagtagataa cttatttgga
    16141 tatataatag gtatagcagt tggtgctata ttcatagcag taggaaatat attctataga
    16201 aataaattaa taaaagatgg taaacttgtt gtagattata tcgactagta tttaattttt
    16261 actatctata aataactttg gctggtaata tggttttaat caatattacc agccttacct
    16321 atatgattaa ttaaaggcgg tggagataag atatggataa gatagaaaaa gtaagagagt
    16381 attttagaga atatgatata gatggatttc ttataaatag tcctacaaac aagttttatg
    16441 ttggaaattt atttagctct tcaggttatg tttttataac gaaagaatca caatatataa
    16501 tagtagattt tagatatttt gaagaaatta aaagaaaatc tagtttgttt aatgtagtat
    16561 taatggacaa aactagaact cattttgata taattaatga tatttgtaga gagcagaata
    16621 taaaggaaat tggatttgag ggaaatgaag tatcttttga tttatataga agtatgagta
    16681 ataagttaag tgcaacctta aaatcagttg atttatctac attaagggaa acaaaaaatg
    16741 aagacgaaat aaaatatata aaaaaagctt gtgaaatagt tgatgctaca ttttatcata
    16801 tagtagactt tataaagatt ggtatgactg aaaagcaagt tgaaaatgaa attgttagaa
    16861 ttataaaaga tttaggtggt caaaaagaat ctttcgatac aatagttgca tctgggctta
    16921 gaggagcatt acctcatgga aaagcaagtg aaaaaattat agagtatgga gattttgtta
    16981 cttttgactt tggtgctaag tataataatt attgttcaga tattaccaga accatttgta
    17041 tgggtactat aaataaagag ttagaagaaa tatataatat agtaagaaaa gctaatgaag
    17101 agtgtataag agttttaaga ccgggaatga ctactggaga gatagataaa gttgcaagag
    17161 acataattgg ttcatatgga tatgccaata attttggtca taatctgggt catggtgtag
    17221 gaattatggt tcatgaatat cctgccttag ctcctgaatc aaatgaagta ttaaaagaag
    17281 gaatgattgt aactatagag cctggtatat atgttccaag tcttggaggc gtaagaatag
    17341 aagatgatgt actcataaca caagatggat gtatgagatt gactacatct actaaggatt
    17401 taatagttgt aagctaaaaa taaattagat aaatatttta taacaataag aaaagaagag
    17461 gtaaatttaa atgtataatt ttgatgataa accagataga gtaagtgaaa agtgtagaaa
    17521 atgggattta aatattataa gagataaatt tggagatatt agagaagatt ttgtaccaat
    17581 gtggattgct gatatggatt ttaaaatacc aacagaaata gaaaacaaat ttattgaagc
    17641 tgtaagaaga ggtgtatttg gatatacata ctgttatgat gaattttatg attcagttat
    17701 tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa gaatggatta cattaactta
    17761 tggaactgta tcaacacttc attatgtagt acaagctttt tgtaagaaag gtgatagtat
    17821 aatattaaac acaccagttt atgacccctt tgaatcatca gcaaaaaaac aaggtgtaaa
    17881 tgtaatttgt aatacattag atgttgtaaa tgatagatat tatataaatt ttgataaatt
    17941 agaagttcaa attaaagaaa ataagcctaa attaatgatg ttttgtacac cacataatcc
    18001 atctggaagg atatggacaa ttgaagaaat gacaagggta gcaactatat gtaaagaaaa
    18061 taatgttatt ttggttgcag atgaagtaca tgcagagcat attcattatg gtaaatttaa
    18121 ttcaatactt aagattggaa aagagctttt agaaaatgtt attctactga catcaccaaa
    18181 taagggcttt aatcttggtg gattaaagac ttcttattca atagttataa ataagaatat
    18241 aagggataaa tttagaaata aattaaagca aaattctata acttctccaa atgtgtttgg
    18301 aataatagga ttgataaccg cttataatga atgtcatgag tggttaagtg gtgtaaatga
    18361 atatataaaa tcaaattatg aacttttaga aacttgggta aataaatata ataagataaa
    18421 attgatgaaa atggaatcat cttacttagc ttggatggat ataagtggat taggtataag
    18481 tgcaagtgag tttacagata aattggctat agatacagga gtattattag aagatgggag
    18541 tcattttgta aaagatggtg agaagtatgt tagaataaat ttaggaactc aaaaggaaaa
    18601 tgtaattgaa gctttaaata gaatggattt atttttaaag tctttataaa aatttggttg
    18661 aattagtatt ttatataaat ttaagctatt aatatggtat atacataagt agaatttgtt
    18721 ttagattact taagtatata ccatatttta tataaaaatt aaatttgcat tagtcatgtg
    18781 ataactattt aaattcatat gtattttgta tgtataatag atataattat tatttaaact
    18841 tattgtttat ttaatttttc aatagaaaaa gatttatctg taagtttaaa attatctttt
    18901 atacccttag ttaaatatac tttcttatcc ttagttatga atatagcatc tacattatca
    18961 atttcattaa ttaaatccat acctttttca agaccaagtc caaatgtaga agtggataag
    19021 gcatcacaat taatagattt atcagaaatt attgtaacac tacttagatt gttttcaaaa
    19081 ggataccctg taaatggatt taacatatga tgatatattt ttccgtcttt ttcgataaat
    19141 ctttcataaa ttccagaagt aacaacagat ttatttgtgg tttctatgtt tcctatggat
    19201 tcaccatttt tactagtggg gtcttgaatg cctattttaa aaggtttatc atttttacca
    19261 tcttctaaag taaatatatt tcctcctaag tttactaaac ctttttttac gtcctctgat
    19321 tttaaatagg ctacaatttt atcagcagca tatcctttag ctatggcacc taaatcaatt
    19381 ttcatatttt ctttggttaa ttttatagat ttattttttt catccaatat gatgttctta
    19441 taatcaataa gaggcagaag tttttgtatc tcagaatcac ttggaacttt agctttatct
    19501 gttccaatag cccataaatt tgataatgga ccaattgtta tgtcaaaata tccatctgat
    19561 aatttggaat attgtatagc ttcttttacg acgaaaaatg tatcatcaga aactctgaca
    19621 aattttttac cagcattatc atttatttta gaaacatcac ttcctggtat atgagtgctc
    19681 attttgttgt caatatatct taaaattgaa tcacattcat ttaagatttt atcacttttg
    19741 gattctttta cattaaatat agtaacttcg tttactgtac caaggtagta gctagtttta
    19801 ctatatttag tattctggct tgaactccta aaaaatgaaa ataaaattaa tgctaaaatc
    19861 aatatcacaa aaataaatgt gatttttttc ttattcatgg tatattcctc ctatgaaatt
    19921 gtgttattat tttattataa cataatttca taggaggaca aaaaatgaag aaaaaagaca
    19981 ttcttcttat tttaggcatt ttggttgtta ttatagcatg ttatgggata ataaatgtta
    20041 taaattctaa aaatgctgga aatattgaga tttatgtaga taataaattg tataaaactg
    20101 tttcaataaa tgccaaagaa gaatttaaaa tagagaatag aggggggtat aacatagtaa
    20161 agattcatga taaaggggtt gagatagtag atgcttcatg ccctgataag gtttgcgtac
    20221 atactggatt tataaacaag ccaagtcaaa gcatagtttg tatacctaat agagtgagca
    20281 taaaaataaa tacaaatgaa aagaatgata atcaagaaga tataatttct aaataaaata
    20341 ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa cttaggaaac gattgggatg
    20401 aattattgaa agaagaattt gaaaaagatt actacttaaa cttaagaaaa tttcttatag
    20461 aagaatataa aacacgccaa attttcccaa atatgcacaa tatatatgaa gctttaaaac
    20521 acacttcata caaagacaca aaagtcttaa tattaggaca agacccttat catggagata
    20581 atcaagctca tggtttagct ttttcagttc aaccacaagt gaaaactcca ccatctctat
    20641 taaatatgta taaagaatta aaagatgatt taggttgttt tatacctaat aatggatatt
    20701 taatgccttg ggcagaccaa ggagtgttac ttttaaatac tgctcttact gttagagcgc
    20761 atgaagctaa ttctcataag aacaaaggat gggaaatttt cactgataga gtaatatcta
    20821 ttttaagtga gcgtgaagac ccagttattt ttgtactatg gggaagtaat gcacgtaaaa
    20881 aagtagagtt gattgataca agtaaacatt atattttgga agcacctcat ccaagcccat
    20941 tgtcagcaag taaaggcttt tttggatgta aacacttctc taagataaat gaaattttga
    21001 aaaagctagg aaaagaacct attaattggc agatagaaaa tatataaaag taaaaggtaa
    21061 caaaatgata acaaaacaac aataaacaat gacattgttg taactaatag aaatatgctt
    21121 aaatagttga aaatactatg tatgcatatt tctatttttt tgtcaagttg acaactcttg
    21181 aaaaaatagt tacaatttag ttacacaaaa aacacaaata aaaaaagaaa cttacaaagc
    21241 agaattttat gaagcaaaat atatagtgta aaaaatttta gggaggaaat tttatatgaa
    21301 ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca gttgttttat cagttggaat
    21361 attatcggga tactctgcgc ttaataaaca agtaacatta gtagtaaggg gagaagaaag
    21421 aactatatca acatttagtt ctaatgtagg agacctttta gaatctcaaa acattagcta
    21481 tgatgcaaat gatatagtaa ctttagatga aaatacaaag ttatctaatg gagataaaat
    21541 agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact aaagacattc catttgaagt
    21601 tacagttgtt gaagatggct ctttattaaa gggtgattct aaggttaaag aagaaggaaa
    21661 gtctggtaaa agtgaattaa tatataaaga aacttatcac aatggaaaga aagttgaaaa
    21721 ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat aaggttgtaa gcaaagggac
    21781 aaaggtagaa atgcaagtag cctcatcaag gggagaaagt tcaagaagaa tggcttcatc
    21841 atcatctagt agcagtagta gtaacaatgg taaaaatatg agagtggtag caactgcata
    21901 tactggtcat agtataactg caacaggaac aaaacctaag tggggaacaa tagctgtaga
    21961 ccctaaggtt ataccatatg gaactaaagt ttatatacct caatttgaca tggtgtttac
    22021 tgcagaagac tgtggtggag caataaaagg aaataaaatt gatatctaca tgaatgattc
    22081 aaaaacggtt tataactggg gaagaaaaac tatagatata cgtatattaa aatagttaaa
    22141 taatttatat ttttactgga aattatattt tgattatata gagaagaaac tctttataga
    22201 ttcttaaaat ataatttcca gtttttattt tggatatgta ataaaacagg taaattttaa
    22261 ccatgtatag ataaaagcta tttaaattat aatttactat cagataaatc tataaaaatt
    22321 gttgcaaaga gtcaaaatat atgatattat agatttcgtt gagaaggatt tcattatggg
    22381 agtggatggg tgccggtgtg ccctctagtc ttcaaaacta gtatgagggg ttaagagcct
    22441 cttaggtggg ttcgattccc acgcattccc gccaatatgc aaaaggtatc ttttaattaa
    22501 aagatacctt tttaaattta ttttaagata atttattttt aataatatat aatgttttgt
    22561 attatataat gtaaattttc taatactatt atatatttag ataaaaatct aggagtgtgg
    22621 aaagatggaa aataatttta caacgagaac tagttttcta gttggagatg atgggataga
    22681 aaaacttaat aattcaaata taatagtatt tggagttgga ggagttggta gttttactgt
    22741 tgaagcttta gcaagagctg gagtaggcaa tattactatt gtagattttg atgatgtaga
    22801 tataaccaat ataaatagac aaataccagc cctacattca actgtaggaa ggtacaaagt
    22861 tgatgtcatg gaagaaagaa tattagatat aaatcctaat atcaatataa aaaaaataag
    22921 aagtctttat aataaagata ctagtgacga aatactaact gaaagatatg attatgttgt
    22981 ggatgcgata gacatggtaa gttccaaaat acatctaatt gaaacttgtg aaaaaaaagg
    23041 gttgaaaata ataagttcta tgggtatggg aaataaatta gacccaacta aaatagtcgt
    23101 tacagatata cacaaaacaa gtacttgtcc tctagctaaa gttatgagga aagaacttag
    23161 ggatagaggt atcaaaaagt taaaagtagt atattctact gaacaaccaa tagaacttaa
    23221 gaagaaagta atgaatggta gaaaagtaac acctggtagt gtatcatttg tcccatctgt
    23281 aggaggttta ataatcgcat ctgtaatagt aaatgaacta ttaggacaat aagttttaaa
    23341 tattttaatt tataaatttt atatacaaat ttgaaggcta gaagcatgaa tataaagcaa
    23401 ttttatattc atacttttag cctaattatt ttatattacc ttattttatg tagttgtagt
    23461 tttatttttt agattatgta tctatttcat aattatattt tgtaaatata atttctccat
    23521 cactataaaa aacaatatct cctattctat ctgttctata aattgataca tggttttgtt
    23581 ttagattatt aataacctcc ctatgtggat gaccatattg atttttatat ccacatgata
    23641 ttgcagctat atctggacta atttttttta taaattctaa agatgaagaa gaattacttc
    23701 catgatgacc tatttttata aaattaatat tatttaattt aaaagaatgt aaaatttctt
    23761 tttcattttc ttcttcagca tcacccataa ataaaaaaga attatcgtta aaagttaatt
    23821 tgaaaactat agaatttaaa ttagattctt cttgtatata tgatggactt aatacatata
    23881 tatttatatt gttatctatg tttaaaacat catttttata gagatgctga atagataagt
    23941 ttttatccgt acaagaattt attagatttt gataagcctc actatcagtt gattgttcag
    24001 gcatatatat ggagtttaca ttaaattttt ttataacatt atcaagactt cctatgtggt
    24061 ctgaatctgg atgagttgct attataatat caatagtttt tattcttttt tgtctaagat
    24121 aactaataat tatattttcg ctatcttcat cacctccatc aattaatata ttcttatttg
    24181 tgggtgtttg aactaaaata ctatcacctt gtccaacatc aattatatgt atagatagta
    24241 gagatttttt gtcacagccg attaaaagtg atattataat aagtattgat aaaaaaattt
    24301 tatttctcaa taaaaaatca ctccattcat tacatgttta tataaattta tgatgggtaa
    24361 tataagatat atatgactta ttatattt
//
LOCUS       DAEPOG010000041        24301 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEPOG010000041 DAEPOG010000000
VERSION     DAEPOG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.041"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            109..300
                     /locus_tag="KR432_001399"
     CDS             109..300
                     /locus_tag="KR432_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBH0639943.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KR432_001400"
     CDS             455..1864
                     /locus_tag="KR432_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0639944.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="KR432_001401"
     CDS             1995..2684
                     /locus_tag="KR432_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH0639945.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KR432_001402"
     CDS             2687..3622
                     /locus_tag="KR432_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH0639946.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KR432_001403"
     CDS             3619..4512
                     /locus_tag="KR432_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBH0639947.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KR432_001404"
     CDS             4590..5336
                     /locus_tag="KR432_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBH0639948.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KR432_001405"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KR432_001405"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH0639949.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KR432_001406"
     CDS             6193..6897
                     /locus_tag="KR432_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBH0639950.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KR432_001407"
     CDS             complement(7085..7513)
                     /locus_tag="KR432_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0639951.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KR432_001408"
     CDS             complement(7550..8980)
                     /locus_tag="KR432_001408"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH0639952.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KR432_001409"
     CDS             9112..9696
                     /locus_tag="KR432_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBH0639953.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KR432_001410"
     CDS             9712..10233
                     /locus_tag="KR432_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBH0639954.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KR432_001411"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KR432_001411"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH0639955.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KR432_001412"
     CDS             12596..13366
                     /locus_tag="KR432_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBH0639956.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="KR432_001413"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="KR432_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH0639957.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="KR432_001414"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="KR432_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH0639958.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="KR432_001415"
     CDS             14727..15812
                     /locus_tag="KR432_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBH0639959.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="KR432_001416"
     CDS             15832..16701
                     /locus_tag="KR432_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH0639960.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="KR432_001417"
     CDS             16694..17560
                     /locus_tag="KR432_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0639961.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="KR432_001418"
     CDS             17601..18314
                     /locus_tag="KR432_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0639962.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18315..19484
                     /locus_tag="KR432_001419"
     CDS             18315..19484
                     /locus_tag="KR432_001419"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH0639963.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="KR432_001420"
     CDS             19488..20324
                     /locus_tag="KR432_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0639964.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="KR432_001421"
     CDS             20325..21320
                     /locus_tag="KR432_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0639965.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="KR432_001422"
     CDS             21338..23026
                     /locus_tag="KR432_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0639966.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="KR432_001423"
     CDS             23038..24105
                     /locus_tag="KR432_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0639967.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9698 a         2709 c         4343 g         7551 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttatttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttagaatttt
     1321 caaagataaa gttgattcaa atattaatat atatgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaaatttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttagtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aacaatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata cgtttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc ttcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agttgggaac atttttacat gatataggta agcctgatag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatac tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagcg ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagctcca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa aaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaagg gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaattaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggaa atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatgttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct ttttttatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagacag tttgttttta tttgtttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagtggtt tattatatta ttaaagagaa gtaaacgaaa
    13681 ggtagattaa ggaggctaaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaatac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttacttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcgt ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcgcttac agtgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctacca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt gtagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttaaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gagattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcattg actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggtgatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgtgga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatgt ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagatcat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtga ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggaacttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatggc aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 ggattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccaa
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagtataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaaaaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttttaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaaaattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctttggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaagtctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggctcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaaa agtacactgc
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga cgttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa tataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatata atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc tataaatatt ccattctata aagtattttt
    22741 taatataaca gttccgatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat atacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22921 agtattagtg ctattaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggaaaaaatg
    23041 aaatttaaaa agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23641 aaagcatggc agtatatgga taagttgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa aattaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgcaatga atgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaaaaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 a
//
LOCUS       DAEPOG010000042        24070 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEPOG010000042 DAEPOG010000000
VERSION     DAEPOG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.042"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            64..381
                     /gene="flgB"
                     /locus_tag="KR432_001424"
     CDS             64..381
                     /gene="flgB"
                     /locus_tag="KR432_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH0639968.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            389..793
                     /gene="flgC"
                     /locus_tag="KR432_001425"
     CDS             389..793
                     /gene="flgC"
                     /locus_tag="KR432_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH0639969.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            803..1120
                     /gene="fliE"
                     /locus_tag="KR432_001426"
     CDS             803..1120
                     /gene="fliE"
                     /locus_tag="KR432_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH0639970.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1145..2695
                     /gene="fliF"
                     /locus_tag="KR432_001427"
     CDS             1145..2695
                     /gene="fliF"
                     /locus_tag="KR432_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH0639971.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2708..3778
                     /gene="fliG"
                     /locus_tag="KR432_001428"
     CDS             2708..3778
                     /gene="fliG"
                     /locus_tag="KR432_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH0639972.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3771..4502
                     /locus_tag="KR432_001429"
     CDS             3771..4502
                     /locus_tag="KR432_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH0639973.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4506..5825
                     /gene="fliI"
                     /locus_tag="KR432_001430"
     CDS             4506..5825
                     /gene="fliI"
                     /locus_tag="KR432_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBH0639974.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5857..6303
                     /gene="fliJ"
                     /locus_tag="KR432_001431"
     CDS             5857..6303
                     /gene="fliJ"
                     /locus_tag="KR432_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBH0639975.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6331..7536
                     /locus_tag="KR432_001432"
     CDS             6331..7536
                     /locus_tag="KR432_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBH0639976.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7554..8162
                     /locus_tag="KR432_001433"
     CDS             7554..8162
                     /locus_tag="KR432_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBH0639977.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8189..9172
                     /locus_tag="KR432_001434"
     CDS             8189..9172
                     /locus_tag="KR432_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBH0639978.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9185..9388
                     /locus_tag="KR432_001435"
     CDS             9185..9388
                     /locus_tag="KR432_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBH0639979.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9388..10206
                     /locus_tag="KR432_001436"
     CDS             9388..10206
                     /locus_tag="KR432_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBH0639980.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10196..10891
                     /locus_tag="KR432_001437"
     CDS             10196..10891
                     /locus_tag="KR432_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0639981.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10903..11346
                     /locus_tag="KR432_001438"
     CDS             10903..11346
                     /locus_tag="KR432_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBH0639982.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11361..11735
                     /locus_tag="KR432_001439"
     CDS             11361..11735
                     /locus_tag="KR432_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBH0639983.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11736..12401
                     /gene="fliP"
                     /locus_tag="KR432_001440"
     CDS             11736..12401
                     /gene="fliP"
                     /locus_tag="KR432_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH0639984.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12414..12683
                     /gene="fliQ"
                     /locus_tag="KR432_001441"
     CDS             12414..12683
                     /gene="fliQ"
                     /locus_tag="KR432_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH0639985.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12695..14509
                     /locus_tag="KR432_001442"
     CDS             12695..14509
                     /locus_tag="KR432_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBH0639986.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14518..16593
                     /gene="flhA"
                     /locus_tag="KR432_001443"
     CDS             14518..16593
                     /gene="flhA"
                     /locus_tag="KR432_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH0639987.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16568..17584
                     /gene="flhF"
                     /locus_tag="KR432_001444"
     CDS             16568..17584
                     /gene="flhF"
                     /locus_tag="KR432_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBH0639988.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17581..18459
                     /locus_tag="KR432_001445"
     CDS             17581..18459
                     /locus_tag="KR432_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBH0639989.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18473..19174
                     /locus_tag="KR432_001446"
     CDS             18473..19174
                     /locus_tag="KR432_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBH0639990.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19190..19612
                     /locus_tag="KR432_001447"
     CDS             19190..19612
                     /locus_tag="KR432_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0639991.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19597..19782
                     /locus_tag="KR432_001448"
     CDS             19597..19782
                     /locus_tag="KR432_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0639992.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19823..20596
                     /gene="flgG"
                     /locus_tag="KR432_001449"
     CDS             19823..20596
                     /gene="flgG"
                     /locus_tag="KR432_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0639993.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20617..21378
                     /gene="flgG"
                     /locus_tag="KR432_001450"
     CDS             20617..21378
                     /gene="flgG"
                     /locus_tag="KR432_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0639994.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21401..22282
                     /locus_tag="KR432_001451"
     CDS             21401..22282
                     /locus_tag="KR432_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0639995.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22285..22659
                     /locus_tag="KR432_001452"
     CDS             22285..22659
                     /locus_tag="KR432_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0639996.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22691..>24070
                     /locus_tag="KR432_001453"
     CDS             22691..>24070
                     /locus_tag="KR432_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0639997.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKV"
BASE COUNT         9953 a         2577 c         4539 g         7001 t
ORIGIN      
        1 atagcaactt cattttttat taataagcaa aataaataaa atatattttg aggaggagtg
       61 agtgtgaata catatgacat tataaaaatg ggattagatg caactaattt aagaagtcag
      121 acaatagcaa ataatatagc caatataaat actgaaggat ataagagaca ttatgtaact
      181 tttgaagaaa cattaaatga tgctatgcca aaaatagaag aaaaaacaga caattctaaa
      241 ggattaagag tggatggcaa taatgtagat atagaagaag aaaaagtaaa tcaagcaatg
      301 gcaacattac aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgactaaa
      361 tcaataataa gtgggaggta attaaaaggt gagtgtgttc agtggaatga gaatatctgc
      421 tagtggatta acagcagaaa gcttgaaaat ggatatcata tcgtccaatg tagctaatat
      481 aaatgccaca agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga
      541 aaactatgat aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa
      601 atcacctctt aaagaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt
      661 agaatatcca aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc
      721 atatgattct aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga
      781 aataggaaag tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc
      841 aaagttagat aatttgattg gagaaacaaa accattaact cttaataata aagaagaaaa
      901 aaagaacttt gaagatgtaa taatagatac tatagataaa gttaattttc aacaagtgac
      961 agcagatgat atgaaagaaa aatttataaa aggcgaagat gtttctatgc atgaagttat
     1021 gttaataggt caagaagcac agctatctat gcaatattta atagaagtta gaaataagat
     1081 tcatgatgca tatcaagaaa tgaataaggt acaaatttag ccttttgaac aggagtaata
     1141 atcaatgata gccagtttta aaaataagat taaagagcaa gttgctaaat ctaggacaaa
     1201 gataagttct ctaaaaaagg gaactaaaat agcattaggt ataggggtta ttgctatatg
     1261 tttagcgcta gtatttacag caatatctgc gagcaaaaat aagtatgcag tcttattttc
     1321 agaactagat gttcaagatg ctgcagatat atcagcagaa ttagagtcta aaaagataga
     1381 tactaaggta aagggaagta cgatttatgt acctagaaca gaagttgata agttaaggct
     1441 ggagctagca ccaagtctta caggtggttc taaaggattt gcactaatgg atgatagttc
     1501 atcaatagga cttactgatg aagagtttca gattaagaag caaagaatga tacaaggtga
     1561 aatagaaaaa acaataaaga gttttccaca agtggaagat actagagtac atataacatt
     1621 tggagagtca tcagtatttg agaaggaggc tattccaggg agtgcagcag tttatttgat
     1681 gttaaaacca gggacgactt tggaaaaaaa ccaaataaaa tctgttatgg cacttatatc
     1741 aggaagtgtt acaaatatac cagagaaaaa tgttcaagtc attgataaca aaatgaattt
     1801 attatctgaa ggtattttag aggatgaaga tgttgatgaa aataatcaac caacaaatag
     1861 tttgattaat gttgataaaa ataaggcatc tgaacaagca ttgaataaac aattagaaaa
     1921 gtctatttta gaaatgctag aaccaatatt tggtaaagga aaagtaaaag ccacagtaaa
     1981 tgctgattta agttttgata cagctgaaaa aactgagata aaaattgacc ctgataaagt
     2041 tgcaataaag gagacaaggt cgaaaaattc atctaaatct gctacaaata atgttcaagg
     2101 tgtagatgct aacatgaata accaaggaaa taacaatggt actaatacag aagatagttt
     2161 agatgagagt tttgagtatg aaacaggtaa gattgaaact cacactatag ttgcgcctgg
     2221 agaacttaat agattgacag cttctgttgc aatagatgga aaaatagcta aaggtgtaca
     2281 agctgatgta gaggatattg ttaataatgc tataggtatg gatggagcga ggggagatac
     2341 tcttagcgtt gtttcaatgg tatttgaccc agaagggaag gcagaagcta aacaagagct
     2401 agaagagatt aaaaaagaag gatttaaaaa gaacataata aaagcagtta ttggagtagt
     2461 ggcagtggta gcaattggag ctattggata cttaatatat aagaaaagaa gtgaacgaga
     2521 agatatggat tatgaagatg aagattatat ttctaaatct gattctgttg ataggaagga
     2581 taatcctcta ttaggagatg acccagaact gacattagag gaagctgtta aaatgtatgc
     2641 agaagaaaag ccagaccaag taacagagat aataaaaact tggttaaatg agtaaggggg
     2701 atagaaagtg tccacggaaa ttacaaatgt taatgcgttt gacttaagtg atataaatga
     2761 aagtgataag aaagagaaga tagaaggtga gggtttaaaa aaagctgctg tattattaat
     2821 gacattagga ccaaatgtgt ctagtgatat aataaaaagc ttgcctgata aacaagttca
     2881 aaaaataggt ttagaaattg caaatatttc ttcaatagct tcaaaagaaa gaagagaaat
     2941 cttaaaagag tttgtagaga taaatagggc aaaagattat gttatagaag gtgggcttga
     3001 atatgcaaga gagttgttaa gcaatgcttt aggaaatgct aaagcaaaca aattacttga
     3061 aggtatatca ataaatacaa gtacaaagcc ttttaaacat attataaaac tagatgtaaa
     3121 gaaaatatta gaaatactta gggatgaaag tgcacaaact atagctgttt tacttgcaca
     3181 tatggagcca gaaaatggag cagaagtttt atcatactta gattttgatt tacaaaatga
     3241 agtagctatg agaataagct caatgggttc aatatctcca gaagtaataa aggtaattga
     3301 aagttcatta gaagataaat tacactcaat aagtggtgat gattttaagg attatggagg
     3361 agtgtctacc ttagttcaaa tattgattaa ttctgataga aaaacagaaa agaatatttt
     3421 gggaactatt gaagaacaag acccggcttt agcagctcaa attaaatctc atatatttgt
     3481 ctttgaagat attgtcaagt tagatgatat aagtatacaa aaagtattaa aagaagtgac
     3541 tatgaaagat ttagtatatg ccttaaaggg tgtggatgat gagatttcag atgctatata
     3601 tagaaatcaa tcttctagag cagcagatgc acttaaagaa gaaatggaaa tggttggtgt
     3661 cataaaagtt acacaggttg aggctgctca acaaaagata gcaaatatag taagaaaact
     3721 tgaaagagaa ggtgccataa ctattgtaag gaattttggt ggtgactttg ttgaataata
     3781 agattattaa aagtaaaaaa gcatcatata aaggagtgct agatttaacc ataatagata
     3841 aagttagagt gggagaatca aatgtaaata aagaaaatga ttgtagattt agagatgagg
     3901 taataaaaga agctatagaa tctgctgaga taaaaaagaa tgagatatta gaagaagcaa
     3961 aaatgaatgc acaaaatata gaaaaacaag catatgaaaa agggtatagt caaggtcaaa
     4021 aaaatggata tgaagatgga tataaagagt catatgatga atatacccaa aaagctaaag
     4081 atgaagcaga ttttataaaa aatcaggcag acttaatact attacaagct aatgagaaga
     4141 tgactgagta cataaaagaa aaaaataaag agattgtaag acttgctatt aatatggcta
     4201 gttctgttct aaaaaagcat ttcgaagatg aagaatctat gagtgatttg ttaaagcaaa
     4261 ttattcaaga ttatgaagga agtagttcat ttatagtaag atgtaattct ttctataaag
     4321 ataatataga gaaagagttt gaattattca aagaggaaac atcactaaaa tcaagtgtat
     4381 ttgttattgc agatgatagt atagataaag gcaatgctat catccaaaag gaaaatggaa
     4441 gaataatagt tggtatcgat tgtgctatgg aaaaactaaa agaagaattg cttggtgaat
     4501 aaagtatgta taaaactgat atatctatag attttgacaa aataaataag aaaatagaaa
     4561 atttagaact cactatatca gaaggtagag taaagaagat aataggactt actgtagaag
     4621 ttgaaggaat aaaagctttt gttggggagt tatgtgttat atacaatcaa gtcaataaac
     4681 cagtgaattg tgaagtagtg ggttttaaag ataaagaagt catattaatg gcgttgggag
     4741 agctaacatt gatagctcct ggttgtaaag ttatttctaa gggaatacct ttaagtgtta
     4801 tgtgtagcga taatttacta ggtaaagtac ttgatggtct tggaaatcct atagacaact
     4861 cagatgctgt tttaggagat agatacaact tgaacaatga acctcctgac ccaatgaaga
     4921 gaaaaaagat acgaaatata atggaaacag gtgtaagagc aatagatgca tttactactt
     4981 gtggtgaagg acagagaata gggatatttg caggaagtgg agtaggtaag agtacgactc
     5041 taggtatgat tgctaggaat gcaaaagcag atgtaaatgt aattgccctt attggagaaa
     5101 gaggaagaga ggtactagat tttattgata aagatttagg tgaagaggga atgaaaaaat
     5161 ctgttgttgt atgtgcaaca tcagataaag ctcctttggt taggctaaaa ggtgctctta
     5221 ctgctactgc aatagcagag tattttagag accagggaaa aaaagtaata cttatgatgg
     5281 attcagtaac tagatttgct atggcacaaa gagaagttgg tatagcaata ggagaaccac
     5341 cagctcaaaa aggatataca ccatctgtat ttgctatttt accaaaactt atggagagaa
     5401 cagggacatc agataaaggt tctataactg cattttatac agtattagtt gatggagatg
     5461 actttaatga gccaatagca gatacaacaa gagggatact tgatggtcat atagttttat
     5521 ctagagactt ggcaaataag aatcattatc cttctataga tgtattgaac agtttaagca
     5581 gacttatgaa tgagattgct tcaaaagaag acataaaaat agcatctttt gcgagagata
     5641 tgcttgctga gtatagagaa gcagaagatt tgataaatat aggtgcatat gcttcaggta
     5701 caaacaaaaa aatagatgaa gcaatctact atcatgagca tattattaat tttttaaaac
     5761 aaggaataaa tgaaaagagt tcttttaatg aaactataag tagtttaaga agaatctttg
     5821 aataaacaat ttaaaaaaat atttgaatag gttgtgatgg aattggataa aaattttaag
     5881 tttagattac agaaagtttt agacttaaaa ataaaagatg aagaagagat aaaaatggaa
     5941 tttgctaaga ttcaacaaaa aaaaatagat atagagagca atttagaaaa cttagaaagt
     6001 aattacagca aatattctat aagtaaaaat aatgatagtg ttcaaaatca aaaaataact
     6061 attaactatt tgttagcttt aaacaactct ataatggatt tatcagaaga gctggataag
     6121 tcgacaaatg aacttgaaaa agctagaaag cagcttataa gcaaacagat agagagaaag
     6181 tcattagaaa aattaaaaga aaaaaaatat ggacagtatt ataaagaaga acagttaaaa
     6241 gaacaaagca ctaatgatga gtttgcaagc atgagttatt tgagaaatag acaagttcta
     6301 taaaatagta ggaagtgagg atattattgt atgaagatac aagcagattc ttataatatt
     6361 attaaagata ttgggaatag aaaaagctca tctaagaata aaaatataaa aaataataaa
     6421 tttgaagata atctatataa ggcaacagac cataaaaagg ttaaagatag aagagaaaca
     6481 agtaagcaaa atgttagttc agataaacgg gaaaatagtg ataatgagtg tcatttagat
     6541 gaagataaaa gtaatcactt agaagatgaa aatacatatg aaaaagattg tagtaataat
     6601 aatgaagctt tacatcaatt actgaatctt ttaaaagata attacaaaaa agataaagat
     6661 ataaacacta tattgaataa tctaaaaaaa ctaagtatat cagatgaatt aaaacaagaa
     6721 cttaagctta ataaaaatat attaaataag attgcaaatc aaaatttaga gttaaataga
     6781 gctagtacaa ataaccctaa tgatatagaa aatttagaca taatgaagtt aagagaactt
     6841 ttagaaaata atgctagtga agatattgaa gtaattagta aaactacaga aaatgatact
     6901 aaaagcatag acttgtacag ttttgacagc aatagaatag atactcttaa ctcaaataaa
     6961 cagaaagatg atagttctga tattttggaa aaactagcag gggtaaatgg aaataagagt
     7021 ggaaattttg gacaagtaat aaataaaagc gttgatatga ataaaattaa aaatgataat
     7081 gttaatttaa aacagataga atcaaatgta atggatgatt ctataaaagc tattaagcat
     7141 atgaaaacaa atgatataca agaattgaca attaaattaa gaccaaaaga attaggagat
     7201 atgaacatac aattgttaaa agatggtgaa agtatgagag ctgttgtgac tgttttcaac
     7261 aaagatgtct ttgactctat aaataaaaac atagcagatt taaagcaaca tcttgaactt
     7321 acaaatgtaa atataaaaga tgtatcagtg caaatgcata gcgataatac aaatacatct
     7381 gatacctttg ataaagcatt tgaacaacag aatagacaaa ataaccaaga aaacatgagt
     7441 aagcaaaata caaatgataa gcgtaacaat gtagtcatag aagatgagat taaagaaaat
     7501 attattgatg atgatagagt agatttacta gcttagtaag ggggaagtat tgcatgtacg
     7561 gaatagcaac ggctttggca ggaactcagg taaactatag gtcaaataat ttaagccaac
     7621 ttgaaagaaa tattaatgat attgccaata aacaagttag aagtaaagga aatcagacag
     7681 taacagacaa tgggactcca attataggac aaggcgatga tgctgataaa gatttgttta
     7741 tgaagattct tgtagcacag atgggtaatc aagacccaat gaatccacaa gacccaacag
     7801 aatatgtatc acaattagca cagtttgcaa ctttagaaaa gatgtcagct atgaatgata
     7861 agctagatat acttttagcc ttaagcaatt ccactctaat aaattctgct ttgagtatgg
     7921 caacatcatt aattggtaaa gaagtagaag tatctgggga agaggaagaa gatgaaaaca
     7981 ttgtaggagt agtaaaatcc acatatataa aagatgggga agtacactta gaattagaag
     8041 tagatggaag tgacaaaact gtaggtgtta aatatgaaaa attacttaaa ataggagagg
     8101 tagatgcaaa ttcaggagaa gaaaatgact cttcagaaca agaatctcca gaagaagtat
     8161 aatattaaat gagaaaagag gtaaatttat gataaaagca atgtattcag gtgtaagtgg
     8221 aatgaaagca aatcaaacaa aactagatgt aataggtaac aatgtagcca acgctggaac
     8281 tacttctttt aagaaaagtt cagctagaat aacagattca ttttatcaaa ctgtactata
     8341 tgcaagtgct ccaacagcgg cattaggggg aacaaactta ggacaagtag gtgtaggtag
     8401 taagatatca agtataaata aagatatgac acaaggaaat gtacaaccaa ctaataggcc
     8461 atctgattta atgatagatg gagatggata tcttccagta gtaagacaag gaacagttat
     8521 gtatacgaga gatggttctt ttaatttaga tacaggattt aaagcagctg atggtgcttt
     8581 gaagaatatt aaaggtggaa gacttgtaaa tggtgatgga tatatgttac aaggtgtgat
     8641 ttatgatggg gaatataaag ctgatactgg agaattcatt aaaaagggtg atgctaaagg
     8701 aaaagagcct attgtaatac cacttcaaag tataagtcct aaggatggaa cagttcaaaa
     8761 tgtactagag tataatatat caaaggatgg aacagtagaa tttttattga gtgatggtca
     8821 aagaactcaa tttgtctatg atatacctga tggtgctaag gtaggagata cacctgctgg
     8881 aatagataaa ttaaaaggta caatacaaaa agtgcaaaca tatgcattcc aaaatccagc
     8941 aggactagat gcagaaggag gaaacttatt caagccatca gcaaactcag gagatccaca
     9001 aatagcagga gctacaggaa acataataca aggggcaata gaagcttcca atgtagatat
     9061 agcagaagaa tttacagaaa tgataatagc aagtaggtct ttccaagcaa acagtaaaat
     9121 aatatcgaca tcagacgaaa tacttcaaga aataataaat cttaagagat agtaggtaca
     9181 tattatgata agagtaacgg atttaaacgg aagaagatac atattaaatt cagaccttat
     9241 agtgagaatt gactctgtac cagaaagtag gattctcctt acaacaggag aaaaaaatct
     9301 tgtaaaagag tcagtagatg agatagtaga taaagtaata aagtataaaa gaaaagtaaa
     9361 tgtaggttat attaggagtg aaaaataatg aaaaaaacag attcactaac attcattgga
     9421 ctaatattat ctacagtatt agttttattt ggggcagtaa aaggaagtag ttcaggattg
     9481 aaaagttttt tcgattttgc atctatatta atcacagtat ttgggtcatt tggagctttg
     9541 atgattacat ttactattga tgatataaaa cttataaaag atgcgctaca atactcattc
     9601 aagacaatga atatatcgaa attagactta ttaaatcaat ttaaaacact ttctaaaaaa
     9661 gcaagaagag aaggactttt atctatagaa agtgatgtaa tgaaaataca agacccattt
     9721 atgaaaaaag ggttagagtt atgtattgat ggccttgaag taaatgaaat aaggtctata
     9781 ctagaactcg aaatagattc tgttgaagaa accaataaca gatattctaa actatttaaa
     9841 acttggggga ctttcgcacc tgcatttggt atgttgggaa ctctaatagg tttaatacaa
     9901 atgttatctg accttacatc tccagattta atagcatcgg gtatgggaaa agcattaatt
     9961 acgacatttt atggttcttt acttgctaat atcgctttga acccaattgc atataatata
    10021 gatgagaaga ctgaaaaaga gatttatgta aaggaaatga tgctggaagg tataataagc
    10081 atacaatctg gggagagcag catagtagtt gaggaacgtt tagctacata cctttcaaac
    10141 aatgagaagc tagaaattat gaaatccaat aaaaatacag aaagagcaat gagtaatggc
    10201 gcgtaaaaaa aataagaaaa aggatgagat aaatccagat gcatggttgg ctacttatgc
    10261 agatactata acactgattc ttacattttt tgtgttattg tatgctacat cagttgtaga
    10321 tatggaaaaa tttaaaaaca tagcagatgc gttaaaaggt cagttttctg ggataagtat
    10381 gttcagtaca ggttcaagta caagtgagaa gtctccaata gagagtgttt tagtggataa
    10441 aaatgataag tcaaatttag aacaggattt aaaagataaa gttaataatt ctaacttaca
    10501 aaatagtgta acagtcaaag atgatagtag agggatttta ctagaactag atgattcaat
    10561 tttatttgac tctgggattt ctgaattaaa agcagatagt aaagccactc ttaataaagt
    10621 atatgacatg ataaagatga tgaaaaataa gatagttata gaaggccata ctgataatgt
    10681 acctgtaaaa tcaagtacac atgaatccaa ttgggattta tcttctagta gagcagtaag
    10741 tgttgtaaga tactttgtag aagaaaaggg catggaccct agactatttt cggcaacagg
    10801 atgtggagag tatcaacctt tggtttcaaa tgaaacagca aagggaaggg ctgagaatag
    10861 aagagttaat atacttataa ctattgacta ggaggaagag gtttgttcaa aaataaaaaa
    10921 attattataa ttattttgat actgattatt ttctcagcag gagcaggttt ttttatatta
    10981 ggagaaggtg gaaataaaca aattgacaag attgcaggtt tgtttattcc aacagggaaa
    11041 ggcaaagatg aagtaaagac cgaaaatctt gacttagaag agatggtatt aaagctagct
    11101 gatgaaggtg taagatattt aaaagtatca ataactgtat cttatgattc ttcatataaa
    11161 gatatagaaa aaaatacgcc tttaattagg gataatatta taaattgttt tatggcaaaa
    11221 agggcagatg attttactgc tgataattta aagaacataa aagaagaaat taaaaataca
    11281 ttaaatatac aactaggtga aaatgtaatt caaaatatat atttcacaaa tatagtagtg
    11341 caataaaagg atggtaagac atgatacttt atttactaaa aatattaata tgtatcccaa
    11401 taattttaat attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat
    11461 ataatgataa atatataaaa atactagata ttgtaaatat aagtaaaaac aatagcataa
    11521 taattttaaa aataggtgaa gaaggatgca ttatggcatc ttcttcctct agcatgaata
    11581 aaataaaaga tttgaccaaa gaagaaatat taagaataga aaacgacatt aaagaaagaa
    11641 attttaaaat tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat ttcagtagat
    11701 atattggaaa atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt
    11761 tggctgatgt accatataca agttcaatgc aattgttttt atttctaact gcattaatgt
    11821 ttttaccatt tgtaatgttt atgatgacaa gctttgtcag aatagttata agtttatcat
    11881 ttttgaaatc agcattagga gcacagcaag caattccaag ccaaattttg gtagggcttg
    11941 caatagtatt gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat
    12001 tacaaccata tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagagggac
    12061 ctattaaaga atttttatta aagcaaacca gacagacaga tttagattta tttgtagaac
    12121 aggctggttt gaaagaaaag aaattaactc gtgaaaatat accactatct gttgtagttc
    12181 cagcatttgc tataagtgaa ctaaaaactg cttttcaaat aggtttttta atatatatac
    12241 catttttgat aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt
    12301 tgcctccagt catgatatca ttaccgttta agttattatt atttgtaatg gtggatggat
    12361 ggaacttgat agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg
    12421 aatttatgat aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc
    12481 cagtactcat tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc
    12541 aaatccaaga gcaaactttg acttttgtac caaaactaat agcaattctt ttagtagtag
    12601 ctattggtgg aagctggatg ttacatactc ttattagctt tacttcggag atattcaata
    12661 tgatgtctct tataactaaa taagtaggat atatatgatt tttgtattta ttagaatagt
    12721 ggcattcttt ggaacactaa ggatactatt tccagctggt acacctgctt tatttaagtc
    12781 gctctttgca atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac
    12841 aaatatagac aacattgtat tgtttacctt atatggtata aatgagacca taactggcat
    12901 tatgcttgga tatataacca atctatgttt ttatagtatc agaatggcag gttctatgat
    12961 ggatcaacag atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac
    13021 aactttaatt gataatctta tgaactggac agcacgtatg atatttttta gcatggatgg
    13081 tcatcatgtt ttaattagag gcataaggta tagttttgag ttaattccaa tagggaaatc
    13141 attcgtagat aataatatag attatataat aaacatattt gtgcagtgct ttttaactgg
    13201 attcaaaata gcaataccta ttgtactttg tctgcttatg gcagatttta tattagggct
    13261 tatatcaaga agtgtacctc aactaaatgt aatgataatt ggtatgccat taaaaatact
    13321 ggttggtatt gcgttattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt
    13381 attaagtcaa ataccaaaga tgtatgaggg aactttcgca ctagctccta tgttttttat
    13441 gggttcaaca gataaaacag aagaggctac tccaaagaaa aagggagaac aaagaaaaaa
    13501 aggtaatata gcaaagagta aagaattacc cgttgcaatg actctacttg cattcacttt
    13561 attagtacct acattatttt catatgtagt ggatacactt aaatcttctc ttaattattt
    13621 tttgagtcta gatttttata tgaatataaa ttattcaagt atagagaaat tagttatagc
    13681 aggattaatg gattttttta agatattttt accaatagct attccattct tagttttagg
    13741 tatagtcgca aacctatttc aagtgggaat actgtttact ggtgaaactt taaaacccaa
    13801 tttatcaaaa ttaaatccag ttagtggatt taaaaacatg ttttctatga gaagtttgtc
    13861 aaccttaata aaggatacag cgattatatc tattttggct tacataggat atacattctt
    13921 ccaagataat tatttagata tattaaagct aggtaacata tacctgccaa ctttaatgta
    13981 cacagttaaa gatttagtat attcaatatt aagtaagatt tgtgtagcta tgatagttat
    14041 agcagttgca gattatgttt atcaaagata cagtcataaa aagcaactta gaatgacaaa
    14101 acaagaagtt aaagatgaat ataaaaactc agaaggagac ccagaaataa aagctaagat
    14161 aaaacaaaaa caaagacaaa tttcgtctca aagaaccatg caagcagtac caagtgctac
    14221 tgtaatagta accaatccca cccacttatc tatagcagtg agatatgaaa aagggaaaga
    14281 ccaagctcca gtagtggtgg caaaaggtgc agattattta gcttttaaaa taagggaaat
    14341 tgcaaaggga aatgacatac ctataataga aaataaacct attgctaggt tgttatataa
    14401 gcaagtagaa atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt
    14461 agtagcagta tataaaatca aaaatagata caaagtcctc aaaaggtagg ataagtaatg
    14521 aataagttta atttaaagga aaatatggac gtaatagtgg catttggtgt tgtagggatt
    14581 gttcttatga tgatagtgcc gttacctaca tttatattag atattttact ggcaattaac
    14641 ataagtttat ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta
    14701 tctatatttc caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca
    14761 tcaactagat tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt
    14821 tttgttgtag gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt
    14881 caatttgttg ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg
    14941 ttagatgcca tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata
    15001 gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggc
    15061 tctatggatg gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca
    15121 gcaataaaca taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg
    15181 ggtgcagcac agacatatgt aagacttact ataggggatg gtcttgtaag ccagatacct
    15241 gctctattaa tatctacatc agcgggtata atcgtaacaa gagcttctac tgatgaaaac
    15301 tttggtggat tagtaagtaa acagttaacg gcaataccaa aagcagtaat gatgactggt
    15361 tgtgttctaa tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta
    15421 ggtataggtg ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa
    15481 gacttggagt ttgaaaaacc tgatatggta aatccagata aaaatgaagt agaagatgta
    15541 acaaacttaa taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcttta
    15601 gctgatgaat cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt
    15661 gcaatagaca tgggtatagt cgtgcaaccc ataagaataa gagataattt acagttaaat
    15721 ccaaaccaat acaatataaa aataaaggga aatgtagttg gtgggtatga gataatgcct
    15781 aatatggttt tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca
    15841 attgagccta cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca
    15901 gagataaagg gttatacagt tgtagattca tccacaatac ttataactca cttactagat
    15961 ataataaaat caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat
    16021 gcagcaagag aaaattatag tgctgtagta gatgaattga taccagattt aatgacactt
    16081 ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggatagg
    16141 gttacaatac ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt
    16201 actgaatatg taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat
    16261 aagacaattg tagtggctac attgtctcca gaaactgaag agttggtatc taataattta
    16321 caaaggtctg taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt
    16381 gagagtatac aagaagtaat gaacaatgta tatttcaatc ataatatacc agtaatagta
    16441 gtatcaccaa agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg
    16501 actgtattat cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg
    16561 agcatatatg atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc
    16621 caaaatggaa ttgggtgaca atattacatt aatagataaa aaagaagtaa gaaagtctgg
    16681 aatacaaggt atcttttcaa agaaagatat tgaactaact attggatggg aaaagagaga
    16741 aaacgtagaa caaaaagatt taaaacgaga gatagaacaa ttaaagttaa ttattaacaa
    16801 tatggggttt gataataaga atgataatga tatagataaa atatgtaaaa atctactaag
    16861 tttagaatta aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt
    16921 caatggaatt gatacaagca aaaatttagt agaaatttta aagaaaaaga ttaaaataga
    16981 aaatcaagca ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac
    17041 tacaatagca aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat
    17101 aactatagat acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat
    17161 gaatattcct tttaaagggg taataagtcc cgatgaaatg gaattggcac ttgatgagat
    17221 gaaagattgt gatgttgtat taatagatac aacaggtaga ggctataaaa actctatgca
    17281 aatactagaa atcaaaaatc ttatagataa agctgaaact gataatatac atttagttgt
    17341 gaattgtaca acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa
    17401 ttttaaaagc ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa
    17461 cataatgaat tatgcacaaa aaccaatatc atatattaca acaggacaaa atgttcctga
    17521 tgatattata aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat
    17581 atgatagacc aagctcaaat tttgagaaaa atggcaatgg aaaaaagagg acttgatgag
    17641 tttatagtag agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt
    17701 gttggaaaga gtaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa
    17761 gttcttatat tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat
    17821 gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca
    17881 cagactaagt atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac
    17941 tttacagaag ctcaaagaaa taagtttata catagcatag aacagattca cgatgtggat
    18001 tttataataa ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt
    18061 agtactgaat ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt
    18121 ttattaaaag ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg
    18181 gtaattgatt tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa
    18241 tttctaaacc ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta
    18301 tgtgtaaaaa aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt
    18361 attatagcca tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa
    18421 agttttctaa gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag
    18481 agaagaatta ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat
    18541 accagggaaa ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga
    18601 tgcaataaat aaatttgatg ctgaaaaggg ggcaaagttt tctagttact cttatataaa
    18661 aataaaatct gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct
    18721 aactaaagta aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga
    18781 gccaacttca tatgaaattg caaaagaatt aaatgtttca gaaaaagaac ttcatgatat
    18841 cgaaagtaat atagacatgt taaatattgt atcattaaac tatgtaatct ttgaagatac
    18901 aaatgaaact gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat
    18961 agaagaagaa gaaaagttag agattctatc taaagcaatt agtaatttaa atgaaagaga
    19021 aaaattaata ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt
    19081 gctaggtgtt tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag
    19141 aaacaagata aaagaactta aatattctat atagatggtg attgataaga tgatacaaat
    19201 tatattaata gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga
    19261 atcaaaaaat aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa
    19321 tgatagatta ataattgcag aaaaaagaag attcttttta gacaaagtac ttgctgaaat
    19381 aagaagtaat aatcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga
    19441 aagaaaggaa aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc
    19501 tcttaaagtg aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct
    19561 agatattgga aagggggaac tgtcattaat aaggagttgt tacaaaatat aaggatatta
    19621 ttatgtgata aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgc
    19681 ataatgcttt ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg
    19741 ttaggcatga aatatacaag tgagatgaaa gttttagaat agatgatact gtttagacat
    19801 ttacaaaaaa ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat
    19861 aactaatcaa caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg
    19921 atataaaagt aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa
    19981 taactacgca aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc
    20041 tatggatgaa actatcacaa attttaatca aggacctata aaaactactg ataataaaat
    20101 ggatgtagct attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt
    20161 ttacactaga aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg
    20221 gtattatgta cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa
    20281 tcaaaaattt gaagtggcaa aagataatac aatatcacta gatggaaatg ctatgtataa
    20341 attcaatatt gtagatttta atgactacaa taacttaaaa aaagaaggag ataacttgta
    20401 ttctggagga ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc
    20461 ttctaatgta gatatggtat ctgaaattaa taatatgatg accatatcaa gagaatatga
    20521 ggcaaatcaa aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat
    20581 tggttcagta agataggtga taggaggtga atttttatgt acacaatgtt ttatactagt
    20641 aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat
    20701 tctgattcaa caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta
    20761 ctaaatagac catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag
    20821 gcaacagctc ccatgagaat ttatgaacaa ggtgcattgg cccctacaga tagtaagagt
    20881 aatttggcga tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca
    20941 tatacaagaa atggtggatt taatatagat ggtttaggta agattgtaga tgataatgga
    21001 aatatattag atgttcaatt tgatgcagga tataactata ataatacgaa tatagatagc
    21061 acaaacttaa cagtttcaag aaatggcgag ttattcgtag ataataaaaa aataggaaaa
    21121 atcaatttat atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc
    21181 gtcgcaactg atgcagcaca gattaaacaa gttgagaaag tagatatagt tcaaggctat
    21241 agagagaggt caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga
    21301 gcatttcaaa tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagctaat
    21361 aatttaagag ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta
    21421 cgattttggc aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt
    21481 agctgaattt tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag
    21541 tatgattgtt gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc
    21601 ttcatgtgta gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat
    21661 agatagaagt gtagctgata tgtggattga tataacttct ggaggaactg gagttataaa
    21721 ggataatgat agagagctta cagaacttga taaaaaagtg cttttacatc ttatggatga
    21781 tttagtaaga aatatgcact tatttgaagg ttttgaaaat atacaagtat tggatacaca
    21841 tacacatatt aatttaccac aattatgttc tccaactgca ccagtatgcg ttgtagattt
    21901 aaaagtgcta aatgataatg agtatattgg aagtgtttct ctttgttttc cgtataatgg
    21961 tttagaggct ctattagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga
    22021 tgattcagag gaatttacca aaaaaatcta taatagtgta tctaatatag aattatcagt
    22081 tattgcagag ataggaaaag tatcaattaa tgttgaagac ttacttaaac tagaagttgg
    22141 agatgtaata gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc
    22201 aaaatcatat acagcaacac caggatttat atcaagcaaa aaaggtgtaa aaataaatga
    22261 tgctgttgga aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca
    22321 aagatgttat taaatgataa agttgaaagt gaagttgatg gggatgatga gttagcctta
    22381 gatgtctctg aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta
    22441 tctgtagtta tagggagaac taagatgact ctaagagata ttttaaattt acataaaggt
    22501 tctcttattg aactgaatac tttagcagac caagatgtgg aaatactaat agataataag
    22561 gttttggctt atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt
    22621 atagtaagtg aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt
    22681 aaggcatgag gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact
    22741 caataaaaaa gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg
    22801 tatatcaact ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga
    22861 tatattaaat ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga
    22921 aagtttctat aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat
    22981 gcttacatct gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat
    23041 aaataaagga atgtataaag aagctataca agggtttaaa cttataactg accaatactg
    23101 tgatttaata gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat
    23161 aaaatttgat gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa
    23221 tcaagaaaat aacacattag atatgtataa tagtgctaat atacaaacta aaagtaatgt
    23281 aaaagtagat gattataaga atgagttttt acataaaagt agtagtaacc ataaaattaa
    23341 tgattataat aatgtaacta aagaaaaaag ttcaagcaaa aaccagaaaa gttcagattc
    23401 taataagaaa aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat
    23461 attattagta ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat
    23521 atctaaaaat aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga
    23581 aaataatata gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa
    23641 aaacattaat aaagaggctg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga
    23701 taaagattct gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc
    23761 agaaaaaaaa actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa
    23821 taaagggaat acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga
    23881 ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc
    23941 ttattttgat gaatcttatt ataatttagg gatgatgtac tataacaatg gtgatttaaa
    24001 aaattctaaa ttgacattaa agaagttagt taataaggta ccaaatagta tgtataataa
    24061 ttcaaaagta
//
LOCUS       DAEPOG010000043        23875 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEPOG010000043 DAEPOG010000000
VERSION     DAEPOG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.043"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..265
                     /gene="nusB"
                     /locus_tag="KR432_001454"
     CDS             <1..265
                     /gene="nusB"
                     /locus_tag="KR432_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH0639998.1"
                     /translation="TVCKALKENHDKIDELINKHAKNWTVDRMPKVDVSILRLSVCEI
                     LYLDTPNKVSINEAVELAKIYCDDKSPKFINGILGSVVDEIGK"
     gene            249..1319
                     /locus_tag="KR432_001455"
     CDS             249..1319
                     /locus_tag="KR432_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBH0639999.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            1316..2521
                     /locus_tag="KR432_001456"
     CDS             1316..2521
                     /locus_tag="KR432_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH0640000.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            2523..2738
                     /gene="xseB"
                     /locus_tag="KR432_001457"
     CDS             2523..2738
                     /gene="xseB"
                     /locus_tag="KR432_001457"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH0640001.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            2741..3628
                     /locus_tag="KR432_001458"
     CDS             2741..3628
                     /locus_tag="KR432_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBH0640002.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            3658..4095
                     /locus_tag="KR432_001459"
     CDS             3658..4095
                     /locus_tag="KR432_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBH0640003.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            4129..5994
                     /locus_tag="KR432_001460"
     CDS             4129..5994
                     /locus_tag="KR432_001460"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH0640004.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            6127..6939
                     /locus_tag="KR432_001461"
     CDS             6127..6939
                     /locus_tag="KR432_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBH0640005.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            6959..8638
                     /gene="recN"
                     /locus_tag="KR432_001462"
     CDS             6959..8638
                     /gene="recN"
                     /locus_tag="KR432_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH0640006.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            8853..9719
                     /locus_tag="KR432_001463"
     CDS             8853..9719
                     /locus_tag="KR432_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640007.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(9810..10301)
                     /locus_tag="KR432_001464"
     CDS             complement(9810..10301)
                     /locus_tag="KR432_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0640008.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(10362..10802)
                     /locus_tag="KR432_001465"
     CDS             complement(10362..10802)
                     /locus_tag="KR432_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0640009.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            10963..12021
                     /locus_tag="KR432_001466"
     CDS             10963..12021
                     /locus_tag="KR432_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBH0640010.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            12163..12987
                     /gene="spo0A"
                     /locus_tag="KR432_001467"
     CDS             12163..12987
                     /gene="spo0A"
                     /locus_tag="KR432_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBH0640011.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            13400..14521
                     /locus_tag="KR432_001468"
     CDS             13400..14521
                     /locus_tag="KR432_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640012.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            14539..15414
                     /locus_tag="KR432_001469"
     CDS             14539..15414
                     /locus_tag="KR432_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBH0640013.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            15438..16130
                     /locus_tag="KR432_001470"
     CDS             15438..16130
                     /locus_tag="KR432_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0640014.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            16138..16962
                     /locus_tag="KR432_001471"
     CDS             16138..16962
                     /locus_tag="KR432_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0640015.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            16967..18034
                     /locus_tag="KR432_001472"
     CDS             16967..18034
                     /locus_tag="KR432_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBH0640016.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            18036..18572
                     /locus_tag="KR432_001473"
     CDS             18036..18572
                     /locus_tag="KR432_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0640017.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            18640..19245
                     /locus_tag="KR432_001474"
     CDS             18640..19245
                     /locus_tag="KR432_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBH0640018.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            19257..20171
                     /locus_tag="KR432_001475"
     CDS             19257..20171
                     /locus_tag="KR432_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0640019.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            20213..21385
                     /locus_tag="KR432_001476"
     CDS             20213..21385
                     /locus_tag="KR432_001476"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH0640020.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            21432..22244
                     /locus_tag="KR432_001477"
     CDS             21432..22244
                     /locus_tag="KR432_001477"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH0640021.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            22448..23773
                     /locus_tag="KR432_001478"
     CDS             22448..23773
                     /locus_tag="KR432_001478"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBH0640022.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         9634 a         2382 c         4393 g         7466 t
ORIGIN      
        1 tacagtatgc aaagcattaa aagaaaatca tgacaaaata gatgagttaa taaataagca
       61 tgctaaaaac tggacagtag atagaatgcc aaaggtagat gtttctatac ttagattgtc
      121 tgtgtgtgaa atactttatt tagacactcc taataaagta tcaataaatg aagcggtaga
      181 acttgcaaaa atatattgtg atgataagtc tcctaaattt ataaatggta tattgggaag
      241 tgttgttgat gaaattggga aataatataa taattggaat tgatactagc tgctatacta
      301 cttctatagc agctatctct ttagataaga aagttatatt caatgaaaaa ataatgttag
      361 aagtaaggga taattcaaaa ggtttaaggc aaagtgaagc tgtatttcaa catatcaata
      421 atttagggat tcttagtgat aggataaaat cttttaaaga taagtttaat gtagaaggag
      481 tttgctcatc aaaaaaaccg agacctgttg aaaactcata tatgcctgta ttcaatgtag
      541 gtcataattt tggaaaatta ttgtctagta tatatggttg taggttttat gagactactc
      601 atcaagagaa tcatattgaa gcaagtttat taaatagtaa attaaaaaat aataataaat
      661 ttatttctgt tcatatgtct ggtggtacta cagagatact tttaacaagc aaacaagata
      721 gtcatcacaa tgtatgtgat actaatttag gtaaaattgc caaaataagt attaaaaaag
      781 atgataaaag taagttatac aataattttg gatataatat agatataatt ggaggaagta
      841 aagatattag ctttggtcaa ctcatagata gagtaggaat taagcttgga tataaatttc
      901 cttcaggaaa gtatttggat gaaaatgctt taaattgtaa tcttaaaata gaaagtggtc
      961 tgaaaacttc tgtaagagat gggtatatga atttatctgg attagaaaat caggtaaata
     1021 agattataaa tgataatgga gataatacta atcaaaaaga atacatatct aaattagtat
     1081 tagattcagt agttagaaat atgtttaaat ctttagtata tttatgtgaa acttataatg
     1141 taaatgaagt agtatttgct ggtggagttt ctgcaagtaa gtatattctt agagaattaa
     1201 gcatgaagtt aagaaaaaag catatagaag cttattttac agaaccacaa tattcaactg
     1261 ataatgctgt cgggtgtgct ataataggat tgaataactt tttaggagaa agagtatgaa
     1321 actaagagct ttagatataa gtgaagcaaa ctcatatatt aaaagaattt taataaacga
     1381 cccaatactt tctaatctta aagttaaagg agaaatttct aattttaagg ttcatagtag
     1441 tgggaatgta tatttatcac ttaaagatga aacatcaaag ttaaactgtg taatttttaa
     1501 aagtaatttt aatcgcaatt taaaattaga taatggtgta aaaattatag ctaatggata
     1561 tatatcagtc tatgaaagag atggtgcgta tcaattatac attaatgaaa ttgaaataga
     1621 gggaatagga aatttacaca tagagtttaa tagattaaag gaaaaactta ataaagaagg
     1681 attatttgac cctaaataca agatacctat acctaaaatg ccaaactcaa ttggcgttat
     1741 aacatctcca acaggtgctg ttataaggga tataataaat gtaataaaaa gaagatatcc
     1801 gaaagttaat ataaagcttt atccagtaat ggttcaagga gataaatcag ctgaagaaat
     1861 ctgtgaagca attagatttt tcaatcatat gaagaatgtt gatacactta tagttggtag
     1921 aggtgggggt tcaatagaag aactttggtc ttttaatgat gaaatggtag caagagaggt
     1981 ttttaattct caaattccga taatatcagc agttgggcat gaaacggatt ttactatttg
     2041 tgactttgtg tctgatatga gagccccaac gccatcagca gcagctgaga ttgctactcc
     2101 atcattggat gatataaact ataaacttgg aaacataaaa agtaggatga gtaagtctct
     2161 tacaaatcaa atagagctag accaatatag gttagaaaca gtatttaata agataaataa
     2221 ctatttagat tcgtacacaa taaaagataa agttatacaa ttagacaaaa tatatgataa
     2281 aataattttt ggaatagaaa ataatttaaa attagaagat gaaaaattag taaaaattgg
     2341 agcattactc cataatttaa gtccattagc tactatggat agaggttata gtataactca
     2401 aaaaaatgga aaagtaataa atagtataaa aggattaaaa ataaaggata gtatagatat
     2461 agtattaaaa gatggaaatt tagaatgtat gatagataaa attgaaaata aagagggata
     2521 gtatgaattt aacctacgaa gaagcttata aaagattaga aagtatttta agtgaattag
     2581 aatctaaaaa tgctagcttg gatgaatctt taagcctata tgaagaaggt ataagccttt
     2641 ataaacattg taataagctt ttagatgatg ctaaattgaa aataagtaaa tttaatcaat
     2701 taggaataga agaagacttt aaaatagagg aggaataaaa ttggaattta aacaatgtct
     2761 aaaagaaaaa gcaagttttg tagaaaaagt acttaaagaa tatatgccta aagaagaagg
     2821 gtatcaaaaa actgttattg aagctatgaa ctatagttta agtgctggag gtaaaagatt
     2881 aagaccaata cttacattgg aggcttgtaa aatagttgga ggaaatgaag atgaagctat
     2941 tccttttgct attgcaattg aaatgataca tacatattca ctaatacatg atgatttgcc
     3001 agctcttgac aatgatgatt taagaagagg aagaccaaca aatcataaag tatatggaga
     3061 agctatgggg attttagcag gagatgcttt gttaaactat gcttttgaag taatgcttgc
     3121 aggctcaatc aataaagaaa atccagaaaa gtatctaaaa gctataaatg aaattgccaa
     3181 aggtgctggt atatatggaa tgataggtgg tcaagtagta gatgtagaaa gcgaaaataa
     3241 acaaattgaa aaagaaaaat tagattatat acatatgaat aaaactgctg caatgatggt
     3301 aggctgtatg agagcaggag ctactatagg tggagcaaat tctgaacaaa tggaagaaat
     3361 aactaaatat gcaaaaaata tcggattatc attccagata gtagatgata tactagacat
     3421 agtaggagat gaagctaagt taggtaaaaa ggttggaagt gatatagaaa atcataagtc
     3481 aacataccca tctcttttag gtttagataa atctaaagaa attgcacata atttgattga
     3541 tgaagcaaag aaaagtatag aaaaattatc tgatgatgta gattttttaa agggattggc
     3601 tgaatatata atagatagag aatactaaaa tatacagcta aaatcgagga gggaaaaatg
     3661 gatttttttt cggagatttt taacaatggg gctttgggaa taagtctgat tgcttgtttc
     3721 ttagcacaat ttataaaaat atttactggt aaggaaaaaa gaattgagtt atcaagaata
     3781 cttatttcag gaggtatgcc tagttcccat agttcttttg ttacaagttt agctacagtg
     3841 gtaggtattg aaaaagggtt taattctact gattttgcta taattaccgt acttgcctta
     3901 attataatgt atgatgcagc aggtgttaga cgagctgttg gtaaacaagc tactatatta
     3961 aatcaaatgg tggcagatat acagcatgga aagcatatag agcagaagaa attaaaagaa
     4021 ttaataggtc atacaccact agaagtttgg tttggagctt tacttggaat agtaacagct
     4081 ttaatattga tgtaaattga aaatttacag aataaaggtg aggataatat gtataaatat
     4141 ttagataaag taaattctcc aaaggatata aaaaatatga gtatagaaga aatggattta
     4201 cttgcaaagg atataagaaa gttcttagta aaatctgttt caaagacagg aggacactta
     4261 gcttctaatc taggagtggt tgagttaaca ttagcacttc ataaagtatt tgatagccct
     4321 aaagataaga ttgtatggga tgtagggcat caatcttatg ttcataaaat agttactgga
     4381 agaaaagatt gttttgtatc tttgagacag tttaatggac taagtggttt tccaaaggag
     4441 aatgaaagtc ctcatgatat ttttgataca ggacatagca gtacatctat ttctatagct
     4501 acaggtattg cctgcgctag ggatataaaa aaagaaaact atagtgttat atctgtgata
     4561 ggagatggtt ctataacagg aggtatggca cttgaagctt taaatcaatt aggatatata
     4621 gataccaata tgatagtaat ccttaatgat aatgagatgt ccatagataa aaatgttgga
     4681 ggaatgtcta aatatctgtc aagtattata agaaactcaa cagttgagaa aatgacagat
     4741 gaagttgata aaattttaaa tgttacacaa acaggtgaaa ttttatctaa gactgcacat
     4801 agatttaaag ataaactaat gtatagcttt tctcctcagg actgttcatt ttttgattca
     4861 ttaggtataa gatattatgg acctatagat ggccataata ctaaagaact aatagatata
     4921 ttgagaaaag ctaaacacaa aaaagggcct gttcttttac atgtaattac gaaaaaagga
     4981 aaagggtaca gatttgcaga agaacaacct gataaatatc atggagtatc aaagtttgat
     5041 ataaagacag gtgttacatc tgcaaaagtt aagtctatgt caattagtgt tggagaaaaa
     5101 cttgttgata tggctaataa taatgaagat atagtagcaa taacagctgc aatgccatct
     5161 ggtacagggt taaacctgtt tgaaagtgca tatccaaaaa gatattatga tgttggtata
     5221 gctgaacaac atgccacagg atttgcagca ggtcttgcaa aaaatggtat gaaaccttac
     5281 tttgctgtgt actcatcatt tttgcaaagg gcatatgacc aagttattca tgatgtatgc
     5341 ataacaaaaa agccagttac ttttcttata gatagagctg gattagttgg aaatgatgga
     5401 gaaactcatc atggtatgtt tgatttaagt tacttaaatt ctattccaaa tattgtagta
     5461 atggctccaa aagacacaag agagatggag cttatgatgg atttgtcatt aaaattagat
     5521 tgtccactag caattagata cccaagagga agtagttatt acctagataa gggagaatat
     5581 ggagagattg tactgggtaa atatgaggta ttagatgacg gtcaagatac agtcatactt
     5641 tgtattggaa gtatggtaaa acatgcttta gaggctaaag aaatattatc aagagaagga
     5701 ataaatccga caatagttaa tgctagattc ttaaagccaa ttgatgaagg tatgttaaaa
     5761 gcattattaa aaaatcataa aaatgtagtt actatagagg ataatattgt aactggagga
     5821 tttggaagta gaataaacaa gtttattata gataatgaat ataacgtaaa tatacttaat
     5881 atagcaatac cagaagaatt tgtaaaacat ggcaatatag atgaattata tgattttgta
     5941 ggattatcgc caaaaagtat tgctgataaa ataaggaaat tagttattga gtaaaaatct
     6001 aatgtagata ttgtaaatta aaatttatat aaaatataga atttatcaat tagaataaat
     6061 atcaagtgtg ttacttcaag ctactttagt tatcattaaa gtagctttta acagaaaggt
     6121 aattgtatga aaaaaagaat agatttatta ttagtagaac aagggtactt tgaaagtaga
     6181 gaaagagcca aaaaagcaat aatggctgga ttggtttttg ttgataatca aaggtgtgat
     6241 aaggctggta cagaagtaaa agaagattgt agtatagaag taaaaggaaa tccaatacca
     6301 tatgtcagta gaggtggttt aaagcttgaa aaagcaatga aaaactttga tttgactatt
     6361 gatggaaagg tatgtatgga tataggggca tccacaggtg gttttacaga ttgtatgctc
     6421 aaaaatggag caataaaagt attttctata gatgttggtt atggtcaact tgcatggaaa
     6481 ttgagacaag atgatagggt tgtgtgtatg gaaagaacta atataagaaa tgttacaata
     6541 gaatatacaa agcagtttgc tgattttgcg tcaattgatg tgtcttttat atcgttaaag
     6601 ttagtacttc caaaggcaaa ggagttagta cgtcatgatg gggaagttgt cgctttaatt
     6661 aaacctcaat ttgaagcagg aagagaaaaa gtaggtaaaa aaggagtagt tagagaaaaa
     6721 tctacacata tagaagttat aaaaatgatt tctgatttct cagtcgaaaa tggatttgag
     6781 attttagggt tagatttttc tcctattaaa ggtcctgaag gtaatataga atatctaatt
     6841 catttgagaa atggaaatga aggatatgaa tttgatggtg agacatacaa taacaaaata
     6901 gtagaagttg ttgaagcttc tcataattta gacaaatagg ttaaaagggg gcttagattt
     6961 gatcctcgaa ttatatatga aaaattgtgc cttagttgaa gaactaaggt tgaatattga
     7021 taaaaactta aatatactta caggtgaaac tggttcagga aaatctataa taatagatgc
     7081 acttggactt tgtttaggtg ataaatatga taggtctttt ttaagaaaag gaacagataa
     7141 gggacttgtt gaggctgtat ttttttcaga caatagatat cttaaaaaaa tattagaaga
     7201 aaatgatata agtatggaag atgataattt attggtaata actaggttga tatactcaga
     7261 tggtaagagt actgctagag taaatggaag gactgttaaa gtgtctcttc ttaaagaaat
     7321 tgcatctaca cttatagata tacatggtca acatcaaaat caagctttgt tcaataaaga
     7381 tactcattta aaatttctag acctttttgg agaaaatgaa ttggaagagt ttaaaattgc
     7441 ttataaaaat gtgtaccata agtatagtga agtaaaaaaa gctttaaatt gcttgacaga
     7501 aaataaagat gagatgcaaa ttcaacgaga aatagattta ttaagatttc aaataaatga
     7561 aattgaagct gcaaatctaa ataagaatga atatgaagat ttattaaaac aaagagaagt
     7621 ttatagaaat agtgaaaaga tatataataa tctaaattcg agttatagca agttacataa
     7681 tggtgagtat aatgttattg acctaatagg tcttgcatct aaggaactta atgacatatc
     7741 taaatatgac agtgttttaa gtgaatatag tgatactgta gaaagaatta tgtatgagtt
     7801 acaagacata tctggtgaga taagaaatta caaagataat atagactttg aaccttatga
     7861 gctagagcag attgagctta gaatcgacga aattaataat ttaagaagaa aatatggaga
     7921 cagtatagat gatatatttg agtattatgg aaaaattaaa gatagattag atgaaatatt
     7981 aaatagagat gaaagagttg aacagttaaa aagcaaactg atgaatatag aagaagattt
     8041 aaaaataaaa gccagtaaat tgactaaagc aagaatagaa gtagcaacta ctttagaaaa
     8101 aatacttctt gatgaattaa aaagcttaaa tatgaaaaat gtaatgttca aggttaattt
     8161 tgaaaaaggt tcatttactt tagatggagt agatgatatt gaatttatga tatcttttaa
     8221 cttaggagaa gatattaagc ctatatataa agttgcatca ggtggtgaga tgtccagatt
     8281 tatgttagcg tttaaaacca tattggctga tatagatgat attgatacat tagtgtttga
     8341 tgagatagat actggaataa gtgggatagc agcacagata gttggtgaga aattgagtga
     8401 tatagcaaag aaaaaacaga taatatgtat aactcattta cctcaaattg ctgcaaatgc
     8461 agacactcat tattgtatag agaaagatac ttcaaataat aggacgttta ccaatgttag
     8521 taagttaaat gaatctcaac gaaaaaatga aattgccaga cttatagcag gtaacaatat
     8581 aacagaaaaa actattgaac atgcaagtga aatcattgaa ttggctaaaa aatgttagtg
     8641 aaaaacaagc attttaatag gtgtaaagaa tttttataga aatgctttac acctattttt
     8701 tatgatgaaa ttattttaaa atatctagtg cctatattga agaaaagtag gtttgaaaat
     8761 ggaatgaact ttgtataaat ttgtaacttt gaaatttcta aatgcttaag tataatatta
     8821 tttattaagg tagtagtgag gggaggcata aaatgaataa aaaagatact atgcacttaa
     8881 tttttataat tgtaattttt attttattat ggtatattgt ggtagcagta agtgagatta
     8941 ataaaaatat tgaaagttgt agtaacaaat tagattattt gtcacaagag cttatgagta
     9001 ctaagtcgga tattagtaat actataaatg aagaaatgtc aaaaagttat ataacaaaaa
     9061 gtattgactt aaaagtaaaa aaaatcgaga aaaaagaatg tactattgat gtagatgttc
     9121 aattaagtaa acttggaaaa aatggaaaag tattttttat gtataaagaa gatagtgata
     9181 aatggaatga aactgagatg acaaaacatg gggaattatc atatgcatgt gaaataaaga
     9241 taaatacagg aagtgaatat gattataaag tagttacaag tggagatata tctgaaagtt
     9301 ctgatgtaca aaaactgact aaatctgatt atatgcctga acaacctata tttaattctg
     9361 ggataagaga taatagagaa tactttattg aaatagtaga aaatagtaat ctttttaatc
     9421 atgagagtga aaagtcaaaa aataaagctt acgataatat caggttagaa aaagtagata
     9481 ttattgtaaa tgaaggaaaa aaagacacta tatacaaagc ccaattagct gaaggcttag
     9541 atgatggcaa aactaataat tcaaatagaa atcaagtgaa ttatgaagta agttttccta
     9601 aaagggatat tgatgacata atttatatta aagctaaact tacatacaat aatggagtag
     9661 aatatacaaa ggatataact gaagatataa ctatgggatt acaagattta gaaaaataaa
     9721 tacatattta aataaatatt caaatagatt tgttcacaaa tagagtataa ataaaactgt
     9781 agtagtttta cttatactct attaaaaaat tataatttta attccatata tctagcccct
     9841 tcaattggat tataagtata aagttctata tcataaaaac caaacttttc atataatcct
     9901 tgtgcacttt tcatgcttgg aagagtatct aatctcatgt gtgtatatcc aattttttta
     9961 gcttctacaa tgatttcttc caatagtatt tttcctattt ttaatcctct aaattgattt
    10021 cttacatata atctttttaa ttcacatatt ttaccttcaa gtttttttaa agcaacacaa
    10081 cctgccaaat tttcatcaac aaatgccaat atcaatgagc ctgaaggttt tttatatttt
    10141 cctggaagtg tttttaattc attattaaag tcttgaaaac ataagtctat atttagagaa
    10201 tttgaatatt cagtaaatag aagttttacg ttttcaagat tttctaaatc tttttcttca
    10261 acaaattgta aagttatatt ttgagccatt ttatccgcca ttatataaac atgtttatat
    10321 aatgacttca cctccttgat atatgtatat ttttcttaat atcaattaga ttttaatacc
    10381 attacttttt caccaccata gtcaagctct ccattaaact taaatccaaa atctttataa
    10441 agcttaatag ccattttatt gtcttcgaat atactaagat atagttcatc acagttatat
    10501 tcatttttaa gatgatttac aagaaaatct aatgatgctt ttccacatcc tttaccttga
    10561 tattttgaat caatcataaa tctatcaagc caaactctat catcttctaa acgaccatac
    10621 atagcaaatc ctattaaaga attttcatca tatattccaa caggtaccca agctgttata
    10681 aatttagatt cagcaataga aagtgcattg ctttctatgt aatctaactg acttttatct
    10741 acagacaaac taactaaatc gtgccaatta tttatgtctg caattcttac atttaatttc
    10801 aagatattac cgtccttcaa actttttgtt acaatctaat actatcatat tatgtattaa
    10861 aaatcactta ttaagatttt aaataaactt tttgaatgaa taaattctca ggaaaaagga
    10921 attataaata cataataata cagcatagga gtagatggat atatgaacaa aataaataaa
    10981 aatttcaaaa atatttggat aatattagga gctgtatcga tttttaccat ggttataatt
    11041 tttaataaaa attgtaatgt taataaagag actcaaatag aagcttttac aattaagaat
    11101 catgataaaa aatttgtata tcctatggga aatataattg gagtaaaagc aaatacagat
    11161 ggtgtattgg tcttaggata tgaggaagag gatgtaggct atattggagg catacaaata
    11221 ggtgataata tagtaaaaat agataataaa aggataaaaa atagtcaaga tgtatcagag
    11281 attttgaata agattaaaaa aagtaaagtt gaagtgactt ttgagagaaa aaataagtat
    11341 aaaacagaag taatagagac taaaaaagaa aatggcaagt ataaacttgg attatgggta
    11401 agagataaaa tatctggaat tggaactatg actttttacg accctagtat ggaaaagttc
    11461 aaagcaatcg gtcatgccat aaaagattcg gatactaatg aactattaaa gataaagcaa
    11521 ggctatatat ataagccaga acaattaaaa atagtaaaag ctagtaatga aaaagtaggt
    11581 aaaataaaag gagattttaa tgacagcaat ttaatgggta atttctcaaa taattcagag
    11641 ctaggtataa gtggtaatat tacagaaaac cataataaag agtttaatgt agcaaataaa
    11701 gaaaagcaat taatagaagt tggaagaccg caagatgtaa aaataggaga tgcagtaatt
    11761 ctttttgagg ataaaaacaa aaatataaca agctatgata taaaaataga aagtatagta
    11821 tatgataaag gaaattatag agatatggta ataaaagtag tagatgacaa gttattggaa
    11881 tacacaggag gtatcgtaca ggggatgagt ggagctccaa taatacaaaa taataaaatt
    11941 attggtgcaa taactcatgt ttttagagat aatccgaaaa aaggatatgg tatttttata
    12001 gatgaaatga taaaattgta ggtgaggcat taaaaaattt tattatttta tcaattatct
    12061 aggaggaata taattttgga gtgtcgaata tgctttagag tagataatta ggaagcaatt
    12121 gtgtaaaaag tttagttttc tgtaataaga agatgttttt taatgggggg atttttagtg
    12181 gaaaaaatca aaatagtttt agcagatgac aataaggatt tttgtcaggt attaaaagag
    12241 tatttgtcta atgaagatga tatcgatata ttaggcatag ctaaggatgg aattgaagca
    12301 ttagacttag taaaaaagac acaaccggac ttattaatac tagatgtaat aatgccacat
    12361 ctagatggat taggtgtaat tgaaaaattg aatactatgg atatacctaa aatgccaaaa
    12421 ataatagtac tatcagcagt aggtcaagat aagataactc aaagcgcaat aaatctagga
    12481 gcagattact acatagtaaa gccatttgat tttgttgtgt ttataaacag aattagagag
    12541 ctagtttcta atagagttac acaagttgag ccaaaaccta gaccagttca agagactcaa
    12601 atgactagaa gtgattttgt taagaatgta ggaaatatag agacagaaat cacaaatata
    12661 atacatgaaa taggagtacc agctcatata aaaggatatt tatatttaag agaagcaata
    12721 aaaatggtta ttgataatgt tgagctttta ggtgcagtaa caaaagagtt atatccaagt
    12781 atagctaaaa aattcaatac aacaccaagt agagttgaaa gagcaataag acatgcaata
    12841 gaggttgcat ggagtagagg aaaagttgac acaataaatc aattatttgg atatacggta
    12901 cacaatacta aaggaaaacc aactaattca gaatttatag caatgattgc tgataaatta
    12961 agactagaac atagtatggt taaataaaca agacataaaa agtaaggctt tttaattaag
    13021 gcattggcta taaatgcgta ttacaagcag cgaaacggta taaccactag ggttataccg
    13081 tttcgctatt ttaataaata taaaaatttt ctttattatt tgcttactat atcaatataa
    13141 taattttatt atactatgga tatagtatgt gtctttacaa gttgtaaact gacagtggtt
    13201 tattttttaa tataaatatt gactttgatg caggtaaact ttgtattttt aagcgtatta
    13261 tggaatatgt taaataaaaa aatgatgaaa tatagtattg taaatgccaa agatgcaaaa
    13321 caaacttaaa acatttattt tattgttaag taatgctata atataatgtg attttaataa
    13381 tgatagtgga ggtttaaata tgagagtcga ggcccctata aaagtagatc gaaaaaccaa
    13441 aaaacttgct aaaagagttg aaagtgggga aatagcagtt ataaatcata tagacataga
    13501 tgaagttgct gcaaactctt tagtagaagc taaaataaaa cttgtcataa atgcggctcc
    13561 ttctataagt ggtaggtatc ccaataaagg tccagggata ttaactgaaa ataatatact
    13621 aataattgat aatgttggag aagagttatt tgaagaatta aaagaaggag aaactataga
    13681 agttgtagat ggaaagatat atagaaaagg aaaattttta ggtgcaggag aagttttaga
    13741 caagtatgag gtttcacacc aaataaaggc tgcctatgag aatttagctg tagaattgga
    13801 tagatttata gataatacta tagattatgc taaaaaagaa aaaggattta tacttggaga
    13861 agtagaaatt ccaaaagtga aaactaatta tgctaataaa catgtattga tagttgtaag
    13921 aggtcaggac tacaaagaag atttaagtac tatgctttct tatatagaag aggtaaagcc
    13981 agtattggtt ggtgttgatg gaggagcaga cgctttaatt gagtttggat atactccaga
    14041 tgtgattgta ggtgatatgg atagtgtaag tgatgaagct ttaaaaaagg ctagtgaaat
    14101 agtagtacat gcttatacag atggaagagc accaggtctt aagagggttg aggaattagg
    14161 cttagatgca gttgtatttc ctgcgccagg aactagtgaa gatatagcta tgcttatcgc
    14221 ctatgagtat aaggctgagt taatagtagc agttggtaca cattcaaata tgattgattt
    14281 tcttgaaaaa ggaagaaaag gtatggcaag tactttttta gttagactaa aaataggttc
    14341 aaagcttata gatgctaaag gcgttaattt gttatacaga agtaaattaa aaataaagta
    14401 catatgggca ttaatagcaa cagcattatt tcctgtgttg gtagttgcat cattgtcacc
    14461 aggagttcaa cagttcatac aactaatgca attaaagttt agagtattat tacagatgta
    14521 aattttagga ggataaatat gcatattaat atgaaatatt atatagtcac tataggggct
    14581 atatttatag ctttgggtat aggcatgtta gtaggattta atttaaataa taatcaacaa
    14641 ttaagtgaac aacaagcaaa cataataaat gatttggatg ataagtttaa tatcttaaaa
    14701 gaaaaaaatg ataaacttga cagtgactta gcaagtgtaa atagagatta tgaagaggca
    14761 gttaatttta taaataaaaa tgtagataag attttagctg gttcgctgaa tggtaaaagt
    14821 ataggaataa tatcggctaa tgaaaatgac gattatacaa aaaatataga agacataata
    14881 aataaatcta atggaagtat tgcatttaat atagtattaa aagaaaatat aacaaatcca
    14941 gaaaagttaa aggaaatgtc aactaaacta ggtacagatg taaaaaatgc aaatgatgca
    15001 gttaattata taattgacac ccttaaaaag gaagatgcaa gtgacatttt aacatatctt
    15061 caagaactag atgtaataaa gtttaactct cttggaaata catatctaaa atatgattct
    15121 gttgtaatag ctggtggaaa tgatgcaaaa gacagtacaa aacagtttga aaaaatagaa
    15181 aaatttgtag tttcaaaact taaatcagag aataaatatt tagtagaagt tcaaaataca
    15241 ggagttaaaa cttcttatgt agaactatat tctaaaaaca aagttgcaac tatagacaat
    15301 attgatgaag gtataggaag tatatctcta gctattcttc tacaacaagg taatatagtt
    15361 ggtaattttg gtagattgga tactgcaact tcattgttgc catctattaa atagaaataa
    15421 ttttaaggag agaaaaaatg gattcttata taagtataat aataccagct tataatgaag
    15481 aaagtagaat aaaatataca ttagaaagca tattggacat taagcaaata aatgaaataa
    15541 tagttgtgga tgatggttca attgacaata ctgctaaggt tgtatctgag ctagaaaatg
    15601 aaaagattaa attttttaag ttagataaaa atagaggtaa aggatatgct ttaaattatg
    15661 gtatcaaatt agcaatgaaa aatgcagata ttataggttt tctggatgga gatttaggaa
    15721 gctctgctaa agaagttgaa aaattaatag tacctatatt aaatggtgag gcggatgtta
    15781 caatagccaa gtttcctcct gcaaagagaa aaggaggttt gggatttgta aaaggtttag
    15841 ctaaaaattc tgtgtttgag atgactggtg ttgagttaga tgcaacttta tcaggtcaaa
    15901 gaatatttaa aagagaagtt ttagaaaagt ttgatgaaat accttttgga tatggagtag
    15961 aagttggaat gaccattgat atattaaagt atggatttaa aattaaagaa gtattagtaa
    16021 atatgactca taatgaaaca ggaagaaatt taaaaggttt tattcataga gggaaacagt
    16081 attatcacat aaaaaaggtt ttaggacaaa aaaagaaaga atggaggtaa tatcttagtg
    16141 aaagattata tactttatac cattttatta ttaactggat tatttggaac ctatgctgta
    16201 attccactat ttaaaaattt attgataaat ggaaatgtac ttagaccaaa ctataaaaaa
    16261 gatatgatac cagtaagtat gggaattgta tttttgccta tgattgtaat caatggaata
    16321 atacttgctt tctttactac agaatttaag gatttagcgt atatatttat gtttatgttt
    16381 ggtatgatat ccatgttttt tgcagggata ctagatgatg tcattggaaa tagagatgta
    16441 agtggtttaa aggggcattt taaaagttta tttaaaggaa gtcttacaac aggaggtttt
    16501 aaagctttat ttggtggatt tgtagggctt atagtttctg ttgctatatc aaaagacata
    16561 attgatataa tagtaaatac actaataata gctctatcaa ctaatttaat gaatcttttg
    16621 gatttaagac caggaagggc aataaaagca tatttattca taatggtaat tatattttta
    16681 acattgacag gatttgttca agttcttcca ttacttatag taccaaatgt acttgcatac
    16741 tttaattatg atttaaaagc aagggctatg atgggagata ctggttcaaa cgtccttgga
    16801 atatctattg gaatgctcat ttcttttggg tatccattta acattagatt aggatggtta
    16861 atatttgtag ttattatgca tatattcact gagaaatact cattaactaa gataatagaa
    16921 aaaaataaac tattaaattt tatagataaa ttgggaaggt agtattatga taagcaaaag
    16981 agtggggatt gttgaatcaa tagtaagtca aactgaaact ctagatgata taagagtaaa
    17041 tataaatgga aaaatacaaa gggcatataa ttatcccaaa atatctggaa ctataaatat
    17101 aggtgatgaa gtggttttaa atacaacagc agtagaactg agcttaggta caggtggata
    17161 ccattttgta ataactaatt taaataatat agaatctact ttaactgaag gtgggcatat
    17221 aatgaaactt agatatacac ctcttcaaat aaaagtggat tctgttgagg aacaagaaag
    17281 tatttatcat gataaatttg cgaattttaa aggattagaa ggtctttctg ttgtagtagg
    17341 gactcttcat agtatgttaa ctccatttgc agcttcattt aaaagaaata atccaaataa
    17401 aaaattagta tatataatga ctgatggagc atctcttcca atttatttaa gtaaaaatgt
    17461 ggacacttta aaagaaaaaa aattaataga tagtacgata actataggaa atgcgtttgg
    17521 tggagattat gaatgtataa atatatatac tgcattaatt acagcaaaag aaatactaaa
    17581 agcggatgca gtatttgtca gtatgggacc tggaattgct gggacaggaa caaaatatgg
    17641 atttacaggg attgaacaag gctctattct tgatgccgta aaaaaacttg aaggaagagc
    17701 aattgcaatt ccaagaatta gttttgctga caaacgagaa agacatcaag gtatttcaca
    17761 tcatagtatg actgtattta aagaaattgt aaatgtaaat gtagacatac caataactat
    17821 atatgatgat gaaaaattga actttataaa agagcaaatc aatttaaatg gtttagacaa
    17881 aaagcataat ataatttata tagataataa taaaacgaaa gaggatttag actattttaa
    17941 tctaaaagta aagagtatgg gaagaaatta tgaacaagat gaggcatttt tcaaagctgc
    18001 aagcacagca gcatattact taatggaggt ttaagatggt attagaagaa aaaactatta
    18061 gtagtgatag ggtttataca ggtaaagtaa ttactttaaa ggttgatact gttgaaattc
    18121 cagggcaagg atatcaaaaa agggagttag tagaagttgg tggagcagta ggaatagttg
    18181 caatcacaga tgataataaa gttgtactag ttaagcaatt tagaaaacct atagaaaagc
    18241 caatctttga aattccagct ggtaaactag aaaaaaatga aagtccaaaa gaatgtgctg
    18301 aaagagaatt aaaagaagaa actgggtata gtgcaaaaaa tattaagcta atacataaat
    18361 ttttcacttc agcaggtttt tctaatgaga taatgtttgt atatttagca acaggtctta
    18421 ctccaggaga gaataattta gatgcagatg agtttttaga tgtctatgaa attgagcttg
    18481 aagaagcata taacatggta ttaaaaaatg acgttgaaga tgcaaaaaca tctattggat
    18541 tattattagt aaaagatatg tttaaaaatt aatatttata aaatgaataa ccctttgtcc
    18601 taaagcatat tattttataa aggacaaggg ggaattaaat tgagaagaac atatagaaaa
    18661 aatgatttta gagaattaaa tagacaaata attattatag gattgttgtt tatgatgtct
    18721 attgttgttg ggtcttacat aaataaaata ttgccaggtt ctagtaacaa tatattaaac
    18781 aatataaatc ctgctgtaga gtattataat ctaaatataa gcataaaaga tacagtgata
    18841 caaaatttaa aatcagatgc aatctttatg ggctcaatag ctctattaag tctatttgta
    18901 gtaacaattc cagcagtgtt ggtagctttt gtattaaaag ggatgtcaat tggttataca
    18961 ataaatagtt gtatattggc attaaaattt aaaagtataa aaatgatttt aataatatta
    19021 tttaaaaatt tgattattat tccatgtgca atcatattag cattaattag tttaagttat
    19081 tttaaagaaa tggtttatga atttaaaaag aaaaatagaa aaaatatgca atttttaatt
    19141 aagaggtata tactaaatat tataattata atagcactat cattggggtt gcaattaata
    19201 ttaaatactg taagtattgg tattattaag ttcctagcta aataaaggag tttataatgg
    19261 atattataga gggatatata gattacataa aagataaaaa aaaattatca gagaacacag
    19321 tggcatcata ctttatggat ataaaaaaat atatggaata tataaatcaa aaaagcatta
    19381 aactagtaga tatagtagaa aatgatatta taagttactt aataaagttg gaaaaagata
    19441 atgtatcaat agctacaata gcgaggatga tatcctctat aaaatctttt catgattatt
    19501 tatttttaaa tcatatatgt acaaataatc ctgcaaagga tataaaaaaa cctaaaataa
    19561 aaaaagaaaa tataaatata cttacagaag aagaaattga aagactatta aattttccaa
    19621 agttaacaac acctaaattg attagggaca aggctatttt tgaagtatta tatggtactg
    19681 gtataaaagt atctgaatta gtagagatga atatagagga tattgaccta gatattgatt
    19741 atatatattg taattcatgt tgtaaaaatc aaagagtgat accactttgt gatattacaa
    19801 aactttactt agaaaagtat ctaaaagaag caagacctaa aatggctgta gaaggtgaaa
    19861 aatcattatt cgtaagttca ttaggtcaaa ggtttacacg acaaggtctt tggaaagtca
    19921 taaaaaaata ttctaatcta gccaatatag ataaaaatat aaatcctaca atgcttagac
    19981 attcatttgc tattcatttg ttaaatgaag gtgctaacat tgctgttgta agtaaaattt
    20041 taggaaatgt taatttatct agtttgcaag tatatttaaa tcatatagat aaaaacgtga
    20101 gaagagaaat aaaggaaaaa catcctagaa atgatgttga tgtagaatta caaaaagcag
    20161 aagctaaata gactatatag caaattttat aatttaaaag gagtatattt atatgagcag
    20221 agtaatttgg attgtaattg atagtgttgg gataggagca ttacctgatg ctgaaaaatt
    20281 tggtgatagt aaggatgtaa gtacccttgg aaatatattt aaggaatatc cagatattca
    20341 gattcctaat atgagaaatt taggtatagg aaacatagat ggtatagatt tttttgaaag
    20401 tataaaagaa ccaataggtt gttttggaaa gtgtaaggaa atgtctcagg gaaaagatac
    20461 tactacaggt cattgggaaa tgactggtat aatagttgac aaaccattta agacatttga
    20521 acatggattc tctaaggaga taatagaaga gtttgagaaa aaaactggaa gaaaagtagt
    20581 aggaaataaa ccagcttcag gaactgtaat aatagatgag tatggagagc atcaaataaa
    20641 aactggagat gtaatagtat atacatcagc tgatagtgta tttcagattg ctgctaatga
    20701 agaagtaatt cccctagaag aactctataa tatgtgcaaa atcgctaggg aaataatgat
    20761 gggggataat gcagtagcta gagtaattgc tagaccattc ataggtaaga aaaaaggtga
    20821 gtttgtgagg acttcaaata gaagagatta ttcactagac ccatttgaac caacagttct
    20881 tgataatata aaagaatcag gacttgatgt tttagcagtt ggaaaaatag aagatatttt
    20941 taatggaaaa ggaattacag atgctatcca cactaaaagc aatatggatg gagtggatga
    21001 aactttaaat tatatgaaac aagataataa aggtctaata tattcaaatc tcgttgattt
    21061 tgattctaaa tatggtcata gaagagaccc agaaggatat aagaaagctc ttgaagagtt
    21121 tgatagtaga cttcctgaaa taatggctaa tatgagagaa gatgatattt taataatcaa
    21181 tgcagaccat ggaaatgacc caacatataa aggtacagac catacaagag aatatatacc
    21241 tgttatgatt tatggcaata agattaaaaa aggttttaat ttaggagtaa aagatacttt
    21301 tgctgatatt ggagcaactg ttgcagatat actaaatgta aagttgccta aacatgggtc
    21361 aagctttaaa gaagatttat tttaaactat aaaatgataa aaacttagct taataggatg
    21421 gagagattgg aatgtttgaa aagatagcac aaagtagtaa atttataaat tctaaaagta
    21481 atataaaacc taagattggt ttgatattag gttctggact aggtgattta gcaaatgata
    21541 tagaagaccc tgtcacaatt aaatatagtg aaattccaaa cttccctgta tcaacagtag
    21601 caggacatgc tggacaactt gtaattggaa aacttgaagg aaaagaagtt attgctatgc
    21661 aaggtagatt tcattactat gaaggatatt ctcagaaaga agctactttt ccagtaaggg
    21721 ttatgaaaga attaggagtc gagattctta tagtaactaa cgctgctgga ggcgtaaata
    21781 aagaatttaa agcaggagat ttgatgctta taagagacca tataaacttt agtggaagta
    21841 atccattagt aggaaaaaat gatgacagat ttggtgctag atttccagat atgtcagatg
    21901 catattcttc taaatatgta gatgtagtta aaaactgcgc taaagaatgt aatatagatg
    21961 tcaaagaagg cgtttatatg ttttttagtg gtccaaacta tgaaacacca gcagaagtga
    22021 gaatggcaca aattttggga gctgatgctg taggtatgtc tactgttcca gaagtcatag
    22081 tagcttctca ctccaatata ggagttattg gtatatcttg tataacaaat atggctgctg
    22141 gcatcttaaa tcaaccacta agtcatgagg aagtaataga aactactcaa aaagttaaat
    22201 cagaatttat gaatttagtt aaaacaacgg ttaaaaattt ataagttaaa gcttattata
    22261 tattttttga ggaagtttat aataaaatag ttaatgttta atttgaaaag aattaataaa
    22321 aagctgtctt gatatagaaa gttatttcta ataaaggaca gtttttttat aatactacaa
    22381 catttgaaag tgttttaaag tttaatattt atttaaatta ttatagatta gaatggagat
    22441 ttcaattatg agaatgtatg atattataaa aaaaaagaga gataacttag agcttagtaa
    22501 aaaagagata aactacttta tagaaaacta ttcaaaggga gatatacctg actatcaagc
    22561 ttctgcacta ttaatgtcga tttatttaaa taagatgaat aaacaggaaa ctgtacatct
    22621 tacagaagca atgatgaact ctggagacat gataaacctg tctgaggtag atggaataaa
    22681 ggtagataag catagtactg gtggtgttgg agataagact accatagctc ttataccttt
    22741 ggttgcttca tgtggagcac cagttgcaaa gatgtcaggt agaggtcttg gtcatactgg
    22801 tggtactatt gataaattgg agtctatacc aggtttttcg acagaaatgg agataagtaa
    22861 atttattgat tctgtaaata agtctaaaat agcagtttgt ggtcaaagtg caaaggtagc
    22921 aattgcagat aagaaaatat atgctcttag agatgttaca gcaactgttg ataatatatc
    22981 attgatatca tcaagtgtaa tgtctaaaaa gttagcttca ggagctgatg caattgtgtt
    23041 agatgtaaaa actggtgatg gagcatttat gaagacatta gaagagtctt ttgagttagc
    23101 taaatctatg gttgatatag gtactggtat gaatagagat acaataggga ttgtaacaga
    23161 tatggatgaa cctttaggtt ttgcagtggg aaactcttta gaagttatag aagcaattga
    23221 gacattaaaa ggaaatggcc ctaaagacct tgttgagcta tgtgaagtat taggagcata
    23281 tatgttggtt ttatcaaagg tagcacatga ttttgaagat ggtgttgaaa agataagaga
    23341 atcaataaaa tctggtactg ctatacaaaa actcaaagag tttatagaga atcaaggtgg
    23401 taataaagat gtagtagatg attattcttt attcaaaaaa tcaaagtata aatatccagt
    23461 taaatcaatt aaatcaggtt atataagtaa aattaaagct gaagatgttg gtgtatcagc
    23521 tatgattctt ggagcaggta gagaaactaa agatgatatt ttagatttat cagcagggat
    23581 aattttagaa aagaaagtag gagattatgt aaatgaaggt gaagttttag cttacatgta
    23641 ttataatgat gaacaaaagc tttcattggc aaagggaaga tttattgatt cctactctat
    23701 tgtagattca aaaatagaaa aaaataaatt aatatatggt attgtaacta aagaagagat
    23761 taaaaagttt tagaaaattt tagaaaagtg attataaaag gcctaacatg tcaaaaataa
    23821 tgttaggttt tttatttttt gataaactat aaattatacc aaaaatatat atttt
//
LOCUS       DAEPOG010000044        23732 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEPOG010000044 DAEPOG010000000
VERSION     DAEPOG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.044"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..308
                     /locus_tag="KR432_001479"
     CDS             <1..308
                     /locus_tag="KR432_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0640023.1"
                     /translation="WEIRFALVMFLIYYVDDKHIKEIFEYCNNIQSEEYYVKMGMAWL
                     LSICFIKCEEETFLYIKNNNLDDFTYNKMLQKIIESNRVDLEKKNIIRSMKRKTRRC"
     gene            322..996
                     /locus_tag="KR432_001480"
     CDS             322..996
                     /locus_tag="KR432_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0640024.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            1022..1858
                     /locus_tag="KR432_001481"
     CDS             1022..1858
                     /locus_tag="KR432_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640025.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1874..2545
                     /locus_tag="KR432_001482"
     CDS             1874..2545
                     /locus_tag="KR432_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640026.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2535..3767
                     /locus_tag="KR432_001483"
     CDS             2535..3767
                     /locus_tag="KR432_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640027.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3917..4234
                     /gene="trxA"
                     /locus_tag="KR432_001484"
     CDS             3917..4234
                     /gene="trxA"
                     /locus_tag="KR432_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0640028.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4251..5129
                     /locus_tag="KR432_001485"
     CDS             4251..5129
                     /locus_tag="KR432_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0640029.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            5399..5767
                     /locus_tag="KR432_001486"
     CDS             5399..5767
                     /locus_tag="KR432_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0640030.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            5770..6141
                     /locus_tag="KR432_001487"
     CDS             5770..6141
                     /locus_tag="KR432_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBH0640031.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(6285..6740)
                     /locus_tag="KR432_001488"
     CDS             complement(6285..6740)
                     /locus_tag="KR432_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640032.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(6779..8044)
                     /locus_tag="KR432_001489"
     CDS             complement(6779..8044)
                     /locus_tag="KR432_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBH0640033.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            8514..9965
                     /locus_tag="KR432_001490"
     CDS             8514..9965
                     /locus_tag="KR432_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0640034.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(10185..10646)
                     /locus_tag="KR432_001491"
     CDS             complement(10185..10646)
                     /locus_tag="KR432_001491"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH0640035.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(10752..11945)
                     /locus_tag="KR432_001492"
     CDS             complement(10752..11945)
                     /locus_tag="KR432_001492"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBH0640036.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(11968..12633)
                     /locus_tag="KR432_001493"
     CDS             complement(11968..12633)
                     /locus_tag="KR432_001493"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH0640037.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="KR432_001494"
     CDS             complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="KR432_001494"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH0640038.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(13902..14023)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14212..16695
                     /gene="recQ"
                     /locus_tag="KR432_001495"
     CDS             14212..16695
                     /gene="recQ"
                     /locus_tag="KR432_001495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBH0640039.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      16783..16894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17036..18337
                     /gene="thiC"
                     /locus_tag="KR432_001496"
     CDS             17036..18337
                     /gene="thiC"
                     /locus_tag="KR432_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0640040.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            18391..18585
                     /gene="thiS"
                     /locus_tag="KR432_001497"
     CDS             18391..18585
                     /gene="thiS"
                     /locus_tag="KR432_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH0640041.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            18578..19381
                     /gene="thiF"
                     /locus_tag="KR432_001498"
     CDS             18578..19381
                     /gene="thiF"
                     /locus_tag="KR432_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBH0640042.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            19444..20214
                     /locus_tag="KR432_001499"
     CDS             19444..20214
                     /locus_tag="KR432_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH0640043.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            20231..21334
                     /gene="thiH"
                     /locus_tag="KR432_001500"
     CDS             20231..21334
                     /gene="thiH"
                     /locus_tag="KR432_001500"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH0640044.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            21335..22024
                     /locus_tag="KR432_001501"
     CDS             21335..22024
                     /locus_tag="KR432_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0640045.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            22212..23606
                     /gene="yfcC"
                     /locus_tag="KR432_001502"
     CDS             22212..23606
                     /gene="yfcC"
                     /locus_tag="KR432_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH0640046.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         9115 a         2712 c         3946 g         7959 t
ORIGIN      
        1 catgggaaat tagatttgca ttagttatgt ttttaatata ttatgttgat gataaacata
       61 ttaaagaaat atttgaatac tgtaataata tacaaagtga agaatattat gttaaaatgg
      121 gtatggcatg gttgctatca atttgtttta ttaaatgtga agaggaaact tttctctata
      181 taaaaaataa taatttagat gatttcactt ataataaaat gctacaaaaa ataattgaat
      241 caaatagagt agatttagaa aagaagaata taataagaag tatgaaaaga aagacaagaa
      301 gatgttgacg gaggaatatt aatgaataac aatgaagaca gaattgacaa atttttagaa
      361 cttcaaagca aaggatttaa atttgcagag atagctcaaa atctaaagat tagtcaaagt
      421 actttaagaa cttttttaaa taaaaaagga tataagtcag aaaaaggtaa atacatatta
      481 aaagacgaat taaattataa taaaagtttg gataatagaa acttggaaga agagaaagct
      541 aaggagtatg aaaaaagtaa aaagaatact ataagtaata gaaaaaaagt aagcaccaaa
      601 aaaaagattt ctaatgaaaa ggctttaagt aaaagtgttg aacaaattga gtttaatgat
      661 acaggtttaa aaaatgcaaa gcgaaaacaa aatgttaagc ctaagaaaga tagaaaaata
      721 aatataactc aagaagattt agataagctt tgtgaagtat atgattggta tttagaagta
      781 aaagattaca atataaagcc taaaaaagta tctaataaaa aagatgtaca tatagatgat
      841 gaacacatag atgaattaaa aacaacaaga atacgtatag ataaagaaat atggaatgat
      901 tttgaaagat tatgtagtaa ttcgaatttt gaaaaacatc aaatacttac acaggcattg
      961 aaagaattta tgaaaaagta taaaaatcta ctataaaata ctactatatg aggtgcttaa
     1021 tatgaataaa atagtaaaaa aatcaatttc tctaatgatt atattaacaa tatttatttt
     1081 tatgttgaca gcatgtgaaa aagatgaaca gcctgattca gttgatattc aaacagaaga
     1141 taaaaatgag ataaaaatag atgagggaaa tgcaaaagta ttgaatatag gaaaatcaga
     1201 aattataaat atagatgaag gtgataaagt agatacatca acaaaggtag aaaataattc
     1261 aacatttaat atatccaatg ttgaattgat atataatgaa tatgatgaga ataaaaaaat
     1321 tatatcttcg gatagtaagg ctttgttgga tatgacttta atgcctggga aagttgctta
     1381 catagaatgt gggcataaaa cttttgtaaa gggtgtagaa gtttatgcat atgaatatga
     1441 ggcagagggt aagattgtgt atgttaattt aaaagaaaat actattaata taagaaataa
     1501 taatattaag ctagaaaata gtagtcagta tgaggtgcta tctacatcag aacttaaaaa
     1561 agtaaatgaa tcaaatgagg gaattacgta tcaagtaaaa gtaaaaaatt catcatcaaa
     1621 agatttagga aatattattc ttaagacagc agaagttaat gaaaatggag aatatttaac
     1681 agtaaatcgt gtaccatcat ataagatttt aaagccttca gaagagacag atatagatat
     1741 attgtgttca acaaaggcaa aaagtgttga aatagtggga tatacgtatg atgatataaa
     1801 agaaaaagcc aatgtggata ttgacttaaa atctcataaa gttaagatag acaaataaag
     1861 gtaggggata cttatgaagg ttttaatagt tgaagataat aagatacttt tagaaagtgt
     1921 agtagaagaa cttagcaagc attttgaaac tgaaaagtgt gaagatggag aagaagcttt
     1981 atacttagta aatcaaaata tatatgattt agttatttta gacttaatgc ttcctaatat
     2041 aaatggattt gatatcctaa agaaaatgag agttaataat atagatacac ctgtacttat
     2101 attgacagca aaagaaacat tagatgacaa agtagaagca tttactatag gagctaatga
     2161 ttatttgaca aaaccttttt atatggaaga gttagtagct agggtatacg ctatattaag
     2221 gactaatgga aagataaaag aaagaaacgg attagaattt aaatctttat atttagatac
     2281 actagaaaaa agagtgtata ttgaaaaaga agaaataaaa cttcaaaaca aacagtttaa
     2341 cttgcttgag tattttgtat taaataaagg ttcaatttta ttaaaagagc agatttatga
     2401 tagaatatgg ggaattgatt cagatgcaac tattgaaata gtggaagtat atgtaagtaa
     2461 tctaagaaaa aagctaagta aatatggata tgataaatat attaaaacta aacgtaaggt
     2521 aggatatata ttcgatgata aataagaatg tatttaccag tactaaaaat catttaataa
     2581 aaatgtatat tattgtagta ggtagttttt taataatctt ttctatattt atatactcat
     2641 attttagggg acttacatat agtggaattg atagtgagat aaatgatgaa cttgaatata
     2701 tagtctctca atttaaaaga acttcatttt tgaacccaat aagactgaaa gatcctaaag
     2761 atatggtgta tgtatatgaa gatggaagga taagttacta tactcaaaat gaatattttg
     2821 atgagttact tccagataga agactagata aaaaaaattc gttttttaaa tacacagaaa
     2881 atggatatac ttttagagaa ttaaatgtag atgtaggtag ataccaaata cagataatta
     2941 gaaatataga ttctgagatg aattctttaa gacagcttac atctgttctt ataattggaa
     3001 tattaatttc tgtaataatt acttactttg tggctgtata tctcactaga aaagctttaa
     3061 ttccaataga aacagcatgg aaaaatcaag ctaagtttat acaagatgct tctcatgaat
     3121 taagaacacc aataacaata gtttcatcaa aattagaaag tatgttaaaa tccccagaaa
     3181 gtacagtaaa tgatgaagtt gaaacaatag ctactgcaat gaaggaaact agaagactta
     3241 aaaaaatgat aactgattta ttgtctctta ctaaagagga ctctattgtt aaagttaatt
     3301 tagaagaaat agacttagaa aaattattgg aagaaatatc agaagactat attgacattg
     3361 ctgagtttca ggaaaaaaga ttcgtgttta attctaaact gaagaataaa gttataataa
     3421 cagataaaaa taaattaaga cagcttatat taattttcat tgataatgcc tttaaatata
     3481 caaaattggg tgatgagata gcattagaat taaaagaaga tatagaggat gaggtaactt
     3541 tacttataag tgacactggt ataggcataa aaaaagaaga gatacctctt atatttgata
     3601 gattttttag aagtgaaaat gtaagaaata aagatttaga aggttctgga ataggacttt
     3661 caatagctcg catgattagt ttaaacttat ctattgatat aaatgtaact tctgatgtag
     3721 atattggaac tacatttgaa ttgtcaatac caaaaaagtt aaaataataa aaatataaaa
     3781 aattgttttt agaaatgaag aatgggtata ctaattagta taaaatataa aaagacctta
     3841 taaaagtaaa tttatattta taaatttcat agtataatta tatagggata ataaagttta
     3901 ataggaggat ttaaaaatgg caaaagttat aaatacatct gaatttagaa gtagtgttga
     3961 aggtagtaag ggagtagttg tagttgattt ctttgcaact tggtgtggac cttgtaacat
     4021 gttgggacct gtatttgcag aattaggtga agagatgaag gataaagcta gatttgtaaa
     4081 agttgatata gatgagagct tagaaatagc tcaacaattc aatgtatcta cagtaccaac
     4141 tatgattata tttaaagatg gaaaaccagt agaaacttta attggattca tgccaaagaa
     4201 caaaattgaa atgcaagtaa aatcatattt ataaaaaatg ggtgagtatt atgagatatg
     4261 atatagctat aatcggcagt ggtccagctg gattgtctgc tgctataaat gcaaaaataa
     4321 gaaataaaac aattatcatg tttggtaatg ataatttaag taataagctt gttaaagctc
     4381 cttcaataga taattattta ggtttttatg atataagtgg tgatgaatta aaagacaagt
     4441 ttaaatcaca tatagatagt atggatattt caattgaaaa taaaagaata aataatattt
     4501 atgctatggg agaatacttt acaataatgt ctggaaatga tatgtatgaa gctactactg
     4561 taatccttgc aactggggta gagtatacta gaccaatcaa gggtgaagaa gaatttctag
     4621 gaagaggtgt aggctactgt gctacatgtg atgcaccact ttatagaaat aaaaaggtag
     4681 cagttatagg atataatgaa gaatctaaag aagaagcaaa ttttttaagt gaactcactt
     4741 caaagactta ttttatacct atgtacaaaa aagataatct tatgagaagt tcagataacc
     4801 tagatgattc tattgaagtt atacatgaca gaccagtaca gatagatgga gataaacttg
     4861 taaacaaagt atcttttaaa gaaaaccaca ttgaagttga tggagtattc gttataaaag
     4921 atagtacggc tccaagtgca ttggttcctg gaatagagat tgatggcatt catattaaag
     4981 ttgacaataa tatgaagact agtatagatg gatgctttgc tgctggagat tgtgttggaa
     5041 aaccctattc ttatataaaa gcagctggtc aagggcaaat agcagcttta aatgctgttt
     5101 actatttaga taaattaaag agggcttaat atttatattg attaactttt taaagatatt
     5161 tataaaaaac tgtcttgaaa taattttatt atttcaagac agttttttat ttttaagtta
     5221 ttaataaaag tattttaaaa tctatatgta attactttta ttaataaaat tcaaagttat
     5281 attagtagaa attggaatta tttataatat actccaaaat ttaaaaataa tggttgaaaa
     5341 atctaattaa gagtactata atttaattaa gtaattactt aatcaaaacg aggtacttat
     5401 ggaaaacttg gttaaaatat ttaaagcatt gagtgatgaa actagactta acatacttat
     5461 attagtatcc aagagaaata tatgtcaaaa ggggatttct aaatatctag ggatttcaga
     5521 ttctgctgtt tcacaacata taaaaatatt aaaagatgtt gggataataa caggatataa
     5581 agaaggatac tatgttttat atcatattaa taaagaatca tttaatatat gtgtagattt
     5641 tataaattct atgttaagta attctagtga aagctttata gatgtatttg atgtaaatac
     5701 aatacatcta ggatgcagta aagaatgtaa atctataaaa aaatgttgta agagaaggga
     5761 gaaataataa tgaaagtatg tataccagta gaggagaata aaggattgga tagtaaacca
     5821 tatggacatt ttggttctgc tccaattttt gttgtgtgtg atttagaaag tggagaagtt
     5881 aaatctttag ataatggaga tttagatcat gaacatggaa aatgtcaacc attaaaagca
     5941 ttgtcaggga cagctgtaga tgcagtagtt gttggaggaa taggacaagg agctataatt
     6001 aaattaaatg gaatgggaat aaaagtttac agagcagaag gtgatactat aagtgctaat
     6061 ttagacttac ttaaaaatgg aaaattagta gagtttccat cagaccatac ttgctcacat
     6121 gacggatgtg gacatcatta aaaaaaacag ctgatgaaat atatacttca tcagctattt
     6181 tttcttttaa taaatacgat tttattcttt taaaaccaaa atttagattt ttatgttttt
     6241 tgtagtatcc ttaataatat ttcaagttta caagtttact acttttagat tttttttgta
     6301 tgtttttcta tatagtcaat atcatttaat atgtcattga gttctttatt aagtgttttt
     6361 aaatcatcac catctacatt gtagtttaaa aatagttcct ctacattatc aatgatagaa
     6421 ttgacttttc tagtaacttc aacaaatgtc ttaaagttac tactattttt agcatttaga
     6481 aattttttta aatcctttga tataaatgat actatttcat attcatcatt aaatagacct
     6541 ctaccaatag gatatggttc aattctctgt aaaaaatatt ggtctttagc attaatatta
     6601 taagtataag atatttccca accgtctcct aaatcaaact tgataaaata taatgtacta
     6661 cttacttttg atattcttgt ggcaccaagc tgtcttaaaa gaacttcaat cgaatcacct
     6721 tgaacagtat taatattcat atatacacat cccctttagt attattatac tatatttttt
     6781 aaaattttat aattaaataa acagtagata taataataga tataatcata agtggaaaac
     6841 ctattttgaa atactcctta aaagagatag gatgaccgtg tttattacct atacctgcta
     6901 gtacaacatt agcagatgct cctattagtg ttccatttcc tccaagacaa gctccaagag
     6961 atgttgccca ccatagtggc attacatcaa taccttcagc ttgcatagtc aaaataagag
     7021 gaattagtgt tgcaacaaat ggaatattat ctaagaaaga agagactata gctgataacc
     7081 ataatatcaa caacattgta aatacaagat gtccttctgt aagtccaatc aatgcttgag
     7141 ccaatttgtt tattatccca acttctacta gtccaccaac tactacaaat aatcccataa
     7201 agaataaaat tgtggaccat tcaatacctg ccataatttc atctacatct tgttttccta
     7261 ttaaaagcat tatagatgct cctgtcagtg ctacaacaga agaatctatg tggattgtac
     7321 tgtgaaacat aaaaccaaaa agtatgaatg ctattaatat aaggcttttt ataagtagag
     7381 gtttatcttt aacagatttt ttttcatcta gttttaatat tgcattttta gctctttcat
     7441 tcacaacaag ttcttttcca taaataaatc taaagcatat tatagttata gcaagtatta
     7501 ctataacagc aggacctaaa tttattacaa aatccataaa actaagattg gcagcactac
     7561 caatcataat atttggaggg tctcctataa gagtagctgt tcctcctata tttgaagcta
     7621 aaatctgtgt tattaaaaat ggaactggat taagtcctaa tatttgtgta ataactatag
     7681 tcattggtcc aataagtaag acggttgtaa cattatctaa tacagctgat aaaatagctg
     7741 ttataatagc aaaacaaatc attatcttcc atgggtcacc tttagctttc tttgcagtcc
     7801 agatagctat atattcaaat aaccctgagt ttttgacaac agctacaaat agcatcatac
     7861 caactaatac accaatagta ttgaaatcta tatggcttaa tccttcatca agtgtaagta
     7921 tattgaatat tatcattaat agtgcaccac caacagctgc tacggatctg tttacctttt
     7981 ctgtaataat agctgccata actattaaaa atatacatat agctattatt tgttgaaatt
     8041 ccataatata agtacctcca tttgtattat tttgtgccta atgctagtac tatatatagt
     8101 attacttttt tattgttttt tcaatggatg tatgaaagta tattatttaa caaagttttt
     8161 gagaaattga cagaatttaa agaaaatttt tgttatttaa ataaatttta aaattattcc
     8221 attgataata atattaaacg acaaacgaaa aaatataccc tttaaactag aatttattgt
     8281 atccaataac aattataaaa tattaaatat atctatatta taaaaataat aaaaaaaggt
     8341 ttgatttgaa tataaaactg caatatatta aaaaaattat tcattttaaa ccttgtaatt
     8401 tgattttata aatattataa ttaaattaat gaatttttaa caaggaatta ttcatttttt
     8461 tgattttaag atttattgag aaaacaaaac taaatagggg ggtaattaat ataatgaatt
     8521 ttttaaatgg tattttaacg agtattaaca atgcagtagt atttgtaaac aattacttat
     8581 ggtcgtatat cttaattata atgttagttg taattggtat ttactttact attaaaacaa
     8641 attttgtaca attcagatat tttatagaaa tgtttagact tctgggtgat ggtacagcga
     8701 ataaaaatgc aaaaaaggaa ggaaaaatct cttcatttca agcattttgt ataagtacat
     8761 cttcaagagt tggaactgga aatatagctg gaatagcaat tgcagtggta gcagggggac
     8821 ctggtgctgt attttggatg tggcttatag cattaattgg ttctgcatca agctttgttg
     8881 agagtacatt agctcagatt tacaaagtga aagatggtca agcttttaga ggtggaccag
     8941 cgtattatat ggaacaaggt ctaaataaaa aatggatggg tattttattc tcaatactta
     9001 taactatatg ttatggtttt gtatttaatg cagttcaagc aaatactgtt tcacttgcat
     9061 ttaataatgc atttggaata agtaaaatgg ctatgggaat aggtcttgga attttaactg
     9121 caatagttat ttttggtgga gtgcatagag ttgcaaaagt ttctgaaata atagttcctg
     9181 tatttgcagg attatatata ttagttgctt tggctatagt tgtaatgaat ataacagaga
     9241 taccaagtgt tatagcattg atagttgaaa gtgcatttga ttttaaagga atggcgattg
     9301 gtacatttat gggagttgta atgactggag tgaagagagg tctattttct aatgaagcag
     9361 gtatgggtag tgctcctaat gctgctgcaa cagcacatgt aactcatcct gtaaaacaag
     9421 gtttaattca atctttaggt gtttttacag atactataat aatatgtagt tgtacagcat
     9481 tcatggtatt attatattca ggttatagta gtagtggagc aactggaatt gaattagctc
     9541 aagaagcttt aactcatcat ataggaccag ttggaaatat atttatagca gtttgtatat
     9601 tcttgtttgc atttagttct atagttggta attactatta tggtgaatca aatatggagt
     9661 ttatgagtgg aagtaagact aaacttaatg tatttagagt ttttgttgta ggcatggttt
     9721 tatttggttc acttactcaa gtagatgtag tttggaatct tgcagactta tttatggcat
     9781 tgatggcgat aataaactta atagcaatag cacttttagg aaaatatgca tttattgctt
     9841 taaaagatta tacaagtcaa aaaaaatcag gaataaaaga ccctgtgttt attgcagatg
     9901 aaatagaagg cctagaaaag gtatcagagt ggcataggga attatctgag gaacatttag
     9961 gataaaaaat agtataaaat tataaaacca ttttgaaaga ctagagtatg tatatgcttt
    10021 atatgaatat aatattgaat taatttatat tactggaatt tttaattcca gtaatatttt
    10081 tttattatat aatttagctt tattaaaagt ttttattatt aaaaatagct tatttatcgt
    10141 aaagtgaatg atattggttt tttaattatt atgaatgttg gtatttaatt taaagactta
    10201 aataaatttg ccatttctag cgcattcata gcacaatcat aacctttatt tccagctttt
    10261 gtacctgctc tttcaatagc ttgttcaata ttttctgtag ttacgattcc aaatataaca
    10321 ggtaattcac tgtctaaaga tacttttgct atccctttag agacttcact acatacataa
    10381 tcataatgag aagtagcccc tcttataaca cagcctaaac agattatagc atcataccta
    10441 ccacttttag ccattttttg agcaacaaga ggaatctcaa aagctccagg tacccaaact
    10501 atttctatat tttctggaga aacgtcatgt cttaaaagac agtcttcagc tcctgataaa
    10561 agttttgatg ttataaattc attaaaacga gaatttatta tacctatttt taaatctttt
    10621 ccaataagtt ttccttcata tatcattttt atttcctcca attttattta tgttatagat
    10681 ttatatttat aaatatcttt aattttaatt ataaaaaact acttagtaga aaaatatcat
    10741 catataatca tttatataat atctaaaaga tgtcccattt tatctttttt agtttttaaa
    10801 tatagcttat taaagtcatt aatttcttca ttaataggga ttctattttc gatttttatt
    10861 ccatatttct ctaattgatt tattttatcc gggttattac ttaaaagatt tatacttttt
    10921 atgtttaaat tttttaatat ttgagcagcc atatagaaat ctctcatatc ttcttcaaat
    10981 ccaagcatta aatttgcttc aacagtatcg taaccttctt cttgaagttt ataagcctta
    11041 attttattta aaaggccaat acctctacct tcttgcttca tataaattaa aactccactt
    11101 ccattttcca caattttctt catagaagat tctagctgta atccacaatc acattttaga
    11161 gagtggaaca catctcctgt taaacattca gaatgaagtc taactagagt attttctata
    11221 tttatatttc cataagtaag ggcaatgtgt tcttcatttg aataagtatc tctatatcct
    11281 atgatttcaa aatttccata ttttgttgga aggaaagcac tagatacttt ttctatagta
    11341 gtttcattta ttttgcggta ttctataagg tcttttatag tgatgatttt taaattatgc
    11401 ttttctttga aaatcattaa atcatcaact cttgccattg taccatcatc tttcattatt
    11461 tcacagataa gaccaatctc tttaaaacct gcaagtctag caaggtctac tgaggcttct
    11521 gtatgtccat ccctgacaag aactccacca gtttttgcta ttaaaggaaa tatgtgacca
    11581 ggcgttttga aatctttact tgtagaattt tcatcacaga acaattttac agtttcagct
    11641 ctttcatatg cagatatacc agttagagta tctgctccat ctatagtaac agtaaaggct
    11701 gtttgaaatg tatctgtatt ttctctaacc ataagaggta tattaagact tttaaatttt
    11761 ttttcagtgg caggtagaca aattagacct ctaccatatg tagccataaa atttattgat
    11821 tcaggagtag ccatttcagc tgccatcaat aaatctcctt cattctctct actttcatca
    11881 tcaactacga ttaccatttt cccatttttt atatcttcaa tagcttcttc aatagtatta
    11941 aacatgtaat tcactccttt attattttta aaaaccattt ttaaataaaa aatctttatc
    12001 tatatttgat ttatggttac tactattaga atttaattcc ttgtagttat tagccataaa
    12061 attattaaca tactttccaa ttacatcgct ttctaaatta acaaaatctc caacattctt
    12121 tgtcaagaga atagtgttaa ttttagtatg aggtataata gaaaccttaa atatttcatc
    12181 atctaaatat gatatagtta aacttacacc atcaatagca acagaacctt tgagtatcat
    12241 tgaagataaa agttttttat caggtcgtat acttactaaa actgcatttt catctttttc
    12301 aaatttgcag atagttcctt taccatcaac atgacctgtt actatatgac caccaaatct
    12361 tgaagaaaga ctcatagctc tttcaagatt taccttatca tttgcattta ataaacctaa
    12421 atttgtggaa cgaattgtct ccatcataac atcggctgta aattcatttt ttccgaggtt
    12481 tgaaacagtt agacaaactc catttacggc tatactatct ccaagatttg tgccatctaa
    12541 tattttattt gatagtatgg ttacttttcc actcttacca ttagcagtaa tttttctaag
    12601 tattccaact tcttctacta tgccagtaaa catcctaacc tcctatatga atgtattaac
    12661 aaaaatttaa tttaaaacat ctgattctat aagaatatcg ccatctatca aagttacatt
    12721 attgattgtt aactgtgttg aatctgacaa atgatttatt ccttctccag aaacgaatgt
    12781 aggtgcatct ttaccaccta taatcttggg tgatagaaat aatttgactt tatcaactaa
    12841 cttatttcta aagagactgt cattgagagt agcaccacct tcaactagaa cagaatcaat
    12901 gtttaactct cctaatttat acattacaaa ttccaaatct actaagttgt tttttgaagg
    12961 agaaattatt gtttcaacat tattttcttt taacatagat aattttatac tttctttgca
    13021 acatgtgaaa actatagttt tactggtttt atctttaacc aaattactat taagtggcaa
    13081 ctttaggtgt gtatctatta ctattcttgt aggatgagac actttttctt gattgagcct
    13141 gcaagttagt aaaggattat cgttcaaaac tgtatttatg ccaaccatta tactttggta
    13201 tttatttctg tatttatgag ttagatacct tgatgattca ttagatatcc attttgattc
    13261 tagtgatttt gtagcaattt taccatctag acttactgct gattttacaa tacaaagagg
    13321 tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa tctcttgctt catcttctaa
    13381 tattcccaca gtaacatcaa tattgttatc ttttaatttt tttactccat taccacaaac
    13441 aagtggattt gggtctaatg tagatattac aactctctta atcttatttt gtataatttt
    13501 atctacacaa ggaggcgttt tcccataatg agaacatggt tctaagttta cataaagagt
    13561 agagtctttt agactttgtt tagcactgtt tatagcattt acttcagcat gattagagcc
    13621 aaatttctca tgatatcctt tgcctattat attactatct ttaacaatta cacacccaac
    13681 taatggattt ggatttacaa agccttctcc atttttagcc aattcaatag cttttttcat
    13741 ataataaata tctttttctt tttgattcac aatatgtaca cctcctgttt aattggataa
    13801 tttttaaaca aaaaaccctg agaattaact cagggtttag aataatctac aataaatgtt
    13861 taataaaagt agaagaaaat tgctctacaa aaacactcat taaatatctt cttccatcca
    13921 gactttactg tcggctttgg aatttaacca aatcaaccaa gttttacctt ggctcgcggg
    13981 ctataccgcc gattgggaat ttcaccctac cccgaagatt aagtattaaa tttatattta
    14041 gaatgtatca taaattattt tactttgcaa actatttttt taaaaaatat atgttaatat
    14101 ataatataga atgtctataa actttgaaat accacgattg tgaatataaa aataaaaatt
    14161 tgaacataat caggaaagaa gtctaattaa tttaaattag gagagtaata tatgaataaa
    14221 aaacttttag aaatactatc aaaatactat ggatatacaa gttttagaaa aggtcaggaa
    14281 tctataataa attctatctt aagcgaaaaa gatgtgttag ctattatgcc aacaggaggg
    14341 gggaaatcaa tatgttatca aattccagct ttgattttag atggaatgac aatagtaatt
    14401 tctccactta tatcacttat gaaagaccaa gtagatgcct taaagactat gggaattagt
    14461 gctgctttta taaatagttc attaagcagc aaagaattta atgaaatatt aaataatatt
    14521 agaaaaaata attataaaat actctatatt gctccagaga gattagatgc acaagagttt
    14581 attgagctaa taaataataa caatgtaagt caagttgcga tagatgaagc acattgtgta
    14641 tctcaatggg ggcatgattt taggttgagt tatagaagga tttctgattt tataaaaaat
    14701 ttgcctaaaa gacctatagt tacagcattt actgcaacag cttcagaaga ggtaagaact
    14761 gatataataa atttactgtg tttagaaaat ccagattatt atataactgg atttgataga
    14821 gagaatttat caataaatat agtaaaatca tctagtaaaa ataagtatat tttagattat
    14881 attcaaaatc ataaaaatga gtctggaatt atatatgttg caactagaaa ggaagttgaa
    14941 aatatatata atggtttatc taagagaaat atatctgtat caaaatatca tgcaggttta
    15001 tctaaaaatg aaagaagtaa aaatcaagaa gattttataa atgataatgt ggatataatg
    15061 gtagcaacaa atgcatttgg aatgggtatt gataaaccta atataagatg ggttatacat
    15121 tacaatatgc ctcaaagtat agaaaactac tatcaagaaa taggtagagc tggtagggat
    15181 ggagaaaaaa gtgagtgtgt acttttattt tctccaggag atgtacatat acaaaaatat
    15241 ttaattgata ttggagttga aaatcctgaa agaaaattat ttcagcataa gaagcttcaa
    15301 tacatgattg acttagttta cagtaattca tgctatagaa agacaatatt gagttatttt
    15361 ggagaaaatt atatagacaa ttgcaataat tgtagcaatt gtttagtaga gggtgaaata
    15421 gtagataaaa ctatagatgc acaaaaagtc atttcatgtg tagctagaat gaaaagaagt
    15481 tatggtgtta ctactattgt agatgtactt agaggttcta agaataaaaa gattttacag
    15541 cttggattaa atactttgtc tacatataac attatgagag attattctag tgaagactta
    15601 aagaattttg taaatacatt ggtttctcat ggttttttag atttagttga gactctaggc
    15661 aatggaaata gaggtagttt tcctactata agattaaatg agatgtctat gaaagtttta
    15721 aaaggtgaag tgaaagtaga gtttaaagaa attgttatga caaaatcatt ggaagtagag
    15781 gatgagttat ataatgcttt aagagaactt agacattcaa ttgcaagtga agaaaggata
    15841 gctccatata tggtatttgg agatggtaca cttagagcca tgtctagtag ttatccaatt
    15901 aataaagaag aaatgctaga tatctctgga gtaggagaaa ttaaatatca aaaatatggt
    15961 agagattttg aagcagtaat taaagaatat gtcgaaaaaa ataatataaa taaaaaaata
    16021 gatgaaaaaa atgtcgattt aaagaacgtt aatagtgatt tcttagaggt taatacagat
    16081 aaagttttat atgaaaaact gttagtcatt agagaagaat atgcaacaaa ggaaaaatta
    16141 cctgcatata tgattttagc caataaggct ttgaaagaaa taagtggaag atatccactt
    16201 gatgaagaac aattaaaaga tatttctggc ataggtggag taaaatttga aaaatatgga
    16261 gctaatattt tggcagaggt taataaatat gtaaaagaaa ataatattaa tgttatttgg
    16321 gagaaaaagg gcaaaaggaa gttaatatta gatggagaaa gtagaaaaaa taatgaaata
    16381 gcattagatt tgctaaatca aggaaaagat atacaaagta tcagtcaaga aatagaagtt
    16441 tcattgtcaa ccattattgg atatgtgcat gactatgtaa aggatggaca tagtatatca
    16501 tttgaattag gactagaaaa tttttataat gaagataaaa agaagatgat tttagaagtt
    16561 tgcgaaaaag ttggctatga taatttaaat aacataaaaa gaaaacttcc agattcaata
    16621 aagtatgaaa atataagagc tgtgatgctt gactcttatc ttgaaaactt aaaaaataat
    16681 attatagcag agtaattttt aacttaataa aaataaaatt tgtattattg tgttgaacta
    16741 ataattttgc tgtgttatac tgaatttgta aaaataatga aaaattagag ctaggggcgc
    16801 tagaattggc tagctgagag ataaagccgt aacttacaaa ccctaataac ctgatcaaga
    16861 taatgcttgc gaagggaagc tttttaatat aattttatta tataaatagg caaattattc
    16921 aatgttagga gaaatttgtc taagtgtata tacgattata agagtgcttt ccaaacgagg
    16981 aaaagcactt tttttatttt caaaaactat aactttaatc ttggaggatt gtaatatgaa
    17041 ctatacaaca caaatggatg ctgctagaaa aggcataata actaaagaaa tggaaatcgt
    17101 ttctcaaaaa gaacaggtag atgtaaatga attaagagaa ttaatagcaa atggacaagt
    17161 tgttatacca gctaataaaa atcataaatc tttaagtgca gaaggtgtag gtaaaaattt
    17221 aagaacaaaa ataaatgtaa atctaggaat ttcaagagat tgtaaggata tagaaaaaga
    17281 acttgaaaaa gttagagtag caatagacat gaaagctgaa gctataatgg atttaagtaa
    17341 ttatggcaaa actagagaat ttagagaaaa ggttgttgaa atgtcacctg caatgatagg
    17401 ttcagtacct atgtatgatg cagtaggata tttggaaaaa gaattaaaag atataacaga
    17461 agaagagttt ttaaatgtaa taagacaaca tgctattgat ggagtagact ttattactat
    17521 acatgcaggt cttacaagaa gtgtttgcca aaaaataaaa aatcatgaga gattgactca
    17581 tatagtatct cgtggaggtt ctttattgtt tgcatggatg gaattaaata ataaggaaaa
    17641 tcctatttat actaattttg ataaaatatt ggatatatgt gaagaatatg atgttacatt
    17701 gagtttagga gatgcttgta gaccaggttg tataaaagat tctactgatg gtgttcaaat
    17761 tcaagaactt gtagtattag gtgaacttac taaaagagca tgggaaagaa atgtacaagt
    17821 aatgatagaa ggtcctggac acatggctat tgatgaaata gaagcaaatg tagtattaga
    17881 aaagagatta tgccatggag caccttttta tgttcttgga ccattggtta cagatatagc
    17941 accaggttat gaccatataa caagtgcaat aggaggagct ttagcatgtg ctaaaggagt
    18001 agatttttta tgttatgtaa ctccagcaga acatttaaga ttgcctaatt tagatgatat
    18061 gaaagagggt ataatagctg caaagatagc tgctcatgca ggagatatag ctaaaaatgt
    18121 taaaggtgct cgtgaatggg ataataagat gagtaaagct agagctgatt tagactggtg
    18181 tgagatgttt agacttgcaa tagaccctga aaaagctaag agatacagag atgaatcaac
    18241 tcctactcat gaggatagtt gtactatgtg tggaaaaatg tgctcaatga gaacagttaa
    18301 gaaaatatta aacaatgagg aacttaatct aatataattt ttggctagta tgactggaat
    18361 aaaaagaagc tgaaaagagg aggtgattta gtgaaggtta atgggaagga aattgaattt
    18421 gaaaaagatt tgactgttat agatttatta aataaatata atttaaaaag tgatagagtt
    18481 gtagttgaag tgaatttaga gataattgaa gaatctaact acaatacata tgtattaaaa
    18541 gacgaggaca ttgtagagct tattagcttt attggaggtg gttaaaataa aaatatttgt
    18601 aaatgaatta ttaacagatg ttgaggaaaa tacaactgct tataaagtta gagatagcta
    18661 taacaataaa tgtgatgtat tggtgttaaa tggatatcca attaaaacag atatgcccct
    18721 ttgtgaaaat gataaattaa cacttataca aaaaggagtt aagccatcac ttgatgaatt
    18781 ggaaagcttg ttgattgcta gacatactcc aaatatacat aataaattaa aaaaagggaa
    18841 agttgctata ttgggattgg ggggacttgg ttccaatata gctatctctt tagctagaat
    18901 aggagtggga gaacttttac ttatagatta tgatgttgta gaaccatcta atttaaatag
    18961 acaacagtat tttatagatg atataggaaa actaaaaact gatgccatga tagaaaatat
    19021 aaaaaaaata aatccattta taaaattaaa taaacgagat atttttttaa acaagaacaa
    19081 tatggatact attaaggatt cagatttaat aattgaggcc tttgatgatg cttcatgtaa
    19141 agcacaggta tgtaattatg tattaataaa tttaaaagat aaatatttaa tagcatcatc
    19201 tggtatggca ggttactatg attcaaatat aataactact aagaagataa aagacaggtt
    19261 ttatatatgt ggggattttg tgaatgaagc taaatttggt gaagggctta tggctccaag
    19321 ggttgccatc tgcgcaaatc atatggcaaa tttggcaacg caaatactta tagatatgta
    19381 aatattaaaa ttagatataa aaaagattga atattaaaaa aatttaacgt ggagggaaat
    19441 aatatggata aattggtact tggtggacat gaatttaata gcagattatt ggttggaaca
    19501 ggtaaatatg gttcaaataa tatattgcct gaggtaataa aagaaagtgg aagtgaaata
    19561 ataacaatgg cacttagaag agtggattta gacaataaac aagaaaacat attaacgtat
    19621 attccaaaag agatgacaat acttccaaat acatcaggag ctactaatgc agaagaagct
    19681 gtaagaatag ctagaatatc tagaaaaatg ggatgtggag attttataaa aatagaagta
    19741 atatcagata ctagatattt acttcctgat aatgaagaaa ctataaaagc tacaaagata
    19801 cttgctgatg aggggtttat agttcttcca tatatgacac cagaccttta tgctggaaga
    19861 agacttatag aagctaatgc agccgcagtt atgccacttg gagctccaat tggttcaaat
    19921 agaggtcttc aaatgaaaga aatgataaga ataatgatag atgagttgga tataccaata
    19981 atagtagatg caggtattgg aaaaccatct caagctatgg aggctatgga aatgggagct
    20041 gatgcagtac ttgtcaacac tgctatagct tcagcaggag accctgtaca aatggctaga
    20101 gcatttaaat tagctgtaga aggtggaaga gaagcatata ttgcaaaaac aggaaatgtg
    20161 tcagaatttg caaatgcatc ttctccttta acaggttttt taggcaattt atagaaagtg
    20221 gtgattggtg atgagttttt atgatgtaat agaaaaatat agggattttg attttgatgg
    20281 atatttaaat aatgttactg ataatgatgt tttaagaagt ttatcaaaag acaagcttga
    20341 agattttgat atattaaatt tgctttctaa aactgcagta aaacatttag aagatatggc
    20401 acaaaaagca cataaattaa gtgttcaata ctttggtaaa acagtatgtt tatatacacc
    20461 aatgtatata gcaaattatt gtgttaatca atgtgtttat tgtagctata atataaagag
    20521 tggaataaaa agaaaaaaat taactatgga tgaaattaga gaagagggag aagcaatttc
    20581 aaaagaaggt ttcaaacatc tacttgtttt gactggagaa agtagttttc actcaagtgt
    20641 agaatatata ggtgaggcta tagaaatatt aagagagaaa tttccctcta taggtataga
    20701 agtttatcca atggaagtag aagaatataa gtacattgta gataaaggtg ttgaaggatt
    20761 aacagtatat caagaaactt atgacgagga aatatataag agagtacata taaaaggccc
    20821 aaaatcaaat tatgaattta gactagatgc tcctgaaaga ggggcaaaag caggtatgag
    20881 gactttatca ataggtgcac ttttaggtct aaatgatttt agaaaagaaa ctttttttac
    20941 aatattgcat gggaaatact taaaaacgaa atatcctcat attgagcttt catattcaac
    21001 accaagaatg agacctttta aagggtgttt tgaggaattg gtagatataa gtgatactga
    21061 tttagttcaa gctatggtat gtatgagatt atttgaccct catgcagcta taaatatatc
    21121 cactagagaa aatttagaaa tgagaagtca tataatacct ttaggagtaa ctaaattgag
    21181 cgcaggtgta tctactgatg taggaggtca ttctcaagat gaacatgata ctgctcaatt
    21241 taagataaat gatgaaagta ctgttaaaga tgtggagaaa atgctaaact caattggtta
    21301 tcagcatgtg tttaaagatt gggaaagatt ttaaatgtat ctgataacaa acagaaaatt
    21361 atgtagtgag gaacgatatt tagaagttat taaagagtct atattgagtg gtgtagaaaa
    21421 tataataata agagaaaagg atttggaata tcaagagcta agaaaattat atatgaaaat
    21481 aaaaactaaa atcaattgta tagattttca agagcaaata tcagatgaaa gtttgaaaac
    21541 taatataaat caaaaagagt gtagaaataa attcaaggtt aattttataa taaatagtaa
    21601 cattgaattt tttgaaaaag tggattgtca aggtatccat cttcctttta aattattctt
    21661 gaatttaatt gaaaataagt ataattttaa tgaaaataaa atactaggat tatctttaca
    21721 taaagtagag gaagtagatt atttagaaaa attaataaga aatcaaaata taaaaataga
    21781 ttacataaca ttatcacata tatatgaaac taaatgtaaa gaaggattaa atccaaaagg
    21841 aattgagctt ttaaaagaag ctaaaaagat tactgatatt aaaataattg cacttggggg
    21901 aattttacca tctaatgtca aagaaacatt aaaatactgc gatgattttg ctataatgtc
    21961 aactataatg aggtctaaag atattaaaaa aaccatatca aattataatg aaaagttgaa
    22021 ttagtaaata tagtatattg aatttttaac ttaaataaaa ataaaaaagt tatcttttaa
    22081 attttaaaag gtagcttttt tattttatat ctttgctaat aaaaaattgt aaaaagggaa
    22141 aactttagtc agagagatta aacaatatcc tagaaagatt taaaatttag taaagtatgg
    22201 aggacattat aatgactgac agtaaaaaga aacgatttat tataccacac acttttacaa
    22261 taatattttt actaatagtc ttgatggcaa tattgacttg gatagtacca agtggtgaat
    22321 ttagtagaga agatgtagaa ggtagaatgg tagttgtacc agggacttat caaagtgtag
    22381 aaagtaatcc tcaaggattt gcagatgttt ttaaagcacc aatagaaggc tttatagatt
    22441 cagctgaagt tgtaggtttt gttttaatag ttggaggtgc ttttggtatt gtaaatagga
    22501 ctggagctat agaagcagga atagcttcgg ttatctctaa gtttaaggga aaagaacttc
    22561 ttataattcc aatatcaatg attttatttg gacttggagg tacaacattt gggatggcag
    22621 aagagacatt accattttat atgatatttg ttccactcat gacatccttg ggatatgatt
    22681 ctctaacagc aatagcaatt gtatttatag gagcagcagc aggagccgcc gcttcaacaa
    22741 taaatccatt ttcagttggt attgcacagg cactagctca acttgctcct ggttcaggag
    22801 taacttacag agtagttatt tttatagcat atatactaat aagtatagca tttgttatgt
    22861 actatgctaa gaaggtaaag gaaaatcctg aaagttcaat agtatatgaa atagataaaa
    22921 ataataaatc aatacttaat caaacttcaa cagaaataaa agaatttact tttaaagaag
    22981 tatctgttct tgctatattt ggaataggga tggcaataat gatttggggt gttttaagtc
    23041 aaggatggta cattccagaa atatcaatgt gttttgctgc aataggtgtg ctttctggat
    23101 tggttggtag attatctcaa attgaaatct cagaaagttt tatagatgga gcaaaagatt
    23161 tagcatctgc agctcttgct atagctttgg ctcgtggaat cgtaataatt gctcaaaatg
    23221 gatttataat agatactata ttaaattccg cagcagcctt tctaggtggg cttccaaagg
    23281 ttatatttgt atatttatca tttttcttgg aagctgcatt ggcattttta attccatcat
    23341 cttcaggtct tgcttcactt acagtacctg tacttgctcc tcttggtgat ttggttaata
    23401 tatcggctca agtgatagta accgcttatc aatttggtgt gggtcttaca aactttataa
    23461 ctccaacttc aggagtactg atgggagcac ttgcagtagc ccatattcca ttttcaaaat
    23521 gggtaagatt tataatgcct atcttgttga ttttgacagc tgtatctcta gtctttttaa
    23581 caataggtat ttatataggg ttttaattaa gtgtagtttt atttaagata tttagtaatg
    23641 gtataaaatg ataatatgtt ttcaataaat tgccactaat ttaattagtg gtaatttatt
    23701 ttttgtattt aaaaataaat gtatttactt gt
//
LOCUS       DAEPOG010000045        23607 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEPOG010000045 DAEPOG010000000
VERSION     DAEPOG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.045"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            46..417
                     /locus_tag="KR432_001503"
     CDS             46..417
                     /locus_tag="KR432_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0640047.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            549..1823
                     /locus_tag="KR432_001504"
     CDS             549..1823
                     /locus_tag="KR432_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0640048.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1934..2878
                     /locus_tag="KR432_001505"
     CDS             1934..2878
                     /locus_tag="KR432_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640049.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3094..4431
                     /gene="rsxC"
                     /locus_tag="KR432_001506"
     CDS             3094..4431
                     /gene="rsxC"
                     /locus_tag="KR432_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH0640050.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4454..5431
                     /locus_tag="KR432_001507"
     CDS             4454..5431
                     /locus_tag="KR432_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBH0640051.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5431..6000
                     /locus_tag="KR432_001508"
     CDS             5431..6000
                     /locus_tag="KR432_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBH0640052.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            6013..6606
                     /locus_tag="KR432_001509"
     CDS             6013..6606
                     /locus_tag="KR432_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBH0640053.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6621..7196
                     /gene="rsxA"
                     /locus_tag="KR432_001510"
     CDS             6621..7196
                     /gene="rsxA"
                     /locus_tag="KR432_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH0640054.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7212..8183
                     /locus_tag="KR432_001511"
     CDS             7212..8183
                     /locus_tag="KR432_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBH0640055.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8403..8987
                     /gene="maf"
                     /locus_tag="KR432_001512"
     CDS             8403..8987
                     /gene="maf"
                     /locus_tag="KR432_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBH0640056.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9032..9691
                     /gene="radC"
                     /locus_tag="KR432_001513"
     CDS             9032..9691
                     /gene="radC"
                     /locus_tag="KR432_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH0640057.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9812..10822
                     /locus_tag="KR432_001514"
     CDS             9812..10822
                     /locus_tag="KR432_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0640058.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            10832..11725
                     /gene="mreC"
                     /locus_tag="KR432_001515"
     CDS             10832..11725
                     /gene="mreC"
                     /locus_tag="KR432_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH0640059.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11740..12228
                     /gene="mreD"
                     /locus_tag="KR432_001516"
     CDS             11740..12228
                     /gene="mreD"
                     /locus_tag="KR432_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH0640060.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12240..15218
                     /locus_tag="KR432_001517"
     CDS             12240..15218
                     /locus_tag="KR432_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBH0640061.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15316..15999
                     /locus_tag="KR432_001518"
     CDS             15316..15999
                     /locus_tag="KR432_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH0640062.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            16023..16820
                     /gene="minD"
                     /locus_tag="KR432_001519"
     CDS             16023..16820
                     /gene="minD"
                     /locus_tag="KR432_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH0640063.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16838..17122
                     /gene="minE"
                     /locus_tag="KR432_001520"
     CDS             16838..17122
                     /gene="minE"
                     /locus_tag="KR432_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH0640064.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17207..18337
                     /gene="rodA"
                     /locus_tag="KR432_001521"
     CDS             17207..18337
                     /gene="rodA"
                     /locus_tag="KR432_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBH0640065.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18378..18791
                     /locus_tag="KR432_001522"
     CDS             18378..18791
                     /locus_tag="KR432_001522"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH0640066.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            19276..19605
                     /locus_tag="KR432_001523"
     CDS             19276..19605
                     /locus_tag="KR432_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640067.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            19609..20400
                     /locus_tag="KR432_001524"
     CDS             19609..20400
                     /locus_tag="KR432_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640068.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            20964..22046
                     /locus_tag="KR432_001525"
     CDS             20964..22046
                     /locus_tag="KR432_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0640069.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(22258..23451)
                     /locus_tag="KR432_001526"
     CDS             complement(22258..23451)
                     /locus_tag="KR432_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBH0640070.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT         9035 a         2769 c         4542 g         7261 t
ORIGIN      
        1 aatgatataa tataaaaaaa ataaaaattg gagagtgatt tcaaaatgaa gtttaatttt
       61 tgccataaca atttcaatgt tactaactta gaaaaaagtt tagattttta taaaaaagct
      121 ttagggctta aagaggtaaa gcgtaaggaa gcagaagatg gaagttttat attagtttat
      181 ttaggagatg gtattactag tcatacatta gaattaactt ggcttagaga ttgggacaga
      241 ccatataatt taggagacaa tgaatttcat ctggctttag aagttgagga gtttgatgag
      301 gcaaaaaaac ttcataaaga tttagattgc atatgttttg aaaatgaaag tatgggaatt
      361 tattttatag cggacccaga taattattgg attgaaatac ttcctaaaaa tcattaattt
      421 taaggatatg tttataattt ctttacaatt aataagagaa taagttatca ttaaattgta
      481 gggactaaaa atggataata atgttatgaa tttatgacta taaaaatttg ttggggggag
      541 tgatagtagt gaagaaagct atagcagctt taggaattgg ggcagtagca gtatctgtta
      601 gctccataaa tgcaagtgct cttgaaaagg gaacagtaac agcaagtgcc ttgaatataa
      661 gaagtggacc aagttctgat tgtgataagg tagcaaaatt atacaaggga aaaactgtag
      721 agattttaga aaagtctaat ggatggtata aagtaagggt atcgagttct gttgttggat
      781 ggggaagcgc aaaatacata tcaacaagtg gttcatctga aggtacatca aatcaaaata
      841 atccaacttc aagtgggaca actattagtg gaaatggtaa agtaaatgtg agttctagac
      901 tgaatgtaag aagtggtgca ggtactaact attcattagt tggtaaagca aataatggag
      961 atgtagtaaa gttactagaa caaagcaatg gatggtataa aataaaacta tcaaatggag
     1021 ttactggatg ggctagtagc caatatatct ctaaaacatc agaggatgtt ggaacgaata
     1081 attctagtaa ttcaaatagt actaataatt ctgataaaaa accatctagc gaagaatcaa
     1141 ttgaaggcaa gaatggtaag gtaacatctg ctgtgagttt aaatgtaaga agtggtcctg
     1201 ggacaagcta ttctataata ggaaaattaa atggtggaga tgttgttgaa ttaaaatcca
     1261 aaaataatgg ttggtataag gtaaagttat cgagtggaac aattggatgg gtgagtgcca
     1321 gttatatttc tgagactaat gaaggtacaa aagaaaaacc aaattcatca tctaaccaaa
     1381 attctcaatc taatagtaac tcaaagccat catttactgg aaattcagat aaatctacag
     1441 caaaaggttc aacaatcgta gattttgcat atactcttat tggaatacca tatcaatggg
     1501 gagcttcagg acctgataaa tttgactgtt ctggatttac acagtatgtt tttaaacatt
     1561 ctgtaggcgt ttctattcct agagtttcta gagaacaggc taattttgga agtgcaattt
     1621 ctatggggaa ttatgcacca ggagatttag tttattttga cactgatggt gatggaacaa
     1681 ctaatcatgt aggtatatat gtaggaaata gtaaatttat ccattgtagt ggtactcaga
     1741 ctaatcctaa taaggttaaa gtggataatt taactagttc atattggtct aaagtattac
     1801 ttggtgcaag aagatttgtt tagtaagtac aatttcttat taaaaaatta gaagaaataa
     1861 ttatttatat tcataaattg tgactcttaa aaatatactt tattaataat ataattttgg
     1921 gagggacaaa ctaatgaata gaagaaaaac gatatacaga ggatttaata ataatagaca
     1981 taagattgat ttacgtaaat atgttataac gattgcttgc ttgtgtctaa ttggctatta
     2041 ttcttataca aaaataaaag atagcaagat attggagtat gtatcggcta aaataccatt
     2101 tctaaataat tcatcagata taacttataa agatatatct gatgaattaa attcaataaa
     2161 aaaagggaaa aaatctaaat cacaaaccaa ttcagatgat aaacaagaaa ctaatccaga
     2221 aaaagcggta aataacacta aagagccaga agaagtcaaa ttagctacta tagaaggctg
     2281 ggatatgtac actattcaag ttgctgcaat agataataat gatgatttga aaaaaataca
     2341 gacatcatta gttaataatg acataccttt ttcagttatg gagaaggatg gagtaaaaaa
     2401 aatacagacc tattcctctt ttgatgaaaa tgatgttaga aagcagataa gcagtgtaag
     2461 aaaggtgttt ccagatgcat ttttatcaca cttagatgca cctatgttgt ctttagagta
     2521 tacaagtaac tacgcttata tagagagtat atctaaagaa ttaaataaat taataacaaa
     2581 ctttaaagaa gaatcaagtt tttggtcgaa tgctgaaaat aatgtagata tggaaaaata
     2641 taatactatt ttgacaaata gaaaggcaat atcacaaaat atatcaaaag aagctgaaaa
     2701 gattgactat tctgaaatgc gtttatttaa agataattta atagagtatg taaagaatgt
     2761 gaatgaaaaa attgatacag cttcaaaagc agcaaatgaa gaaaaatata gtgtaagtaa
     2821 aagtctgctt ttatcatcca tgcaggagta ctctatgttt ataaattcta taaaataaaa
     2881 tacagaaaat ttggacttta gaaagctata tgaaaaggat tgtttaatta agcaatcctt
     2941 ttcctcagtt tgacttaata tagaggtatt gatttataaa ataaattata aaaatacgga
     3001 tattttttta acaattgtta agatttttta atatatatag tatacttata gaaggcagaa
     3061 aaaatgtttt tttatagata agggggtaaa acaatgaaac tcttaacttt taagggaggg
     3121 atacatcctc catatagaaa agagtactct aacacaaagg cacttgaaaa agctcaagct
     3181 ccaaagatag tttacattcc tcttcagcaa catatagggg caccagctaa acctattgtt
     3241 gaagttggtg atgaagtaaa gtttgggcaa aaaataggtg aacaacaagg ttttgtttca
     3301 tgtaacgttc attcttcagt atcaggtaaa gttattgcta ttgaacaaca tgaagtacca
     3361 ggaggttcag ctcaatgtgt agttatcgaa aatgacttta aggaagaatt acatgagagt
     3421 gttcaaccaa aaggtcagtt agaggatttg agcaaagaag atatagtagg tatcataaaa
     3481 gaggcaggaa tagtaggtat gggtggagct acattcccta accatgtaaa agtatctcct
     3541 ccaccagata gcaaggcaga agtagttata ttaaatggtg cagaatgtga accatactta
     3601 actgcagacc acagattaat ggttgaaaat ccagaagatg ttgtgtttgg tttaagagca
     3661 ttaatgaagg tattagatgt caaaaaagga tttataggaa ttgaaacgaa taaaccagat
     3721 gctatagaag caattcaaaa tgtagctaaa gactatagtg aaatcgaagt tgttggcctt
     3781 caagtgaaat atccacaagg agcagagaaa caacttattt atgcgtgtac aggcaaagaa
     3841 gttccatctg gtggattacc aatagcagca ggtgcagttg ttgacaatgt tgccacagca
     3901 gcacaaatag ctaagtctat taaaacaggt atgcctttgg ttgaaagaat cactacaata
     3961 acaggtagtt gcatcaaaga gccaaagaac ctaataacaa aagttggaac attagtttca
     4021 gaaataatag aacaatgtgg tggatttaaa gaagataaga agattggtaa ggttataatg
     4081 ggtggaccta tgatgggaat agctcaatat actactgaaa tagcgactaa caaaggttct
     4141 tcaggaattt tatgcttgga tgaggaagaa tcacgtactc cagatattca aaactgttta
     4201 agatgtggaa gatgtacaga tgtatgtcca tcattcttac aaccactttt cataagtgca
     4261 tattctttaa aagatgacta tgacacagct gaatatcata gagcaatgga ttgtatagaa
     4321 tgtgggtctt gttcatttat ttgtccagca agaagaccat tacttcaatc tataagatca
     4381 gcaaagagag aaataggagc aaagagaaga aagcaagctg ctcagaaata aagaaagtta
     4441 agggggaaaa acgatggaaa ataagttgat agtatcatct tctcctcatg tgagaagtaa
     4501 tgaagatact tcatatataa tgaaacaagt tattatagca ctccttccag cagcagtagc
     4561 aggagtatac ttctttagac ttaatgcttt gagtgctatg tttttttgta tacttggtac
     4621 agtaggtact gaatttttat atcaaaaatt tacgaagcaa aaaagtacta taggagattt
     4681 ttcagctgtt gtaacaggat tattattagc atttaacgta ccagcatcac ttccttggtg
     4741 gatgtgtcta gtaggaggaa tatttgcaat attagtagtt aaaatggtat ttggtggaat
     4801 tggatgtaac tttgtcaatc cagcacttgc tgcaagagca tttttattag catcattccc
     4861 agtagcaatg actgcttgga ctcaaccagg tgttaactgg ataggtaaaa atttagatgc
     4921 agttactaca gcaactccat taagcttttt gaaaaatgga gcagcaggac tggcagacct
     4981 ttctagcaat ggaattagtc ttgctgacat gatgataggg aatattggtg gatgtatagg
     5041 tgagacatca gcaatattat tattattagg tggagtatac ctaatgtata aaggcataat
     5101 aaattatgtt ataccagtat tttacatagc aactgtattt atattaacat tccttttagg
     5161 tggatttaat ataactttcg caatatatca actgtttgct ggaggactta tgttaggtgg
     5221 attcttcatg cttacggatt atacaacttc gcctatgact aaaaaaggtc aaataatata
     5281 cgctgtatta gctggtctta taacaactgt tataagaatg tatggtggat atccagaagg
     5341 tgtatcttac tcaatactat tagtgaactg tcttgctcca cttatagata agtttgttag
     5401 aaatagagta tttggggagg tggctaaata atgaacagta tggtgagatt aggtggtact
     5461 ttacttgcta taagtgcaat agctgcatta gctcttggag ctactaatca ggttacagct
     5521 ccagtaatag aacaaagaaa tatacaagaa aataatgaac ttagaaaagc agtccttcca
     5581 gaagcaaaag aatttaaaga aatggataaa agtacatata aagaccttgg agatggtctt
     5641 atagctgaag tgtatgaagg tttagatggt tctgaagtgg ttggatatac tttaaaagct
     5701 aaaccaagtg gatatggtgg agaaattgaa gttatggtag gtatatcatc tgaaggtcaa
     5761 gttacaggtg ttgacattgg taatatgtca gaaacagcag gtcttggagc aaaagctaag
     5821 gatgatgcat ttaagggtca atacaaagga aaaactgctg agcctcttga agtagcaaaa
     5881 ggaagtacaa cagcagataa tcaaatatta gcaatatcag gtgcaactat tacatctaca
     5941 gcagttacta caggagtaaa tgctgctata gatgttttca atagtgctct taataaataa
     6001 ggagggttaa ttatgaattt agctaaagta tttaaaaatg ggctaataga tgagaaccca
     6061 acatttgttc aagttatagg tatgtgtcca acactagccg ttacaacttc agcaataaat
     6121 ggaattggta tgggtctttc aacagcagca gttttaatat gtgcaaattt agttatatct
     6181 ttaataagaa aaattacacc agataagata agaataccta tatttatagt agttatagct
     6241 acatttgtta caatagtagg tatgctttta aaagcatatg ttcctgctct tgataaggct
     6301 cttggtatat atataccatt aatagttgtt aactgtttaa tattagctcg tgcagaaagt
     6361 tttgctttca aaacaggagc tatgccttca atagttgatg gtgtaggtca aggtcttgga
     6421 tttactgttg cacttacaat aataggtgca gtaagagaat tacttggaaa tggtagttta
     6481 tttggaatga cattatttgg agcatctttc caaccagtat taatatttat attaccacca
     6541 ggtgcattct taacattagg attcttattt gcaggattta ataaattaag aagtaaaaaa
     6601 gcatagtgga ggtgtaataa atgaatttaa tacttttatt tttaagtatc gttcttgtta
     6661 ataacgttat aacatctcaa ttcttgggta tatgtccttt cttgggagtt tctaagaagg
     6721 ttgatacagc agtagggatg ggagttgcgg ttacgttcgt tttaactcta gcttcaatta
     6781 taacttattt tatacagatt ttattagtaa aaactggaac tggattctta caaacaatag
     6841 cttttatatt ggttatagct tctatagttc agttcgttga aatggtaatt caaaagatga
     6901 gtccatcttt atatcaagca ttaggagttt atttacctct tataactaca aactgtgcag
     6961 ttcttggtat agctctagtt aatgttcaaa aaagttataa tttagtagaa acaataataa
     7021 atggatttgg agccggagct ggatttactt tagcaatagt aatattcgca ggtataagag
     7081 aaagactaga gttagctgat ataccagaag catttaaagg tttccctata acgcttatat
     7141 cagcaggttt aatgtcaata gctttcttag gatttacagg acttataaag ctataaaaaa
     7201 gaggggtgaa aatggtgata cttacagctg tattagtttt aggaattatg ggcttaatct
     7261 tcgggatagt actagatttc gcatctaaaa agtttgctgt agaagtagac gagagagtgg
     7321 aagctatact aggagtactt ccaggagcca actgtggagg ttgtggattt ccaggttgtg
     7381 gaggtcttgc aaatgcgata gttgaaggca atgcaccagt taatggttgc cctgtaggtg
     7441 gagcagatgt tggtgcaaaa gttggagaaa taatgggaat aagtgctgaa gcaggtgaga
     7501 aacaagtagc aaaagttatt tgtaaaggaa cttgttcaag tgcaaaagat aaatatgagt
     7561 atgaaggaat atcagattgt agagcagcaa atgttttaaa ctcaggtgct aagatgtgta
     7621 aatttggatg tttaggtctt ggaacatgta aggatgcttg taaatttgat gctatttcta
     7681 tagttgatgg aatagcagta atagatgaag aaaagtgtgt aaattgtgga aaatgtaaag
     7741 aagtttgtcc taagggtata ataatcacaa aaccagaaag tcaagaagta gttgtagagt
     7801 gtaatagcaa agagtttggt aaagctgtta aggaaaaatg tactgctgga tgtattggct
     7861 gtggaatgtg tgtaaaagct tgtaaatttg atgcaataat atttgaagac aaaatagcta
     7921 agatagaccc taataagtgt gtagggtgta tgcaatgtgt agcaaaatgc cctacaaaag
     7981 taatatcagg tgacataact aagaaaaaga aagttactat tgaccaagaa ctttgtgttg
     8041 gatgtacagt gtgcaaaaaa caatgtaagt ttgatgcaat agagggagaa ttaaaagaaa
     8101 aacataaggt agatgcagat aaatgtgtag gatgccattt atgtatggaa aaatgtccaa
     8161 agaaagctat taaaattttg taatatggac aaaataagga tatggaataa tttccatatc
     8221 tctaatgagt acataaaaca gaggtatgga ataatttcca tacctctatt ttatgtatat
     8281 ttattctaat ggtaataaat ataacaatag tgtaacaaaa aaggttcgac atttctgttg
     8341 aattttttac aatatagtga tagactaaat aggtacaaat tacgtacaaa tgaggtgaat
     8401 aaatgaatat aatcttggca tcagcctctc caagaaggaa agaaattcta gagaatacga
     8461 atgtaagatt tgatataatt aaaaatgaaa ttgatgagat tatattggaa ggtgaaacac
     8521 caaaacattt agttatgaga ttagcttttg aaaaaagtat gtctgtagca tctgaacata
     8581 atgaagatgt tgttattgga gctgatacag ttgttgtttt agacaatgct attttaggaa
     8641 aaccaaaaga tgagtcttgt gcaagagata tgttaaaaag actatctgga agagagcatc
     8701 aagtcataac aggtattagc ttaataaact tgtgcgaaga taaaaaggta attgactatg
     8761 taatcagcaa tgtgaaattt aaaacgttat cagagcagga tattgaggat tatctgaaaa
     8821 ctaatgagtc atttgacaaa gcaggagcct atggtataca gggttatgga gctttgttag
     8881 ttgaagaaat tagaggagat tattttaata tagttggtct tccaatttca aggttaggcg
     8941 acctattaaa aaaatatttt agtataaatc ttttttatgg agtgtgattt atctgaaaaa
     9001 gtcatttaat tcaaccatta aggtaaaaga aatggcacca gaggaaagac ccagagaaaa
     9061 gatgcttgcc aaaggcgtaa aaagcttatc aaatgctgag ttgttggcta tacttttaag
     9121 aactggcaac aagaataaaa atgctattga attagccaac tatataataa atagagatat
     9181 tcagggtatt agacatttag aagatatgac aatagaggag ctgtgtaata tagatggaat
     9241 tgggttatca aaatcaaccc aaattaaagc agctttagaa ctaggctcta gagtagctag
     9301 ttttaaacct ataaagtata aaataaggaa tccttgggat atacagaggt attatatgga
     9361 tagcctaagg tatttaaaaa aagaggtttt taaagcagtt cttttaaaca caaaaaatga
     9421 gataatatct gatgtagatg tatctatagg gactttaagc tcgtcattag tccatccaag
     9481 agaggttttt aaagaagcta taagaagaag tgcaagtaag ataatagtga tgcataatca
     9541 cccctcagga agtgtagaac catcaagaga agataaaaat atcacatcaa gacttattaa
     9601 atgtggagaa ataattggaa tagaaataat agaccatata attattggag atggattata
     9661 ctttagcttt aaagaaaata tgataatttg actttatatg tgataaaata ataggagaat
     9721 gttttaaaca tgattcttaa tagtaggaag gagtaaaatc atggctaagg aaaaaaagaa
     9781 agaaaaaaaa ggttttttta gttttaataa gatgacaaag gatatgggaa tagatttagg
     9841 gactgcaaat acattagttt acataaaagg acaaggtatt gttgttagag aaccatcagt
     9901 tgtcgcaata agagatgata gtaaagaggt attagcagta ggtgaagaag ccaaaaaaat
     9961 gataggtaga acaccaggaa atatagtagc tataagacct atgaaagatg gagtaatagc
    10021 tgattttgac ataactcaat caatgattag ctattttata caaaaagcgg ctgataaaaa
    10081 aggtgttgta agtccaagaa tagctatatg tgttcctttt ggagttacag aagtagaaaa
    10141 gagagctata gaagaggctg caagacaagc tggagctaaa gatgcattcc ttatagaaga
    10201 gccaatggca gcggctatag gggctggatt aaaagttgaa gaaccagaag gtaatatggt
    10261 agttgatatt ggtggaggta cttcagaaat agctgtaata tccttaggag gaatcgttac
    10321 tgctaaatct ataagaatag gtggagacga atttgacgag tctatagtgg cttatgtaaa
    10381 aaaagaatat aatcttatga taggtgaaag aactgctgaa aatgtaaaaa ttaacatagg
    10441 ttctacattt aaagatgatg aagaaataaa tatgcaaata agaggtagag atttaatctc
    10501 aggattacct aaaacaatag aaatatgctc tacagaagtt agagaagcat taaaagaacc
    10561 tgtaagttca atagtagatg caataaaatc tacactagaa agaactccac cagaattagc
    10621 ctcagatata atggaaaacg gaataatgct aacaggagga ggcgctttgc ttagaggtct
    10681 tgataagctt attacacagg aaactggtat gtctgtacaa atagcagaaa cacctcttga
    10741 ttgtgttgca cttggaacag gtaaatctgt tgaagaccaa gaaatctttg aaaaagtatt
    10801 aatgatgaat actaagaatt agaagttggt gatggccttg agatttgaca agaacaaaaa
    10861 tgagaaaaaa aggattaatg ttaaagtgat agcaacgggc gtagttgcta tcactttaat
    10921 tggaattgtg ggtatatcaa ttggtaaatt ttctagtggt agtccagtaa atttaggagt
    10981 agcttcagat gctattacaa gtgtaggtaa gggaataaat gatggatttt cttttatcaa
    11041 aaatggtttt aaagatgtag caaattttaa agataattct aaaaaggtaa aaaaattgga
    11101 agaagaaaat gaaaaactta aaaaagatat gatagcttta aatgccaaac tagataaaac
    11161 agaatcgcta gaagagttaa aaaagacact gaactttgta caagaagagt ataaggcgac
    11221 aagtatttca gcaagtgttg ttggtaaaaa tgatggaaac tggtatgaga gttttgtcat
    11281 aggtgcagga aaaaattcag gtgtaaaaaa agaaagtata gttatgaatg gtagtgggtt
    11341 agttggcata gtttatgaag tttccaacaa ttatagtaag gcaatttcac tattggattc
    11401 taaggcatct gtaagcttta aactagcaaa ggatgccaat gctaaaggta caattactca
    11461 aaatactact ttagataata aagatagtta taatagtaaa ggatatttgc aaggatatat
    11521 gtttgactca tcatataatg taattcaagg agatataata acaacatcag gtcttggatt
    11581 cttcccagat ggtataccaa ttggcgaagt tgaaaaggtg gttgatgata aagataagtc
    11641 attaaaatat gtagttgtaa aaccttatgt tgattttaag aatataaatg acgttgtagt
    11701 catagaacca agaaatatag gataaagggg ataaaaacaa tgaaaaaagt tttactttgt
    11761 ctgttgggga ttgtgcttat gactatagaa aatagtataa caaattatat agatattttt
    11821 ggcataagct ttaatttagt tcttatatat gtaaccataa tctctcttta tttagatgaa
    11881 ttagaaggtg ggattatagc agcaataata gggcttatta aagacataac cgttggtgga
    11941 atttttggtg taaatgcatt aattttgttt attattgcat atgcaattgg atacatgaga
    12001 gatcagctat ataaaggtag ttatattatt actttcgttt taatttttat aggcagttta
    12061 attgattcaa ttgtcaatat aggtacatct agtataattt atcaaagcta caacatatca
    12121 acactttttg taaaaggtct actagtagca ccaatagtga atagtttaat tggtttagta
    12181 atatatcgct tatctaaaag agcagtttta aaacttaaag aagattagtt ggtgatttca
    12241 tggaagaaaa taaacaagtt gatagactaa aaataattaa gatagtcatg agcgtgatat
    12301 ttttagctat tttagtaaaa ataatctata tgactacatt taaatatgaa tattacaatg
    12361 agttagctga gaataaaaca tataaaaagt tggcaataga agctccaaga ggagaaataa
    12421 aagatagata tggaagactg ttggcaggaa ataaaaactt atttactgtt caagtttcag
    12481 gtaacgatat taataagaaa gatgcaaata agcattcaag agcaaatgaa atatcattaa
    12541 aacttataaa tttgctggag agaaatggtg aagaatatgt agatgagttt cctatatatg
    12601 tagaaaatgg aaagtactat tatacatatg acagagatat tagggaatat aaatcagaaa
    12661 atggtattcc aaatgattat aatgctaagg aaagttttta ttacttagta gacaaactta
    12721 tatcagctgg aatattatct caagaagata aaagattgga tgcgacaaga ttacaggcaa
    12781 aactaaatga aaatggatat tatccaccta ttttagttag taaatggatg ttcacagcag
    12841 aaagagataa gagagattgg ttggctagtt ataagataaa agaaactaaa ctgagtgcca
    12901 aagaagcgtt tgaaaaagta agaaatagtg atgcattaga aattgataaa agtttgagtg
    12961 atgaagatgc aagaaaaata atggtcgtaa gggatttgat aaaatcaaaa ggatattctc
    13021 aatataatcc agtaactatt gctaaggatg taggagaaac tacgattgct caaattgaag
    13081 aaagtgctat ggaccttgtt ggtgtttcaa tagcagtaga accagtaaga tattatccta
    13141 atgggtcatt agcttctcat atgttaggat atgtaggaaa aatgccatcc actcaaatag
    13201 aatcatacct tcaaaaaggt tatgaaactg gagatatggt aggtcttgca ggagtagaaa
    13261 agagtaatga aagtagatta agaggaactg atggttataa aatggttaaa gttgatgcat
    13321 tgggtagaat cagtaaagaa attgaatcta aaaaacctaa atcaggagat actgtgtatc
    13381 ttacactaga taaagattta caagaagttt cagataatgc attaaaacag ataatcgaag
    13441 ttgcttctaa aggaggtact tttaaaagta aatttgggga caaaccaatt agtgcatatg
    13501 caggaaaagc acagtcagca gctcttatag ctattgatgt taaaaatgga gaagtattag
    13561 cttcatcaag ttatcctaat tatgacccaa ataaatttgc aaaaggtatt tctacagagg
    13621 attataaagc acttcaacca aaaaatccaa atgacttact agcaggaagt ccacttttaa
    13681 acttggttac acaaggggaa tttcagccag gttcttcatt taagatgcta acttcaatgg
    13741 cagcattaga aaatggtttg gatccaaact ttactataaa tgaccctggg gttataatgt
    13801 taggtaaaaa atcttttggt gactatgtat ggaaccatgg tagaggaaac catggtatga
    13861 caaacttata taaagcgata caagagtctt gtaatatata tatggcaaca attggtacag
    13921 gtaaaacatg gccagatgga aaaagcattg gtatagatat gaatgcaaat aaaatacttg
    13981 agtatgcaaa attatttggg cttgaccaaa atacagggct tcaagatgaa gtagaagaaa
    14041 gagcaggtaa agttcctagc acagaagata aattgaaatc aacacaagct cttctaaagt
    14101 caaatttaga aagagaaatg gcaaatgatt ttgttgatat aactagagaa aaaaatccta
    14161 aagaatatga gaaaagaatc aatgagattg taagctgggc agcagaaaag aaaactcctg
    14221 gtagagtcga aacaatgaat aggctaaaga aaatgaatgt taaagaagat agaatagaag
    14281 aagtagcaga tttagcagta ttcagttatt ttaactttgc aaaatggagt actgcagata
    14341 catttaactt agctataggt caaggtgaaa atgcttatac accagctcaa atatcaagat
    14401 atgtagctgc tatagcaaat ggaggtaatt tagtagaatt atctgtagtt gatagagctg
    14461 tttcaagtga ttattcatca gttaaaatta atgaccagaa gaaagtcgaa aaaatacctt
    14521 ttaaaaatcc agataactta aaagaattaa caaaaggtat gaaattggtt gcaagacaag
    14581 gtacagcgaa gagtgctttt gctgattttc caatagatgt agcagcaaaa actggtacag
    14641 ctgagaaaag tgggaaaata cctacagata acgagtatga atatttaaaa agtcatatgt
    14701 catcttataa tgtcaaccta aatgatgcga ttaaactagc tgataagatg aaagcagaaa
    14761 aagaaaaaga gctttcatta gctaaggaaa aggaaataaa aaagaaatta gaaaataaag
    14821 atttgaaaga cgaagaaaga aagaaattag aggaagaatt agaagatgga gtaaaagtta
    14881 gattagagga cactgataag gtgaattctt catatctaag aaaagctata aaagaattaa
    14941 atccaaaaat aacagatgac caaatagata gatttaaaca agattatggt tcttttacat
    15001 ggactgttgc ttttgctcca gcagatgacc cagaaatagc tgttgtatgt gtaattcctc
    15061 aaggtgattc aagtgtattc tcacttttac caactagaga agttattgga acttatatgg
    15121 gattagagcc aactaatagt aaaaatgaca acaagacgaa tgatgttaac aattcttctg
    15181 acgaaaatat aaatttcgag tcacaaatta atagatagct taatctatgt ataaacttta
    15241 gctttatata gaaaaaataa aagggtttaa agcgtatttg aagaatataa atgatatatc
    15301 actctggagg ttgttatgtc tttaagagaa atttgtagtc aagaactagt agagttcaag
    15361 ggtaataaaa gagggataat agtaaatata aaaagagaag ctccatttga ggaaatacag
    15421 gaaaaaataa taaacaaatt ggaagcatat gttggctttt ttaatggggc taaaattagt
    15481 aaaattaata gtgattgttt aactgacatg gagattctag aacttaaaga aggaattact
    15541 tctagatttg atgtggaatt tgttgaagat caaaaaattg aagaaaatag taattttcca
    15601 acaaaatatg tcaataccct aagatctggt gaaaatattg aatttgaagg tgatgtagtt
    15661 atattgaatg atatgaaacc tggttcaaaa gtcctttcaa aatcaaatac agttgttatg
    15721 ggagatataa atgctggtgc taaggttgta gcaggaggta atgtttttgt aatgggtaaa
    15781 atagaaggtt ttgtacatgc aggtgcagaa ggcaatgaat ttgcatatgt tgttgctgga
    15841 aatcttaatc ctaaaatatt acaaatagca gataatattg cagaagctcc agatgatgaa
    15901 gaaaattatg aaaatgaatc agaaattagc ccagaaatag cctttgtaag taatggaaga
    15961 attgtaatcg aaagctattt gtcaaagtta gacaaataga aagaaaaata gggggtagta
    16021 ttatgagcga agttatagtt ataacatctg gtaaaggtgg ggttggaaaa actactaccg
    16081 cagcaaactt aggaactgcg ctaagcctag aaaataaaaa aacagtagtt gtggatgcag
    16141 atattggact tagaaactta gatgtagtaa tgggtcttga aaatcgaata gtttatgaca
    16201 tagtagatgt agttgaagga acttgtaggc ttaagcaagc tctaataaaa gacaaaagat
    16261 ttgataatct atatttatta ccagcagcac aaactagaga taaaaatgct gtctcagttg
    16321 agcagatgat tgatctgtgt gagaaactaa aagaatcttt cgagtatata ataatagact
    16381 gtccagcagg tattgagcaa ggctttaaaa atgcagtagc tggggcagat agagctattg
    16441 tagttactaa tccagaaata tcagcagtaa gagatgctga tagaataata ggtctattag
    16501 aagcaaatga aataaaagag ataagattag ttataaatag aattagaaat gatatggtta
    16561 agcgtggaga catgatggat aaacaagata taatagaaat attagcaata gatttgttag
    16621 gtcttgttcc tgatgatgaa agcataatta tatcaacaaa taaaggagag ccagctatac
    16681 ttgattctaa gtcacttgct ggtcaagcat acaaaaatat cgcaaaaagg atactaaatg
    16741 aagaagttcc tctacttgat cttgaagttg aagatggatt ctttggtaga cttaaaaaaa
    16801 tgtttagcat ggctaagtag caaaggagga tacatctgtg ttagatttat ttagagtttt
    16861 ttctaacgag gctaaaacta gtaaatctgt tgctaaagag aggttaaagc tagttttagt
    16921 tcatgataga gtagattgtt caccacaact tttagagatg ataaaaacag acattttaaa
    16981 agtaatagct aattatgcag aaatagaaga tgatggtctt gaaataaaaa tgtctaaatg
    17041 cagaggtgag catgatgaca aacctgtatc tgcattagtt gctaatatac cactaaagaa
    17101 tataaaagat agatgtatgt aattagtgct taaaaggggg atatccttat tggtatatgc
    17161 cccttttatt taattatatg tttaaagata gaaagacggt gattctttga aaaaaatcaa
    17221 tattaattat aagtcgacaa ttaagttgat aaaacagtta gactggaaac ttatagttac
    17281 agtattagct atctttattt ttgggcttgt tatacttagt agtgcaacac atgctaattc
    17341 aacaggttca tataatcaat tgattaaaca aggattagct tttgtacttg gtataggaat
    17401 gattatagtc atattatttt ttgactataa tcttctaggc aggtattata aagcgcttta
    17461 tataataagt cttattttgt tagccatagt tttattacct ggaattggga cagtaaaagg
    17521 tggagctagg tcgtggatta acttaggacc acttgacttg cagacatctg aaatagtcaa
    17581 attaacattt gtactaagtt atgcaaagat acttgaatcc aaaaaggata aattaaatac
    17641 gttaaaggaa gttatgcctg ttgtagttta ttctcttcca tttatcggat tattgattgc
    17701 acaaccagac ttgggaacag gaattgtatt ttgttgtatg atatttgcaa tgctgttcac
    17761 agcaggtctt agctcaaagt tgataaaaag aggaataatc atactattag tgtcaatgcc
    17821 actgatgtat ttaatgatgg cagaccatca aaaggtaaga atagaagctt ttctaaatcc
    17881 agaagatgta actctaaaag gaaactatca agttatgcaa tctctgattg caattggttc
    17941 tgggggcgta actggtaaag gactttacaa tggaagtcaa aatcaagaag attttttacc
    18001 tgtacaagat agtgatttta tatttgctgt tgtaggtgaa gaacttggtg taataggaat
    18061 ggcagtgttg ataatcttat ttatgatatt cctattaagg cttttagcaa ttgcaagaga
    18121 tgcaaaagat ttttatggaa ccctcatagt ggttggtgtt atgggaatgt ttggttacca
    18181 gataatccaa aatataggta tgacggttgc gttaatacca gtaactggag taacattacc
    18241 ttttgtaagt tatggaggaa gttcgttatt gacatcttta gcaaacttag gattagtact
    18301 aaatgtgtgt atgagaagaa agaaaataaa tttctaaatt taaatacatc taaacataaa
    18361 ttgaggagat tagatatatg aatatagcat tagtagcaca tgaccaaatg aaaaacacta
    18421 tggtcggatt ttgtataggt tatgaatcaa ttttaaaaaa gtatggacta tatgcaactg
    18481 gaactacagg aaaaagaata atggatgaaa ctgaattaaa tataaataga ttagcatctg
    18541 gtcctttagg tggagaccaa caaataggtt ctttaatagt aacacaagaa atagatttag
    18601 ttattttttt aagagaccca ttgacatctc aggctcatga aacagatata caagcgttaa
    18661 taagactttg tgatgtttat catgttccaa tagctacaaa tctagcttca gcagaaatat
    18721 ttataaaagc tcttgataga ggagaactat catggagaga agttagaaaa agcaaaagtc
    18781 aacgtattta aaaaatatta atattacagt ctaaagaaac agatatataa gtgtttaatg
    18841 ccctagtttt tctgttgcaa taaacctagt acctgtagaa atcttatgaa agatttaaat
    18901 gatgaggagc taaatttgag attaaataat taggaaatag attatagata gagtagtaga
    18961 atttaaacta ttactctatt ttaataatat gaaatagaat taagtctatt attatatata
    19021 tttaaactta taggtaatac tatttcaacc aagtaaatgt tgttacgaca acattaataa
    19081 aacaaaaatt tatttttaaa atttgataaa tagctgtata ttagtattta atctataaat
    19141 actaatatac agctatttat tatttttaag tctattgtag aatataaacc ttgtattata
    19201 gagtactatg tattattata gtaaatataa gtactataca ttatagagta gtaggtgaaa
    19261 aggcggtggt acataatgca gttaaataaa gaagtgttaa aaggttatat cgatatatta
    19321 attgtttcta tattagaaaa gaaagattgt tatggatatg aaattgcaaa gcaagtaaga
    19381 gaaagaagtg agtttgaatt aaaagaagga acaatgtatc ttgcacttaa aagaatggaa
    19441 tctaaaaatt taattaaatc ttattatagc aatgaacaaa gttcaggtgg aagaagaaaa
    19501 tattataatt taactaatga aggtaaagat tttttagaaa taaagaaaca agaatggaga
    19561 tttattaaaa aggttatgaa tcaatttttg ggagaggtgg attaatttat gaaactaata
    19621 gatgaatatt tagataaact atataaaaaa tgtgacaata aaagtactat agaattaaaa
    19681 caggaaatga gatgccatct tatagaatct gcaaatgaat ttaaattaga aggattagat
    19741 gaagaagaag catgtaaaaa agcaattgaa agatttgatg atggtgatga aatgcagtat
    19801 gaattatgta atataataaa agaactatct ttatcattgg ataggcataa atcaattgta
    19861 atggggttta aaaaagtatt aggttacata agtataattg catttttaat ttctggattt
    19921 atgtggtatt acaataatag tttacaacat aatatgtata atttaggaaa agaacttgat
    19981 ggagaaataa agcaattggc agaaagacat gatatgacaa atattggtga atataagtta
    20041 gaacttgaaa aaatacttga taaagataag tatagtaaag tcaaagctct tagactatat
    20101 gtaatagata tgaaagatgg aaacactaat ttaagttctt ctggattgaa tgctaatatg
    20161 gtatatgaga gggaagcaga ttataataac ataagtaatt ttatacagca tttaggatat
    20221 aatggaaaag attttttaga caagaatggg aatatagtaa atccagatat tttcttagaa
    20281 tatttctttt attttgaatc ggagatgtta attccagtag catttgcatt tggattatta
    20341 tgtataatag cgtattttat attaagattt aaaatttctt taataaaaaa taataattaa
    20401 ataattcacc aaataatttt aatttgtaga gtaaataaga tgtttcaaaa taaaatttga
    20461 atcatcttat tttttattgt ttgttatact cttttctttt atatttctcc atagtggatt
    20521 gtatctatac ataatcacat attttgatat aattatacct tccaaaaaat gtattgaata
    20581 tacaaattat aatctgtata atgttgttag gggagaatgt atgaagtgta tacaatttta
    20641 attttaaatg tattattgac aattctcctc actctcgtca ttaccaataa ttagaaaatt
    20701 gacaaacctg ttacagacca acatttttga atatattaat tattatcatt ttattgtgat
    20761 gatatcaaaa atgcaggttt gtgagttgga ttgtgtacga actatatagg aaatatagaa
    20821 cagattttcc aagaaatcta aatattctat gaccacgtga attgctatgc acacatgttg
    20881 ataatccata ttttaaatct aaaatgaact taattaatgg tagggaattg aaagtaaaat
    20941 atattataaa ggagaatgct aatatgaaaa aaatcaggaa attagctact aaactattga
    21001 ttgcaacgat ggttttaacc tcatgtatga gtgtaacagt ttatggtatg acagctaaag
    21061 atgttactgc aaagacaccg aaaagctatg tagctggaac caacagtata tatggtccaa
    21121 agttatcaca agctcaactt aattcggtag cacaagcaac agcagacttt atgaacaaga
    21181 aaattacaaa gaacatgacc acagatgcta agattagggt tgcttataat cacattaaaa
    21241 acaacacaac atatgttaac tggaacgcag cagagggagc caatactgct tatacattgg
    21301 ttactaaaaa aggtgcttgt tctggaatgt gtaggagctt gaaagcttta tgtgatgcta
    21361 tgggaattga atgttattat gtacatagta caacaaatga ccatcagtgg aatcttattc
    21421 gttttgagga tggacgtttg tatcatgtag atattgatgc caacaaatca gcaggagcag
    21481 acatcatata caagagtctt actcacccat taccatttga caagaaagcc tatccagctg
    21541 ttggtgaatc agtaaagaca cagaatccaa cgccaactgt taagaaacct acgccagcta
    21601 agccttctgc gccagttgga cctgggaatc acactatgat agcagatcaa gaagaagcaa
    21661 tacttgatga agtttatgga aataatctac gtcccaaaat tgaaaggtat gagggagttc
    21721 cgtgtagttt aatcaactca tcaataaata caattaagtt tacaagcaaa aattcaaatt
    21781 tagacccatc aaggcataca gtttctgtaa tagggtttat gaatacacca tatggtatgg
    21841 ttgcattagc tccagatggt gtacctgttg agtatccttt tgaatatgat aagccatttt
    21901 ctttagattt tactggtaaa aacctttctg cagcagaatt atcaaaaaag tgtaaaaaca
    21961 ataatgtaat tattaaaata gaaattggtg aaatagaacc taattctgga agagggcaag
    22021 taagatatgc tagatatgtg aattaatata aatatacaag tatacaaata gaaaagatta
    22081 taaataatgt accctcttgg tgagggtgca tttatcatta tattagtcat tttgggaaat
    22141 aaaaaaggag ttgaacttaa aattagatta gaaaaattaa ttttgagaca actccatttt
    22201 aatttatttt taaatataaa atcatttatt ttaaataata ttatgtgtga ttaatggcta
    22261 tttaactaat tttgcaaatt ctgcaccaaa tgccatacac tcttttaatg tctcttcact
    22321 agggaagaat tttttcttaa gagattctac aggcattttg aatgacatag atttaagtaa
    22381 agtctcagcc atagttatac cttcaccact ccaaccaaat gaaccaaata ctcctgctat
    22441 cttgccttgg ttagccattg ggtctataac agagaataaa tcccacatag gtttaaccat
    22501 agttttgttt atagttggag aacctagtaa tattactttt gacactacta atgtatcgtg
    22561 catttctgtt aatgtcatat cttctaaatc atataattca gcctttgcac cttctttatc
    22621 aagtccttct tttatctttt tagccatctc taaagtatta ctataagctg ataagtagaa
    22681 tatagaaact tgattttgat tagttgtatt aacagcttct gtagaccact caacatatct
    22741 tttaacagca gccattgggt ctttagtaag cattggacca tgagaagtca atatagtatc
    22801 aaattctatg tttagtccta ccaccttatc tacagcactt aaaacatgtt tagcaaatgg
    22861 cttaactata caatcatagt agtgtttagc tgattttaga taatcttcac tattcactac
    22921 ctctgcatca acacttgcaa agtgacaacc aaatgcatca caagttaaaa gagtcttgtc
    22981 ctcctcaatg taagtaaaca ttgtatcaac ccaatgtaag aatggagcag ttataaatct
    23041 aagatttctt ttaccgatat ttaaaatttc accatctttt attacatgac agttaaaagg
    23101 tctatttatt tgaccatcta agtatagttt agcagcttta gtacaataaa cttctatatt
    23161 aggatttata tctaataagt attttaaact acctgcatgg tctggttctg tgtgatgaat
    23221 tacaacatag tcaattttag ctatatcagt aacctctgat aaattactta aaaactcatc
    23281 tttaaaattt gcctttacag tgtcaaataa aaccgttttt tcgtccttga ttaaataaga
    23341 gttgtaagtt gtcccaaact cagtttccat tattatgtcg aatactttta aaccttcatc
    23401 tactacgcca gtaaagtaga tatctttttt tacttcaaaa actttactca tttttaaccc
    23461 tcctaaaatt tatcattata taagtaacat acccaatttt gaggtgaatg aaacaaaaga
    23521 tgtcatgata agtcatgtaa aacaatagtt ttatgtttat aatatatcat actttgtaaa
    23581 aaaattgtat ttcaataaaa atttaat
//
LOCUS       DAEPOG010000046        23178 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEPOG010000046 DAEPOG010000000
VERSION     DAEPOG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.046"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            183..1415
                     /locus_tag="KR432_001527"
     CDS             183..1415
                     /locus_tag="KR432_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0640071.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1429..2235
                     /locus_tag="KR432_001528"
     CDS             1429..2235
                     /locus_tag="KR432_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBH0640072.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2347..3216)
                     /locus_tag="KR432_001529"
     CDS             complement(2347..3216)
                     /locus_tag="KR432_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0640073.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3587..5014
                     /locus_tag="KR432_001530"
     CDS             3587..5014
                     /locus_tag="KR432_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBH0640074.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5090..5359)
                     /locus_tag="KR432_001531"
     CDS             complement(5090..5359)
                     /locus_tag="KR432_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640075.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5362..6804)
                     /gene="nhaC"
                     /locus_tag="KR432_001532"
     CDS             complement(5362..6804)
                     /gene="nhaC"
                     /locus_tag="KR432_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0640076.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6829..7995)
                     /locus_tag="KR432_001533"
     CDS             complement(6829..7995)
                     /locus_tag="KR432_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0640077.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(8220..10340)
                     /locus_tag="KR432_001534"
     CDS             complement(8220..10340)
                     /locus_tag="KR432_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640078.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10573..10974
                     /locus_tag="KR432_001535"
     CDS             10573..10974
                     /locus_tag="KR432_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640079.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11017..11310
                     /locus_tag="KR432_001536"
     CDS             11017..11310
                     /locus_tag="KR432_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBH0640080.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11325..11765
                     /locus_tag="KR432_001537"
     CDS             11325..11765
                     /locus_tag="KR432_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBH0640081.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11857..12330)
                     /locus_tag="KR432_001538"
     CDS             complement(11857..12330)
                     /locus_tag="KR432_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0640082.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12330..13118)
                     /locus_tag="KR432_001539"
     CDS             complement(12330..13118)
                     /locus_tag="KR432_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0640083.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13446..14690
                     /locus_tag="KR432_001540"
     CDS             13446..14690
                     /locus_tag="KR432_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0640084.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15013..16110
                     /locus_tag="KR432_001541"
     CDS             15013..16110
                     /locus_tag="KR432_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0640085.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16101..16799
                     /locus_tag="KR432_001542"
     CDS             16101..16799
                     /locus_tag="KR432_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0640086.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16802..17866
                     /locus_tag="KR432_001543"
     CDS             16802..17866
                     /locus_tag="KR432_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0640087.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18049..18753
                     /locus_tag="KR432_001544"
     CDS             18049..18753
                     /locus_tag="KR432_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640088.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18746..20068
                     /locus_tag="KR432_001545"
     CDS             18746..20068
                     /locus_tag="KR432_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640089.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20255..21628
                     /locus_tag="KR432_001546"
     CDS             20255..21628
                     /locus_tag="KR432_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640090.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22001..22939)
                     /gene="pdaA"
                     /locus_tag="KR432_001547"
     CDS             complement(22001..22939)
                     /gene="pdaA"
                     /locus_tag="KR432_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0640091.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8520 a         2898 c         3679 g         8081 t
ORIGIN      
        1 aataataaat ttggactatt tatctattta taacttaaat tatatcaaat tgataacaaa
       61 ctagatatta tacttttttg tagaatagaa ttacaattat ataaagaaaa attctattaa
      121 tagtgaaaaa aaagtagaaa aaagtagaaa ttattctttt ataaagaaag ggagggataa
      181 tattgcaaga gagagaagaa ttagttttaa atccagttcc aaaagaggaa agagttggat
      241 ggttagctcc attgtttaat atgcttggtt ctaacatagc tatatctgaa ctaatggttg
      301 gaggaacttt aattttagga atgactcttt ctaatatgat tattacatca attataggga
      361 acttaatatt agttgcaatc attatgatac aaggttatat aggttacaaa gaagggttaa
      421 atacgtatgt attagctaag ggggcatttg gagagatagg aggaaagtat cttatatctc
      481 tattacttgg tataactagt tttggatggt ttggtgtaca agcaggggtt gcaggactat
      541 cagtacaaaa gatattccca agtgtaaacc taacattagt tacagttata cttggattat
      601 ttatggttgt ttttgcactg tatggcttta aagctatggc taaatttaat tacttagtta
      661 taccaccact tatgatatta atggtgtggg gagtttttaa agctttttct acttatggag
      721 ttgaagctat ttataattat actcctcaaa caactatgag tatggttgaa ggtcttaata
      781 ttgtagtagg tcttgttata gttggtgcaa ttatatcacc tgaccagtta agatatacga
      841 gacgagtaaa agatatttgg attatcagtt ttttaggtct agggattatt tcattattcc
      901 aacaagttgc agctggtgta atgtctatgg gagcacctac ttgggatata acagaagtat
      961 tggctaattt aggttttgga tgggtagcgt ttgttatttt aatacttgca tcttggtcaa
     1021 caaacgtatc aaatgcttat tctggtggat tagcattaaa aactattttt ccaaatgtaa
     1081 aaagaaatgt tttgacttta gttgcaggtc taataggtac aataatagca gctactggta
     1141 taatatttaa atttcagtca tttttatctt tcttgggaat cgcagttcct gcaatagcag
     1201 gaattatgtg gtgtgagtat tattttatac aaggaagaac ttataagcat agagaaggaa
     1261 taaattggat tgcagtaata agctggttaa taggttttgc agcgtcgtat tatagttcaa
     1321 aaataaattt cttaatacct ccgataaatg gaattgtagt gagtatggtt atttattata
     1381 tattaatgaa atgttttgga atcaaagata aataaagggg gcaattacat gggtcgtaag
     1441 gtgaaaatag gtataattca acaacacagt gtacttggag atgttaaaaa aaatatagaa
     1501 aaagcagtag agatgataga tgacttagga aaacatgggg cagatataat ttgtttacca
     1561 gaactatttg ctacaggata taatttagag tctttaggtg gagttaaaac attagaatta
     1621 ataagagaac ataataagta catagaggaa tcaatgtcag aagctgctaa aagaaataat
     1681 gtatacctga tatctccata tggaacctta gaaaaagggt ctacacatgt ttataattca
     1741 gcagttatat ttgatagaaa aggtaagatt atgggagaat actgtaaaaa tcatctgtgg
     1801 tcattagaag cagtatactt taaaggaggg gaaaaagtag aggtatatga tgctgatttt
     1861 ggtagatttg gtgtaatgat atgttatgat gcaggttttc cagaagtatc cagagaattg
     1921 acattaaaag gttctgaaat aatatttata ccatctgcat ggagaataca agatgaagat
     1981 atgtgggatt taaatgtatc tcaaagagct ttagagaaca ctgtatatac agtaggtgta
     2041 aatttagtaa gtaatgactc taatttaata ctatttggaa agagtaaaat atgcaatcct
     2101 agaggtactg taataactca attagataca tacagggaaa aatatgtatt atcagagatt
     2161 gatttagatg aaataaaaaa gtatagggaa gatataccct atttgaaaga cagaaaaagt
     2221 agctataaca tataattcta tactataata caagttcaat ataaaatgat aaaaagagga
     2281 tgtatcaata ttaagattaa atttattata ttttgatata tcctctttaa tattatcaac
     2341 tttgatttaa atatttttaa aatagttgat aagaaagttt ttgaacaaca acacagcttt
     2401 agattcatag ttttcgctaa accatgaaag atttaaatat cttttaaatt caatatcttt
     2461 gatatgtaac aacttaatct cagggtcttg agataatccc catgattttc cacatataaa
     2521 tccaactcct tgaagtgatt ttataagagc ataaattgta tatggtgaat cagactcaaa
     2581 tgctattttg ggtttgaaat tagctgattc gcacaggata tcaattacct ctctataatt
     2641 ctcacccttc gttataacta taaagttttc atctgaaact tcgcttagat atacttcatc
     2701 ctttaatgat aaaggatggt ttattgaaac tcctaatagt atttcttcgc atgttaaagt
     2761 tatgctattt tctgagttta gctttgtaaa tgaagaagat atgtacaaat caaaatcact
     2821 ttttttataa gatgatggca aagtatgtga cacattaaaa gtaatattgg gatatagttt
     2881 tctgaaatta ctcaaaagtg atggtaacac attagccgct gcaggagata atatctttac
     2941 ttctcctgaa ttttctaaag aagtgtcttg taactccttt tttgaacttt ctactaaatc
     3001 taaaatgcta tctactcttt ttagaaacaa tcttccatac tcatttaaaa caatatatct
     3061 tccttttctg tcaaataact gaactccaag ttctttttct aaggaagata tagttttgct
     3121 taaagctggc tgtgctatat ttaacttgtg tgatgctttt gttatgtgct ccattctagc
     3181 aacagtttga aaatacttaa gatgcaataa atccatagta taaaatacac ctcttttaat
     3241 atgacttgag ttatatcaat tttataatca actccacatt atacattata atataaagtt
     3301 tatataatca actacaatat attaaaaagt tatttaaatt gttatttata ttttgtacat
     3361 aatctaatta aaaaaagtag ctttaaatga ttaactaagg aaatgttgtt acaaacttta
     3421 gttatataaa aaattcattt gatgagtaaa gtattaatgt caagtaagaa taaaagctta
     3481 ttagatagaa aaataatatt ttataataat gatgttactt aaattattat gttatactat
     3541 tttaaatagg aaacatttag atactataat tttaggggga gtcaagatga aattaactaa
     3601 atcaatatca tcaatgctat gcttagctct agttttaagc ttatcaccag tagcatatgc
     3661 aaatgaaata agtagaacaa cagtggagaa tactttagta ggtcaggaca gataccaaac
     3721 tgctgttgaa gtaagcaaaa aaggatggag tagtgcgaat gaggctgtag ttgtaaatag
     3781 tgaatctatt gtagatgcat tagctgtaac accatttgct aaattaaaaa atgctcctat
     3841 attattgaca gaaaaaaata atctaaattc acaaactgag aaagaattaa aaagattagg
     3901 agttaaaaaa gtttatataa ttggtctatc tagttctgta tcaaatgatg tgcaaaaaca
     3961 gttagaatct aataatttaa ctactgatag agttggtggt agtgatagat accaaacagc
     4021 tttgagtata gctaaaaaaa tagaaggatt aaaagatata tcagaaatag ctgtagtaaa
     4081 tgggtacaca ggtcttgcag atgcagttag tatagcttct gtagctgcta ctaatggaat
     4141 ggttatactt ccagtatctg atgcatctgg tatatctagc tttaaagatt ttatagaatc
     4201 taagaatata aataaatcat acataatagg ctctactaat gctgtatcag ataaaataaa
     4261 acaaagttta cctaattcag aaagaatagg tggagcagat agaaatgaaa ctaatggtaa
     4321 agtaatagaa aagttttata catctaataa attaaacaat gtatttgtgg ctaaggatgg
     4381 aatgaaaaat caaaatcaac ttatagacgc tctagcagta ggagtactgg catcaaaaga
     4441 aaattctcct gtactaatcg taggagaaca attaagtgca acacaaaaga gtgtattttt
     4501 gaataaaaat gctacctcta taactcaagt tggtagtgga ggaaatgaaa atgcatttaa
     4561 agaattaaaa gatttacagg caaatagtaa caataagaga gtaatgtatg taactaatac
     4621 agataaggta aatataagaa atgatgctac aatagaagct tctgttatag gatatctaaa
     4681 taatggtgat gaagtagaag ttttagatgt gttaaaaact ggatgggtaa aaataaaata
     4741 caatgaaggt attggatatg ttagtgggtc ttatttgaca aataataagc ctgataatag
     4801 taatgaaaat ataaaaataa agtatgtaaa agaaaaagat ggtctaaatg ttaggaaagg
     4861 accaagtaca gaagatgaaa aaataggtca cctttcatat ggaagcaaag tagaaacaat
     4921 agaaatattt gctactggat gggtaaaaat aaagtataat ggtggatatg gttatgtaag
     4981 taatgattac ttatctgata caccagttat ttaattataa gtaacaataa aaaactgctt
     5041 taatttgtaa agcagttttt ttataaaaat ttaagtctaa taactatttc tattttgata
     5101 ccttaatcct gggatttatt attatggata ttatcaacat acaaacactc caaatccagt
     5161 gtctaattcc aaacttagat attaataaat tcagtcctgc tatggtaaat aagcagtatt
     5221 taattacaat taaaagtgta tttatagaaa tccccttcat agaaagtatc ctaaatccaa
     5281 taatagacat tctaagtcct ataaatgcca ataatatacc tgcaatatcc tgaattaagt
     5341 aatagtattc aaatttcata ttcaatcaac tcttctctga gcagtatcaa atatttctaa
     5401 ctcttcaatt tcatcatctg tgtatgtaag catagtaaaa ccagttacac cataaataaa
     5461 tgaaattata ggtgtaatcc aacttaagaa acaatatgga ataaattcta gtgggcttac
     5521 accaagagtc tgagcacaga aaacagcatt tgaattccat ggtattaatg gaccaccaag
     5581 tgtagctgta tcttctataa ttctagatag attttgtggt tttaatttaa actctttgta
     5641 tattggagac attagagttc cgacaaatac agaagtaaac atcattgatg aactaaatgc
     5701 atttgcaaag tagcttacta taaaagtaga cccaaccaat ttagttcttg attttcctat
     5761 tttagttata agaggttgaa gcagtacttc taagaatcca gttttttgga gaataccggc
     5821 aataacgaaa actacaaata ctagtaaaac ggtttcagcc atactcatta ttccaccacg
     5881 atttaatagt ttgtcggcat agacaaatcc agtatctata gtaaatcctg atagcatgta
     5941 atttaaaata tcacctattt tttctccttc ttgaaatact gctataataa gtcccatgat
     6001 agctgaactt agtatagata taatgggtgg cttttgtagc aataaaagta ataataaaaa
     6061 tattattgga atcattgcta ctaaaccaat atgaaagttt gaatttaata catccttgat
     6121 ttgatttatt tgaacatagt ctatagtatt atttgaatac ttaaatccaa ttactgtata
     6181 cagaactata catattacgt atgcaggtcc tgtggtataa aacatatgct tcatatgtgt
     6241 aattacatct gtcttacaaa ctgctgcagc taagtttgta gaatctgata gaggtgacat
     6301 cttatctcca aagaaagcac cagaaatgac tgcaccagct gttaaaccaa gaggaatacc
     6361 catactagta ccaatactca tcattgctat accagaagtt ccaagagtcc cccaagatgt
     6421 accagtagca acagaagtta atgaacatac gattaatgtt gtaagcagaa aatatttagg
     6481 ggttattatt gttagtccac tatatactac tgttggtatt gtaccagcag ctatccaagc
     6541 tgctattagt acaccaacag aaagtataat taagtttgat tgaaaggcat ctttaccaac
     6601 ttcaaatcca aaagcttcta tttcatcatt agtataacca agtttcgtgg ctgcaggaac
     6661 tactataagc catgaaagta aaaacattgt agttatagaa gcattaaata caactatacc
     6721 aactgatgtt agagctatta ttgcaagcat tactaacaag gaatatctaa atgaaggtat
     6781 ttttctttct gtattcattt tcatagtgca aattctcctt aaatattttt atttaattgc
     6841 attttttatt cttataagag cttcttcaag catacttctt ggacaagcta agtttattct
     6901 ttgataccca cttcctccaa tgccaaagct gtttccttga tttagagcaa ctttaccttt
     6961 ttgtacaagt acactttcta gttcctcatc acttaatcca agtgcactaa agtctaccca
     7021 taataaataa gtaccttctg gttttctaac tttaagtttt ggcatatttt catttatata
     7081 ttttatagca aaatctatat tagattctaa atattctaaa aatgattcta gccaagattc
     7141 accattatta tatgaagctt cagttgcaac taagctaaag caattatttc tctttatatc
     7201 aattctggta aaagcatcat ctaacaattt atagtctttt tcatcaggta gtattacata
     7261 tgatgattga agaccagcta tattaaaagt tttagttgga gccatacaag taattgtatt
     7321 tttttcaaat tctttagaaa tagatgccat aggaatatgc ttatgttttt ttaatataat
     7381 gtcactatgt atttcatcag atatgatttt tacattatgc ttaagacaaa tatctcctag
     7441 tttttttaat tcatcttttg tccatactct accaactgga ttatgaggat tacaaagtat
     7501 gaataatttt acatctttaa tcttgttttc aatgtcttca taatccatta tatagtttcc
     7561 attttctagt ttctgtaatg ggcttataat tagttctcta ttgttatttt ttacaacact
     7621 attaaaaggg ctgtaaactg gttcttgtat cattatttta tcatttgctt ttgtgagttc
     7681 atttattaaa agacttatag ctgggataac acctggacta taaattaacc actcactttt
     7741 tattttccag ttatgtctcc tatctagcca gtttactata gattcattgt aagaatctgg
     7801 tcttgttgtg tagccataaa tctcttgttc tagtctattt tttaaggaat caattataca
     7861 aggtgcagcc ttaaaatcca tgtcagccac ccacatagga agtaaatcat tagttccata
     7921 ctttttttcc atttctgacc atttagatga aaaattattg cttctatcaa caatctcatt
     7981 aaaattataa ttcattgtaa atacctccag taattttaat tttatgttaa taatattaag
     8041 caaacaacgt gccaatattg ataataaaaa atatttattc acaagttaat atttaatatt
     8101 aagtaatttc agagcataga taagataaaa atattaatta tacttaaaac tttgtggaaa
     8161 aggttatttc taataaattt ttaaatatat gaaaatgtaa ttaaataggt taaataggtt
     8221 taactgttta acttgttaga cctatttata ttttttagaa attctactcc ttttgatgtt
     8281 ataattgaac ctccacgacc aacaagtatg tctacaagcc caaagtcttg tagttctaaa
     8341 agcatatttc taatctgttg ttctgtcaaa aatctatttt cctctaaagc tattttacat
     8401 aagctttttc tacctattct ttcttttaaa tcataagcct tttttaaatt atccaatata
     8461 aatatgtatt catctagtga ccttttaaaa ttatagtcgt atttcatgat gtttttgtct
     8521 ttgccaatgt tgaattgata cttttttata ttatcactta ctttattaca aacggttcta
     8581 ctataatttg aatctatagt gtccaatata tattcaggta aatcacatat ttctataata
     8641 tcttttccca agtaacagaa gtattctcct aagtttctta attctcttac atttccttcc
     8701 catctataca ttttaaagat ttcttttact tcctcactta gaataaaatt acatggaata
     8761 tcatatttta atgaaccaaa aatttcaaat atatcagcag ctctttccct aagtggatgt
     8821 attttaaggg gaagaacatt caacctaaaa tataaatctc ttctaaacat atttttagat
     8881 acaagttctt ttaaatttct attagtagct gcaattattc ttacatcaat atcaatgaca
     8941 ttattagaac caatacgtcg tacttgtttt tcttgtatga ctctaagtaa tttagattga
     9001 gaatttaaat ccatttctcc aatttcgtct aaaaatatag ttccattgtt ggctaattca
     9061 aataaaccaa ttttcccacc ttttttagca ccagtaaaag ccccttcatc atagccaaat
     9121 aattcacttt gcagtagatt ttcttgaaaa gtactacaat ttactgcgac aaagggacca
     9181 tcttttctcc tagaagcact atgaatggat tgagcaaata attctttacc agtaccactt
     9241 tcaccaatta taaggataga tgaatttgat tgagccattt tattagcaat tttttttgta
     9301 ttaatagttt gtatactaga acctaatata tcatcaaatg tgtatttact gatattacca
     9361 gagtttacta gttgtgctct taatttagct tgcttttttt ctaattgaga aaactttgtc
     9421 atctttagta taaatccatc aaatacattt aattgtatat gtttaatctc taaatttata
     9481 tcaatatgat ttattttaat caatttctca aaaaatggag ttttattggt caatatgttt
     9541 tgaaaattga tgtcttttat acaatcacca acaaatgagt caatcatttc atttgcattt
     9601 atagacaaaa tttttctagc tatatgatta tagaattgaa ttattccatc attatttgtg
     9661 catataattc catcatcaat tatagatagt aacaaatcaa attgtctttc aaacttattt
     9721 gcatctaaca ataactcacc tgtactataa cttgtaggaa ttattttttc tacgtatttt
     9781 tttattaggt catcttttat tagatgtttt aatttggttt ttgtagcaat ttcaacaata
     9841 caacttaaat ctattattct gtattttaaa tctacaactt cacaattttt aaacttaggc
     9901 aggatttttt cgccaggagt taggattaca gcatctgtgg gagtaaactc aatctctgga
     9961 tagcagggca ggatatttat attgtctatt cctaatctat gtattaaaga aattgtttct
    10021 attgccatat ctttacttaa gttatataca taaataattt tatctttttc taatttggat
    10081 atcatatcaa tactgttctt ttcaaatgtc aacttgggaa caatgatttg agcatcatag
    10141 tgagataatt ttactatttc atcgtatttt aaataagcag ataggagtat aagtttttct
    10201 ttaaaaaact ttaaatttcc ctcttcaaat gagtataaat ttatctttag attatcacct
    10261 aagaattgcc ttatttgatt ttcataaatt tctccagcgt ctttttttaa acttacaata
    10321 gctaattcca tttgtttcat tttaacctct catacaaatt tgacttattt tagattacat
    10381 aataattaaa ttagattaaa taatattatt taattattat tataaattta ttatagacta
    10441 cacattgaag taaaagaagt agctaaaatt attcctttta tttttgtttt ttgtatctat
    10501 ttggtataat agtattagag cgtataataa tttaggcaaa attttttaag gaagactaga
    10561 ggagaatata aaatgaaaaa atgtctttac tgtaataggg atataaagtc accaaataat
    10621 aaattagcta taaaatatga tgacaatatg aatatatatc catgtcgtgc agaatgtaag
    10681 gaaaaaattg aagaatatat agcaaaaaaa cctacatata agtttataaa tatattagag
    10741 gttcttttag gtgtgggcgg aatattctgc tttttgagta aatggactat tgcagcccaa
    10801 gttgtacttg gaattgatgc actggttata tttttattcc cattggcagg atttaagatg
    10861 aatggaaagt taagtatgga acaaactaag aatcaaagta ggtctattgc aatatcaatt
    10921 ttggcattta tagtaataat aaccgtatta tattttaaac aagttggtaa gtagagcata
    10981 attgtataag acatcattag agtatgaggt gagtttatgg aagaaagaaa attagcacca
    11041 aacaatgaga gggaagctct cataaaaaga ttaaaaagaa tagaaggtca agttaagggg
    11101 atacaaaaga tggtggaaca agaacgttat tgtgttgata tattggttca aatatctgct
    11161 ataaggtctg ctataaatag agtaggcaca attatcttag agaatcatat aaaaggatgt
    11221 gtagcagaaa gcattaaaca tgatgatgct gataaaactg aagagatgat aaaagagctt
    11281 atggatacta taaataaatt tacaaaataa ggaggcggtt agttatgaat caacaaggat
    11341 ttattatgga aattgttgat gatagaactg caaagttaaa aatgcaaaga cattcagctt
    11401 gtgctgcttg tggaaaatgt gctactacaa attcagcaga gtcaaaagaa atattagtag
    11461 aagtagacaa cacaattggt gcaaaagttg gtgaccatgt agaagtgagt atggataata
    11521 tgaatgtatt aaaagcagct gtaatggctt atataatacc tcttatagca cttcttgtag
    11581 gaacaatagg gacttattat atattggggg caattggaat gacaactgga gttgaagcca
    11641 taagtggatt agttggaatt tgtcttacaa taatctgtta tttatactta aagaagaatg
    11701 acaaaaaatt tagagacagt agagaattta taccagtaat aacaagaata ttaattgacc
    11761 tttaagataa ttataattaa aaaatagcaa tgtgcttttt aaataaatac tcaatacgta
    11821 tttgtagagt ttaagtacat tgctattttt atatttttat tttgattttc tgtaaggaat
    11881 aaagtttttt ctgattttca aaagattcaa tgttcttaca aaagaagtta caactaatac
    11941 accaagtgct attgcagcac ttagaaatag cgcatctgta ccttttgaca tagccaaaga
    12001 ataatttttc tgaatcaaag acaacattgt attatatagc ccaattcctg gtattagagg
    12061 aagaatacct ggaattacga acacaatagc tggttgcttt agttttcttg ctaaagtctc
    12121 actacacgct gagaccaaag cagcagcaat aaaacctgca aaaactgcat ttgtagtaaa
    12181 attaaataaa taaacatata caatccaacc aattccgcca gttactccag ctggaagaag
    12241 tagatatagt ggagcattaa agaaaacagc aaatccagca gttgcaacac ctgaaaataa
    12301 aaaatgcaaa atcatagaca tatcagtcat tagaaaggac ctcctattct ataccatgta
    12361 tcaaggacaa aaccaactcc agaagcaatt gcaataataa tcatagcagc atcaaaagca
    12421 cgagaaattc ctgatattaa attccctgat attaaatctc taatagcttt tataaaagca
    12481 actcctggga gtagtggcat tatagcgccg actataagca ttttagggtc atctaaaatg
    12541 cctatttcaa ctagtagaac tcctgaaagt gcaattaaaa aagaagatac taaacaggaa
    12601 aatgcagata ttgcacttat cttcatcgtt ttatcataag taattgcagc aaagattgct
    12661 attataaacg taagtataaa atttaataca taattccctc caagcaaaca agcaaaacaa
    12721 gcagatgcaa ttcctgtaca agagtaaaat aaccataaag gataaggttt tacatcttgg
    12781 atttcataaa gacgtgcaat ggcattatca atatcatact ctttgtcact aacaaattct
    12841 cgagaaaaac tgtttaataa agaaatcttg tttaagttta tacctcttga ttgaatcgtt
    12901 ttcataaacg aaaagccatc aaatttctca tctgaaataa ttattactgt aggagttgta
    12961 aaaatattta catgataaaa ccctcttgac ctgcatattc taaggacact gtcttcaacc
    13021 ctagatgttt cagctccatt ttttatcatc agttccccta caaatagagc tagttttaga
    13081 atatttttct tatattcaag ttcctcattt tgattcataa atacacctcc aaattaaaaa
    13141 acgtaatatt gaaattatat caaattttta agtaatattc cttaatatct atataaatat
    13201 attatctttt ttttgtttca aaatagtcac atttaaatta tatccaatta aaagaattag
    13261 acaatattat ttattatatt ttaacttaaa aataaagaaa gtaactatgt taaatatgga
    13321 aaaatggcaa aacacttgac tattaattgg taaaggacta taataataaa ttatcatatt
    13381 gataaaaacg aaaattatga aatgtctgta aaaataaaat ttaaataata actaggagga
    13441 gtaatatgtt tgagaacttg aaaaaagcgg atcctgagat ttatgaaagt atgaaaagag
    13501 agttaaaaag acaacaaaga aatattgaat taatagcatc tgaaaacata gtatcagttc
    13561 cagtaatgga aactatggga agtcatctta caaataagta tgcagaaggt tactcaggca
    13621 aaagatatta tggtggatgt gaatacatag atgaagttga aactcttgca atagatagaa
    13681 tcaaaaaaat atttggagca gaacatgcta atgttcaacc tcactctgga gcaaatgcaa
    13741 atattggtgt atattttgct atgttaaaac caggtgatgt tgttatggga atgaatttat
    13801 ctcaaggtgg acatttaact catggtgctc cagttaatat ttctggtcaa tattataaat
    13861 tttatgaata tggtatagat aaagacagtg gacttataga ttttgatgaa gttagaaaga
    13921 tagcacatga agtaaaacct aaaatgatag ttgcaggagc aagtgcatat cctagagaaa
    13981 tagattttaa aaagtttaga gagatagctg atgaggttgg agcattgctt atggtagata
    14041 tggctcatat agcaggtctt gtggctgctg gtattcacca aaatccatgt gaagtggctg
    14101 attttgttac aacaacaact cacaaaactt taagaggacc tcgtgggggt gttattcttt
    14161 gtaaagaaaa atatgctaaa gacatagaca aagcaatatt ccctggtatt caaggtggac
    14221 ctcttgaaca tataattgct tctaaggcag tttgctttaa agaagcttta agtgatgagt
    14281 ttaaagaata tcaggtacaa gttgctaaaa atgcaaaagc cttagctgaa gaacttatta
    14341 aaagagattt taaattgatt tctggaggta cagataatca tttaatacta ttagatttga
    14401 ttaataaaaa tataactggt aaagcagcag aaaagagatt ggatgatgct tatataacag
    14461 ctaataaaaa tacaattcca tttgatccaa atggtgctct tgttacaagt ggaataagac
    14521 ttggtactcc agctgtaact actagaggta tgaaagaaga agatatggca ataatcgctg
    14581 aggctataga cctatgttta acttatgatg aagaatctaa ggctagaaca ttggttgttg
    14641 gtttaactga aaaatatcct ttatatgaag aatataatat tatggattaa ttataaagaa
    14701 caactaaata aaatacattt aaataattac aatgattgtg tatgtatttg aatatattta
    14761 gatataaaaa aggtgtctca aaatgaactt tttagtttat gaggcacttt tttgtatgga
    14821 atcgaacata tttgtgttat ttaaataata aaaaatatgg ttatctctat ttttagatgg
    14881 tctctttgta ttttttaatt aaaactttaa gatttcttaa gaattatact aggtttttct
    14941 aaagatttaa agtctatact tataagtata gtaatcatat gaaaagaaaa gctttaacga
    15001 ggagggaatc ttatgaaggt attaattctt acaggaaaat ttggtatggg tcattactca
    15061 gcatcaaatt ctttaagtga agatataaaa gcaaaatttg ataattcaga aataacaatt
    15121 aaagatatat ttgaatacat tatgcctaac tattcagata aaatgtacaa gactttttct
    15181 attcttgtaa atcggggaag tagtctttat aatttgtttt ataaatgtgc tgaaaatgga
    15241 aagaaagata taaaatttac tttttcagat tattttttaa ataaattaga tacattactt
    15301 catgaagtac aaccaacagt agttatttca acttttccat tttgttctca attagtatca
    15361 agatataagg aaaaatataa ctcaaatctt cctttaataa catgcataac tgatataagt
    15421 agtcactcag aatggattag taaaaataca gactgttatt tagtagctag taaatctact
    15481 aaggaagaac ttgtttttaa aggaatagat gaatctaaga ttaaagttaa tggtattcca
    15541 gtaaaaaaag aatttaaaag aatagaacat gtaaatcatt caactaaaaa aaatattctt
    15601 ataatgggtg gaggattagg attactgcct aaatcagaac aattttacaa agaattaaat
    15661 agtttagaag gagtaaaaac tactgtaata actggtaata ataaaaaaat gtactataaa
    15721 ctatatggta gatatgaaaa tatagaggta gtaggttaca ccaatgaagt atataaatat
    15781 atgaaagatt ctgaccttat tatttctaaa ccaggtggaa taacactatt tgaaacaatt
    15841 tattcagaac ttccaatatt ggcatttaat ccatttttac aacaagagat agataatgct
    15901 agttttatat taaataatga aattgggaga atacttggta aaaataaaaa atactatgta
    15961 gatgagataa aagatttaat ttatgatgat gctactctta aagaaatgag cagtaacatg
    16021 aaagaattaa aaaaacaatt tgacaataat accttagaga atattttatt ttcacttgat
    16081 gaacaaggag cgtgtagaga atgtatgtag taggtttaat tatcgttgtt gcattgattt
    16141 ttttagtcca ttcaattata cctacatact ataataaact attaaataaa gaggtattaa
    16201 aaaatatggc tggagaaaat gaaattgcac ttacatttga tgatggtcca gataagagat
    16261 atacagaaaa attactagat gtgttaaaag aaaatgatat acaagctatg ttttttgtag
    16321 tagcaaagaa tgctgaaaaa gagcctgaaa ttataaaaag aatgttgaga gaaaatcata
    16381 tagttggttt acattcattg gaacacagaa atgcatggct ttatagttat agttatgtta
    16441 aaaaagattt tattgaaagt acaaatataa tgaaaaattt aggtgtagat gtaaactact
    16501 atcgtccacc atggggtcat actaatatat tttcaaattc atttgttaaa aaatataatc
    16561 taaaaatgac tttgtgggat gtaatggcag aagattggga gaaagatagt acagtagaca
    16621 ttattataaa caagttaatg agtagaacaa aagaaaattc tataatttgt ttacatgatg
    16681 caggtgaaaa ttcaggtggt gcagtaggtg cacctgagcg tactattgaa gctttaaaga
    16741 tagctattcc aaaacttaaa gccagtggtt taaagttcgt aacaccagaa aggatgtagt
    16801 tatgataagt aaaactgaaa agaaaaataa atgcctaggt agtgtagctt ttttagtttt
    16861 actaatgggc ataacaggat attttgtttt tagagggcag tcagttgaat cattgataaa
    16921 atcattaaaa ggtgcaagcc ctatgtttat actaattgga tttgcaatga tgtttatata
    16981 tgttgcctgt gaaggtataa atatatactt aggtatgaaa gctttaaacc aaaaaactac
    17041 tcttttaaaa tgtatgggat atgcttttat tggattttat tttagttcta ttacgccttc
    17101 agcatcaggt ggtcaaccag cacaagttta ttatatgaaa aaagacgata taaatatatc
    17161 atattcttca cttatactat tagtcattgt tgttattcac caagtagtta tactggcata
    17221 tagtggaata atgttcatta tggaacgtga gtttatatta aataatgtaa gtggtatgaa
    17281 cattttacta atatatggtg taataacaaa tgttgctctt gtcattggag taattgcaat
    17341 tatattttca aaaaaacttg taaacaattt tataatatca ataacaaatt tattaggtaa
    17401 gttaagaata ataaaagatg ttgaaagttc aagaaaagtt ataatatctc aaattgaaga
    17461 gtatgttaaa ggtgctcaat atataaagca gaatccaaaa cttgtagtcc aaattttagt
    17521 cataactatt gtacaaataa cagctatgtt tttagttcct ttctttgtat acaaagcttt
    17581 tcatctatca acatatactg tatttgagat attagcgata caatccttgc ttaatattgc
    17641 tgtttcttca ttaccattgc caggggctgt tggagcttct gaaaatagtt ttatgacatt
    17701 atttaagata tttttccctg gtcacttatt agttccggca atgttactta gtaggggaat
    17761 aagtttttat gcatttgtag ctataagtgg attgatatgt atagtagtac atgtaaaatc
    17821 ttctgaaaaa gtaaaagcag tgaaaaaagt tgcttatgta agataatatt atatagatta
    17881 tttttattaa tatgtcaatt tgtgtgtaca ttttaattaa gttttaaaca tgtttacagg
    17941 agtataaact attaaaacgt gtgacattgc taagcttttg ctaataaagt ataatatttt
    18001 atatagtttg aaatatttaa gaaatatagc aaaaaaagga gcttggtgat gaataaagag
    18061 attccaaaaa aaattttagt agctgatgat aatcctgaga ttagggaaat cgtagaaata
    18121 cttttaactg gtgaaggtta tgaagttatt atggcaacga atggagaaga agctgtaaac
    18181 ttagtagata gtacgattga tttgattatt ttggatgttg taatgcctgt aaaaacagga
    18241 tttgttgcat gtaatgaaat tagagaaaaa acgacagcac caatattatt tttaactgca
    18301 aaaactcaag actctgataa agttattgga ttttcagcag gaggagatga ctatttatca
    18361 aagccttttt catattctga actaatttca agggttaagt cgctacttag acgttattat
    18421 atataccagg gaaaggaaaa ggaaagagag ccaaatatat taaaaacaaa agagttatgt
    18481 gtcaatttag atactcaaga tgttacacta aatggagaac ctgtgttttt gacaactata
    18541 gaatatagta tattaattct tatgttgaaa aatagaaaaa aagttttttc gtctgaaaat
    18601 ttatatgaaa ctatttggga tgagcagtat ttttatacgg caaataatac aataatggtt
    18661 catattagaa atttaagaaa aaagttagaa aaagaccaaa aaaatccaca atatataaaa
    18721 accgcatggg gaaaggggta ttatattgat taacaaaatt aagccatcta agttgggagt
    18781 tatactattg atatatgtag taatctcagc tgcagtggct ttcattttaa atataccatt
    18841 ttactctgct gttgaatcta tagtaaaaga tgtatcagaa gggaaagtat attttaaaag
    18901 agaagtaaat caacttatgg ataattttca aacttatgtg aataacaatg gtataagagc
    18961 agatgatgga gatgatatag caaaatggaa tagtgataat tggtttgttt ctatgaagat
    19021 atataaagga aataaggttt tttatgatac attatactat cctaatgaag tacctgataa
    19081 gagtgaggaa aatgagtttt atagcagtcc catggctaga aactatgaga tacaattttc
    19141 tgatattaaa gctaatatag ttgtagtagc tttatttaga tttagatttc aaaattttat
    19201 agaagcaatt tctattacat ttatggcact tacatttatt ataacatttt tactgttgtt
    19261 tagtagaaag ataaagtacc taaggaaaat agaaatgggt ataaagatag tagagagtgg
    19321 cagtttaaag tatagaatcc cagtaaaagg taatgatgaa ttatcatcac ttgcatctag
    19381 tattaatgag atgagtcata cattggagaa tcaacttata aaagaaaggg agatgaagaa
    19441 taaaaataaa gatatagtaa cttcgatatc acatgatgtt agaactccat taacatcagt
    19501 aatttgttat ttagatttga tcaaagacca taaatacaat agtcctgagc aattggaaca
    19561 atacataaaa aatgtaaggt ctaaagcata tcaaataaaa gatttaacag atgacctttt
    19621 tacgcatttt gttaattctg gagaggaagt ttcaaaaggg tatgaaaaaa taaatgctaa
    19681 tgaatttatt tttcaattac ttttagatag ttcatataca ttggaggaga aaggatttca
    19741 tataacaata gaaaatgaac tttataacca atatcatatt tatgtagatg ttacacaatt
    19801 tagaaggata tttgataatt tatgttctaa tataataaaa tatgctagga aagataagcc
    19861 tatagtattt ttaattaaag tagaggattc taaattacta attatacaaa aaaatagagt
    19921 aagtaatttt tctaagaatg tagaaagttt tggaattggt ataaagaatt gtgagaaaat
    19981 agttgcatat cataatggaa gcataaatgt aaattcagat gataaagaat ttgctattga
    20041 aatatctatt ccagcaaaag aatcataaaa atttaaatta tatattgtgt aattgctatt
    20101 atatattgtg taattgctta tgtaaaattc tatgaagcag gaagcatgtg actttagtta
    20161 tgggatgttc aatattgtgt acctctaggc aagtaacaag tttacttaca agaccatata
    20221 atattattat cttaaaaaaa ggggacataa taatatggtt ttgacaggag cacattatat
    20281 ttacattcta tttatggtaa ttatattaat tactatgatt atgaaaaagg atacaatagt
    20341 accatgtata ttaggtgttt tttttatggg attgttcttt gaaaaaaaca tctttggtgc
    20401 tataacagca gtgtttaact cttttattat atctttaaat gagttgggtc caattatctt
    20461 gattatagct attatggttg cattatctaa agccctagaa gccaataatg ctatacagta
    20521 tatggtgaga ccattttcga gagttataaa aaattctaat acagcatttt ttgtaactgg
    20581 atttgttatg ttagttcttt cttggttttt ctggccaact ccagctgttg ccttagttgg
    20641 agctgtattt ttacctgttg ctatgagagc tgggctacca gcaattggtg ttgcagtagc
    20701 attaaattta tttggtcatg gtcttgcctt gtctacagac tttgttattc aaggagctcc
    20761 aagtataaca gctggtgcag caggtgttgc agtatcatat gttataaatg atggtatgat
    20821 tttgttttgg gtgatgggaa tcgtaaccat atctatggca ttttatacat taaaaagaga
    20881 tattaacaaa ggaatgttta gagaagaact aaaaggtttt gaaagtgaag aagtagaaga
    20941 gttcaatgga aaatctaaaa tagcaactat tttagtagct ctgggatttc ttgctgatat
    21001 aattgttatg tatatatttg acctaaaagg aggagatgca tcagctcttt tgggtggaac
    21061 agcagtattt ttaataataa ttataaatgt tataaatttt ggcaaagata gtcttgaaaa
    21121 tgtatgtgag aatataattg atggttttgt gtttgggata aaaatatttg gagcaattat
    21181 accaatagca gcatttttct atatgggtga agttgcgccc ttgacaggtg tatttggaaa
    21241 agtattagca ccaggttcac aaggtttatt atcagatata ggcatagctc tttctcaaac
    21301 aataccactt aataaatttg ccgtatcagg tatagaaact gtagtggggg caataactgg
    21361 gcttgatggt tctggatttt ctgggatttc attagttggt tcacttgcct ctgtatttgg
    21421 aactgctata aatgcaagtg ttggtgcatt agctgcatta ggacaaatca gtggtatatg
    21481 ggtaggtgga ggctgtctag ttccttgggg tcttatttca gcagcggcaa tttgtggggt
    21541 tagtccaata gagttggcaa agcgaaactt tgtcccagtt ataaccggat tggttgtaac
    21601 aaccatagta gcaatgttta ttatttaata aatcgaataa atctttaatt gtacaaatta
    21661 aaaatagatg tgtagctata atttattata attgttttac taattttaag ccatacatct
    21721 atttttattg atatttttat tagtattctt taataaaata atatttttca ttatactata
    21781 cataacatct aaatataaca tctaaaatac tgttatctat gtttaatatc ttgccaaatt
    21841 ttttgatgtt ttattaccct gtcccaccaa catatataag ctatcgcaag ttttttacct
    21901 atactatcac caaacaaaaa ttttaatttt aatttattat tttataatct atattatatt
    21961 aactatatta gtttgtagac tttagatgta attctaaata ttaatactcc aagagttcaa
    22021 attcatatcc tagttttctt gcatttgata taaaatcatt taaaatttct gtactagttt
    22081 tggaaactgc atgtagtaac aagatagaac catcatgtag attatccatt attttttgcg
    22141 ttgcttcttc atgtgatggt tgtttatcag tatcccagtc tctgtatgca aaactccaaa
    22201 atacagtctt gtatcctaaa tttttagtca ttgccaagct tttttctgag tattttccca
    22261 taggaggtct aaaaaattta accatatctt tcccagttac atctttatat aacttttcaa
    22321 catcatataa ttcgtcatta aagtttttta gattgcttgt ttttgttggc atagatggat
    22381 gggttttact atgatttcca actatatgtc cctcagaaac cattcttttt actaaatctt
    22441 tattttcttt tatatatgga gctgtaacaa aaaaaactgc tttaacctgg ttttgtttta
    22501 gagtgtcgag tatctttgta gtgtatccat tttcataacc ttcatcaaat gtaagatata
    22561 gtgttttttg accatctttt gtaggtccat tgtatagggc atcatagtca gaaaatttaa
    22621 attcaatgtc cgtatttact tctggtgttt tatgcttatt gttaggtata taaaaccaat
    22681 caagagtagt gttatctaaa gaagatacat ctaatttatt attatcatta ttagtagtgg
    22741 cttttgatgg tttatctgtt ccgtctgaaa ctattacatt ggaagtatcc ttaccaccat
    22801 ctattttttc cttatcttct tgtaactgtg tttccttatt ttgattttcg ttttgattgt
    22861 tttgtgaatt tgaacatcca acaataaaaa aaatagaaat agaaagtagt gttaaataat
    22921 gcaatacttt tctaaacatt ttgaagctcc tttctaaatt aataataatt gcatatagta
    22981 ttattataat tggaaattac tattaataat agttatatct ttaaaaataa agtcacaata
    23041 gtatgacaaa gggtgaataa tcaataaatt taaaaaaaaa tgaaaaaaat agttgaccat
    23101 attatgagaa gatgttatta taataaatgt gctcaacaag aagtaacaaa ataacaagca
    23161 caaagaagtt aattgaat
//
LOCUS       DAEPOG010000047        23152 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEPOG010000047 DAEPOG010000000
VERSION     DAEPOG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.047"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            291..1127
                     /locus_tag="KR432_001548"
     CDS             291..1127
                     /locus_tag="KR432_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640092.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1480..3057
                     /locus_tag="KR432_001549"
     CDS             1480..3057
                     /locus_tag="KR432_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640093.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3050..4234
                     /locus_tag="KR432_001550"
     CDS             3050..4234
                     /locus_tag="KR432_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0640094.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4252..4851
                     /locus_tag="KR432_001551"
     CDS             4252..4851
                     /locus_tag="KR432_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0640095.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4875..5345
                     /locus_tag="KR432_001552"
     CDS             4875..5345
                     /locus_tag="KR432_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0640096.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5702..6448
                     /locus_tag="KR432_001553"
     CDS             5702..6448
                     /locus_tag="KR432_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBH0640097.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6510..7184
                     /locus_tag="KR432_001554"
     CDS             6510..7184
                     /locus_tag="KR432_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0640098.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            7552..8898
                     /locus_tag="KR432_001555"
     CDS             7552..8898
                     /locus_tag="KR432_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0640099.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8925..10325
                     /locus_tag="KR432_001556"
     CDS             8925..10325
                     /locus_tag="KR432_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0640100.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            10535..11971
                     /locus_tag="KR432_001557"
     CDS             10535..11971
                     /locus_tag="KR432_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0640101.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            12315..13259
                     /locus_tag="KR432_001558"
     CDS             12315..13259
                     /locus_tag="KR432_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBH0640102.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13428..14360
                     /locus_tag="KR432_001559"
     CDS             13428..14360
                     /locus_tag="KR432_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBH0640103.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14465..14932
                     /locus_tag="KR432_001560"
     CDS             14465..14932
                     /locus_tag="KR432_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBH0640104.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15176..16030)
                     /locus_tag="KR432_001561"
     CDS             complement(15176..16030)
                     /locus_tag="KR432_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBH0640105.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16196..18160
                     /locus_tag="KR432_001562"
     CDS             16196..18160
                     /locus_tag="KR432_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0640106.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            18160..18621
                     /locus_tag="KR432_001563"
     CDS             18160..18621
                     /locus_tag="KR432_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0640107.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18624..18944
                     /locus_tag="KR432_001564"
     CDS             18624..18944
                     /locus_tag="KR432_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0640108.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18960..20060
                     /locus_tag="KR432_001565"
     CDS             18960..20060
                     /locus_tag="KR432_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0640109.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20180..22780
                     /locus_tag="KR432_001566"
     CDS             20180..22780
                     /locus_tag="KR432_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH0640110.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9122 a         2370 c         4070 g         7590 t
ORIGIN      
        1 agttaaaata cttgagaata ctggaaaaaa ctgaaaataa tttttgtaat ataaaatgtc
       61 gattgatttt aaataattaa atatacataa gaaataatta aatatatttc aatgaaaata
      121 tgataaaata tgacaaatta attgatgaca ataaaaaata ttttgttatt gtagtattat
      181 agggtaaatt gcaaaatata tacgaaagta aaattttaaa tattgacatt aaattaaata
      241 tactatacta tgtaagtgtg atgagagaat ttttttagag gggatttata gtgaatataa
      301 aaaagatatt taataacaat gtagtagtaa gctccctaga agatggcaca gaaataattg
      361 tcactggtgc tggagtagga tttaagaaaa aagtaggcga tttgatagat gaaaatttga
      421 tttctaagaa atattttgtt caagacgatc aaagggacaa atacaatcaa atacttaata
      481 agacctctat tgaatacttt aaaatttcag aagaaattat agaaaaggct aatgaagttt
      541 taaatactca agtgaatgat tcaataatat tagccttaac tagtcacata gaatttgctg
      601 tccaaagaga aaaacaaggt ataaaattac caaacttaat attgaatgag acaaaacaac
      661 tttataggga agaatttaaa tttggattat gggcaattga tgaaattgaa aaaaggatag
      721 gaataaaatt gcctgaagat gaagcaggat atattgctat tcacataatc aatggtttgg
      781 aaaattctca aaaagatgaa ggtattaata tactagagtt ttcaaaacaa gttattcaga
      841 taattgaaga agtacatgga tttaaattgg atgtaaattc attaaattat actagactta
      901 taactcactt aaagtttttt gttcaaagga ttctccgaaa agaaacttac aatgatagtg
      961 atattgaaga tatgtataag ttagtgaata aaaattacaa taaacctaaa gtgtgtactg
     1021 aagaaattgc agcattggta ttagacagat ttgaatataa gattagtaaa gaagaagaat
     1081 tatatttaat gattcacata aataaaataa caaacagtgg ttgttaggat tgtaactgat
     1141 aaagcaggca aaacctaagt caatgagctt aactattaga ttaagttttt tgatttaggt
     1201 ttttttatta gatacaatca aaatacagac agtgactttt aggattgtaa ctggttaagc
     1261 aggcaaaacc taaatcattg aatttaacta ttaggttaag ttttttgatt taggtttttt
     1321 tattagatac aatcaaatac agacagtgac ttttaggatt gtaactggtt gagcaggcaa
     1381 aacctaaatc attaaactta attattaaat taagtttttt gatttaggtt tttctataaa
     1441 tttgattatg tttttagata aaaataggag gggaaaatta tgaaaaaaaa taagttaggt
     1501 ttatggaact tcttccaaat gttagggaaa acgtttatgt tcccaatagc actacttagt
     1561 gtttctggga tgatgctagg acttggggca ggttttacag accctaagat gattgaaatg
     1621 gttccatttc tagggaatga atatgttaac ttagttttaa acttcatgtt aacaatagga
     1681 ttatttgcat ttaacaactt gggagcatta tttgcaatgg caattccact aggattatta
     1741 aaaaaagaaa aagagtttgg tgctttttca ggattagtag gatttatagc aatgcatata
     1801 ggtacaaatt tttatttaac aagagctgat ttgttagtac cagcagaaca gatgtcaact
     1861 aatggtcaag ctatgattat gggtatacaa acatataata caagtgtttt aggtggtatt
     1921 gtagcaggat taattgttta tgcaatatat gataaagtta gtaatttaaa gataccagaa
     1981 tcattaggat tctacagtgg accaagatta gttccaattg ttagtttaat agttatgagt
     2041 atagttggat tattaatacc tattgtttgg ccaccatttt tcaatgcatt ccaaagttta
     2101 ggaagatgga tttcttcagc aggaccatta ggatattttt catatgcagt ggcagaaaga
     2161 gttacaattc catttggact aaatcatctt gtaacatctg tattccgttt tacaccaata
     2221 ggtggaactg ctgttattga tggagaaacg tactttggta cattgaacat gtttatggca
     2281 tatgtagaaa ataatcagat tatacctctt gatttagctg gtaaaatgga acaaggtaaa
     2341 ttgatgattc aatatggatt agctggtgct gcattggcta tctaccgtac agcaaaacca
     2401 gaaaatagaa aggcacttaa aggactttta atatcaggag tgttgacagt tattataggt
     2461 ggtataagtg aaccaataga gtttctattc ttattcatat caccagcatt atttgctttc
     2521 catacattta tgaatggtct agcaaacatg gtacttccat acttaggcgt gttgatggga
     2581 ttcacaggag atttaattgc atttataagt tttggtgtgt taagaggaac agctacagga
     2641 tggccaatag cagtattagt agcagtttta tattttgcaa tatactattt tgtatttaag
     2701 tgggcaatta ttaagtttaa tataaagacc ccaggacgtg aggataaaga aataaaagta
     2761 tctgaaggaa atggttcaga tttcaaaaat ttgtctacat ataaaggaca acaaatgata
     2821 tctgctttag gtggtcaatc taatatatta tcactagata attgtgtaac tagattgcgt
     2881 ttaaaactac aagatgtatc attaataaat gaggatgcaa ttaaagaggc tggtggtata
     2941 gcagttgtta aattggatga gcatacaata caagtaatta taggaactca agtgtattct
     3001 ttaagaaagc agatggataa agctatggaa ggtcatttgg aggcatgtaa tgattaattt
     3061 tgataaaaaa gtagaccgat tgggtacata ttgtacccag tgggactatg tagaagatag
     3121 atttggtaag aaggatttgc ttccttttac aatttcagat atggatttag agtttccatt
     3181 tgaaataaca gaggtgttgc aggaaaggtt aaatcataga gttttgggat acagtagatg
     3241 gaaacatgat gattttagaa attcagttac gaattggtat attaaaagat ttaattgtag
     3301 cgttgatagt aactggatat attatagtcc aagtgtaatg tattcaatta gcaaacttat
     3361 tgagatgtta agtaatgaag gagatggaat ccttataaat actcctgcat acaatgcttt
     3421 ttatgatgtg ataacaaata acaatcgtaa tattataaag tcacatcttg taaataggaa
     3481 tggattctat tcaattgatt ttaatgattt tgagaaaaag tgtaaaaaat ctaagatatt
     3541 tattttatgt aatcctcaca atccaactgg tagagtatgg agtgagttag aacttgttaa
     3601 gatgatagga atttgtaaga ataatgatgt caagataata tcagatgata tacacatgga
     3661 tattgtatat gacaatagaa tgacacctat cttaaatgtt gcaaaggact atttagattc
     3721 tgtattcatt tgtacatcag cgtctaaatc tttcaatata ccagcattga ctgggtcata
     3781 tgtgattata ccaaatgaag gtattagaaa taaatttgaa aatatcacta gataccgtga
     3841 ttttgtaaat tcgccagcaa ttctagcaat attagctaca atggtctgtt atgatgactg
     3901 cgaatattgg ttagatgaac tattaaaata ccttaaagag aatcttaaat atacgattga
     3961 ttatataaaa gataatttag aaccattgaa attggtcatg ccagaaggct gttactttgc
     4021 ttggatagat ttttccgaac ttaaaataag tggtgagaaa ttccaaaaat tattgatgga
     4081 tattggagaa gttgctatta tgtcggggtc tgtatatggt gaagaaggtg agttttattt
     4141 aaggttaaat gtggcatgtt caagagaaaa acttgaagat ggtttaaata ggattaaaaa
     4201 aactatggat tatattgcaa aaagaacttt atagtaggaa gggagatagt tatgggatat
     4261 ttaaaaacaa tggttgatgt tatgactact gaaatgaatg ccataagatg tttaatagat
     4321 gaagcaggaa tagaatatga aagtatagtt agtgaaattg caaattgtga aggcaaagta
     4381 atttttatgg gtgtaggaaa atctgcacat ataggtaaga aattggcagc tacatttgct
     4441 agtactggta cacctagctt ttttgtacat gcaacagagg cagtgcatgg agatttagga
     4501 atgattgaaa gtaaggatat tacaatttta atatcaaata gtggaaattc tatggaagtg
     4561 gtaaactgta taaaatatat taaagcaata ggttctaaga caattgcatt tacttcaaac
     4621 agaaattcag tactggcaaa agagtgtgat tatgctttaa tatatcctgc aaaggatgaa
     4681 gcagaccatt taaatctagc accaacaaca tcatctacaa ttactttggt tttaggagat
     4741 tctattgcat gtgcattatc taagagtagt aattttggtt cttcagattt ttataagtac
     4801 catccaggag gaagtttagg tgagaagtta aaaacagcta acaatggata aaagcaaaaa
     4861 ggagagtagt ttttatgttc aaaatgttta aaaaaaaatc aataaaatca cccatatcag
     4921 gaaaagtaat agagttatct aaaatacaag atggtgtatt ttctcaaaaa attatgggag
     4981 agggtgtagc tatagattct acaggagaca ttgtatatgc tccaatcaat ggaaagatag
     5041 tagtagtagc agagactaaa catgcatttg taatagaatt agaaaatggg atggaacttc
     5101 ttattcatgt tggtttagat actgtaaatt taaaaggcga aggttttgag gcattggtga
     5161 gtatgggaga acaagtgaag gaaggaacac caatgttgaa aattaaccgt cctttgattg
     5221 aattaaaggg tatatcactt attactccag ttacaataac taatcattca gagtacaata
     5281 tgaatacatg taatgtaggc aatagcgttg aaggtgggaa agatactgta attgagttta
     5341 aataaagaat taaaatacaa ggttaatatt tataaatacc tataaactta tgttttataa
     5401 gattgattgt atggatttat tgtataaaaa agaactcttt aattataaat taaggagttt
     5461 ttttattttg taaatattgt ttagatttat atagaaaaac acttagtaat aaatagaata
     5521 aataagtatt tatatagcta aaaatctaaa tagttttgtt taaatatatt tttatatttt
     5581 gtaatttcaa attaataaat aattagaatt gataaacaaa aaataaaaat gttgattata
     5641 ttaataaact ttaatataat atatttatga aaaatgtaaa ttgtgaaatg gaggtagtaa
     5701 catgaaaatt gaaaatcttt ctattgatga tattaaacga ggctatatat ttaatgatga
     5761 tttaaattgt tatagttgca ttaaatgtac aaaaacatat gaggtaggag aaatatataa
     5821 aataggtaat agattctttg aagcttcaaa agctattgaa cttcatatgg aattagaaca
     5881 tagtgattat ttagagcaat tgcttaatag tgattcaaag tacaacacat tgactgaaaa
     5941 tcaaaaacaa ttatttcaat tatttattag tgatttacca gataaggaga tagcaaaaca
     6001 ggttggagta tccacatcta ctatacgcca tcaaaaattt attttcagag aaaaagcaaa
     6061 acaggctaag ctctatttag cactatatga atctgtattt gaagataaat ctaataaaag
     6121 tagtactata ataccaatcc atgaacatgc tcgaatggta gatgaaagat atgtagtaac
     6181 tgaagaagag agaaaacaca ttttggatac atcatttgaa agtatgaatc ctttaaaatt
     6241 aaaagtattt tcttcaaaag aaaagaaaaa ggtcgtaatt ttatctaaga ttttggaaca
     6301 atttgaacgt gggaagaaat attcagaaaa ggaagttaat cagattttag aatctatata
     6361 tgatgatttt gcaacaatta gaagatattt aattgagtat ggctttatga gtagaaataa
     6421 agaatgtaca aagtattggt taacataatt aaaataataa attttactaa tgtgtaatgt
     6481 tatttttatc taggggggag gagttaaata tgaatgatga attaaaatct attttataca
     6541 ataaaggtgt agacattgtt cgctttgttg atatatctga atttccaata aatcaaacac
     6601 aaggtttttc taaagcaatt ttattttgta tagggttatc aaaaaaattt attacagata
     6661 tatacaataa cttacctaca gatagtgatg agtttttgga aaaagaggag aaagtagaag
     6721 aattagctga ctggatttca aaatatattc aaaataaagg atatagagcg tattctcaat
     6781 cggagaaaaa caatctagaa catggatatt ttgaaaaagc ctatattaat ccagaaatgc
     6841 aatcagggat tagtccttta ccacataaga caatagcaaa tattagtggc attggattta
     6901 tgggaaaaaa taatttgttt gtaacagagg aatacggatg tgcttttagc atgtgtacag
     6961 tacttacaga tgctccaatt tctgttgaaa gatatccttt gattgattca aaatgtttaa
     7021 attgtaatgt ttgtgtagag aactgtcctg caaaagctat acatggaaat gaatggactt
     7081 tacctggaaa aagagaatct attattgatg tttcaaagtg tttttgtgca ttgaggtgta
     7141 tgatgagttg cccatggtca ttaagatatg caaatcaaaa ataactttac tagagaaggt
     7201 attaaattga atattataaa atttttaaaa tgtaagatta tataagattt ctcaaaataa
     7261 aaatttgtat agaacactca gaaatgagtg tttttctatt gggattaaat tatattgata
     7321 caaagcttaa ctattttaat cattatataa aatttgtatt aaaaaattaa gaataacttg
     7381 ctaatagtaa aaatataatg agattataat tgatttataa tataactgta atgatgttgt
     7441 aaaatgaaaa ttgtcatctc aaaattccca aaaagagtaa aagtcacttc ggttatagtc
     7501 aaaaaatttt ttctgtgaaa agaatttaga tttaaaaggg aggaattaat catggaaatt
     7561 gaaactaaaa ctccagattg gattaatgta ctagaattaa acagtaaaat agatattact
     7621 tggaaatatg atgtaagtaa tatgatacaa gatattgtct ctgataagtc actagaaggt
     7681 tccattatat attttccaaa aggtaattac ttatttgaga aaggtattaa aattagtcaa
     7741 caaataacac ttcaaggaga ttcttattat ggaggagata atcaagtatc aaatttagat
     7801 aaaaaacaat caatcgtagg aacaaccaat tttataaccc gaggagtttc taatataagt
     7861 attattacat tggaaggaac aagtcaatgt atcaaaaata ttaattttta ttatgatagt
     7921 catgatatag agaaaatacc acctaaaaat gtatctgcta ttacagaata tggagaaaca
     7981 caaggactat ttcactttga acacctgttt atttctggat tttcaggaat agggattgaa
     8041 attccatact attcaactgg caatgatatc attgttagtt catgtggtct tggtatgagg
     8101 cttggagaga agtcaatgct ttctagtagc aagatttatg aatgtaaaaa tggaatggag
     8161 attactactg gtgttagctt aaataatgtg agaatagaag aaataagaga aataggtatt
     8221 aataataaag gatttggttt taacctgatt atgaatctta tggtcgacca atgtggatat
     8281 tgtggattta tgtttgaaaa aatgtgttat agccagataa ctgccagaat tactaaatgc
     8341 ggtcagtatt ataaaagtgt tgattataat acttatgaga atatggaaga tagaaaagaa
     8401 gaagcttatt ccatttttta tggagatatt ttagaaaatt gcaatattgt attgatgaat
     8461 agtaatattg ataccttaga aaatgattca cttgatgaac ataagatata tgtaatcaag
     8521 gcaaatgaaa caaacaaagt aatgttaaca tgttatgctg aagcagatga gcttatacag
     8581 tgcgcaaagg gcaacttgtt gctagaaaat ggtgaaaata cttataaatt ttataatggt
     8641 gagatttgtt ctgtaggagg tgttcaaatt tctgatatag atgaaaaaga tttaatcaaa
     8701 ataatcgaga acactctgta tatagatgat aaaggtaagt acttaatggt tcctagatta
     8761 aaaaagaata ctgtaatata ttcagtacta gattctatag aagaaataaa taaattacat
     8821 ggtggaatat gggaacagat tgatgtaaaa gtattagctg gggaatctat gtactattat
     8881 aaaaagataa aagattaaat tatcaaacta ggaggtagtg attaatggaa gagtctataa
     8941 taattaaaga tataagtggc tggattaatg tacttgatta tgggatttgt aatgatgaaa
     9001 caaaagatgt aagttccaag ttgcaagaga ttatagatgc tgcaccagaa gattctatta
     9061 tatattttcc aaaaggaaaa tacttatttg aaagtggtgt taagattaag aaacgattaa
     9121 ccttatgtgg agattcttat attttggcga atgttcctaa aaacactgct ggagtaacac
     9181 aatttacttt taaaggaaag aaaaatccta aaagtgaaaa tatagcaatt ataacagcaa
     9241 aaagtgtaag acactgtatt aagagcattt gcttttattc agatagttgt gatagagata
     9301 tgacatctaa tcatgaacct ccaacagatg gaaaacctag atttcatcat aatgtaatta
     9361 taaattatag aaatatatcg gctattgtat gtgaagataa acagggagaa ccaggtcatt
     9421 atgaaaactt gttttttagt ggtttttctg gaattgcact tgatatgcct aataattcta
     9481 cagcaaatga tattacagtt tttacatccg gacttggaat tagtacaggg gaaaattcaa
     9541 cgattgctaa cagtaaggtt tggggctgtg ttgatggaat gataatttct actggaactt
     9601 ttataaatgg tatgagggta gaagaaataa aaaaagttgg aattaaaaat attggtagag
     9661 gtttaaattt tattacaaat atcacaattg accaatgtgg atattgtgga gtttggtttg
     9721 attctatatc caatgttcag atatcaggaa atatcacaag gtgcggacag tactattatg
     9781 atgtaagcta tgataattat ttaatgttgg ataatagagt taaagaagca tattctttat
     9841 tctatggtaa tttattggag tcaagtaata tagttttgtc aaataccaat aaagataatt
     9901 gggaagattt caaagaggat aagcataagg tgtatcttgt agaagcactt gaaaccaaga
     9961 atgtactatt aacatgcaat atagatgcta ctgactggat tgttaagtca gaaaaaggaa
    10021 atttaacgta caataatatg cgacaaactg ttatatttta cgatggaaaa ctaagtagta
    10081 tcaatggaat tggtatatct gatgaagatt atggggataa agtcaagata aaaaaaggaa
    10141 atatatatat aaatagtaag gataaggtta ttaagatgca taaaccagag gtaggtgaca
    10201 ttatgtcaac aattattaaa gatgaaaata tactttccgc tcattatggg ggaacatggg
    10261 ggaaaattgg tgaaaaaata gagtttggaa taactgtaca ctattataaa ttgatagaga
    10321 aataagtaaa tacgtagtat ttgaatagat gtattttatt ggagagctag gactatagtt
    10381 ttaatgtaaa tggtttggta atagcatgga ttgaataata taaaaataat ataaaaaatt
    10441 aatttgatta tgtaaaattc tatgaagtag taaacctgtg actttagtca tggtaggttc
    10501 aaattaagta aatgggaaag gaatatataa tattatggaa ggaagaactg ggattaaaga
    10561 tggaagatgg attaatgtag tagaaagagg tattaaaaat gatggttcgt acaacatagg
    10621 tcctgattta caggatatta ttgatgaaat agaaacagat ttctgtgtct tatattttcc
    10681 aaaaggaaaa tacttattta caagtggtgt taaaattaat aaagaaattg tattacaagg
    10741 tgattctaat tcacctacag agactacaca atttataact cgtggagtag aaaatatgag
    10801 tattattaca ttgacaggga agaaacaatg tattaaaaat ataaatttct attctgatag
    10861 ttgtgagata caggtaaatg aagaaccacc gacaaaaggt aatcctaaat atcattatga
    10921 gatgattatt aataagaatg aaaaaaatgt tgagatgaat aatgtatcag caattctgta
    10981 tgataatagt aagaatatga ttaaaggtct aggtcattat gaaaatattt atataagtgg
    11041 tttttcgggt acaggaatta ggataccata ttactcaatt gtaaatgata ttacagtttc
    11101 atcttgtggg ttgggtattg atacaggaat agacacaatt atttctaata gtagaatatc
    11161 taagtgtcaa aatggaatga ggattagaac tggaactagc ataaataatg taagaattga
    11221 aaaaatacag aaagttggtc ttataagtag tcaagttaag gattatgagg gatatggaag
    11281 ttataaaatt aataatatta cgattgacca atgtgggtat tgtggttttt tatttgactc
    11341 agtgaaaggt agttattttt ctggtattat aagaagatgt gggcagtatt attataatac
    11401 agattatgac acatatttaa gtattaaaga tagagtagaa gaagcatatt ctttatttta
    11461 tggaaattat tttacagaat gtaatattga tttagttagt gataataagg ataactggga
    11521 tgatggctta gaatataaac ataaagttta tgtatttaaa acccttcaaa taccaaaatt
    11581 aacattgaga tgcaatatag atgcctatga ttatatcgtt aaatcggtac atggaggagg
    11641 taatttaatg ttaaaaaata actttaacaa ttatatattt attgattcat ctagtccaga
    11701 agttattaat ggaattaatt ttaattatca aaagaatgga aacctgttaa ggttaaataa
    11761 tggtaaatta agttataaaa atggaaagga agaaacagct atattcagac ctaattatgg
    11821 tcttattgta gcatcaacag attcagaatg tgaacaaata gagactagct atggaggaaa
    11881 atgggagaag cttggttctg aagtaattgg aaaagaaacg gtatattatt ataaacatca
    11941 tgaaccagat aaaacaaacc aaataaaata aaatttgtat tatataatga tataaagttt
    12001 taatatatag tgtttacctt taatgtaata ttggtatatt tgtatagtat taaatttgtt
    12061 aaaaagtaaa attgtagggt gcttgaaaat agcacctttt ttagtttcat tataaaatta
    12121 gttttgaaag tttaggaata atgtatattt atttttaaag aattataacc ttattaagga
    12181 tattttaaat gctagaaatg aatcagtaaa tcatactgga attatcttaa atgttttgat
    12241 taaattaagt atttagagct ataattatat taaaaatata ttaggacata attatattac
    12301 tatggaggtt aaatatgata gtaagtgtga gtagaagaac tgatatacct gctttctata
    12361 gtaagtggtt ttttaacaga cttaaagaag gttttgttta tgtaattaat ccaatgaatc
    12421 caaaacaggt tagtaagata gagttaaatc cacatactgt tgattgtttt gtattttgga
    12481 caaaggatgt aactccaatg ttatatgatt taaataaatt aaaagactat aaatattatt
    12541 ttcactatac tataacatca tatggaaaag aagtagagac atgtatttta gataaaagaa
    12601 aggtaataga ttcatttaag gaattgtcta gaaaaatagg caaagaaaga gtaattttaa
    12661 gatacgaccc gattttctta agtgaaaagt acacagtaga ttatcatatc aaagcatttg
    12721 cgagtttatg taatcagctt gatggattta cagaaaaatg tataataagt tttatcgatt
    12781 tatataagaa aacaaaattt aatactaaag cattacatat aaagccaata gaaattacag
    12841 aaatagaaac attatcaaaa gaaatttcta agattgctca taagcatggg ataatacttg
    12901 aaatgtgttc tgaagaatat aatttaagta aatttgggat tggaaaaagt aagtgtattg
    12961 atgatagatt aatatcaaaa attataggat gtgaagtaga ggtaaaaaaa gatagtacac
    13021 aaagagatat ttgtggatgt gttaaaagtg tcgatatagg acaatacaat acttgcagac
    13081 attattgtct gtattgttat gctaacttta attatgcaaa agtagaagaa aactgtaggc
    13141 tttataaaga agataataaa ttactcatag atactttaag agatgatgca aaaataagca
    13201 ttagagatat gaagactatt aaagtagata agaataaaca aattagtata tttgattaaa
    13261 ataaatatac taattacctt aatatatcaa gaatatagat acttttgata acaaaattaa
    13321 acttaataga tattttctat ataagaaaga tggtcataat atgtcaattt gaccatcttt
    13381 ttgatataat taaaacacaa ataatttagt aacaaaggag gcagaacttg aatttattaa
    13441 aaaaatatcc tatacctatt gcaagtttga ttttgtcaat ttttacttta ggtaacttat
    13501 tacaatctta tagtgaatca ttacgtaatg taattggatt tataggattt attttgtatg
    13561 caatttatat agtcaaaata atattactta gaaagggtgt taaagagcaa ttagagaacc
    13621 ctttaatatc aagtacattt ccaactataa caatggcaac aatgatattt gctacatata
    13681 taaaaccatt atctttagaa ttaagtatta tgatttggag cataggaatt gtgggtcatc
    13741 ttgtgttaat agttttattt agtaagaagt tcttagttaa attctcaata aaaacggtat
    13801 ttccatcttg gtatatagta tatgttggga tagttgcagc tagtgttacg gcaccagcat
    13861 ttaatcaact tttgattggc aaaattgctt tttggatagg atttgtatct tatatagttc
    13921 ttataccagt aattttaaag agagtatgga taatcaagga aatgcctgaa ccatctttac
    13981 caagtatagc tatattagct gcaccagggt cactattatt gtctggatat attaattctt
    14041 tttcagataa aaataatgta gtattatact tgttattcat attatcaatt gtattttata
    14101 ttatagtttt gggatactta ccgaaattat taagattgcc tttttatccg agttttgctg
    14161 catttacatt ccctatggca ataagtgcaa tgggtatgaa actaatgaat ggttatttaa
    14221 ctaaatcagg tcattttata agttggattt catatgcttc taagttagaa gagataattg
    14281 ctacagtgat tattttatat gttttagttt tatttattat atatcttagt aaatctgaaa
    14341 ctacatctaa agctaaataa gaattattaa atatgtgtat ttaataggaa tattgatata
    14401 aatatatgaa gataagataa accatagaaa cttaatttag cttaatttta ttggaggaaa
    14461 acaaatgaaa acaaaagaag aatatttaaa agaagttaga aacatatcag aagaatactt
    14521 tagaaaagga gagttttttt gctctgaagc agttcttcag acaataaatg atgctttagg
    14581 ccaaccttta agtccagaga taactaaatt agcatcagga tttccgatag gacttggaaa
    14641 agctcaatgc ctttgtggag cgatatctgg aggagagatg gctttaggta tagtatatgg
    14701 aagagtacat ggagaaacta tgaatccaaa gatgtttgag catgctaaag gtttgcatga
    14761 ttatattaaa aaagagtatg gtgcaacatg ttgtagagct attacaaaga aatgggatgg
    14821 tgataacttt atgtctcccg aaagaaaaga gcactgtatt aaaataacag gtcaagttac
    14881 agagtgggtt gcagataagc ttatagaaga tgaaaaactt aatatcaaat agtgtaatat
    14941 tagttggtga atttataaag ttaaatttac ttatagtata agatacttga tattctcgta
    15001 ttgatagaaa aatttatagc atatgtattt aatgaaatat acttatattt agagtgtggt
    15061 ttaatttcat aaatacatat gctattattt taagtatata agcaaattta tttaaataaa
    15121 tagaaacagt taatttatat gtacaaaact atatatgatt agtgtaaata aatacctatt
    15181 ttataatact caacataaag acaccagaca tgattagtat tataccaata aaaatagcaa
    15241 gccattcttt tttgttcttc ttttctttaa aaaatataat ccctcctaaa gtggctacta
    15301 tcacacaact ctgagagata gaaaaactta ttgtaactcc caatgtatta gtcgttatga
    15361 acatacctaa atttgctatt gaccacaata taccagtaat taaattgaat aaaacatttt
    15421 ccctatatac aatctcttta gaaaaatact tgacaataat agatgaagtt atcaacatac
    15481 caacagattg aggtaatata attctatatc cttcaattaa aaatatttga ttagttacaa
    15541 catataatga taaaaataga gatgataata atacaactcc aacacatttt aacatatttt
    15601 tatctgtaga ttttttagtt tgataattag tgagaagtat accaagtatt atacataata
    15661 ttgataaaga accaatcagt aacattttag aactggtcca ttcattaaac aagattacag
    15721 ctaataatgt cgtaaaagtt aattgtgaac catttgaaat tggcattact ttagatacac
    15781 tagcaatttg aattgcttta aactgcatta attgtcccaa actccagaat atgccagaaa
    15841 tcatacttat acaaaatgaa aaaaatgtaa tttcaggatg tataaccaga gttattatag
    15901 tggaaaatat aaacgcactt atggatgtac ctaaaagctg ttcatgagga gaacttttct
    15961 ttagatttgc aactatgggc atcaatcccc atcccattac aggaagtaaa gcaataatta
    16021 ttgtcagcat taaactattt cctttcatat taaatttaga cagcttttaa tttatcatct
    16081 aaaattaaag tgttcaatac ataaaaatta ccgtatttaa tcaggaaata ttagtagttt
    16141 tttagaaaaa cattttccat taaaataaag ataaagggaa ggagtaaaat tgaaaatgtt
    16201 aactgatatg attcaaaatc gtgaaattaa aataataaag tttttattaa aagaaggtca
    16261 tacaactgta aaagcaatag cagatgattt agatgtttca gaaaaaacag tttcaaaatc
    16321 tcttaaagaa gtagaagcag tgcttaaaaa gataggtctt tctttaataa gaaaacctaa
    16381 gataggtgtt tatattgatg gagatttaaa aaaaatactt ccttacctag ataataaagg
    16441 aagtcaaatt cctacaacca gagaggagag agttatctat atatattcag tacttttaaa
    16501 gagtaatgat tatattacta tacaacaact ttctgatgaa ctatatataa gtcgtggaac
    16561 tattgaaagg gatttatcag aagttgaaaa actgctaaaa aacgaaggga tttccatata
    16621 taagaaacct agtaagggaa taaagttaaa tataagtgaa agagataaaa gacaacttac
    16681 tgctaagttt atacataaat tttggagtag aaactggtat attaagcaag agcaagaaag
    16741 cttttatcaa gcatttgaaa agatacaggc agatgtaaat ggtatttttt cggaagatga
    16801 tttatcaata attattgata ttcttaggaa tttcagcaga gaaagaaaat ttgagttctc
    16861 tgattatgaa tttcagtcat tagttattca tttggcaata gctgtagaga gaattagaaa
    16921 aggcgaatat atagaagaaa gtttgtatat tggtaatata gaaaataatc aaatgtcaaa
    16981 taccaattat ttagcaaaac aattagaaga aaaattttca atattattac caaaatctga
    17041 aattggatat ataaatttac atttagtagc ggctgaccaa catagagata ctaatgaact
    17101 aaaaaatact tcaactaatc cttgtgaaac aagtacaaat aatatatcaa atcttcggga
    17161 aattataaaa aatacattgt atgagacagc ttatgacaag gatttaattg atggattgac
    17221 ttcacatgta cattcatcaa taaatagact gaaatatgga cttagtatta aaaacccata
    17281 tgtaaataca atcaaacaaa acttttcttt atcatttgag caagctttag atttaaagaa
    17341 agtaattgaa tttatatatg atattagaat aaatgatgat gaatgtgctt atatagcatt
    17401 acattttgaa gcttttagag aaagaatgaa agaacttcca caaaatatta gagtaattct
    17461 agtatgcagt acaggattag gttcatctag gttattgtct gcaagaataa aaaaatactt
    17521 tccaatgata aagattgaaa aagtactttc cgtacaagaa ttagtatcca gtaaaattga
    17581 tgcagattta gtaataagta ctatttattt agagttagaa acaattccaa ccatagtagt
    17641 tagccctatt ttaaatgaaa gtgatataag aaccttggaa agaaatatac aaaatgtttc
    17701 atgttgtact tctaagaagt ataaatcttt cagaaaccta atctttgaag aaaatatatt
    17761 gttaaatatt gaagcttcaa actatgaaga tgcaattaag tatataacag ataacctaat
    17821 aaaaaaaggt tttgtaaaag aaggtatagc taaaagtgca attgagagag aaaatttatc
    17881 ttacacgtct tttcagtcaa tagctacacc tcatgctaat cctagttata ttaaaaaatc
    17941 taatatatct gttgcaacgt taaaaaatcc tattacatgg ggaaatgaaa aggtggaagt
    18001 tattttcttt atttcattag aaaatgacaa aaacctagag cttgatgaaa tatatgaagt
    18061 tttttatgac tttattgaca ataaaaaaaa tataaatgac atattaagtg caacttccac
    18121 aaaggaagtg tacgatttgc tattggagga gagtatatag tgaaaatatc agatgtattg
    18181 aaaaaagagc aaattgtttt taatcttgat acaaatacaa aggaagaaac aataaaaaaa
    18241 ttaagtagta tatttgttga aaatgggatt gtggacaatg aagaagaata tgttaagtct
    18301 gttttggaaa gagaagaaca ttcaactaca ggtattggta atggtattgc tattcctcat
    18361 ggaaaaagtg atgctgtcaa aaaatcttct ataatatttg caaagactaa gcataagatt
    18421 gagtgggatg ctttagatga aaaaccagta gatagtattt tcttattggc tataagcaat
    18481 attgataagg atagcaatca tcttgtacta ttatctaaat tagcaactaa acttatggat
    18541 gatgacaatg tagatgcact aaaattagcc aattcagaac aagaaataat caatatattt
    18601 agtgaagaag gagagatgta gaaatgaaaa gaaaaattat agcagttaca gcatgtgcaa
    18661 caggagttgc acatacatat atggcagctc aagcgttaaa gaaagcttca aaaaagatgg
    18721 gtcaccttat aaaagttgaa acacaaggag ctacaggaat cgataatgag ttaactacaa
    18781 aggatgcaca aattgcagaa gtagtaatat ttgcagtaga tactaaagtc agaaatgctg
    18841 aacgttttga gggaaaagaa atactagaag taccagttaa tgcacctata aaggatgcag
    18901 agaaagtaat aaatgatgca ttaaagttaa ttgacactaa ataaatggag ggaaatgtaa
    18961 tgttgaaaaa gatagtctct gagttaaaaa agcatgtctt aactggaatc tcatatatga
    19021 ttccattagt aattgcaggt gctatgataa tggcaatatc tcgtgtaggt ggttctttct
    19081 ataatatacc agatatatgg gatgctaaat acgcagaaag tgctagtagt attgttagat
    19141 tattacatga tctagatgga tttggaggta ctgcattagg attaatgttt cctgtaattg
    19201 ctgcatttat aggattttct atagctgata aattagctat tgtaccaggt cttgttggag
    19261 gtatgattgc taaagatata ggtgcaggat ttttaggagc tttggcagtt ggtcttatag
    19321 caggatatac atgtttgttt attaaaaatc atgttaaatt accaaaatca gcagcttcaa
    19381 tagtaccaat atttatagta ccagtatttg gaacattaat cactgttgta ttaatcaatt
    19441 atgtgatagg tatacctttt gccacattaa atactggatt agaaaattgg ttaaatggat
    19501 tatctggtac aaatcagata ttaatggcag ctattatagg ggctatgatg gcagttgact
    19561 taggtggacc agttaataaa gcagctttaa caacatcact tgcattatta acaagtggaa
    19621 tttatgcacc taatactgct gcaatggtag gaatagttat accaccacta ggattgggac
    19681 ttgctactat actagcaaaa caaaaatata ataaacagtt acgtgaagct gggaaatcat
    19741 ctcttataat gggtttaatt ggagtaagtg aaggagcaat tccttttgcg gtagaatctc
    19801 cattaaaagt aataccttca tgtgtaattg gaacagcaat tgcttcagct atggcagttg
    19861 gtttaggttc agtaaatgca actccaatta gtggatttta tggatggttt acagtagaga
    19921 attggccaat gtatgtgtta tcaatagcag ttggaacagt aattgtagct ggattagtta
    19981 tagtattgcg tggaaatcca gtgattgagg atgatgaatt tgaagatgat gattttgatg
    20041 aagcagagtg ggaatcttaa aatttaataa ctatttaaga cgtgttataa gctatattca
    20101 atatgaatct tatactaaat aaagtgttaa atattcagtt tagacttaaa tataaaagat
    20161 aaagaacaga ggagaataaa tgataaaagc acatattgtt aatcatacac actgggatag
    20221 agaatggtat tttacttctg gagatgcatt agtacttagt gaacaactat ttacagatgt
    20281 aattgatgaa ttagaacgta atccagatgt aagttttgta cttgatggac aactttctat
    20341 tttggatgat tatgtacaat tacacaaaga aaaaatagaa atcattaaaa agttaataaa
    20401 agaagataga ctacatattg gaccatggtt tactcaaact gatgcattct ttgctagagg
    20461 agagtctatt ttaagaaact taatgattgg aatttttgaa agtaaaaagt atggtaagta
    20521 tatgaaaatt gggtatttgc cagatacatt tggatttaat gcacagatgc ctatactatt
    20581 aaagcatgta ggtcttgata atatattctt ttggagaggt atacatcttg gtaaacatgt
    20641 tcaatcacca tattttaagt ggaaaagctt aaatggagaa agttatatat atgcagtaaa
    20701 tatgccacat ggatatggaa ctggtatgct tttagagcct tcattaaagt atgttaatgg
    20761 acgcttagac ccagctattg attttattaa atcatatact gatgtagatg aggtgttaat
    20821 accttctggg aatgaccagt taaatataat aggtgatttc aaaaataaaa ttaaagcgat
    20881 taatgatatc ggaaagtatt catatataac gagttcatat caagagtttt taaaatatat
    20941 aaagtccata gaatcactag aaagctatag aggtgaattt agagaacctg tattagcaag
    21001 aatacataaa tcaattggtt ctagtagaat ggatataaaa ttagcatgtg ataaactaga
    21061 aaacaagctg attaaaagaa tagagccatt attagtaatt gctaaaaaga gtaaaattga
    21121 aataagcaat cagttactta taaatacatg gaaaaaactt ttggaaggac aagcacacga
    21181 tagtctagct ggatgtgtaa ctgatactgt aacagattat attcttcacc gtataagaga
    21241 agcaaatgaa atatgtgaca gtattgaaaa tactattgta aaaaagattt cagaagggct
    21301 taaattatct aaaaatgata ttttagtatt taatacagaa gctaagcgtt ttaatggata
    21361 taaagagata caagttgtaa gtgatagtaa aaatatttat tttaaagata atttagatgc
    21421 tactataatt gaagaaactt atgtaaaacc gagagaaaat gtattagaag aaacacctgc
    21481 tggcaacgta tttattgaag aatcaggata ttatatatta aaagtaagaa taaatgtaca
    21541 gcttcctgca cttggttata aagttgttag ttttgaatca agtgataaag aaatggtatc
    21601 acttgataaa tctaatgaca cttttatatc taatgataat tataagataa tctatgaaga
    21661 tggaagctta aatttacaac ttaaagatgg aacttacata tcaaactttt taatattaaa
    21721 agatagtggt aatgcaggcg atacctatga cttttctcca ttaaaaaatg acgaggatat
    21781 aaaactttca tttgatagag tgtatacaga aaaaacttta ggatatgaaa agttggtggt
    21841 taaaggtact actttactac cacttacatt agaagataga aaaaacaaat atcttaatgg
    21901 gaaattaaat gtaaaagtaa gtattgtctt atccaaagaa aatccattga tggatgtaaa
    21961 actttgtgtt gataacacga tatataatca tagactacgt gttcatataa aaactgatat
    22021 aaaagataat aaaaacatag catcattgcc atttggatat ataacaagag aaaatggagt
    22081 tttagacaac tgggaagata tttacagtga aatgcctata gatttagaac ctttggaaag
    22141 taatattact ctaacaaacc aaaatagaag ctgtactgta tttactagag gaataaaaga
    22201 gtatcaacat attgaagatg aaattgcatt gaccttactt gctactacag atgaactagg
    22261 aaagcctgat ttattatata gaccaggacg tgcttctggt gatacaacta aaaaaggtca
    22321 tataagaatt aaaacagata aagctcaaat gcttaagaaa ttagaatttt catttgcaat
    22381 atatatggat tctaaatcat ttgatgaatt ggaaattgcg aatttaaccc acaattatct
    22441 aagggaatgt gtaaattatc aattacagga ttacaatttc tttttatatc gtatagataa
    22501 taagattcaa aaatcaattg tcaataaaac tgtaagtaga gaattagaaa taatatcttt
    22561 accagaaaaa tatcttatat cagcatgtta tccttcttat tacagtaaag ataaatttat
    22621 aataagagtg gaaaatccaa gtagtaatag aatgatattg gatgagcaaa tatttaaaaa
    22681 tagaaatggg aaaattgtaa atgcaataga agatgtagaa gaagaacagg tatatgaaat
    22741 agcacctttt gacgttattt caatattatt ggatttataa gtgtaaaagt aagtttattg
    22801 tttaaagtct gacagatatt aatctgccag actttttaat ttgtataaat ttgggtaaat
    22861 attaatgaat agttttagta tttaaatatg aatttagtat ttaatataga atttattgaa
    22921 gtagaataat tagtttaaca ggataagcag tttaaataaa gaattattat attagagtga
    22981 ttttatatat aatttaaaat aataaaatgt taataaaatg agttaaagat tgtatattaa
    23041 tagggttgat gtagttacaa aaaagttacc aattgttatg tatttacaaa actgatatat
    23101 taattacata aaatctaaaa tagtttgtaa taggagtata tataataaaa at
//
LOCUS       DAEPOG010000048        22511 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEPOG010000048 DAEPOG010000000
VERSION     DAEPOG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.048"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            65..1363
                     /locus_tag="KR432_001567"
     CDS             65..1363
                     /locus_tag="KR432_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBH0640111.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            1335..3284
                     /locus_tag="KR432_001568"
     CDS             1335..3284
                     /locus_tag="KR432_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0640112.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            3721..3858
                     /locus_tag="KR432_001569"
     CDS             3721..3858
                     /locus_tag="KR432_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640113.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            4086..4460
                     /locus_tag="KR432_001570"
     CDS             4086..4460
                     /locus_tag="KR432_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBH0640114.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            4476..5123
                     /locus_tag="KR432_001571"
     CDS             4476..5123
                     /locus_tag="KR432_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640115.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            5503..6582
                     /locus_tag="KR432_001572"
     CDS             5503..6582
                     /locus_tag="KR432_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0640116.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            7404..7617
                     /locus_tag="KR432_001573"
                     /pseudo
     CDS             7404..7617
                     /locus_tag="KR432_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            7729..9342
                     /locus_tag="KR432_001574"
     CDS             7729..9342
                     /locus_tag="KR432_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0640117.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            9371..9694
                     /locus_tag="KR432_001575"
                     /pseudo
     CDS             9371..9694
                     /locus_tag="KR432_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            9726..10913
                     /locus_tag="KR432_001576"
     CDS             9726..10913
                     /locus_tag="KR432_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBH0640118.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            11105..11509
                     /locus_tag="KR432_001577"
     CDS             11105..11509
                     /locus_tag="KR432_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH0640119.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            12079..12939
                     /locus_tag="KR432_001578"
     CDS             12079..12939
                     /locus_tag="KR432_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640120.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            12929..14800
                     /locus_tag="KR432_001579"
     CDS             12929..14800
                     /locus_tag="KR432_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0640121.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            14820..16274
                     /locus_tag="KR432_001580"
     CDS             14820..16274
                     /locus_tag="KR432_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0640122.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            17298..18506
                     /locus_tag="KR432_001581"
     CDS             17298..18506
                     /locus_tag="KR432_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640123.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            18537..19916
                     /locus_tag="KR432_001582"
     CDS             18537..19916
                     /locus_tag="KR432_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640124.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            20022..21212
                     /locus_tag="KR432_001583"
     CDS             20022..21212
                     /locus_tag="KR432_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640125.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(21340..22404)
                     /locus_tag="KR432_001584"
     CDS             complement(21340..22404)
                     /locus_tag="KR432_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640126.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
BASE COUNT         8295 a         2714 c         4277 g         7225 t
ORIGIN      
        1 agaaaaaatt gtgataatct taactaagaa attctattta ataaatttat cgaggaggag
       61 agtaatgaat ttaacacaaa aaagatggat tatattaatt gcaagttgtt tcatcaattt
      121 atgtattggt tcaatatatg cgtggagtgt atttgcatca cctatggcag aacatttaaa
      181 tagtcttgct ggaacaactt ttacagctgc tagtttagca attgtattta cagttacaaa
      241 ctcagttgga cctattacta tgattacagg gggaatgata aatgataaat ttggtcctaa
      301 aaaagttgta tttgtaggag gattaatttt tggtataggt atgatattgg caggtttctc
      361 aaaaggattg cctatgttga ttttatcata tgggataata acaggtcttg gggttggcat
      421 ggtttatgga tgtacaataa gtaattcaat taaatttttt ccagataaaa gtggattagt
      481 tggtggtata acaacagcag cttatgggtt tagttcagta ataatgcctc cagttgctaa
      541 ttggataatt aataaatatg gagtaacttc tgcatttaaa tcaataggtt tagtatttct
      601 tataattgta tgtgtttctt ctttctttat caaaaaatgt ccagcagatt tcacaccaga
      661 aggatgggta ccaccagaga aaaaaggaaa agtaagagtt gttattaata aaaattggaa
      721 agaaatgctt tcaacaccta tattttatct aatgattata atattaactt gtggagcttt
      781 ctatggatta atgtgtgtgt cattagcatc ttctctagca acaaatatga ttgggatgac
      841 aagttcagat tcagcaatag tggtttcaat tttagccttg tttaatacat taggccgtat
      901 tgttgcagga tatatatcag ataaaattgg tagaataaat actttatcag gtgcattagt
      961 tatttcaatt tttggtttat tatgtttgtt attctcaaga gaaggagatg tagcaacatt
     1021 ctatattgga ataataatta taggtatatg ttttggttca tttatgggaa ttttcccagg
     1081 atttacagta gataaatttg gacctaaaaa taatagtgta aattatggaa tcatgtttat
     1141 tggttttgct tttgcaggat atattggacc tgcagtgatg aaaaatgtat attctgtaga
     1201 tggcaattat caaagggctt tcattatagc tataattata gctttaatag gttttatatt
     1261 aacattttta tataaatata taaataaaat acaaaattta aagatagaaa atttaataaa
     1321 gggagatata aaaaatggaa agaaaatatc caaatctatg taagccaatt aaaattggaa
     1381 atgtaacatt caaaaacaga atgttttcag cacctatggg gggaacagac ataacagcag
     1441 attgtacaat tggctcaaaa tcaacagcct tttacgaatt aagagcaaaa ggtggagcag
     1501 gtgcagttac aattagtgaa tgtatggttc atccagaaac agatggttca catgcatatc
     1561 atctagactt aaaaatagtt gattccttag caagttttac ttatacagca gatgcaattc
     1621 gccgtcatgg agcaatacca agtgtagaac tatctcattc aggacaatat tcaggaactt
     1681 atttagttga caaagataag aagcgaggat tatcacaatg gggagtaagt cctagtgttc
     1741 gtcctgatgg attagagata aaagaactta cagaagaaat aattgctgaa atagtagagt
     1801 catatggaaa tgttgcagca ttggctaaga gagcagggtt tgagatgatt atgattcatg
     1861 gtggacatgg ctggctaatc aatcaattct tatcacctta ctttaataaa agaactgaca
     1921 aatatggtgg ttctttagaa aacagagttc gttttgctaa agaagtttta gatagtgttc
     1981 gtaaagcagt aggtccagga tttccaatag agtttaggat gagtggttct gaattatttg
     2041 agggagggta tgatttagat tatggaacag aaattgctaa attattggaa tcaagagtag
     2101 atttacttca tgtatcagca ggtacatacc aaagagggtt tgcagtaaca catccttcta
     2161 tgttcttaga acatggatgt aacgtatatc ttgcagaaga aattaaaaaa catgtaaata
     2221 ttccagttgc tactatagga gcattaaatg acccagaaat gatggaagag ataattgcta
     2281 gtggaaaagc agatgttatt tacatggctc gtgcattact agctgaccac gaattaccaa
     2341 gaaaagttat gagtaatcaa gatgaaaaaa tagtgaaatg cctaagatgc ttcacttgta
     2401 tggcagaacg tgctaccact tctacaagac gctgcactgt taatccactt attggacgtg
     2461 aattagatgg aactgaagtt gtttcagtat taaaacctaa aaaagtatta atagcaggtg
     2521 gtggaccagg aggattgcaa gcagccatta cagctgtaaa gcgtggacat aaagtaatac
     2581 tttgtgaaaa aactaatgaa ttaggtggaa ttttaaaggg agaacaagca cttccattta
     2641 agtataaaat gtatgaatta ggaaatactt ttggaaaaat agcaaaagat ttaggtgttg
     2701 aagtacgttt aaatacaaca gttacaaaag aatatgttga aaatgaaaat gtcgatgcat
     2761 taattatagc agtagggtct gaacctcttg ttccaccaat agagggtctt gatggagaaa
     2821 atgtagtaat agttaatgat tattacgtag aaaaagaaaa agtcactgac gaagtaatag
     2881 ttttaggtgg aggattagca ggatgtgagg ctgcaattca cttagctcaa gaagagaaga
     2941 ctgttcactt agtagagatg agagcagaac ttgccccaga tgcaaatata aggcatcgtc
     3001 caatattatt agaagaaata gaaagacaag gtatatatgt atatacagaa cataagggtt
     3061 taagtgtaac aacagaaggt gtagtatgta tgaataaatc tgggaataaa ataaatattc
     3121 caggaacctc agtaatatgt gcacttggac aaaaaccaag aagagaagtg gtagacactt
     3181 tacttgattc tgctccatat gttgctcaaa ttggagactg tgttcgtgca tctacaataa
     3241 caaatgcagt ataccaaggg catcatgctg cgctagatat atagttaaaa agacgtagat
     3301 agtattgttt tattataaaa aaggagtgtc tcaaaatgaa ctttttagtt catgagacac
     3361 ttttttacat gaaattaaat atatttttat catttcaaca atgaaaaaag attatcttta
     3421 ttttgagaca gcacttttgt tattatgatt ttaattctta ttgcaagttt tcatttgatt
     3481 gatgagtaaa actttttaag atgtagaagt atttaacaat gtaaatgacc ctaacttaaa
     3541 tactttagcg aaggttgaca gatttgtgtt tgcttttatc tattataact taaggaaaca
     3601 tgttacctga acaataaatt tatataataa aagtatataa atatatatat gaatgttaag
     3661 attagttgat agaatttaaa taataaatcc aatattataa acataggagg tatgaaaaat
     3721 atgattaatg agaatttaaa aagtctaaga aaaatccacc agtatacaca agaagaatta
     3781 gctgaaaaac ttaatgtttc tagacaatca atagcaaaat gggaaagtgg agtttcccaa
     3841 acaaaaggat attactgaaa taacctgtgt ttacctgctt gtagagaaaa gaatattgct
     3901 attaagccag agatactaca taaatgtgtt ggtatctctt tttcgttaat gcaacacagt
     3961 aaattatttc attttcctaa ttattttaga ctgataagta actttttaaa tgaagaaaag
     4021 aaatgacaat acttaaagga gcatggtata cttaaaggag aatttcatta aggaggaaaa
     4081 agagattgtt taatctaata gaaatgttat ttcctataat gtttatactg atttttataa
     4141 taattgtttt cacttttgtg aaaggaatag ctacttggtt taaaaacaat aattctcctc
     4201 gcttaactgt gtcagcaaga atagtagcaa aacgtcagaa taccactcat aacaatcaac
     4261 caaacgctgg tgatatatca ggagcacatg gatttcatac aatttccagc acctcgtatt
     4321 atgtgacatt tcaagtagat agtggtgata gaatagagct gtctgtatct ggctcagagt
     4381 atggagtgct tgcagaggga gataaaggac agctgacatt ccaaggcaca agatatttaa
     4441 catttgatag ggaaaattga atttagaggt gcattatgaa taagtttttg agtcagataa
     4501 ggcgtataga taatgaaata tctattacta ggaaaattat aaatacaata tttatactgt
     4561 gttttggaat agttttagga acttttgcaa aatttttaga tactacagca agcaatactt
     4621 taccatttat atttgaatat ttagatatta gtaatttttt tggtcgtttt gctatatggt
     4681 tattgattgc tttgtacatt gcaatttata gtcattcttc cataagagca tcattaaatg
     4741 tgttagtatt tttcattgga atggtttcaa gttattacct atattcgtat tttgttgctg
     4801 gattttttcc aaaaaattat gcaatgattt ggcttggttt tactgtcatt tctccattgt
     4861 tagcttttat ttgttggtat gctaagggga aaagcaaaat atcttttata ctttcagtaa
     4921 taataattgc aattttattt aattttacat ttatttatgg ttggatttat tttgatgtgt
     4981 attcaatttt agaagtgata gtctttggtt gtgcattaat agctttaaaa cgtaacacct
     5041 ttagggagac aacgtatatg atattgagtg cagttgttat tgctgttata cttaatatgt
     5101 tagttccatt tcattttggt taggcagatt ttaagattat tatagataga atataaattt
     5161 cacgaaccgc cgaagcgatc cgccagcggt ggcggtcgta agacatatcg ccacgccagc
     5221 ttgctggcga ggtggcggtt cactttggcg gtttggcgag cctgcgagct ttctaagccc
     5281 ccgttatcta acaggggggg actattacaa aagacaagag caaaaacaaa tataaaactg
     5341 ctgatatgca acgatttata aggttgtaag aacttatttc actttacacc ctattctttc
     5401 tacccgacag gtctaaaaca gcatatcaat agaaaataaa tataagatac aaggtgtatg
     5461 ctcctatgag tatatgcctt tttttgattg gaggaatgaa gcatgaatga tatgaaattt
     5521 attgaaacat taaagcaaaa gagaaatgtc tgtgattatt cgcaatcacg actggcacag
     5581 gaactacaaa tcaacaggca gaacttaaat gaaattgaaa acggaaaaac aaaagccagt
     5641 aaagaaatga aacatatact cttacattat cttgattatt gtaattgtac acagcctttc
     5701 actttaacga ttgactatct gcgtgttcgt tttcctacca cagacgcatt ggaaattata
     5761 aaaaatgtgc tggcaatgaa aagcgaatat tttatacatg aagattacgg aatgtatgct
     5821 tatgaagaac agtatatcta tggggatatt agtgtaaatg cttctaaaga tagttctatg
     5881 ggtgttttat tggaattaag aggtatgggg tgtcgggact tagaatatgt tttacaggta
     5941 agagggatag actggtattg ctttttaaga tgttgtatag attatcaagg tgtttttaaa
     6001 cgcatagatt tagcagtcaa tgatatgggc ggattgctgg atatagaaat tctaagggaa
     6061 cgttattatg ctaacaaggt atggaaacgt tcaagaaccc atgaagcagt agacagcgga
     6121 aaattatcgg gtacacatgg agatactgca aaaacttttt atatcggttc aaagaatagt
     6181 tctatctact tttgcttgta tgaaaaagaa aaggaacaaa aaagcaaggg tataaaaaca
     6241 gatattaaga accgcttcga aattcgttta aaaaatggaa aagcaggaca aacggtagag
     6301 caacttgttt tttcaagaaa tcccgaacag accatagcaa gctttattct cacacagata
     6361 gattttcccg attatatttt atgggatata tttttagata atgtaacgac ctcacttccg
     6421 tttattatga cacctgttgc tgttaatatg gataaaataa aacgctggct agaaagacag
     6481 gtcatgccct ctttattgat gataaaagag attgaaaaga aaacaggagc aaattatctg
     6541 gaagaaatcg acaggcatac aaggctaaca gaaaagcagt aattaaagat taaacaaatg
     6601 acaacagaca tagcagatat gattgagaaa gataccgctg ttccacaagg gaatgacggt
     6661 attttttaat ttcttcaaaa aaactgcaac aaaatgactg cttgtgtaca gatatggtgc
     6721 gaggaaagga aatctaactt tttttgaaaa caggtcatta aatcggtggc tgctgtccct
     6781 atatgatgtg aaagaagaaa cgatttttaa tttcaactta acatttgaca gttttcattc
     6841 cctatatgga gcaaagaaaa tcttttattc catagttggc agttacggtg ttgcatgggt
     6901 agttagggtg tgaaaagaaa aacagatttt tttcatcacc aacttagcaa actgatactt
     6961 gctttcccta tatggtgcag gaaaagagag tttaactatt ttcatttcaa cttagcaact
     7021 ggacgtttgc tgtacagata tgtagtgaaa agagaaaaag ttttgaatat tggttacgct
     7081 tcggctcttt tccaacgcgg tatataggaa agacgatttt cttctttcat gttctttgac
     7141 aactgaataa agcaattcaa tacgtttgac agacagcgat ccattgaagc agatacgcca
     7201 tgaccttttc cctgcaaaag cgagcgaacc ctatttgtgt tcctgtaaaa gacttgtcct
     7261 cttttatcgc catgacctgt tctgtctgat aaatgatact cccgtacagc cacagcttga
     7321 acgttcagag cgtcgcacgc aatgggtacg gctacataag aactatgcag aggtggaatt
     7381 cctgtgggct atgacaaatg ccgttgaatt gcttagaaaa aagattatgg aaagggggta
     7441 tgtgttattg ctgatattgt aaaaacaatg caagaaaaga caaataataa gcgtagcatt
     7501 ggcaacagag gaaaaacaaa gattgttgta aaagagcatt tttccgaaaa gggaaagaca
     7561 atggaggaac ttctgactga tgttatgctg gaaaaagcaa agcagacaat cgcataaaat
     7621 tggtgcagtt gtaagacata gattgaatgg caaaaagtac ccgtgttata ctttacttgc
     7681 aagcaggtat tgtaaacacg ggttagttat aaaggaggat aactgacaat gaaacaacag
     7741 atttacaata ctgcacttta tcttaggtta agccgagatg atgaattaca gggcgaaagt
     7801 tccagcatta ccacacaaag aagtatgttg cgtctatatg caaaagaaca tcatttgaat
     7861 gtgattgatg aatatattga tgacggctgg tcgggaacaa attttgaaag accgagtttt
     7921 caaagaatga ttgaggatat agaggcagga aaaatcaact gtgttgtaac gaaagacctt
     7981 tcccgacttg gcaggaacta tattatgaca ggacaatata cagaattgta ttttcccagc
     8041 cataatgtcc gttacatagc gattgacgac ggtgtagaca gcgagaaagg cgaaagtgag
     8101 attgcaccat ttaagaacat catcaatgaa tgggtggcaa gagatacaag ccgtaaagtg
     8161 aaatcggcat ttaagacgaa atttgcagag ggtgctcatt atggtgctta tgctccatta
     8221 ggatataaga aacaccctga catcaaagga aaactgctgg ttgatgatga aacaaaatgg
     8281 attgttgaaa aaatcttttc cctagcctat caaggttatg gtagtgccaa aatcacaaag
     8341 gtactgcgag aggaaaaagt tccgacagcg tcttggctga attttacaag gtacggtact
     8401 tttgcacata tctttgaggg aaagcccgaa agcaaacgtt atgagtggac gattgctcat
     8461 gtcaaggcaa tcttaaaaag tgaggtttat ataggcaaca gcgttcacaa tatgcagtct
     8521 acggtttcat tcaagagcaa gaagaaagtg cgtaagcccg aaagtgaatg gtttcgagtg
     8581 gaaaataccc atgaaccgat tattgacaaa gaagtgttct atcgtgtgca ggaacagata
     8641 aaatcaagac gtagacagac aaaggaaaaa gcaacaccga tatttgcagg gcttgtcaag
     8701 tgtgcggatt gtggctggtc tatgcggttt gcaacgaata cggcaaataa aacgccttac
     8761 agttattatg cttgcagtta ctacgggcag tttggcaaag gtacttgttc tatgcactat
     8821 atccgttatg atgtgttgta tcaagccgta ttgaaacgat tgcagtattg ggctaaggca
     8881 gtacagcagg acgaagaaaa ggtgctgaac aagatacaga aagcaggcaa tgcagagcga
     8941 atacgggaaa agaagaaaaa ggcaagtgcc tttaagaaag ccgagaaccg acaaaatgaa
     9001 attgaccgtt tgtttgcgaa aatgtatgaa gatagagcct gtgagaagat aacagagcga
     9061 aattttgtga tgttgtctag caagtaccaa aaagaacaaa tagaactgga acagcagata
     9121 accaacctaa gagaagaatt aagtaaaatg gaacaggata tgataggtgc tgaaaagtgg
     9181 attgagttaa tcaaggagta ttccgtacca aaggaactga cagcaccgtt attaaatgca
     9241 atgatagaaa aaatccttat tcatgaagca ataacgaatg aggataacga aagaatacag
     9301 gaaatagaga tatattaccg atttattgga aaagtagact gataaagagg gttcatatct
     9361 ttaactaagg gaaactggag aatctattcc tgatataggt agttgcatta aacttgcaaa
     9421 attgtacaag gtcaaactag atgacttagt taatcattcg gaagaaaaaa caggtattat
     9481 agtacctcct aaaggaaagt ttttctttgg agcagtagta gttggtgaac gtggtcagat
     9541 tgtcattcca aaagaagcaa gagaagtatt taatataaat gcaggagata aattattggt
     9601 acttggagat gaggaaagag gattaggaat tgttcatcaa cgtgatttga taaattttat
     9661 aggtcatatg ggagtagtac gtaaagaaga atgattttaa aaaattataa aaggaggtat
     9721 gaaatgtgag taaaatagta ttttttagca ttcttgcata tggtcatact aatcctacta
     9781 ttgaagtagt tcgtgaatta gtggatagag gaaatgaggt attgtattat tcttttaatg
     9841 aatttaaaga taaaattgaa ggtgctggag caaaatttat ttgttgtgat aaataccttc
     9901 cagaattact ccctggagat gagaaaaaaa ttggtaagga ttttcctgga ttaattgaga
     9961 tgatagtaga tactactatt tctttagatg aaaaagtttg tagggaactt aaagatttta
    10021 atccagattg cattgtatca gattcattaa gtttttgggg aaagttattt gcacaaaaac
    10081 ttaatattcc atatgtatgc tctacaacaa cttttgcatt taataaacat acagcaaagt
    10141 tgatgaaaca aaactttata gaaattatac gtatgatttt tggtgtaaga cgcattaata
    10201 aaaaaataaa gcttttacaa agaaaagggt atgaagtaaa aaattttatt tctatagttt
    10261 ctaatgataa tgatacggat accattgtat atacatcaaa agaatttcaa ccaatggtag
    10321 aaaccttctc tagtaagtat tcatttgtag gtccatcagt atcaaagttg attattaaac
    10381 ctaaagagag aaaaagaaag ttaatatata tttcgcttgg tacaattaat aataaaaatg
    10441 ttagctttta taaaaactgt ataaatgcat ttaaagattc tgatgttgat gtgattatgt
    10501 ctgttggtag aagtactgat attaaaagtt taggaaatat accaaataat tttgaggtta
    10561 aaaatagtgt tgaacagatt gctatacttc aaaaaactga tgtgtttatt actcattctg
    10621 ggatgaatag tgttaatgaa agtttatatt atggagtacc catggtatta tttcctcaac
    10681 attcggagca gagaatggta gctaagagag ttgttcattt gggagcagga attatgttaa
    10741 aagaggataa gtccgaaagt attaaaaaag cagtatttca agttataaat gacaatgaat
    10801 ataaagaaaa tgcaactaaa ctatcaaaaa gcttttataa tgcaggaggc tcaaaaaaag
    10861 cagcagatgt aatattacag attatatgta atagtagaca agttgacata taatagattt
    10921 gttaaaaata tatatacata catgcaattt aatcaaagta ctgacagtgg ggtcttttaa
    10981 cctaccagtt ggggcatata tattgatgct aaatatgagg gatgttatat tttaataaaa
    11041 aagataacat ccttttttta gtgcgttata tcaagtttta aataataaat gaaggaggat
    11101 taatatgaaa aaatataaag ttatttttat agtattaatt ataggtatta taagtgtaac
    11161 agggtttgtt gtatataaaa actgcattaa accatggaaa gatgctgaaa catatattga
    11221 taagtatatg gtaaaacaag atatttcaaa agataatata aaaagtatta caaaagaaaa
    11281 agccaagaag gcaagttacg aaggaatact atacaaagtg tattacaaag atgaccctcg
    11341 atatgagtat caatattttt atagtgataa ttatcatgct ctatatgtaa ataaagtaat
    11401 gttacaaatt tatgatgtgg aaaataataa taaactttta agaagtaatg aggaattaaa
    11461 aagtgtaaag tatccaccaa tatatcttaa atataagcca gataaatgat ttatgacaaa
    11521 atagaatgat ttttattaaa atttatttaa aaatatcact agagtgttta aataaaacta
    11581 aatttaagta tttatatatt agtatatctt aatagcattt aaataaaact aaatttgaag
    11641 ctttacctat taatatatct taatagtgat taaataaaac taaacttaag aatttattta
    11701 ttaacataca gttcattata tttaaaatca ttactatgat aattcataat agtaaaatag
    11761 ataatggttc agacaatatt gtaaatatta gtgacagtaa aatagtatca atttagtata
    11821 taaactttgc cagataatcg cataacagag cttgtagtta tagaataaaa aaatatctag
    11881 gaaaccgttg acagatttat atggatagta taacatatag aatatgaaat taaacaaaaa
    11941 agaattttaa ttatggcgac gtagtcataa ttttgagtat gttactgatt agcaggcaga
    12001 actcaagtat atatgtgaaa aatagaggga tactctatac atatatgctt gagttctttt
    12061 tattgaaagg aggaatatat gaaaataatt cagattttaa ataataatgt agccttagta
    12121 aaaagaggta aaaatgaaat tattgtattt gttaaaggaa ttggctttaa aaaaagagtg
    12181 ggacaagtaa taaatgaaaa tgaaatagag agaacatata tactagattc atacgatatg
    12241 ctagaacact ttagttatct actacaaaac tcagactcca ataatatagt tcttattaat
    12301 gaaataattg aatatggtga gaaagcttta aatataaagt caagtgatta tctaagctta
    12361 accttactag accatttaga gtttttactt aaaagatgtg aaaagaatca atttataaaa
    12421 agtcctttgg tatggaatgt taaaagattt tatccaaagc attttgaaat tggaatgcat
    12481 gcactaaaat taattggtga tagtaaggga attaatcttc ctgatgatga ggcagtatca
    12541 atagcacttc attttgtaaa tatggaggta aataaagagt ctcatgatag tacaattgta
    12601 gagttgagaa ctttagcaga tatagtttct ataataaaat atcattttaa tgtagagcta
    12661 gatgaaactt caacaaatta tatgagattt actactcatc tacagtactt tatccaaaga
    12721 attatgtcag atgaagcttc aatagatgaa gataattcta tagatttata taaacaagta
    12781 agtcaacttt atccaaagtc atttggaagt gttcaaaaga taagaaaata tataaaatca
    12841 caatttaatg tcgatatatc tataagtgaa gaaacttatt taatgttgca tataaatagg
    12901 gtgactcaaa gactggaggc aaaatcagat gaaatataaa gatttaaatg aaaatataat
    12961 aaaacttata gggggaaaag aaaatataaa gtcggtagcg cattgtgtta ctcgtttaag
    13021 acttacttta aatgatagaa gtatcgctca aacagaagaa attaaaaaat taaaaggagt
    13081 aattgatgta gtttctaatg atgttgctta tcaaattata ataggtactc atgttgtaga
    13141 tgtatataac gagtttatgt ctatgatagg tttaagttca agtgaatcaa gtataaaaag
    13201 tgaaagaaaa aagggaatta gaggtatcaa aggtatattt aattccatct ttgtggtagt
    13261 ctcagagact atgacttcag taattgaagt actacttgca gctggtatac tcgcaagtat
    13321 attagcactt ttaaatatgt ctggcatact tcagagtgat agccctacat atcaaatatt
    13381 agatacatta agaagtgcta catttcactt cttaccagta ttaattgcaa tagcatcagc
    13441 aaaaagactt aatgtaaatc cctatatagc agtagtgtta gcagttacgt tattatcgtc
    13501 atcaatagat ggtgtagaag gattaagttt atttggtata aatttacagg ctacaactta
    13561 tgcaaacagt tttattccaa tacttttagg agtatgggca atgggaatta taattgaagg
    13621 tttgaaaaaa atattaccta aagcactaca ctatttttta gttccagtgt tatctttaat
    13681 aataacccta tcagttacat taactgtatt tggacctatt ggtatgtgga ttggtgatgg
    13741 attaaactat gcatgtatgt ttttaattga tacattgggt aattggagtg ttgttgcact
    13801 gtatgcagct ttacaaccac tcttaatagt ttttggtgca gcaaacttta ctatgcctat
    13861 atctttaaat tctgtgacaa cattggggta tgacccaatt tttatgggcg ctgcaacaat
    13921 atctgatatt gcagtttgtg gtgcaatgtt tggatatttc ttaaaaacta ggaataaaga
    13981 acaaaaacaa ttatttggga cagtaagttt tagtgcactt atgggagtta cagagccttc
    14041 agtgtttggt gtgtttatga aatatagaag accatttgta gcagtaatga taggtggagg
    14101 tataggtggt ttaatagcag gtttagctca agttaaaaca tatggaatgg tttggggatt
    14161 agctgcatta ccaacatatc ttgttggagg agaggcatct aactttacat ttatgataat
    14221 aagtgtagta gtatcatttg tagcagcaac agcagcttcc tatttactag gaataccttc
    14281 agatgaaaaa gaagaagtta agggtgataa tgaaaaagaa ttagaactaa taatcaacga
    14341 gaacaattca agaactatta gtattggaaa tgttgcaaaa ggtcaggtaa ttgcacttgg
    14401 ggaagttaat gacaaagcat tttcctctgg agcattaggt aaaggggttg gagttatacc
    14461 tagtgaatca aaatcaactg ttatatctcc tgtagatgga actataacta tagtatttcc
    14521 aactaaacat gcatatggta tagtgactga tgaaggatta gaaatattaa ttcatattgg
    14581 tatagatact gtaaacttag atggaaaatt ctttgaaagt cttgtaacac aaaatcaaag
    14641 ggttaaaaaa ggagatgctt tagcagtatt tgaatctgat aaaattatag aggaaggctt
    14701 tgattctact ataataactg tagttacaaa tactagcgat tatttagatg taatctcatc
    14761 aaatgatgaa tcagaagaat tattaactgt aatagtataa attttaggag ggaaaattca
    14821 tgaatattaa agatacattt ttctggggag gaagtattgc agcccatcaa tgtgaaggtt
    14881 catgggatag tgataacaaa ggacctgcaa ttatggattt tgtaactaag ggctcttatg
    14941 aaacgccaag agtaattact gataaaatag aagaaaaatt ggattatcca tctcataatg
    15001 gaatagattt ttataataga tataaagaag atatagcttt atttaaagaa atgggattta
    15061 gtgcattaag aatatctata gattggtcaa gaatttttcc aaatggagat gatgaaaatc
    15121 ctaatgaatt agggattaaa tactatgaag gtctgataga tacattaata gaaaataata
    15181 tagaacctat tgtaacacta tatcattttg aattacctat gaatttagta cataaatatg
    15241 gttcatggaa taacagaaaa cttatggatt tgtatttaaa gtacagtgag actgtaataa
    15301 gaaggtttga tgataaagta aagtactgga ttacatttaa tgagatgaac catattgacc
    15361 ctcaaacaga gcactcggat atttttactt atatgatagc aggtattaag tatagtgaaa
    15421 tagaaaataa ggttcaaaca ttggcaaatg tagggtataa tatgactttg gcgggtgtaa
    15481 aggttgcaaa gttggctaga gaaatcaatc ccaacaataa agttggatgt gtatttggac
    15541 taaatccaat ttatccatac aattgtaatc ctagtaatgt gttaaatgca tttttagata
    15601 atgatcgtga ttattatcaa attgatgcaa tgtgcaatgg gaagtttccc caatataaat
    15661 taaaagaata tgaaaaaaat aatataacta ttgaagtgac tgatgaagac aaaaaagcat
    15721 ttaaagaagg taagttagat tttataggtc ttaattatta tttttcaagt gtatctactc
    15781 caaaagaaaa tttatcaggt gataaatctt tatttggtgg gattcaaaat ccatatttgg
    15841 aacaaagtaa gtggggatgg gcaatagacc ctataggttt aagatttacc ttgaactata
    15901 tttacagaaa gtatcaatta cctatcttaa ttacagaaaa tggtttaggg gcatatgata
    15961 aaatcgaaga agatggaact ataaatgatg attatagaat tgaatattta agtaagcatc
    16021 tatctcaaat gaagtctgca attgaagagg ataatgttga atgttttgga tatttgatgt
    16081 ggggaccaat tgatttagtc agtgcaacta caggtcagat gagtaagaga tatggattta
    16141 tatatgttga cttagatgat gaaggcaaag gaagttttaa gagatataag aaaaaatcat
    16201 ttgattggta caaagaagta ataaaatcta atggagaagt tttagatttt tctacggata
    16261 tagtagtagg gtagtaataa gataaataga agttaggaac taatgttaat tagtgtctag
    16321 taaagcattt aaaatagagt atagggtctt taaagtaaag tgtgtattct tattaaaagt
    16381 aatggcaact aaaattacta taaaaaggag aatacacatt ttttatgttt atacttaagt
    16441 aaatatttag acatattcat agtataattt actcattata taaaaatagt atgatttact
    16501 cactatataa ctttataaat tttctagaaa aatgtcagaa tatattgaca aaaaaaagat
    16561 ttgaaattat aatctatatt gagaatagtt tgacaatata aaaacaagta tttttacaaa
    16621 tagtgaaatt atttttattg tcaatttaag aataatttag gactactata aagatgaagg
    16681 tatagatgtc attatatgat aaatcatact tgtatagaaa gaattttaaa gtatgcttat
    16741 cattgattta atataataaa taatttttgt ttctagtaat agttcatagg aataagatat
    16801 ctaaattcgc ttagaaatag tctaatttac aaaggagggg atgcactagt agataaaatt
    16861 tttatataaa gaagacttaa agtggagtat attctgataa tattggttta atggttatat
    16921 tattatttcg ttttagatat tttaagaata atttaagacg tttgtaaaaa tgtattctat
    16981 atctaatatt taaataaata taagtcttcc aacatgtata ctaagataga aagtagttga
    17041 agtacaattc taacttgtaa aattatgtag aaagtaaaaa tcaaaaagaa atctagtaag
    17101 ttgataatta tagttaatat ttagagagtt aactctagtt atatttattt agatatttta
    17161 ggaataattt aagacgtata taatatcatc aaaaaatatc aagctatacc atatgtagtt
    17221 aagaataact gcataatata cattttataa ttattgtttt attgataaaa aattattata
    17281 tttaggagga aacaacaatg gaaaattttt ggaatccact tacttgcttt actgtatgtg
    17341 taatgattta tgcttttagt gagtttttat ctaagaaaac aaaaggtgca atttcaacat
    17401 tgttatttgt atgtattata tttttactag gattttggac aaatgtttta ccaaaagaca
    17461 ttacaggagc atcagggata ttagctgcaa tgagcaactt tggtatagct ttattaataa
    17521 ctaacttagg aacacttata aatcttgaag acttatgtca tgaatggaaa acggttgtaa
    17581 ttgcattagt aggtattgta ggaatagggg ttggctgttt aacagtgggt agtatgttat
    17641 ttggtagaga gtatgcttta atagcagcac ctccaatagc aggttctact gttgccggta
    17701 ttatcgtaac aactgttgca gaagctgcaa atagacctga acttgctgca tttgtaattc
    17761 tagtattatc atttcaaaag ttctttggca ttccgataac tacatattgt ataaataaag
    17821 aattaaaatt taaaagagat aacggggatt ttttgaaaga tgagtcaaaa agtaatttta
    17881 gacttccaag tatgagaatt tttaaagaag atttaggtaa aaaatcatct actctagtgt
    17941 atcttgcaaa gctttcattt gttgctatga ttggtaattt tgtaggattg gctactttaa
    18001 taccaggatt tacccctgca aactattatt taaatcctaa tatagcatgt ttgttagtag
    18061 ggttgatttt tacacgaata ggttttttag aaaaaggaat tttagataaa tctcaatcta
    18121 atggaattat aatgtttggt tgtatgttga tgttacctgg aggacttgct aaggtaactc
    18181 caacagcatt gtttgggatg attgttccaa ctataggaat acttgttgta ggttctgtat
    18241 ttataatatt atttactgca attgtaggaa aactattggg atattctcca agaatgtcag
    18301 ctgcaattgg tgtaacttgt atgctagctt atcctgcaac acagataatt tccaacgaag
    18361 gtattgatgc tatggaagga actgaagaag aaaaacaaag ggcaacagat tatttattac
    18421 caaagatgat tataggagga tttgttactg taaccattgc atcagtggca tttgctagta
    18481 taataggtcc aattatattt ggttgatagt aaatatactt ataggaggaa taagaaatga
    18541 taggaagtat atctataaat gagatagata aaaaaagatt caaacttgat gatttaagta
    18601 agaaaatatg ggaaaaccca gaaaaagcat ttaaagaatt taaagcatgt gaaaatacag
    18661 caaactttct aagaagtgaa ggatttgatg tggaagtagg tgtaggaggt ttagccacgg
    18721 ctattagagc tagttttggt tcaggtaaac cagttatagg ttttatggga gaatttgatg
    18781 cattacctgg tttaaatcaa aaagtttcaa caaagcaaga agcatttgaa ttgagtgcat
    18841 atggtcatgg atgtgggcat aatttattat gtacagcaca tgtaggggca gtagtaggac
    18901 ttaaaagaga gatgctagaa aataacttaa gtgggactat agtattttat gcatgtccag
    18961 gagaagaaca attaacaggt aaaggattta tggctagagg tggagcattt gaagggttag
    19021 acttagctat aaattttcat ccaaataaaa ttagtgaagc tacaattggt acttcaacag
    19081 ctgttaactc tgtaaaattt cactttaagg gaaagactgc acatgcaggc tcagaccctc
    19141 aaaatggaag aagtgcacta gatgctgtgg aacttactaa tgtgggagct aattatttga
    19201 gagaacatgt tacttctgat gtaaggattc attatacaat tacagatgga ggagtagcac
    19261 ccaacatagt tcctgataaa gcatcagtat ggtattatac aagagcttta agtagagaag
    19321 ctgtagaaag tacatatgaa agacttgtaa aggtagcaaa aggagctgca atgatgactg
    19381 aaactgaagt tgaagtagag tttttaggag gttgttataa tactttaaat aatcatgttt
    19441 tagcaaatct tgtatcagaa tgtatgaatg agattaaaca agagccttgg actcgagagg
    19501 aattggattt tgcagctgaa ttagatagac aaagcccaca acaatataag gctatgataa
    19561 gtaaatataa tttgccagaa ggaactcatc tatattatgg tggaggaaat gttacaaact
    19621 ttaatagtta tggttctact gatattggag atgtaatgca tatagttccg acagcatact
    19681 tttttacagg gtgtacgaat ctaggtgcac caggtcatag ctggcagttt acatcttgtg
    19741 caggaagttc tataggagaa aaaggtatga tatatgcatc aaaaatcatg gctatgtttg
    19801 gagccaaaat attaaataat ccagagatag ctaaaaaggc aaaagaagag tttgataaat
    19861 ctatgaatgg taaaacatat aaatgtccta ttccagatga tattccaact ccttaaaaag
    19921 acatgaagtt taatatataa aattataatt tttatatcag catcatttca ttactaaaag
    19981 aaagtgagat tttgctgact atctgattag gaggaaggaa tatgaaaaat ttttttaaag
    20041 aagcgcagtc aatagaaggg gaaattatta gatggagaag atatttacat gaaaatcccg
    20101 aaattggttg caatctaccg aaaacagtaa cctttgttac cagtacttta gaaaaaatgg
    20161 gatattctcc gaaattgacg tctaataatg gaatagtagc tgttttagac agtgaaaatg
    20221 gtggaaagac cttattatta agggcagata tggatgcact tcctattatg gaggagacgg
    20281 atttaccttt taaaagtaag aataaagatg catctcatag ttgtggacac gataatcaca
    20341 cagcaatgct cttaggagca gctaaaatac ttatggataa taagcactta cttaaaggta
    20401 gagtaaagtt tgtatttgaa ccagatgaag agagtaccag aggtgcaaaa tcaatgattg
    20461 atgatggtat attagaaaat cctcaggtcg atgcagctat ggcatttcac agtatggtgg
    20521 gtaaatttct taacacaggt caagtagctt attcacgtgg tccagctatg gcatctgcgg
    20581 atatatttga aataacagta gaaggaaaag caactcatgg agcaagccct gaatttggag
    20641 tggacccaat aaacataatg tcgcatatac aaatagcatt aaacactata ctttcaagag
    20701 aaaaacctca gaaagagcct gtagttttaa ctattggtat gtttaatgca ggaaacgtac
    20761 caaatgctat accaaataaa gcatctatgt caggaacttt aagaacattt gaccaatttg
    20821 ttagagaaaa attaaaaaat agaattattg aaatatctac aggaatagct aaatcatttg
    20881 gaggaaatgc taaagtggaa tttaaaatgg ggactcccgc agttataaat gatttggatg
    20941 taggagatga gattgtatct tatgttaaag aaattatagg aaatgagaat gtaatagact
    21001 ttccatctgc aatgggttca gaagattttt cagagatttt actaagggtt ccaggagtat
    21061 ttttatggat tggaatggga agtattagtc agggatataa ttttggtatg cataatccta
    21121 aagcaatttt taatgaagag ggattagttt atggttcagc tttatttgct cattgtgcag
    21181 taaaatggtt agaaaataat tttagtagat aataacataa aatattatta aatatcataa
    21241 aaggtatatc ttaaaaagat gcaaagatat accttttaga aagtcatata taattttact
    21301 taatattaga tgataaaata tttttatgca ttttggattt taatatttat tttctgatat
    21361 aaataatttg tatacccttt caggtctacc ttttgttgag ttttgttttt catataatat
    21421 agtagcgttt ccagacttta agagactact tagaaatcta ttagcagttc taactgttac
    21481 atttaaacat gaagataatt ccttaggagt aacttcatct ctttgtagaa tttcagtaac
    21541 agatataatc ttttgaatag taagagtaga caatttagaa cttttggcaa ttgtatttat
    21601 atgtggagtt actttatttg aaacagtaag taacttatca cacagtaaag gtcctattgt
    21661 ctcataattt tcgttgacta agaaactgta atttaaagga tttattttag attccttatt
    21721 agcacttatg gcactagaca tagcttgctt tatatcatta ccaattccat atccaacaca
    21781 tactttaata tttgtactat tttcaataga ttcttttata taacatgcag taaaattttt
    21841 agttaatgat ttaatcgttt cacatttagt gaaaatttta aaagaattac tatttttttc
    21901 aattataaaa tcggttaatt tatctttttt aatactattt aatattttat aaacttggtc
    21961 tagagtagag tcatcataat ttgtatcttt aactgttata tatattacac ttggttgatt
    22021 tcccttcatt ctatgtaaat gaatatcttt tattaagtta ttaaaagtat tttttaataa
    22081 atctatatca gggtatatga aatgaaagtt tactcctgca ttttgtaaca taggaacaat
    22141 actactaaaa cgagtagtag aaaaatcgat tttcccttca ttccatagat taatatgttt
    22201 ttttgcaatc tttttttcaa gttttgctat gtcagataaa ctcattttag atgcattttc
    22261 atcaatacta tccattaagt ctccaaatgt gccatttgat aaataatatg atatatcttt
    22321 tggattatct gcccagtctg ttaagtcaaa gtatgctcta gaaaaatcta atgtattata
    22381 tttaaagata agctttagaa acatatcata gtaactttgt aagtcagtac caaactgaac
    22441 ctcccatgac taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc
    22501 ttactaattc t
//
LOCUS       DAEPOG010000049        22454 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEPOG010000049 DAEPOG010000000
VERSION     DAEPOG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.049"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..259)
                     /locus_tag="KR432_001585"
     CDS             complement(<1..259)
                     /locus_tag="KR432_001585"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH0640127.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIE"
     gene            355..848
                     /locus_tag="KR432_001586"
                     /pseudo
     CDS             355..848
                     /locus_tag="KR432_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1390..2745
                     /locus_tag="KR432_001587"
     CDS             1390..2745
                     /locus_tag="KR432_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0640128.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2863..3531)
                     /locus_tag="KR432_001588"
     CDS             complement(2863..3531)
                     /locus_tag="KR432_001588"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBH0640129.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3598..4026)
                     /locus_tag="KR432_001589"
     CDS             complement(3598..4026)
                     /locus_tag="KR432_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0640130.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4133..4726
                     /locus_tag="KR432_001590"
     CDS             4133..4726
                     /locus_tag="KR432_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBH0640131.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            4846..5781
                     /locus_tag="KR432_001591"
     CDS             4846..5781
                     /locus_tag="KR432_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH0640132.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6052..6996)
                     /locus_tag="KR432_001592"
     CDS             complement(6052..6996)
                     /locus_tag="KR432_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0640133.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            7312..8742
                     /locus_tag="KR432_001593"
     CDS             7312..8742
                     /locus_tag="KR432_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640134.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            8942..9526
                     /locus_tag="KR432_001594"
     CDS             8942..9526
                     /locus_tag="KR432_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBH0640135.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(9630..10409)
                     /locus_tag="KR432_001595"
     CDS             complement(9630..10409)
                     /locus_tag="KR432_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0640136.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(10479..11735)
                     /locus_tag="KR432_001596"
     CDS             complement(10479..11735)
                     /locus_tag="KR432_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBH0640137.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            11993..12892
                     /locus_tag="KR432_001597"
     CDS             11993..12892
                     /locus_tag="KR432_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0640138.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            12916..14364
                     /gene="ddlR"
                     /locus_tag="KR432_001598"
     CDS             12916..14364
                     /gene="ddlR"
                     /locus_tag="KR432_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBH0640139.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            14423..15229
                     /locus_tag="KR432_001599"
     CDS             14423..15229
                     /locus_tag="KR432_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0640140.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            15255..17174
                     /locus_tag="KR432_001600"
     CDS             15255..17174
                     /locus_tag="KR432_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0640141.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(17416..17763)
                     /locus_tag="KR432_001601"
     CDS             complement(17416..17763)
                     /locus_tag="KR432_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBH0640142.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(17750..19678)
                     /locus_tag="KR432_001602"
     CDS             complement(17750..19678)
                     /locus_tag="KR432_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBH0640143.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            complement(20089..22278)
                     /locus_tag="KR432_001603"
     CDS             complement(20089..22278)
                     /locus_tag="KR432_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBH0640144.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT         8212 a         3357 c         2863 g         8022 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga
     1021 ataaatattc gatattctca cttattagat gaaatggata aagaaacatc tgaaaatata
     1081 agcaaaatac tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct
     1141 ttttttccat ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa
     1201 tgtcagtaat atataaataa gatatattta taagaccctt aaaatcacta ttatttaata
     1261 aaatttataa ttatatacta tttgctgttg acattggtat tataccatag tttatcattt
     1321 atattagaaa taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag
     1381 tgataataaa tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt
     1441 tttaaatacc tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt
     1501 gatggaatat ttgtaagtaa aggagttgga gaattggctt tagctgctgt caatatatca
     1561 atgcctttta ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc
     1621 ataattgcaa tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt
     1681 aacttagtat caataattat attatcaata attatattag ctataacttt ttttaattta
     1741 gatagattag cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat
     1801 ttaggaataa taatattttt taacggattc tttatagtgt cttattcttt agaagttata
     1861 ataaaaactg atggctttcc aatattagct actgttggtg taataatatc tgctcttaca
     1921 aacatcatac ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt
     1981 attgcaactg ttttgtctca agtcttttct actattttct tcttaataca ttttttaaga
     2041 aaaaactcta ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt
     2101 gtatttatag gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt
     2161 tttaacttaa gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata
     2221 aattatataa atacattagt acttatgact atgatgggta taactcaagg tatgcaacca
     2281 cttactagct tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg
     2341 gggataaaag ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca
     2401 ggaccaatag tctctttatt tatacaccca gaagaaacaa tgctattcaa tgaaggagtt
     2461 cgtgtattta aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct
     2521 ggattctttg tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg
     2581 cttgtaatag ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata
     2641 tggatgacta ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa
     2701 aagaattttt ctacattaga ctctaatcta aataaggttg cttagatgat aagagtagca
     2761 atatatgaaa atatctaaat aaaaaatacc tattatacaa tcttgcaaat attagacctt
     2821 tgcaaaaatt aggattatat aataggtatt ttatttatgt aattatttta tttctaaata
     2881 tccatagcca tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat
     2941 tggaccatct acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc
     3001 attttttatc ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa
     3061 gtgctttaag tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac
     3121 taaatcctct ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca
     3181 tctatcagta tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt
     3241 tgctcccatg tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac
     3301 accacactca gcaactaata aaggtaatcc tatattgcta cttacttgct taagcatatt
     3361 atattctaaa ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc
     3421 cacaggctta tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc
     3481 actaaatgac attacaaatt tttcatcagc caattttaac acctctttca tctaactttg
     3541 ttttattaac ataattatac caaatttttg agttgcatag caaattatat taaattgtta
     3601 attcactata aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg
     3661 cattgtttct tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac
     3721 aagtgttaat ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata
     3781 tattattaaa cctccaaata ctcctcctaa taacaactta taattaaaac catttataga
     3841 tactagacac tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac
     3901 agttaaactt ataaaggtgg ccactaatgc tgttgtactt tttcctagtt ctccatttat
     3961 aaaagcttca agagctgtag aaccacctgc taataatgca aatattattg cccataattt
     4021 tgtcatggac aaacctcctt ctatataatt ctattaatag aagtctatga tacaacctat
     4081 taattaatta ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt
     4141 ctgttataat tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga
     4201 ttttaataac tttaataaaa aagaaaatgc tactaaattt atgttaatgg gagtattgct
     4261 cattatctta ggattattat gccttacatt taagacatta ggtataaaat taatttcttg
     4321 gacttttggt attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgaact
     4381 aaaaagatat gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat
     4441 aattgcatgt atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt
     4501 tttagggttt tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc
     4561 ttattctaaa tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt
     4621 atctccactt tttttatcta gatttatagt atcgataatg tcttttttca taatattatt
     4681 tggactagtt ctatttttct ctggaaatac agcgagaaaa tattaattat tttctcgctg
     4741 tatttttata ttacttaaaa tagaatttgt ttaagttatc tatttgtggt aatatgtttt
     4801 ttgtacaagc tcagcgtaca attttattag aaataggtga tttttttgga taaatttaaa
     4861 tatgcttttg atgataaaag atatcatact tggaattact atcttagaaa tacttttgga
     4921 gaaaaagttt ttaaggtatc tataaatgca ggatttacct gcccaaacat tgatggaagt
     4981 cttggttatg gcggatgtac ctactgtagc aaagaaggct caggtgattt tgctggaaac
     5041 cctaaagata atcttatcag tcaattttat aatataaaag aaatgatgtt aaaaaaatgg
     5101 cctcatgcaa agtatattgg ctactttcaa gcctatacaa atacgtatgc tcctcttgaa
     5161 gttttaaaag aaaaatatga gactatatta gaacttgaag atgtcatagg tctttcaata
     5221 tccacaagac ctgactgttt gccagatgat gttgtagaat acttatctga actaaataaa
     5281 agaaccaatt tatgggttga acttggactt caaacaattc atgattctac atctaaaatt
     5341 ataaatagag gtcatgatta taaaacattt gttgaaggtg ttgaaaaatt aaagtctaaa
     5401 aatataaagg ttgtcgtaca cattataaat ggtcttcctg gtgaagatta taatatgatg
     5461 atggaaacag caaaggcagt tggtaaaatg ggtgtagacg gtataaaaat ccatctattg
     5521 catgttataa aagatactcc tatggaaaag atgttacaaa atggtatgtt aactcttatg
     5581 gaacaagatg aatatatcaa tcttgtctgt gaccaattag aaatacttcc tgaaactatg
     5641 ataatccata gactcactgg tgatggtaag agggatgaac tagtaggtcc tatatggagc
     5701 ctaaaaaaat gggaaatttt aaatcaaatt gatgatactt taaaggctag aaattcttat
     5761 cagggctgta agtttgttta atacaaaata ataactatag aaatggttat ataaatatag
     5821 tcctttctat agttatttag tatttacaaa acaaaatatt aatttgtaca tttatacaat
     5881 atataataaa aaatagatag tctaaactgt atctacttat attttatttg tactgtccac
     5941 tttattctat aaaatttata aatagttatt ttttaatacc aaatataatt tggtataatt
     6001 tttgttggat tctaaaatgt ataccaataa atccttttta agtatataat tctaaatact
     6061 ttcagttttt tcaatctgct ttaacttgtt ggatttataa aatattctta tttcatcata
     6121 agaaagatta agtgctacta ctgcagatat taacatcaac atcactccaa ataacttccc
     6181 taaggaaaaa ctttctccta atatagtgca accaattatt actgaaccaa ctaactctac
     6241 tgatgccaca acacctgcaa ctgacaactc tacaccttta gatatacctg ctgcataaaa
     6301 aatatatgca agtgcagctg gaacaatgcc taacattagc atacaagata aaacatctaa
     6361 atccttagca taatttaaga tttcccaagg tctcgaagaa ggaatcataa atatagctcc
     6421 aaataagaaa ctgtatacta atattgttga acttgaaaat tcctttaaag catttttact
     6481 aatagttggc ataagtgcat atgctattgc tgataatacc ccaagtaaaa gtccaaaagc
     6541 gtttaaacct tgaaaatcca aacgtccacc agtcactgcc ataattgcac caataaaaca
     6601 aagaataagt gaaaataatt tgcttctagt aatattttct ttataacata cttttgaaaa
     6661 tattgctaaa aacagtggtg atgtatatag taaaactgct gcaattgata cacctgcaat
     6721 gtcaattgct ttaaaataac ataaattaaa cattgcctga catataattc ctattattac
     6781 tgaatatata attccttttt tgcttatctt taagatttgt ggtgtcctta tagatgaata
     6841 tacgcctaaa actataaatc caaaaaacaa tcttgtaaat gctacttgtt ctgatgtcag
     6901 tccatttccc attaaaaact ttccaaataa tccaagtgta gcccataata accctgctat
     6961 tgctataaat acataccctt tacttttttc tcccatttta aattcccttc tatttttatt
     7021 tagtcaattt acactcttct ttttaagcaa aaattatgcc atcttaaacc aatatatatc
     7081 gactctaaaa ccctagtctt tccaataata ctaataagtt ttcaattttc ttatatttat
     7141 ttttcgctta ctttaagttg atttagactt attttaattg taaaatgagt tatatataac
     7201 ttatttaaag ttaaaataga ctgaaactta tataaatatc aaattttaag tatattaatc
     7261 aattaaacct attttaatat atcattaaaa atttaggagg aaaatataaa aatgaattta
     7321 gatttggaat tttatcaaaa aatattagaa gcttcccatg atgaaatatg tgtttctgat
     7381 gataaaggaa taataatata ttgtaataaa gcatttgaag aaaattatgg tctaaaaaaa
     7441 gaagatattt taggaaaaaa tgtttctttt ttagaagact ctggatattc aacaaaaagc
     7501 cccataccaa ttgtattaaa aacaaagact aaattttcac tggaacaaga tactcaaaca
     7561 gggaaaaagc ttatcataac tgctacccct atatttgatg aaaatggaaa cttagaattt
     7621 actgttgaaa attgtagaga tataactgaa cttaataata taaaaaataa acttgaagac
     7681 acaaaaaagc aagtaaaaaa atataaatct gaggtagaaa cactatacag aactgcactt
     7741 agaattgaag atactgtaat tatggacgga atagtgatga gaccaatcat aaacacagta
     7801 aatcatgttt ctaaaacgga tgtaagtgta cttttattgg gtgaatctgg aacaggaaaa
     7861 agctcacttg ctagatatat acaccacaac agcaatagat ctaatggccc atttataaca
     7921 ataaattgtg caactatatc tccacaactt cttgaatctg aactttttgg atatacttca
     7981 ggtgctttta caggtgcaag tactaaaggt aaagttgggc tcgtagaatt agctaatgga
     8041 ggaactcttt ttttagatga aataggagat ataccacaaa atctacaggc aaaatttttg
     8101 caattaatac aggatagaac ttttactcca gttgggagct taaaaaataa aaaggttgat
     8161 ataagaatta tatctgctac aaatgctgat ttagtttcaa aagtaaaaga aaaaaagttt
     8221 agagaagatt tatactatag attaaatgtt atagaaataa aattgcctcc tcttagagaa
     8281 agaagggata atctagtgga aattataaaa tattatttta ataggtactc aagtgacttc
     8341 aacctaaata aaactatttc taaagaagct atggatgcta ttgcaaatta tagattccca
     8401 ggcaatataa gagaattaca aaatataatt caaaaaatct tgcttacttg tacagataat
     8461 catataacaa tagatgactt accaaacatt cttacaaaaa atatacatat taccaataat
     8521 ggaaataaaa cacatatatc ccaaattaat aaggttatta tatctgactc aaaatctact
     8581 aattataaaa ataaaaattt tgacacctta attaaagagt atgaaaaaaa tataattttg
     8641 gatgcttatg aaaaatttgg tagttcttat aaagtagcca aacaccttga aatctcacag
     8701 tctaaagcca ataggttaat tagaaagtat actaatactt aattttaaat ttgataaata
     8761 aaaacttaac actatttaca tattgtaaat ttggccattt actgttatta tgaatatggt
     8821 tgaaattatt taaataaata ctgttgactt agtttcatat atttaccatt aaacttttat
     8881 cataaatatc atatcataat aatatattta ataagtctaa taaactatac gaggtgtaaa
     8941 tatgttaatg aatacccatt ttattatggc aaaatcaata cttgataata tagatgaaaa
     9001 taaaacattc tttataagcg aaaaaaattt tatatatggc aatataaagc ctgatgcttt
     9061 ttctaaatat aaattaaaaa aacattattt agatgagtca tttaatatga ttgtttctaa
     9121 aataaactat ctatgcagtc ttaatataga tagtctttct aaatactttt cagtaagtag
     9181 acttagtcag gaactaggag tcatatgtca ttttttatgt gattttttct gtgtagctca
     9241 cagtgaacgt tgggaattta agcatagctt aaataaacat gttacttatg aaagagaact
     9301 tggagcattt gcaaaagatg ttgatttatc taaaattaaa tgtggtacta taaatagtag
     9361 ttttgagaag ttttttacta aactttatag tgattacaaa aagaaaactg attatatgaa
     9421 tgatttaaaa ttttcagctt atacttgtaa tagtgtcata aattatatac ttgactctat
     9481 tttatctaat acaataaaat catatcatat agcaaattgc ggttaactta agaattttta
     9541 aatttatata aacttataat tgaatataat taaaatgact gctctataga atataatttt
     9601 atacttctct aggacagtca ttttttatac taactattta tctccattat aacatctatg
     9661 gcacttttta cactatctac tacataattt gcactttgct ttacatcttc ccttgcctta
     9721 gacattgcaa aactatattt aaatccttct aacatgcaaa tatcatttgg agaatcccca
     9781 atagtagcaa cttcatctgg acttacattt aacgttttta ttacatgctc tatacttcct
     9841 ctttttgaca cacttttagg aactatattt aaagtatatg ttccagaaaa atatatttct
     9901 aatacttcac taaactcact ttttagataa agttctattc tggataactc ttccaaattt
     9961 tcagaacata cgcctatatt tcctataaga ttatcaaaac tacttctttt ttcaaaatct
    10021 gggtcaacta taaagtcact agaataatgt ctaaaagcag tggtatcact acccacaatt
    10081 acatcaccct tatatttaaa ataaagttgt gcatcacttg attcaaatct atcaatgact
    10141 ttttttacta aattatcatc tatcgttctt ttaaaaatta cattataatc tttatcatat
    10201 ataaatgaac cattttcaca tacataatat ccattcatat ctaaactatc tttgataata
    10261 ttatgtgctt gtttaaaaat tctacctgta gcaacattaa actgtactcc ctttttttct
    10321 atttcttcaa ttgctactat atcttctttt gttatggttc cattttcata tagagttcca
    10381 tctaaatcac aaaatatatg ttttatcata aattaaaagc ctccaaaaac aattttatat
    10441 tatagtaaca cttcaaatat atccaatatc ataacaactt aaagcttttc taattgtatt
    10501 ctcaattcat taagtaaatc tctagtagct cttaatctta tctttcttct atccccatta
    10561 aatatatttt tctttactat tgtttttcca tttatatata ttgcagtata aactagtccc
    10621 acaggcttct ctttggttcc tccaccaggt ccagctactc cagttgtgga tatacctata
    10681 ttagctttaa gtcctttagc aacgccctca gccatttcta tagctgtttg ctcacttact
    10741 gctccaaact cttccaaagt ttctcttttt actcctaatc ttttcatttt agaatcatta
    10801 gaatatgtta cacatccttc aagaaataca gaagatattc ctggatagtt tataagagat
    10861 gaagaaacca aacctccagt acaagattca gccactgcta ttgttagatt tttttctaca
    10921 agtaacttag aaacagcatc ttctaaagca gtctcccctt cagatatgga aatatctcct
    10981 tcagtatata catatttacc aactctactt tttatttttt cttctactgg ttttattaat
    11041 ttaaaagctt ctctttcatt ttcagccttt gcagttattc ttattgtaac ttctaattct
    11101 tttgcatata aagcaactgt tggattagtc tgctcattaa ttatatcgag aatttctaat
    11161 tctagatttg actcacctat tccatataat cttaaagtct tagatactag catttcattt
    11221 gtgaattgtt gtaagtaagg tttgacactc tcattaaaca tagctttcat ttctcttgga
    11281 ggtccaggca atactattat aaatttacca tcttttttta aaattgcccc tggagctgta
    11341 ccattattat tttttagtac tatagcatct tttggaaagt acacttgttt catattattt
    11401 tcggccattt tttttcctgt tttaacaaag aaagattcca aaacctctag tgaaggttta
    11461 tgtagttcta atttctgacc aaagtattct gcagcagttt cttttgtcat atcatcacca
    11521 gttggcccaa gaccacctgt agtaattaca aaatcacttc tctttaagct ttcatcaaaa
    11581 cattctaaaa gtctttgttt gttatctcca acagtactct gatggtatac ttctattccc
    11641 aatgatgcaa gttctttagc taaaaattga gaatttgtat ttactatatc tcccaataat
    11701 atttctgttc ctacacttat tatttcagct ttcaaaattt atcactcctt gatttatagc
    11761 cattaagact agtcttaagt tcataacaat aattttagca aaaataataa cataaataaa
    11821 gttaaaaaat catttaaaca tatctttaaa ctagattatt attctaaaac cacacataat
    11881 ataaacttcg tatatagtgg tactttcatc ctcaactgtt aattcatata attaagtatg
    11941 taaataaaaa gtttacttta aaataataaa aattaaaata ggagtgtata atatgaaaat
    12001 agcagttata atgggtggaa tatcatcaga gagagaagtt tctttaaatt caggaaaaga
    12061 gatatataat aaccttgata aaaataaata tgaagttgta aaagtaataa tagatgataa
    12121 aaaagatata tttacaaaga taccagaaga tatagatttt gcaatacttg ctttacatgg
    12181 aaaatttggt gaagatggat gtattcaatc aatacttgag actatggata taccatactc
    12241 tggatgtgga cctctttgca gtgggatgtg tatggataaa aatataacta aaaaaatgct
    12301 tagagattca aacctaccta ctgctccatg ggttttagta aaatcagttg atgaaatcga
    12361 ctatgatgaa atagataata taggttatcc agtatttata aaaccaaact caggaggttc
    12421 aagtgtagcc acattcttta ttcattctaa ggatgaagtt gaagaagcag ttagaaaagg
    12481 tctggaagtt gacgaatttg taatgattga aaaatatata ccaggtggag aatatacttc
    12541 attcatatta aatggagaag tgttccctac aatatctata aaatctgatt caggattctt
    12601 tgattatgaa gctaaatact cagttgaaaa aggtgcaaag gaagaagttg tttacttaga
    12661 tgaagaactt caaaaaagag taaacgaaat ttctgagact tgttggaaaa tttttaattg
    12721 caaagcatat gtaagagttg atatgattat aagtgaaggt attccatatg ttcttgaact
    12781 aaatactctt cctggaatga ctcaaactag tttaattcct agaagtgctg ctgctagagg
    12841 tattaagtac agtgaactat tagataaact tatagaatac tctttaaatt agatgaaata
    12901 atttgaggtg gtaatttgat attttcaaat ctaaaactaa atgataatga accaatttat
    12961 atacaactta aaaattatat aagtgatatg atatcaaaag gactaattcc agacaatagt
    13021 aagctccctt ctacaaggga gcttagtcag cttttacaag ttagccgaaa ttctgtagtt
    13081 ttaacttatg aagaattaaa atcagaagga ttaatctact ctatttcagg aaaaggtaca
    13141 tttgttaaat caaagaataa gtcttcaaat actacatggt cattaaactg ggattgttta
    13201 gagaatacat actctaaaaa agccaatgaa ttggacatta taaaaagtga aattccttgg
    13261 agctctgacc tgatttcctt taaaagtatt tctccagatg gagatttatt cgacatggaa
    13321 gaattaaaaa aatccttttt aaatagaatt tcactagaag gacataaatt actcaactat
    13381 ggatatgcac aaggttataa gccccttatt gactatttac tagaatatat gacaaacaag
    13441 ggtgctgata taagtaataa agatatacta ataacaaatg gattcacaga aggctttgat
    13501 attattatgt catcttttac ccaagaaaaa gactacataa tatgtgaaaa tcctactcat
    13561 aatactgcta taaaaattat gaaatctcat aacctcaata tcttaggtgt agatataaat
    13621 gaagatggtt tggacttcaa tatgctagaa gacaatttag ttaagtataa aaataaaata
    13681 aagtttgcat acattacacc ctcttatcac aatcctacag gaattgtaat gactcctgaa
    13741 aataggtaca aattctataa tcttatgaaa aaatacaata tagcaattat agaagatgga
    13801 tttaatgaag aattactcta caatagttct catatatttc ctatctgttc cctagataat
    13861 atgaataatg gggtcgtata tattggtagt ttttcaaaaa ttttatttcc tggaatgaga
    13921 attggatgga tatttgcaga taaaaaagta atcaataaac tagaaagtgt aaaaaggtgc
    13981 agaaacatac atgtatcctt tttagaccaa ggaattttat ttgattattt aagtaatgga
    14041 ggttttgaaa aatacattaa aaaaatacgt aaattctatg gggacaaatt taactttgca
    14101 tataaatgta taaaaaaata tataaaaaca gaatatatct tgggtgatgg aggtttgcat
    14161 atatttataa aactaaaaaa tatagatact agagttttat tagaaaaatg ttatgaaaaa
    14221 aatgttatat ttatgcctgg agatttattt tttacagata actctggttt tgatacttta
    14281 agactcggat tttctaggtt gtcttttgaa gacattgaaa ttggaataaa aataattgga
    14341 gaaactataa atgaaatgtt ctaggttaac taaatattgt tagcctagtt tatttttatg
    14401 aggtataata tatatagttt atatgaatct aatactgggg gataatatga gtaaaaagtc
    14461 aaaacgaaga aaaattaata aattaaaatt aacgattgta ataactgtat tatttcttgc
    14521 tttattatct atatatgaga tactgttctc taatcccaac aaaaaaggta atataaagag
    14581 tcaagaaact atgcagaata aaaataatac aacagatgat tctaaaaata atcaaagctc
    14641 taccaaaaag caggaagata ataatatcaa gcctactttt tcatattctt caataccaga
    14701 taatataaaa aataaaatga ttggtaaatc tatgcctact gatgaaccta taagctttga
    14761 tagtctatct tatttaaaac ttacatatta tggatttgat gaaaaaactc atcaaggaga
    14821 aatgatagta aacagtgaac tagctccaga agtagtggat atttttaagg aattatacga
    14881 aaaaaaatat ccaattgaaa aaattaaatt aatagatgac tatgatgctg tagatgaaaa
    14941 atctatgtct gataataata cctcatcatt ttgttataga actatagctg gtacaaatgt
    15001 agtttcaaat catggtaaag gacgtgctat agatataaac ccattacaga atcctcaagt
    15061 ttctggaaat gatgttacac ctaaaataag tacagtttat gctgataggt ctagcacaaa
    15121 atttggaatg ataaaaaaag gtgatgattg ttataatgct tttgtaagca gaggttggag
    15181 ttggggtggc tattggaaaa acccagatta tcaacatttt gaaaaataat agattagaaa
    15241 taaaggtgaa ttatatgaat ttaatgacat tggaaaatat aagtaaaagc tattctgaaa
    15301 aaaaattact agaaaatata tctcttggaa taaatgaggg ggaaaaaata ggacttattg
    15361 gtgtaaatgg tactggtaaa tctaccttac tcaaaattat tgcaggagtt gaagaaagtg
    15421 agactggtaa tataacaaaa actaatggta ttaggataga atatctacct caaaatccaa
    15481 cttacaatga aaattcaact gtacttgaac aagtatttaa tggaacttct gaagaattaa
    15541 agctacttgg tgaatatcaa gaaatcctag aaaaacttaa ttcaaacttt acagaggaat
    15601 taaataaaaa attattatca gtacacgaaa aaatagatgc cttaaatctt tgggatttag
    15661 aaagtgaagc taagtcagta cttacaaagc ttggtataaa tgactttaat caaaaaatag
    15721 cagaactttc tggtgggcaa agaaagagag tctctctggc atcagccctt attacacctt
    15781 gtgagttttt gatactagat gagcctacaa accatctaga caatgatact atagattggc
    15841 ttgaggaata tcttaattca agaaaaggct cattgcttat gataactcat gatagatact
    15901 ttttagaccg tgttacaaat agaattattg aactggacaa gggtagatta ttcagttatg
    15961 atgggaatta ttcaatattt ttggaaaaga aaatggaaag actttcgcta gaagcaagta
    16021 ttgaagataa aagacaaaat ttaataagaa cagagcttgc gtgggtaaaa cgaggagctc
    16081 aggctcgtag cactaaacaa aaagctcgtt tacaaagatt tgatgaactt atgaatagag
    16141 attcttttaa acctgatgaa aatctcgata tatctgttgc atcaagtaga cttggaaata
    16201 aaataataga aatacacaat atatctaaat catttgaaga aaatactgtt atagataatt
    16261 tagaatacac tttaactcgt acagatagaa ttggtataat aggcaaaaat ggtatgggta
    16321 aatctaccct tataaatata cttaatggag aaatagagcc tgatagtgga tatatttcaa
    16381 ttggagaaac agttaaaata ggttgttttt ctcaagacga ctctcatatg aatattgata
    16441 tgagggctat agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta
    16501 ggattacagc ttctcaaatg tgtgaaagat tcctttttaa tggtactatg caatatactt
    16561 tgataggtaa attatctggt ggtgaaagaa gaagactaca tcttcttaaa atccttatga
    16621 cagctcctaa tgtgcttctt ttagatgaac ctacaaatga tttagatatt gaaacgctta
    16681 aaatattaga agaataccta gacgaattta aaggtgtagt agttacagta tcacatgata
    16741 gatatttctt agatagaatt tgtaataaaa tatttgcata tgaaggtaat ggaaaaattc
    16801 atatctatac aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat
    16861 ttgaagaatc taaaagggaa tcagttaaaa aagatgactc tactacccta aaaaaagaaa
    16921 aacctaaaaa tgataaaaag cttaagttct catataatga acaaagggag tttgaaaata
    16981 ttgactctga tatagaaaaa ctcgaatcta aaattgcaac acttgatgaa agtactgcaa
    17041 aattttcaac tgattttact aaacttcaag aaattttaga tgagaaatct aaactagaaa
    17101 aggaacttga atacaaatat gaaagatggg aatatttaaa taatctagct gatgaaatag
    17161 ctaataataa gtgatattaa aataatctta agtttaataa gttttgcttt aaatctatac
    17221 tttataaata ttttaaaggt agtgtctcaa aatgaacttt ttagttttat gagacactct
    17281 tttttatgag aaatcaaata tgttttcatc atttcaacaa tacaaaagag gcttatctct
    17341 attttgagac agcctctttc ataattacct tctaatttat atgctttaat atatctataa
    17401 taattcttat tatatctata tatccagtct ttcaatatag tgacaattaa cttcaatatg
    17461 atttaaaaat actaacaacc tttcataaag tggatgagat tcttctccat atttagcttc
    17521 taggttcttg cctatttctc taacagtcct ttgaccatca atttgtaaaa aaacaaaact
    17581 ggaatattca tctagttcta tttttttgta tttaggtatt ctagctttta actttctaaa
    17641 aaacttttga attttatggt cctgtttttc aagtatagta acaacactat tttcagctac
    17701 ttcatattca agtccatcag taattttaaa tacaatatct aaaacatcat tactcttgct
    17761 cattattttt tactttagat aaaacaatag gtaatgttgt aagcacaact aaaactacta
    17821 ataaagcaat tgccattgaa tttgttgcag caaatccact tggtaccttt ggagttacaa
    17881 ctccagttac ttgtaatatt atacctatta aaccaatgat agaaccacca gcaacaagac
    17941 cagaagataa acttatacca ttagaaactt taacttcttt ttctttttca gttttagata
    18001 ctaattcaac aaataaacga attaatgctc caactaatat tatagaagtt gttgcaattg
    18061 gtaaatagaa tcctatagct atagtcatta ttggtaaatt taatgcatat agaactattg
    18121 ccataaatac tccaacaata atcataaccc aaggtaaact acctgacata ataccagaag
    18181 ttaatgttga cattaagttt gcttgtggta atgcaaattg tacattatca ccagttgctc
    18241 taagttggtc agaaagtaat aaaattactc ctacaacaac tataacacca actatacttg
    18301 caattgtaaa gtaacgttgc atttcgtttt tgctaccacc aataatataa gttacttttt
    18361 gagattgagt ataacctcca gctatagcaa tagcaacaac tataaatgaa ccaaataaaa
    18421 gtaatgattt attagcttct aaatcagtcc atcccattat aacaaacact aatgttacaa
    18481 taactagaga agctatagtc ataccagaaa ctggaagatt tgatgttcca attgttcctg
    18541 ttaaacgacc agctactatg acaaataata aagataataa aagagatatg atagctccaa
    18601 taattgccat aacaatgtta ccagaaatta agaatgctgc taaaaatcca ataactactc
    18661 cacctaataa aagtatcatt tgtatagatg aaccttcttc tccttctcca gcacttgatt
    18721 tagctttaag tgtttctttt atagaagcga ttatagttgg tataagtttt attgcaccaa
    18781 taatacctcc acaaagcatc ataccagcac ctatatattt tacataacta ctagatattg
    18841 ctccgacatc catttggtta agaggcattg caggattatt ccataccata gctccatctt
    18901 tagccatatc tgtaaagtat cctattaaag gtgctatacc aaagtttgat aaaatacttc
    18961 ctgcaaacat tgttaaagaa acttcaagcc ctactataaa tccaattcct aaaagtagag
    19021 gatttacttc aatatcaaat ttccatttgt agaatttgct tcctacaaga ctcattacat
    19081 tgtttgctac atttaagaat gaaccagtta atacagtaat aaatccactt attacaaatc
    19141 caattcccat gtacttaatt gaatctccac cagcctctga agcaacaagt gtctcagata
    19201 tagccattga ttctggatac ataagtttac catgttcttc aactattaga tagttgtgaa
    19261 ctatagcagc aattcctata ccaaataaaa ctccaccaac tccaacagct aggccttcaa
    19321 agaatgtaat ttggcttcca attaatataa ctgctggtaa tacgaaaata aatccacttg
    19381 caacagattc tccaccactt gacatccctt ggattaagtt tttaccaaga ataccctttg
    19441 aacgagcaaa tgctccaaca aaagcagaac ctattatagc tcctggaata cctgcagcaa
    19501 ctgtaagacc ggctttcatt ccagagtaag ttgtagatgc ggcaaaaatg gcagctaaaa
    19561 taataccgat aatcagtaca gctacatttc caccggttct ggacttatct gtgatatatg
    19621 gaacgtaatc ttttccacta acctcaccat aagcaccctt aggtaatttt ttgttcataa
    19681 aattttcacc ccttttcaat ttgtatatat tataacatat atacatataa aacttaactt
    19741 tttattattt tacaaatagc gagtcaaaac tactaaaatt taaccctata ttattaattt
    19801 ctacttttag atatttttat gctaattttc tttttatttt tcaattctac taaaaactat
    19861 taagatataa aataaaaaaa atttttaatg taaaattaat tttgtaaaaa aaatgcttta
    19921 aaacacggaa aagtataaaa aatacaataa tcattcttat aaataaattt tcaccatagt
    19981 aaatatatac agaaaattct tttttttaca tcaattgatt tcctcagaaa aataggtgtc
    20041 ataaagaaat gtatggaatc actttacaac acctgtcata ataaaaaatc actctggtag
    20101 tgctttttta tttacttttc ctattggtgt aactggtatt ttatctatcc tcattattat
    20161 tgttggaatc atatatggag gtagatgctc atttaaatat tctctaatgg ttctttcatt
    20221 tatcttacca ctatcttcct catcaattac ataatatgca catatataca tatccccgtc
    20281 atcatttgtt ctcgatatac aaacaacact tttaatttct gtaatattat ttattatatt
    20341 ttcaatctca tatagctcta tcctaaactc atcaattttt acttgattgt ccatccttcc
    20401 aatataatct aaatttccat catctaacca tcttactaaa tcaccagttc tatacattaa
    20461 cttattatat tcacaagttt tgtaaggatt ctctacaaat acttgctctg ttagcccttt
    20521 tttatttaaa tatcctcttg atatttgaat gcctgataga catagctctc ctggtgctcc
    20581 aattggacat agtttgttct ttttatctaa tacatatgca taagtattaa atataggttt
    20641 tcctattggt atttttccat attctctgtc cacatgaaaa gaagtcgata caacagtaaa
    20701 ttcggttggt ccatactcat tatatagttc ataattacgc tttctatatc cttttaatct
    20761 ttctcctcca gtctgtacaa ctctaagtga attattattg caattttgta taaataacct
    20821 tgcaaatgct gttggcaatg ctagtatagt tatgtcattt tcctcacagt attcatttac
    20881 tgtatattca tcaaatttag cttcttcagg cataatatct attgatgcac ctacaagcaa
    20941 ataagggaat agttgtttca ttacagcatc aaaactgaaa cttaagtata taccacatat
    21001 atcattttct gtagcgctta tagattttac gctataataa cacatattta ctaaactctt
    21061 atgttctata gctatggtct ttggcctacc agttgaccct gaagtagata tcgtatatgc
    21121 acaatttgat gatgttattt tttcagttaa ttcatatcca agcttaaaca tttcttcatc
    21181 atttatatcg agtactatgg tattctcatt tacaacctta cttttgagtt catctgtagt
    21241 tatcactact tttgcattac tatcttcaag aaggtaattc aatctttctt ctggattact
    21301 cgtgtctatt ggaaaaaatg ctccccctga tttcataatc cctattgctg taataattat
    21361 gttaatatct cttggtaaca ttacagctac tatgtcttca ctttccactc ctatatgatt
    21421 taaataacct gcaagtttat cagttacctc atccaattct ttatatgtta actttctatt
    21481 ttcatcccta gcagctatat tactaggtgt taccttaact tgctccctaa ataaatcaac
    21541 ataactttta tcatagggta cttcatattg attattatta aagtcttcca ataatacttt
    21601 ttcttcttcc ttacatacaa attctaaatc ttctattaaa atatcattat cttctattat
    21661 ttgctctatt atataatcaa attgatttaa aattcttcta atagtttctc ttttatatat
    21721 agatggttta tactttattt ttattttaag tgccctctta gtatatacta ttacagataa
    21781 atcatatcct tttttttcta tgtcataatc atatgataaa tcaaaacttt tagctataaa
    21841 tattgtgttt ataagcttag tatttagatg atttttattc tctatatctg ataacaaata
    21901 gtatccatac tctaaagacc tgctccactg cctttttagc ttccttacaa catctataaa
    21961 tttttcactt tgtctaactc ttactcttat tggaattata ttcattgttt taccaaaaac
    22021 tacatcatct tcatatatat acttttgaag tataaggcca tatatagttt ctatgataga
    22081 gtctatgcta atattgttca ctttagaata ttgaattaac ctttcatgtt tctcaatatt
    22141 catcttaaaa acctcatcta ctacttcatc agaacaatct ttataaaaaa aatcatttgg
    22201 aatttttgct atattcgtat aatcatctaa caaatccatc caataattca tagctcgttc
    22261 taaattaact tcatacatat caaaattccc ccttactact aagtctatag aaaaaattct
    22321 tttggaattt tattttcaca atatataaat atattaataa tacatatgca agctctaaaa
    22381 acatgagctt gcataaaatt acacttatta tgactactaa attcgtaata tttacaatgt
    22441 ttaaaatata ttat
//
LOCUS       DAEPOG010000050        22380 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEPOG010000050 DAEPOG010000000
VERSION     DAEPOG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.050"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            202..1164
                     /locus_tag="KR432_001604"
     CDS             202..1164
                     /locus_tag="KR432_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0640145.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMYFMLILISSLCVIIYSYKITSLYIFKNFKIGILLVFYFLFSQI
                     IFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGI
                     VMVGTCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIM
                     HVLINSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            1544..3070
                     /locus_tag="KR432_001605"
     CDS             1544..3070
                     /locus_tag="KR432_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0640146.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            3098..3649
                     /locus_tag="KR432_001606"
     CDS             3098..3649
                     /locus_tag="KR432_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640147.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPYYSKKI"
     gene            4178..5221
                     /locus_tag="KR432_001607"
     CDS             4178..5221
                     /locus_tag="KR432_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640148.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            5332..6474
                     /locus_tag="KR432_001608"
     CDS             5332..6474
                     /locus_tag="KR432_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0640149.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            6928..8319
                     /locus_tag="KR432_001609"
     CDS             6928..8319
                     /locus_tag="KR432_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0640150.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            8572..10365
                     /locus_tag="KR432_001610"
     CDS             8572..10365
                     /locus_tag="KR432_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0640151.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(10446..11828)
                     /locus_tag="KR432_001611"
     CDS             complement(10446..11828)
                     /locus_tag="KR432_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0640152.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            12086..12433
                     /locus_tag="KR432_001612"
     CDS             12086..12433
                     /locus_tag="KR432_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0640153.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            12451..13485
                     /locus_tag="KR432_001613"
     CDS             12451..13485
                     /locus_tag="KR432_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640154.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(13534..14583)
                     /locus_tag="KR432_001614"
     CDS             complement(13534..14583)
                     /locus_tag="KR432_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0640155.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            14858..16393
                     /locus_tag="KR432_001615"
     CDS             14858..16393
                     /locus_tag="KR432_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640156.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(16579..17265)
                     /locus_tag="KR432_001616"
     CDS             complement(16579..17265)
                     /locus_tag="KR432_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH0640157.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            17390..18772
                     /locus_tag="KR432_001617"
     CDS             17390..18772
                     /locus_tag="KR432_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0640158.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            19174..20442
                     /gene="eam"
                     /locus_tag="KR432_001618"
     CDS             19174..20442
                     /gene="eam"
                     /locus_tag="KR432_001618"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBH0640159.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(20535..20891)
                     /locus_tag="KR432_001619"
     CDS             complement(20535..20891)
                     /locus_tag="KR432_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640160.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(21042..22136)
                     /locus_tag="KR432_001620"
     CDS             complement(21042..22136)
                     /locus_tag="KR432_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0640161.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         8863 a         2476 c         3609 g         7432 t
ORIGIN      
        1 taaacagaag aataaatttc taacgaaatt ggatatttct tctgtaaata catattcaaa
       61 aaaagaatta taagagttta gtcagcagtc gtgtaagtaa taaggacttt ataaatatat
      121 agaaatataa tgatatgagt ataagtagaa aaattttatg atttttacaa ttatttttta
      181 taaaaaggga ggttatgttt gttgaattta caatcaaaat gggtaaactt tttaaagcca
      241 acatatgtca gtgatgaaat aaaaagaaaa aaactacaag ttataatatt tttttctata
      301 acttatggtt tatcttattc actaggttta ttagcaatat gttttaatca tttaatagat
      361 tcagaaattt tagctcaatt tatgatgata ttgccattat cttcagtttc tattgcaaaa
      421 ttttacacag aaggaagaaa taatgataaa tataatttct attcatcagt aatattattt
      481 tttattattt acttttttat atttattatt gaatcattaa agttaataag tattaatcaa
      541 tttcaattaa tgtattttat gttaatttta ataagtagtt tatgtgttat tatttattct
      601 tataaaataa caagtttata tatttttaaa aattttaaga taggtatttt gctagttttt
      661 tactttttat tttctcaaat tatttttgga ttaataataa gtgataatca atttgattat
      721 aagaatttct tgaattatat tcttcttcct ataatatcat tagtatatat ttatcctttt
      781 ttatgtgaag agtatggttg gagatctttt ttacaaagta ttttatttga tagatttggc
      841 aaaaaaatag gtattgtaat ggtaggcact tgttggagtt tatggcactt acctttacaa
      901 tttacactat atagtcctca aactccaata attggagcta tagcccattt gatatatggt
      961 attggacttt ctatattttt aggttatgtt tatatgaaaa ctaagaacat atggttttgc
     1021 tctataatgc atgttctaat taatagctta ggattaattt tcaatgattc tgaaataatt
     1081 attaattacc atagtattat acaaagattg atatttatac tattatttta tactcctttt
     1141 ctatttacaa aagaatataa gtaagaatta caattaattt atataaaata aatttttaag
     1201 taaatatttt tatagagaga ttgttatatt acatatcaga aaaatattaa acgaactaaa
     1261 tcaataaaaa agttggtggc taacaattta taattgttag cacaatataa tcttatgtat
     1321 tgaaatctag aatatcctca tttttactta gcataaatac tattgattaa gaaaataata
     1381 tagaaattat aaatatatat aagtttagtc tggtgtataa aacaagaaaa ctaagtgatt
     1441 tttcatttag ctttttatta agcttatttt ttatactatt tataataaat aaactccatg
     1501 atgggaaaac tattactatt taatatgtaa aggagtattt taaatgaatg aatcatattg
     1561 gtttttaaac tcaagtccaa aagaatataa caaattaggt gaaaatataa aaacagattg
     1621 tcttatagta ggtggtggaa taactggatt gacaactgca tatttattag caaaagaagg
     1681 taaaaaagta gtgcttgttg aggctgataa aataggatat ggaacttctg gaaggaatac
     1741 tggtaaggta acttgtcagc atgatatatt ttactctaag atagagaaaa aatatggatt
     1801 agataaagca aaatcatatt ataatgcaaa taatgaagct ttaaatttag ttgaacaaat
     1861 aatagaagaa aacaatataa aatgtgattt taaaagggaa acttcttttg tatttacaga
     1921 aaaagaggat actataaaga atattaaaga tgaatatcgt acatgtaaaa aaataggaat
     1981 aaattgtgag tatcatgaaa ctatagaaaa tattccatta gatataaagg gggctattag
     2041 ttttactaat caaggtcaat ttaacccaaa gaagtatatt gatggattgg caaaagcagc
     2101 agtaaattta gggctaaaaa tatatgaaaa tacaccagta gtagatcttg aaaaaggaaa
     2161 aatatgtagg gttaaaacaa gagaagataa tataatagaa gctgagaatg ttataatttc
     2221 atctcatagt ccatggtatg atggattgaa tttttatttt gcaaaagaat atgcagagcg
     2281 tgcttattta atggttgcag ttttggaaaa taaattagca gatggtatgt ttataagtat
     2341 agatgaccca tcaataactt ttagacaata taataatgga agtgaaaatt tactgatttt
     2401 tggaggagga gaccataaag ttggccaggg aggaactgag aaagaaatat ttgatgattt
     2461 agagcattat ggtaaagaag tctttaaggt aaaagatttt aaggggaaat ggtctgctca
     2521 agataatatg agttttgaca atgttcctta tattggttat atcaacaaaa gagaagacaa
     2581 tatttatgta gctacaggat tctctaaatg gggaattact aatggaactg ctgctggaat
     2641 aataataaaa gatttgataa taaataataa ttctgattat aaagatactt ttaatccatc
     2701 cagattaggt agctactttt caaaagattt tataaaggaa aatgcaaacg tagcaataaa
     2761 ttatgtaagt ggaaaattaa aaataggtag tggagatatg cctaaaaata atggagaagg
     2821 aaagattgtt aatattgatg gaaaaagata tggtgtatat aaggatgata atggagattt
     2881 ttatattgta gacacgacat gtactcattt aggatgtgaa cttaatttta acagtgaaga
     2941 aaaaacatgg gactgtccat gtcatggttc tagatttgac tataagggaa atatacttga
     3001 aggaccagct cttaagccat taaaacttta tggacatggt gataatgatg ttaatcctaa
     3061 gttattataa aattattttt aggaaggtaa agtgtatatg aataaacttt taaagccaat
     3121 aactatacta ttgtatatag tttatatttt atctgccgta ttttttgtag caaacaaaga
     3181 atatggaagt ctaggtctag tagcaattag tttagttggt actttcattt tgagttttat
     3241 aaataagcaa actgaaagat tattgagtaa agaattatat ataatgcttg ctttatttat
     3301 aatgttttca tcattgttag gtacttgttt taatttttat gatataaatt attatgatga
     3361 ttttttgcat ttatggtcag gtataatatc ttgtattgtg gctttttcta taattaaata
     3421 cttttataca caaagagaga taagtaaaat gagtaaagtg tttttagtta tatttttatt
     3481 tatgtttagt atgggagtag cgagtttatg ggaaataggt gaatttttaa tggatacttt
     3541 tgttgggact actacacagg ccggggggtt agaagatact atgattgata tggtagatgc
     3601 tttaataggc acaattataa ctattcctta ttatagtaaa aaaatctaat atatagtttt
     3661 aaatttttta tattaattat aatggattta attaatcatg ttgagtttca aggtctatat
     3721 gattaattaa atccattttt gttatttata attattattg aatgtatttt tacatataag
     3781 atataaggtg atagatagaa attgttggat tatgtaaata attaatatat aataccatta
     3841 tgggcaataa atacaaatca aatgataata tagtctattt atgtaaatgt tatattgtat
     3901 cctaagtata atagtaagtt ttttgatagt ggtgtagaaa gtaaaaatct actagcctaa
     3961 attgatgtat ggattagttt aggaacagta attgcatatc ttaagtttga tgtcatgttg
     4021 ggctactaga aataggtcat ctttttcact tgaatatctt ttctttaaac atgaggagta
     4081 tcatatcaaa aacatatgaa aattaagtga tttataagaa cagtaatatt ttatatgata
     4141 gtatagaata gtaataaagt attaggagga acattgagtg ttaaaaaagt ggtttggtat
     4201 tgtgaaaaaa agacaaaaaa gtgagtcagt aaaagaagaa ggtgaagtaa tattaaaaaa
     4261 cgaaaaaata ttatctgaag aaaagttgat agatgaagaa ggagttgtag ttaatattga
     4321 taatgaaata ttaactaaag tagaagtagt aaatgatgat aatgaaatag aagaaaaaat
     4381 aatagaagaa gattggttaa ttagtgaaaa taccataaaa ttagatgata aagaagcaat
     4441 tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa gtgaaaaaat
     4501 aggtttaaat aagtttgaag gacttgacca aaatgaaaat tataatctag aaaaaaatgt
     4561 tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt acataaaaga
     4621 aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta aagaagagtg
     4681 ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag gatacataag
     4741 agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt atttaagtga
     4801 agtattaaat atagatgaat actgcaaagg tgaatggagc tatctcgata atagcaaatc
     4861 tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc tttctaatga
     4921 aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga attatagatt
     4981 tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta taatcaaaag
     5041 gggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag aaatatttaa
     5101 gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta tacaaatgtt
     5161 aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga gaagtattta
     5221 atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata ttattttaat
     5281 gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt aatgtctgaa
     5341 gaaaaaaatc caaaagaaaa tacattagat gaacctaaaa attcagataa tgtaaattct
     5401 aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa aagggaaata
     5461 aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg agctatatgt
     5521 ttaggtggag gctatgtttt tggtaaagaa gctggaagaa aattgcctgc tacttcaaaa
     5581 cactataata gcagtgaggt agtagctact attggggata aaaaaataac aggtgaaatg
     5641 ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat gactgatgac
     5701 gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga tatattatcc
     5761 tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc tcagtataat
     5821 aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt tttaaaacag
     5881 tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt agcatcaaaa
     5941 tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta caataaaaac
     6001 aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt tgatgataaa
     6061 ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga agaaatactt
     6121 aaaaaagccc aagctgggga agattttgct actctagcta aaaaatattc tgaagatagt
     6181 agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt ggaatctttt
     6241 gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt agaaagtgat
     6301 tatggatatc atataattaa gaaaactgat gaaaaatatc aaccatttga agaggtaaaa
     6361 tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca aaatttaaaa
     6421 gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga atagataaag
     6481 tcaatttatt aaaaaaaaga aaaattaact gtatggataa aagctatttt tactctcaat
     6541 atagagagtc taaagtagct tttattattt tgtaataaaa tctatttata tatatgaagt
     6601 gataaataaa taacatgcaa aaaaatatta aaaagaaata tttttaattt gaattttttt
     6661 aatgaaaatt tacaaaaatg aaaaaataaa caaaaaagaa ttgaatattt aagttgttat
     6721 ttaaatatat atatattaag attttctact gaagatacta aaaaaataat atttttcata
     6781 attttaaaaa taataataaa aaaatgaaaa tacaggcgat aatctattga gtaaacatag
     6841 aaaagatgat attataagta tgtgctaaaa tggttaaagc aatattaata ttaaattcaa
     6901 ctataaaaca aaagggggcg tttttttatg tcaagtacag aaagcgtatc taaaaaaagt
     6961 ggtcatccga agagtttttg gcttatttgc atgacaattt tgtgggaaag atttagttat
     7021 tatggtttaa cagctttatt ggtattattt tttacagcaa gtgtagctca aggtggttta
     7081 ggtctttcta ctcaagaagc aacatccatg tttggcttat ttacaggtct tatatatctt
     7141 acaccattag tcggtggctg gttagcagat aaagttttag gtcaacaaaa atgtatttct
     7201 ataggtgcac ttttaatagc aataggagat ttcgtactat ttgcttcatc tacatctatt
     7261 gccatggttt gggtaggtct atcaataatt attttaggaa atggtttttt taaatcaagt
     7321 tgttcaaata tagttgggga tatatatcct aaaactgatg tgtcaagaaa agatgcagca
     7381 tacagtttat tctatatggc agtaaatgta ggagcattta tagctccaat aatatgtgga
     7441 ttgctttctg ataatgtatt tgcagtaaga ggagcaagtg gagatatagt atcttatgga
     7501 tataagatgg catttttaat ttgtggtata ggcatgatgt taggtatgat tatatttaca
     7561 cttcttgcac caaaattatt aaatgaagtc ggaaaatatc ctgtgactaa aggtaataaa
     7621 ggtgaaaaaa ttgagtatgg accacttact aagttggaga aaaataggat aatagcaatg
     7681 gttattttat ttttatttgt ggtcttattc tggacagcat actatcaaac acaaagttct
     7741 tttatgattt atgtaaggga taatgtaaat agaactctat ttggatttga aatgccagta
     7801 gcatggctta catcactaaa tgctatatta tgtgtggctt tatgcccagt attagctacc
     7861 ctatgggtta aattatctca taccaaaaga ggtgatttct ctattccagt taaaatggga
     7921 ttaggaatga taataatggg tgttgggttt atagttatgg tatttgctac attagctaat
     7981 ggtccagatg gaatggtaaa ggctagtata ttgtttatag tattgactta tatatttaca
     8041 acagtaggtg aattattctt atctcctata ggacttgcaa tgttcaataa attagctcca
     8101 gctaagtatg ctacattatc tatgggagcc tggtatctaa caagtttttt tgcaagttta
     8161 ttatcaggaa agattgctgg ttttacagcc acacttggtt ttttacagat atttggagga
     8221 atagcaacag ttttaataat atttggttta atattattgg gaataagaaa tgtattaaca
     8281 agattaatgg ctatggattt actaacaaaa gaaaaataat tcatgacgaa ttattgtaaa
     8341 tagatgaatt acttcaaata aaggaagtta catttaaata taaaattaaa tgtaatttcc
     8401 tctttgatta tgaaaaaaag ctttacacaa atagatatta taattaaaat tataattgtg
     8461 ataattagag tgtataagat ttatataaat tatacaaaaa atatttatgt aataaacttt
     8521 acatacttat atatttagtt attcaaacta ttatttcgag gaggtcttgt tgtgaatata
     8581 aaagatagat taagtggatt aagaaaattt atggaagaaa aaaatataga tgcctatatg
     8641 attccatctt ctgataatca ccaaagtgaa tatgtaggag attacttcaa atcaagagaa
     8701 tttatatcag ggtttaatgg ttcagcaggg acggttattg tgactaaaga cgaagctggg
     8761 ttatggaccg atggaagata ttttatacag gcagaatctc aattagaagg aagtacaata
     8821 aaattattca aaatgggaca agaaggttgc ccaactacag atgaatattt atataaaaat
     8881 attcctgaag gaggaacttt aggatttgat ggtagagtta tatctgctag agaaggagct
     8941 acattagctg aaaaattatc taaaaaaggt ataaagatag aatatcagta tgatttaata
     9001 gatggtatat ggccagatag acctgcttta tcagatagta aagcattttt attagatgtt
     9061 aaatattgtg gagaaagttt tagctcaaaa ttagctagat taagagaaaa aatgagtgaa
     9121 aaaggtactt ctactcatgt aataactact cttgatgata tagcatggtt attcaatata
     9181 agaggtggag atgttaaata taatccagtt gtactttctt atgcagtaat aactttaaaa
     9241 gaagtttatt tatttgttga tgaaagtaag ttaaatgaag aaatattaga tgaactagct
     9301 aaggaaaatg ttcaaataaa gccatataat gatgtgtatg aatttgtaaa aaatatagac
     9361 aaaactgaaa aagttttatt agatggaact aagcttagtt atacaattta caataatata
     9421 ccttgtgaag ttgagaaagt tgatgagttc aatccagtaa tgttttttaa agctcaaaaa
     9481 aatgaggtag aattagaaaa tataagaaat tcacatgtaa aagatggtgt ggctttcact
     9541 aaatttatgt actggttaaa gaaaaatgta ggtaagatgg aaatcactga aataagtgct
     9601 actcaaaaat tagaggactt aagaagagag caagaaggtt tctttgagcc aagtttcaat
     9661 acaattgctg catataaaga gcatgctgca atgatgcact atagtgcaac tccagagtca
     9721 aattataaat tagaggcaga aggattattt ttagtagatt caggtggaca atattatgat
     9781 ggaactactg atataactag gactacagtt ttaggaccta taagtgatga attaaaacta
     9841 cactttactt ctgtagcaag aggaatgata aatctttcta aggctaagtt cttgcatggt
     9901 tgtagaggat ataacttaga catactatct agaagttgca tgtggaatat gggaattgat
     9961 tatcaatgtg gaactggaca tggtatagga tttgtgctaa atgttcacga agctccaaat
    10021 ggatttagat ggagagtagt acctgaaaga tttgatagtg cagttcttga agaaggaatg
    10081 gtaactacta atgaaccagg tatatatata gaaggttctc atggtataag aacagagaat
    10141 gaaattgtag ttagaaaagc tgaaaagaat ttctatggtc aatttatgga atttgaagtt
    10201 gttacgttag ctcctattga cttagatggt atagtaccag aattaatgaa taaggatgaa
    10261 aaagattatt taaactggta tcataagtta gtttatgata aaatatcacc tttcttaact
    10321 gatgaagaaa gagaatggtt aaaagtttac actagagcta tatagtttgt actagataca
    10381 ataaaaatcg aggtattata atgcctcgat tttttattgt atattttatt aactttaaaa
    10441 aatttttaat ttgataattc tactaatttt ttattcatta taagtagtat aattccaaat
    10501 atcaataaaa atgatggtat cactatgaag acttgtaaca taccaagttg gtctataata
    10561 gcttgcgtat agccagataa tttatttgct gcaaatttgc ctaaaaacca aacacccatt
    10621 aggaatgaaa tatacttttc aggcgaaagt ttatttacca taccataccc gactggagaa
    10681 aagcaaattt ctcctatact ttggaatgtt aaaaatccaa ctacccaaaa tacacttgct
    10741 ttgtgaattg aattagggtc tgttcctgtt tgtataactg ctactatcat aaacaaaaat
    10801 gctatagcta atagtataaa acctaagctc attttttttg gcacacttaa atcgcctttt
    10861 ttagtttgag ataattttac ccatactaaa gcagagatag gtccaagaat aacacaaagg
    10921 agaccattgt atgaatctat ccatgaagta ggaatttgaa aattgccaac agtaagattt
    10981 atgtgttcct ttgtatagag tgcaatagac atagaggctt ggttatatgc tatccaaaaa
    11041 aatactgtga aaaaagctaa caccaatatt actattattc tttttttctc aatcaaagtt
    11101 aaaggtcttg aagcgagcat tttcttatat tctagtgatt ttcttataga ctctctcccg
    11161 ttactagggt cattattcaa aggtttataa aactttctgt ttccaaatat aaagaagaat
    11221 aaacctatta gaaccgtaat tgatgaaaat aaaaatacat atctatatcc atacatactt
    11281 atagataatc cgcttttcat agcaaaccat tcattagcaa ctaaaccgca tattaaaggg
    11341 ccaaataaag tacctagatt agtagccata tacgtaatag agaaagctcc atcttttctt
    11401 gggtcatcat tggtatataa gtttccaacc atagtatgta agtttccttt ataaaaacct
    11461 attcctaatc cagataaaaa tatcatgatg tttatatcca tcttactatt tgctatgtat
    11521 cctagaaaat aaccaattga aaccattagc attccaagta taatacagtt tttgggacct
    11581 aaaaatctat cactgaccca tccaccaata actggagaga aataagtcca tgctaaaaaa
    11641 gatgcaagca ttgatgcgga ttctgctggt gttaacccta aaccaccttt ggagacttgt
    11701 tcagctaaaa acaataataa taccaatttc ataccatagt atgcaagtcc tacaaaggta
    11761 aatattgaac agcagaaata taaatctcta ggttgtttaa gtttttcttg cgtttttaaa
    11821 actgacatat gtcgagaacc tccagataaa tagtttaaca aaattttacc atactatatg
    11881 agtatatgtt tttttcatat agacgtcaac ttgtaagata atttaattaa gtcgaaatat
    11941 atgtaaaaat agtatcattt ttgtgtattc agtaatattt ttttatgata atatatgtta
    12001 ttattatatt aaaatacaaa catatttatg gaaaggatat gccaatttag tagttagatt
    12061 gattgtttgg caggtgggat gagctatgat aagaaagaaa tctgaattac aagtagaagt
    12121 aaaagaaaat ttaaatggtg gtataggaaa ggttaagctt gaaaacataa tacaaaatga
    12181 agagcttaaa ggaaaaggaa gactatttaa aagagtagct ttaccagtag gaagttcaat
    12241 tggtgtgcat gaccatacaa cagattttga agtatattat atactaaagg gaaaaggtaa
    12301 agtatttgat aatggggaat ttgtagaagt aaatgagggt gatgtagtgt atacagctga
    12361 tggcgaaaaa cattctatag aaaatatagg agaagaagat cttgagtttg tagcactagt
    12421 tttatatatg taaaatatgg aggaggagtc atgacaggca agatagaaga aattaaggac
    12481 aagataaata gattttgcaa ctccaattta aatcaagaat acaaagatat aagctttaaa
    12541 attttgcaaa accttttaga taataataaa gagataattc attcttctag agctgatata
    12601 tggtcttcag ctatactaaa tatagtttta gaacaaaatc ttttatacaa taagaagcat
    12661 ccactacata taacaaaaaa agagttttca aaacaaatag gtgtttcttt aaatactata
    12721 aataataaat cgtctttaat aaaagatgtt tgtagtattg actttttaaa tcaagatact
    12781 atagctattt ctaatgtaga attttgggta aaagagacca attccaaatc aaagtctgta
    12841 gatttagatt tagaaaagaa aaaaatacta tataaaagat atattaagct atcacaagat
    12901 gcgaatagtg atattgaatc aattagatat atggaggaag ctgtaaatat aggtaaaagt
    12961 atgattacta aagaagataa agaacttgga ttttggaaag gtttatctac cagagcctat
    13021 atgatagcac ttgaatccct tgctagaaag ttagaaagta ttaataatct aaaggagtcc
    13081 aaaaaagtat tagaatattt aatagaaatt aatcctagtg atgagcaggg aataagatac
    13141 aaattattaa atgtattaat tagattaaat gatagaactt caataaataa attatttgaa
    13201 ctttataaag aagaaaaatc tgctacatgg atgtacagta aagctcttta ttactttaaa
    13261 aataaaagta tgtttttggc aaatgattct attaaggttg ctagaagtaa aaataagtac
    13321 gttggattat atttgttaga ttggcgaaat gcttttggta gagaatttgt tacagaggaa
    13381 gaaaaggcag aagctgtcta ttactatgat gaaaacatag tggtttggaa tgaagttaaa
    13441 ggttctatgg attggctatt aaaaaaaatg ttggaatttt cataaaaaaa actactcctt
    13501 acttttatat aagtaaaagg agtagttttt aatttataca tctagtttta aatcttcagg
    13561 ttttattaga cctttttttc tcataaattc tgaaatcaac aaagttacaa tagcaggaag
    13621 tataaaatta agtaaaagta taccaaccca catagatata cctccttttc cagcactttg
    13681 catagcagtt actgttccaa attgtccaac caatccacaa gtacccatac cagaaccaat
    13741 aggaatattc tccattttga atattgttgt agataatggt ccaagtaata ttgaagctac
    13801 agtaggtggt atccaaattt tccaattttt tattatatta ggtacttgca acatagatgt
    13861 cccaagacct tgtgccacaa ggccaccaac tctattttct ctaaagctca taacagcaaa
    13921 gcctaccatt tggcaacaac aacctacagt tgctgcacca ccagcaagac cacctaaact
    13981 taacatcata caaatagctg cactacttat aggtaatgta agagctattc caacaagtgc
    14041 agagactaaa gctcccatta ttataggttg catagttgta gcttgcatta ttatatttcc
    14101 aaatgaagtc ataaaagcag aaacagctgg accaataagt tcagctacaa gtactccaat
    14161 tagtattgtc acagcaggtg taaccaatat atctattttt gtttcttttg aaactaattt
    14221 tccaacctct acagcaaaaa gtgtagcgac aaatgctcca acaggtcctc ctaattcatt
    14281 accacaagct ccaacaagtg cacaagaaaa tagtacaagt ggaggtgctt gaagtccata
    14341 cgcaatagcc actgctatag ctggaccagt aacctgttta gcaagagggc ttataacttc
    14401 agtaaaaaga gaaatattga aattatctcc taatgtattg aaaattgttc caattaaaag
    14461 ggacgaaaaa agaccaagtg ccatataact tagtgcgtca ataagatacc tttgaacaga
    14521 aatttcaata ttttttcttt tagcaaatga tttgaaattt ctttttttat aattgatttc
    14581 caatttaaaa tcctcctcat ataatataag tataatttct ttttaattaa ttatataact
    14641 tttcagctga caagacaaat gttatttcac taaaattaga ctattgtaat agtaagaaat
    14701 taacattaat tttagtttta aattttaaat ttatggttta taattagtat agggatattc
    14761 tgaaatataa taattttcca gtttatccca atatttaact taggtagatt gcttacaaaa
    14821 caaatgatta tatattaggc taaaaggaag tggatagatg atatttgtaa aaaataagaa
    14881 atacagaaaa attgccataa ttatatctat tcctatattg cttgttgcgc agtactacct
    14941 ttttaaattt ggaatactta gaccaatggt aaagggagta gaaattgaaa ttgtagatgg
    15001 ggaatatgtt aaagatatag ataaatatgt agtaaaatta aatgaaacag ttactttgtc
    15061 tacaggtgaa tatattaagg ttccttcgta tgctaaaaat ccaaatattt ggtttaatgt
    15121 attggataac tcagggactt taaaaataga tggaaataaa atgacagcat taaaggttgg
    15181 gtattcttct gtgggtatca tgaaaaattc aagagtatta aaaaaggcta ctataaaagt
    15241 agtagaccca gaggtagaat cactagatat ggagattaat ggggatttaa aacatgttgg
    15301 tgatagtgct actatagaaa gtacaataga agttgattat gataagttta aaaaatcata
    15361 taagccagtt tataagagta gcaatgaaaa tattttaaaa atcaaaggaa ataaagtaag
    15421 tgcagttgga gttggtaaag ctacaatata tgctaagtgt ggaaataaag aaattgagag
    15481 aacatttagg attcaagcta gagttgctga aataaatatg gatgatataa aaatagaagt
    15541 tgaccaaaat gaaaaattga aaccagatat aataacttct ccacctggtt tagaaccacc
    15601 aaaaatatat tatgagttag tagattcaaa attacctgta gaaagagcaa taagtttaag
    15661 tagtaatgga aatgttgtag gtcttagaga aggtaaagat agggtgagaa taacttgtgg
    15721 agataaatct aaagtaataa cagtaacagt agtcaaagaa actataacga atgactatat
    15781 acaaaacttg gttggttcac ataaaatagt aaacaataaa gttattatta aacttgaatg
    15841 ggattatatg aaagatgtct ttgattatga tatatatctt aaagataagt tgtcaggaga
    15901 atcatttaag aaaattaaat ctataactat aaatgaaagt gatttaggta agtctaacaa
    15961 ggtatatgca actatagaag tagatatgaa gggtaatcaa aatatagatt ttgatattta
    16021 tgtcataggg aaaaaggatt cagatacaac taagccaagt aatgtaataa atattactaa
    16081 tggaaatgga aattcaaata atgataataa tattaaagat atgagggtag aaaatatatc
    16141 agctaatgtt gatagagata ataatgtcat tagagttaac tggagtagta tatctcataa
    16201 ggactgtact tacagtatat atgtaaaaaa taatactaat ggagatggag aatttaatct
    16261 atatcaaagt gatatacagg gaaatgagca tactataagt attcctgaag gagatttaaa
    16321 tctagatatt tatgttgttg caaattataa tggaaatagc tctcaaaata gtgatgttat
    16381 aaatgtaaga tagatttgta tacaaattga aagtgttgag tttatttaaa tattaaatta
    16441 agaaaaagga tagtataggg taattatact atcctttttt tgttacaata tataggttta
    16501 aaatctctag taatataaat tataaaaagt cttatttaca caaatctata gatatttttt
    16561 ttattaatca atattgtctt aaattgaact tagtgcggct tctattgcat gtattactgt
    16621 cgtatcaaat agtggaatag aagtgtcatt ttgtttaatt aaaagaccta tttctgtaca
    16681 tcctaatata acaccctctg caccttgttt gccaagctta tctataatag ataagaaagc
    16741 tttttttgaa gattcagata taattcctaa gcaaagttcg ttaaatattg tgtcatttac
    16801 tattatacgg tcttcttcat ttggaattaa gacttcaatt ccattattaa taattacatt
    16861 tttataaaaa tcttgttcca ttgtatattt agtacctaat aaggccactt tttttatttc
    16921 tttttccctt aatactgtag cagttacatc tgctatgtga agtaatggaa tatgtatact
    16981 ttcttgtatt ttatctgata ccttatgcat agtattagta cagataacaa taaaatctgc
    17041 ccctgcctct tgtaatttaa ttgcagcatc agctaaaatt ttggcgcttt tttcccaatt
    17101 tccactagat tggcattctt caatttcttg aaaatctaca ctgtaaagta tacatttaga
    17161 agagtgtagt ccacctaatc tttcttttat tactgtatta ataacttgat aatatgtaat
    17221 ggtactttcc caactcatac caccaattaa gcctatagtc ttcatattat acctctctta
    17281 tttatttatt ttgaaatagc tatttacaaa tacaagtata gcatctaaaa tagaagcata
    17341 caataataaa attgtatgga gtacaattat aattaagagg agtaattata tgccaataaa
    17401 ttcttttgaa aactatccta tgacatggaa accaaaatta gataaccgaa aaccaccaat
    17461 atataaaact ttagcaatgt tattagaaga agatattaaa agaggaaatt taaatcctgg
    17521 agataaactg cctccacaac gggaattagc agattttcta gatttaaact taagtacaat
    17581 aacacgagca tttaaacttt gcgaagaaaa gggactaata tgtgcaaaag tgggaaaggg
    17641 aacattcatt tcttctgatg taaatgtaag taatacatta ttatatcaaa cggaatcaaa
    17701 agacataatt gaattaggga cagttcatcc tccatatgag caaaatacat atattattga
    17761 ctttattaaa aatgtactaa aacaaccaga aatggatagg tttttacaat acatgtctcc
    17821 atctggcaca tatatgcaaa aaaagtcaat tgcaaaatgg atagaaagaa ataatgtata
    17881 caccagtgaa gaaaatatat tattatctac aggagggcaa aatgctatat gtgcaacttt
    17941 actaggatta tttaaagctg gtgatagaat tgctacagat tctttaagtt tttctggaat
    18001 taagtcaata gctaaaatga taggtattca attagtccca attcctcaag aaaataatga
    18061 aatatcgatt gaatatcttg aaagttattg taaaaatgaa aatataaaag gcatatatct
    18121 tataccagat tatcataatc caacgacaca tactatgtct gatttatctc gtaaaaagat
    18181 agccaagata gcaaagcaat ataatataat aattattgaa gatgctataa atagcatttt
    18241 tagagacggt atacaaactc ccatattttc tttagctagt gataatacta tatatatttt
    18301 tagtacatct aaatttttat gtgctggtct tagagtagca tttgtagttg ctccaaaacg
    18361 atatatagaa aatttagaaa atgcactata taatatgaat ctaatggttt ctccatttac
    18421 agcagaaata gtacatcgtt tattatattc tcctattata gataaaatta tagaagagaa
    18481 aagagatgca attatagaaa gaaatgaagt tgctgataaa gttttgtcag attataattt
    18541 gattggtgat aagaattgca gttttcgttg gttacttctt cctgatgaat tggatggaaa
    18601 atcatttgaa atttctgcaa aaaatctagg agttcaagta tattgtgctg aacgattttc
    18661 agtaggaaac tctacagttc caaaagcagt tcgtatttgt gtaacagctc caaaagatgt
    18721 agaagaatta gaaaaagggc ttaatataat aaagtcactt cttaagagtt aaatttattt
    18781 tactattatt taattaaata aataaaaagg cggagaaatt tattataata aacctccgct
    18841 tttattattt aatgaaatac tttacttttt attaaaaaat ttaattttaa taatcttgaa
    18901 aatataatcg ttttatggta aaataattct taaaaataga aaaaattaat catttgttaa
    18961 aaaaaatgca taatttattg taatttattg acgaaggttg aaaaatattc ataaatgtgc
    19021 gaaataacag aaaaaatgca aaaattaatt caatttcatt tgttataaaa tctatatggc
    19081 aaaattaaat ctgcctatca gaatgatata aacacatcag aaaggaggac gctatcaaat
    19141 aatacaagta gttaactttg atagcaaaaa attatgaatg aacaaactag aatatcctta
    19201 gagagagctg ctgaattaaa atcaaaaatt gatgattata ttcaggctag aaaaacgatt
    19261 aacagaggtc ttgaaaaaga agaagagata aataaacgaa aacagaaaat attaagtatc
    19321 ttaaatggaa ctgaagagga ttggaataac tacaaatggc aattatcaaa tagaataaca
    19381 gatgtagata ctttatcaaa aattataact ctaactaaaa aagaaaaaga atatataaaa
    19441 gaggttggta ctcaatttag atgggcaata tctccatatt atttgagtct tatagaccca
    19501 gaagatatat gtgacccaat aaaattactg tctataccaa cacatataga gttggaagat
    19561 gagcaagaag atttggaccc aatgggagaa gagtatacaa acccagcagg atgtataact
    19621 agaagatacc cagatagatt aataataaat gtaacaaatg agtgtgctat gtattgtaga
    19681 cactgtcaga gaagaagaaa tattggacaa caagattctc ataagtcaaa agctattatc
    19741 caagaatcta tagactatat cagagaaaat gaagaaataa gagatgtact agtaactggt
    19801 ggagatgctc ttacattaaa agatgattat ttagagtgga ttcttagcca acttaaagag
    19861 ataccacatg ttgattatgt tagattaggt actaggactc ttgttacaat gccacaaaga
    19921 attacagatg aattttgcaa tatgctaaaa aaatatcacc ctgtatatat aaatactcat
    19981 tttaatcatc caatggaaat aactaaggaa tctaaagaag cttgtgaaaa gttagcaaat
    20041 gcaggagttc cattaggaaa tcaggcagta ttattaaatg gaataaataa tgataaattt
    20101 gtaatgagat gtttaaatca agaattacta aaaataagag taaaacctta ttatatattc
    20161 caaagtaaac atgtaaaggg aacaaaacat ttcaatacat cagtagatga tggtcttgaa
    20221 atcatggagt atttaagagg atatacatca ggaatggcta taccaacata tatagtaaat
    20281 gcgccaaaag gaggaggaaa gactcctttg cttccgcaat accttgtatc aaaaggaaca
    20341 gattacgtta tgcttagaac atgggaagga aaagttataa aaatggaaga tgaacctgct
    20401 gtagatataa agaaacttat aaaagagcaa gcacaggatt aaaaaagtat ttaaagttta
    20461 taaaaagaaa caactttaaa ataaaaagaa aatggatgtc aaatttatat caatttgcat
    20521 ccatttttta ttagttattt attattttgt tgttgattaa ctaattcaag atatccactt
    20581 gggtggaagt aatatatctc agaagtattt tcatttacaa agtaaccaat atcaccatct
    20641 gcatctggta aaaatacata accattaaga tttttagctt ttaatgcttc aaaatcacta
    20701 gcatttcctt gatatatgta atcaacttca ttgttgtatt ttactaaaag ttctttagct
    20761 tgttcttgag tcaatttagt attttggctt acaggaacat tcttttgaga atctgcataa
    20821 gaagattcat ttactggtgc agttaataac aaacaagata aaactaataa agatataagt
    20881 gcttttttca tactaaaacc ctctttcatg tttaattata taagattcat tacttaataa
    20941 tattgtatgt ttcctataat taaatattac cattaaagag aataatatta cagttacaca
    21001 gctgtaactt ttgattacaa aatcgtaatt aaattgtatt tttattccat ttgtatttta
    21061 tcatgtctac gatttacaaa ctcacagaag tatatcttta taacgcccat cacaggagca
    21121 gatagaatca taccaggtat accaaagaat cctccaccaa cagtaactgc taggattgta
    21181 aagaatgggc taagacctag ttgtccacct acgatttttg gctctataaa tgtaacttca
    21241 acttgttgaa ttattattaa gtaaattaaa gaagttaaag caattgttgg attggagaat
    21301 aaattaatta ttacaactgg tgccatacct attactggac caaaatatgg aaccatattc
    21361 atgacaccca taagtgtacc aaataatagt gcatattcag attttaaaaa gtaaagacca
    21421 attccagaaa gtactccaac tataaaagaa tctaaaatct tacctgtaaa gtatttacca
    21481 atatttaaat ttaagctttg acatagttct attataaagt ctccatattt tttacctagg
    21541 gaaatataaa ctactttttt tgtaaacagt aagaattttt ctttttctaa taatatatag
    21601 aaacaaatta taaaacctaa aagaaattgt actataaatt ttccaactga aaatgtagtg
    21661 ctaaatatcc caccaagtga accaataagc aagttactaa atttaggcat tacactcata
    21721 attttgtcac catactcttt taatgtatta gggtcaactg attgtaaact cttacctaag
    21781 tcgaataaga actgctctgt tttatctatg tatgtaggaa tttgagcaac taaatcagct
    21841 aaactattta ctatggatgg aactgtaaat aatataaagg aaattagtat aactatgagt
    21901 acaccataag taagaagtaa cgaatatatt ctcttaaaat ttaatttact ttctaagaac
    21961 tttactagtg ggttgaatat atatgctaat acaaatgcta ttataaatgg tgttagtagt
    22021 gacattaata aacttatcac tccaaaaaaa tatttgtaat tatcaataac tttaatcaag
    22081 atatatgaaa caactacagt gagtacaata tttagataaa tttttttatc ttgcaaaata
    22141 acacaacctt tcttatattt attaaattta gatatactta aatctaagtt aaagttaata
    22201 tttatagctt attatacaat aaaaagcagt attaatatag tatgattaat atgaaattat
    22261 aaatacgtta aaactttatg cttatagcta aaataataaa aattatttat attatgttta
    22321 tatataatct aaaatgaaaa aaagtggtgc ctttgattaa actaagtttt tataatttaa
//
LOCUS       DAEPOG010000051        22233 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEPOG010000051 DAEPOG010000000
VERSION     DAEPOG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.051"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            493..1197
                     /locus_tag="KR432_001621"
     CDS             493..1197
                     /locus_tag="KR432_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640162.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            1199..2686
                     /locus_tag="KR432_001622"
     CDS             1199..2686
                     /locus_tag="KR432_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640163.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2891..4504
                     /locus_tag="KR432_001623"
     CDS             2891..4504
                     /locus_tag="KR432_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0640164.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGNKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(5194..6024)
                     /locus_tag="KR432_001624"
     CDS             complement(5194..6024)
                     /locus_tag="KR432_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0640165.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            6097..7824
                     /locus_tag="KR432_001625"
     CDS             6097..7824
                     /locus_tag="KR432_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640166.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7861..9723
                     /locus_tag="KR432_001626"
     CDS             7861..9723
                     /locus_tag="KR432_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640167.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            10056..10745
                     /locus_tag="KR432_001627"
     CDS             10056..10745
                     /locus_tag="KR432_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBH0640168.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10850..11536
                     /locus_tag="KR432_001628"
     CDS             10850..11536
                     /locus_tag="KR432_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBH0640169.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11553..13460
                     /locus_tag="KR432_001629"
     CDS             11553..13460
                     /locus_tag="KR432_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBH0640170.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13913..14602
                     /locus_tag="KR432_001630"
     CDS             13913..14602
                     /locus_tag="KR432_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640171.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            15038..15577
                     /locus_tag="KR432_001631"
     CDS             15038..15577
                     /locus_tag="KR432_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640172.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15596..16639
                     /locus_tag="KR432_001632"
     CDS             15596..16639
                     /locus_tag="KR432_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640173.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16641..17477
                     /locus_tag="KR432_001633"
     CDS             16641..17477
                     /locus_tag="KR432_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640174.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17582..18256
                     /locus_tag="KR432_001634"
     CDS             17582..18256
                     /locus_tag="KR432_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640175.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            18256..19308
                     /locus_tag="KR432_001635"
     CDS             18256..19308
                     /locus_tag="KR432_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0640176.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            19752..20600
                     /locus_tag="KR432_001636"
     CDS             19752..20600
                     /locus_tag="KR432_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0640177.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            20590..>22233
                     /locus_tag="KR432_001637"
     CDS             20590..>22233
                     /locus_tag="KR432_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0640178.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRID"
BASE COUNT         8729 a         2215 c         3816 g         7473 t
ORIGIN      
        1 ttagtttaag aaatccataa tattagtgaa gaatgaaata agtattatat atgaataaat
       61 atttttagaa ttaaggtgaa ctgtagtgct taatataact ataatattaa ttgtttgaat
      121 attcaataaa aagtttaaaa aatatcaatt ttgatataaa ctgaataaat acagttgaat
      181 attaaattat tattttatca aaaatatgca aagtactaca ccttcaaata tcacctaaag
      241 taattaaaag gcaattaaag tgaagtagtt caagtatttc taaaatgagt ttaataaatt
      301 ctataataat ttattttttg ccttaaaatt gccttaaaat ccaaacaatt ttaaaactaa
      361 tgtactataa aaagctggta taatttaaat atagtaaaaa ataagattta tcttatctat
      421 tgggatttaa ttatttaatc aatatattta taccatttaa tttaaataaa aagtctatta
      481 aggagtgagc ttatgagtaa gttaatttat atagcagatg atgaagataa tataagaaat
      541 ttggtaaaga cattcttaaa aaatgaaggt catgatgtta tggattttaa aacgggtgat
      601 gaacttttag aacaatttaa tataaaggag tgtgacttag taatactaga tataatgatg
      661 cctggctcaa gtggttttga agtatgtaca aaacttcgtg agaaaagtac agtcccaata
      721 atcatgctta ctgcaagaga tacagatata gattatatta cagggataac cttaggtagt
      781 gatgattatt ttacaaaacc atttagccca atgtctttgg ttatgagagt aaaatctata
      841 tttagaagga tagagtttga aaagaaacaa aattatgata aatatagtaa ctcgatagat
      901 atggaattaa aatttggtga tgtaataata aataaaaaga ataaaatcgt tacaagtaaa
      961 aatgtaaata tagaccttac accaaatgaa tataatctat tgacttattt gtttgaaaat
     1021 atagatagag cagtttccag agatgaatta ttaaacaaaa tatggggata cgatatagaa
     1081 gttgagacaa gggctgctga tgatacagtg aagagattac gtaaaaaaat tttagataca
     1141 aatattttaa tagaaactgt atgggggttt ggatttaggt tgaaggagaa aagttaagat
     1201 gaataatgga aatgacaaga atagaattgg aaagattaaa tccaaattta aattaaagtt
     1261 ctcaaagaaa aatataagca taaaaactag gattttgttg agcatacttt gtttgataat
     1321 tatagtattt atgataatat tagtatcttt taatgtattt cttgatacat atataaaaac
     1381 aactgcgaat gaagaattga cgaagtctac agaaatagta gaacatatgg atagtaattt
     1441 tagacctatt aagccaccac aagatgatgg aaagcttcct cccaaagtat tatctaattt
     1501 tatgagaaat gtacaagata aagttaggat ggcagaaaca caatcagatg ctgatgctat
     1561 ggtagttgat tctaagtata acctgatatt tccaacaaga gaggatgatt tcttaaaaaa
     1621 tgttgatgag atggagctga ttagggagtg tattcaaaat gaaaaattag atttaaattc
     1681 agatgaaaat acacggatat ctaccagaaa taaggattat tatatttcaa ctgtaaaaat
     1741 tactagtata tatggtgaac aagacaaata tcttatactt tttatagaca tatcaaaaac
     1801 tctaaatcta gcacaaaaga taaatattgt attaatatct gtaatgtgtt ttgctggaat
     1861 attagcaata tttacagctg tgattttatc tgagaaaatt gctaaaccga taaaagagtt
     1921 gtgtgagttt gcaaaaaaaa tgggtcaagg agatttcaaa agaacctact ttgacttttc
     1981 agataaagaa ttagtagagc tctcaactgt tatgaataag agtgctgagt atctggataa
     2041 gtatgataat gaacaaaaaa tattttttca aaatgcatct catgaattaa ggacaccact
     2101 catgtctatt aaaggttatg cagaagctat aaaatataat gttatagata gtaaacatgc
     2161 aagtgatata attcttgaag aaagtgatag gttaagtgat atggttgaag atttattata
     2221 catttctaaa atagataata taactaaaga ttttgaactt gtagaatgtg atttaagaga
     2281 agtattttca aattgtggag ctaggcaaaa tgtaagagct ataaataagg ggataaaatt
     2341 tatttatgaa tttgatgaag aaatggtttt atttgaatgt gatgaaaaaa acatttccaa
     2401 agcatttatg aatcttatag aaaatgcttt gagatacgct aagagtgaaa taaaaatagt
     2461 gtgtaagtat aatcaaaaaa atatagttgt gataatagaa gatgatggaa ttggaatcaa
     2521 gaaagaagat ttacctcatg tttttgaaag attctataag ggagtagggg gaaatcatgg
     2581 aattggatta tcaatagtta aatctattgt aaataagcat ggtgggcgta tatacgtaga
     2641 aaatggcaaa aaaggagcta agtttactat attatttaaa ttgtaaaaaa attagagaaa
     2701 atataaagat gtttgaagtt gttttttata tatatctgtt gtatactgtt gaaaaaagaa
     2761 caatattaag ctgacatata ttacaataat tgacatttaa agtaaatgct taaatataga
     2821 tacgaaagga aaagtaaaat ctgaagtgta tttaggttta agtaagcttc ttattttata
     2881 aaaaatatgt atgaaagaac agctgatttt taatattaca aatttagtaa cggttgtatt
     2941 tgaagctttt gtcatccata tgtttttatc agacttttta ggtcagaaag aagggtatat
     3001 aaaagttgta agatatgcta aactgggttt tataatatgt ttaggattct gtaatttaat
     3061 aactttaaac cctaaaatca caatgccttt aatttttatc cttatttttt caacatcatt
     3121 tttatataaa ggaaatttaa agacacgatt gtttactact gttttattaa gtatattctt
     3181 tatactgtca gaaattgtag ttacaagtat ttttgtttta tttgtaaaag aaggctttga
     3241 aataatgctt gaaaataata gtataagagt attggcgaca attttatcta aaattgtctt
     3301 tttgttgact tgtaaaatta tttgtctatt taaaaaagat gtacatttag atatgcccat
     3361 aaagtattgg ctccctttat ttttaatacc tatatttagt ttatttctgt ctgtttctat
     3421 ctttgatgta agtaaatttt ttagtcttga aagtttaaaa tttctaagtt taatatctag
     3481 tgttggcatt ttatatatta attttattgt attttattta tttaaattta ttatagataa
     3541 aactaaacta tctatgaaat atgaaatact tgaaaaagaa ataattcata aggaagaatt
     3601 aagacttagt aatgagtgtt ataaaattat tgttgaacag actgattctg tcgttttcga
     3661 atggaatatc aaagaaaata aatcatttgt atcgcaggct tggactgaaa aatttggata
     3721 taacaatgca tgtaagaata tttttaaaga aattaaagat aaagacttag ttcattctga
     3781 agataaagct atatttgaag gttttcttga aagtattaaa aaaaagaata tacacagtca
     3841 agcagtatat cgtttaaaaa aatctaatgg tgaatatatt tggtgtagaa catcaattac
     3901 aagcatttat aatgatgaaa atgaactttt aagagttgta ggagtaattg tagacgtaga
     3961 tagtgatata aaaaaatatg aagagttaag aactagagca gaaagtgact cattgaccaa
     4021 tatatacaat aaaggtacat ttgaaaaatt agtggaagaa actatagtta tgaatacagg
     4081 tgataaaaaa gatgcattat tcattattga tttagatgat tttaaagaaa taaatgataa
     4141 ttttggtcat ccatttggtg attttgtact aaaaacattt gctgataaga tacaagctag
     4201 ttttggtaat aaagacttag tcggaagaat tggtggagat gaatttgttg tatatatgca
     4261 agattatgtt acagaagtaa atttgcataa aaaagctaaa gaattaaata gagtactttc
     4321 ggataattac actgatttga gttttagttt tgattcttct gtcagtatag gtattgcaag
     4381 gtatcctcaa gatggaactt cattttttga attgtttaaa aatgcagata gagcacttta
     4441 tagcataaaa gcatcaggta aaaattcata ttgtttattt gaagaagaac tatatgtaca
     4501 gtgaatttga atttagattt tatgtgagag taagatttat atatacttgt ggctgtctga
     4561 tttataaaat atgttacttt gtatattttt atatgtttca gtaaggttaa ttaacataag
     4621 caaaggtgta agtaaattta tattagttta agtttactta cacctttata aaaataaatt
     4681 ttgtaaattt aaacagttgg ttttataagg atttttattt attttaatat cttagatatt
     4741 cctttttgta ttccttagag aaaataaata atgaaaggag tacagaaaaa attaaacatc
     4801 ctaatatacc acttgaatta agtcttaaag cattaaagtt taaaaaagca ttatttaaat
     4861 cggtatccaa aagaaaagga tgctttgatt tgctatatat ggaaaaatta aaaatatatt
     4921 cataatcaaa tttataaaat taatatcttt ccatcctaac attacattta cttagattaa
     4981 ttattattat aaaaataaaa atacaaataa tagattcatt tacattctta aatgattaaa
     5041 gctctttacc ctttaaacat gcataacata tcgttataat tccagtaaat aagactaaaa
     5101 tgcccattcc taaaattgca gtttcatcat ctattactcc aaatgctttt aaagtaactt
     5161 atttcgcttt gtataataat aatttgtctc tttttatatt ttaacttctg ctgagatttg
     5221 agttagatat tcgttagaat ctcgcacact tatatcatat agaagatatt cttcataaac
     5281 aaaatcccca atacgtaaat tattctgatt aaaaaaattt aataactttt cataagcctt
     5341 gtaaatagtt tcataactac ctttatgata agcagttaca taaagtccct ttggtttgat
     5401 tgatacagga tatttcttgt actctttatt aacttttgta aataaatatg aatatgattc
     5461 aaagtcttta ttaattatat cttcaccttt aatgattgag ccaatcgtac catagtcact
     5521 caattcatag ttgtccataa agttaataca ttcatataaa aaattttctt cctcatctcc
     5581 taaaaactgt tctttatatg taataggtgt tttgaccaag tactcttcct catgttcttt
     5641 taaagtaata gtattcgcat ctatattaca ggcattttta gtgaatgaaa tagtagattc
     5701 cattagagct tgaatattat ttagtttttc aatttcattt ttaatatcaa tggttttctt
     5761 ttctaaaagc tcaattagta aattaggtgt tcttttgtct atataagctt taatttcttt
     5821 taaaggcatt tttacttccc taagtgaaga aattatacca aaaatgctaa gttgatggta
     5881 tgagtagtat ctatatccat tttctttctt taattctggt gagaaaagac ctatatcatc
     5941 ataatggaaa agtgtttttt tattgattcc acaaatcttt gcaaattctc cagttgtaaa
     6001 ataatttttt gaaacttgac tcataattat aattcctcct tgactatcct gttacagtat
     6061 agtttatcat agtattttgt aaaggagtgt gattatatga aaaaattaat tcatttttta
     6121 aaaccatata gagttttaat tgttgtagtt ttgatattta cgtttttaca aacacttgga
     6181 actttgtata taccaacact tacagcaaat atagtaaaca atggtgtagt aaagggagat
     6241 atagattata ttgtaaaaac aggtttaata atgatgatag tagctggtat aacagcttta
     6301 tcagctgtgt tagtttgtaa agtatcagca aatctttcat ctggattttg tagagatatt
     6361 agagaagcag tatttgtaaa atcacaagat ttatcaataa atgattttaa taatataggt
     6421 acagcatcaa tgattactag gagtactagt gatattactt taataggtca gtctgtgttt
     6481 atgtttattc aattggtatt accagcacca attataactg tttctggtct attcttagca
     6541 tattctatag ataaagcaat gacaattatt attgttgtag taatgttttt attcatgctt
     6601 tcagcatttt tagttggtaa aaagcttata aagttgttta aaatgatgca aataaagatg
     6661 gacaatatga atagggtatt acgtgaggta gtaactggag taagagttat aagagctttt
     6721 aatagggagc attttgaaaa aaagagattt gatagaactg caatagatta ttctgaaaca
     6781 gcaatttcaa taaataaagt atttgcagta cttatgccta ttgtaatgtt aataatgaat
     6841 ttaggtatag taagtattat ttggtttggt gggatgagag tcagtaatgg aaatatggag
     6901 ataggtcata taatggcact tattgaatat tgtatcttaa ttttattcta tttaatcatg
     6961 ggagtaatgg tatttatgta catacctaga gcaggtgcat gtgcagatag agtaaatcag
     7021 atattagata tagaacctga aattgtagat ggaaatggtc gtaaagatac tgtctcagag
     7081 aggggacatt tagtatttaa aaatgtaaca ttttcatatg cacaatcaga agaacctgtg
     7141 ttaaataata taacatttga agcaaaatct ggagaagtaa ctgcaattat aggaagcact
     7201 ggctcaggca aatctactat tgcaaatata ataccacgtt tttttgaaat tcaatctgga
     7261 gaaatatcta taaatggtca ggatgttaag aagattcctc aaaaagaact tagagataag
     7321 attggatttg tgccacaaaa agctttttta tttagtggaa caatagaaga aaatataagg
     7381 tatggaaaag aagatgcaag tattgaagaa ataaagcatg ctgcaagtat agctcaagct
     7441 gatgaattta tttctgatat ggaagacaag tatgactcat ttgtagcaca aggtggaaat
     7501 aatttgtcag gtggtcaaaa acagagaata tccattgcaa gagcgttagt tagaaagcct
     7561 gaagtatatg tctttgatga tagtttttct gcattagatt ttaaaacaga taaaagactt
     7621 agaaaagcgt tgaaaaatga aatcaaagat tcatcagcta taattatagc acaacgtata
     7681 agtacaatca tggatgctaa tcagattata gttttaaatg atggaaaaat tgtaggtatt
     7741 ggaaaacata aagatttact tgaaaattgt gaagtttata agcaaattgc ggattcacaa
     7801 cttagtaaag aagaacttgc ataaaatgaa gaattaatct gatatttgga ggtgtatgat
     7861 atgagtgaaa aaagaataga tgaacaagat atagaagtac ttagttttga agatggcatg
     7921 tcagcagaaa aagttaagga tgcaaaaaaa actactaaga gattattaaa atatatggca
     7981 aaacaaaagt ttaaaatatt aattattttt atttcagtat tgataagttc agcgcttaca
     8041 gttttagcac ctatggttat gggaaaagct attgaccaac tatttaatgg aataaaaact
     8101 gcagtccaaa caggtacaaa attcagtgtg aattttagta ctatgggggg gatagtatca
     8161 atacttctag ggttgtactt aattagtgca gtatttatat atattcaaca gtatattatg
     8221 tcaggagttg cacaaaatct agttcttagc atgagaaaag atttaagtga taaactcaat
     8281 aaacttcctc ttaagtacta tgattctcat aaaaaggggg aaacacttag tatagtaaca
     8341 aatgatttag aaaaagtagc agatagttta caagaaggat taatgcaact tattacagca
     8401 gtagttacag tagttggttc tattgtaatg atgataagta taagtgtacc acttacaata
     8461 gtatcagcta taacattact tgtttcattg ggtattactg ttgtaattgc aagaaaatca
     8521 caagaaagat tctctgaaaa tcaaaaggca ctaggagagt taaatagcaa tatagaagag
     8581 atatttactg gtcaaatagt ggttaaagca tttagtaaag aaaaggatac tataagaaac
     8641 tttaaaatat taaatcaaaa tctttataat gcaagtagaa gagcacagtt ttcgtcatat
     8701 gcaatatctc caattattag atttataaac ctaattggct atgttattat agcagttgta
     8761 ggtgggatat ttgcatcaac tggagcaatg actcttggta gtatacaagc gtttattcaa
     8821 tatgtaaatc aagcatcaga gcctactaca gaaatatctt atattgtaaa tatgcttcaa
     8881 gcagcaatag catctgcaga gagagttttt acagttatgg atgaggtgga agagataaag
     8941 gatgaagaat cttcaaaggt tattagcatg cctaaaggta aagtacaatt tgagcatgtt
     9001 aaatttggat atagtgatga ttttattctt atgaaagata ttaatatcaa tctaaatgca
     9061 ggagacaaga ttgcaattgt tggacctaca ggagctggaa agacaactct tgtaaattta
     9121 cttatgagat tttatgagat acaaggtgga agaattacta ttgatggtgt taatataaaa
     9181 gacttaaagc gtggagaact tagaactatg tttggtatgg ttcttcaaga tacatggctg
     9241 tttaatggaa gtataaaaga aaatattgca tatagtaagt cagatgccac aatggatgaa
     9301 attgtaagag cagcaaaatc agcaagagta gaccatttta taaggacttt accacaaggg
     9361 tatgacacta tattgactga ggatgcttct aatctatctc aaggtcaaaa gcaacttcta
     9421 acaattgcca gggcaatact ttcagaccca tcagtattga ttcttgatga agcaacatct
     9481 agtgtagata ctagaacaga ggtagaaatt caaaaggcaa tgaataactt gatgaaggga
     9541 agaactagtt ttgttattgc acatagatta tcaactattc gagatgctga tttaatatta
     9601 gtaatgaaag aaggcactat tattgaacaa ggaagccata aggaacttat tgaaaagaaa
     9661 ggattctatg aagaacttta taatagtcaa ttttcaagtg aatatgatga agaagttgta
     9721 tgattatcaa gttttaggca actaaatgat tgataaatat taagttaaat atagtaacat
     9781 gtaaggttat atggaataag agtatttctc tatttcatat aacctttttt atttatcaaa
     9841 ataagataaa attttctttc taaattagat tgaagaaagt atttatcaat ataaaagtaa
     9901 aataatcatt attattagtg aagtaaatat tgtttgccac atatatgcca taaagatgca
     9961 cttattttac ggagatttaa caaagtagtg taaagtatac tctaaatata aaataaatca
    10021 aatagagtta aataaattga gaggtgatat aaatagtgaa taaaaaattg aactatagat
    10081 ttgaaatgaa gcacaaaata actgaagcag atgtgttagc tttaaaatca agactctatc
    10141 caataatgaa gaaagatgaa aatgcatcta aagatggaaa atatcttatt aggagtttat
    10201 attttgatac tcctgaagat aaagcacttt tagataaatt aaatggcgtt gcaataagag
    10261 aaaagtttag gataagattt tataataata actgctctta tataaggtta gagaaaaaaa
    10321 tcaaacatta caacatgaca tcaaagctat caactaattt aaccaaaaaa gaagtaataa
    10381 atatactaaa caatgatatt gaatttctaa aagagtcgac aaattgttta ttaagagaat
    10441 tttatttgaa attaaaatgt gaaagattag aggcaaaaag catagttgat tacaatagag
    10501 aagcttttat ttatccagtt ggaaatgtaa gagttacaat cgatagtgat attaaaactt
    10561 ctgtaaattc gatagattta tttaataaag atttgccgac tgtttcggta attgatgaga
    10621 atatgactgt attggaggtt aaatatgatg aatttatacc agattttata aaggatttaa
    10681 ttcaaattaa taagactact tctacagcag tttcaaagta tgcttcaagt agattatata
    10741 tttaagaata aaggctatga tttatataaa gatatacata aataaggtta aaaaacaaat
    10801 aggttaaaaa taaataaaat aaaaaataaa taagttaaaa ataggaagga tgattttaat
    10861 tatgacattt aatgatattt ttaaatcaag ttttatagaa aacgtatcag gtttttcatt
    10921 tgtggacagt gctttagcac taggttcagc ttttttagta ggtttgttta tatatatggt
    10981 ctataaaaag acttatatgg gaattatgta ttctcgacca ttcaatgtgt cgcttgtagc
    11041 acttacaatg cttacaactt ttgtaatatt ggcggttact tcaaatgtag ttttatcctt
    11101 aggtatggtt ggtgctttat ctatagtaag atttagaaca gcaattaaag acccaatgga
    11161 tttagtattt ctattttggt cattgggttc aggtatagtt ttaggagcag gtttgattcc
    11221 cttagctgtc atgggttcaa taattatggg attgattcta atattctttt caaataaaac
    11281 aatatctgaa actccatata tattgatggt aaattgtaat aatgaagatt cagaagatat
    11341 agcaactgat aaaattaaaa aagtatttac taaatatcaa ataaaatcaa aaagtgttac
    11401 accagaaaaa ggaatagaat tagtgtttga agtaagaatg aaatatggag agacaagtct
    11461 tataaatgac ttgagtcaag tagatggagt tactaatgca gttcttgtaa gctataatgg
    11521 tgactatgta gcatagagag gagtaatctt ttatgaaaga taaaaaattt acccttctta
    11581 tctctattat gattatattt ttatgtgctg tagttggagt ttatagtaca tctagcaaca
    11641 aaagtgttga tttatatagt gatgtatata ttgaaaaata ttttaacaga gacaaggtta
    11701 tggaagttaa tatagagata gatgaaagtg acttgaagga tatgaatgaa aatgctataa
    11761 aagaagaatt taaggttgca aaagtaactg tagatggaga tacatatgga aacgtaggta
    11821 taagaactaa aggaaattca agtcttacat ctgtagcaaa tagtgatagt gatagataca
    11881 gctataagat taattttgat aagtataata ctagtcaaag tatggaaggg cttactcaat
    11941 taaatcttaa taactgttac tctgacccat cttatatgag agagttttta acatatagta
    12001 tttgcgagga aatgggatta gcgactccag aatttgcata tgctaaagtc tctataaatg
    12061 gcgaatatca tggtttgtat ttggcagtag aaggattaaa agagtcttat cttgaaaata
    12121 attttggtaa tgtaactgga gacttatata agtcagatga aggaagctcg ttgcaatata
    12181 aaggagatga cccagaaagt tactcaaact taatcgttga aagtgataaa aagacagctg
    12241 attggtctaa aattacaaaa ctattaaaat ctttggatac aggtgaagat attgaaaaat
    12301 atcttgatgt agattctgtc cttaaaaata tagcaataaa tacagcttta ttaaaccttg
    12361 atagctatca aggcagtttt gcccataact attatttata tgagcaagat ggagtatttt
    12421 ctatgttacc atgggatttt aatatgtcat ttggtggatt tagtggtttt ggtggaggta
    12481 gtcaatctat agcaattgat gaacctacga caggtaattt agaagacaga cctctcatat
    12541 cctcgttatt aaaaaatgag acacacaaaa caaaatacca taaatatctg gaagagatag
    12601 taacaaaata cctagattca gactatttag agaatatgac aacaaaattg catgacatga
    12661 tagcatcata tgtaaaagaa gacccaacag cattttatac ttatgaagaa tttgaaaaaa
    12721 atataacatc ttcaattgaa gattctagtg ataataaggg atttggtaat aaagggtttg
    12781 acaacaataa ctctaataac agtgattcta ataataattc taatagtgaa aataagcgct
    12841 ctggaaatca aagtgataaa aaagaagtta atgctgaatt aacatcaagc gtagtcaaaa
    12901 ctaatacaga taatgaaact gaaaataaaa ctacaaatga tagcgaaagt aagaataata
    12961 cagataaaga taaaagtgga aatgataata atcaaaagct agaaggtcct aggggtaaag
    13021 gaggtaagtc aataccaggg gttttggaag ttgcagaaga tatgagtaaa actataaaat
    13081 ctcaattaag tggagaaact tcttcgacaa agcaaaactc tggtgatgaa agttcaagtg
    13141 gaattaaagg tagtgaaaag tttgatgagg atatgagtgg tatgccagaa ccacctgagg
    13201 gaatggatgg taaaatgcca ccaggaatgg gtaatatgga taagggagat atgaatggta
    13261 aaaatggcaa tatgaatatg gatagaaatc aagataatcc aagagaagct ggaggttttg
    13321 gcaatagagg aggaggctct gtgagtaaaa caacaacata cttcaaatta attttaggtg
    13381 gagcttcaat gataataatg tcgattatgt tagtaggtgt atcaagggta aagagaagaa
    13441 gatttataaa gtcaaaataa gattaaaatt gaatataaat aattttaata ctgttaagtc
    13501 taaatattta cttagcagta tttttttaga aaataatgtg tttgattaat aatataaggt
    13561 aataaattga taaaaattaa atacagcata ttaataatac ataataaagt aataaaaaaa
    13621 ctgtttaagt ataataactt atatttagac agtttttttc atttataaat aatataaatg
    13681 aaattgaaaa catttgaagg atagaaattg gttggataaa tgtataaatt acttcaccaa
    13741 aaaataatga caagagttgt aactaagttg taataaaatg gtagttattt agcataaaat
    13801 aatgtatcat agaagtatat tataaaatta ataaaataag atttttgtaa gaaaactgct
    13861 ggcttttgat atttagagtg agtagttaaa ttatgtttag gaggggtaga atttgttgca
    13921 aaaagtatta tggaaatttt taaagctagt agtcggttta tttatatgtt cagttggtat
    13981 agtattgacg ataaattgta accttggatt atctccctgg gatgtatttc atcaaggact
    14041 ttcaaatcat ataggaataa ctataggaac tgcttctata attgtaggct ctatagtagt
    14101 tattgctgat gtcgttcttg gagaaaatgt aggatgggga actgttttta acatgctact
    14161 aattggattc tttatggatt tacttttata tagtaattta attccagagg ctgatagttt
    14221 atttgtagga ataattatgt tgatattagg attggttctt ttgtctgttg gaatggtatt
    14281 ttatatggga agtggtttag gaagtggacc tagagatggt ttgatggttg caattcaaaa
    14341 gaaaacagga aaatcactta aattaataag aggtactata gaagttggag cattaatagt
    14401 aggattttta ttaggtggaa aagttggtat aggaactata ataagtgcat ttggattggg
    14461 gtatttcaca caaatggtat ttggcttatt taaattagat tgtagtaaga ttaaacatag
    14521 atttatagta gatgatataa aatttatcaa agtgtatatg agtggtgaga aaaaagctgc
    14581 tgttcaaaat atagttaagt aatttaagaa taaattcaac gaaatgttaa gtattttaaa
    14641 ttaacatttt atttagatgg ataaattgaa tattaaaaga atattaaaat atgaggattg
    14701 ctagaacact ataaaatata gatttctagc aattttttat ttgttcaatg tttttttagt
    14761 actagaagtc ttattctcta tttaatgaat gaattaattt ataattagag atactaaact
    14821 ttatagatat taatataaac acctttaaac tgtgtatttt ccaaagcctt taaaatgctt
    14881 gtatttattc taaataaaaa aataaaaaaa tgtcaaaaaa actcttgtat tatatccaaa
    14941 ttttatatat aattacctat gttgtttaga aattttaaac tcaatgttta gtaaacaaaa
    15001 tgtttagtaa aataaaacag aaactaggag gtgcaacatg gatataggtg aaaagataaa
    15061 aagattaaga acagaaaaac aactgaccca ggaagagcta gctaatagat gtgaattgtc
    15121 aaagggattt atttctcaat tagaaaataa cttaacatca ccatccatag ctacccttat
    15181 agatatactt gaaatactag gtacaaattt aagggaattt tttaatgaaa tagatgatga
    15241 aagaatcagt tttacaaaag aagatatgtt tgaaactgaa gatgaagatt taaaatataa
    15301 attaaagtgg ttaattccaa attcacaaaa aaatgaaatg gagccaataa taataacatt
    15361 gtatccagga ggacagtata aagaagaaaa gccacatgaa ggtgaagaat ttggatatgt
    15421 attagcaggt tcaatatatg tccatatagg cgaaaagaaa aataaagtaa aaaaaggaga
    15481 gagtttttac tttagaccaa aagcaaatca ctatatatca aacgaaggca aaactacagc
    15541 aaaagtgatt tgggtaagta cgccaccatc attttagaat agaggtgttt aataattggt
    15601 agaaaatata atagaattaa gggatatatc taaacattat gaggaactta ctgttttaga
    15661 taatttcaat ttagatataa agaaaaatga gtttttaaca ttacttggac caagtggatg
    15721 tggaaaaact acaacattaa aaataatagc aggatttgag tatgctgatg atggtaaagt
    15781 tttatttgaa ggtaaggaga taaataactt gccaccatat gaaagacaag taaacacagt
    15841 atttcaaaaa tatgctttat ttccacacat ggatgtttat gaaaatgtag cttttggatt
    15901 aaaaattaaa aagactccaa aagatgtaat agatgctaaa gtaaaagagg ttttgaaact
    15961 tgttgcacta gaaggttttg aaagacgaca agttgaatca ttgagtggag gacaacaaca
    16021 gagaattgca atagcaagag ctctagtcaa tgaaccaaag gtattattac tagatgaacc
    16081 acttggagca cttgatttaa agttaagaca agagatgcaa atagaattaa agagaatcca
    16141 aaaaaaatta ggaataacat ttgtttttgt aactcatgac caagaagagg ctcttactat
    16201 gtcagatact attgttgtaa tgtataaggg caaaattcaa caaatgggaa caccacaaga
    16261 tatatacaat gaacctaaaa attcatttgt agctaaattt ataggtgaaa gtaacatatt
    16321 tgatggaatt atgatagaag attataaagt taatttctgt aatagagatt ttgagtgtgt
    16381 tgataaagga ttcgaaaaaa atgaaaatat agaagtagta ataagaccag aagatataaa
    16441 aatggtaaag ccagaagaag gaatgttaaa ggggaaagtt acatctacag tgtttaaagg
    16501 agtgcattac gagatagaat tgaatgaaaa tggaagattg tggatacttc ataataccaa
    16561 aaatgctgag gttgggactg aactaggcat ggatatatat ccagaagata tccatataat
    16621 gagaaaagag aataactaag atgaaaagaa aatctttttt agcatatcca tatgtagttt
    16681 ggagtgcgat atttgtaata attcctttaa tattagtggt attttttagt tttacaaaag
    16741 aatcaggtgg aggttatgcc tttacacttg agaattataa agaagtaatt gaccctatat
    16801 atatgaaagt ttttgggcgt tctattcttt tagcaggagg agctacatta atatgcctta
    16861 tagttggtta tccagtggca tatataattt caaaggctcg tgtaagtaga agaggttcat
    16921 taatattatt gtttattctt cctatgtgga tgaactttct tttaagaaca tatgcatggg
    16981 ttgctatatt aggaaaaaat ggacttttaa atacattttt aggttggttt ggaatacaac
    17041 cattagcaat tttgtatact aattttgcta tacttttagg tatggtgtat aactttttac
    17101 catttatggt tcttccaata tatacagcac tatcaaagat ggataatgat ttgataaacg
    17161 ctgctcatga cttgggtgca aataatatga cagtttttag aaaaattata tttcctttga
    17221 gtttgccagg tgtaatgtct ggaataacta tggtatttat gccagcagtc actacttttg
    17281 caatatcaag actgttaggt ggaggtaaaa taatgctggt tggagatttg attgaacaac
    17341 agtttacagt tgttggagat tggaactttg gttcagctat atcgatattt atgatgatag
    17401 taatattaat atctatgtct ataatgtcaa aatttggaga tgaatctgac aaagaaggcg
    17461 gtgggttact attttaaaat taaagaaaat aacttctaat atatatttag cattagtatt
    17521 cttatttttg tatgcaccca tatttgcttt ggtactattt tctttcaatg attcaaaatc
    17581 aatggcaaga tggaatggat ttacatggaa gtggtatggt caattacttc aaaatgagag
    17641 tataatgagt gctttatatt atactattgt aatagctata ttagcgtctg taatatctac
    17701 aatagtagga actataagtg ctataggaat acataagatg agaggtaaga gtaagaaatt
    17761 aatactaaat gtaaactatc ttcctatttt aaatacagag atagttacag cagtagctct
    17821 tatgagcctg tttgtatttg taaaaatgga atttggattt acaacaatgc tattggcaca
    17881 tataatgttt tgtttgccat acgttatact ttcagttttg ccaaaaatga agcaattacc
    17941 tgataatata gaagatgcag ctatggactt gggagcaaca cctatatacg ctcttagaaa
    18001 agtaatttta ccacaaataa aaccaggtat agtttctgga tttttaattg catttactat
    18061 gtcaatagat gactttataa taagtttctt taatgctgga aatggtgtaa gcaatctttc
    18121 tatagaaatc tatggaatgg ctagaagagg tataaaacct gaaataaatg cattatcaac
    18181 aataatgttt gcagtggttc taggtttact tttactagct aataaaaaag aatctatagt
    18241 aagggggatt aaataatgat taagtttaaa aaaacaatat cccttatagc tgtaggtatg
    18301 atgacagtta ctttatttac aggatgtaat aaagcaactg attctacaaa agttttaaat
    18361 gtatataatg ttggagatta tatagatgag tctcttatag ataagtttga aaaagaaaca
    18421 ggaatagatg ttcaatattc aacttatgat actaatgaaa tgatgtatca aaaagtaaag
    18481 agtggtagta cacactatga tttagtattt ccttcagact atatggtaga gaaaatgaaa
    18541 aatgaaggtc ttttggaaaa attagatttt aagaatatac ctaatatgaa atatttagat
    18601 aagagttttt taaatcctgt atacgacaaa actaatgaat atagtgttgc atatatgtgg
    18661 ggaactcttg gaatattgta caataagaaa gaagttaaag accctatgga tagctggaat
    18721 atactttgga atccaaagta taagggaaat ataatgatgt ttgactctgt tagagatact
    18781 atagggataa ctcttaaaaa attaggatat agcatgaata gtgtaaatcc aaaagaaata
    18841 aatgaagcta aaaatctttt aatgaaacaa aaggatttag tattggctta tgtaaatgat
    18901 gaaggtaagg atagattgct tggtggagaa gtggcaatgg gtatgctgta ttctggagat
    18961 gcagttacgc taatggagca aaatcctgac cttgattatg ctatacctaa agagggaact
    19021 aataaatggg tagatgctat gtgtgtacct acaacagcac aaaataagaa agaagctgag
    19081 ctgtttataa actttttatt agacccagag aatgcaaaag taaatgctga gtacattggg
    19141 tattcaacac ctaatacagg agcactaaaa ttattagacc cagaaataac tcagaatcct
    19201 gttgcatatc catctaaaga agtacttgat aaatgtgaaa cttttgaaga tattggtgaa
    19261 aatataaaat tatatgacaa ggcttggata gagttaaaat caaaataggg tcttgttggt
    19321 ttaagtaaat ttacttaata aatacttaat aaaactgttc ttttttagaa aatttatttt
    19381 ctaattattg aacagtttta ttttttgttt aggtcatgtt tttattttga gtttattttt
    19441 atgagaattt aaagtacagt aagtttcatt gattactatt tttataataa taactataaa
    19501 gaggttgtct cgaaattgag ataagcctct ttttcattgt tgaaatgatg aaaacatgtt
    19561 tgatttcata aaaaaagagt gattcatgag ctaaagaatt tgctttgaga cagtattttt
    19621 tattaagtta tattcatatg caatttaaat agtttattaa taaatgaaaa cttatttgta
    19681 taatgatttt aataataatt agaaaaaatg ggcaacttaa tagaataatt ggaggtgtaa
    19741 atttataaaa aatgaatagg ataaataaaa aaatagattt attaatattg gctttgctcg
    19801 tttttatatt tttaattgtt ggaatagtaa tactatcgat taggacagaa aataaattga
    19861 atgtgttcat aatgttagca ataatttttt ttataataat gttaacttat ttatcaaatt
    19921 cagtagtggg gttgataaca agttcaataa ttatatttat gtatacatcc tatattttat
    19981 ataacaatat tactcacaat atggatgttg aatttatatc atatatgtgg ataatagcaa
    20041 caccagtatc ttcaattata atgggtaatc tgaacaaaag cataaatgaa ttgcaaaata
    20101 caaataaaaa actatctgaa cagtataagg aattagtgac tattgatagt gaaactggac
    20161 ttagaaattt aaagattttt tataatgatg taaatatgga gataagtaaa tctattagac
    20221 ataatactga tttttctctt atgatagtaa aattaccata ctacggaaac ctacagacta
    20281 tatttggtga aaacaaaact aataaaatag taaaatatat tggttcaaat attatagaat
    20341 gtacaagaaa tgaagatata atttatagtt tacaaaaaga tatgataggg atactaatgc
    20401 caaatacatc tttggaaggt tcgaaagttg ttaaagacag aataaagaaa aggataaaag
    20461 aattaaattt agatttaaac aataggggaa aatatgtaaa tatagatgtc aaaattgcat
    20521 ttttacaata taagagtagt tttggagata gtataaattt taagaatata gttgaagagg
    20581 aacttcaata tgatgtttaa aatatttaat aaaagtttat taaaattagt aattgtatta
    20641 tcaatagtac tgacaatatc aaattttagc ttattttcat atgcatcaga aattacttcc
    20701 agcacagata aggtattaat catatatgat tctaagaaag aaacagctta taatagagat
    20761 attttaaata ttatgagaac tctattggga agatttagtt cagatataga gctattaaaa
    20821 ttgagtaact atgatggtga gataaatgaa aattattaca gtcatatatt tattttagga
    20881 atcaatgaaa actcatataa taatgataaa aacactaaaa acttgattag ttccttaaat
    20941 tcatataaag taactatatg ttggttagga tatggtatag aaaatctttt ggaacataaa
    21001 aaatacaatt tagactatgt aggaaaaaca aacaatatag ttagtgtaaa ttatagaggt
    21061 aaatcatata acttggatga acattatgtt tttaacattg tagaatcaaa agatacaagt
    21121 aataaagtaa taggaagtat taatgatacc ttaaataagt atccatatat aataaatgat
    21181 aaaaatcttt tttatgtatc aaaattagat ttagatggag ttttatttta tatattttgc
    21241 gattcactta atgatatttt taatattaag acgtttgata aaggacgtat atttgtaaga
    21301 atagaagatg tacatgcttt tagagaacca aaaaatcttg tagagatagc ggattattta
    21361 agtagtaaga atataccatt tacgattgca ttaataccag catatgtaaa tcctaaaaat
    21421 cataaagtaa taactttatc agaaagccct gaaattgtca aagcaataaa atatatgcaa
    21481 gataaaggtg gaactgtgat attgcatgga tatactcatc aatacaagaa agaagaagtt
    21541 tctggagaag ggtatgaatt ttgggatggt aaaaaagatg aacccttaaa agaaaatatg
    21601 aagatatttg tcaaagatag agttttgaat ggtcttagag tatgtataga gaatggaatt
    21661 tatcctcttg catttgaagc gccacattat gcaatggaat cagagggata taaagaatta
    21721 aaaaaatatt tttctactta tatggggcaa catcaaaata atgataaaaa atttagcaca
    21781 aatacatatc cttatattat tagggataca gaggagttta atatatttat acccgaaaat
    21841 ttaggatata tagacccaga agacaagttt acttttcaaa atataaaaga aaatttagat
    21901 aaattatcta ttgtaagagg ttttagtgga gggttcttct ttcattcata tttaaacata
    21961 gagtatctaa aaaacacaat agaatattta gaaaaacaga atatagaatt tatgaattta
    22021 agagatttta ataattgggt aaaagttgat gaaatacaaa taagaaataa tggagatgaa
    22081 attatagtca actatgataa ggacttagat gaaataacta aaagtgatac tagatttaaa
    22141 tacatatcca atattagtaa aattttaata ttcatagtat ctataagtgt tttaatattt
    22201 gtgattatat ttatatattt taagagaata gat
//
LOCUS       DAEPOG010000052        22134 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEPOG010000052 DAEPOG010000000
VERSION     DAEPOG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.052"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            102..278
                     /locus_tag="KR432_001638"
     CDS             102..278
                     /locus_tag="KR432_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640179.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            452..1582
                     /locus_tag="KR432_001639"
     CDS             452..1582
                     /locus_tag="KR432_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0640180.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1823..2173
                     /locus_tag="KR432_001640"
     CDS             1823..2173
                     /locus_tag="KR432_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0640181.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2181..2630
                     /locus_tag="KR432_001641"
     CDS             2181..2630
                     /locus_tag="KR432_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH0640182.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2732..3307
                     /locus_tag="KR432_001642"
     CDS             2732..3307
                     /locus_tag="KR432_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0640183.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3300..4709
                     /locus_tag="KR432_001643"
     CDS             3300..4709
                     /locus_tag="KR432_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBH0640184.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4893..6326
                     /gene="eutA"
                     /locus_tag="KR432_001644"
     CDS             4893..6326
                     /gene="eutA"
                     /locus_tag="KR432_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH0640185.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6344..7708
                     /locus_tag="KR432_001645"
     CDS             6344..7708
                     /locus_tag="KR432_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBH0640186.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7721..8602
                     /gene="eutC"
                     /locus_tag="KR432_001646"
     CDS             7721..8602
                     /gene="eutC"
                     /locus_tag="KR432_001646"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBH0640187.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8622..9275
                     /gene="eutL"
                     /locus_tag="KR432_001647"
     CDS             8622..9275
                     /gene="eutL"
                     /locus_tag="KR432_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH0640188.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9286..9987
                     /locus_tag="KR432_001648"
     CDS             9286..9987
                     /locus_tag="KR432_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0640189.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9990..11459
                     /locus_tag="KR432_001649"
     CDS             9990..11459
                     /locus_tag="KR432_001649"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBH0640190.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11555..11842
                     /gene="eutM"
                     /locus_tag="KR432_001650"
     CDS             11555..11842
                     /gene="eutM"
                     /locus_tag="KR432_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBH0640191.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11969..12730
                     /locus_tag="KR432_001651"
     CDS             11969..12730
                     /locus_tag="KR432_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBH0640192.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12743..13372
                     /locus_tag="KR432_001652"
     CDS             12743..13372
                     /locus_tag="KR432_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0640193.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13414..14139
                     /locus_tag="KR432_001653"
     CDS             13414..14139
                     /locus_tag="KR432_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBH0640194.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14152..14424
                     /locus_tag="KR432_001654"
     CDS             14152..14424
                     /locus_tag="KR432_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBH0640195.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14417..14968
                     /locus_tag="KR432_001655"
     CDS             14417..14968
                     /locus_tag="KR432_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0640196.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14980..16071
                     /gene="eutH"
                     /locus_tag="KR432_001656"
     CDS             14980..16071
                     /gene="eutH"
                     /locus_tag="KR432_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0640197.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16073..16546
                     /locus_tag="KR432_001657"
     CDS             16073..16546
                     /locus_tag="KR432_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBH0640198.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16666..16767)
                     /locus_tag="KR432_001658"
                     /pseudo
     CDS             complement(16666..16767)
                     /locus_tag="KR432_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17062..17583
                     /locus_tag="KR432_001659"
     CDS             17062..17583
                     /locus_tag="KR432_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0640199.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            17962..19137
                     /locus_tag="KR432_001660"
     CDS             17962..19137
                     /locus_tag="KR432_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640200.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19172..20710
                     /locus_tag="KR432_001661"
     CDS             19172..20710
                     /locus_tag="KR432_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0640201.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20742..21944
                     /locus_tag="KR432_001662"
     CDS             20742..21944
                     /locus_tag="KR432_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0640202.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
BASE COUNT         8974 a         2484 c         4124 g         6552 t
ORIGIN      
        1 ataaaatcat aggttagtgt gaaaaagttg aaatttaaat attttaaaag ataatttttt
       61 ggaactaata ggtataacaa aaatttatag ggggattgca tatggaaatc aatgttatag
      121 aaattttccc taaagataaa gctaaactta ataaaataga aatggataaa gctagttggt
      181 ttgtaaatat aataagtaaa aaatatccta aagaagcttt aaatgaagca tttagtattt
      241 tagaaaaaga attaaatata agtaaagcta atacataagc tttgattatt gattttatat
      301 actattggtt ttatacgcta tgacttgaca ttatattaat caaatagtat atttttaaat
      361 atagcacaat gaagtgttaa aagagcaatg gagctccata gataaagtct atggagcttt
      421 tttgcgtatt taacttaagg aggatagagc tttgaataac tttgagatta aaactagaat
      481 aaaatttgga gaaggttcac taaaagtttt gcaagaatta aaaaacaaaa atgtattaat
      541 aataacagac ccttttatgg tgaaatcaaa aacaatagat aaaataacat ctaatttaat
      601 aaactcaaca tatcaagttt ttgaagaaat agtaccagac ccaccaatag aagtagtagt
      661 agcaggaata gaagcattta aatctataaa tccagacgta ataatagcat taggtggtgg
      721 ttctgcaata gatgcagcaa aatctataat ggacttttct aaaaaaatat taaaaattaa
      781 cgaagtagag tttatagcaa taccaacaac aagtggaaca ggttcagaag taacatcatt
      841 tgctgtaata acagatagtc aaaaaaaatg taagtatcca ttagtgagtg atgatttact
      901 tccagatatg gcaatactag acccagagct tgtaaaaact gtaccaaatt ttataacagc
      961 agatacaggg atggatgtat tgacacatgc tatagaagct tacgtatcta caaattctaa
     1021 tgacttttca gatgcactag ctgaaaaatc tataaagtta gtatttaaat acttaataaa
     1081 agcatataaa gatggaaatg acatagaagc tcgagaaaaa atgcacaatg catcttgttt
     1141 agcaggtatg gcatttaacc aagcatcact tggcataaat cattctattg cacatgtatt
     1201 aggtggaaaa ttccatatac cacatggaag aacaaattct atattacttc ctcatgtaat
     1261 agaatataat tcatcaatag taggatatac taacacaact tactctgata cagctaaaaa
     1321 atatgcacaa atagcagaat tagtaggact tgggagtgct aatataagaa tatctgttag
     1381 aaatttagta aatgaaataa agaaactaca aaaagaaatg aatatgccaa ctaaattgac
     1441 acaatgcaaa ttaagtttag aagatataac taatgaagaa agagaaatag caaaacttgc
     1501 tataaaagat acttgtacat taactaatcc taaggtacca actgaagatg atataataaa
     1561 catattaaat aacattaaat aactacattt tattacatgt aaatataata ttatacaaat
     1621 agggaaaatg tttttaaaaa tataaattat atattgacaa atataaaaca taaaactata
     1681 atgaaagtat cacaaaggcg tgatacttta gtagaaagca atggagcttc ttaggaatat
     1741 taatattatt cttatggagc tccttttatt ttaagcaaat agctttttaa taaactatat
     1801 tcaaagaaag cagggaataa atttgactga agaatctaag caaagagtta ttcaagaata
     1861 tgttcctgga aaacaggtta cattagcaca tatcatagct aaccctaatg aagacatata
     1921 taagaagtta ggattagtac ttgataaaag agatgcaata ggaatactta caataacacc
     1981 aagtgaagcg tcaataatag cagcagatgt tgctacaaag gcttcaaatg tatctttagg
     2041 ttttatagat agatttagtg gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc
     2101 agcgttaaat gatgttttag aagtcttagg aaatatgtta aacttctcat ctacaaaaat
     2161 cacaaggaca taataataca atgaagaata taatatttat gggaaagaca ggtagtggta
     2221 aaactacctt atgtcaaaaa ctagaccagt tagaattaaa gtataaaaaa actcaatcag
     2281 tggaacttta taataatgca atagatactc ctggtgagta tatggaaaat agaaacctat
     2341 ataatgcctt aataacaaca tcagcagatg ctaaaattat agcaatagtg tatgatgcca
     2401 cacaagaaga aaattatata gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag
     2461 gaattataac taaaataaat aaagctactg aaaacgaagt caatatagct tatgaaagac
     2521 tatcactagc aggagcaagt caaatcttta aaatagacac agttgatgat attggacttg
     2581 aaaaattatt taaatatcta gaaaaatcta aaaatataga tgaagtttaa ggggaaatat
     2641 ttttttattc aaaaatatta ttaaagtatg ataaaataaa aaaatgtata aactatttta
     2701 agaagattta atctttaagc gaggtaggaa catggggaaa aaaatactat tggttgatga
     2761 tgaaccactg ttaaggatgg atacaaaaga tatattagaa agtcatggct atgaaataat
     2821 aggcgaagca agtgatggat ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt
     2881 agttataatg gatatagaga tgccaatact tgatggtata aaggcaggca aaataatatg
     2941 taaagaaaat ctagctggtg gagtactttt attaacttca tttgaagata aagaatacgt
     3001 agaaaaggct aaaagtatag gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc
     3061 acttatccca accatagaaa tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa
     3121 agatttagaa aaagtaaatt ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg
     3181 tatacttata agagagttta agatttctga ggatgaagca tacactagaa taagaaaatt
     3241 aagtatggat aaaagaacta caatgatgga aattgccaaa atgattataa taggatacga
     3301 tgactaatac gataaaaaaa atatcaactg cacaaacata cttaaataat aacgaaattg
     3361 aaaaaattat agaagtatca aaatccttag atttaatggc ttctttttat gaggctgatg
     3421 tttttataga tgtactaagt aaatttgatg atgaagcaat tgttgtggca catggtagac
     3481 ctaaagaaaa gtcaatgtac tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg
     3541 aaccaggagt tataaagtgc ttaaaaacag gagaaagagt aagagatatt aaagctttaa
     3601 cacaggaata taaactggtc aaacaaaata ttcagccaat aacactagat gacaaagttt
     3661 tagcagtact aatagttgaa aaagatataa gtagtgaagt taagaatgag tttaatgcta
     3721 gtgaagacga agaaaataaa aatttacatc agtttataaa acttatcgga aataatgact
     3781 ttttagttga taatttaaat tcagccatat taatatttga taaaaatgga aaattaaaaa
     3841 ttaaaaatcg tattgcaact gagatataca aaaatcttgg gtttggatat gatatacaag
     3901 atatgcatta caatgattta gatatagaaa ccaacacctt tgaaaatata ttaatggatg
     3961 aaaattatga tgagacaaat gaagtttcaa taggtaatat gttttttaaa ataaagacca
     4021 tatacatcaa agatgatgaa tttaaggttt gcaaaatagt acaagatata agtgatttaa
     4081 agagaaaaga agaagaatta gttttaaaaa cagtggctat aagagaagct catcatagag
     4141 taaaaaataa tcttcataca gtaatatcta taataaaaaa acaaagtaga ttatctcaaa
     4201 atgatgaagt aaagcagtgt ttagataaca taataaacag agtatttgca atactttcaa
     4261 ctcattattt actatctaaa gaagttgata ataatatatc tatacgagaa ggtatcaatt
     4321 tattagtgtc aaatattcaa ggtggctatt tatttagtaa ggatatagat atacgtataa
     4381 ctggtgaaga ttttaaaatt agtggagaaa aagcaacagc aatacttttg gttataaatg
     4441 aaatactaca aaattgttat gaccatgcat tttcggatag agaacatggt actatacaaa
     4501 tattaataaa taaagataac gactatagaa atattgctat tatagatgat ggaattggat
     4561 ttgatagtaa taatataaat aaaaatagct tagggacata tataatagat agttatataa
     4621 cacagatgtt aaaaggaaat atagagagaa aaagtaatga gaatggtaca gaagtattaa
     4681 ttactatacc tacacaaatt aatatttaaa taataactca tctacaaagt agtagtttta
     4741 attgagcaaa gaggcttaag gttattaata gattaagtta attgaatgta tttataaaaa
     4801 ttataagtat atattcaatt taacaggtat ttattataac tttaagcctc tttgcttgtt
     4861 taagacatac ttaaatttta ggaggtgaga aaatgagaga agagttatta agtgttggta
     4921 ttgacattgg aacaagtact actcagttaa ttttttctaa attaatagta gaaaatactg
     4981 aatcaaattt ttcggtacct agaataagta tagtagacaa agaaattatt tataaaagca
     5041 aagtttattt cacccctctt atttcaaata atgaaataaa tggtcaacaa ataaaagata
     5101 tagttgaaaa tgaatataaa aaagcaaata tatctaaaga ggatatacat acaggagcag
     5161 taataataac tggtgaaaca gctagaaaag aaaatgcaaa tgatgtactt catactttga
     5221 gtggatttgc tggggacttc gttgttgcaa cagcaggacc tgatttagaa agtataatat
     5281 caggaaaagg agctggagct catatttatt caaaagaaca ttcaactaca attgtaaata
     5341 tagacatagg tgggggaact agtaacatag cctcatttaa aaggggagaa gtaaccgata
     5401 ctgggtgttt ggatataggt ggaagacttg tgaagataga taaaataagt aaaaaaataa
     5461 cttatatatc acctaaaata gataaaatca taaaagataa taatttaaat cttcaagtag
     5521 gcacaatggc tactgtagaa aatacaaagc caatagtgga tataatggtt gacttattaa
     5581 tggaaagcat aggattaaag aaacagacac ctttgtttga ttttataata accaataaat
     5641 caataagtat agatgaaaat attaaaaatg tttcattctc tggtggagta gctgattata
     5701 tttactatga tggagaaata aaagattatt ttgaatatgg tgacttggga attttacttg
     5761 ggcaaacaat aaaaaattgt gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa
     5821 caataagagc cacagtggtt ggggcaggtt ctcatactac tgaagtaagt ggaagtacaa
     5881 taacttacac tagcgaaaag tttccactta agaacttgcc agttttaaaa ttatcagttg
     5941 atgaagaaac aggtacttca aaacaaatca gtgaagctat aaaaaataaa cttaattggt
     6001 ttaaactaga aaatgatttg cagaaagttg ctatagccat gataggaaag aaaaatccaa
     6061 cctttttaga tatacaagag tatgctaaag ggtttgtaga tggaatgcaa caaatcatag
     6121 aaaatgattt accttttata gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag
     6181 caatatatgc attattaaat tatgaaaaag aaattgtttg tatagacagt gtcaaggttg
     6241 aaaatggaga ctatatcgac attggaaaac ctatagttga aggtaaagtt ttacctgtgg
     6301 taataaaaac attagttttt aattaataga aggaggtaag taaatgattt taaatacaaa
     6361 attatttgga cgtacttatc agtttaaagg cttatatgat gtaatggcta aagctaatga
     6421 agaaaaatca ggagataaat tagcaggtct tgcagcagaa tcagctgaag aaagaatagc
     6481 agcaaaagtt gttttatcac acataaaatt ggaggatata agaaataatc ctgcagtggc
     6541 atatgaagaa gatgaagtaa caagaataat acaagatgct gtaaatgaaa aagtttatga
     6601 agaaataaaa aattggacag tatctgaact tagagaatgg ctattggatt caaatactac
     6661 aagtcttgat ataagaagaa tatcaagagg attaacttct gaaatgatag cagcagttgc
     6721 aaaattaatg tcaaatatgg acttagttta tgcagctaaa aagataaagg ttactgctca
     6781 ctgtaataca actataggag aacaaggaac attatcagca agacttcaac ctaatcatcc
     6841 aacagacgac ccagatggaa tacttgcatc acttcttgaa ggtttaactt ttggtatagg
     6901 agatgcagta cttggattaa atccagttga tgactctgtt gagagcgtta ctaaagtatt
     6961 aaaaagattt gaagaagtaa aacaaaaata tcagatacca actcaaacat gtgtattggc
     7021 acacgttact acacaaatgg aagcaataag aaatggtgct cctgcagatt taatattcca
     7081 atctatagca ggttctgaaa aaggaaatga agcatttgga tttaatggcg acactataga
     7141 agaagctaga cagttagctt taaaacacgg tacagctaca ggacctaacg ttatgtattt
     7201 tgaaactgga caaggttcag aattatcttc agaagcacat catggaatag accaagttac
     7261 tatggaagct agatgctatg gatttgctaa aaagtatgaa ccattcttag taaatacagt
     7321 tgttgggttt ataggacctg agtacttata tgatgctaga gaagttgtaa gagcaggact
     7381 tgaagaccac tttatgggta aactacatgg cttaccaatg ggtgttgatg tttgttacac
     7441 taaccatatg aaggctgacc aaaatgatat ggaaaactta gccatactat taacaacagc
     7501 aggatgtacg tattttatgg gaatacctca tggagatgat gttatgctta actatcaaac
     7561 aacaggatac catgaaacag catcacttag agaaatgttt ggactaacag caataaaaga
     7621 atttgatgct tggatggaaa agatgggatt ctataaagat ggtaagttaa cagagttggc
     7681 gggagacgca tctgtattat taggatagga ggtatttaat atgaatgaga aggatttaaa
     7741 agcattggta gaacaattag ttggacaaat ggttggagaa ttagatacta atgttgtatc
     7801 tgagactgtt aaaaagacaa ctgaagttgt agtagacaac aatgctttta tagatgatat
     7861 aacagaagtt gatataagaa agcaattact agtaaaaaat cctaaggatg cagaagctta
     7921 tttagatatg aaagcaaaaa cacctgcaag attaggtata ggaagagctg gtactagata
     7981 taaaacagaa acagttttaa gatttagagc agaccatgct gctgcacaag atgctgtatt
     8041 ctcttatgta gatgaagaat ttataaaaga aaataatatg tttgcagtgg aaactttatg
     8101 taaagataaa gatgaatact taacaagacc agatttggga agaaaatttt ctcaagaaac
     8161 tataaataat ataaaatcta aatttggtac aaatcaaaag gttttaatat tagttggaga
     8221 tgggcttagc tcagctgcaa tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca
     8281 gggtctaaaa atgtatggta tagattcaag tgaaatttta tttgttaaac attgtagagt
     8341 tggtgctatg gaccacttag gtgaagaatt aggttgtgaa gttatatgta tgttggttgg
     8401 agaaagacca ggattagtta ctgctgaatc aatgtcagca tatatagctt ataagccata
     8461 tataggaatg gcagaagcaa aaagaacagt tatatcaaat attcacaaag gtggaacaac
     8521 tgcagttgaa gcaggtgctc atatagctga acttataaaa actatgttag ataaaaaagc
     8581 ttcaggaata gaccttaaat aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc
     8641 gtcctaatat actaggaatt aaaataattt cgaatataag tccagaaatg gcacaaaaac
     8701 tagaattaaa atcacatcac aaaagtttag gtttaataac tgcagactgt gatgatgtta
     8761 cgtatacagc tcttgacgaa gcaactaaag cttcagaggt tgatgttgta tatgcaaaat
     8821 caatgtatgc aggtgctgca aatgcttcaa caaaacttgc tggtgaagta ttaggaataa
     8881 tagcaggccc aagcccagca gaagtaagaa gtggtctaaa tgcagtagtt gattttctag
     8941 agtatggagc aacttttata agtgcaaatg atgatgattc aatagcatac tatgctcact
     9001 gcgtatcaag aacaggtaca tacctttcag agatagcagg aataagagaa ggtgaagcat
     9061 tagcttattt agtagcacca ccacttgaag caatgtatgc actagatgca gcaatgaaag
     9121 ctgcagatgt taagatgtgt gaattatttg cacctccaac tgaaacaaac tttggtggag
     9181 cattacttac aggttctcaa tcggcttgta aagcagcatg tgatgcattt gcagaagcag
     9241 taaaatcagt tgcagacaat ccaacaggat tttaaggata gttagatgga tgctataggc
     9301 ttaatagaag taataggata tgttgctgca atagaagctt ctgatgcttg tttaaaatct
     9361 gcaaatgtta atattgtaag aattgataag gttggggcag gtattgttac tttaacaata
     9421 tgtggagatg tcggtgctgt aaaagctgct ttagaagcag gtgaaatagc agcttccaga
     9481 gttggtacat taagaacatc acatattata ccgagaatcc ataatgaagt aacaaatgct
     9541 ttattcaaaa ctaaagaacc taaagtctgt gaagtttcag aagataaaga aaaaaacttt
     9601 gagattacat tggaaataac tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta
     9661 attttagatg tcaatgaaaa cagtggagat actgaaaatt tatatagtga tatagataaa
     9721 acaaacaaaa aaattgaaac agttgatgat agtttaacag aagctaatga ggaaaaaata
     9781 gaaaatacta atgataaaga agtattggaa caagttaaaa tagaaaattc cgattttgat
     9841 agagatgacc tatcaaagaa cagtgtaaaa aaacttaaag ctatggctaa gaaattagat
     9901 tcttcaataa cttataaaga tttaaatgtt cttaaaaaag aagaattaat aaacttagtt
     9961 aaaaagctta gcagaaggga tagatagtta tgacactttt agataaagat ttagtatcta
    10021 tacaagaagt cagaactctt gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa
    10081 tgagtcaaga gcaaatagat aaaatagtaa aagctgttgt agatgcagca tttaataatg
    10141 ctactaaatt agctaaaatg gcaaatgaag aaactggatt tggaaaatgg gaagataaag
    10201 taattaaaaa tacttttgca tctatgggag tttatgaaac tttaaagaac acaaaaacta
    10261 ttggaataat tggtgaagat aaagaagcca atacgataga tatagctaca ccagttggag
    10321 ttgtggcagg tcttatacct tcaacaaacc caacatcaac agtaatatac aaaactataa
    10381 tatctctaaa agcaggaaat actatagttt tctctccaca tccaaatgca aaaaactgca
    10441 tacttgaaac agttaatata ataagtgagg ctgctataaa ggctggagct ccagaggatt
    10501 caatattatg tataactaat ccaagtatag ttggaaccaa tgaattgatg aaacataaag
    10561 atacaaactt aatactagca actggaggag aagcaatggt tagagctgct tactcttcag
    10621 gaacaccagc tattggagtt ggaccaggaa atggaccagc atttatagat aaatcagcaa
    10681 atgttaaatt agcaatcaaa agaataatag attctaaaac ttttgataat ggtgttatat
    10741 gtgcatcaga gcaatctata gtaatagaaa aatctatgga agaaactgtt ttaaaagagc
    10801 ttaaaaatca aggtgcatat gttttaaatg aagaggaaag tgaaagatta gcaaaattca
    10861 tattaagagc taatggtact atgaatccac aaatagttgg taaaagtgtt gaggtaatag
    10921 caaatttagc agacttaaca aatataccta aaaatacaaa agttctagtt gcaagagaaa
    10981 ataaagttgg tcacaatgta gcctattcaa gagaaaaact tacaccaata ctagcgtttt
    11041 atgttgaaga aaatgtagaa gctattatga acagatgtag agaaatattg ttaaatgaag
    11101 gtgcaggtca tacattctca atgcatgtaa atgatgaaga attggtaaaa agatttgccc
    11161 ttaatatgcc agtatcaaga atacttgtga atactccttc agcacttgga ggaataggtg
    11221 catcaacaaa ccttgtacca gcactaacac ttggttgtgg tgcaataggt ggaagttcaa
    11281 cttcaaacaa tataggacca cttgatttaa taaatgttaa aagagtagct tatggaatca
    11341 aagaaataga agaacttgga agagataaat ctcaagttga aaaatcaatg tttgactcaa
    11401 tggataaaga agaattaata aatcttcttg ttcaaaaact agtcaaagaa ctagcataac
    11461 taaaataaac aatgaaactt atggctatcg ctataaattt ataaaaatat aaactataaa
    11521 aaactataaa attaaatttt tggaggataa gattatggca agtgcaaacg cattaggaat
    11581 gatagaaact aaaggattag taggagcaat agaagcagca gatgcaatgg ttaaagctgc
    11641 aaatgtacaa ttagtaggaa aagagcaagt aggtggagga ttagttactg taatggtaag
    11701 aggagatgtt ggagctgtta aagctgctac agatgcagga gctgcagctg ccgagagagt
    11761 tggagaacta atatcagttc atgttatacc aagaccacac tttgaagtag atgctatatt
    11821 accaaaggta tcagctgagt aatataagaa taattttaaa atttaaattt tgattgaaaa
    11881 agaggctatc tcaaaaatga gtcttactaa taaactcaat gtattttgag gtagtctttt
    11941 ttatacatct aataggagat gtgataaaat gggtgtttta acagaaaacg aagtaaaaag
    12001 aaaaattgga aaaaacaaaa ataatataac tgaattttat atagaaaaag gtcaaataat
    12061 aactccttct gcaaaatctt atttagctga gagaagtatt gagcttaaat atgttgataa
    12121 actttcagat gtaaacaatg aacctaaaga agttgtaaaa gaagtagtta aagagaaaaa
    12181 atataaatat actactgtat ttggcgctaa attggatgaa aaaccagaac atatgactca
    12241 attaaatgga aatcttttag tttttaaaga ccataaaaga ataatattga gaggaaaaat
    12301 agattctctt gaatcagaaa tactaaaggt acagatactt tgtcagaaac tagatatgcc
    12361 aaagcttgtt aaggaattgc aagaagtatt agactttgta agaaatataa tgagatgtga
    12421 agttttagat gaaaagctag aagactttaa acttttagga ttaactcctc aagaacttag
    12481 agaacaatct cactatccta aaaaatactt tggtatagga catgaattcc caagttatga
    12541 aatgggagaa gtagtagtag ctataaatag tataagaagt aatacacgag aggttgaact
    12601 tgcagcctat gaagcattta agggtgagta tggaaatgtt cacagagaag atttaataaa
    12661 ggcacttaat agattatcgt cagtattttg gataatgata tataaggtta gaactaataa
    12721 gtataagtag ttggaggaca taatggagaa tctattaaat gaaatagtgg aagaagtaat
    12781 aactagagtt aaaaaagaag catttataga agttgaagca tcaggaagac acgttcactt
    12841 aagtagagaa gatgtcgata aattatttgg agaaggatat actcttacaa aactaaaaga
    12901 cctatctcaa ccaggtcaat atgcatgtaa ggaaagagtt acaataactg gtcctaaagg
    12961 aagtataaaa aatgtagttg tacttggacc atgtagaaat gaaactcagg tagaaatatc
    13021 tttaacagat ggttcaactc ttggactaaa agctcctata aaacaaagtg gagacttaga
    13081 aggtacttta agtattaaaa tatcaacaca atatggtgaa gttgaacttg ataaggggtt
    13141 aatggttgct aaaagacata tacacatgac tcctaaagat gctgaaaaat tcaatgtatg
    13201 tgataaagag atagttgaag ccaaggtgat gggtcaaaga cctttaacat ttgatgatgt
    13261 agtaataaga gtgagtgaca gttttaaaac ttatatgcat atagactatg atgaagccaa
    13321 tgcatgtggt tattcaaaag gaactgttgc taaaataata aagaaagcat aaatttaacc
    13381 tactatataa attacctcta gggaggattt aatatgaatt atgataacct tgtagattta
    13441 ataacggaag aaatctataa aaaaataaat agtaatgaaa taaaaatatc taataaacct
    13501 aaagctgtga ttgtatttga acaagataac aataaattta acttattaaa agatgaattt
    13561 gaaatactag aatttgataa aagtataagg gaatgtgaaa tagtgatagt ttcaagactt
    13621 tgtatgaggg ggctatcgaa tatagcactt ggaaattcaa caagtgatga agaaagattt
    13681 atactaaaaa tgattatgaa aggcaaaaaa gtatatgtcc tagatgaagg aattgaatac
    13741 aaaaaatata aggatactgc accaaaagca ttatataaga aatttatgtc atttgaagat
    13801 gaaatatgta aatttggtgt agaaattata aaagacttaa atgttataac taaaaataag
    13861 ttaaatataa agaaagaact agaaagtact agaggttcta aaaaagaaga tacaaaagtt
    13921 ttagataaag atgttttaaa tttaaccagt gtagaaagtg taaaaataga taatgaattt
    13981 agtttggatt taaggaataa aaaacttata tcggaagctg acttaagaaa accaactata
    14041 aatggagtta aaaacatatt agttaataaa aaaagtataa taacaccatt agctgtagat
    14101 tttatgagaa tacaccatct caaactaaag aaactgtaag gaggtaattt tatggatata
    14161 ggaaaagttg taggtaatgt ttgggctaca agaaaagatg aaaaattatg tggccaaaaa
    14221 cttttagttg taaaaatttt aaaaactaaa gacacataca aggaaggctt catggttgca
    14281 gccgataatg caggagcagg aaatggagac ttagttctta tatccaaagg ttcctcagct
    14341 agagagtcga ttgaaaaatc tcatgttcca gtagatgcaa ctatagttgg agtagtcgat
    14401 tcgtttgagg tgtttgatga ataaaagtat aggagctata gagtttaaaa gcattgctaa
    14461 aggtatagaa gtatccaatg aaatgataaa gaaatcttca gttgatgtat tgtacttaaa
    14521 aagtatttgc ccaggtaaat ttctaataat agtaggtgga gaaacttctt atataaatga
    14581 atgtgtagat tatggaataa aacttggaga aggatatatt gtagataact tcgtaataaa
    14641 tgcaatttca caagaaatat tagatggatt taaaaataag tatcaaaagc tagatagtat
    14701 agtctctata ggcgttgttg aaagtagtaa agtatgcgct ggaataaaaa tgcttgataa
    14761 gactttaaaa tctggcgact tagtattagt aaaacttcaa ttatcttttg ctataggtgg
    14821 caaattagta tatgtagtag caggagattt aagtagttta gaatatgcat taaaagaaag
    14881 tgaaaatgta gtaagagaaa aagaagtaat atataagact gtaataccat cagtagacag
    14941 tcaaataata aaaagtctaa taaaataatg gggggaatta tgagtataaa cgaaattata
    15001 atctatgtaa tggtagtctt catggtactt ggagctatag ataaatgtat aggtaacaaa
    15061 tttggtcttg gagaacaatt tgaagaaggt ataatggcaa tggggtcact tgcagttgca
    15121 atggttggtg ttatatgttt agcaccagtt cttgctgatg tattaagacc aattgtagta
    15181 ccagtattta atatacttgg agctgaccca gctatgtttg ctggaagttt attagctaat
    15241 gatatgggtg gtgcgccact tgcacttgaa cttgcgaaag accctaattc tgggttattt
    15301 ggtggattaa tagtaggggc tatgatggga cctacaatag tatttataat accagttgcg
    15361 cttggaataa ttgaaaaaga agaccaaaaa ttcttagcaa caggaatact tgcaggtata
    15421 ataacaatac ctattggagc attcgttggt ggattagttg caggattcga ggtaatgatg
    15481 gttcttagaa acttagtgcc aataataata tttgcagtag ttatagcact tggattatta
    15541 aaattcgaaa atgctatgat aaaaggattc acatactttg gaaaaggtgt agtaatagtt
    15601 ataactttag gacttgctgc tgccatagtt gaagctttaa caggaatagt tgtaatacct
    15661 ggaatgaaac caatcagtga aggtatagaa atagttggag atatagcaat agttttagca
    15721 ggagcattcc cattagtgtt tgttataaca aaggtattta ataaaccatt aatggcttta
    15781 ggaaaagttc ttggaatgaa tgatgtatca gcagcaggcc ttgttgcaag tttagctaac
    15841 tgtataccaa tgtttggtat gatgaaagat atggacaata gaggaaaaat aataaatgta
    15901 gcattctctg tatcagcagc attcgtattt ggagaccact taggatttac ggcaggattt
    15961 aattctaata tgataacacc aatgatagtt gctaaattag ttggtggtat aacagcagta
    16021 ttgttagcta tggttatcgc aaataagaca ctaaggaagg agaatgtata agatggatat
    16081 atcaaatata gataaaaatt taattgaaac attagtaaga caaataatag aagaaaaaat
    16141 aagtggaact aaagacacag tagattttgt tagaaataaa gacattagtg gaataacttc
    16201 tataaagtta ccaactgtaa aagttagtga atctgataga ttagatacag ggaatccaag
    16261 tgatgtagtt tacacaaaag atttatttac attagaggaa agcccaagat taggttgtgg
    16321 tatgatggaa atgaaagaaa caacttttga ttggactcta aactatgatg aaatagatta
    16381 tgtaatagat ggaactttag atataataat agatggaaga aaggtttctg cttcttcagg
    16441 tgaattaata ttcataccaa aaggaagtaa gatacaattt tctgtacctg attatgctag
    16501 atttatatat gtaacatatc cggcagattg ggcatctcaa aattaataaa aatcaagtta
    16561 agttaataat aatcaaataa ataatttaaa atacaaactc ttaaataaac acacatattt
    16621 aaaaaagcta tgaattaaaa ttcatagctt tttttataaa gaatgttata ttttagacgt
    16681 attttttcta tatttactgg gagacatatt atatttttta ttaaataatc taataaaatg
    16741 ctctacattt ggataaccaa tactatttta ctgataatct tttccttatc actaatctta
    16801 cattttaaaa tattattttt actcgtaata ttatcataag aatttgtttc ttatttaaaa
    16861 ctaattagaa ttataaagtt tattgccatg ttattaataa aataaatcca gttaaatcga
    16921 gtgctagtta aagtaataag aaacttttac aatggtttcc tttatcgatt gaaataaaag
    16981 ttaatatggt ataatatcta aagagagaat cttatcaatt agaatatgca tagaaacaag
    17041 tggcttaaaa ggagacgtta tatggaagag atagtaaaga aggaacaagc aagtatgaat
    17101 gatatttttt tgtatcttaa agctaaatca aactcattat ataaatttgt aatattatat
    17161 aatgagttta tcaataaacc taaagattat ggaactgggg aacttataaa catggttgaa
    17221 gtacatatgc taacagcgat tgaagaaaat ccaggaataa gtccaactca gttagctaaa
    17281 atatggaaca gaactaaagg tgcaatatct caaactatat ctaaattgga gaaaaagggt
    17341 tatataacta aagaaaagaa agaaggaaat gcaaaaacta tattactata tccaaccaca
    17401 tcaggagtag agttaagtaa ggcacataaa ctatatgaca ccatagatgt tacaagaaca
    17461 tttgaatact taatcaagga gtgtacatta gaggagatag atagttttta taaagtaatt
    17521 gatttgttta ttaatttatt agaagaatct ccgcaggaaa ataatgattc tgaaaaagtg
    17581 tagctaagat tgaagttgaa taaagagaaa tagatattat agaagtagta cttaagtaca
    17641 cattatataa aaatataata ggtataggat taaaaagttt agtggtttaa agtttaacca
    17701 ctaaactttt ttaatgtcta taatctacta ttatttaatt tactaaaaat aaaaaaactc
    17761 attatattaa gatgaaaata gaaaaataac tagaaaaata ctataatata tcagcttaat
    17821 acagaaaaaa gttaaaaata ttgagaaaaa taaaaatgaa aacgttttat attgacatag
    17881 agaaaaagta gtgataccat ttaaatatag tttagtagct aaactatatt taaattataa
    17941 taactatttt agggggatgt tatgaattta aaacaattaa gtgaaaaata cgaagaatat
    18001 gtaattcaaa tgagaagaga atttcataag tatccagaat tatcttttaa agaaataaga
    18061 acaacccaaa gaataaggga agaattacaa aaaatagggg tcccttttgt atctattgaa
    18121 ccaaatatcg taatagctac tatacaaggc aaaaatgaag gtaagacaat tgcaattaga
    18181 ggagatatag atgcgcttcc aatgcaagaa gattcagatg ttgaatacaa atcagaatat
    18241 gatggaataa tgcattcttg tggacatgat gctcatggag ctatgttact aggacttgca
    18301 catatgctaa atgatataag aaaagattta aaaggtagag tattattagt ttttcaagca
    18361 gctgaagaag ttggtggcgg acacaaggag ataatacagt actttaagga aaatggagga
    18421 ccagatagat taatagctac tcatgtttgg tccgatattg aagctggtaa aatttctgtt
    18481 gaaccaggac ctcgtatggc tggtgggtct ggttggagaa tagacataac aggtagagga
    18541 ggtcatggtt ctagaccaga tcaaagcata gatcctataa agccagcttg tgatatagtt
    18601 ttaaaagtat cttctatacc agttaatcat ataagtgtac tagaacaatg tgttgtacat
    18661 gcatgtgcaa tacatggagg aactacaata ggaaatgtta ttccaaacaa tgcagaggta
    18721 ataggtggat atagatattt tactgaagaa tcaggcaaaa aagtgtttga cattataaag
    18781 caaatgagta atggtgttgg acaagcttat ggagtggatg tgaaggttac tcatactgga
    18841 ggcataggac ctgttatcaa tgatgaagaa gcagttaaga tggctaaaga aatagtttct
    18901 aatatagatg gattggagtt agattcttat gagccaatat gtgcatcaga ttgttatggt
    18961 gaaatattaa aagagtattc tggtttttat tgttatctag gggtaggtaa caaagaaaaa
    19021 ggaataatat atgcacaaca ccatcctaag tacaatatag atgaagatac attaaagtta
    19081 ggttgcgaat ttatggctaa atatgcagtt gaatttttaa ataaaaataa taactaaaat
    19141 aaatcgaatt ttataaatgg gggggatgaa aatgcaaggt gcattaaagg aaaataagaa
    19201 aaaaagtggt ggaggatttt taggatggat tgaaagagtt gggaataaaa tgccacatcc
    19261 tctagcatta tttacatata ttacattgat agtagtagca ttatctgcaa tagcaagctt
    19321 tttaggtttt tcagcaacaa gtcctgctac aggaaagtta ataaaagtta tgaacttaat
    19381 aagtgcaaaa ggtttagtac tatttatgca agagtttgtt aagaacttcc aaaacttccc
    19441 tgtgcttggg gtagttttag ttatggcagt tgccacagga gtttgtgaaa agacaggatt
    19501 tttttcaact gccataaaaa tgagtttatc aaaagttaag gggaatgcgg ttgtatttat
    19561 aatagctttt ataggagtat ttgctaatca agctggtgat gctgcttttg tattagtacc
    19621 aactcttgca ggagcaatat tttatggatt aaatagaaat ccattagctg gtatattctg
    19681 tggatttgca tcagttggtg gtggatttgc aacagctgta attcctgggg gatgggacgt
    19741 tacgctaacg ccaataactg tatcagctgc tcaaactatt caacctgcat ttgatatgac
    19801 attattagct agttattatg cactatttgt atctgcgttt atagtagcta cagtatcaac
    19861 aatagtaacg gttaaattta tagaacctaa attaggtaag tatactggta aaccagaagg
    19921 tatagatgat aatcaaggtt ttgaggtcac aaaagaacaa gcaaaagcag caaagttagc
    19981 aggttttaca gttttagctt atgtagcgtt attaattgct ttgtgtatac cagctaatag
    20041 ttttttaaga agtccagaag gttctttaat atttggagca ccacttatgt cttgcttaca
    20101 gttttttata gtaatactat tcttcttgcc agggattgtg tatggaatga gggttggtaa
    20161 aatcaagact gtaaaagact taaatgatat attatgtcaa tcaatggcag caatggcacc
    20221 gtttatcgta cttgcaatag ttataggtca attcttaacg ctattctcag tgtctaattt
    20281 agcacaggta ctagctataa aaggtggagc attactcaac tcattgccac taccaccaca
    20341 agttataata ctgctattct tagtactagt agcgtttata aatatatttg ttataagcgg
    20401 aagtacaaaa tggatgatat ttggaccagt atttgttccg atgttaatgc aattaaacat
    20461 acatccagca tttactcagt ttgtatatag attaggagat tctgtaacta atcatttatc
    20521 accattaaat gcattcttta taatactttt agcaacagtc caaaaatacg ataaaaatgc
    20581 aggtatggga actatattct cagctatgat accttacact ataggttact ttgtagtact
    20641 tgcagtttta gtagtagttt ggatgacaat aggtatacca gtaggaatag gtggacaagt
    20701 ttggttataa aacatattta ataattttgg gaggaaagaa aatggaaaca actgaaataa
    20761 gatacaattt tgatgaagta atagatagaa gtggaacaaa ctgtcttaaa tatgatctta
    20821 agagtcaatt tatgccagaa tctccagaag atgcactacc actatgggta gcagacatgg
    20881 attttgcatg tgctcctgag ataattaatg caatgcatga aagaatagac agaaaaatat
    20941 ttggatatag ctctcaacaa actgatgatt attatgaagc tgtatgtgga tggtttaaga
    21001 gaagatttga ttggaaaata gaaaaagaga atatattctt tagcccaggg gttgtgcctg
    21061 cgttaggatt tttcatacaa atattaacac aaaagggtga tggtatcata atagaaaagc
    21121 ctgtatacta tccttttgaa gataaaataa ctaataatga aagaaaagta gttaatagtc
    21181 ctttaaagtt tgaagatggt cattattcta tggattatga tggatttgaa gaattagcta
    21241 aagatgaaaa taataaggtg tttatattat gtagtcctca taatccagtt ggtagagtat
    21301 ggaaaaaaga agaacttaga aaaatagtag acatatgtaa gaaatatgat ttgtggataa
    21361 tatctgatga aatacattgt gatattattc gtaaaagtga aaagcatata ccattagaag
    21421 tagcatgtcc tgattataaa gatagaataa taacttgtac agctccaagt aagagtttta
    21481 acttagctgg tatgcaatta tcaaatatag taatcaataa taaggaatta caagaaaaat
    21541 ggatgcttga aacagattct aaattagcta tattcctacc taatccattc gcaatagttg
    21601 ctactaaagt tgcttataat gattgtgaag attggatgaa tcaagtaaat gagtatatag
    21661 atggtaatat taaatatgta gaagattttg ttaatgagta tatgcctaaa gtaaagataa
    21721 taaaatctca aggaacttac ttaatttggt tagactttag agggtatgga ttagatgcaa
    21781 aacagcttga atatgtaatg cttaacaagg cgaaggtatt gctagatgaa ggatacatat
    21841 tcggagaaga aggagcagga tttgaaagaa taaatgttgc ttcgcctaga acaattatag
    21901 aagagtgtat gaatagaata aaatgtgcat ttgaaggatt ataatagtaa tatctaaata
    21961 atatatttgt gtattttaaa aaaagactat gttaaaaaaa tatttcaaag catagtcttt
    22021 taaagtttta tgatgagttt aaaaaatttc aaaacgttac tattattgat aatcttcact
    22081 tatatgttta tattagaaat aattccacaa tatgctcatt ttctaaagtt gcac
//
LOCUS       DAEPOG010000053        21949 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEPOG010000053 DAEPOG010000000
VERSION     DAEPOG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.053"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            132..1106
                     /locus_tag="KR432_001663"
     CDS             132..1106
                     /locus_tag="KR432_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640203.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1222..2253)
                     /locus_tag="KR432_001664"
     CDS             complement(1222..2253)
                     /locus_tag="KR432_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0640204.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(2865..3716)
                     /locus_tag="KR432_001665"
     CDS             complement(2865..3716)
                     /locus_tag="KR432_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640205.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3733..4653)
                     /locus_tag="KR432_001666"
     CDS             complement(3733..4653)
                     /locus_tag="KR432_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBH0640206.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4763..5677)
                     /gene="murQ"
                     /locus_tag="KR432_001667"
     CDS             complement(4763..5677)
                     /gene="murQ"
                     /locus_tag="KR432_001667"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH0640207.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5776..6873)
                     /locus_tag="KR432_001668"
     CDS             complement(5776..6873)
                     /locus_tag="KR432_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0640208.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(7106..8452)
                     /locus_tag="KR432_001669"
     CDS             complement(7106..8452)
                     /locus_tag="KR432_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0640209.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8541..8849)
                     /locus_tag="KR432_001670"
     CDS             complement(8541..8849)
                     /locus_tag="KR432_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0640210.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9110..9499)
                     /locus_tag="KR432_001671"
     CDS             complement(9110..9499)
                     /locus_tag="KR432_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640211.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(9501..10661)
                     /locus_tag="KR432_001672"
     CDS             complement(9501..10661)
                     /locus_tag="KR432_001672"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH0640212.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11091..12110)
                     /locus_tag="KR432_001673"
     CDS             complement(11091..12110)
                     /locus_tag="KR432_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640213.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDILKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIENSGEIDSYLIDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12110..12784)
                     /locus_tag="KR432_001674"
     CDS             complement(12110..12784)
                     /locus_tag="KR432_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640214.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            complement(12936..14843)
                     /locus_tag="KR432_001675"
     CDS             complement(12936..14843)
                     /locus_tag="KR432_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0640215.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14844..15596)
                     /locus_tag="KR432_001676"
     CDS             complement(14844..15596)
                     /locus_tag="KR432_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640216.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(15914..17068)
                     /locus_tag="KR432_001677"
     CDS             complement(15914..17068)
                     /locus_tag="KR432_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0640217.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            complement(17071..17538)
                     /locus_tag="KR432_001678"
     CDS             complement(17071..17538)
                     /locus_tag="KR432_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0640218.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17552..18040)
                     /locus_tag="KR432_001679"
     CDS             complement(17552..18040)
                     /locus_tag="KR432_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640219.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18054..19385)
                     /locus_tag="KR432_001680"
     CDS             complement(18054..19385)
                     /locus_tag="KR432_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0640220.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19399..20964)
                     /locus_tag="KR432_001681"
     CDS             complement(19399..20964)
                     /locus_tag="KR432_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640221.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21133..21918)
                     /locus_tag="KR432_001682"
     CDS             complement(21133..21918)
                     /locus_tag="KR432_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640222.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
BASE COUNT         7293 a         3809 c         2331 g         8516 t
ORIGIN      
        1 ataaaataaa attatattga ttgtatctaa acaaataggg caataaaata attaagtata
       61 gttacttacc tttcaagcaa tattagtttt atgttagaat tttattatat tgatttacag
      121 gaggtttgca tttgaataat attaattcac ttaaagtaat aaaattatct cttggatgtt
      181 ctcttgctat attcattgct tggctattaa aattagaata ttcaatggtt gctggagtta
      241 tagtactact tacagtaaag gacactaaaa gagaaactct taaaggctcc attggaaaga
      301 tttatggttt tcttttatgt actatttttt catatttatg ttttaatatt ttaggatata
      361 atttgacttc attttcaata tttatattta taattatacc actttgtttt ttgttaaata
      421 ttcaagatgt tattgctatg tgtgtagtca tagcatccca ttactacctt caaggagaaa
      481 cttctatcaa atggatttta aatgaagctg gaatatttgc agtaggaact ggaataggtg
      541 tcttaataaa tatgtatatt ccaacaaata tacataaaat ccatgaagtt caaaaaaagt
      601 tacaggaaga agtgagtatt gtacttatag acattgcaga tattattgta aatcctaaaa
      661 aagaaaatgg atatagtaga gatttatata aattaaattc acttattgac agcagtattt
      721 ctgaaactta cgataatatt aataatactt tattaagtga tactagattc ttcttagaac
      781 atatggatat tataaaaagc caacgggata tcttagaaaa tttgtatagt tatgtatctc
      841 aactaaattc tacaccacca caagctcaca ttctatctgc tttcatacat aaaataggtt
      901 atactgaatt tgaagctgaa acaggcaatc ttcttcttga agaattgaag agattaatgc
      961 ttagtatgaa aaaccatcct ctacctgttg acagaacaga gtttgaaaac agagctattt
     1021 tattcctatg cttgactgag ttaaaacaat ttttagttaa tcgtaaacat gctcagatgc
     1081 ttagagataa taatttttat aaataaaaag gtgagaaatc cagcagttga cctgactaca
     1141 aaatatcaga tataatatta aatgcgcccc tagtaagagg gcgcgatgtt tataacttat
     1201 tctactgtca tgattaaatc attattcata tcgaatatac attatttgac caagtccaat
     1261 attagctttt tcatcagtga tttctatttt aatagtcaca tcattttttt tacagtactt
     1321 tgctaattct gcaccagaac gatttttacc actaaaatct aaagaaaatg gcttatcata
     1381 tttaaaagga tattcaacag gttcaccttc tggtgctaat gcaaacatcc catatggtgt
     1441 gttcataaat cctattacag caattgtaag atttgatggg tctaaatttg atttactacc
     1501 tagaaatgta actgtattca ctgatgaatt aattacaaca catctaacac cttcatacat
     1561 tacatgttta tcctttatat ctccaaattt tccaacaacc tttgccccgt cttgatgtgg
     1621 taacatagtg tgcttcccag gtagtgctgg tttttgcgtt tgtgctactg ctccaactgt
     1681 tggatatgct gtcttatcaa atggtaatgg gtggctaaga ctcttgtata tgatatcctt
     1741 tcctgctgat ttattagcat caatatctac atgatacaaa cgcccatcac caaaacgtat
     1801 aagattccac tggtggtcat ttgatgtgct gtgtacataa taagattcaa ttcccatagc
     1861 gtcacacaaa gctttcatgc tcctagccat tcctgaacat gcaccttttt tagtaaccaa
     1921 tgtataggca gtattcgctc cttctactgc attccagtca acatatgttg tgttattttt
     1981 aatgtgatta taggcaacta aaatcttagc gtctgtagtc atgttctttg taattttatt
     2041 gttcataaaa tctgctgttg cttgtgctac tgagtttagt tgggcttgtg acagctttgg
     2101 accatatacg ctattggttc cagtgacata gctttttggt gtttttgctg ttacctcttt
     2161 ggctgtcata ccataaactg tcatactcat acctgagact aaaacaattg ttgtaatcaa
     2221 taacttggta gctaacttcc taattttctt catacttagt attctccttt ataaaatatt
     2281 gttacttcct attctctacc attaataaag ttcatttggg atttaaaaag gtggattgtc
     2341 aatatgtatg tttcttcatt tatacgttcg tgaaatattt aaatttcatg gaaaatctaa
     2401 tagatatttt ctagataatt tgtatacaat acaactcgca aatcagggtg tttgctgtta
     2461 gtttgagaaa atgatgatga ttaatatatt cggaatcttt gacctataat aaataccatt
     2521 gtcttcaatc attaataatg gtaagagtaa agagaattat caataataca gttaaaatca
     2581 actttgtata catttcatac attctctccc aacaacatta tacttcttat aatttgtata
     2641 ttcaatacgt tttctgggaa aatataggat atatctataa aaatataaaa aaggagttga
     2701 acttaaaatt tgattaaaaa aaatcaattt taagacaact catttgtatt ataaaaatgc
     2761 tatatattat tattaagtaa gtcaatattt tagatattta tctatttctt attgacttac
     2821 gcttatagtc taacttattt gtttagttga tatataacct agttttaagc attattaata
     2881 tttaaataat caagtatcct ttttgtttct tgtagtttct gttctgcctc atttaagttt
     2941 tcttgaatta tagaaatata tattatatct atcaacgtaa gcattgctat tcttgaagaa
     3001 atagctcctt ctctaagcct tttttctata tttggtacta ctagctttaa atcactaatc
     3061 ttactaagat gattgtcact atatttagtt atacaaatga ccttacatcc ttttaatttt
     3121 gcattttcta tagactttat tacttctttt gattctccag agtaagatat tgctatagct
     3181 atatcttctt tatctacatt tgaagatacc ataagttgtg tatgactatc taaagaggta
     3241 aatgcctgtt tatttatcct taaaagtttc atctgtaaat ctagtgcaac taaagccgaa
     3301 ccacctactc caaatatata tacattttta gcctttatta tctgttcaac tgcttctttt
     3361 attatatctt tttcattcaa caaccttgtt tggttgatag tctctatgtt tttgtttgta
     3421 attttgttta taacactatc tatattacaa tcatagtttt catctacata tgtgtattca
     3481 ttttctttgc ccatcaaatc tttagctatc tcaattttta aatccccaaa acccgaatat
     3541 cccatttttc tagaaaacct aactatactt gctgtacttg tttttgagtt tgacgccaat
     3601 tctgatacag aaagcttact tatagcctct gtattattaa gtatgtaatc agctattttt
     3661 ttatctactt ccgttaaatc ttcaaatatc tgttttaatt ttaaaatgca actcatactc
     3721 taccacctct acctatttac tataatattt cttcttagca agtaaacatg ctcctaaatc
     3781 tggagtcaac actggtctca ctataccaaa tctcgtttta ttcaaatatt ttttaagtgg
     3841 ctctaatatt aaatctcctg acttaaacac tccacctgaa taagatacct ttatctttgt
     3901 accttgttca aaatgatgct ctaatcctat tataagccta cttaattcat aagctgcatc
     3961 atcaaatatt ttactagcag tattatcacc ttcaataact gcttttaaac atatcgtagc
     4021 aaactttgct atttctattc tatctccttg tattccatca tttacatatt ttattatgtc
     4081 ataatcattc tctatattaa gatgtctttt tattaaatca tataattttg tctttgaaca
     4141 ccttccatca gactgcattg tgaacatttt taaggtagcc attcctatat aatacgctga
     4201 accttcatct cctaatacat agccccatcc tccacatctg tgaagattcc catctttatc
     4261 taaagcctgt gctattgaac cagtacctgc aattatattt ataccttttt ccccatttaa
     4321 agaacctgct aatgctattt ctacatcatt acctagagta taatttatat gactatatgc
     4381 attttttgtt gctacttcta atgcatacaa tacttctttt atttttccat atccagcaag
     4441 tccaacaaat gcatacgtta tttcttctac atttatctta gcatctttac aaacttcttc
     4501 caacccagtt attaataatt tcgttagatt atcaaatcct atttgattat aatgacaagt
     4561 tcctttggtt atggttccta ctatatttaa ttcttcatct actaatgtaa atgttgtttt
     4621 cgttcctcca ccatctacac ctaaaaaata catactatcc tcctacaata tctttaataa
     4681 ttctttcaat acttaatatc taaaaataac cttccaatgc tgttttttta atattgaaag
     4741 gttatcagta ctattattta ttttacaatt tttctattga ttttaaagct tcacttatat
     4801 aaccttcttg tctatctagg atactctttg cactatcctt atctaaacca ctttttatca
     4861 taaatatagc caatttaact ctattatctg cttcttctaa gaatttttct gcaacgtcat
     4921 attttacatt tgtagctatc attattattc tttttgctct ttcaaccaac ttttcatttg
     4981 atgtcttaac atctaccatc aagtttgagt atacttttcc catcttaacc atagaagctg
     5041 ttgttaacat attacatacc attttttgag ctgtaccact tttcattcta gttgaaccca
     5101 ttattacttc tgcccctact ataatgttta ttggtaaatc cgctttttta ctcatttcac
     5161 tattcggatt catagttatt gatagtactg cacaccccaa ttccttagca tactccattg
     5221 cacctataac ataaggagtc cttcctgatg cagctatacc acatataaca tccttagatg
     5281 taactcctct atctattata tcttgctttc caagttcttt atcatcttca gcaccttcta
     5341 ttgctttaaa tattgcttga tttccaccag ctattatagc ttgaacaagc tcaaaatcca
     5401 ctccgtaagt tggaggacat tctgtggcat ctagtattcc tatcctccca ctggtacctg
     5461 ctcctatata gaataatcta cctccctgct tcattctatc tactattata ttaacagctt
     5521 ttgctatact ctccttttgt tcttcaacag cataggctac agttttatct tcgttattta
     5581 ttaattctat tatgcctaat gtatcttctt tatctatttg caatgtattc ttatttctac
     5641 cttctgtaac taaattttct aaagttttta ttaacatatc atttcctcat tttcttaata
     5701 ttaaattttt aaacacattt taactccaac tttcataatt atatttttat aataaattaa
     5761 tattttcata ataaattatt ttgttgaggg tttaaaatca aacttttgcc atggtttaat
     5821 ataatctaat ataaataatt cttcttttct tatatatcct actacattcg atttaccact
     5881 gtttttcata tcagtcaagg ctatttgtaa ctcccctgca taatgaccat attcactact
     5941 ttcaattact atatctcctc tttttattat tttaggagta ttaaatagtt caaatttgta
     6001 tcctttatat ttaacacgag attgagtaga ccttatcaga ctgtcagata tatcccctct
     6061 attaaaatgt atttcttcaa agagtatctt cttctcaact tctcgtatat tttcctctat
     6121 tgctacatca aatgtaacca tatcttttct catagaacct aataccttta gctcctcata
     6181 tgttggataa caatttgata ttattatatc atttatatta ttcatagcta ttatatgctt
     6241 tacttgaata tctataggta gatttctgtg catctcaatt gttggaagcc catcagttac
     6301 aggccatggt ccaaatgtgg tttcattttg acttgttaca aaaactgctg ttcttaatcc
     6361 atacttatta aatctttctg tacattttat aaagtgttct aaatttaatc cactatatct
     6421 atgtggataa aagttatgac atcctgtcaa attttcttta tttggttggt agtccattat
     6481 tgtatctata taattagtat tattactcat attgatttct attttaagat tttgaggatt
     6541 aaaagtcatt aaggcctttt cagaacctgt aaatcccata tcaagcctta ttccgtctgc
     6601 cttcgtcttt ttaaaaaaag tcaaatcttt gtagcttatt tctaattcac caaatactct
     6661 gggatttaca tcaagtatta cctcaaaacc tttaaactta gcgtattcat ttatactttt
     6721 aaatttttta attatatttt ctttagtatc tttaactgat aataaacatg aaaatatacg
     6781 tgtaaatcca ttttcagcag ctttatctat ataatcatat atttccttag atgttgtttt
     6841 ttctggataa attgatattc ctaatcttcc catctgtgtc atctccttat atcctatatg
     6901 tgtttaaatt tatttcgaat tacacgaaac ttttttaaaa atttggtgaa aaaaagtttc
     6961 atatatattt ataaaaaaag aaaagtttac cttttctttt tttataaaca atcaacaaaa
     7021 tatcttcgtt acttatttca taactgttat actttgaaat ttatactaat ctaatatagt
     7081 ttttaatttc caatatagtt tctaattaat aagcttcttg ctcttcaact ttttcttcat
     7141 gaagcaatct cttatcgtac atcttgacaa atggatagta aattattatt gatatagcta
     7201 tacataataa gttaagaact gaagctctcc aatctccacc agtggctaaa tatgccccta
     7261 ctggtcctgg taatgtccaa ggagcagtaa ctactactct ggatactaaa ttagcactca
     7321 tagcaaacca tgaaactgtt gccattacta ttggtgccac aacaaatggt attattaata
     7381 ttgggtttaa tactattggt gctccaaata ttattggttc atttatattg aatattgctg
     7441 gtattacagt tgcttttcct aaagactttc cataagctga ttttgctcta aatgcaagaa
     7501 gtaaagctaa gcctattgta gctccagaac ctccaatcca tataaaccat tgaaaaaagg
     7561 gttcagcagc aatacctgga actgcttgac ccgcagcata tgaagctgta ttagcatcta
     7621 ataattgtat ccataaaggt cttgccaatg aacctattat tgatacacca tgtattccaa
     7681 atgaccaaaa aaacgttatt aaaaatatta gtataattac tgatggtaat gtgtcagtgg
     7741 ctgaaactaa tggagttact aatttagcta tgaagccatg ccaatcaaac cctaggaaat
     7801 aagtaacaat agccattaaa attattatta atgcggctgg tgtcagagct tcaaaagacc
     7861 ttgtgactga tgctggaact gcttctggca tggttatctt gaatttagat ttgtcagtaa
     7921 atctataaat ctcaacacct attatagatg ttacaatagc aacaaacatt gaacctccac
     7981 caagattagc cattggcatt tgccatcctt gaggaattga attataccaa gctaaaagtt
     8041 ctggactctt ttcaactatc tttaataaat caggggtaag accctctatt gcagtaggaa
     8101 ctattgtcaa aaggaatgta gctgtagcta ataaacctcc tgtcaatcca tctaacttat
     8161 atgactttgc caaactataa cctatgccaa atgtagaata tactgatata ataaaaaatg
     8221 tcattctata tggtagtaat attgtaactg cattttcctt tagatactga gttacccccc
     8281 atgaatctgg taatggagga aatgctaata tcaagaaaaa tgacccaact attataagtg
     8341 gtaatgaagc tacgattcca tctcttacag ctcttaagtg acgttgttct gccaatctgg
     8401 ccatagaagt tgataattta ctgtctaaaa attctgtaaa tttatctagc atcttttctc
     8461 tccttaatta atatttattt ttattatcat taaaattttt ataaaattaa ctttcttcta
     8521 acctaacaac actgcaacta ctatttactc aacaactctt gaacttgttt taataacttt
     8581 ggtcctccta atggactata agcttgtgct tgtattaatg cacatggtac actagcctca
     8641 tcagcaaaag cttttaaata gtcaaatcta tgtctgactt gtggagctac catagcaata
     8701 tcccatccat ttttaacttc attctcaaac tcttgagtac caacagccaa aacatctata
     8761 tctattccat tttttgctcc ttctttttgt aatgcattaa ctgctatagc acttgacatc
     8821 cctgatgaac aaacaaataa tactctcata ttgattcccc ctgttttttt atttcttttc
     8881 aatcattata gcatgtattt ttgattacag gaatacgttt gtatatattt tttcatattt
     8941 tacattttta attgtaattt tatttcatgc atttatatct tgctttaatt gctttattta
     9001 tctaatcatt cttttgtcta aaattacaca ctacttaaaa tactcatatc ttttcttaaa
     9061 aaagaaaaaa gactatcttt tattaacata aaatagtctc aatacttttt taaatatgta
     9121 ttcctttata tatccagaag taacctatta ttgcaaatga taaaacaaat aaaatataca
     9181 gtctaaaaaa tcccaaagaa aaacctctct tagtttcaac aaagtatctt gtatatgcaa
     9241 ctgcaaaaga tgtaattaat gaatttccta taacaactcc aaatctatta tctattccaa
     9301 atatagataa taacaaagga aaaactacag tacttactag agatgaaact attagtatta
     9361 atccactttt aaaacttata gttggaatat tataatctgt tctttgattt ttctccattt
     9421 ctttcttagt atttttatta ctttttgcat ttttataatt attatttttc tttcttttct
     9481 tttttttatt attagacatg tcatttacct ccaaaaggtt taggtgttat attccctaaa
     9541 ataactttat ttttagcccc tgttgcagat attacattag aaaaagaatt atttaatgtt
     9601 tcattgccca agattacaaa tgccaaagcc tcctttgcat cagaagaata cccatattca
     9661 tcttgagtta atacttcaac ctctaataat aactctttta tatatcctat taaagttaga
     9721 ttatgaactc ctccacctcc aattaataaa gtatctacat taacctttgg aagtatgaag
     9781 tttttataat tataatatat agcttttgcc gtaaacatag ttactgttgc tatcaaatca
     9841 tatttatcta catgtttata tttgttaagc atgttatcca aatagacttg tccaaatacc
     9901 tctcttccag tgcttttagg tggcgcttga ctaagatact tatgactcat caaatctttt
     9961 aacatttctt cattaatttt accttttgat gcaaagtatc catttttatc atactttcta
    10021 ttaaataatc tttgacatac tccatctatt atcatatttc ctggtcctgt atcaaatgca
    10081 aatacatcat ctatgttaca tgatttaggt attatagtaa tatttcctat tccaccaata
    10141 ttttgaagtg ctaaattttt atttttatca ctatataata aaaattcact atatggtact
    10201 aatggagcac cttctccacc tgctgctata tccattactc taaaattaga tattaccttt
    10261 gtatttgttt catatgctat aattgctggt tcacctattt gaagcgttga agatacaaaa
    10321 ttattataac ttcttggtat atgaaagatt gtttgaccat gagatgcgat aaaatccaaa
    10381 tttgctatat gaaagtttgc tttatgacac actgatttta cagctttact aaataaatat
    10441 ccaagcttaa aatttaagct acagattaaa tcaacactcg attcctcttc tatacagcac
    10501 tttcttattt cgtcttttat atctttagga atttctagag ttataaattc tatttgctta
    10561 actttagtat ttctaccatt cccactaatt tcagctagta ctgcatcaac tccatctaag
    10621 gaagttcctg acattaaacc tacactatac atagttatca tccttttaag tttatagtta
    10681 ttcctcccca aatcaaggta ttaagtttaa taattttaga attaatctta tttcatcttt
    10741 aattgcgata atttactctt acgaaattaa tcgagttaat cttatacacg ttttttacct
    10801 tagctaaatc ttacattaat ttattttcag tgtcaatgta atgcatgtaa ttttttttca
    10861 caaacaaaac atattctctc tccaaaaacc atattaaaat agttactaca aataaaaaac
    10921 ataccaggta cctatctgat atgtttttca tatataataa ttattatttt ataatataga
    10981 tagaacatca tttaaggcac taattaaatc ttcactattt tcaagaacta aatttattct
    11041 tattgtatat gtcgatttgt atatgatgat agattttata gtctaataac ctaactatct
    11101 aggcttaaat aatccgatat atcataaaag tagatattaa aaattgtata tgaaccaatt
    11161 ttcgaacata cctcaatctc atgccctaaa ctatctatca ttttctttac aaaatacatc
    11221 cccattccag tagacttata caattgattt cttccatttt tccccgtaaa tcccttgtca
    11281 aaaaccctag atatatcttc ctcatttata cctataccaa aatcttttat agataatacc
    11341 actccatttt ctaacttttt tgaattaaat tcaatcttac caacttcctt tgaatacttt
    11401 atagcattat taataatttg gtctaaaaca tatgacatgc atttagaatc tgtgtaaaca
    11461 tcattttcta attcattcaa actaacttct acattatttt ttataagcaa aaatgaatta
    11521 ttttttatag acatttttac caatttttct aaattaaatt tatttataaa tacatcttca
    11581 aacatagtgt tgcttctact actatacaaa atagagttta ctaaaaaatt tatctttgca
    11641 acctgattct taatatccaa actatcttct atgctactta acctatctgt aattatactt
    11701 aaagaagata ttggaatttt tatttcatga acccatttag ctatgtaatc atctaattct
    11761 tttaatgatt gtttataagt ctctatgtca ctattatttt ttatttttaa gttttctata
    11821 atatctttta ttagattttc ttcaaaagat tgtccatcaa ttaaataact gtctatttca
    11881 ccactatttt ctatacaatt atataaattt atataactat ttctaaaatt tatataatct
    11941 ataacaaaaa ataccattat tataaacagc actaaaaaat ctaagtatat taattctgaa
    12001 tatctatttt ttaaaatatc tatagcaaat aaatagatat ttattatact aactacaact
    12061 aaaggtagag caatatactt agacctacct tttaaatatt ttactagcat tatattatat
    12121 atcctatccc cttcttagtt tttataaaat catcaaaacc tagttcttta actttacttc
    12181 tcaatctatt catatttaca gtaagtgcat tgtctgtcac aaactcatca ttatcccata
    12241 gagtcatcat aagactttcc cttgatacaa ccctattctt attttttaat aataatgtta
    12301 aaatttttat ttcgttttta gtcaactcta tacttttatc cttatactta tgttttataa
    12361 tccctttccc cacatcaaac atcaaatctt cacaatacaa aatactatta gaatcactat
    12421 aattatagac tcttcttatt ataccattta ttttggtaac caatatgtct atagaaaatg
    12481 gtttggtaat ataatcatct gctccattat ttatacccat tattaaatcc atatcagaat
    12541 ctcttgatga taaaaatatt aatggtacat ttgaaatagc tcttattttt tcgcaccagt
    12601 aaaatccatt atataatggt aaatttacat ctaaaagtac tacatctggt tttttatcca
    12661 taaattcatc taatactacc tcaaaatcat caattaattc tactttaaat ccccattttt
    12721 caagtgctaa cgcaatctct tttgataaat caatatcgtc ctctactaat aaaattctat
    12781 acatttgtat attctccatt atcctaatat ttcactatgc catgaattat taatatcata
    12841 acatatttta tataaattta ttacccaaat tatttagtta atcaacaagg agactcttat
    12901 tacaagaatc tcctttctct ttattaatat atcaactaat ttttatacac tacactctta
    12961 aacttattaa cagatactgc tccatatatt acgaatatta tcaaacatat cactaaactt
    13021 agtatatata taccagataa atcgttcatc aataaatctc ctgctaatct caatgcaaaa
    13081 taactagata tagttgcact taacattggc aaagtataaa atatagctac ttctttaagt
    13141 atagctctat ttatatcttt ttctgtagta cctattttta ataaaatctt gtatttctct
    13201 ttatcttcat atgcatcact atatattttc atatatatta tacttccagc tacaattgca
    13261 aagactaaga ataaaaacac aagcactaca tatgcaaact tcatccattg actctcacct
    13321 tgagctttat atccattctg aatatatcca ttttttaaac ttaaattctt ttcaatattg
    13381 ttgaacatta cttggctatt ttcttcttga aatacctttg caccataaaa atatacttgt
    13441 tgacccttat ctcttagttt aaaatatgta tcatcattta ccactataat actttgatta
    13501 agtcctgtcc ctaatactgg aacttttact tctccccttg aaatattata taactgacca
    13561 tcaagttttg tcttgacttc ttttgtagct attgatgcta tcgtttgagg actttttagc
    13621 tctattacat tatttccact aactaatctt tcaccatact cttttaattc tttatcattg
    13681 ccattcactt taagagtatt acaaaaatca ctatatttca ttatagtatc tgttgtatca
    13741 taagcttctt ttatatctgt tttctttaag attgttagtt ctggatttac ggagtacttt
    13801 atcttattag tatcttttat tatgttctct atttgttttt tattcacttc atctttatcc
    13861 attgaaacta tgctaatagt gtacacattt ctttgttctt gggcattctt atgtatatgc
    13921 ttcatagcaa ctgaagcacc aagagttgat attgttgtag ttataattat agctattata
    13981 gcataagttt tatagttttt atttaatcgg tagtttatat tgtttatcgc aattatatta
    14041 tttccattaa ataatatttt tttatttttg attataatat caaatataac aggcataact
    14101 gatttgaata agccataagt acctactatt attataccta taacaggtag tacactagtt
    14161 atatctccag ttcttatatt cgtagaaaga tagtatccat atcctattac tataagtgat
    14221 actatagcta atatatatat acctattcct acctttggta ttttttcttg ttttttagat
    14281 atattgatta gatttattac actacttcta catattgtta taaaaccttt tacagccatt
    14341 attataaata tagaaataaa gattaaagta gcatatagta tagcttctat gctaagtcca
    14401 ttagatacct ttatattaaa tccagataat tttattatca ctacttgaaa aaatctagat
    14461 gttattatcc ctataattat tcctgttata caagaactta ttcctataag catattttct
    14521 acaaaatata tttttcctat agtatataaa tctaatccca taaaggacaa tattccaatt
    14581 tctttagacc tattcttaaa aaatatattt gttgcatacc atataaagaa aaataaaaat
    14641 actgatagta tcattattac agctactaac actgatagtg taaatttttt attttcctct
    14701 tgtaacacat ttatagcatc tccatatctt aataattcaa aattagtcag cacaactact
    14761 gaaaaaacca tagatataaa aaatgctaga taagttctta tgttactctt aaaattatta
    14821 taagcaattc caagtatact cacttacatc tcacctccca tagaggctag catatccata
    14881 atttttccat aaaattcttt tctactattt cctttgttta atttacactt tatatttcca
    14941 tcatttatca tatatacttc atttgaataa cttgccgtta gagggtcatg tgtaatcatt
    15001 ataatagtag cgttgttttc tctgtttatt ttttctaatg attctaaaag ttcatatgct
    15061 gatttagagt ccaaagcacc tgttggttca tcagcaaata tgactgctgg atttgtaatt
    15121 aatgacctag ctgctgcgcc tctttgtttt tgtcctcctg ataactgata tggatattta
    15181 tctaaatgat tttctaatcc aaatttttta gctatagaaa ataccttagc ttctatctcc
    15241 ttgcttttct tttttcctaa tgctagagga agtgctatat tgtccattag tttcatattg
    15301 tctaatagat tatagtcttg aaatataaac cctatttttt ctcttcttaa ttttgatagt
    15361 tcattatttt ttacttttga tatattaatt ccatctagta ttatttctcc tgaagtttgt
    15421 ttgtcaagtg tagacattaa atttagcaat gttgtctttc ctgcaccaga tggacccatt
    15481 attgctataa aatcaccttc attgactgta agagatatat tttttaacac atttgtttta
    15541 aatcctttta ttccataatc ttttgatata ttttttattt ttaatatttc tttcataata
    15601 atagcctcca gttgctaatt ttttaatata tatttatttt aaaggtttta tataaataaa
    15661 tccttaagta aatattacat tttaactgtt agcattacac ttttgtaaca tatggaagac
    15721 tattataatt atagtggaaa tatcttaaaa taaaagcatg attcactttt ttgtataaac
    15781 aaagaaaaga agcccctaag aagcctctaa tctatacaat aattcttctc tataaaaaac
    15841 caaaaataca accttgattg tggcaaatac aggtactata aacaagtgat attactacta
    15901 tatattaaaa caattattct tgaagataat tcaataagac tatcaagcca taagctaagc
    15961 atccaaaaag acttaatatc attaatgtat aattataccc aatattaata ctcaaaaaac
    16021 cagcaacagt tggaataatc agagatgaac ctcccataaa tatacttaaa gctattaatt
    16081 ggctactact tctaagtttt ttaggagaaa cttcaaatat taatcttttt gaagtaatta
    16141 aaattaaagg agtagaaaac acttgaaaaa cacacagtat aataatgata tttgaatcat
    16201 ttgttaaagc aaataataga aactgaactg ttatcataat agcacttaat tttaataagt
    16261 ttaaactgct atattttttt aataaataag aaccagccag atatgttggt atctctaata
    16321 atgactgtaa agaccacttt aaagatatct gagaatttgt agcatttaaa gctatcattt
    16381 tatctattac agtacagtta tttgctacca ccattgagta taataagaat aaaatacata
    16441 ctagtaagat atatttttta tcttttaata attcaataat atctttatta gttacattta
    16501 ttttcttatt aatcttttca atatcaggta ataatagtat atttataata agtaatacta
    16561 caattaaacc aatcgctaca gataatccaa aatatgagaa tctaagaatc aagtaagatg
    16621 ctaccatact tccaatagcc catccaatcg acccaaaagt cttaataaat gacaaataat
    16681 tattaatatt atctccacaa cctattatcc atgtatcata taaactacag caagtattta
    16741 ataatcctcc tgataaagca acaagtaata agtatattat aaaagttgca gattcagtag
    16801 aaaacataat aactgatgca attccataaa ctgataaact aattaaaaca atttttttca
    16861 ttgtttgata cttatcagct aatactccaa ataataattg aaataatata ttggttattg
    16921 catagctcga taatataatt cctctttcaa acgctgagta acctaatttt gataagaaag
    16981 gtgtgaattt tgttgaagca aaggcaaaaa taatatatga gaaaaaatag aatatgataa
    17041 atttaaattt aatcgcttta ttaatcataa tcaatcaaaa ttgataactt taataccact
    17101 atttccttcc tgatttaagt ttgaaagatg atattttacc gtattaggat tctctacaat
    17161 aactaaagga atatataaag tattttcaag taacttatta tcaatttcta ctaacttttc
    17221 ccccatatta acttcttgac cttgtttaac ttctaattta aagttttctt tttttgttgt
    17281 tgcactatcc aatccaatat gaataagaat ttgaacatca ttttgcaatt taattcctat
    17341 agcatggtta gtttgagcaa taaaacttat tactccttta gtcggtgatt taagaatatt
    17401 gtcctttgct atcatagcaa atccatctcc cattacctta gttgaaaata ctttatcagg
    17461 aatttctcca agattaatta tttttccatc aatgtatgaa ttaattgtac ttgcttcttt
    17521 ttttctttta aagaacatta tagacactcc tctacataat taactttatt ctaattatca
    17581 ttacaaataa cgatattaag catactgcaa ttgaggaaaa aagagcctta tagctctttc
    17641 cccattgtag ttctttaata ctgcttatga tttgtttaat tccaacaaat ttataaatat
    17701 aggtaaacat gataacaata gttattgtgt aaatttggaa tatctgccct aataagacta
    17761 aatagaaagc aatcatgttt tcttttatct ctttattctt ttttatattg taaataacat
    17821 atgcactgta tactgcacta aagttagaga ttaaatttaa acttgcatta acatcattgt
    17881 ttaaaagttg tgaaaatgaa atgtttgtaa ataatgatgt caattctaaa tacactatat
    17941 ataaaacagg taatattaga aaacaaacca taaccactga aagaaatata ttttcatctt
    18001 ttaatactgc tgttatattt tttattttat ttgttatcat gtttttgttc tccttattta
    18061 aaagttggcc agaaatcctt attagccaca atcatttcat ctaagatttc ttttgctact
    18121 tttgcacttg gaacagtttt actcatagta aacgactgcc ataatttttg ataacttttt
    18181 tctatccatg cttctacagc taatttttca gaagcaactt gttgttccat taaacctttt
    18241 tggaaagttg gaattttacc tatagacata ggttcaacca tatcttttcc aacaaggcaa
    18301 ggaatttcaa tcattgcatc atctgcaaaa ttttcaatag ctccattatt aggaacaatc
    18361 agtaaaaatc tttcctgagt attaaatgct aatgcacaag ctaattcaac aataaattga
    18421 gaatgttcac ttgcatcaaa ccatgtatcc cttgctgtgc cattttttat aattctttca
    18481 cattcttcga atgtatcctt ttctcttcta tccataattt catttgctct tgtataattt
    18541 ggattagcat gttcaacagt ttcatcagcc attaaataat attttaaata agtattaggt
    18601 aatgtagtcg gttctatttc taataagtcc tttgctgctc tcatagtttc taaccaacta
    18661 gattctttgt gttgaccttg aggtgtaaat tcctcatatc cttttttagc acaatattcc
    18721 tgtaatttag gcattaaatc atttccttct ttgtccttaa tacttgtcca ccacccgaaa
    18781 tgattcaatc cataatatct aacatcaaag tctttacgag aatttaaacc taaaattcta
    18841 gcaaatattt cctctaaaca tactggcata tcacaaatat taataattct tgaatttggt
    18901 cttaatacac gacatgcttc tgcaactata gctgctggat ttgaataatt aagcatccaa
    18961 gcatttggag aatatttttc catataatca atcatttcga ttattggacc tattgttctt
    19021 aatccatagg caattccacc tgccccacaa gtttcttgac caacaacccc atattttaat
    19081 ggaatttttt catcttgttc acgtaaagca tataaaccaa cacgaatttg tgccattaca
    19141 aaatcaatat cagtaaatgc ttcttctgga tttgttgtat aactaaattt aatttctgga
    19201 tttttctctc taacaattat tgcacatgct tcagcaactt ttctttgtct ctcatcatta
    19261 ttatcatata atttaattga ttcaatagga aatttagcaa cactttcaat taacattgtt
    19321 acaattccag cagtataagt actaccccca ccagcaatta gtatcctctg tctttttctt
    19381 tccatctttc cttctccttt ataataaatt ttcaaattct tctcttacac ttggtactga
    19441 aagcccaata ataatctgaa tagcttttcc attatgcact aaaccatgtg cacctactac
    19501 cttaaaatct tcttctttag aaataattga gtcatccttc aaggttaatc tcaacctcgt
    19561 agcacagttt gtaacatcta caacattttc tgctccacca actaatttta tgtattcaga
    19621 tgcttgttga gaggctttac tcaatttatt tcctttttca tctttagttt ctaagtattc
    19681 atttttagta tatagttttg tttcttcatt atcttcctct ctaccaggag ttttcatatc
    19741 aaacttctta attaagaaag taaataaaaa gaaccagata aatgaaaata taatccctac
    19801 aacaacttga acaacatata ccttccagtg atacattcca agaggaagcc aatttaatac
    19861 aacccagtta ataattcctc caccaaaatc accaacaact cctaaggcaa acgaaatcgt
    19921 ttgcaagcat gcatctaaaa atgcatgtac taggaataaa agtggtgcta caaataaaaa
    19981 tgtaaattct aacggctcag taattccagt taatactgca gtaagagtag caggaatcat
    20041 taaacctaat acttttttct ttttttctgg ctttgcagtt acatagaaag caccaaatac
    20101 acctaatgaa ccaaacacct tcgataatcc agataatgaa aatccgattg gatacatttc
    20161 ctttagagtc ttagctgaag tttgaaagtc ctgaatatga gtagcccaat atactgatgt
    20221 accacctgga acaacaacac tatcataaca aattggtgca tatacaaaat gatgtaatcc
    20281 agttggtatt aaaatctttt gtaagaatgc ataacaccaa actcctatat ttccactatt
    20341 tataaaaaac gtttgcaaat gtctaataac attttgaata tgaggccaaa taaaacaagt
    20401 taataatgca actggtatca taacaaagaa acaaattgca actacaagtg atgagcctct
    20461 aaataatcca ataaaatctg gtagttcttt atcataaaat ctattatgaa tataaatgac
    20521 aatacctgat attaacaaag caccaaccat accagtatct aatgttttaa taccagcaat
    20581 gcttacaagt ccacttccag cacttatttc agctgcgaaa tcaactccaa agctttttcc
    20641 ccaaaaactt aacatagaac ctacaaaata attaaaagtc aaatacgttg ctaaagcctc
    20701 catacaagct cttgcttgtt gcttttttgc aagtgctatt ggtaaagaaa tagcaaataa
    20761 taatggtaat tgattaaata ccatccaagc tccactagat attacacccc aaaattggta
    20821 ccaaatattt tctggtgtag caagtgaacc cataattgac tgattttgaa atacaattga
    20881 gaatccaacc attatcccgg caaaagcaaa taatagtgtt ggtgtaaaca ttgcccctcc
    20941 aaaacgctgt attttcttca tcatataaat tactcccctc ctcatatttt tagtttatct
    21001 ttggccttag atatctatta ttttaacagg ttaataaata tatacaatct ttagggtaca
    21061 tttggagctt attcacagtc aaataagttt ttgcacattt ttatgttcaa attctcataa
    21121 aaaaaagaat gactacaatt tgcaatcaat cccttctgta atcattctat gccttttata
    21181 aatactatat ttaataaata ataactcaat caatataaaa tataacattg tcaatttata
    21241 actagggtgc atttgtaaaa atgagatatt agtcgctgaa atataaattg attcatcact
    21301 taaatcagtc aatgtattat ttttaaactc tgtaattgaa attattttag ttcctattaa
    21361 ttttaatctt ctagcaatat taagtgtatt ctcattctca ccacttaggg atataataat
    21421 cataacatca tttttactga caatattgta aaaagaatca tcaaatgttc ttccctcaaa
    21481 atcatatatt atttcttgac atgataagaa cattctcttt atctgttttg caaccacatt
    21541 ttgaatatca cctgaaccat aagcgtatat tgttcttgat tcataaagaa gtttacacaa
    21601 tgcatcataa tctttatttt tttgatcttc aacatactta attattgaat tacatgcaat
    21661 atcaaatgac tgattttcta tgacatgaac cttttctgat tcccagctta ataatacttt
    21721 aaactcactg aaacctttta aatctaattt tttaacaaat cttgtaatag ttgcacgaga
    21781 cacattacat ttcatagcaa gttcatttat tgacatctta ctacaatgtt ttttatttgc
    21841 agttatatat ttccatatta ataaatctgt atcattcaat ttattataat ttaaaaaaat
    21901 catttcttct aatctcataa actattatcc cctttaatta aaaatcgtc
//
LOCUS       DAEPOG010000054        21930 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEPOG010000054 DAEPOG010000000
VERSION     DAEPOG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21930
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.054"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="KR432_001683"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="KR432_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH0640223.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="KR432_001684"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="KR432_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH0640224.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="KR432_001685"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="KR432_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH0640225.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="KR432_001686"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="KR432_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH0640226.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="KR432_001687"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="KR432_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH0640227.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="KR432_001688"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="KR432_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH0640228.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="KR432_001689"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="KR432_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH0640229.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="KR432_001690"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="KR432_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH0640230.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="KR432_001691"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="KR432_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH0640231.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="KR432_001692"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="KR432_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH0640232.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR432_001693"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR432_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH0640233.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="KR432_001694"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="KR432_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH0640234.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="KR432_001695"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="KR432_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH0640235.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="KR432_001696"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="KR432_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH0640236.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="KR432_001697"
     CDS             6977..7162
                     /locus_tag="KR432_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBH0640237.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="KR432_001698"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="KR432_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH0640238.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="KR432_001699"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="KR432_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH0640239.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="KR432_001700"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="KR432_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH0640240.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="KR432_001701"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="KR432_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH0640241.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="KR432_001702"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="KR432_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH0640242.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="KR432_001703"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="KR432_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH0640243.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="KR432_001704"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="KR432_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH0640244.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="KR432_001705"
     CDS             11105..11755
                     /locus_tag="KR432_001705"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH0640245.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="KR432_001706"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="KR432_001706"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH0640246.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="KR432_001707"
     CDS             12524..12793
                     /locus_tag="KR432_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBH0640247.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="KR432_001708"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="KR432_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH0640248.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR432_001709"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR432_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH0640249.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="KR432_001710"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="KR432_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH0640250.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="KR432_001711"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="KR432_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH0640251.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="KR432_001712"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="KR432_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH0640252.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="KR432_001713"
     CDS             14889..15836
                     /locus_tag="KR432_001713"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH0640253.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="KR432_001714"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="KR432_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH0640254.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="KR432_001715"
     CDS             16318..17151
                     /locus_tag="KR432_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0640255.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="KR432_001716"
     CDS             17139..18005
                     /locus_tag="KR432_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0640256.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="KR432_001717"
     CDS             17999..18802
                     /locus_tag="KR432_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH0640257.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="KR432_001718"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="KR432_001718"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0640258.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="KR432_001719"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="KR432_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH0640259.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="KR432_001720"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="KR432_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH0640260.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            20996..21748
                     /gene="cwlD"
                     /locus_tag="KR432_001721"
     CDS             20996..21748
                     /gene="cwlD"
                     /locus_tag="KR432_001721"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBH0640261.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8807 a         2558 c         4501 g         6064 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgtaattta
    20761 aaagtttata taaggttata tattttaaga tattatttaa caatataaat tctgaatata
    20821 cgttatcaaa gtttataata taaactaagt ataatttatt gtatagtatt gaatgttaga
    20881 taaacatagt gaattaaata ggggcagaaa tataatatca ttacataata atatattaat
    20941 accaataaat aaagatatat caatattaaa aaaatttaaa tgtttgtact aatatatgga
    21001 atattatagt gtttttataa gtatacatat acagggtgat gttgtgagaa agtacataaa
    21061 acatataatt tttagttttg caatgatatg tctagtagta gtatccatat ttgaaattaa
    21121 aaatatttct gaagatgtta tcaagtatat gccagtaaca aacaaaacta taattttaga
    21181 tgctggtcat ggaggcattg atccaggtgc attaaataag gataagagta catctgaaaa
    21241 ggatattaac ctagcaataa cacttaagct tagagagctt atagaatcaa gtggtggcct
    21301 tgtaatatta actcgagaag atgacagtag cctttataaa gaagaaaata ataaaactac
    21361 aagacaaaaa tataatgaga atttgaaaaa tagaaaagaa ataatatcta attctaatgc
    21421 caatatgttt gtctctatac atcttaatgc atttgaacaa tcaaaatact atggagctca
    21481 aactttttat cctaaagaca aacaagacag taaagaatta tcgaagtgta ttcaagaaga
    21541 acttaaaaga gtagtagata agacaaacaa cagagaagtc aaaccaaggg atgatatata
    21601 tcttttgaaa gaaaataata ttccatcagt actgatagaa tgtggttttt tatcaaatga
    21661 aaaagagtgt aaactcttaa ctgatgaaac atatcaagaa aaaatagcat gggcaatcta
    21721 cataggaata caaaaatatt taagttgaaa taagtataaa aataggtcta aatatgtaaa
    21781 tagcttgtat taaagcagtt aaaatgcatt aatataggct atttttattt tgacaaaaaa
    21841 atatttaaaa taaaagttaa aaagttgttg acttagaata atatagatga tattatatat
    21901 gagtgcccaa aaggagcacc aaaataagac
//
LOCUS       DAEPOG010000055        21841 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEPOG010000055 DAEPOG010000000
VERSION     DAEPOG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21841
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.055"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            597..3500
                     /locus_tag="KR432_001722"
     CDS             597..3500
                     /locus_tag="KR432_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640262.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3533..3979
                     /locus_tag="KR432_001723"
     CDS             3533..3979
                     /locus_tag="KR432_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0640263.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4102..4404
                     /locus_tag="KR432_001724"
     CDS             4102..4404
                     /locus_tag="KR432_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0640264.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4585..5991
                     /locus_tag="KR432_001725"
     CDS             4585..5991
                     /locus_tag="KR432_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0640265.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6335..7393
                     /locus_tag="KR432_001726"
     CDS             6335..7393
                     /locus_tag="KR432_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0640266.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7468..8154
                     /gene="araD"
                     /locus_tag="KR432_001727"
     CDS             7468..8154
                     /gene="araD"
                     /locus_tag="KR432_001727"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH0640267.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8172..8819
                     /locus_tag="KR432_001728"
     CDS             8172..8819
                     /locus_tag="KR432_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0640268.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(8992..10359)
                     /locus_tag="KR432_001729"
     CDS             complement(8992..10359)
                     /locus_tag="KR432_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBH0640269.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10549..10637)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11065..13185
                     /locus_tag="KR432_001730"
     CDS             11065..13185
                     /locus_tag="KR432_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH0640270.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13289..14542
                     /locus_tag="KR432_001731"
     CDS             13289..14542
                     /locus_tag="KR432_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBH0640271.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14696..15757)
                     /gene="ytvI"
                     /locus_tag="KR432_001732"
     CDS             complement(14696..15757)
                     /gene="ytvI"
                     /locus_tag="KR432_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0640272.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            16031..16771
                     /locus_tag="KR432_001733"
     CDS             16031..16771
                     /locus_tag="KR432_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBH0640273.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16783..18561
                     /locus_tag="KR432_001734"
     CDS             16783..18561
                     /locus_tag="KR432_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0640274.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18728..19645
                     /gene="pfkB"
                     /locus_tag="KR432_001735"
     CDS             18728..19645
                     /gene="pfkB"
                     /locus_tag="KR432_001735"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0640275.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19682..21601
                     /locus_tag="KR432_001736"
     CDS             19682..21601
                     /locus_tag="KR432_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0640276.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         8764 a         2344 c         3796 g         6937 t
ORIGIN      
        1 gttcctactg cttttttctc attaaaatta ggaaaattta gtcttgcaga ttttgtaaat
       61 atattaaata tgtcttttgc agaatatttt aataaaactt cagttttaaa tgtattcata
      121 tcgttttccc ttctgtatat aaatttaata tttataagtt aatttttaag ttaaaatagt
      181 ataatatgcc atattaaatc atgttaaaac tatttatata gctaaaacct tacctatata
      241 agtatgtata ctatcatata aaaataaaat cttaaaatag aaagggttaa aaacacagtt
      301 tattataatt tttattgttg agtagatacg tactattata tatgattgag caagtttttc
      361 aatcataaat ttataaagta ttttgcatta aaatttaaac aaagagttaa attatttaag
      421 attatgaata actaatacat ttagtaaatt actaaatata caaaatgtaa atacttatca
      481 aaatgtataa atacttatga catctagtta cttacagggt ttaagtagaa aaaaatatca
      541 aagtaagtat ggcatgatag ttgcttatat atttatcaag aaaggagtta ttaagcatgg
      601 agttgagaga taaacttgta aacatcataa atacagaaaa taaaaaagat ccattaacag
      661 atgttcaaat agcaaagttt ttaagtacaa ctagagagaa cataactaat ttgagaaaag
      721 aacttaatat aagtaattca aggcaaagaa gatatcctta tttaaaatca gcaatttcgg
      781 caatattaca aaagaataag aatatttcta tatcagaaat tacaagggaa ttgatgacag
      841 aaggctttaa tatatcgaga agagttgtgg aggaactttt acctaaagaa agtttagaaa
      901 tggatgtagt agaggaatct gaagaagaat cgaatgaccc atttgaaact ctgataggta
      961 ataaagggag tcttagaaat gctgtggagc aggctaaatc tgctatattg tatccaccaa
     1021 aaggattacc aacgttgatt attggggaaa gtggagttgg taagagttta ttttctagac
     1081 atatgtatga gtttgcaaag caaaaaaaga tagtaaaaga aagctccaat tttgttgtat
     1141 ttaactgtgc tgattatagt gacaatccac aattattatt atctttgtta tttggatata
     1201 aaaaaggtgc atttacaggt gctgaatatg atacaccagg tcttgtagaa gaagctgatg
     1261 aaggagtttt atttttggat gaaatacata gattgcctcc aaaaggtcaa gaaatactat
     1321 tttctatctt agatagggga aagtttagga gattaggtga aactaatgct gaaagaaaag
     1381 taagtattat gttgattggg gcaactactg aaaatgtaga gactaattta ttattaacat
     1441 tcagaagaag gataccaatg ctaataactt tgccaccatt acatgataga ttactaaaag
     1501 agaaaataga ccttatatac aatatctttc aacaagagtg taatagaata aatgcaaaaa
     1561 tatttgtaga taaaaatgtg attgaaatac ttgtactaaa aaagtttagt ggaaatatag
     1621 gacaattaca aaatatgatt caggttcttt gtgcaagagc ttttatgaag tttataaatt
     1681 ccaaagatga agatagtgaa tcgatagtag tagtagatat aaatgaagtt ttaaagctta
     1741 aagattcttt taaagatgct gcctttcaag agattgaata tacagagata agaaaatatt
     1801 tgaaaaatgc tatatttata ccatttaatt tagaagaatc atcagtaaaa ggaagtcttt
     1861 taagtgatga atatatatta ccagaggaca tatacaaaac aattgaaaag aaatattatg
     1921 acctaaaatc cctagacatt gaagatatag atatagagaa tattttatgg acttttattt
     1981 taaatagatt tgctgactta aagtttaatc tcgatagtaa tgaagaaatt ttttcaatga
     2041 gtgatttaaa tagttttgta agtgatagtt tagttaaact ggtaaaagaa cttatgaatg
     2101 atatagttga aaaaaatatg aatttagaag taaataaaaa tatgttcaaa tatctagcaa
     2161 tacacttaga agaagctata aaaagaatta agctaaatca aaagataata aatgtaaatt
     2221 tagaaaaaat aaagattgac ttttcaaaag aatatgaaat tagtaaaagt tttgcaataa
     2281 aaattgagga atcaattagt attaagattc cagatgatga aattggattt ataacactct
     2341 acataaagtc tgctctaaaa aatgaagtga aaaaagataa agtagggtta ataataattt
     2401 ctcatggacg tatagctaca gagactgtaa atgtagttaa agaactttta ggtgtaaagt
     2461 ttcctgtggc tatagatatg cctttagatg aaaaaccaat aaatatatac aataaggcag
     2521 tagaactgtc aaaaataata gaccaaggga aaggtatatt gtttttggtg gatattggtt
     2581 cacttacaaa cataggtcaa atagtaaata agagaaccaa tataagcact aaaactattg
     2641 atagagtaga cctcctaatg gcactagaag caactagaaa agtttctatg ggagaggaag
     2701 aacttgatga aatatttttt tctttgctaa aagataggat gggatataac tatgaattag
     2761 aaaaacacat agataaatca aatgcaattg taacattatg tcttactgga gaaggaactg
     2821 ctaagtatat aagtaaaacg ttggaagaaa agtatgataa cactaaatgt tatcaaatga
     2881 gtgcattgga tgaaaattta ttttgtaaaa ttgaaaatct aaaagagaca aacaatatat
     2941 tagctattgt tgggacaata aaccctaaga taccaggtat aaattttata ccctataata
     3001 aagaactttt caaaaatttg gatatctatc tatcaaaatc aaaacaacaa gatgacagac
     3061 cagttaaatc gtatgaaaga atgcttgatg aggatttagt gatttttgaa ccagatatat
     3121 attttaaaaa ggatttatta gaatatgtat gttcaattct tataaataaa gaatatgtag
     3181 aaaaagatta cttagattct gttttacata gagaagaaat gcttccaaca tattctaaag
     3241 gagctatagg tgtgcctcat ggagattctt ctactgtaaa tagtacaagg tttgtttttg
     3301 ttaaactaaa gaatcctatt gattggggtg taggtaatgt aaatttcata tttatgccag
     3361 tttttcaggc taatgataaa gagattgtta aaaatgttct tgagatacta aaagatgcag
     3421 aatttatgaa caatgccaat aagtgttttg ataaagatgc ttttagaagg attatatttg
     3481 ataaatttaa acatctgtag ccatagcaaa aaaattgaga ggagttagag taatgaatgt
     3541 agatttaata tcattgggtt tagaatctaa tgattatgaa agtgttattg aagaattagg
     3601 aagtataatg tgtaaaaaag aatatgtaaa agaaacatac ataaatgctg tcttggaaag
     3661 agaaaggaca ttaccaacag gtctagatat tggagaaatg tgtgttgcta ttccacatac
     3721 agattcaaag catgtaaatg agtctaatgt agctgtaggg atattgaaaa atcctgtaaa
     3781 gtttaattca atgatagacc caaaagatag acttgatgta gaacttgtat ttttattagc
     3841 agtaaagaac ccagattcac aagtaaaact gttaaaagat ttaatgtcag tttttcaaaa
     3901 tataaagtta ttaaaaaata taaaaaacgc atcaacaaaa gaagaagtag ctaaattatt
     3961 ggattttata gaaatttaat gtaggagaat tttataaata aaaaattaaa atttcaacaa
     4021 tcaaagtaag tattattttt ttaataggtt taatcttata taaaataaaa atttagagtg
     4081 aaaaattagg aggaaattat tatgaaacaa tttaatgtat tatcagtttg tggttcaggg
     4141 acagttacat catctatggt agcaggtaaa ttaaaagaag tactaggtaa taaggggatt
     4201 tctattacta caacagaagc tagaccaaca gaagcactaa atttagcaca atcaggaaga
     4261 tttgacttta taacatttac gagtccgctt caaacaggcg attatggaat accaactata
     4321 aatgcatttg cttgcctaac tggtataggg gaagaggcat tctttgaaga agttttgact
     4381 gttattgaag gcatagataa gtaagttttg gagacgtgaa tttatagtat ttaaatcaat
     4441 gatttagata ctataataag ctaatattaa ctaaatattt gtggaaaaaa cattttatat
     4501 gttgtcataa attaaaaaat tataaaaata gtcacttaag tattaaatta agcttataaa
     4561 tatttataag taggagggaa aaagatgtta gaatttatga aatctataat cgatacattt
     4621 ggttctgcaa ttatagttcc aattataata ttcataatag caaagatatt taaagtaact
     4681 actaagaaat cgtttctttc tgctgtgtat gcaggagttg ctttacaagg ttttacactg
     4741 attttaaatt catttacacc aataataaca ccagttatca ataggatggt tgagagtaca
     4801 ggagtaaacc taccagtttt tgatgttggt tggcaagcaa catcattggt tgcattttca
     4861 acaagtgcag gtatgatata tttaggtctt ggtatattat tacaaacaat tttatttttg
     4921 ataaaatgga cagacgtgtt tcaaccatca gatttatgga ataactattc ttatatggta
     4981 tggggagcaa tggttatagg agtaacagga aattttcctt taggtatagc atgtatggtt
     5041 ttattaaact tatatagttt attaatatca gagttagttg caaagagatg gtctagttac
     5101 tatagatatc ctaattgtac aataatagct atgcataatg ttgaggcttc tgtgtttgca
     5161 gtattttcag accctatata caataaattg ggattaaata aaataaaatt aaatccaaaa
     5221 gaattagaga agaaattagg gtttttaggt gaaccaatta ctttaggtct ttttttaggt
     5281 atgtttatag gtatattagg taatatgacg agaataaata ctatggaagc ttggggagaa
     5341 ataatgaaag taggtatatc tacaagtgca gtgatggcga ttttccctaa agtagcaagt
     5401 atgtttgctc aagcatttgc acctataaca gaagcagcta gaaagattat gcaaaaagct
     5461 ggaaaccgtg agtggtacat agcagtaaat gatgctgttg gatatggtga acctgcaact
     5521 ttaatatcag gtcttatatt aattccaata atgcttgtaa tatctatggt acttcctggt
     5581 aataaggtat taccagttgt ggatttgttg gcaattccat atatggttca aggtttagtt
     5641 gccatacata atggtaatat accaaaagta ttagtttcag gtataatttg gtttggatta
     5701 ggattatatg tttgtacatc tacagcacca ttatttacag atatggctac aaatataggt
     5761 gtagctatac ctgcaggagc aatgttaata acaagcttta atatactagg aaaacctttg
     5821 atgggattag ttttctttgc atttctaagt gcaaatccaa tttatatagg tctttcagtt
     5881 gtaatttact ttgttctatg ggcattgttt agaaagaaca aaacaagtat tcttgattat
     5941 ttagaaaaac aagcacttaa aaatgtagaa gaagagcctg tagcagttta agtttataaa
     6001 aatgaaataa gaataattaa aggagtttat aattgatttt atataagtat cagataatac
     6061 ctgagaaata tttaaatgaa atatagactt ctttttattt gataatattt gtaattataa
     6121 tgcattgtat ttttatagtt gagttttctg tataacctgc tatttaaaac atactctttt
     6181 atatttatac agtaatgtgg tttaatttta taaaatgtaa tctctactgg taaaagactt
     6241 tgtatataag gcatttgcct gaataaacca tttatctttt gtatggcata gatattgcta
     6301 tatagaagta tatagcatga aaggagatat ttatatggga gataaaatga gagcatcagt
     6361 attgtataat gtaggtgatg tcaggtatga aatggtagat ataccagaaa taacagatac
     6421 gcaagtgctt gtaaatgtaa aatatgttgg gatatgtggt tcagatttgc ctaggtctat
     6481 ggtaagtggt ttgagtggaa atacaaaata tccattgatt ttaggacatg aattttctgg
     6541 tgaagttgtt aaaattggag aaaaagtaaa gcatattaat gtaggagata gagtagctgt
     6601 tgcaccatta gtaccttgtg gaaaatgtga ttattgtaat gaaggtaatt ttggcttgtg
     6661 tgatgattac aatataattg gaacaagagt aaatggagcg tttgcagaat atgtaagagt
     6721 tccagaagaa catattttaa aactaccaga tacattggat tatgaaactg ctgccggaat
     6781 tgaaccagca actatagctt atcatgggat ttcaaagtct aatattagag taggagatag
     6841 tgtagttgtg ttaggatgtg gtccaatagg tcagtttgtt atacaatggg caaaggtgtt
     6901 tggtgcatct aagattatag cagttgatat ttttgatgaa aaattagaac tttctaaact
     6961 tctaggagca aactatatac ttaactcaaa agaagtaaat gtaatcaaag aaataaaaaa
     7021 aataacaaat ggtggtgctg atgttgtaat agagactgct ggtagccgat ttacacaaga
     7081 acaatctcta tttgtagcaa aaaagagagg aaatatagtg tttgtaggta tatcacacac
     7141 agaattacca cttagtgcag acgctactga gtgtatatta cgtggagaac ttaccttgaa
     7201 aggttcttgg aattcatatg caagccctta tcctggaaga gcatggactg caacattgga
     7261 ttttatggga aaaggtgaca ttatttttaa acccatgatt tcacataaaa taggtcttaa
     7321 tgaagtgggt gactttttaa gtaaaatgag caagagagaa attaatttca ataaaatcct
     7381 agtagaaata taaaaattta ataaaaaatt ctaaaaacaa ttaaatttta taactatgac
     7441 attaaataaa atataggagg aagaaaagtg ctagaaattt taaaagaaga agtattaaaa
     7501 gcaaacttag aattacctaa gaaaaagcta attacttata cttggggaaa tgtaagtggg
     7561 atagatagag aaaaaggatt agtggtgata aaaccaagtg gtgtagagta tagtaatatg
     7621 actgtagaag atatggttgt agttgattta gagggaaatg tagttgatgg caaactaaga
     7681 ccctcctcag atactccgac tcatctagtt ttatataaag agtttgaaga attgggggga
     7741 atagtacata cacattcaag ttgggcaaca atttgggctc aaataggaaa atctatacca
     7801 tcttgtggta ctactcatgc agattatttt tatggaagca taccatgtac cagaaaaatg
     7861 actagagaag aaatttgcaa tgaatacgaa aaggaaactg gaaatgtaat cattgaagaa
     7921 tttatgggaa gaaatccaat tcattgtcca ggaataattg taaatgacca cggacctttt
     7981 acatggggaa aagatgcaaa tgaggctgta cacaatgcag tagttttaga agaagtcgca
     8041 aagatggctt attatacaga gttaatgagt cctgataata ttatggataa ggttcttatg
     8101 aataagcatt tttcaagaaa acatggaaaa aatgcatact atggtcaaaa atgattatct
     8161 ggaggtgaat tatgctagac tctttaaaag gtaaagttgt ggaaatagca attaaagcag
     8221 agcattatgg tttatgtaaa gaaaagactg gtaattttag tattagagat aaggatactg
     8281 gatatatact tataacccca tcaggtatag gaagagaaga attaaacgtt gaacatattt
     8341 gtgtattgga tttgaatgga aatgtaatag aagttgagaa aggtattaag ccaagtagtg
     8401 aggtattgat gcatattgaa atatataaaa taagggaaaa tataaatgct atatcacata
     8461 ctcattcttt atatgcaaca gcattttcag tagcaaagaa accaattata ccaattgtat
     8521 atgaatcagt taattatggc ggttatgtag atattgctcc atacgaaaga ccaggaactt
     8581 taaaattagc taagagcgtt tcaaaattac tgatagatac agatgcatgc ttattagaaa
     8641 gacatgggtt gacaactgta ggtaatgatt tagacgaagc attattaaaa tcaagatatg
     8701 ttgaagaggt agcagaaata tactataggt ctttagtatt aaatcaattt aaagaacttg
     8761 aagtagtaga tgttgatgag tttgaatctt ggaaataccc agaagaaata aagttttaat
     8821 ctaaataatt ttcattgaag tggttaatta aaatatatgt attatttata tcaattacta
     8881 tgttttatta tattaatcac cgtattttgt aaatatattt attacatttt ttcaaaaaaa
     8941 tatagaatcc gaaagccata taaaattgac ttgggattct atacttttta tttatgcatt
     9001 tttctttatc tgattttctg gagaatttat atgttcaaaa tattttttag tttcagatac
     9061 tattataggt gataatgcaa gtaaagctat aaggttaggt attgccataa gcccatttac
     9121 aatatcagca attatccaaa caacttctaa ctttaagaaa gcacctaata acaccatagc
     9181 cacaaataca gttctatacc cattcatgcc ttttacacca aacagaaatt caaaacatct
     9241 ttctccataa taactccatc ccaaaattgt agtaaatgca aataatgtta aactaactgt
     9301 aagtaatatt ggtccaaatg taggaattgc tccgttaaaa gctgcttgtg tcataacagc
     9361 tccatttagg tctgaacacc acactcctga aataacaaga gagaatccag taagagtaca
     9421 gattataata gtatctataa aagtacctgt catagatata agaccttgtt ccgctggcca
     9481 ttcagtttta gcagcagctg cagctattgg agcactacct agaccagact catttgaaaa
     9541 tacaccacga gcaataccat ttctaattgc cagtgacata gttgctccta aaaaaccacc
     9601 agtagcagca gtaggtgtaa atgcactttt aattattaac ataaaagttt cagggatttt
     9661 attccaaaaa gatattaaaa tgattccaca gattacaaca tatataactg ccataaaagg
     9721 tactatttta gtagctacat tagatatact tctaagtcct ccaaatacaa ccaaagctac
     9781 aaatactgct aatacaaccc ctgtaattct agggtctatc ccgaaactat tatttattgc
     9841 atctgttatt gaatttactt gagtaaaagt accgattcct aaaagtgcaa ctagtatacc
     9901 acttatagcg aaaaatattg caagaggtct ccatttttct cccataccat ttactatgta
     9961 atacattggc ccaccagaca cttgaccatt tttatcttta gttctgtatt ttattgcaag
    10021 aacaccttct gaatacttag tagccattcc aaagaatgct gctatccaca tccaaaaaag
    10081 tgcaccaggt ccaccagctt tgatagctgt agcaacacca acaatattac cagtaccaac
    10141 tgtagcagct agagcagtac ataaggctgc aaaacttgaa acatcaccac tacctttgtt
    10201 ttcagcagag aatattaact taagagctct tggcagttta gttatttgaa caactccaag
    10261 cttaacagta agtagtatac cagtaccaac taatagtacc agtaggggtg gtccccaaat
    10321 aaaagcatca actttgttta aaatatcaat tagattcata aaaacactcc ctcatttttt
    10381 ttgctttaca taatcaatag aatttttaat gaagtaattg acaggaaata agtataattt
    10441 attaataatt tctagtgtaa taaaaaaagc taaatctaaa aaagattcag ctctggatgt
    10501 acactaatta gcaaccaaaa taaaagtatt agtaaatact gtttttggta gataatccct
    10561 gtccttttgc ctgagagatt gagcataaaa gccttgcccc ttcggcgccc ataatgggtc
    10621 tctccagagt tccgtaaatt ttacagtcct acttttaaaa gtacctgaga gtgttactcc
    10681 ttcggtgtgc tatatagcaa ctcttctata aaatcttaac cggatattga tttgtcatag
    10741 cttattgtat taataatatt aatataagtt tttatgtttt gtcaactaaa aaattaaaaa
    10801 acaaagaaaa aatataaaaa tgtaagccta tttacgaatt ataaacttag aaagatgagt
    10861 aatatacgta aaaatgaatt ttgtaataca catacaaatg aagtgtaaaa taagttttaa
    10921 ataaaaaaat aataaaatct taaataatta attagatatg attaaattat ggtaatatta
    10981 tagttacgat attaagaaaa gttctattta gttagatttt aagcgtaata tgagattatg
    11041 tatagagaaa ggatgtattt ttagatggga aaaatattag tgctagcaga aaaaccttct
    11101 gttggaagag atatagcaaa agttttaggg tcaaaaaatg aaaaaaatgg ttatatagag
    11161 gggccaaagt atgtgataac ttgggcacta ggtcatttgg ttacacttgc tgaccccgaa
    11221 agttatggag aaagatataa atcatggagc ttagaagacc tgccaatact tcccaagcac
    11281 cttaagactg ttgtaataaa aaagagtgga aagcagttta atacagttaa atcgcaaatg
    11341 aatagaaatg atatagatga aatagttata gcaacagatg caggtcgtga aggagaactt
    11401 gtagcaaggt ggataataga aaaatcacag gtaaaaaaac ctataaaacg tctttggatt
    11461 tcttcaagta cagacaaagc aataaaagac ggatttgcaa aattaagaag tggaaaagaa
    11521 tatgaaaatt tgtattactc agcaatagca cgagcagaag ctgattggat tattggaata
    11581 aatgcaacca gagctttgac aacaaagtac aatgcacagc tttcttgtgg aagagtacaa
    11641 actcctactc ttgcaatgtt gcttaaaaga gaagaagaaa taagaaattt caaacctaaa
    11701 gagtattatg gattagaatt aattgctaca aagggaaatt cagatataaa atttatttgg
    11761 aatgataaaa acaataattc ttcaactttt agtaaagaaa agatagaatc catattaaaa
    11821 aaagtaaaag gtgtagacat aaaaatatct gaagtaaaca agtcatataa gaaaaaatat
    11881 tcacctgcac tctatgattt aacagaatta caaagagatg caaataaaat gtttggattt
    11941 tcagcaaaag aaactttatc tataatgcaa aaactgtatg aacatcataa agtacttact
    12001 tatcctagaa ctgattctag atatttaaca tcagatattg ttggaacatt aaaagataga
    12061 ataaaagctg taaatattag tgattattca aagatttgta ataaattatt aaaaagcaat
    12121 attatagcta ataaatcgtt tgttgacaat tcaaaggtaa cagaccacca tgcaataata
    12181 ccaacagaac agaaggtatt catgagtgat tttagtgaca aagagcgaaa aatatataat
    12241 cttgtagtaa aaagattttt agcagtacta tctcctcctt ttgaatatga gcagacaact
    12301 ataagtgcaa ctgtagaagg agaaatattt aaggctaagg gaaacaaagt aataaattta
    12361 ggttggaaag aaagttataa agatattgaa gatgaagatg agtatgaaag tataccaaat
    12421 gttttagaaa aagatgtact taaggtcaag ggattaaaga taacttcagg aaaaacaaat
    12481 ccaccaccat acttaaatga gggaacatta ttgactgcta tggaaaagaa tggtctaggg
    12541 acagttgcta caagagcaga tataatagaa aaattattca attcattttt aatggaaaag
    12601 aaaaataaag agatatatat aacatcaaaa ggaagacaat tattagattt agttccagta
    12661 gacttaaaga caccagaact tacagcttca tgggaaaaga aattattgga tatttcaaag
    12721 ggaaaactta gtaaaaatac attcataaat gacatgaaag attattcaaa agatattgta
    12781 ctagaagtaa aaaatagtga taataaattt aaacatgata acttaactaa aaatagatgt
    12841 ccagaatgtg gtaaatttat gttagaggta aatggtaaaa aaggtaagat gttgatttgt
    12901 gaagatagag agtgtaatac tagaaaaact atatcacaag taaccaattc aagatgtcct
    12961 aactgtcata aaaaattaga acttcgtgga gaaggtgagg ggagaatatt cacttgtagt
    13021 tgcggataca gagaaaagtt gtcttctttc aacaagagaa agcaagaaga aaaacagaag
    13081 gcatcaaaaa aagatgttaa tcaatattta aaaaatcaaa ataaaaatca agagacattt
    13141 aataatccat ttgcagaagc attagctaag ctaaaaaaca aataatttta aagttattat
    13201 tatacacaat tataacttta actgtatatt agttatatgt actgatataa tatagtgtat
    13261 agtttaggca tgaaagtagg gctgttaaat ggatttaagt ggcatattta aatactattg
    13321 taaagagtgt gaaaatacat ggaataattc gagtgttgaa ttatttgagg atatagaaac
    13381 gtatagtaaa gattcacaaa aaaagaggga aaaagaatta gataaattta taaatacaat
    13441 atcagttcat ttagagaggt atccaagtga tgctgtattg agaaaaatgt gggtaaaaaa
    13501 gggcgaggtt ttcttacaaa agacattgga aaaagaaaat atttttaagt tagaaaaaat
    13561 ggatgtagag gatagaaaaa aatttttaga tataacaaaa cagtttatta gagatgctag
    13621 aaaatttgat gatgatttac ctataggtga tattatgcaa gctatgagga atgtatggat
    13681 ttcaaatgca ttacaattat tatttggtaa agaaatatat tattcaaaag ctaactttgc
    13741 atatagtatg ttatatccat atactgataa ttatttagac aatacaaata tagataaaaa
    13801 tgataagatt ttatttaata actggttaga aaaaaggctc ctgggagaac atattaaatc
    13861 taaggattat catgaaagta aagtatctca aatgatagat tatattgaaa gtgtataccc
    13921 tagagaaaag tttacacaag tttatgaatc gttattatta atatttaaaa gtcaagtaaa
    13981 tagtttaaaa caacatggta aggaaaatca tttgtgtaaa gaagatttat tatccatttc
    14041 tattgaaaaa ggaggttcat ccgttttagt agatggatat ttaataagtg gatttatgac
    14101 aaaggaagaa atagagtttt gtataggata tggattttta ttacaaatat ctgatgattt
    14161 acaagatata aaagaagatt taaaatacaa ccataaaact attattacag agatgtcaaa
    14221 agagggtact ttagataaag ttgtaaataa actaataaat tttactattg agttaataga
    14281 tagttttaaa attaataata aaaataaatc tgtaataact atgataaaga atgattgctt
    14341 aatgttaatt ttattttctg tagtttataa tgctgaattt ttttctgtag gatatataaa
    14401 agaagtagag aaatttattc catatacaat agattattca ttagagattg aagaaaaaat
    14461 aaaagaaaaa tttaaaaata tagatgtttt aaataatgaa aatgaatata aagaaatgat
    14521 tgatattatt tgtgcagagt agaaaaaaag ggagtgtctt agaatagact ttttagttta
    14581 tgaggcactc tttttgtata aaatcaaatg tattttcatc attttaacaa ttaaaaagag
    14641 cttatccctg tttgagaaag ccctttttaa tttaaataaa aatgtcaagg ttaaactatt
    14701 taaatgcagt aataatccca ttatcatgta agttttttat gattgtagct gcgataggga
    14761 atccaaataa tccagctatt ccaaatagtt gagctccaat aaacatacac attaatgtta
    14821 ttaaaggata taggccaatt tggtgtccta ctacctttgg ttctaatatt tgtctaacaa
    14881 cggttataat tatgtatagt attaaaagtc ctattgctaa attaatattt ccattaatga
    14941 gtgagataat catccaaggt gtcaaaatac ttcctgtacc caagactggc aaaatatcaa
    15001 caagtgctat gatgacagat atagtaaatg cattttcaac ctttaggata gtaagtccaa
    15061 taagtagttc taagaatgtc actgatagta gtattgcata ggcttttagt agttttaaaa
    15121 gagcattaat actgctattt tttatattta ttatcataaa ttgacttttt tctggaaatt
    15181 gttttaagat aaagtttact attttttgat agtcaaaagt aaagaaaaaa gaagcaatta
    15241 aagtaataat aagttttaac ataaatgcag gtgttttact tgcaatacta gcaatagcat
    15301 tgagtgcatt tgttgaaata gaagctacta aagacatcat agattgactt atattatccc
    15361 aaccaagtat taatgatagg ttaacattag gtgtactagc atctatcttt gaaaataaag
    15421 tatttagcgt tggttgtata cttgatttat atatttcagg aagtctaaaa aatacatcac
    15481 ttacacttgc aaatatcttc acaccaaagc taactaataa aaatatacac aacccataaa
    15541 aaattaaaag tattactata gaaataagtg tacgatttaa atttgtatta tttgtaataa
    15601 gtcttataat aggtcttaag atggatgcaa tgataaaact gattacaaaa ggcattaata
    15661 atggcaataa gaattttaat acaatatata caatacttgt tattaaaact acatatgtaa
    15721 atttaattat aaaattcttt tgcttttgaa tatccataat ataaaacctc caatgtattt
    15781 gatattattc taaatatggt cttttttaca gaaaatatac tactgtatat tgattataaa
    15841 ctataaagct tatgtaaagt aaataaatta tgtaaaattt aggttaattt tgtataaaga
    15901 gatactttta tattttacaa ttaataacca aatactaaaa aatattttaa ataaagaact
    15961 taaaattgtg ataaaattaa tttatagata ttaaaaaaag tataatatat ggtttttaag
    16021 aggtgctaag atgaaaaatt ttagagattt aggtgggaac aaaactgagg atggcagaac
    16081 tgtaaagaag ggattatttt ataggtctgc aaaattatca aatctaagtg aaaatgatat
    16141 aaagatttta aaagagttaa atattaaata tatatttgat tatagaagtg atgaagaagc
    16201 tcgtaaacat ccatcaacaa taatatcaaa tataaaaaat ataagaatac cagcaatgag
    16261 agagctagaa gaatcaggag gaagttttgg ttctattgaa gatatgattg atggattgtt
    16321 tgaaaaagat ggtgcattta atatgttaaa taatagttac tataatttgc ctataaataa
    16381 tccgtcatat aaaaaattag tagaacttat aagagactat tctaatttac caatattaaa
    16441 tcattgtaca gctggtaagg atagaactgg tgtggggagt gctataattc ttatgatttt
    16501 aggggtatcg agagagaata taatgaaaga ttatctaaaa agtaatgatt ttgcagataa
    16561 agagatagaa agatttatag aatataagcc taaatttaag gacattccaa aagaaaattt
    16621 aaaatatata tttggtgtaa atgaagagta tatgaaaact gcatttagaa gaattgatga
    16681 agaatacatt agtgtagaag catatttata tggagaattt aatttaaata aagaagaaat
    16741 aagaaaatta agaaatcaat atctagagta aaaggtgagt ttatgagttt aaatataaca
    16801 gcaagacaga taaatataat aaaaatattg ttaaattcaa aagatacaat tagtggtatt
    16861 gccttaagtc aagagatagg atgttcttca aaaactatac aaaatgaaat aaaggatatt
    16921 aataaacaac ttagaaatgg taaaattttg tcaataagag gagttggtta taaactagaa
    16981 ggaagttttg atgaggtaaa tctaaatagc aatctctatg atgatgtaga tagagtagaa
    17041 tatataatca gaaaaatatt aaccttaagt aatgaagaaa aaaatactat aaaacttgaa
    17101 gatttagccg attctatgta tgtaagtgtt tcaacagtaa aaaatgattt aaaagaagta
    17161 aaaagaatat tggaaaagta ttcaatatca gttaaatcta agcataaaca aggtatatgt
    17221 gttttggaaa gtgaagataa gatattaaat tgtatagttg acttaagcaa taagagagat
    17281 aatcaattga gtttaaatga ttttttaaat gacgatataa aggaaagaaa actattaata
    17341 aaaaaattat tattagacat attaaatgaa aatggattag tattaactga tatagaattt
    17401 aagagtgtgt taaataatat attagttatg ttaagcagac ataagtataa tgaaaaagaa
    17461 tttatagaga attatataga aaattatagg agcaaaagaa gctcaatttt agaaaatgaa
    17521 caaaacaaag aaattataat aaattcaata aaaacatttt gtaaaaattt aaaattagct
    17581 acatcaatag atataagtaa tgataaggta tttgaagagt ttttatataa gcatattagt
    17641 agtttatgta aaaaaataaa gttaggtata aatcaatcac ctataatggc tcaagatata
    17701 aaaataaaat atccatttgc ttttgaacta gctaatatag ctaaaaagac aatagaaaaa
    17761 gatttgaaga tagaaattaa tgaagatgaa gttgcaaaca tagctctaca tataggagga
    17821 gcagttgaaa gagcatctta taataataaa gataaggttt tcaaaactat aatagtttgt
    17881 acttcaggca taggcacatc aatgttaata aaagctaaat tagagaatat atttaaggaa
    17941 aaattagaaa tactaaagat tattccttca tacttagttg aatatataaa agttatagat
    18001 gttgattttg taatatctac tgttcctatt gaagtaggag atgtgccagt aataaatata
    18061 tctccaattt taagtgagaa agaaattaga ttaattgaaa aatatataga aacaggaaat
    18121 atatacctag atttagatat aaaaagcctg tttgatagtg aattattttt tactgatttg
    18181 gtgtttaaaa ggaaagaaga agttatagat tttatggcaa gtaaacttgt acaaaagggc
    18241 tatatagatg aggatatgaa aaaatcttac tttgagagag aaaaaatagc aacaacagaa
    18301 atagggaata tggtaactat accacatgga gctattggaa aaatttttga taataaaata
    18361 gcaattggta tattaaagaa gcctatttct tgggaaatat cagatgttcg tttaataata
    18421 atgttagccc ttgataaaga taaaatatta gattatgaag taatattttc aaaaatatat
    18481 aaaagagtag attctatagc aaaagttata agtatatgtg aaaatagtag ttttgaaaaa
    18541 tttgtaaatt tatttaatta gatgggtagt attgtatata cacctgtaaa tttggataat
    18601 ataaaactaa ataagataaa aacgataaat ttctctttta attaagagaa attttttgac
    18661 tttcaaggat attaatataa agttaagata taaacataaa gcaaataaat aaaaaagagg
    18721 tggcaatatg atttatacag tgacattaaa tccatcaata gattatatag ttaagctaga
    18781 tgagctaaag acaggaagta caaatcgtgt aaaagaagaa tacgtttatc caggaggaaa
    18841 aggtataaat gtatctcgca tacttaagga acttggaaat gataatacat ctttaggttt
    18901 tataagtggt tttactggag aatatataat aagaacattg gaagaaaaag aattgaagac
    18961 agattttata aagataaaaa gtggtttttc aagaataaat gtaaaaataa aggcgttaaa
    19021 ggaaactgag attaatggac aaggtccaaa tatagatgat gaagacattg atactttata
    19081 taaaaaactt gataagctaa atcaagatga tattttaata ttggcaggaa gtataccatc
    19141 tactttagat gaaaaactat acgaaaatat aatggctaga ttagaaaaaa agaatataaa
    19201 agttgtagta gatgcaacta aaaatcttct tcttaatgtt ttaaaataca aaccttttct
    19261 aattaagcct aataacgacg aattagaaga actatttggt gttaaattaa attcaattga
    19321 agacatggta aaatatgcta aaagattaaa ggaaatggga gctataaatg tcttggtatc
    19381 aatgggaaaa gatggagcat tgttgattac agaggaagat gaagtgctta taagtgatgt
    19441 ccctaaagga aaagtaaaaa attctgtagg agcaggtgat tcaatggtag caggatttat
    19501 atctgggtat ttgaatactg ggaaatatga ttatgctctt aaattgggag cagcttcagg
    19561 tagtgcaact gctttttcat atgacttagc caaaagagaa tacattgata aattggtaaa
    19621 tgagatatct gtaaagcagt tttaaaatat taaaaaatat atagaaaggt aattaatatt
    19681 tatgaaaatt atagatttat tagatgaaaa gtcaattaag ttaaatttaa attcaaaaac
    19741 caaatcagaa gcaatagaag aattagtaga tttggtcgca aatagtggaa atttaaatga
    19801 taaagaaaac tacaaaaaag caattttggc tcgtgaagaa atgagtacaa caggaattgg
    19861 agaaggtgta gctatacctc atgctaaaaa ttcttcagtt actaaagctt gtatagcagc
    19921 agcagtgtca aaagaaggta tagattatga atcgtttgac ggaagtttat caaatctatt
    19981 ttttatgata gctgcaccag acggagctaa caatactcat cttgaagtat tatcaagatt
    20041 atctacaata cttatggatg aagactttag aaataaactt ataaattcaa gctctgaaaa
    20101 agaatttaag gaaataatag ataaaaagga aagagaaaaa ttcagtgaag aatatcaaga
    20161 tgagaaagtg gctgaaaaaa atataataga tactaaagag aatgatgcta ataagtatcc
    20221 taaggtttta gcagtaacag catgtccaac tggaatagca catacattta tggcagctga
    20281 aagtttaaat aaaatggcag aaaacaaagg tgtatcaata aaagtagaaa ctaatggttc
    20341 agcaggtgtt aaaaataagc ttacaaaaga tgaaatagaa aatgcaacat gtataatagt
    20401 ggcagcagat aaaaatgttg agatggctag atttaatggc aaaaaagtca taaaaactaa
    20461 ggtggctgat ggtatacaca aagcggaaga acttatagac aaggctgtaa atggagatgc
    20521 tccaatttac catggaggag atggaagtca caacgaatca aatgaagaaa gtgaaagtgg
    20581 atttagaaaa gtatacaaac acttaatgaa tggtgtatct aatatgttac catttgttat
    20641 tggtggaggg atacttatag cgatagcctt tttacttgat gattatacta ttaacccttc
    20701 aaactttggt tcaaatacac cgatagcagc gttttttaaa gggataggag ataaagcttt
    20761 tggatttatg ttacctgtac ttgctggata tatagcatac tctatatctg atagaccagc
    20821 gtttgttgta ggatttgtag gaggtgctct agcaggtgat ggtggttcag gatttttagg
    20881 tgcactactt gcaggtttta tagcaggtta tttagtagaa gggcttaaga aaatatttag
    20941 tgtactacca gcttcattgg aaggaataaa accagtattg ttataccctt tacttggaac
    21001 acttttgatg ggaattataa tgacattttt agtaatacct ccagttacag caattaataa
    21061 tgcaatggtt ggatttttaa atggtttagg cggaacttca aaaatagttt taggattagt
    21121 attaggtgga atgatggctg tggatatggg tggacctgta aataaagcag catatgtatt
    21181 tggagttgca tcacttgaat cagggcagtt tgaaataatg gcagcagtaa tggctggtgg
    21241 tatggttccg ccacttgcta tagctcttgc aactacattt tttaagaata gatttactaa
    21301 agaggaaaga gattctggta aagttaatta tgtaatggga ttatcatttg ttactgaagg
    21361 agctatacct tttgcagctg gagacccact tcatgttata ccagcttgtg ttggaggttc
    21421 agcagtggca ggagcactta gtatgttgtt caacgcagct cttcgtgcac ctcatggagg
    21481 agtatttgtt ataccagtgg ttacccatcc atttgcatat atattagcta tagctgtagg
    21541 tgctttggtt ggaatgatgc ttttagcatt acttaaaaaa ccattaaatc aagaagtata
    21601 gcaattaaag gatattcaag aacttaaaat atgatgtttt ataaaaatta atatattgct
    21661 gaattatttt aaaataagga ttttattaaa ataaggattt tattaaaata aggattttat
    21721 gaggctgtta tataacgatt tgtaattgtt atcttgacag tcttttattt ttatataagt
    21781 aaaataagtg tgttttgctt tatttgtatt taaattgttt ttaagttaga atataaataa
    21841 a
//
LOCUS       DAEPOG010000056        21533 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEPOG010000056 DAEPOG010000000
VERSION     DAEPOG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.056"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            103..987
                     /locus_tag="KR432_001737"
     CDS             103..987
                     /locus_tag="KR432_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0640277.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1086..2261
                     /locus_tag="KR432_001738"
     CDS             1086..2261
                     /locus_tag="KR432_001738"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBH0640278.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2389..3243
                     /locus_tag="KR432_001739"
     CDS             2389..3243
                     /locus_tag="KR432_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640279.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3287..4522
                     /locus_tag="KR432_001740"
     CDS             3287..4522
                     /locus_tag="KR432_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH0640280.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4623..5564
                     /locus_tag="KR432_001741"
     CDS             4623..5564
                     /locus_tag="KR432_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBH0640281.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5793..6146)
                     /locus_tag="KR432_001742"
     CDS             complement(5793..6146)
                     /locus_tag="KR432_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0640282.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            6442..7353
                     /locus_tag="KR432_001743"
     CDS             6442..7353
                     /locus_tag="KR432_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBH0640283.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            7613..8047
                     /locus_tag="KR432_001744"
     CDS             7613..8047
                     /locus_tag="KR432_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640284.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            8070..9605
                     /locus_tag="KR432_001745"
     CDS             8070..9605
                     /locus_tag="KR432_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640285.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            9689..11056
                     /locus_tag="KR432_001746"
     CDS             9689..11056
                     /locus_tag="KR432_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640286.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            11091..11315
                     /locus_tag="KR432_001747"
     CDS             11091..11315
                     /locus_tag="KR432_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBH0640287.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(11765..12163)
                     /locus_tag="KR432_001748"
     CDS             complement(11765..12163)
                     /locus_tag="KR432_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBH0640288.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(12166..12567)
                     /locus_tag="KR432_001749"
     CDS             complement(12166..12567)
                     /locus_tag="KR432_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0640289.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            12926..13912
                     /locus_tag="KR432_001750"
     CDS             12926..13912
                     /locus_tag="KR432_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640290.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            13896..14807
                     /locus_tag="KR432_001751"
     CDS             13896..14807
                     /locus_tag="KR432_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640291.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            14920..16488
                     /locus_tag="KR432_001752"
     CDS             14920..16488
                     /locus_tag="KR432_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640292.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            16563..17588
                     /locus_tag="KR432_001753"
     CDS             16563..17588
                     /locus_tag="KR432_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640293.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            17581..18525
                     /locus_tag="KR432_001754"
     CDS             17581..18525
                     /locus_tag="KR432_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640294.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            18884..20542
                     /locus_tag="KR432_001755"
     CDS             18884..20542
                     /locus_tag="KR432_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640295.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            20523..>21533
                     /locus_tag="KR432_001756"
     CDS             20523..>21533
                     /locus_tag="KR432_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640296.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDY"
BASE COUNT         8631 a         2175 c         3804 g         6923 t
ORIGIN      
        1 atatataaat atgtattgac atataaatat tatgaattac aatttatata aactaataaa
       61 aattaaataa tattaatcaa cttattgaat ggaggaataa aaatgaaatt tgtgactttt
      121 tgtagtagca atgaagaaaa aataggtgta tttaattcag aaacaaatag tatatatgaa
      181 attaatagtt taggattaag taaattatat actgatatga atgattttat tgaaaacgta
      241 tctacaggag atttagaaaa aataaaaaac aattcatttg aaaacgcaaa gtgttataag
      301 ttagaagaag ttaaattatg ttccccaatt gtaagaccta aaaaagatat tatatgttta
      361 ggtctaaact ataaagacca tgtcaatgaa atcccagatg gagttataaa aaacgtagtt
      421 atgccagatt atccaattta tttttctaag agagcagaca aaataattgg agtggatgat
      481 aagataagct tacatggaga tttggtagag aagttggact atgaatcaga acttgcagta
      541 atcataggta aagaaggtat aaatatatca aaagaagatg cttatgaata tatatttgga
      601 tatacaatag taaatgatat aagtgaaaga gcacttcaag ataaacatgt tcaatggttt
      661 agaggaaaaa gcttagacac acatacatct atgggacctt gtatagtaca taaagaagaa
      721 tttgaacatc ctttaaaatt agatataagt agtgtagtaa atggagaagt aagacaaaat
      781 tctaatacag agtactttat atttgatata cctactataa taaatgattt atcaagagga
      841 atgactctaa agccaggaga tataatttca actggaactc cagcaggggt tgctatgggg
      901 atgaatcctc aagtatattt aaagcatggc gatatagttg aatgtaaagt cgaaggaata
      961 ggagttttaa aaaatatagt tgattaatgg ataaatatgc catataattt tattagtaca
     1021 aataatttaa ttaatatagg attgatcaaa cttatgatta atttattata gagagggatg
     1081 agtatatggc aagagaacta gaattaaagt atggctgtaa tccaaatcaa aaaccatcaa
     1141 agatttatat gaagaatgga gaacttccta tagaagtatt aaatggaaaa cctggatata
     1201 taaatttttt agatgcattt aatagttggc aacttgtgaa ggaattaaaa gaagctacag
     1261 gtcttccagc agcaacttca tttaaacatg taagtccagc aggagcagca gtaggggtac
     1321 ctttatcaga tactttaaag caaatatatt ttgttgatga tttagagctt tctccattag
     1381 cttgtgctta tgcaatggca agaggagcag atagaatgtc ttcttatggt gattttatag
     1441 cactttcaga tgtatgtgat aaggagactg caactattat agcaagagag gtttctgatg
     1501 gtataatagc tccaggatat acagatgaag cccttgaaat attaaaagga aaacgtaaag
     1561 gcaactataa tattgtaaaa atagatgaaa attatactcc tgagcctatt gaaacaaagg
     1621 atgtttatgg aattactttt gaacaaggta gaaatgaaat attaataaat gaagaactat
     1681 taaaagatat tccaactgcg aataaaattt ttacagatag tgcaaaacgt gatttaatta
     1741 tagctcttat tacattaaaa tatacacaat caaattctgt ttgttatgct aaagatggac
     1801 aagtaatagg ggtaggtgca ggtcaacaat cacgtattca ttgtactaga cttgcaggta
     1861 acaaagctga tacttggtat cttcgtcaac atcctaaagt attaaactta aaatttaaaa
     1921 aggatatagg tcgtccagat agagataaca ctattgatgt atatctttct gatgattata
     1981 tggatgtgct tgctgatggc atatggcaaa acttttttga agaaaaacca gagcctctaa
     2041 ctggagaagg aaaaagagca tggcttaaaa ctttaacagg agtagcactt ggttcagatg
     2101 catttttccc atttggagat aatatagaac gtgcaaaaag aagtggggta agttttatag
     2161 cacaaccagg tggttctatt cgtgatgata atgttatatt aacttgtaat aagtataaca
     2221 tagtcatggc atttactaaa aatagattat tccatcatta aaatactgaa agtataaaga
     2281 taatatagat taagtaaaaa tctacaacaa gttttgttgt agattttttt atttgctgta
     2341 taattttaaa tagaacttta tttttagata ttcaatttgg aggttgatat ggctttaaat
     2401 ataagaaact atagcctatt atgtaatcaa ggaacagcta aatttaatga agatgtagtc
     2461 ggtataaatc catttggggc atgggtttta gatggtgcta caggacttaa tggaaaaaat
     2521 ttagtgtcaa atgaaagtga cgcaaggtgg tatgttcagt ggtggaatag gtatttatat
     2581 aaaaatataa ataaagaaga atcattaaag agtattatta atactggaat aatagaagta
     2641 aaaaaagagt atgaactaag attaaatggg tttaaggttg aaaaattaga tttaccatct
     2701 agttctatag ttgttgtaaa atttcatgaa aataaagtag agtatttttt attaggagat
     2761 tgtgctttgt ttattaaaaa tggagattcc aaagttataa aagataggag tatttgtaaa
     2821 tttgataata ctgtatttga tgaaatgagt aaactttcta acttggaaga gatggcattt
     2881 gatgagatta gaactagtgt tatggataca atagtaagca atagattgaa gaaaaatacg
     2941 aaagagggct attggatatt ggattttgat gaggaagctg tggaaaattc tatatatgga
     3001 tacattgaaa taaaaaaaga ttttcaattg atgctaacga gtgatggttt tacaagtgca
     3061 tgtgatagat ataatctaat aaaagaagaa gaattgatac gtatagcaga aaaatttggt
     3121 ataggatata tacataataa agtaagggat tttgaagata atgactataa agcggttaaa
     3181 gtgccaagat ttaaaataaa agatgattca tcttgtttat atttagatat atataatgat
     3241 tagactgtat aaaactattt aattttagaa aggaggcaag tgaaaagtga aaattcttca
     3301 tataataaca caaaaaccaa atagtacagg tagtggtata tatttgtctg gtatgataaa
     3361 agggttcgaa aaaatcggtc ataagcaagc tgtaattgca ggtatagatg taaatgatga
     3421 tgtaaattgt tttccaagtg aagtaagttt ttatccagta aagtataatt gtggggagtt
     3481 aaattttcct gttgtaggta tgagtgattc tatgccatat gaaagtacaa gatataaaga
     3541 tttaaatata gacatgataa atagattgaa atatcaattt aaagtaaata tagataaggc
     3601 tatgaatgat tttaaaccag aacttataat atgtcatcat ttgtacctct taactgcatt
     3661 tgttagggaa atagtaaaag atataaaggt tatgtctatt tgtcatggaa cttgtttaag
     3721 acagttaaat accattgatt tagagaaaga atacataata gcaaatataa ggaaattaga
     3781 tttaatattt gctcttcatg aaaatcagaa atatgatatt ataaagactt ttggagtaag
     3841 tgaaagtaaa gtagttgtaa ttggtagtgg ttataatgat gatatatttt ataataaaaa
     3901 ttataaaata aaggatgata aaataaaaat agtatttgca ggtaagatat gtaaatcaaa
     3961 gggattgatt ccatttataa aatctatatc taaattaaaa tatagtaaag atttaataga
     4021 agtcaatttt gcaggtactg gaagcgatat cgaatcatat aatgagatag ttaaactggc
     4081 aagtaagtct ccatttaaaa tgaatttcct aggaaagtta gaacagagag atttagctga
     4141 gctattcaac aggtcacaaa tatttgtgtt accatcattt tatgagggat taccagttgt
     4201 agttttagaa gctttatctt gtggcacaga tgtaatcact actgatattc ttggtgttaa
     4261 agagtggatt ggtagtgaga ttaataattc tggaaagata gaatatattt ctctaccttt
     4321 tatggagaaa gaaggtattc caaaagatga agagttatat gattttgaaa ataatttgta
     4381 taatgcaata gattctaaga tacaatcttt attgaataac tcaaatgaga aaacatctgt
     4441 agatatgtca aaaaaaacgt gggatggctt agcatataga ataaatgaag ttattttaat
     4501 ggatgaattg tgtttagtct aaaattattt aaattataaa aaagtgtaac tatttttaaa
     4561 ctttttttaa atgattttat attatactaa tactaaatat atttgtttta gggaggtaat
     4621 ttatgaggat tagtaaaaag gtattggcgt taggtatttc agcattactg ttaagtgtta
     4681 gttttccaac atcaataaac gcactagata agattgaaaa tattcaagga gtagataaat
     4741 atgaaacagc tgggcttatt tcagataagc aagattttac aacagcagtt cttatcaatg
     4801 cagattctac aatggtagat gggattgctg caagtggtct tgcaggagta aataatgcag
     4861 ctattttatt aactaataag gatgatatac cagaagcaac acttcaaaga ctgaatagag
     4921 ttactaagat atatgtaata ggtggagaaa attctataag taaagatgta gaaaaaatgt
     4981 tacttatgag aagaatgcaa gttataagaa ttgatggtgt agatagagta gatacaagtt
     5041 ataaaattgc aggtgaaata gaaaaaatta aaaacagtga taagatgttt ttagtaaatg
     5101 gatttaagga tgaagctgat gcagtaagtg ttgcatctgt tgcttataga gatggagctc
     5161 ctataatatt gacaaaagat ataccttcag cagaagaaga cacagactta gacccatggt
     5221 ttggagtttc tcctgttcca gtatatgcta ttggaggaga gtctacatta agtgattaca
     5281 tagtgagcag atatagagca actagaattg gcggtgttga tagatatcaa actaataaaa
     5341 atgttattga aaagttctat aatggagcaa aagagttcta tataacaagt ggagacgatt
     5401 tagtttatgc tttagttgca tcaccacttg caaaaaatgc tcctgttgtt ttagtttcaa
     5461 ataaaagtga taagagtata ttaagtggag ctagtaaagt aacagcaata ggtataagtg
     5521 ataagagtat tatagagcaa tgtttggatg ctgtaaagaa gtaatggagt gttattagta
     5581 aaatgaatat atacattgaa aattagtaga ttgttcattt tataatggta taaaatatat
     5641 gggaagaggc tatcaaatgg agataagcct cttttttatt gataaaatta tgaaaatata
     5701 ctttatttta tataaaaaaa gtatctcatg aactaaatgt ctattttgat atactatctc
     5761 cttttcttat attatatgta ttatatgatt ttttatatgg aataaatttc ataagtatta
     5821 tcttccttta atttttctaa ccaaatcatc tctaaaatat ctagttcttc taaatagtct
     5881 ttctctgttt tgttatcatc atgttttata agttctaaaa cttcaaatgt aaatgctttt
     5941 tcaccatatt ttttccattc attatttaat tcttttatca tacatgagcc agcaccaagc
     6001 tgaaatttga gagagttaaa tttgcttttt atgtcattac tttttccaag gtaaatcttt
     6061 ttagtaatat cattttttat tatgaataca cctgtgtcaa atttcatttc tttgtataat
     6121 tgttttaatt cacgttttct atccatgatt ttagatacct ctctttataa ttgatatgta
     6181 atatgtaagt ttaaaaatat attacatatc aattataaag agattaattt tatatgtaaa
     6241 tagtttttat aaatttaatt atgaattagt catcaataaa caagaaattt agctgtagaa
     6301 atccattata aaggctatta taaattttaa tatgctaaaa atataaaaag aatagttgac
     6361 agtatagaaa atatggaata aactaaaagt gtaatagtaa tacgaaatta tatattacat
     6421 tgaaaggtgt gaatgattat tatgaataaa aaaatagaat taatagggaa ttttgcaagt
     6481 gattggatta ggtatagtga ttatgaatat aaaattactg aagaagatga attatatata
     6541 gttccaacag aagatgctgt gtttaccatg tataacccat ttgatgtagc agatgatata
     6601 atagtagata taattcgcat aggacaagag gcattacaag atatatcaaa agagacaaaa
     6661 gaaatgaaac taaagaaaga agtacttgaa tttgtaaaaa agtatggatt attgggttta
     6721 atatattcaa gtgtatataa tagagatata gttggagaag aaagagtact gatgattgaa
     6781 aagaattata ttacaaaaga gaatatattg agtacagatg aatatgtaaa catgtttata
     6841 ccatttgcaa gtgatgaaga tgtgtatttt aaaaaatata gaaggggtat agatgtatca
     6901 aagagagaag aatctccaaa gttttttgga aagagaccat tgattttaga tttagtattt
     6961 tcaaagttct acactgaaca actagattgg attgtaagtt ttgctaaaga tatgtgtaag
     7021 cattttaatc aaatttgtgt atataaaaat acatctaatc tattaactga aagtgttact
     7081 attatgtctg gagggtttaa tcctcaaaag ataggatttt ctataagcca attagataaa
     7141 ccaattattg catgggagtt tgattctttg aaaacagcta ttgagactat ctatgccttt
     7201 gcaattacaa gtgaaccttc ttctttaaat aaatgcaagt actgtggtaa aatttatatt
     7261 cctacaaatt ctaaatctca atactgttca ccttcttgta gaaattgctc aaatgtaaag
     7321 aaaagtagaa gtagagccaa aaaagataaa taaaattaat aaatatatca ttgtttagat
     7381 tttagtaaat aactactgaa ttaaaagttg ttatagaaaa ttatatatct aaatgatggt
     7441 atttttttgt tgagaaaatt gaaaagttta attgagatag taattttgag ttgacaaaaa
     7501 ccacttttaa cactactatt ataagtggtt ggtaaaataa tatacactaa tttaaatata
     7561 gcataaaaaa caaaaaaagt caatgaaaaa aataaaaaag gagaatttaa aaatgatatc
     7621 acctattatt ttgagttcta taaatcagaa tttaaaggaa atagaaagaa atgaactgct
     7681 tgaaactaat atagaatctg gagattatgg tctagctctt tctgaatctg atgtaaaaga
     7741 tataataaat tcgagagata atactttaaa ggggtatgga agaatagaat tagatataaa
     7801 agttacaaaa cagttaatag aaaatatata tacatcacaa tatacaaatg tggacaatta
     7861 tttagaggct attaatgata tgcaagaaat tttttattat ttaaaaaatg aaactgatga
     7921 caagatatgt gatgatgaga ttatagaaat actgggtgaa ttttatgaaa aattttcagg
     7981 caatatggac aatgtaagag gagaagcaga cgaatttgca aagaaattta aatttggaga
     8041 agtttaatat atgataggag agattttgta tgttaaataa tttaagtaat attaatataa
     8101 acgaaaataa tttaataaag aaccaatatt ccatatctct acttaaggaa tgtctaaatt
     8161 gtaaagtgat agatgaaaga gaagtatata atatacaaca agaaatatct ctaatactta
     8221 tggatttgat taaaaaatat acaaatggtc aaagcacatc agtaaaaaca gaagttgccg
     8281 aaaaactgtt gatttcaata tggtatgcta ttgatgcata tataaataag cttggagata
     8341 tagaaaaaag agttgtggag ttgactgcta agaatgttaa aaaagtgtac cttggaggca
     8401 ttgaaatatt agaggaagag ttagttaggc tgaaagattt ttataaacta atgatgagta
     8461 gaaaattgga tactaattca atagcatata atgatacctt gatggagtta tcaagtttct
     8521 ttaaaagtta tattgtaaaa tttgaagctc aggacgttcc agtaagtata gattatccat
     8581 tggcactaga tgatatggat gttaagggta tttattatgt gaagaattat attgagagta
     8641 tagatataga aaatagattt tgtaacctat ttaagaaaaa agatagagaa aaattatttt
     8701 atgactatgg ggtgatttat aaaatagatt gtgatgttat acttgtaaat ttatttgaac
     8761 ttattataaa taattgtatt ttttctacta ttttaggaaa tgaagtgatt aatttagaaa
     8821 tttcaaaata tgagtatgaa tttttagaaa aacaatttaa aaatatgtac aataatatta
     8881 ctgaagataa agttataggc tctgaagaaa gtaatataat tgaattgatt gaagtagatg
     8941 atagttttaa cactgaagta gatagtaatt tggattataa tgattttgaa gaaattaaca
     9001 gagaaattag aataaataaa aaaataactt tactacttat ggaagcaatt gaagtattaa
     9061 taaaaagatt aagcatagaa gatgaagatg taataagata tattaaaaaa tatgaaaaga
     9121 tatttattga atctgtgcta agctcaataa aaagtaacac ttttaaaagt atggtaactg
     9181 tgagtaaaaa taaaaatgaa cacttagaag actatattgt tgatgatgaa agtaaattgg
     9241 atgatgaatc gtttagagag atattcaatg agatattaga tagtacatct ataattgaaa
     9301 aaataagaat aataaaggaa aatatcaatg ctaaaaaaga ctttatcgat attcttgaat
     9361 ctgaatgttt atttggagaa gagtatttaa tgttgtttgc atcattaagt gaattagaat
     9421 tggcaatatt gggtagtgta gtattttatg aagatattag aatgagagaa attaatatat
     9481 tagaatttat gttaaatgag aaaactgaaa ctactttatg gaagacagag tacattaaat
     9541 ttatgaagag gcaaagtgaa gataagatat actccataga agagcatatg aataaaatta
     9601 attaacaaaa aattgtgaat aaatagaatg ttagtatata atataatctg taaagcatat
     9661 caaaaataca tatatttgga ggaaaattat gaaagagtat atcataaata atgttgaaga
     9721 aataagagaa gaattaattg atttatctaa gaaaatatgg gaaaatccag aattagcctt
     9781 tgaagaaaag tatgcaagta gcattcaaaa agaataccta aagtcaaaag gatttaaaat
     9841 tgaagaagta gaaaatttac ctactggatt tatagcaagt tttggagagg gtaaacctgt
     9901 gattggcata cttggagaat atgatgcttt accagagcta tcacaatgtg tgagtgcaga
     9961 aagaaagcca ttggtagaag gaaaagctgg acatggatgt gggcataacc tgttgggtgt
    10021 agcaggtgta ggagcagttg tttctataaa aaaattaata gaagaaaata aatttaatgg
    10081 aacaataaaa tattttggtt gtcctgctga agaagaaggt ggaggaaaga cagttatgtg
    10141 tataaatggc tgttttgatg atgtagattg tgcatttaca tggcatcctt ttgatataaa
    10201 tgcaccttgg agaggtggaa gtctggcaaa cttatctgta aaatttaaat ttaaagggat
    10261 aacagcccat gcagctcaag ctcctcataa tggtagaagc gcacttgatg ctgttgaaat
    10321 tatgaatgtt ggagctaatt atttgagaga acatgttatt gactctataa gaatgcacta
    10381 tgttataact aatggtggtg gaagacctaa tgtagttcct ggatttgctg aaagttggta
    10441 ctttattaga ggtaaaaaag caagggatgc agaacatgtt cttgatagat taataaaagt
    10501 agctcaagga gcagcaatga tgacagaaac tgaaatggag tataaggtta cagatggaat
    10561 atatgactat ataccaaacc aatgtttgac tgatttagtt tatgacaata tggtttttgt
    10621 tggttgccct aaaaatactc ctgaagaaga agaatttgca aaaaaattgt gtgatactct
    10681 tactagagaa gaaagattag gtgtagcaac atcattacaa aatgataaat ctattgtaga
    10741 aagttatata cacaaggaaa tagttgacgg tgacaaagat aaaggactag ctggttctac
    10801 tgatgttgga gatgtaagtt atgtaatacc agtggctcaa tttgcaatgg cagcttggcc
    10861 tgttggaatt gcaagtcata catggcagtc atgttcatca gcaggttcaa atataggatt
    10921 ttctgctatg ataaattcag ctaaagtact ggcttgtagt gcttatgatg tatttatgaa
    10981 tactaaaatt attgatgaag caaaaattga atttgataaa tcattagatg gacaaaaatt
    11041 taaaccattg gtataatttt ggtaataaaa tatttctggg gggaatagct atgagagaaa
    11101 aagttgaaaa agtgcttgag gaaaagataa aaccagtatt acaaagagat ggtggagatg
    11161 tagagcttat agatgtaaat gaaaatggtg tggttttagt tagactacaa ggggcatgta
    11221 gtgggtgtcc aggagcgact atgacaatca aagctattat tgaaaatgtt ttggtgagtg
    11281 aagttccagg agtaactcaa gtattaggtg tttaatagtt agcgtttatt aaatgtatgt
    11341 tatacataag tatttaataa agaagtaaaa aaaatagtga agcaatatat atgaaaaagt
    11401 atgataaaga taataaaaca ttatagaaat aatgaaatat ttcatatgta tatgaagaaa
    11461 taagaaaaaa tagtgaagca atatatatat gaaaaagtat gataaagata ataaaacatt
    11521 tatagaaata atggaatatt tcatatatac atatgaaaaa atagtaaaac aatatatata
    11581 tgaaaaagtg tgagaaaata ataaaatact atagaaacaa ttaaatattc tatataggta
    11641 ttaagaaaca tgaaagaaat aatggagtat tatagataaa tatgtaaaag tgtgaaagaa
    11701 ggatgtagat aaatttgtct tatacaaatt tacctacagt atttcttaca cactttttat
    11761 tttactatcc aaccatacca ataaacatac ctataaagta tggaactaac catatagtcc
    11821 atacaactat actaaactta tgaaagaatt ttttcttttc ctcatcatct ttgtatagga
    11881 caaaagtagc ccaacttgca tgaaaaagca taagcacaat tgcaagaaga cctgtaatac
    11941 tatgaaggtt ttgtgataaa gcagatttta cttcaaaagt atggctgttg gctatgttac
    12001 tcattgtaaa tgttcctaaa gtatcaaata taaacccaag ccaaaatata attacatgtt
    12061 tcttttttaa aatttttgcc tttctttctc caaatacacc tatagtgtag aaaattaaag
    12121 ctgatgttat aaatactatt gcaagtatta atttataatt cataattatc tctcctttac
    12181 ttttgccaat atatttctaa gattttttac taagtcactc aattcgtttt catcacaatt
    12241 tttaaaaata tcatcaaaac tttcttgcat tttaatttta aaatttgtgg caaattcaaa
    12301 tcctgttgtt gtaaacttaa cgtatgtatt tcttttatca ttagatttct tgaatttttc
    12361 tatatatcca atctgttcta atctgctgat tatgcctgaa accgtacctt tagctaaaga
    12421 catttcatca catagttgtg atattgtaag ttcttgatta tgagctacca gttttataac
    12481 tataatctgt tggtgtgtta atccattttc tttaaaattt tcttctactg tgtacatagt
    12541 tttagaataa agttctttta agagcatggc tattctaaaa cagtcataat tacttttcaa
    12601 taaaatcacc tcttaaatgg tttgcataca aactaatata atatactttt cttatatagt
    12661 caacctaaaa ttttataatt ttatagaaaa taatgaagaa tagaacataa aaattttatt
    12721 ttaggtataa ataaaataat ttgatgaaat tttaacaatt tttaaaaagt ttgtttacac
    12781 agttaataaa tgatgctaaa attaactcat agataaatat agaaaattta attttgttta
    12841 atgataatgg aaccacgaag gttttaacgt tactttacgt ttgcttttat gtggtttttt
    12901 tattgcaaca aaggggggtt ggggtttgtt aaaggatata ctgaaaaagt tgttgcagtt
    12961 tattttagta atgtttttat tatcttttat tgttttttac atggcaagac ttgctccagg
    13021 agacccatta atctcttact atggggatgg agtagaaaga atgagtacac aagaaaaaga
    13081 aaatgctatg aaaaaacttg gtttgaatga accaatatac agtcaatata taaaatggat
    13141 ttcaaatgca tcaaaagggg aatttggaat ttcatttaaa tataaacaaa atgttacttc
    13201 tgttataaat gatgtctata taaacactat aatattaggt ggttcagggt atatacttac
    13261 ctttgtgcta gccttattgt taggaatatt ttgtacatta catgaagata agctcattga
    13321 tagaatcata tgcaaattag gaacaataac caactgtatt ccatcatttt gggttgcctt
    13381 agtactcata cttatattta gtataaactt aagtatactt ccaagtagtg gtgcttattc
    13441 aatgggagaa gagtccagta tattaagtag gatatcacat ctaatcttac ctcttacagt
    13501 acttatatta agccatctat ggtattacac atatatgata agaaataaat tactagagga
    13561 aataagagaa gattatgtgc ttttatgtaa agcaaaaggt ttaaataata gaactattgt
    13621 ttttaagcat tgcctgagaa atataatgcc atcatatatt agtataatgg ccatttcgat
    13681 acctcatata ctaggaggaa cttatgtagt tgaaaaagtt ttttcttatc ctgggttagg
    13741 aacactttgt tttgaaagtg caaagtatca tgattataat atgttattag ttttatgctt
    13801 aataacaggg gctttagtag tatttgggaa tatgttagct caaattataa acaataaaat
    13861 agaccctaga atgaaatatg atagaggtga tactaatgaa gccacaatat agtgattttg
    13921 aaattgtagg agaaaattat attttagtag cagaagatga acatatagaa acaaagaaaa
    13981 ccttatctaa aaaaattaaa gaattaccat atatatcaat tattatttta tcaatcatag
    14041 tcattggaag tgtattctca tcttttataa tgacacatga accaacatac atggatttag
    14101 caagttctaa tttagctcca aataaaactt ttttatttgg aacagactca atgggtagag
    14161 atatatattc tatgatatgg tatggaggaa aaatatcttt atttatagga ctattttcca
    14221 ctgtaatatc aactgttata ggaattgttt atggtagtat aagtggttca gcatctgaac
    14281 tcgtagatga tgcaatgatg aggtttacag aaatcatact tagtatacct tcaatattaa
    14341 ttataatatt tgtacaagct atacttggaa attcaaatcc agtatctatg tccatagtaa
    14401 tagggataac aagttggatg aatatatcta agatagtgag aacagaagtt agacaaataa
    14461 gaaatagcga atacatacta gcagctaaga gcatgggagg aggattcttc tatgtattaa
    14521 agcaacattt acttccaaat tttgtggcat caataatgtt catggttgta acaaacatag
    14581 gcgcagctat tggtactgaa tcgacactta gctttttagg aattggttta ccaatagaaa
    14641 ttgtttcttg gggaagtatg ttatctttgt cagaggaagc attactttca aatagatggt
    14701 ggattatact gattccgggt atatttttgg ttaccacatt ggtgtgtata acaaatatag
    14761 gtaattatat aagaaagaat aataataaaa aatctagtaa tttataaatc agcatttgag
    14821 tactaaataa gcaaatactg aggtatttga aatatctatt taaataaatg gggtcaaagc
    14881 tttgaaaaat attttataaa aagcaaaagg gggagcatca tgaaattaaa gaagttaaaa
    14941 gttttaagtt tagtaatgat acttagtcta atggcaggtt gttctagtgg aggagacaaa
    15001 gataagaagg cagatacgcc taaagatgga aaggtacttg tttatggaag taatgactat
    15061 acaagtataa atcctgcact atatgaacat ggagaaataa attcattaat atttaatgga
    15121 ttaacagctc atgatgaaaa taataaggtg gttccttgtc tagcaaagga ctggaaattt
    15181 gatgaggcaa caaatacata tacttttaat ttaagagatg atgttaagtg gcatgatgga
    15241 gagaaattta cggcaaatga tgttaagttt acaatagaaa ctataatgaa tccagataat
    15301 gcttctgaga tagcatcaaa ttatgaagat ataacaaaaa ttgatgtggt taatgataat
    15361 actataaaaa ttactttaaa agctccaaat acagcaatgc ttgattattt aacagtagga
    15421 gtattaccaa aacatgtttt ggaaggtaag gatatagcta ctgatgaatt taatcaaaaa
    15481 ccaataggta ctggaccatt taaacttgaa aaatgggaca aaggtcaaag tataacactt
    15541 gttaagaata gtgattattt tgtaaaagaa ccaggattag ataaagtagt ctttaagatt
    15601 gttccagatg ataaggctaa ggcaatgcaa ttaaaatcag gagaattaga cttagctcaa
    15661 ataactccta aagatatgtc taactttgaa aaagacgaaa agaattttaa agtaaatata
    15721 atgaaaacag ccgactatag aggtatatta tacaatttta attctaaatt ctttaaagat
    15781 aaaaaagcta aaggattacc aaatgcttta agctatgcaa tagatagaaa agcaatagtt
    15841 gacagtgtat tattaggaca tggtgtacca gcatattcac ctttacaaat gggaccatat
    15901 aataatcctg atatagaaaa gtttgaatat aatccagaaa aagctaaaca agaaattgaa
    15961 aagctaggtt ggaaattagg ttctgatgga atctatgaaa aagaaggtac taagttagct
    16021 ttcgagataa cagcaggtga gagtgaccaa gttagagttg atatggctaa aatatgtgca
    16081 caacagttaa aagaaattgg tgttgatgct aaagctgttg ttgtaactga aacagattgg
    16141 gctaatcaag atgctcattt aataggttgg ggaagtccat ttgacccaga tgaccataca
    16201 tataaagtat ttggaacaga taaaggtgca aattacagtg cttactctaa tcctactata
    16261 gataaaatac ttcaaaaagc aagagaaaca gaagataaag atgaaaaatt aaagttatat
    16321 aaacaattcc aagtagaaat gactaaggat atgccttata catttattgc ttatatagat
    16381 gcaatatatg ttggaaaacc aaacataaaa ggattaacac ctgatacagt tttaggtcat
    16441 catggtgtag gtatattctg gaacatagct gactggacaa ttgaataata ctgaaattaa
    16501 ttagaaacct tagggataaa aatatatccc taaggtttga atagcatagg ggttgatggt
    16561 ttttggaaca tttattggag gttaataatc tatctgtaag ttttaaagta gaagaaggag
    16621 aagttcaggc tgtaagaaat gtatctttta atctaaaaaa aggtgagaca ttggctatag
    16681 ttggagaatc tggttgtggg aaatctgtac tgtgtaagag tttgatgagg atactcccat
    16741 acaatggata tattaaaaat ggagaggttt tacttaaatc aagtgatttg gttaagaagt
    16801 ctgaaaaaga gatggaagat ataagaggaa agaatatatc aatgatattt caagacccta
    16861 tgacatctct caatccaact atatcaatag gaaagcaaat tgcagaagct gtaatcattc
    16921 atcaaggtat aagtaaaagt gaagcaaaaa agagagccat agagcttatt gaattggttg
    16981 gaattgataa tcctgagaaa agatttaaac aatttccaca tcatttttca ggtggaatga
    17041 gacaacgtat agtaatagct atagcacttg cctgtaatcc agatgtattg attgctgatg
    17101 aacctacaac tgctttagat gtaactatac aagctcaaat aattgattta ataaaggatt
    17161 tacaacataa aataggtttg tcaataatat ttataactca tgatttagga gtagttgcaa
    17221 ctatagctga tagaatagca gttatgtatg caggaaagat tgtagaaata ggaactgtag
    17281 aggatatatt ttatgaccct agacatccat atacttgggg gttgttgggt tcattaccta
    17341 ctttagattc acaggatgaa tatttatata atatacctgg aatgcctcca aatttattga
    17401 acccaccaaa aggagatgct tttgcaataa gaaataaaaa tgcacttaag atagattatg
    17461 aaaaagagcc accaatgttt aaaattaatg atacacatag tgcagcaact tggttattgc
    17521 acccagatgc acctgaagta gatgtaccag ttagagtaaa ttgtggaagg gtgatttcta
    17581 atgaatagag aaaaaatact ggaaataaaa aacttaaagc aatattttca tttggacaaa
    17641 agtactactg taaaagcagt tgatgatata agttttgata tatataaggg tgagatattt
    17701 ggattggtag gagagtcggg ttcaggaaaa tctacaactg gtaaaactat aataagacta
    17761 catgaatcta ctggtggaga agtaatttac aaaggaaatt gcatatcaga taaaaaaacg
    17821 tacaaattta taaaaaaaga tgtaaacaaa agtatgcaga ttatttttca agattctact
    17881 tcatcattaa atccacgtat gacaattgca gatataattt cagagccact aaaaattcaa
    17941 ggtatctgta aaaataaaac agatagaatg aataaagtat atgagatgct aaagctagta
    18001 ggacttgata gaagttatgc aaataagtat ccatctgatt tttcaggtgg acaaagacag
    18061 cgtattggca tagcacgagc tctttcagta gacccagaat ttataatagc tgatgagcct
    18121 atagcttctt tggatgtgtc tatacaagca caaatagtaa atttatttaa aaaattgcaa
    18181 caagaaaaga atttaacttg tttatttata gctcatgatt tatctatggt gagacatatt
    18241 agtgaccgta taggagttat gtataatgga aaattagtag aattggcaga ttcaaatgaa
    18301 ttgtataata atcctattca tccatataca aaatcactac tttctgcaat tcctgttcct
    18361 gaccctagat atgctaagtc aagaaataga atagagtata attctaatgg atatgattgt
    18421 tcaaatgaaa agtcattaag ttggattgag gtttcagatg gacattttgt atattcttct
    18481 aaatcagaaa taaataaata tcaacaaaat ttaaaagttg tttagtataa aaatacttga
    18541 acaggtattt atttgaaata caaactaaaa ccctattttt ggagaaattt taaaactact
    18601 ccaaaatagg gttttttatt tctacttttc aaatataata aattcaaata taataaaatt
    18661 aaaaacaagt aaagtatgtt gttaagaaag tgctataaaa ttaaaaatag taatgtagaa
    18721 aaaatgagca ataatcttat tttttctaca ataattttct ataatttaaa gtaaggaaag
    18781 tttgataaat tataacctta gttaaagaat aagagtttat taaaaaataa agtgtgtata
    18841 ggggttttag ttaataaaat attataatgt aggggagctt gtaatgatat ataaaaaaga
    18901 taaagtaatg aaagttttag aatcatcata tagggaaaat gaaaaaggga ttacagctaa
    18961 ccaattatca gaaaagacag gaattgcaag aaacaatata agtacatatt taaaccaatt
    19021 attcaatgag ggtatggtta cgaagataaa aggaagacct gtgtattata tccctgcaaa
    19081 tcttaacctt aacaagaatg aattgcttga tacacaaagt gagttgcttg gcataaaaaa
    19141 aggaacaaaa gtagaaaaca aaataaaaaa agatagtttt gaaacactaa taggtaaaga
    19201 acatagttta agacctataa tagaaaaatg caaggtcgca atgctctatc ctacaaatgg
    19261 acttcatact attatctatg gagaaacagg agtaggaaag agtatgattg ctagatatat
    19321 gtacgattat tctatagata gtggaattag aaaaaataaa gctccttttg taacatttaa
    19381 ttgtgctgat tatgctaata atgcacaatt acttatggga catatttttg gagtagaaca
    19441 aggagcttat acaggtgcag aatcttccag aaaaggtctt ttggaaattg caaatggagg
    19501 gatactattt ttagatgaaa ttcatagact accagcagaa ggtcaagaaa tgttgtttac
    19561 ttttatagat aagggaacat ttaaaagact aggagatgca acgaaagaaa gagattctaa
    19621 ggtattaata atatgtgcta ctacagaaaa tccgacatca acactattag atacattcaa
    19681 tagaagaata ccaatgaaaa tagaaatacc atcactgaga gataggacaa tgattgaaag
    19741 aatggaatta gtcaaaaatt ttttcaagga agaatcaaaa agtatttcta tttcaataaa
    19801 agtacataaa gaaataatta aatcactatt actttatgat tgtaaaaata atgtaggtca
    19861 acttcaaaat gatataaaat tagctgttgc aaatggatac ctaagatata aaagagataa
    19921 taaagaacca gtttatatag agaaaaaata ttttgataaa tctataaatg aagtgagtga
    19981 tgattataga gagaagtcta ttaaagcaga tgaagttata acacatggaa tagaatattt
    20041 tgtatttacg tcattaggag aagaagaagt tattaactac aatgatataa atattattaa
    20101 tgcagtaaat actaatctta ataaatctgt agaaatgaat atagcagatg cttctctatt
    20161 taataatgtt aagtttaaaa gcatgtgtga atcaataaga caaattatta aagaagatga
    20221 agaaatagat ataagtgaat caatatttaa gtcaatggca atttatataa aaagcgtatt
    20281 ggataaaagt agctctacaa acaacataga tttgaatcaa attagaagaa ataacagaag
    20341 tgaatttaaa acagcactaa aaatagttgg aataattgaa aatgaatttg atttattttt
    20401 atctatagaa actgtagcat atattacatt attcatagta caagctaaac aagatgaaca
    20461 aaataaaata gtgaataaca ttaatattat agttgctatg catggagaaa ctacagcaag
    20521 taatggtaaa agcagttggt gatattattg gaaagtgcag tgctatttct ttcgatatga
    20581 agttagataa aagctatgat gaagttattg tagattttaa aaatttaatt aataatatag
    20641 acaataaaga tgtattacta tttacagata tgggttcatt aaactccttt gatgaaatta
    20701 taaagaaaga aaaaaagtgt gaagtaagag taattcctat ggtgacgaca ttaactgtgt
    20761 tagaagcagt tcagaaggcc aatatgggat tacctttaaa tgacgtatac aactctatca
    20821 ctaacacgag aaagtattat tttggaacta atgaaattca aaataaagaa aatctgtcta
    20881 aaacaattat tatagcatca catgtttcag aaggtgtaga taataaaaca agaaaaatat
    20941 tagaagaaaa gatgagtagg tacttagatg gaatagatat tatatctgtc ccatataaaa
    21001 cagaaaaaga tttaagttta aatattacaa aacttaaaga aagtagtaat attgttgcag
    21061 taatcaatga acaaagaatt aatattagag gaattgacta tattagcaaa aaagatatag
    21121 ataaggatga aaatatcaat aaattaaaaa atattataaa gataagtata ggttatgatg
    21181 atgtagttga aggtttaaag acatccttaa aaagctctaa ttataatagg atatttaaag
    21241 atataaaata tgtgtctgat gaattgtttt tagtatttaa tatagaaaag aaatacgata
    21301 aagtaattgg acttatgatg catttagctt tcatggtaga tgggttgatt ggaaatacta
    21361 gagaaattga aaagcttgat aaagaaaaaa cacttgatta tcacaagagc ctaagcaaaa
    21421 taaaagatat agtatctcaa ttagataaaa aatataatat agaaattaat gaaaaagagt
    21481 gctatcaaat attattaata ttagaatatg cagaaataat cgaaaaagat tac
//
LOCUS       DAEPOG010000057        21122 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEPOG010000057 DAEPOG010000000
VERSION     DAEPOG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.057"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            63..539
                     /locus_tag="KR432_001757"
     CDS             63..539
                     /locus_tag="KR432_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0640297.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            637..789
                     /locus_tag="KR432_001758"
     CDS             637..789
                     /locus_tag="KR432_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBH0640298.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(949..1137)
                     /locus_tag="KR432_001759"
     CDS             complement(949..1137)
                     /locus_tag="KR432_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH0640299.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            1498..1848
                     /locus_tag="KR432_001760"
     CDS             1498..1848
                     /locus_tag="KR432_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0640300.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            1867..3510
                     /locus_tag="KR432_001761"
     CDS             1867..3510
                     /locus_tag="KR432_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBH0640301.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            3591..5648
                     /gene="recG"
                     /locus_tag="KR432_001762"
     CDS             3591..5648
                     /gene="recG"
                     /locus_tag="KR432_001762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH0640302.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            5707..6261
                     /gene="rsmD"
                     /locus_tag="KR432_001763"
     CDS             5707..6261
                     /gene="rsmD"
                     /locus_tag="KR432_001763"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBH0640303.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            6263..6760
                     /gene="coaD"
                     /locus_tag="KR432_001764"
     CDS             6263..6760
                     /gene="coaD"
                     /locus_tag="KR432_001764"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH0640304.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            6764..7288
                     /locus_tag="KR432_001765"
     CDS             6764..7288
                     /locus_tag="KR432_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640305.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            7632..9551
                     /locus_tag="KR432_001766"
     CDS             7632..9551
                     /locus_tag="KR432_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0640306.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            9564..9851
                     /locus_tag="KR432_001767"
     CDS             9564..9851
                     /locus_tag="KR432_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0640307.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            9984..11243
                     /locus_tag="KR432_001768"
     CDS             9984..11243
                     /locus_tag="KR432_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBH0640308.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            11246..11986
                     /locus_tag="KR432_001769"
     CDS             11246..11986
                     /locus_tag="KR432_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0640309.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            12309..13619
                     /locus_tag="KR432_001770"
     CDS             12309..13619
                     /locus_tag="KR432_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0640310.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            13717..14634
                     /locus_tag="KR432_001771"
     CDS             13717..14634
                     /locus_tag="KR432_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0640311.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            14650..15459
                     /locus_tag="KR432_001772"
     CDS             14650..15459
                     /locus_tag="KR432_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0640312.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            15516..17264
                     /locus_tag="KR432_001773"
     CDS             15516..17264
                     /locus_tag="KR432_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBH0640313.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            17527..18549
                     /locus_tag="KR432_001774"
     CDS             17527..18549
                     /locus_tag="KR432_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBH0640314.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            18554..20860
                     /locus_tag="KR432_001775"
     CDS             18554..20860
                     /locus_tag="KR432_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0640315.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            20832..>21122
                     /locus_tag="KR432_001776"
     CDS             20832..>21122
                     /locus_tag="KR432_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640316.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWS"
BASE COUNT         8362 a         2213 c         3851 g         6696 t
ORIGIN      
        1 aaataaaatt tatagactat ctttactaaa atatagaagg tctatagcct aggaggaact
       61 gtatgaattt aaaaagaagc aatgaaaaac gagaagagta cagaagaatg tattcagata
      121 aagaaagttt tttaagtttg attcaaaatt ttacaagtgt ctcaatagca aaagaattaa
      181 ctcttaaaaa tatagagtta gaaacatctt ttatatgtga atataaagga aaagaagttg
      241 atataattta taaggtattt tctaaaagtg gcaaagtatc acattatata gtgttagaat
      301 ttcaaacaga aatggatact gaaattgtcc caagattgaa atcgtataga gaacaaattt
      361 ggaaaagttt tattatgaaa aaaagccttg aagaaataga agataaaaat tttaaacttc
      421 ctaaagttat accagtggtt ttatatagtg gacctgaaag gttaagtaga aaaaaaggaa
      481 ttttggatat tgtagaatct atatcggagt tcagtaataa ttctagtatg aaaaattaaa
      541 attacataat gtgtttattg gtctaaaatg gaaaactgtc gcataaactt gaatatgaaa
      601 atatttatag atacaggttc tatgtggagg tgttccatga gaaataacaa gtctaaggta
      661 ttgctaggaa tactttgtct agcaacagga atggctgtaa ttctgtcaat gttgcttcct
      721 ggctggattt ggtcagcact taccgcatta atacttattg gatgtggagc attattattt
      781 ttttgttaaa atagttttaa ctataaagtg tggggtgagt attgatgaaa gtagtaacaa
      841 taaaattacc aaaatggatt tcaagtataa tcctaaaatt tatgagaaag agtaaaaatg
      901 attaaattaa aaagcctact tacggaagta ggcttttaaa cgtggttact aagctctttc
      961 aattttacca gaacgtaaac atctagtaca aacttttact ctcttaggag ctccatctat
     1021 aatcgctctt acgcttctta agttagctga ccatgttctt ttattatgtt tatttgagtg
     1081 tgatacttgg tttccagaaa ccttaccttt atcacatacg ctacatactt ttgccatcta
     1141 cgcacacctc cttaaaatga taactattta aagactaaaa acatatttat attatcataa
     1201 ataacaaaaa tttgcaatag aaatatatat ttgccttaca catattgaag aatttcttat
     1261 tatcatataa aattatatat ataattttat ataaagtaaa tatatttgga aaataattat
     1321 tatttttctt tgaatatata tactatagaa gaaattttat ccaaaaactt ttctaggttt
     1381 aaattatacc atatttatag tatgatatga ttataatttt atataataaa taagtaaaaa
     1441 tagtaaaaaa taaattaaga aatgttttta ataaaaataa gcttaagggg gtcaaccatg
     1501 agtgcaaagg taacgaatca atatggaaat atagaaatag ataatcaagt aatagctcaa
     1561 gtcgtatata gagctgcaat ggaaagttat ggcttagtag gtttagcata taagtctaaa
     1621 ggaatagtag agctattaaa aggcgagaac gcaactaaag gtgtaagagt tgaggaagta
     1681 gaagaagatg ccatagcaat cgaactttat gttataatac aatatggtac aaatatatct
     1741 actgttgcca ataatataat agatagagta aagtatgtag ttgaaaaaat gactggcgta
     1801 agagttacta gaattgatat taatgtacaa ggaataagag ttaagtaatt tcataggagg
     1861 aaacaaatga ttcaatatat agatggtaaa aggttaaggg agatgtttat ttcaggtgca
     1921 aacaatctcc aaaacaataa agagttagtt gacaagttaa atgtattccc tgtaccagat
     1981 ggagatacag gaactaatat gtcattaaca atatcttatg ccttaaaaga attagcaaaa
     2041 gttgaaaatg ataatatatc agatatcgga aaagcattat ctaaaggttc tttaatggga
     2101 gctagaggaa actcaggagt tatactttca caaataatca gaggtatagc taaatctata
     2161 gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag cttttaaaaa tggttcagac
     2221 acagcatata aagctgtaat aaaaccaatt gaaggaacta tacttactgt agttagagaa
     2281 agtggagagt ttgcaataaa aactgcgaaa aaagagaaag atgtagtaaa gtttttaagt
     2341 atgcttgtga aggaatcaaa ttcttcatta gaaagaactc cagaccttct aaagaatcta
     2401 aaagaagctg gtgtagttga ctctggtgga aagggtcttg tacttatata tgaaggaatg
     2461 ttagcttcta taaaaggcga taacatagaa ataaaaaatg ttgacctaga tactaatatt
     2521 tcaacaagta tggattttgc aaagagtact actagcacag atgatataaa atattgctat
     2581 tgtacagaat ttattttaga aagttctaag gtagaagata ctaaaataag agacataatg
     2641 atggcttatg gtgatagtct agcagtagtt ggagatgatg gagtcataaa ggtacatgta
     2701 cacactaatg acccaggaaa tgtacttcaa gaagctctaa agtacggtca attattaact
     2761 ataaaaatag aaaatatgaa gttacaacat gaaaatacat tactggatgt agaagaaaag
     2821 aaagaaaatg atagtgaacc tttagaagaa gagaaagagt ttgggtttat agctacttct
     2881 atgggtgaag gtttggcaaa tatatttaaa gatttcggag ttgaccatat aatagaaggt
     2941 ggtcaaacta tgaatccaag tactgaagat tttatgaatg ctataaagca tataaatgct
     3001 aaaaatatat ttattttccc aaataacagc aatataataa tggctgctaa tcaggcaaaa
     3061 gaattgagtg ataaaaatat aatagttata cctacaaaaa atacgcctca aggatttgct
     3121 gcattagtaa cttttaatgg agaattaagc gaagatgaaa ataaagaagc tatgatgaat
     3181 gctttaaact cagttaaatc aggtcaagta acttttgctg ttagggatac agtgatgaat
     3241 gaaattgatg ttaaagaagg taatataatt ggaattgcag aaggaaattt attatcagca
     3301 ggagattatg tggatgaagt aacatcaaac cttattgaaa agttggttga tgaagatact
     3361 gctataataa cattattctt tggtgaagat gttacagaat ctcaagcaaa tgaactacgt
     3421 acttctttag aagaaaagtt tgaagatgta gatgtagagt tatattatgg aggacaacct
     3481 ctttattatt acttaatttc agttgaataa tgattataag aacctgtatg accaagtagc
     3541 ttttataaaa ttggttgtac aggatttttg tgtagtaggt gatatatttg ttggatttat
     3601 ataaagatgt tcaatatgtt aagggaatag gacctaaaaa agcagataaa ttaaataaac
     3661 tgggtatatt tacactaaaa gacctattgt attattttcc aagacaattt gaagatagaa
     3721 ataatctaaa gaaaatagca caattagaag atggtgagaa agttacgata aaagcagtaa
     3781 tatcaagtat aaatacattt agcccaaaag aaggtatgac tttaacaaag attgatgtaa
     3841 aagatgagac aggctcggct aaattagtgt tttttaataa aagttatata aaaaatactt
     3901 ttcgtccagg agattcaata ttagtttttg ggaaggttaa gaaaaaattt aataacttag
     3961 aacttacatc atgtgaatta gaatatctta ctaattcacc taagaataca tgtagattta
     4021 tgcctgtgta ccagcttaca tacggtgtta caaataaaga aattatgagt ataattagaa
     4081 cagtacttga agataaagaa ttaattatac aagaatatat gccacaaaga attatagaaa
     4141 aatataggct atgtagtata gattttgcag ttagaaacat acattcacca agtagtaagg
     4201 aatcattaaa aatagcttta tatagaattg tatttgaaga attacttata ctacaactag
     4261 ggttgtttgt atttaaaagt ggcagaaata aagaagatgg aataaagttt gaaacaagta
     4321 aagacttaaa aaaaataata agtgctttac catttaaact gactaaagct caaaacaggg
     4381 cattagatga aattatacaa gatatgaatt tagagaaaat aatgaataga ttggtacaag
     4441 gtgatgtagg gagtggtaaa acagtagtag ctctcttagc acttgcaaat tgtgttttaa
     4501 atggttatca gggcgctttg atggcaccta cagagatatt agcagggcaa cattacattt
     4561 cacttactga gtctctaaaa gattttggaa taaatgtagg tctattaata ggtagtctta
     4621 caaaaaaaca aaaggataca gtattggaac aaataaggaa taatgagatt gatatattaa
     4681 taggtacaca tgcactaatt gaagataagg ttgaatttaa taatattggt ttagttataa
     4741 cagatgaaca gcatagattt ggtgttatgc aaaggagcaa actttcattg aaaggagcaa
     4801 atcctgatat attagtaatg acagcaacac caataccaag aactttagca cttatactct
     4861 atggagattt agatatttct attatagatg aacttcctcc aggaagacag ccaatagaaa
     4921 caattgctat tgaaaaaagt aagcgagata gagcatataa taaccttgta agaagagagg
     4981 ttgaatcagg aagacaagtg tacattgtat gtcctttggt agaagaaagt gaagctatag
     5041 aagctaaatc agcagttgaa cttgtagagg aattaagagc tgaatatttc cacgatttaa
     5101 ggcttggatt gcttcatggt aaaatgaaat cgagtgaaaa agacgaagta atggaacgtt
     5161 ttaaaaataa agaaatagat attttagttt caacaacagt tatagaagtt ggtgtcaatg
     5221 tcccaaatgc aacattaatg ataatagaaa atgcagaaag atttggactt gcacaattac
     5281 atcagcttag agggcgagtt ggaagaggta gtcataaatc gtattgtgta ctaatttatg
     5341 attctaaaac agatgtatgt agacaaagaa tggctataat ggaagagacc aatgatggtt
     5401 ttaagatttc agagaaggat ttagaaataa gaggacctgg agaatttttt gggacaaggc
     5461 aacatggtct tccagaactt aaagtagcaa acttatttaa acatataaaa atattgaagt
     5521 tagcacaaca agaagctcga tatatattgg gtgaagataa taatctacaa ttgaaagaaa
     5581 acatggcact caaaaaagaa attatagata aatttaaaga tacattgaaa gaaatttcat
     5641 taaattaatt ttaaattatg ttaaaatatt actaatgtaa taatgtgctg aagggagatt
     5701 gtcatattga gagtaatttc aggaaaagcg agaggattaa aactaaatac tccaaaaaat
     5761 gaagatgtaa ggccaactac agatagagta aaagagtctc tatttaatat gataaattca
     5821 tatataatgg aaagtgaagt tctggattta tttgctggga ctggttcttt aggaatagag
     5881 tgcttatcta ggggtgccaa gtcatgtact tttgtagata tcagtaagga aagcatagat
     5941 attgttaagt ccaatgtaaa aaaggcaaga gttgaaagtg aaagtattat tttaaacctt
     6001 gattttaaga ctgcaataga taaacttaaa ttacaaaata gtaaatttga cataattttt
     6061 atggaccctc cgtattataa aaatatgttt atagaagcta ttgaaaaaat tgataattct
     6121 aatctattga acgaagatgg aataatagtt gtagaacatg atacgaatga tttatttcca
     6181 gataagatat gtaaattgga gaaaactaga gataagaaat atggaaatac tacattaact
     6241 ttttataaga tggaggcata atatggaaaa taaacctaga aaagctatat ttgcaggaag
     6301 ttttgaccca attactaatg gacatttaga tattatttgc agggcttcga agttatttga
     6361 cgaattacaa ataggagtat taaataatcc taataaaaag ggtctattta gctttgatga
     6421 aagagtaaaa cttatagaaa aaagtacaag ccatttaaat aatataaaag ttgtgacttt
     6481 tgatggttta ttaataaatt actgtcagga aaatgggatt ggagcattag ttagaggtgt
     6541 gagaagtggt gctgatgttg attatgaact tcagatggca catatgaata gagagcttaa
     6601 tccagatata gagactataa ttttaccaag ttgtacaaaa tattcattta taagttcatc
     6661 tttaataaaa gaggttttgc tctttgatgc tgatataaaa aatttggttc caaagattgt
     6721 tttggaagaa ttaaagaaaa aaactagtgg gggtaattga tatatgaaga tagatttaga
     6781 aatgattgaa ctatttgagc agttagagga gatatttaga agtgcttcta caatcccttt
     6841 ctcacataaa tgcacagtgg ataaagatga ggttttagca ataattaatg atataagaac
     6901 tttaatacca gaagaagtaa cacaggcagt atggattaat aaagaaagaa ataaaataat
     6961 aagtcaagct aagcaagatg ctacaaatat agttgaacaa gcgaaaaaag aagctgagag
     7021 aatacaacaa gaatatcaag ataatataga ggaattaaag aaaaattctg aagatgtagt
     7081 aaaggcatat gtagaatcca gcgaacctgt tgttcaagca gaccaaagag caaaagatat
     7141 agttgataga gctgagagaa tagccaatga gataagaatt gggtcaattg aatatgcaga
     7201 ggatgtgctt tctagtgttg agtataattt aagagaaata ttagaagaag taaaaagaga
     7261 taaagctgaa cttagaccta aaaaataaat tttaaataaa taaaaatgga gctatcacac
     7321 aaaaaattcc aattgatgtg tgatagctct cttttaatat agcaattttt tgttatataa
     7381 ttgtgctttt tagattactt atatcttatt ttgatattat aatgtagctt aatcttttat
     7441 ttgttgaaat caacttattt atttgtataa ataatatagg ttattttcaa atttttttaa
     7501 taattttacc actgtcttct tttagattag ggttagactg tgcaaaatta taaaataata
     7561 ctaaatcatg tgcaattatt tggtatggca ctacatgtag aaaatataga taataaaatt
     7621 aaggaggtag tatgaaggaa agggctaaaa aaatattaga ttttattatt aaaaataaca
     7681 atgtaacatc acaagaacta caagaaaggt ttaatgtttc aaagagaact atttattacg
     7741 atattctagc tataaatgaa caactaggaa aaagtggtaa tataaaaaat gtaaaacaca
     7801 aatttatatt tgaaggtaat ttatgtgatg caaggaaaat aatttcaact gaagaagata
     7861 aatttttaga tagtgattac agaaaaactt ttatattaaa caagatatta cttggcgaaa
     7921 aaatatcaat agaaaaacta acaaatgaaa tgttactttc aaaaaacaca gtagtacaaa
     7981 caataactga tgtaaaaaaa taccttcaaa caatggggtt aagattagag tataagggaa
     8041 agtacaaaat tataggtgat gaatacgtta ttagagagct gtttctgata attgttcaag
     8101 aaaatgtact ggaaattaat tctataagta aggaagtaag tagtttcgat acaaaaggac
     8161 atataaaact tactgattat tctttattaa atctaactaa gtttgtggaa tttctaaata
     8221 aaaggataag agatgggaaa acattatact catataaata tctaaatgaa gctaaaaaaa
     8281 ttagttactt tagtaattgt aaagaacttt tatgtgaaga agccaatgaa aatgaacaag
     8341 cctatatatg cacttacata agttcacttc ctagcttaaa tagtgaagtt aaggaagatg
     8401 ttgttgaaga atatgtagat aaacttatag ataagtttga agtaaataca gcaataaaat
     8461 tagaaagtaa gcatgagttt aagaaaaata ttttaaggca cctccactct tcttacaaca
     8521 gaatacgatt taagtttcct attagaaatc caatgttaga tgagactaag tataaacatg
     8581 agtcacttta taaaattata aaatcaatta tagaaaatga agaagagttt cctgtatttg
     8641 aggggataag agaagaagag ataggattta tagcagcgta ttttggaggg tatttaagag
     8701 ggtctaggga taatggccta agaagaaata aagtcttact agtttgtcct aatggtctta
     8761 tggtatcaaa gagtttggaa atacaactat ataaatacat accaaccata gaaatagtag
     8821 gtatagtgtc cattaagcaa ctaaaagaag taaatgtata ctacgattac ataattacaa
     8881 ctatagatat acaaaatgta aacaatgtaa ttgttgtaaa tcctctttta acatcatcag
     8941 atgttcaatt acttatgaac aaactaataa gtgtaaaaga aaacgagaag tatttcaatc
     9001 ttgaattaat aatacaagct ataagaaaaa atggagttat caataatgaa gaagcactaa
     9061 aagctgattt attaaacata atacacaaga tagatgaagg agagatgtat caaccaatgt
     9121 taaaggaatt aattaatgca gaaagggtca atataataaa aagtgttaga gattggaaag
     9181 aagcaataaa aatagcctca aagccattgt tagaagataa ctctatagaa gagttatata
     9241 tagaaaatat gataaagtca gttgaaaaat atggaccata tattgtttta gctgatagat
     9301 ttgctctacc acatgcaagt tctaaagaag gagtaaacaa gcttgctatg tctttactaa
     9361 tagtagaaga tgaagttgat ttattaggaa aaccagtaaa tatatttatg gtattagctg
     9421 ctgtagataa cactactcac attagagcat tagcatcact atcagagatg atgtacgagg
     9481 aagaaaatgt aaaactaata attaatggtg ataaaagttc aatactagaa cttataaata
     9541 agcaaaatta ggaggaatga aaaatgaaga tattagcagt atgtggtttt ggtgtaggtt
     9601 catcaatggt attaaaaatg acaatacaaa aggctctaaa gcagttaggt atagaatgtg
     9661 aagtagaaaa tacagatata aattcagcta gaggtgctga ttgtgactgt atattcacaa
     9721 gtcaagaatt gttaaatgaa ttaaaaggaa cttgtaatgt agatgtatat ccagttaaaa
     9781 agtacatgga tttagcagaa gtcacagaag ttgttcaaac ttttttaagt gataaaaatg
     9841 ataagaaata gtataagttt taaaaagtaa atttaagtta atcagaataa aataaaaact
     9901 tactaaattc aaaaatagta agaaagagag gagggattta gtggtagaaa acttaaacag
     9961 ttgacctatc actaaaaaat ataatgcttg attttatttc aaataatatt ttacgtaatc
    10021 catctatgtt aattggatta atagcagcat ttggtcttgt actacagaaa aagaaagcat
    10081 ctgatgttat aaaggggtca ctacttgcag ctttcggaat tataattatg gagactggaa
    10141 caggtatgtt agttagttca atagcaccaa ttaatggagc tttccaaagt ataagtggtg
    10201 gtgcagtaac aaaaggttta agtgatgtta cttttactgc aagttatggt ggaattatag
    10261 gtttagcaat gtttgcgggt ttagctattc acctaatgat agcaagattt actcctatta
    10321 agacaatctt cttaacaggt cacatgcttt attggttccc attcatattt gtagcttcag
    10381 gagttgaagc tggattaaat ggtggagtat taatagcttt tgcagcagta ttatcagcac
    10441 tctattggtc aataatgcct tggttattaa gaaaatatgt gtttgatgtt ataggagatg
    10501 attcttttac attaggtcat ccatctggtt gtttagcact tatatcagga tttgttgcta
    10561 aattagtagg aaataaagaa aaatctacag aagatataaa catacctcaa agtttatcat
    10621 tttttagaga aatatctata agtggtggaa tggcagtgtt tttaatgttt atagttgttg
    10681 ggatagtaat accaggtgcg tttaaagata gtaatgagtt tattggaacg tatgcaataa
    10741 agcaaggtct ttcttttgga gcaggtctta taataatgct tcatggggta agaatgctaa
    10801 taaatcaaat aatgccagca tttcaaggta tatctgaaaa agttgtgcca aatgcactac
    10861 ctgcttttga ctgtccaata ttatttaatt ataaaccaaa tgctgtatta ataggattct
    10921 tagttgctat gctaacatct acagctatga ttttaatatg taactacttt aatgtattta
    10981 atttgctact tataccactt gttataactt cattctttga gtgtggagca gcagcagtaa
    11041 taggagaatc acaaggtggt atacgtggag ctataatagg tacagctata gctgcaatat
    11101 ttatggtagt tttagtagga atatctgctg taatttactc aggaactatt caaaattgga
    11161 tactaatatt tggaggtaac gacctttcat tatttggtat aattagtaag tttataggtt
    11221 cactatttgg aggaatatta tagatatgaa atttaaatac atagatgaga taagaagatt
    11281 aattgatata atagaaaacg aagaaaaagt caatatggaa aaagcagtac aaatccttgt
    11341 tgaagcaata gaaagtaaaa atgctatatt ttcatttgga gcttcacatg caggaatatt
    11401 aacagaagaa ttattctata gagcaggtgg ctgtgttctt ataaatccag tatttgcaaa
    11461 atctctaatg ttagatacag cgcctataac tcacacaagt aagatggaaa gattagaagg
    11521 atatggtaca gctctagctg ataaaactcc aattaaaaaa ggagatgtag ttatagttca
    11581 ttcagtatct ggtagaaatc cagtaagtat agagtttgct atgaaagcta aggaaaataa
    11641 ggcaatagta atatgtataa ctaatttaag ttactcaaaa agtgtatctt caagacattc
    11701 ttcaggaaaa aaactttatg aagtatgcga tttagtgata gataatcatg gtcaaaaggg
    11761 agatgcctgt gtagagatag atggattaaa ccaaaaagtt agtccaactt ctactgtaat
    11821 agcaactact gtgatgaact ctattatagc tcaagctact caggaattag ttaataaagg
    11881 gctaaagaat cctccgattt tctatagtgc caatattgat ggtggagatg agttaaataa
    11941 gaagattttt gatgaatata aaagtgtaat tcattatgaa tattaaggtt aaaaaaatat
    12001 gttaatgaac tttgaagttt atcaacaaag aatattaata atatataata aatgctatca
    12061 tttaaaagct gtaggtaaaa atctacagct ttttattatt gtagattaaa aagatgcgta
    12121 attaaatttt aaattggaga aaataaattg gaaggtatca attataaata ttagaaacgt
    12181 tgtcatagaa aaaaactata ttaaaaaaaa gtacatagaa aaaactgatt agacaataaa
    12241 tacatgcttt gataatattt taaatggagt gaaaatgtgt agaattataa tgaaaaaaga
    12301 aaataaaaat gaaaaaagaa aataaaatgg agggaataaa agtgggtatg ttaaaaaaat
    12361 tatgttcact agctatggta gcaattatga ctacaacact tataacaggg tgttcaagtg
    12421 gaaatggaaa agattcaaaa aaggatggcg aaaaagaaaa actagttctt tggatgccac
    12481 cagtagaaaa taatatgaag gaagtttggg accctatttt agataaattt gaggaaaaaa
    12541 ataactgtga agtagattta cagataattc cttgggataa ttattctgaa aaatttgcaa
    12601 cagctataag tgctggcgaa ggtccagatg taggatatat gtatgcagaa atgtatccac
    12661 agtttataga aagtggagca gtagaagatt tgactaacta tgcaactaaa gaggataaag
    12721 agcaatctat ttatataaaa acatctgaaa tgatgggtgg aatgtatggt atgccattcc
    12781 aagcagcaaa tccaggggta ttatactata acaaggatat tttagagaaa ttaggagaga
    12841 aacctcctaa gacatgggaa gattttaaaa gaatttgtaa aaaagcaaca caagatactg
    12901 atggtgatgg aaagatagac caatggggat tagctcaggg atggggagct aaaacttttg
    12961 gtaacttaaa ctggaactgg tatccttatt tatggcaagc tggtggagat atttttaatg
    13021 atgatttaaa atcggttaaa tttaatgata agagtggttt agaagcagct aatttcttaa
    13081 aagaattaca agcatatgtg cctgaagatt ctttatctaa agatagtaat gaaatgatag
    13141 aaagcgtatt tggaccaggg aaagcagcat ttacaataat gttatcttct gctgcaacaa
    13201 gtgtatttga tgaatcattc ccggatttaa attgggggtt tgtaactgga ctagaagata
    13261 agaaagcagc aacttttgga gcagtagacc atttatcttt aatgtcatct gctaaaaata
    13321 aagaacttgc atacaagtta atacaacata tgttaagtgt tgaatcaatg acagaatttc
    13381 ataaagcaat accaagagca ccaataacaa aaggtgaacc atatcaaggt gatgaaagat
    13441 tcaaagaaat ggtggaaaat gacaaaaatg tatatagacc attagtagta ggacctcatg
    13501 gagttgaaat atatgaatat ttatggaaag aacttcaaac tatgatttca ggagataaaa
    13561 ctccaaaaca agcattagat gatgctgcaa aatattcaaa tgatttatta gcacaataaa
    13621 agtttaaagc tgtaatatgt tgtaaaggac tttacagatt aaaattagca ttgtatttat
    13681 aaatacaatg ctaatattta tattggagag gataaaatgg aaaacttagt tagagagttt
    13741 gaagaaaatt caaaaagaaa aaaaacaaaa ttaaatatta aaccatatat gtatatattg
    13801 ccattgggaa taattttagt ggcgttttat gtgataccaa taataatgtc aatatacttt
    13861 agttttacaa agtataatat tattagtcca gccactttta taggtttaga aaattataaa
    13921 aaattattta cagatgaaac attaaaagta tctattataa atactattaa atttactgtt
    13981 gttgtagtac cttgccaaac tatattatca ttgattttgg cagtttggat tacaggaaaa
    14041 ggaaatagta aaatagcttc atttgctaaa ggtgctatat ttataccagt attatcatct
    14101 atggttttga taggtatggt atggagggct ttattgaatg gagaaggttc tattatatat
    14161 caggtattag gtgcctttgg aatagaatca tctaaacttt taggagatag taagacagca
    14221 ttacctacac ttatgtttat atctatgtgg aaaaatatag ggtatttcat ggtaatttat
    14281 atttcagcta taatgaattt accaaaacac tgctatgaag tagcgaaggt tgatggggct
    14341 acaaaacttc aagaatttat aaaaataact gtacctttat tgaaaccaac tacaataatg
    14401 gttgtatttt tagggtcgat atggtcatta caagtatttg acttaattta tactgtgaca
    14461 ggtggaggtc caggtatatc tactatgagt atagttatgc atgcatttaa cttaaacttt
    14521 aaaaacttta attctggata tgcaatgact gtagcaaacg tattattttt gttaatagca
    14581 gtagtttcga ttttacaaaa taagcttgta aaacgagaca attcagattt ttagaaaggt
    14641 aggtataata tgaaaacagc aagaagatta ttaagtgatg gtttactttt atttatagcg
    14701 tgtgcaagtt tggtaccttt tatatacatg ttgataatat cattgaagat aacatataac
    14761 tcatatagtt tggatatatc tttttcaaca gtaactcttc aaaactatat agatatcttt
    14821 actaaaaaag gatttgcaca atattttttt aatacagcaa tagtatcttt ttcaggtgta
    14881 ttattaaatt tagtatttag tacattagca ggatattctt ttgcgaagat ggattttaaa
    14941 ggtagtgata agttattttt atttatgata atgactttaa ttataccttc ccaagttaca
    15001 atgataccac tatatataat aatgaaacac ttaggatgga taaatagtta tcttgcactt
    15061 attatgccaa taccaactgc atttggcgta tttataatga gacaagctat tttaggagta
    15121 ccaaaagagt tattagaatc agcaaagata gatggttgct cggattttag aattttaata
    15181 caaatagtat tgcctttaat caaaccagca ttaattacac tagcaatatt tacttttatg
    15241 ggagcatgga acgagtttat gtggcctctt atagctacaa ctaaagatgc aatgagaaca
    15301 ttaacagttg gattatctac tcttaagaca ttccagataa ctaattatgg acagatgatg
    15361 gcaggggcta caataacatt tttacctcca tttattttct atttgatttt acaaagtaaa
    15421 tttgttgaag gtgtgtcttt gtctggtata aaaggttaaa tagtctaatt taataacata
    15481 aaagaaactt atataaatat ggaggaattt aaactatgga aatttcatat gtactgaaag
    15541 attgcacatt caaacacaat aaatactcag ttatagaagc tgaaacttgg aaagagaagg
    15601 atttggatat tgtagtagct aaaggtgaaa aatttgcatt tcaaattatg ctaaaagcaa
    15661 cagaagaatt taattgtaca attgataaaa gcagtactat atcatggaaa ggactggaaa
    15721 atagagtaag attagcatta aatgtgccta atagtttaga aaataatttt agtataaata
    15781 tattaggata tgttcaagat gatacaaagg catttgtgaa tgatgctatt ttaagggata
    15841 aagatgtgtt agtagagcaa tatttgcctc aaacattttg gattgaaggt caagtaccag
    15901 aagattttga agaaaataac cttaatatag gtattgatat atttaaaagc tttggatatg
    15961 aagatgaaga aaaagtttgt acaattgatg ttcctgtaaa agtaagaaat gtcgtgctaa
    16021 agcctttaga taaaagcaag ttctttttag atttatggca acatccaagt tgtttagcta
    16081 gaatgtataa agttgagtta tggtctgata tacattttga aatagtcgat aactatttaa
    16141 aagaattagc atctttagga gaaaaagttg caacagttat agtttcagat tatccatggg
    16201 cagggcaaag ttgttataaa gtttataaaa atccatcaaa cctatatgaa tataacatgg
    16261 taagtgtatc aaaaggattg gatggaaaga ttaaatgtaa ctttgagagt atggataggt
    16321 atataagcat agctgataag tataaaatgt ctaaggagat agatttattt ggtctattag
    16381 gtaattggtg tgctggtgag tttggaaatc ctgtagaagg ttataaggac cctataagag
    16441 taagatattt tgatgaaaat gataaagtat ttaaatttat aaacaataca aatgatttaa
    16501 aagaatatat aggattagta ttaaatcatt taatagagtg tggattatgg gatagagtaa
    16561 ggattatagc tgatgaacca aataatccag aagtagtcaa agagtgtata gaatttataa
    16621 attctactgt aggtactcat caagttaaat ataaatcagc aactcacgac caaaactttt
    16681 tagatagagc taaagatgaa attgatgata tgtcaataaa tttaaaactt actatacaaa
    16741 attataaaga tatagaaaat cttaagaaaa aaatcaatga caagggtggg atattgactt
    16801 ggtttgtatg ctgttttcca gagaaaccaa atagtttttt aagttctcct tttgtagaaa
    16861 atagaataat aggatggtat acttattact ttggtcttga tggattttta agatgggact
    16921 ataatttatg gacagaagac ccttggaaag attcaagtta caaattccct atttggaaag
    16981 ctggagatat gttctttgtt tatcctggaa aagatttaaa gccagttaga agtgtcagaa
    17041 tggagaattt aagatttgga atacaggact ttgagttatt tacaatgtta gaaaaagaaa
    17101 agggcagaga gtatatagaa gttgagctta tgcaagaatt attaaataaa aaagaaaatg
    17161 ctgaaattaa aggttttggt gacatagaat taggatattc acttgataat tggggatatg
    17221 ataaagttaa aaaatatgta ttagagttgt tagacagagt ataaaaaaat ataagagtgt
    17281 ctcaaaatga actttttagt tcatgaggca ctctttttgt atgaaatcaa tatagtttca
    17341 ttgtttcaac aatgaaaaat aggattactt atattctaag atagcccttt agttttttag
    17401 ttgtattcaa tgttttaaaa ctaactataa acgctataaa gtttataaat gacaataatt
    17461 atatgtaaca tgatgtactt taatttgcta taatagggat tgaataatat tatatggaag
    17521 ataacattgt ttggggggat taatataaaa agaataggta ttattataat gtttttttta
    17581 agtatagctt ttattggatt ttatcttgca ataagaggta acagtgatac agttaagatg
    17641 caacgaggga taaatatagg taatgcacta gaatcaccta aggattttcc gtgggatgta
    17701 aaaatgtcaa ataagttttt tgatgatata aaagatgcag gttttgacac agtacgtata
    17761 ccagtaaggt tttctgatta tacgtcagat tctgataatt ttaaaattga tgaagaattt
    17821 tttaaaaaga ttgataaata tgtagattat gcattggata aagacttaat agttgtgttg
    17881 gatttacatc attttgaaga aattatgaaa gaaccaagag ttcataaaga agagttttta
    17941 aaaatatggc aacagatagc taataggtat caaaaatatg ataaaaaatt ggtatttgaa
    18001 ttattaaatg aacctaaaga aaatttatct tcacaattac taaacgaata cattgaagaa
    18061 gcaataaaga taattagaaa aactaaccct aaaagaacta ttatagtggg accatataat
    18121 ttctatcaaa ttgattattt gaatgaatta aatgttccaa aagattctaa tatagtagtt
    18181 tcttttcact attacgaacc aaatgatttt gcatttcaag gaaacattta tcataatgga
    18241 tttgagcact tgagcaatat tacttgggaa ggaacaaatg aacaaatgga ttatttaaaa
    18301 aaaaggtttg atattgtaga aaattgggca aataaaaata aggtaaaaat attcttgggt
    18361 gaatttggag taacaaaaga agcaccagaa gcttcaagaa gagcttggat aaaagctgta
    18421 agagaagaag ctgaaaaacg aaacttttca tgggcttatt gggagcttgc ttctggattt
    18481 ggtatataca atcaaattga agggacttgg gatagagaca tattaagtgc attaatagaa
    18541 aagaggtgat tttatgaaga aaaattcaaa aaagatactt ataactttaa ctattattac
    18601 taatatcgta tatatattgt ggagaatttt ttacacagta cctaaagaag aagggatgtt
    18661 tgccttaatc tgtgctataa tacttctttt tgtagagata atgggtatga tggaaatgtt
    18721 tgtacattat tatggtatgt caaatattga gtatcctgaa aaaccagtca taagtgaaga
    18781 actttatcca catgtagatg tattcattgc aacatataat gagtctgttg atttggtgag
    18841 aaaaactgta aatggatgta ttcatatgca gtatccagat aagaaaaagg tacatatata
    18901 catatgtgat gatggaaatc gtgaagaaat gcgtattcta gcagaaaaaa tgggtgtaaa
    18961 ttacataact agaactgaaa gagaaggagc aaaagctggg aatttaaata atgcaatgca
    19021 acatacaaac tctcctttga ttgctacgtt tgatgcagat atgataccaa tgcatgactt
    19081 tttaattgct acagtaccat actttttgaa aaatgagcaa gctaaaaaag atggagaaaa
    19141 agaggagtat gaaaaggttg gttttgtgca aactccacag agtttttata atccagactt
    19201 gttccaattt aatctgcact ctgagggaag gattccaaat gagcaagatt atttttacag
    19261 agatattcaa ttggctagaa ataggacaaa ttctgtaatt tatggtggta gtaatacagt
    19321 tatttcaagg gaagcacttg aagaagttga tggattttat acttattcta ttacagaaga
    19381 ttttgcaaca ggtatcttaa ttcaaagtaa ggggtataga tgttatgcta ttccagaagt
    19441 acatgcatca ggtttatctc ctacagattt aaaaagtctt attaagcaac gtgaaagatg
    19501 ggcacgtgga tgtatacaaa ctggtagacg tctaaatatt ttgtttagga gaggtttagg
    19561 tttttggcag aaaataagtt atatttcttc aattacttat tggtatgcaa gtataaaacg
    19621 ctttgtatat ataatggcac ctattttatt ttctgtgttt aatgtgattg tagtaaaatg
    19681 tacattactt caagtattgg tattttggtt gccaatgtat attttaagta gtttatctct
    19741 taaaatattt tctcaaaata taagaaatac tagatggacc aatatatatg aaactataat
    19801 gtttcagtct ttaatgccag cagtaatact tgaaacgttt gcaatatcta aaaataaatt
    19861 ttcagtaaca aataagaata aattagaaga aaatagaatg tataagtttt tacagggaat
    19921 accttatttt atttacatgg tcttatctat cataggaatt ttaaaaatgt ttgtagcaat
    19981 atttaaaatg agttcaatga catattcagt tgtattattt tggttgattg gaaacctttt
    20041 taaccttgtt atggctacat tatttatatc agggagacaa cagttaagaa agtctgagcg
    20101 ttatattgct gaaattgatt ttaagttaaa gcaaaactct tatgttcttt cttcaaaaac
    20161 gattgatata tcagaaaatg ggtttgcatt tttgctagaa aatccagaat atatttcccc
    20221 agaagaagag ttcgaggtag agtttagaga aaaatcagga aatgaaatgt atatagctaa
    20281 tatgaaagca aagattgtta atgttgtgga agtaaattca aagtggaaat atgcagctta
    20341 tattacacat attgaagata gtgaaataga taattggatg tgtattgttc acgatagaat
    20401 acctacttta ccgatgacaa tatctaatca actaggtttc tttgatgatt tgcaaattaa
    20461 tgtaaaaaaa cgtattgaaa agattagaac attatcacga agaagtccaa gaatcaatat
    20521 gaattttcag atggatataa aaaatacagg taaattaaga atagtaaatt ttaattatca
    20581 atatgtatta ttaaattttg agaataaaaa tatttatcct aaagaaatag cattggaaat
    20641 taatgaaaat attgtgttag aatgtgattt atgtgagggt aaaatcgatg aaagaggtct
    20701 cttatataaa gtaaataaca ttgacagcat aatgcaaaat ttctttttaa gagatgaaat
    20761 gatggattgg atattacaaa ataagacaat tcctgtttca aatcctagtg aaaagaagga
    20821 aaaaagtata gatgagtttg aacccatgga atatatttag gagagatgaa atgaagactt
    20881 taaaagtgtt tttttgttct gcaaaatatc ttattttggt gtttattttt tttataaata
    20941 ctacttttat aagttcagcg acaagtgata aaattttaga tttatccttt aaaaagatag
    21001 agactgattt aagttctagg attacatatg aagatacagg tgtaaaaata gaaacagata
    21061 gttctaaaag tgataaggaa cgttatttat atatttacca aaatataaaa gaaaattgga
    21121 gt
//
LOCUS       DAEPOG010000058        21054 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEPOG010000058 DAEPOG010000000
VERSION     DAEPOG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.058"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            824..1585
                     /locus_tag="KR432_001777"
     CDS             824..1585
                     /locus_tag="KR432_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0640317.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1650..2432
                     /locus_tag="KR432_001778"
                     /pseudo
     CDS             1650..2432
                     /locus_tag="KR432_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2699..3118
                     /locus_tag="KR432_001779"
     CDS             2699..3118
                     /locus_tag="KR432_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBH0640318.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQNGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(3221..3487)
                     /locus_tag="KR432_001780"
     CDS             complement(3221..3487)
                     /locus_tag="KR432_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBH0640319.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3714..4415
                     /locus_tag="KR432_001781"
     CDS             3714..4415
                     /locus_tag="KR432_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640320.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4497..5105
                     /locus_tag="KR432_001782"
     CDS             4497..5105
                     /locus_tag="KR432_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0640321.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5429..5893
                     /locus_tag="KR432_001783"
     CDS             5429..5893
                     /locus_tag="KR432_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBH0640322.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6530..7546
                     /gene="gap"
                     /locus_tag="KR432_001784"
     CDS             6530..7546
                     /gene="gap"
                     /locus_tag="KR432_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0640323.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7941..9110
                     /locus_tag="KR432_001785"
     CDS             7941..9110
                     /locus_tag="KR432_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640324.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9491..13744
                     /locus_tag="KR432_001786"
     CDS             9491..13744
                     /locus_tag="KR432_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0640325.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13893..14387
                     /locus_tag="KR432_001787"
     CDS             13893..14387
                     /locus_tag="KR432_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0640326.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14503..14880
                     /locus_tag="KR432_001788"
     CDS             14503..14880
                     /locus_tag="KR432_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH0640327.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            15086..15526
                     /locus_tag="KR432_001789"
     CDS             15086..15526
                     /locus_tag="KR432_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBH0640328.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            16157..17464
                     /locus_tag="KR432_001790"
     CDS             16157..17464
                     /locus_tag="KR432_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640329.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    17550..17812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17938..18741
                     /locus_tag="KR432_001791"
     CDS             17938..18741
                     /locus_tag="KR432_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0640330.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            18779..19390
                     /locus_tag="KR432_001792"
     CDS             18779..19390
                     /locus_tag="KR432_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0640331.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            19422..20093
                     /locus_tag="KR432_001793"
     CDS             19422..20093
                     /locus_tag="KR432_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0640332.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            20392..20754
                     /locus_tag="KR432_001794"
     CDS             20392..20754
                     /locus_tag="KR432_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBH0640333.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         8578 a         2015 c         3676 g         6785 t
ORIGIN      
        1 gtatagcaaa aaggctgtta tattaatgtt agaggttcag ggaaaatata taaagcaaag
       61 atatttacag ataatgtaga ttgcacagct ataagttaga taatagaatt atgtaatcaa
      121 gaatttacaa caaagtataa gataaggata atgctaaatt gccatacagg tgctagatgt
      181 gtaaatggat ttaattaata ctattgataa agtgcaacaa gcacataaac caattgatga
      241 gattattaaa actataagag atatagttga cataattaaa atattggaac ctatttataa
      301 ttttaaatca aatcaataat gattaagatt aaaataatat taaggtatca atgttataag
      361 agtacaatat aaggagtact atatagtact ccttacttat acatattagg caataggaaa
      421 tcttttcata gttaaatttt caaatatttt ttagggaact ttataattga gaattaaggg
      481 tatagtatgt gatgaactta ctaaatttat aattaggtta tataatggaa taatcatcaa
      541 tagttttact ttagggaaaa tatatgctta aggaagtata gcttactaat attattaaaa
      601 tgtatattta aaatgataaa aaaagaatta gacaagtatt tagtaaattg attatgtaaa
      661 attctatcaa gcaggaaccc tatgacttta gtcatgggag gttcagtagg taacttgttg
      721 aattaagttt atattattta aataaactac ttaatttaga taatataaaa agtcagaatt
      781 ttaagaatag agctatataa tcttagctat taggagaagt actatgatag gaatatattt
      841 tagtggaaca ggaaattcaa gatactgtgt agaaaaattt ttacaagaat atgatataac
      901 agcaaattct tattctattg aagataatga acttttacag cacatcaata atcatgagaa
      961 tattattttt agttatcctg tgcaatatag taatgtgcca aaaatattga aagattttat
     1021 aataaatcat tcaactttgt ggaatggtaa acgtgttttt attattgcaa ctatggctct
     1081 atttagtgga gatggctctg gtgtgttagc tagaatatta aaaaaatacg gtgctataac
     1141 tgttggtgga ttacatgtga agatgccaga tagtatagga gatgaaaagg tattaaaaca
     1201 ttctttagag tataataaaa agcttgtaat aaaagcagaa aagaaagtaa ctaaagctgc
     1261 taaaaaactg aaacatgggt caccaccaaa agaagggtta ggttttttga gtcatattgt
     1321 agggttattt ggacaacgat tgtattttat caataaaaca aatatgtata cagataaact
     1381 aaaaattaat actgaggaat gtattgtatg tggtaaatgt gttaatttat gtccaatgaa
     1441 gaatctagta attaaaaatt gtatgattgt tgcaaatggg caatgtacaa tgtgttatcg
     1501 ttgtgtaaat aattgtgcaa aaaaagcaat tacattgtta ggtaaaaaag ttgttgagca
     1561 gagcaatatt aaaaaatata tgtaagaaat ttaaagtttt attgaatgta aattgatgta
     1621 tattttatgt tggaaagaga ggtcaaataa tggaatacta catagttgat tcatttgcaa
     1681 ctaaactatt taaaggaaat cctgctggag tttgtgttct tgacagaata atacctttag
     1741 agttaatgca gaaaatagca gaagaaaaca atttaccaga aacagcattt gtagttaaag
     1801 gtaaaggaaa ttatgaatta cgatggttta ctcctaaagc agaaattgat ttatgtggtc
     1861 atgcaacttt agcagcagct tatgtaatat caaattttat tgatgttaat gtaaaaagga
     1921 tagatttctt tactcaaagt ggaaagcttg aagttacacg aaatggaaat ctatatgaaa
     1981 tgatatttcc agaaataatg cctattgaaa tagagttatc accataacaa gctaatttga
     2041 taggctgtgt accaagtgct gtctattctt caagagattt aatcttattg ctaaacagtg
     2101 aacaagaagt tataaattat aaacctaatt acgcacaatt gcgtaaattg acagactggt
     2161 taggtataat aatcacacca caaggaagta atacagattt tgtatcaaga tatttttgtc
     2221 cagaattaga ttcagaagac cctgttactg gttcatcaca ttgtaatttt attccatact
     2281 ggtctgaaaa gttgggaaaa cacaaaatgg tagctgctca actttctaat cgtggtggta
     2341 taattcagtg tgaagtgttg aaggataata ctgtaaaaat ttctggtgaa gcagtcttgt
     2401 ttatgcaggg aacaataaag attgatattt aaataagtat aataactaat ttattatgaa
     2461 aaataattaa ttttcttagt gttaacaata ataaagtaga gttaaaaagc ttgatttcac
     2521 ttaaatcaat atgatagatg ttgatactaa gattgacaag catgattaaa aaagataata
     2581 tttaatctat aaagataaaa acacagtctg gttggtagtc cagacgcagc aatagctgtc
     2641 agtaacctgc ctccttggtt gtccgttctt tagaaagtaa tttaaggagg aactagcgat
     2701 ggacaaaatt agtggaaaat taacagtctt atttgaagaa ccattttggg taggtatatt
     2761 tgaaagacaa aatggtaaaa aatatgaagc atgtagggta gtttttggag cagaacctaa
     2821 agaagtagaa gtgtatgaat ttatacttga aagatttttc tcattagatt ttggtagcat
     2881 taaattagaa aaaaatgtga ccaaagacaa cattggatac aaaagaatgc agagaaaagt
     2941 aaaaaaggaa caagaaaaag aaactatagg gactaaggct caaaatgctc ttaagcttca
     3001 atatgaagaa agaaaacagg atagaaaaaa cctagctaaa aatagaaaag aagaagaaaa
     3061 agaaagaata tttaatttga aacaagaaaa gagaaaagct aaacataagg gtcattaatt
     3121 ttaattaaat atctttttaa taattgctag aaaaaggtgt ataaagtttt taattttata
     3181 caccttaatt tattgaaaag aataaggttt tatatttcaa ttactttaaa atataaaaat
     3241 atactaataa aaattgtaaa aatagtataa atggtatacc aaatgtaaat ttaatgtgtt
     3301 tagttttatg tctaaaagaa tacattccaa tcatagaacc tatactacct ccaattactg
     3361 ctatagacat aagagtagct tcttttattc tccattcgtt tctaattgct cttttcttat
     3421 ctatgtacat tgaaaagaaa gcaatagaat taatcactat aaaatatatt agaataaaat
     3481 ttttcacagt cttgcctcca taaaaatata ttatacatta taaaacaaga tatttgtttt
     3541 ggaaagttaa atttgtaatt agtacatata aaatatctga taagaataaa tataatatta
     3601 attggaaaat ttaaaaaaat taaacaatta gcaaataggg ggtatattta taagacacaa
     3661 agtgataaaa tctatttaga aaataatgta aaataacagg aggataaagc aaaatgaaat
     3721 ttatagtaga aaaagaagtt tttgataaat tagaaaatgt atgctttggt gtagtagttg
     3781 caaaaggaat agataatact aaagaaatag aaagaataag caacctttta gatataagta
     3841 tagatagagt agaagattac ttcaaagata aaaaagtaaa ggaatcagaa gaaattatac
     3901 catatagaga ggcatttaga agtctaggca tgaacccaaa taaatttatg agttcgatag
     3961 aagcaatgac aacaagggtt gctaagaata agaaacttcc tcatataaat cctattgtag
     4021 atttaggaaa ctctatttct ttaaaatatt tacttccaat gggtgctcat gatatggatt
     4081 ttagaaatga tgatgtatat gttagatttt ctaaaaaagg ggataaattt gtaccatttg
     4141 gagaaactga tgttgaatta atggaagaag gagaattaat ttattcagtt ggagacatgg
     4201 taaaaactag aagatggata tggagacaag gagaggaagg aaaaattact aatagctcaa
     4261 aaaatatatt ttttcctata gatggtttta ctgatgctaa tttagataag gtaatgagtg
     4321 caagagaaga attagcaaaa ttgttaaagg aaatatttaa ttgtgaaatc aaagtaggtt
     4381 ttgtagataa agataatcct gaaatggaaa tataataagt tgttgaatac ttgttaagat
     4441 attatattta cttaatgatg ttaaaaaggt attaaaaaag aaaggtcgta gtaaatttga
     4501 aaaaaactat aataaagaat gtaaatccaa tgattgaaaa taagtttata ggactttttg
     4561 aaatagagta taaaaacaaa ttagatgaag ataaggtttg gatggtagca tcaagaaaaa
     4621 gtagtgagca attaaaaaat atttatttga agaatgaaga agatagtgta gatgcagttg
     4681 taatagttgg gcttcataaa agtagtaaaa aattaatttt aataagacaa tttagagtac
     4741 ctattaatgg atatatatac gagttaccag caggtcttgt tgatgaagga gagtctatag
     4801 atatttctgt tgaaagagaa cttagagaag aaacaggact tactctttta gaaattaaaa
     4861 aaaataaaag tattgataaa gtttatttat ctcctggaat gagtgatgaa tctgttgcct
     4921 ttgtttattg tatatgtgat ggtgatataa cagatgaatt tttagaacca gatgaagaga
     4981 ttgaagcgct tttaatttcg caagaagatg caaaagagat tttacaaagt aatcataaga
     5041 tagacacaaa agcatttttg attttacaaa tgtttgtgag tttaggagct aaattatttg
     5101 aatagatata tagaaaagag gttatataga agagtaaaat aataaagttt attttacttg
     5161 tataaattat aatctctttt tttatacaat aaagtataat tttgtgtatt tttaaataag
     5221 ttacttgact cttaaatttt cataatatgt tataaaccaa tttaagatac tacaataatt
     5281 aaaattccaa aattatttag catatttgca attaaaatga gaaaaggtaa aggctgaatg
     5341 attgaaaaat ttgatgtaaa atagtagatt agtttaatat tatagtttgt agggttaaaa
     5401 attagaaatt gtaaaggaga taaattctat gaaaaaatta gttattattt taattagtat
     5461 cattagtgtg gttagcttga cagcttgtaa aaaaagcaat gaaagtaaat ataataattt
     5521 aaacaacaaa gaatcaggac aaacaaccat tgaaatggac ttagataaaa attatgatac
     5581 ttcagaccca tttgttaatg cacgtttgtt ttgtgtgtct aatgatatag atgttttaga
     5641 cacagagatt tcttttaaaa tggatggaga tagtggtatt gtggaaataa aggacaataa
     5701 aacagatgaa actttatgga gtaatacatg gcatggaagc gttgataatg atacattcgc
     5761 aatttctctt tctaatatac aaaaagagaa agagtatgct atatgcttta ccggaattaa
     5821 aataaatcat gcagttgtaa aggttacttt tgaaagcaat ttagttaagg agaaagaaaa
     5881 accctcaaaa taaattccag cttgtctata acttgatatt cagtacataa aagcagttga
     5941 tatttatgat tgactgcttt ttcctatcaa aaaggatagg ttgtgcattt ttacttgaaa
     6001 tagtttatgc acatcaaaag ttggtgagca tgatattgga aatggtaagc atgaaatctt
     6061 gataacacaa ggaaataaat ttaagttttc aattaagtat atttccataa tttagttaac
     6121 aataagctaa ttattattta tattttgata tataaataat aattgtggca aattttaaaa
     6181 aatcatatat tttttgaact aaataaaaat ttgtttttat tattttaaat tagaggagat
     6241 tcttgaagat atattatttt taaaaataag tatagtttaa aaattcaaaa aaaagatggc
     6301 aaaatatgtc ccaagggaca ttgagtaaaa taaatgattt cagcttattt acactaaaaa
     6361 gtacatacta aatgaggggt gaaataaaga aaaatgatat actaattgtc ttttgataat
     6421 aaaaaaacat ggaatttgat tatttaatca agaaaaagta ttgaacaaat gatttattta
     6481 gtgtacaata atcatgtaaa aacatagtaa tggagggaga catttcataa tgaaaattaa
     6541 ggtaggaatg aacggatttg gaagaatagg aagagctgta ttaagaatag ctcaagaaga
     6601 attaggagat aatatagaaa ttgtagccat aaatgcaaga gcaacaacag aatctttggc
     6661 acatttattt acatatgatt catgctatgg aacttttaga ggtgaagtag aggcaaagga
     6721 tgaagacact ctagtagtaa actctaaaga gataaaaata ttaagatata gtgacccaga
     6781 agaattacca tggaaagaac tgggggttga tatagtaata gaatcaacag gattatttac
     6841 acaaagagaa aaggcagaaa aacatattaa agcaggagca aaaaaagtaa ttataacagc
     6901 tccaggaaaa aatgaagata taactatagt tatcggagtt aatgagaaag aatatgataa
     6961 tcaaaagcac aatataattt caaatgcatc ttgtactaca aattgtctag ctccttttgc
     7021 taaagtattg gacgaaaaat ttggtatagt aaaagggtta atgactacag tacattcata
     7081 tactaacgat caaagaatat tagataaaag ccataaagat ttgagaagag caagagcagc
     7141 ggctgaatct ataataccta ccactacagg agcagcaaaa gctgtgtcaa gagtactacc
     7201 acaattagaa ggaaaattaa atggtttttc acttagagta ccaacaccaa cagtttcact
     7261 tgttgattta gtgtgcgaac ttaaagaaaa cgttacatct gagcaagtta actctgtgtt
     7321 aaaagatgct gctgaaggag aattaaaagg tgttttagga tattgtgaca agccacttgt
     7381 ttctatagat tataaaggag attcaagatc atctattata gacgctctat ccacaatggt
     7441 tatagaagac aatatggtta aggtagtttc ttggtatgat aatgaatggg gatattcttc
     7501 aagaactgtt gacttagtaa aatatgtagc tgaaaaatta aaataatata tatttcttaa
     7561 agcaaatttt aaaaaaattt tttgaaaaaa ttatataaaa ctaaaaaaaa agattatgaa
     7621 aaatattgaa ggaaaaattt tttcataatc ttttttcttt catgcaaaaa gatacaagtt
     7681 acaacttata tttgcatatt aggtcgttag agaagtatat atatatttag taaggaatca
     7741 tactttttat aaaatgaaag ataagatgtc gaaaacttgc ataaatctga aaaattttta
     7801 ttattaaaat actgattttc attttaaaaa tgataaaaaa atgacaaata aagttgcaaa
     7861 tagtaatact tataagtata attaaaaaag agaaaaggtt ttgttaaact ttataacaaa
     7921 aaaagaaggg atgattcaaa atggagaagt taacagacag gagacaggtt aaggcagtaa
     7981 gcatggaaac cttcattttc ctaatattac tagtagttgg ttttgggtat gttggtagta
     8041 ttatgggagc aggaatgatg ttcaaagtaa taatgagtac agcacatgct ttactattgg
     8101 agacagtttt cttaattatg gctatggcag ttcttgcagg cgcattaagc gcactacttt
     8161 cagagtttgg agtaatagcg ttaattaata aaattttcgc tgtatttatg aagcctttat
     8221 atggactacc tggtgctagt atagcaggag ctataacaac ttatttatca gataatccag
     8281 ctattatacc ttttgctaaa gataaaacat tcacacagta ttttaaacaa taccaagttc
     8341 cagcattatg taacttagga acagcctttg gtatgggact tatcttaaca acatttatga
     8401 tttctcaagg tacagaatat gtacttccag cattaattgg taatttaggt gcaattatag
     8461 gtagcgtcat aagtgttaga ataatgttga catttactaa gaagttttat aaatacaatc
     8521 cggaagaaga taaagcaaca ggaacattag agaaaaagga tgaatttaga gaaatcagag
     8581 aaggaaatgt atttcaaaga gctttagatg caattcttga aggtggtaaa atgggggtag
     8641 atatgggaat ggctataatt cctggagtcc tagtagtatg tacattggtg atgttattaa
     8701 cttttggacc atctacagac cctgttacag gtcaagaagt ttatacagga gctgcatatg
     8761 aaggaataaa attacttcca gttattggag ataaacttgg atttatatta gaaccattat
     8821 ttggatttac atcaccagag gcaatagcat tccctataac ttcattggga gctgttggtg
     8881 cagcaatgtc tctagttcca gagtttataa agtcaggagc tataactcct aatgatatag
     8941 cagtatttac agctatgggt atgtgttgga gtggatattt aagtacacat attggtatga
     9001 tggatgcatt aaatgctaga cagttggcag gtaaagctat tctttctcat acaataggtg
     9061 gtttatgtgc aggagcagca gctcacttta tatttacact cgtaggataa acgtaatcag
     9121 ctaagataaa aaataatata caaaaaaatg gttgttgata tgtaataaga atttacatat
     9181 caacaaccat ttttttctga ctaaagataa tttataatac ataaacatta agtggatatt
     9241 agacttaagt tatatttatg taaaaagtaa caaaaatctg tagaaattta tgacaaaatt
     9301 tagtacacta actatatgtt cttatttaaa atttaaggtt agaattagga attacctagt
     9361 agagtaagct ttgattaata aaagaatcat aaaataaaaa tattagtcat aaagattata
     9421 aataacttac ttaaaataaa gccatagaac ttacaaaaat taaaaaagca tataaatatg
     9481 gaggggtaca atggatgaat tatttcatat aggaattgat gtaggttcta caacggtgaa
     9541 ggttgtggta cttaataata gtaataatat agttcataaa gaatatagaa gacattattc
     9601 tgatgtaaaa aaatcagtta aagaagtact aaatggtata tatgaaaaat tgggagatat
     9661 aaatactacc attataataa ctggttcagg tggaattgga atatcaaaaa aattaggtgt
     9721 aaaatttgtt caggaagtta tatctagtac aaaatcaata gagtattttc atcctgaaac
     9781 tgatgttgtc atagaacttg ggggtgaaga tgcaaagata acatatctaa gtggaggaat
     9841 agaccaaaga atgaatggaa tatgtgcagg tggcactgga gcattcatag accaaatggc
     9901 atcactttta aagacagatg ccagtggatt aaatgaactt gctaaagggt ataatgttat
     9961 atatcctata gcttcaagat gtggtgtttt tgctaaaaca gatattcaac ctcttataaa
    10021 tgatggagcc aatcagactg atatagcaat gagtattttc aatgcagttg tcgtacaaac
    10081 tgtgagtgtc ctttcttgtg gaagaaaaat agaaggaaat gttgcgtttt tgggtggtcc
    10141 attgtacttt ttatctgaac ttagagaagc atttaaaaga gtattggatt taaaagatga
    10201 aaacataatc ttcccagaaa atgcacaact ttatattgct attggagcag gattattatc
    10261 agtagatgaa gataatatac aactaaaatc tctaatagaa aaattggata gtataaagaa
    10321 tattgaagat ggagaagtaa atttattaga acctttattt aaagataaaa atgagtatga
    10381 agaattttca agaagacatg aaaaagaaaa aataagttat gtggacataa attcaataaa
    10441 aagcaattgt tatttaggaa ttgatgcagg ctcaactaca actaaagcag cacttattga
    10501 tgaggatgga agacttgtat attcttatta taatagcaat gaaggaaatc cattaaaaac
    10561 aaccattaaa gttataaatg aagtatatga tatattacca aaaaatatta aaatattgag
    10621 ttcaactgta actggatatg gagaaggtct tattaaaaaa gctcttaaaa tcgataacgg
    10681 agagatagag actatagcac attacaaagc tgctaaattt ttcaataaag atgtagattt
    10741 tatacttgat attggtggtc aagatatgaa gtgcctaaaa ataaaagatg gagtaattga
    10801 taatataata ttaaatgagg cttgttcatc aggttgtggc tcatttttag agacatttgc
    10861 aagttcttta tctatgacaa tagaagaatt tgcacatgaa ggtatttatt ctcaaaatcc
    10921 tgtagattta ggttcaagat gtactgtatt tatgaattct agagtaaaac aagctcaaaa
    10981 agaaggtgca agtgttggag atatatcagc agggctatct tattcagtaa taaaaaatgc
    11041 tttatttaaa gtaataaaga ttagagattt aaatgagatt ggtaataata ttgttgtaca
    11101 aggtggaacc ttttataatg atttggtgct tagaagcttt gaaaaactaa ttggaaaaaa
    11161 tgtaattaga cctaatattt caggaattat gggagctttt ggttcagcac ttatagctaa
    11221 agaaaaatat gaaagtggat acaaaacatc cttgttatct aaggaagagt tgaataatat
    11281 caagctagaa gcaagtgtaa ctagatgtaa aggttgttca aatcattgtc ttcttacaat
    11341 aaataaattt tctgataatg aaatttttat ttcaggtaat agatgtgaga agggtgaagc
    11401 tatttatggt gataaaaaaa taggattaga aaagaaaaaa ccaataaatt tatttaagta
    11461 caaatacaat agaattttta gatacaaacc attggaaaaa gaagaagcaa aaaatgggga
    11521 aatagggata ccaagagtat taaatatgta tgaagattat ccattttggt ttactttttt
    11581 caatgctatg ggctttagag ttgtactttc agataggtca tcaaaacagt tatatgaaac
    11641 tggtataact agtattgcat ctgaaacagt ttgttatcca ggaaaattag ttcatggaca
    11701 tatagaaaat ttaattgcaa aaggtataaa aaatatattt tatccaagtg taacaaatga
    11761 aaataaagag gattcaaatg cagataatta ttataattgt cctgttgtaa tatcatattc
    11821 tgaagtaata aaaaacaatg tagagaatat aagaaataag aacataaatt atataaatcc
    11881 atttataagt ctaaatgata aggaaaaatt gaagaaaaga ctatatgatg aattatcaag
    11941 acatttttct gatataaaaa taactaaaga agaagttaat catgcagttg ataaagctac
    12001 tgatgaacag aatttattta aaatggagat acaagcagca ggtgaaagag cacttaaaga
    12061 aataaaagaa agaaatatga aggggattgt gttatgtgga agaccatatc atatagaccc
    12121 agaaataaat catggaatgc cagagcttat aaattcatta gatatggcag tgcttactga
    12181 agactctata tgtcatttag ccaatgtaca aagaccactt agagttgtag accaatgggt
    12241 atatcattca agattgtata aagcagctag ttttgtaaga gataaaaact atttggaact
    12301 tgttcaactt aattcatttg gctgtggatt agatgcagta actacagacc aggttcaaga
    12361 aatacttaat gaaaaatcga agatatatac tattataaag attgacgaag gcaacaattt
    12421 aggagcagct aagataagga tgaggtcctt aaaagcagct atgtttgaga gagaaagcaa
    12481 aaatatagat gtaaaaaata taaaggtaaa caggatacag tatgctaaaa ataatcagat
    12541 aacgaacaaa catactatat tagcaccaca aatgtctcca atacatttcc aattccttga
    12601 aaaagctgtt aacttaagtg gttataatat agaaattcta aaagatactg atagtagtgt
    12661 aattgaagaa gggttaagat atgtaaataa tgatgcatgt tatccagcta taatagttgt
    12721 aggtcagcta ataacagcat taaaatctgg caaatatgat ttaaataata cttctgttac
    12781 tataactcag actggtggag gttgtagagc tacaaattat attggattct taagaaaggc
    12841 tatatatgat gctggattta aagatgttcc tgtcattgca cttagtgtaa atggaataga
    12901 agatagtggt ataatggata atatatcact aaaattaatc aatagactct ttatgtctgt
    12961 tgtttatggt gatttgctga tgaaagtact atatagagta agaccatatg aaaaagttac
    13021 tggaagtgca aatgctctat atgaaaaatg ggttgatata tgcaaatctt ctcttgttaa
    13081 agcaaaaatt tctgtattta ctagaaatat aaaagatata gtaaaagaat ttgataattt
    13141 agagatacta gatatcaaaa aaccaaaagt aggtttggtt ggagaaatat tggtgaaatt
    13201 ccatccaatt gcaaataata atttagtaga tatattagag cgagaaggtg ctgaagcagt
    13261 cgtgccagac cttacaaatt tcttcttaag ttgtgccttt aatactatat acaaacacac
    13321 tcatttagaa ggaagtagaa agagtagaat gataggagaa gcatttatat acataactgg
    13381 aatatatcaa agagtatata aaaaagcact tgataagagt gagagatttt atgctccagc
    13441 caatataaaa aatgttgcta aaagtacaga accagtagtg tcattaggta atcaaactgg
    13501 agaaggttgg cttttaacag gtgagatggt tgaattatta aatgaaggtg tagaaaacat
    13561 tatttgtatg caaccttttg gatgtttacc taatcacata attggtaagg gttctataaa
    13621 agaattaaag aggttgtata aaaatgcaaa tataatacct attgattatg acccttctgc
    13681 aagtgaggta aatcaactta acaggataaa acttatgctt tctaaggctt ttaaaaatat
    13741 ataagattta tattttgata tgttaaaagt taatgaattt aaaaatatac tctatattaa
    13801 gtatctataa aaaagagtta tcctattttt tagggtaact ctttattagt taaaaaatta
    13861 tatgtatact gatattaaag gggggaagta atatgagttt agtatatgat gtttataaat
    13921 caaaattagg aaatttgtac atattaagtg aagatggttt tatagttagt gtatacatag
    13981 gagatgaaaa atttaataat ttaaaaaatg aatgtattaa aaaaggaatt gatataaaga
    14041 taagtaagga agatttaaag gaagcaataa gccagataga tgattacttt aatggaaaaa
    14101 gaaaagtctt taatttaaat attcgtattg aagggactga atttcaaaaa tcagtttata
    14161 gggaaatgtt aaaaatacct tatggagaaa cattgtgtta ttcagatata gcaagaaata
    14221 taaacaatcc aaaatcagta agagcaatag gacaagcaag taaaagtaat aaaattccaa
    14281 taataatacc ttgtcataga gtagttggca aaaaaaatat tggtggatat atggggaatc
    14341 attctgattt gaaagagata ctattaaatt tagaaagaca gagttaactt agaaagaaat
    14401 gtttttattg ttaatatttg atataataat agtaaaaaga agtatttttt acaaaaacaa
    14461 tgaagaattt attttaattt acaaaaatga gggggaattt taatgtgtga taaagttata
    14521 gcatcaaaat atcatgagaa gggatttaat tgtgcagaat cagttataaa agcatataat
    14581 gaggaattta atacagatat accagtttgt ttaggaagtg gactgggttc agggtgtggg
    14641 gttgctagtt tatgtggtgc agtaaatgca tcaaatataa taattggata cgtaaaaggc
    14701 agaaaccacg aagatgaaag tactaaagct aaagtatatg ctaaagattt aactacaaca
    14761 gtaagaaaag agtatggttc agagttatgt atagacctaa aaaaagattt agttgcttgt
    14821 agagaaataa tggatttcgc atatgatagt ctaaaggaaa ctttaaaaaa agaattataa
    14881 gaaaataagc tatcaagtat aaattatttg atagcttatt tttatagtcg atactaaata
    14941 aatcaaatat attttagaat tattatttgt aaataaacag caaataattt taggattttt
    15001 atttaatata gtttttattt atatttattt ttgaatatgt tatactttgt ttaaataaat
    15061 atttgtaatg agggagatga gaagattgaa gaatctcaat ttacttatta ataaattata
    15121 ttcaaaaaat cataatgaag catataaaac ttttttattc ttagaaaatg aaagtttaaa
    15181 atcaaatatt acatattgtt tttttgactc attcctagaa atgattgata acgaaaactc
    15241 atatattcga acaaggggat tgttattaat ctctgcaaat gctcagtggg atattgataa
    15301 taaaattgaa ataaatatag atagtatact aagtcatatc gtagataaaa aaccttctgt
    15361 atcaagaatg tttattaaat ccatacctaa tattacaaag tataaagaaa atcttattaa
    15421 tcgcataaaa atggaattaa gtaatgcaga tataagtatt tataataata atatgaaacc
    15481 attagtagaa aaggatataa acgatacatt atctaatatt agttaaatat taatatagaa
    15541 attgaatatt atctagttac aattatttat aactaagata ataaaacaac aatggtgcta
    15601 atttatatgt ataaattaat tatgaaaaat ttatataagt aaaatatatt aacataaagg
    15661 taaaattttg tatactaaaa gggaatattt acagtttaat ggcagtttta aaataataaa
    15721 ttaaagctgt catttttatt taaaaatttt tagaaaaagt acaatagtgt taatgtgtga
    15781 attagataag gtgtatataa taatttagat gatatgatat aaaatatgtt gtaaattaaa
    15841 tgttttcttt gacaatatat acacaaggat gataatatct atatgaataa aagatagtgt
    15901 gtaatttgat aatatgtaat tataacatta tgatataatt acaataaagt atgtaataat
    15961 ctgtagaaat ttcaagctag ttatctaagt tttcggataa ctaaatctaa acggaataca
    16021 taatgtatta aataaatgat gatactaaaa tgatactatt tttatagaaa tttattgagg
    16081 tatataattg atgaaaataa tatattaaat tatataatat attgagtttt acataaaagc
    16141 gaaggtggga ataaaaatga aagaaattgc tgatgctata acatcagagt atttaagcta
    16201 tggtatcaat gaagatatta gtatctatga aggtagattt tgcatatatt tagataaaaa
    16261 atatagatgt aatgggaaaa tttattataa aatgacacct ccaatatcta taagttttaa
    16321 agcagacata ggttgtgttg aagagataga caatgaagat gacaatttag ttttggatta
    16381 tgataatgct attcttgaag ttcatggata caaaattatt tctataacaa taaatacttt
    16441 aagtgaattt agtgtagaag gttatatcaa tgatgactgc ataaaatcta agaactccta
    16501 tgttgaatat gtagatttta atataataaa cttagataaa atacctggaa aactaattaa
    16561 atacaatgat aaggtatacg caggtagaat tgaatttgat ataaatgatt atgttgtaac
    16621 tatagacaag cgatatgatt atagaaaaga attaaaatca gaattaaagt ctaaatctgg
    16681 agcaataata actcatatag gtaggattag aagaaaagat ggtagaattt ttagaacaaa
    16741 taatacaata aatcttttag atagaatatc aacagctttg agttttatgt gtggaagata
    16801 tgtagggttt tgtttggcca aagggtatag aagtggtaat gaagtttata gaatatggaa
    16861 tgaaaatcaa atatctcctt ttagatatgt accaacatgg tcagatacac tttcaaatta
    16921 tcataatatg gaaaaatata taagccttat gtgtaaaaag ctagaagatt tttattatgg
    16981 ttctgctata aagagtgttg ttgattggta catagaatct cttggaagtg ctaccatgga
    17041 aaacaatata atatctgtac aaattgcact tgaaactttg tcatatgtaa ttctagtgga
    17101 acaaagtaag atacttacag atgaagcatt tgactgtaat ttggcatcta aaaatataag
    17161 actcttactt gatacatgta agataccata tggtaaacat gaattaaata tatttgataa
    17221 tataataaaa aataaatttg atgatggagt ggatttagtt atttatttaa gaaatagcat
    17281 agttcatcca agtagaaaga ctcatagagc tgtgttagag gttgaagata tttggaatat
    17341 aatttcaata ggaacgagat atattgaact tgttttactc tttattttag gttatagagg
    17401 agaatattca aacagattag tagaaaggtg ttatggtgaa gtggaagttg taccttggaa
    17461 ctagattttt atatttattt taaaatatat attgacatat attgccttat gctataaaat
    17521 caatatcata ataataattc agaataacat taaatgctaa gaaaagaaat agtaaaggta
    17581 gttaatctta aacagagagt ttccgattgg tgagaggagc aatagatttt actttgaaca
    17641 catctttgag caggactctg aaagagtaga aatactttat taggagattt ccggaatcct
    17701 tgcacccgtt atagtgctag agtataataa ttttattgta cttaaagagg ttagtgtcgt
    17761 gaggcattaa tgaacagagg tggaaccacg agagttaact cccgtcctct agcagaagct
    17821 agaggcggga gttttttaat ataatttttt aagcgcgcag agaattatat tgcaatagat
    17881 acacttttta aaaaataatc aaaatatttt aaaaattaaa aggcgaggga gaatattatg
    17941 aaaaatatat taaaaaaagt aggaatattt acaattatgt taggtttatt aggtggagtt
    18001 gtaggttgtt caaaaccaga taatgaaaaa gataaggatg cttcaaagga atctaaaaaa
    18061 gaagtagttg tagggtttga taatacgttt gtaccaatgg gatttttaga tgaaaaggga
    18121 gatacagtag gatttgacgt agacttagct aaagaaacat ttaaaagact tggaatggaa
    18181 gttaagtttc agcctataga ttggtcaatg aaggaaactg aattaaatga ttcaaaaact
    18241 gtagatgtat tatggaatgg atattctata acagacgaaa gaaagaaaat agtatcatat
    18301 acagaaccat atttacaaaa taaacagata atagttactc taagtgattc aaaaataaat
    18361 tcaaaagctg atttaaaaga taaagaagtt ggaacacaac aaggttcaac agcacttgat
    18421 gcagtggaaa aggataaaga ttttatgaat agcttaaaag gtggagcacc agttctttat
    18481 gatacttatg ataaagcact tagagattta gagataggta gaactagtgc agttgttggt
    18541 gatgaagttt taattagata ttatatggga caaaagggtg aagacaaata caaggtatta
    18601 aaagacgatt ttggattaga agattatgtt gtagcaactt ctaaagaaaa tccagaactt
    18661 tgtgagaaaa taaataaaac attaaaagaa atgaaaaaag atggaacttt tgacaaaatt
    18721 tatgataagt ggtttaaata gaagataagt tagctgttaa gaagggggca ctaaaggcat
    18781 gttacaaggt ttagagatag ttatagcaat gttttgtata acattgatag tatcaattcc
    18841 attggggatt ggagttgcat ttttaagatt atcaaagaat aaattagtaa gtggtattac
    18901 acaatgttat atattgataa tgagaggtac acctttactt ttgcaaatga tagtcatatt
    18961 ttacggatta cctcttttag gaattgtatt tgacagattt acagctggag tagtagcttt
    19021 cttcttaaat tatgcagcat attttgcaga aatatttaga ggtggaatac agtcaatcga
    19081 tagaggacaa tatgaagctt caaaagtact aggatttgat aaatttacta tgtataagag
    19141 agtaatcttt ccacaagttt ttaaaagaat actagcacct atttcaaatg aagtaatcac
    19201 attagtaaaa gatacatctt tagtatatat attaggatta aatgatattt taagaatatc
    19261 tcaaatagca atgaacaggg aagcaagtct gttaccatta tttgaggcag gagcgatata
    19321 tcttatattt gtagctatac ttactaaggg atttgaatta cttgaaaaaa aatattctta
    19381 ttatagataa agttacaata ttataggagg aggtttttta tatgttgaaa atcaaaaact
    19441 taaataaatc atttaaaaaa aatagagtac ttaaagatat ttcttttgag ttagaagaag
    19501 gtcagattgg agttttactt ggtaagtctg gtgcaggaaa gaccacaata ttaagatgta
    19561 taaatggtct tgaagaattt gatagtggag agattataat agataatgag gtaataaaaa
    19621 ataagagaga tatggcaaaa atacgaggaa aaataggtat ggtttttcaa aatttcaact
    19681 tatttcctca tatgacagtc cttgaaaata taattgaatc cccagttaat gtttttaaag
    19741 ttcctagaaa agaggctgaa gagagggcta gagaactttt aagattagtt gatttagaag
    19801 ataaattaaa ttcttatcca tttgaattgt caggaggaca gcaacagaga gttgctatag
    19861 ctaggtcatg tgcattgatg ccaaaagtcc tttgttttga tgaacctact tctgcacttg
    19921 atattgatac tattcaaaga gtagtcaata taatgaatag acttaaagac aaaggtatga
    19981 caatattaat aattacacat gatgttgtct tctcaaataa tgttgcagat aaaattataa
    20041 gtataaaaga tggtatagta gaaaatgttc aaataaaaga aaaaattgtt tagagtttta
    20101 taaatataaa ctagggtaga attttataat ataaataaag ggcaagtaaa atcaaatata
    20161 ctatgccctt ttgttatctc aaaatttaat cttatatttt atgtttaagt tacattataa
    20221 tttataataa aaataaaagg actatttaaa tatataatgt gaataaatgt ttaataattt
    20281 gaaatatgat aattttttat gttgaaagct tataatgatt atgatataaa ttggttatag
    20341 tatacaattt atgggtataa caataaaaaa ttagaaagga atttttataa aatgattaaa
    20401 ttatatggat atacaaaatg ctctactgtt aaaaaagcta aaaattggct taaggaaaat
    20461 aatttagaat ttgaagatat agatatggtt caaaaccctc ctagtaaaga ggagttaaaa
    20521 tcaatttata agactagtgg atatgatata aagaaatttt ttaatacaag tggtatgaaa
    20581 tatagagaat tgggtcttaa agatattgta aaaactgaat ctgatgataa acttttagaa
    20641 atattggtaa gtgatggtat gcttattaaa agaccattac ttttggatgg taaaaatgta
    20701 ttattgggct ttaaagaaga tgtatggaaa agtactttgc taaaagaaga ttaggaagag
    20761 ataggtgtgt tgattaaaat tattaattaa tacacctata aatttatgta ttaaatctaa
    20821 tctttataaa tattaattag caaattcaaa tcaaaaaaat tcatatcaac aaatatatta
    20881 cttcttatta caaattatat catatgtttt atattaaaat attataatat tacaacgaat
    20941 tgttacaaaa ttacttatta ttaaccatga acatttgttt tgtgctatca tatttatata
    21001 agggaattgt cataaaaaat atcttgttca tacagtttta tatagaaaaa agaa
//
LOCUS       DAEPOG010000059        20662 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEPOG010000059 DAEPOG010000000
VERSION     DAEPOG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.059"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            263..1048
                     /locus_tag="KR432_001795"
     CDS             263..1048
                     /locus_tag="KR432_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0640334.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(1419..2597)
                     /locus_tag="KR432_001796"
     CDS             complement(1419..2597)
                     /locus_tag="KR432_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBH0640335.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(2700..3464)
                     /locus_tag="KR432_001797"
     CDS             complement(2700..3464)
                     /locus_tag="KR432_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640336.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            complement(3653..4477)
                     /gene="pdaA"
                     /locus_tag="KR432_001798"
     CDS             complement(3653..4477)
                     /gene="pdaA"
                     /locus_tag="KR432_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0640337.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            4698..7817
                     /locus_tag="KR432_001799"
     CDS             4698..7817
                     /locus_tag="KR432_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0640338.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            7835..8434
                     /locus_tag="KR432_001800"
     CDS             7835..8434
                     /locus_tag="KR432_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBH0640339.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(8691..10820)
                     /locus_tag="KR432_001801"
     CDS             complement(8691..10820)
                     /locus_tag="KR432_001801"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBH0640340.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            11086..11334
                     /locus_tag="KR432_001802"
     CDS             11086..11334
                     /locus_tag="KR432_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640341.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            11500..12891
                     /locus_tag="KR432_001803"
     CDS             11500..12891
                     /locus_tag="KR432_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBH0640342.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            13006..14475
                     /locus_tag="KR432_001804"
     CDS             13006..14475
                     /locus_tag="KR432_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0640343.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            14540..15133
                     /locus_tag="KR432_001805"
     CDS             14540..15133
                     /locus_tag="KR432_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBH0640344.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            15315..16661
                     /locus_tag="KR432_001806"
     CDS             15315..16661
                     /locus_tag="KR432_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0640345.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            16843..17214
                     /locus_tag="KR432_001807"
     CDS             16843..17214
                     /locus_tag="KR432_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH0640346.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            17207..17896
                     /locus_tag="KR432_001808"
     CDS             17207..17896
                     /locus_tag="KR432_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBH0640347.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            18416..19282
                     /locus_tag="KR432_001809"
     CDS             18416..19282
                     /locus_tag="KR432_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0640348.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            19524..20333
                     /locus_tag="KR432_001810"
     CDS             19524..20333
                     /locus_tag="KR432_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640349.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            20476..>20662
                     /locus_tag="KR432_001811"
     CDS             20476..>20662
                     /locus_tag="KR432_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0640350.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNL"
BASE COUNT         8246 a         2188 c         3392 g         6836 t
ORIGIN      
        1 cttatgataa tttagaggag gaatatatat gaatatatca agaaaagcta tgaaaataat
       61 agaattagca caaaaaattg caaataagag aggtatttca gtagaagaag cttggagtga
      121 agcagtaaca gaatataaaa ataaatacga acatattgca tagatattta aaaatttaga
      181 atatttggta gaatataatg attttaaatg atactataaa ttataaggta tatcattatt
      241 aacatagaag gaggatatct gcttgattgt aaaaatagat aatactttag aaaaagaatt
      301 ttggcaatat gtatcacatg aagaaagttt aaatttattc ataattggat acgttgagaa
      361 ttatggattt agctctcaat atcaagatat atggtctcag gtagaagatg gaaatataac
      421 ctctataata ttaaaaaata agtcaacact tataatttat agttttaaaa ataattttga
      481 tataggagaa atgaagaatc atataaaaga tttagatgta gaaagcataa gtggaaaaaa
      541 atgtgtaata gatagactaa taagtaaata caaagatttt tatgaaaaac tagataataa
      601 attttgtgta ttaaaagaaa taaaagaaat agatttttcc aacatgaagg agtataaaat
      661 agaaaatgct caggaaaaag atattgatga aataggaaaa cttttaaata ggtcagacta
      721 taaagtaagt aaaaattaca tagaagaaag gaaagaacat ttaaaagagg gaaatgtaag
      781 agcatatttt attagaaatg atgatactat gattagcact gtgtcaactg gtatggaaac
      841 tagttttttg gcaatggtgg tctctgtaag tacagataaa agatataggg gaaaaggact
      901 tgcaagttat atggtttaca atttgagtaa agaattattg ttggaaggaa aagttccatg
      961 tcttttttat aataatgatg tagcaggtaa aatatatcat aatataggat acaaagagat
     1021 aaatgaatgg acaattcttt ttaaatagct ataaaaataa taaattttcg tgtaattctt
     1081 aacgtgtatt gtataatcgc tcaaatactt gaaaggtgtc tagtagatgg gtttgttagc
     1141 atttatggtc tccttatcta agtaaagttc cctttggaaa tcggtgaaat actcttttac
     1201 atttcatgag tcctataaaa tctatgacat aagaaagacc cagttacatt gagtaattgg
     1261 gtcttattat catatatatt gtgtgcttac atgatatagt tcatttctat aaacgtagta
     1321 tttcgtaagt gggaaccaat tttttaacaa gtgattattt gaacaaagca ataaggatat
     1381 gctagcagat ggattttgta taatttaaaa atcaatattt agactctatc atgtattttc
     1441 atcatcataa tccaatttga aattgtatct aaaaccagtt tgctatttcc acagttacag
     1501 tggttactag cttgttctgc ttctgtcata agcctaaatg taagggatcg gacattgttg
     1561 agtaaatcaa tctctttacc aacagttcta taatcaataa aatgatcttg tgttgcacct
     1621 aaaatcaaaa tgtcttggtt tatatttttt gcaatagaat ctatttgata atacttgatt
     1681 ttctgaaaat attcataggg tgtagacgct tcatatgcgt acattccatg ttttaaaccc
     1741 cataccacca tactatcgcc acttttcatt ttttgcttta ttataaaatt gactagccat
     1801 tttgttttga gttttagcag tagtctaagt agattttgca tttttattgg ttgtgaacca
     1861 actattatgt ctaagaaatt ggggaataca gaccaagcta ccacgcgttc aattcgtttt
     1921 tcgaaggctg ctgctcgtgg agcaagaaaa cctcccaagg aagcacctac aatagttata
     1981 ttctctaaat gaaaataatc taaaattgtc ttaacgggac gttcccattg ataagtaaag
     2041 tgttttccct gcaaacgcat cactccacct tgaccaggtc cctcaaaaag ataaatatca
     2101 aatccctgtg acgcaagata aagcattggg aaaaagaatt cttcaaagta agaatcattt
     2161 ccaccgtgga gcaatataac atcttttacc tcgccttgcg cttttgcata cattacaggt
     2221 agggtgatat tttcatatgg tacatgaaaa atttgtacag ttccatttga gaaatattca
     2281 gagtagtagt tgtagaatag agtagttgct tttttgtaga ctgatatttt atcagggtca
     2341 ccatcataca taaaaaattc actcatacga tagtaagcaa ttgcattttc tgtgcgtcca
     2401 tcttgttcag cttcttgtgc cagatacata agttctgttt tccaactttc gctatcgaca
     2461 attttagagg aaactttttg tatatcttcc aagcgccccc catcccacat aataatcctg
     2521 tttaactgaa aattaaaatt ggcatttgta ttaaggtaat atagaccttt attaaaagaa
     2581 attttatttg ttttcaatgt catcactcct aaaaagataa tgcatagcaa taaagagtaa
     2641 tctttaatgc tatgcattta ttgtaacaaa tattagaagt actgactttc aaatagacgc
     2701 tattggcgga taaaatatat tttacatttt cctataagct cacttgcagt aatgccaaga
     2761 tattttttgc tggaagctga gaaatgagac ggtgaactaa accctgcgtc catagcagct
     2821 gaggtaatat ctccactttt taacaataac tcataagcct tgcgaagttt tgataaagcc
     2881 aaatagctgt ttagtgatac tcccatttgt tctttgaaaa gatgagaaaa ccggcttttg
     2941 gacaatttta cttcggcaca tatctcaaaa aagatttcat tgtgaatgtg tgtggacttt
     3001 agaatatgat tcaatgccca ttgtattcta ctgtctgtaa tagacgaaat atgataattt
     3061 aaattcaata gacggaaagc ttgttttgaa aaatcaatat agctttggga caatcctttt
     3121 acctgacaag ctgtataaat attttgtatt cgttcaacta taagattatc tataacgaaa
     3181 aaagagtgat ttagtaattg gtgttgacta atattttggg cgagtatgct tgtatcatct
     3241 atcaagaaaa ccaacatttt attatcttta cttgacaccg tatgcgatac attggaatca
     3301 ataataattc ctttacattg taatgattcg cccttatcaa acgtcacaga taagttacca
     3361 gactgtgata taataatatg ttttgcaaga tgtttatgga tagttggttg tctatactct
     3421 gtatctaaaa atatatggtc aatcattaca caaatggtag tcatagctgt caactccttg
     3481 ttcatagctt agcatattat acagttttta acaagaaagc ttagtgatta caagtattta
     3541 aaaatcctat tttaggcaat gaaaaataaa aaactctatc gagaattaaa gtatttataa
     3601 ataactatat ttttactatg attcatgata gagtttttta aaatttatga tactattttt
     3661 tattatttaa ataatcaagt gttttaagtt tataaccttc tttttcccaa tgtgtaagaa
     3721 cctcatctaa tatctcagta tttgttgatg aatttggatg cagtaaaact attgcaccag
     3781 gatgagttct tgaataaatt ttgtttttgg caaattcatg agtaggttgt ttcttttcat
     3841 accaatctac ataagcaaaa ctccaaaata ttgatttata tcctaaatct tgtgtatatt
     3901 ttaaagattg ttcgctaaat ttacccattg gaggtctaaa atacttaggc atttcttttc
     3961 cagtaacatc tttgtatgct ttttctacac ttgtaatttc ttctttgaat ttttcaaaat
     4021 ctgtaatacc tgccattgat ggatggcttt ttgaatgatt acaaactaaa tgtccttctt
     4081 tttccatacg ctttattaac tctggattgc tttttatata actttcaact acgaaaaatt
     4141 gagctttagc attgtgtttt ttaagcgtat ctaataattt tggagtgttt ccactttcat
     4201 atccagcatc aaatgataga tatataactt tttcattggg atttcctaca taataagctc
     4261 cgtatttttt aaaaaaatca gcttctttta taggtgatgg ctgtttacca tcctccctag
     4321 ggttaaaata ccaatcatat tcatgtgtgt cgagtgttgg actagaaatt tgagtttttg
     4381 ttttatctaa tgatttaaaa tttatactag caattccaaa aagtattaga gcaaatgctc
     4441 ctatcataat gtattttttt aggctatctt ttttcacaat ttcacctctt atttaaaatt
     4501 taaagtatat ttcttataat atagtttgaa tatttatagt taaaaatatt cacatataca
     4561 aataataaat gaaagaagtt tactgtaaga tataaagttg atattaatta tcttaaataa
     4621 agtgtatata aatgataaaa tataaagaga aatgattgta aataagttag gctggtttaa
     4681 cttgaaggga tataaaaatg gataatataa taagtgtatt gaatgatgtt gtttttttag
     4741 atatagaagt tagtggatta gattgtttaa attcagaaat tttagaagtt ggagctgtca
     4801 aggttaaaga ctggaaaata tatacctatg aatcattaat aaaaaacaaa tttgaggtac
     4861 ctatagaagt attctcagta tgtaaaaatt tagataaaaa tgatttagaa atagctaatg
     4921 aaatagaact ggtggaagat agacttgtaa attttgtaga agatagtttc attatatgtc
     4981 atgatttaag tctgaaaaaa aagttttttg agtatcatat gccaaagtta aaaaacaaat
     5041 ttattgattt aattgaatta gcagttattt tagaaccata tcataaagat tatagcttag
     5101 aatacttaaa aaatacttta acaaattgta attctaaagt ggaaaataga gctttatcag
     5161 atgctattga tataataaat attgtaaatt gccttttagt aaaatttaat aactatgaaa
     5221 aaactacttt ggagccattg agttttaaaa taaattcata tcttaaaaaa tttaatcttc
     5281 caacatggga atggagcaaa tttttagaag aggcaaatta tgatttatca aataatataa
     5341 atataaaaaa agaatataat atatttgact caaaagaaga aaagaagaag gaaagagaaa
     5401 ccttaaaaat tttaaatgaa gaagaaaaaa attatgaaga attattaaaa tacaaaacta
     5461 tttgggagaa taaagaagga tttacatatg aatatagacc tggtcaatat gagcttacta
     5521 aaacaataag agagttgttt agaaatagcg aagatgaaga aaaaattgca tgtatagagg
     5581 caccaactgg tataggtaaa agtgtaggat atttattgcc tgcaatatta gaggctagaa
     5641 ttaataaaaa aagattatta atttctactg acacaaagga attgcagata caattaataa
     5701 ataaagatat tccaaatgtg ttagattctt tgggtctaaa tggaaaagta agttacggat
     5761 atataaaggg aaaaaataac tatatttgta tagatagact ggaagcatat atagatgatt
     5821 atgagtctca aaaccctact aaaggagaaa ttttatcttt aatatttttg aagagattag
     5881 ttgaaggtgg caagtatgga gatatagaag aaataaatta tttagtgttt gacaacttca
     5941 aggaaataag tacccactta agaaatgtaa gttgtgaccc aaatatgtgc agacctaaaa
     6001 aatgcaaaaa agattgttta tataaaaaca gaatagaaga attaaaagaa gaacatataa
     6061 cagtagtaaa tcattcacta ttagctaaat ggccgtataa ggatgaaaaa cctttagaaa
     6121 atataatagt agatgaagca cataatttga ctgaaaaagg atatgatttc ttttcaagca
     6181 ttataaattc aaagtcttta aggtatttat tacaagaaat ttatccttac gagtttattc
     6241 aaaatagtag ctttatatat aaaaaatata gtagaaatat gagaaaaata aaggcttttg
     6301 ataaatttta taatgtatta aaaattggaa gagaagataa acaaaaaatt gctagaagta
     6361 taaatctcat tattgaagag atagattcaa tattaaagtt tggtaattgt aatgaatata
     6421 ataatgtaag taattataat ttaagatggg aactgaattt gcaaatagat gaaattgtgg
     6481 gaaaactaaa aaaggatggt atagatactg aaataagtta tagagcctat agtgaaaaga
     6541 taaagttgag ttgtgagaaa ataataaaaa atttagtttc aataataata ataatttata
     6601 ggaatattga tgatgatagt atagataaag aagcagatat atataaattt ggaaaggcaa
     6661 agacaagaga tttagaggat ataaagatta tatttgaaat ctttttagag tatgatgaaa
     6721 aggatgatta tgcaaggata gttgaaattg ataagaatta taatgacttt gaatttcgag
     6781 tagtaccatt aaaaatagca gatttatttg aagaaaatat tttatctcaa ttagaaaaag
     6841 gaatattttt atcagcaact ttgagtttat ctgaaagtat gtcttatttt aaaaatacat
     6901 taggaataga cagagttaag aatgtggaaa agattataga accaattttt gattataaaa
     6961 atagagtctc tgtagttgga ttttctgata tttgtgagta cagaaattct gaatttccaa
     7021 atgagatgag taaaattata tctaatatat caaagattac agaaggtcat acattagctc
     7081 tttttaatag taaagataga caagaaaaaa cttatgaaat attgaaaaaa tacttacata
     7141 gttttaactt agaaatttat gctgataaaa aggggattag acaccttaac gatttaaata
     7201 gaaaatgtgt agtacttggc tcaaaaggat gttttgaagg cgtagacatt ccaggggatg
     7261 gtttggtttg tgtaacttta gacaagcttc cgaatctaaa tccaaaagac cctttgtatt
     7321 ttactataat gaagaagtat ggtatagact actatactat aaattatcct caaatgacca
     7381 taaaggtaaa gcaagctatg gggagaattt taagaagcaa atatgactat ggatgttttg
     7441 tgatttttga tgtaggaact aatataagtg ttttaaagag attagagaaa gatttacacg
     7501 attgtaaaat ttcaaaggtt aatagcaatg agttttatac ttatataagg cgacatttaa
     7561 ataaatctag aagtttaata ctaaaatctg ttatatttga tactataaaa gcgttgaatg
     7621 ttgatgctaa aatggataac aatgatgttg ataaagatat tataaaaaaa gatataaatg
     7681 aaaatataag acaaagagct gtaaaaggag aagtgtacca tattgatatt ataaaaaaag
     7741 atatgaaagt aaaatatttt gatagaaact atcttataaa tttagatata tttatgagag
     7801 aagaagataa aaattaagag tgggagtgag atatttggca aaacaaaagt tttatgctgt
     7861 gaaaaaaggt aaaaatatag gtgtatataa tacatgggat gagtgcaaaa aacaagtaaa
     7921 tggattctca ggagctgaat ataagagttt ttctactttt caagaagcta aagaatatat
     7981 tgatgggtca gaaaaattga gttttcaaga agataaggag tttatagaag catatgtaga
     8041 tggaagttat gaacattcag taaaaatgta tggttcgggt gttgtgattt taaaaaataa
     8101 tgaagttata aaaacttata gtgaaaaagg aaaagagaaa actttagtaa gtatgagaaa
     8161 tgtagctgga gagatagagg cttctaaaat tgcaatgcag tactgtattg acaataatgt
     8221 tcaaaacttg attttatact ttgactatga agggattgaa aagtggtgta ctggagtttg
     8281 gaaaacaaat aaagaaggaa ctatagcata taaaaatttc tatgacagta ttaagaataa
     8341 gcttaatgta aaatttacaa aggttaaagc tcattctgga aataagtata atgaggaagc
     8401 tgataaatta gctaaaaaag ctattggtgt ataataaaaa aatatttata cataattaca
     8461 tggtataaga gtatatctaa attcctactt tataattaaa caacttgtaa ttaaacaact
     8521 tgtaattaaa caacttataa ttaaataaat tttgattaaa aaagcagatt gtattatgaa
     8581 aaaataagta tctaatttat aaacaattaa taatagtatt aattagatac aacttattta
     8641 atataataaa atctgctttt tatataacta taaaatcttc ctacattcta ttagaattgc
     8701 ccataaatac cttcaagttc ttcttttaat acttcagggt catttatacc aatagacatg
     8761 gttctagcca gtatatttgc ttggtcataa gttgatgtcc atgagaatac accacctaga
     8821 caatttctaa gaacatattg agtttttaag aatattgata aagcatcatc atatgacatt
     8881 gcaaaatctc catccttaac taaatacgga acttgagcag tattatcaaa tctatattca
     8941 tatccatttt tatttatata atcccttcta agctcatcat aagttcttgt tatagtggca
     9001 cctaatcttc cataaaatgg tataccgaga aggatttttt cagaaggcat tccagcattc
     9061 tcaagatttc tcaccattgc atcaacacta tatcctggca aagataagtc tgaatcaaaa
     9121 agatttgctt gatgttttct accatttggg cctgtttcac ctgctgtaaa atcataactc
     9181 ataagattaa aataatctat tataggagct attttatcta tttcagcact tgaatttata
     9241 tatcctctat ctcctgttcc agctacacta agccatttat catctcctat aacatctctt
     9301 atggcagtta gtaagagtgt aaagttttct ctatcttgag gtcttgatgt tattccagat
     9361 gcactacttc caggatattc ccagtctata tctattccat ctaaagcata ttcatttatc
     9421 atctgattga cctgtcttgc aaaattatat ctagatgtag gtgttaatgc tgcatcagag
     9481 aatccttcag ctccccaacc tccaatagct acaattactt ttaagctagg tttttcacct
     9541 ttaagagcaa gtaattgatt taaaaatcta ggataaggaa taaacaaatt tccagactgg
     9601 tctatctcag caaaagcaaa tataactgca tctaatagtt gagcatctac attagttgga
     9661 tttccaagaa catattctgc tacaattgga tttatatctg gacagtttgc attttcaact
     9721 attggattgt atgggtctgt tatttgtggt ctaactaatt tttttgaatc ttttaaagtt
     9781 ttcatagcta tttttttagt atttttttcg ctagtataat cgtagttttt ataattatgt
     9841 ttatagaaat cttcataact actatcttct ttgttatagc tagaatttat tttgtcgcaa
     9901 gattcatctt tatcatagtt aaaattcatt tcatcacaag acttatcttt aaacttatct
     9961 tgtttggagt tgtgaacttt gttagtattt ttagtatgtt ttttctcatg gttgcaatca
    10021 tctttaatat cagtcttgtg ttcttgttta cagtcaagag ctttgttcag gtagtcatga
    10081 tgttcatgtt cacagttaca ttcattttca atgtttttag tatgttcttt tttccagcta
    10141 caagtattat caattttctt gctcacttct tcatcattat aattatctgg tacaaaacat
    10201 aagtaccagt ccatacatga atatttctta ttacaactgt taactctatt ttttaattcc
    10261 ttatagtttt taggataagg taaaatcact ggcattccag gaaaccagtt tgcaggagta
    10321 actatattat ctctatcact agtctgaagt gcatctacta ttctaagtat ttctggaata
    10381 tttcttcctg tagttagtgg ataataaaga atcgttctta gaatttgatt attatctata
    10441 ataaatacag agcgaacagt tgatgtatca ctcattggtt ttgatatcat gccgtataac
    10501 ttggcaattc tcatatctct atcttctata ataggaaatg gaatttctac acctgtaagt
    10561 aaagaaatat tatacatcca agctaaatgt gaactgttac tatcaacact tagaccaatt
    10621 agttctgtat ttcttttttt aaattcgtca taatatttag caaaacataa aaattctgta
    10681 gtacaaactg gtgtaaaatc accaggatgt gaaaataaaa caatccaatt acccttatag
    10741 tcagagagtc taataggacc atttgttgta ttggctttaa aatcaggagc ctttgaccct
    10801 aaacttggca aatttggcat gtaaatcacc cctcaataaa ttatcattta ttttattcta
    10861 ttaattaata catattagtg ttactatttt attaaaagtt aagtaatcct tatgtcaaaa
    10921 aaagatattt attaaaaaga tttaatattt tcctaatata aatgacaata gtaattgcta
    10981 tttttttact aatttggtat agtaatggat aaggggtagt gtcaatttta gtgattatac
    11041 gaggtgattg gattgaaaaa aattctaaga attttagtta tctccatgat gatatgtttt
    11101 gcaatgactt ttgcattagt gttaataaat tcagttatgg gagtacatat aggattggat
    11161 tattactttt tgatgggaag tgtgcttaca gtttgtttaa caattggaat tgttttatgc
    11221 aatgctagaa aaatatttga tagcaaagct atacacaaga caagaacgaa aactgtcagt
    11281 actaataaaa aacaaaagag tcattctagg gatataaaaa gaaagatatc ataatttatg
    11341 taaaggaaat atttatgtat aaaataatga ttgataggta aaataaaaac acgatttaac
    11401 ttgtgttttt attttttttc aagaattatg atataataac caataagaaa tttagtttac
    11461 cataaaatat aaagattatt aaatggagag ggaactataa tgaattttag agatttattt
    11521 gagaaggctg tagattttat tgatgaaaaa aggaaatcta tagttgcgat ttcattatca
    11581 acattaggtg taattgcttt gataatagta tttttcttaa gtagtaacga gtttagtgta
    11641 ggaaatgaag ctaatgagtt gttaaagatt atagagaaaa gacagtattc tatagctgtt
    11701 gattactaca caagtataga aaaaaagttt tctgattcaa aaatggaaag gtttaataag
    11761 tctgtttcta aaaaaataaa taaattgcta ttaaatagtg gtgataaata tttagatgga
    11821 gacataagtc aagaaagttt tattggatta ataaatactg tgaaagaatt aaacaaaata
    11881 agtatagatg tggatgactt attagctcaa gctgatagag ttagagaaat gtataaagaa
    11941 gaaaatacta cttatgatat tgccattaac tacataagta ctgtttcaat attaaatggt
    12001 atgggtagta acctagatgt atacaaacag aatatagaaa caatcaaaga gtctagagat
    12061 gtttatgact cagcagtaaa agaccaaaaa gcatataagt attatgaagc tattgaaaag
    12121 tataataaag tattaaaaga agatgaaaag tattacagta tggctcaaaa tgggaaagaa
    12181 caatgtattg aagaaatgta tgattactat ataagtagag ctgaagaggc aaatacttct
    12241 ggagattatg aaagagcatt acaatatatt gagtatttaa aagaagatta ttcagatgat
    12301 gaaaaggtac agagtttaga aaaaaaatat aaaaagaatt tatcattgta taccttaaca
    12361 tctgatgaca ttataaatgt aatagctaaa aaaagtggca aagacaaggc aaatctatct
    12421 ataaattcac ttccacaaat gataaaaaat aataaatact attatgctga agtatatgag
    12481 tatgataaat taataaatga agttttggta agtgcaaaaa ctaaagattt atattcgtat
    12541 aaagatggta aaaaagatta caaagtagat tatggtgatg gatactttag aatattagaa
    12601 gatggaagtt atcaatttgg tattacaaaa gataaagcta agtttgtgtt aactaatact
    12661 cttgatgaaa aagaaaataa gtataaaaag attgagatat tagatataga gaaagcagat
    12721 agatatgtaa aaagtaaaaa gagtttagaa gaattgtttg gaaaatataa aaatatttat
    12781 tattatgctg tagtaaataa aggcttattt agaggcaaag aagtttatgc tattaatatt
    12841 tacaatgaaa aaatatatgc catatcagaa aatggactaa atgaatatta attaataata
    12901 ttaaattaat atataattaa aaagtttttg gggttttaag gcgcattatg tagtaatata
    12961 ataataggga tatataaata attttattgt aaggagaagt atgggatgag taatgtgaac
    13021 aaaaagctag ttatagctat tggagctgta gcattagtag caataatttt tataggcata
    13081 atgtgcatgc aatttaaagg ggagaaaata gcaaaaaaca catatgttaa tggagtagat
    13141 attggaaaat taactaaatc gcaagcaaaa caggaattag ctaaaaaata caaattagag
    13201 aatgttgagt ttaattacaa tgataaaagt tggaaagtaa agagcaaaga tttaaattta
    13261 tctactgatc tggataaaac agtagaaaac gcatataatt taaatagaaa gagtagtttc
    13321 tttggcaatc tatcaaaaac tatttcaaca aattttggca aaaaaagcaa cttagtagtt
    13381 gtgataaatt atgataaaaa taaattaaaa gcagaaatgg aaaaaatagc taaagaaata
    13441 gatgtagatg taaaagatgc tacacttgat ataagtggtg aaaaagtcaa ggttatacca
    13501 gattctgatg gtcttaaaat ggatatttct aagagtatgg agaattttga taatcaaact
    13561 aaaaaaggta actataaaaa tgagttagta gtaaaagcta ctccagcaaa agtaaaaaaa
    13621 gagcaactta ctaatataga tacaaattta ggtacgtatt caacaatatt taaaacttct
    13681 caaataaata gaagtataaa cataaaatta gcaacagata atataagtaa tgtattactt
    13741 atgccaggag aaactttctc atttaataaa catacaggga aaagaagtaa ggaaaatgga
    13801 tataaaagtg cacctgtaat aatggaaggt gagatggaag aagattatgg tggaggagtt
    13861 tgtcaagttt catctacgtt atacaattct gtgttatacg caggtttaga aattgttaat
    13921 gtaaaaaatc atactatacc atcaagttat gttccaaaag gaagagatgc aactgttgca
    13981 gatagtggaa tagatttctt atttaaaaat aatctaaagc atccagttta cataaaaaat
    14041 tacgtttcag gaaatcaaat tgtttgcaat atatatggaa gtgcagaaga taaacaaaat
    14101 ataacgatat caacaaaatt agatggcgtt tcacaaacta caatgaaaag agttaatgac
    14161 cctacaatgc ctaaaggtaa agaaaaagta gacaaaagtg gaagaaatgc atattctgta
    14221 tcaacatata gaacatttaa tgatgcaaat ggtaaaaaaa ttaaaactga aaaaatagct
    14281 aattcatatt atcctaagaa ggaaggtatt atattagttg ggactatgga accaaaacca
    14341 gaggaaaaac caaatacaga cgaaaataag aataatcaaa atacaaacaa tcaaaatcca
    14401 aataatcaac aaaaaccaga aacaccacca actgataata agcctaatga gacacaacca
    14461 caaccgcaag cttaaagttc tcaagaatag gatttatgta gagtaataaa aaatataaat
    14521 ctaaatagga gggattactt tgaatttaaa taatataacg aattacgttc aaagtatttg
    14581 cgagaatatt tctaatgtat tggatgtaga tgttacttta gttacaaaag atttaactag
    14641 gatagcaggt acaggaatat tcagaggtaa aataggagag acaatatcag agaagtcagt
    14701 gtactctctt gttttaaaag atggaaaatc ctatgttata aataaggaaa tagaagagaa
    14761 ctgtaataaa tgtgcaaata gagaaaattg taaagaatta gctgatatat gtactcctgt
    14821 taaaatggga gaacatatac ttggaatctt gggtatagct gcgtttaatg aagagcaaaa
    14881 agaaaagata ctttcaaaag ataaggagtt aactgctttt gtaaggagta tgtctgattt
    14941 aatatctttt aaactagatg aactttataa acaagaagtt aagactatag atgaccttga
    15001 gagggaagct atagaaaatg ccataaagaa atatggaagt aatacagaag gtatgaaaaa
    15061 agtagctgag gcattaaata taggtatagc tacactctat agaaaagtaa aaaaattcaa
    15121 cattaaaagt taatagtatt gaaaaagagg taaccttttt atgaaaagaa gaggattacc
    15181 ttttttatta tgagaatggt ggaaaaatat gtatagtttt gacttgacat attaaatatg
    15241 ttatcttaaa tatatatttt agagtggagg tgagtattat tttagttaaa gatataaaac
    15301 aagacataga aagcatgcct agaaaaattt tagatatatc tttgatagaa gataataaaa
    15361 gtgatttgac tttttttaat atagaagaag aagacggtga tgaaacatta aaagcattaa
    15421 aacagagtta tgtgactaca aattataaag aagaaactct attaaacttg caaggagctt
    15481 taaattcaaa gtcagacaca acgtctaagg agttaaaatt agaatcatat aaaagatata
    15541 acaatgcatt ggatttagca tataaaaatt atataactag tgccattaat atggttaata
    15601 aagctctgga gataaatcct aaagatattg atatactaaa tcttagaggt cttttaaagt
    15661 tattaaaatg tgattttgct aaatcatttg agagttttta tactgcttta tgttatgaga
    15721 ataatcatat atctagaaaa tatgtaaatc taatttcatc tgaagatttt aaaacttttt
    15781 taggaagata caatcattct ataagattta taaatgaaga attaaattat gagtcaatac
    15841 atattttaga gaacataatt gaagaagagc ctgagctaat agagccatat gtaatcttat
    15901 ctttgttata tgataaacta ggtaatgtaa aaaagagaga aggatattta gacaaactta
    15961 aagagctaga taaagataat cctgtatttg aaaaaaatga agaagaaaaa gaagatacaa
    16021 gtaaaaagga agaaataaga aaagtaaaaa gaaagaaaag tattttacca tatattatag
    16081 ttggctgtgt tttaataggt atggggatat atttgattca aagcaaaaag agaatagaaa
    16141 acttgaataa ccaaataagt agtaaagagg aaaagttaag tgaaactgat aaaaagttag
    16201 gagaaacgag taaaaaatta gaaaaaacga ataaggaatt aaatgaggtt aaaaataaag
    16261 aaccagaaag agagataact gtagctgatg aggaagattt atattatcaa gctctaaatt
    16321 taaagaaaaa caaagaatat gaaaaagcca tagataattt taaaagtgta gtttcttctg
    16381 gtaaaactaa aaaatatatt tctgagtcaa tatatcaatt agcaataaca aataaattat
    16441 taggaaataa ggatgaagct ataaaatatt acaaaaaata tataaatact tatactaaaa
    16501 atgaccagta ttatgatgat tcatattacg agttgggtat gctttattat gataatggcg
    16561 atttaaaaaa tgcacaacag actttttata gtttgagaag tgaagttcca gacagtatgt
    16621 ataacaattc aaaaataaaa gaaatcttaa gtgaaaaata ggtttatata gttaagtttt
    16681 ttgaaacaac caagtatgta ttttattaaa ttaaaataaa aataaattta aaaagagatt
    16741 gtgtcaaatt ataactttct attttaaaga aagatatttg cttgttgata aaactacaaa
    16801 tatatgattt gaagcaatct ctttaatttt tggaggtgga aaatgaaagt tttcaatcaa
    16861 ttagtaataa ttttatctat ctgggtaata ggagagtata taagttcctt tatacaagga
    16921 attatactta ttcctgggag tatagttggt atgctattat tatttctact actacaattt
    16981 aaagttttag acttgagtag tatagaaaat gtaagtggat ttttcctcga taatatggct
    17041 atatttttta ttccagcagg tgtatcactt ataaagtcat tagacctaat aagggaaaat
    17101 gtattagtgc ttttattagt catatgttta agtacactaa tagtaatgta tactactgga
    17161 attattgtgg agaaaatgat aaaaaagaag gaagaaggac aaaaaaatgt ataatgtatt
    17221 acagacacca gtatttggta taatagtaac aatagttttt ttcaatttag gaagatacat
    17281 tcaacgaaaa acaagtaatc ctatatgtaa ccctttatta attgcaatcg taggaataat
    17341 attgttttta agcatatcta aaatccctta tgagaattac aagataggag ctgatagtct
    17401 aaatttcttt ttaggtcctg tcacagtagt acttgcagtt ccactttata aacaatttga
    17461 attatttaaa aagcatatgt ttgagattat tgtaggaata ggttgtggta tagtgatttc
    17521 ttttgtatct gtattgataa ttggcaaaat agctaattca gatgtaagta taataaattc
    17581 actaattcca aaatctataa caacaccaat gggaatatct ttagcaaatt ctttaaatgg
    17641 tatagaatca ataacagttg tagccataat ttttacaggt atttttggtg gtatggttgc
    17701 ttctacagta ttcaaactgg ggaatataaa tcatccagtt gctaaaggaa ttgctcttgg
    17761 aacatctgca catgctttag gaactacaaa agcttttgaa cttggagaaa tagaaggagc
    17821 tatgagtggt gtttctattg gagtttctgg tacaattaca gtaatactaa ttccaattat
    17881 aatgaatttt atataaaaaa taaaattaag acgagtacct tgaatactta aaacaaaata
    17941 aaataaaaaa aatttaaaat aaaaactgaa cgaaaaaaat gcaaataatg aaataaaaaa
    18001 atcatgtact gaaattataa aaagaaaaat ttcatttagg aattaattat aaagagataa
    18061 ttactaaact tgaatttgct aaaataaaat aaattactgc gaaaatagat atttagaaaa
    18121 aaatactata aatttactat attgcaagtt aaataataag cagtatcata ttatgctaga
    18181 aaaagtttca tttaaggcat aaatgagatg atttgaacgt ttttaaagat tagaatataa
    18241 aagattgcac tagacatata tgaaaaagat aatataatag atgatgtaat agatgacagt
    18301 tcaagaaatt ataaaaaaaa tttatttgaa atattatcta ttacatcagg aataatatat
    18361 gtaagagtaa gcttttaaaa ttctaaagat ttagaaagat tgaagggaga aagaaatgag
    18421 tgaagttaag aataccctac aaaaacatcc agttttaaga tattcaacaa tgctatttgg
    18481 gatgttgatg acatctatag gaataaatgg gttcttaaga ccagcacatc tgcttagtgg
    18541 aggtgctaca ggtatagcaa catctataaa ctatttgaca aatataaatg taggtttatt
    18601 aacattttta atcaatattc caatatttat cttagggttc atataccttg aaaaagagtt
    18661 ttgtatttca agtttggtaa atatgattgt attttcatta ttattaggag caactcagga
    18721 aataagtaat attataccta tacatgatat attgcttcaa agtgtatatg gtggtatatt
    18781 atctggttta ggagtcggag ttgtatttag aactaggtct tctcaaggtg gtacagatat
    18841 aatagctgca atactaaaaa ttaagaagaa tatagaaatg aaagacactg ctttagcaat
    18901 aaatggcttg atagtactta caggaagttt tttatttggg ttggatttag cattatacac
    18961 tcttataggt ctatttctaa atgcgtattc aatgagtttt attaaagatg caatgaatta
    19021 ccaaaaatct gttatggtaa tgtcaaatga agtcgattta atagcagaag atataatgaa
    19081 gagtttagtt agaggtgtaa cttttttaga tgcagaaggt gcatacactc atcaaaaaaa
    19141 gaagattata tatacaatag tttcatcaaa tgagatacca aagataaaag atatagcttt
    19201 gaagtatgat aaaaaggcct ttatatctgt aaatgatgtt acagaagtaa aaggaagagg
    19261 gtttaaagca aaagatttat agaaaatttt acaagctggg agtttataat gtaaatttgt
    19321 atattatatc tctcagctta tttaaattta gtatatgaaa ataaaggttt tatcaatgat
    19381 ttgtgtatca ctttaaaaga tatcatttta aaattaaaat tgaaaaaatt actcaatatg
    19441 tagaaaacat aaccactatt aggatataat aaatttaata gatttagtaa aatatctagg
    19501 aataacgttg gaggaatagc attatgaatt tatctaaaga atatgttaat ttgtatgata
    19561 attatttgaa atatataaat gaagtaaaca aagagattag aagtataaag aacatgaaga
    19621 aacgtgtttt aaaaaagggc gattttgctc cagaaaatga agtattagaa ttggaaggaa
    19681 aaacaatacg cgatatagat gatgtcgata caaaaaaacc aaaaaataaa atatataaat
    19741 tttcaaatgg ggatgtgtat gtaggaaaat ttttagaagg aaagatggaa gggcaaggat
    19801 catatacttt ttttgttgat gaaggaacgg ctatggagta cataggagag tttaaagaag
    19861 acaaaaaaaa cggaaaagga aactttgtat tttcaaatgg aaatgaatat ataggccatt
    19921 ttgaggaaga tatgatgaac ggtattggaa atatgttata taatagcaaa gatgaatata
    19981 ttggaacttg gaaaaatgga aaaaaagatg gaaaaggtat ttataagtgg agtgatggat
    20041 gtatatatgc aggtgaattt aaaggtggaa agatggaagg gtatggtatt tgctataata
    20101 gtaaagggga agtactttat gaaggagaat ggaagaataa tttgattcat ggtaaaggaa
    20161 cttatatatg ggaaaaaggt aaaaagtata taggtgaatt tatgcatggt aaaaaacatg
    20221 gacaaggaac tttttatcta aataatgaat tggtttatga agggacttgg aaatttgata
    20281 agccatccat cttcgacaga acattagatg aaatattttc attaagacta tagctttatc
    20341 tattaataga taaagctttt ttctaatact tactgtaaat aagtatgaat gatattgatt
    20401 agtatacact atcaatatta actcaataaa tgtgatatta aaaatttttg attgatttta
    20461 agaaggaggg atatattgaa gagattaatt gctttagtgt tagtaatact tattgtttta
    20521 ttttatagcc ctgaattgat agaagaagga tatgatttaa aagaaaaatt ttttcaaagt
    20581 gaaatatgga atgaattttt agacaaaata aatataaata ataattctaa gaaagataaa
    20641 aaagaacaaa atcaaaattt ac
//
LOCUS       DAEPOG010000060        20476 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEPOG010000060 DAEPOG010000000
VERSION     DAEPOG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.060"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..366
                     /locus_tag="KR432_001812"
     CDS             <1..366
                     /locus_tag="KR432_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640351.1"
                     /translation="KIINSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKD
                     TNEINNIRLFEVAYEHMIDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILV
                     QGKYIPQEALNRFDLEEYN"
     gene            366..1985
                     /locus_tag="KR432_001813"
     CDS             366..1985
                     /locus_tag="KR432_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640352.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            2003..2731
                     /locus_tag="KR432_001814"
     CDS             2003..2731
                     /locus_tag="KR432_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640353.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            2718..7073
                     /locus_tag="KR432_001815"
     CDS             2718..7073
                     /locus_tag="KR432_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640354.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            7175..7384
                     /locus_tag="KR432_001816"
     CDS             7175..7384
                     /locus_tag="KR432_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640355.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            7780..7920
                     /locus_tag="KR432_001817"
     CDS             7780..7920
                     /locus_tag="KR432_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640356.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            8058..9020
                     /locus_tag="KR432_001818"
     CDS             8058..9020
                     /locus_tag="KR432_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0640357.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            9098..9727
                     /locus_tag="KR432_001819"
     CDS             9098..9727
                     /locus_tag="KR432_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBH0640358.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            10558..10974
                     /locus_tag="KR432_001820"
     CDS             10558..10974
                     /locus_tag="KR432_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640359.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            10975..11187
                     /locus_tag="KR432_001821"
     CDS             10975..11187
                     /locus_tag="KR432_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640360.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            11822..12865
                     /locus_tag="KR432_001822"
     CDS             11822..12865
                     /locus_tag="KR432_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0640361.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            13126..14000
                     /locus_tag="KR432_001823"
                     /pseudo
     CDS             13126..14000
                     /locus_tag="KR432_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14019..15011
                     /locus_tag="KR432_001824"
     CDS             14019..15011
                     /locus_tag="KR432_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640362.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            15013..16707
                     /locus_tag="KR432_001825"
     CDS             15013..16707
                     /locus_tag="KR432_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0640363.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            17258..18136
                     /locus_tag="KR432_001826"
     CDS             17258..18136
                     /locus_tag="KR432_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBH0640364.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            18172..20067
                     /locus_tag="KR432_001827"
     CDS             18172..20067
                     /locus_tag="KR432_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0640365.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            20335..>20476
                     /locus_tag="KR432_001828"
     CDS             20335..>20476
                     /locus_tag="KR432_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0640366.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYL"
BASE COUNT         8653 a         2088 c         3379 g         6356 t
ORIGIN      
        1 aaaataatca acagtattaa aggattaaag gaagatataa caattgaata tcttacaaat
       61 aaagaaaatg aatttttata ttggggagat atagataaaa ctggattatc aatatacact
      121 tccctaaaag ataccaatga aattaataat ataagattat ttgaagttgc ttatgagcat
      181 atgatagata aaacaaagga aaataccttg aggaatatcc ccaaaaatca aaaagataat
      241 tttgaatgtg gattagaaaa tattaataac ctaaacttaa aagaaagtat taaaaatatt
      301 ttagtacaag gaaaatacat acctcaagaa gctctaaata gattcgactt ggaggaatat
      361 aactaatgga aatgattaat attttagaag gctacaataa gagagtaaac tgtataggat
      421 cttattatct tattgcaact cttgctaaca atagaaataa atttaaagaa tttgacaaca
      481 ttcaatttta caatttatta attcaagttt tgtgttatat atttgataga tctcttagaa
      541 gaaagaactg tttaagagat gatattaagg attttattga agaaataaat cgtatggatt
      601 ataaaataat gctttcagag gatgacttaa aggatttagc aaactatata attaatggat
      661 taaccaatag tggtaaagta tatctattta cctactattc tttagaacaa gaaaaacata
      721 tagatgaaag tattaaaatt atagaagata aaaatgtaaa aataaataat caggaaagac
      781 tatcctactc cttaacaaca gagggttata gattgttgtt atcaacaaaa gaatatgatg
      841 aactattcca aattcaaata tcacaaatga ttgcaaagtt aaggatagaa aagggtgatt
      901 atcaaggcgc aaaggaagat atttacgata taataaatac cttagagatt catcaacaga
      961 aaattgataa ttatataaaa ataataaaag taaatatact atatataaaa gaagaaaaat
     1021 attcaacagt aatagataca actgtagaaa cacttttaga agaaatggac aaatatgaag
     1081 aattaaaaaa agaagtaaaa ctattaatca aagataaaga ggactacttg gaaaaggatg
     1141 aggatataga caaagaaaag ataaaaaata taagaactca actattacaa atgaaagaat
     1201 taatagatgg aatagtttta gcaaagtcta cagccacaaa tttgttatct agagttcaag
     1261 attttagaga agaatttaaa gaaatactta aaaaactatt aacaactcca gtaattaaaa
     1321 gattcaattt caaggaagaa atcttagata gacttgaaaa agacagtgat tttttattga
     1381 taataagaga attatatata tctttattta aagtagagtt gccaaatcga tttaatattg
     1441 gagtggcata tcaagaacag aaactattaa atactagaga aattgaaaat gaaatagtta
     1501 ataaagacga gttagaaatt attgaaaata aagagaagga ggaacgtata aattattgtg
     1561 aaaattacta ttttgatttt tttaaatact taattaaata tggacttgaa aaaggacaat
     1621 ttacattaga agaatttcta aaaaattatc tgaaatatga caaagttatt tacttagaaa
     1681 taactaaaga ctccaaattg atgagagata taataattta cttcgctaat caaagggaaa
     1741 gtatcaatat caaaaagata atcagaatgt tagaaaatag tgccttctca ccaaatacag
     1801 attttcaaat tgaaagatta atagaacgtt tattagtaga agattttgaa ctattaaata
     1861 gcgttagcca tattaaaagt aataaaatat ttgaagatga aatgaaaata aatactgaaa
     1921 taaatttaga aactatgact gcagttaagc tagaaatagc aaatataaaa tttgaaataa
     1981 tctaaatata aggggaaata caatgaatat atctttaaat gactataaaa tagccaatga
     2041 aatattttat agattactta aaaaaagtga gttaaaagaa aaagaagcta aagaattatt
     2101 tttagcttat ataaataaca gtaatgtaag gtatgcttta gaggcaattt gtgaagtatc
     2161 taaaataaaa ataatattaa tttcagatac cttatactta ataccaaata atgacaatga
     2221 tatttttggg tttgactata gaagagaatc tttattaggg aaagatttaa aagatagtta
     2281 tttaggatat ttgataatga caataatatt ttctgaattt acaaatgggg tagcaccagc
     2341 atcatatatt caagtattaa acttactgga tttagtacaa gaatccttag aacgttcaat
     2401 agctcgtgat aatctagaag aaatagaaaa atcatgtgag tttaatataa ctatcttaaa
     2461 gaactattgg gaaagtaaaa gaaagtggga cgaatctagt aagaaaggaa tagaaacaac
     2521 atcagcagat ttccaaatag ggtttattag aaaaataatt aatttcttaa aaaatgaagg
     2581 cttaattgta cacattgagg atgaagataa gataaaacca actagaagat ttcttgatct
     2641 tatgaataat tattttttag atgaagaaag gaaagtatta atagaaaaac tagttttaag
     2701 aaaaggggaa agtattaatg ccagtaatta ataaatacaa aattataaat tttaaatttg
     2761 gtatgggaca agaaagaata cttagcaaca gaaccatgga actgtatgga gaaaacttac
     2821 aagtagaagc agaaaataca gcaggcaaaa ctatgagtat tcaaactatc atacaaagta
     2881 tttgtccact atcggatgta gccaaaccgt ttactgcaat atataataaa aaagaaccat
     2941 tatactcaat gactgaatgg ttgctagatg atggtaaaac gttattatta acgggaatag
     3001 gatttgagaa aaaggctgga tcatctaaag aagaagggaa agaaaaaaag gacgatttat
     3061 acaaatactt tatgtttatt atagaagaat ctaaagaaat ggaggttgat atagataata
     3121 tacctttatt aaaaaacaat gacaaaggta taaggatagt acaaccttta acggcctcac
     3181 aagactatat aaaaatgcta agagataaat atggaaaaaa caaagtacac ttctttaata
     3241 attacttaag aaaacaacat agagaaaaat tagaagaata tggaattaac caaagtgaat
     3301 ggaaagaaat tattataaaa cttaatagta ataacaaaga aggtggacta tcagactttg
     3361 ttaaagaaca taatacttca gaaaaactta taagagataa aatattacct ttaatagaga
     3421 caagcttaac aacagaagaa ttcgctccaa tattgcaaat taaggaacaa gtaagaaagt
     3481 gtataaataa tattatagac atgaatgaaa aattatcaga ttataaaaac tatagagatt
     3541 taaacattag agttgaaagt ttacaaaata aaataaatag gatcctattc atagaagaag
     3601 ataaaaataa tgtattagga gaattagcta atctatatgt acatatagaa aataaacgca
     3661 atgaattaaa tgaagaaata aaacagtatc aaaaaatgaa agcatcaaaa gaagaggaat
     3721 ttaagatagt agaatatgaa gaagatagct atgagtattt tagaaaagaa aaagagatac
     3781 aagatactca ggtagaattt gataaagcca aaaatgaatt aaatgataaa atagaagaac
     3841 tagaattaga tgaacaatat ttaaatatat caaagttcaa aagaaaaaag aaggggctag
     3901 aagaagggga aaaagataag atagaaaaag aaagtgaaaa acaaagaaaa gaggtaaatc
     3961 aagaagaaat aacggctatt ataaatcgtc taggtagcag tattcattat tgtttaaagg
     4021 aagaaagtat tataaataat gaaaaactta aagaaaatac aaacaacaat atagctatac
     4081 aagaaaaaaa gaatcaactt aatgatgaca aaagtaagct cacaaaagta ttgataagta
     4141 aaaattcaga aattaaatat atagaaggca aagaacgtga ttttaataat caactacatg
     4201 aatttaaact taataacact gatattaaag gctttatttc taataatttt ttaggcgaag
     4261 taatagatta tactaggtat ataaattatt taaatataaa agtaaaatca atagaagaaa
     4321 atcttgaatc gaaagtcatg caaatagaag aatcgaataa acttaaggat gatataaatg
     4381 aagctataaa taatcttaaa ttagataatc atgaatctta tatgaaactg caagtattaa
     4441 aaactagaaa gaaagatata gataatttta aagaaaaata taaaatcatt aaaacaagat
     4501 ttgaaataga caatgaatta gataactcta ctaaactata taaagaggta aaacaagagc
     4561 ttaacaaaat gaaacttcaa gtacaggact tagaaaataa taaaaagcaa ttagaaaaag
     4621 agctagaaga tttacaaaat tataaagata taattttcga agaagaaata ttaacagatc
     4681 tagaaagaaa tggaataaaa cctattgaag gcatgaaata tctattaacc ttagatggag
     4741 atttagaata caaaaataat ctcatatcta aaaatccatt gctaccatat tctataataa
     4801 ttacaaataa agagtatcaa aaactgaagc aaataaactt aaaaaataaa actaaaatct
     4861 caatgcctat atttgtaaga gataacatag aagatacttt tataactaca tctagtaacc
     4921 ttgtaattag taatggatta aatatactat cttctttcga taacaaagct ttagatcctg
     4981 aaaaaagaga aaaggatatg aatctaatca aagaacaaat aaatagtatt cgagataaag
     5041 tggagcaata taaagcagaa attgaaatat tagaagaatt taaacttcta actaataaaa
     5101 ttgattttac agctgaagac ttatccatag atgtgaaaat tgaaaattat aaaaaaatta
     5161 tatttgaaat aaacaataaa attaagagta atgaaaataa tgagaagaat ttatctaata
     5221 aaattaaaga actttacgaa aataaagaaa agctattaaa taacaagaat atattattta
     5281 ataaattgaa ggacattaga aatatacaag atacatattc tataatttta gaaaataaaa
     5341 atgaaataac taagttaaaa acaagtattg aaaaaatcga agaagaaaaa aaagttatta
     5401 aaaaatcaat agaagatatt gatgttaata tatttaattt aaattcagaa aaagatagga
     5461 taaataggca tatagatgat acagaaagca agataaataa atttaagatt tatgaaccta
     5521 ttaaacttga aaatgctaaa ctagaagaat tagaaataaa atacaagaaa tacttagaag
     5581 accctacaaa aaaagagatt gaaacattaa attatgaaat ctcagtttgc ttaaaaagaa
     5641 tagagagtat tactatagag ttagaagaat ttagacaact tattagtagt gataattttg
     5701 aaaatatacc tatattattc acaatagaag aacttaataa aagaatagtt ttaaataaaa
     5761 gtaaggttga tgaactacga aaacttaaat atagtattaa aagcgacata gataaaaatc
     5821 aaggttcatt aaacactaag agggaggaga tacttaaaaa atttaatgga aaagaacctt
     5881 tatctactag cttaatagtg aactttaatt ttgaagaaag aaaaatagat ataaaaaaag
     5941 atataaagga tattggtgaa attatagaag gtaatagaac tacttgtcaa aatctagaac
     6001 aggaattaag agacttaaag aaatataaag tagaaagaaa agctataacc tatcaacctg
     6061 aagatatttt tattgaaagt aaaaaactac aatcaaaact gcaaaatgtt gaatataatt
     6121 taattaaatc tataaatagc tataaaaata caatattaga aataaaagaa tttaattata
     6181 agcacatgga aaaatacaaa aactttatag aaactttaaa tgtaaataaa gataattact
     6241 ctcgacaaaa agaacaagta gaaatagtat tactagttat aagagaagaa ataagcaaat
     6301 tagaaaaaca tagcgaacaa ctaagaaaag aaaagactat aatatcaagg caaataaaag
     6361 attatgttat ggactgtatt gatgaattta aaatcataaa taaacttggt aaacataaag
     6421 gacaagcctt attcaacata tcactaccta aatctgataa aatagaaaat agtttgagct
     6481 tagtagatat gctaatggat gaaattgtag aagaagcaaa cctaggtgat gttgaacaaa
     6541 aaataaatac cttttatatt ttaaataaga ctcttaatat agggagaatt cctgtatcag
     6601 ttataaaata tgaaataaat agaaatgaaa ctataaagtg gaactctatt aataacgaaa
     6661 caacaggagg acagagattt tgtatctcat ttattgtgac aatattgttg atggaatata
     6721 aaagatatga tagaaatgca atagttgatg aaagtaaata taagggtaag gtactactta
     6781 tggataatcc ttttggagaa acgtcgcagc aagatttcct taaagaaata tttgaacttg
     6841 ctaaaaaatt caacgttcaa attatatcct atactcatgt aacaaatgct tctataagag
     6901 caatgtttaa taagatttat ttaatgacag ttgaaaaaac tacttctaat aaagaatttg
     6961 tggatattaa tgagattaag caaaataaaa ctgatgaatt ggtgtatatg agtaaatttt
     7021 atataggtaa taaagaagaa ttaattcaag aggatatatt tagtttaata taaataacaa
     7081 gtaaacaaat aaagtgatta ctatcagtga ggcaggtaga aactgaatta ctttgtgact
     7141 cattacgact cttggggtaa aattgtgtat aaatgtgaag aatttcgtga ttgtcagtac
     7201 aaaagcagct ttattaaggg taataatagt aagactcctc ttgaaaatag gactaaaaac
     7261 ttacaagtat ctaagttatt tcatgaaaaa agaaaaatta aagagaatta taagaccaga
     7321 gggatgtgaa cttagagtga atagaagtat acaagtagaa agagctatcg cagaacaaat
     7381 ttaattttat catacgatag cctcttgtta ttttagtgat gaaatccatc aagcatacca
     7441 taactacaga aaaagaggct tttctgatgg tatattgggt tcttttagag tgtgtgattt
     7501 gctacatgtt cattaggata ataatagtca gtaaatgata ttactgatag aatagatata
     7561 ataaactaat ctagttccat attgtaatgt ttattaaaga gtatactttt atgattataa
     7621 tagctatttt ctttgaagct tattgtacaa gagatttgaa tatatcaaat ttggatatag
     7681 gtataattct cttagcagtt ttatttatac agtctttatc taaattagtg ttgtatatac
     7741 tttgataaaa gggggatatg taactaagtt gtaatattga tgaaaataaa aattaaaaaa
     7801 tatagagaaa aactccttat gatacaaaat gaacttgcta aatcagttgg ggtaagacga
     7861 gagactattg tacacttaga aaatggatta atgtaccata aaggtatcca agataaatag
     7921 ttaaaaaact agataagaat aaggagcctt tacagaacaa atttagtgtg ctcgtgcgat
     7981 agcccttttt ttataaaaat taatctaata acctatataa ttaaatttat aaaacatata
     8041 tgaaagggta aaactatatg gctaaaattg ataatatgat gtccatatta tggatgttaa
     8101 actcagacaa aaaagttacc gcaaaacaaa tatctgaaaa actagaaata aatataagaa
     8161 ctgtctacag acacattgat gcattatctg ctagtggagt acctataatt tcagatacag
     8221 gtcataatgg tggatataca cttttgaata atttcatcaa agcacctctt tttttcgata
     8281 cagaagaaca aactgcgtta cttcatgcag ctatatttgc taaagaagag ggaagtttct
     8341 tagatgacac actagacaaa gcaacctcaa agttaaaaat gtattcaaat caaaaacaag
     8401 aagaaacttt aacaaaacat ttaataggtt ttgaagtaat aaaaccaact ggaaaaatat
     8461 ccatagaatc tgtacttaaa aagttaaaaa atagtatagc aaatgaatta tctgtagaaa
     8521 tcaactatcg tacagctcat gaagaaaaat caaaatgtag gattataaat ccatatggaa
     8581 ttgtacgttg gaataataat tggtacatag tagcattttg tcaaatgaga aatgagattc
     8641 gtagttttag ggtagataga attagtagtg tcatagaaac atcaaatact tttaataggc
     8701 caaaagaatt ttcagcaagt gaatttttta taagagggat tatggatgaa acaaaagatg
     8761 aggataatct tatatcatta gttatagaag gaaagatgaa tgcattggat aacttatgtc
     8821 agcattggct tctaggatat tatttaaaag aaaggacttc aaataaagca atatttaaac
     8881 ttcaagaaga gataatattt acatatgtac cgaatatact tataccttat agaaaatcta
     8941 tacaaataat tgaaccatta agccttaggg ataaaatggt tgaagattta ctcgaattaa
     9001 ttaatcatta tcaaatttaa taacttaact gacagagtat gtcagttaag ttattttaca
     9061 attaggtata ttaattaatt atagaaggag caaaaaaatg aaaaagagaa aagtatatat
     9121 gtatgtattc gatactatgt cagattggga agttggatat ctaactgctg aattaaatac
     9181 aggaagatat tttaaaaaag gattaaaacc actaaaagta attacagtag gagttgataa
     9241 aaatcctatt ataacaatgg gtggattaaa agttttacct gagattacaa ttgacgagct
     9301 taatattgaa agtaatgact tattaatttt gccaggggga aatacttgga catctatagg
     9361 tcatgagaaa atattagaaa aatctaaaga ggctttggaa caaaggtcta tagttgcagc
     9421 tatttgtggt gctactttag ggttagctaa aacaggttta ctagatttta gaaatcatac
     9481 aagtaatgat ttagaatttt taaaaatggt gatactaagt tattctggag aaaaatatta
     9541 taaaatggaa tctgcggtta atgatgaaaa tttaattact gcttctggaa ttgcaccatt
     9601 agaatttaca gtacaagttt taaaagtttt agatgttttt aaagaagaag cattaagttc
     9661 ttggttgaac ctttataaga cgcatgactc taaatatttt ttcgaactaa tgaattgtaa
     9721 taaataaatt taagatttaa ttttaaattc aaattttaaa ttactttttc tatggcaata
     9781 gataagtagt ttagaatttt atcatatata taatttgtat ttataccaac acaaatagaa
     9841 atgatagttc caataataaa atttataatt agttccatat ggagtacatt tctttcctac
     9901 taatataact aagaatatat gaaaccacca tagattgtag ttttttctgt tgtattatat
     9961 catgggatag taaagtgacg tatatttttg atgaaaacct aggattgtat atgaaatgaa
    10021 ttaatcctgt gtattaatct tgagattcgt gtctaatgat ttgtaaaaat aaataatgaa
    10081 atacttagaa atacttgtaa atgctagtca atattggcta gtatttttta ttttagtaat
    10141 gaaatcccca actgtacttc atccacaaaa aaagagatat ttttgagggt gtattggagt
    10201 gaacttaggc tgtataatta tacatttcaa tatcttatat taattaggat aatagttggt
    10261 aaatgatatg attgaaacaa tagatataat aaactaatct agtactgtac tgcttactaa
    10321 agaatatatt tttatggtat aagaattttt tagagcgttg attctatagg agtgaataaa
    10381 aaagtaattt tataatatta tttgtaacca ttagatgtct attatgaaat cttttatgga
    10441 ctatatttgt aaaaatatta ttaagtattt acaatatttt cttgcaattt atttggaaag
    10501 gttatatatt tataacatga agtgttataa atatataacc tttaaggtgg attttatatg
    10561 tctaaatcag aaactggtgt aaaatcaaaa ctcatattgg aattacttgt aataacttat
    10621 ctttttatag ctgtattagc tactaagtat atttttaaag gtccaagtac tgcatctctt
    10681 ccaggactga gctggttaat agtctttgga tgctattcaa tttataataa aaaatataat
    10741 gatttagttg atgaatcatc taaatttata ctatcaaagg ttaatcagat tgcaataaca
    10801 ataatgatgt attctatagt tataatagct attttctttg caacacctta tataagtaat
    10861 ataaatatat caaacttgga tactggtata attcttattg cagttttatt tatacaatcg
    10921 ttatctaaat taattttgta tatatacttt gataaaaggg ggatatgtaa ctaagtggca
    10981 atatcgatga aaacaaaaat taaagaatat agagaaaaac tccttatgac acaaaacgaa
    11041 cttgctaaat cagttggggt aagacgtgag actattgtac acttagaaaa tggaaaatat
    11101 aatccttctt taaagttagc gatggatatt gctaaagtat tcgggaccac agtagaaaat
    11161 ttgtttgaat ttattgaaga agattagtag ataggatata aatatttata agattgttaa
    11221 taaaatatag ttatttaatt attaattttg ttaaaaatat ttataaataa tataatgtat
    11281 gagtaaagtt aggttgtaat tattttaaga taagaagact actgtaaatg ataaaaatta
    11341 gtgaatatac agccaattaa taaataatag ataaaacttt aaagcttaga tgtgaggatg
    11401 agatatggtc ttacagtagt tcacactttt aattaagact taaaatggaa accatacaca
    11461 tacatacact atttactgaa agtggactag ataaaaaaat aataagtgat ttaagaaagt
    11521 atctcatata ctatataagt attattttag gtgacctatt tataggtcat tttttatgta
    11581 tataaatgat aataatacta atatttaaaa taggttttaa agaaataaaa tatccagatt
    11641 gaaaaatgga ttaatgttaa taaaaaaatt atatgtataa aaataggtat aattatagaa
    11701 aaaaacaata ataaaataag ctgttataga aaaatacaat tatatagatt gttgttgaat
    11761 cattgactgc aagaataata acatttaaaa tgaacatgga gaatatgggg gaggaatcaa
    11821 tatgaagaga aaaatagcag ttgttttatt aactgtagca atgttattat cacttgtagc
    11881 ttgtagcaat gataacacat caaaaagtga taaattagag gatacattag tagtatatac
    11941 tacacatgga gaggatatgc taggagcaat ttctgaagaa tttacaaagg aaacaggagt
    12001 taatgttgaa tttataaact taaaaggtga actagcagac cgtgttagaa gtgaaaaaga
    12061 taatccgcaa gcagatataa tgtttggtgg agatacatct acatacatgc aattaaaaga
    12121 agaaggatta tatgaaaaga ctgaaccaag ctggaataat gaattagaca agtcttacaa
    12181 agattcagat ggatattggt atggaactat aaaaacacca gtaatgatgt tttataacac
    12241 aaaactatta tcagctgata aagcgccaaa agattggagt gatttaacaa aagaagaata
    12301 caaagactta atagttacta gagattcatt atcatcatct atgcgttcta ctattagtaa
    12361 cttaatcaat tactactcaa atactaagag tgaagaagaa gcttggaagt atcttgagga
    12421 tttaaataag aatataaaga attactacaa tagtggaagt gtcatgtatt cagcaatagg
    12481 taaaggtgaa gctacaatat ctgttgcagt attaagtgat ataatagaga ataaagaaaa
    12541 aaataatatg ccattagaag ttgtagatgc agagagcggt tctgtagtaa tcacagactg
    12601 tgtagcagct ctaaaaaatg caccacatcc aaatgcagca gctaaatttg tagattttgt
    12661 tggaagtgct aaggttcaag ctatggttgc aaatgatttt aatcgtattc caacattaga
    12721 ttctgcactt aaagattcac caaagtggat gtcaaaagaa tataaagcta tggatgtaga
    12781 ttggtctgta attggaaaaa atcagtctaa ctggattgaa aagtgggaaa cagatataat
    12841 agataacaat aaaactataa aatagcctaa ttaaataatt taatattata tacaaaaata
    12901 gtgaggattt tcctcgctat tttacgtata aggaaactat agtatctcat aattttgtat
    12961 aattcataca acaaaatgat actaatacat acttattaaa taaaatagtt tttgagtaac
    13021 tgatgaagta attgactata taaagtgatt tgtagacata gtctctcaaa taaagtgaat
    13081 tttttattat gcaaaacttt taattatagt aaaagtacaa tttatatggg caaggagata
    13141 aaaatggaac taaaaattag tgattataaa ttattatgta ataaaggtag tggcgctttt
    13201 aacgaagata tcgtaggttt atcaccttat ggggcatggg tacttgatgg agctacaggt
    13261 ttaaataata aaaatttagt ctctacagaa agtgatgcca aatggtatgt aagttggtgg
    13321 aatagttatc ttcatgagaa tatggaaaga gaagatagct taaaagtaat tattaaagaa
    13381 ggcattgagg agataacaaa agagtactac aaattaacag atgataaaga agtagaagca
    13441 atagatattc cttcttgttc atgttctata ttaaagtttt ataaagataa gatagagtat
    13501 ttacttttag gagattgtac tttattagtt aacaataatg aatctgtgaa aattttaaaa
    13561 gatgagactt tatgtgaact tgataaaaaa gtatttgatt ttatgaaaga agccaaagat
    13621 gctacaaacc ttactttaaa ggagagaaaa gccgaggtaa tgcgattata atagagaata
    13681 gacttaagaa aaatactgtg gggggatatt ggactttgga attttctaaa gaggccgttg
    13741 acaattgtgt aagtggattt attgatatag aatataaaat gaaaattatg atggctagtg
    13801 atggtttttc atgtggatat gatagatata agatttttga taaaaatgaa atgatgaaaa
    13861 tagcagagat taaaggtata gattatatat atagaaaggt tagggaacta gaaaaggatg
    13921 atgaaatggc aatagagttt cctagattta aaatctatga tgattcatca tgtgtatatt
    13981 tagagattta taaatattag ataatttatg gagacgatat gaagaatata gaattaaaag
    14041 aaattaatca ttattatgga aaagaaaaag tattacatga tattaatata tctattaagg
    14101 aaggagagtt ttttacttta ctaggtcctt ctggatgtgg aaaaacgact attttaagaa
    14161 taattggtgg atttataaaa ccttcttctg gaagtattta tgttggtgat aaaaacatta
    14221 ccaatttaga accagaaaat agaaatatgg gaactgtttt tcaaaactat gcactgtttc
    14281 caaatatgac agttgaagaa aatgtatctt atggattgaa aataaaaaag ctgtctaaaa
    14341 aaattataaa ggaaaaatgt gataattatt tagagctgtc aggaatgaaa gattttagga
    14401 aaaaaaagat tgatgaatta tctggtggac agcaacaaag ggtggcagta gcaaggtcat
    14461 tagcgactga gcctactatg cttttattag atgagccaat gtctaatcta gatatagctt
    14521 taagaatcaa gatgagagaa gaaataagag agatacagca aaagacaggt attactactt
    14581 tatttattac ccatgatcaa caagaagcct tagctatttc tgatagaatc gctgtaatgg
    14641 ataagggtaa agttcttcaa attggtgtgc caatagaagt ttacaaaagc ccattgaatg
    14701 attttgtagc aaactttgta ggtacttcaa actgtattgg aaaagaagat tatgttaatt
    14761 ttaatattga aaaagagaca aagccttata tttataaaag acctgaagaa atgatattat
    14821 tgcaaaatac aaatcaaagt ggttttgtaa aagtaaaagt agaatctaag aattttctag
    14881 gagcaatttt agaatataca gtatcaaatg atgggaaaaa atatcaagta actgagctta
    14941 atagactgaa taatagcaat aaattaaacg taggggatat ggcatattta ggtgtgctac
    15001 aaggtgacta atatgattaa aaaaataaac cgtagtaaat ttacatctaa aaatgttatt
    15061 tttattgttt tatacagtat attattttgg attttgattg gatatattat cataccaata
    15121 ggcaatactt ttgtacaagc attccaaggt aaaggtggat attcttttga agtttttaaa
    15181 gaatattttt caaatactaa taatttaagg gtagtaagca atactataat tttgggattg
    15241 ggttctgtgt tcgtatgtgg aattatggga atatcacttg ccttatatat gacatttgtt
    15301 tgtgataaac ataagaaaat catacatatt cttttactaa gtccaatgat gattcctgga
    15361 gtaattatgg taattgcatg tattcagatt tatggagaaa gtggaatagt tacaaaatct
    15421 atagaaattt tactcccctt tatcaaaaaa ccttatgatt ttagtggatt aaagggaatt
    15481 ttatttgtgg ttgcttatac tcagtatgta tatttttatt taaatgtata tgttgcctta
    15541 aaatatgtgg attactcaac agttgaagct gcaaaaggaa tgggagcatc aaagctaaga
    15601 gtattttttg atgtaatatg gcctgttatt acacctgctg ttataacttc tactattata
    15661 acttttgcat cagggattag ttctttctca gctccaaatc ttataggtgg tggttttaaa
    15721 gttttaagca ctcaaattgt taggtctaag gctaataacc atatggatat agcttcagtt
    15781 caagtaatta ctttattact aatggggatt ttatttatga ttttattaca gtattatggc
    15841 aaaaaatatg gagtagtgcc atccttaaaa tctcaaagtt ataaagttca taatagaaaa
    15901 aaagacttat ttacatttgt atgtaaggtt attattgaaa tacaaattat tatgattatg
    15961 cttcctatag ttactattgt gtatttatcc tttgtaagca cacattcaat tatgacaacc
    16021 atatttccta gagactttac actggaaaac tatcagatga ttttccaaaa atcacgtgta
    16081 ttacagccta tattaaatag tttcaaaatg tctttaatgg cagtagcagt aggattgata
    16141 attactgtac cagtatcata tttagtaatt aaaaaggata ctgcatataa tcgactagca
    16201 aaatttatta taatgttacc ttggagtatg cctgcaagcg ccattgcagt taacctaatt
    16261 aatacattta atcaaaaaag tatatttgct tttaatgaat ccttaatagg aggtttttat
    16321 attgtaccta tagcatatac aatttatgct ctaccattat tattttcatc caatgaggtt
    16381 gctatgaagt caataaattt aggactagaa gaatcatcaa ggagtttagg tgctggaagg
    16441 ataaaaacct tttataatgt aatagttcca aatatgatgc caggaataat ttcaggagct
    16501 atattagtat tcattcgtac aattggggaa tatacaatgt cagccttgct ttatggtgtt
    16561 tataatagac ctatttcaat ttctattgta actaatatgc aagaatttaa gataggagta
    16621 tctatggctt atggtgtatt agtaattggt atatgctata tagcattagg aataattttt
    16681 aaattagata gaaaaagatt tacataagat aacaaaggct aatttataaa ttggtgctta
    16741 gaaaaccaaa tcactttatt agctaaagat gaatttgaag ggtgacactt tggtgtttta
    16801 tgtaaagttt atggaagtaa aacattaaat aatcgtgagt atgattccta taaatcgtat
    16861 atactattga aatccaagaa aaggtattgg ctaaatagct aatatctttt taattttttg
    16921 actcccatat agcattaact attacatgag tatctttaaa tttatagtta aagtatctta
    16981 attgtttatt ataaaaatat aggatggcta aagcacatta aattaaaaca cttaaaaaga
    17041 gaagatgtac atgagaaata taattaacta tgatacagac ttctaaaaat tatttagtga
    17101 aaaggaactt agagtgtata ataaaagttc cttttcaata aggaaaacga ttgttatttt
    17161 ctgagcttca ttaccctgat attatttaaa tataaacaac aacaaaatta acaagaagta
    17221 aattcaacct aaaatttaaa atatcgggag gtacaatatg ttaatcaata tgaaggaaat
    17281 gctaaaagtg gcacaagaaa atcaatttgc tgtaccagca tttaatatag gaagtgggca
    17341 aatacttaaa gcagtagtac agggtgctaa cgaaaaaaat gcaccagtaa tacttgcaat
    17401 acacccaaat gagctaagtt ttctaggcga tagctttgta gttagttgca tagaagaagc
    17461 aaataagagt aaagtaccta tggtaataca cttagatcat ggggaaaata aagaacaaat
    17521 attaagggca ataagatgtg gatttacatc agtaatgatt gatggctcac atttaccata
    17581 tgaagagaat gtagctatat ctagggaaat agtagaaata gcaaaaggat taaatgtgtc
    17641 cgtagaagga gaacttggaa caataggaac aacaggaaca tcaagtgaag gtggtactga
    17701 tgaaataata tatacagatt caaaattagc taaggatttt gttgaaaaaa caggagtaga
    17761 cacactagct attgcaattg gtacagcaca tggtatatat ccaaaaggtt ttaaaccaga
    17821 gcttaaacta gatttattaa aagaaataag agaagtagta gatatacctt tagttcttca
    17881 tggtggttca tcaaatccag atgaagaaat agcacaagca gttaaactag gagtttgtaa
    17941 aataaatatt tcgagtgatg ttaagtcggc atattataag aagtgcagag aactattaga
    18001 acaaaaccca agcttatatg agccagatac tatatatcca ccatgtataa aagctgcaag
    18061 agaagttata gagtttaaaa tgaatctatt caatgctata gataagttaa agtgctttta
    18121 taaaaataaa aattaattat gaggtaacaa attaaaagga ggggagaatt tatgataggt
    18181 aaaatactac gaagcgattg tattgtacta gataaacaat tacaaacaaa aaaagatgta
    18241 attaattata tggcaaatca tttgtaccat ggaaattata taaataataa agaaaagttt
    18301 atagctgata ttaataaacg tgaaaaagaa tcatcaacta atatgggtaa tttaatagct
    18361 atacctcatg ctaagtcaaa tgtagtaaat caacctgtaa tgatgtattt aagaacagat
    18421 agagagatta cagataatga tggtcaaaaa cttaggtcat tttttatgat agcagttcca
    18481 gaatcaggtt cagatgaaca tcttaagatt atatctaaac tagctacata tctaatggaa
    18541 gaaaattttc ttaagcatct actaagtgca gaaactccat acgaaatatt aagtatattt
    18601 aataataaag ctgcagaatc aggagatttt agtaataatc aaaataaagg tcttatagtt
    18661 gggataacag gatgtccaac aggtatagct catactttta tggcaaaaaa agcattagag
    18721 gaagcagggc aagaacttgg atatgaagtt aaaatagaaa ctcatggagc tataggtgta
    18781 gaaaataagt tgactaaaga agatataaaa agagctgaat acgtaataat agcttgtgat
    18841 aaagaagttc aactgagtag atttatagga aagaaagtgc tacaaaagaa agtagtagat
    18901 gctaagaatg tttcattagc taaaaattta attataaacg ctgaaaatgg aatggctaaa
    18961 gaaattaaac caggtggaaa gactttagaa gattcaaaag aaatgcttca agggtcagag
    19021 acaaagggtg tttataagca cttaatgagt ggtgtatctg aaatgattcc atttgtagtg
    19081 ataggtggaa ttggtatagc catagcattt atgtttggta tatttgcatc agacccaact
    19141 catgaaacat acaaccctat agctggattc tttggacaac ttggaggcga ttcagcattt
    19201 aagttattta taccgatact tgctggtttt atagctaaat caatagcagg aagacaagga
    19261 tttcctcctg ctatgatagc tggttttatg gcagttattg gaggttcagg gtttataggt
    19321 ggtatggtag ctggttttgc aacaggatat atagctttag gagtaatgaa gataacagaa
    19381 aatttaccag atgtattaaa aggagtaaat gctgtattga tatgtccact tttgattgca
    19441 tttacgagtg gagctataat gttctttgta gttaatacgc cagcatcatg gttaaatgaa
    19501 gctttacaat cttggttaca aagtatgtct ggaactaata aagtattatt aggtgcaata
    19561 ttagcaggga tgatggcatc agatatggga ggacctatta ataaaacagc ttcagctttt
    19621 ggattggcta tgttttcaag taatatattt gagccatcag cagcattaat ggtaggagga
    19681 atggtacctc cattagcgat atcattagct atgttaatat tcaaaaataa gtttactaaa
    19741 gaagaaagaa agtcatctct accaactgct ataatggggg catctttcat aacagaaggc
    19801 gcaataccgt ttgcagctgc agacccatta agaatgattc cagcaaatat aattggttct
    19861 tgtataggtg gggcaatctg tatgagttta gatataactt taatggcacc acatggggga
    19921 atatttgtaa taccatttgc atgtagtgca ccagttgcat atatagcttg tatattgatt
    19981 ggtacagtaa taacagcatt gattataggg gtagcaaagc ctactataag aagtgaaaac
    20041 agtgaagttg aattacttgg tgcttaaatt ataaataata gtaaaaataa gtaatggaat
    20101 aacaacaagt atatagaata ataataagga tatttagaat cagttaacta cttctaaata
    20161 tccttaattt ttattttgaa attatattat ctattatatc aatttagtaa ggtgtatcat
    20221 aaattctttt atgatacaga agtatcttag taattttaca tagttagtaa atatgttaaa
    20281 atagtactta atataaacat agaaatatat aaacataggt agggagggct aagtatgctt
    20341 gtgtcgaggc aaattgaatt tcttaaattg atactaaacg aaaataaata tagacctata
    20401 aaatacttta aagataaatt aaatgtatct gataaaactt tacaaaaaga tttgaagatg
    20461 attgagaaat atttag
//
LOCUS       DAEPOG010000061        20246 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEPOG010000061 DAEPOG010000000
VERSION     DAEPOG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.061"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            152..973
                     /locus_tag="KR432_001829"
     CDS             152..973
                     /locus_tag="KR432_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH0640367.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            994..2190
                     /locus_tag="KR432_001830"
     CDS             994..2190
                     /locus_tag="KR432_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH0640368.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            2356..5754
                     /locus_tag="KR432_001831"
     CDS             2356..5754
                     /locus_tag="KR432_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0640369.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5764..7437
                     /locus_tag="KR432_001832"
     CDS             5764..7437
                     /locus_tag="KR432_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0640370.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7711..7905
                     /locus_tag="KR432_001833"
     CDS             7711..7905
                     /locus_tag="KR432_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBH0640371.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    8074..8307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8384..9781
                     /gene="asnS"
                     /locus_tag="KR432_001834"
     CDS             8384..9781
                     /gene="asnS"
                     /locus_tag="KR432_001834"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH0640372.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9954..11129)
                     /locus_tag="KR432_001835"
     CDS             complement(9954..11129)
                     /locus_tag="KR432_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0640373.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11548..12912
                     /locus_tag="KR432_001836"
     CDS             11548..12912
                     /locus_tag="KR432_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0640374.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12934..13425
                     /locus_tag="KR432_001837"
     CDS             12934..13425
                     /locus_tag="KR432_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0640375.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13698..14369
                     /locus_tag="KR432_001838"
     CDS             13698..14369
                     /locus_tag="KR432_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH0640376.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14454..15320
                     /locus_tag="KR432_001839"
     CDS             14454..15320
                     /locus_tag="KR432_001839"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH0640377.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15353..16855
                     /locus_tag="KR432_001840"
     CDS             15353..16855
                     /locus_tag="KR432_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH0640378.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16985..17437
                     /locus_tag="KR432_001841"
     CDS             16985..17437
                     /locus_tag="KR432_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH0640379.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17597..18400
                     /locus_tag="KR432_001842"
     CDS             17597..18400
                     /locus_tag="KR432_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640380.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18458..19345
                     /locus_tag="KR432_001843"
     CDS             18458..19345
                     /locus_tag="KR432_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0640381.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19364..20212
                     /locus_tag="KR432_001844"
     CDS             19364..20212
                     /locus_tag="KR432_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640382.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
BASE COUNT         7914 a         2169 c         3635 g         6528 t
ORIGIN      
        1 gataaaaatt aaatatttta gaggtgattt tatttagata tagaatttta gaataatact
       61 atacaaatgt attgtattat tatttttata tcaaaaatag taataaagtc tatatacatt
      121 tagaaaatac tgtttaaaag gagtaggatt tatgataaaa aaagagatat ctaactttag
      181 aggaagtagt gattatgtag tatcaccaga attgatggca tcagttaatg ttgctattgc
      241 tcttgaaaag ccacttttga ttaagggcga acctggaaca ggtaaaacta tgctagcaca
      301 agcaatttca aatgaattaa aaaaagattt agtaatttgg aatataaaat caactacaaa
      361 agcacaagaa gggctttatg tatatgatac tgttcaaaga ctttatgata gccaatttgg
      421 aggagaaggt gtagatgata ttagcaagta cataaaatat ggtaaactag gagaggcctt
      481 tagctcaaat caacaagtaa ttcttttaat agatgaaata gataaggcgg atttagaatt
      541 tccaaatgac ttgctttggg aattggataa aatggagttt tacataaatg agacaaaaga
      601 aactgtaaga gctaaacaaa gaccaatagt tattataact tcaaatgcgg aaaaagaact
      661 tccagatgct tttttaagaa gatgtatatt tcattatata gaatttcctg atagagacat
      721 gatggaagaa atagttaaag tacattttga taaagtagaa gaacacctat tggaacaagt
      781 aatgactact ttttattgga ttagaagttt aaaggacata caaaaaaagc ctagtacatc
      841 agagttaata gactggatac aagcattaac tttgagtgga atgcctattg aaaaaataga
      901 aaaagaagtt ccttttgcag gaatactact taagaataat gaggatattg aatctatgca
      961 aagacactta tagttaatag gagctggata attatgttta ttgaattttt ttatttattg
     1021 aggtcaagag gattaaatat atctttaaat gaatggatga ctttaattga agcattggat
     1081 aaagggctgt gctattcaaa cttttcaaat ttttactatc tttgcaggat gattttaata
     1141 aaaagtgaaa gtgattttga taagtttgat gcagtgtttt tggagtattt taaaggtata
     1201 gaacaccaag aagaaattcc agaagagatt atgaattggc ttaaaaaacc agatatagat
     1261 ttagaagagt ttgaaagatt ggaacaaaat ccaaatataa atcttgataa attaagagct
     1321 aaattggagg aacgaataaa agaacaagac tctcctcata atggtggtaa ttattggata
     1381 ggcacaggag gcacttccga gttaggacat tcaggaaaag ggcaaactgg tatacgtata
     1441 ggtggaaatt ctacttatgg tagagctgtt cttgaagttg caggagaaag aaaatacaga
     1501 gattttagag atgatgaagt tttaaatatg cgtcaatttc aagttgcact aaagagactt
     1561 cgtcaatttt ctactagaat agatgctcca aagacagagc ttgatttgga taagactatt
     1621 gaagagactt gtaataatgc aggatattta aagttatttt ttgagaagcc aagaaaaaat
     1681 acagtgaagc ttttattgct tatggattca ggtggttcaa tgagggggta tagcacttta
     1741 tgtaatacat tatttcagtc tgtaagtaaa tcaaatcatt ttaaagatgt aaagatatat
     1801 tatttccata actgtattta tgatagatta tttacaacac ctgaatgttg gttaagtaaa
     1861 tctattaata ctgaatggat attaaaaaat atagataaaa actataaagt tataattgtt
     1921 ggagatgctt ctatgtcgcc ttctgagtta cttcatattg gtggtaacta tcgtggtcca
     1981 tataattaca cacctggtat agaatggctt aaaaggttta agagaaagta cagtaaaatt
     2041 gtatggatga atccagaact tagagatgga tgggattcaa ttaattattg gtaccaaact
     2101 caaagaatga tacaaagtga atttaatatg tttccactga ctgtaaaagg tcttgaaaaa
     2161 gcattgaaaa atttaatgct ttcaagataa agtaaatata aatgatggta tgatttattc
     2221 aaaattgtac catcattatt tattttttaa tatatctatt ataaattgta taactgaaac
     2281 ttaaaaaatt atatagtcta atacatttta gacttacaca aaacatatac attaataata
     2341 attttagaag tcgaggtgat tataataaat tatgaattaa ttgtaaaata caatggagat
     2401 atattgagat tagaagaaga attaggtgta tcagtagaga tacttaattc atcatatgca
     2461 ataataacat caagtaatga agaagatgta aatacacttt taacttatcc agaaattgaa
     2521 tttatagaaa agccttttat attacagact caggatgtgc aaagtttttc aagtacagga
     2581 ataacggggt ttaaaaatag aactggttta actggaaagg gaactataat tggtataatt
     2641 gattcgggaa tagattatac tctacctgtg tttagagata gtgatgggag gtctaagata
     2701 ttatattatt gggaccaatc tattcaagga aatccaccag aaggttttag agaaggaaca
     2761 ttatatacta atgaagatat aaataatgca atagatggaa gtatgtatat acctatttca
     2821 acaactagtt tacatgggac acatgttgca ggtatttgtg caactattgc aagtgatgcc
     2881 agaataatag tggttagagt tggaaatata cagaccgata ttttttcgag aagtacagag
     2941 tttatgaggg caattaaatt tattttagat agggcattag aattaagaat gcctgttaca
     3001 ttaaatataa gttatggaag taatgaaggt tcacatagag gaacatcttt atttgaacaa
     3061 tatatagatg atatgtgttt attttggaaa aacaatatag ttgtagcagc tggaaataat
     3121 gcagataaag gaggtcataa aaggattagg cttcaaaata atattacaga agaagttgag
     3181 tttatagttg gggaaggaga gcgtatatta aacataaata tatggccaga ttttgttgat
     3241 gattttagtg tacatttggt aaacccatca aacaatcaaa cacaagcaat ctctctaact
     3301 tcaggtgaga taagaaatac tttgggtgaa actagaataa ctggttattt ttaccctata
     3361 gcaccgtact ctcttacaag aagagttact ctacaattaa gttcaaatac acagataact
     3421 cctgggttat ggaaaattgt atttgaacca atagatatag taactggtaa tgtaaatata
     3481 tatttgccaa cttctgaagg actaaataga aatactagat ttctgattcc tactcaagag
     3541 cttactgtaa cagttcctgg aacagcaagt agagttgtaa cagtaggtag ttttaattca
     3601 agaacagaca tagtatctat attttctggt gaaggagata cacaattagg tgtttttaaa
     3661 cctgatttat tagctccagg tgaagatata atatcttttt taccaggagg tacaagtgga
     3721 gcattaactg gaactagcat ggctacacct catgttacag gggtttgttc attgtttatg
     3781 gaatggggaa ttgtaaatgg aaatgattta tttttgtatt cacagaaatt aagggcatta
     3841 cttttaaagg gagcaaggag attaagtaat caatcttatc caaataattc atcaggattt
     3901 ggttttttaa atttatcaga tatagatttg tacactctat ctagtataaa tcaagattta
     3961 gaaacggagg atattggata tagaagtatt aataagtcat ttaaagatga ggaaaatagg
     4021 tataagttta tagatggtta taatatacaa atacataatg acttagagaa tgaaatttat
     4081 ataagtaaaa atgcaagtag acaatcagga atactatcag gaatagatgt agtccatact
     4141 cccgaatttg aagaagaact agctgggtta ggtatgagtc aaaatttttt taagataagt
     4201 gattcattag gtgtactatc tataaataat acagactata gtagtataca acgagttttg
     4261 caacttcctt caatcataag aactgtatcg actacaaaga tgacattact tggagaaatt
     4321 aatcgaggta cttttggagg tgtagttgca acagaagaaa tgggcgtaaa tttctttaaa
     4381 aataatccta atattaatat aacaggaaga gggactctaa tatcaattgc agatacagga
     4441 atagactatc tacatccaga ttttatttat ccagatggta cttcaaaaat agtatattta
     4501 tgggaccaaa caaaagaagg aactccacct gatggttttt atattgggac agaatataca
     4561 cgagaagata taaatcgagc tatcgcagaa aatgacccta gtctatctca ggatgaagtt
     4621 ggtcaaggaa ctatgttgag tggtatatgc tcagggcttg gaaatgtaaa tagtgaatat
     4681 gcaggtatag cagaagattc tgagctaata ataataaaac tcggtaagat agatggtttt
     4741 tataatagtg caatgttgtt tgctgcatct cagtatgcat ataaaaaagc ttttgaactt
     4801 agaagaccac tagttataaa tatgtcttta ggtaccagta gcttggcagg tttaaccaat
     4861 aggagcaata gtgaaaaagc attctttact agagggttat gtataacagc aggagcagga
     4921 aatgagggca atacacagac acatacatct ggaataatac cacatgttgg aggttctgtt
     4981 gaagttgaat tagagttaaa tgaagacgag gaagaattat cattagaact ttggttaaat
     5041 agaccagata aagctgatgt aataatagta tctcccactg gtgaagaaag taaaagtgtt
     5101 ggaatttcaa attataataa agtaacaggt ttatttgatt tagaggggac tgaatattct
     5161 ataacgtata tatacccaac aactttttct gggcagcaat ttactaatgt tactttaaag
     5221 aatgcaaaaa gaggtgtatg gaagattcga ttagtagggg tttatattat aacaggaaga
     5281 tataacttat accttccaaa tagagaactt ttaaaaagtg gaactagatt tagagaggta
     5341 gacccttttt acactataaa ttatcctgct attcaagatg atttaataac tgtaggagcc
     5401 tacaatacta ttaatggtag tttatggcaa agttcatcaa gaggaccaac tattgaagac
     5461 aggttaaaac cagatatagt agcaccaggt gtcaatataa tagctgctta tcctgggaac
     5521 acgtatgcaa ctataactgg tacagcagca gcaagtgcac atgcagctgg tgctgctgca
     5581 atgtatttcc aatatacttt tgttgatggt agatatccaa accaagcata tgtgcaaaaa
     5641 ataaaaactt ttatgcaagc tggtgctaga aaagattcaa acacagtata tccaaataca
     5701 aattctggat atgggctttt ggatgttaga ggaatgtttg atgttttaag ataggaggga
     5761 tttatggaaa aatcttattg tataatttat caaggtgata tagaatctgc cctacaagaa
     5821 aatggaataa ataggtatat ggttttaaat agtcagcttg cagtaatata tgtacctgta
     5881 gattttgatg aaactatttt aaataatata atacaagttg cttggtggga agaatctgaa
     5941 ccaatgagca gtctaattga aataactaat aatgtaaata atggagaaac cattacaaca
     6001 gcagctgaaa ctgattatat ttatgaaaat ccatataatg atataacagg aagaggaatt
     6061 ttattagcag ttatagattc tggtatagat tatttgcatc ctgattttat aaatgatgat
     6121 ggaactagta aagttctata tttatgggac caagaagcaa atacaaatcc acctccagaa
     6181 ggttttattt ttggaagtga atttacaaga agtcagttaa atatagccat aaatagaaat
     6241 gatggaagtt tgagtcaaga taatataggt actggaactt tagtttctgg tatattagct
     6301 ggaaatggaa ggataaattc gcagtataga ggtattacta cagaatcaga cttaatagta
     6361 gttaaattaa aatcatatac agatacttat tatgctggaa ggataaatta tagtgtttca
     6421 gattttttag cagctataac gtatgttact aatatagcaa gaacagaaaa taaaccacta
     6481 ataataaatt tgactattgg agttaagtca agtgctgtag caactacttc aatattagat
     6541 acttttaata tattgagtag tgctggtgtg gtagtagtga gtggtgcagg aaatcaaggg
     6601 aatacagata ttcattattc tggcagattt agcagtgtgg gagaagttca agatgtaata
     6661 atacaagatg gagatgacta tgctcttgat ataacactaa atacgaatgg tccagataaa
     6721 gtgggagcac agataatatc tccttcaggt gaagttagtc atgatataag gtattctcca
     6781 gacttttata tatatagagg aaaatttaac ttagaaaata ctacttatgc aatgagattt
     6841 atttatccat atattacctc tggaaaagaa aatttggaga taagattaag agatataaaa
     6901 cctggagtat ggattttaag attgacttca gaacttataa taagtggaga atatgacata
     6961 tacttaccaa ataaaaattt gatagctcca gacacaaggt ttttagaccc tgattcagta
     7021 gctactataa ctatgtatgc tgctagtgat gatgtaatta cagttggtac atttaataat
     7081 aagactgata gtatgtggat tggttcttcg aaaggaccaa ttagaggaag aggaattaag
     7141 ccagatatag tagcttcagg tgtagatata atatccacat ataaaaatgg aacatataat
     7201 acaggaacag gaacaggagt gagcagttct atagtcacag gtgttttagc cttattgatg
     7261 gaatacttag aaaaacagga taatgtaccg agattatcat tgtttacaca agttttaaaa
     7321 acatatttaa tattgggtgc tacaaaactt gagatatata catatccaaa tgtatcgcag
     7381 ggatatggga ttttaaattt aaaaaatacg attcaacaga tagcaaatac tctatagata
     7441 agaacctatg taaaattata attgattagt aatttaattg tatcaaatac tttagataga
     7501 gtagaaaaat ggcataaaaa ataaatatga aaattaaaaa aacagtttta tattttgaat
     7561 acacaaagta aatattttgt atataaatag gtataaatta agtattgaaa agatatgatg
     7621 ttaatttaaa aatcattata atttagactt atgaatttta tttgtaaaat tgctaaaact
     7681 attacaaaat aaataataga ggtgatagtt ttggaaagta aaaaggcaag agcattaaaa
     7741 ggtaaagata ataaaagact taaaagttca cttttagttg gaaatgagtc gacagcagca
     7801 tgggctgatg ttgaaaaatt aaaacctcat agtaaagttt caataccttc aattaagggt
     7861 gtcgaagaag caaaagactg ggttgacaat ggaagtaaat tataaaattt tggaaattta
     7921 agtaataaag aagtaatgaa gataagtatt tattatcatt atgcaaggta gaattattat
     7981 attttaaata acaatcagta tcaattatat aaatttattg actaaaaatt tataaattgg
     8041 tataattttg ttaattagta gaaataataa aagataaata ctgtgaagag aagagtagat
     8101 ataaaatgta gttctagcga gttgaggatg gtgaaagctc aatacgaggt ttatattgaa
     8161 gaacatcttg gagtttctaa tcgaaagctt atttagtaag tagacttaga cggaaggcac
     8221 cgttataggc tttaagcatt gcttaactaa gaggtcatta tgacaactag ggtggtaccg
     8281 cgagagtaat agtctttcgt ccctttatat agggatgaag ggcttttttt attgaaatta
     8341 tatttaaaag aaattaataa caattatact aggaggattt aaaatgcaaa aggaatttat
     8401 aacagtaaaa gaattgtaca gagaaaaaga acagtacata gggaagactg taaaagtagc
     8461 tggatggata agaacatcta gagtatctaa aaattttgga tttatagagc taaatgatgg
     8521 aagcttcttt aaaaatatgc agatagtatt tgatgagaaa gtagaaaatt ttaaagaggt
     8581 aggaaaatta gcaataagct catctatact tgtagaggga gagttagtat ctacagaagg
     8641 ggctaaacaa ccagtcgaaa tacatgcaaa aagcatagtt gtagaagggg aatcagatag
     8701 ttcgtatcca ctacaaaaga agagacatac gcttgaatat ttaagaacga tagcacattt
     8761 aagaccaaga agtaatactt tttcagctgt atttagagta agatcaatag ctgcctatgc
     8821 aatacacaaa ttcttccaag aaagaaactt tgtatatgca cattctccaa tcataacggg
     8881 tagtgactgt gaaggtgcag gagaaatgtt tagaataaca actatggatt taaatgatat
     8941 tccaaaaact gaagatggta aaatagacta tactcaagat ttctttggaa aagaggctaa
     9001 tttaacagtt agtggtcaac taaatgccga gataatggca ctttctttta gaaacgttta
     9061 tacttttgga cctacattta gagctgaaaa ctcttataca ggaagacatg cttcagaatt
     9121 ttggatgata gagccagaaa tagtttttgc agaccttgaa gataacatgg aacttgcaga
     9181 agatatgata aaatatgtta taagctatgt attagaaaat gctcctgaag aaatggaatt
     9241 ttttaatagt tttatagata aaggattgtt agaaagatta aataagatag taaactctga
     9301 gtttattaga ataacgtata ctaaagcagt ggaattatta attgaatctg gtcatgaatt
     9361 tgagtatcct gttgagtggg gatgtgacct tcaaactgag catgaaagat atataacgga
     9421 gcaaatatat aattgtccag tgtttgttac agattatcca aaagatataa aagctttcta
     9481 tatgagaatg aatgaagacg gtaaaactgt tagagcaatg gatttattag ttcctggagt
     9541 tggagaaatc gtaggaggtt ctcaaagaga agaaagagaa gatgtattgt tagatagaat
     9601 taatgaaatg ggattaagag aagaagatta ttggtggtat ttagaactta gaaagtttgg
     9661 tactgctact cactctggat ttggtcttgg atttgaaaga ataattatgt atatgacagg
     9721 tatgtctaac ataagagacg ttataccttt cccaagaact cctaaaaatg ctgaatttta
     9781 ataattatat ataataaaac ttatataact ttaagtataa acactaaaat gacttgcata
     9841 tttatctatt ttagagatat gcaagttatt tttattgtac tataaatata taatttgatt
     9901 acttttattt tgcgatataa tacttatagg tttatataat agtatgtaac tttttaataa
     9961 aatgcaacac aattacgtcc ctgttctttt gcagtatata gagccttatc acaattactt
    10021 ataaactcat ttaatgttat tgccttttca attttctttg tataaactcc aatactaaca
    10081 gttacacatt ttaaaggaac atcatcacgc tcaaaattgc aggactctat aatttttcgt
    10141 aaacattctg atttaagttt agcatctgtc tcatcacagt taaaaataca tataaaaaat
    10201 tcctcaccac catatcgtat agcatgttca ttttcagaac ttgtaaaatc taaaattact
    10261 ttggcaatat tttgtagaca atagtctccc ttttgatgtc cataattatc attaaattgt
    10321 ttgaaataat ctatatctat catcattcca gttataggtt cttgatttgc atggctgatt
    10381 aatttttttt gtagaatatc ttcaaggtat ctacggttgt tcattttagt aaggtcgtct
    10441 gtcataactt tgtcgttaag taaattattt atatgagata tttcttcata ttgttgacta
    10501 attaaaagtt tatcatagta agtaaataca cgatttctat atagcattgt agaaacaaaa
    10561 atggcaaggg atgttataaa aaatgtgttg acaatattac taaactcatt gcctggtata
    10621 gtttgaatcc caggtaagaa taaattaaga aacaaaaagc atcctccaaa aactattact
    10681 gattctccaa attttaatac tgagaaagct gcagctgcta acataatata actaaataca
    10741 cttaggtcat ttccacctaa ctggtcaagt agagtaatta cacaacccca taggcagaaa
    10801 aaagtcacat aaattatcca taaatttata taaaacgtac aacaatcctt ttttttattc
    10861 caaagataag aaaatacaaa cataaagaca gaagatacac aaaatagtgt tacatacata
    10921 taaaaatacc attggcgtct tggtgtggca aaagggcctc cttctctagc tgagattgat
    10981 atcatcataa ttatttgaga tattactatt attatgcaaa tgattttacc cgtatgataa
    11041 agataattgc agagttcttt ttcaacattt tctttgtgtt ttttgtctag aggaaatcca
    11101 aaaaattttt ttattttttc taagttcata taagcctcca ttaaaaataa agcataaaaa
    11161 taagtattcc tttctatcat tagatatttg gaatacttcg tttatgattt aattatatca
    11221 aaatatattg tgtgtaacaa tctaaaattg tgatagataa aatttattag ctaatgttga
    11281 gtaactttat taattaatac tttactataa ttaagattta cagatagttt gctgctgaaa
    11341 ataaagaata gaataaattt aaattaagca taataaaata taaataataa agttcgcatt
    11401 aacaaactat ttaaatatca agacctgtta aaaaacaaca gcaataaaca aataaaatag
    11461 ataaatatat ccaattaagg tataattaag ttttatatga tatagttata tttgataatt
    11521 tacaatagta aaacttggag ggaaaaaatg ggtgaaaaga aaataaataa tatgaattat
    11581 tcacataata attattccaa aaagacaggt gaaccaacta gaaaaataaa aaatagccat
    11641 atcactctta aaagtaaaga agaaaaaatt tgttctagtt gcaataaaga aaataatact
    11701 aaggataatt actgtaaatt ttgtggaaat gaactttatg aaatagcatc attaagacca
    11761 cttgaaacaa aactagactt gaaatctaaa ataaaagagc tttcatatca tgcaaataaa
    11821 agaggtgtat ttttaacaac atttacaacc atatttatat tgtttattat tgcattgata
    11881 tttaaagcta ttataactat tcaatttaat gatattagtt atttaataaa tccagcacat
    11941 ataatcctag ctctcaactt gggtcaaatt agtgtatcta tgtctaccat gatgggttct
    12001 gggtttataa atgccaatat aggtttgttg atattactta taatccctat tttggtatta
    12061 ttaatatcaa acctaatttt tatgagaaaa agatgtagag attcaaaaac tgtattagca
    12121 aattctctag gagttggaat tttttatgga ttagttttag ctattttatc tatttttact
    12181 aatgtaaaaa ctagttcaca tagtatgtta gaatatggat atgcattaga atatagttat
    12241 gaatttttta gtgttctttt aaatggattt gtattagggt ttatatgtac atacataact
    12301 acttataaaa aaagttatga aaaggaaaat atgtatttat ctcttttttc aaattctata
    12361 agaacattca tattaggtta tgttttagta ttagtaatac tattggtact aactatatct
    12421 gatagtagtt acttaaatga actagatatg agtagttatt caaatggatt aaatttattt
    12481 actatactcc cacaaatagc tagttatatg tgggcttttg caaatggaat ttcagtaacc
    12541 ataataaact caacagtatc aatgtttact ttaagttctt catctttatt tggagatact
    12601 aaattaatgt tttatgcaat gggagcattg tctatgttaa tattattatt aaatggatac
    12661 aagctaagat ttaagtataa tacagatagt ataagaccta taatagtatt tagtatatat
    12721 tatgcatttt tgatgggaat attggcttta tttagtacat ttatactaga tagtaatatt
    12781 aatttcttta atacaactaa ttatggaaca actttaataa tgcaatttaa agtcttacaa
    12841 gctattgtaa tttcatttgt atatagtttt gtgatatctt taataggata taagctaaat
    12901 tcagctgatt aaatatgatt ggagggaatt tctgtggaag gaaatactaa taataaagta
    12961 gatgataaat tttctaaggt taagaaaaag tttggtagtg gaaaatttaa agttgaatta
    13021 tataaagaaa aatcaaaatt aaatagagag attgatgata ttcaagaaaa gaaagcaaag
    13081 atattcttgg aaatgggaat actgacatat caaaaaatta gagaagattc tattaatgat
    13141 aatagttttg ataaattctg tgatgagtta ctagaacttg ataagttaat ttatgaaaaa
    13201 aatatggaaa ttaatgaaat ggaaatggca gaaagtaatg taacttgtga gtgtggttat
    13261 gttggaaatg taaatgataa atattgtgct gaatgtggta aaaaatttga attggaaaat
    13321 gaatgtgatg attttataat ttgtggttat tgtgaaagtg agatagatac agaggctgaa
    13381 ttttgtccat gctgtggaag aaaaattata atggatttag aataatattg ggaaacctat
    13441 gtatagtata catgggtttt tttattgaaa ttatttaaat ggacttataa cagtagttat
    13501 ataaaatgaa aataaacacc aaaaaaatga aataaaattt cttttaaatg taaaaaacaa
    13561 gctaatatat aaaataaatc agtaaaaata tagatgcgag aaaaaaatat gaaaaaatat
    13621 tgcaaagaaa aagttttcat gttattatta atacataaat caagaaaata aatttcagaa
    13681 acgaggtaat ttaaaaaatg ctagataagg taaagggaag attaatagta tcatgccaag
    13741 ctttagaaaa tgaacctctt catagtcctt ttataatggg gagaatggct aaggctgcta
    13801 tggaaggtgg agctgttgga attagagctc aaggagttga agatattatt gaaataaaaa
    13861 aagttactgg acttcctgtt ataggtatta taaagagaaa ctatgaagat tcagatatat
    13921 atataacgcc aactaaaaaa gaagttgatg aacttttaac aacagggtgt gaaatgatag
    13981 cattagatgc taccaataga gttagaccaa ataatgaaga cttaaaagaa cttataaagt
    14041 atataaaaga aaacggtgtt ctagtaatgg cagacatttc aaattatgat gaggctatca
    14101 aagcacaaga gtacggtgta gattgcgttt caactacact atctggatat actccatata
    14161 ctaaaacatt agaaggtcca gattttgtat taatggaaag attagttaaa gatttagaaa
    14221 tacctgtaat agctgaagga aaggtaaata ctcctcaaga tttaaaaaag gtctttgagt
    14281 tgggtgttca ttcatcagta gtaggttcag caataacaag accacaatta ataacagaaa
    14341 aatttgttaa agcaattgaa attaattaaa aattaattta taaattaaaa aattattata
    14401 tttaactata cttgaaaatt aaaattggag gtaaagatat gagaacaaca gatatgaagg
    14461 gaatttattc agcattatta gtttcatttg ataaggaagg gaatataaat gaaaaagggt
    14521 taagacaaat aattagacac aatatagatg tttgtaaagt agatggtctt tatgtaggag
    14581 gaagtacagg ggaaaacttc atgctttcaa ctgatgaaaa gaaaagaata tttgaaatag
    14641 ctaaagatga agtgaaagaa gaaataaaat taatagctca agttggttca gttaatttaa
    14701 aagaagctgt agaacttgct aaatttacaa ctgatttagg atatgatgca ataagtgctg
    14761 ttacaccttt ttactataaa tttgattttg aagaaataaa acactattat aatacaataa
    14821 taaattctgt agataataga ttaataatat actctatacc atttttaact ggtgttgata
    14881 tgagtttaga ccagtttgga gaattatttg aaaatgaaaa aataataggt gttaagttta
    14941 cagcagcaga tttctactta ttagagagaa tgagaaaaac attcccaaat aaattaatat
    15001 ttgcaggatt tgatgaaatg atgttaccag ctacagtttt aggtgtagat ggggcaattg
    15061 gttctacatt caatgtaaac ggtgtcagag ctagacaaat attcgaatta actaagaatg
    15121 aaaaaatatc agaagcactt gaagtacaac atgtgactaa tgatttaata acagatatat
    15181 taggaaatgg cttatatcaa acaattaaat tactacttga agaacaaggt gttgaggctg
    15241 gatactgtag acaacctatg aaagaagcaa ctgatgaaat gaaatcaaga gcaaaagaaa
    15301 tatacagaaa gtatttttaa taaaatactt ttagtatggg aggattagtc aaatggtagg
    15361 atttacttgg atagattttg caatactagt ggtttactta ttggcagttt tatttgcagg
    15421 attgttattt tcaaaaaagg aaatgaaggg aaaagagttc ttcaaaggtg atggaactat
    15481 tccgtggtgg gttacatcag tatcaatatt tgccacatta ttaagtccaa tatcattttt
    15541 atcattagca ggaaattctt atggtggtac ttggattttg tggtttgcac aattaggtat
    15601 gtttatagca atacctttga caataaaatt tttcttacct ctgtatagta ggttagaaat
    15661 agatacagca tatgaatact tagaaatgag atacgaaagt aaaggtctta gagtacttgg
    15721 agctttgatg tttataatat atcaaatagg acgtatgtct atcataatgt atcttccatc
    15781 agtggttcta gctggtctta caggaatacc tgtaaatgta ttgatagtag ttatgggtgt
    15841 aatagcaata atttattcat atacaggtgg attaaaggct gtcttgtgga cagattttat
    15901 acaaggtatg gtattaatag taggtgttac agggacattg ttctatttaa tagcaagtat
    15961 aaatggtggt tttggaacag taatggatac tcttacaact ggacacaagt tcttagcaag
    16021 taatgaagtt atgtttgata aaaatatact atctacaagt gcatttataa tatttattgg
    16081 agctggtctt aatacttttt catcatatgt atcgagtcaa gatatagttc aaagatttac
    16141 tacaacaact gatataaagc aacttaataa aatgacatat ggaaatggtg ttttatcaat
    16201 gggacttgca acagtatttt atctaattgg aacttgttta tttatatact atactcaaaa
    16261 cccagattta gctcaaacag ttcaacaaga ccaagtattt gcatcatata tagcttatga
    16321 attaccagtg ggtataacag gaatactact tgcagcgata tatgcagctt ctcaatcaac
    16381 tttatcgaca gggcttaatt cagttgctac aagttggact ttagatatac aaactatcat
    16441 aacaaaagat ataagtatgg aaaagcaaac taagatagca caatatatat cattaggtgt
    16501 aggtatactt tctatagctg ttgcaatagt attagcaaat ggagaaataa agtcagcata
    16561 tgaatggttt aatagtttta tggggctagt actaggtgtt ttagcaggaa tatttgtact
    16621 tggtgcattt tgtaaaaaag cgactaaaat gggagcatat gttggattca tagtatcagc
    16681 aattttagtt gtttatttaa aataccgtat gccagaagta acttcttggg catattctct
    16741 aataacaatc acgacttctg tagtggtagg tacaatagtt agtgcaatag aagctaaagt
    16801 taaagcaaaa gtatctttac caaaagctga gactactgtt tattatgaaa aataatattt
    16861 aaatgataaa ttgtcttata gagtgattag ataaataatt tagttactct ataagattgt
    16921 ataatatact ttgtatataa aagtataaaa atagacttta taattatata gggagatagt
    16981 gcttatgata tttggaaata taaatcatag taagacatat tcaacattgc atgaagattt
    17041 acttagatgt tttgaatatg ctaaagataa ttcattaata gattatgaaa aaggtactta
    17101 tacaatagat ggtgatgata tttttgtgaa tattgtagag tataaaactt gtgaaaaaga
    17161 agatagattc tgggaagctc ataagaagta tatagatgtt catttgatgc ttgatggatg
    17221 tgaacgaata gatataaatt ttatagaaaa tttagaacaa aaaatatatg aagaaaaggg
    17281 cgatttcttg cctcttgatg gtaaaaataa tggatacatg gaacttagaa aaggtgattt
    17341 tttagtttgt tatccagaag atgctcatat gacaggaata aaagttttag aaaaagaaaa
    17401 tatcaaaaaa gctattttta aggttttggt tagataatga taatttatat aaccactagg
    17461 gctgaaaata gtgatttaac tttcagcgat tagtgagact tcgtaaattt tagttagatt
    17521 actattttca gccttagttt tttatgagaa aattgaaaat aattaaaagc ttaaaatttt
    17581 gtaaaggagt taacttatgg aaaatataga tgtattctta ttaataggac aaagcaatgc
    17641 tagaggattg ggaaatccaa aagagagcat cattccaaat gaaaattgtt ttgaatattt
    17701 gagtactgat gaaattataa atatgagatg tgagctagag acttcagaag gtgatggtac
    17761 tatagcacct gcattttcaa atgaatggaa taagcttact ggaaataaag tttgttttat
    17821 tcacaatgca aaagatggtt caagaataaa aaactggaat catgataata actggtttct
    17881 aaatgacact atagaaaaat ttaatacagg ctgtactacc ttaagtaaac attataatat
    17941 agaaaataaa tacgttattt ggattcaagg tgaaagtgat gcaaaatatg gtagtgatgc
    18001 attgtactat aaagaaagtc taaaaaaaat tgcgtatagg cttaaagaag aatgtatgat
    18061 tgataaaatg tttgtaagtt taactggtta ttggcttgga gaagatgaat attttattag
    18121 aacaagacga attgcagcag ctcaagaatc tgcttgtaat gaatgtgata tactgtgtgt
    18181 aggaagtaaa attgctatgc aatttcatga cagaggatta actattgatg atgttcatta
    18241 cactcaagaa ggtcttaata tgttaggaga ggacttatgt aaaaatattt ataagtatca
    18301 tataaccaaa gaaaaaagtg tattaagtga tactatagac ttatcagaag caaggaaata
    18361 tatctgtgaa ttggaaaaga taagtaaaag atttgtgtaa tataaaattt taaatagagt
    18421 tgttaattta taaaaataat ttgtatggag atatgttatg aaaaattacg tttgtataga
    18481 tgttggaggt acttcaataa aatatggttt tattagagaa gatggattta taattgataa
    18541 atcaagtctt gatactgaag caaaggaaaa aggtggagaa ggaatattag ccaaaataaa
    18601 agatatagta aaaaaatata tagaggaaaa tgaaataagt ggtatatgta tatcaactgc
    18661 aggtatggtt gacccagttg aaggtaaaat actatttgca ttagaagaat taattccaaa
    18721 ttataagggt atgcaattaa aaaaagaagt agaaaaagag tttaatataa aatgtgaagt
    18781 tgaaaatgat gttaattgtg ctggacttgg tgaagtgtgg cttggcgcag gtagaggtgc
    18841 aacatcttct atatgtttaa caataggtac tggaattggt ggatgtatta taataaacaa
    18901 taaactgata aatgggttta gtaatagcgc tggtgaagta ggatatatga atataaatgg
    18961 aagttctttt caagaattgg catctactag cagtctaatt aaaaaagtag caaaaataaa
    19021 aaatttgaat gaaaaggact taaatggaaa aataattttt gatttagcaa agaataatga
    19081 tgaagattgt ttaaaagaac tcgataatat gataaaatca ttagcagttg gaatcgcaaa
    19141 tatatgttat gtaataaatc cagaagtggt aattttaggt ggtgggatta tggcacaaga
    19201 aaagttccta aaaccaaaaa tagacaaagc attaaaggag gttcttatag agagagttta
    19261 caaaaacact aatatagaat ttgcaaagag acaaaatgat gcaggaatgc ttggagcatt
    19321 atataatttt ttaaacaaaa tataaagaat agaagggttt ttaatgagta ttttagaaca
    19381 attggaatca ccaactttta aagtcacaaa atcagataaa ttattaatag cttatataaa
    19441 agaaaatata gatgatgtat tttataaacc aatatcacaa atagctaaag agagcaatat
    19501 tggtgaagca actataacta ggtttgtaaa aaaaatgaat tttaatggat tacaggattt
    19561 taaagttact ttagcacaag aaatatcaac tatcaataaa aaaaatatta taaataaaaa
    19621 tatacaaaat gatgagccag ctttggatac agcaaaaaaa cttttaagtt caaatgtaac
    19681 cactttagag aacacagtag agataatcaa tagtaaggat gtacatgact gtgcaaggct
    19741 tataataaat gcaaagaaag tttacttcat aggcattgga tattctggta taatagcaca
    19801 ggattcaaac tataaattta tgagaatagg ccttaattgt gtaagttttg atagcagtca
    19861 tacgatgata atgatgtctt ccataatgga agaaggagat ttaataattg caatttcaca
    19921 ttctggtgaa acagaagaga ttataaaaac tgtaaagctt gcaagagcca ataatgctaa
    19981 aattatatca attactgaaa ataaaaattc tgaattaaag gatatttcag atgtacactt
    20041 atcttatgta tcaggagaaa cagtactgga aactggttca atatcttcta aattagcaca
    20101 atttttcatt attgatttag tatatacaca ggtagtaaaa gaactttcta atgaagctat
    20161 tgaaaggaaa attaaaacta caaatgctat aaaattgttt aaaaatgaat aaaataagaa
    20221 atttgtaatg ttttatagag ctctct
//
LOCUS       DAEPOG010000062        19849 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEPOG010000062 DAEPOG010000000
VERSION     DAEPOG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.062"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..453
                     /locus_tag="KR432_001845"
     CDS             <1..453
                     /locus_tag="KR432_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0640383.1"
                     /translation="SVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYIL
                     DENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKI
                     FDVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            977..1387
                     /locus_tag="KR432_001846"
     CDS             977..1387
                     /locus_tag="KR432_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0640384.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            1876..2028
                     /locus_tag="KR432_001847"
     CDS             1876..2028
                     /locus_tag="KR432_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640385.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            2129..3805
                     /locus_tag="KR432_001848"
     CDS             2129..3805
                     /locus_tag="KR432_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0640386.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(4283..4921)
                     /locus_tag="KR432_001849"
     CDS             complement(4283..4921)
                     /locus_tag="KR432_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640387.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(5331..5507)
                     /locus_tag="KR432_001850"
     CDS             complement(5331..5507)
                     /locus_tag="KR432_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640388.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(5561..5657)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6453..6854
                     /locus_tag="KR432_001851"
     CDS             6453..6854
                     /locus_tag="KR432_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBH0640389.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            6851..7918
                     /gene="rsgA"
                     /locus_tag="KR432_001852"
     CDS             6851..7918
                     /gene="rsgA"
                     /locus_tag="KR432_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0640390.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            8454..9152
                     /locus_tag="KR432_001853"
     CDS             8454..9152
                     /locus_tag="KR432_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640391.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            9254..9802
                     /locus_tag="KR432_001854"
     CDS             9254..9802
                     /locus_tag="KR432_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640392.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(10203..11159)
                     /locus_tag="KR432_001855"
     CDS             complement(10203..11159)
                     /locus_tag="KR432_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0640393.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            11862..12857
                     /locus_tag="KR432_001856"
     CDS             11862..12857
                     /locus_tag="KR432_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0640394.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            12835..13698
                     /locus_tag="KR432_001857"
     CDS             12835..13698
                     /locus_tag="KR432_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0640395.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            13695..14489
                     /locus_tag="KR432_001858"
     CDS             13695..14489
                     /locus_tag="KR432_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0640396.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            14774..16153
                     /locus_tag="KR432_001859"
     CDS             14774..16153
                     /locus_tag="KR432_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0640397.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            16608..17321
                     /locus_tag="KR432_001860"
     CDS             16608..17321
                     /locus_tag="KR432_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640398.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(17593..19131)
                     /locus_tag="KR432_001861"
     CDS             complement(17593..19131)
                     /locus_tag="KR432_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0640399.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         7318 a         2283 c         3119 g         7129 t
ORIGIN      
        1 tcagttcatt ctatagaaaa tttgaataat gatgaagaat atttgaaaat aaatgataca
       61 ttagaagaca tattaaaaaa gtatgaaata ttagcaaatc aaaagaaaat aactgtcaat
      121 aattatatat tagatgagaa tgtttatata ggaaaaacag ctttaaagat tattctatct
      181 aatttgatta gtaatgcggt aaaatatact gatgtaaatg gggtaattaa tattgggata
      241 gttaatgatt ggttatatat agagaattca tacggtaaca ataaaatttc gaatatggat
      301 aaaatatttg atgttaaatt tgatttaaat aaggaaaata gtaatggatt aggtttatat
      361 attgtaagta atattctgaa taattacaat atagaatata aagcattgca ggatgaagaa
      421 ttttttatct ttaaaattaa aatattttct taaacaagat ataattttgc aatagttatt
      481 gaaaaataga gacttattat tagccatgga agtaaaggct tttttcttat tgaaagtgaa
      541 cagaaaataa ttgatacaag aaatcaggat ttgaacacgc taatgaaaat attgttagta
      601 aaactatatg agttagtctt ttcggatgca gagactgttt aaatatttga tagaatagaa
      661 acttaaaaag tttaataaaa ctcaattata taagtagtag atgaatatga tggttattta
      721 gattattgat aaaatggagg aaataaatat ggaagcatct aaacgtttaa tgttagaaat
      781 atttagaatt caatacattc ctctttttta taatatgtat agtagatgat tgtttcaggt
      841 gcgaattgaa acaatcaagt tcatataaag aaatattttt attctttcat ttcagaggac
      901 ttcttatgcc aagatgcaga agtcctcctt ttttatctga aaaacaagca gataaaaaag
      961 agatggagga aactttatgg caaaagagta ttatctttat atcagaggac aaaaggtaaa
     1021 agtcagtgaa gatatatata tagtctactg gcgagaaaaa gaacacgaga agtatttaga
     1081 gcaggtggac aggaaaaacc acttgctctt tttttcagca ttggatcatg acggacactt
     1141 tacagataat attgttgatg aaagtgttga tgtagaaaag attgttgaaa cacagatgat
     1201 gattgaagca gtaagaagtg ctataacaag gctcaatgca gaagaaagag atattattga
     1261 acgcttgtat ttcaatgatg aaactgttcg ttcagtagca aagctcaaaa gtattacaca
     1321 tccggcttta atcaaaagaa gaaacaaaat tcttgaaaag ctgaaaaaat ttatcgaaga
     1381 actttaaaac tttggtaacc aaagggggca aattttcctt atgtaaagtg aaggggagtt
     1441 tgacctcctt tactttcttg agtaggagat acgcctgctt atggcaaata ggaaaatgta
     1501 aagaaaacag tccctttcaa atattgatct ttgacaactg aatagaccaa ggtaggactt
     1561 tatctactta ctgagagtta tgacctacgc caagactttt ctgctgatga aaatcggttc
     1621 aaatgaatac cggcataatg ccgaggttag aagaaaatga gcgatagata ggaatacata
     1681 aaagataagc gtggcagctt atttcgatga tgtaagtggt gataatgata cttcaatagt
     1741 ttaagctgcc tcgtctgaaa caaggggcgg aggtgagatt cctatgtagc agccaatgca
     1801 cctaccaatg tcataaaact tagtaagaaa aataatattt ggttataaag aatttaaagt
     1861 ggaggaggtg tattagtgga taatgactgg aatggattgg ctgatttgat agcaaatctg
     1921 attacaaaat atgctggagt tttagattta gagaatcttc caaatcctac tccagtaaaa
     1981 aatacagagg ataagaataa atttgatata gctaaaagca cggattgaga aagagaaata
     2041 aaagtgctat aatatacttg agattaaagt ccaaaccttt attggtaaaa atatataaat
     2101 atataataag gatattgagg ttaatgctat gtcaaaagaa aaaataaaag tatatctcta
     2161 tacaagagta tctacatcca tacagataga tggttattct ttagaggcac aaaaatcaag
     2221 aatgaaggct tttgctctct ataatgatta tgagattgtt ggtgaatacg aagatgcagg
     2281 taagtctgga aaatctatag aaggtagagt tcagtttact cgtatgatgg aagatataaa
     2341 atccggaaag gatggagtgt ctttcgttct tgtatttaaa ctatcgagat ttgcaagaaa
     2401 tgctgctgat gtactttcga ctttacaaat aatgcaggat tttggagtaa atcttatttg
     2461 cgttgaagat ggaattgatt catctaaaga tgcgggtaaa ttgatgattt ctgttttatc
     2521 ggcagtggct gaaatagaaa gagaaaacat tcgtgtccaa acaatggaag gtcgcattca
     2581 aaaggcaaaa gagggtaaat ggaatggtgg atttgctccg tatggttatc aacttattga
     2641 tgggaaattg ataataaatg aggaagaagc aattgcaata agaactattt ttgatcaata
     2701 tgtaaataca agtattggag ctaatggtct ttctaaatat ttagaaaatc atggtataag
     2761 gaaaatacca agacaaaatg ggaaaaatcc attgtttgac gcaggtctta taagaaaaat
     2821 attaaagaat cctgtgtata acgggaaaat agcatttgga agaagaactt tagaaaaagt
     2881 tcatggtaca aggaatgaat acagacaagt agagcaggac gattatttag tagcagaagg
     2941 cattcatgaa gcaataatcc cagatgaatt gtggcaggct gcacaagtta aactgaaagc
     3001 acaagctaaa aagtatgaac atgtaaataa gggcaagaac atgaggacac acttattatc
     3061 aggaatagta aagtgtccga tatgtggagc tggaatgttt gggaataaat ctataaaata
     3121 taaaaaagat gggacgaagt ataaagactt ctattactat ggttgtaagc atagacttat
     3181 gaatagagga cataaatgta cctataataa acaaattaga gaagaattgt tagatgatgc
     3241 cgttgctgag gtaataatca agttagtaag caaccctaaa tttgcatcta tgatacaaga
     3301 aaaaattaac atgaaggttg atacatctgc tattgaaaat gagatagata attaccaaaa
     3361 tgaactaaga aaaagtcatt ctacaaagta taagcttatt gaggaaatag ataatttaga
     3421 tgtagaggat aaacattata agagaagaaa aaatgattta gatgataggc tttatcgtat
     3481 gtacgataaa attgaggaat tagaaagtca gttaatagaa gctaaagcaa aaaaagaaac
     3541 gatagaggtt gagaaactta ctggtgataa catatataaa gtattgattt attttgacaa
     3601 gctttataaa gtcatgaacg atgttgaacg caggcagtta atagaagcac tcatttcaga
     3661 aattcaaatt tatgaagaaa agcagcctaa tggacaatgg ctaaaatcca ttacttttaa
     3721 gctacctatc attgatgaag atcttaatat aagtttggaa aatgatgagc aagttgaaac
     3781 tgtagctaag ataatcaagc aatagtaatg tttatataga ttatatacta ataatatatt
     3841 gtatttgcca ccgatttgcc actgtaaagt aatttcggtg gcaaatttag tctacaatgt
     3901 tatcaaaaat ctctaaagtt ttatttttaa ttctataaaa cttattataa ttgtaatttt
     3961 aaaagaaatc gtcaatctac agttgccaga ttttatacaa cttatttttt tcataggata
     4021 atttacataa tctatagttg aatattattt aatattttta tattataatt cattagcaaa
     4081 tataggcatt acaaatgttg ataaacatgt gaaagcacat atccacttat aattattttt
     4141 ttaaaaacca taaaatcacc tcttcatttg ttgtatgttt ttataacaaa tgaggaggtg
     4201 atttccagac aatttaaaag gtttttgtag attgactaaa atttattttt attatttata
     4261 attcttaata atacatatgt acctactgat tccatattaa actgtataat ttttgttcat
     4321 ctttttttat aattaaaact acatagtttt cataatttct cttatatata tacttatcat
     4381 cttttttaac ttctaactta tattttttaa aatcttcttc aattttattt ttattacttt
     4441 tattaatgtt aacagaatct cttttaaatt tatcaatata tttttctatt tcattaatat
     4501 tatctttgct tacaactata ccacctttta aattaaaaac ttttttgaaa ttcttttcag
     4561 aaaatccttg aataaaataa ctttgtccat ctccattaat actatcttga gtatcaaata
     4621 cttcaatttc tttgttaggt attggtatat caaaattcca agattccttt agcattgaaa
     4681 tattagctgc ttttactaaa aaaagattat ataggatact tcctaataac aaaacaccta
     4741 ttaatagtag tgtgattaat acaatttttc tctttttcat ttttcgttca caaccttttg
     4801 aaaaaacatg atttaattca tcatttgaag gcatgtcttc aagaatagtt tttgttgtga
     4861 taggaatgct ttcttgaaac atatcatttg taatatcaaa atcttttatt tgattactca
     4921 atatccattt cttctaatat ttcttttgat tttttctagt acattgaata ctcttatata
     4981 cattctcctc ttttaaaatt aaaagttata aaagaataag ttatataaaa aattattata
     5041 ttttttaata tagcttattt gcaaatttat aattttgtta atctaatcct tcaaactaaa
     5101 aaatcattta tgtttacaga atttcatttt aagagatgtt tattttcaca tagataagag
     5161 aatttaagtt ctcatattta ttatttgata caaatttaca tctttattat atgtatttta
     5221 aatagaaaat aaaattatga atactagaaa tctatcttaa ttagctttct aacataaggc
     5281 tatttaaagt agataaaact aaaaagggtt ctctagaaaa ccaaatctat ttaattatac
     5341 ttacttacta ataccatcac ttgatgcaaa caagattcta ccacattttc tccgtactct
     5401 agtataaaat catctagttg tgtatctata aaatcacaat aatcatatga actaccaata
     5461 taagcagtca acatatgtgt acatctatcc attttatctc taaacatata tattattatc
     5521 cccctaaaaa actttcataa aaacaaaaag aatatctctt tttggcaact ggctgacata
     5581 actcatagtt tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag
     5641 ttttgccgat ttagttttat tctacaatta caataataca atagaattgc ttattattca
     5701 atatacttat ttgaaaatta aataaattta caaatatgaa cgttatttta tataaaaaaa
     5761 atagactatt catgtagaat atatggaaaa acttgattgt aggttttgta tgaaaagaca
     5821 gttgttatta atttaagtga gataaattga gcaataggca taaaatgaga aaacaaataa
     5881 tagcaattaa tttttattat taatactagg tgaacattaa attttaaaat accactagat
     5941 tttatagaat aagaaaatga gacaaacata aatatatgaa tatattaaat tatacttgaa
     6001 catacttata ttttatgata aaattttaat aataattatt agtgaaggag tgtcttgcct
     6061 gatgtcagag ttatatataa tggtaaccaa caaataaata gttggagacg catatgggtt
     6121 ctgtttaatc agaattttta agtgtatttt tgatacattt gattgttgtg tcttactctg
     6181 cctttataga taactaacat tagacaacaa ataataatat tacatattac tttgttaaat
     6241 tgtggtgtag tgggctttaa tctgctacac ttttattaat tttacacagt attattttga
     6301 atataattat gttgaaatag aagatactaa attttctagt ccacaggggt tttctgtttt
     6361 tgtgtaggta aatatgtata ccacataaaa aggcagatat aaatacctgt ggactttttt
     6421 tgtagaaaat acaagatacg gaggaaataa aaatgaatta taaaaataga aaacgagctt
     6481 attatcagga aaattcctgt atggataact ttatttgcaa aaattgtggt tgccttgtag
     6541 ttcctgaagg agctggtaca caacatagaa atcattgtcc acattgttta catagtcttc
     6601 atgtagatat cattcctggt gaccgtgctg cagattgtga tgctaatatg gaacctattg
     6661 gagtgtgggt gagaaaaaat ggtgagtggg caattattca tcgttgtaca cgctgtggac
     6721 atcttagttc taatcgtatt gcagctgatg ataattcagt gaaattaatg tcaattgcat
     6781 taaaaccact ggcacagccc cctttcccac tagaaagaat cattgaaaag ttggaaaagg
     6841 aggaaacaga atgaatggta tgaaacaaat caatttacaa gactatggtc taaataggaa
     6901 ttttattact caaagtatgg aatatcacga tttatacctt gctcgaatta tctcgcagga
     6961 aaagaacttt tatcaagctg tatgtgtaaa tggtgtactg tttgcagagg tttcaggaaa
     7021 actgcgtttt gaggctacta tacaatctga ttttcccaca gttggtgatt tcgttatgtt
     7081 ggatcgttgc aataataaaa atggtaatgc tattattcat catgtgcttc atagaaaaag
     7141 cttgtttgtg cgtaaggctg ctggaaaggt aaatgaggaa caggtggttg cagctaatat
     7201 tgacattgtt tttttgtgca tgtcactgaa taaggatttt aacattcata gattggagag
     7261 gtatctgtct ttaacatggg aaagtggagc tatcccagta gtagtactta ctaaatcaga
     7321 cttatgtgac aatgtgcatc gtaaattaca agtagttaat tctatagcct tgggtgtaga
     7381 tgtagtagtt acctcatcta tagagccaga tggttataaa cagatttcta agtatatcaa
     7441 atcaggctgt actgttgcat tacttggctc ttcaggagtt ggaaaatctt cattaattaa
     7501 ttgtctgcta ggaggagaat ttttaaatac aaaggaaatt cgcatagatg atgataaagg
     7561 aagacatgcc acgactagac gagagctatt tttaatttca ggtggtggta ttgttattga
     7621 cacaccaggt atgagagagt tgggaatgtg ggatgtttca gaaggtttag ataaaagttt
     7681 tgcagatata gaagcttatt tcggaaaatg tcattttcgt aattgtactc acacctcaga
     7741 accaggctgt gctatctggg aagctattgc tcatcaagaa ctttcgccta aaagatggga
     7801 atcctatcgt aggcttaaaa ctgaagcagt atttgcagac aataaaagcg aatacttagc
     7861 aaaaaaagaa aagaaattta aaaatatctc aaaaataaat aagacgatta gaagataagt
     7921 gtagtttttt tgttcattct atgagtctat atactattaa tgtattaaat ttgccattaa
     7981 tttgttactt taatgtgatt ttgatggcaa atttggtttg tggtattatt taaagcaatt
     8041 tattaatttt tttaataaag tttgattttt tatatgatga tatatatatg tgataatgta
     8101 tacatatttg ttacgatagt ctcctttata aagaggtctt atgtaagata agaaaaggca
     8161 tatttatact aatacataat agagtatatg cttatgtatg atagagatgt ttcttaagag
     8221 caaaactgca atattgctaa tttataaagt aaaaatatat gaattataaa atacaaataa
     8281 gatatttata aaaattattg ttttaattta taataaaaat tttttgttga aaacaatgct
     8341 cgaacctata aaactattaa ctaatatttg taaatgtata cttttgatga taaaatataa
     8401 ttaagttaag ttggttttta taaacataaa tttattttag agaggtgaaa taaatgaaaa
     8461 tatctagtca atatagaagt caatattcat ttagacatga aagtaatata aataatacaa
     8521 gaataaatga aagtatggtt aagaaaaatg aaactgtagg aaaagacact tatttatcta
     8581 atattatgaa acaaaagcaa gaacttaatg atagaattag agatttaaaa tatagacaag
     8641 aggtttatac taaaaaaata aatgaagcaa ttaagaactt atataaatca gaaataagag
     8701 aaacaactaa taacttttct aatatagaaa taggtattaa aaatagcatt atagaagata
     8761 aaaataaaag tacaatgtta gatgaaaatt caacttatct aaatacaaat gatgaaaaag
     8821 aatctttaat cactaaagag tctaatgaaa aaattgaaga agaaatatta aatgatgaaa
     8881 aattagaaga gttagaacaa aaaaaggatt ataaagagga ttctaataaa aaagagaaag
     8941 tatctgagga cttatcttta gtaggtaaaa ctcgtgaaga gcttgaaaat atgcttaaaa
     9001 attttataaa tttaacacaa gaagaaataa tgaaacttga gtcgagaata gaaaagttag
     9061 ataaagatgc tgaagaatac aaacaaaatt caaagactaa tatatttgat aaaacagatg
     9121 aacaaaaaaa acatataaat gtactgattt aaacaatatg ttttaaaaaa ataagtttaa
     9181 tttactctat ggaggaaatc caaatggagt tgagcttcga gcagatggta aatattatta
     9241 tataaataag ggtatggaag ggctaggatt ttatgcagca gcattgtcag gtagctcata
     9301 tcagagaata ggctttggaa aacttgaccc tatagaagta atagccgatg gagattggat
     9361 ttcttacaaa caagctcaag atacacttac agtaatcagg aattttttaa atagttttga
     9421 ctggagaaat gcatctgaga tggaaagagc taatagagct gcaaaattgg taaccgaagc
     9481 aaaatatgta gactctaaat attgtaacat agtctatggt aatctggttg ataaacgtgg
     9541 tgtatgtgga agttttgcaa gtagctttca tttattaaca aggttaatgg gaatggattc
     9601 actatctata ttaaatccat cattaaatca tgcttggaac tatatacaaa ttgatgggaa
     9661 atggtatagg tctgatgggt ctgaaatatc agcttttggt ggagcattag actttgatta
     9721 ccgtaaatta aaggatgcaa caagagagat gactacttac tatgatgcta aggcattgtc
     9781 tatattagga tttaatcaat aaaaataggg gaggctttat aaagagcctt tttttattga
     9841 tttgaaattt aataaggaat tttatatgaa aatttaatca ataaaagcaa aaaaggctct
     9901 ttataaagcc tttctaagtg tttttttaag taaaattttt tatatcaaca tcagttttaa
     9961 tcaaaaaata taacattttg gacttacttt taaaaataaa attattaaaa tataatatta
    10021 atcatgttaa taatgttttt agtttattat attctataat agtggtcatg ttgactattg
    10081 tagttttatt aagaatagtt ggtttatata aatatatgca aagatagctt gttgtttact
    10141 ttacttatta gtatatagga caagatactc taataaattt gcctatttta atatcttttg
    10201 tactaaaacc aactaaagtt tcctttatta tttcgttttg aataatacaa agcataatcc
    10261 gattgtttgt ataaagactg gaaagtatca gatttatttt ctaatttaga tatacctata
    10321 gaacaagaat tactctctag acataattct aaaactttct ttgctttatt ttcaacattc
    10381 tcttttgatt gattactata ttcaaaaaca gcaaattcat ctccaccaag tcttccaata
    10441 atagcatcat ttacgaatac tttagtcaaa atatttgaaa actgtattag tatttcatct
    10501 cctcgaacat gaccaaagat atcatttacc tgtttaaaat tatctaaatc aaagatgtaa
    10561 aagtatccat atgaaacagt tttaagcatt tcttttatgt gtacttcaaa aaaatgtcta
    10621 ttgtatagtc cagtcataag gtctttttct gattttagtt taaattcatt tgccattgct
    10681 agtgcttctt gtgctttttc agttattgtt ttagggtaat actcatcttt catttggtca
    10741 aagtttggaa tagaatggtg aacatggata agtttaatca tatcatctgt aactctgtat
    10801 accatagtaa atcgagtatc catttctaca tagatatctt tttctaggta atctttttct
    10861 attacccaaa gagtaccata tactataaaa gcttcgtctg taactttggt acatgaatac
    10921 cactcatcta taatctcaaa gtctatttgc tctgattctg tttggtcttt ttcaagacct
    10981 ataataaatt cttctaatgt ttcgtaaaat tcatgttttc ctgttccaat taatgcaagg
    11041 ttgttatcta ataaggaaat aataaattta aggttttctt cttctggatt taaaatataa
    11101 atatgccaaa ttttttttga taaatctctt aattgttttt gtattgtatt attttccata
    11161 gattaccccc ttaagattta cagtttatat tttactgtat tataagttga actcttatta
    11221 caatattatt ataatataga actaaagaaa ttacatcctg tataggtaaa agtatatctt
    11281 ttacaataaa aatattacag taaaataacc agttttaata tacgtattat atttttaatt
    11341 tataaacgaa tattataaat gaaatttaaa ttcaaccctg aagaaataag ctaaatgtat
    11401 tagtatataa atccatactt gaacataaat aacctttatg atatgatatc aataataata
    11461 ttatgagagg agtgccttga tgtcggagtt atataatggt aaccaacaat taaatagttg
    11521 gagacacata tattctgtat aatcagaaat tttaagtgta tttttaatac atttaatttt
    11581 tgtgtcttat tctgccttta tagataactg acatatgaca acaaatagta tttaaaatat
    11641 atattacttt gttaaattat ggtgtagtag gctttaatct gctacacttt tattaatttt
    11701 gtatagtatt attttgataa ctattttgaa atagagagca ttaatttttc tagtccacag
    11761 gagttttctg tttttgtgct gttaaatata agcacagaaa aaggcagata tatacctgtg
    11821 gacttttttt atataaatta tgaaatatgg aggtagttaa aatgagtata attgaactta
    11881 atgaactgac taaatcttat agaagatttg agaaaaaggc aggatttatg gggagtgtaa
    11941 agtctttatt taataggaaa tatacagaaa aaacagctat atcgtcattt gatttaaaga
    12001 tagaagaagg agaatttgtt ggattaatag gtcaaaatgg agctggcaaa actactctta
    12061 ttaagatgtt aactggtatt attgcaccgt cttctggaga aatatctgtt atgggttatt
    12121 accctaacaa attagaaaat aattttaaaa aacaatatgc tgttgttatg ggccaaaaaa
    12181 gtcaactatt ctttgaattg acaccagcag atacgtttct tttatttaaa gaattatatg
    12241 atatatctaa tgaagaatat aaaaaaaact tggattactt tatcgaatta tttgaggtat
    12301 ctgactattt aaatgttcaa gttaggacgc tttctttagg agaaagaatg aaaatggaat
    12361 taatagtagc ccttctacat aatccaaaaa tattattttt ggatgaacca actataggct
    12421 tagatgctat agcccaaaaa caaattaggt atttcctaaa agaagtaaat gaaacaaaag
    12481 gaactactat tatacttact tctcattaca tggaagatat taagagtctc tgtagtagaa
    12541 gtattgttgt agaaaatagc cgtaaaatat atgatggttc tacagagaaa ctatttaatc
    12601 attttcaaaa atataaaaaa ataacagtta gtttttccaa tgaaactaac attgtatttc
    12661 caaaagattg taaagttgtt gaacaaacaa atcataagac atcagtaatt gtgctaaaag
    12721 aaagaggaaa tgaaacgatt aaatggattt ttgataaata tgatgttaaa gatgtaatga
    12781 ttgaagaaga agatataggt agtattgtag agagaatata taagaaagga agtgatgagt
    12841 attatgagca actataatga agcaagttca aattattcaa tgttttataa atttaaacaa
    12901 agtattagaa ggtattatga agtagccaaa attaacttta aagtgcaact tatctggaga
    12961 tttgatgtgg tggttaatat ggtgtttacg attgtaaaaa ttttatttgc ttatatttta
    13021 tggaaagcaa tttttggtga aaataaaata gtctctggtt ttacatttaa ctctatgttg
    13081 tcttactata taataagctc atttttagca ggtattgata tgtcaaagag taccagtgaa
    13141 gagatatgta atcgtgtaaa attgggcaca ttttcaaagt acatgattat tccaattaat
    13201 gttcaaaatt attttattgc taaaaatttt ggaatgacat gtttttattt tgtatttaat
    13261 atgatttctg catttgtatg gatttttata tttaaaatag attttgcctt tacatcaaat
    13321 ccagttcaaa tattaatttc aattttgatg atttttctag ggtttatttt tatgatgaaa
    13381 ctccacttct ttattgggat tttgtcattt aaatttcttg agattagttt ttttagaatg
    13441 ataattgaaa atattgttga atttattaca ggaacaatga taccacttgt actcttacca
    13501 gaaactatag taacagttat gaaggtattt ccattttatt atacaacata tttgccatct
    13561 atgttactaa taggtagaaa cagcaatgag gcaatacaag gattaattat tctagcatta
    13621 tgggttttat ttttttctgt agtaaataaa gttatgtata aaagaatgag aacattattt
    13681 gatggggtgg gtatatgatg aaaaaaaatt tgaaaataat atttatgtta ttaaaaatga
    13741 aattatcaaa gatgatggta tttagatttg atttttttgg agcatttttt gtagatagtt
    13801 cattatttat attacaactt ctaatgttta atagtatata ttctcatgta gactcaatag
    13861 ggggatggca acaaggagag atgttaatat ttataggaac tttctcatta ataaatgcga
    13921 taaatatgac gattttcttt tttggtattt atgatattcc acgaaaaatc caagaaggag
    13981 aattagacta ctatattaca aaaccattag acccactatt taggttaaca tttgaaaata
    14041 ttaatattgg ttcatcacca ttaattctag caagtatact tattgtttta tatggaatat
    14101 ctaagttaaa ctcagagata actttagtta tgataataat atatattttc atggtgttat
    14161 tgatgacatt acttttttat gatgtatgta tcatacttag aacagtttca ttttttgtta
    14221 tatcatcttc tgcgataatg agattagaag ataatttatt gccaatgaat atgaaaattc
    14281 ctggtgtaat atatgaaggt gtttttaaag tattattcta tttaatctta ccatatggaa
    14341 ttatgtcgac aattccgact caaattttgg ctgggacaat aacaattaat ggattaatat
    14401 actcaatttt aattgtattt ttgtttacat tgtttatgtg gagattttgg aagttaggta
    14461 tgagacatta taaaagtgca agtagttaat aaattagtaa aaatagacaa tgtataacta
    14521 tatataaata cgtttcatat gacactaaac tatgtgaaca agtatttgcg ctaaaggcac
    14581 tgaaagctat aaaatgatta aagtaaggat ttaaaagttt ctttagtaga aatataattg
    14641 tgtaaaaaag caagattaat taaaactata tccaaataat tagatataat tcttatgcag
    14701 aaaggaggtc tgtaaatgtc taattgtttg gaatatattt taaaaataat taatgtacag
    14761 aaaaataaac aaaatgagtg atgtatatta ttcagaaaat catgaacaag ataatcttta
    14821 tgagcaagat aatttactgg cacaaagatt taatttattt actctattga agtttgcatt
    14881 tcctactatt tttatgatga tttttatggg gttgtataca atcgtagata ctatatttgt
    14941 atcaagattt attaatacaa atgctctatc agcaattaat attgtatgtc caattattaa
    15001 tcttatagtt ggaattggaa ctatgcttgc cactggagga agtgctattg tagcacgtaa
    15061 gatgggaaat ggtgatattg agggagcaaa taaagctttt tctttaatcg tattatcagg
    15121 agttgtaatt ggggtaataa ttactggtat aggtattgta tttatggatt atatcattat
    15181 atggttagga gcaagtgaag ttttaattaa atattgtcgt tcatatcttt ttattatctt
    15241 ggtttttgct tctgcaaata tactacaagt attatttcaa tgtttatttg taacagctgg
    15301 aaagcctcaa tttggattat ggctagtgtt aggtgctggg tgtgctaata taattcttga
    15361 ctatgtgttc attgttctgt tacaaatggg tattagtgga gcagcacttg caactggagt
    15421 aggataccta attcctaccg tttcaggtat ggttttcttt tcaaaaaata aagagttact
    15481 taagttttgt atacccagac tagataaaag aatattattt gaagcttgtt ttaatggttc
    15541 atctgaaatg gtaggtcaac tatcaacagc agttactact tttttattta atgctatgat
    15601 gatgaaatta attggtgagg atggagttgc agctattaca ataattattt attctcaatt
    15661 tcttttaact tcgctttata taggtttctc aatgggtgtt gcaccaataa taagttataa
    15721 ctatggaagt gaaaataata cacagttaaa tcatgttttt aagatttgta tattatttat
    15781 ttcagtggca tctttattgg tatttatctt ttctatatca tgttcaccaa tcattgtaga
    15841 gattttttct caaagaggaa gccgagttta tgaaattgca aggtctggat tttatatatt
    15901 ttcttttagt tttatttttt gtgggtataa tatttttact tcgtctatgt ttacagcatt
    15961 gtcaaatggc aaggtctctg caatattatc ttttctacgt acatttggat ttataactat
    16021 aggtttattg atactgccaa atatattaaa tataaatggg atatggttag cagtaccaag
    16081 tgctgagatt ttaactttat ttttaaccat tttttgtata tgtaagtata agaatagata
    16141 taaatatatg tagatattaa ataaatagtt taggtagata ttttatgttt taataagtaa
    16201 ttcaattagt ggtagatata tactattgaa aattaaagtt ataattgata taaagaatta
    16261 ataaaaataa catataaaag tttaaaaggg agttgaactt aaagtttgat tagaaaaagt
    16321 caattttaag acaactcctt ttattttact attgtattaa tctgtatctg tatctgtaag
    16381 tgtattagtc taatattata aactttggat gaaattaatt ttctataggt tttaatgtat
    16441 catacacttt attttaaaga aacttatctt aattttcata gtgattgaat ttgtctatta
    16501 gagatgttac tattagtaat gtaaaagcaa gagtagtgaa tataaaaaaa caagatattg
    16561 atactgcaag attagaatat aataaatttt tatggagaga tttcaatatg gatacatatt
    16621 ttaataaaaa aatattactt gttgatgatg aaaaagatat agtagattta attgaagaag
    16681 tactcataaa tgatggtttt aaaaacatta ttaaagctta taatggttta gatgctattt
    16741 cgctgtgtaa gatagcatgt ccagatgttg tcattcttga tataatgtta ccagatatag
    16801 atggtataga agtgtgtaaa aaaattcgag agttttcata ctgttctatt ctttttttat
    16861 catcaaaaaa tgatgatatt gacaaaatac taggtcttag tagtggggga gatgactata
    16921 taacaaagcc attcagtcca agagaaattg cttttcgagt taaagcgcaa ttacgtagac
    16981 aacagtatca aagcattgta ccatcagata gtgcagttat taaaatagga gatattacta
    17041 ttgatataga aggaaaccgt gtatataaag atagaaatga gattgagtta actggaaggg
    17101 agtatcatct tttaagctac atggctcaaa atgtaaataa aattatagga aaagaaagac
    17161 tctatgaaca agtatgggga gtatatagta gtatttgtga caatacaatt atggtgcata
    17221 tacgacatat aagagaaaaa attgaagata acccctcaaa tcctaaaata cttataacgg
    17281 ttaaaggact aggatataaa cttgtgaata gaatcgatta gaatagattt taatattaat
    17341 gaagatgtct caagttgaat ttttagttaa tgaggcattc ttttatgtgt gaagtcaaat
    17401 gtattttcgt tatctcaaca atgaagaaga ggtctatctc tattttgaga tagcctcatt
    17461 aagtttatat cctggtaatg tactttatgt tctaataaaa tttttttagt ttatttgcag
    17521 tgaaacatta actagaacat taaaatttgc attgaaatat caattaaata atattgtata
    17581 ttgtgtataa acttatacta attgtttttc tatattaaac actttatcaa tccaaccttt
    17641 ataaaatttt tcttcatggg aaactaggat tatacttcct ctaaactgtt tcaaagcatt
    17701 ttgtaaagca tcctttgtct ctgcatcaaa gtgattggtt ggttcatcca gaataagaaa
    17761 attacaagga gataacatca tacaacataa tttaacctta gattgttcac caccacttaa
    17821 agtagataca ctccttgaaa catgctcttc cttcacacca catctggcta gatgacgtct
    17881 aatctctttt gtatttaatt ttggatattt atctgctact atttgaagtg gggttttatc
    17941 aggattttcc cattttaaat cttgctcata ataaccaatt ttaacttgtt ctgaaaactg
    18001 gaaatctcca gatatacgag gaatatcttt gactaatgtc tttaacaagg tagatttacc
    18061 gattccatta aaaccagtta taactatttt ttgacctcca tcaacagaaa aatttaattt
    18121 tggaagtaaa gaatagtagt atccaacttc aagatttgtt atagtaagag cattttgagc
    18181 cgatatttca atttctgaaa attgaatatt tggttttcct gtaaaactag gaggagctat
    18241 tctttctatt ttatccaatt gtttttgtct tcctcttgca atttttgaat ttactccagc
    18301 tttattttta cgtataaatg tctcttcttt ttctattttc ttttgttgtg cttgatacct
    18361 tctaatataa tcttctctta aatgtgattt ttgtttaagg aactctgaat attttccatg
    18421 atatttttta atcataccaa attctatatc aagaatacct gtagaaattt tttctaaaaa
    18481 atcaaaatcg tgggaaacta caataaatgc cccattaaat gtatttaaat aatttgagag
    18541 ccattcaaca tgttctttat ctaaaaagtt tgttggttca tctaataata gaacatcatg
    18601 gttagaaagt agcaattttg ctaaaattac ctttgccctt tgcccaccac taagctcttc
    18661 aacaacacga ttcattccta ttgaatctaa tccaagacca tttgctattt tatttatttc
    18721 attatctata gaatagaaat tacttgctat tagttgttcc tgataatctg atgctttgat
    18781 tagatattgc tcattttcag atatagcact tttttgatag agtaaattca tttccttttc
    18841 tattttataa agttcttcaa atgcagtgtg taaatagagt gatattgttg tatctctatt
    18901 gatttctgca tattggtcta aatggcctat attgatatta ggttgccact tgatttctcc
    18961 tgaatctgga acaacttctc ccaataaaat tttaattaat gtacttttac ctgtaccatt
    19021 ttggccaact acacctatat gttcaccttt gaataaatca aagttagctt tctcatacag
    19081 tgatttatcc ataaaacatt gtgataaatt agttactttt aataaactca taattcaaac
    19141 tccttttctt ttattaaaaa atttatggaa ataaaaaaag cagaggaatg gcattgtaaa
    19201 atgccgacct ctgcttttgg aaaaacaaaa ggtcatagac aagcattgtt gtctatgacc
    19261 agaaaatcta tactatctat cggcaaatag gtaattttaa ataagcatag ccaaaaaact
    19321 atactaaacc tatatttgcc gatgaagata gaaagcttta agctttcaga ttaacctcta
    19381 cacaacgctt catcgtatac atttgtgtag agttaagttt tctaacaagt ttaactaatt
    19441 ctatataaat cataattatt cacatcctaa ttttagtatt aattctagtt tatctatatt
    19501 taaattgtac ttttgtactt tgtggattat cttatttatt taaaatatgt tttattaatt
    19561 atagcttaag ttatgttatc ataatacttg gttatagtca ataagaaagt tatatttgct
    19621 atatattata ttaatttagt aaatctatca ttacaaagtt atttgaagac tcgctaccaa
    19681 taaaatttta tagtctatgg gaaaattaag attatgagtt ttatactttt gatataggga
    19741 tactattttc ataaatatta caacatgtag aagtatatgt ctagtattgt tgaattttca
    19801 atgatatatt aaggttctaa tatattatac aaaataaaaa cattaattc
//
LOCUS       DAEPOG010000063        19713 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEPOG010000063 DAEPOG010000000
VERSION     DAEPOG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.063"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..574
                     /locus_tag="KR432_001862"
     CDS             <1..574
                     /locus_tag="KR432_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640400.1"
                     /translation="EDEANEFLLTNKASINKLENLINSLVNISRLEISMINIKKEDND
                     IKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESIFNVLDNAVKYTQVN
                     GEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADEIESVEGSGVGLYLS
                     RKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            691..1374
                     /locus_tag="KR432_001863"
     CDS             691..1374
                     /locus_tag="KR432_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640401.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            1367..3754
                     /locus_tag="KR432_001864"
     CDS             1367..3754
                     /locus_tag="KR432_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0640402.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            4033..5175
                     /locus_tag="KR432_001865"
     CDS             4033..5175
                     /locus_tag="KR432_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0640403.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            5194..5718
                     /locus_tag="KR432_001866"
     CDS             5194..5718
                     /locus_tag="KR432_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0640404.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            5818..6636
                     /locus_tag="KR432_001867"
     CDS             5818..6636
                     /locus_tag="KR432_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0640405.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            6714..7025
                     /locus_tag="KR432_001868"
     CDS             6714..7025
                     /locus_tag="KR432_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH0640406.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(7328..9331)
                     /locus_tag="KR432_001869"
     CDS             complement(7328..9331)
                     /locus_tag="KR432_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0640407.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(9484..10122)
                     /gene="recX"
                     /locus_tag="KR432_001870"
     CDS             complement(9484..10122)
                     /gene="recX"
                     /locus_tag="KR432_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH0640408.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            10398..11087
                     /locus_tag="KR432_001871"
     CDS             10398..11087
                     /locus_tag="KR432_001871"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH0640409.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            11088..11660
                     /gene="yqeK"
                     /locus_tag="KR432_001872"
     CDS             11088..11660
                     /gene="yqeK"
                     /locus_tag="KR432_001872"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBH0640410.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            11777..12127
                     /gene="rsfS"
                     /locus_tag="KR432_001873"
     CDS             11777..12127
                     /gene="rsfS"
                     /locus_tag="KR432_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH0640411.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            12481..14901
                     /locus_tag="KR432_001874"
     CDS             12481..14901
                     /locus_tag="KR432_001874"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH0640412.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            15328..15640
                     /locus_tag="KR432_001875"
                     /pseudo
     CDS             15328..15640
                     /locus_tag="KR432_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15739..16407
                     /locus_tag="KR432_001876"
     CDS             15739..16407
                     /locus_tag="KR432_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0640413.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(16565..17560)
                     /locus_tag="KR432_001877"
     CDS             complement(16565..17560)
                     /locus_tag="KR432_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH0640414.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(17681..19309)
                     /gene="aspD"
                     /locus_tag="KR432_001878"
     CDS             complement(17681..19309)
                     /gene="aspD"
                     /locus_tag="KR432_001878"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBH0640415.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT         7445 a         2401 c         3191 g         6676 t
ORIGIN      
        1 agaagatgaa gccaacgagt tcttattaac caataaggct tctataaata aattagaaaa
       61 tttaataaat tctttagtga acatatcaag gttagaaatt agcatgataa acataaaaaa
      121 agaagataat gatataaaaa atactattct aaatgcagta aaaagtgtta cccctaaggc
      181 taagagcaaa agtataacat taaatttaaa tgaatttaac agtagaatta taccacatga
      241 taaaaagtgg actgaggaga gtatttttaa tgtattagat aatgctgtaa agtacacaca
      301 ggtaaatgga gaaataaata tatctgtaga agaaactgct aatttcttta aaattataat
      361 agaggataat ggcataggga ttgaaaaaaa tgagtttaat aatatattta aacgttttta
      421 tagaggttct gctgatgaaa ttgaatctgt agaaggtagt ggggtaggat tgtatttaag
      481 tagaaagatt ttagaagaac aaggtggaaa tattatagtt tcttcaaaag tagggatagg
      541 gagtaagttt tctttgtttc ttacaactat gtaatattaa atgaaaggaa aatgtaagcc
      601 tctacattta ttatataagt ataaacagct ccagaaataa aaaattaata aaatatgggg
      661 taatataact tagaatgagg ggataatttt atgaaaatac tatatactga aaatttatca
      721 aagcattatg gaaaaggaga aaacttagta agagctttgg acaatgttaa cttagaaata
      781 aatgaaggtg aatttgttgc aataatagga aaatcaggtt ctggaaaaag tacactttta
      841 catatgattg gaggtcttga tatacctaca tctgggaagg tgtatataga taataaaaat
      901 atatttacat taaaagaaga agaactagct gtgtttagaa gaagaaaaat agggtttata
      961 tttcaaagct ataatttaat tccatctctt aatgtatggg aaaatgtagt gttgccaatc
     1021 ggtctagatg gtagagaagt agatgaatct tttataaaag aacttttgaa atcattagga
     1081 cttgaaaata aacatgacgt tttaccaaat acattatcag gtggtcaaca acaaagggtg
     1141 gcaatagcaa gagcattagc tacaagacca gctataatac ttgctgatga accaactgga
     1201 aacctagatt ctaaaacatc tgatgaggtt atgagtatat taaaatctat gtctaagaag
     1261 tacagtcaaa ctctagttat gataactcat gatgacagta tagcccaaat ggctgataga
     1321 gttatattca tagaagatgg cagggtgtca aaggttggtg ataaaaatga ttaatactac
     1381 taaaatagca aaaagtaatt taaaacaaaa taaatcaaaa agtattctaa taataatcac
     1441 tataatactt tctactttaa tgctttcttc tattggaatt tatattgttg atgcaggagc
     1501 atatcaaaaa gaaaatacta taaagtattc aggtaattat caaggtatat tagcaaatgt
     1561 agatgaaaaa caggctgata tattgagtaa tcatgcagat gttgaattaa ctggtgaaat
     1621 gaatggagta ggtgttgaaa aactggaaga tgattcaaat atttctctag catacatgaa
     1681 tgaagatgct ttgaaattaa atagttttga acttatcaaa ggaaaattgc caacaaaaga
     1741 gaatgaaatt gtgttagata gtggagcttt aaaggcactt ggttataaaa ataaagactt
     1801 aggtgaaaag ataaaaatat cttataatga ctataaaaat gataaaaaaa ttgaaaaaga
     1861 gttcgttata agtggaatct taaaaactag cgaaatatct gaagcaggta aatattatta
     1921 tgctattata tcagaattat atatgagaaa tactagagat atgtcacaag aagattttaa
     1981 tatttatgta aaatttaatg ataaaagtaa tttatcaata gagcaagcaa aagaaaaatt
     2041 agataaaatt gctaatgata taggacttga tactattaat actgctgtaa atgaaaatta
     2101 tataaatgct ttaaaaccag atatggaaac gattatgggt ggaggttttg taggattagt
     2161 aattgtatta tctagtattt tagtaatata caatatattt tatatttcaa tagttacaaa
     2221 agttcaagaa tttggaaaac ttagagcaat tggtgcaact aagaaacaaa taaaaaatat
     2281 agtgtttaag gaagggttta tattagccgg aatagcaata ccaataggaa taattttagg
     2341 ctatgtactt gcaaatatta taataaaatc atttatggat atagatgtaa aatcatcacg
     2401 attaccagtt atattatcgg ttgtggtaat aagctttata tctgttgttt tatcactttt
     2461 aaaaccaatg aaagtagcat caaaagtatc cattgttgac gcagtaagat atacaggaaa
     2521 taaaataagt aataagagta agagaaaagg atataaaaat ataaatctaa agagattatc
     2581 acatgccaat ttagaaagaa ataaaaaacg tacttacatg acattggcat cacttatact
     2641 tagtggaact atatttataa cagtatcaac tgctttagaa agttttgatg ctgagaaaat
     2701 ggcaagagaa cactttcctt atgatataga agttagacta tctggttatg aaatgaacag
     2761 tgataaaaat cctaaaaata atttgaatat tttacaaatg gataatcctc taagtaaaga
     2821 tttttttaat caaataaaga acatggaagg aattaaaaga atagaatctg caagaagtgt
     2881 aaaaattggt atggaggact atgatgttga atttaagtat gaccttttac aaagtataaa
     2941 tgaaaatgat gtaaaatcat taaacaagaa tctaatagat ggaaaaataa atcttgaaag
     3001 actgcaaact ggagatgaaa tagtaattac tcatgttgat acagcaaaag aaatgggagt
     3061 aaaagcagga gataaaataa gattaacttt atatgatgga gataaaaaga taaaaaaaga
     3121 atttaaagtt caagcaatag ctatgggggt tccaagtttt ggtataggga aggattttat
     3181 tgatagaact ttaaaatacg attcaactag tagcttggga atatatacag aagaaggaaa
     3241 gtatcaagaa atcaaagata gtatcaagaa gattgctaaa aataatggat ttttagagac
     3301 tgattttata gatagtagaa ttgagtcaaa taaggctgtt atttcattta taaagataat
     3361 gggatacacg ctaacaggca taataggtgt gattggattt atgaatcttg tcaacacaat
     3421 gataactagc atagttacta gaaaaaaaga acttggtatg cttcaagcta ttggtcttac
     3481 taataaacaa ttagtgaaaa tgcttaattc agaggcaatg tattacactt ctggaatgat
     3541 gataggaagt attttatttg gaggtatatt agggtatatt gctgttatat ttctaaaaaa
     3601 gactggatta tcttatgcaa catattcttt gccaatagta ccaatcttgt tgatgattgt
     3661 gtgtattctt atagctcaat ttataacaac ttatttgata ggacgaagtt ttaataaaga
     3721 atctcttatt gatagagtaa gatacagtga ataaaatgca aatatttgtg gttagtttaa
     3781 ctagcaaaaa gcatataaaa aatatagtta aagagattca taattaaata ggttaaagca
     3841 tagtacagag caaacaattt aaacggatgt ttgctctgta ttttattatg taatcaataa
     3901 tatatctaat atactgatat tattcatgtt agaaaatgta tcggtacaat tctaaaaaag
     3961 tataaaattg taccatataa aatgaaaatt ttgtataata atcgtataga aaataaatag
     4021 gagctgatat atatgaataa gaagattaat tatgcaccag gaccaactga aactagggaa
     4081 aatgtaagat tagcaagagc agaaaaaaca acaaatccag atatagatat tgattttgtt
     4141 gaattttata aaaagacatg cgagaagttt ggcaatatag ttggaacaaa aaacgatgtt
     4201 tacatattga gtggtgaagg aatattaggg cttgaagcag catgtgcatc acttaccgaa
     4261 aaaggagata gagtacttgt aattgacaat ggtatatatg gagaaggttt taaagatttt
     4321 gtaacaatgt atggtgggga gtatgtgctt ttttcaagtg aatatactaa aagtatagat
     4381 attgatgaat tgaaagcatt tttagatgag gatagtaatt ttaaatatgc aacagtagtg
     4441 cattgtgata ctccaactgg cgtattaaat gatgtaagca aaatatgtcc attacttaaa
     4501 gaatatggaa tattaactgt agttgattct gtagcaggta tggtaggaga aagattaagt
     4561 gtggatgaat ctaaaataga tataatatta ggtggttcac aaaaagctat ttctgcacca
     4621 gcaggactta ctatagttgg aataagtcaa gatgcaaaaa attgtataaa aaatagaaaa
     4681 actgatgtaa taggttttta ttgtaattta aatatatggg aaggctatta tgaaaaaaaa
     4741 tacttccctt atacaatgcc aataagtgac ataatggggc ttgataaagc acttgataat
     4801 atattagaag aaggaataga aaatgtatta gatagacatg aaaagatagc ttatagtgtt
     4861 agaaaatcta ttgaggaata tggtctagaa ctatttttag aagaaggata ttctaataca
     4921 gtcacagcta taaaaattcc tgaaagtata ggagctttaa aacttactga ttatatgctt
     4981 aaaaattata atacacttgt tgcaacatca ctaaatcaat atatggatac tattttaaga
     5041 ataggtcata tgggagagaa tgcaaatcta aataaaatag agcatgtact aaatgtcttg
     5101 gataaaagtt taagtgcatt aggattcaaa ggaaatgaaa atttgttgaa tttatttaat
     5161 aagtattatt tttaatagat agggggatat agtatggaag ttacagctaa gcaaaaattt
     5221 acaacgaaag acatggtgga aacctcacta ttaatagcat tagtatttat tgcaactaaa
     5281 tttataaata taagattacc aatatctata aatggtgggc ttgtgcattt aggaacagca
     5341 atgttgttca tatcagcaat agtatttgga agtaaaaaag gtgctttatc aggagctatt
     5401 ggtatgtcac tttttgattt aatatctggg tggactttat gggcaccgtt tacctttata
     5461 gttagaggag ttatgggtta tttacttgga aagattgcat gggcaaatgg taataatggg
     5521 aataattttt taatcaatgt aataggaata tgtgtctcat caatttggat gttatttgga
     5581 tattatataa cagaagttat actttatggt aattttatag tacctcttac ttcaatacca
     5641 ggtaacttaa tgcaagtatt gataggtctt ataatagcgc ttcctatttc taaggtattg
     5701 aaaaaatgta taaaataaat ataaagtgaa ctaaaattta tgatagcata aattatactg
     5761 tataatatta agatggagta cataattaaa aaattttagt atagaggtta gttcacaatg
     5821 ttatcagtta caagatattc aatcaaaaat gttcgtttaa agccgtgggt aaagtttatt
     5881 tggtattttg aaacaaaatc aaatatactg ttaaataata agttacttcc tacagacagt
     5941 atagatatta ttcttaatct ttctgatgtt atggaatata aaattgaaaa tcaagactat
     6001 actgctagta atatgcattt taatggaata agggataaac atggatttat tattcaacat
     6061 ggaaatatac gtgttatagg gatttctttt tatccatttg gtctctatcc atttttaaag
     6121 ataccaattt cagagtttaa cagacaaatt gttgacttag aagcagtatc agaactattt
     6181 gccaaaaaat tggaagagtc attaaatcca acacaatcag tagaaaaaat tgttttatat
     6241 ttagaagaaa tgttattatc catattagaa gaagatttaa tttcaaataa atctgtaaag
     6301 cttctaaatt catttatata tgataataga tatagcaata taaaagcatt ttgtgataat
     6361 acaaatataa atattaaaac attggaaaga atctgtttaa aatatacagg gtatacacct
     6421 aagattttaa agagaattta cagatttaaa atggcaagta gtcaacttat ttataattat
     6481 aaagataatg aactttttga ttttatttat gaaaatgagt actatgacca agcacacttt
     6541 ataaaagaat ttaaaaaatt ttcagggacg tcaccaatta aatttattgg tgagaataag
     6601 acgattaaag aaaatataaa gtacaactat ttataattat actgtcgatt ttttacaata
     6661 cctccaattt attttttgat atgatatagg aaaataaatt ggaggtttta catatgaaaa
     6721 ttattacaga aatagttgag tttaatgtgg aagaaaattt atcaaaagaa gagtttatag
     6781 aaattgtaaa tgacttagaa ttaaaatttc attctattca atctggtttt attgatactg
     6841 agcttttata tgatgaaaag tctcaaaagt ggattatgat acaacattgg gaatctatag
     6901 aagacctcaa gagtgcttct agtaaaatgt ttaaagattc ttcaactgag aaatttagaa
     6961 aagctttaaa gaatcaaact gttaagatga atataatacc acaaattaaa agttggaaat
     7021 tatagaactg aatgtaaaag atagttaaat agtatcaaac taaattagcc aaataacatt
     7081 ttgtatttaa attgttattt ggcttttagt gtttacttta atatgagtgt tatatactag
     7141 aaaaaccaac aaactgttag agtgtggatt ataataatgt catatttggg agctaaaagg
     7201 cttaatactg atttgtcaat atgtcttttt agcatgttga acaggtatat tgccatgctt
     7261 tcattatttt atttttttgc cttatactgg actacatttg cagtttccta atatatttgg
     7321 ctcatattta aatgccatta tctaaaagta ctattgctgt acgagaggca ataggcattt
     7381 tagaagaagg aggagttgag ctaaagtata catcataatc tcttaagttt tctttttttg
     7441 tgtttcctcc aaactctacc atatcagtat ttaatatcgt tttgtagttt gtattttgaa
     7501 actttgaaat atcaactgat tgtattagat tagggtggaa attaaatata aataaatatt
     7561 tgcccttact gtacaccaaa atctttttat cattatccat atgtataagt tcatgactat
     7621 tttggataaa aatatcactg tattttgtaa gttcgagcat agatttatca aaatttaata
     7681 attgtttata ctttaagttg ttatcttctg aaaggctcca ttgtcttctg gcatacttat
     7741 agctccaatt gttgccttct cgtggaaaat caatccactc aggatgtcca aattcatttc
     7801 ccataaaatt tagatatcct tcacctgcta gagaaaaagt tattaattta attaatttaa
     7861 taagagatat tgctctattg ataacatggt tatttgagtt tatttccata ttccagtaca
     7921 tttccttgtc agctagccaa aaaataattg ttttatcgcc aactaatgct tggtcatggg
     7981 attcacaata tcctatattt ttttctccag gtcgtctagt agtaagttca taccacattt
     8041 ttcccaaatc ccagtcttca tcagataagt tagagatagt ttttatccaa aaatcaggta
     8101 ctcccattgc gagtctatag tcaaatccaa ttccgccgtc ttttataggg atacacatac
     8161 caggcattcc actcatatct tctgcaatac ttatcgaatt aggtttaatt tcttttatta
     8221 attcatttgc aaattggaga tatgttatag cttcaatatc tgtattcata ctaaaatatt
     8281 tttcataact atcaaatgaa actccaagac catggttatg atataacatt gaagtcacac
     8341 catcaaacct aaagccatca aaatggtact catttaacca aaattttata tttgaaagca
     8401 aaaaatgtat tacttcaggt ttaccatagt taaaaagttt tgtaccccaa gcaggatgat
     8461 ttccctttga acctgagtgg aaaaattgat gttcacttcc atcaaactca tttattcctt
     8521 ctaaagtatt ctttacagca tgagaatgaa ctaaatccaa taaaacagca atccccattg
     8581 aatgagcagt gtttatgagg ttttttaagt cttcaggagt accaaaccta gatgagattg
     8641 cataaaagtt tgaaacttgg tatccaaaag atgcataata tggatgttcc ataatagcca
     8701 taagctgaat ggtattgtat ccagcttttt ttatcttagg aagtattttt tcagtaaatt
     8761 cattataagt tccaatagat tcgctttcag tagacatacc tatatgacat tcatagatta
     8821 gtggagaagt tatgtttttt aagtcaaaat tattgtcagt ccagataaat ggagtttttg
     8881 gttgccatat ttgtccatta aatccaccaa aatctttttg aattactctt tttatatata
     8941 atggtatacg gtcaaatgtt tttccatttg cagtaacttg tacctttacc tctgatttgt
     9001 gaggtaagct attttgacca ggaataaata tttcccaatt accagaccca atttttctta
     9061 gaggatgaga ttttctattc caattattaa agtcaccaat tagtgacaaa ctatctgcat
     9121 ttggagccca ttccctatag aagaatccat catttgtctg atgaaatcca tagtaaagat
     9181 gagcatttgc aaaagaacta aaatctttat agtttttcaa tgtttttgat ttcatatcct
     9241 cataatgttt cattcgcttt tttatatcag attcaaatgg ttgtaggtat acatcaatat
     9301 ctaaaatttt aaatcttttt tgacttttca ttgtatttct cctccataat aaatattaca
     9361 tttaaaaaat aatatttatc tcatactatc ttctctgtat aattgatata tattatataa
     9421 tcctaataaa aagcttaagc caccaatgat taatttcata atttacctcc ctagtattta
     9481 tacttatata tcgtattcat caaaatttaa aagtttgttt aaaactcttt taataatatc
     9541 ataatcaaag cctttatagg aaagatgttg agaaattttt tgatatattt tctttttatc
     9601 ttctttcttt actctttcat accttttttt tgctagatac atagcatttt caaattcagt
     9661 gtccttgtct aattcagata ctgcttcatt gattgtactt ctattgatac ctttattata
     9721 taaattctgc ttaattctgt ttttgccaca tttatttagg ttaacatttg tgttaacaat
     9781 tttttgagct aatacactgt catttacgag attataactt ttaagaaaat ctatgaccct
     9841 ctcaattata ttttcatcaa attctgatga tagtttttct tttaattttt tttcagattg
     9901 gtcagcatga gataaaatat ttaatgcttt atttttagct tttatataca tttcatcatc
     9961 taaaataggt tttaattctt cttcatttat ttcattccct ttttttaaat taaatgtata
    10021 aacaagttct ttaaatatag caataaaaaa tttatcatct acataaatat taactctatc
    10081 atcatttctt ttttgttgtt ctatttttgt aattataccc ataagtcctc cttttttata
    10141 tgtaattata ttaacaaaag caactaataa aatttaacaa aaaacttata tttgtatgtt
    10201 atattttatc aaaattaaga caaaatagta aaaagttatt tttattaaaa agtaaagcag
    10261 aatcatgtta aataattatt ttttaaagtg ttttttagca tataaatata attatatacc
    10321 caaaaaaaca aattataagc tttaagaaat aaaaaataat ggtacaatag tattataaca
    10381 ttaaaaggag acaaaatatg agaagtgaaa atctagttaa gatggcagag ttgaaaaata
    10441 ctgaaaaact agagaaattt aatcgccata aaggcaaaat caaaataggt atattgggag
    10501 gtacttttga ccctatacac tatgctcatt tagcaacagc agaatttatt agagacaagt
    10561 atgacattga caaaataatc tttataccat caggaaatcc accacataaa ttatgtatca
    10621 ctacagataa gtatgataga tataatatga cacttctagc aactgaaagc aacgaagatt
    10681 ttttagtttc aaaagtagaa atcgaaagaa agaaaaggac ttatacaata gatacattaa
    10741 aatacttaaa gaaaaaatac aaaaatgcag acatttattt tataacaggt gcagatgcaa
    10801 tttgtagtgt tgaagagtgg aaagatgtaa agaaaaactt tgagttagct acttttatag
    10861 ccgcaactag accaggaatt agtttgttaa ggtcacaaga gactatagaa aaactaacta
    10921 agaagtataa tgctgacatt ataacagttt atgttccatc actggatata tcgtccacat
    10981 atataaggga acagctaaat gaaggcaagt caatacgtta cttagtaccc gaaaatgtag
    11041 aaaattattt atatgaaaat aaactatatc aatatggaga tgattaaatg aacttagaaa
    11101 atattaacca aagactaaat caaatgctac cagtggggag gcttagccat tcaagaaatg
    11161 ttgcaaaatg tgctgaaaaa ttatgtgaga tttatggatg tgacaaagaa aaagcttact
    11221 tagcaggaat gattcatgat tgtgcaaagt atttaagtga caaggaaata gaggattatg
    11281 taaataagta tgaaatatat ttagacccaa tagaggatgg aaatcgttcc ttgtctcata
    11341 gtgtaattgg ggcgtacata tgcgagtatg aatttgaagt agaagatgaa gatattataa
    11401 atgcaatcaa atatcataca acagggagag aggacatgtc tcttttagaa aaaataattt
    11461 acatagctga tttaattgaa gaaggaagaa aatttccagt tgtagataca ttaagagaat
    11521 tagcatatgg agggaagctt gatgaagcac ttttaacttc ttttaataat actttaatgt
    11581 ttgtcatcaa taaaaaagaa gagatacatc caagaacagt tatggcaaga aactatctaa
    11641 ttaaagaaaa gttattataa aaaagctata taaaggcaaa catattaata aatttaaatt
    11701 tgactatgtt acaaaatcgt attaatatgt tacaataact aagtgtattt taattaggaa
    11761 agtgaggaat ttttatatga cagtggaaca aatgacaaaa atagcatatg atgccataga
    11821 agataaactg ggtcaagata cagttataat aaatatagga aaagtttcta gtttgtgtga
    11881 ttattttatt attactacag catcttctca gagacaggta aaagctattg ctgacaatgt
    11941 ggaagatgaa ttagcaaaac taggattaga gccaagaggt aaagaaggac aaggaactca
    12001 aacttgggta ctccttgatt atggagacat aatggttcat gtatttaatg aggaaaatag
    12061 agggttttat aatttagaaa aattatggaa agatgcacct tatatagata ttgacacatt
    12121 agcataaaca tgataatata tgttattata attagaaaat ttcaaaaatt atattaaatt
    12181 gaatagacta gagcagtaaa agattatatt ttttcagaga gttagtggaa ggtgagaact
    12241 aatattatat aactttgaac ttactagatt aaaatctatt ctccatggct ggattaaaag
    12301 ctatctaaga gagtctatat atgtagtagg ctaattaggg tggtaccgcg aagatttatc
    12361 ctcgtcccta aacgtaagtt tagtgacgag gattttttat tttcaaagaa ttatttggaa
    12421 attataagta aataatacta aataaggtag ttatacacaa ttaaaaatag gaggcaaaat
    12481 atgagcgtat ataattttaa agaagtagaa tcgaaatggc aaaaaatttg gaaagataat
    12541 aatcagtata aaatggatac tgcacaaact gaaaaaccta attattacac attagaaatg
    12601 ttcccttatc catctggaaa aatacacatg ggacatgtta gaaattattc tataggtgat
    12661 gttgtcgcaa gattcaaaaa aatggaagga tataatgtac ttcatccaat gggatgggat
    12721 tcttttggat taccagctga aaatgcagct ataaaacatg gaatacatcc gcataaatgg
    12781 acaatggaaa atatagaaga aatgaaagag caattaaatt tattaggtat tagttatgat
    12841 tgggataaag aagttgcaac ttcaactcca gaatactata gatttacaca agaaatattt
    12901 ttgaagtttt tagagcatgg gcttgcatat aagaaaaaat catatgttaa ctggtgtcct
    12961 tcttgtgaga ctgttcttgc aaatgagcaa gttgttcaag gagcatgtga gagatgtaaa
    13021 gcaacagtat taaagaaaga tttagaacaa tggtatttta agactactga atttgcagaa
    13081 gaattactta atgacttaga tacattagat ggatggccag aaaaagttaa aacaatgcaa
    13141 aaaaattgga taggtaaaag tacaggagca gatttagttt ttgatataga tggaactgat
    13201 aaatctatga cagtatttac aactagacca gacacaactt atggagttac ttatatggta
    13261 cttgctccag agcatgaatt agttaaagaa ttggttgcag ggactgaata tgaagcagat
    13321 gttgaagctt ttgttcaaaa aatgcatact atgacagaaa tagaaagaac agcagctgat
    13381 gtagaaaaag aaggaatgtt tataggtaga tatgttataa atcctttaaa tggcaagaaa
    13441 gttcctctat ggatagctaa ctatgtatta gttgagtatg gaacgggtgc tataatggca
    13501 gttccagcac atgatgaaag agatagagat tttgctgaaa aatataattt agacataatt
    13561 gatgtaataa cagaagataa caaaatgata aattcagaag aatttaatgg attagatgct
    13621 tcagaaggat ttgaaggaat aatcgataag ttagaaaaag aaggtagagg aaagagaaca
    13681 attaattata gacttagaga ctggttagtt tcaagacaaa gatactgggg ttgtcctata
    13741 cctgtggttt attgtgatga atgtggaata gttccagtta aaaaggaaga tttaccagtt
    13801 cttttaccaa cagatgttga atttactgga aagggagaat ctccacttac gacttcaaag
    13861 caatttatgt caacaacttg tcctcactgt ggaaaaccag ctagaaggga agttgatact
    13921 atggatacat tcgttgattc ttcttggtat ttcttaagat atgtggatag caataatgaa
    13981 aatgaaccat ttagtaagga attagttaat agatggcatc cagtagacca atatataggt
    14041 ggagtagaac atgctataat gcatttgctt tatgcaagat ggtttgtaaa ggcatttaag
    14101 agtatgggaa tggtagactt caatgagcca tttaaaaact tacttactca aggaatggta
    14161 cttatggatg gttctaaaat gagtaagtct aaagggaata cagtatctcc tatggatata
    14221 atagatgagt atggagcaga tactgcaaga cttttcgtat tatttgcagc accaccagaa
    14281 agagatttag actggtcaga gcaaggtgtt gatggatgct ttagattctt aaatagggtt
    14341 tatagattgg ttgacgagtt agcagatgtg gttaaaaagg atgtagagtt tggagaatta
    14401 aattcccaag ataaggatat gagatatact atacactcta cacttaaaaa agttactgct
    14461 gatttaagtg aaaaattcgg atttaatact gctatatcag ctcttatgga attaataaat
    14521 gatatgtata aatataaaga gttagataat ataaacgaag cagttataaa agaaggtgta
    14581 caaactatag taactataat tgcaccattt gcccctcatt taggtgaaga actatggact
    14641 atgataggta aagaaggaag tgtatttgat atagactggc ctaaatatga cgaaaaagct
    14701 ttagtaaaag atgaaataga agttgttgta caagttaatg gaaaagttag aggaaaatta
    14761 actgttaact caaatatatc taaagatgag atggaaaagg tagctttaga agatgaaaaa
    14821 ataaaaggat tagttgaagg taaaactata gttaaagttg tagctgtacc taaaaaatta
    14881 gtaaatatag ttgttaaata actcaaaaat atgaacaaaa atataaattt ataaaataaa
    14941 tgaggctgtc tcaaaataga gataagcctt ttttcattgt tgaaattaca aaaatagatt
    15001 tgattttata caaaaaaatg attaaagaac taaaaagttc attcttatac actctaattg
    15061 ttgttttaaa tattattata attaatgtta aatcgtgtta atgtattaag tggtatgtaa
    15121 attattcagt ttctaaagtg ttgttcatca aggaactgct ctaagcttaa acgaaaaaaa
    15181 tataattatt gtatttttaa aatttttttt atataatgaa aacaggcaaa aaaggtgcgt
    15241 atctatgctt tatcttaaac acccagacaa cgagattcag attcatataa taactaccat
    15301 gtttctaaaa aggaacttgc aaaaaagata ggcgtttcaa cttcccagtt gattaaaatt
    15361 atcaccagga aaacaaggat ggtcagcagt agtattctaa tgggtgttgc tgatatatcc
    15421 aagttgggat tgtttgagga tgttatcaaa tgacttgtga taggtgcagt agatgtggag
    15481 attcctgata cggatttatt ctcaggggat gtttgcttca ggagatatgg agcgtaacca
    15541 gtttattgat atggtaacca tttttttgtc taatttgatg aaagaatccc aagaatacca
    15601 tacagaagta agacaggaat taactggtgg tataaactga taaaggcaaa tttaaaaatt
    15661 ttgataggat gattttaaga tttatcttct attttgtttt ttagaaataa ttgaaatgtt
    15721 atgagggggt gtgtacaaat ggagactgta tcaaataatc aatcaagtgt agaaattcaa
    15781 ctcagtctgt ttgaagctat caaagattta ttgttaatta atcctatttt cttgttggga
    15841 attatcttga ttggtatttt cttagttgta atattcaaga aaaatagtga tattccaaag
    15901 gtaaaaacat ctattttatc actggcaatg tattactact tatgtttaac actatcgcat
    15961 attgttggta tccctacatt cagtgaatat ataagactgt cacagttagg ggaaacgttc
    16021 tttaatccaa acataaatct tattcctttt agtgatggat ttagtttgag ttttattttg
    16081 aatattttac tttttgttcc attaggtttt ttatgtccgc ttatcagcaa aacctttgaa
    16141 cggttgagaa atacattctt gattggtttt ggattatctc tctttatcga aactattcag
    16201 ctttttacgt tacacagagc aaccgatata gacgacttac tcacgaatgt aattggaaca
    16261 ataataggct atttctgctt caaactgatt gctaaattgc gaattgtaaa actacattca
    16321 gatgatgact ctatggaaca ggattacaca gtatgtattc cgattgccat tataatagtt
    16381 gcccttatat taggattttt tagttaagtt ccaaatagct aatttgggaa tccagacaat
    16441 ataacagata gtatgttacc ttttacagga gcatactgtc tgttatgttg tcaacatctc
    16501 attttatggt ttgtgtgtat tttcttgtaa actgaactaa ataattaaat agaagttatt
    16561 agaattagca atagattcct ttttcaaaga aatctttgat ttcatcaagg ttatcagttt
    16621 ttccaagtgc taagataagt tttattctag ctttttgacc tggcaagtcc ccaccaaata
    16681 tacaaccaag attttttaaa tctctaccag aaccaaagta accataacta tcgaatactc
    16741 tacctgaatg acatctagaa actataacta caggaagtcc tttttctcta gcatattcaa
    16801 ctccagaaag catttgagga ggaatattac ctatacccat tgcctcaata actatacctt
    16861 tataaccact atcaacagca aatcttataa agtcagcatt ttctcctaca tatgctttaa
    16921 ataaggcaac tttagattct accttatcag tatctataat tgttctattt acaatatctc
    16981 ttgtaaggac aagttcgttg cagtcaatta ttcctaatgg accactagtt agagatttaa
    17041 atgtatttag agaaagagta tttgttttag tagcttcaga agctaataat acttcattgt
    17101 ttaaaactac aaggactcct tttcccatag catctttgga tactgcagta caaactgatg
    17161 cagataaatt acttgaacca tcatatccaa gttcagaact acttctcata gcacctgtga
    17221 ctattacagg tttgatgttg tcaattgtaa ggtctagaaa ataagctgtt tcctctaaac
    17281 tatctgtacc atgtgttatt acaacacctg taatatcttt tcttgctaat aaatcattta
    17341 cataattttt caattccatc attctataag gtgttatgtg aggtcctgga atttcatcaa
    17401 aattaaaaac ttcaacatct gcaactttat caatgttggt aaccattgat agtatttgtt
    17461 cacctgataa agttggtata gctgctccga ctttatcatc tactgtcata gatatagttc
    17521 caccagtaaa aactatggca acttttttct taatactcat acgattctct gcctttcata
    17581 aataaaattt ataattccga taggattata tcaaaaaaag tagttaaggt ctacataaac
    17641 tcttttattc atataattta gatatttcag tgtaatataa ttatttctta attttttgcc
    17701 attcaacgat ataaccattt aatatctctc ttatggcttt tcctatctta gtataagctt
    17761 catcatatag atttgctaga gatattctaa ttgaccattc agggccatag aatccatttc
    17821 cactaagtaa aactactgaa tagtcattag ctaatttata aagaacatct actggagatg
    17881 cagttttact tataaattta aataagtctt ctccgtagtt taatttagcc cattcttcaa
    17941 tatcaaattg agtgtaatat gcagcattgt ttttgttttc attaattgga agctctaaag
    18001 cttcaaataa tagtttttga cgtctatggc agatactcat attgagtttt ttgtaggcat
    18061 ctactttgtc aatcaatgca aaagcagaga aaaacgccat ttgtacttgc tgtggagtag
    18121 aaagaccagc agtatggttg agagcaacaa gtctgctatc agcaacaact ctttccataa
    18181 atgaaagact agaagggtta agactcatat cactgtaacg cttatctact gattttttta
    18241 attctccagt taaatcattt attttcttat caaaaacatt tttcttatga agggcaaatg
    18301 taccaagtct ccatcctgta actccaaaat attttgaata agaatatact ccaacagtgt
    18361 tgtaaggtag ttttgacatt aaggatgaaa aatcttctac aaaagttcca tatacatcat
    18421 ctgatattat cattaaatct ttattataat tttcaattac atctattaga ttattacaag
    18481 atatttcatc catagctata gaagcaggat tattaggatt aacaacgaat aaagctttaa
    18541 tagatttgtc acgaagtttc tctaattctt tttttgtata ttgccatgta tgagcacctt
    18601 tttcatcaac ttcattagca tttatattta ccactttaaa gtcgtaacga ggaaggttag
    18661 gtatttcaag ataaggtgta aatattggag tcattaaggc tattgtatct ccttttttta
    18721 gaagttcatt tgccattaat gaatcaaata tatagcacat agcagctgta gcaccttcaa
    18781 cagcaaaaat ttctacatca ccatatttac ctgatatatc atatttcatt tcttgaacta
    18841 ggtaatcgtg tactattttt tccatatgag gtaacattct atctggaagt ggatagttat
    18901 caccaataat tccatcagct agttcatgaa caaattcatc tccatcaaat ccaagttcat
    18961 ttataccaaa gtttataata tctctagcta aatatgctcc aggcatatct atattttcat
    19021 ttataaattt aaaaaatctt tctttaatac cctttttttg aggcattcca gccaaatgac
    19081 ctaaatccca attagaacga gtttcagtaa tagcgaaatg accaaaagta aaaaaggctt
    19141 ctctagcagt tgaacaagtc cagtttggat ttccacgacc tgcatcaagt aattctctgt
    19201 tattttcttt tgcagtgtat tttgcaatat ctattaattt gtccttaaat tcaaaagggc
    19261 ttatttttcc atatatgttt ttgatttctt tataagtatg atttttcatg ttaaattcct
    19321 ccttatgtaa taagtttcac atttttataa taaaataaac aagttgcaaa aatgattata
    19381 ctttaattat aataggttta tagaaaaagt aaagtatgaa attaaaataa taactctatt
    19441 tagccttatt aaatatatgt taaaataaaa aatacctttt aataaaaaat gattgttata
    19501 taaaataaat attcaattta attatcacct tagttttttt attgtatttt attatatatg
    19561 tatagttttc taattttgaa taaaaataaa gtaaaacttt cttttatatt gaagttgttt
    19621 agtaagacat gtattttatt aatttattaa tcttaataat gttaaataaa atcaaaaaaa
    19681 tagattttgg gtaaataaaa taatataaaa tat
//
LOCUS       DAEPOG010000064        19598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEPOG010000064 DAEPOG010000000
VERSION     DAEPOG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.064"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1754
                     /locus_tag="KR432_001879"
     CDS             <1..1754
                     /locus_tag="KR432_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0640416.1"
                     /translation="KIGVVKNLIDCYKTEQNSLMLLKSNFLLALLYGIQGFSEKMKEY
                     ILISCKYIDKCLPKDYKFLARFYSQIAILSLKNEDVNKANLYMDKFNLICDENNFLIE
                     EIIFKSQLIYIKASKCIESSVIIKEIETLYIKIKEINDFNCKKIYFFIIGKIYFLFLD
                     DALIAKVNFLKARKYADLLKDMQVSSLCNIKIGECECSFENYECSIEYLNEVISNKKY
                     RNVNIIQKYRASNNITKILIKTRNYPVAINNLAKSEVYLEKIKNQSLKEVEKFDLFIS
                     LAMYYAKSNEKSFEQSICYLDRAKNILNMIKNIEEYTIYDLEIIYHQAKIYYIFGYYD
                     NALNTNKKLLEKSKNAKNYNYVKLAYKNIYLCFEKIQNYEMSMKYFKMYYELKMMYIK
                     ARNRNYIDSLNYRHEYIETEINNMRKVKLDLDKKKYVDHLTSAYNRTFLNDFLEKQEV
                     SEYCTAFMIDVDYFKEYNDTYGHYNGDIALKNITIIIKKYLKKDMFMIRYGGEEFLIL
                     SVCKDYKKSKIFGRKLCRIVKKFLNNNLTVSIGIDTCKNSSIDIREIIENADKALYKA
                     KESGRNRCLHYHDFKNF"
     gene            1781..3667
                     /locus_tag="KR432_001880"
     CDS             1781..3667
                     /locus_tag="KR432_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0640417.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            3675..5675
                     /locus_tag="KR432_001881"
     CDS             3675..5675
                     /locus_tag="KR432_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640418.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            5937..7196
                     /locus_tag="KR432_001882"
     CDS             5937..7196
                     /locus_tag="KR432_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0640419.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            7257..9044
                     /gene="iorA"
                     /locus_tag="KR432_001883"
     CDS             7257..9044
                     /gene="iorA"
                     /locus_tag="KR432_001883"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBH0640420.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            9046..9621
                     /locus_tag="KR432_001884"
     CDS             9046..9621
                     /locus_tag="KR432_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBH0640421.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            9674..10744
                     /gene="buk"
                     /locus_tag="KR432_001885"
     CDS             9674..10744
                     /gene="buk"
                     /locus_tag="KR432_001885"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0640422.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            10962..11663
                     /locus_tag="KR432_001886"
     CDS             10962..11663
                     /locus_tag="KR432_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640423.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            11722..12228
                     /locus_tag="KR432_001887"
     CDS             11722..12228
                     /locus_tag="KR432_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBH0640424.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            12403..13425
                     /locus_tag="KR432_001888"
     CDS             12403..13425
                     /locus_tag="KR432_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0640425.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(13572..13880)
                     /locus_tag="KR432_001889"
     CDS             complement(13572..13880)
                     /locus_tag="KR432_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0640426.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(13940..14368)
                     /locus_tag="KR432_001890"
     CDS             complement(13940..14368)
                     /locus_tag="KR432_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640427.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(14591..16048)
                     /locus_tag="KR432_001891"
     CDS             complement(14591..16048)
                     /locus_tag="KR432_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0640428.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            16466..17380
                     /gene="nadA"
                     /locus_tag="KR432_001892"
     CDS             16466..17380
                     /gene="nadA"
                     /locus_tag="KR432_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH0640429.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            17393..18694
                     /locus_tag="KR432_001893"
     CDS             17393..18694
                     /locus_tag="KR432_001893"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBH0640430.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            18675..19511
                     /gene="nadC"
                     /locus_tag="KR432_001894"
     CDS             18675..19511
                     /gene="nadC"
                     /locus_tag="KR432_001894"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH0640431.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         7737 a         2005 c         3488 g         6368 t
ORIGIN      
        1 ataaaattgg tgtagtaaag aatttaattg attgctataa aactgaacaa aatagtttaa
       61 tgttattaaa atcaaatttt ttgctagctt tattatatgg tattcaaggg tttagtgaaa
      121 aaatgaaaga gtatattttg atttcctgta aatatattga taaatgctta cccaaagatt
      181 ataaattttt agcacgattt tattcacaaa tagctatttt aagtttaaaa aatgaagatg
      241 taaataaggc aaatttatat atggataaat ttaatcttat ttgtgatgaa aataattttc
      301 taattgaaga aattattttt aaatcacaac ttatttacat taaggcaagt aagtgtatag
      361 aatcttctgt aataattaaa gaaatagaaa cattgtatat aaagataaaa gaaattaacg
      421 attttaactg taaaaaaata tattttttta ttattggtaa aatatatttt ttgtttttgg
      481 atgatgctct aattgctaaa gtaaattttt taaaagcaag aaaatatgca gatttactaa
      541 aggatatgca agtatcttca ctatgtaata taaagatagg tgagtgtgaa tgttcatttg
      601 aaaattatga atgttccata gaatatttaa atgaagtaat aagcaataaa aagtatagaa
      661 atgtaaatat aattcaaaaa tatagagcat caaacaacat aactaaaata ttaataaaga
      721 ctagaaatta tcctgtagca ataaataatt tagcaaaatc tgaagtatat ctagaaaaaa
      781 ttaaaaatca gagcttaaaa gaagtagaga agtttgattt atttataagt ttagctatgt
      841 attatgcaaa aagtaatgaa aaatcttttg aacaatcaat atgctattta gatagagcta
      901 aaaacatctt aaatatgata aaaaatatag aagaatatac aatatatgat ttagaaataa
      961 tttatcatca ggcaaagata tattatatct ttggatatta tgacaatgcc ttaaacacaa
     1021 ataaaaaact ccttgaaaaa tctaaaaatg ctaaaaatta taattatgtg aagttagcat
     1081 acaagaatat atatttgtgt tttgaaaaga ttcaaaatta tgaaatgagt atgaaatact
     1141 ttaaaatgta ttatgagtta aaaatgatgt atataaaagc tcgaaataga aattatatag
     1201 attccttaaa ttatagacat gagtatatag aaacagaaat aaataacatg agaaaagtaa
     1261 aacttgattt agataaaaaa aaatatgttg accatttgac aagtgcatat aatagaactt
     1321 ttttgaacga ttttttagaa aaacaagaag ttagtgaata ttgcacagca tttatgatag
     1381 atgtagatta ctttaaagaa tacaatgata cttatggaca ctataatgga gatattgctc
     1441 ttaaaaatat aactataata ataaaaaaat atctaaaaaa agacatgttt atgataaggt
     1501 atggtggaga agaatttctg atattatctg tatgtaaaga ttataaaaaa agcaaaatat
     1561 ttggaagaaa attatgtagg attgtaaaga aatttttaaa caataatttg acagttagta
     1621 ttggcattga tacatgtaaa aatagtagta tagatataag agaaataatt gaaaatgctg
     1681 ataaagcttt atataaagca aaggaaagtg gtagaaatag atgtttgcat tatcatgatt
     1741 ttaagaattt ttaactgttg aaataggatt ggtgatttac ttgaaaagga gtttaataga
     1801 agatttaaag agaaatataa acaagataga atacgatatg ttgactaaat tctatctaaa
     1861 tttgacagat ttaaactaca tcttggatta tcacaaagac cttttaaata caaaactaga
     1921 agataatagt agagtctata ctttatattt aattagcctt ataaactata taaaaaaaga
     1981 tagagaagca tcatacaact atttgataga gtcattaaag tatattaaaa aattgtcatt
     2041 aaaagaagat tcagatataa tatctaaaat ttatatttat cttactttat cagctatttc
     2101 tataagaaaa tatgataagg caaataattt ttatttctca gcaaaaaaag ttataagaca
     2161 aaatcattta aatgagttgc tagtcttgtt aaatatgagt ttatgtgtag aaataagtgc
     2221 tttatatcag accaaaacgg atgtaatagc tttagtagaa gaagtgtctt tttttaaaac
     2281 tttaaaaaat aaagctcttg taagtagggc aaattatata tttgggagag tttatctata
     2341 tttatttaat aactttataa aaagtatgga gtacctaatt gaatgtctaa aactggctca
     2401 agaaaataat ttatattcta tagaggcttt ttgtaaatgt ataataagtt tgtgttattt
     2461 agagagtgga aataatgtag aagctataaa atacataaat aatgtattag atgttgcgca
     2521 cagtaagaac tcaatatcag taactgaaag agtgtctata aagatagacc ttatatgggc
     2581 atatttaaaa gaagatatga ataaagaagc agaagttatt ttattaaaga ctataaaatt
     2641 aatgaaagtt gttgaaggtg tatataagga ttatttatat tcatttatat ttttatattc
     2701 agctgaaatt gagttaagga aagataattg tagttttgaa aaggtaaata cctatttaat
     2761 tttatcaaaa aatatatata aaagttttgg cgatgttttt atatataatt ctaaactaga
     2821 ttacatatat aagttgtatg gagatttata tataaaattt ggtaatatag atgaaggtat
     2881 aagttgttat ataaaaggtt taaatttagt taaaaagagt tcattgaatc ttagaaaaat
     2941 atctatattt tatgggctaa tagcaaatgg atatgagcta aaaaaagatt ttaaactggc
     3001 aatagaatat tataaaagta tggattggta ttctgatagg tgggaaaaaa ataattccta
     3061 taaaacatct caagcaattc acaaacaata tgaactcaga caaaaacaag agagtttaag
     3121 attactagtg gatgataata agaaattaga aaatgatatt ttaaaagatg gacttactaa
     3181 actatataat agaagatatt tggaacagca gttggattta tttaatggaa ccagagacaa
     3241 aaatatggtt gcaatactag tcgatataga ttgttttaaa agttataatg ataattatgg
     3301 tcatctagca ggagataaag ttatagttaa ggtaactgat attataagaa gtgtatttat
     3361 agatattacc gaacatataa tcagatatgg gggagaagaa attttaatac tagtagattc
     3421 tatagaatca atcggtgttg aagagattga taatatagaa ttatatatta aaagagtttt
     3481 caaacttctt gaatttgagg gtattgaaca tatatattct aaaataaaaa actatataac
     3541 tataagtgct ggagtaagta taaaaagatg tcgtagtaga aaagatgttg aaatattaat
     3601 tgaagatgca gataagaacc tatataaatc aaaaaaagca ggtagaaatc aatacattat
     3661 aatttaaaat tgaggtgatt ttgttgcgaa aaaaaatagg aattatagcc tctgatattg
     3721 aattaaaaga aagaatagaa gaattatata gagaagatgt agaaaatgga actataataa
     3781 tcgacatact taacctagac ttaatggaaa atcaaggtcg tatacttgtt gaaaaaggtg
     3841 cccaagctat aattggtaga ggtggaggtt atagtttagt aatagataca gtaaatgtac
     3901 ctgttatacc aatgaatatg aagtcaactg accttttaag agcaatagaa atagcaaaga
     3961 aatattctaa aaaagtagta ttgattcttg gagacaatga agtttctttt gactatgttg
     4021 gctggagaaa tgtcataagt actgaaataa ctgaagaatg gtttgaatct aagtatgaga
     4081 taagaagtaa agttgtaaaa tatatagacc aaaaagatga agttgtaatc gtaggaggag
     4141 gtttggcatg ttcttttgca aggcaatatg gaattgatag tgtatttgca actgctagtg
     4201 atgaatctat aagagaagct gtagaatatt gtaaaaaatt attggataca ttaggtgaag
     4261 aaaagtttaa taatgaagtg ttacgaaata tacttgatgg tataaaagat ggagtaattg
     4321 ctatagatag taatggtagt ataattttgt acaatgaaag tgcaaaaaac atgcttaaag
     4381 tagagagaaa atgtgcactt aataaataca tcttagatgt atttcctaaa atggagtgga
     4441 tgttagattg tttacatgaa aaagaggatg tagaagatag aaagataaga aatataaata
     4501 atctaattgt taatactagg acaacattaa taaaagttga taatagtact tatggagttt
     4561 taggaataat acaagatata accaagctac aaaacttaga aagaaaaata agatttgact
     4621 tgaatcaaaa aggtctctat gctagatata cttttgatga ttttttattt aaagataaat
     4681 taactagaga atttattgaa gaagctaaaa aaattggaaa aagtgattat acaactttac
     4741 tttatggaga aagtgggtct ggtaaagaaa ttatagctca tagtatacac aatataagta
     4801 aaagaaaaga taggcctttt gttgctataa attgtgctac tatagcagaa aatttacttg
     4861 aaagtgaact ttttggctat gaagaaggtg catttactgg tgctagaaag ggtggaaaaa
     4921 gaggactatt tgaacttgct catggaggga ctcttttttt agatgaaata aatagtctat
     4981 cctttaatat tcagacaaaa ctattaagag taatagagga acgtcaaata atgagaattg
     5041 gttctgatta tataatacct ctggatataa ggattatagc agcaacgaac gaatctttaa
     5101 ccgaaaaaat agttatgggt acatttagag cagacttatt ttatagattg agtagtttag
     5161 agataaatat acctccttta agagacagaa gagaagatat aatacctttg tttaacaatt
     5221 ttgtaaatga agtattaaag gatgatggtc tcaatggaat aaacagtata gatgaaaatt
     5281 ttgttttgac taaagatgaa atggataagc tatataatta tagctggcct ggaaatgtaa
     5341 gagagttaaa gacaatagct caaaaatatg ttgtgacagg taaaattaaa ttgagacaag
     5401 atagagattt taaaactaaa aaattacttc ctaactctga agtagacaaa tttaattctg
     5461 aaacgacagc aagtgtagaa gtccaagatg aatctataaa tataagtaag ataaatgatg
     5521 gtaagataag tatagatata aaagaagtaa ataagtatgt tgaagaaaaa ataataagta
     5581 tgctttttgc tcagggactt tctaaaaatg aagttgcaca agttcttgga ataagcagga
     5641 catctttatg gaagaaatac aataaaaata tttaaatata attacatata aattattttg
     5701 aagtaatgtt aattttttaa aacaagtgtt aagaaaaatt aacattactt ttttgtaaat
     5761 ttttattttt ttagacaggc atatatattt ataagaaaaa tagttaaaac acttaattta
     5821 ataatgaaaa aaataataat tctaaacaaa atcaatttta cataaagtgg gatttattgg
     5881 catagtaatt gcttaatata taatgtaaga gtaaatacaa ttttaggggt gatggtatga
     5941 gtcaaagtat ggttggtaag catgcaatgt ggccaaaaga aaatgatgtt atatttagca
     6001 tatctggtag agctcaagca gcagagaagg cttttggtat ggataatgta ataaatgcaa
     6061 caataggcgc tctaatggat gattcaggaa aattaataac aatgaaaact gtatatgaag
     6121 aatataaagc attagataac tgtgagatag gtgcatatgc agctcttgaa ggtcaaccag
     6181 actacttaga agcagttaaa aaagtatttt tcagagatta cttaccagaa ggacatataa
     6241 gagtattagc ttcaccagga ggaagtggtg caataaaatt agctgtatgg aactatacta
     6301 atgaaggaga tgaggtatta acatctgatt ggttttggag tccatatgta agtatagctg
     6361 aagaggctaa tagaaaagta gttaattacc aactttttga tgaaaataga aggtttaatt
     6421 ttgagtcgtt caaagaaaaa tttgtaaata tagcagaaaa acaaggtaga gtctttacaa
     6481 ttataaacac accagcgcat aatccaactg gatatagtgt tgcagatgat gagtgggata
     6541 aaatattaga cttatcaaaa gaagtcgcaa aagataaaga caaaaagata atatttttcg
     6601 tagattcagc atatattgac tttgcaggtg atgatgatgt atgtagaaag ttttttaaga
     6661 aattttctaa tttgcctgaa aacgttctcg tattggttgg attcagtatg tcaaaaggat
     6721 ttacagctta tggtatgaga atgggggcta taatttgtat atcttcaagt gaagatgtag
     6781 ctgaagagtt ccattactca tgtgttcatt catgtagagc aaattggtca aattgtaata
     6841 gaagtgccat ggcagtatta tctaacattg taaatgaccc taaaaaattc aaagaatatg
     6901 aggatgaaaa agaaatatac aaaaacatgc ttactagaag agcagatgta tttgtagaag
     6961 aagctgaaag agtaggatta gaaattcttc catatatagc aggtttcttt gtaagtatac
     7021 catgtgataa tccaaaagaa gtatgtgaag aattgactaa acataattta tttgcagttc
     7081 cattaaaaat gggcttaaga tttgcagttt gtgcagtttc tgaagataaa tgtaagaaag
     7141 caccaagtat tataaaagaa gcacttgaaa gtttagaagt aaaaataaat aattaatttc
     7201 attgaacaaa ttgggtggac tatgaattta ataattgtag atggagggaa ttttatatga
     7261 aacaattgat gacaggtaat gaagcaattg cacgtggagc ctatgaggct ggtgttaaat
     7321 atgcatctgc atatccaggg actccaagta cagaaatatt agaaaatata gcaacatata
     7381 aagatgcaat agttgcagag tgggctccaa acgaaaaggt agcacttgag gctgctatag
     7441 ggggttcaat tgcaggagca agaacaatgg cttctatgaa acatgttggt ttgaatgttg
     7501 cagctgatcc aatatttact tatgcatata caggggtaaa tggtggtatg gtacttatta
     7561 cagcagatga gccgggaatg cattcatctc aaaatgagca agataatagg atgtatgcaa
     7621 aatttgcaaa gataccttta tttgaaccat caactagtca agaagctaaa gatatgataa
     7681 aagaagcttt tgaagttagt gaaaaatacg atacaccagt tttatataga gttacaacaa
     7741 gactttgtca ttcaaaagga ttagtggagt gctatgatag agaagaagtt gaaattaaag
     7801 agtatgttaa aaatgctaag aaaatggtaa ctgttccagc aaatgctcaa ataagaaggg
     7861 gcgttgttga agaaagaatg gaaatcctta aaaaattctc aaatgaaact gatttaaact
     7921 actatgaaat gaatgataca aaaataggtg ttatagcttc tggtatgtgt tgtaattttg
     7981 ccaaagaagt ttttggaaaa aatgcatcat atatgaaact aggatttaca aatccattac
     8041 cttatgaaaa aataaaagaa tttgcagaaa aagttgataa aatatatgtt atagaagaaa
     8101 atgacccatt tatagaagaa caaataaaag catatggaat agattgtatt ggaaaagatg
     8161 taataccacc atatggagaa atgacaccag atgttttaag aaaagctata tttggaaaaa
     8221 caaatgatac tatagaatac aaatctgaat tagtaacacc aagaccacca agtttctgtg
     8281 caggatgtcc acatagagga ttcttctatg aacttggaaa gagaaaaaat ttaatagttg
     8341 gtggagatat aggttgttat actcttggat ttgctcctcc atataatgga atagattatg
     8401 ttgtttgtat gggttctgct tttggaactg cacatggagc tcaaaaagtt cttaatatga
     8461 aggatgataa tgaaaaaaga ttggtaggtg tacttggaga ctcaacattc ttccatacag
     8521 gaataaatgg acttttagat gttgtttaca atagaggtaa ttctatttcg gttatattag
     8581 ataatagaat aactggaatg acaggtcacc aagaaaaccc aggttcaggg tatactttac
     8641 aaggtgctaa aactaaagaa gttgatatag aaggattagt taaagcttgt ggaatagaac
     8701 atgtaagagt tataaaccct aacaatttaa aagaagtaaa tgaagcacta gattgggctt
     8761 tagctataga agatgaacca tcagtaataa taacaagatg gccatgtgta cttaaaaaat
     8821 tctctaaaga agatatagaa gaatttaata atccatttaa gactaagtgt aaagttgacc
     8881 atgataaatg tataggatgt aaattatgtt tgaaaactgg atgtccagct ctatcatttg
     8941 ataaagagaa taagttatca aatatagata gaaatcagtg tgtaggatgt ggagtatgtg
     9001 cacaagtttg tccaaaacaa gcaatagtta aggaggaaaa ataatatgac taagagtata
     9061 cttttggtag gtgtaggagg tcaaggaact atacttgcta gtaaattatt aactatggga
     9121 ttgatggaag ctggatatga tgtaaagatg agtgaaatac atggaatgag tcaaagagga
     9181 ggttcagttt cctcacaagt tagatatgga gattgtgttc attctccagt tatagaaatt
     9241 ggtggagcag atatattagt atcttttgaa aaaatggaag ctttaagatg gttcaattat
     9301 ttaaaaccag aaggaaaagc tttagtaaat aatcatagga tagattctat gactgtttta
     9361 atcggaggag ctgaatatca agaaagtgat atagatgcag agttaagtag attaaatgca
     9421 aaagttataa atgcagctga taaagctgaa gaactaggaa attcaaaaat aatgaatgtt
     9481 attttattag gatgtctagt taaatctatg caattagagt ctattgattg ggaaaaaatt
     9541 ataagtgaaa atgttaaacc aaaatttgtt gaattaaaca taaaagcttt ccatgaaggt
     9601 atggaaatgg ttagcaaata gtttataata gatataaata gactatagaa attaaggtga
     9661 aaggaagaaa attatgactt acagaatatt agccataaat ccaggttcta cttctacaaa
     9721 aatagcagta tatgatggag aagaacaaat tcttgtgaag acgatagacc atccagctga
     9781 agagattgca aaatataata ctatacaaga ccagtttgaa atgcgtaagg aagcagtttt
     9841 gaatattctt aaagaaaata gtatagactt aaaatctctt agtgcaatag taggaagagg
     9901 tggagtttta ccaccagtaa aatcaggagc atatctagta aatgaagaaa tgattgatgt
     9961 actaagacat agaccagtac ttgaacacgc ttccaattta ggtgctgttg tggcacatgc
    10021 aatatcagaa cctcttggaa tcaactcata tatttatgat tctgttgcag tagatgagct
    10081 tatagatgta gcgagaatat ctggactttg tggaatggat agatcaagtg cagggcatgc
    10141 attaaatact agagcaatgg ctttaaaata tgctaaggat aaaggaaaag attataagag
    10201 cttaaactta atagtagctc acattggtgg aggagtaagt atttatcttc atgaaaaagg
    10261 aagaatggtt gatatgctat ctgatgatga aggaccattt tctccagaaa ggtcaggaag
    10321 agtacctgct acaaaattag tggctgcctg ttattcaggt caatattcag aaagagaaat
    10381 gactaaaaag ataagaggta aaggtggtat agtttcatac ctaaatactg tagatgctag
    10441 agaagttgaa aaaatgatag cagaaggaaa tgaagaagca aaaattattt atgaagcaat
    10501 ggcttatcag ttagcaaaag gtattggaga gttagcaact gtagtagatg gaaaggtaga
    10561 tgctataatt ataacaggtg gaattgcata ttctgaaatg tttacttcaa tggttaaaaa
    10621 gaaagttgag tttatagcac cagtagaaat tatggcagga gaaaatgagt tggaatcact
    10681 tgcttttgga actttaagag tactaaatgg agaagaagaa gctagaattt atagtgaaaa
    10741 ttaataagaa aaattaaaaa tgtttatata ctaaaaaagt ggtatatccg ttttttatac
    10801 acgaaagctt agagtatagg tgaaaatgta taaaatttgg attgccactt tttataatta
    10861 aaattttatc ttaatctaat aaataattct gtaaaatttc gtatttatat atatatttta
    10921 ttggtattta ttgttattat tattatgagg agtgattttt gatgggaaaa ataagtttaa
    10981 aaagtatgat aagattattt tcaggttttt ttatatttgc aatttcttct gtgcttatga
    11041 ttaatgcaca tgtaggtctt atgccttggg atgtacttca ccaaggttta tctataaagc
    11101 taggcataac tataggtcag gcttctataa tggtaggagt tgtaattgtt atattggatg
    11161 cagtttttgg tgaaaatata ggatggggta ctttactcaa catgacattt ataggaattt
    11221 ttattgattt agtaatattt tctggagtga tacctcatgc aagcaacact tatataggtg
    11281 tgtttatggt tgttatagga attattttgg cagctatagc aagttttcta tacttaggtg
    11341 tttgtttagg aagtggaccg agagatgggc ttatgattgc tcttcagaaa aaaactaata
    11401 aatctgtacg tcttgttaga acaattcttg aaatactagc attagttgtt gggtggttat
    11461 taggaggttc tgtaggtatt ggtacgcttg taagtgcttt aggtttagga tatgtattac
    11521 aaatagtgtt tagaatattt aaatttgaca caaagttgct gaaacataga tttattatag
    11581 atgatataag agaatggaag gaaaagagaa gcaatgaaca taaatgtaaa tcaagtattg
    11641 taataaaaaa tgagcagaat taattatgct aaaatatatt tgtagtttat ctaatttata
    11701 gtaaagtcag gagattttga aatggaatta tgggatttat ataatgctga tggaattaaa
    11761 acaggaaatg ttataaaaag aggtaattct attgaagaag gttattacca tttggcagtt
    11821 gaagtgtgga ttttaaatag taactctcaa attttaattc agaaaaggtc taaaagtaaa
    11881 aagacattgc caaatatgtg gggaatgaca actggatgta ttgtttctgg ggaagatagt
    11941 ttagaaggag caattcgcga agcaaaggaa gaaataggga tagatataac taaagatgag
    12001 atgaaagttt ttagaagtat gattcatgaa gatactcttt gggatgtata cttagttaaa
    12061 aaagaatatg atatatccaa agctatttta caggaggaag aagtaagtga tataaagtgg
    12121 gtaagcacag atgaaataag acaattacta aaagagggtt tgttttttga atatccagag
    12181 atgtacgaat tgctatatga gatagataat aataaattaa atatatgatg tagagcagat
    12241 ttcagtataa gtaagattat ttaaaatgta tcttctctaa attttaatat tgttttatga
    12301 aaattaataa gttattttta gtatttttct acttgttttt tgagattaaa ataaaaaggg
    12361 cagtcattat aaagtcataa aaatttgggg aggatttaaa ctatggaaga taacaaagga
    12421 ttggcacgtg atagttcaaa atataaaatt gtttctatat ctgtcgtagt aattattcta
    12481 attacatttt ggtatatgtt aaatatggtg cttttaacat ttattatgac ttttattttc
    12541 tataacttgc ttgtagccac aagaaaacga attaagaagt tttcttcttt aaatattcca
    12601 gattcattga taattatagt gttatatgca ctatttgcta tattgttagt attgattagc
    12661 tatgcagttg taccaataat tatagttcaa cttacagagc tgagtagggt ttttagtgat
    12721 tttgatgtaa atcaatttgc tcaatcttta ggaccaaaat tgtatcctat tgtctcaaag
    12781 ctggatttta ataaatatat ttcacaagca gggctactta tagcatcaac agcaacaaaa
    12841 gttggaagtt ttggagtaaa tatattattg gcatttttac tgagtttact ccttttactt
    12901 gaaaaaaatg aaattaaaaa ttttggagat aaactttctg atagtaagat ttcttttatt
    12961 tataattcgc ttgtattttt tggaaagagc tttgttaaaa attttggtga agtaatgaaa
    13021 gtacaagtta tgattgcctt tataaattct gttgtttcta tgattttctt aggtttcatg
    13081 ggattccctc aaatatgggc tttaggattt atgatttttg tgttgggtct tattccagta
    13141 gcaggagtta tagtgtcttt gattccatta actgtaatag catttaatac aggaggaata
    13201 acaaaggttt ttggagtttt acttatgatt tgtatagtgc atgcagtaga aacatacata
    13261 ttaaacccta agttgatgtc gaatagaact aaacttccag tttgttttgt ttttattatt
    13321 ttacttgtag gagaacatta tttaggtgta tggggattac taattggagt tccaatattt
    13381 atgttcctta tggatattct gggtgttaaa tttacttcta ggtaatatat taaaataata
    13441 atttattttt atatataaat tatgaattgt tttaaaaaag aacctataaa tagacatcat
    13501 taataaagac atctatttga taggttcaat tttgctttgt ataaatacta tagaattaaa
    13561 taaaaagaaa tttatttcat ttcaaaagtt tcacaacttg catttctttc atttatatga
    13621 actttttcag ctttgcaaag ttcattttta ttgtatttac attttacagc ttcacaatga
    13681 atgttatctg tatcaacttg gttagtttca ttagaacgac tagttatacc agatgtatat
    13741 ttgtcttcaa aagaagcaca ataagtattt tctgtagtag ttgcttgcat tccatctact
    13801 tttatactac cagcataaca atatccacta gtattataag cacaattagt agcagcacaa
    13861 tttaaattat tatttttcat aaataacctc ctaagtttta tttaaactta ttatgtgaag
    13921 ttatctaaat tttatacact tatttacaat tacatttatc acatttaaca actacatctt
    13981 tggaagtttc ccttcctaaa tttttattaa catgatacac atcaacattt tcagttgatc
    14041 cacatataga acatttaaaa ttgttttcta gtagggttct ttctttaaat aacttccaat
    14101 acatagaatt gatgtaatca ttataattta ccttgaggca tgtctcaaga aagtttatgt
    14161 atagattgtc taaatctcct gtatttaatt ctgtatattc tggttcaaaa tattcacaac
    14221 atcctctaaa atcaggcttt gtatctgaaa taacacatct aatatcatat ctatagtcta
    14281 ggctaggccc cttaaatgaa agatgtgtgc aaaaagaaca agatgtttct ggtagatttt
    14341 tttcaagttc atgtaattta ttattcataa gtattcctcc aaaaaattta ttttaagtat
    14401 ataaatgaat agattatttt aaaactttta tttatttata ttagagattt aggataaata
    14461 ttccaataag gagtaaatac ttaagtattt attactgtat taaataaaaa agaggcaaat
    14521 ctaacttgag attgcctact tttttatcat tttttaataa aatttattct aagtattatt
    14581 ttatttagat ttagtcagcc aatttagtat ccgctttgct ttttaacttt ctaccagagc
    14641 gtacagttaa aatagcatat aatacagtaa atatggcagc taaaacatat gatatattta
    14701 tgtttaagtt aaatccaatt tgagcattaa atatataact gaatataaca aaagaataaa
    14761 acataccagg aattaagctt ataatataat tcttttcttt tattataagg taaactgtaa
    14821 tcattgcaaa tgcaaatatg gcaatagttt gattactcca agagaagtat ctccacaata
    14881 tattaaatcc agaagcacta agctttgcaa atataagaat ggctatgaca ggaataaata
    14941 tacagattga caatataaca cgatgtttct tttccttttg gtcataatga aagtaatctg
    15001 caagcattaa tcttaaagat cttaaagctg tatctccaga agttattggt aaaacaatta
    15061 cacctattat agctatgata ccaccaatag aaccaagtaa atctcttgca accaaaccta
    15121 ttacatcagg agaacctact aattccttag gtattccttt attgtaaata cccatagcag
    15181 ctgcagccca aatcattgca attaaacctt ctaaaatcat catattataa aaagtagttt
    15241 ttccttcttt ttcatttgaa actgatcttg ctataagagt agcttgagtt gagtggaatc
    15301 cagaaacgat accacaagca actgtaacaa agaatgtagg aataagagga ataccatcag
    15361 gatgaatacc tttccaattt gcaagactta aattagctaa atcataacct tgtgtaaaga
    15421 ttccaattcc aacaccagca gcagataaaa gtagaatagc tccaaatatt ggatatacct
    15481 ttcctatgat tttatcaatt ggaaagagtg ttgcacacaa gtaatataga aagataagtc
    15541 cataaactat ccagacaact ggattattta tagtcgattt catatttaaa atttgagtaa
    15601 caactaaatc cccaggtgta taaataaaaa ctgcaccaac taaaagcatt aaaagacaaa
    15661 ggaatatatt atagacttga aatactttat tacctaaaaa tctactgata agagaaggca
    15721 tttgtgctcc tttttctctt atactaatca ttccactcat atagtcatgc aaagctcctc
    15781 caaatacaca gccaataggt attagtatga atgctattgg accaaacaat ataccttgga
    15841 taggtcctag aattggtcct gtaccagcta tattaagcaa ctcaattaga ctatttttcc
    15901 attttttcat tgggacatag tctactccat cactttgagc aagtgcaggt gtttttctgt
    15961 catctgggcc aaatactttt tcacaataag caccgtataa agcacctcct attagtaata
    16021 tagccaagcc gattaagaat gttgtcataa tatcacccca tttataaaat tttttggtct
    16081 ttgtatacat attatctaaa tgtgtagatt tatattgaat ttaattctgg acaacctaca
    16141 aattctataa agtattacaa ctaatataat ttcagaaata aatacatata cataaccatg
    16201 tacttagtat acaggtattt ggtaatattt tcaatgttat agcaataaag aatataaaca
    16261 aaattctgat aatattttac aaaaaaatta aaaaatataa aaattagttt aaatagatgg
    16321 agaaaaaagg aaaatcaatt gagaatataa atgtctatat cttaacaata ttaagaggtt
    16381 taaaattctt gaagaaagaa tagtcaagtg atataatggg tttacaggtg tatatacact
    16441 agtacatata gaaagcggtg caattatgga taaagattta acatatcaaa tcaaagagct
    16501 aaagaaagaa aaaaatgcaa taatacttgc ccatttttat caaccaccag aaatacaaga
    16561 attagcagat gcagttggtg actcatatta tttaagtgag attgctagag attgtaaaga
    16621 agaagtggtt gtattttgtg gagtaaggtt tatgggagaa agtgctaagc tattatcacc
    16681 tgaaaaaact gtattgatgc cagtaagtaa cgctgggtgt gctatggcag atatggtaga
    16741 tgaagaaggc gtgataaaat taaaacaaca gtatccaaat gctttggttg tatgttatat
    16801 aaactctaca gctaaagtta aggcacattg tgatgtttct gttacatctt ctagtgcaat
    16861 taaaatacta gaaaacatag ataataaaga aattatattc cttccagata aaaatttagg
    16921 aggatatatt gcagaacaat ttccagataa aaactttata ttttgggacg gatattgtaa
    16981 ataccataac aatataagag ctgaagaaat aattgaatta aaagataagt ataaaaatgc
    17041 agaagtatta gtacatccag aatgtaaaaa agaaataaga gatttaggag attatgtagg
    17101 tagtacatcc ggaataataa aatatgctac taatagtaaa aacaaagact ttataatagc
    17161 aactgaagaa ggtatattac atgaattaaa gaaaaataat cctaataaaa acttttatat
    17221 tcctggtgga aaaatactat gtacagatat gaaaaagaca acattagaaa atttatatag
    17281 tacacttaaa aatatggaaa atgaagttat tgtagaagat gaaatcatgg aaaaggcttt
    17341 aaattcactg cttaacatgc acaaattagc agaaggatag agggatagac ttatgaattt
    17401 agaacaagat gttttaatag ttggaagtgg agtttcagga ttatactgtg cattaaattt
    17461 agataaaagt ttaaatgtgc tagtagtatc gaaatcaaca attgaaaaca acaatacata
    17521 tcttgcacaa ggtggaattt caactgctag aaatattgat gatatagaat cctttgtaga
    17581 agatacaatg aaggctgggc agtataaaaa tagagttgaa gcagttcaag tacttgcaga
    17641 tgaatctata gaaaatgtag ggcagatagt agaatatggt atgcctttag acaaagaaaa
    17701 tggagagata gattacacaa gagaaggagc acatagtgta aatcgaatag ttcatagtaa
    17761 ggataatact ggagaagttg tttttaaaac tttattaaaa gaagctaaaa ctagagaaaa
    17821 tataactctt atagaagatg catatctttt agatatatta aaagatggca ataaatgtat
    17881 aggagctaga atttttaaat ctaaaaaaga gattcatgtt ttttcaaaaa tagtagtttt
    17941 agctacaggt ggaattggtg ggctttttaa aaattctact aatcaaagac atcttacagg
    18001 tgatggaatt gcaatagctt taagaaataa tataaaaata gaaaatttag aatatattca
    18061 aatacatcct acagcctttt atgaagaaaa taatgaaggt aggagaatgc ttatatctga
    18121 atcattaaga ggagagggag ctaaactttt aaataaaaat aaagaaagat ttgtggatga
    18181 acttcttcct agagatgtag tttcaaaagc tatttttgag caaatggaaa aagataaatt
    18241 accttatgtg tatttagatg caacacattt agatagtgaa tatttaatta atagattttc
    18301 ttttatttat aatgaatgtt tagctagagg aacagatata acgaaagaat gtataaaggt
    18361 atctcctgca caacattatt ttatgggcgg gatacatgta gatttggatt caaaaacatc
    18421 tatggaaaat ttatatgcag ttggtgaaat tagttgtaca ggtgttcatg gagccaatag
    18481 attggcaagt aattcattac ttgaagggct tgtattttca aaaagagcag ctaaaaatat
    18541 aaactcagtt atagataatg taaaagttaa gtttatagat gtaccagata tggatataga
    18601 tatagaacag gtaaaaaaag aaaataaaat attagtcata aaagaaattg aaagaacaag
    18661 tgaggatttt ggcgatgaat tatttgatta ttgatagaat gatacaggat gctttgatag
    18721 aagatgttcc aagtgaagat ataacaacta attctattgt ggatgaaaat tctaaatcta
    18781 ctgtagattt aatttgcaaa caagatggta taatagctgg cttaggtgta tttaagagag
    18841 tttttgagat acttggtgat gtagatgtaa aattatataa aaatgatggt gatagagtaa
    18901 agaatcgtga aaagatagct gtattaacag gtagtactag aaacttactt gtaggagagc
    18961 gtgtagcatt gaattattta cagagaatga gtggtatagc tactattaca agtaggtatg
    19021 ttgaaaagct tgaaggtaca aatacaaaat tattagatag tagaaaaacg atacctaatc
    19081 ttagaatatt agataagtat tctgttaaag taggtggagg atgcaatcat agatttaatt
    19141 tatctgatgg aatattgctt aaagacaacc atattggggc tgctggtagt gttaagaagg
    19201 cagttgaatt agctagaaaa aatacttctt ttgttagaaa gatagaagtg gaagtggaaa
    19261 ctttagacat ggtaaaagaa gcaatagaag ctaatgcaga tataataatg ttggataata
    19321 tgagtttgga aatggcaaaa caagcagtag atattataaa tggaagagcc attgtagaat
    19381 tttccggaaa tgtaaattta gatactatag aagatatagg aaaaattggt gtggatgttg
    19441 tttctgtagg agcgcttaca cattctgtta aagctctcga tataagtatg aagaatttga
    19501 gaaatatata agttttatat tgaaataaac ttaattaaaa tagactcctt aaatttgttt
    19561 tataaattta aggagtctat tttttattca ttaaaatt
//
LOCUS       DAEPOG010000065        18790 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEPOG010000065 DAEPOG010000000
VERSION     DAEPOG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.065"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(437..1216)
                     /locus_tag="KR432_001895"
     CDS             complement(437..1216)
                     /locus_tag="KR432_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH0640432.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            complement(1229..1963)
                     /locus_tag="KR432_001896"
     CDS             complement(1229..1963)
                     /locus_tag="KR432_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBH0640433.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            complement(1992..2195)
                     /locus_tag="KR432_001897"
     CDS             complement(1992..2195)
                     /locus_tag="KR432_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640434.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            complement(2198..2755)
                     /gene="frr"
                     /locus_tag="KR432_001898"
     CDS             complement(2198..2755)
                     /gene="frr"
                     /locus_tag="KR432_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH0640435.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            complement(2781..3485)
                     /locus_tag="KR432_001899"
     CDS             complement(2781..3485)
                     /locus_tag="KR432_001899"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH0640436.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            complement(3570..4481)
                     /locus_tag="KR432_001900"
     CDS             complement(3570..4481)
                     /locus_tag="KR432_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH0640437.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            complement(4560..5273)
                     /gene="rpsB"
                     /locus_tag="KR432_001901"
     CDS             complement(4560..5273)
                     /gene="rpsB"
                     /locus_tag="KR432_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH0640438.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            complement(5480..6664)
                     /locus_tag="KR432_001902"
     CDS             complement(5480..6664)
                     /locus_tag="KR432_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0640439.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            complement(6768..7826)
                     /locus_tag="KR432_001903"
     CDS             complement(6768..7826)
                     /locus_tag="KR432_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBH0640440.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            complement(8040..8684)
                     /locus_tag="KR432_001904"
     CDS             complement(8040..8684)
                     /locus_tag="KR432_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640441.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            8869..9492
                     /gene="ytaF"
                     /locus_tag="KR432_001905"
     CDS             8869..9492
                     /gene="ytaF"
                     /locus_tag="KR432_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBH0640442.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            complement(9555..11465)
                     /locus_tag="KR432_001906"
     CDS             complement(9555..11465)
                     /locus_tag="KR432_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0640443.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(12002..13069)
                     /locus_tag="KR432_001907"
     CDS             complement(12002..13069)
                     /locus_tag="KR432_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH0640444.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(13315..14310)
                     /locus_tag="KR432_001908"
     CDS             complement(13315..14310)
                     /locus_tag="KR432_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBH0640445.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            complement(14569..15828)
                     /locus_tag="KR432_001909"
     CDS             complement(14569..15828)
                     /locus_tag="KR432_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0640446.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(16067..16696)
                     /locus_tag="KR432_001910"
     CDS             complement(16067..16696)
                     /locus_tag="KR432_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH0640447.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(16889..17050)
                     /locus_tag="KR432_001911"
     CDS             complement(16889..17050)
                     /locus_tag="KR432_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640448.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(17393..18553)
                     /locus_tag="KR432_001912"
     CDS             complement(17393..18553)
                     /locus_tag="KR432_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0640449.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         5942 a         3242 c         1865 g         7741 t
ORIGIN      
        1 ttttatcata ttatgtaaat atattccttt tttttggtat attacttaaa ttatatctat
       61 ttttgtgcaa taagaccaat aatttactta tttcttatta attatattta aaaataaaat
      121 ttataaatgg ataataaatt agttacattt atacatatat attataaata tataataaag
      181 ctaaaaatat cgaaattatt aaacaaaaaa atatttttta atgatagtta tagtataaaa
      241 aatatactca ctacaaatat agtatactat tataaagtaa tacttattct tataaaattc
      301 cccaaggtct tctaaatttt tgaacaatga aaatagccat gtaactaata actgcagaat
      361 atctctgcat ttaaaagtta catagctatt ttcattacta gcaattatag attgctcttt
      421 taaatccaac caatctttat ctaataaaaa atttaatcgc actatataca aaaggtgcaa
      481 ctaatataac actatcaaat ctatctaata tacccccatg accaggtatt atctttccat
      541 aatcctttat ccctacatat ctctttatag aagatgcaaa taaatctcct aattgagcta
      601 ttacacttcc tatacttcct attatcgcta attgaagcaa atcaatgcca aataaatatc
      661 caaataaaat acaacaaagt gtacttccta ttattcctcc aatagctcct tctatagtct
      721 ttttaggact aactttagga attagcttat gttttccaag taaataacca ctaaaatatg
      781 caaatatatc tgtcataaaa gatatcacaa atataagcca tacatatata ctaccttttt
      841 caaatccatt tatagttata attataaaat ctaaaaatac tcctatataa aaaactccta
      901 aaaacgtaat cgaaacatct ataacattgt ttttgcccat caacatataa attatacttg
      961 ctaaaaataa aataaaaatt actgcataag tatattctaa tggtaaatta aaaatatttt
     1021 ttacagctaa ataaatagaa aataaatatc ctataatgaa tattggatgt acatctttta
     1081 ttttaaatgc cttataaaac tcatgcaatg caatataaac tattgctgtc tcagctatat
     1141 atagaggaat acctccatat acaacaaata gaaataatgg aaccagagct agtgaagcaa
     1201 taattcttgt aagcataaaa taatctcctt acttaagtcc tccaaatctt ctatcccttc
     1261 tttgatatac gtatatagcc ttctgtagtt cttctttttt aaaatcaggc caatgtatat
     1321 cagtaaagta aaactcagaa taagcaactt cccaaagtaa aaagttactc aatctttgtt
     1381 caccacttgt tcttattacc aagtctggat ctggtataga ctttgtgctt aaatagtttt
     1441 ttataatatc ttcatcaata ttgtttatat ctattttacc actttcacaa ttttttacta
     1501 tatctataag agcatttttt atatctgctc tacttccgta gtttaatgct aaatttaagt
     1561 taactcctgt attgtttttt gtcagttcct tagctgagtt taattctctt atacaagttt
     1621 ttggtaaagc acttatatct cctatagcag ttacttttac attgttttgg tgaagttcag
     1681 ctatttcatt tcttaagtat gtagataata agttcataag tgtgtcaact tcaagtttcg
     1741 gtcttttcca gttttctgtt gaaaatgcat ataatgttaa gtgcttaaca cctaattttg
     1801 agcattcttt tactatatct cttatagttt ctactccagc cttatgtcct gcagttcttg
     1861 gaagaaatct agcttttgcc catcttccat ttccatccat tataatagca atatgagtag
     1921 gtatgttatt taagtctata tcatatataa tgttgttatt catattttgc ctccaatatt
     1981 gttctgactc cttaaactaa ggagtcagaa aagtatgtga ttatcagttc tacactatca
     2041 cctatctcag atatttttat aactctagga actacaagtt tttctttatc ctttttacct
     2101 tcaatagatt ttacggtata atttatattt tgctcatcaa aatattgttt tacctcatct
     2161 acatgaagac ctaaaaggtt ataatatttt ttcaatatta tacctccatt atgtcctttt
     2221 cttttttaga taataatgta tctatttctt ttataaattt atcagtcatt ttttgaactt
     2281 catcttgtgc tttttttaat tcatcttcag ttaattctcc gcctttttcc atctttttaa
     2341 ttttctcatt ggcgtctctt ctttcatttc ttattctaac tttaaattct tctgcagctt
     2401 tactagcttt tttagctaat tcttttcttc tttcttctgt taaagcaggt acagaaagtc
     2461 ttataacctc accatcgttt gatggattta atcctaagtc tgagttagct atcgcttttt
     2521 ctatttcatg catagcagat ttatcccaag gagatatcat taatattctt ggttctggta
     2581 cagatatagc acctatttga tttattggag ttggagttcc ataataatca actcttattt
     2641 tgtctagcat ttgtgcatta gctcttcctg ctcttatagt tccaaattca aactttaaag
     2701 catcaattgt cccattcatt ttctcttcta attgcttatg tatttccagt ttcataattt
     2761 tactcctcct tcattcttca ttattttaca gtagttccta tattttcgcc ttttactgct
     2821 cttattatat tctctgttgt aagctcaaat acctttattg gaattttatt atccatacat
     2881 aaagatgtag ctgtagaatc cattactttt aattctttct gtataacttc catgtaactt
     2941 aattctgtaa atttcttagc atctgcatgt acttttggat ccttgtcata tactgcatca
     3001 acatttttag ctagcaatat aacttctgct tccatctcag cagcacgtaa agcagcagtt
     3061 gtatctgttg taaagtaagg gtttcctgtt cctgcaccaa atataacaac tctttccttt
     3121 tctaagtgtc taactgctct tcttcttata tatggttctg caatttgtct catgtctata
     3181 gcagtttgaa ctcttgtagc aacatctata ttttctagtg catcctgtag cgccattgca
     3241 ttcataactg tagctaacat tcctatatag tcagcagtag ttctatccat tccttcactg
     3301 gttctgcctc tccagaagtt tcctccacct actactacag ctacttcaac acctatttct
     3361 tgtatttttt ttatagcaat agcaatatca ttaactacat cattatttat accaaaacct
     3421 ctttctcctg ctaatgcttc tccacttaat tttaacaata cccttttgta cattggttta
     3481 tccatcagaa acctcctatt tcaatccttc atatccaatt ttttctattt tttaaaaaag
     3541 agaacacctg cgtgttctct ctttggttat tacttaagtt gcttagcaac ttcttctgca
     3601 aagttttctt ctctcttttg tataccttca ccaacttcga atctcacaac tttaacaact
     3661 tttatatcag aacctactgc cttagcaaca tcagcaacta attgtttaac agttatatct
     3721 ggatttttaa cgaaagtttg ctctaataaa caaacctctt ttaattcttt ttctaatctt
     3781 cctttaacca ttttttcaac tatattagct ggttttcctt catttaaagc ttgttgagtt
     3841 aatacttctg tttcatgtgc tatgtattca gcatctactt catctcttga aacatactta
     3901 ggattcatag cagcaacttg catagctaca tctttaccta attctaaaac tttagcatct
     3961 ctagcttctg tttccatttc aacaagaaca cctatttttc caccaccgtg aatgtatcca
     4021 gcaacttgtc cattagttac aactttagca aatcttctaa agtctaattt ctcacctatt
     4081 tttgcaactc tattatttaa aacttcttgt aaagttattc cctctttatg agtttcacct
     4141 aataaagaag ctatatcttc tttatcagta gctaatgcca tacaagcagc atctttaacg
     4201 aacactttga agtcttcgtt tttagcaacg aaatctgtct ctgagttaac ttctaccata
     4261 gaagcaactg tattatcatc attcatttct attgcaacta gaccttcagc agcaactcta
     4321 ccagcttttt tagcagcttt tgataatcct ttttctctta ataaatctac tgctttttcc
     4381 atgtttcctt cagcttcttg taaagctttc ttgcagtcca tcattcctgc gcctgtactt
     4441 tctcttaact cttttaccat ttgagcagtt atattagcca ttttttaagt ttcctcctac
     4501 ttattctatt ccatattata aatggtaagg gcgaccctta ccatttaatt gtcaaattat
     4561 tattcttggt cttcagctac ttcttcctca gcaccttgtc taccttcaat tacagctgtt
     4621 gccatagcac cagttattaa ttttacggct cttatagcgt catcatttcc tggtatagca
     4681 aagtctaatt gttctgggtc acagttagta tcaactatac caactactgg tatacctaat
     4741 ctatgagctt cttgaatagc tatgttttct tttcttgggt caactacgaa cattgcacct
     4801 ggtaattcag gcatgtcttt tattccacct aagtattttt ctagtttttc tttttcagct
     4861 cttaatttta taacttcttt tttaggaaga acattaaata cgccttcttc ttccattttt
     4921 tctaattctc ttaatttatt tattcttgtt tttatagttt tgtggtttgt taacattcca
     4981 cctaaccatc tttcattaac aaagtacatt ccacatcttt cagcttcatc ttttatcgca
     5041 tcttgagctt gttttttagt tcctacgaat aagattggct ttcctgtttc agctacttct
     5101 ttagtgaact tgtaagcttc ttcaactttt ttaactgttt tttgtaagtc gattatatat
     5161 attccatttc tctctgtgaa tatatactta gccattttag ggttccatct tcttgtttgg
     5221 tgaccaaagt gaacacctgc ttctaataat tgtttcattg atattactga catatcttac
     5281 ctcctggttt tatacctcca gcttgatctt ctccctaaaa aaccatgtaa taggcacctt
     5341 ttaaggatcc caatgctgtg tgtgatttat tttatttttt cacctctagt agtatatcac
     5401 agaggcgaag aaataacaat attttttcca tattttctat ttttatattt ttttgtgtat
     5461 tttataccat tttttgaatt taatttgcaa atttcatttt taattttgtt tttaatgata
     5521 cgttatctat actattatta cttactatat ctacacttcc aagtaagtca cctgaactag
     5581 tataatattt tataacaccc atagtatcac cttttttaac agggtactct atatttttaa
     5641 tcttaacttc tgattttata ctttcatcat cttttaatat tgtataaagt tcatcttttg
     5701 cttttaatgt aacctctaac tctttcattc cttttatata tttcttacca ataaagctat
     5761 ctttatcaat tacttttttc atattgaaat tttcttttcc atatttcagt agagctaatg
     5821 aagcagaggt tcttttattt ttatggttag tacctaatac aacccctatt actctacgtt
     5881 ttttctcatt tttctcattt ttatttacaa ccatagaaaa tgataagcaa tatccagcat
     5941 tgccagtata tccagttttt ataccatcta cttctggtat aattcttaat aatgcatttg
     6001 tattgctttt ttcaaaactt ctatctgaat ttgtatatgt ctccatatca gttatagctg
     6061 ttacttgttt ttcataatgg tcaaacatgt attttccaag aatagctata tcttttgcag
     6121 tagagatatt ttcttttgct ggttttttgg ggttgcttaa atcgtatatt ggaagaccat
     6181 ttggatttag aaaatgtgta ttactcattc ctatttcttt tgctttttta ttcattctat
     6241 ctacaaatgc ttctttcgtt ttacaaatat gttttgctat agcaattgcc gcatcatttc
     6301 ctgatacaat cataagacct ctcataagct ctataagtgg cacttcttca ccatctttaa
     6361 gtttataact tgaccctttt actgatgcaa tagatttgtc tatttttacc ttatcagttt
     6421 cttttacttc tttattttca atagcttcta ttgcaagtaa aaaagtcatc atcttactta
     6481 aactagctaa tggtaccttc atatcaggtt ctttactata taaaactcta cctgtttctt
     6541 ggtctataag tatagatgtt tttgaatact tgtctacaga tttaccatct gcatatgaat
     6601 aatttggtat tataaaaact agaatcatgg ctaatgatat aatttttttt agtgcatttt
     6661 tcaaattttt caccttcttg gattattaag tatatttata tttaaatttt ccctatttgg
     6721 tccaatttat atctagtaat taatacctat ccatatttac acaaatatta ccttatcatt
     6781 cttttaataa tcacagatgc ccaaattgct cctattgtaa ctccaaaaaa tacttctata
     6841 cctgccaatg ctttcccaat caaacttgta gcaaccatat ctccatatcc tactgtacta
     6901 aatgtagcca tgctaaaata ccataaatcc atatacattt tgaaaatctc acttagtgag
     6961 taatcaccta tattgtttat atctaaagct acaagtgtgc tatttgaact ttctatgcct
     7021 acaaataagt ataataaagc aaaaacactt attattgcta taatcactaa taatatattc
     7081 catggctttt ctccataacc tgttgtgtaa cgtgctattt ggtcaaaaaa tttttcaact
     7141 aaataatcac tgaaagtagg ataattagaa cgtttcatta ttttactact ataaattctt
     7201 tccttataat agtaaaatcc tatcatctca tattctccaa tttttgttgc ttgatttcta
     7261 gctattctat aatttatctt gccatgctca tactcacttt ttccatattc aacattcttt
     7321 aacacaaatt ttgtacacat gctaaaaatt attccatcca ttcgtggaaa attttctact
     7381 ttattaacta ccaaatcagg tcctttaaat ttcacttttt caaatatacg gtcatttata
     7441 tagtttttac taaatatggt tctattaaaa tcacagtttt ctaagaatat acaaccatta
     7501 aataaaactt tatctctaaa ttctgtatag ttaaaaataa tattcttttt aaaaatatat
     7561 tttttaaaat ttgctttttg tttaaatatg gattcagaaa aatccagtat tttataattg
     7621 taagttaata tttcttccgc attaaattca ttttcaaaaa caaatccatt aaattcactt
     7681 atctcttctt tatgaatagt atccatcatc aactgtatct cttcatcaga cttattttct
     7741 ttatgaaaga tacaatatcc agaagaatct gcttctctat gacatttttt tctctcatct
     7801 atcttagata tccatttaca attcataaat atatatcctc cagtacaaaa caattattac
     7861 tattttgtat tagtttggat attattttat tttatatact taaatagtta caaaatatat
     7921 attagattct tagttttaaa tttttaatct acttcattgt gcttttaatt tgtttcatat
     7981 aaaaaataat aaattttata tactttgatt tatttatata ttaaatatta ttctaaaact
     8041 tacttatcat ttttaagtaa ttttgtaact atatttaatg ctactaaatc atccataaat
     8101 cctattggga acacgaagtc tggaatcaca tctgttgata taacaaagta aagtagcata
     8161 cttccaatca ataaatattt ctttattgta agtccatctg tacaaaaatc agcatacatt
     8221 tgtcttaatc tatctgtaaa ttgaccttgt ttttcaatag aatctatttt tttattaaaa
     8281 ttatcaaaaa gtaatttttt accttcatct gtttggacat ataacttaca cttacttaac
     8341 tctttttcaa tatttccatt aaagttctta tcattgatta aatttgcaaa tccaagaata
     8401 tcatcaaagt tactaaaatc actatctgca ttaaatattt gattattgct gtttctaaat
     8461 tcatatcctg aagctttcaa cagttcttct aggttaatat ttaaatgtat tgccatcttt
     8521 tctaaatgat ttatatttgg cctttgttta ttattagcta tacgagatat agtagattta
     8581 tcaattccac tttttttact tagttctccc attgataatt ctttttcatc taataaaact
     8641 ttcaacaaat caccgaaatt cccaacctta ctatgttgtt ccattatttt cacctgcttt
     8701 attatgattg gttttatatt atatgatatg attcttttgt tatttaggtg ttgtatgatg
     8761 ttgaaatttt atcaacaatt tattggatta tctgtaacaa tttatccatt tttacaatac
     8821 tataactaat ataagtacaa aatttaagag aaaggatgtt aatctactgt gattgaatca
     8881 attttattag ttacttctct ttgtatagat gcctttgtga caagctttgg atatggagta
     8941 aataaagtta aagttccctt atattcaaat ataattatca gcgtagtttg ttcagctatt
     9001 ttagcaatat cgctttttgc aggtacaata gttagagatt ttttacctca aaatataaca
     9061 atgttaatat gtttctttat tttattttta ttaggaagtg ctagactaat tgaaagtttt
     9121 tttaaatctt attttaagaa aaaatctata cactcaaaga attataattt taatatgtta
     9181 gaccttgaat taaattttaa tgtaagtatc gaacctgatg tcttagatac aagtaaatct
     9241 aaagtgctta aatctgctga agcatttaca cttgcacttg ctttaagttt agatggatta
     9301 gctatcggtt ttggtagtgg ccttgtaggt gtgaattata tacaagttat tctattttct
     9361 ttaatatcaa atataattgc agtattactt ggttgtttac taggaaatat gtttgttaaa
     9421 aacgtaaatt taaacttatc ttggttaagt ggtttaatct tgttagtcat tggatttatg
     9481 aagatatgtt aataagaaag caatctaaag ataaactttt ctctttagat tgctttcttt
     9541 tgtgcttttt atatttattt tctattctct ctaatttctt tctgaatctt tttaagagct
     9601 ttttttgaac ctctttgaat atctttttct aattttcttt ctttaaaact agtaaataat
     9661 acatctaaat catacccttc tggatacaat tctgatgctt ttatgctaag ttgtaatctt
     9721 tttatattta ctgaaataaa attatcatta aacaatactt ctacattatt aaacttgtcc
     9781 ataggcttat atacaattcc aaaatcatct tcatctagta attttacctt atcccctaca
     9841 ttataatctg tatagcttaa aatgacttca gaactatttt tatcttcaat attagattgt
     9901 agcttacttc ttttaactaa atctaaattg tagtttctat caattacata ttctttagct
     9961 ctatctaaaa ctctattttt tattcccatt tttttagaaa taaaaagagc attactatct
    10021 tcagattttc caatagttaa cttatacaat ggttctaacg tttctttatc aaacatcata
    10081 ccagcatttt caaattctgg atgtaatgta gcaaactttt ttatctctcc ataatgagtt
    10141 gatgctatag ttatacatcc cattttgtaa aactcttcta ataatgatat tgctaaacca
    10201 gctccttcat taggttcagt tccacttcct atttcatcaa acagaacaag agtagaatta
    10261 tttgataaac tcataatctc tgcaatattt tttatatgtg atgaaaaagt acttaaggca
    10321 ttttcaatac tttgattatc ccctatatct acaaaaactt tttcaaatac actaaattca
    10381 ctaccttctt ttgcagaaat atcaaatcca cattgaacca ttaaagttag taaaccaaca
    10441 gtcttaaggg ttacagtttt tcctcctgca ttaggacctg ttattataag acttctataa
    10501 tttttgccta tttcaaaatc taaaggtact gcatctcctg ttagaagtgg atgtttacct
    10561 ttaattattt ttatatagcc attattattt atttttggag taattccttt tattttttga
    10621 ctatacttag cttttgcaaa taccatatcg tattctgaaa gtatctctat atttaatttt
    10681 atctgactta tcttctcaaa tataagttct gttaaataag ataatatttt gtattcttca
    10741 atagtttcat cagcttttag catagttaac tctgtactta aatttgaaac actaataggt
    10801 tcaataaata cagtatttcc ttttgatgat gtatctaata tagtaccatt aacttcattt
    10861 ttataagaag atttaattgg tattacatat ctatcatttc ttttacttat tataaactct
    10921 tgtatatact ttttatttac agttgatgtt ataaatttat ttaatctatc ttttatcttt
    10981 ccttctgtaa tttctatatt tcgtcttatc ttttttaatt ctttactagc attagaatca
    11041 actttattag attttataca gtaatttatc tcatcttcta tacttttaca ttctgttata
    11101 tttaaagcat aagaacttaa tgtaggtgaa taaaattctt tttctaacat aaaagctttc
    11161 atcttcctac aacctcttaa aaaatcttca atatttacaa gttcaacagg ttctattatc
    11221 attccttttt caattttatc tattgtagaa cctgcattaa acaatccttc aagtggtatg
    11281 tgattactat tttcaattat ctttctagct tctttatttt ctgctaattt ccttcttact
    11341 actccaatat tcccactagg agttaattta tctattaaat tttttcctag cgaacttaca
    11401 caatgtattt ttattaagtc tttaacttca tttagttgca atctttcaaa tgtattacta
    11461 ttcattattt tttctccttt atttataagt catcgtcttt taaatcaccc tatcatttac
    11521 tttgataata gttattttgc gaacaactta ataaataagg agttattata tatcaaactc
    11581 cttatttatt aagaagttta actccggact cattaatgtt agtatttttt ttcataaaac
    11641 acttctcctt ttctattctt atttaaacac ttataccagt gcttctaata ttattccata
    11701 aattatatat ttatgtcaat aaaaataatg ttattaatgt ttttgtacag tgaaaatttt
    11761 tatagtgaaa ataagcttat actttcattt atattttgga attacttata ttgtcatcaa
    11821 ttcatgtatg tgtagtttcc aatacaatct aactttgtaa tcattatata tcaaagaatt
    11881 tagctctata aattatcgtc tctataaaat aaaaaaatag ctaataataa taaaacactt
    11941 ttaaatgttc cataaaatta gctatttata atataatttt aatctttttt aactttaaaa
    12001 attatccttt ataagtaatc aatggcttca tagttgccac tacttgaata agttcatatt
    12061 ctatcaaact ttcaataatc tgctctatat tcttatatgc ctctggagct tcttgaaata
    12121 ataaatttac atcattacat ataatctgac ttttaaattt agtttgtttt atagtatctt
    12181 tactatactt acttttaagg cgcgatttac aaactgaacg agaccatttt ctacctgctc
    12241 catgtgatag agaatataaa gatatctctg tattttcagt aggcataact atatatgata
    12301 gactgcctct tgaacctggt ataatcacag ctcctctttc acttgaaaca gcaccttttc
    12361 tatgtataaa tttatcttcc cttttctcta taaagttatg atttatagag aatattgttt
    12421 caacatcttc agatgtacct attgactgca taattttttt actgatagct tcacgattac
    12481 gctctgccca cattaaagca ttatcatgtt ttttaatata atctattgct ttctcacttt
    12541 caacttcaag tccatcttta tcataaaatt tttttaaaat atcttctcca taatttcttg
    12601 acccacagtg aactaacatc ataatatcat cagatgaaaa ttttaattgt tcaaattgtt
    12661 ctttattata aatttcactt atacattgaa tttctacaaa atgatttcca ctccctatag
    12721 ttcctaaatt taaaattgga cattcttctt tatatgtgtt ttttatttct atatcggaca
    12781 aatctttaat cttactaagt gacttaatcc atttgtcttt tttaaatttc ttttttagaa
    12841 ccccagtttt aaataaagtc attccgcatc ctatatcatt tccaattata tgtggataaa
    12901 taatattttt agtctcaact gctaaaccta caggtatttt acctgtatgt aagtcaggta
    12961 atcctactac tcttaaaatt ccatttaact ttgaaatatc ttctagttgt ttccttgcta
    13021 tatcttcaat ccaacattta ttacttgcta ttatttttat attgctcata atttgctcct
    13081 ttttaatatt ctcctgcctt taaaaagtag caaaaagctg tgacctttag ccacagcttt
    13141 tttattctaa actgtaaaag caggtggcta tttgttaaaa tactcctaga cgtttaatac
    13201 ctcattattt tttgttaaca taataaccac ttcctttcat aatttaagac tacactaaaa
    13261 tttctatgta tatcatttat agacattgat tttaaaatac ttaatataca atttttatat
    13321 tatggaacta ataataatca ttgaaatcat gactatagct agaccggcaa caacaagttt
    13381 tttatgtaat ttttttctca ttttactttt atattctttt ttaacttgta taactttgtc
    13441 ttgtttctgt tcttcttctt tctcatcttc aacttctata tatgcttttt catacattac
    13501 tttacttaaa tcaataataa tatcattggc tctagtatac ttattatttc ttccaagaag
    13561 cctttttata acatttataa gttctgtatt tccattaatt gcttttatac tattccaatc
    13621 taatccctta tcaatagatt gtagcatttt ttcttctgaa tttgattctc caaaaggcaa
    13681 ttttttaaat atagattcaa atagaataag acctgttgca aaaaaatcac tttctttatc
    13741 agtataattt atacatagtt gatgtgggca taaatatctc ctgtttccat agcttcttgt
    13801 attaacccca ttatttgctt ttgtaattcc aaaatcacaa atttttatat tgtattctgt
    13861 atctactatt atactactcg attttaaact accatgatat gaatgattcc tatgtatcat
    13921 ttctaaagct tttaaaactt gtatcattat gtttacaatt gcttctaaat gaagataatt
    13981 acctagaatt aattcatcta gtccgatacc attaaagtct tcacttacaa cataatataa
    14041 tgttgtttct tctgtacagt gaatcccaac atctattatt tttagtatat aaggagagtt
    14101 tatctcatct aaagcagtac tttcatcaat taaatttgaa acaaaatctt cacaaatata
    14161 attattgtgc tttataactt ttattaacac atttctcctg taaaaaacat ctctagcttt
    14221 atagattttg ttaatttcta aaacgtcatc agaatttact acctcatatc tattgcctat
    14281 aaaattcaac catgtcacct cctcatacat tttatataac taaatctatt tacaattata
    14341 ccaatatata ggaatatttt ctatatatta atgattgtaa tattaaaaca agaactaata
    14401 gtgttaattt ttacaaaaaa ggctgtctca aaatagagat acgcttcttt tgtattgttg
    14461 aaatgatgaa aatatatttg atttcacata aaaagagtgt ctcataaact aaaaagttca
    14521 ttttgagaca ctccctttta tattacaaat atttctctaa aaaattatct atttttttaa
    14581 ttctttataa gaactatttg atatatgttc tttttttatg acctttccac ttgaatcttt
    14641 aaatattcga tatgtagaat atttattctg accagtttgt tttacctcta tagatatatt
    14701 tggtttgtca ccagaattac catagattac tgatgataca cttcctccac ctgcataatt
    14761 tttaatatat acaggatgct tatatggatt tttaaacttt aagtttaatc ctccatcgct
    14821 cacagtagca tctcttcctt taggcacata agtagatgca gatgaatgat ttgtaacttg
    14881 agttatctcc atacccgaaa gaagagccgc attataaact gttgaagatg tttgacaaac
    14941 acctcctcct gctgattgtt ctagcttacc ataaactata acaggtgcat ctttgtaacc
    15001 atttgcttta ttactaggtc ctgtaagttt attgtatgaa aattcttcac ctggcattaa
    15061 taatacatca ctagtttttc ttgctgataa tcctacatta tatgacctac cctctacagc
    15121 ttgtgaataa gtagttgaat attgacctat aacagaattt acagttttta cttgttcagt
    15181 tgttatatct ggtttttgaa gatttacttt aagttctaaa gttttatgct ctttattttt
    15241 tatcatatta tatatagact ctttatttgc tgccaaatca aattctctac ctacaacaga
    15301 gtctgtataa cttagtccac cacttccaac actaatagaa gcatttttca tcttaacatt
    15361 tatactatct gctattgaag acactttgga actaagtttt gcctcatcat attcagcttt
    15421 tatcttcata tttgccttat tcactcttat atcaatatat ttttttaagt tttgaaaata
    15481 ggaccctttt tttgtacttt cataagcatc ttttacaaca tcttctgtat catattttaa
    15541 atcaatatct tttggtgata ttgtatattt tttcccatca taagtaagat ttaagtcttc
    15601 tggagtatac ttatctgtta tagcttttaa tgcctctgct ttagtcatat cagatacatt
    15661 tactccttct atatatacat ttttagcgat tttcccattg tacagaaatt tactgttaaa
    15721 aaaataagta aatccaccca ttattagaac taaagctgcc aatacaatta aaacacttcg
    15781 ttttttttct ttacctaatc ttgaaactct tgttgttctt ctgcccataa cttcacctca
    15841 ttttgacact agcttaaatt tgcataatag agttcccatt tcaaaactac cttagattat
    15901 atttgaatag cttttgttaa acaaggaatt taatcaaatg taactttatt gtaaatttat
    15961 gtcttataat tagttttatt tttgatattt tttattatat ttcttttact atttcttaac
    16021 aaaaaattgt ttattttata acaactaatt tcatttatat tgaaatttaa atgtaaccta
    16081 ttacaggata atggtctgac aggtaaattt tttctactgt gtatcctttt aattcagtat
    16141 ttttattaac aaaaatgtaa tctattctag agtttgattt ttcaaatgtt ggtaagtaag
    16201 acttgtttag tgaaatagcc atatcattaa acatacttaa aaagacattt ttctcattaa
    16261 aatcaccaca taaaataact atccccacta atctgttcct atagtctatt atttcgtcta
    16321 attgttttgc tctctcctgc ctatcaagtc ccaagtgagt atttattaca tttataattc
    16381 ttccatatgc atctatagtt atacaaagag ctccccgttg ttcttttttg ctagttaaga
    16441 aaaaatgatt actgtttaac attttagatg ttgtaaatgt acaaatacca tatatcatat
    16501 ttactttttt tacatttgta gcaaagacac catccatatt taaaacagct tttaaagcta
    16561 aaaattgagg atataatact tcttgcaaac aaattacatc acaatccaat tgttttaaat
    16621 ataaccccat tttagttaga gttaatctat tatttgaatc cataccttta tgaatattgt
    16681 aggttactat tttcataata atatcacctc ttatgcaata taatacttac taaaatttaa
    16741 acttatttac ttttcactta agaccattat ttatcttata aaattgagta cttataaact
    16801 ttatcacaaa aaaacctaac ttaagtattt atattctaca taagttaggt tttataaact
    16861 attataatat ttattatttc attcatttct aatgatgatt ctcctttttt atctttttca
    16921 atttgccttc actaacaaca aataaaaatc ccatcttctt gtatatttca tccaattcaa
    16981 ttaaagttac ttcaccattt tttaaatttg ttaatgttat atcttgtatt cttcttgatg
    17041 gaattatcat aagttatcaa cactccttta aattcttttt aatctttata tttaaatata
    17101 tttatgcatt ctgatatata ttcataaatt attaaaataa attcaaaaaa cacccgttct
    17161 gcaaacattc gttctgtata tttattatac atcatacaga cgttcgattc aatataaaaa
    17221 ttaaatatat ctcgttttta gtaaatttta caatattata cactaaatat gtcaaaacat
    17281 cttcttgaaa tagattatct tgtcaaacaa aaaaaaccat ttcagtaaaa gcttctgctt
    17341 aaattgaaat gacttttctt atttaaatta ttaattttat accatatttt tattaagcta
    17401 acatatgtaa tatttgagtt accacaagag cacaataagt ccctataata tttcctagaa
    17461 gccccataag tacacctact ggtattaata caggattgtg aaccccagca atcattggag
    17521 ctgtagttac tcctcctata ttagctatac ttcctacttc acaagtaaat aaatcaagtt
    17581 taaatagttt agcaagccct ataaatgaaa tagcatgtac aaggactact accaatccaa
    17641 acattatgta aacaggtgct tctgacaatc caaagaaatt tgctttacta cctgtagctg
    17701 caaggagtaa atacataaac acatttccta ccagtgtact ccctttaagc tttgatattt
    17761 ttgtcatacc caataatatt gatactgtag atgcaacaag tactacccac atagtttcat
    17821 taagtatacc tgaagttgga agtatcttgg atacaatttg tgcaaaagac gcaactccaa
    17881 acccaacaga aagtaaaatc ataagttcta caaacgtagg tgccgtatta tctttatttt
    17941 ctttttcaag ttttgatgta acctcatcta taaaacttgt atcgcacttt gtccatttgt
    18001 taaatttagg tccaaatttc acagacatta gacaaacaga catccatact gtatatccta
    18061 tagaacccat aagtattgca taactaagtc cttcttctgc aactcctaga gcattagctg
    18121 ctgcaatcat atttgatgag ccacctatcc atgaaccaga tgctgcccca agaattttcc
    18181 atgaatctgc acccattgaa tttttaaaaa ttatgaatgc tataacaaat ccaagcataa
    18241 atgtaaatgt agcacagaaa aatgataata acattttagg ccccatcttt aatatttttc
    18301 ttaaatcaca ttgaattaac aagaacacta acattaatgg aaggaagttc actgttatta
    18361 tgttttgata cgttccaaca tcttcattct gtgcaaataa accaaatgat ccaagtatag
    18421 ctgaacctaa gtatattaaa agcattggat taataacatc aaataccttc catttcaatt
    18481 tatcaaccat aaaaaaaatt acacctacga atacaaccaa gaaactacaa tatacaaatc
    18541 cagtctcaat cacattatac acaccctttc ttttcctaat taatttgatt tttatgattt
    18601 ctaagtaaag actttttcag ataatagaca attaaaataa atacttttat ctatatcatg
    18661 ctaattaaat acatatatac ctaactattg acatatatta ataaagattt gcacattaat
    18721 tactttagaa atgaataatc tgaatttaaa atctttttca acaattattt tattactaaa
    18781 atttttattt
//
LOCUS       DAEPOG010000066        18629 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEPOG010000066 DAEPOG010000000
VERSION     DAEPOG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.066"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            30..905
                     /locus_tag="KR432_001913"
     CDS             30..905
                     /locus_tag="KR432_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBH0640450.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1266..2564
                     /locus_tag="KR432_001914"
     CDS             1266..2564
                     /locus_tag="KR432_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBH0640451.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2754..4145
                     /locus_tag="KR432_001915"
     CDS             2754..4145
                     /locus_tag="KR432_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH0640452.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4322..4909
                     /locus_tag="KR432_001916"
     CDS             4322..4909
                     /locus_tag="KR432_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0640453.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4942..5733
                     /locus_tag="KR432_001917"
     CDS             4942..5733
                     /locus_tag="KR432_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0640454.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5914..8331)
                     /locus_tag="KR432_001918"
     CDS             complement(5914..8331)
                     /locus_tag="KR432_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640455.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8344..9009)
                     /locus_tag="KR432_001919"
     CDS             complement(8344..9009)
                     /locus_tag="KR432_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640456.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9091..10122)
                     /locus_tag="KR432_001920"
     CDS             complement(9091..10122)
                     /locus_tag="KR432_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640457.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10112..10798)
                     /locus_tag="KR432_001921"
     CDS             complement(10112..10798)
                     /locus_tag="KR432_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640458.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10956..12020)
                     /locus_tag="KR432_001922"
     CDS             complement(10956..12020)
                     /locus_tag="KR432_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBH0640459.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12282..12953
                     /locus_tag="KR432_001923"
     CDS             12282..12953
                     /locus_tag="KR432_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640460.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            12955..13863
                     /locus_tag="KR432_001924"
     CDS             12955..13863
                     /locus_tag="KR432_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640461.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            13940..14626
                     /locus_tag="KR432_001925"
     CDS             13940..14626
                     /locus_tag="KR432_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640462.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14623..17178
                     /locus_tag="KR432_001926"
     CDS             14623..17178
                     /locus_tag="KR432_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0640463.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(17372..18571)
                     /locus_tag="KR432_001927"
     CDS             complement(17372..18571)
                     /locus_tag="KR432_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBH0640464.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
BASE COUNT         6916 a         2326 c         2736 g         6651 t
ORIGIN      
        1 gatgaaacta atggggaggt taaaactcaa tgattacttt tgatgtaaat ggagctatta
       61 tcagatttga tgatacaaaa aataattata ataagataag gaaaatcttt aaattatatg
      121 ctttagaaat atctaaaaac tttgagagag attgtttaaa tagttttcaa aatctaaagc
      181 aaatatctga tagaggttta agtttaggag aaaaatatat agaagagtct tttaagaaag
      241 gaattgagac tatagtaagt tttggaatta taactataga tatcgatacc tttaaaagcg
      301 tttattgtga aaaatattta gattttaaga gactttttaa taacttgaat aaaactttta
      361 ttataccaaa taaaaataaa aaaaatgggc acgctagctt cttagaaata gaatcgacaa
      421 taaagaaaat gagcaattat ttatacaatg attgttttaa aattcattat gctgttgtag
      481 atgcccttat agaaaatcgt ataactgcgg tacattctta tattgatgaa gaatctataa
      541 aaaaatctaa tgccttattt aataattata aagatggttt tattgggaag gctgacgaat
      601 gcaaagtagt aaaacaaata ataactttaa atccatatag ggaagatgta tacgaatttt
      661 tggtaaaaga agatggagat tttagtaagg aaattgagag attgactgaa tttttaggct
      721 atgatataaa agaatataaa gcgtcattaa tggatattta tataaatgag ttaatggaga
      781 gcgatgaaaa tgatgttgaa aatgcaaaag aaaagattga aaaatatgct agatatattg
      841 gatgtagtga tgagaattta tatgtagcaa gagttaatgc catatatact tttgcaaatg
      901 cataatttta ttaaacatta aatattagat gttcaattat ataaatttaa ataaaaaaat
      961 atccttatta ataacaaata gtttatatta aaaaccattc ttaaaatgag tggtttttta
     1021 ttttgaatag atatatttta aacctaatag cattcctatc tttaattagc caaaaatcaa
     1081 ctggatttca tatagactac ataattttaa attactaaaa aagacaatat attgacaaaa
     1141 atgaaatatg ttgataaaat gaaatagtca atatggtgtc ttaatttaaa aatttattta
     1201 tgttgtatta gataaagttg ttttatatat aaagcataaa taattattat aaggagaaat
     1261 agattatgaa agtaaatgta tacaatgtag aatcatttgt ggatgaaaga cttaagatta
     1321 tggtttcaga cttaattcca aatactaaac ttaaaataag tgtgaaaatg gaatttccat
     1381 ggtgcaaggg agaagaattt tcatcttatg gagtgttttg ttcaaatgaa aaaggagaag
     1441 tagatttaga tttagttgaa cctatagaag gtacttataa ggctgataat tgtatggggt
     1501 taatttattc gctggaaaaa tctaaaactg aaggtaaaaa ttttgcagaa aatatatcta
     1561 ttgataaacc aataattatg aatatgatat ttgaaacttc tactgaaaga aaagaagttg
     1621 aattaaagag aatatttaaa tcagaagata ttaccacaaa atacatatct gatgagttta
     1681 taggaaaatt attttataaa caaaattcta ataataaaac aattttaatg ctaggtggtt
     1741 ctgatggaaa tttagacgct ctagaccttt tagcagctcc acttgcatct agaggattca
     1801 atgtactaac agttgcatac tttgctttag aagggttacc agataaatta gaagaggttc
     1861 cattagaata ttttgaaaaa gtatttgagt ggattagtga aaatgaaatt accagtacta
     1921 aagaaatatt tgtacatggg acatcaaagg gtggagaatt ggccttttta cttgcttcaa
     1981 gatatgaaca aattaaaaag gttgtagtat cacaacctca catttattgt ttccaagctc
     2041 ttaatggatt gatgagtgga aatgatactt catcatggtc atataaagga gagccatttc
     2101 catatatcaa agtagacaat gatatattct ttgaagaaca gaaaaaaaat atatcaaaag
     2161 gtattccatt tggatttaat agtacttata agaaaagtct taaaagagca gagaatagaa
     2221 aagaggctag aataaaaata gaaaattcca aagcagatat acttatgatt gctggaaaag
     2281 aagataacat atggaatagt tatgaagctt gtttagaggc attagaaatc tttgaagaaa
     2341 ataattatcc atataacgta gaattattaa catacaataa tatgggtcat ccgttaccaa
     2401 ttccatatat tatgcctcta aaggagactt tatgtatgag tatgagtgga gggatttttt
     2461 cttcaggtgg aactgtagaa ggaaattcaa aaggacagtt tgaatcatgg aatagaacta
     2521 ttgaatttta taaaaaactt ttatcaagtg atttaaataa ctaatattaa gtgatttaag
     2581 taactaaaaa tgaagcggtt tttctatata tgcacaaaat ctatcaattt tatagattta
     2641 atttattgtt tatataattt gcatgttata tactaatttt aatgacattg acaatttata
     2701 aacaattata aagttgtaaa cttataagag ttattgaaag gtggagtgaa gttatggaaa
     2761 gactcgctag aagggaacaa cctcatataa aatttggaat gtttaagata agaattccat
     2821 ttatacatta cagatttgag aaaccagaag ctattcaagg tataatatca agtatgacaa
     2881 gtttaggcac aattggcctt agtacacaaa ttttgggatt accctatgaa gtggcatgga
     2941 gtatggctat tattaactca atattatact gtttgcatgt ttttatggga gacccagttg
     3001 tacctggctg gattacagct tctataactc ttacaactgc atatttgtta aagttcagca
     3061 tgggcattga aagaatacaa gcattaacag ctttacaaat agatttagga ataatattta
     3121 ttttaatggg tataactggt gtagctggaa agttagttag taagatacca aattctatta
     3181 aaggtggaat actaatgggt gtcggtatat cgactataat atcagagttt aatcctaaag
     3241 ggagatttga ttcttatcct atatctataa cagtaggaat tttggttgct tgttttgtaa
     3301 tgttttctga aagatttgat actctaaaag ttaagaataa atttttattt catttaggag
     3361 aatatggtgt agtatcagct attttagtga gtttaatagt aggtatattt agtaaagaaa
     3421 tagaaattcc tagatttagt tttgacaatt tagtttacat agtagatttt aaaaatctga
     3481 taaatacagt atctcctttt ggaataggtt ttccaagtgt aatgttattt atacaaggga
     3541 taccaatggc ttttatgata tacataattg cctttggtga ttttataaca ggtgaaaact
     3601 tagtactttc tgaaagtgaa aatagaaaag atgaatatat agatttcaat tcaaataggt
     3661 ctaatgttat aagtgggata agaaatattt ttatggcaat cttttctcca tatataccaa
     3721 tgtgcggtcc attagctgcg acacttacag gaagtgttgc tcaaaggtac aaggttggaa
     3781 aggaagccat gcagtctgtt tttagtggca tgggtacatt agtatgggtt tcagcaattt
     3841 ttatctgttt ttatccaatt gcgcagattg ctacacctct tataccatta gcattgtcag
     3901 taacactttt agttcaagga tatttatgta caaagctatc aatggagcta tgtaatacag
     3961 gtgttgagaa gggattgatt ggacttatgg gaggtgtaat tgctgctaaa ggcggaacat
     4021 ggggacttgc tgtaggtttt atactgtatt tcattttaat tgattctaaa aaaagaaaag
     4081 aaaaagatat taattcaata gaagattgtg tatatgaaat attagacaaa aagaagagta
     4141 tttaaaggaa aatatgagtt gctgttatct attaatagat taattgcaac tcatgtttta
     4201 tttataagtt ttaggaattg caaaaaaatg caaagctttt tcttgacata atttcttgat
     4261 atgttactat agttatataa catatgtgtt ttataaaaat ttaatggaga ggtgaacata
     4321 tatgccacct aaagtaaaga taacaaaaga aatgattttg aatacagtct tggatattac
     4381 aagagaagtg ggatttgaag ctgtaaatgc gaggagtatt gcaagtaaat tacagtgttc
     4441 cactcgacca atttttactt gttacgaaaa catgaatgaa ttaaaaaatg aatttcttgc
     4501 ttttgcatat gaatattata atcagtatgt tgttaattat cgtaattcta aaagtgtaag
     4561 cccttattta ctgcttccgc tttcatacat tgaatttgct caagaagaaa cgtatttatt
     4621 taagttgtta tttataaatg atttagattt gatgatgaaa gaggcgaatg atttttataa
     4681 agaaattgac aatgaaaaaa aggcaagact tttttcggaa agcattggaa tagagttgga
     4741 atgtgcaaaa gtaatatttt tagatttatt tctttatact catggcatag ctgtcttaac
     4801 agcaacaaaa aaattaacat tagatagata tagtgctgaa aaaatgttga aaaatatatt
     4861 aacagctttt ataagacaag aaaaaccaga ttggaatttg tctatttgat gtttattaac
     4921 taatatagaa gaggtgagat tatggaaaca aaacaatact taattgactt ttataatact
     4981 tatgatgaag atagtcgact tgcattaaaa catggaatgg tggaatttct cactactatg
     5041 cattacattg ataagtacat aaaatcaggt gattgtgtgc ttgaaattgg cgctgcaact
     5101 ggtcgttatt ctcacacact ggcacgtcag ggatatgatg ttgacgcagt ggaattagtc
     5161 gaacataata tagaggtttt ccataaaaat actcaatcaa atgaaaatat aagcattact
     5221 cagggtaatg ctatggattt atctgttttc ccagataata aatatgatat tacgttactg
     5281 ttaggaccac tatatcatct ttataacaaa gaagataaac aacaagcttt acatgaggca
     5341 attcgtgtaa caaaacctgg tggtgtggtt tttgctgcat acgttatatc tgatggctgt
     5401 ctcattgacg aggggttcca tagaggaaat attaatgtat ctgaatatat tgaaaaaggt
     5461 ttgattgatc cacagacatt tgctgctaag tctgaaccaa aggatttgtt tgagcttgta
     5521 cgtaaagaaa acattgatga tttgatgtct gcatttaatg taactagact gcattatgtc
     5581 gcatcagatg gtttggcttt gtatatgcgt gaagcagtag actctatgga tgatgatgca
     5641 tttgcgttat atttaaaata tcaccttgct acttgtgaac gtgaagattt agtaggagtt
     5701 acaagtcatg caattgacat atttagaaaa taaggaatat gcttgtttat gtctctggtg
     5761 atgttgcttg gagatataag gaagtgtatc tgtataaaat aaaaacaagt agagggacag
     5821 aaaattatgt aaagtcaaaa ttttctatcc ttttagcttt taaaaaatta tgttaaaact
     5881 tgtataaaaa atggttattt tttatataca ttcttactga gaacgtaagc gttctgtaag
     5941 tgattttttt tctatatttc ttataagtaa ttcagtcact aaaacttgta taccaattaa
     6001 tacgaatgaa aaaattaaaa tagtaaatat agaaaattta aaattaataa agtctaatct
     6061 aattacatct tttataaaag tgcaaagtag atttgaacca aaataaccta gtatacttga
     6121 agtacatata gctttgatac ttatgtttag accttctcta taaagcatat ttcgcagttg
     6181 ctttcttgtc attccaacgg cttgaagtaa ggcaaactct ttttttctaa ttaaaacgct
     6241 gtttattagg gtattggtca tatttagtat accaaagcaa gaaattaaac caacaaaaat
     6301 gtatgaagct ataattatat attgaaatgc tctagtaatc attctataat catcttcata
     6361 tgaataaagc attaaatttg aatttttctg aattatactt aacaactttt cttcaacttc
     6421 tttagaatgc tttttatcta ttaaaataga aaaatctaaa tttgtattat atggtgttag
     6481 agattctaaa gttttgtctg aagcaaaaaa catacctcct gtattggaat tgtcaatgat
     6541 tcctataatt ttcattttct ttttaataat atcatttcca ctaaagatat taaatgtaac
     6601 tgtatctcca acttttaaat ttaatccata agattttgtt ctatatttat ttataattaa
     6661 actatctgaa tctaaatgtg tatagtctat ttttccactg acaatatatt cttcaagtga
     6721 ttttgaaagt tcaggcgtaa tacctttaat tagagtaata aattgttttt cgtctctatc
     6781 tttgatgtaa ggttctataa tattactttt aattgatttg gattcaatta atttttttac
     6841 tccatgaata gatttaattt cattcttaag agaatctgtt agtggattat tttgttggat
     6901 ttctgtaata tgctcatata agttatcact cttaatgcta attatataat cttcgttata
     6961 gacaaattgt tgagatagtt gttttttgaa atctattgaa ttagcaagat tagcgattga
     7021 aataaataaa actccactaa tgcttagcga tattaatgca atcaaatttt tctttctatt
     7081 tgactcgata ttctttaatg ctagattttt tatatttata taactaaaat tttttgcttg
     7141 ttttctaaat ttagtttctg tatattgcat ggcttcaata gcagatattt tagaaataat
     7201 tctagctggt ttaaataaag aaattctaac agttaaaaac attaccagtg ataaaattaa
     7261 aattataaat aaatcagcta tcatttgcca tgctaatggt tgaattatgt aagcaattat
     7321 acaagaagct atagccccta atggaataaa aataattgac aaaatatttc cttgtagaga
     7381 aatagtcttt tttaactgaa aagaagtcat acctaacgat ttcaacttag catacatctg
     7441 aactgaatta acaactgaaa tataaaaaat actatatata acaaaaacgc tagaaaaaag
     7501 aacaactatt ccaactacta aaactgtaat aatctgctca ccatcaatta aatttgattc
     7561 aatatagtta tcatttaagt atatattata tttcttaata cctaaagata aaccaatttt
     7621 ctttaactgt gtcttaattg aagctgaatc tttctctgta ttttcttctg atagtttaac
     7681 taatacatct acagtatcag gtcttgactc gtcaaaagta ttacttttat tttgtaaagg
     7741 tgtagaagct atattcttaa aataatcatt tgaaacgact gtatagtatt gcttggcatt
     7801 atcttgataa taattttcta gaaatccaac aataataaaa tcacattgaa cttctttatt
     7861 cgtaatatca tcataataat ttaatttaat gctatctcca atatctttat ctaaaccaaa
     7921 ttcctgtaaa tatttttctg aaatagcaat ttcattttta tttgtaggta gtttgccttt
     7981 caatggttga aaattcataa gtttaattgc tgtatcatct gcataaacaa tcgacataat
     8041 acagtctgtc tttttattac ttccaattaa tttgtaggtt cctacgccac taaggttttt
     8101 atctttatat agtatatttt ttgctttttc atctacagat ttatatatag catgatatgg
     8161 agatgcatta actaaatcta cagaagcatt atatgaaact aaggtcactg ttaataataa
     8221 acatgtagaa atcattataa tgatacctga aagatatgtt ttaagtttat taatttttaa
     8281 atcatttaaa gctatttttc ttataaatgt gcgattatta tttctaaaca tatactacac
     8341 ctcctagtta acaatttttc catcttcaat acgaataatt ctatcagtta gttgtgcaat
     8401 ttcattattg tgtgtaatca ttacaattgt ttggttgaat tttttacttg tcactttaag
     8461 taaactcagt acatctgaac tagttctact atcaaggcta cctgtgggtt catcagctag
     8521 tataatagct ggttttgtta caagtgctct agctatagca acacgttgct gttgaccacc
     8581 agaaagatta cttggcatat catataattt ttcttcaaga gaaagcatat ttataatttc
     8641 atttataaaa tccttatcta tggtctctcc atccaatcct attggtaaga taatattttc
     8701 atatacatta agtaaaggaa caagattata attttgaaat ataaaaccta tatttctgcg
     8761 tctaaagatt gtaagctgtt catcagtcat agtagataat tccttaccct ttataataac
     8821 ctttccagat gtaggattat ctaatcctcc catcatattt aataatgtgg atttgccact
     8881 accagatgta cctacaatag aaataaattc gcctttttca atagatagag ttacaccatc
     8941 aagagctttt gtaatgtttg aactttgtcc ataatatttc tttaaattaa ttacttgtaa
     9001 aatactcata ataatacctc cttaacatct tgtaaataca gtataaaatc aaaatgttgc
     9061 agaatcatta caaataaaat atttaaaaat ttatttatta ggtaagaaga tagaaaaagt
     9121 cgaccctttt ccaatatctg atgtaacttt aatatagcca ccttgaagag atataatttt
     9181 tcttgaaaga tatagaccta ttccaatacc ttctatgtca tgtacatctt cttcacgata
     9241 aaatctttta aagatttctg cttgatgact ttctgatatt ccttttccat tatcaataat
     9301 atctattttt gtatacataa cccaacattc tgtagctaca cgtatcttac cattagaatt
     9361 tgtgtatttt acagcattat ctaatatatt aaaaatagct tcagaagtcc atttcttgtc
     9421 atgaattata tataaatttg ggtcacaatc aactgtaact tcaatattct ttttttcagc
     9481 atcaaacaaa attccactaa gtgctgtagc aattgtttca taaattgaat ttttcttttt
     9541 agatagtgtg attatacctg tctctagtct ggaggttttt accatagatt gcattaaaaa
     9601 atctaattta tctaattggt tttccatatc cattaaaaaa tccttttgaa tttctgtttt
     9661 aaatgtttga tttagtagag tagtattaat catttttaaa tttgcaatag gagttttaat
     9721 ttgatgagat agatcagaaa ctagttcttg taaatcagct ttctctatag caatactatt
     9781 gcgattttct tgaataactt catacattct aattaatctg tgattgatac gtgataaaat
     9841 agtttcttct tctaattcaa tttcaatatt gtcttcacta ttcatcatac tatctaaagt
     9901 gtgacatatt ttagaacaaa atataactat ttttttatga agtaaaaata ttattaatgc
     9961 tataaatcca caaagtacta ttgtatatat taataataga gcaattagtt ttaaatcatc
    10021 agttattaga taaattgaaa tattaaataa aatagctgta aaaattatac caacagcaat
    10081 tattaaataa atagaactaa aagaaaattt tttaaaattc atttctttca cctaaccaca
    10141 tatatcccat accatatacc gttttgatat acttaagatt gtctttttca attttgcttc
    10201 taatcctact tataacagat gttaaagtat gttcatctac aaagttagca tctatatccc
    10261 agagtttttc taaaagtact ttccttgtta aaatactttt aggattttca gtaagcagtt
    10321 ttaaagtacg atattctaat ggtgtaaata taatttgatt tccatttata ttggcagata
    10381 actcagaaaa gttaatttca agatatccat ccctatagta atcttgtttt gtttttgttg
    10441 taagatgatt taaaaaagct ttaactttct gtttaaataa attgatatta aatggttttg
    10501 ttatataatc caaagctcca agttcatagc ctttaatcat atcacattcc atatcattag
    10561 cagttaaaaa aattattggt atattgtaat tttcctttat ttcattacat aattcaaacc
    10621 cacttttatc tggtaaatta atatccaaaa ttattaaatc aaattcacat tcaaaaatag
    10681 actttagtgc agatttagct gtaaattttg aagtaattat atatccatct tcaattaggt
    10741 tatatgatag agttttattt aatatagaat catcttctac tactaatatt ttttccatac
    10801 agttactcct tttagtttaa tattttataa tgtcaattat agactaaaaa cattgctgaa
    10861 ttgttataaa cgagaaaaag tatttaaaaa tttatatatt taataaagaa cctgtcctat
    10921 gataaattct agtacagatt ctttattaaa taaaattatt tcaaccaaga tggatttcca
    10981 ggtataaata ttcttttacc aaattttgaa gtatactcat ctaaagcttt ttgaataagc
    11041 tcattattac ccttaaatga agtagtattc tttatagttt cgagattttg gctaaatggg
    11101 caagcagata tacacatacc acaatctgta cctaaatatc tccatttaat atagcatgtc
    11161 tcatgttcaa ttacccaatt atattttcca tcatcaccaa ttttaatatc attagaaagc
    11221 gaatgagaag ggcagttaaa agagcatttt ttacagacct tacaaaaatc ttctagtcca
    11281 aaatcaacat attcatcttc taaaagagga atatctgtag taacaacacc taatctaagt
    11341 ctagagccat aatctttatt tgtaagtata gcatttcttc ctatatctcc taaacctgca
    11401 tcacgagcta taaaaacagg cattactaaa taattagcat ccatatgatt tctagcatca
    11461 tatcctaaat ttcttatata gtaacttaaa atcatgccga cgatagatgc atccacatag
    11521 cactttgatg tttctataac ttcacatatc attggagctc tatttatcat ttctttatcc
    11581 atctcacatc caaatacaat tgcatactta tgagttagat taatttcttc accgtaattt
    11641 tcttcatgtc tacccctatt tgtataaata tgatagtctt ttaatttagt tataccaact
    11701 acagaagctc catactgctt tgcaagaccc ttaatttttt ttgtcattga ctttgcatct
    11761 acatctactt tagtatcaga aacttttcct tcgcataaag atttgatatc agataaaaaa
    11821 tcgaatgcac tacttgccat tggagagttt aactcattgt aagtcattgt tccttcacta
    11881 cataaatttg gtctatttct tatactatca tcaatttctt ttttgtcagg atttttctta
    11941 taataatcat tataagcagt actatctttt ttgtaatttg ctcttgcaaa catagtattt
    12001 ctctcatcaa cccttttcaa ttctattccc cctagtaaaa atatatttaa aaaatataac
    12061 tttattgttg tataatttaa ttatataaga ataacgttat attttcaatt taagcgtaat
    12121 taaattggga aatatataac tatatgcttg attatgatgt ttttattttg aaaacaaatt
    12181 taaatacaat ctttttagtg aaataaataa aattaaaata tttttagtat gataatgcaa
    12241 gaaaataatt atttatctaa aaaatctaaa ggagagtaaa attgaactta ttaattattg
    12301 aagatgatat aaacttaaat gaaggtcttt tttatgcatt tgagaatgat ggatttaatg
    12361 tatttaaggc ttatacaaaa caagaaggat taaatatatt taatagtaaa aatatagatt
    12421 ttataatctt agactgcaat cttccagatg gagatggatt tgatgtatgt caaataataa
    12481 gggaaaagtc agatatacca attataatgc ttactgcaag agactcagaa attgatgaag
    12541 taaaaggatt agaaataggg cttgatgatt atattacaaa accattttct ttatctgtat
    12601 taaaggcaag agtcaaagta gcaataagaa aaaaatcgaa taataaagta atatattcca
    12661 atggaataaa attggaccaa aagttactaa aagtatataa aaataaagca tgtttggaac
    12721 ttagctctgt agaatataag cttctaagtt atctaattga aaataaaggt cagatattat
    12781 taaaggaaca aattttacac catatttggg acagcgaaga aaattatgtt gatgataata
    12841 ttgtatctgt aaatataaga agacttagag taaaagtaga ggatgaccca tctaatccaa
    12901 aatatataaa aacagcttat ggaatgggtt atttatggaa tgaggtagaa taatatgttt
    12961 atattagcct ttataatatg tacagttgtt atatttatag tatctactaa aataaatcaa
    13021 agaagatatg atgaattaaa tttgatgtta aatcaaatat tggaaggtaa agaagttact
    13081 tatccagata cgaaggatac aagagcgtca aaaatttctc accaagtaaa aaaaattaag
    13141 gatatgatag aaatagaggt agaacagtca aagtcagaaa aagaagcgat taaaggttta
    13201 atatcaaata tgtcacatca attaaaaact ccactttcaa atataaccat ttattgtgaa
    13261 cttctggaga atataaatat ttccacatta cagaagaagg agtttttaca aaagatgaaa
    13321 aatgaaactt ttaagattga ctggttgtta caatccttat ttaaaatgac taaattagag
    13381 gatggagtta tagaatttga agtagaagaa ctactaatta aagatactct tattcaaagc
    13441 atcagtacta tctttaataa atcagaagct aaaaatataa ggataaatct cgaaccattt
    13501 tcagatataa agttagttca taataaaaag tggaccatag aagcaattgt aaatgtttta
    13561 gaaaatgcaa taaaatactc accatcagat agcacgatta ctatttctgt aataaaaatg
    13621 gagctatata cgaagattac tattaaagat gaaggaatag gtattgatag tagagaacta
    13681 aatgatattt ttaaaagatt ttacagaagt aaaaatgtgg aaaaccaaaa tggcacaggg
    13741 attggtctat atcttacaag attaatttta gaaaaagaaa atggaaacat tatagtagaa
    13801 tctaaactag gaagtggatg ttgttttagt atattcttac agaattgtaa gagtttaaac
    13861 taactttgta ggatgagtgt aattatcgtc acttacaata ttttatagaa catatatctg
    13921 tattgaaagg agaaattaaa tgagtatatt agtgacgaat aatttagtga aacactatgg
    13981 aaaaggtgag actaaagtaa aagccttaaa tggagtaagt atagaaattg aacgagatac
    14041 atttactgct atagttggaa cttctggttc tggaaaaagc acattgttaa atattatagg
    14101 tggattagat aatccaacct caggtgatgt aataattaaa ggaaagaaca tatctaaaat
    14161 aggaaaaaaa gatttgacag tatttagaag aagaaatata ggatttatat ttcaaaacta
    14221 cagtttaatg cccgtattaa acacttatga taatatagct cttcctgtta cattagataa
    14281 aggaaaccat atagaccatg aatatattga aatgctgatg aagacattgg gaatttggga
    14341 taagcgttta aaatttccaa gcgaactatc tggtggtcaa caacagaggg tagcaattgc
    14401 tagagctttg gcaaataaac ctgcacttat attagcagat gaaccaacag gaaatctcga
    14461 tagtaaaact acaatggaag tggtatgttt attaaaagaa agtagtgcaa aattccatca
    14521 aacgatactt atggtaacac acaatgaaaa tatagcacaa gtatgcgact caattatcca
    14581 tattgaagat ggagttgtgg tgaatactgg tggtgaagta ctatgatgtc tctagtaaaa
    14641 ttttcaattc aatatataaa gcattataaa aaacaatcta tatctatagt tttaagcata
    14701 gtactatcag tagcattatt gacgggaatt ggttctttag ttcatagtgc agacaaaagt
    14761 agaattgaaa agataaggga agatagtgga gattatcatt atttctataa agtagataag
    14821 gaacaactta ataaaattaa aaaaaataaa aaatcaaagg aatacattgt agatagactt
    14881 ggcgttacaa aacctaaaaa tcagatagat gaacctttta ttatagaatt tttaaatgct
    14941 gattcttcat atttagacat gacaggtaga aaattattaa aaggaactct tcctagtaaa
    15001 gatggagaga tagtacttga cacatattct ctaaacaatt taaatgtaaa caaagaaatt
    15061 ggaacagaaa tcaatttaga tggtaaaatc tataagttat gtggtatttt atcaagtctt
    15121 tcaaacccag aaactgctat ggagggattt gtaaacgatt atttgcagcc taaagaaaag
    15181 gattcttata tagtttatgt aaaatatgat gaaaataaaa atattaaaaa gcaaaattat
    15241 aaatttatga aaaaattcaa catatcaaaa gaaaaaactt atataaactg gcctctttca
    15301 gttgaatttg gagtaaaacc accaaagaat atagaaaaat ataatatatt tgagttctta
    15361 aaaaatttag aactaaatac aaatgcaatt atattgcttg ttggagtgtt tggtgcagca
    15421 gcagtatata gtattttcca tgtttcaata cttcagagaa tatcacaata tggggtttta
    15481 gaagtattag gcgctaataa taaacaactt ttatttctac tatttttaga gttattttta
    15541 ctttttattg ttggatttcc tataggttgc ttcttaggta ttggagtagc gtctacaata
    15601 tatgaacaat ttccacatat atttttgagt agtgatattg ttccaggagc attttttata
    15661 agtgaaaaat ctatttattt aggcttttta tttatcttat tgttgttaat tttaattgct
    15721 gtacgtgttg tacatcagtt gaataaatac tctagtatag aatctatgaa aaactttaag
    15781 attactaata agaaaaagag aatattagaa agtgtaaaac actctaacat gacaaaagta
    15841 ttatctcata aatacatgac tcaaaaaagg ggaatgttta ttggaatttt gttttcatta
    15901 tcacttggtg gaattatatt tctatgttct agttactcta tacagcttac taaaaacaat
    15961 aatgaactta caatgaaagc tgatgatggt ttaaattctg actaccaaat aactatgcaa
    16021 acaacagatt ttgatgtagg tataccaaaa gataaaattt caagattaaa caatgttgag
    16081 ggaatatcaa aagtctatcc agtaagatat ttatttggag ctatgcaaat taaagaagaa
    16141 caactatttt ggaaaaattt ctttaaacct cttgaaaaag attacaggat aaagactttt
    16201 tttgatggta tttgtacaaa gcaaaatgaa ggtggctatc ttttaaaaac aaatatttat
    16261 ggttataaca atggtatgtt agaaggatta aattcttata ttatagatgg ggagattaat
    16321 acccatgata tggttaaaaa taataaggtt atagttagac tacctatgga tggaactggg
    16381 atgtatgatg cagttgatat aaatccagga gatacaataa aagttaaagt gccaaagaca
    16441 atgaaaccaa caggtgaaat tgttaaattt aaagaagaaa ataagaatga ttatacaaca
    16501 aaagaatttg tagttgctgc tactgtaaaa cgtgtgatgg caaataacat atatttcatt
    16561 ggtgattatg gtatggatat agttatgacg aatgggcaaa tgaaaaataa ctttgatatc
    16621 gaaaattata attcagtaag tataaaaaag acagaagata gtaacagtga ggaattgtct
    16681 gagaacataa aaagtgttgt aagtgatgta aaaagatgta tcgtaacaga ttataccatt
    16741 gctattgaaa aaaataatac ctacttaaat caaaaactct tatttatata tggtattgta
    16801 tttgtacttc ttgcaataag tttgttccat ataatcaata cagtaagcta cttaattttt
    16861 tcaagaagac atgagtttgg aattttaaga gctatgggta ttacggataa taagttttta
    16921 cttatgatga taagagaagg ttttttatat ggattctatg caagtattat aatggtaatt
    16981 ggaagtgtta ttggacaatt tatgatttat tttatggtaa aacgtgtata tttatatata
    17041 aatccaattc ttaaaataaa tacacctcta tacataggta tgattatttt aaatatcact
    17101 attagtatag ttgctgtaat tattccagta agacaaattt tgaagagtga tattatttca
    17161 gaaataaata agagttagag ttatataaat ttttaagatt atataacttt atgacaatct
    17221 tgtttaaatt atttaaatat atcttatctg tgaacattaa ataaacctct taatagatat
    17281 aactcttgaa aagtcatatc tattaagagg ttttaataaa ttttcatgtt aatatatata
    17341 atccaaaaaa attaaatctt attaaaacat actattttgt atagttatcg aaatatcctt
    17401 gtatgtaaat aataggagta cccttatctc cacttccaga tgttaaatct gctaaagaac
    17461 ctataaggtc tgttaatctt cttggagtag taccttgaga aaccatgttt cctgttaaat
    17521 catcagattt attatctttt tcaactatgt attttgaaat tgcttctttt aactcatcac
    17581 cagataagtc agcaaaatca ttatctgcta agtattttaa ttttacttcg tttggagttc
    17641 cctctagccc ttttgtataa gcaggagata ctactgggtc agctaattcc catattttac
    17701 ctacagggtc tttaaaagct ccatctccat aaaccattac ttctacatct ttccctgtta
    17761 tttcttttat attaccttgg attttgttta caacttcatc gcaatttata gggaataatt
    17821 ttactgtttc ttctgtagct ttgtttgaac ctaaaagtcc atactggtca ttgtaaccac
    17881 taccatctac acttgctgtc attatatcat caagtgaata tactttttta gctccatttt
    17941 gtcttaaaat tctctttgtt ctttgtcttg tatgtatatc acaagttaaa atacttgttg
    18001 tatagttaag aatagttttt ggattgtttg caaatactat ttctacctct gcaccacaat
    18061 ccttgattaa attcttataa taatcaacat aatctacacc agtaaatgta tgcttgttat
    18121 atccaaatag ttctctgtat ttttcttctg ttaaaacatc tgaccaagga tttattccct
    18181 tatcatcaag ttcatcaata cttattaagt gatttccaac ttcatcagaa gggtagctta
    18241 acattaatac gacttttcta catccttttg caattccttt taaacatata gcaaaacgat
    18301 ttctacttaa aattgggaat ataaccccaa cagtatcatc tccaaattta tcttttacat
    18361 cttttgcaat attgtcaacg tgagcataat ttccctgtgc tctagctaca acagcttctg
    18421 taacggcaat tacatctttg tccctaactt caaagttttc actttttgac gcatttaaaa
    18481 cagaatcaac aactatttta actaagtcat ctccttgtct tataataggt gctctaaccc
    18541 ctcttgaaac agttccaact aatctatcca ttatctaaaa tcctcctaaa gaattaatat
    18601 tgtcatatta gtaccatgta tattataat
//
LOCUS       DAEPOG010000067        18448 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEPOG010000067 DAEPOG010000000
VERSION     DAEPOG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.067"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            42..767
                     /locus_tag="KR432_001928"
     CDS             42..767
                     /locus_tag="KR432_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640465.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            897..2822
                     /gene="mngA"
                     /locus_tag="KR432_001929"
     CDS             897..2822
                     /gene="mngA"
                     /locus_tag="KR432_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH0640466.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            2966..5581
                     /gene="mngB"
                     /locus_tag="KR432_001930"
     CDS             2966..5581
                     /gene="mngB"
                     /locus_tag="KR432_001930"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0640467.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            5863..7017
                     /locus_tag="KR432_001931"
     CDS             5863..7017
                     /locus_tag="KR432_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBH0640468.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(7140..9053)
                     /locus_tag="KR432_001932"
     CDS             complement(7140..9053)
                     /locus_tag="KR432_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0640469.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            9301..9618
                     /locus_tag="KR432_001933"
     CDS             9301..9618
                     /locus_tag="KR432_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0640470.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            9626..10873
                     /locus_tag="KR432_001934"
     CDS             9626..10873
                     /locus_tag="KR432_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0640471.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            10902..11255
                     /locus_tag="KR432_001935"
     CDS             10902..11255
                     /locus_tag="KR432_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0640472.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            11277..12734
                     /locus_tag="KR432_001936"
     CDS             11277..12734
                     /locus_tag="KR432_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0640473.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            12822..13439
                     /locus_tag="KR432_001937"
     CDS             12822..13439
                     /locus_tag="KR432_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640474.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            13736..14488
                     /locus_tag="KR432_001938"
     CDS             13736..14488
                     /locus_tag="KR432_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640475.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            14590..15510
                     /locus_tag="KR432_001939"
     CDS             14590..15510
                     /locus_tag="KR432_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBH0640476.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            15513..17429
                     /locus_tag="KR432_001940"
     CDS             15513..17429
                     /locus_tag="KR432_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBH0640477.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            17449..18318
                     /locus_tag="KR432_001941"
     CDS             17449..18318
                     /locus_tag="KR432_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0640478.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
BASE COUNT         7155 a         2018 c         3392 g         5883 t
ORIGIN      
        1 gaattttact tatgaataat aaaatagatg gaggagaaac tatggaattt tatgaaagag
       61 caaaaaaata tgagtataaa tttacagata cagaagacat gataattgag tatatgagaa
      121 aaaacaaaga agaaatagta aaattatcaa tacaatcttt agcagataaa ttttttactg
      181 tcccaaatac catagttagg ctatgtaaaa aaatggagta tgatggtttc tctcaactaa
      241 aaaacaattt aaaagaagag ttaaaaaatg aggataaaga gccaaaaaat ggttttgaat
      301 cagatataaa aaagacatta gagctaatag attatgaaaa aataaataaa gtagcagata
      361 taattcaaaa atctaggagt attatagttt atggaatagg tcaaaacatt cctttatgtg
      421 agatattagt aaaagaaatt agaggtgggc atgataatat aacttactat agccaaagac
      481 atgaagtact tcatgatata ggaaaattag gtgaacaaga tatgtttttc gcgcttagtt
      541 taagtggaga aacgaaagaa gttatagaag cagcaaagac agcagttaat aagggttgta
      601 aggtagtagc tctaacacat atatgtgaaa atcaattgca acaaattgca gatataagtt
      661 tatattgcta ttcaacaatt aaacaagtaa atgggtacaa tatatcagat aaggttccaa
      721 ttatgatgat tatgagagca ataatagaac aatactggtt aagatagaga tttgtgtata
      781 aatacacaaa tccttatttt ttaagaagaa attgtgtgta taatttcaaa aaaacatttt
      841 catgatttat tttatgctat attcataaca ttaaatgatt taaggaggtc gtaattttgg
      901 aatttaagaa aatcacaaat gaaaaccttg tttttttaaa acaaaaaata aaaacaaagg
      961 aagacgcaat aaagttctta gcaagaaagc tatatgatga aggcaagtta acatcttatg
     1021 aggaatattt agaagctgta atgtacagag aaactttatc tccaacaggt tttgaaagag
     1081 ggcttgcaat tcctcatgga aaatctaagt cagttaaaga agctgcattt gcagctatga
     1141 ccttagagac accaatagaa aattgggaaa gcgtagactc aaataataaa gtagagctaa
     1201 tatttttaat agcagtacca gaaagtgaag gtggaaatac acacatacaa atactttctg
     1261 agttagttac tagactgtct aatgaagatt atatgaatat gttgttaaat tcaaaaacta
     1321 agaaagagtt atttaataac ttagatataa aagaaaagaa agaacaaata aaagaagaga
     1381 ttaggattca aaatgaatca aaaaaaattg tattagcaat aacagcatgc cctgcaggta
     1441 tagcacatac atatatgtca gcagaagcat taattaaagc tggcaaagaa ataggtgtgg
     1501 atgtgtatgt agaaaagcaa ggagctaatg gtatggtgga ccctcataca ccagatataa
     1561 taaaaaaggc agatgcaata atatatgcaa cagatgtagc acctaaaaat acagaaagat
     1621 ttgaacattt gccaaacata aaaacgtcag tagcagctcc actaaaaagg gcaaaagaaa
     1681 taatatacga agctttagaa gtagcacaaa aacaaggtaa aggtgattat atagaaaaat
     1741 catctgatat atcatattgt gaaaaatcat catggaaaaa agatgtaaaa tcagcaatac
     1801 ttacaggagt atctcatata ataccactta ttgtggcagg aggtatgata cttgcatttg
     1861 cagtattagc atctcaagca tttggtttac aagaactata caatcaagaa ggttcttggt
     1921 tatggcaatt aagaaagctt tctggaggga tgttaggtac attgatggtt ccagtattat
     1981 cagcatatat gtcttactca ctagctgaaa aaccaggtct tggaccagga tttgcagcag
     2041 gtctagcagc tgatttaata ggtagtggat ttcttggagg tatgttagga ggtttacttg
     2101 ctggttatat agtaaaattt ttaaagaaga atataccagc caaaggcaca ttagctggat
     2161 tcgtaagttt ttgggtatat ccagtagttg gttctttggt agtaggttta ttgatgttat
     2221 ttgtaatagg aaagcctgta gcagcactta acaatggtct tataaattgg ttaaatggga
     2281 tgactggaat aaatgcaata ctactaggtg caataatagg agctatggtt tcatttgact
     2341 taggtggacc aataaataaa gcagcctata catttgcaat aggagcaata gcaagtggaa
     2401 attttatacc ttatgcaata tttgcatcag ttaagatggt ctcagctttt ggagttacaa
     2461 tagctactgt tttaggcaga gatttatttt cagaatcaga aaaggaaata ggaaagcaaa
     2521 cttggatatt aggattggct ggaataactg aaggtgcaat accatttatg atagaggacc
     2581 caatccatgt tataccatca ttagtagctg gttcagccat aactgggtct atagtagcat
     2641 tcttcaatat aggtcttcaa gtgcctggag cggggatatt ctcattggcg cttttagagg
     2701 gaagtatgtc aggatttatt atagcatcaa tatggttctt tgcggcagta cttggagcta
     2761 taatatcagc aatattatta ataataacta ggaaacaaaa aaatcaaaga aagatacatt
     2821 aaaagatacg ttattagata tttaaatcaa aggtttaatt atagtgataa aataaatatt
     2881 aaaaaattgt gtaaactcag tagtattttt attggatttt taattagaca gaaaatatta
     2941 tcagataagt tttggaggat aagcaatgaa aaaaaatgta cacatagttc ctcatatgca
     3001 ttgggataga gaatggtact tctcaactga ggaatctaga atattacttg taaataatat
     3061 ggaagaaata atggatatgt tagaaaataa tcataactat ccatattatg tgatggatgg
     3121 tcaaactgct atattagaag attatttagc agttaagcct gaatgtaagg agagaataaa
     3181 aaaattagtt caagaaggaa ggctaataat aggtccttgg tatactcaaa ctgatgaaat
     3241 ggtagttggt ggagaatcca tacttagaaa tttactgtat ggaataaaag attgtgatga
     3301 atttggagaa tatatgaaaa taggatattt accagattca tttggtcaaa gtgctcaaat
     3361 gccgcaaata ctaaatgggt ttgatataaa atactcaatg ttttggagag gatgttctga
     3421 aagaaaagga actaataaaa cagagtttaa ttggaagagt gatgatgggt cttctgtttt
     3481 ggttcaaata cttccattag gttatgcaat aggaaaatat ctaccaatga atgaagatga
     3541 gctaagaaca agaatggata aatatttacc ggttcttgat aagggtgcaa ctactgacca
     3601 tataatactt ccaaatggac atgaccaaat gccaattcaa aagaatatat ttgaagttat
     3661 atataaatta aaagaatgtt atccagagag aaaatttttc ttaagtagat atgaaaacat
     3721 atttaaagag ctagaaaaaa ataaggatat agatacaata aaaggggaat ttttagatgg
     3781 gaaatatatg agggttcata gaagtatatt ctcaacaaga atggatataa aatcagcaaa
     3841 tgctagaata gaatcaaaga taaccaacat attggagcca ctagcttcta ttgcatattc
     3901 tctaggattt gaatatcatc atggattaat agagttaatt tggaaagaaa taatgaaaaa
     3961 tcatgctcat gacagcattg gatgttgttg ttcagataaa gttcaccagg aaataatgaa
     4021 tagatttttc ttagctgagg aaaaagtaga ccaattaata acattttaca aaagaagaat
     4081 agttgatgca atgagttgtg aaatggcttt agataagtta gtagcattta acttaatgcc
     4141 ttacaatagg aatgaaatag tgaacgccca agttataact aaaatgaaag catttgaaat
     4201 atttgataaa gataataata agttggattt tgaagtggta cataaagaag taatagatgc
     4261 aggattaata gaccgtcaaa ttgttcatta tggaaactat gattcattta tggtatatac
     4321 aataaacttt aaagataaaa tacctgcaat ggggtataag gcgtatatga taaaagaagt
     4381 tgaacatatg ttagaaaaag cttatgaagt atgtgacatg gtagataatg acttttatac
     4441 tattcaagtt aatgaaaacg ggacattaaa gatatctgat aaaaaattaa ataaaacttt
     4501 tgaaaatgta ttattgatgg aaaatggagg agatgaagga gatgagtatg atttttcacc
     4561 acttccaaat gagaaattaa tatttaacga taatatagta gcaaattcca ctataaagaa
     4621 aaatagattt aataatgaga taaagataaa ttatagatta aaagtaccaa aagatataca
     4681 agctagaaaa aataatagag cagattcatt tattgacttt gatatagtta taaatgttcc
     4741 aaatgataaa gctataataa atgtagtatt caatataaat aaccaagcaa gagattgtag
     4801 ggttagaact tatataccaa caaatatagc ttcaaagttt tctgtgtctg ataatcagtt
     4861 tggacatata aagagaagtg tgtatgatga tgctatggaa gtttggcaac aggaagaatg
     4921 gagtgaaaga ccagattcta tatatccaat gctaacattc gttggattgt ctgacaaaga
     4981 gcatggagta gctgttctta caaacagtac aagagagttt gaaatagtag ggaaaaaatt
     5041 tgacacaata gctataacat tatttagaag tgtaggattc ctaggtaaag aagaaatggc
     5101 aagacgccca ggtcgtccat caggaataaa gttgcctact ccagattctc aaatgatagg
     5161 agatataaaa atagacttag caatagtcac tcatgaaaaa tctacactag aagcaaatgt
     5221 tgcaaatatg gctaaagagt atttaacaaa aatacaaact tataataaaa taccatataa
     5281 tgctatgaaa ttgaatcaat cagaaataga agtagattat tcatatagtt tacttaagga
     5341 aactaataag gaccttgtgt taagtgttgt aaaaaaggct gaaaaagaag aggcattagt
     5401 agttagattt tacaattcta aagaagaaac aaaatcagct aacgtagtat ttaacaaaaa
     5461 tattaaaaca gctaaatgga ctaacctaaa tgaaaaagaa ttagaatcta tatcaataga
     5521 taataactct gtaaatgtag agtgcctaat gaaccaagtt aaaactatat tggttaaata
     5581 gtgctaaaaa tgattagata gtgctaaaaa ggaattatct taaaatagag ataatcctcc
     5641 ttatcattgt tgaaataata aacatatatt taattttata caaaaagagt gtctcatgaa
     5701 ctaaaaagtt cattttgaga cactcccttt tttataagat aaaagaatat taagggtata
     5761 agttgtatac aaagtgaaat atagagtata aaataaaaga taaattaagc ttataaaatt
     5821 atatatttaa aataaagagt aaataaaaag gagtagataa atatgaaaat attaatttct
     5881 atagattctt tgaaaggaag tttatcttca atagaggcag gaaatgcaat aaaaaagggg
     5941 atattgaagg ttaaagaaga tgcgcaagta aagatattgc cacttgctga tggtggtgaa
     6001 gggacagtag atgctttagt tcaagggatg aatggtaaaa aagagactat agaagttact
     6061 ggaccaatag ctaaaaaagt ggatgcaaca tatggattat taaaaaatac aagtacagca
     6121 ataatagaaa tagctcaagc atcaggatta acattggttc cgactgaact tagaaatcct
     6181 ttatacacta caacttatgg tgttggggaa ataataaagg aagcaataaa taaaggctat
     6241 agaaatttta tagttggaat aggtggaagt gctactaatg atgctgggat aggtatgctt
     6301 caagccttag ggtttgagtt ttatgatgaa aataacaagt tagtaggatt aggtggaaaa
     6361 gttttaaatg aaataagaca tataaaaata gaaaatagat taaaggaatt agatgaatgt
     6421 aagtttaaaa tagcctgtga tgtaaataat ccgctgtttg ggaaaaatgg agctgcatac
     6481 atatatgggt ctcaaaaagg tgcaacatca gaaataattg aggagttaga taatgggctt
     6541 agaaattttt ccaaagtagt taaaaattac ttaagtaaag atgtagctaa tgtagaagga
     6601 gcaggagcag caggaggatt aggttttgct tttttagcat ttttaaattc aaaattagaa
     6661 tctggaataa aaataatatt agaagaaatt aaattagagg aagagttaaa agatgcagat
     6721 tttgtcataa ctggtgaagg tagattggat aatcaaactg ctatgggcaa ggctccaata
     6781 ggagttgcaa aactggcaaa aaaatatggg gtaaaagtaa taggtttggc tggagccaca
     6841 acagaagatg cagttaaatg taatgaagag ggtatagatg cttatttttc tatagtaaat
     6901 agagctatga caatagaaga agcaatggat aaagcaactg ctagtgagaa tatgacagct
     6961 acaacaacac aaatattcaa tcttataact agtatacaac aatcaaaaaa tatgtagtat
     7021 taaagcaaag tatattaggt aaaaagctgt cacatattaa tttagacttt ttagtgtgac
     7081 agcttatcta ttttgtgtta ataacttaag aaacatatat tggaaataaa ttaatggatt
     7141 tagatatttt gaataagata agctattcta ttcataaaac tactgtagtt atctgcctta
     7201 gtaagttctt taacattatt ccactcagat aatatttcaa taaagctttc aaatatgtaa
     7261 ttgaattttt ctttttcttt ttcatttata ggtatcatag ctataatttg aactttttcc
     7321 tcaccccatt taactggttt atcgttaaca attaaacata ctccagtttt taaagcatcc
     7381 attttcatgc tatgaggaat tgctacattg ttattaaatg cagttgaaga cattttttct
     7441 cttataagaa catcatctat ataattgggt ttgacatatt tatttttgta cagaatattt
     7501 cccataaatt ttatgtaatc tttagcacta tctaaatata tgtttttcat aaacaaagat
     7561 tcactaaagt acatttctaa atgattttgt acagtaagta tattttttct atctttaatc
     7621 tgaataaatt tactttggat attatcataa tcttttctgt ttaggtaagg atttacataa
     7681 acaaaaggta tagggcatct atcactaatt ggtaaagtag taataaccat gtctacctta
     7741 ttgtcaaaat caagattatg agtatctgaa aaaatttgaa taatttctat ggattcatta
     7801 aatctcttct cgaggtctct tactattttg aattgtaaat catagtaatt tggagtaatt
     7861 aatgcacaga gaattttatc ttctagtaat ttttgtctat caaaaaatga acctacatga
     7921 agagatatat acgatatttc atcttcatct atttctatgt tttctaaaat ttgaatttga
     7981 ttacaaatat aaacagatat atcatatatc agagggtagg aatcttttaa cttctgaggt
     8041 atttgatttc tcaaaacttg attattttta gccctactga ttaaattttt tacatgaaga
     8101 gtaaatttta caacaaattc atcatcagat aaatctataa agtataagtc atatacattt
     8161 ttaattattt ttttagttaa tttaacaaaa tgttcatcta tataatttgg caatgactta
     8221 gtatccattt catgataatt taagactgtg gttttgtttg ttaactgaaa tactaagtag
     8281 tataattctg cactagttaa agttaaattg tattcagact ctaaaaaatt tgatatatct
     8341 cttgaaatgc ttatatcatt attattatct atgtagttta ctacttcgac agattctatg
     8401 tagttattct tttttattct gtctatagtt atcattaaat gaagaagtat gttatttata
     8461 gcgtactcat ttataaacaa attgtatttt tttaatatat atattatatt ttctttcagt
     8521 ttggtcaaat tatactcttg atatattttt tgtacatcaa ataagcttaa aaaatcactt
     8581 cccgtttctt taaatattat agagctcatt aatttgcgct ttgcagactc atttccaatt
     8641 agtttcaata aaaatttcga ttgctttatc tttaaattat aacttcctat aaatttatta
     8701 gctttgacta tatccccttc aatagtagct ggactaacaa acaattcttc acttaaatca
     8761 aatatattaa ttccattact attaactata agttctttta aaattaaatt taacctgtta
     8821 tcaggtgata tattttctga actatcaacc ttggtttgac agttattatt gagatttact
     8881 ttatatcctt ttttagagga taatataaat tcaccagtag aattaatttc atttacatat
     8941 tttctaatcg aacgtgttga tacattaaga taattagcta atgtggatga atctatccaa
     9001 tcatgttgcg tttttatgaa gtttaaaaat aactcttttt tagatgtttt cattagattc
     9061 cctctcatag ttttaaaata aaatctgtat gatatgatat ttatataatt gaatgtacgt
     9121 attataatgt acattatact atatatttat gtgtctggtt aagagtaaat gatttcctat
     9181 tattaggaaa tatatttatg tgaaaggaaa ttatttagga agtacaatat aaatacaatc
     9241 aaatcaaata aaaattcatt aagaaataaa ttaatgaata aaaatactgg aggtattgtt
     9301 atgaagaata taaaagtgtt gttggcatgt ggctcaggag caagtacagg ttttatggca
     9361 actagtatga gaaagagtgc aaagaaaaaa ggcatagaag cagatataaa agctagaagt
     9421 gagtcagaga tagataaata tttaccagaa atagattgtt tattattagg accacattta
     9481 gattatatga aagatgaaat acaaaaaaaa gctgatgaat ataatgttcc agtagcatgt
     9541 atatctaaaa cagcatatgg aagattagat ggtgtaaagg ctttagaaga agcattagaa
     9601 ttaatattaa acaaataaaa tatctatggg gagattgttt atgaataaat ttatagattt
     9661 gttaagtgaa aagtttactc ctgttgtaaa taagataact aagaatatat gggtacaatc
     9721 agttcaatca actataatga aagttttgcc tatggtattt gtaggttctt tagtaactat
     9781 agtttctgtc ttgaagaatt acataagttt cttacctgat ttaagtccaa taaatcaata
     9841 tacatttgga cttttaggac tatttatagc atttttatta cctatggaaa taatgaagaa
     9901 taagaaattt gaaagtatga gtgtagtagc tggtcttgca ggtgctggat taatgttaat
     9961 gatgattcgt ccagagatta cgaatgaagg agctatattt aacttcaata gatttggagg
    10021 agaaggaatg cttgtagcac tagttgcagg attattctcc ggattaataa tgtctttatt
    10081 tgggtctttc tcattttttg gtgaagattc agcacttcct gattttgtaa gtaagtggtt
    10141 tgatagtatg ttacctatag cagtaataat gtttataggt tggggactaa tttatggact
    10201 taactttgat atgtttgaaa taatagttag gatatttaaa ccagtatcag atatagcaca
    10261 aacattgcct ggatttgtac tattaaactt cttagtagta tttttatatt ctttaggtat
    10321 aagtggatgg gtattatccc caataactta tgcaattcag cttagtgcaa ttgcagctaa
    10381 tgcagcagct gtagcatcag gagggcttgc aactaatata tatacttatg aagttgttca
    10441 aacaggttgg ataactttag gtgggtgggg agcaacatta ccactagtgc taatgatgtg
    10501 tttatctaga gctggaagaa taaaagcaat cggaagagcc tgtatcgttc cttctataat
    10561 gaacataaat gaaccagtaa tgtttggagc tccagtagca tggaacccat ttttaatgtt
    10621 acctatgtgg ataggaacag tagtaatatc aataactact tacttatcaa tgagtatggg
    10681 atttgtgaca attccgagta aggtgtttaa tttatggtat cttccttttg gtatatctac
    10741 ttttatggta aatcaagatg ttagagggct tattgtttta gcaataaata tagtattgct
    10801 attcatgata tggtatccat tttttaaagc ttatgaaaaa gaagaactaa aattagagag
    10861 tcaagaagct taaatttaga tgttgaaagg aaattataaa tatgacaact gaagaacatg
    10921 taatagatga agaattagta gaagtagcaa tgcaaataat acttcgtgca ggtgaggcta
    10981 gaacagaaat taaacatgct cttaatgatt tagaaaggtt tgattataaa aatgctgatt
    11041 taaaactagc taaagcaaaa gagtttatga cagaagcaca tagagctcaa actaacatta
    11101 tacaagggga agctagtgga gaaaaaagag cacattcact tttatttgca catgctcaag
    11161 atactcttat gacaatattc agcgagttaa atataactat gtctttagta ggaatagtaa
    11221 agtctataga atataaggta aataatagta aatgatttaa ataaataaaa taaggagtga
    11281 ttaaattgag taagtttcct aaagattttt tgtggggtgg agctgtagca gctaatcaat
    11341 tagaaggtgc ttggaatgaa ggtggcaaag ggtggtgctt agctgatgta catatgtatg
    11401 ataaagatag agatatatct aaacattacg agtctgatat gactgtagat aaaataaagt
    11461 ttgctatgaa agataataat gggtattatc ctaagcgtaa tggaatagac ttttatcata
    11521 catacaaaga agatttaaag cttttgaaag aactagggtt aaagtgtttt agattttcaa
    11581 taaattgggc tagaatattt ccaaatggag atgatttaga gcctaatgaa gagggattaa
    11641 agttttatga taatcttata aatgaagtaa tagataatgg tatggaacct attgtaacac
    11701 tactccatta tgagactcct ttgaatttag tattaaaata taaaggatgg tctaatagaa
    11761 aagtagtgga tttctttgtt agatatgcag aattaatatt aaatagatac aaagataaag
    11821 ttaaatattg gatagttata aaccaaataa atttaatgta tcatgaatca tttaattcag
    11881 ttgcattttg tgtagaccag gtagatgatg tagaagaggc caaatatcaa gctattcatc
    11941 atcaattcat ggctacagct ttaactaaga agcttggaaa agaaataaat tcagatatga
    12001 tgatagggat gatgatggca gattgtacat cttatccaga agattgcgac cctgaaaatg
    12061 tagtatttgc tttaaaaaga aatagaatac aatatttttt tactgatgta agttttagag
    12121 gggagtatcc agcttatatt aagagattct tcaaagaaaa taacataaat atagttatgg
    12181 aagaaggtga tgataaagtg ttaaaagaga atactatgga ttttctagct ctatcatact
    12241 attactctac aacagtatca gcaagtaaaa ataccatgaa tccaattgat aatgtaaaaa
    12301 atcaatactt aaaagcaaat ccttgggggt gggcaattga ccctaaagga ttatacaata
    12361 ctttatctca atattatgat agatatcaaa agccaataat gatagctgaa aatggatttg
    12421 gaatgcatga taaatttgaa gatggaaaag ttcatgatag ttatagaata gattatttag
    12481 gaaaacatat agaacaaata ggtgaagcta tagctgatgg aaccgaaata ttagcttatt
    12541 gtatgtggtc gccaatagat atagtaagtt gttcatctca agaaatggaa aaaagatatg
    12601 gatttatata tgtagatata gataacaatg gaaatggaag caaagaacgt tacaaaaaag
    12661 atagttttta ttggtataaa aatgtaataa gaactaatgg agaagaatta aaagaaaaag
    12721 tagatagttt ttaaatataa ataatccctc ttataaatac tatgttatta atacagtttt
    12781 ataagaggga ttataattat aaataaggtg gtttattaat tatgaatgtc ataaaaaaat
    12841 ttatatttgt aattttaatt ataaatatac tgggatgtgg tatatctctt atacttcagt
    12901 caagccttgg tagtgatgca attacacttt taaatgaggg tatacatata aagttaggta
    12961 taagttatac aatagcaggg ctaatatata atggaactct attacttata gcattgttat
    13021 tgaataaaaa aagtttggga ttaggaagct gtgtttatgt attagtagca ggaatattta
    13081 tagatttgta tatatattta ttattagatt ttaatataag tagtttaggg attatcatta
    13141 gaataatgag ctttataatt ggacatatac ttatgtgtag tggatttgca atgcttataa
    13201 aatttgacat tggaatgagc cctttagatg ctatattaat ctatattgag ggcaaattta
    13261 agtatccata taaagtttta aaaactatag cagatttaat atttttaatt ttaggtgtat
    13321 atttaggcgg aaatttaggg tttggaacta tattttcaat attatgtact ggaactacaa
    13381 tttcaattgt tagtaagatg atttctgtta ataaaaaaag gtattactca agtatataaa
    13441 ttgataagtg agagtatagg ataatgttta tgaatcattt tcaataattc gagttaattt
    13501 gaggctgtaa gaattggagt tactcagttt cagtaattga gtaactccaa tttttattac
    13561 aaaatactct aataattaca taattaattg aagcataaaa ttattatatt ataaatttac
    13621 atatatagac attgactaaa tcttaattgt atataaaaat aagtagttaa gaggtaatat
    13681 attatattac tcgataaatt tagacaaata aaacttatat aaggggagat aacatatgat
    13741 aattgagcta aacaaaagta attctgaaaa tctaacgaag acggaacttg aaataattaa
    13801 atttataaat gaaaatgaat caatattacc agaattatct atagtagaga ttgctcatga
    13861 aacatattca tctccagcaa ccgtttcaag agcaattaga aaatgtggtt taaatggatt
    13921 caatgaactt aggtatagat taacagtaaa agatgaaaat gatgatatac aaaatatggg
    13981 ggatataata aataaatcct ttatagaagc acagagagtt atagaacaaa tatctttaac
    14041 tacactaatc aatataataa aaactataaa taaatctagt aagatatata ttttggcaag
    14101 agggcttact gaatatgtag caaaagagtt tagtcttaaa ttgcaattac ttgattttaa
    14161 tgttgttttt ataaatgacc ccaacataat gaaaataaaa tctaaatcaa taaaaaaaga
    14221 cgaacttata atcatatttt cattgaatgg aaataccata gagcttgtag aatcagcaca
    14281 gaatgccaat ttagttggag ctaaggttat aacttgctgt tgtagtgaaa gtgcaaaact
    14341 tataaagtat tcggatttat atattgtagg atacaaacat gaacatatat caatttctga
    14401 atatgaagta tcttccagat tgcccttgta tattatatct aggataatta tagattatat
    14461 ggtcagatat aataattctg aaaattaaat tgaaaagtct atgaaaatcg attttctatt
    14521 tgtgaaaaga ttttcataga cttttttatt ataatgaaaa catacacagc acaagcaagg
    14581 gggaagacaa tgatatatac attaactacg aatcctgcaa tagatatgaa tatatctact
    14641 aatgggataa agagaaaatt agtaaatcgt acatcaaatg ctgtatattc tcctaatgga
    14701 aagggattaa atgtaacttt tgtactaggt cattatggta tagaatctaa gatattagga
    14761 ttttttggag gattttcagg aaaatatata gttgaagaaa gcgaaaagag aggatttgac
    14821 gtattaccta cttgggtaga agatacaact cgtataaata tatttttaaa tgatggaagt
    14881 gatgaattta aatttgtaaa ctctgggtca tatgtaaacg aaaaacaaaa actagatatg
    14941 ctagataaga tacagtcatt agcagatata agttacttat caataagtgg aagtctacct
    15001 cctggtattg atgataatta ctatgaggat atatttaaaa tttgtaaaaa taaaagtata
    15061 aagacaatac tcgatataag ttcaccaaaa ttaaaggagt tactacagta taatccatat
    15121 ttgataaaac caaatgatga agaaatcaaa gatatatttg gaattattgt aagagatgaa
    15181 gaagatatta aggatgtact taaattactg catttaaaag gtgctcaaaa tatattgcta
    15241 actttaggag aaaaaggttc ttatttttat aatggaaaat caatttatta tgctagtgct
    15301 caaccagtta cagtagtcag ctctgcatgt gctggtgatt ctgcattagc tgctttttta
    15361 agtatatggt tggaaaatcc agaacatatt gaagaagcat taaaaaggtc agcagcaaca
    15421 ggagcaagtg tggctgaaag taatggtata ggtaatttgg agaatgtgga agattatata
    15481 aaaaatataa gagtcagaaa ggtggtataa agatgggaaa gaaaatgatt ctaggggtaa
    15541 caggttgtcc aactggaata gctcatactt ttatggcaga agaagcatta aaaaattcag
    15601 caagggaatt agggtgtgac ataaaagttg agacgaatgg agctataggt gtagaaaata
    15661 agttaacagc aaaagatatc gaaatggcag atgcaataat agttgcttgt gataaaaatg
    15721 ttgatatgga cagattcaat ggaaagccgg taatagaagt acctgtcaaa gaaggtatac
    15781 ataaggctaa tgaacttata caaaggtgta ttgatggaaa agtagctatt agaaaaggaa
    15841 acttatcaag cagtaaaatt gcagaagaag gaaatttatc gtttggtcaa aagttgtata
    15901 aagacttaat gaatggtgta tctcacatgt tacctttagt agtagcaggt ggagtactta
    15961 ctgctatatc atttttatgg gggatttatt catttgaccc aaattcagaa caatataatc
    16021 aaatagcagc tacattaaaa tctgttggtg gatattctat gaacttgatg gttcctgtat
    16081 tggcagcatt tatagctcaa tctatatcgg gaagagctgg tatgttggca ggacttgttg
    16141 gaggaatgat atcatttgat acaggttcgg ggtttttagg tggaatcata tcaggatttt
    16201 tagcaggtta cttagttaaa ctatttgtac atttgttaag aaaattacca cgacaattag
    16261 aaggtcttaa atcgattttt ataattccta ttgtcagcgt aggtcttgtg ggtgtagcaa
    16321 tgttgttact tggtggacca tgttcagcac ttaacaatgc tatgatgaac tttctatcgg
    16381 ggttacaaaa ttcaagtcct ataattttgg gaatagtaat aggatgtatg tcagcatttg
    16441 atatgggagg tccagtaaat aaagcagcat atgtaactgg aactatgctt ctaggacagg
    16501 gaaattattt atttatggca ggtgtatctg cagcttgtat aacaccacca ttggtaatag
    16561 caatagcatc tactttattt aaaaatagat ttacagaaga agatagagca gcaggattaa
    16621 tcaattatat actaggtagt actcatataa cagagggggc aatacctttt gcagcaaaaa
    16681 atccattaaa agtgttgcca gtacttatga ttgcatcatc aatatcatca attttaactt
    16741 atataatgaa aatagaagta ccagctccac atggagggtt tttggttcta ggattagtca
    16801 ataagccact tttatgggta gggtgtatat ttgtaggttc attagtaggt gctgtacttt
    16861 atatgatggt tacacctaaa gtagtaaatg agaaaaatac tatagaaaat acaaatgcaa
    16921 caaaagatgg acataaaaat gaaagtgcag aatcggaaaa cgtatctaat aatatctctt
    16981 tatatagcga agaaacagta attttagatg ttaagggaaa aaataaaaca gatgtaattg
    17041 atgaaatgat tggaatactt gataaatctg gtgttctttt agataaaaag aagtttaaag
    17101 aagaaatcct taaaagagaa gaaatatcat ctacaggttt tggtatgggg atagcgatac
    17161 cacatgcaaa aacagatgca gtaaaggtac cacgtgtagc agtaggtgta tcaaaagaag
    17221 ggtttgactt tgaatcagaa gatggaagct tggcacatat tatatttatg atatcagcta
    17281 cagataatgg tgataattta cacttaaaaa cattatctca actatcttca aaattaatga
    17341 atgaagaatt tttaaatgag ttattaaata gcaaaacaag tagagaaata gtatcaaaat
    17401 taaataacga agaaatcaaa tcagtataaa catataggag gagaaattat ggcattagtg
    17461 acaactaaac aaatattatt agatgcacaa gaagggcatt atgcagtagg tgcatttaat
    17521 gttgaaaata tggagatggt tatggcagta atagaggctg ctgaagaact taaatcacca
    17581 gtaatattgc aaacaacacc ttcaacaata aagtatgcag ggctagatta ttatttagcg
    17641 aatgtaaagg tagcatcaga gaaggcaagt gttccagtgg caatgcactt agaccatggt
    17701 tcaagctttg gactagctat gcaggcactt agagttggat atacttctat tatgattgat
    17761 ggttctcatg aaagttacga agataatatt tcaatatcta aatctgttgt agatgcatgt
    17821 atgccttcag atatacctgt tgaggctgaa ttaggcaaag ttggaggtaa ggaagacgat
    17881 ttagatggtg gtgatggtgg agcatataca gacccattag aagcaaaaga gtttgtagag
    17941 agaacagggg tatcatcttt ggcagtagca ataggtacgg cacatggact atataaggga
    18001 gagccaaagc ttgatttaga tagattatct gaaattagag aggttgtatc agttccatta
    18061 gtattacatg gtgggtctgg aataccagat gaggttatta aggaatctat aaaaagaggt
    18121 atttgcaaag ttaattatgc aactgaatta aggattgctt atagcaatgg ggttaaggat
    18181 gtgcttaatt ctgacccaga ggtaattgac cctaagaaat atggtaaaaa aggtttagag
    18241 tcagttaaag attttgttaa gagtaggatg gaagtttgtg gatgtgtagg aaaagcaata
    18301 gggacagtga agtgttaata ataaagggaa agaataagta agattaaatt aaggggtgtc
    18361 tcaaaatgaa ctttttagtt catgaggcac tcttttttgt gtgaaatcaa atatattttc
    18421 atcattctga caataaaaaa gaagatta
//
LOCUS       DAEPOG010000068        18228 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEPOG010000068 DAEPOG010000000
VERSION     DAEPOG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.068"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            19..765
                     /locus_tag="KR432_001942"
     CDS             19..765
                     /locus_tag="KR432_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640479.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            765..2681
                     /locus_tag="KR432_001943"
     CDS             765..2681
                     /locus_tag="KR432_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640480.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            2960..3877
                     /locus_tag="KR432_001944"
     CDS             2960..3877
                     /locus_tag="KR432_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0640481.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            3955..4692
                     /gene="cas6"
                     /locus_tag="KR432_001945"
     CDS             3955..4692
                     /gene="cas6"
                     /locus_tag="KR432_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0640482.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            4698..6575
                     /gene="cas8a1"
                     /locus_tag="KR432_001946"
     CDS             4698..6575
                     /gene="cas8a1"
                     /locus_tag="KR432_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBH0640483.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            6577..7491
                     /gene="cas7i"
                     /locus_tag="KR432_001947"
     CDS             6577..7491
                     /gene="cas7i"
                     /locus_tag="KR432_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0640484.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            7493..8194
                     /gene="cas5b"
                     /locus_tag="KR432_001948"
     CDS             7493..8194
                     /gene="cas5b"
                     /locus_tag="KR432_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBH0640485.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            8205..10526
                     /locus_tag="KR432_001949"
     CDS             8205..10526
                     /locus_tag="KR432_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0640486.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            10541..11092
                     /gene="cas4"
                     /locus_tag="KR432_001950"
     CDS             10541..11092
                     /gene="cas4"
                     /locus_tag="KR432_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBH0640487.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            11095..12075
                     /gene="cas1b"
                     /locus_tag="KR432_001951"
     CDS             11095..12075
                     /gene="cas1b"
                     /locus_tag="KR432_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBH0640488.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            12078..12344
                     /gene="cas2"
                     /locus_tag="KR432_001952"
     CDS             12078..12344
                     /gene="cas2"
                     /locus_tag="KR432_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBH0640489.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   12528..14145
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12528..12556
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            14335..14763
                     /locus_tag="KR432_001953"
     CDS             14335..14763
                     /locus_tag="KR432_001953"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH0640490.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(15219..15998)
                     /locus_tag="KR432_001954"
     CDS             complement(15219..15998)
                     /locus_tag="KR432_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640491.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            16322..16867
                     /locus_tag="KR432_001955"
     CDS             16322..16867
                     /locus_tag="KR432_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBH0640492.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            16899..18035
                     /locus_tag="KR432_001956"
     CDS             16899..18035
                     /locus_tag="KR432_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBH0640493.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            18039..>18228
                     /locus_tag="KR432_001957"
     CDS             18039..>18228
                     /locus_tag="KR432_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH0640494.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLI"
BASE COUNT         7441 a         1703 c         2991 g         6093 t
ORIGIN      
        1 aggaaggata gttaaataat gaaaatttta actgtaaata atttaagtaa agtatatggt
       61 aaaaaaataa ttttcaacgc attaaatgac ataaatttta gtatagaaga tggagaattt
      121 gtaggaatta tgggtccatc tggtagtgga aaaactactc ttttaaatat gatttctact
      181 attgacaagc caactacagg aactatggaa ttaaagggaa agaatccttt attgttaaga
      241 ggtgaggaac ttgcattatt tagaagaaga gagttaggtt ttgtatttca ggattttaat
      301 ttattggata cattaactat aggagaaaat atagtattgc cattgacatt agataaggtc
      361 agtgtaaaag agcaagatga gagattgaat gaagtatcta caatattagg tataaaagat
      421 ttattaggaa aaagaacttt tgaagtatca ggtggtcaag ctcagaggac tgctattgct
      481 agagcattga ttaacaatcc atcaatactt ttagcagatg aaccaacagg taatttagat
      541 tcaaagtcat caaaggttgt aatggagcta tttcaaaaga taaataaaga aaataaagtt
      601 actactatga tggtaactca tgacccatta gcagcgagct actgtagtag aatcttattc
      661 ataaaagatg ggtctatata taatgaaata tataagggta gtagtagaga gcagttttat
      721 caagagataa tggatgtact tacattattg ggaggcgata actaatgaat ataaaacagt
      781 ttgctattaa caatatttca agaaatatta aagcgtattt aggatactta gcaagtattg
      841 ttatatcatc ttcactgcta ttttctttta ttatgtttac aagtcatcca gatttagatg
      901 tatctcaatt tcctgattat ttaaaagaag gtctcaagat gagtaagata attgcatatc
      961 tattcttatt cttctttgtg ttttattcag ttagtgtgtt tcttaaaagt agatataaag
     1021 aatttggaat actttatatt acaggtatat caaaacgcca agtaatgaaa ttaatcttta
     1081 tagaaaatat gataataaat atagtatctt ctgtaattgg aataataata ggcttaatct
     1141 ttgcaaagat atttttagtg gttatgtcta cttttttaga attatcgcca ttaaaattct
     1201 atataccatt taatgcgata ttgtctactt taatctattt tatgatttta gctgtattaa
     1261 catcaatatt tacttatttt atagttaaag aaaatcaggt gttgaagtta cttaaaggta
     1321 caaaaactcc aaaaccagag cctaaaacat cactaatgct tgctatatta agtatttgtt
     1381 tgtttataat agcgtattat aatgctatta cctcaaccga ccaatataca acatatctgc
     1441 gcttagttcc agttacatcc cttactatag tagcaactta cttgttcttt tctcaattta
     1501 gtgtattttt tatgaagaag ctaaaactaa acaagagatt ttatagaaaa aatacaaata
     1561 tgctttggat ttcgaacttg atttataagg taaaggataa tgctagaata tttttcttga
     1621 taactataac ttctgcagta gcatttactg ctataggtac agtttattcc ttttggaaag
     1681 atgtagagcg tcaaattaat ttaatatatc ctaacacaat atattattca acaatgacat
     1741 tgcataatga ccctaaaaag cctgatagca aagaaaaaga caaggaaaga atgatgttta
     1801 tagaaaataa tctaaaaaaa gagaatgtag actatacaag gactaatggt gaatttaaaa
     1861 ctgtatttcc caagaaaagt gattttaatg tcagaattat gaaagaatct aagtacttag
     1921 aaattacaga aactataggg gttaagccta taagttttga agataatgaa tgcatatcat
     1981 tattatcatc aagtttacca ggaaataata acgtcaaaga aaatattatt gtagggaata
     2041 gaggtttaaa ggtggcaaaa caagtggaag aatgtgttat gccagcatat tacaaaaata
     2101 tgtatgtggt aaaggataat ttttatgata gtatagaatc aaaatttata gtggatagat
     2161 ttactgcatt tgaagtagag gattcaagta aaactataga gatatgcaga gaatttgaaa
     2221 ataaatttaa taatgagtca gggatgcaac catatttgtt tttctctaaa gaattaatga
     2281 taggaactgg gaaattaata tattctacat ttatattttt agcaatattt ataggtttaa
     2341 ttttctttgt tactacaagt agttttttat ataataaatt ttatatggat tgccaagttg
     2401 ataagaaaaa gtatgagcaa ttaaataaat tggggatgac atataatgag attaaaaaag
     2461 cttcaacaat tgagattgga atagtatttt tgtttcctta tgtagtggct gttatacatt
     2521 caatatttgc ccttactgca ctgaaaaatt cttttgatat agaagtagca tcatcagctg
     2581 ttttggtaat gggaagcttg ttcttggttc aaatagttta ttttttaatt gtaagaaata
     2641 attatttaaa agagataagg ttaaatttag tcaattttta gttactatta ttaaatgata
     2701 ataaaaaggg tttaaaatta attaatatta ctggaattat agatgtttat tttttcagta
     2761 atatttttta ttgtattaac tattaaaatt ttgtacaaat ataaaatata atatggtaaa
     2821 tgtcaaaatt ataaaaaaat tctacttata taaaatataa agaggttata tgtacatatg
     2881 tgtagaaata aataataaaa atatcaatta atgttattag ggggggttac tcattagtaa
     2941 attagctaat acacttagaa tgatgatatt aattcaaagc agaggcaaaa tgaaatgcaa
     3001 agaattatca gaagagttgg aagtaagtga aaggcaaata aaaagttaca agatgtattt
     3061 agagcaagct ggtatattta taaattcaac acctgggatt tatggtggtt atgaaataga
     3121 taaatgtaat agcatatcat taattaaatt attagatagt gaagtttcaa ttctagatat
     3181 gataaattca cagttagagt ataataatga tatttacaaa aatgaattta ataatattgt
     3241 agaaaaaata aaagcagtat taaatacagg agaaaaaagc gatacatata tggattattt
     3301 tacagttcaa gcacaacgta attgtgatta taagtctgaa aaaaataagt gtaatgaaat
     3361 aattagagca tataccacaa aacacaaatt ttggatagaa tattattcat taaattcagg
     3421 aaatagtgaa agaattgttc atccatatgg tttatttaat tataaatcag atacttatat
     3481 ggtagctttt tgtgaaaaaa gatttaagtt tatagacttt aaattatgta gaattaaaga
     3541 ttataaggtt ttagaagaaa aatataatgt agataaaagt tttagttggg atgaatatag
     3601 taaaaatagt ataggaattt ataaaggaga agaaattaat gttgttataa aaattagtca
     3661 tccattttca ataattataa aggaaaaagt ttgggttaat aatcaacaaa tcatagaata
     3721 tgatgataaa tctataatgt ttaaagctaa aatgagaggt tatgaggaga taaaaagttg
     3781 gattttaagt atgggagctt atgttgaggt tgtagaacca gatagactta gaaatgatat
     3841 attgtcagag attgaaaaaa tgaaaaaaat ttactgattt tttcaaataa tttatacttg
     3901 gaccaatata cttcacggtc tagtgttaat atattaattg agagaggtga tattatgaaa
     3961 atgaaaattg aatttagtac aggttgtata ccaatatctt ataattcgct ctttatgagt
     4021 attataaaag aagcaataag aaaatctaat gaagattact ataaaaacat gtactattat
     4081 aaagaaaaga ataataaaaa aactaaaaat tttacatttt cggtttatat aaaaaagtat
     4141 agtattgaag gtgacaattt tattatagaa gataaagtaa tattaaatat aagtactcca
     4201 gatttagaat tgggttttca tatatacaat gggttaatga cttctaaaaa atgtttatat
     4261 aaagcctatg aacttacaag aattagaatc gatttatcaa gagaaaagaa ggtaacagaa
     4321 gaaagggtac tatttaatgc attgtctcca atctgtgtaa agtctaaaga aggaaaattc
     4381 ttggagataa ctgatgatag atacatagaa gaactaaact atataactaa tgaagtggtt
     4441 aaaaattata ggggaaatgg attaaaaaga aagttagaat ttgaaaatat aggtctcaaa
     4501 aagatagtag ttaaagagag cttaagagaa tttaaaaaaa taacaggtaa agagtatcaa
     4561 tatattaatg gttacaaagg taaatttata ttgaaaggtg atattgatga tttaaatctt
     4621 atctacaatc taggaattgg ttttagacgt gctcagggat ttggagatgt agatatttta
     4681 gaatggaggt gagataaatg aagttaagag tttatagagg agattggttt tttaatatgg
     4741 gtatagttgg atttttaaat ataataaaga aagcagataa acaagctgaa atatttatta
     4801 tggaagatta tatcgaattt gatagcttat ttcttgaaaa ctttcatgag tattatttta
     4861 attactttat ggatgaatat gatgtatcaa aaagaattaa aaaaaatatt gattacagta
     4921 taaattttat taaatctaag cctgatagaa tcaaagatgg aattaagaaa attaaagata
     4981 gtgtgaaaca acaaaatgat aagattaaaa aatttgatga agaaaaattt aacctgataa
     5041 aagagaagct agatagcatg tctaagataa aaagttatga tgaatttgac agtttagaag
     5101 ctttagtaga tgagactata gatatattta aaataaaatc aataaatgac aggcttacag
     5161 caaatcttta taaatatgta gttcaagata attattttgg acaagttagt ttttttaatg
     5221 ttgctaaggc gaaattagat ttagatggat taaaacagat aatgtttaat gattatttaa
     5281 gacaaattat atattttgga gagttagcag atttattaga agagaatgac tatgataagt
     5341 tgaaaaacta tttaaatgat agattaaata gtatagctaa ggatataaat gaaaaaaaag
     5401 ttagtaagtc tagtataaat actatagaaa aaattatgaa ggaaataaat aaaaacttta
     5461 taaagaaaaa taaaagcata gaagatataa aagagtatat ggatagttta gaagtatgtg
     5521 aaatgtgtgg tttatataag ggaatattag atgaatactc agaaagtaat tttgctccac
     5581 ttggagtaag caccaataat gctaggaata tgttttggaa tcaggcgtat gtaccatcaa
     5641 tatgtgatat ttgtaagcta atattgttct gtactccagc aggggctact tatacaagaa
     5701 agaattattt aattaatgaa gaaaatgagt tttatttatt tgttaacatg gatacttcta
     5761 taaatgagct ttttgaaaga aataatagtt taaaagctca aaaaagtgaa tattcagatt
     5821 ccaaagatga aaatcctttt aatcagctta taaaaagtat agtagaagaa aatacattaa
     5881 aaagtgaatg gcaacttcgt aatatacttt ttgtggaatt taaagcaagt atagattcta
     5941 aaaaatgtaa aataaattat tttaatattc caacttattt agcaaaattt tttacagaca
     6001 agtatgcaaa taaaaaaata caaagtatct atgattacaa gcttaaatca aatgtactag
     6061 atttactctt gaaaaatagg gacttgaagc atctaactaa taacatttta agaaataaag
     6121 ttaaaaatga tatggaaagc aacaacaaat caaatatatc aggaatagat tgttttagag
     6181 tagtccaatt aagagcatta ataaatagct acaaaaaggg ggtatataaa atggatagta
     6241 aaaatttgga gaagaatgat gaaaagctga gaataattta ttatttaggt tgtgatatac
     6301 atgactattt tgttaataaa aattctaaaa ataaaataga tggagtaagc tataaacttt
     6361 taaattcagt taaagtcgga aataaaagtg attttatgga tacaattata agagttttta
     6421 tgtcagctga aaaacagatt ccagcattta ttttagatat agaaattgaa aaagatttag
     6481 actttgaatc tataggtcat gcttttatat ctggattaat ttctggaaaa tatgaaggaa
     6541 aagacaaact accaaacgaa aaggaggaaa aataagatga aaagagggtt aacatttaca
     6601 gtaatagcac aggctcaaag tttaaactat ggggaaggga taggtaatat atcagaatta
     6661 aaaaaattaa gtagagatga tggaaatata tatacatttg catctcgcca gtgtttaaga
     6721 tatgacatag tacgattagc agcagaattg tttaattgga atttacaggt tgtcgataaa
     6781 gaaaaaggta ctattcaatt taaagacaat ataagtataa gagaatcaga agaaatggat
     6841 ttatttggat atatgaaaac aaataaaaaa gatgagaaag ataaaaaagg aggttctttg
     6901 actagagaag ctactgtaag attaagtaat gcaatttccc ttgagccata tagaagtgac
     6961 atggactttt taaataataa aggattagca gatagaatag gagaacatcc taatcttgct
     7021 aacatagagc aacatttaag ttattataca tatacagtga ctatagattt atctaaaata
     7081 ggtaaggatg gagatataga acttgataat aaagagaaat gtagaagagt agtagaattt
     7141 ttagaaataa taaaagtatt gaatagaaat attagaggta gacaagaaaa tttatctcct
     7201 ctatttgtag taggaggagt atatgaaata gcaaatccat ttttcttggg tagaattaaa
     7261 ttaaaaggag ataaaaatgg atttaaaata aataagcaag ctatagaaga agtaattcaa
     7321 ggtacttttt taggaaaaga tttaaaagaa tttacctatg ttggaatggt agatggggta
     7381 tttataaata aagaagaatt taagggattg tttgaagata attttttaag tgtagataaa
     7441 ttttttggac aactagttaa agaagtaaag gaatattatg gggtgaatta atatgaaggt
     7501 tttgaaatta gatttatttc aagaaactgc atgctataaa aaaccatttg ctatgaagat
     7561 tacagagaca tatccactgc ctccatattc tacaataaat ggattaattc acaaaatatt
     7621 aaatgctaca gaatatatac catttaatat aagtgtacag ggaacatatg aaagtatatt
     7681 taataattac cagacaacat atttttataa aaaagatagt attacatcta tgccaatgaa
     7741 tagtcatatg ctattgaatg taaatttgat aattcatata ggtggggatt ttgatttatt
     7801 ggaagaaata tatgatagtt tattaaatta tgacgagcac ttatcattag gtagaaaaga
     7861 agatttagta agaattaatg atattagatt tgtagaagtc aataaatttg aagtagatga
     7921 ggatatacaa ttagatgatt atgaaaatta taagttatca gaatgtaata taagaatgcc
     7981 tatatatata ccaaaaacaa gtttacaaga tacagatata gatggtataa gttacagact
     8041 caataattac tatgataata atgctgtgga agataaaaaa agggtttgga ataaggtaga
     8101 tagttattat gttgaggatg gaaatactat atcaattgga gatatattat tagataatga
     8161 aggagattta ttatatttta ataatataaa ttaggaggga gagtatgtta tatgctaaat
     8221 ctaatccagt ggaaacactt agagaacata ctgatgaact tttaaaacaa atgaatgtat
     8281 taagagaaag ttatggaaaa aatataaata gcttagattt tctagaggaa gagatatttt
     8341 ggcaattact tgactttgtt atagaatttc atgatattgg aaaagcattt tcaccgtttc
     8401 aagagttaat aaaaagcaga atggatacta aaataaatga acctaaaata gtaactcatt
     8461 tagaaaataa tgtaggccat aattatcttt ctcctgcatt tattgattat agttatatag
     8521 acaggaaaaa aaataaagaa cttagagcag tattaaatca agttattgta tatcatcatg
     8581 aaagagatat ctttatagat aaagatttta aaaatttaat acaaaaaata ttagatgaag
     8641 atttaataaa taaagtttat gaactacagc gtgaatttaa agttagatat ccaataaaaa
     8701 cagaaaaatt gagtaaagta tatctacaaa gtgtagaaaa aagaatagat aaaaatcaca
     8761 agtactataa tttatatatt atgttaaaag gaatactaca tagactagac catagtgctt
     8821 ctgctcatga agttgtggaa tgcaataatg ttataaatat aggagaacag acagaaaatt
     8881 atttaatgaa agagtttggt agtttgagag aggctcaatc atttgctaaa tctaatagaa
     8941 ataagaatat aatactgatt gcatctacag gaatgggaaa aacagaaact gctttgattt
     9001 ggatagataa ggataaagca ttttttacac taccattaag agtaagtata aatgcattat
     9061 ttgatagggc taaaaatata attggagtag gagaagcaaa tgatacattc ttaggtctgt
     9121 tacattcaac agcaatagat tatttagaag aaagtaatca ggaaaattca agtgagattg
     9181 tagatttagc aaagttatta tcttgtaaac tgacattttc cacaatagac caaatattta
     9241 aatttccttt tttgtataga ggctatgaaa aagtgtactc aacattagca tattcaaaag
     9301 tagtaataga tgagatacaa gcatattcac cagaaatagc agctgtattg gtaaaaggta
     9361 tagaaatgat acataagata ggtggcagat ttatgataat gacagctact atgccaacca
     9421 tatatataga tgaattaaaa aaaagaggtg taatgaatag taacttagct gacttgacat
     9481 gtaacacaga aaaaataaga cattgtgtgt ctattgtaga aaattcaata gatgaaaact
     9541 taggtaaaat aatacaaagt ggaatgaata aaaaagttct tgtaatagta aatacagtta
     9601 aaagtgctgt agaaaaatat gagttaatag aagaaatagt taaaccaaaa ggaatagatg
     9661 tcaacttaaa tcttttacat tctatgtata tacaagaaga tagagcaaaa ttagaaaaat
     9721 atataaaaga atttgcagat agtgatagta atggtatttg gattactaca caattagtgg
     9781 aagcatccct tgatattgat tttgatgagc ttcatacaga aaattcaaca cttgacagtc
     9841 tatttcaacg atttggtaga tgttatagaa gtcgtgagta tgaagaaaat agcccaaata
     9901 tatttatata tacagaaaaa gctacaggaa taggtagtat ttatgataag gatattgtag
     9961 aaaaaggact tgagctactg aaaactttta taaatggaaa agaatgtgtg aagatgaaag
    10021 aaaaatataa ggtagaaatg gttaagatac tatactctaa agaaagtcta gaaggtacag
    10081 cttttgaaaa gagatttacc agtgctttaa atattttaga tactataacg ccatatagtt
    10141 taggttctaa agatgcacaa aatatactta gaaatattga aggatataca gtaattccag
    10201 aggatatata taataaaatt gaagaaactt taatcaaaga ttatgaagag ttgggacaag
    10261 agcttataaa tgcatatagt aatgatgata gacaattgat aaataaaata aagtctaaga
    10321 gaaaaaaagc aagaagagag ataattaaaa aaactgtaaa tttaccaata tataaagcta
    10381 aacaaaatgt aattgacata caggtgaaag gtcttgaaga tttaaaaata cttttatata
    10441 aatatgatat acacgaaaat aagcatacaa aacagatatt tggtaaaggt gtgcttataa
    10501 gtaatgaaat agaccaattt atttgatttt tatattaaca atgtggctaa aatttactaa
    10561 agaaggtgat tttgtgccaa tagatttaga agtattaaaa gttcaaggcg taaaaataaa
    10621 ttattattat gtatgtaaaa gaaaactatg gctattttct aaaggaataa ctatggaaca
    10681 aaatagtagt agagtattgt ctggaaagat agtacatgaa gattcatata aaagaatgaa
    10741 aaataaagaa atgttgattg atgatatctt gaagctagat ataataaagg acgagtatgt
    10801 aaaagaagta aaaataagtt caaagatgaa agaagcagat gaaatgcagt tatactatta
    10861 cttatactat ttaaagaata gtatgggtat agagaaaaaa ggaactataa attatgttaa
    10921 ggaaaagaaa caagaagaat tgatattgac agaagaaaaa gaaaaaatta ttgaaaacac
    10981 acttataaat ataaatgaaa taaatatgca gttatctcct cccaaaattg agaaaaaaca
    11041 ttattgtaca aaatgtgctt attatgaatt ttgttttgtg aaggaggagt aacaatgtat
    11101 aaagactatt atattataag taatggaaat ataaaaagga aagaaaatac gatttactat
    11161 tacaatttag aaggtgagaa aaaaatatta ccagtagaac aagttgatga tattcatata
    11221 tatggagaag tagatttaaa cacaaaactt ataaattaca taagtaaata tggggtgatg
    11281 cttcatttct acaattacta tggatattat tcaggtacat attatccaag gaagaaaaat
    11341 gtatcaggat tcagtttagt atgtcaatca gcctgctatt tagataaaaa tgaaagatta
    11401 tacttagcaa aatcctttgt ggaaagtgca tcataccata ttttaagaaa tatgagatat
    11461 tacaaagttg atgaaagttt aattgatact ataaaggatg aactaaatga tttgaatgat
    11521 gtcaaagaaa tatcagaact tatgggtaaa gaaggcagaa taagaaaaac atactataaa
    11581 tcatttaatc aaatattaaa agatgatttt gaattttata aaagagaaaa aagacctccc
    11641 aaagacccag taaatactct tatgtctttt ggaaatagca taatgtatac gaatgtttta
    11701 agtgagatat ataagacaca acttgaccct acaataagct ttttacatga accatcaaca
    11761 aagagatttt ctttaagttt agatattgca gaaattttta agccaattat agttgacaca
    11821 ataattttta gcctgattaa taataaaaga attaataaga atgattttga atatcaagac
    11881 aaaatttgtt atttaagtga aaaaggaaag aagaaatttt tgcaagaatt tgaaaataag
    11941 atgcagacaa ctataaaaca tagaaatcta aatagaaaag tttcatatag aatgtttata
    12001 aggttagagt gttataaatt aattaaacat tttataaatg atgaaaggta caaagtttta
    12061 aaggtgtggt ggtaagtatg tttgttattg ttacttatga tattgttgaa gcaaggtcgt
    12121 taaatagaat tagaaggata cttagaaaat atttgacttg gacgcaaaat tctgtttttg
    12181 aaggcaatat tactgatgga aagttacata aatgtatttc tgaaatagaa aatattattg
    12241 ataatagcga ggattcaatc tatgtttatg agataaaaaa tcctaattca attaaaaaga
    12301 aatgttatgg gattgataag tattctgatg aaatgtttat ataggtttgc agtgagcgat
    12361 atttatgcta aaataggtgt taacagttgg aatataaggg attgaaggtg tatgataact
    12421 gttatcaatt gcactactgc tcgctcactg caaattttga tgtttttatt gaattataat
    12481 tgcttgattg aagtattttc aatgtattca attataccta ttttggggtt ttatattaac
    12541 taagtggtat gtaaataagc aactatagta gaagcaaaag cgacaacaac taaaggtttt
    12601 atattaacta agtggtatgt aaatttggtt aacttaagtg ctatgaaagg aattaatgaa
    12661 gttttatatt aactaagtgg tatgtaaatt taaaggtgca agtcttaata cttgttacat
    12721 gagttgtttt atattaacta agtggtatgt aaataacaaa gaaccatttg aatcacgaaa
    12781 actaaaaaac agttttatat taactaagtg gtatgtaaat ttattaacct tatcagaaac
    12841 ctgaccatcc caattatagt tttatattaa ctaagtggta tgtaaattcc atttgtctaa
    12901 tgcttctaac tcttttttta tacgttttat attaactaag tggtatgtaa attccaaaat
    12961 gggcttggag ttaagagttg tataagaaac gttttatatt aactaagtgg tatgtaaatt
    13021 aattacacac tcccttttaa cgaacatatt ttagattgtt ttatattaac taagtggtat
    13081 gtaaatagac tcgacataga atcggttact aaatccgagg gtttcaggtt ttatattaac
    13141 taagtggtat gtaaatacta aacctttttg tttactttta tttctcattt tacgttttat
    13201 attaactaag tggtatgtaa atccggattt ttctgcttcg actgttgtta ttgagacttg
    13261 ttttatatta actaagtggt atgtaaatag taattccatg caatctttta tattgctttt
    13321 taacctgttt tatattaact aagtggtatg taaatagata tacaaggtga aagtgttgaa
    13381 atttcaatct tgttttatat taactaagtg gtatgtaaat attcttagat gttataattt
    13441 gtgttgcatt taaatcgttt tatattaact aagtggtatg taaatacaat attattgttc
    13501 atttcattta ataattctag tagttttata ttaactaagt ggtatgtaaa tttgtttata
    13561 cctttaatct ttaaaattaa atataaaagt tttatattaa ctaagtggta tgtaaatcat
    13621 tgtatactaa gcttgatata gtgcaaaatg aaacgtttta tattaactaa gtggtatgta
    13681 aatactaatt gggttaaaca aatggatata actaaattag ttttatatta actaagtggt
    13741 atgtaaatcc aacccttcgc acgaatccgt gcgacgaaat ataaagtttt atattaacta
    13801 agtggtatgt aaattgtaaa aatattgctt agcatattga tatcactaac tgttttatat
    13861 taactaagtg gtatgtaaat acaagaggtt tttcagcaat atatgtactt ctataatgtt
    13921 ttatattaac taagtggtat gtaaattcat caatattaat tttgggaata tcaacatttt
    13981 ttgcgtttta tattaactaa gtggtatgta aatgcgataa gagattcgag tctttttact
    14041 gggcttaatc gttttatatt aactaagtgg tatgtaaata ggataaagaa aagactcaca
    14101 caagacacag tgtcaggttt tatatcaact aagtggtaga taagtagaat attagcaata
    14161 tcaacgagta tttagaaact tattttatat taataaagca gtatataaaa ttgtagaata
    14221 aacaatagca tatactaaaa catccacctt atattaacta aatcatatct aaataaaatg
    14281 cacttaccta taaacattat aaaatcaata caaaaatgag gtgaaacaaa atttatgata
    14341 aagaaattaa acaataaaga cataaataaa atcatggaaa tatgggaaaa aagtacaatc
    14401 aaagcacatg actttataag taaagaatac tggcaaaata actacaatac tgttaaaaac
    14461 gaatatatac ctatatcaga tacatttgta tatgatgatg gagatgaaat aaaaggattt
    14521 ataagcataa tagataaaag ctttatagga gctttattta tagaacccaa ataccaaaat
    14581 ctaggtatcg gaggtaaact tttagattat gcaactaaaa aatataaaag tctaagttta
    14641 gcagtatata aagataataa aaaagcagtt gtgttttata ataaaaaagg ttttaatata
    14701 gtaaaagaac aagtaaatga agattcagga tttaaagagt acataatgga atatagtaaa
    14761 taatatgatt acattctatt aaaagaataa ttaatttatt ttctatttga tttttataag
    14821 agtaattttt aaaatagact atatgaagat aaagatattt attatagatg taagttaatc
    14881 agaattgata tagctacaaa agaggaaaat aaaattgtat ggtgtatttc agatttaaca
    14941 aatttactat ttaaagataa taagtttgat ataatattaa agatactttc acctataaac
    15001 tatagggtat ttgaaagaat tttgaataat tatggaatgc taataaaggt ttgttaaaat
    15061 ttaataatta ttagattata gaaaattgcc atgaaattag ttcagcaaga actagtttca
    15121 tagcaatttt aattcttata agtgtattta tttttacaga tttaaaaaac ttatacagta
    15181 tctaataatg taatcttatt actttagcct ataaatattt aaatcttaaa atgttctctt
    15241 tgagataagt ttatttcaga gtcacaattt ccacaacagt aagatgttga gacatcttta
    15301 aatttttcag tattctcatc aaagttcatt tttttctttg caataattcc taccttagtt
    15361 aaattagaac cacaattatt acagttttga attttacata ggttatatag tttattaagt
    15421 gtaatatctt ctaataattc atcacaatta caacatctga tagaagaaac ttcactagac
    15481 atttcaatag gttcagcttc tttagaagaa aatttatatg ttatgtcatt tgatacttta
    15541 taacctgtat caaatatatt agaattacaa cttggacata agtactttgg aagatatata
    15601 tctttttttt caaaagtatc actactaata ttacagttta attcatctaa aattggtctt
    15661 tcatctaggt tattaccttt atttaagctt agaagataat tataaatttt tttatcttca
    15721 ttctgttttg atgtatctaa caaatgctcc attatattag tgtatttttt gttttttgaa
    15781 ggcatgttta aatccagata attgttttca atagcaaagc atactatatt gaaatagtta
    15841 tatttaactg caaaaaataa taacatttca tcaaatactt tatctattat tttgtcagaa
    15901 atacttatcc aaaaatctgg tgactctaag taaactttcc tactaagttc atctaactct
    15961 tcatctatat tatttatatt attttcatta actttcatat catcacttcc ttttaaatat
    16021 cttgtgtata aactatttgt tactattttt tatataaaat aaaatcttat ataaaaatat
    16081 atcatattat aatttacaag tatagtctaa atagagctgt ttttaagcaa agttttataa
    16141 atgactatta aaatataaag taacagtata aagtttactt ataaattaaa cgtaaaccta
    16201 aatacttgta atggttgaca aaaaaacgtc tttattttat actaaaacaa agctttaata
    16261 gaggctttgt atatgtacat ataaactaaa tgaaataaga catatgaagg gaattataaa
    16321 aatgaaaaat actaaagtaa atattcaaga tttaactaaa atgtcaatat gtgtagcatt
    16381 attgtgtata tcatcttaca tatatatacc attgccattt actccagcag gagtaacagc
    16441 acaaacaata atgataaatt tgatagcttt gattttaaca ccaagacaag catttagtac
    16501 agttggtgta tatataatga taggtttaat tgggattcca gtatttggtg gaggtacatc
    16561 tggtattgga aagttgttaa gcccaacagg tggtttttat tttgggtttt tgtttgcagc
    16621 actaattatt agtttattaa aaggacgaaa taatgatatt aagagatata tactaataac
    16681 aatttttgtt ggaatgccta ttatatattt tatgggaaca gtatttatgt gctattttaa
    16741 tcaaatgaca gtagaagcag cactttttgc agcagtagta ccatttttaa ttggagatac
    16801 aataaagtcg gttattgcat cttttttagg cactaaactg aacaatgtac tcagaagaaa
    16861 tctatgataa atctataata taaggaaggg tgtaatgtat gaaggaagtt tttatattag
    16921 gaggattgag aagtcatata ggtctaaaaa atggaatttt tcaatttgtt caacctgaat
    16981 tactaggagc aagtgtttta aaagatttaa ttaaaaagta tgaaattgac aaaattgatg
    17041 agattatatg tggaaatgca gttgggacag gagggaatat agccagatta atgactttaa
    17101 cagcaggagt gtcaaatgaa gtacctgctt ttactgtgga tatgcagtgt gcttctgcta
    17161 tgatgagtat agacatagct ttttcaaagg ttaaatctgg tcagtgtaat ttaattattg
    17221 caggtggttt tgaaagtagt tctctacaac caatgcgcac atatcataag aatgataagc
    17281 gatataatac aaataatcca aattatacag ttgctcaatt ttctcctgat gataatagtc
    17341 aaaatagtat gctcgaaggg gcggaaagag tagcagagtt atatcaaatt gagagagcag
    17401 acttggattt ttgggtaaag gaaagtcata aaagagcaaa agaagcaaga gaagaaaaaa
    17461 tacttgagga tattatatct cctataaata atagtaccta tgatgaaggt atacgtgata
    17521 agatgagtca aaggttatta gataggatgc caccaatttt aggaaaagaa actattacaa
    17581 atgcagcaaa ttcatgtctt attaatgatg gagcttcatt tattataata tgttctaaga
    17641 agtatttaga acatgtaaaa aagaagccta aaacaaaaat tattaataca tgcacaatag
    17701 gaacagatgc aaccttaagt ccaacatcag cgatacaagc aatggataaa cttttagaga
    17761 tagaaagcct taattattta gatgtatctg ctgttgaatt taatgaagca tttgcagtta
    17821 tagatgtgtt gtttcaaaga aagtatccag agttaataga gagatataat atatttggtg
    17881 gagctttagc gtatggacat ccatatggtg cttcaggtgc aattattaca ttacatcttt
    17941 taaaagcatt agaaaaaact aagggtagat atggaatttg ctctatagct gcagctggtg
    18001 gtctaggttc agctttactg cttgaaaggg tgtagattat gaactattat gaacttttaa
    18061 gaataaaaaa agagaggcat aaaaataaaa attttttaat tatagatgga gaaaaatata
    18121 cttatgaaaa tatattaagt tatagtgagc aactaggaaa acaaatttct ttaggtgaaa
    18181 atatctcaat tttaatatat tctaaaaata taatgtttca gttgatta
//
LOCUS       DAEPOG010000069        17957 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEPOG010000069 DAEPOG010000000
VERSION     DAEPOG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.069"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(235..618)
                     /locus_tag="KR432_001958"
                     /pseudo
     CDS             complement(235..618)
                     /locus_tag="KR432_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      845..942
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            997..1173
                     /locus_tag="KR432_001959"
     CDS             997..1173
                     /locus_tag="KR432_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640495.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1399..1599
                     /locus_tag="KR432_001960"
     CDS             1399..1599
                     /locus_tag="KR432_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0640496.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1763..2062)
                     /locus_tag="KR432_001961"
     CDS             complement(1763..2062)
                     /locus_tag="KR432_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640497.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2284..2466
                     /locus_tag="KR432_001962"
     CDS             2284..2466
                     /locus_tag="KR432_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH0640498.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2537..3382
                     /locus_tag="KR432_001963"
     CDS             2537..3382
                     /locus_tag="KR432_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640499.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3606..3770)
                     /locus_tag="KR432_001964"
     CDS             complement(3606..3770)
                     /locus_tag="KR432_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640500.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4312..4644)
                     /locus_tag="KR432_001965"
     CDS             complement(4312..4644)
                     /locus_tag="KR432_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH0640501.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4888..5529)
                     /locus_tag="KR432_001966"
     CDS             complement(4888..5529)
                     /locus_tag="KR432_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBH0640502.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5510..5803)
                     /locus_tag="KR432_001967"
     CDS             complement(5510..5803)
                     /locus_tag="KR432_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0640503.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            6197..6256
                     /locus_tag="KR432_001968"
     CDS             6197..6256
                     /locus_tag="KR432_001968"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640504.1"
                     /translation="MIEYIYYYNNKRIKKINLA"
     gene            complement(6772..7440)
                     /locus_tag="KR432_001969"
     CDS             complement(6772..7440)
                     /locus_tag="KR432_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0640505.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(7385..7525)
                     /locus_tag="KR432_001970"
     CDS             complement(7385..7525)
                     /locus_tag="KR432_001970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640506.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(7584..9239)
                     /locus_tag="KR432_001971"
     CDS             complement(7584..9239)
                     /locus_tag="KR432_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640507.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(9468..9836)
                     /locus_tag="KR432_001972"
     CDS             complement(9468..9836)
                     /locus_tag="KR432_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0640508.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            9985..10779
                     /locus_tag="KR432_001973"
     CDS             9985..10779
                     /locus_tag="KR432_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBH0640509.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(10932..11360)
                     /locus_tag="KR432_001974"
     CDS             complement(10932..11360)
                     /locus_tag="KR432_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0640510.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(11836..12774)
                     /locus_tag="KR432_001975"
     CDS             complement(11836..12774)
                     /locus_tag="KR432_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0640511.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(13118..14773)
                     /gene="ilvD"
                     /locus_tag="KR432_001976"
     CDS             complement(13118..14773)
                     /gene="ilvD"
                     /locus_tag="KR432_001976"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH0640512.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(15097..15396)
                     /locus_tag="KR432_001977"
     CDS             complement(15097..15396)
                     /locus_tag="KR432_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640513.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            15656..16336
                     /locus_tag="KR432_001978"
     CDS             15656..16336
                     /locus_tag="KR432_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0640514.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            complement(16446..16703)
                     /locus_tag="KR432_001979"
     CDS             complement(16446..16703)
                     /locus_tag="KR432_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640515.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            17252..17623
                     /locus_tag="KR432_001980"
     CDS             17252..17623
                     /locus_tag="KR432_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640516.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            complement(17620..17760)
                     /locus_tag="KR432_001981"
     CDS             complement(17620..17760)
                     /locus_tag="KR432_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640517.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
BASE COUNT         6360 a         2699 c         1866 g         7032 t
ORIGIN      
        1 tctttatagt caatataaaa gacagtaatt tatctatagt gttacttata ttttatatat
       61 atggctattt ttcaacattt tacaatatat ataacattta tttttttaac ttttgtcagt
      121 aaaatgtcag caagacactt tatccacaat ttttatataa gttttccaca acttatcccc
      181 agtattatcc acataaaaaa ggtagctctt atattattag ctacctttta tttattaatc
      241 ttcatatttc cagcttttta tccaacttcc tatttctatt attttttctt cagttatttc
      301 ttctccatca tgtaattttg aaattaattc tgataaagga ttgttatgta aacctctaaa
      361 aactcttttt ccctctattt tgagatattc tctctctttt attaatactg tatagtgtac
      421 atgcctccca attctctatg atattaaaaa accttttttg gttaatctag ctaaaagtat
      481 aattgtagtt gtatgcttcc aaccatatgt tttttctatt tcatctgcaa catcttttga
      541 tattacagtt ttataaccag tattccatat gcatttcata atttttaatt ctgtatctgg
      601 taatttttta tttagcataa tatattcctt ctttcaattt atgtcacttc ttcaaattta
      661 tctagcttaa attataatag gagtttttta gaacaaaaac tatagttaat ttttaccagg
      721 tactctacgt gtttagtctg ttttttaagt gtaaaataat gttcatatct ataaatttat
      781 ttaactttca aacaatcctg ttgaataata actaattcta ttgtattatt ttagttgtag
      841 aataaaacta aatcggcaaa actagagaaa tttagtgacg caaagctata gggactaaga
      901 cttatataaa tatcttatga gttatgtcag ccagttgcca aagagatagt gttctttttg
      961 tttttatgaa agttttttag ggggataata atttatatgt ttggaaataa aatggataaa
     1021 tgtacacatg tattgactgc ttatattagc ggttcgtatg attattgtaa ttttttagat
     1081 acacaattag atgattttat attagagtac ggagaaagtg tagtagagtc ttgtttgcac
     1141 caagtgatgg tattggtaag taagtataat tagatagaca ttgatttcta gagaaccctt
     1201 tttagtttta tctacattta tattaatata tagcaatgta aaacgcttaa atatattttt
     1261 aatattaaga ataactaaaa tgttgactaa aaaatataat taagtagaca tttctattaa
     1321 tctatgatac aatagtattt gtggtaatac acaattaaat caagtatatc ttgatatagg
     1381 ttaataggag tatttttaat gaataatgga atagtgaaat ggtttaataa tgaaaaagga
     1441 ttcggtttta tatcaatgga aggtagagat gatgtgtttg ctcatttctc agctatacaa
     1501 acttcaggat ttaaatcatt agaagaaggt caacaagtaa gctttgatat agttaaaggt
     1561 gctagaggtc ctcaagcaga aaatataact atattataat atatagattt ttcgggaaag
     1621 actcttttta gagtcttttt ttattgttag tatatgtaag ataatcattg gtgaagtaaa
     1681 taaggtatat tgagtaatgt tcttgatagt ttatatgcta atgaatttca aaaaaataag
     1741 cactcctata aaaagagtgc tcttaatgta aatataagcc ttcattggtc cagcctaata
     1801 ccccgtattc tccaagttct aactcttttt tatatacatt accactttca gccatatcta
     1861 acacaatcat ttcttcttta gaatcaattt gtaaaatatg aggactgtgt gttgtgacaa
     1921 ttatttgtgt ttttaggaga attgctttta atgtaataaa aagtttacat acaacatagt
     1981 taatataaaa caaaattatt tttgagaatg agctaaataa aatcttaact ttacatacca
     2041 catagttgat ataaaacccc aaaataaact tagcatctcc aatgtttaca catatacaat
     2101 gctcttaaaa ttgcagtgaa catgcaatag tgcaattgat aatacttatc atatattctc
     2161 aataccttat atttcaactg ctaaacctca atttgtcaca aatatcgctc actgcaaaat
     2221 ttctatattt ttattatatc atagcatata attaatattt tcaattaatt tttacattta
     2281 attatgttta aattctataa taatgcaact ttaatgtttc tttctggaac taatataaaa
     2341 actatttcag aaagattagg tcatgcagat ataaaaataa ctatgaatag ctactctcag
     2401 tttttagaag agatggacag agaagcattt gaaaatttga gcaaaatact atttattgta
     2461 ttttaaagaa gatgacttat tcaatatttg atgctagaat ataattaata aaaatttaat
     2521 tataggtgat tgtattatga acattattaa aagcataact ttactcttaa cattattaat
     2581 tgtgatatta atgacaggat gttccccaag agaaagtgct agtgaagttg caaaaaaata
     2641 tattgataag atgaactctg gtgactatga aacagcatat agtttattaa gtaaagatag
     2701 taaaaaaaaa attagtatag aaaatcttga agaatttcaa aatattttag ttagtacaaa
     2761 aagaataatt agttataaaa taggtaaaga aaaaatcata aaaaaatata aacatgatgg
     2821 aaaagaatat aaaaatgttg ttaaattaga agaaactttt aatataaaaa accttcttta
     2881 taaaaaagat gattctctaa aagtaaatag atattttgta gttgaaaata acaaatacaa
     2941 gctattattg tatgaaagtt tcaaaaaaga atttggtatt aatagtatgg accttgctca
     3001 acttaagctt aatgattttg gactaaaata ctttaaagaa gttgacctta tactaaaaaa
     3061 agctattgct attaatccta ctgattcaaa gctttattat gcccaagcat gtaattatat
     3121 gaattttgat ggatttcctg gttattcaga tacaacatat aatgctttag attcaattaa
     3181 taattcttta aagtatttag ataaaaatga ttctgactac aaaaaaaatg caagtaatat
     3241 atataactta aaaggagtta ttcttatgtt tcacaaaaga tttgatgaag cacaacaata
     3301 tttaaataaa gccttatact ttaacaaaga taatgaagac ccaaaatata atctaaatac
     3361 aataaaaaaa catctaaact agatatttaa tttttgaatt tataaaatat ttttttatgt
     3421 ataattcagc actgaatcat aatctctact aaactaatta caagtttaaa ttataaagac
     3481 ttatttctat ataaatatta ctctataaca ataagtttct tatttaaatt ttaaaatatt
     3541 atctaaaaat cataaaaaag aaggtataaa gagctaccct caagctcaat ataccttata
     3601 aatatctaaa cttcattttt tactaatttt tctatatctt tgataatatg ttctttagat
     3661 ttttttccag ctatggcatc aaaaattgca acagcctttt tctctccaaa cttttctgct
     3721 acatcattaa agtctaaaaa tctaacactt tcttttggtt taccaaacat ataacacctc
     3781 tcatacattt ttatatattt attataactt ctatagactt atttttcacc aataaacgtt
     3841 agacaaatta gtccctttat ttttttaaac tagctttacc ttcaaaattc ttaacaaaaa
     3901 cagtttattc tactctataa ataaaaatag acaatttaat ttgtaggaaa ctgataatag
     3961 aatacagtaa ataaaacttt gtttaatcta tctgaaaaag ttatctatta aaactgctat
     4021 gaatttataa agaatattct agttcataaa aatacaaata tgtaaatata atcaccttaa
     4081 tattcaattt atttaaaata aaaaatgtat aaaagattta ataatgacaa tatactattc
     4141 tcaatacaat ctatgatgta aataatttaa aactactata ttatttaaaa atttatattt
     4201 atatatgcaa ttctaaaaac ttaataagaa gtgaacttag aaaataaagc ttaatttcaa
     4261 agacttaaat agaatattat tattagtcta aataaactac gtgtatcaaa attatatatt
     4321 aaattcttct aaaattttta ttgcaaatct tgcgtttgga tatgtaatag acccgccacc
     4381 aattacacct atttttatag cttctataat ttcatcttta cttgctccag aattaataca
     4441 accctcaata tgataacaaa tacactcatc acatcggctt aaaattgaaa tagctagtcc
     4501 cattaattcc ttattttttt tacttatagc cccttctgaa taagtcttat catcaagact
     4561 tccaaatact ctaaaaaact catctgtttc acttaatttt ttattgtaaa atcccctata
     4621 ttctttcatt tctctagtat tcatcttcta cctcctatgt ttttcttata agagtatcac
     4681 actgtactta tattttcaat attaaataca aattttcatt ttatgttcat tagcctaatt
     4741 tatattattt tttgaaatct caataagtta tcatataagt aagatttttt cagaactaaa
     4801 atagaaatct tagtaatttc tttattatct atctcagttc tgaaatatat tattcttatc
     4861 caaacattat tatagtgatt aattatatta gttattttta cctgaaattt tatacatcaa
     4921 ataagaatat ataaatggta gtattacaat tacaaagatt acaaagatat tataaaaagg
     4981 aatattaata aatgcattaa taattaataa tattccacca ataagccaaa tccatccagc
     5041 taaatggtgt gtcttattcc aattatcttc atcatcaaga gtccatggca aaattattcc
     5101 tactgagtaa ttttgtttac atttaggaag atagtttcca aataaaataa ctattattcc
     5161 taaaattaat cctgtataaa aattagactc caaactaact cctgttgaat ataaaaccat
     5221 cagtatttgt agaattactg aaagtatagc aactccccat tttgataaat tttgtataac
     5281 atttgaaaaa ttagcttttt taggctcatt ctcaagtcta aaccatgtat acaaatggat
     5341 acatagcatt aacaatggta caccaaaagc tgctataacc tttggagcat aattatctgg
     5401 atttccataa ttatcaaaat gaattgcaac ttcatttggt aatctattat aaaaatatat
     5461 tgatacaata aatggtataa aacatattaa agtagataaa ataaattgtt tatttatcgc
     5521 ttttttcatt atctttttct cccttctcca taaattgtga aaaccaaagc ataacctctt
     5581 cgaaaactga aatatttatt tcgtagaaaa taaagttctt atactttgtt tcaaatatga
     5641 gtcctgcctt ttttagttga gatagatgat aagaaattgt agcattcgtc atattaaatt
     5701 tttcactaat ttctcctgct gacattttat tctccctcaa cataaccaat atttctcttc
     5761 ttatagggtc tgaaagagct ttgaatgttt ctggaaaccc caacaccatc acctcgctat
     5821 ttagaatttc atctaaatag aatataacat aacatccatt ttatttcaat aactatttag
     5881 aaaaatttct aaatagttat ttccaaatat ttattgaaaa ttttcatatt aagattctca
     5941 atttacttaa gtcgctttta tataatctta ttttatctaa tttttttaat ttaacaaaag
     6001 tgtattgcac cacaattgtt aaagcaaaaa cctaatgact gatatactta tatatattac
     6061 atatactttt aatttcaaat taaaagtagc taaatccaat ttaaaaagta gttaagtcct
     6121 atttaaatag aaaagaatga aataagaaat atactgtata aaaatgcata aaaacctcta
     6181 ctaattagat acaactataa tagaatatat ttattattat aataacaaaa gaattaaaaa
     6241 aattaattta gcataacctt gttaaatata gatttgaata tgaatttaaa gttatgtaaa
     6301 attaaaaata caggaatgaa aattccattc ctgtataaaa tctaactttt gagtctcaat
     6361 acaatcttat tttgagacaa tcttttttgt tttatatttt cttatattta aaatataacg
     6421 catatcttat gcaacatatt catatttttc tttatattct ttcatcgcat cattccaagc
     6481 gtcttgtact gttacacctc ttttattagc aattttttgt gccagttcaa ttatcttcat
     6541 agctcttctt gatatgttca taaacattcc tcctacaaat taccatatat ttcatatata
     6601 taaatcaagg ttatattttt atgatatata atccttctat tgtaatatta caatttatat
     6661 gtgtattata tatgaactat attagaagat aattagaatt tttataaagc tatatgatta
     6721 gattaaatat aaaagcttta caattactta taaatttaaa aagtctataa actaaactaa
     6781 tgtttgatat accaatttta cgaccataaa taatgccatt actaaaaata ttggttttat
     6841 catctttgaa cctttcttta tagcaacttt agcacctaca taagctccca ttatcatcac
     6901 taatgcaaac actatagcaa ttttataatc tacttgacca tttatcatga ataataacaa
     6961 tgaagttata ttacttgtta agtttaatat ttttgtattt gcagaagcat gtaaaaaatc
     7021 atatccatat atttttataa atccaaaagt caaaaaagtt cctgtacctg gaccaaaaaa
     7081 accatcataa aaacccatta taagagccat aagcatccct agtcttaaat ttttcttatt
     7141 aacagcttca aaattatctt cttgacctaa atttttagaa ataaaggtgt aaatagccac
     7201 tattaaaatc ataaccacaa ccaaaacatt taatatctct tcacttattg ataatacaca
     7261 tctcacacct agaacactac caataatagt aaatggtact aattttttta aaagatcgtt
     7321 attaattttt ccagactttg catatctata ggcactactt atacatcctg ctgatgctgc
     7381 aaatttattt gtaccaatgg caagatgtac tggcactcca attgccataa gaacaggcat
     7441 gcttataagt ccctcctcct cctgctatag aatctacaaa agcagctaaa aaccccccta
     7501 tacataaaaa aataactgaa aacataaaat tcctccatca ttctctttca atttttctta
     7561 caatatatat tatataatct tatttaatac ttattatcca gcatattctt tctatattca
     7621 tttggagtta tactattaaa cttcttaaat atttgtgaaa aataactact gttattaaaa
     7681 cctactttct tacaaatatc actaatactc atgcttctat ctaacagata ttttttagat
     7741 tcttctatcc taagaagatt tatatactca gttattgaaa ctttatttct ttcattaaaa
     7801 atagaactca aataatttgg acttataaaa aaaacatctg atatactatt caaatttata
     7861 ttttctttat aatatttttt tatatattcg tttatatttt caattagatt tcctttatct
     7921 tttctccaaa atgctctatt cttatcaata cactctttgg aaaattcaaa taatacattc
     7981 cataaatctt catcactgtc tacaagtgat atttttttat tatatttatt tcttaaataa
     8041 gatataagtt ttaaatcctc taaatctcta agcactgttc tagacaatac aaatatcatt
     8101 tcctgaatag tatctctaac atcattatat cttaatttgt ttgtatctat tagattataa
     8161 acaccttcta aattcatatt tttaatttct ttttcaatat aaagttctaa ttcaaaggga
     8221 tattcttttg aagaattatt gaacatatct atcggattaa taatatactc ttcttctaat
     8281 aatctactat ttgctttttt aagtgcctct tcaagtctta tattttcttt tctactcttt
     8341 aatatctcct catcttttat actaagttga ttttcaaaaa aattcctctt agctatttcc
     8401 tgaataaatt cagacatcat taataaattt tcttttaata catataatct acttttagga
     8461 tttaatggaa attcattgta aattttgttt atgtatttta tatcaatacc agaactactt
     8521 gataattcag ataaattttt atctatatca atagacttat atactataaa attaccacat
     8581 tctatatatc caactatatt ttcttctaat actataggta tctctaaact ggaaacacca
     8641 tactcacaat ggtacacttt ttcaaagtta tcattattta tgcttttaac aagtttttta
     8701 gtacacggtt ttatattttt acaaatatcg caaaatttat tttctttctt agtctcaaat
     8761 attcctttat actttaagtc atatacagat attctaagct tcaatatatt agatatttga
     8821 tttgcaagat tttcaattat gtttacatta actaactctt gtatattgtt ttcagaaaat
     8881 actataggta tttgtctcaa actcgaaaat ttatgaggaa cataagtaat taatattact
     8941 aaatcaattt taccagtgtt tataatttca tgttctgaat atggtgggca ataaactata
     9001 gatgtttcct ttatattgac ctttttacca tcaatcattt gtattccttc tccctgaatc
     9061 acatataaaa gctgttcttc acttaaatga aaatgctttt cttggcagtt tcccggaaaa
     9121 aatcttacaa ttccagcact aagtctattg aattgtgttt ttgatggttc gtgtatccac
     9181 ataacctctc cccaatcaaa tttttgtact tcattatttg actcaataaa atccaccata
     9241 aactcacctc gtagttttta tctaaatatt tttttattcc tttaaatatc tccttataat
     9301 atacattatc ataattttct tttttattgt atagataaat agttatatag aacattgtcc
     9361 aattcttcct aaaaatatag tgtaaaaaat ctttcctcaa atgtttccaa ttgaatttac
     9421 tctttatatt agaaaaatca aaaaattatt tctttattct ataaggatta ttttttacac
     9481 atcttttttt cttttaagta atatattttc tcatctattt tatccataca actttctagt
     9541 tcatgtattt tatttttaat gttttgctta tgttgttgaa ataattggat tcttttatct
     9601 attgtaccat ctcccatatc acaaaaatga aagcattcat ggatatcttt aatagataaa
     9661 ccagtatttt ttaattcttt gataaatatt agacgctcta catcttcttc agagaaatag
     9721 cgaacaccac ttggttttct attgatagga agtaataaac ctttttcttc ataaaatcga
     9781 atagcagatg aagaaatttt tgttattttt gatacatcag ttatagaata tttcataatc
     9841 tctacctccc taaattttat atgaataaga atattataaa aataccttat acagtagtca
     9901 acaaaaaagt attgacttca agttaggttg aagttgtact ctttgaagtg taaaagaaaa
     9961 cttacaaaag aaaggatgat ataaatggaa aaatatgcaa ctatgattaa agaaaagcta
    10021 aatgaaggaa aggtcttatg tggagtagct gtttcactgt ctgattcagt tgtaagtgag
    10081 ttaatcggta tatctggata tgattttatt tggattgaag gagaacatag tgctttagac
    10141 cgtaaagaca ttcagcaaca tatgatagct gcccatgctg gtggtgctgc ttctttggta
    10201 cgattagtaa acagtgaccc tgaccttgtt aagcccataa tggatatggg aacagatatt
    10261 atttgtttcc catttattaa tacagtagaa gatgcacaaa aagccgtagc agcttgtact
    10321 tatcctccaa acggtataag aggatataat cctcaaagag cctcttatta tgggaatatg
    10381 aattattttg actatgtcaa aaacgcaaac aatgaaacat taaaaatgat gttaattgaa
    10441 cagaaagcag gttatgataa tcttgaagaa atagtaaaag tcgagggtat tgatattata
    10501 gcattaggcc ctggtgactt atctttagac atgggttttt ccggatatat gaatagaatt
    10561 gaaataaaag atatgatttc tcatgctgct gaaatatgca aaaaatataa gatgcccttt
    10621 attatttttc ctacaataga aaagaaaagt attgaagaat gggttaatat tggagcaaat
    10681 atgctttgtt ttgcacaaga tactaccttt atcacttatg ttatcaatca attattatct
    10741 ttttataatg agattgtacc aattcaaaaa caacattaaa ttataaatct aattttatat
    10801 taaaagagac taatatttta tctatctcta ttaaggatat tttcttatgt atcataatat
    10861 tagtctctct tattattata tactgctatc atcattagtt gctatttata ctatttttat
    10921 aatatacaaa tttattcagc taacgttttg cctaattcta tacactcatc ttttgcttct
    10981 tcatttgggt cttccattgc tgtaattcct tctccaccaa taattgtagc accagcattt
    11041 tgcatacgtt cttcccagtc tctcatccat tcacaatttc cccatccata tgaaccaaat
    11101 aatccaatct gtttacctga taccatagat tctaattctg aaacaaatgg ctccatatac
    11161 ccctcttcta gttgctctgc tcccatagat gggcaaccta atgcaaattt atcttcttct
    11221 tttaaatcac ttggtttcag taagctcaca tctaaaactt ctggtgtctt accttttaac
    11281 ttcacacctt cagccacaaa atttgccatt ttttcagtat ttcctgttcc actccaataa
    11341 acaatatata ttttactcat ttttacctcc tgtagtattt tttaattaat caatagttag
    11401 ttatagctaa ctaacatcta aaaaacaaaa gatttcttta tctgaaatct ctttattttt
    11461 ctaaatatag aatatcacaa ttgattttga aattcaatat ttattgataa tatttatcat
    11521 ttatatttta tattcatttt ataatatcaa aacactgtat cgtatttcta atctttcata
    11581 gaaaaatttc taacttatag tacttttgca tctcttataa atcttttata tgtataagta
    11641 catagaaaaa cacacctttt ccagatgtgc tatctataat atcgtatatt tatttttcaa
    11701 ttctaactag ctaatatgtt tataaatttt atcacttaat acctttatac attgatgtaa
    11761 aatcttacac ctaagtaatt tttattattt gtctcgactc tatataatat agctatttta
    11821 tcatcaaatc aaaaattatc ttttgctatt attcttgtat ttataaagat ttaaatataa
    11881 gtatccattt ccttgttccg ttctaggtat tccaagcgct ctcgtacatt ttattaattg
    11941 tgcatacagt tcagcttcat ctaaatctct ttcaaactcc tctcttgccc attctttaat
    12001 tagtgctaat gagcctgata cgtatggtgc actcatacta gtaccactca taatagcaag
    12061 tttattatcc atataagtcg atataatatt tcttcctgga gccaccaaat ctattgtagt
    12121 atttgaatta ctaaactttt caaccaaata gttttcattt attgcaccta cttctattac
    12181 ctcagcataa ctggctggat aactatactc acttgtacta gaatcaccat ctccattatt
    12241 acctgctgca cacactacag aaatattatt ttttactgct tgcatgacag cattttttaa
    12301 attcttatca tctttgtttc ccccaagaga catagatata atatcaacct tgttatttac
    12361 agcaaagtta atagcattaa ttatactttg atatgttccg gtaccatctt tatttaatgc
    12421 ttttgctatt aataatttac aatctggagc tactcccata acttcattat tatagttaga
    12481 tgcagctata atacccgcca catgagttcc atggccatta aaatcctcat atatattttt
    12541 atttccatta ctgtcatcac taaaatttgc accaccaatt atttttcctt ttaaaagagg
    12601 atgagatata tcacaacctg tatctattat accaactaca atatttttac cagtataacc
    12661 ttcatcccac attcccctag cattcatttg ttttattcca tatgggaatt tattttttgc
    12721 acctcttaga ttttcattta tttcatacgg aattagtttc attcttttat tcatcatata
    12781 tcctcctaaa aattttattg cttataaaaa tcatgaattt tattttataa taggtttata
    12841 aatcaaatta aaacctatat ctacttattt atatttatga tatatttttt atttttagga
    12901 gtcatatttt tttgtatata aaataatatt tagataacta tattaaaaaa tacatgtttg
    12961 cttatataca aaacttacta aaatatcttt aatatttttg tatacaaaca gtatattttt
    13021 catacaaaat aaaaaggata cctcattagt gctataacac ctaagatatc caattaatat
    13081 tttcttaatt ttttctaatt ttcgtaaata tagttcacta ttttaaaact gctcctgtat
    13141 ttgctgatgt taccaattta gcatatctac ttaagtatcc atgctttatt ttcggttcta
    13201 aaggtttaaa ttttaatttt ctattttcta tttcaacttc atctactttt aactctaatt
    13261 ttttgtctgg tatgtttata gaaattatat ctccttcttc aactagtccg attaaaccac
    13321 cttccatagc ttctggagaa atatggccta tagatgctcc tctagttgct cctgaaaaac
    13381 gaccatcagt aagaagtgct acatgcttat caagtcccat tcctgcaact gctgatgtag
    13441 gagatagcat ttctttcatt cctggaccac cctttggacc ttcatatctt ataactataa
    13501 catcaccttt gtttattttt ttaccaaaaa tagcatttac agcttcttct tctgaattaa
    13561 aaactcttgc tggtccttca tgaactaaca tttcttctgc aactgctgac ttttttacaa
    13621 cagctccatt tagagcaaga tttcctttca atattgcaag cccaccttgg ttactatatg
    13681 gattctttaa tgtatgtatt acttcttcat tttctatttc acaatttctt atattttccc
    13741 ctatagtctt gcctgttacg gttttgcaat ctaaatttat tccatttatc tttgaaagtt
    13801 cgttcattat agctggtatt cctcctgcca tatgtaaatc ttcaatatga tgttttccac
    13861 ttggacttaa ttttgttaaa caaggagttt tttcacttat ttcatcaaaa aaatctaagt
    13921 taaactctat tcctgattca taagctattg caggtaagtg aagtactgta tttgtagaac
    13981 cagccattgc catatcaaca gctatagcat ttttaaaagc atctattgtt aaaatatctc
    14041 taggttttat gtcgttctta agtaactcca taacatacat acctgcatat tttgctattc
    14101 tttttctttc tccagaatgt gacgcagcag taccattata tggtataccc atgcctaaag
    14161 cttctgttaa acaattcatg ctatttgctg tgaaaagtcc agagcatgaa ccacatccag
    14221 gacaagagtt catggctaac tcttctaatt cttcttcact tactgttcca tctttacatg
    14281 ctccaacacc ctccattgca gagttaaaat catatacttt tccatttttt ttacctggaa
    14341 gcataggccc tccactcaca acaatacttg gtatattaag tcttgcagca gccattaaca
    14401 tacctggcac tatcttgtca caattaggta taagtaccaa agcatcaaaa ccatgtgcca
    14461 tagccatagc ttctattgaa tctgctatta attctcttga agccagggaa taattcattc
    14521 cgacatgccc catagctata ccatcacata cccctatagc aggaaattct aatggcaaac
    14581 caccactcat tcttactcca ttttttgcag cttctgctat ttcatcaaga tgcagatgac
    14641 caggaatcac ctcattttgt gcatttacta tacctattaa tggtctttca atttcttctt
    14701 tagtaagacc cattccatac attaacgctc tctttggagc tccttctatt ccttttttta
    14761 tatcacttct cataatatta cctctttctt acttttttaa attttcataa tattattata
    14821 atatattttt ctattaatta attacgaaaa ttgtaaaaaa atattagcat atactaatga
    14881 aaatttatta cgatttttta tcaatatctt acacaatcgt attatataat attatctttt
    14941 caatatattt tataaactaa tatattttac aaattgttga gtaatatttt atgatataaa
    15001 tcagaagaag cacttctata atagaaagtg ctcaactatg aataagatta ataatatatt
    15061 tgccttagat atatagactt ttataaatat taaagattaa tcaaatctga tgtttgaaac
    15121 tttatcccaa ttaatcttcc ttgaatagtc ttctggatgt gataatctaa ctatatgagt
    15181 tgaattatca tagctgtaat aaattgtagt agtaccatca actggattct ttttgtatcc
    15241 ttgatataca gcactatttg gtactttctt ttcaacaaag ctatatgcag atgaagtatc
    15301 aatagtttct attttattag gaaatataaa catataaaaa gctactacta taaaaataca
    15361 tatgcatgta atactatacc atttaatcca tttcataaaa ttcctcctac tttaccaaaa
    15421 tatactatta ttatttccat atatttataa tatttataca tacgataaaa tttttatata
    15481 cctaactata taattgtaga aactttgatt atatatattt tttaatctta ataatatatt
    15541 tttataaatt aataaatttt taagatttta ttaaaagatt tttaaaactt acagtttaat
    15601 ataaagatag tacttataaa actaaaaaca taaatttgaa aggggacaaa ataaaatgat
    15661 atggacattt tattcattta tttgcgttat attaccatgc ttaatatacc aaatagtcgt
    15721 tataaaacga aaaaatttga aagttgaaaa gaatgtaatg atacatttag tatgggtata
    15781 tattttttta ttatatatct atctagcact tagtaaagct ggaattggta gcatatggga
    15841 tataggaagg tatagtgggc tttttagaat tgatgaaata aatctaatac cttttgactc
    15901 agttggcatc ttaacatata ttttaaatat aattatgttt atgcctttag gatttttact
    15961 acctttaatt tggaaaaatt ttagaaatat tatcaatgtt tcattaactg gattaggttt
    16021 ttcattagca attgaacttt gtcaattgtt taacttaaga gctacagata ttgatgattt
    16081 aatgatgaac acattaggtg ctgttttagg atattttata tgggtaggtg taaataattt
    16141 atttaatctt ataaagaaaa aagatatttt cccatctata tataaaaata ttattcaaga
    16201 tgaaattgct attacaattg atgatgacat aagcaaaaaa attacctctc tttacaaaaa
    16261 tgaagctatt atatatttaa tacttgctgt tttaggagaa tttttattgt ttaattggtc
    16321 cattatacct tattaaataa tttattgata aaacaagtgt acttcgatat ttacaaatat
    16381 gtaaagccct ctttcatact acaaaagaaa gtgggcttta gttattaaac taaaatgcac
    16441 actcactatt tagctggcat ctgaaatatt ttattaacat tgtatctgac tttatttgat
    16501 gttatataat catattttgt tccattttca atgaagtttt gtatattgtc caacacttgt
    16561 atagctcttg tattattttc ataatctcct atttttacag tatcaatttt actttcaaat
    16621 actgcataaa cttgactacc atctatttta atttggttta tctgcattaa attagtttta
    16681 tcttgacttc taattattat catatcaatt cctccattta cttaattcat tttaatttct
    16741 aagaaataat attttataat tgatttatag attatttaaa tttttaaact tagtatttta
    16801 ttttttaata cgattattta tataaattac atctctaata aaaaattctc ttaaagaata
    16861 cttttttgat aatactttgt tttatttgta gattgcattt ttgaccatta taactaaatt
    16921 aaataatcta atgtattaaa gcataaaatt atctatgcta ctgatttatt ttatacattt
    16981 ggtaatttta cttgcaaaaa attttgtaca tattgtttat atctcaatta agggttttca
    17041 aaactttcta tatatttatt aattaattca gtttcttatt tcaacatacc acctctcatc
    17101 aaattaccat ttgtataatt catattatac atatacaaat ggtaattttc aatatatttt
    17161 attattttaa cccttttttt ttatattttt ggtaatttta ttttactttt tatcaaaata
    17221 acttataatt tcggtaagga gtgatgtaca tatggaaagt ttaggagata gaatagcaaa
    17281 tctacgaaaa gaattagata taaatcaaaa agaacttgct actaaagtcg gtataactga
    17341 ggctagcttg tcacgttatg aaaataattt aagagaacct aaaagtgaaa taatagttag
    17401 attagcaaaa gcattagaaa catcaacaga ttatttatta ggcgtaaatg ataatacaaa
    17461 aataagcaaa gaagataaat taatcataga gaacttatca gtaagtgaaa aaacaaaaaa
    17521 gctcttagaa aaaatatatt ctcttgaaaa agaagataga gaagctatag aaaaaatgat
    17581 tgataatgcc tatttaaaaa gattccttaa agaagagaat taattctctt ctttttcttt
    17641 atacaaaatc tcaagtaata attcataaaa agtaatatct tcactcaata aatgttctaa
    17701 tttcattcct atatcttcaa ttttttcttt ttttgtaaca gtagtgtttg acttttccac
    17761 atataccatc ccccaacaaa cttacgttct taatgctagt cagaaattcc caacaaattt
    17821 ttaataaagt ttatgtaatt gtattttgat tttataaaat atattttgat tatccaaaat
    17881 ttccctagat aattatctta attatataat aatactttta ttctacagtt gcaataaaaa
    17941 actcgtaata ataacaa
//
LOCUS       DAEPOG010000070        17915 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEPOG010000070 DAEPOG010000000
VERSION     DAEPOG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.070"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            6..82
                     /locus_tag="KR432_001982"
     tRNA            6..82
                     /locus_tag="KR432_001982"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:His,seq:gtg)
     gene            91..166
                     /locus_tag="KR432_001983"
     tRNA            91..166
                     /locus_tag="KR432_001983"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Lys,seq:ttt)
     gene            174..247
                     /locus_tag="KR432_001984"
     tRNA            174..247
                     /locus_tag="KR432_001984"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            255..331
                     /locus_tag="KR432_001985"
     tRNA            255..331
                     /locus_tag="KR432_001985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:acg)
     gene            344..419
                     /locus_tag="KR432_001986"
     tRNA            344..419
                     /locus_tag="KR432_001986"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Val,seq:tac)
     gene            495..908
                     /locus_tag="KR432_001987"
     CDS             495..908
                     /locus_tag="KR432_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640518.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1217..4648
                     /locus_tag="KR432_001988"
     CDS             1217..4648
                     /locus_tag="KR432_001988"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBH0640519.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4890..6818
                     /locus_tag="KR432_001989"
     CDS             4890..6818
                     /locus_tag="KR432_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0640520.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7271..7735
                     /locus_tag="KR432_001990"
     CDS             7271..7735
                     /locus_tag="KR432_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBH0640521.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            7742..8236
                     /locus_tag="KR432_001991"
     CDS             7742..8236
                     /locus_tag="KR432_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBH0640522.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8252..9277
                     /locus_tag="KR432_001992"
     CDS             8252..9277
                     /locus_tag="KR432_001992"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBH0640523.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9264..11711
                     /locus_tag="KR432_001993"
     CDS             9264..11711
                     /locus_tag="KR432_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBH0640524.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            11891..13264
                     /gene="radA"
                     /locus_tag="KR432_001994"
     CDS             11891..13264
                     /gene="radA"
                     /locus_tag="KR432_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH0640525.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13267..14337
                     /gene="disA"
                     /locus_tag="KR432_001995"
     CDS             13267..14337
                     /gene="disA"
                     /locus_tag="KR432_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBH0640526.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14426..15520
                     /locus_tag="KR432_001996"
     CDS             14426..15520
                     /locus_tag="KR432_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBH0640527.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            15649..17826
                     /locus_tag="KR432_001997"
     CDS             15649..17826
                     /locus_tag="KR432_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640528.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         7460 a         1875 c         3352 g         5228 t
ORIGIN      
        1 ttatggtggg tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt
       61 tcgactctca ttatccaccc caacttatat ggtctattag ctcagtcggt agagcacctg
      121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggcggcgaca
      181 tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc
      241 gcctccaata aaatgcgctc atagctcaac tggatagagt gtctgactac gaatcagaag
      301 gttaggggtt cgagtccctt tgggcgcacc atatgaatag cgagggatta tagctcagct
      361 gggagagcac ctgccttaca agcagggggt cacaggttcg agccctgtta atcccaccag
      421 tgcaaacctc tagtaaaact actagaggtt ttatttttga ttgaaaaagt ttttatattt
      481 aggaggtaag aaatttgtat atagtaatag gaaatgaaat agttgatagt gaagaactta
      541 aaataattat agatgaaaat agtaaattta aagttgaaaa agatttaagt aaaagcacaa
      601 aaagagaaga tgtaatagca tatcagttaa gtatagattt aaattaccta gattctctta
      661 taaatgagca atgtaatttg tccagtttat ctgatgaaga gaaatttgat gaatatatga
      721 ctttgtctga tgaacttgca ctggatttag aagagttaat gccaaaatat acaataataa
      781 atgctagagc atataaatta gacgaggtag atggaattgt aaaaataata ctagctgtag
      841 cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt aaagaggcta tctagacaag
      901 tagattagga tatgaatatg aactatatta aaaaaatgta taaaatctta cgtaaagatg
      961 aaaataatta tgtaaacata gaaaaaaaca gtataaaatc aagtcaattg tagaaatttt
     1021 cacataaatt catctgaaat tctataaaaa atgccttaat tttaatgagg taaatcgagt
     1081 ttacacagtc taaaaattac ttatatttat aaatttatct tattaattaa ggaactttct
     1141 taaacatctt caaaaataaa gtattttagt ctataatctt taatattaca caaacaataa
     1201 aggagaagaa tagagtatgc ttaaaaagtt taataaaata ctagtggcta atagaggcga
     1261 aatagctata agaatattta gagcctgttc agaattgggg ataaaaagtg ttggtatata
     1321 cagtaaggaa gataagtatg gattgtttag aacaaaagca gatgaatcat atttgatagg
     1381 agaaggtaaa gggccaatag atgcatattt agatatggat ggaataatag acctagcaaa
     1441 gagaaaaaaa gttgatgcta tacatccagg atatggtttt ctagcggaga atgcagaatt
     1501 tgctcgtaaa tgtgaagaga atggaattac ttttatagga ccatcatcta aggtaatgaa
     1561 tatgatgggg gataagatta actctaaaaa aatcgcaaaa gaagttaatg ttcaaactat
     1621 accaggagta gaaaaagcaa taagaagtac tgaagaagca aaggaagttg caaacaaaat
     1681 aggatatcct gttatgataa aagcatctaa tggcggtggc ggtagaggta tgagaattgt
     1741 acatcgcgaa gaagacttag aacttgaata tgaaactgct tgtagtgaat caagaaaagc
     1801 ttttggagaa gatattatat ttatagaaaa atatatagct gatccaaaac atatagaagt
     1861 tcaaatatta ggtgataatt atggaaatat agttcactta tatgaaagag attgttcagt
     1921 tcaaagaaga catcaaaaaa taatagaata tgcaccagct ttttcgttag atgataaagt
     1981 aagaaaagag atatgtgaag atgcagttaa gttatcaaaa catgtgggtt attctaatgc
     2041 gggaacatta gaatttttag tagatgcaaa tggtgggcat tattttatag agatgaacac
     2101 aagagtacag gttgaacata ctgtaacaga gatggttaca ggaatagata tagttcaaag
     2161 tcaaatacta atagcccaag gttacagctt agatagtgaa gaaattaata taaaatcaca
     2221 agatgatgtg gaaataagag gttactctat ccaatgtaga ataactacag aagaccctaa
     2281 gaataaattc atgcctgata caggtaaaat tcaagtttat agaactggtt caggatttgg
     2341 tataagacta gatggaggaa atggatttac aggggcaaac ataagtcctc attatgatag
     2401 tttattggtt aagacaatat cttgggatag aacattccaa ggagcaataa ataaaactat
     2461 aagatctata aaagaactta gagttagagg tgttaaaact aatgtaggat tcctagttaa
     2521 tgttttaaat aatcctatat tctcaaatgg taaatgtagt actaagttta tagatgaaaa
     2581 tccagattta tttgaaataa cagaaagtaa agacagagga acaaaattac ttcaatttat
     2641 aggagatgta attgtaaatg ataatgcttg taaagaaaaa ccactatttg atgcattaca
     2701 tgacccaaga atggataagg atggttcaaa atcagaagga agtaaaatac ttttcgataa
     2761 gttaggtaaa tctgcatata tagaaaagat aaaaaatgat aagaaattac tattaacaga
     2821 tacaacaatg agagatgcac atcaatcatt attagcaaca agaataagaa catatgactt
     2881 attaaaagct gctaagccaa cagaaaaata tcaaaaggat ttattctcac ttgaaatgtg
     2941 gggaggagct acttatgatg ttgcatatag atttttaaaa gaatctcctt ggcgtagatt
     3001 acaaaagcta agagaagaaa taccaagcat aatgttccaa atgttgctta gagcatcaaa
     3061 tggagttgga tataaaaact atccagataa tgtaatagaa gaatttacaa aagaatctgc
     3121 tagacaaggt atagatgtat ttagaatatt tgattcatta aactgggttg aaaatatgaa
     3181 accatctatt aatacagctt tagaaactgg aaaaattgtt gaagcaacta tgtgttatac
     3241 aggtgatata ctagataaga ctaaaactaa atacaatcta gaatattata ttaaaatggc
     3301 acaagaatta gaaagcttgg gagcagatat aatagcaata aaagatatgt caggtcttct
     3361 taaaccatat tcagcttaca cactagttaa agagttaaag aaaaatgtaa aagcaccaat
     3421 acatttacac actcatgata caagtggaaa tggtgtagca acttgtttaa tggcgtcaga
     3481 agctggagta gacataattg atgctgcatt agaatctatg gcaggactta caagtcagcc
     3541 atcgcttaat gctatagtag aggcacttaa gaatacagag agagatacag gaatagattt
     3601 atttggatat gatgagttag gaaaatatta taaggattta agaaaagttt ataataaatt
     3661 tgaaagtgat ttaactaatt cttgtgcaga aatatataat tttgaaatac caggaggcca
     3721 atatacaaac ttaaaacctc aagctgatag tttaggatta gttaatagat ttgatgaagt
     3781 taaagaaaaa tataaagaag ctaatgaagt tgtaggagat ataataaaag taactccatc
     3841 ttcaaaagtt gtaggagact tagctatctt tatgactaaa aataaattag ataaagacaa
     3901 tataatagaa gaaggtaaaa atttatcatt cccagattct gttgtggatt actgtaaagg
     3961 tatgattggt caaccagaag gtggaatacc aaaagattta caagaggtag ttctaaaagg
     4021 tgaagaagct ataacagtaa gaccaggttc attattacca gcagaagatt ttgatgaaat
     4081 agctaaatat ctaaatgaaa agtatgatat aaatgctaat ataagaaatg ttataagtta
     4141 tgctttatat ccaaaagtat atgaagatta tatcaaacat ctacaacatt ataatgatat
     4201 atctaaactt gaaagtgatg tattcttcta tggattaaat aaaaatgaag aatgtgaagt
     4261 agaaatagaa gaaggtaagg tattaacaat aagattagtt gaaattggtg aagttaagga
     4321 aaatggattt agaactatag gatttgagtt aaatggaatg gttagagaag tagaaataaa
     4381 agataagaat ttctctggaa aaattaataa tgttgaaaaa gctgatatga atgaccctct
     4441 tcaaattgga gcaagcatac caggaaaagt tataaaaata atggttaaag aagaagatga
     4501 agtaaaagca aatcaaccat taatcgttat agaggctatg aagatggaaa ctataatagt
     4561 tgctaagact gatggagtaa ttaagtcaat caaagtaaaa gaagatgata tggttgaaga
     4621 taaacaatta ttaatgataa tgaagtaatt taattaaaat aaattaaaaa gcactctgaa
     4681 atgtttaata aaaaatgttt tagagtgttt ttatttattt taattaaata tttgttaaaa
     4741 aaaagaggac aattacctcc agatgtagaa ataaagctag taaggaaaat gttatctaaa
     4801 aattctaaat atagtaaata ttttccataa tatttcttag gaaataacag caaagataaa
     4861 atacataaat ttaaaaggtg ggtgcttata tgaaggttta tgatagtaaa atgttgagaa
     4921 atgtagcagt attaggacat agcggatgtg gaaaaacaaa tctaatcgaa actatagctt
     4981 acacagcaaa cactaataag atacctaaat taacagacaa ggtaaatatg acttatagca
     5041 tgggattaat acctatagag tataatgatt ataagtttaa cttattggat accccaggat
     5101 attttgactt tagcggagac gttgtgtcat ctcttagagc aagtgatgca gcaataatag
     5161 taatagatgc aacagcgcct attcaagttg gtacagagaa atcattagaa ctaactgaga
     5221 gtattcctaa gataatgttt ataaataaaa tagataatga aaaggcaaga tacaaagatg
     5281 caatagcaat gttaagagaa aaatacaata ataagatagt tcctatgata agtcctatat
     5341 acaaagataa aaactttgta aagttacata atgtttttga aaacatagat gatttagaag
     5401 gtgaatttaa ggaacaagct atgagtgtta aagaagcatt aatggaactt atagcagaaa
     5461 ctgatgacca aatattagat aaatatttta atggagaaga actaactacg gaagagatac
     5521 aaaaaggtat tattataggt atacaaagag gtgatattat acctgtaata tgtggttcaa
     5581 ctataaataa tataggcaca aaggaaatat tagatacaat atcaagttat ttagaaccta
     5641 tctttacaga ggaaagcaaa ccatttaggg gattagtatt taaaactatg gttgacccat
     5701 ttgttggaaa gatgtcatat ataaagatta ctgagggtgt actttctaag gataaagatg
     5761 tttttaatat aaataaaaat gtaaaagaaa aaatagctaa tatatataca ttgagaaata
     5821 gtgaattagt agaaatagaa aaagctaaag ctggtgatat agtagttata actaaagtta
     5881 attctctaaa aacaggagac actatatctg cagataaaga tgcagaagct ttagaaaaga
     5941 tagattttcc taaaccacaa atatattatg cagtaactcc taagaataaa ggtgatgaag
     6001 aaaaagtagc atctgtatta aataaactag tagaagagga ccctacatta cattggtata
     6061 gaaatacaga gacaaaacaa gcacttttag gaggtcaagg agaacttcat ataaaaacaa
     6121 ttaaaaataa aatgaaagat aagtttggtg ttgatgttga attgaatgac cttaaagttc
     6181 cttatagaga aactataaaa ggaacagccg atgtacaagg aaaacataaa aagcaatcag
     6241 gtggtcatgg tcaatatgga gacgtaaaaa taagatttga aagatgtgaa tctgattttg
     6301 aatttacaga agaaatattt ggtggttctg taccaaaaca atatatacca gcagttgaaa
     6361 aaggtctaaa agactctatg caaaaaggta tattagcagg ttatcctgtt acaaacataa
     6421 aagctacgct ttatgatggt tcataccatg atgtagactc ttctgaaatg gcctttaaga
     6481 tggcagcaag tgcagccttt aaaaaaggta tggaagaagc gcacccaata ctactagaac
     6541 ctataatgaa acttaagata actgttccag aagaatatat gggagatgtc atgggtgata
     6601 taaataagag aagaggaaaa atatttggaa tggaaccaga tgacaaaggg aaacaaataa
     6661 tatttgcaga agctccacaa gcagagacat ttaaatatgc aatagattta agagcaatga
     6721 cacaaggtag aggatacttt gaaatggaat tagagagata tggagaggtt ccatcacagt
     6781 ttgcagaaaa aataattggg ttagctactg caaagtaatg aacaaaataa atagaaaaat
     6841 aattaatata tattattaaa aatattttta gggttttaac taggtatagg tttatgttag
     6901 gtaaacttat acctagtttt tatacatttt tgatgaataa aaaaatatat aatactttct
     6961 taaatgtttt aataaaaaga aagtattttt ttattattat ttttacaaat gtttagtaat
     7021 aaagatgaga aggtatattt aaaatataaa gtatttaaag ttctgaaatc taatcatgaa
     7081 gcaaaaaatt agcatttggt tatcaataaa tataataaat aaaaactcat aattaatgaa
     7141 agaataaatt catatctaaa tttctataaa aaatttaaat ttatacaatt tagggcttta
     7201 aaaatttaaa taataggtgt aaaataatag tcaaagaaag tcaaaaataa aaaggtgtgt
     7261 ggtgatatta atggcaacta tgacagatat aattgaaaaa tttattaaag atttgatgga
     7321 agaagataat agcatacaaa ttcaaagaaa tgaactagca aacttgttta gttgtgcacc
     7381 atcacaaata aattatgtct taacaacaag atttacaata gatagaggat attacattga
     7441 aagtaaaaaa ggtggcggag gatatgttca aatagaaaaa atacgaaaaa gtaaagatgg
     7501 acatattaga gaactactaa atgaaaaaat aggaagtcag atatcctata aaaaagcaaa
     7561 ggaattatta gaaggtttaa aagagtcgga tttgattaac gaaagagaat taaaattaat
     7621 attatatgca atagatgata aatcattgtg tatgccaata tatgaattaa aagagaaggt
     7681 aaggtctaat attttaaaga atataataat aggattattt agtatagagg agtgataatt
     7741 tatgctatgt caaaaatgca ataagaataa agcatctgta tattataata agatagtcaa
     7801 tggagaaaaa acagagatgt atttatgtag tgagtgtgca aaagagaata cagaaatgaa
     7861 ttttaactta gatatgcctt tttcaatgat ggatatattt tctaatttag gttttcaacc
     7921 taaaaaagaa cttgaggaaa agctagtttg tcctaaatgc aatacaacat atagtgagtt
     7981 taagaataat gggagatttg gatgtagtga atgttataat gcatttagta gtcaagtaaa
     8041 tccaatgctt caaaatatac atgggcatat agaacataca ggaaaagcac ctaaaaaatc
     8101 tttttataag ataagtgtag aaaatgagat taaagaacta aaagaagatt tagatagagc
     8161 tataaaaaat gaagagtatg aattagcagc tcaatttaga gataaaatta aatatttaaa
     8221 aggttctatt gactaagatt ggagtgatat tatgaaagag aacatagtca tgaaatcaag
     8281 agttaggcta gctagaaatt taaataacta tccatttcca aataaattag ataaagaatg
     8341 tgccatggaa ataatagaaa aagtaaaaaa tgcgtttatt aattctaact tagaacaaaa
     8401 agaagaattt gatttctata aaattgaaga tttagaccaa tctaaaaaaa tgcttatggt
     8461 tgaagaacat ataataagcc cagatttagc agaaaatgat aaaagtgcag ttatagtaaa
     8521 aaaagataaa actataagca taatgataaa tgaagaagac catataagga tacaaactat
     8581 atgtgatgat ttaaacttag aatatgcata tagtgtagca aatgaaatag atgatttgtt
     8641 agaatcaagt ttagaatatg cctttaacac aaagctaggt tatctaacat cttgtccaac
     8701 taatacagga acaggaatga gagcttctgt aatgatgcat ttaccagcat taagtcaact
     8761 aggctatatg gatgaacttt ataaaatttc atcacaaata ggtatagcaa ttagagggat
     8821 atatggagaa cgaacagaag cattgggaaa tatatatcaa atatctaatc aacttacact
     8881 tggaagaaca gagtctaata ttatagaaaa tgtaagtgga cttacaaaag atgcaatttc
     8941 aaaagaaata aaagctagag aaatattgca aaaaaaatta ggtataaaac tagaagataa
     9001 aatatttaga tcgataggga cacttgaaaa ttcaagagta atgagtagcg cagaggcaat
     9061 gagccatcta tcaaatataa aaatgggaat agaaatgaac tatatagata aactagattt
     9121 aagggctata gaacaactta tgataggtat acaaccagcg catcagtcaa taatgtataa
     9181 gagtgatgat gttgagaata gagatataaa tagagcaaca tatattagag aaactttgga
     9241 aaaattaaga ggagggatga attgtgaact tcaatagatt tacacaaaga gctaaaaagg
     9301 caattgattt agcgtttgaa tctgctaaaa gtctaggaca caatattgtt ggaagtgaac
     9361 atatactttt aggtctttta agagaagaag aaggtatagc tgctaaagtt ctaagtaaag
     9421 ttggatttac agaagcttat ttagaaggca aaatagttga tatggaaggt aagggagaag
     9481 aaatctcaga agatatagta ttaagtccaa ggagtaaaca aatacttgaa ttatcaggaa
     9541 tgtttgcaaa taaattaaaa acaaattata ttgggactga gcatatatta ttagcaatta
     9601 ttcaagaagg tgaaggaata gctaataaga ttttaaatta tgctggagta aatgatagga
     9661 ctttagccca attaacaatt gatatgatgg gtattagtga taaaaatcaa tacaaagcag
     9721 agaatagtta cacgggcaac cagaatcaag cagaatctaa agtacttgat aagtatggaa
     9781 gaaatcttac actatatgca aaacagaata aaatagaccc tgtaataggt agagaaaaag
     9841 aaatacaaag agtaatacaa atattaagta gaagaactaa aaacaatcca gtgttgatag
     9901 gagacccagg tgtagggaaa acagccatag ctgaaggtct ggctacaaat atagccttag
     9961 gaaatgttcc agaaacacta aaaaacaaaa ctttatactc tttagagatg ggttcattat
    10021 tggctggggc taagtataga ggagaatttg aagaaagaat taaagaagtt gtagatgaag
    10081 ttgttaaaaa tggaaacata atcttattta tagatgagat gcacacaata ataggggctg
    10141 ggtctactgg agaaggctct atagatgctt caaatatatt aaagccagcc ttggcaagag
    10201 gagaaataca agttataggt gcaactacaa ttgatgaata tagaaagcat gttgaaaaag
    10261 attctgctct agaaagaaga ttccaaccag ttatggtaga tgaaccaact aaagaggact
    10321 caataaaaat attagaagga ttaagagata aatatgaagc tcatcataaa gttaaaataa
    10381 ctgatgatgc tataaaggca gctgtagaat tatcaactag atatatatca gacagatatc
    10441 tgccagataa agctatagac ttgattgatg aagctgcatc aaaagtaaga ttaaaagaaa
    10501 atacacctcc ttcagaaata aaaaaattag aactagaaat agaaaatata gataaagaaa
    10561 aagaagaagc agtaagatgt caagactttg agaaagcagc caaaataaga gatgaacaag
    10621 gtctacttaa aaaacaatta gaagatgtta gagaaagatg gaacaagtca tctaaacatt
    10681 cagatttagt tgatggagaa gttattgctg aagtagtggg cttatggaca ggaataccag
    10741 ttaataaaat tcttgaagaa gaggctgata gacttttgag acttgaggaa atactgcaca
    10801 atagagttat aggtcaagaa caagcagtaa aatctatttc aaaggcaatc agaaggtcaa
    10861 gagcaggtct taaagaccct aatagaccaa ttggttcatt tttattttta ggtccaacag
    10921 gagtaggtaa aacagaatta tctaaggcat tagcagaagt gcaatttgga gatgaaaatc
    10981 aaataattag aatagatatg tctgaatata tggaaaaaca tgctgtgtca agaatgatag
    11041 gttcacctcc aggttatgta ggtcatgatg aaggtggtca attaactgaa aaagtaagaa
    11101 gaaatccata ttcagttatc ttatttgatg agatagaaaa agctcatcca gatgtattta
    11161 atattctgtt acaaattcta gatgatggta gactaactga ttcaaaagga agaactgtag
    11221 attttaagaa tactattgta ataatgacat caaatgttgg agcatctaca attggtagac
    11281 aaaaaacttt aggatttagt atagctaaag gagacgaaga agaaaaatct caatatgaaa
    11341 aaatgaaaga aaatataatg ggtgaattaa aacaaagatt cagaccagag tttttaaaca
    11401 gaatagatga tataattgtt ttccactcac taaatgaaaa tcatatatca aaaatagtac
    11461 ttttaatggc agcgaaacta caagagagat taaaagaaat ggatataaaa ttagaaatga
    11521 gtgatgaggc tgttaaatta atttctaaat ctggatttga cttagaatat ggagcaagac
    11581 ctcttaaaag agctttacaa aaagagttgg aagatgagct atctgaagca atcttaaaag
    11641 gtaatgtgaa aaagggaagt aatgtagtag caaaagtcaa agatgaaaaa atagtttttg
    11701 aaactaaata atttaataga gttgcatttt aaaatattgc aaatataagt tttataatct
    11761 aaacaaaatt ttaagggcaa tgaatctata ataattcatt gtccttaatt attttattta
    11821 atctttacat attgtctttc agatatgcta aaattatagt atagaataaa aaattaagga
    11881 tgataaaagt atggcaaaaa taaaaacaaa atatgtatgt caatcttgtg gatatgaaac
    11941 agctaaatgg cttggtaaat gtccagaatg tacaaaatgg aatacatttg tagaagaaat
    12001 agaccaaaaa agcaccaaaa aagaggtctt tataatagat aaatcttcat ctaaaccagt
    12061 cagtataaac tcaatagaaa gtaaagaaga agagaggttt acaacagata taaatgaatt
    12121 agatagagtg cttggaggag gtatagttaa aggctcttta gtgcttgttg gtggtgaccc
    12181 tggtataggt aaatcaacac ttttaatcca agtatctagt aatgttgcca atttaggaaa
    12241 aactgtttta tatataactg gagaagaatc agaatctcaa ataaaaatga gagctaaaag
    12301 attaggtatt aactcagaaa atttatatat atttgctgaa aataatttaa gtataattga
    12361 atcttattta gaaagtgtaa atccagaatt aataattcta gactcaattc aaactgtctt
    12421 cagtccagaa atatcctcgg caccaggaac tgtcagccaa attaaagaag gtacttcaaa
    12481 attcatgaaa atttctaaaa aaatgggaat ttcaacgttc atagttgggc atgttacaaa
    12541 agaaggttca ctagcaggac caaaattatt agagcatatg gttgatactg ttctatattt
    12601 tgaaggagaa agatataata cttatagatt agtaagagct gttaaaaatc gatttggttc
    12661 aactaatgaa cttggagtat ttgaaatgag ggatttagga ttagtagaac ttgataatcc
    12721 atctaaaatt ttaatctctg aaaaaccaaa agatgtagca ggttcagtaa taatatctac
    12781 tgtagaaggt acgagaccaa tgttacttga attgcaagca ttagtttctc ctacaagttt
    12841 tggtatacca aaaagaacat ctacaggagt cgattacaat agagtaggta tgttattagc
    12901 agtattagaa aaacgtgttg gtttgcaaat acaaaatcaa gatgtctata taaacatagt
    12961 tggaggaata aagataaatg aaccctcaat tgatttaggg atagccattt ctgtagcatc
    13021 tagttttaga aatataccga tagatgaaaa tatagctgtt acaggagaag tgggacttac
    13081 aggagaagtt agagcagtaa gctttataga aaagagaata gctgagtgta aaaaacttgg
    13141 atttaaaaag atagtagttc ctagaagtaa ttatgatgtt gtaaaagaga ccaaaggcat
    13201 agaaatatgg ccagtagata acttaagaca agctataaat atagtacttg ggaggaatca
    13261 ataaatatgg agaattttct agataataaa aatatgctat atgcattaaa aatgatatct
    13321 cctggaactc cacttagatt aggtctaaac aatgtactaa gagctaagac tggtggatta
    13381 attgtaattg caacaaacga agatgtaatg aaaatagtag atggaggatt tgctataaat
    13441 gcagaatatt caccatcata tctatatgaa ttagctaaaa tggatggagc tatagtttta
    13501 agtggtgatg taaagaaaat attatttgct aatgcacaac ttatacctga ctattttata
    13561 gaaacatcag agacaggaac aagacataga acagcagaaa gagtagcaaa acaaactggt
    13621 gctatagtca taggaatttc acaaagaaga aatgttataa cagtttatag aggaaatgag
    13681 aagtatgtag tcgaagatat atctaagata tttactaagg caaatcaggc tatacaaact
    13741 ctggaaaaat ataagacagt attggaccaa gctgtaacaa atttaaatgc cttagagttt
    13801 aatgatttgg taactattta tgatgttgca ttagtcatgc aaaagatgga aatggtaatg
    13861 agagttacaa gtataattga aaaatatgtg atagaattgg gtgatgaagg aactttagta
    13921 agtatgcaat tagaagaatt aatgggtaca accagaatag accagaaatt aatattcaaa
    13981 gattataata aagaaaacac agaaataaaa gaacttatga aaaaggtcaa aaatttaaat
    14041 tcagaagaac taatagaatt ggtcaatatg gcaaaactat tagggtatag tggtttttca
    14101 gaaagtatgg atatgcctat aaaaacaaga ggttatagga ttcttagcaa aatacataga
    14161 ctaccaacag caataataga aaacttagta aattattttg aaaactttca acaaatttta
    14221 gatgcatcta ttgaagaatt agatgaggtt gaaggaatag gtgaaataag agcaacatat
    14281 ataaaaaatg gactcataaa aatgaaacaa ttagtcttat tagatagaca catatgatga
    14341 attttaaatg aattttacta cttttttctt gaatataata tacactttgg tctataatag
    14401 ataagtataa ctagggggtg aagcattgat aaggaaagta acaagaatat tgtttatttt
    14461 acttggattt acaataggta taacaaccta tttaacatta atgaaagatt ttgaaatatt
    14521 aacatttgga aaagaaacat atggttatat cgcagctgta gtagcaggta taataatagc
    14581 cattttagga tatcttatag aaccatgggt tgtaaataaa gtaaaagaaa ttgctaaaat
    14641 agtggataaa gaattgtcaa aatatcctca gactgatata ttgttagggt ctatgggtct
    14701 tatagtagga tttgttattg cgtatttatt aagtggatta gtaaatagaa taccaatagt
    14761 aggtggaata ctatccttac tattatattt attcctagga tatcttggaa tgaaagttgc
    14821 gcttaaaagt aaaaatgatt tatttaatat tggaaaacta ggaagattag caaatcctat
    14881 taaggataaa gataaggata aagaaaataa aaaagaagta aaagccattc ctccaaaagt
    14941 attagataca agtgtaataa ttgatggaag aatagctgat atttgtaaga ctgggtttat
    15001 agaaggaaaa cttattattc ctgcttttgt tttggaggaa ctaagacata ttgcagattc
    15061 ttcagatgat ttaaaaagag taagaggtag aagaggtctg gatatattaa atataattca
    15121 gaaagaatta aatatagaag ttgaaataag tgagagagac tttgatgata tagcagaagt
    15181 tgatagtaaa ttattaaagc ttgctcaggt tttaaatgga aaagttgtta caaatgatta
    15241 taacttaaat aaagttgctc aattccaagg tgttgaagta ttaaatataa atgagttagc
    15301 taatgctata aaaccagttg ctataccagg tgaagatatg gttgttcaag tggttaaaga
    15361 aggaaaagag gctcaacaag gtgttgcata tctagatgat ggaactatga tagttgttga
    15421 tggaggaaga aaatatatga atgagactat aaaagtactc gttacttctg ttcttcaaac
    15481 tcctgcaggt agaatgatat ttgcaaaacc taaaaattaa tatgttcaaa gaaacctata
    15541 atttgggctg tttattttaa atagatggtc caaatttatt tatttataac gataatgttt
    15601 aatgtattct taaagatggg gatttaaatc agaaagcagg gagaatttat gagaaaaaag
    15661 attatatctt tcttagcaac atttatcata atattaacat cagcaagtca gtatagtttt
    15721 gcagatgata tgtcaacacg tggaaaagta atatttatag atatgaatag aactagtatg
    15781 agtaatatgt taagaataaa atcattaaga gaagaactag acaatagagg gtatattggc
    15841 ttgatgaata taagaggcga taaaggaagt gatgatagaa ggtcatatgc tagtatgggt
    15901 gctggtggta gagctaatgt ggctaatgaa gaagatatta attttgaaag ctcgagtaag
    15961 gatagaaata tagtttttga atcagctaca ggaaaaagtg caaaaggtat aaataattta
    16021 actataaata agtctataaa tgaaaattta aactttggag agtatggttc tgtattaggg
    16081 tcattaggac aaagtttatc tgagaatgga ttaaaagcat ctgtattggg taattcagat
    16141 ataattgaaa atggtcaact tataaaaaat agaaacttat gtctaactgc aatggatgaa
    16201 tatggaagaa tacctaatgg aaatgtagat actataaata aaaaagactt atctatgcct
    16261 tatggaatat caactgatta tgataaattg attgtagaga ccaaagaagc ttacaaaaat
    16321 aatgatgtgg tttttgtgga gcttggagac acatatagat tagatttata taaaccaaat
    16381 ttaaatgaaa aaacatatga gagtgtgaaa gataacatag aaaaaaatat agatgtatat
    16441 ttaaaaaata tattttctat ggttgaagaa aatgacactg tctacatagc aagtgcattt
    16501 ccaagtgatt tagattataa aaataaaaga agactttctc caataataaa attaaatgga
    16561 gaagggaaag gtatattatc atcttcaact actagaagag aaggtattgt aacaaattta
    16621 gatgttgggg ttgaaatatt agacaacttt aatataaaaa atcaaaatat ggttggaaga
    16681 aaatatgaac ttattaacag agatgataat aaagaatttt tgatggatga atatcaaaag
    16741 atagtatcta tttcaagcat aaggtctaca atactgaatg gttttgtaag tatagttttc
    16801 ttgtcatgga tagttgctat gattgcaatt ttatttagaa agcatatatc aaaaaattat
    16861 aaagaaacaa cattttttat attaaaggaa cttttaaaaa taggaatagt aatgccactg
    16921 tcatttatga taacaccaat aatgaatttc aaaacaccat tagcaatatc tttaggaata
    16981 attataataa cacttactat atatcttata agtaaagttt taataaagaa tgatttaaaa
    17041 aacatgctgt tttttactgg attaaccata gtaattatgg ttattgatgc tgggtttggc
    17101 tcatatttaa tgaaaagcaa tgttatgagt tatgactgta taattggagc tagatattat
    17161 ggagtgggaa atgagtatca aggtgtggca atcggtagtg caatatttac atttgcaatt
    17221 ttacttacat ataaaaatat accaaagtgg tctataatag ttttttcttt ggttatctta
    17281 attacaagtg catctccaat tatgggagca aatgttggaa gtgctatttc tgaatgtgtt
    17341 gcatttttac tatttatact attgatatat aatgtgaaga tagacttcaa aaaaattgta
    17401 ttattaggta tagcagtatt atttgtatta ggagtttttg tagcaataga tatgattttg
    17461 ggaagtaatt cacatttagg tatgtttgta aaagaaattt actttaatgg accaggagaa
    17521 ataatacaaa catttagtag aaagatagag atgaatttaa agctagctca aaccagtgca
    17581 tgggtaaata tactgttaac tggaataggt gtaattttag ttcttatgat taatcagatt
    17641 agatacttta agcaactgat ggatgagtat ccaattgtat ttaaaggatt tatagcatca
    17701 atagcaggat gtttagtaac tcttttagtg aatgactctg ggattgtgtc atcagctaca
    17761 gcctttattt atgtaattgt acctatgata acattaagta taaatctcac agcattaaaa
    17821 gaataagtac tgtatgaata atatgtaaat atgaacacat taatttaact tctttattta
    17881 caaaataaag aagaaagttt ataaaaaata aatta
//
LOCUS       DAEPOG010000071        17865 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEPOG010000071 DAEPOG010000000
VERSION     DAEPOG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.071"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(124..270)
                     /locus_tag="KR432_001998"
     CDS             complement(124..270)
                     /locus_tag="KR432_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBH0640529.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(948..1472)
                     /locus_tag="KR432_001999"
     CDS             complement(948..1472)
                     /locus_tag="KR432_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0640530.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(1537..2289)
                     /locus_tag="KR432_002000"
     CDS             complement(1537..2289)
                     /locus_tag="KR432_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0640531.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(2320..3618)
                     /locus_tag="KR432_002001"
     CDS             complement(2320..3618)
                     /locus_tag="KR432_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBH0640532.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(3635..5899)
                     /locus_tag="KR432_002002"
     CDS             complement(3635..5899)
                     /locus_tag="KR432_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBH0640533.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(6154..6972)
                     /locus_tag="KR432_002003"
     CDS             complement(6154..6972)
                     /locus_tag="KR432_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0640534.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6965..7663)
                     /gene="cbiQ"
                     /locus_tag="KR432_002004"
     CDS             complement(6965..7663)
                     /gene="cbiQ"
                     /locus_tag="KR432_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBH0640535.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(7666..7950)
                     /locus_tag="KR432_002005"
     CDS             complement(7666..7950)
                     /locus_tag="KR432_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0640536.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7940..8692)
                     /locus_tag="KR432_002006"
     CDS             complement(7940..8692)
                     /locus_tag="KR432_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBH0640537.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8881..9073)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9205..10977)
                     /locus_tag="KR432_002007"
     CDS             complement(9205..10977)
                     /locus_tag="KR432_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBH0640538.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10991..11920)
                     /locus_tag="KR432_002008"
     CDS             complement(10991..11920)
                     /locus_tag="KR432_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0640539.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            12149..12337
                     /locus_tag="KR432_002009"
     CDS             12149..12337
                     /locus_tag="KR432_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640540.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            12330..13031
                     /locus_tag="KR432_002010"
     CDS             12330..13031
                     /locus_tag="KR432_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640541.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            13033..13956
                     /locus_tag="KR432_002011"
     CDS             13033..13956
                     /locus_tag="KR432_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640542.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            14113..15036
                     /locus_tag="KR432_002012"
     CDS             14113..15036
                     /locus_tag="KR432_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640543.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            15029..15766
                     /locus_tag="KR432_002013"
     CDS             15029..15766
                     /locus_tag="KR432_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640544.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            15777..16550
                     /locus_tag="KR432_002014"
     CDS             15777..16550
                     /locus_tag="KR432_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640545.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            16566..16718
                     /locus_tag="KR432_002015"
     CDS             16566..16718
                     /locus_tag="KR432_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640546.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(17091..17699)
                     /locus_tag="KR432_002016"
     CDS             complement(17091..17699)
                     /locus_tag="KR432_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH0640547.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5793 a         3064 c         2203 g         6805 t
ORIGIN      
        1 acttattctt ttaatttcaa tatctacttt ttctttaaaa atatgattct tatttgaatc
       61 aatatgaata aataactctg cttttaatat acttttgtct tttaatatat ctggtattct
      121 cacttaaaaa aaagtaatca acttttcttt tacaaaacta tttgaacatt tacttcctat
      181 taagcaataa tctaaattac taaagttttt atctggatat tttttagata taaaagtatc
      241 atcatttaaa taaagtataa tttttctcat ttataatccc tccattttgg gtctatatca
      301 tactatgttt aaaatacatg cttagttaca atacaattaa aatttatagt attaaatttt
      361 tatataataa tgctgatttc tacaaaaaaa gaacatgtca ttagaaactt ctaatagcat
      421 gttctttttc attattatat attacacttt aaattattcc ttgactggct tagctggtgt
      481 aaactaaacc agtctttcta aaatattaag cacttttttc ttattatgtt ttttaggatt
      541 tattgtctct attattgtaa accaaagtta taaaattgta aatagaatat attaccataa
      601 caatattttt ttataagtaa aaattataaa aaaccttatt tttattaaat agaataaata
      661 aaataagttt aaaaaatact gaaataagtg ttttccacat attttatata tttatttaat
      721 acattattat ttaaaaataa atatataaac aatcataata aaattttttt aataaaactt
      781 tattttttag ttaaaatcat acaatatata gctttccaca atacaaataa attaaaaagt
      841 gaaatgtatt tgtctaaaaa cataataaca tttcacttta tcctcatttt taagtaatat
      901 atatttttaa tttttattta taactcaatc tagtttttat ataagtttta gtttttttct
      961 acatctgagt aatctattcc aaaagtttct acagtaacag acttcataat ttgtggagtt
     1021 tgtggcttat cagctgaatc tgttctaact gatgctattt catcaactgt atccatacct
     1081 tctatcactc taccaaaacc tgcatactga ccatctaagt gtggtgagtt tttatgcatt
     1141 atgaaaaatt gagaaccagc tgaattaggt gccatagttc tagccattga tatgactcct
     1201 ctttcatgtt ttaaattgtt agtaaatcca ttaccactaa attcaccttt tattgagtga
     1261 cctgggccac ccattcctgt accatttggg catcctcctt gtatcataaa tcctggtatt
     1321 actctatgaa atattattcc attgtaatat ccttcattta ctaatgatac aaagttttta
     1381 actgtatttg gagctatatt tggatataac tcaattttga tttcttttcc attttccatt
     1441 tctatagtta ctataggatt tttattttcc ataatttatt accttccttt tcattagtta
     1501 aattacataa ttacatttat tatattatac tctaaattaa agtctatcaa gtatattttt
     1561 tcctatttcg tatccttctt gataaatagt tttacaaaac tcatcatcaa agttaagcgt
     1621 atctttagga gcaaattgag tattaggtct aactactatt attttatctt catcatatac
     1681 ttttttaata tcctctggca actcataatt atattttgtg gatattaaaa taattttatc
     1741 cacattatcc ccaattaatg gttcaattaa tgcactccta attaaacctc catctaattt
     1801 gaaaccatca taaattcctt cttgcacatc tttcacaaat tgttctttaa atccaagagt
     1861 tgcgtttgga tttgatggta acaacccact gtatgaaatg ctgataatgg cttcattacg
     1921 aggcattttt gatatgtcaa caatcttttc tgatactact ttattatcaa cttcaacata
     1981 attaacataa aagttcattt cttctatatt actatcatct agttccctta ggttgtctat
     2041 agcaggagaa ttatccacag tattatttac tatttttaca tttccattct ggatatcttt
     2101 tatatttgtc cacattttct ccaagttatc aacattccct gtgtacaaat aatatccatt
     2161 tattgctcct attgatgtcc ctgaaatcga ataaaattta tttattcctc catcatacaa
     2221 ggctttaact actcctgctt gataagcccc ttttgctcct ccaccttcta aacataatcc
     2281 taatttcata gtctcatctc caaattgttt atcttatatt tatatttcaa aaaacttact
     2341 aaatgtttta tatatactat agtggtcctc aacatttcct aattctctta tagagtgcat
     2401 tgctaaaatt ggacttccca catccacaga tggtatatct atatgagtag aagatattgg
     2461 acctatagta cttcctcctc tttcatctga cctattaaca aattcttgat actcaacatt
     2521 aacttctcta catatatttt tataaatagc tatagaatat ccatcactag tatatgcctg
     2581 actagcattt atctttataa ctggaccttt tcccattatt ggtttattag ttgggtcatg
     2641 cttttctgga atgtttggat gaatagcatg agctaaatct gatgatatca taaaagatga
     2701 ataaatagac tcaaagaatc cctctctatc cttacccatt gatatgcaaa ttctctccaa
     2761 tatatttaac aacatgtttg aatctgcacc ttgtttagta gagctaccaa cttcttcatt
     2821 gtcaaataca gccattacat ttacaccttg ttgaccttta gtattaacaa gagcattcaa
     2881 actagcatgt accattgcaa gattatcttg tctcccaata gatataaatt catcattagc
     2941 ccctattaaa cttccttttt caaattcata taaaaataaa tcaaaatcta taatatcttc
     3001 catatttaca tctaagttag atgctatttg ctttataaga aaattatctt tttctaatgt
     3061 atcatttaac atcccaacta aaggtaacat atctttttgc ttattatatg aatgaccatc
     3121 atttatactt ctatttaaat gtattgctag atttggtatt atacaaattg gctttttaaa
     3181 gtctataagc ctttcatttg gttttaatat actatcacct tttaaaacta ctctaccagc
     3241 tatcccaaga ggtctatcca accatgtact taatattgct cctccatagc attctgtatt
     3301 taacttcaaa taagattttt cagcttctat ttctgcattt ggtttaatac taaatgtagg
     3361 agaatctgaa tgagagccta ttattctaaa tccatctctt tctatatctt ctgaatttac
     3421 tacaaaagct actatagctg atagattctt agtaacataa tactttccac caactcttag
     3481 attccatctt tgattcaaaa ctaattcttc aaatccattt ttatctaaga tttccttagt
     3541 actctcgact gcatgaaaag acgacggact atcatatatg aagtctatta agtttttagc
     3601 aaagtttata ctatccattc ctcatttacc tccattataa aatatttagt gttgtatatt
     3661 ctttatttat ttttttaatt ccctgttggt caaaagctat agtaacatct gtaccaatta
     3721 tagaaaccac tgtcccaacc ccaaatttag gatgatgtac cttagaacca agttttatat
     3781 catctggatt agtttcttta ttagaatctt ttattgtagc attaacttta tctgcaactt
     3841 tagatttgct tatagtactc atatactttt tagtatattt atctaacata ttataacttg
     3901 ctttagaata gcttagttct ttttttactt tgtacaatct ttctatacat tcttctggca
     3961 gttcttccat aaatcttgaa gctattgcaa catttgtttt accatataat gttctcttct
     4021 ctgtcaatga catatatagc tcttctttag ctctagttat accaacataa caaagtcttc
     4081 tctcttcttc tatttgtgaa tcactcatag attttatagc tcttgatata ggaaataacc
     4141 cttcttccat acctgttaaa aacacaacag ggaactctaa acctttggat gtatgaattg
     4201 tcataagcga aaccctatca ttgtcttctt catcatttga ttcagatgta agtgctatac
     4261 ttgttaggaa tgtctctaag tctttatttt cataagtatc atcattacta ctttcaaact
     4321 ccaaagctat agaaataaat tctctcaaat tatcaattct gtcttgagct tcttcacctt
     4381 tgcctgttaa atctttttca tttttatttc ttatctctac aagctcgtcc atatatcctg
     4441 tagtatccaa gactttttct ataagtttgc taacaggata aacttctttt atagttctaa
     4501 gagtccctat aatatcaaca aatccactta tacttgccct tgccttagta gatatatctg
     4561 aatttgtttc tatatcaatt aatactgagt atatactttc ctgttttaag ttagctctat
     4621 cttcaatttt ttctatagtt ctaagcccta tacctcttct aggaacattt ataatccttt
     4681 ttatagatat atcatcttgt ggattttgaa taactcttaa atacgccaac aaatccttta
     4741 tttcttttct ctcgtagaat tttgtaccac catatatatt gtatggaatt tgacttctat
     4801 ttaaagcgtc ctctacaggt cttgcctgtg catttgctct ataaagtact gcaaaatcct
     4861 tataaggtct attttctttt ctagctattt gtgcaattga atcagcaatg aaatcagcct
     4921 cttctatttc gctaccagtt aattgtatct ttataagttc tccttctttt ttttcactcc
     4981 aaagcttttt tctttttctt tctatattat tagatataac tttattagct gcatctaata
     5041 tcacttgtgt agacctataa ttttgttcta actttacaac atgtacattg tcataatctt
     5101 tctcaaattc aagtatgttt cttatatctg cgcctctcca tccataaata ctttggtcat
     5161 catctccaac aacacaaata ttttgatgtt ctcttgctaa taattttatc aattcatatt
     5221 gagctttact tgtatcttgg tactcatcta ccattatgta tctaaatcta cttctgtaat
     5281 atgctaatac ttcatcatta gccttgaaaa gttctacagt tttaagtatt aaatcatcaa
     5341 agtcaagtgc actatttctt tttaaacggt cttgatataa cgcataaata tctgcaatct
     5401 taaccattct attatctgcc atgttgatat ctttaaactg ctttggagta taaagtttgt
     5461 cttttgctcc tgaaattgca cttataactg cttttggttc aaacacttta tcacttaaat
     5521 ttaattcttt tagacagtct ttaaccaatg taacttggtc tgctgaatca taaattacaa
     5581 aacttctatt gtaaccaatc ttatttatat cttttcttag tatacgaaca cagcaagaat
     5641 ggaatgtact tatccacata tccttagttt caggacctat attttgttca actctctctc
     5701 tcatttcatt tgcagctttg tttgtaaatg ttatagctaa tatatttggt gcttgtacac
     5761 ctttatcttc tataagatat gctattcttg ttgttaatac cttagtttta cctgaaccag
     5821 caccagccag tataagtact ggaccttcag tcttttctac tgcttctctt tgagcaggat
     5881 ttaatgtatc taaattcata tttctcctcc tgttaacagc cataaatata ttttaatcta
     5941 aaatatgaat tacttcaaat tataattatg tattatatta atagattatt tccaatatat
     6001 tacaattaaa taacattttt ccatataatt tatttaattg tttaataaaa aaaataacct
     6061 tatgatttgg ggaatcataa ggcaccttta aggggtctag gttaagggtt attatatata
     6121 aatctatttt tttaaaatag gtttagggct taattattta tttgtattaa gttctctttc
     6181 cttccaatac ttatacaaat cttcttcttt cttaaataag ggtaaattca tatttctatg
     6241 tacttttact aaccatgggg attctaggcc agcttgtata atgttatttt catttacaaa
     6301 tacattttct gcctttcctt catcaattaa aatcccctta tccaatacat atatataatc
     6361 gcatatttca tacattaaat tcatatcatg actggatatt actattttta tattatcttt
     6421 attcaatccc tttattatat ctacaataga cctagtactt actgggtcta aaccagctgt
     6481 aggctcatca agcaatataa tttcattttc cattgctata actgatgcta tcgctaccct
     6541 ttttttctga ccataactta aaaaatgtac tggtctatct ataaaatcta ttccattaac
     6601 agctactaga gctttgttta ttctctcttt aattattttt tcatctatac caatatttct
     6661 cattgcaaag gctatatcat catatacttt tgaataaaaa atttgttttt ctgggtcttg
     6721 gaacacaata cctacatttt ttctaaggtt gtataaactt ctcttatcat attttaactt
     6781 ttcttcttta aaaagaattt ctccagatgt aggctttaat atacccatta aattcataaa
     6841 taatgttgat tttcctgaac catttgaacc tattatacct ataacatttc cctttgaaaa
     6901 atccatatta atatttaata gtgcattggt attcttttca tattgataag ttaaattatt
     6961 tattttaaac atcattatcc cctactatgt gaaatttacc atcatacaat tttatgtcta
     7021 atgatataca catatcatca taccttgtca taagtctttt gtacagcata ctgccaagaa
     7081 ttcctaatga tttatatgaa gtttttaaat ttatgtaccc aaatcttagt tcttgtgact
     7141 tatatatttc tgaaaattct tctaaaaata tgaatataaa cctatatatt aacattgata
     7201 gctcaacaaa agtatcaggc aaatgcattt ttttaaagaa gaatattaat tgattaaaag
     7261 gtgttgtaag tatacaaaag tatacacaag tcaaacaaga catagctcta aataacacat
     7321 gaatagacat gtcaatagat gcttttgata ttcctacatt gaaactaaaa atttcaaaac
     7381 tatataataa gtctgcctta ttaaaagata tatttacaag agttattcct atactaagaa
     7441 ataaaaagta cattggtatt ctgagtaatt ttaaataatt ctttaaatct attccagcta
     7501 cacaaacaac tagaatactc attaaaatca taattccact taaaacaaat atatttttta
     7561 ttaccataga agcaatcaga aagattactc ctatggctac ttttttattt ggatttactt
     7621 tacttaatct atttgtatat gcatatttat caattataag cataattaac ttctctttcc
     7681 tttttgaaca cctaatatgt accctattac accagcacct aacgctgctt gtgcagagaa
     7741 taataaactt tctatttctc cacttggtgg ctcatatggg ctactaaacc atggttcata
     7801 atctggattt atcttagtaa tttcactctc agcttgacca tctgctcctc catattcagc
     7861 tcccgaatta acaagtaatg ggaatatgat aagagctact actaataaaa gtagtaatat
     7921 attagttttc gtctttgttt tagcactcat tagctttaac tcctttatca ttattttcta
     7981 gtatgctttt ttctccattt tctgtaatta aattatatac aattacagta agaataccct
     8041 ctgctattgc tattggtatt tgagtcataa agaaaattcc taaaaactta attgctgaag
     8101 ccatcactcc acctgatggg tctgggaaag ccagtgctaa ctgtatcgaa gttactgtat
     8161 atgtagccaa atctcctatt gctgctgcaa gaaatactgg cattgcatta tttccgtctt
     8221 ttttctttaa tgctttaaat attaagaatg atataattgg tcctataata gccattgaaa
     8281 aagcattagc tcctaaagta gttattcctc catgtgccaa tagtaatgct tggaatatta
     8341 gaactatagt tccaagaaca aacattacac ttggtccaaa cattattgct ccaagaccta
     8401 ctccagtagg atgtgaacaa cttcctgtta ctgaaggtat ctttagtgct gacaatacaa
     8461 atacaaatgc tccacacagc gctaataata ctttcttttt agggtcttgc tttactattt
     8521 ttcctattga ttttagtcct actaaaaaaa atggtataaa tataactccc catgctatag
     8581 accattttac tggtaagtat ccttccatta tgtgcattgc attcgatgta actggagtta
     8641 aaacgattaa cataagtgca gctataaccc ctaatttgat attttgtttc attttactat
     8701 cccctctctc cctttctaat aattaaaata taaataatat atgtctgatt tattttaaaa
     8761 ataaaaataa aaaagcctaa ctcgatggct aggcttctaa ttttcgataa tccaaataca
     8821 aacacataaa atgaacatat ctgtataatt attataagaa actttctcca tccccgaaga
     8881 aattacttta atattttagg caggtctcct gacttagctt cttcctactc ctcttccttc
     8941 ccatgagata taaaaaatat catcatagtg gctaacaaaa gtttcgtctg catcacagta
     9001 gcgggggctg taaaggaatt taaccttttt ccctattaat cttgcttaaa gaacctataa
     9061 tattatatat ttaattttta aattattctt aaatcatttt ctatataaat cattgtatac
     9121 cttttctgtg aaaatttcta cattttttat aaattttatt catttttata tttttatgta
     9181 tataaattct tttttgtaag tttattaatt tctaattatc ttctttaaat acattgaaac
     9241 tacatcagaa aatctagtta tatctcttga atatataaaa tcatttccat atattaatct
     9301 ttcagcttca agttctgatt cttttcctgt aaatactcct agtactaata tattttgctt
     9361 tctagctttt ctcacttcat tggctgtatc tctcaaacca actatacccc tataagcctt
     9421 ttctcctctt aatgttctct cactactttt tcctatcttt acatcatttg gtcttccatc
     9481 gctgagaact atcaggattt tattttcttc atctctattt attagaccac tacatataga
     9541 ttttattgcc aaaccatctc tattattacc agcacaaaaa tattcaaata tattttcact
     9601 attttttata ctatcatcat aatctttaaa tctttttaat attgtatagt ctaaaaaact
     9661 agaaaatccc atcactctat ttggtatgcc aacttctgtt agagcatttg caattatgta
     9721 agcttgaatt gccacatttc cctgatttct actttgagag ccacttgcat ccaataatat
     9781 atcaataacg tatttccctt tttcattttc tatatcttta taaaatattt tattattatt
     9841 acttctacca actctccaag ctctatttgc taatatagta ccacaatcag aatatactct
     9901 acttttagca ttttcttcta ttaaaattct tgacagactt tcttttaatc tctttatttg
     9961 ctgtctatac attctagcat gttctctaaa ttttcctaaa ttattttctt tttgtcttgt
    10021 tacatatttt agttgaaata cattattaca ctcacttcta agaactccat ctgtaaagtg
    10081 tattctacaa ccttcatgaa catttctaca attttgtgtc tcaatttttt taatttcacc
    10141 tttactcaaa taagtttttc cataataatg ttctattttt gaatatatct tatttgcagt
    10201 gtcttcatca acatatataa ctcggtctgc tgaattgcta catttagaat catcaaaatc
    10261 ccctacacct tctacaagca tggatgaaga tattgaatct atatcacttt caataatatt
    10321 ttcttcttca tcactataaa gttcttcata catgaaatca gcaaatgtat caaaatttac
    10381 ttctacactt tctacattat tttcatcaat aagactattt tcttctccat ttaatatgta
    10441 ttcgaaataa gttaaatata taacatctaa tgcttttaaa atctccatca tatcttgatt
    10501 ggtatctata tttcttatat cattaactat tcttctagtc atactatcta atgctgggtg
    10561 cttgtccata ttataaagta caaatgtgta tttaatttta tccataatat tatcattgtg
    10621 tattttggta aaacgtgaaa caatatcttt ataagcattt cttcttatgt cttctatccc
    10681 tggcctatct ttaatgacct tttcttcaac tattgtattt aatataactt gaattagacc
    10741 cagtataata tctttgttgt atccttttct tattctattt gtaagatatt ttttaactat
    10801 atcccaatct acatactttc ttctagcacc tgcaattatt gcaaaatata gacctatttc
    10861 ttttgaagag taatcttttt ctgatatatc tatactttca ttgtaatcac cagctattgt
    10921 ccatgataag ttattagctc tattttcaaa ttcataatca tcatatatcc atttcataat
    10981 ctcacctata ttaaatatga cttattggaa atacatctat actctcccat ttattaggta
    11041 ttctagtttt aattacatct ccaaccattt ctttctcata tacatcaaaa gtctttcctg
    11101 taagtcccat attaattgca agagttggtt ttaatcctct tcttatagtc ttaagagatg
    11161 ccataagacc acgtaaatca atagctttac tagatatctc tccattttga gacttcaatt
    11221 gtaaatctaa aaatattcct gcaaagtgta ttaatttttc ttcatcagca tctgaaaatt
    11281 cattttttaa aatcatcatt aacttatctt cttctatagg aggaatatct attaccataa
    11341 atctagatac cagtgcttca ttaagttctt ttgttcctgc atattcataa ttcatagtcc
    11401 caataaatct tgttgctgga tgtagattga ctctctcata cccaggtaca tcaattattc
    11461 ttctatagtc taaagctgaa tgtaccacaa ctatagcatc attttttgcc atattaattt
    11521 catcaaatac tccaaaacct ccattaatag cacactcata aactgagcct cttctaagtt
    11581 taacttcatt gtctataaat gtatctgtac ctataagtgt tgaactgtct gtatttatat
    11641 gaaatgatgt attccactga ggtctattaa atatttcccc tagattatca gcaagtaaat
    11701 tttttcccgt tgcctttggt cccgataata atatattttc ttcttctaaa atagctgcta
    11761 tacacataga caagatatct ttaccataaa aataagtttt agactttgtt actctgtatt
    11821 ctaaatcttt ttctaaacca taataatttc taaaatacag tacatcttca ataagttttt
    11881 catctacccc ttgcaccctt agattatcta ttatatccat atttttaccc cttttatata
    11941 aatagtattt gtttcatttc ttatcaccat tttagcataa aaggatataa gctaaaattg
    12001 attttttaat ctcccccatt ttaagtaaaa ccatgcacca ttttatcact acaaaattaa
    12061 gagtactact tactgatatt tcttgatttt gttcgtctat acgtatataa tatatgctga
    12121 taaattatga ggaaaggatg attgaaatat ggaatatatg tcttgcccag aagtagcaaa
    12181 aaaatggggc atttctgaaa gaagagtaca aaaactatgt gaagaaagtc gcataccagg
    12241 agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc ctgttgatgg
    12301 tagagcgaaa agaagaaagg aagtacaata tgaatagcgt tttgattata gatgatgaca
    12361 aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct gctattactg
    12421 cacattgtgg tgcagaaggt ctacatttgc tcgataaaaa tagacacaca ctctctcttg
    12481 taattttaga cataatgatg ccagatatag acggttttca agttctgaaa aaaattagag
    12541 aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat gataaagttt
    12601 ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata aacgaactta
    12661 tggctcgtgt caattctctt attcgtcggt atactatgtt aaaccctgct tttataacag
    12721 acacagattg cataataata aatggcatga ttattgacaa gacaaaccga attgtttcta
    12781 tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct tttctggctt
    12841 ctaataaggg acgggtattt accaaaaaac agatttatac acaagtatgg gaagaagaat
    12901 atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa aagattgaac
    12961 cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat cgttttaata
    13021 aggaggctta acatgaatac aagtgtctat ctacttttga tattggctct tagcctgatt
    13081 attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac acttataaaa
    13141 gatgctttag aggatataaa aaatggaaac cttaatcgcc gtattttaac agatgaaaac
    13201 gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag ccaatttcaa
    13261 cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag tctctcccat
    13321 gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga aagtaaaatt
    13381 gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc tcattactta
    13441 aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga acaagttttt
    13501 cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga ttggatacct
    13561 gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga gtacttctta
    13621 cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa tattattacc
    13681 catagtagtg gcaacaaaat gacattgaga atttttgaaa acaaagagca agctcaaatt
    13741 ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat ttttgaaagg
    13801 ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt ggcgattgca
    13861 aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc cggtatggga
    13921 acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt cttactctaa
    13981 aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt ataattctaa
    14041 ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt attaaaagtg
    14101 aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa aatttatggg
    14161 aatcaaacag tagttgacaa agtcaatctc catgtaaaaa aaggccgaat atatggtctg
    14221 ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg attgacttca
    14281 atcacgtatg gcgaggtaga tgtatttgga gagaatatta agggatgtga aaaacgagta
    14341 tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct gacaggaacg
    14401 gagaacttgg agatttttgc aaaacttcgt ggtacagccg cccctaattc tgtaaaaaga
    14461 gcattggaga ttgtaggctt accttataaa aataaaaagc tgtttagtaa atattcactt
    14521 ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccgga actgttagtt
    14581 ttagatgagc caaccaatgg acttgaccct attggtattg cggagatgag aaagtttatt
    14641 agggacttga gcgttgagca tggaaagaca attcttatct ccagtcatat tctttcagaa
    14701 atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt ggaagaaagt
    14761 agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt ttcagatatt
    14821 ccaaagacat cacttatttt agagcgtcaa tttaacacga aggattattc cgttcaagat
    14881 gaccacactt tacgccttta taacactact ctggatatgg gtgaaatcaa taaagcactt
    14941 gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt agaggactac
    15001 tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag aatttcttaa
    15061 actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta tgccgttttt
    15121 cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaacaa agttttataa
    15181 atggtctgca tttggttata cactttggat tattttgccc gttgttttgg gtatattttg
    15241 caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt ggattgtacc
    15301 tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt actctatttg
    15361 ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg gatatgtcgt
    15421 atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg gtgtaatcac
    15481 tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat acattctccc
    15541 aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa taaatatgta
    15601 tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca ttccaggtct
    15661 tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta tttggagtat
    15721 tgtatcagta ttgtttgcaa gcattgcact aggaagaaga aagtgaggtg tagataatgt
    15781 ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt tggattacat
    15841 tttttgcatc cattatgata gcagtcatag tttttgcagg agggcaggat gtatataatg
    15901 acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat atagacaatg
    15961 caggttggta tatggacgcg gtacaacctt ggtcaacttt ttttgttctt cctgctgtca
    16021 ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact attaaatccc
    16081 tgcgtcttat tcccataaat gaaacaaaat taaccatagc aaaaatgatt attactttta
    16141 tattcagtat acttctttat ttgttactct ttactataac atttttgact gaatccgttt
    16201 tacatttttc cgatttatcg acaaaactgg ttttaagttg catgaaagag tattttcttg
    16261 atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg cgcatgaaaa
    16321 aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga ctatttgcgg
    16381 gtatgtcaaa tgtcttacaa accttttttc ctattgcagc aatatttaat atttctggat
    16441 actatataac tactggagaa aaaattatgg ggagtatcat aagtttatta ctttgtgcct
    16501 gcctttctgc atttatactg aaagggctaa aacacaatga aaaaaattga aataggtata
    16561 tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt ctttttggca
    16621 tgttacactg ttatactgaa tggaaattca acaatgaccc acctaaaggg gtaatagaaa
    16681 gaataaatga gtggactgat aagattggac tggattagta acttatgtta attatagtga
    16741 atgtaatatt agcaagttat tgtttcgtat tttaatacta taactttaaa tatttggttt
    16801 taatcttccg aacgaatata aaaaaactgt cacagaggca acatattttt gaatatttat
    16861 aaagtggtag ttttgatttt caaagctgcc gccttttctt ctatataagc aaacttatcc
    16921 tcaacaaagt gtaaaacatg gtacatttta agaaaaaagt accacagaca caagcaataa
    16981 aagaaacgcc gagaacctac aaaaacgtag tgcttatgca gatttacgac gccttctatt
    17041 ttctcaaccg tcttaatgac cttgtacaaa agaaattttt ctaatatttt ttataatctt
    17101 atcttgtgat gttttttatg tataaatgac ttaataacaa atccaataaa aattatggat
    17161 attggtataa ttagaacgag gaatttataa tcattataat gtgacattat tcttacccaa
    17221 ctctcagaaa aaatatatcc tatactgatt aatgaagtat tccatatggt tattcccacg
    17281 atagatgata ttacaaaagt tataatattt tgacctgcta tacctgctat aaaagatatg
    17341 tatgttctac ataaagggat taatctacat acacaaacag aaatagaaga gtatttatta
    17401 aaataatctt gagatgcaaa tattcctttc tttgcctttg gacatatctc tattatttta
    17461 tttatgatag atttacctcc ccaatttccc actgtataac aaaatatcga accaagtata
    17521 cctgcaatta tacttaaaat taaaattgtc agaaatccaa aatgatttct tgcagcaatt
    17581 gctccacata aaggtaaaac aacttcgcta ggtaatggaa aacaagcata ctcaatcata
    17641 attaatacaa atacagagat taatccatat tgtaaaataa aactattgat tacagacact
    17701 tacttatcta tctcctttct tatttaaagg ttttaagggt acatattcta aggattctca
    17761 ccctatattt aaagaatcaa tctctttaaa ctattatatg tatttacaca aagcatttat
    17821 aacatacttt aaaaatagta ccttaaaata agaaacttaa taaat
//
LOCUS       DAEPOG010000072        17794 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEPOG010000072 DAEPOG010000000
VERSION     DAEPOG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.072"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KR432_002017"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KR432_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBH0640548.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KR432_002018"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KR432_002018"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0640549.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2903..4210)
                     /locus_tag="KR432_002019"
     CDS             complement(2903..4210)
                     /locus_tag="KR432_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH0640550.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4635..6410)
                     /locus_tag="KR432_002020"
     CDS             complement(4635..6410)
                     /locus_tag="KR432_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640551.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6407..6544)
                     /locus_tag="KR432_002021"
     CDS             complement(6407..6544)
                     /locus_tag="KR432_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640552.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6622..7191)
                     /locus_tag="KR432_002022"
     CDS             complement(6622..7191)
                     /locus_tag="KR432_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0640553.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7375..8010)
                     /locus_tag="KR432_002023"
     CDS             complement(7375..8010)
                     /locus_tag="KR432_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0640554.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8678..9559)
                     /locus_tag="KR432_002024"
     CDS             complement(8678..9559)
                     /locus_tag="KR432_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0640555.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9679..10686)
                     /locus_tag="KR432_002025"
     CDS             complement(9679..10686)
                     /locus_tag="KR432_002025"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBH0640556.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10706..11188)
                     /locus_tag="KR432_002026"
     CDS             complement(10706..11188)
                     /locus_tag="KR432_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0640557.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11206..11673)
                     /locus_tag="KR432_002027"
     CDS             complement(11206..11673)
                     /locus_tag="KR432_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0640558.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11688..12446)
                     /gene="prdD"
                     /locus_tag="KR432_002028"
     CDS             complement(11688..12446)
                     /gene="prdD"
                     /locus_tag="KR432_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBH0640559.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12526..13251)
                     /gene="prdB"
                     /locus_tag="KR432_002029"
     CDS             complement(12526..13251)
                     /gene="prdB"
                     /locus_tag="KR432_002029"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12799..12801),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBH0640560.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13289..13573)
                     /locus_tag="KR432_002030"
     CDS             complement(13289..13573)
                     /locus_tag="KR432_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640561.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13654..15534)
                     /gene="prdA"
                     /locus_tag="KR432_002031"
     CDS             complement(13654..15534)
                     /gene="prdA"
                     /locus_tag="KR432_002031"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBH0640562.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(15963..17723)
                     /gene="prdR"
                     /locus_tag="KR432_002032"
     CDS             complement(15963..17723)
                     /gene="prdR"
                     /locus_tag="KR432_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBH0640563.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT         5093 a         3552 c         2114 g         7035 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
     2641 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
     2701 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
     2761 caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa
     2821 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
     2881 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
     2941 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
     3001 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
     3061 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
     3121 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
     3181 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
     3241 caacaccaca tccacacaca gcagataatc ttcctgtata gttcttaata taaccagtta
     3301 ctagatgaga tatagctaaa gcctttgcta gtttttcatc agattgtggg aattttttat
     3361 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
     3421 taacaggcat ttttacgcct gccattcttg catctgaagc tccagcagtt aacatcattg
     3481 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
     3541 cgacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
     3601 ctccatctag taaaaattct atatctttaa aatcaatttc ttctacattt tttattaatt
     3661 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
     3721 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
     3781 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
     3841 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
     3901 cctcagctat tttaacttct tcttttgtta gagtttctaa cacacttaat ccattttcag
     3961 aatggccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
     4021 gtatacctac gcacatgcca tttttgtaga tactaggact aactaacata cgattttcaa
     4081 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
     4141 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
     4201 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
     4261 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
     4321 tatttttagt cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt
     4381 tatttacata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
     4441 gacaaaaaaa agtctcagaa tgagattcta tatcattttg agacggctca aatgtgttat
     4501 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattatgcaa aacacttata
     4561 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
     4621 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
     4681 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
     4741 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
     4801 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg
     4861 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
     4921 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
     4981 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
     5041 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct ataatataaa
     5101 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
     5161 attcttacat ctattgagac atttgattta cttccaattt tattaagttc cttttcttgt
     5221 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
     5281 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
     5341 aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca
     5401 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
     5461 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
     5521 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
     5581 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
     5641 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
     5701 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacacgattt gtctttctta
     5761 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
     5821 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
     5881 tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc
     5941 ttttctaatt caagctcata agttttaatt tgtgctttta atttattaga tattaagtct
     6001 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt
     6061 tcttttgtaa aggctataag gcctataacg ccatgagaat cgccatcaca tataatagga
     6121 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatacatca
     6181 ctactctttg ggtcatcaat tagtattgtg atctgttctg taagtgattt tttaaaagcc
     6241 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
     6301 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
     6361 atattttgaa tatcagattt tatatattct aaacctatca taaccatcat tccttatttt
     6421 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
     6481 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
     6541 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
     6601 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
     6661 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
     6721 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta acttaacttt
     6781 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
     6841 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
     6901 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
     6961 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
     7021 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
     7081 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
     7141 atatttagct ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca
     7201 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
     7261 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
     7321 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
     7381 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
     7441 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
     7501 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
     7561 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
     7621 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
     7681 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
     7741 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
     7801 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
     7861 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
     7921 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
     7981 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
     8041 tatttctggt atcagcttat ataataaata tatttataag ggatgtattt tattacaaat
     8101 ctttgattgc tgtttaaaag tttttattta gttataaatt tcatttaata tttcactttt
     8161 ataattataa aattattatc tttttgtact acatatacta attaaactac ctatttcagt
     8221 tttgtattaa ttataaaaat cctattatat aattaataag tcatatataa cttaaattta
     8281 ttcactctat ataagcaact tcaacgaatt aaatttattc aaataattca gatgaatatt
     8341 gagtcattat aaacttattt ttcttaatta taagttatat tagacttatt acagcttata
     8401 tttttattaa tctgtattaa tatatgaaca tttattgaac ttattgatta taaaatttaa
     8461 atatttagct ttaaattatg ctttattctt tgaaaataca acgtttttta cattaaatct
     8521 actaaatcac tatatatatt acataattgg catgttaatt gctttatatt aatttagcaa
     8581 ataaaaaact gccctaaaat attaattcaa tattttaaag cagtttataa tctttattat
     8641 tagctgtaat atttaataaa cactaagtag tctttaacta agcagtcttt ttgtttttat
     8701 ttgcagccat tatcattgta gcagaagtat aagctataac tactattact aatgctttta
     8761 atatatccat tggtagagtt tttactatat atgcagctac gaaaactcct attaaaccac
     8821 atatagctat tataactgat gcttttttag tatatgcatc ttcttttata aatttcatac
     8881 ttgcaactgg cattaataat gcacaagaac ccataactat tgggaatgca acttttgcag
     8941 acattcctaa gaaataaacc attgccatac ctggagcata tagacctata ccagctgtca
     9001 ttaaagctcc tagtataaag ttacctatta cacctattac caatttaaca ccagtaagac
     9061 ctaattcatt tcctccacct ggtaatacgt taatccaagg atgacctaat aacattaata
     9121 ttgctgttaa tgctaaagtt acacccataa caagttgtat ttttctctcg tccatcttag
     9181 ctattattcc tgcacctata tatgaaccaa ctgctgctgc cacaaccata gatactaaag
     9241 taacaccatc aactggtatg attgatgtaa atatgaatgc ttctgtaaca actggaatag
     9301 tatgtgctac gtttaatgta ccaggcattt ttttatctgg tatatttaac tttaaagcct
     9361 ttgacattgc tattgttgga gcaaatgacc ctattcctaa tgtatcaaag aagtctgtta
     9421 taaatcctgt tacagcaact tttggagtac tttgttcttc tagtttcccc tctttgtttg
     9481 ctttagctac gtctttacca aacacaactg caaagattgc tgcgaatact acatataatg
     9541 cctttaagat tgctaccata atccatcctc ctattttttt attttttcat atattctgaa
     9601 tcttatagcc taaaagctat agaatagcaa ccgacaagtg gctactattc tattttaatt
     9661 tcctaattta tttattactt atttaagaat aaatccatgt ttaagtgggt cttcttcatc
     9721 tattacaaaa tgattaaatc cagttatata agcagaacca gttattttag gtactacagc
     9781 attgaaatct gcaactttag tttcttcaac tatttcacct ttgaataaag ttcctaatat
     9841 actttcatat acgaattttt ctcctacttt taattcacct ttagcatgta aagtagctaa
     9901 tttagcactt gttccagttc cacatggaga tctgtcaact tgaccttgac caaatataac
     9961 tacatttttg taagtagctt ctggatgagt tggctcatca tatatttcaa ctaagtctac
    10021 agtttttata tgagctaaag ttggatgttg tatttctatt ttctcattta ttatatctct
    10081 aagtttcata gctagttcag ttaatttacc agcattttga ggttcgattt ttaaacctaa
    10141 ttgacttgca tgtattatag caaagaagct tccaccaaat gatatgtcaa atttaacagt
    10201 cccaacacct ggtaagtcaa cttcaacacc ttctttatat aagaaagctg gtacgtttaa
    10261 gaatgatact tctttagctt ttccatctac aactgtaacg tctcctctta ttattcctgc
    10321 tggagcttcc ataactacat gagttacagg ctctactgct ggaactacac ctgtttctat
    10381 agctgctgtc atagctccta ttgtaccatg gccacacatg ttaaggtatc caccaccatc
    10441 catgaagatt attccaaagt cagcatctgg gcaacaaggt tgagtcatta cagaaccaaa
    10501 catatcatta tgtcctcttg gctctaacat tatagcagtt cttaaataat ctagattttc
    10561 ctctaaatat tcttttttct caggcataga atttcctttt atgttaggta ttccacctac
    10621 aactattcta gttgcttcac ctgctgtatg agagtctata gcttgtatac ttctgctaaa
    10681 tttcatatca aattccccct taggtttatt ttgcgtcttt acgcattttt aattttagga
    10741 aagccaatgc ttttttaaca tcaacttcat ccatgtgatt gtctccaact acttctgttt
    10801 ctatgtagcc tgttgtcttt ttataatcaa ttatggtagc tttatccaaa tatggattgg
    10861 taattactaa tccatctgct cccataatag taagacctac tgcaggtata tctgatttta
    10921 caaactctgc tatagcattt gtaaaatcaa tgtgctggtt tcctgccata tctaaagtta
    10981 taatcactcc atcaggtcgc aatgattcaa tccatcttcc aagtctagtt gctaaaaatg
    11041 ttttccattc attgtgctct gatgtcccaa ctacaaccac tcctaacaag tctatgtcag
    11101 aatcattatc tatcaaatct agtaacgggt ctctaaatat atgaagtgat gtgtttttca
    11161 ttgatgtaga aattcccaca aaactcattc ccttacttaa attttttatc catgttttct
    11221 cattttaagt tttaaaaatg ctaaagcttt tttagcatct atttcatttg tgttgttttc
    11281 tccaactact tctgtctcaa taccttcctt tgatttgttc atatcaacta tggtatccat
    11341 atattgattg cttacaacaa acttagcttg agtcccatta aatgtaactc caactacagg
    11401 tatatctctt tttcctattt cctcaaatgt gttagcgtag tcaacatgtg agtttcccca
    11461 accatctgat gaaactatag ctccatctac tctcatggct tccagccaag cggccgttct
    11521 ttgtcccaca aaggttttat tttcatttcc atctggagtt cctactacta ttactccaag
    11581 taaatctaca tccttatcac tttcaactat gtctaaaagt gggtctctaa agtgatgaag
    11641 agatgtttct tttgttgaag gtcctattcc cataatatcc acctctctta agtcatagct
    11701 ctaagggcac catctctgta ttcattagga gatataatca taggtacatt tcccatatct
    11761 ataattgatg ttcctccttc aaatccacta ggttcctttg agaataactg attgtcatac
    11821 atagcacctt gacctgctat ttgttttact atgacaacct tttttgcatt tggtcgaatt
    11881 ttgtcaaaat attcacgaac ttgagtagca tttcttccgt cttccatttt caaagacgct
    11941 cttatttctt gtataaaatc atcacatgct ctgaatgctg caagtggaag tgttctttca
    12001 aaaggtaaac ctccctttaa agttacatct acatggatta tataatcttc aactgatgga
    12061 gttccatatc tcccaaatac catttggtct ttcaaaattc cttctgaaga accaaattca
    12121 tgcatttgat ttccatcttc atctgcacca gttaacatta catatacacc cgttaaagta
    12181 tgtgtgatac cctctcccag tctgcctaaa acttttgttg atattggtat aatgtccata
    12241 atagtattaa tttctctatc ataatctcca ggttttataa tgtctacttt tatgtctgtt
    12301 attaactctt ctctttcttt tatttcatct atacttgata aatctattgt aagtacatct
    12361 ttctttatag aagtttttga accgaaatta acttcattca tatgaaacgg ttttataaca
    12421 agtcttctca ttattttttc ttccataata tccacctttg ttcttaaaac ccctctccta
    12481 accgtgtagg agaagggttc tatattttca aatataaatt acattttaaa cgtgagcttt
    12541 atattcgtat ggtaatggaa ctatcttacc agcagatggt attgcaacta attgctctaa
    12601 tgtatctctt aatatatgtg tttgcatttc tatattatgt ggttcaccag cattagcacc
    12661 cattggaact aatggagcaa ctgctctagg agttccgttt tgtctaacta ctggaggaag
    12721 agctgctatt attattgtag gtattccagc ttcctctatt gctctctgca cgatcacggc
    12781 agttctatgg caagtacctc agccagctgt cataactaca ccgtctactc cctcttcctt
    12841 aagtttagca gcgatagcag gaccagtttc ttcagtgaag acatgttggt ttcctccacc
    12901 acccatgaat gcatagtgca taggagctac tcctttgata aatccttctg cagctaattc
    12961 atgtaatcta tctataggga acatacagtt tatatctttg tttacatctc cattgtcata
    13021 tccaccatgt gataccatca tttcatcaac tgtagctgta ttaggtatag ctctaaatgt
    13081 agtatctcct gctagattaa atctcttgtc agattttaaa tgaacaccag ctgctgtagc
    13141 taaagctata gtcatatctt ttaattcttt agttacagga gtccaaactg gaggaggtgt
    13201 tattggaacg aatatttcag attgaagtcc ttgtactgtt gtaaggctca tatatttcct
    13261 ctcccttcta ttttcaaacg attatttatt atttttcttc ttgttttgat tgcatttctg
    13321 cttgtttttc tttttctctc ctaatatttt ctacatactc ttgattaggc tctggactta
    13381 atacctcagg atagcttagc atgaatccaa cttcttgacc tatcttaagt tcgtagtcag
    13441 ttataagcat tctattaggt attttaaatt gtccaagtct accttttagg tctatagtca
    13501 ctccaccacc tttgatgtca actataatac cttcataaaa agtctctgat gagatatatt
    13561 ttaacctatc cattataatt ctacctctct atactgtttt aaacttggat ctaaatttat
    13621 gttattgtct aaatcttagt tagtatctaa attttagttt tcgtcttttt cgtatatttc
    13681 tcttctcttc ttactcattg gtagagattg ctcattctct tctaatacta ttttttctcc
    13741 tgtaactttt tctatagctt ctatgttatt taatttaaca tttggattcc attttctttc
    13801 aggagcttta acttctacac cttctatagc atttttaagc atagccatta ttctaacagc
    13861 atcttctgga gctaaagtgt tgttagataa tatttcattc tctatacctt gcttagactt
    13921 attgttgtct accatgtgag tcatgtattt attaccaaca actagagcac cttgaacagc
    13981 tgagaaactt acaccaacta ctggtatacc tctcatacct atttgctcta tgtgagatgc
    14041 gaaatctata tggttgtttc cgaaaccttc agtagttact acagctccat caacttccat
    14101 agcttcaact aacataccta atctcttaga tacatagaat ttctcagcat ttacttgtgg
    14161 agaaccaaca aatacaacac ctactaaatc tatttcttcg tcttccatag ctcttattac
    14221 taatggctct ctccagtaat gtcttgacat ttcttttgat gcaggtccta tacaagttaa
    14281 tgcatggata ccaccatcta atacttcaag tggagataat acaactggca tgtttcctaa
    14341 gtcaacgttt ggttgagctc ctaatgtacc aactggctca actggtaata ttaggttatc
    14401 atgcattgca ccttgtccca ttatttcttt tataacaaca actttctttt taccttctct
    14461 tctgtattgc tctattactt cttcatcaac tactaaagag ttgtcaactt tctttaatgc
    14521 ttctcttatt tcttgagtta catagtcaaa tgcacggtga gcagctaaag gtcctggtct
    14581 ttccatgtta gttcctgctt ttattgttac ttgacctttg atgaatattt cacctttgtc
    14641 agcagcacct ggtctacccc acattatagt agtatttaac tcaccttctg aagaaccaaa
    14701 ttcacctatt tgaactccat cttcatctgt accagttaat accattacag ctccatctat
    14761 aactctagtt atacctgaac ctaattcgcc ttcttcttta gtagcgattg gttgtatatc
    14821 taatacagct tcactgtaag tttcatattt atcaggagtt attatttcta acttcatatc
    14881 tacaactaac tcttgagttt cattagcttc tttacatatt tcttctgcgt ttctgatgta
    14941 taaagtagtt ccttctattt tagtttcatc agcaaattca actttatcta ttttaaagtg
    15001 ttttttagtt aagcttctta aaagtttctt ttcttcaact tttggagcta cttctgctgc
    15061 tgcacttgcc actggagcag caacgtttgc tactggagca gcaactcctg cttgaccagc
    15121 tactgataaa ggtatctcta agcttatgtt cttaccttca cctatgtgta ttttaactgt
    15181 ttcacctgca actcctgtgc ttactggaac tactgctgct tctgatactg gagctgcttc
    15241 ttctactgtt tcttctacta cttcttcttt agcctctcct gctttgtatc cttctacgtt
    15301 atctgtagtc attggagaaa gtgcatctaa agtttctttt aacttagctc ctagtacttg
    15361 acctatagtt aatccatttt ctggtattgt taataatcca gaatcctcta agtctgcaaa
    15421 tattgctgga tcttctaagt tttctggcgc tataattgtt cccgcttcaa atctacaaca
    15481 acaaactgct gggtcatttg catgggcttg agctgtttct aaagttattg acatagtata
    15541 aaatacctcc ttacattatt ttcttattta ttattttaaa tctcaattcc tcaacaggta
    15601 accccttaac tgagaaatta agtaaattaa aacctacata atttgttatg attaatatta
    15661 tttttttctc actcaaagtc actttatatt ataagcaatt cctatgccaa cttattaaca
    15721 aaaagatttt ttctataata gattcttcat ctatcgctat ttttagccaa atattatata
    15781 aatatttatt taaaatatgt tattttttgt atttgtaaac attttctcaa acaaaaaaag
    15841 tgtcaattaa tcaacacttt ttcatttttt ttaacacatc tatatacaca acacttatac
    15901 aagtgttggc tttactaggt tttaaattaa agtttatcat tttgtcaaaa tttagtcata
    15961 tgtcatattt tagacacatc tattttgtat tggtctatct tataatacaa agttgttctg
    16021 ggtatattta atatcttagc tgctttagct ttatttccat tagacatttt aagtgccttg
    16081 gttatattct ttatttcaat tttttgagtt gccttcgtta aatctaaggg atactcttct
    16141 tcttcagtac tattatttgt agaatccatc atataacttg gtacatcatc tacatctatt
    16201 ttagaatttt gtgacaatac aacaatattt tcaatcgtat tttttaattc tctaatatta
    16261 cctttccatc tataattctg aaatatatct attacatctt tgctaattgt aagaacaggc
    16321 ttattatttt gtttacatat ctcctctaaa aagctatgaa ccaataatcc tatgtcttct
    16381 tttctctctc taagtggagg aatctttatt tcaacaacat ttaatctata gtataaatct
    16441 tctctaaatt tttctgcctt taccatttta ctcaaatctt tattggtagc tgatattatt
    16501 ctaggattta tttttattgt ggtatctcca ccaactcttc ttatttcttt ttcctgtaat
    16561 actctaagca actttgcttg catactaaga ggtaaatcgg caatttcatc taaaaataca
    16621 gttccatctt ttgcaagttc aaatatacct attcgaccct ttttacttgc tcctgtaaat
    16681 gcacctgatt catacccaaa aaactcactt tcaaataact catttggtat ggcactacag
    16741 ttaacaggta taaattgacc ttttctctca ctataatcat gaatagctct tgcaaatact
    16801 tctttacccg taccactttc accccaaata aatatgctac tatttgttct agccacttgt
    16861 tttgcaatcg ctttcgattt ttccaattta taacttttac ctaaaatctt atcaaaacta
    16921 cctttagata aatttttgac ttcgttcttt agatatttta atgtatcatt tactttttca
    16981 agttcaagag ctaatatctt agcttctgta acatcaactt ctgtacatac aacaccgata
    17041 aattcttctt tataaaatat aggatttgca tgaactaatg catacatatt ttctttttta
    17101 tctgtaaaat acaagtcact cattccaact ttactattta gtactttttc tgaaatagta
    17161 ttttccaaaa attcattcat cggtcgacca actatatctt cagactttat atcatacctt
    17221 tcctccataa acttattcca caagataaca acaccttcgt tatttatagc acaaattcct
    17281 tctttaattt gacctataat atacttcaaa gtcaatccat aatcttctaa atgcatatat
    17341 agatagtccc taatgtgctc ttgttttaga actccaataa ttttattatc tctaagaaca
    17401 ggaagaatcc ctatattttt taatatcatt atatctctac attcatctaa ggttaaacct
    17461 tcgttgacat atattacatc cttcttcatt ataagtttta ctggttgacc ttcatacttt
    17521 ttatgcatct cataaagatt atgtatatcc gtcatggata taatgccttt taattggtca
    17581 gatgaatcta ttaccattag agtttttgtg ttgcttttta tcatttcttt tattgcatct
    17641 tcaattcttg tgtcttcgtc tatagttgta aattttgtat ccattacctc ttctactttt
    17701 tttacttctg gtatcgaaaa cataaagtcc ccccctttat agtagtacct catcaaaact
    17761 tatttatata tatttattct agcatattat aata
//
LOCUS       DAEPOG010000073        17770 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEPOG010000073 DAEPOG010000000
VERSION     DAEPOG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.073"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(350..1246)
                     /locus_tag="KR432_002033"
     CDS             complement(350..1246)
                     /locus_tag="KR432_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0640564.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1388..2815
                     /locus_tag="KR432_002034"
     CDS             1388..2815
                     /locus_tag="KR432_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0640565.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3012..5606)
                     /gene="clpB"
                     /locus_tag="KR432_002035"
     CDS             complement(3012..5606)
                     /gene="clpB"
                     /locus_tag="KR432_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH0640566.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5643..6239)
                     /locus_tag="KR432_002036"
     CDS             complement(5643..6239)
                     /locus_tag="KR432_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640567.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6256..6660)
                     /locus_tag="KR432_002037"
     CDS             complement(6256..6660)
                     /locus_tag="KR432_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBH0640568.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7027..7401
                     /locus_tag="KR432_002038"
     CDS             7027..7401
                     /locus_tag="KR432_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0640569.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7398..8261
                     /locus_tag="KR432_002039"
     CDS             7398..8261
                     /locus_tag="KR432_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640570.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8261..8899
                     /locus_tag="KR432_002040"
     CDS             8261..8899
                     /locus_tag="KR432_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0640571.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            9392..9805
                     /locus_tag="KR432_002041"
     CDS             9392..9805
                     /locus_tag="KR432_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0640572.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
     gene            9996..11213
                     /locus_tag="KR432_002042"
     CDS             9996..11213
                     /locus_tag="KR432_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBH0640573.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            11375..12073
                     /locus_tag="KR432_002043"
     CDS             11375..12073
                     /locus_tag="KR432_002043"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBH0640574.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(12293..12910)
                     /locus_tag="KR432_002044"
     CDS             complement(12293..12910)
                     /locus_tag="KR432_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH0640575.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(13037..14059)
                     /gene="argF"
                     /locus_tag="KR432_002045"
     CDS             complement(13037..14059)
                     /gene="argF"
                     /locus_tag="KR432_002045"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH0640576.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(14168..15355)
                     /locus_tag="KR432_002046"
     CDS             complement(14168..15355)
                     /locus_tag="KR432_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH0640577.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(15385..16245)
                     /gene="argB"
                     /locus_tag="KR432_002047"
     CDS             complement(15385..16245)
                     /gene="argB"
                     /locus_tag="KR432_002047"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH0640578.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            complement(16260..17477)
                     /gene="argJ"
                     /locus_tag="KR432_002048"
     CDS             complement(16260..17477)
                     /gene="argJ"
                     /locus_tag="KR432_002048"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBH0640579.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(17519..>17770)
                     /gene="argC"
                     /locus_tag="KR432_002049"
     CDS             complement(17519..>17770)
                     /gene="argC"
                     /locus_tag="KR432_002049"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH0640580.1"
                     /translation="KLTDYYEFKEFIEVLPLDKVASLKNVRFSNKCVISLHENGDTLI
                     ICSAIDNMIKGAAGQAIQNMNIIFGIEENTGLKNIAPSF"
BASE COUNT         5943 a         2755 c         2013 g         7059 t
ORIGIN      
        1 tttaattctt taggatggat ttaaattttc tataaaaatc atactatttt agctgtttac
       61 ttgcttattt gatagtttta tttctttgat taaatatctt tcagaatcta agccagatgt
      121 ctcaaaaaaa ctgatttata atataatcat tttttctgct attcttattt ttccaatttt
      181 agtttcatat taaaatatac ttttcataga cactacattc tttcatactc attttcacat
      241 ttaactatac aagtagtctt actcatttat gtactatact ttattaatat ttattaagaa
      301 aaactcaaag atttaaaaat atatattttc tataaagtat atttatgaat tatttcatat
      361 cttcttttaa aataacatta ctaacttctc tatgttcatt tctatactta gttggagata
      421 tattataatt ccttttaaat aagcgactaa aataatttat gctatcaaag ccacatctca
      481 tagcaatttc tgtcatattt aaatctgttt cctttaaata atatatagct ttatcaagcc
      541 tgattccatt tataaaatcg attgtagtct tacctgttat ttgcttaaat gttctacaaa
      601 agtgatgtgc actcaagttc acacgactag caagttcttt taatgtaatt ttatccatat
      661 atttttcttc tatcaagtca aagatacatt caaatttttc taaagaatac tttcttttta
      721 taaattcatc ttcagaatat attttatcaa tatgacttct taataagagt actattaatt
      781 gatagatatt agcttttata gataactcat atccaacttc tttattgtga tattcatcaa
      841 gtatagttgt aatacatttt aaaatcagaa aatcattttg aattaaatta ttaaatacaa
      901 tatgatttaa agccaatggt gctaagtact tagtttgtaa caaatcaact tgattactaa
      961 aaaggaatgt gggctcaata cgaatactat agaattttaa attatctgat aagctttcta
     1021 aataatgtaa ttcattactg tttgctatta caatatttcc aggcactacc ttaaaattat
     1081 ccaatccaca ttcaagatat gctataccat caataaaata atatatttgt agatgctcat
     1141 gccaatgtct tttaaagttt gctccttttt taatgtcatc ctttaaaaaa atatctataa
     1201 agttttcttg acctttccta ttaaaatatg tatttttatc cttcattatt cccccctcat
     1261 caaaatagtg ctaattataa gcaatatagt tattgtattc attatttcat atcgctatta
     1321 tataaaatgt aactatcatt gtaaacatta acataatgcc agtcattata ataaggaggt
     1381 aataaaaatg acaaactctt ttaaaaaaga tgataataga aaaaaaatca cagttttatt
     1441 tgtagtcatt gccatgactt ttatggcaac attagattcg agtattataa atgttgctct
     1501 cccagttctt gcaagcaaac taaatgtatc tctagcatct attgaatggg taatagcaag
     1561 ttactcaatt attatttgtt caacactatt attttttgga agacttggag atattattgg
     1621 taaatcaaga gtattccaag taggtactat tctctttaca tcagcttcat tactatgtgg
     1681 attaagtaat tctttaactt tacttatagt ttgccgcttt attcaaggaa taggcgcttc
     1741 tgcctacatg gcaaacaatc atggaattat cacagaacta tttccaaaag aaagcagagg
     1801 aaaagcccta gggattctag ttactgcagt tgctattgga aatatggttg gaccatctgt
     1861 gggaggattt attttgtcaa tatttgattg gaatgttata ttttttataa atattccaat
     1921 cggtcttatt gtaatctttt taaatacaaa atttcttcct aatagtaaaa aatcttcaga
     1981 aaatatggat aaaactggtg ctatacttca atttttagga acaacattat tttttagtgc
     2041 tctaattagt gctcaacaaa caggattact aaatccatat atattaattg cactattatt
     2101 atcaattatt tttattatat tatttttgat attagaaaaa aaacatcctc aaccgcttct
     2161 tgatcttgaa atttttagaa attttaaatt ttcattgaat ttaatttgtg ctcttacatc
     2221 attcatttgt attgcatcat caagtatttt aattccattt tacttacaaa gtactatgaa
     2281 acttccccct attcaagcag gtttatttat gatactatca cctttgattt tagccatttt
     2341 ttcacctata tttgggaata tatcagataa aatcaagtca gaaaaaatta ttttaattgg
     2401 tcttctcgtt atgagttttg gattctttct aatgtcaaga ttgaaagaaa gttctgccct
     2461 tattttattt gtaatttata tatcgataat atcaatagga caagctatat ttcaacctgc
     2521 taacaatgct ttgataatgt caagctgttc tagaagtaag ctaggagttg taggaagcat
     2581 aaattccctt gtaagaaatt taggacaagt tataggtatt actatttcta caacactttt
     2641 atataatttt atgagtatta aagctggata tagggtcaat gattatgtga ttaataatga
     2701 taaaatattt gtatttggaa tgagaaatgt ttatattatt gttacacttg tttgcttaat
     2761 aggtgcaata ttaattggct tttatttatt taaatataat aaaaatgaac agtagttttt
     2821 tataattata ttcaaattat gattatatta taagaaactt actaaaatac ttcctttgag
     2881 ttaaatttaa tttggacata ttgacttaaa aatgttttat taatttgata aaatatttct
     2941 tttcctatag tttataaata aaaatcagct gagcaaaata tttagtatat tatgctcaac
     3001 tgattctttt attatttaac taaaacttca atattttcat cttttccatc tataactata
     3061 gtacatccat tatacacatc tccagcaata agtttctttg caattatagt ttctaatgta
     3121 tttcctatat atctcttaag aggtcttgct ccatatacag ggtcatatcc ttctctcacc
     3181 ataacatctt ttgctgaatt tgtaacttca atagtaatat ctttttcttt taatctattt
     3241 tttaaatctt tcataaatat atctataatt ttcttgatat tttcttggtc taaaggctta
     3301 aacattataa tatcatctac tctgtttaaa aactcaggtt taaatctatg cttcatttca
     3361 ttcattacta aattgttagt agtctctgtt atatttcctc ctgcttctaa tagataatta
     3421 cttcctatat tagaagtcat tattataaga gtatttttaa agtctactgt cttaccttta
     3481 ttatcagtaa gtctaccatc atctaatatc tgtaagaaca tgttaaatac atcctcatgt
     3541 gctttttcta tctcatcaaa tagtattaca gaataaggag cacgtctaac tgcttctgta
     3601 agttgaccac cctcttcata tcccacgtac cctggaggag gtccaactag tcttgataca
     3661 gcatgttttt ccatgtattc agacatatct attcttatta tattatcgtc actatcgaat
     3721 aaatttctag caagagtttt tgcaagttca gttttaccaa cacctgtagg gcctaagaaa
     3781 ataaatgaac caattggttt tctttcatct tttaatcctg ctcttgcacg tattactgca
     3841 tttgaaactg ctgtaactgc ttcatcttgt ccaataactc ttttatgaag ttcatcttct
     3901 agtttaagta atttttctct ttctccttca actagtttag ttactggtat acctgtccac
     3961 tttgatacaa tgctagatat ttcttcttca gtaacttctt cttttaaaag agaattttca
     4021 gaaccatcat tcatattttc ttcgtactgc tttatttgtt cttctaactt aggtatttca
     4081 ccatatttaa cctctgctgc tttgttgaaa tcatattctc tttcatactt ttcagcttta
     4141 cctttagctt cgtctaattg agcctttaag tttttaatat ctaatatttg actcttttct
     4201 ttttcatatt ttgctgtcat ttcatcattt ttagatttta actctgctat ttctttttga
     4261 atatcatcca atcttgattt gcttttatca tcattttctt taagtaatgc ttctctttca
     4321 gtttctaatg taaataactt tcttctaaca acatctaatt ctgttggcaa tgaatctatt
     4381 tcactacgaa tcatagctcc agcttcatca attaaatcaa ttgctttatc tggtaaaaat
     4441 ctatcttgta tatatctatg agaaagtttt gctgctgcta caatagcatt atcatgtatt
     4501 cttactccat gatgtatctc aaatctttct tttaaacctc taagtataga tattgtgtct
     4561 tcaacagtag gttcttctgc aattacaggc tgaaaacgtc tttcaagagc cttatctttt
     4621 tctatatact ttctatattc atcaaaagta gttgcaccaa tacagttcaa ttcacctctt
     4681 gcaagcatag gtttaataag attacctgca tccattgaac cttctgtttt accagctcca
     4741 acgatagtat gaatctcatc tataaacagt ataatctttc catcagaact ttgaacttct
     4801 tttaaaactg cctttaatct ttcttcaaat tcacctctat attttgcacc agcaattaaa
     4861 gctcccatat caagtgagaa tacaatctta tcttttaaac cttctgggac atctcctctt
     4921 actattctct ctgctaaccc ttctacaatt gcagttttac ctacacctgg ctcaccaatc
     4981 aatacaggat tattttttgt tcttcttgat aaaattctta taatccttct tatttcttca
     5041 tctctcccaa ttacagggtc taatttattt ttcttggcta aatctactaa gtttgttcca
     5101 tatctagcaa gtgcatcata tgtgccttct gggtcttgtg tttctactct ttgactacct
     5161 ctcacttgtg ataaaatatt taaaaagtca ttcttattta tattatattg ttttaatatt
     5221 ttaccaacag ctgattttga ttctgtttcc atcattgcaa gcataacatg ctctacactt
     5281 atatatgaat ctttaaattc tttagaaata ctctctgcct ttataagcac ctcatttatt
     5341 tttctagtag ctgatactcc ttgtgagtca gcaccttctc catatacttt tggaagctta
     5401 tcaatttcaa agtttacagt atttctcaaa gaatcaattg atatattcat tttttcgatt
     5461 atatttggta tcaatccatc ctcttgatta ataagtgctg agaaaagatg aattacatct
     5521 acttgttggt tatgattttt tactgcttcg ttataagcct catttaaact tttttgaact
     5581 cttaaagtca ttttttctac gtccatataa atccctccat tttaatatta tatccaaata
     5641 aattaaattc tctcatttat aattattgat tttctaagct ctaaaagact atcaagtaaa
     5701 ttctcgtttt tttgtatatc gaaactcaat gttaaattaa atgttccatc tgaattaaaa
     5761 aaatcgaata cttcttttga ttttgctatt aaatacaagt cttcatctat aagtcttttt
     5821 tcacctatta ataaatactg acatttatct ataaaactct ttttaaaatc aatagtgtat
     5881 agattttcta aaatattata ttgataaata gcaatattat taaacacttt taatatgttt
     5941 gaatttacaa aattgtaaat ttgattattg ttatacagtg atatttgttc gtatatatat
     6001 tccctaaata aaggatgaat gttttgctta agttttaaag taacttcagc tggtatttca
     6061 aaataaagct tccagtactt agagttattt ttttctaaaa taaaagctac tttgttcaaa
     6121 ctggtagaac taatctcctt tagtgtacaa ttaaaattat agtgtctcaa actagttttt
     6181 tcattctcaa atacatagtc aaaccactgt agaacatttc tatcaagttt taaactcata
     6241 taaaaacctt cttccttatt tactaacctt attaggtctt tttatctgat tatattctgc
     6301 taataaactt tctttatcag ctctactacc tttatatgct atatgtggat ttatcatata
     6361 ctcaccattt ttcactcttt gtaaaaaatc catttttttc aactccgtaa acaatgaaga
     6421 taaagaagtt tttggatatt caaattcaca agataattca tataatgttt ttctaatctt
     6481 attattttta tccatagagt ttattaattt taatggaatt aaatccctct tagtatacat
     6541 tccttctact actttaatat aattcttaaa acaaatattc atatacttac ttttattcat
     6601 aactaaactt ttgctctctg tatattcctg atttaaatcc ttttttaatg tcttatccaa
     6661 attaaatatc tcttctcact ctccttatct ctaattgata atataaatat attacaaaaa
     6721 aatatgtatg tcaaaactga aaatgcaaaa agtttacctt ataagtaaac ttttgtttag
     6781 ctataagcta aactttatta tttttttaat ttagtatttc caatttattg gcaggatttt
     6841 tgaataatat ttataaattt tttaaaaaaa tttaaatcta tttaatatgg cagcctttca
     6901 taaaatacat tacatttatt aaatgtataa tattggtaat tagttttttc taaaatcagt
     6961 tgacatatta tatatatatt tatataatgt gtatatacta tatatacaat ttaaggaggt
     7021 gacttattga atatagttat tagtaattcg agtgggaagc caatatatga acagattact
     7081 acacaaatta aaagtatgat tataagcgga gagttaccag aaggctcagc tcttccaagt
     7141 atgagactat tggcaaagga acttagaata agtgtaatta ctacaaaacg agcatatagc
     7201 gatttagaac gagacggatt tattgagaca gttactggta aaggcagttt tgtatctagt
     7261 aaaaacatgg attttgtcaa agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa
     7321 gcagttgata cagcaaaaag tagtgatatt acatatgacg aattaaaaga tattttattt
     7381 ttattatata aaggagaatg ataagtatgg atgcaataac tataaaaaat ttaaataaaa
     7441 cttataaaga ttttagccta caagatatat ctttttcagt accaaaaggg agtgtaatgg
     7501 gatttgtagg tgaaaatggt gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa
     7561 tatcttatga tagtggaaat atagaaatat ttggtcttga tagtaaaaaa aatgaaaaag
     7621 aaattaaaga gcaaattggg gttgtatttg agggaagcaa ctttcatgaa aatttaaaca
     7681 ctgaccatgt ttcaaaaatc atgagtaaaa tctataaaaa ttggaatgac acattattta
     7741 aagactactt aaaaaaattg agagtaccag acaataaatt aattaaggag ttttcaaagg
     7801 ggaataagat gaaacttagt attgctgtag ccttatctca taaaccaaaa ttattaatac
     7861 tagatgaggc aactagttca cttgacccta tcgttcgtga agaaatacta gatatatttt
     7921 tagattttat acaagatgaa gaacactcta ttatcttatc ttcacatatt acaagtgatt
     7981 tagataagat agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa
     8041 ataaggatga attaattgat actatgggag ttttaaaatg taaacctagt gattttgata
     8101 acctttcaag agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc
     8161 ttaaagataa acatagattt atatcaagaa atccaaattg tattgttgat aatacgtcta
     8221 tagaagaaat tatgttattt tatgtgagag gtgataaata atgaaaggtt taattttaaa
     8281 agatttactt aatttgaaag gaaatgtaaa gtttatactt ttattcatca ttatgtttgg
     8341 gtttatgtct tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt
     8401 atgtactaca atgattgttt caacattttc atatgatgat ttaaataaat gggatagtta
     8461 tgtacttaca atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt
     8521 aatatttagc tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt
     8581 taaaaataca cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc
     8641 agtatgtttt ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg
     8701 tttattaatg attttatgct ttttagttcc aacactagct ttattagttt ttaaaagtat
     8761 tttagaaaat attagttcac ctatttctat agaaattata cttaatacac tagtatattc
     8821 tcttccattt gttgctattt tactatttgt gatttcatat tttatatcat caaaaatcta
     8881 tagtaaaaaa gaagtataaa atatactata catgtctctg tatttaagta ttttaaataa
     8941 aatactatca agtttatttt ggtagtattt tattttttta ttattaatat ctactaggta
     9001 tttattgttt tattacttat atttaataag actatttact actttctaat cttatttata
     9061 taatttattc attttataaa ttgaactact aatgatatta ttgaacatag caatagaata
     9121 acatctaaaa tagtttttat gtattaaatt aacttatttt aatattcaac aatttaggtt
     9181 tactaaattt attgatgatt tctttttata taacttaaac tacattttta ttttaaaaaa
     9241 tttaatatca acaatataaa attaagaata agtattatta gtttgacatt agcataaatg
     9301 cctattagaa taatgttaaa ctatattctt tctactgatt ttattaaaaa ataccaatat
     9361 acaaaagatt atacaaggag aaagttcaaa tatggaatta gaaattacag aaaaaataga
     9421 aaaacaagat gaagatacta tttttcaagg attattaaaa tataatcttg ctagacttga
     9481 agataaaaac ccagtggatt taggaatata tattcgagat aaagcaggta aaattgttgc
     9541 aggttttata ggtgttactc atggtaattg gctttctata aaatacttat gggttagtga
     9601 gaagcttaga tataaaggta ctggaagtca acttttatat aagactgaaa aaattgctaa
     9661 agaacgaggt tgtaaatatg tttttttaga tacattcagt tttcaagcac ctaaatttta
     9721 tgagaaattt ggataccata aagtattttc acttgaaaat tatcctctaa ctggaaagag
     9781 atattattat agaaaaatac tataataata aaaatcatat tttatatttt taacttaaaa
     9841 cttctgctct tcgtctacag actttatgtt tttatatcat tattaatact aaaatagtat
     9901 tttactatac atacaaaatt atttgaaagt tcaaaaatta taagtataaa agctaatctt
     9961 agcttaaata aaaattatat ttttaggagg taaatatgtt aggaaaaaaa tgtatggatt
    10021 atctacaaac attaggaaaa ataagttcaa caacaaatgg tcttacaaga cttatcttga
    10081 ctcaagaaca taaaaaatct attgatttaa ttagttcatg gatggaaggt ttaaatcttg
    10141 atattgaaat tgatgacatt ggaaacgtta taggtacata taaatcaagc tttccaaatg
    10201 ctcccacatt agtagttgca tctcatcaag atagtgtaaa gtgtggaggt atatttgatg
    10261 gtatgttagg tataatagtg cctctagttg gtcttgaaga agcaaaacac aataataggt
    10321 cttatccttt caatattaag ctaattgctt tcgctgaaga ggaaggaact agatttgaaa
    10381 cttctttaat gggaagtaaa gtttttgctg gtacttttaa agaggaactt cttaaatctg
    10441 ttgatgaaaa tggaattaca cttgaagaag ctgtaacaaa gtttggattt aatacaaaaa
    10501 acttaacaaa tttgcatcct agaaaagatg tagatgcata tttagaattt catatcgagc
    10561 agggtcctgt tttagaaaat gaatctcttc ctgctggaat tgtatcaagt attacaggat
    10621 ttaaaagttt taagatatca gtaaatggta agtctggaca tgctggtaca cttccaatga
    10681 atatgagact tgatgctgga tgttgtgcct gtgaatgtgt attagctatc gaaaaagtag
    10741 ctaaaacgac tgctgatttg gttgctactg taggaaagat gaatttctat ccatcttcat
    10801 caaatgtggt acctgaaaga gctgaattta cactcgatgt acgctcttgt tcacaagaaa
    10861 ttcttgataa ttctgtagaa aagatattta atgaaatatc acatatatgt gaaaatagaa
    10921 aacttaatta tacaagtgaa cttgcttttg aaaatgttcc tgtaccatgt tctaataaaa
    10981 taacaaaaat catagaaaaa agttttatag atttaaatct taatcctttt tatatttata
    11041 gtggtgctgg ccatgatgct caggaaatgg ataatataac tgatattggt atggtattta
    11101 taagatgtgc tgggggcgta agtcataatc ctaatgaaag tgtttctgta gatgacttgg
    11161 atactgctgt aaaaatattt ttaaaaatac tagataatct tgatttaaaa taatttttat
    11221 ataagaaagt atattaaaat atataatcta aaaaatcact ttatatttat attatacaag
    11281 tgtacaatat aatctattat aaaatataat tttatgcttt tatagatttt tattttatac
    11341 ataaatatat ttatatataa aggagataat ttttatgaat gaaaaattaa ttggtatttt
    11401 ggctggaatg ggtccaaggt caacagcccc ttttgttgat ttaattattg atgagtgtca
    11461 atctcaatat ggagcaaaat atgatgatga atttccaaag atgatgatat attcattacc
    11521 taccccattt tatattgacc gtccaataaa tcatgaactt atgaaagaaa ctataattga
    11581 tggattacaa aagcttgaat caactggtgt aaattttatt gcaatgccat gcaattcagc
    11641 acatatatac tttaaagaat taaaagagtc tattaatata ccaatattaa atattgtaga
    11701 agaaacagtt aaaaaattac ctactatctc acaaaaagta actctttttt cgactagttc
    11761 aacttttgag tctacaattt accagaatgg tataatacat aatggacatg aatttatatt
    11821 taaagatgaa tggcaaataa aattaaacaa tcttattcaa aatataaaaa tggataaaga
    11881 aaatccacac aatattgata tttggaataa acttatcgaa gatgttaaaa atgaatctat
    11941 agaaaatata gtaattgctt gtactgattt aaatgtagtt ttagaaaaat catatccttc
    12001 aataaatatt gttgattcat caaaatgctt ggctgaatca gttgtcaata aatatctaaa
    12061 attagttaaa taaatttttc atgcactata ttataaaaaa tatatgttat aagtttattt
    12121 taaatagtat agacttataa catatatcat agtttcttga caatatagaa attaaccatg
    12181 tatttcatta tattaataaa aaggatacca aaaatggtat ccttcataaa tatttatatg
    12241 tttatttata aaaataatca tatatttatt actttactac acctaaacaa tattattcaa
    12301 gtgtattcat agagttagaa aattcttgtc ctgctgtaac aagattagat aacaatacat
    12361 cagctactgc atcatttact gaacttatta tccatttatt atccttttta gaacattcaa
    12421 actctacagt ttttgtaaca taagtctctt taacgctttt ttgtttttct ttcataattg
    12481 atactaacat ttcagttgtt ttctcatcag ttaagtcttg tcctgaaaaa ctcataccaa
    12541 tcatctttgt aaatgcctca gctactattt cttgaagtaa tggtgttgaa tctataaatt
    12601 tacattctac tttgattgta gccttatcac ctttaacctc gctgtctttt attgtatacg
    12661 tgattttaga tgcattctgt tttagataat ctaaaacata ttgggcattt gggtcttttg
    12721 tatctaattc ctttaatata tccttatatt tttcgtcatt attttccatt accttattca
    12781 tgccttcaaa gtcaaacttt ttcgcactgt taaaaaaact atcaattgta tcttctggtt
    12841 ttgcacttga acatgctgtt aaagatattg ctaataaaaa agttgttaaa gctaatatga
    12901 tttttttcat atctcaaatc ctccctctta atttgttgat acataagtta ttttaacata
    12961 taatgagagt ttttgaataa tatatttaaa atattttata aattttcatt aaagtaaagt
    13021 ctatattaaa acatatctat attaaattat tgttcatatt tacatcagtc atagtagaaa
    13081 ccataacagc ttttattgta tgcattctat tttcagcttc ttcaaatacc tttgaatatt
    13141 ttgaattaaa tacctcgttt gtaacctcta attctgataa tccgaacttt tcataaatct
    13201 tctgagcaac cttagtttct aaatcatgaa atgctggaag acagtgtaaa aatataacat
    13261 tttcattttc agttttcttt atcatatcca tatttacttg ataatcttta agttgattta
    13321 ttcttgcttc atattgctcc tcttctccca tagaaaccca tacatctgta tatataacat
    13381 ctgcaccttt aacatcatca atattttcag tcaacattat atctcctttg gatttcttgg
    13441 ctatttcctt catttcctta accaattctt catttggcca aagttcctta ggtgctagtg
    13501 ctacgaaatg tattcccatt ttagcacatc ctatcatcag tgagtttccc atattatttc
    13561 tagcatcacc aacatataca aatttaactt tattcaaatc tttatctaca ttttcaatta
    13621 ctgttaaaaa atctgccagt atttgtgttg gatgatattc atctgtaagt ccattccaaa
    13681 caggtacacc agaatatttt gctagacttt caactgtttc ttgtttaaag cctctaaatt
    13741 ctattccatc atagaatctt cctagaactt ttgctgtatc tgatacagac tcttttttac
    13801 ccatatgaca atcatttgga cctagataag ttacatatgc tccttcatca tgagctgcta
    13861 cttcaaatga acatctagtt cttgttgatg ttttctcaaa taataaagct atattttttc
    13921 cttgtagcaa atcgcctttt atgccaactt ttttcttagt ttttaaataa cttgataaag
    13981 ttaataaata tctaatctct tcttttgtaa aatcctttaa tgtcaaaaaa ctcttacctt
    14041 ttaaacttat attattcata ttaattcgcc ccttcttata aacttcttaa attcaattaa
    14101 acttgttagc actttacttt tttatttact tctaattttt atctttaatt aactattttt
    14161 tgaatattta gttaatctct agtaatatat cttttaggat ttcaagagcc tcatctattt
    14221 ccttataact gattgttaat ggtggtaaaa atctcaatac attttttcca gcagtcaaaa
    14281 taagcaatcc tttttttcta gctttctctt ctataatact agattcaata ttagtttcaa
    14341 ttcctatcat tagaccaata ccttttgtct ttaatacaac tttattatta aaactttcaa
    14401 tagattttct tatataattt cctttttcta atacttcatt ataaaaacca tctttagata
    14461 tctcatctaa tacaactgaa gctgccacca tagctaataa attacctcca aaagttgtac
    14521 cttggtctcc ttttcctagt acttttgaaa gttttttatt taccaatact cctcctactg
    14581 gtattcctgc tcccaaccct tttgctacgg taactatatc tggttttaca tcaaaatatt
    14641 catagccaaa aagtttacca gttcttccta taccgcattg gacttcatca aaaatcacta
    14701 caatatcctt ttcttgacat atttttacta tttccaaaac ataatctttt tcaagaacat
    14761 taactccacc ttcgccttga atcgcttcaa gaattattgc acaaactgtg gaatcaagat
    14821 tattttttaa atcttcaata ttatttcttt ctatatattt aaaaccttcg ggaagaggat
    14881 aaaaatactt atggtatttg tccataccag ttgccattaa agacatcatt gttctaccat
    14941 gaaaagagtc ttttaaactt atgatagttc ctctaccatt accatatttg tcactactat
    15001 actttcttgc tattttgaat gctgtttcat ttgcttctgt tccagaatta caaaaatata
    15061 ctttagccat tccacttgaa ttaattattt tctctcctga atacaacact ggctcattaa
    15121 aatataaatt tgaagtatgt aataatttat ctacttgctc ttttagagca ttctttaaat
    15181 tactattact atacccaaga gaagatactc catatccact tgtaaaatct atgtatttat
    15241 tgccattagt atcaaaaaaa caacttcctt caccataatc tatgacaaaa ttgggttggt
    15301 tatatgtatc tataaaatat tcattccatt ttgaaattgt attgttgtta ttcattctta
    15361 ttctcccctc ttaaacttat aattttattc attttctttt cttattaatg tacctattcc
    15421 actatcagta aatagctctg ttattataga atgtggaact cttccatcca aaatatgtac
    15481 cctatttact ccgttattta aggcatctac gcatccctct attttaggta tcatacctcc
    15541 agtaattata ccttcttcaa ataatttatc aacatcctct aatataactt ctgttattaa
    15601 tgatttttct tcatctggct ctcttaacaa accagggaca tctgtaagaa gtatcaattt
    15661 atctgcattt aactctttag ctatagcaga agctgctgtg tcaccattta tattataagt
    15721 ctctccatca tctccaacac ctatagttgc aattactgaa atataacctg actttagaca
    15781 agattctata agctcaacat ttactttttt gatttctcct acaaatccaa gttcataatt
    15841 tttatatggg tcacataaaa tcatattatt atccattcca caaagcccta ctgcttttcc
    15901 accttttgta tgtattttat ttactaggtc tttattaacc tttccagcaa gtaccatttt
    15961 tacaatttcc atggtctctt catctgtata tctaagacca tttacaaatt tactttctat
    16021 atccactttt gctagcatct tattgatttc tgcccctcca ccatgtacta aaactatatt
    16081 gattccaaca taactcatta atacaaggtc ttccataaca gattctttta gaccatcttt
    16141 tttcattgca cttccaccat acttaactac aatagtcttt ccctgatgtt tttctatata
    16201 tggtaaagct tctataagtg tatttgcttt ttctatatta atcattaaag tatatcctcc
    16261 taatttctat aacttccatt tattcttaca taatcatagc ttaaatcaca tccccatgca
    16321 tttcctctgg cattgcccat atttaaatct actaatatac ttatttcgtc atcttcaagg
    16381 attttttttg ctttttcctc attaaaatct aatccatttc catttttaca tacttcaata
    16441 tatcctttca tactctcaaa aataatatca actttttcca tatctatttc ttcacctgca
    16501 taacctaaag cacacagtat tctaccccaa tttgcgtcag ctccaaatac agctgtcttt
    16561 actaatgaag aagatataac tgtttttgct aattttactg catgttcttc acttaatgct
    16621 ccatttatat aacattcaat tagttttgta catccttctc catcttttgc aacaagttta
    16681 gcaagttcta tacagacata tgtaagtgca ctcaaaaata cttgataatg ttcatctttt
    16741 ttgtttattc tatcattttt tgcttttcca tttgctagaa tcatcaccat atcattagta
    16801 cttgtatctc catctacact aactctatta aaacttttat caacagcaat tttcaaagct
    16861 tcttttagca aaactccatc aatatttgca tctgtagtta taaatcctaa cgttgttgcc
    16921 atattaggat gtatcatgcc agaacctttt gccatacctc caatagttat ttttttatct
    16981 ccatacatta ttgctactgc tatttctttt tttataatat cagttgtcat aatagcttct
    17041 cttgctccaa tatgaccttg tttacttaga ttattcacta attcatccat attttcttct
    17101 attaaattta tatttaatgg ctttcctatg acacctgtag atgcaaccaa aacatcattt
    17161 tctttaatat ttaatttatc actagtgaat ttagccattt tataagcatt tataagtcct
    17221 tcttttccat taaaggtatt agcattagca ctatttacta tgatagcttg tgcttttcca
    17281 tttttaatgt gttcctgtgt aacataaaca ggattgccct ttactttatt ttgagttact
    17341 actgctgctg catcacataa aacatctgaa taaaccaaag ctaaatctct tttttcttta
    17401 ttttttctca agccacagtg aatccctgaa gcaaaaaatc cttctgaaac tgtaactcca
    17461 ccctttataa tctccatatt tatcctccta attatatgag tttagtattt tttcattact
    17521 aaaatgatgg tgcaatattt tttaaacctg tattttcttc aataccaaat attatattca
    17581 tgttttgtat agcttgacct gctgctccct taatcatatt atctatagct gaacagatta
    17641 tcaatgtatc tccattttca tgtaatgata tgacacattt atttgagaac ctaacattct
    17701 ttaaagaagc taccttatct aatgggagaa cttctataaa ttctttaaat tcataataat
    17761 cagttaattt
//
LOCUS       DAEPOG010000074        17740 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEPOG010000074 DAEPOG010000000
VERSION     DAEPOG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.074"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(88..294)
                     /locus_tag="KR432_002050"
     CDS             complement(88..294)
                     /locus_tag="KR432_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640581.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            599..790
                     /locus_tag="KR432_002051"
     CDS             599..790
                     /locus_tag="KR432_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640582.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(879..1691)
                     /locus_tag="KR432_002052"
     CDS             complement(879..1691)
                     /locus_tag="KR432_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBH0640583.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            2113..3264
                     /locus_tag="KR432_002053"
     CDS             2113..3264
                     /locus_tag="KR432_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0640584.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3382..4020
                     /locus_tag="KR432_002054"
     CDS             3382..4020
                     /locus_tag="KR432_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0640585.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            4021..5043
                     /locus_tag="KR432_002055"
     CDS             4021..5043
                     /locus_tag="KR432_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH0640586.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            5033..5653
                     /locus_tag="KR432_002056"
     CDS             5033..5653
                     /locus_tag="KR432_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640587.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5777..6463)
                     /locus_tag="KR432_002057"
     CDS             complement(5777..6463)
                     /locus_tag="KR432_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBH0640588.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6477..7022)
                     /locus_tag="KR432_002058"
     CDS             complement(6477..7022)
                     /locus_tag="KR432_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0640589.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7250..8497
                     /locus_tag="KR432_002059"
     CDS             7250..8497
                     /locus_tag="KR432_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0640590.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8584..10578
                     /locus_tag="KR432_002060"
     CDS             8584..10578
                     /locus_tag="KR432_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0640591.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10578..11900
                     /locus_tag="KR432_002061"
     CDS             10578..11900
                     /locus_tag="KR432_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0640592.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            12179..13465
                     /locus_tag="KR432_002062"
     CDS             12179..13465
                     /locus_tag="KR432_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0640593.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13551..14858
                     /gene="grdB"
                     /locus_tag="KR432_002063"
     CDS             13551..14858
                     /gene="grdB"
                     /locus_tag="KR432_002063"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14598..14600,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0640594.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(15074..15298)
                     /locus_tag="KR432_002064"
     CDS             complement(15074..15298)
                     /locus_tag="KR432_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0640595.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(15395..16648)
                     /locus_tag="KR432_002065"
     CDS             complement(15395..16648)
                     /locus_tag="KR432_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH0640596.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            16908..>17740
                     /locus_tag="KR432_002066"
     CDS             16908..>17740
                     /locus_tag="KR432_002066"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH0640597.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     D"
BASE COUNT         7087 a         1895 c         2898 g         5860 t
ORIGIN      
        1 tattttatgt acaatcaatt taacaaagaa ttaactataa ttgtaaaaaa tatgtaaaaa
       61 aatagcatcc ttttagaatg atatttttta gttataaata tttttatatt caattggtgt
      121 aaggttatta aactttttaa aaattatact atagtaattt tgactattga atccaattag
      181 tacagtaacg tctaaaatag acaagtcttt ttttaataga tactttttgt ttttttcaac
      241 tctaacttta tttaaaaaat tagtaaaagt atatccactt tttttcttaa acaaagaaaa
      301 aaataacttt tattaagttg taattcatta catatattac ttactgttat atcttctgag
      361 tagtttttgt gaatataatc tatagttttc tttaagtttt ctttacataa aactaagagt
      421 tggtctatgt ttcattttat caaggcatat ttattaaaaa atcaatctct atttgttaaa
      481 ataatttatg aatgatgtta gaaactgtaa tatattggat ataatataat aaacagataa
      541 actctgtatt aagaggatat atagtttatc ttaattatag gggaagtgag gatatattat
      601 ggaaagatta atggaactca tagaaagaaa atttttaaag gatgtagaac ttttagaatt
      661 agaagacata gaaaatgtaa tagaatgcaa aatgacacag ccatataatg ctcattataa
      721 tgaatactta gtaaaagtaa aaaatggaga tgaagtagaa gaatattttg tgtatttaaa
      781 acatagataa acataaaaga ggtttatatt tatttgtatg attataaaat aaatacaagc
      841 ctcttttttt gataaatata aaattataaa atttttattt atttaatttt ttcaacaaca
      901 ttagtcaata ttgaatctaa atcatcacta atttctgtaa ctccattatg agtaatatct
      961 ataatttctt tagtcatagg aagttctcct aaaagctcta agtctaaatc atctaaaaac
     1021 ttttcagtag attctccttc aaataacttg atttttttag aacaatcagg acattggata
     1081 taactcatat tttcaataac tccaaggaca tttatgttca tttttttagc catattaaca
     1141 gcttttgata caatcataga taccaaatct tgaggtactg aaaccataac tattccactt
     1201 ataggaatag attgcataac cgttaaggca acatctccag taccaggagg catatctatt
     1261 aataaatagt ctaattcttc ccaaagtaca tcagtataaa attgctttac aactcctcca
     1321 agtaaaggtc ctctccaaac aacaggttca ttctcatcat ctatcataag gtttatagac
     1381 attactttta tgttatttga gttttctaca gggtaaatat aagagccatc agaataagct
     1441 tttacatttt ttacacccat aagtctagga atacttggtc cagttatatc tgaatctaat
     1501 ataccaacct tatatcccat tttgtttagc ttattagcaa gtaaagcagt tacagttgat
     1561 tttccaactc cacccttccc actcataaca ccaattattt ttccaaattt attgttaggg
     1621 ttattttcaa tagaacaatt tgattgacta ttacaatttc cctttgatgg acatgaatta
     1681 caatctgcca tatctattac ctccaaactt taatatttat tagattattt gtaacctaca
     1741 tatactattt tagtacaatt gaaccataaa gtcattattt aaaagcaact tagaagattt
     1801 tctattgatt tattcataga ctatagcaaa aagaatacaa taaaaaaatt ataacattct
     1861 tgaaaaagtg attatttcaa tttagttata gaacttagtc atataaatat atagaaaata
     1921 ttttaatata ttatacataa cgggttattt tataagttta actataaatt aggtttatat
     1981 tttataaaaa tttgttaaaa aaaacaatta aaaattgtaa agaaaaaaga tataattaga
     2041 actgtagtaa taaatgtttt attatagata atagagtttt aagtatctta aataatattt
     2101 ggaggaatat gaatggaatt aagaccaaga agtgcaaaat tagaagaatt tgataaagta
     2161 atagaattaa taaactatgt atttagaata tcaagaaatc ataagccaac tatgatggaa
     2221 gaatttcctc ttttactaag taaaaataat atagaaaata tgattataat atcagaagat
     2281 gataaagtgg tttctgatgt aaactatcta attcaagatg tatctattca aggaaataga
     2341 ttgaaggttg cagctatagg tggagtatgt actcatccag attatgaaaa gagaggctat
     2401 tcatctaaga tattggataa agtagaagaa aaaatgtttt atgatggtgt agatattgta
     2461 attatatcag gaacaagaag tctatacagt agaagaaatt gtagtttggt aaagagtttt
     2521 tataaatata caataaaacc tgaagatgta aaaatagcat atgaaatagt tgaatttgat
     2581 gaaactaatt ttgaaaaaga tgatgattta gataaaatgc ttgaactata taatcaaaat
     2641 agtactagat ttataagaac gagagatgag tttcaaaaac tattgcatgc agcaactatt
     2701 gcctggggac caattggtta caagaaggta tttataaagg aaaataataa tataatagga
     2761 tatttgataa taagaacaat aaaaaaagaa gattctactg taggagaagt tgctgaaata
     2821 gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg ttgcaaataa atttggatta
     2881 gaatatttaa attataaagt acatgtgaaa aatcttaaag accagttgaa atgtaatggg
     2941 acagaatctt tggactatca acaaggaact atgaaaataa taaattttac taagttgtgt
     3001 gatagtttaa gaagttattt tagtcaatat gtagattttg aattactaaa atacatggaa
     3061 tttaaacaag ttgaaaataa atatattatt aaatacaaag aagaagaact tgtaatagaa
     3121 aatttggata agcttaataa actgtttttt gaaaaaaatg aggaacagta taatgaattt
     3181 aaacatctaa aaaatatata tgaatttgct acaaaggcat ttcctgtaga ttttccatgg
     3241 actgcaaatt taaattatca atagttttag atgatattaa ataaaattga ttgataccta
     3301 gagttagtcc ttgttaaata agagaaatta ttggatattg aaaaagcaat tttgtaagta
     3361 attagaaagg atgagtatta tatggacata cttgatgata tagcagaatg tatattaaat
     3421 atgggtgtag ataatataga gaatttagta aaaattgcta tagataaaaa tattgatgtg
     3481 gaagatattt atgaatatgg tttaaacaaa ggtatgatag gagctttaga taagtttgag
     3541 aataaggaat actatctttc agaagttata gtatgcacag atgcattaaa taaaggtatt
     3601 aatcttttga agggaactgg aaaagtaaag aaaaaatcaa agggagtaat tctgatgtct
     3661 gtagtagaag gagatactca tgagatagga aaaaatattg taaaagtaat ggtagaagcc
     3721 actggatata aggttattga cttaggtgta aatagaaaaa gtgaagatat aatagaagaa
     3781 gctataaaaa acaatgtaga tataataggc ttatcttcaa tgatgacaac tacaatggaa
     3841 aatatgaaat cggttataga cgagcttaat atgattgaga tagataaaag accaaaagta
     3901 ataattggtg gaggacctgt aagtatggag tttgcagaag aaataggagc agatggatac
     3961 agttcaaatg ctccaaaagc agttaaatta ataaacaaac ttataggagg tgaagtttaa
     4021 atgttttcag atatgacttt acttcagtat ataaatagtg aagatagagg tttttttctg
     4081 cctgatatgg gtacaaatgg attgttctta ataaataaaa aggcatatga agtatatgat
     4141 agtgtagatt tgcaattaga attagcaaag actatggaca agtattttga atcagatttt
     4201 atatactcat tctgtgatgg gattacattt tgtgaaactc tgggtttaga gacattaaga
     4261 ccagattatg attttccaag tgtattgaca catactataa ctgacagaga taaattaaga
     4321 aaattagatg ttccagaccc ttatacgagt ggacgtatgc cactcaatat agagagctta
     4381 tcagttatat caaaaagtat taaaaaacca ctatatgaat cgatacaagg tccatttaca
     4441 cttgcaggtc aattagctgg agctactcaa cttttaagat gtataataac tgataaaaaa
     4501 tttgtagaag aattgttgga atttacaact gaattggtaa gaagatatgc tgtagctgca
     4561 aataaagcag gagcaaaata tatttcaata gcagagccaa cgtcagtaac cctaagtaaa
     4621 gatagatttg atgaatatat agtaaaaaat ttaaataaaa tctacaatga acttgattgt
     4681 tggaaaggta tgcatatatg tggagacacc agagaattat tagataatat gctaagttgt
     4741 aatatagatg cagtaagttt agaccaaatt ttagactatg aagaaattgc tccaatgatt
     4801 cctagagata tagtgttgat tggtaattta gacccaataa aacttcttgg acgttctaag
     4861 ccagataaga ttagaagaga gacactaaaa ttgttaaaga agatgagagg atatgataac
     4921 tttttatgtg attttggctg taactgctta aataccactc cagtagaaaa tttacaagca
     4981 gctataaaag caggaagaat taattataaa gaattagata ggattgatat tgatgaatta
     5041 taatagtaat aaaaataaat atcttttaaa ggatataaat ttagaggata tttttataga
     5101 taagaatatt gtgctaaagt ttttggggta tggaaataga aaagctccag atacaattag
     5161 tgaaatcgtt gaaagagaaa taagaaatat aaatgatatt ttagatatca agatatatat
     5221 aagtgaagtt gatattaata aaacacctaa agaatgtaaa aaagcatttg ctgtactata
     5281 tacaataggg gataaaattg atattaagat gaatgactat atggaaaatt gcaatatgat
     5341 ggcagggata gcattggata aaataggtat agtatgttta gattatatta atgagaaaat
     5401 aaaaatgtat ttaaaaggaa agtatgatag tcttaaaata tcgcatgaaa tatatccagg
     5461 agataaagat tttgaagtag agagacaaaa ggatatttat aactatgtaa aaaataaata
     5521 taatagtata gaaatagaaa tcaatgatta tcatcaacta tcccccataa aaagtgttgc
     5581 aatgcttatt ttaatgggag atgaagaaaa tttagaatca agatgtagca aatgtcctag
     5641 aaaatgcttt taactagttt tcaagactaa gatttgtttt gtgtatgaat tgcaataatt
     5701 tttttggtca ttttatgtat aaactaaaaa aattattgca aagtaatatt ctatttaatt
     5761 tgaacagact tacaaactat agtagtttct ttaattcaat aagtgtattt atttcataag
     5821 ttggtatatg attagtataa tttatactgt tattggaatt taaccaacaa gtatctattc
     5881 ctgaatttat tccacctaag acatcagagg ttaagctatt cccaatcatt aaggcagaag
     5941 atttatcatg acaattaaac tttttgagag cgtatttaaa tatttcagga tttggttttg
     6001 atattttcaa ctcttcagat acaaccattc catcaatata tccttttatt tttgaatttt
     6061 caagtctttt cttttgaact gcagaaattc catttgttat tataacaata gtatactttt
     6121 ttttaagata ttgtaatata tcgattgcac ctggaattaa aaatgtacat tcacctaaaa
     6181 aatttaagta cattttgcta agagtttcag aatcaacatc caaagatatt ttatttgcaa
     6241 aaagttcaaa tctaagtaat ttaagttcat tcaaagttat aatatttttt tcaaggtcga
     6301 gccaaagctt atcacttata tttctatatg actctatata attctcaaaa ttaaattcta
     6361 aatcaaactc agatagaagt tttttaaaag catgtgattc acttttctta aaatcaaata
     6421 aagtatcatc tgcatcaaaa aatataaatt tatatgtctt catggttttt actcctttac
     6481 aagatgactg caaaaattac tttactttca ctatttgagt cattttgcca tttatgcttt
     6541 gttcctaaag gaacttttac actatctcct ttggacaaag taaattcatc atcatccata
     6601 aaaagtttaa cttctccctc caaaacatat gcaatttcat ctccattatg agtaatccta
     6661 tcaatacaag aagaagtatt tggagctaaa tccataattg caaattctaa attaccacta
     6721 gagtctggaa ttaaaatctc atatataact tctttgcttc caggtaaaat aacttttttt
     6781 ctattatcat gtcttactat caagtttttt ttatctactt cagatacaaa aaatgtaaat
     6841 aaaggaacgt ttagagaaga ggctatagat tttaatgaat ttacagatgg attagcaaga
     6901 cctcgttcta tttggctcaa taatgatggt gtaacatcag ctagttttgc taaatctcga
     6961 atacttaagt tttgtttttt tcgaacttca gctatttttt cccctaaatt taaatcattc
     7021 atataaatat cctctctttt taatttaaaa aatattaact taaaataatc aagtaaaatg
     7081 attatgtagt atgaaattat tataacatat agaattaggt tttgaaaaaa ctatgttcaa
     7141 tataattaaa taaattaaaa tataattgac aaagtggata cagaatactt aaaatatatt
     7201 taaataaaat taagcaaaag atagttaatt tatgatagga ggggacatta tgcaaataac
     7261 gacttttgga gctatatttg gtttattaat agcaattatt ttaattataa aaaaatttca
     7321 agctgtatat agtttaatgt taggagcatt tattggagga ttagttggag gagcaaatat
     7381 aacacaaact gtagatttta tggcaaatgg agctatgaat atatcaccat caattcttag
     7441 agctttggca tcaggtgtat tagctggaag tttaataaaa acaggagcag tagataaaat
     7501 atcagaacag attgtaaaaa tatttggtga aaagagggct ttattttcga ttgccatttc
     7561 aactatggta cttgctggag taggcgtaaa tttggatgta tctataataa ctgtagcacc
     7621 tataggatta tatataggta gaaaattaaa ttattctaaa ttgtcaatac tattagctat
     7681 gttgggaggg ggtaaagcag gtaatataat atcaccaaat ccaaatacta tagcagtagc
     7741 agataatttt tctgtcaatc tctctagtgt tatgatggca aatataattc cagcaattat
     7801 tggagttgtt attacagtta ttttagcaag tatattaata aataaaggaa ataaagtaca
     7861 atcatatgaa attttagagc aaagagaaga tttaccaagt ttatttaaat cattatgtgg
     7921 tccaattata gctatatttc tattgttttt aggaaatgta tctcctatag ttatagaccc
     7981 aatgatagcc cttccaatag gaggaatcgt aactctaatt gtcacaggaa acttgaacaa
     8041 ttcaagagaa tatttagctt ttggattatc taagatgcaa ggggtatgca tattattact
     8101 tggaacaggg actatcgcag gaattataca gatgtctgaa ttacaacaga gtacaatagg
     8161 tgctttacaa tttttaaata tgccacaatt tttattggca ccagtttcgg gtatacttat
     8221 gtcattagca acagcatcat caacagctgg tgctactata gcatcatcta cttttcatga
     8281 tgctataata aatggtgggc tatcaccgat ttcaggtgca tcaatagtaa atgctgggtc
     8341 tagtgtattc gagcaattac cacatggctc actatttcat actagttcag gtagtataaa
     8401 catggatata ggtgaaagat ttaagttaat tccatatgaa gcattgatag gaatagtaat
     8461 gactataatt tcaacatcta ttcaattagt tttataataa atataaatat ttaatgaaaa
     8521 aagtaggatt ttgttattaa atattgaatt tattatagta aaaaagaaga ttggagtttt
     8581 aaagtgaata aaaaaaagat tgttattata gggattattt attcattttt agtagtattt
     8641 tcacttacaa atatgtatgt aaatatggag tataatttaa atgtatttga atatattaag
     8701 aagtcacttc catttacaga agaagaaaaa aagtggttag aaaaacacaa gaatttgatt
     8761 tacagctctg accaaagttc acctcctttg agatataagg gtaaggaaga tggacagtat
     8821 aagggaatta ttgttgattt aataaattct ctgtcaattc aaattggtag agatttttat
     8881 tttaaaccaa ataattggtg gaaagaatct ttcgtaaatt caatagatga tagtataaag
     8941 ttttttgact tgattccatc aaaagaaagg gcaaataaat ttatatttac tgacccaata
     9001 tacactttga gtgcaaatat attgaaagat aaaaagtccc aagatataaa tagttacatg
     9061 gatttaaagg ggaaaacagt agcaattcca gaaggtgatt attctataaa ttttttaaag
     9121 caaaaaattc aagatataaa cattttattg actccagata taaagactgg agtcaaccat
     9181 ttaatgtctg gaaaagtaga cgcagttgtt ggtgatgaac cagtattgcg atactatata
     9241 aataattatg gactttcaaa taaatacagt gttttaagta atcctatata tactaaaaaa
     9301 gcagttcttg ctgtacctaa acaatatgaa gagctggtta gtattttaaa taaaggcatt
     9361 tttaaacttc agaaaaatgg agtgtataaa gatttaaaga aaaaatggta ttctacgtat
     9421 aatgaagttg atgatatatt gtatgagcga ggaattgtgc ctagtattta tctttttata
     9481 ggtataattt taatatcaat ttatgttttt tatagctata catatttgtt aaagatagaa
     9541 atcaaaagga aaacagaaca ggtaatagaa aataaaaaaa ctttagaggc aacttttaat
     9601 agtattacag atataataat gcttgtagat gaaaataaca atatagtaga atcgaacaaa
     9661 gtactatatg actttatggg tgaaatgagt tataagatag cagatttgat tagcatgata
     9721 aaaggtgtta ttaaaaatac cttctctgaa aatactaata aaactagtga aatagaaata
     9781 tacaataaaa ttttaaagat aaacactttt cctgtagagt ataaaaaaaa taatactgag
     9841 tatatagtag ttcttattaa agatataact aatgaaaaaa tagtagaggc gaaattactt
     9901 agagaaaaca aaatgatatc tataggacaa ttagcttctg gggtagcaca tgagataaga
     9961 aatcctttgg gtataataag aaataattgc tatctattaa aagataacgt tacaatggaa
    10021 gaagtgaatg attgtgttaa atcaatagaa agtaatgtag atagggcaag taatataata
    10081 accaacttgt taaatttcgc aagaatatca gatgataatt tagaacatat aaatataaga
    10141 aattttattg aaaatatagt gaaacttcaa tataaaatgt tacagttaaa gaatgttgaa
    10201 ataaaaattg actgtgaaca taatttagta tgctatataa atggagaatc gttaaaacat
    10261 gtatttataa atttaatttc taattcaata gatgcaatac atcaagatgg aaaaattatt
    10321 atatactgtt atgaaaaaaa tcattgttta tttatagatt ttaaagataa tggtgaagga
    10381 ataaaagaag atgccctaaa ggacatattt aatcccttct atacaacaaa accaattggt
    10441 gagggaactg gtttaggttt atatattact tataatgaga ttaaaaagaa taatggagat
    10501 ataagtgttg aaagtaaatt gggggttgga acttgttttc atataaaaat tccattaaat
    10561 aaagaggtga ctatataatg aaaatactat tagttgatga tgagctagaa tatggagtgg
    10621 taatgaaaaa aattctacaa aagaagggct atcttgtaga tatagcacta agtggtgaag
    10681 aagctataaa tataattaaa aaagataaaa attatgattt ggttttatct gatgttatga
    10741 tgaaaaatat ggatggagta cagctattag atagaattaa atctattaat agagatatag
    10801 aggttatatt agttacagga tatggaagta ttgaaaatgc agttgatgct atgaaaagag
    10861 gagctttatc ttattttata aaaagtaatc ccattgaaaa tttattagaa gaagttgaaa
    10921 aggtaaaggc aagtaaaata tctgtttctg tacaaaaaaa taattttgaa tttactttgg
    10981 aatctaaaaa tagagatttt aataatatca taaaaatagc aaaaaaagct gcttgtaaag
    11041 atgttaatat attaatcttg ggggaatctg gtgtaggaaa agatatatta gctagatata
    11101 tacatagtat aagtccaaga aagaatgaga tatttgtacc tgtaaattgt tgttcttttt
    11161 cagaaaatct attggagtct gagttgtttg gtcatgaaaa aggttctttt acaggagcag
    11221 tagatagtag aaaaggtaga tttgaacttt cgaataaagg aactctattt ttggatgaaa
    11281 taggagatat acctctaaat gttcaggtaa aacttttgag gacacttgag gacaaatcta
    11341 tagagaggat aggaagtaat aaatctatta aagttgactt taggttaatt tgtgctatga
    11401 ataaagaacc aaaagtggaa atatcaaatg gaaatataag agaagatttt ttttatagaa
    11461 taagtacaat aacaataact ataccaccac taagaaagag aagagaagac ttaactactc
    11521 ttatagaatt tttcttaaat aagtaccaaa ttgaacatga taaaaagata cactcgattg
    11581 ataaggaagt aaaggatttt ttattaaatt acaattatcc aggaaatata agagaattaa
    11641 aaaatataat aaatagactt gtagttttaa gtgaagaagg caatttatca aaagacaatt
    11701 taaatttaat atcaaataat gtatacatag atgataaaat aagtattaga cctttaagag
    11761 aaataagaaa agagtttgaa tgtgagtata ttgaaaaagt tttatctttg tgtggaaata
    11821 atatttcaaa tacagctaaa aaattggaga ttagtagaag acagttaacc aataaaatat
    11881 cagagtataa tattaagtga gaaaaaaact tctcattaag tgagaaaaaa atttctcaaa
    11941 ctgtattaaa agataaagag aaaaaatctg tttatctttt tttattataa ttttaaaaat
    12001 taatatttac aataattatt ggtaaattca atactggtgt aaattaaaaa tgaaaatttc
    12061 tatgacatta atacatagaa tatatatttt aaataattgg catagaaaat gctttatata
    12121 ttaataaacg taataaatat ttttaattgc gattaaatat attaaggggg attttcatat
    12181 gaagctagag ctagggaata tatttataaa aaatgttgaa tttggagaaa agacagaagt
    12241 aaaagatgga gtactatata ttagtagcga agaaatagaa aagatagtat tagaagatga
    12301 aaggattgta agtgtaaatg ttgaacttgc aagacctggc gaatcaataa gaatagctcc
    12361 agttaaagat gtaatagagc ctagagttaa aattggagat gaaagcaaaa tatttcctgg
    12421 aattataaat aaagtgaaga ctgtaggaag tggaagaacc catgtacttt taggagcttg
    12481 tgttgtaact tgtggaaata tagttggatt tcaagaaggc gtaattgata tgagtggtcc
    12541 tactgctaaa tatacacctt tttcaaaaac aaataatata tgtattgtta taaaagcaaa
    12601 ggatggtata gatactcatg attatgaaga agcagctaga attgcaggat taaaaatagg
    12661 tgcatatgta ggtgaggcag gaagagaagt tgagccagat gaagtcattg tatatgagac
    12721 aaagccatta ttaaaacaag taaaagaata tccagacctg ccaacagtag catatgttca
    12781 catgttacaa tcacaaggac ttttacatga cacttattac tatggggtgg atgctaaaca
    12841 gatagtgcct acatttatgt atccaactga aataatggat ggagctataa tatctggaaa
    12901 ctgtgtagca ccatgtgata aggttacaac attccatcat ttaaataatc cagttataca
    12961 tgacttatat aaaagacatg gaaaagattt aaactttata ggagttattt tgactaatga
    13021 gaacgtattc ttagtcgata aagagagaag ctctgatatg gtagctaaat taatagaatt
    13081 tttaggagtt gatggagttc tagtgacaga agaaggttat gggaatccag atactgacct
    13141 aatgatgaat tgtagaaaat gtagtgaagt tggagcaaat gtagttttga taacggatga
    13201 atttcctgga aaagatggca agagtcaatc tatagcagac gctactaagg aagcagatgc
    13261 agttgtttct tgtggtcaag gaaatctagt ggttcatttc ccagcaatgg aaaaaattat
    13321 aggaacttta gattatgttg aaatgatgat aggtgggtat aaaggatgct taaatgaaga
    13381 cggaagcatg gatgcagagc tacaaattat aatagcatca actatagcaa atggctataa
    13441 tcatttgaca gcaagatatt attaaataag tatttgaata taaagttctg ttgatgtaat
    13501 cgttggtcta attgatgtaa taactataaa gtgatttgga ggattaagag atgagtaaaa
    13561 tcagagtggt tcattatata aacaatttct ttgctggtat aggtggagag gaaaaagcag
    13621 atattccgcc agaaaaaagg gctggtgtag taggacctgg aatagcattt cagagtcaat
    13681 ttaatgatga tgcagagata atctcaactg taatttgtgg agatacgtat tttggtgaaa
    13741 atattgagag tgcaactcaa gaacttttaa aaatgataaa agaagaaagc cccgatttat
    13801 ttattgcagg cccagcattt aatgcaggta gatatggagt agcttgtggg actatttgta
    13861 aggcagttga agaagagtta aatattcctg tagttacagg aatgtataaa gaaaatccag
    13921 gtgtagatat gtttaaatta gatttgcata tagtgtctac aggaaattca gcagcaagtc
    13981 ttagaaaggt agttccaata atgacgaaac taggtcttaa attggttaga ggtgaggaaa
    14041 taggacctcc tgaagaggaa ggatatataa tgagaggaat aagaaaaaat ttcttccatg
    14101 aacttagagg gtctgaaaga gcaattgata tgttggttaa aaagatgaaa ggggaaagct
    14161 ttgaaactga gtatccaatg cctgaatttg atagagttac acctatgaaa cctgttaaag
    14221 atttatctaa gataaaactt gccctagtaa cttctggagg gatagttcca attgataatc
    14281 cagacaaaat tgaatcttca agtgctacta aatatggaat ttacgattta acagatatgg
    14341 attctatgtc aaataaagaa tttacaacta tacatggggg gtatgacaga gcatatgtcc
    14401 ttgaaaatcc aaatttagtt gtaccattag atgtagttag agagcttgaa aaagaaggtg
    14461 taattggaga attagtagat tattttataa ctactacagg aacaggaacg agtgttggca
    14521 attctaagag atttggagaa gagttttcta agaaattgtt agaagatgat gtagatgctg
    14581 taattttaac ttctacatga ggtacttgta ctcgttgtgg agcaacgatg gtaaaagaga
    14641 ttgagagagc tggtatagca gttgttcata tatgtacagt agtaccaata tcacttacaa
    14701 taggagctaa tagaattgtt ccagcagtag ggataccata tccacttgga aatcctatgt
    14761 taggagaaga ggaaagtaaa aaaattagaa agaatatagt tttaaaagct ttaaatgcac
    14821 taacagagga tatagaagaa caaacagtat ttgaataata aaaatataat atagaaataa
    14881 aaaaagatat cataaaatag tttttgaaaa ttattttatg atatcttttt gctatggatg
    14941 gaatgaagaa agcaattaag taataaaaat atttatagta aaacagccaa ttaaagtata
    15001 aagaatgaaa aaaataaata gaagattata cataaacaaa aaaagagatt ttaaaatctc
    15061 tttttattat atattaatta ttttttaaat ttatattttt tgcatcagat tttctaatag
    15121 ctaagtcaaa ccctctaaat ctaatcacta tagggtcatt aagaggagct atctttttca
    15181 cttcaacttc agtgccatct attagtccta aggctagaag tctcttcata agtttttcat
    15241 ttccagctat attgtctata attccctttt gtccaagttt taagttgtaa acagtcatta
    15301 ttaacacctc ctaatgaaaa tcattatcaa actttatatt tataatatat aacaaaaaga
    15361 gtaaattgtc aatatataac aaaaaataaa catactaagt tagtatgttt ataaaaaata
    15421 gtatacatgg gatactaata gcatctagca aaaaaccact tactaatggt acaaccaaaa
    15481 aggcttttgg agaatcacca tatttttgtg tcaaagaact catatttgcc aaagcgttcg
    15541 gagtagctcc tattccatgt ccaatcaaac cacttattat gatggcagaa tcaaaatctt
    15601 ttccaagtac tctaaagcaa ataaatattg aaaataggat tataaagatt acttgaaaag
    15661 ttactattaa gagaatagga ggtaataatg tagataattc aaataaatct atattcataa
    15721 gtgataaagt taaaaaaatt cccaatgaaa gttcttttaa aaaatctata agtttaaaat
    15781 ttaattctaa tatatttatc ttatcattta aatttctaaa aataacagct gaaaacatac
    15841 aagttacaac agagggtaat agtatattaa acgttatata gaagcatcta cttataattt
    15901 ctcctgtatt tatacaaata aatatgagaa gaacctgttc caaaaataca gatggtgtta
    15961 ttgtttcgga gaagttatta ttttttatcc tattcttatt tctaaaaaat ttacttccaa
    16021 atctctttag atttctatta tatctaggaa tcgacaagcc tatatttgat gatggtttta
    16081 atttatattt atttattaga aatttagcta caggacatcc caatatacca cccataataa
    16141 gtcctattgt tgcagcagat aatccaaccc caactgctcc ttctataccg agattttcta
    16201 ttgtaagtcc aaatgcagca gaataaccat gaccaccttc catagataca tttccacaca
    16261 taagccctaa aagaggttca agttttataa tctttgatat tgtaatagct aatatatttt
    16321 gacaaaatgc caatactcca cacaaaatcc aatatctaaa taatacacta ccacctttct
    16381 ttataagatt tatgcttatt tcaagtccta tagttgtaaa aaagaaggtt ataaaatttg
    16441 gcattaaatt tccacttata gatatatctg ctatattaaa atatttaaaa aataaattta
    16501 gaaaacaaaa taatagacct cctattactg gtgcaggtat acagaaatta tttaaaaagt
    16561 taactttatt cttcaataaa ttaccaatta aaaagaagaa tatagatagt gtcaaggttt
    16621 gcactatatc aaaacttaag gtaatcaaaa taagtcctcc tttgtaattc aaagttttaa
    16681 ttatataact taaattctta tgtattgaaa ttttattatc aaatgtatat taataaatgt
    16741 atattaaaaa gaatcagtat atataattag atagtcataa attaaattaa aatattttaa
    16801 tgagatgtga cttttttaga atgaaaaacc gtagtatatt gatataatag aaataagact
    16861 taaaaatctg ttgatagtat tatttataag tacagtagga ggataaaatg tttgaaaatt
    16921 tacctaagat agatttacat tgccacttag atggaagtgt tagagtagaa accatgcttg
    16981 atatagctat taaagagaaa atagatttac cttcaaataa tatggatgaa ataaaaaaat
    17041 tagcaaaggt ttcttttaat tgtacttcac tagatgaata tttagaaaaa tttgatttac
    17101 ccttaaaagt aatgcaatct aaggaaaatt taaaaagaat tacttttgaa cttttggaag
    17161 atgcaagtag agaaaatgta aaatatattg aaattagatt tgcaccatta cttcacaccc
    17221 aaaaaggtat gagtgttaaa aatataattg agggtataat tgaaggaatt agagaagctg
    17281 aaagtattta tgatataaaa ggcaatttaa tattaggttg tatgagaaca atgacctcaa
    17341 aagaggcatt attagtaatt gaagaaggga aatcatttgt aaataaaggt gttgtagcag
    17401 tagacttgtg tggtccagag aaagagggat tttgtaagga atataaagat gtttttaaat
    17461 tagcaagaga atatggatat aaagttacaa tacatgcagg agaagctgca agtggagaaa
    17521 atgtattgga tgctataaat attttaaagg cggataggat tggtcatggg gtaaaaatta
    17581 aagaccataa aaaggcgtat aatttagtta aagataaaaa aatattgctt gaattatgtc
    17641 caactagtaa tgtacaaact aagacagtag attcttatga ggtacatcca ttttatactt
    17701 tttataaaga taatttacat gtaagtataa atactgataa
//
LOCUS       DAEPOG010000075        17625 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEPOG010000075 DAEPOG010000000
VERSION     DAEPOG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.075"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..602)
                     /gene="gmk"
                     /locus_tag="KR432_002067"
     CDS             complement(<1..602)
                     /gene="gmk"
                     /locus_tag="KR432_002067"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH0640598.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEK"
     gene            complement(632..1513)
                     /locus_tag="KR432_002068"
     CDS             complement(632..1513)
                     /locus_tag="KR432_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH0640599.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(1517..2347)
                     /locus_tag="KR432_002069"
     CDS             complement(1517..2347)
                     /locus_tag="KR432_002069"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH0640600.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(2634..3668)
                     /gene="ltaE"
                     /locus_tag="KR432_002070"
     CDS             complement(2634..3668)
                     /gene="ltaE"
                     /locus_tag="KR432_002070"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH0640601.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            3964..5739
                     /locus_tag="KR432_002071"
     CDS             3964..5739
                     /locus_tag="KR432_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH0640602.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(5954..7606)
                     /locus_tag="KR432_002072"
     CDS             complement(5954..7606)
                     /locus_tag="KR432_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0640603.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(7905..9116)
                     /locus_tag="KR432_002073"
     CDS             complement(7905..9116)
                     /locus_tag="KR432_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH0640604.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(9400..9933)
                     /gene="pyrR"
                     /locus_tag="KR432_002074"
     CDS             complement(9400..9933)
                     /gene="pyrR"
                     /locus_tag="KR432_002074"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBH0640605.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(10292..11239)
                     /locus_tag="KR432_002075"
     CDS             complement(10292..11239)
                     /locus_tag="KR432_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0640606.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(11208..11654)
                     /gene="lspA"
                     /locus_tag="KR432_002076"
     CDS             complement(11208..11654)
                     /gene="lspA"
                     /locus_tag="KR432_002076"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH0640607.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(11964..12743)
                     /locus_tag="KR432_002077"
     CDS             complement(11964..12743)
                     /locus_tag="KR432_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0640608.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(12793..13275)
                     /locus_tag="KR432_002078"
     CDS             complement(12793..13275)
                     /locus_tag="KR432_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0640609.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(13459..14880)
                     /locus_tag="KR432_002079"
     CDS             complement(13459..14880)
                     /locus_tag="KR432_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0640610.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(15026..15796)
                     /locus_tag="KR432_002080"
     CDS             complement(15026..15796)
                     /locus_tag="KR432_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640611.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(15801..17003)
                     /locus_tag="KR432_002081"
     CDS             complement(15801..17003)
                     /locus_tag="KR432_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0640612.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         5883 a         3103 c         1791 g         6848 t
ORIGIN      
        1 aatttctcta ttataatatt tttatatctg ctaactttat tcttttcaga tgatattata
       61 ccttctattt ctttagcaga tgtttttaca tcttcattaa atataaaata atcataatcc
      121 ttaatcattt taatttcttc atatgcacag ctaaatctct tctctatttc agcttctgtt
      181 tctgtacctc ttccaacaat tctatttttt aattcttcaa gtgatggtgg tagtacaaat
      241 ataaatactc cctctgggca aacttctttt atttgttttg ctccttgcat ttcaatctcc
      301 agtaatacat cttgtccttt ttctaaacat tccattattg ccgcttttgg agttccataa
      361 aaattatcat aaatttgagc atactccaaa aactcacctt tttctatcat ctcttcaaac
      421 ttttccttac ttatgaagaa ataatttact ccatcaactt ctccagttct gggttttctt
      481 gtagtagctg atacagaaag ttttattgta tcattttctt taagtagttc tttgcaaata
      541 gtaccttttc ctgcacctga tggtcctgat acaactagca ataatccttt tttcacaaac
      601 atttaagcat tccccttcta atattgtatt cttactctat attttgtatt tgttctctta
      661 ttttttccaa ttcgcttttt acttctacta ctaaattagt tatatttata tcagatgact
      721 tcgaacctat agtgttagtt tctctattca tctcttgtat taagaaatct atttttctac
      781 ctatagagtc gtctttaaat atagtatttt taagttgccc tatatgactt ttaaatctaa
      841 ctatttcttc tgttatactg cttttatctg catatattgc aacttcttgt gctaatctat
      901 tctcatctat tatacttggg tcatccagta attcacttat tctcaaattc aatttttctc
      961 tataatcttc tacaacacta cttgaatatt tttcaatctc ttctatatga ttttttaata
     1021 aatcacatct catggcaata tcttcagcta attttttacc ttcttcactt ctcatttctc
     1081 ttaatttgat taatgcatct tcaactgctt gattcaacat tgaccacaat aaatcttcat
     1141 cttcttcctt ttcttctatt ttcacaatat caggaaactt tgcaacattc ataacgctaa
     1201 tatcatctat aatatcaaat ttatttttta tttcttttag tatgtctata tactgagatg
     1261 ctaatccctc atcaaaattt aagtttacat cttctttgcc taataagtca aactttatgt
     1321 ataaatctac tcttcctctt tttacataat tttttactaa atttctaact ttatcctcta
     1381 aaaatgaaat ttttcttgga agacgtatgt ttatatctga atacttatga tttatcgttt
     1441 tacactctac taaaaagtaa taattatcat ccttgtattc tcctcttcca aaaccagtca
     1501 tacttatagc cattaattaa acctccaaaa caccttcaca tatttttact gctggacctg
     1561 tcatataaga gaaatctcct acatctattt taactgttcc accttgagac gttacattta
     1621 cactactttc taccatatta agtaaattgc acactattgc tgatgcagtc ataccagtac
     1681 cacaacctaa agtatatcca caacctcttt cccaagtata aacatgtata ttatacttat
     1741 cttctacctt tacaaaattt acattcgttt tttcaggaaa tattttatta ttttcaatct
     1801 cttttccata tttacaaaca tcatctacat ctaattcatc tactataact actgtatgag
     1861 gaacccccat caacatagaa gtaactttaa gctccctatc taaaaccttt atactttctt
     1921 gcacaaaacg ttctttattt gtaagtacag gaacatcctt agctataaaa cttccttttc
     1981 ccatattgac tcttatagag tttatttctc tattggtaag atttattttt atatctttta
     2041 taccatctaa tgtatctact gtaaaacttt cttttttaac tatacaatta tcataaacaa
     2101 atttacaaaa acatctaagg ccatttccac acatagctgc tcttgaacca tcagaattat
     2161 aatataccat ttctacatct gcaacttttg aatctttcac aactaacaat ccatccgctc
     2221 ctacagaaaa tcttctatgg catacttttt ttgctaaagc agtataatca gatggattta
     2281 tctggtcgaa tcttccatct attgctataa aatcatttcc tattccatgt agtttccaaa
     2341 atttcataag tatcgctcct tcgtaaatta acgcttaaac tattataaca taaaaaaatt
     2401 cccataaact tataatttat gggaaaagtt ttgtaatgac aactataatt tgaggatgtg
     2461 agggagagtt gtcattacaa ttagggtaat tatattatgt ttttaagcta gttctttcta
     2521 tgtttatata ttaatatttc tatatgaatt ttatgtatgt tttatttaaa ctgtctatga
     2581 attttttaat taaacatctg gtaaccagct ataatccttt tagtagatat ctattaacat
     2641 acacctatta attctttaag tgtttttaaa acatagtcta aatcttcctt agtaatatct
     2701 acatgagtta caaatctata ttcaccattt tccattttat taatttttat atttctttca
     2761 aaaaattctt ttattaaaac atcctctttt ataatatctt cactcatctt aaaaaatacc
     2821 atacttatat ctggattaac attgtttact tcaatgccat caattttatt taattcacaa
     2881 gctaaatatt ttgcattttc atggtcttca ttcaatctta aagtcatttt ttcaatggca
     2941 attataccag ctgctgcaac tatacctact tgtctcattc ccccacccat aactttttgc
     3001 tttcttctag cttttgctat aaattcttta cttccagcaa gtatagaacc cattggtgca
     3061 gctaaacctt ttgacacaca gaacataaca ctatcacaac actttgctat ttctttagct
     3121 tctacaccca aagttaatgc tgcgttaaat aatcttgctc catctaaatg tactggtaag
     3181 ttgtgtttct cagctatttt tttaatttct cttaaattat caagactcac aacagttcca
     3241 cctgaatatg cattttctac acatataagc cctgtctgtg gttcatgtat gtcatctctt
     3301 ctaattgcct tttcaacaag ttcaggattt agagctccac tttcgtcttc atctatagtt
     3361 ctaagctgaa ctccagctat tacagcaggt gccccaacct catgagttac aatgtgataa
     3421 cctttaccaa taattatttc ttgccctctt aggcaatgag taaatagagc taattgattc
     3481 ccaaaagttc cacttggaag gaaaatagca tcttcttttc caactttagc tgctgctaat
     3541 tcttcaagtt tatttacagt agggtcatct ctaaagattg aatctccaac aggagccttt
     3601 gccatagctt ctctcatttc gtcagtaggc atagtaacag tatcacttct taaatcgata
     3661 aatctcattt tttcaacctt ctttcatatt tatatactat agaaccttaa aattgatttt
     3721 ttctgtaaag ttcatatact ttcatagctt atactctaca caataattat actatattta
     3781 ctttatattt aatctacttt aaattattaa ttttaaaaat aaagtttcct ctctaataag
     3841 tataaataat tatacaattt aaatattaat ctctatcgtt taaattcccc ctagtcacaa
     3901 agcgtatatt atgtgtataa ttaatttact gactcatttt ggtcaaagaa aggggtgcta
     3961 taaatggcat tagatggatt agttatacat tcaatagtcg atgaattatc atcaaaactt
     4021 acaggtggaa aaatagataa gatacatcaa cctgaagatg atgaggtaat ttttaatata
     4081 agaaataata aagaaaattt tagattggtt ttaagtgcaa gtgcctcaaa tcctagagtt
     4141 tatcttacaa gtaattatca aaaagaaaac cctctaaaag cgcctatgtt ttgtatgtta
     4201 cttagaaaat acatacaagg tggaaatata gtagaaattt ctcaaatagg ttttgaaaga
     4261 ataataaaaa taagtgttga atctttagat gaattaaaag aaaagacagt taaaaatatt
     4321 atgattgaaa ttatgggaag acatagcaac ataataataa ctcatggtga agaaaataaa
     4381 attatagatt ctataaaaag agtccctttt agcataagta gagttcgtca agttttacct
     4441 ggtcatgatt attcattacc accagaacaa aacaaactca accctcttga tgatatatct
     4501 aaggatttgt ttattaaaaa cctagaagaa ttggaaggac ctatctttaa gtctatatat
     4561 tctaaatttc taggtataag ccctattata gctaaagaaa tttgctacag agcaggtgta
     4621 aatcaaaatg ctatcataaa ggatatatct gatgaacaat ttgattcttt acacaaagta
     4681 ttttgtaatt tattcaatga tataaattct aacaaatact ccccttgcat tattatagat
     4741 aaaaaggtgg ataaggtagt tgatttcagt tgtataaacc ttacattatt tagcgattta
     4801 tcatatataa ataaggatag tatgtctcgt attctagaag atttctatag aactaaagat
     4861 attaaagaca gaattaatca acgttcatct gatttaaaga agagtatatc tgttaagctt
     4921 gatagacttt ataataaact taaaaaacaa gaagaagaat tatcagaatc cgaaaatgca
     4981 gacatttata agataaaagg agaattgatt acttcatata tttatatggt agaaaaaggt
     5041 atggaaagta ttgaaattgc taacttctat gatgaaaatt gtaatgatgt aacaatagaa
     5101 ttaaataaaa acttgacacc atctgaaaat gctcaaaaat actttaaaaa gtataacaaa
     5161 atgaaacatg ctaaagtcga gatatctcat caaatttcct taaataagga agaaatagat
     5221 tacttagaaa acatagtttt aagtattgaa aactgcgaaa atttagctga attacaagat
     5281 ataaaagaag aacttgctaa ggttggatat atcaagactc aaaagaaaaa cagtaaaaag
     5341 gatactatcc catctacaaa acctcatgaa tttgtgtctt ctgatggatt taaaatattg
     5401 gttggtaaaa acaataagca aaatgactat cttacactaa gacttgctga taatgatgac
     5461 ttatggatgc atacaaagaa tattcctggt tctcatgtaa taataaagtg tgctggtaag
     5521 gaagtccctg ataatactgt atttgaagga gctatgctag cagcattctt tagtaagtca
     5581 aaactttctt ctcaggtgcc tgtagactat actaagagaa aaaatgtgaa aaaacctagt
     5641 ggttctaaac caggtatggt tatttatgaa actaacagta ctatttatgt tactcctgaa
     5701 gaagaaacag tagccaagct taaggttaaa tctgaataaa ttctgcacta aatacaaaat
     5761 aaatgttaga caagcacaca ttatcattct aaataaaaat agtggccttc ttataaagta
     5821 caataataag gccactttct aattagctta cttttaattt tctaacaaaa tacttttctg
     5881 ttttaataac tatacttata ttcccaacaa atattttcag tcttcttata tagatgtaat
     5941 taaaaagtta acactaaaag ctatttttta atttagtgat ttgttttagt aattcattat
     6001 attctgattc atacaattct ttttttagat tatctttttc tatagataac atagatataa
     6061 cagttgtaag tctattttct aatactagaa gattctcttt tttgatttgc ttgtctctac
     6121 taggagtttt tttatttctg ctaattatat attcgtcata attacaatca aactccctta
     6181 ttttagcatc ttttatctct ataatatagt tacaaatatg tgatacaaat actctatcat
     6241 gagacactat aagcatagtt ttatcagtat ttatcagtgc cttttccagt gcttccatag
     6301 attttatatc taagtaattt gttggttcat caaatactaa aaaattatta tcttccaata
     6361 ttattttaca tagtgctatt tttacctttt ctccaccact aagtactttt acttttttgt
     6421 agacatcatc acctttaaac ccaaataggc ttaagtttat ccttataaaa gattcatcaa
     6481 atgaactatt aactttagta ttgtatagaa cactcttttc ctcatctaat aaactttgat
     6541 tttggtcaaa acaacctact tttaccttgt tatttatttt tatattgtca tttttatcag
     6601 ccaatatttc ttttatcaga gtactttttc cacacccatt atcacctaat aatgctatct
     6661 ttttacctct ttttatctta aaggaaacat tatctaacaa gagcttattt tctaacttta
     6721 gagtcatatc ttttacctct aatagatttt tgctaattat ctccatacca tcttgaatct
     6781 taatgttcat ttctttagaa actttaggtt tattctttac ctcaagttta tcaattctgc
     6841 tttttatagc cttcacattt gagtccaact ttttcttatt agtctgacca cccattttgt
     6901 gcaacctagc ttctgaatta cccattcttt ttggtgtctt tcttatgcca tcacttaaag
     6961 cttttttata taaaagagct ttttcaagcc tttttttctc tttaacatat tcttgatact
     7021 caaaatcagc tctttttact tcttcatctt tttgcattaa ataatcagtg taattgcctt
     7081 tgtaagcttt tagttttcca tcttctagtt ctaatatagt tgtacataac tcatccagaa
     7141 aacgtctatc atgagatatt aataataatg ctccctcatg ccttttaaac atatcttcca
     7201 aaagcccaat gcttttttta tctaagtttg aagttggctc atcagctatt atgatatttt
     7261 tcttattact cagcgcatct gctattttta acttaacctt ttcccctcct gataagtatt
     7321 cttcaaattt atctggagca tttaaaagac tcttctcctt actatgacta catgtttcaa
     7381 tattattttc actctgactt atataagaat agctttctgt aaggtaagta tatccttcat
     7441 caatttctat ttcacccaat attgctttta ataatgttgt ttttccttga ccatttgaac
     7501 caactagacc tattttgtca ttttctaata tttcaagttt atctatatcc aaaattaatt
     7561 tatctgcata atatttcttt aaattctcta cttttactaa caacataaaa aatccctcct
     7621 taacacactt aaatataagg agagattcta taaataggca taacaaataa acgtatagac
     7681 tataatctac tctccttaat ttaagcattg caaataaagc catcaaaata ggcttttcaa
     7741 aaattacaat actttaaatt ataaaaaata gattatattc tcattgtcct agttaccttg
     7801 ccctttcttt catttatttt taatcattat atcaacatta attacaaatg tctatagaaa
     7861 attaactaat tttagttatt caatatacaa tttattcttt aaatttattc ttcttcaact
     7921 ttaaaatcct gattatttaa aattctgttt aatacaattc caacaattgc agctaaacta
     7981 agaccagtta tctttactgt acttgttata gggattccaa tagaaatgcc tttattagat
     8041 aaatatgttg ttccaagacc tattaataat ataactgcta ttattaatat atttatctta
     8101 ctttctacac aagaactgtc ccttatagtt tttaatccta cataagttat cattgaaaat
     8161 agcattatac taattccacc cataactgaa cctggtatac tttgaagaaa tccaccaaac
     8221 tttcctacac atgcaagcaa tatagcaaat attgctgtac gtctaagtat tgaagggtca
     8281 tagtttttag ttatagcaag tactgctgta ttttctccat aagttgtatt ggctggtcct
     8341 cctaaacacc ctgcaactat agtcgcaaat ccatccccaa gtaatgttct atttaatcct
     8401 ggattttcta taaagttttt acctactaca gcaccattag tagtcatatc tcctatatgt
     8461 tccataaata cagctaatac aactggagat ataatcacaa ttgctcctaa actgaactta
     8521 ggaactgtaa agttcggtat tgcaaataaa cttgcttctg taatagtagt tatatctact
     8581 aaccttaaca gtaaacagat tatataccca gaaaaaacgg ctattaatat tcctaattgt
     8641 tttataaatc ctctaccaaa cttattggtt aaaattgcta ttgctaaagt tataaatgct
     8701 attacaaaat tagctgaagc catactaaaa gctgttggca ataaatttaa tcctataact
     8761 actatcatag ctcctgtaac ttgtgcagga aaatataatt taattttatt tacaccaact
     8821 atttttacta taatcgacat tataatataa attaatcctg ctactattat accaccttga
     8881 gcatatgcta aatctccacc atacgcctct ttagctgcta gtataactgg tataaacgca
     8941 aaacttgaac ctaaaaaggc aggtactttt ccttccgtac aaaaatgaaa tatcaatgta
     9001 ccaacaccag cacagaaaat tgcaactgac ggattaaaac ctgtcaaaat gggaactaat
     9061 actgtcgctc caaacatggc taatagatgt tgtaaagaca ttattgtttt tttcataaaa
     9121 acctccaaaa ttattatata taaataaatc ctctgtttaa ttaatatctt ttaattaatt
     9181 atcattccct agattctatt ttttaacctc tatatactta ttatataatt tatcattatt
     9241 ttacatttta cgacatcaaa aactcaaata aaggagaata aaaactacat taagactcac
     9301 atccataaaa ttataatatt tattttgtgc aatctatgta agctttatcc tcctccctga
     9361 ctattcaatg ctaagctttt atataatact taaaactaat tattctttga ttgtaactga
     9421 atcttctcca tcaatttcta ataaactgac agatataatt tcatgtctag aagtaggtac
     9481 attttttcct acataatctg ctcttatagg caactctcta tgccctctgt ccactagaac
     9541 tgctaattgt attgatttag gtcttcctat atctataatc gcatctagtg aagctctcac
     9601 agttcttcca gtgtataaaa catcatcaac caaaactact gtcttatcat ttacattaaa
     9661 atctaaataa gttcctttaa ctacaggttc aacatgaatt ttttccaaat catctctata
     9721 taaagttata tcaatatctc ctgtatctac ttttgttcct tctatttgtt ctattttctt
     9781 agaaattcta tttgcaattg gcacacctct agtttttatt ccaactaaaa ctagattttc
     9841 tacaccttta tttctttcaa ttatttcatg acttattcta gtaattgctc ttgctattgc
     9901 cttttcatcc attaattgag ctttttctac catatcataa ttattgtcaa agatataata
     9961 tatctatttg acaaatctac tcctttcttt taatttttta taaatttatt tttatttaaa
    10021 atttcaattt aaacaacaaa aaacttctca tcacaagaca agaagctctt atataaaaaa
    10081 tatacagtgt aatttagcaa ttgtcttacg atatctctgt atcgtttaaa gataattttt
    10141 taatctatca actcaaatta tatatttata tttcaaattt ttcaagcttt tttaaacaaa
    10201 atactttaat ataagatatt ttatttaaat ttcactaaat ttatttactt atataaaaac
    10261 aaaaaatgat ttataagtat tatacaaatg gttattttat cttcttaata gcttccttaa
    10321 aatactcagg taattctgaa ttaaattcga tatattcctt agtggtagga tgtataaatc
    10381 ccaacttctt agcgtgcaaa gtttgtcctt ttaatttaaa tttagtttct tttggaccat
    10441 atacaaaatc acctaaaagt gggtgattta tagacaaagc atgaactctt atttgatgag
    10501 ttctaccagt ttcaagccta gctctcatat gtgtaaactt atcaaatctt tctacaactt
    10561 caaaatgagt aattgcattt tttccatcct ttactacagc catttttaat ctatccttag
    10621 gatttctccc tataggttta tcaacagtta ttttatcttc tttaaccaca ccatagcata
    10681 taaattcata ctctctagtt atagtatgtt cctttaactg ttctgctaga aaattatgtg
    10741 cataattgtt cttagctatc ataagaagac cagaagtatc tttgtctatt ctatgaacta
    10801 taccaggtct aataactcca tttattgacg acaacttatc tttacaatga tataaaatcc
    10861 catttactag tgtattttca tagtttccag gagcgggatg aacaaccata tcctggggtt
    10921 tatttattat taagacatca tcatcttcat atacaatatc tattggaata tcttgaggta
    10981 aaacctctaa tactttaggt tctggtatct ctataacaat attatcttcc tctttaacta
    11041 gatatttagc tttttctact ttattattga cctttacttt tccatctttt ataatttttt
    11101 gtatataact tcttgacata tcacctaatt gttctgctag ataaatatca agtctaactt
    11161 cttcttcctc ttcaagtact aaaaactctt ttacttcatc cattatatca cctactttta
    11221 ctttcaaaaa ataaaacata tatacataaa aacacagttc ctacaactac aaatacatca
    11281 gctacattga atacatattc ccatataact ctaaagtcga agtaatctac cacaaaacct
    11341 aatcgtactc tatcaattag atttcctaat gcaccagata taattaatat aattcccaac
    11401 cttccaaata tatgtacttt ccttgtatta agatagtata atccaaatac tgttgcaagt
    11461 aatgcaacaa ttataaatat ccactgatta ttttgtaata atccaaatgc tgcacctcta
    11521 ttttcgacat aagttaaatg aaatacatta tttattattg gtattgttga aacatctacc
    11581 aaattattca atacccatat tttagacagt tggtctaagc ctatgagtag aattattatt
    11641 aatatatata gcaatgattt ttccccctat aaattattta aaaaccaatt ttatttgaaa
    11701 cacactatta ttattataca aaaaaaatgc tctaaagagc aactttatta aaattgtatt
    11761 ttcttaataa agctagagca aaatttaaaa tagtaccatc ttttttatat tgcagattgc
    11821 tacatattta aaatcatatt attatgtata aaaaatttaa aaataacata ctaattaata
    11881 ctttttgtgt gttttataga ttaaagatta atgatgtctg tactttaaac ttacaagcac
    11941 cactaatctt caaacaaata tttttacttt tgcacttgct tatttttatc taaatgttgt
    12001 ataagttcag atactgttac gaatttatat cctctctctt ttaatttagg aattactact
    12061 tctaaagctt gtatagtttg tgatttatca tttcttttag tgttataatc atgtaaaagt
    12121 attatattac cattttggac ctttgtaaga atagtattta taattgtatc tacaccaggg
    12181 ttagaccaat ctctagggtc taagtttgac caaagaatta tattcatgcc tttagaaact
    12241 ataatatcac agacactttt actcatggct ctatatggag gtctaaatat ctttggttct
    12301 actcctatta cactttttat aatattttgt gtatctgtta tttctttttc aacactacca
    12361 taactatttt ttgcaacatt tatatgtgta aatgtatgat tccctatttc atgcccctca
    12421 tcatattgcc tttttaataa ttctttgtta tattctactt tttctccaac tacaaaaaaa
    12481 gttgctttta cattattttt ctttaatata tcaagtattt gtggtgtaaa atcctcatct
    12541 ggaccatcat caaaagtcaa tgctagaatc ttttcatcct catttccttt ttttattatt
    12601 acatcttcat aatcgtcttt tatattgtca tttaaagtat aggtttcttt tgaactattt
    12661 tgattatgtg tattatggtt atatgtatat acacctacta tggataataa acatatacat
    12721 ataccccata ttgctttctt catgtcatta cctcttttct aattcattct aacaatatat
    12781 gatgtcaggc ttctaatctc tcaatgttcc atttttattt aaatcagtta aatttgttag
    12841 gtcttctgaa taaaagttag aatcttctac atcaggattt tgcattgcat cacctaataa
    12901 agcattttct ttttttgcac aattgttaca taaatttgta tcaggtaata tatctaatct
    12961 ttctaaatct atttgattgt gacataaatc acaaaaccca tatgttccat tttctatttt
    13021 agataatgct gaatctattt gatacagtct tccttcttca tgatttatta aactattttg
    13081 catattttgc atatataaat ccgttcccat atctccaata tgattgtcat aactagataa
    13141 ttctcctgat gaatattttt cactagtatg tttagtagtg tctccaaaca aggtattatc
    13201 tttcatatct acgactaaat tagttaactt ttctctttct tttaataatt tatctttgta
    13261 ttttttagta tccattttca acacctctaa ttttaatatt tatatcataa aaatagtatg
    13321 tggaaaacta aattaaaaat acaataaaaa acaagatttg attttttcaa aaaaaattgt
    13381 aggtaaactt aaaattcacc tacaaattct ttacatatat ttttaatata atgtttttag
    13441 tttttgataa taattacatt aacttgctaa tttatgagca ttttgctctt ttatatcctt
    13501 agctttttta aaaaataaaa gtccaaggat tattgcaact acaataccta ttccattaga
    13561 tatattaact ggaagtctaa atccttcttt tgcctgcatt atataactaa atgttactac
    13621 agtcataaaa agtgctggca atgtagttat gtaatgattt ttatttgctt tgactaaata
    13681 tgcagaacca gtccacaaca ttatcatagc aagtgtttga tttgcccaag agaaatatct
    13741 ccaaattata ttaaaatcta taaatgtaag ggcaacccct acagcaaata atggcaaagc
    13801 tattaaaaat ctatttttaa ctgcatcttg attataattc attgcatcag ctattgttaa
    13861 tctagcactt ctaaatgctg tatcccctga agttatagga catgctacaa caccaagtat
    13921 ggccaaagcg cctccaatag gtcccattaa tccaacagat actgtattta caacagttgc
    13981 tgctccacca tcattaaata ttacattaag ctgaggtata ccatggaaat atgacatagc
    14041 tgctgctgcc caaactaaag ctattatacc ttctgcaacc attgacccat aaaatactgg
    14101 tcttgcctca ctctctttct caacacatct cgccataagt ggtgattgag ttgcatggaa
    14161 acctgatatt gctccacatg ctattgttat aaataagtat gggaataaag gtgttccatt
    14221 tggattcata ttttgtaaat ttatatttgg tatgttatac ccctttatta ttaatcctcc
    14281 agctacacct actgccataa ttaataatgc tgctccaaat aaaggatata ttttaccaat
    14341 caatttatct attggtaaaa cagtagccat aatataataa agtattatca ctccaaccca
    14401 tatttgtgcg tttacacctg tcaatgtctt taataatcct gctggacctg ttacaaatac
    14461 aactccaacc aatactaaaa gaataacact aaatattctc attgctattt ttgcaccatt
    14521 tcctagataa agacctacca actctgatgc agatgtacct ttgttcttca ttgataaata
    14581 tcctgaagca aaatcgtgta ctccacctgc aaagattgtt ccaaacacaa tccacaaaaa
    14641 tgctgatggt ccaaataacg ccccttgtat agctccaaat atcggaccta accctgctat
    14701 atttagaaac tgaattaaaa agattctctt ccatttcatt ggcacataat ctactccatc
    14761 cctacaagct atagctggag tttggttttt gtcattgatt ccaaaaacct tttctacaaa
    14821 agtaccatag ataaaataac ctactataag tactactatt gaacctaaaa agcttaccat
    14881 aaattcatcc ccttagtttt tatataattg ctttataaat ttttatgagt ttaagaaaac
    14941 tatttccagt tcaaatagta atactttttt atgctaatat aaatattaat tacattaagt
    15001 gcatatttaa gtacatgaaa tgactttata tatgcattaa cagcctaaat tcttttactt
    15061 tgcttctgct tacagttact tcatagtcac tattttttaa tttcaatatg tatgtattgt
    15121 taaaccatgg tataatttct tttactttgg ttaaatttac gatataagac ctatgtgttc
    15181 taaaaaatgt tttatcattt ataagattct ctacttctga tatgccttct tttaatataa
    15241 acttttcttt ctcagtatat ataaatgtct gcctttcatt agcttcacaa taatatatat
    15301 catcaatatc tattacaacc aatttatcac ctttccaaag gctaatttta tgtgttattt
    15361 cttctatctc ctgatttact gtctcttttt tatatttata taaattacta ttgctattag
    15421 acagttcaat atcactcatc tcactttttt ctaatttatt caacatagaa ataattctct
    15481 cttcagaata tggcttcagt acataatcaa atacatctaa agaaaaagca tctactgcat
    15541 aattttcata cgctgttata aaaacaattt taggccttga tttaaactga tttaatgttt
    15601 ttgcaagaag cattccatcg agatgaggta tatttatatc caaaaaaatt acatctattt
    15661 tattttcctg aataaagtct aacacttcta taccattagt aaattcattt actacctcta
    15721 tgctactttt attctcaata aaatatctta attcctttct tgccggaaat tcatcttcca
    15781 ctattatagc tctcatactt ctactccttt acataaaata ctatcaaagt cccattgtct
    15841 gttcttctta tattaagccc tcttccatac atcagtttaa gccttaaatg tacattataa
    15901 agtccaatct tattttctgg catattatct tgatatacat tatttataat ttcctcactt
    15961 atacctatac cattatcctc tattgatatt ctcacagtat tttctaatga ataaggtaac
    16021 ttttttatgg atattttcac tacaccagct ctaccacttt ctaaaatacc atgtattatc
    16081 gcattttcca ctaatggttg tataatcaaa cttggaatct ttatatttac atcttctata
    16141 tcataagata cagttagtaa ttctccaaac ctagcttttt ctatctctac aaaagattta
    16201 acttgttcaa tctctttatt tatatcaatt aagttatcac tatattcaag attatatctt
    16261 agataagttg ataaatttat tattaactct ctagccttat ctggactaaa tcttataaat
    16321 gaagctattg tgtttaggga attaaacaaa aaatgcggat taatttgtct ttgaagtgct
    16381 tttatttctg ctttatttct cgcttctata tcttttttat ctttctcaat cccctctact
    16441 atagatataa caaatcctat tccaatctgt ccaacaatta aaggtaaata tatcccaaca
    16501 attatttgaa ttgctaaaga atgtggatat gatattaagt atataagtag tatagtaaaa
    16561 ctttcagaaa tcatacctac caatatacca tacattactc tatgttgctt tggtatcctc
    16621 ttatatagaa atcctgataa tacacctgct agtatacttg ataaaagaca tggaacagat
    16681 gttatccccc caatatccat aaaatatcta tgaatccctg aaaaaactcc tgctgtaata
    16741 cctaccattg gaccaaataa aatgccacct gatactatag atattattct agtgtttact
    16801 attgacccca tataatttat accactataa gttccaaata tggctaaaaa agtaaaaact
    16861 aatgctatac agaccaagtc attaaaacta tattcttcct tttgaaatat ctgcttaaat
    16921 atttttaact ttgttattag aagaaacagt actacaatga ggcttgtctt ttctattaat
    16981 gcgattaaaa ttgatgtttt catattccac ctttaaaaaa taaattttta cacactctaa
    17041 atttattatg tttgttgtaa cttatatatt tgaagtaatc ctatctcgac attttccaca
    17101 tttaaccttt taattaaata aaaaatggat atacttctat taacaactaa tactagctga
    17161 ctaacacaag cttgtaataa cttgtagtta tcattaactc tacaattcta gtatttaatt
    17221 ttatacttta gaaatctctt ttcttagaag taatactttc gaatattata taatacaatg
    17281 ttatgtagat tcttactcat acaacatatt tctttaaaaa ttttatacta ttctttgttc
    17341 aaatatctca tttgatttaa ttgtgctaaa cacacattat catctctctg ttatatattt
    17401 attatatagc cgtatatcga taaagtaaat aaatcctata tttaaaatca tatgtttgat
    17461 agtaaatcat tataatgtat atttaccata ataatttgcc aaccttgaaa gtcacttcta
    17521 taattagaag ttaaataatt cttctaaaaa aaatacctct aaattttatt ttctgaaata
    17581 catagttata actattttta tcacgtataa gaataaaaat tccag
//
LOCUS       DAEPOG010000076        17572 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEPOG010000076 DAEPOG010000000
VERSION     DAEPOG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.076"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..835
                     /locus_tag="KR432_002082"
     CDS             <1..835
                     /locus_tag="KR432_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0640613.1"
                     /translation="KNFEKISLGDNLSYVLSSIGKPGRIDISEYGFDWYVYNQYGKEF
                     AMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKITPLKYKRKGNTRYIINSEN
                     QYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFKKLYPDDREELKKCFELEVI
                     DLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFDHINKKNETPFDRMKREGIV
                     YTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNIGVGVIFGGSYKTYYTQNFY
                     K"
     gene            863..1582
                     /locus_tag="KR432_002083"
     CDS             863..1582
                     /locus_tag="KR432_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0640614.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            1606..2190
                     /locus_tag="KR432_002084"
     CDS             1606..2190
                     /locus_tag="KR432_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBH0640615.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            2192..3541
                     /gene="pabB"
                     /locus_tag="KR432_002085"
     CDS             2192..3541
                     /gene="pabB"
                     /locus_tag="KR432_002085"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBH0640616.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            3531..4274
                     /locus_tag="KR432_002086"
     CDS             3531..4274
                     /locus_tag="KR432_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBH0640617.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            4308..5009
                     /locus_tag="KR432_002087"
     CDS             4308..5009
                     /locus_tag="KR432_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640618.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            5035..5607
                     /gene="folE"
                     /locus_tag="KR432_002088"
     CDS             5035..5607
                     /gene="folE"
                     /locus_tag="KR432_002088"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH0640619.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            5673..6464
                     /gene="folP"
                     /locus_tag="KR432_002089"
     CDS             5673..6464
                     /gene="folP"
                     /locus_tag="KR432_002089"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0640620.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            6475..6840
                     /gene="folB"
                     /locus_tag="KR432_002090"
     CDS             6475..6840
                     /gene="folB"
                     /locus_tag="KR432_002090"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBH0640621.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            6833..7339
                     /gene="folK"
                     /locus_tag="KR432_002091"
     CDS             6833..7339
                     /gene="folK"
                     /locus_tag="KR432_002091"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH0640622.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            7320..8105
                     /gene="aroF"
                     /locus_tag="KR432_002092"
     CDS             7320..8105
                     /gene="aroF"
                     /locus_tag="KR432_002092"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0640623.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            8360..10150
                     /locus_tag="KR432_002093"
     CDS             8360..10150
                     /locus_tag="KR432_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0640624.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            10168..11334
                     /gene="rpoD"
                     /locus_tag="KR432_002094"
     CDS             10168..11334
                     /gene="rpoD"
                     /locus_tag="KR432_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH0640625.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            11499..12188
                     /locus_tag="KR432_002095"
     CDS             11499..12188
                     /locus_tag="KR432_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0640626.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            12202..13299
                     /locus_tag="KR432_002096"
     CDS             12202..13299
                     /locus_tag="KR432_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH0640627.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            13361..13933
                     /locus_tag="KR432_002097"
     CDS             13361..13933
                     /locus_tag="KR432_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0640628.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(13993..14460)
                     /locus_tag="KR432_002098"
     CDS             complement(13993..14460)
                     /locus_tag="KR432_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0640629.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(14846..15322)
                     /locus_tag="KR432_002099"
     CDS             complement(14846..15322)
                     /locus_tag="KR432_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0640630.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(15442..16533)
                     /locus_tag="KR432_002100"
     CDS             complement(15442..16533)
                     /locus_tag="KR432_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBH0640631.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(16526..16915)
                     /locus_tag="KR432_002101"
     CDS             complement(16526..16915)
                     /locus_tag="KR432_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0640632.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            17107..17553
                     /locus_tag="KR432_002102"
     CDS             17107..17553
                     /locus_tag="KR432_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640633.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
BASE COUNT         7153 a         1843 c         3133 g         5443 t
ORIGIN      
        1 aaagaatttt gaaaaaataa gcttaggaga taatttaagc tatgttttat caagtattgg
       61 taaacctggt agaattgata ttagtgaata tggttttgat tggtatgtat ataatcaata
      121 tggaaaagaa tttgcaatgg tcggacttga aaatgatgaa gtagtagcac tttatagtaa
      181 ttctattaat tcatgtgaaa atcaagatat aaaactaaat caagatagac aaacagtgag
      241 aactaagatt acaccattaa aatataagag aaaaggaaat accagatata taataaactc
      301 agaaaatcaa tatgatataa taagtaaaga aggtaagtat attacaattt tctacgatat
      361 tcatgaagaa aatagagttt gctcatattt aataattgat aaaagtactg aagatgagtt
      421 taaaaaatta tatccagatg atagagaaga acttaaaaaa tgttttgaac ttgaagttat
      481 agaccttgta aatagtgtta gaaatcaaag aggattaaac tcattgagat acagtgaaca
      541 ggcaactttg agttcaagaa aacatagtga agatatgaga gataataatt tttttgacca
      601 tataaataag aagaatgaaa ctccatttga cagaatgaaa agagaaggta tagtatatac
      661 ttcagctgga gaaaacatag cagcaggaca gattaatgca atatatgcgc atgaagcttg
      721 gatgaactct gaaggacata gaaaaaatat tttggggaac tataataata ttggagttgg
      781 agtaatattt ggaggaagct ataaaacata ttacactcaa aatttttata aataactaaa
      841 atatttgaaa ggaagttaaa aaatgacatc atggaaaaaa aagacagtat ataagtgctt
      901 aatagcagta gtcttgtttt gtggaatagt gttaatatct aattttagta aagtttctgc
      961 tttgatgatg gatactaatg gaaatgtttt gattaaacat ggttcaaggg aaaaaaagct
     1021 aatagcaatt acctttgatg atggtcctca tcctaaagaa actagtcaag ttttagatgt
     1081 attaaagaaa tacaatgtaa aagctacttt ttttatagct ggaaaacatg caaaatggta
     1141 taaagaacca ttagtaagag caagtaaaga aggtcacgag ataggaaatc atactttcaa
     1201 tcatcctgat ataagtaatt taagttctag tcagatagaa gaggagattg ttaagtgtga
     1261 agacattttg aaagaagtaa ctggaaaaaa acctacatta tttagaccac catttggaag
     1321 ttatagagaa aaagacttaa tagaaattgc aaaaaagcat gactataaag ttgttttatg
     1381 gacaggtgta gatgtgaaag attggaaaaa tccaggagct aatagcattg cagataaaat
     1441 aattaataaa gtaaaaaatg gagatataat acttttgcat gattatgcaa ctaatgatac
     1501 agttgaagct ttagatatgt ttattccaaa aatgattgaa aaaggtttta aatttgtaac
     1561 agtttctgag ttgataaaat aatttttagg ggggagattt acagtatgat tcttatgata
     1621 gacaattatg attcttttgt atacaaccta gtccaatata tagaagaatt aggggaagca
     1681 gttgtagtaa aaagaaataa tgaaatcaaa ataagtgaca ttgaagaact aaatccagag
     1741 gtaattgtgt tatctccagg accgtgttct ccaaaagaag ctggtatttg catagatatt
     1801 gttgagcatt ttaaggggaa aaaacctata ctaggaattt gtttaggtca ccaaactatt
     1861 ggatatgttt ttggaggaga tattataaaa gcacaacagc ctgtgcatgg aaaagtatat
     1921 agtatcaacc atactaataa aggagttttt aggggactta aaaatccttt aaatgtcact
     1981 aggtatcatt cactaataat tgattccaat acagttccaa aagaattaga aattacagca
     2041 attactgata aaggggaaat aatggggatt aggcataaga aatatttaat agaaggagta
     2101 cagtttcatc cagaagctat tttgtcagag tatggtcatg aaatgcttga aaattttata
     2161 acagaagcta gagaaagggt acatatgtaa tatgataaga gagataaata ctaaattaaa
     2221 ttcttttgag atatttacta tttttagaaa tgaacatgac agttttatat tagacagtgc
     2281 tatggataaa gaaaaattgg gaagatattc ttttataagt agccaaccat ttaaggtatt
     2341 aaagtataag gatactgatg aaaatccttt agaagtttta aaagaagaac tacataagta
     2401 tagggttgta aatgatacta atcttccatt tgtaggaggt gctgtgggat atttatctta
     2461 tgatttaggt aattacatag aaaatttacc tagaactgct gtagatgata ttgagatgcc
     2521 tgatatgtat tttggatttt ataatcatgt aatagttata gaccatcttg tgcaaaaaac
     2581 ttatatagct actccaaaca tagatatcga attagaagaa aagataatag atgatataga
     2641 acaaagaata ttaaaagaag agaaaaaagg tatagatagc atatgttatg aagaaaaaga
     2701 agtaacacct ataagactta aatctaattt tacaaaggaa gaatttaaaa atgcagttca
     2761 aagtgttaga gagtacataa gacaaggaga tatatatcaa gctaatttaa cacaaaggtt
     2821 cagtggagaa acagaactta caagctttga attgtatagg gacttaagaa gatttagccc
     2881 agcaccattt ggggcattct taaactttga agatacacat atattatcaa attctccaga
     2941 aagatttata agatgtgtta ataagagaat agaaactaga ccaataaaag gtactcgtcc
     3001 aagagggaaa gataaagaag aagatttaag acttcaacaa gaacttagaa atagtgaaaa
     3061 agatagagct gaactactta tgatagtaga tttagaaaga aatgatattg gcagaatatc
     3121 taaaactgga agtgttaaag ttccagagct atttgtaatt gaaccatatg caaatgtaaa
     3181 tcacttagtt tccacagttg taggtgagtt aaaagatgat aaagatgcta ctgatgtaat
     3241 aaaagcaact tttccagggg ggtctataac aggagcacct aagattagag caatggaaat
     3301 tatagacgaa ttagagccaa cgcaaagaaa tgtatatact ggttcaatag gttatatagg
     3361 ttttaatggt gatatggatt ttaatatagc aattagaaca ataatcaaaa atgacaaaaa
     3421 agtctatttt caagttggag gaggaatgac ttgggattct gacccagatg aagaatatca
     3481 agaaacactt gataaagcta aatccataat gaaggctttg aggggatatt atgaagaata
     3541 atgtaggttt tgatagtagt ttaagtaaat ttggaattgg tttgtttgaa actattagag
     3601 ttaaaaatgg aatagcagta gatttagata tccatataga aagaatgcta agttcaataa
     3661 attgtttgga tttagatata aactataaaa aagatttctt gataaatgaa attgttacat
     3721 atataaaaaa agaaaatgtc attaataaag cacttagaat tactgttttt gatgaaggtt
     3781 ataatatatc tattagggat attccatata atcaagagac gtatgagaag gggtttaaat
     3841 tagctatatc gccaatagtt agggggaata gtttaattca tagacataag actactaatt
     3901 attttgaaaa catatatacc aaaaatatcg caaataagac tggttataat gatggtatat
     3961 ttttaaattc agaaggtgta atattagaat gttctatgag caatattttc tttataaaag
     4021 gagaaagagt gtacactcct agtgatagat taccgatatt aaatggaata ataaaaaaaa
     4081 ggattataga gatttgtgac gaattacata tagagttgat agaaaatgaa attaatatat
     4141 cagaaattag tagttttgat tttgtttttg tcacaaatag tcttatgggc gctataaagg
     4201 ttacagaaat tgacaaaata aaatttaata aagaaaatgt tgtttttgat aaaatagtga
     4261 agttattaga ataaattaaa atattaacaa tttgttggag acaaaagatg ataggttgta
     4321 tttatgaagt atacaatgaa gagatgacta aaataataga aaaccaaaac actttggctg
     4381 tatatttagt aggttctagt aaagacgtag attttactct ttatgatgta gagataaacg
     4441 atattgatgt gttcgttttt gtaaaagaag gggaaaaaca agagagaata ctgtttaaaa
     4501 aaaagggaat agaatttgat gtaaattact tttcaaaaga tggtgtaaaa aaattattat
     4561 caaataggga atatttcttt gtgaaagaaa tgaaagatgc taaagtactg tttgataggg
     4621 agaatatatc aaatattata aaagatataa gtagagatat ttacttagaa ggacccagta
     4681 aaatgtcatt agaggaaaag tcttttataa agcaagatat tggagcaaaa atatcgaatt
     4741 taaaaaataa agaaaaattt gatgtttttg aatatcattt tttgacgaat ttatatttaa
     4801 aagatataat aattgggtac tttaatataa atgataaatg ggtaccaaaa gacaaaaaat
     4861 tgctaaaagt tttaaaaaaa gaaaataatg agctttttga actagcgtca aaagtaagtg
     4921 aaaattatga ctatcaaaga cttttagatg tttataatta tatttttaaa gaaattgaga
     4981 caaaagaggt tataaaacta atcttctagt cacaaatcaa tggggggagt ttaaatgaat
     5041 aaagttgata aagaaaagat acaacatgca gtaagagaaa tactagaggc aattggagaa
     5101 gacccagata gagaaggtct aatcgagact ccaaatagag ttgctaggat gtatgaagag
     5161 atattctctg gtcttagtga agaaccaaga gaccatttaa aagttctttt tgcagatgaa
     5221 aaacatgaag aattagtatt ggtaaaagat attccatttt attcatgttg tgagcatcat
     5281 ctagttcctt tctttggaaa agctcatatt gcttatttac caaaaggagg aagacttaca
     5341 gggctttcta aacttgctag agtaatagat acattggcaa aaagaccaca attacaagaa
     5401 agaatcacta aaaatgctgc agatataata atggaagaat tacaacctta tggtgtctta
     5461 gtagtagtag aagctgaaca tatgtgtatg acaatgagag gtgtaaaaaa accaggttca
     5521 aaaactgtta catcagcagt gagaggaata tttgaaaagg acattgcgtc tagagccgag
     5581 gctatgagtt tgattactat gaagtagcaa tttattatag atataatcat ttattgagat
     5641 ataaaattta atttaaggga gtgtcattaa ttgtgtttga ttatggtaaa agaacttata
     5701 taatgggaat attaaatgta actccagata gtttttctga tggaggagat tttaataact
     5761 tggacatagc aatacaacat gcaaaagata tggttgacca aggtgcagat ataatagact
     5821 taggaggaga gtctacacgc ccaggtcata gctatgtaga ctcagacgaa gagctaagaa
     5881 gagtaatccc tgtaataaaa aaacttaaac aggaattgga gataccaata tcaatagaca
     5941 cttataaggc agatgttgca gaagaagcat taaaattagg tgtcactatg gtaaatgatg
     6001 tttggggtct tagaaaagat aaaaatatgg cttctgtaat aggaaaatat gatgcagaag
     6061 tgtgtataat gcataatcaa gatgggacaa actatgacaa agatataatg gagtcaataa
     6121 aagacttttt taaggtaagt atagaaatgg caatgagttg tggtgtaaaa aaagaaaaaa
     6181 tagtacttga cccaggcgta ggatttggca aggattttga acaaaatatt gaagtattga
     6241 gaagattaaa tgaattaaaa gatttagggt atcctatttt gcttgggaca tccagaaaat
     6301 cagtaatagg aaaagtactt ccagttgagc ctaaaaaaag attagaaggt acaatagcta
     6361 ctactgttct tggtataaga gatggcgtag atatagttag agtacatgat gtttatgaaa
     6421 atttaatggc agctagaatg actgatgcaa tatatagaaa gtaggtataa tataatggat
     6481 aaaatattac ttagtaatct aggattctat ggatatcatg gtgttcttaa agaagaaaat
     6541 tttctaggtc agaaattctt tgtcgacatg gaactttata ttgattcaag agaagcaggt
     6601 cttagtgacg atataaataa atctgtaagc tatgccgagg tttacaatgt tgttaaagat
     6661 attactgaaa ataaacaatt taatcttttg gaggctttag cggaaaatat tgctgaagaa
     6721 gtcttgaata aatttatact tataaatgga gttatggtca gagttagaaa gcctgaggct
     6781 cctgtaaatg gtatttatga ctattttggt gttgagataa ggagaacaag agatgaataa
     6841 ggcttatttg ggaataggta ctaacatggg tgatagattt gataatttat caagagcatg
     6901 tgaacttttg aaaaatagtg attctatata taaagtaaag gaatcaagtt tatatgaaac
     6961 aaaaccatgg ggatatacag aacaagcaga ttttctaaat atgtgtgtag aaatagaaac
     7021 agaatttgaa ccttatgagt tgttagaata ttgtcaagaa atagagagag aactacacag
     7081 agagagaata gttcattggg gaccaagaac tatagatgta gacatactat tttttaatga
     7141 tgtagcttcc actgatgaaa gactaacaat acctcatccg aggattcaag atagagcttt
     7201 cgttttgata ccacttatgg atttaaatga agaacttata ataaatgaaa aaacagtaaa
     7261 agaacattta aacctcctat ctgctgaaga aagagaagaa gtaaaggagc ttgtaggtta
     7321 tgaaagaaaa cctatttaat aataattcaa agatagagat aaatacaaat aacaaaaaga
     7381 taactgtaaa aaaagataaa cttataatag ctggaccatg tgcaatagag agttatgagc
     7441 aacttttgga gactgctaaa tttgtaaaaa gtcagggagc aaatatttta agaggaggag
     7501 cgtacaagcc aagaacatcg ccaaattcat ttcaaggatt aaaaaaagaa ggtcttaaaa
     7561 tattaaaagc agttaaagac gaagttggta tggctgttat tacagaactt atggatgtaa
     7621 gagatatcga cgaactgtat agtataagtg atataattca aattggttca agaaatatgc
     7681 aaaactttac tcttttaagt gaagtgggaa aacaaaataa acctgttatg ttaaaaagag
     7741 gaatagcatc cacaattact gaatggatag gtgcttctga atatatagca atagaaggaa
     7801 atagtaacat tataatgtgt gagagaggta taagaacgta caatgattat actagaaata
     7861 cattagattt agctgcggta cccattattc aaaaggaaac aggtcttcct gttgtggtag
     7921 acccaagtca tgccactgga gttagatatt tggtaaaacc tatgtcactg gcatcttttg
     7981 catgtggagc agatggaata atggtagaag tacatcctga cccagaaaat gctttgtcag
     8041 atggtgctca atctctttgt tttaatgaat ttgaagattt aatgaaatca attaataatt
     8101 attaaaagag gcaagagcct cttttttatt cgtaatcatt gctaaggtaa atttataagt
     8161 tataatttga tagttagatt atttaatata aataaagatt taatattgat agaattaata
     8221 taaaaaataa aaattttttg attaataaga aggatatcaa taaaaaaagt cgaattgtgt
     8281 atattgagtg attgacaaat ttagaatata gtgtagaata gaaatgttaa cacaaaaaat
     8341 tacaccagaa ggtgataata tgaatgatat aaaagacatt attgaagaaa taaaagctag
     8401 atgcgatata gcaagtataa tatctgaata tatgaatata aaacaatctg gagctaatta
     8461 caaagggtta tgtccatttc atggtgaaaa gacaccttct ttttatataa atacatcaaa
     8521 acagatttat aagtgttttg gttgtggtga aggtggagac gtaataaatt ttgtcatgaa
     8581 aatggaaaat ttggatttta tggatgctgt aaaaatttta gcaaataaat gcggtataga
     8641 aattaacact aacatgaatg aagaaactag gattaaaata gaaaaatcta agaaatttca
     8701 agatatccat acggaagcag ctagatttta tctttcaaac ttattgggaa gcaaaaatct
     8761 tgggtatgaa tatttaagaa taagaggact agatgataaa attattaaga aatttggttt
     8821 aggattttct ttggattcat ggaattcact tatgaatact ctaataagta aaggatacaa
     8881 aaaacaagac ttattggatt gcggtctaat tgcaaaaaat agagatggaa ctaattgcta
     8941 tgataaattt agaaatagag ttatgtttcc tatatttgac tatagaggaa acataatagg
     9001 atttggggga agagtattag atgattcttt gccaaaatac ttaaattctc cagacacatt
     9061 aatttttaat aaaaaacaga acttatacgg attaaatttt gctagaaaaa atttagaaag
     9121 taaaactata gtcttagttg aaggatatat ggatttaatt tcgctttatc agtatggtat
     9181 aaaaaatgta gtagcaaccc ttggaacagc cttgactgaa caacaaggca ttttaataaa
     9241 gagatatgcg gacactgcta ttatatctta tgattctgat gaagcaggaa taaaagcgac
     9301 tttaagagct atagatatac ttactaaatt aggtatcaat gtaaaagtgt tagacttaaa
     9361 agatgctaaa gacccagatg aatttgtaag aaaatacgga cttagtgatt ataaaaaagc
     9421 tatggatgtt tctactcact acataaaata taaaatagat catcttaaaa aagaatttaa
     9481 cattcaaaaa gatgaagaac gagtgaagtt cgcaaaagaa gcttctaaaa ttataaaaca
     9541 attgacaagt cctgttgaaa ttgattttta tacaaagtat ttaagcaatc aaatagatat
     9601 taatgtcgag tctataaaga gagaagttta tggcaaaaat tacaataagc catataataa
     9661 caaaaatcaa aagaaaatag aagaaaaagt aattgaaaaa gtcgaagtca gacaagacgg
     9721 aaaacaatta gttgaagaaa ctctaataaa aataatgtta gaagacaaaa aaattagaga
     9781 aatagcgtta cttaaagtag aagaaagtga ttttttattg aaagaaagta aagaaatttt
     9841 aaattacatg attaaaaatc aagaattgga caaaataact attgacaaat tgaaaagttt
     9901 aaacatatcc gaagaatact taaaagaatt aaattcaatt tctttgaata gtataaactt
     9961 agagaacaca aaagaaatag aaggaataat aacaaatatc agaaaaaact cattagaaga
    10021 gcaaattaat agcttgttga gagaacaaca agaattagaa aataataatg atatgaaaga
    10081 ggtagatggt agagttatgg aaattgcttt aaaaatagtt gagataaata aaattttaaa
    10141 gagcttgtag agaaaggagg tttgagtgtg gaaaataaat cgaataaaaa agagttaaaa
    10201 aaggttactg ctaagacatt aatagaaaaa ggaaaaaagc aaggttctct gacacttgca
    10261 gagataatgg aagctttttc ggagactgaa cttgataagg atcaagtaga aaatctttat
    10321 gagactttag gaaatttggg aatagaaata acagaaacaa aaaattataa agctgatata
    10381 gatttttcgg ttgctgatga cgatttaagt ataggccact tagatgaaga tgcagaggca
    10441 atttcacatg atgactcttc tgcaatagaa atagaaactg tggatttatc tttaccaaaa
    10501 gggataagta tagatgaccc tgtaagaatg tacttaaaag aaataggaaa aattcctcta
    10561 cttaaaccac atgaagaagt agaatttgct agaaggatgc acgaaggtga tgagatagca
    10621 aaacaaaggt tagttgaagc aaacttaaga ctagttgtaa gtatagcaaa aagatatgta
    10681 ggaagaggta tgcttttctt ggatttaata caagagggaa atttaggtct tataaaagca
    10741 gtcgaaaaat ttgactatac aaaaggatat aagtttagta cttatgcaac atggtggata
    10801 agacaagcta taacacgtgc tattgcagac caagctagaa ctataagaat accagttcat
    10861 atggtagaga ctataaataa gctaataaga gtatcaagac aactacttca agaacttgga
    10921 agagatccaa aaccagaaga aattgcaaaa gaaatggaaa tgacagaaga taaagtaaga
    10981 gaaataatga aaatagctca agaccctgtg tctttagaaa caccaatagg agaagaagaa
    11041 gatagccatt taggtgattt tattccagat gatgatgctc cagccccagc agaggcagca
    11101 gcatattctt tattaaaaga acaaatagaa gatgtacttg gttcgttaaa tgatagagaa
    11161 caaaaagtat taaagcttag atttggtctt gaggatggta gagccagaac tcttgaggaa
    11221 gttggaaaag agtttgatgt aactagagag agaataagac agatagaagc aaaagcacta
    11281 agaaaactaa gacatccaag tagatcaaaa aaacttagag attatttaga ctaaaaagat
    11341 acatcattga tgtttttgtg ataaatgcca tgagataagt gatatctcat ggctattttg
    11401 tagtaaagat ttaattttat ttttgtagtt atttcagtct aatatctata acttggatta
    11461 aatcctataa ataaagggaa ttgaaaggag aattatattt gaaattaaca gatagattat
    11521 tgaaaatagc atcattagtg tcagatggta aaaaaatagc tgatataggg acggaccacg
    11581 gatacatacc agtatatttg ttaaaagagg gaaaagtacc atttgcagtg ctagcagatg
    11641 ttaataaagg acctcttgac aatgcacata aagaagtcat acagaacaat cttttagata
    11701 aagttgattt acgattgggt tctggtatag agatacttga aataggggaa gtagaagaag
    11761 tgataattgc tggtatgggt ggaatcttga taagtgagct tctcgaggct aaaaaagaag
    11821 tagcacataa tgtagaaaaa ttaatattac agccaatgca agcacaagaa gaacttagat
    11881 attacctttt aaacaacgga tatgaaattt tagaagaagt tttagtgaga gaagatttta
    11941 gaatatatga gataattgtt gccaaatata caggtaaaaa tactataata gaagatgaga
    12001 tatattatga ggttggaata aagcttttag aaaacaaaga ttctcttttt aatgatttta
    12061 tagaaaaaaa gataaagaca tatagttcta tagttaataa attagaaggg aaaaatgggg
    12121 aagctataga taagaagaga aaggaaagcg aagttgcaat aaaaaaactt gaaaatttaa
    12181 taaaataaat aggaggtata tatgctatta aagagtctaa ctagaaaaat tgaaaaaaag
    12241 tatcctctaa acttagcaga ggattgggat aatgtaggtc ttatagttgg agattttgat
    12301 atggatgtaa aaaaggtatt ggtatcttta gaagctaatg aagatgttat tgatgaagct
    12361 atatccaaaa atatagattt aattgtgaca catcatcctt ttatatttgg gaaaattaat
    12421 aaaattaata gtggagattt aaaaggcaga cttatccaaa aacttattaa aaatgatata
    12481 tcactttatt ctatgcatac gaattttgat atagcatttg atggtctaaa tgactatttt
    12541 atggaaatta tggaatttgg taattcaaag gttttagata taactaaaag cgaaaatttg
    12601 tataaattag cagtatacgt tccacataac tattcagatg aacttagaaa ggttttaagt
    12661 aattcaggag caggtcatat agggaattat agtgattgta cgttttcaat agaaggtgaa
    12721 ggacaattta agccattaga aggctctaat ccgtttcttg gaagtgtaaa tgatatagaa
    12781 actgtaaatg aagttaaaat tgaaactgta gtaccacaga agcttttagg tggggtaata
    12841 agttctatgt tagatgcaca tccttatgaa gaagttgcat atgatttgta caagttagaa
    12901 aataaaggtg agatatttgg actaggtaga ataagtaaac ttgataaaag tatgacatta
    12961 gaaagtcttt caaggaaaat aaaagagaaa ttaaatatga aacacataag agtagttgga
    13021 aaccttagta cagacataac gaaagtagcc gttgtaacag gagctggttc agaatttgta
    13081 aaaaaagcta agagacaagg tgcagaagta ctaattacgg gtgatgtaaa gtatcatgag
    13141 gcacaagatg cacttgatat tggaatgtgt atagtggatt gtggtcattt tgatacagaa
    13201 gatatattca aaaatgttat gaagagattt ttagatgaat ttagtgaaat tgaagtaata
    13261 aagagtaatg tatatttgaa tccatttagt actatttaga agtgtttgat taaaaaatac
    13321 tatataaaaa ataaatttga gtttaattag ggggataatg atgaaagtag cagtaatttt
    13381 tcatagtgtt tgtgggagta catatttatt agctagagaa tataaagaag tgcttgaaga
    13441 aatgaatatc gaggtagata tatttagagt aagtgacgaa gttgcaaaaa cacttcctca
    13501 atattatttg ataaattaca aagaatacaa agacgaattt gaaagtataa atgtaattaa
    13561 atctggaaaa gaaatattag attatgatgc tatatttatg ggttcaccaa catattatgg
    13621 aaatgtatct ggaccaatga agatgtttat ggatagtttt tctgatattt gggtaggtgc
    13681 tcctttgagt ggtaaaatat ttggttgttt tgctacagca ggttcacaac atggaggagg
    13741 agaattagct ttgcaagcta tgaatatatt tgctcaacat atgggaatga ctcttttgtc
    13801 agttccatgt tcagtaagag gaggttatcc agcgtatgga atacttcata tagcaggaga
    13861 taattctgat ataagaccaa atgatgatgc caagataggt ataagagatt acctaaaaag
    13921 acttaacata taagaatatc atataaaaat acctaaaagt agctgtatat acttttaggt
    13981 atttttactt aattatatac gtgaagcttt accagaaata tggtcaattt ctattttaac
    14041 aacagttgtt aagttcatat ccttctctat gtacttcatg ccttcttcaa agaatccttt
    14101 agaatatttt tttattattt ctacaagagc atttttcttt tcttcatttt ctacagtgca
    14161 agcttttcca aaaactatag cactagaata agtagtacta aatttatctg gaataacact
    14221 ttcattagca actactaaaa aagaaacttt gttgtttttc gatatattat ctaacttatg
    14281 tccatttctt gcacaatgaa agtaaataga atcattaaag tatacataat ttacagcaac
    14341 tccataagga taaccatttt cagatatagt ggaaaaagta ccaaattcac cattttttaa
    14401 tacttcaaca gtatcttctt ttgtcatttc tctttttttc aatctcattt ctttaaacat
    14461 aatatcatcc tttctaattt tgatgcttaa attaaatttt agcacattta taaagattat
    14521 gctatgtttt ttacaaattt taagaaaaaa atatagttta agataaaagg tagtgaaaga
    14581 gaagctattt ggaggtttat ttatgatatt attgttagag tagtacaaac aatactaata
    14641 aatttaatag ctggtttctt gttatttaag gtagtaagca tgtctataag aaggggaagt
    14701 tatcgagggt agaagttgtt atttataacg atattataaa gaaaatatta ttatataaaa
    14761 taataaatag taaagaggta tcaagttgaa tataatactt aatacctctt caaaattaaa
    14821 ttataaaagt gttgtgggaa ttgtcctata catggtaagg agataataaa gaatgtatct
    14881 ttaaagtctc aactaaacag caaaatttat ccaacaaaca tacaataaat gcttctttgt
    14941 atctaggtgg cttaacagta agtggccaca tgtgttttaa aattatgtcc gtttcttttt
    15001 catttatttg aaaaaataaa gttgcatttt ttaatgcttc tctagggtga gtaaatccat
    15061 gtaatccttt tctatcacct ttgtaatgcc aatcataaag aaaaaagtcg tgtagtaaag
    15121 ctcctctaat aacactttga atatcaacac gtaagtgcaa ttttttacat aatagataac
    15181 tataatatgc tactgacaaa ctatgttcaa gacatgaaac attgccatga tgaggaaatg
    15241 ttctcataag ttgaactttt tcagaagaaa gtatctcaga agcacattca ttaaaatatt
    15301 tgatttcagc ttttgacaac attatgaaaa tctccattct tagtaatata taaatactat
    15361 acttcaacga taatatgaaa tatttcttat cctacaaaat ataataccca ataatacaag
    15421 taagatacct tttatggtat tttaaagtaa gagagatttt attaggctaa acaatgaaaa
    15481 aattaaaagt aaagctatag caatatatag tatttttcca agaggattgc caaaatttat
    15541 agtccatcca ataccaaacc ttttattaac cattaatgat gggtcatttg gattattata
    15601 catgatgcct gctatccaat acttctcatc ttcttctgga gaataagatg agttgaattt
    15661 taaatttggg gaattaatat atgttgctat taaataaatt ataactaaaa gcgttgtaat
    15721 attcatagca gttgatagtt ttgtttttat tgataaaaga gtagtaaaaa actgtgttgt
    15781 cattataatc atcaatatta aaaacaaata accaatttta tttaaatatt taatgttttc
    15841 aactctactt tcttcaattc tatttgtatc tatcttaatc cttgacttta tggatccaat
    15901 ggaagttata tataataaaa ccattaaaca aaagtcagta ccgattaatt taaatacatt
    15961 aagaaatgat ttatctataa aaacatcagg agacccattt atgttccaat gagtggcaat
    16021 tgagtctggc aatttattat aattaaatgc tataaaaata ctcatcccca aagtaaatag
    16081 tatcggtatt aaatagatta ttttaaattt ttttattatt ttatttttct tatttataaa
    16141 atccatatct actattaatt ttgaatctac atctatatga cctaatttag aataaatttc
    16201 tgatttaagc ttcttagctt ttttatgtgt atgaatatat agtatactct catataataa
    16261 aaaacctata ataccaataa aatatgaaag ttccaattta ttaaatgtaa aaaccagaat
    16321 aaaaactaat ataaagtcta taataaaacc taaagataat agttttttaa attctttatc
    16381 taattttata aagtaattat aatttttatg ctcttggtct atagaaactc catagatgtg
    16441 ttttttacct acaaaatagg gcataaaata aaatgtagca tacatcaaaa ttagaactgg
    16501 aaatattgta aatagacatt caatcttact cataatattt cacctcatac ttgtcaaata
    16561 aacttgaact tagtgacaaa aattcttctt tactaatacc tgataaataa gattttgcca
    16621 ccaataattc caagtcaagt ttattttttt gacgagttgt attatctatt ttcattggta
    16681 gttgagctac aatagcacct tttcttctat caatatttaa gaatccctct tcttttaact
    16741 cattgtagga tttgttcact gtatgtaaat ttacaccaat attttctgcc atacttctta
    16801 cagatggaag tgattcattt attttcaatt ctcctgaagc tatagcctta gttatttctt
    16861 ctttaatttg tacataaata gatttatcac tattaaaatc taaatttaaa accataaaaa
    16921 acctccatta tgctttataa atgaaaacga ttgttatata catagtataa cataaaaaat
    16981 aataacaaac acaaaatata agtttatatt ttgtaaaaat tgtaatttaa aaaataacat
    17041 aatttttatt aaaataatac agatactatt tatagataga aataaaaatt agaaaggaga
    17101 aaaaatatgt taacatattt aatgaaaaga caaatgcata aaaaagatga tggaatgagt
    17161 aaaccaatga tgattacagg agctgtaatt acaggtgtag gagcatatgc tgtatacaaa
    17221 atggtaaaaa gccataaatc taattctcaa atggtcaata caaattatta tggaaatagc
    17281 gattatgatg attataaata tgacgaattt gattatgact gtgattgtag agacacaaaa
    17341 caagattatg aatatgaaga attaaaaaaa aaagtaaaag aatttaattc aagaagaata
    17401 aattgtgaaa attgtccaca tgtttctcaa gaagaaatga tgcaatatgt aaatagtgtt
    17461 aaagatgtaa aggaagatat tgatttacat gaagatgcta aaaaagataa tatttacaaa
    17521 gaagatgaat acaaaaaata tgaagaagaa taagtaatat tttgtataac tg
//
LOCUS       DAEPOG010000077        17070 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEPOG010000077 DAEPOG010000000
VERSION     DAEPOG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.077"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(103..789)
                     /locus_tag="KR432_002103"
     CDS             complement(103..789)
                     /locus_tag="KR432_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH0640634.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(838..1371)
                     /locus_tag="KR432_002104"
     CDS             complement(838..1371)
                     /locus_tag="KR432_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0640635.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1415..2047)
                     /locus_tag="KR432_002105"
     CDS             complement(1415..2047)
                     /locus_tag="KR432_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH0640636.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2137..3216)
                     /locus_tag="KR432_002106"
     CDS             complement(2137..3216)
                     /locus_tag="KR432_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0640637.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3349..4695)
                     /gene="pepV"
                     /locus_tag="KR432_002107"
     CDS             complement(3349..4695)
                     /gene="pepV"
                     /locus_tag="KR432_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBH0640638.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4764..6044)
                     /locus_tag="KR432_002108"
     CDS             complement(4764..6044)
                     /locus_tag="KR432_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBH0640639.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6171..6431)
                     /gene="hfq"
                     /locus_tag="KR432_002109"
     CDS             complement(6171..6431)
                     /gene="hfq"
                     /locus_tag="KR432_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH0640640.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6545..7480)
                     /gene="miaA"
                     /locus_tag="KR432_002110"
     CDS             complement(6545..7480)
                     /gene="miaA"
                     /locus_tag="KR432_002110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH0640641.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7518..9485)
                     /gene="mutL"
                     /locus_tag="KR432_002111"
     CDS             complement(7518..9485)
                     /gene="mutL"
                     /locus_tag="KR432_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH0640642.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9496..12339)
                     /gene="mutS"
                     /locus_tag="KR432_002112"
     CDS             complement(9496..12339)
                     /gene="mutS"
                     /locus_tag="KR432_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH0640643.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12502..13236)
                     /locus_tag="KR432_002113"
     CDS             complement(12502..13236)
                     /locus_tag="KR432_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640644.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13205..14218)
                     /locus_tag="KR432_002114"
     CDS             complement(13205..14218)
                     /locus_tag="KR432_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640645.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14240..15010)
                     /locus_tag="KR432_002115"
     CDS             complement(14240..15010)
                     /locus_tag="KR432_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640646.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14985..15275)
                     /locus_tag="KR432_002116"
     CDS             complement(14985..15275)
                     /locus_tag="KR432_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBH0640647.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15406..15505)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16125..16214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16267..16443
                     /locus_tag="KR432_002117"
     CDS             16267..16443
                     /locus_tag="KR432_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640648.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16558..16914)
                     /locus_tag="KR432_002118"
     CDS             complement(16558..16914)
                     /locus_tag="KR432_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640649.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         5387 a         2928 c         1800 g         6955 t
ORIGIN      
        1 attaatttga tgataaaagc tattttcctt ctaaataaaa aatttgtcta aagtttctaa
       61 aaaatttaga caaatcttag gttattattc tttaaattat ttttaaatta ttccaaatac
      121 accaaataat attggtataa atatagctgg taacaaatta gctactttta tattcgttaa
      181 tttaagtaca tttagaccaa gtcctataat taataaactt ccagctgctg tcatttctgt
      241 gactacagga tcacttaaaa atccatttaa aaatgatgct cccacacata taataccttc
      301 atacaaaaat acagatattg cagaaaacat tacacctata cctaaagaag cagtaaatat
      361 aattgaagct attccatcta taacagattt tacaaataaa gtatcatgac ttcctgtaag
      421 accactttct aatgaaccaa ctactgccat ggctcctata cagaataata aactagatga
      481 tataaaaccc tctgctatag aatctttttt atttccttta gaaaatttac tttgtaaaaa
      541 agcacccaac ttatttaccc atttgtcaat atctattaat tctcctatca gagcacctac
      601 agctacggat attattgtca ctaatgtgtt ttttccttct agtgctcctg atataccaat
      661 gtacaataca cataaggcaa ttccattcat tatagtatca cttactcttt gaggtaatcc
      721 tccttttatt ataagaccta ctatacatcc acctgcaatt gctagactat ttgctattac
      781 tccaaacatt ttttctcccc tcgattagct aattgaaatt tatcctatta ttaaaaccta
      841 agcatctata ctttgtattg gatattttaa atattccaaa aattcttcaa aaccttttct
      901 agatatattt acagttttag tatttatgtt aggatgaaaa tttaagtaat cttctttaat
      961 tacatcttta tctatgtaaa gctcaacttt tctttctgta tcatttatta atgaaaaagg
     1021 atttacactt ccaggaagaa gtttaagtac attgtatagt ctttcaggtg aagcaaacga
     1081 taatctagaa cttcctatct tatctttaag tgagtttaaa tcaacttgtt tttctccttt
     1141 aactacaata agataatact tatttccttt ggcatttctt aaaaataaat ttttacattg
     1201 acaccccttt gttattccat ctaaaatgac taattgctct gctgtatata cagcttcatg
     1261 ctctactaca tcatattcaa catttaattt atccaaaata tcatatattt tagtttcatt
     1321 catgctagat tcattttcat cttgttcttt tttattattt aaatcactca tacttttctc
     1381 cttaaacact tcatcataat atcattgtga ggtattattc tactatagct attgctctta
     1441 ctggagctcc atcagcatca ttatacttaa atggtgctgc tataaataga aatttttctg
     1501 gaacattttc taaatttgta agattttcta tgattatttt tcctttttca aataaaatat
     1561 gatgcacttc aaatactgat gtgttatatc tatccactga tagcatatct attcctatgc
     1621 cttttatatt agtattagct atatagtttg cagcttcttt tgttaatgta ggataaccta
     1681 catagtactg ctttttatcc caaaattttg accaacctga tttaaatatt ataaaatcac
     1741 tattttttat ttcatcttca tattttttta gatattctaa ttctatattc tcatttccct
     1801 tttcaagttc caatacataa gctttcccaa caaaattttc tatatccaac ttatctaggg
     1861 tctcaccttt agtatacata tgtcttggtg aatccatatg agttccattg tgagaaaata
     1921 cactaattaa tgtttcttga taaccatttt cttcaataat agctactttc ttgatatctg
     1981 gtctattagg ctctgcatat acaggcatgt cattatgtgt cacatgagtt aaatcaaaaa
     2041 ccttcatctt tatatacccc ctatgttttt attttctatt atatacctaa attcatttta
     2101 tttctatcag attttattcc attatatacc atactactat atatttttta taaacctcaa
     2161 gggatttacc gaaaaatctt ggaaagaatt atcagtaaat ataacatctt cacccaaaaa
     2221 acgtttatac cttccttctt ctttctttaa agtaattctc tgtttttgtg aatgattatt
     2281 aaaaaccatt atcttaagag gaggtttttt tctattagtc tgcactcttt ttaacatgta
     2341 ttttatgtgc atatcagctt caggaaatga atatccacaa aatattaaaa ggtcagtatc
     2401 tcttaaagtt ttttctgttt cattccaaac attacttaaa aatatgtttg acatattctt
     2461 aaaatatgtt ggtggcacta taataggttc tgttaactca ccacactcta aacattttgt
     2521 ctctgaacta ttttcaataa gcttcatgac accaccttca tgtggtgtaa gagttacact
     2581 attacaaatg gggcaataaa gccaattcaa tgacccatgt atcttatata atttgattaa
     2641 tggttctttt ggttttctcc aatttaactc ctctttaaaa tttgtaaact ccacaccata
     2701 atcaagcata attggcaaat cattcttatc tcttaatcca gtaactgtat tgtctatatg
     2761 tatatcataa tttgcactaa taaaagtagt atcaagaagc aaatcatttt ccaaaagatt
     2821 ttcaactaaa agtttatgat atttattatc tgttctaggt gtattatgaa ttgcttcagc
     2881 cattagcagt attaaatatt gtcttaaaaa acgtatacta tcacttctgt tatttagatt
     2941 ttctagtcca aaatttctaa atgcttcttt tctttgttct gctaagtcta aaagacctaa
     3001 aacttcttca aaggtaggaa aatttgcatt atctatatta tcttttacta tatcaatatt
     3061 gaacatctgt ttaaagaaaa tatacaattc tctattcatt ttggtattat aatttgtggg
     3121 atttagtttt ttaaaatatt cgctaaaaag ttcattttga ataggtagat tctctgctgc
     3181 tgaagctcct gctcctaaaa atattgctat tttcatatag ttcctcctaa tataaatatt
     3241 actcctattt ttatatccat taaatatcca tatcatttat tttatgatac aacaataaag
     3301 ctgtctttta ctagaaaaaa tcctattaca agacagcctt atattatatt aaatctttaa
     3361 taattcgtaa atagaaagag catatatttg agtagctttt ataatatcct ctactattaa
     3421 gtattcatta gcttggtgtt ctgtttctgg ttttcctgga aaaatagctc caaatgcaac
     3481 acaattatct aaagctcttg cgtatgttgc tcctccagat gaaagtggtg ttccatcaag
     3541 accagtttct tcttcatata cttttctaag tgtcttaaca agaagagtat cttctggaac
     3601 atagattgaa tccaagaaat catattcttc ataatttaaa ttgtatttat ctgtcatttt
     3661 ctttaactct tttacaaaat catccttttt gtatgttact ggaatcctta catctattcc
     3721 tgcaaattct ttttcgttat caatagttac tctacctata tttactgtta gtttacctga
     3781 tacatcatcc ttgcaattag gaattatatt gtttccatta gcatcttcac caataacttc
     3841 tgctaaaaac ttaattatat tagaatctat acctatatta ttaagagcta tacaaagtct
     3901 agccactgca tttataccag tatcacttgc tgctgaatgg acactttttc caattataca
     3961 gattttatta ccttcaacag tatactcaaa atttaattta tcaagttctt tttttaattt
     4021 atcagagtat tttccaatat agattgcttt acctggaact gcatttaaag cctttccaac
     4081 tgacaactct atatcacttt tgctatcaca agtcaaatga acttgtaaaa gacctttctc
     4141 tgcatttgtt attgggaatc tagaatctgg agtaaatcca tagtttggta tttcttcatt
     4201 attttcttta tatttattta tacatctcca aagattttct tcatcagccc caaaaatgaa
     4261 tcttatcctc ttattaaagt ctacatttaa atccataaga gctttcactg cataaattgc
     4321 tgatattgta ggtcctttat catcttgtgt tcctcttcca tacaatttac catcttctaa
     4381 aacagcttca aatggagggt aattccagct ttctaaatca ccttctggaa ccacatcaac
     4441 gtgaccaagt atacctatca ttttttcacc ttgtcctatt tctgcatatc cataatatcc
     4501 atctttgtat actgtcttaa atcctaaaga tttacataag tctaatatac ctcttaaagc
     4561 tttatctaca ttttcaccaa atgggcaatt ttcagttgct tcagatataa cactaggtat
     4621 ttttacactt tcttgtattg aagaaatcat ttcatcttgt agagaattta ctttttcttt
     4681 aatttgctct ttcatattat cacccttcaa ttttcatcat aatatactaa taaatatttt
     4741 gaccttttta aaaaaaaata tgcttatttt ttcagttttt ttataaattc aatcgctttt
     4801 aaagtcccca tttttgaatg gtcaaatgta agtccacctt gaaaataaac attataagga
     4861 ggtcttattg gagcatctgc acttaattct atagaagaac cttgtataaa tgctcctgct
     4921 gccattatga cttcactttc gtatccaggc atgtcccaag gaacaggttt tacataagaa
     4981 tcgactggtg cagcttcttg tataccttca caaaaactta ttacttcatc agcattatta
     5041 agtctaatac attgaataat atcacttctt aagtcatcat attttggtaa tacatcatat
     5101 cctaattttt caaaagctct tgagcaaaag atagctccca ttacagcctg agatactata
     5161 taaggcgcca agaaaaaacc ctgaagtaca tttctagttg ttccaaaagt aagaccacat
     5221 tcttttccta ttccaggaga agtcattcta taagatataa gctcaatcaa atcttttcta
     5281 ccagctatat atccacctgt caatgcaagc ccaccaccag gattttttat taaagagcca
     5341 gccataacat ctgctccaac atcagtaggc tctttggtat ctaaaaactc accataacaa
     5401 ttatcaacca ttactatagc ttctggttta acagacttta ttacttctat agcttctttt
     5461 atatcactaa ttgaaagtga ttttctccaa gaatatcctt ttgacctttg tatcataacg
     5521 agtttagttc tatcatttat tttgttttta attccttcta aatctaagtt tccatcctct
     5581 aaaaaatcta catcatcata tgtaacacca tactctttca atgaaccttt ttcttctctt
     5641 atgcctataa ctccctctaa agtatcatat ggtctaccag taacagataa aatttcatct
     5701 cctggtctaa ctataccttg tatacataaa gtaagtgcat gtgtaccatt tacaattatt
     5761 ggtcttacaa gagcatcttc tgtattaaat actttagaat aaatttcctc tattttttca
     5821 cgaccaatat cattatatcc atatcctgta gtccagttaa agtgcatatc acttagttta
     5881 gattcttgca tagcttttaa aactttatat tgattatatt ctcttatttc ttttatttct
     5941 tcaaacttat cttttatttc ttccattatt tcttgcgata gtttaaaagt atcatcatct
     6001 attccatagt agtcttttaa taattctttt gtctcattaa gcattttatc acctgtctct
     6061 ttattatttt atttaaacat gtaaaggaga ctattttact aaaacaaata aattatagtt
     6121 tataagttag tataagtctc ctctcaatca tcttaaatta aattaattat ctatctgttg
     6181 ttattattat tgttgttttg gttttgattt gttagattaa tatactttcc aggttgtata
     6241 gttgatacag catgtttata aatcatgttt tgttgtctat tatccccttc tattaatatt
     6301 atataactat caaacccctt tacaagccct ttaacttgca ctccattaac taaatatata
     6361 gtaacaggta tcctttcttt ccttgcatta tttaaaaaca aatcttgtaa atttaaaact
     6421 gtatttttca ttagatttgt accctcccaa gattaaattg aacttctaaa atattcctac
     6481 cttatattct atataagtta tgacaaattt tcaatattaa gttgtatact taaaaattta
     6541 tatattattt tatagaatct ttaatatata agattatttc attttttaat tcgtctatat
     6601 tttcatattg gtctaaatca aaccatcttg ctgttttata tcttttaaac catgttatct
     6661 gacgttttgc ataccttcta gaatctcttt taattatttc aatagcttct tcgtatgtat
     6721 attctccatc cagatacttc aatatctctt tataacctat accttgcatt gatatcatat
     6781 cttttttaaa tcccatactt aaaagttttt ttacctcttc aaccaatcca tttttaatca
     6841 taatatcaac tctcatattt attctttgat acaaatgttc tctatctcta tttagaacta
     6901 ttattattgg ttgatattct tcattaaatt ttaaatcact tgaaaaatca ttcattttct
     6961 taccacttag acaaacttca agagctctta tgaccctttt agtattgttt ttatggattc
     7021 tattagcaga ttcttcatca agttctttta acttattatg catgtaatct attccctttt
     7081 cagcaagctg tttttgaagt tcttctctta attcattatt ggcatcagat tgtgcaaaat
     7141 ccatattata tattaaagag tttaaataaa gtcctgtgcc tccagttact aaaacacatt
     7201 ttcccttttc atttatttca tgtatatatt tttttgctct ttgttgaaac tctgaaactg
     7261 aaaatggata gtcaggctta acttcatcta taaggtaatg cttcactcct tgcatttctt
     7321 cctctgtaac ttttgcactt ccaatatcca tatattcata tatttgcata gaatcagctg
     7381 aaattatctc tgcatctata tcttttgcta attttattga caaatctgtt tttccaacag
     7441 cagttggacc tgtaagtatt ataagtggta tttttttcat aacattcctt tccatatatt
     7501 ttaaatatcc aaatatttta cataattctt ttaaacattt tttctatttc tgtcttagat
     7561 atttcgacca ttataggtct tccatgaggg catgtaaatg gattctcaca cttttctagc
     7621 tgttccaata aactttttat ttcaatatcg tatattttat catttgcttt tatagcagac
     7681 ctacatgcca tagatgcaaa tctctctcct tttaaatcat aattacttgt aatttcttct
     7741 atattatcaa ttatttgaag tataaatttc tcagtttcag gtactccaaa tatagtagga
     7801 acacatctta ccattatatg attattgcca aaaatttcta gttcaaaacc aaatttcata
     7861 aataactcca agttattttc aatttgaagc atatctacat ttgatacttc aataaccact
     7921 gggtctaaaa gtatttgcat attgatatct tgtctataga acttctccat atatctttca
     7981 tatagtattc tttcatgtgc agcatgttgg tccaacaaat acatagaatc gtctttgctc
     8041 aaaattatat aagtattaaa tacaactcca attacagaat atccatataa ggaaaacttg
     8101 cgttttgaac tacacaagta ttcttcttca ctgtccttaa tactatctcc taagtaataa
     8161 tttccttcat ttttaattcc atcaacttga aactcttctt gtatattatc atttgcacta
     8221 tcttcactta gataattaat gttttttaca tcatcttcta cacttgcatt taggacttca
     8281 cttactgatt gaaattcatt tattggtttt tcacttgata aagtcacaac ttcaatgact
     8341 tcatccttat ctttagtaat attactattt ttaggagtag attctaataa atcattattt
     8401 cttaatttat aatctgtact tttttctcta ctgttaatag cgattctagg ttgtgttttt
     8461 ttatcactat aagtagcata ttttccaata agatttgagt gtattaattt aacctttaaa
     8521 aaatctctaa gttctatata cacttcttgt tctttttcaa atttaatttc aagtttattg
     8581 gggtgtatat ttacatctat acatgatggg tctacttcta tatttaagaa gcacactgca
     8641 tgtttaccta ttggaattat agacttataa ctttctgtta ttgcatctat gattatttta
     8701 ctttttacaa atcttttatt tatatagata tgttgtaagt ttttatttga acggtatata
     8761 ttgttattac ctatatatcc attcatttta aagtgattgc acttaaactc tacatctatt
     8821 atattttccg ttatttcttt tccatatata cttctaatag tgtttacaag ttttccatcc
     8881 cctggagtat ttaacatctg tttattgtta tttgtatatt taaattgaat gtttggatta
     8941 cctattgcaa gtttatttat caaatcactt atgtttatag tttctgcatg agtagatttt
     9001 aaaaatttct gtcttgctgg tgtattaaag aaaatatctt ttataatgat ggtagttcca
     9061 ttagtagaac ctattggttc ttttgatatt atttttccgc cttcaacata tattttagtt
     9121 cctattattt catctttagt tttagtcgtc atttctaatt ttgatacagc tgaaatagat
     9181 gccaatgctt ctcctctaaa accaagagag tataaatcat acaaatcatc aattttttta
     9241 attttactag tagcatgtct caaaaatgat ttttcaactt cacttgaagg tattcctatt
     9301 ccattatctg taatcttaat tagacttttt cctccatcaa ttatatctat ggaaatttta
     9361 tttgcacctg catcaatgga gttttcaata agttctttta ctacagaaga aggtctttct
     9421 acaacttcac ctgctgctat cttatttata gtcaaatcat ctaggatgtt aattatattt
     9481 ttcattttta cacctctaag attttatttc ttttgctttt ctttgaagat tataaagaga
     9541 gtttatagca tctaatggag tcatatttaa aatatccaga tttaaaattt cttctgatag
     9601 tatatctcta tttacactat caaaagagat ttgagttaag gctacttctt ttacagaatc
     9661 ttcttgatta attttctctt tatcatcatt ttgcttcatt aaagcaactt catcatttaa
     9721 tgctttaaat ttagaattta atttatcata atcctttttt atatgtttgt agtcttttac
     9781 tataagttca tgttctattt ttagagattc atatttattc ttaaaattac tttgatttac
     9841 cttaactaat tcttcgtcca aattactatt aattatatta ttgttttctt tatctccatt
     9901 tattgcaaca ctgttgtaaa catgattttt ttccaagtct ttaagtatat attttgctct
     9961 ttctatgact tcgtctggta atttagctaa tttagcaaca tatattccat aactcttgtc
    10021 tgctccctga ggaattattt ttcttaaaaa tattattcct tccccatctt ctttgactgc
    10081 tattgaataa tttttaacct ctttaaactc ttcttctaaa tcagtaagtt catgataatg
    10141 tgttgcaaat aatgtcttac atctaatatt tttttgaata tattccacta ttgaccatgc
    10201 taaacttatc ccatcatagg tactagttcc acgacctatt tcatctaata taactaaact
    10261 tctctctgtt gcatttttta atataaggga tacttcattc atctctacca taaaagtaga
    10321 ttgaccttga gataaatcgt cactagctcc aactcttgta aatattctat ctaatattgg
    10381 aatatcagca gactctgctg gtacaaatga gcctatatgt gccataagag ctattatagc
    10441 agtttgtctc atatatgttg atttacctga catattagga ccagttatta tgttaataat
    10501 gttttctcca cgatttaaat aagtatcatt tggaacgaaa ttctcctctc ctactatatt
    10561 ttcgacaaca ggatgtctac catttcttat atcaagttta ttattttcat ttattgctgg
    10621 ttttacatag ttatttatat gtgcaactgt tgcaagggac acaaaaacat ctatattagc
    10681 tatagtctta gccacttttt gtattctatc tatgttttta tatattgtat ctcttatctc
    10741 tacaaatatc tcgtattcta aagatttaat cttttcctct gcatgtagta tcttttcttc
    10801 tatttccttt aattctggag ttatatatct ttctgcatta ctaagtgttt gctttcttat
    10861 atatgtctca tctagctttg cctgtttaaa gttggctttt gttatttcta tataatatcc
    10921 aaaaacttta ttaaatccta tttttaatga tttaactcct gttttttctc tttccctgtt
    10981 ttctatttcc tttacaagaa aggctccatt cttagaaatc tctcttagtt ccttaagttc
    11041 atcactaaaa tctgatttaa ttatgtttcc atcttttatg gttatcgtag gttcttctag
    11101 aatagcttca tctattaaat tgtatatatc atctaattta tccatttctg aaacatactc
    11161 ttttaggatt tttgcatttg ataaatttat agtatctttt aaattaggta acttttctat
    11221 cgaatttttt aagtgaatta actcttttgg tgttactctc tcaaatgcta tctttccaca
    11281 tattctttct atatcgtata tatttttaag tatatcattt aggtcttctc ttaatataaa
    11341 gtcatctttt atttcttcta ttacatctaa tctattttca attttagatt tatttatcaa
    11401 aggttcttct acatattttc ttaaaagtct acctcccatt gctgtagaag ttttatcaag
    11461 tacatgtaat aatgaacctt ttttcttatt ccctcttata gtttgagtaa gttctaaatt
    11521 agttctagta aacatatcta aaaccatata ttctgatgaa ttgtatatat ttatattatt
    11581 aatgttagat gttatttgtt tttgagtatt gtatatataa ttcagaagaa ttgataagct
    11641 tgactttatt agacctttat catcaaactt tagtttttgt agataaacat ctggaaaata
    11701 ttcttttaat atatttatat ccaaatagtt atcactaaaa ctttcattta tgtatatatt
    11761 acttacagta gctatatctc taagcttttc tataaagtct aaatcattta taattatttc
    11821 agttggatgg atttttgcaa tttcttcaat taccttatct tcatttaaac atgttgcatt
    11881 agtttctcct gtacttatat caacataagt tagacctata ttagtaccat ccttatacaa
    11941 agataataaa taattattct ttttattttc aagtaaattt ccatctaaaa ctgtaccagg
    12001 agtgatgact cttataacct ctcttctaac aattcctttt gctgtagatg ggtcttccat
    12061 ttgttcacct attgctactt tatatccatt ttcaactaat tttgatatat atgaattggc
    12121 tgagtggaaa ggaacaccac acataggagc acgttcttct aaaccacaag cttttcctgt
    12181 caatgctatt tcaagagctt ttgaagctac taatgcatct tcaaaaaaca tttcataaaa
    12241 atctccaagt ctaaaaaaca gtatacagtc tttatatcta ttttttactt caagatactg
    12301 tttcatcata ggagttaatt tattcatatc tatttgcatt atcaaacctc cttcttttct
    12361 aattaaatat tatatcatag tagatgatta ttattaacac aatatataaa attttgacat
    12421 ataactaaaa acaatccaaa atgtatactc acattttgga ttgctaagtt taatgcttat
    12481 taaataaaaa tatatatatt tttacaataa agatattatt tcttttttaa gtttaatgat
    12541 attttcaagg ttatcttcat ttaactttct actatctata acaaattctt tctttactat
    12601 tgatggtttt tttgaaataa cataaattct gtttgatagc attatagctt cgtcaatatc
    12661 atgagttata agaagtattg ttgaatttac ttttttctta acatctaaaa gccatttttg
    12721 catggatgtt ttagttaatg aatcaagtgc cccaaaaggc tcatctaaaa gcattatgtc
    12781 attagaattt ataaaagttc tcaagaagtt agctctctgt ctcattcctc cagaaagttc
    12841 tgaaggatat ttatcttcat atccgcttaa tccaaacacc tcaaaataag gttttaccat
    12901 tgacctagct ttactctttt tttctttttt taaaattagt ggtagtgaca cattatctat
    12961 tatagtttta tatggcaaga gtaaatcttt ttgatacata taactatatt taccatttat
    13021 attcactttg ccactatcat aatctgtaag tttagttata atattaaaaa tagtactttt
    13081 tccacatcca cttggcccta atatagaaac aaactcacct tcataaacat caatacttat
    13141 atcttttaaa acttcaatat ctttataagt ttttgataca ttttctatag atacttttac
    13201 ttttttattg tggtaaaaat tcatttgtgt aagcatcact tgccttcata tccttatcta
    13261 ttaatttata ttcttttaag aatgatgtat agttgttcca tacactatct ttcatttctc
    13321 cccatcgtgg tgcatcatct ttgtacttgc ttgctaaata ttcttgactc tttaaagcta
    13381 gtttttcatc tacttcaggt gcatgtttta ctaaaatttt tgctgattct tctggatttt
    13441 ttattgcata ttcataacct tttgttgtag cttttaagaa ttttttagca agctctggat
    13501 tatctttaag cacagtttca ttagaaatta taagtggtgt ataataatcc aatctctcat
    13561 ctaaatcttt tactggtata aagtttaagt catatccaat taaatcagct ttaactgcat
    13621 cccaaccttc aaatatccat gcaaaatcta cagttttcat agctgcaaag aaatcatctt
    13681 gaccagtatt aactatcttt aacttattaa aatcagctcc atttttcttc ataactgctt
    13741 taaaaacagc ttcttctgat ggagagcccc aaccaccata agtttttcct tcaaaatctt
    13801 tagctgttgt tatattcttt tcttttggtg atgcaaaccc agaagtatta tgttgtataa
    13861 cagttgctac agcttttatt ggaagtggat cttcactagt ctttgcataa gtaacttgtt
    13921 cttgatagct tataccaaag tcagcttttc ctgttgctac taaagtttct gctccacttt
    13981 caggtggttg tactatttca acatccaaac cttcttcttt atagtaaccc ttatcaagtg
    14041 ctacaaataa tccagtatga tttgtatttg gtgtccaatc aagcaccata gttacttttt
    14101 tagaagaagt atctttatca ttgtttttgt ctttattgtt atttgaagaa catccagtta
    14161 ttgttgccaa tgtaaaaaca cttactaaac ctaatattcc taacttttta aaattcataa
    14221 ttgcctccct caaatacctt taatcaatta atctatgtct tataacaact ttttttacag
    14281 cctgtacaat tcctacaaac attaaactaa atataactac taatatagta gatgcaaata
    14341 ctttatcaag tgcatatgca ctttttgacc taagcatata aacaccaagt cctttcgttc
    14401 cacctagcca ttctgcaaca gttgctgcca ttacagcata cgttgcagat attttaagtc
    14461 cagaaaaaaa tttatccatt gccataggaa atttaaggtg aatgaatgta ttaactttat
    14521 ttgagtccat agtcttaaat aaatttaaat aatctttatc aatattctca atgccatcta
    14581 caaaacttat taatatagga aaaaaacaag taagcattac catcaatact tttggcattg
    14641 ttccaaatcc aaaccatatc ataaaaagtg gtgctataat tattgtaggt atcatctgtg
    14701 taaccaacat aataggataa agacattttt ttattattga tacaaaatcc atcaatattc
    14761 caattaccaa tgatattata attgcgacta taaaaccaag tattgcttct cctatagtaa
    14821 ccacagcatg catggataaa ttttgataat ctttaaaaaa cacattaatt atatctactg
    14881 gtgtgggtag tatatactga ggaacttcta aaactcctac catagattgc caaagaataa
    14941 gtataaacaa aaatgttgct attggatata tactatcttt gactttatct atgatttcta
    15001 agtttttcat ctatagtcag acctccagct ttttcatcta ctctagctac tgttatgact
    15061 ctttcacatc caagattaaa agggatttta tgtacctttt ttaataattc aaaaacttca
    15121 tcaaaatcac cttcaacagt agtgctcatt gctcctattt cacatttaag accacttttt
    15181 tcaatttcag caatacaagc atctactact ttatacattt cttcttcacc tttgtaaggt
    15241 cttagtggca ttactgctat gtctgcgatt accatttaaa tcactccttt aaaaataatt
    15301 aatacctact aggtatattt tgttttttat taaaaaattt tattactaaa aagatattat
    15361 atttaaataa aagaaaagtc ttatccaaaa taggataaga cttattttta tcttaactgg
    15421 tctccctaca ctggcattat ccaaacaggt tcaaagggtc gagataaaat ctcctctcag
    15481 cacattagct ccccgaaata tattatatct tttctacttg attatactac acttatcata
    15541 ttttaacaaa ttttttattt atattagata ttttatccgt tcaaaaatat ctaatataca
    15601 ttgtacatta ttacaccaaa taaattatct taaaatggta atttatcaac atcttccaaa
    15661 actagattaa ctgaattttc tagtttttat tttgctattt caagcatatt tggatgtata
    15721 tcaattccat acaaatattg ttctaaattt ctgttttcac taatctaatt tgcaatcgtt
    15781 acagattaca gattagaaat agcttttaga ctctacttat ctttatctac aaaaaatcct
    15841 tcaataaatt cagtaataat attcagtaat ctaaaaataa acctctatct ggatttttaa
    15901 gatacaagcc ttttaggaag tatcttttgt gataatataa aaattactcg tcattatcat
    15961 caaaatcctc agcaaccaac attttagata agaatcaaac tctataatat aattatttta
    16021 tccaatttat ttttgagaaa taaaatattt ttttaatttt taaatttttt aatatgcacc
    16081 ttgaaaaaag aaacgttttc ctatattatg gtaatagtag aataaaacta aataggcaaa
    16141 actagagaaa tttagtgacg caaagctata gggactaaga cttatgaaat gagttatgtc
    16201 agccagttgc caaagagata ttttcttttt gtttttatga atgtttttta gggggatata
    16261 tgatatatgt ttatggataa aatggataga tgtacacata tactaactgc atatatatgc
    16321 agttcacgcg attactgcaa ttttatagat acacaattaa atgattttat actagaatat
    16381 ggagagaatg tggtagaatc ttgtttgcat caagtgatgg tattggtaag cagatataat
    16441 taaagaaatt ttattttcta gaaaaccctt tttagtttta tctacgtttt ataattcatt
    16501 aaagaactaa tcaaaataag cttcattata tatttttcat gaagtaaaca gttgagttta
    16561 tctcatatac tgtttacccc aattacataa ttctaataat atagattcca atgatttacc
    16621 acgtttagta gacaaatatt caacttttgg aggaatagtc ggataagaaa ttctcttaat
    16681 aagtccatct ctttcaagat actttaattc ttgtgacagt atcttatgac ttatatgaac
    16741 aacatctttt tttaattgac tgtaacgtat tggctcttgt tttaaaatat gccataaaat
    16801 aattgccctc catttacttc ctatcacagt catggtaaaa ccaattgaac aattaaaatc
    16861 tttttgtctt tcttcaatat ttggaaattc ttcaaaatta ttatctatat ccatctcaat
    16921 cctctctttc aaaatagtaa gtatctaact aaaaagtgca ttcttgttta gcgtactaat
    16981 tcgtaatata atcttgatta aaaaatagct acgcacactc gtgactttag tcatgagtta
    17041 gtagctaaaa tagtcaacat atagggaaac
//
LOCUS       DAEPOG010000078        17064 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEPOG010000078 DAEPOG010000000
VERSION     DAEPOG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.078"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..251
                     /locus_tag="KR432_002119"
     CDS             <1..251
                     /locus_tag="KR432_002119"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH0640650.1"
                     /translation="NEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKEKPVCVA
                     TKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            256..1455
                     /gene="coaBC"
                     /locus_tag="KR432_002120"
     CDS             256..1455
                     /gene="coaBC"
                     /locus_tag="KR432_002120"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH0640651.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            1694..4219
                     /gene="priA"
                     /locus_tag="KR432_002121"
     CDS             1694..4219
                     /gene="priA"
                     /locus_tag="KR432_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH0640652.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            4237..4677
                     /gene="def"
                     /locus_tag="KR432_002122"
     CDS             4237..4677
                     /gene="def"
                     /locus_tag="KR432_002122"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0640653.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            4692..5621
                     /locus_tag="KR432_002123"
     CDS             4692..5621
                     /locus_tag="KR432_002123"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH0640654.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            5631..6383
                     /locus_tag="KR432_002124"
     CDS             5631..6383
                     /locus_tag="KR432_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBH0640655.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            6452..7153
                     /locus_tag="KR432_002125"
     CDS             6452..7153
                     /locus_tag="KR432_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBH0640656.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            7215..8540
                     /gene="rsmB"
                     /locus_tag="KR432_002126"
     CDS             7215..8540
                     /gene="rsmB"
                     /locus_tag="KR432_002126"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH0640657.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            8548..9579
                     /gene="rlmN"
                     /locus_tag="KR432_002127"
     CDS             8548..9579
                     /gene="rlmN"
                     /locus_tag="KR432_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0640658.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            9599..10351
                     /locus_tag="KR432_002128"
     CDS             9599..10351
                     /locus_tag="KR432_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBH0640659.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            10368..12383
                     /gene="pknB"
                     /locus_tag="KR432_002129"
     CDS             10368..12383
                     /gene="pknB"
                     /locus_tag="KR432_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBH0640660.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            12491..13228
                     /locus_tag="KR432_002130"
     CDS             12491..13228
                     /locus_tag="KR432_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0640661.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            13326..14210
                     /gene="rsgA"
                     /locus_tag="KR432_002131"
     CDS             13326..14210
                     /gene="rsgA"
                     /locus_tag="KR432_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0640662.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            14215..14886
                     /locus_tag="KR432_002132"
     CDS             14215..14886
                     /locus_tag="KR432_002132"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0640663.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            14895..15536
                     /locus_tag="KR432_002133"
     CDS             14895..15536
                     /locus_tag="KR432_002133"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBH0640664.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            15667..16296
                     /locus_tag="KR432_002134"
     CDS             15667..16296
                     /locus_tag="KR432_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0640665.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            16351..17007
                     /locus_tag="KR432_002135"
     CDS             16351..17007
                     /locus_tag="KR432_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0640666.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6914 a         1709 c         3277 g         5164 t
ORIGIN      
        1 tcaatgaggt actagaaaaa atagacaaca gatactattt agtagggact gtttcaaaga
       61 gagcaagaaa attaattgat ggagaagaac catatgtttc aaataaaact aaggaaaagc
      121 ctgtatgtgt agcaactaaa gaagttgcaa gtggaaaaat aacatataga ttattgacag
      181 aagaggaaat agagatagaa gaagctagac atcatgctga gcaacaccaa caaatttcag
      241 aagaggagta aggtcatgct aaaagataaa actgttgtaa ttggcataag tggcggtata
      301 gctgcatata aggcttgtga tgttgtaagt aagttaaaaa aactaaatgc aaatatccat
      361 gttataatga caaaatcagc aacagaattt gttaaaccac ttgcatttca atcgctgagt
      421 caaaattatg tagtagaaga tatgtttgaa gaaccaaaaa cttgggaagt tgaacatata
      481 tcattagcaa aaaaagcaga tttattctta atagttccag caagtgcaaa tgttataggc
      541 aagattgcaa atggtatagc tgacgatatg ttgacaacta ctgttatggc aaccaaagca
      601 caggttctta tagcaccagc tatgaatact aatatgtatg aaaatccaat tgttcaaaaa
      661 aatatacaaa ctttaaagag ctttggatat aaatttattg aaccagaaag tgggaggctt
      721 gcatgtggag atattggaac aggcaaatta gctaatccag acataatagt agaggaagtt
      781 ttgactaatc ttactaaaga gcaagattta aaaggtcaaa gtatgatagt tacagctggt
      841 ccaactgtag agagtataga cccagtaaga tatttaacta ataggtcttc tggtaaaatg
      901 ggatattcta tagcaagaga agctatagct agaggtgctg atgtaacatt aatatcaggt
      961 cctacaaatc tttctattcc acaaaatctt aaaaaatttg ttcaaataga gtcagcacaa
     1021 gatttgtatg aagcagtagt atcaaattta agtgaaaata aaatagtaat tcaaagtgca
     1081 gcagttgcag attataaacc aaaaacttat tctgacaaaa aaattaaaaa gaagaatgat
     1141 gatttatcaa tagaactaag tagaaattat gatattgctt atgaaatagg aaaaataaaa
     1201 gaagataaga tactaattgg atttgcagct gaaactaatg accttataga acatgccaaa
     1261 ggaaaaataa aaaagaaaaa tttagatttt attgtggcaa atgatttaac taaagaaggt
     1321 gctggtttta gagctgatac taatattgta aaaataatag atgtagaggg aaatattact
     1381 gagtatccta agatgaaaaa agaagaagta gccaatgtta ttttagataa agttaaagcg
     1441 ttgcttgaga aataagacct tttttaaata tgatatttgt gtattttatc acagctaaag
     1501 tggctgaaag ctgcaattta attttagcga gtaaaaactc gcaaatgtca gttaaattgt
     1561 agttttcagc atttattttg tcataaagta taaaatataa ttaatagtta attattaaaa
     1621 taaagttaat attaataact tgaaattgct tataaattct gagattttaa tatcttgtaa
     1681 agtatattaa aatatgaaca atatattaaa tttaagtaga ggttttataa tgaaaaaata
     1741 tgcaaaggta atagttagaa gtaatactat atatacagat aatttattta catatcaaat
     1801 tcctgtattt ttatctgatg taataaaaat cgggcacaga atattagtac catttggaaa
     1861 aggtaataaa ccaacagaag cctttgtatt tcaatttaca gatagcttag atgaaaaaat
     1921 aaaaataaaa gaaattattg atatattaga tgaaaatcca atatttagaa aagaagattt
     1981 agagttggtt tattggatga aaaatagata tttgtgtact tatatagaat gtataaattt
     2041 gatatatcca aagggataca aattaaataa ttataaagta gtcctattag gtgaaagttt
     2101 aagtggatta aatgagattg aattgaagga aaaaatatca actttaagtg ataaaaatag
     2161 agaaatagta gagaaagtga ttgatagtaa aggtaaaatt aaggttgata agttaaaata
     2221 tataccaaac ttaaatagtt ctttgtacac aatgaataaa aatggaatta taaaattatg
     2281 ttgggaatat aaaaatcaca aaaatgaaaa gaaagtatgt tatatatctt tgagtttaga
     2341 aagtgataag attgacgatt atatagagca aaataaaata aatgtgggaa gtaaacaaaa
     2401 agagatatta agttttctta aaaataatga aaatgtagag ataaatgatt tattggattt
     2461 attaaatgct tcaaaacaaa gtataaattc tctaagtaag aaaaagctaa taaccctaga
     2521 atttaaggat tattatagag agccaaaaag tatttacaaa tccgtactaa aaagtataaa
     2581 attaaacaat gaacaacaag aagcagttga tgagataaag tcgaatatgt ttgtagatga
     2641 taaaaaacca tacttaattc atggagtaac tggaagtgga aaaactgaag tttacatgga
     2701 aataatagaa tttgctctaa atcaaggctt agacagtata ttcttagtac ctgagatagc
     2761 attaactcct caaacaatag ataggctaaa gagtaggttt ggagatttag ttggagtgtt
     2821 ccacagtaaa ctatctgaag gagaaaaaca tgatgtttac aaagctgtca aagcaggtaa
     2881 agtaagagtg ttaattggag ctaggtcagc tttatttgca ccttttaatt ccttaggctt
     2941 aataataatt gatgaatgtc atgagtcatc atataagtct gagaagaatc caaaatttaa
     3001 tgctatagaa gttgctagat ttatggcatt aaaaaataat ataactctta ttttaggttc
     3061 agcaacgccc tcaattgaag agtattatag ggcaaaatct ggagaataca aacttataaa
     3121 tataaaaagt agagcaaatg ataagcctct tccaaacatt gaagtggtag acatgaaaga
     3181 tgaattagat aaaggaaata gaagtatatt tagtttgaaa ttacaaaaag aaatacgtta
     3241 tgcgattgaa gaaaataatc aagtgatact atttttaaac agaagaggtt atgcaaattt
     3301 tgtatcttgt agaaagtgtg gatatgtgtt tcaatgtgaa aattgtgata tatctttaac
     3361 ttatcataag aaaagcaata ctggaagatg ccactattgt ggatatgaga aagaaattcc
     3421 aaaagagtgt cctgaatgta aaagtactta tgttaagcca tttggagttg gaactcaaaa
     3481 aattgaagag gaattaaaat atatttttcc agatataaaa actttgagaa tggataaaga
     3541 tacaacttca aaaaaaggtg ctttagatga gatactaaat aaatttaaag ataaagaagc
     3601 agatgtactt attggaacac agatgttaag taaagggctg gattttgaga atgtaacttt
     3661 agtaggtatt ttgtctgcag acatgatatt aaattttcca gactttaaaa gttttgaaac
     3721 tacatttcaa ttgataactc aagtctctgg aagagcaggg agagctgata aagagggaaa
     3781 agtagtactt cagacttatg acactgaaca ctatgctatt aagcatgcta ttgaatatga
     3841 ttatgagggt ttttatgaag atgagataaa aataagaaaa gcatttggat attctccttt
     3901 taataatatg ttaagtgtag ttgttagtgg tgaagatgaa agattagtga taaaaaatat
     3961 aaaaaatatg catgcatcat tgatttattt attagaaaaa aggggtataa atgatttagg
     4021 atttatatta ggtccaaatc catgttcaat atcaaaaata aatcaaaatt atagatggca
     4081 aatactcttt aaagatgaaa atattgaaat taatctctta aagggtataa taaaatatat
     4141 atgtataaca aaacgagatt tgatatttga taagaatatc aatgtatcta tagatattaa
     4201 tccaaacagt gtattataaa tttaggagga attataatgg ctttaagaca aatagttcaa
     4261 ataggagaac cagtattaag aaaaaaatca aaaaaggttg aaaaaattga tgaaaagata
     4321 atacaattat tagatgatat ggcagaaact atgtatgatg cagatggtgt tggactggct
     4381 gcacctcaag ttggaatatt aaaaagagta gtagtaatag atattggaga agagcttata
     4441 gaacttataa atcctgaaat aatagaaaca tctggggagc aaatagatga ggaaggatgt
     4501 ttaagtgttg ttggagaagc tggaaatgtt agaagaccaa attatgtaaa ggtcagagct
     4561 ttaaatagaa atggcgaaac aatagagtta gagggagaag aacttttagc tagagcattt
     4621 tgtcatgaga tagaccactt agatggaatt ttatttgttg acaaaataga aaagtagtag
     4681 gagttgattt gatgaaaata gtatttatgg gaactcctga tatagcagtt ccatgtttac
     4741 aaaaaataat agatgagaag tatgaaatat tgggtgttgt aactcaacca gataaaccaa
     4801 aaggaagagg taaaaaacta ggtatgagtc cagtaaaaga actggcaatt gaaaataata
     4861 tacctgtata tcaaccagta aaggctagag ataaagaatt tatagataaa attaaatctt
     4921 taaatccaga tgtaatagta gttgtagctt ttggacagat acttccaaaa gaaatattag
     4981 agattcctaa acttggatgt ataaatgttc atgtttcttt acttccaaaa tatagaggtg
     5041 cggcacctat aaattgggta ataataaatg gtgaagaaaa gactggtgtt acaactatgt
     5101 atatggatga agggttagat actggagata tgatactaaa gacagaagta aatcttgatg
     5161 aaaatataac agcaggagaa cttcatgata aaatgatgaa tataggagca gaaactttaa
     5221 aagaaacatt aagattaatt gaagaaggaa atgctccaag agaagtacaa aatcatgaag
     5281 aattttccta tgcgccaata atgaataaat cattaggtaa catagatttt tcaaaaagtg
     5341 ccagagaaat acacaattta gttagaggag taaatccatg gccaagtgct tatactatct
     5401 ataatgatgt cataatgaaa gtttggaaaa ctaaagtgtt agatgaaaaa agcacaaaag
     5461 atgtaggtac aataattgat gtaagcaaag atgggataaa agtaagtact atagataatg
     5521 tacttttaat tgaagaaatt cagatgccga ataaaaagag aatgttggtt ggtgaatata
     5581 taaaagggaa tactatagaa actggtttag tattgggtta ggtgaaaagt atgacagata
     5641 taaagaagta tttaacaagt gttgggattt tgattttaat attaggaatt ttgataggag
     5701 ttgttaattt ttttataata agtttaactc aaatattctc cttgatgata aatattataa
     5761 taatatcact aatacttata atactatgtt caacagcagt tacttataga gtaattaaag
     5821 gaaagtatgt taactcaaat ataatgaaaa taaattttaa tattgtcagt ggattatttc
     5881 ctattatatc tttcattgca tcatcatttg gtatgtcaaa aagtgatatt agaagaatat
     5941 atataaagtt aaataatgaa tatatataca gtaataaata taattttaat ccagaggata
     6001 taataatttt aatacctcat tgtattcaag agaatagttg taaattaaaa gttactaatg
     6061 atatagataa ttgtaaagaa tgtggaagat gcaacgtagg agaattaatt aagctaaagg
     6121 aaaaaactaa tgtaaaaata tttgtagcta caggaggaac cctagctaga aaaattataa
     6181 tggataataa acctaaagct gttgtagctg ttgcatgtga aagagatttg acatctggaa
     6241 tacaagatat taagaaaatt cctgttttag gtgtatttaa caaaagacct aatggacctt
     6301 gtgtaaatac taatgtagac atgatggata ttgaaaaagc tatagacttt ttaacaggaa
     6361 aaaatatatt aatttgtaat taaactgttt ttaagttaag tgaatattaa gaggatgtat
     6421 aataaaaata tattttagga gagtgttatt tatgtaccct gtatatggcg gtttttgggg
     6481 atttgaccca acaatggttg tattaattcc agctatatta cttactatat atgcacaatt
     6541 taaagtgagt tcaacaacaa ataagtattt aagagtaaat acacgtcgag gatatactgg
     6601 agagcaaaca gcaagaagag tacttgattc aaatggtttg tatgatgtaa agatagaaat
     6661 ggttagaggg catctaagtg accattatga cccaagaaga aaggctgtaa ggttgtcaga
     6721 agatgtatat tatggaacat ctataacttc tgttgcagta gctgcacatg agtgtggtca
     6781 tgccatacaa catgctaaag gttatgcgcc tcttcaaata agaagtagtt tagtaccagt
     6841 agtgaatttt gcttcaagta tatcttggtt tttaatattt ttaggattta ttatggctgg
     6901 tccattttta aaaattggta tactattgtt ttcggcttca gtgttattcc aaataataac
     6961 attgcctgta gaatttaatg cttcaagtag agcaatcgtt caacttggga atttaggtat
     7021 tatagatgaa agtgagtcaa gacaaagtag aagagtactt tcagcagcag ctttaacata
     7081 tgttgcagca gcattggttt caatacttca attacttaga ttgttactaa tagctcaaag
     7141 aagaaatgac taaaagtaaa gtgggttgtt tagaaacaac ccttttttat gtttaataaa
     7201 agggagaaat ttatatggat gcaagagaag ttggatttaa agtattatgt gatatagaaa
     7261 aaaatgacaa ttattcaaat attgcaataa ataagcattt taaaaattta gaaataagtg
     7321 atatggatag aggacttgca acagagttaa tttatggagt aatagaaaat aaatattatt
     7381 tagattatat aatcaataaa ctttcaaaaa taaaggtaaa aaaaatgtca acttatgtta
     7441 aaatcttttt aagaatggga acatatcaga ttttattttt agatagtata tcagattatg
     7501 cagcagttaa tgaaactgta aaattatcta agaaatatga taaaaaatct tctggtttca
     7561 taaatgctat tcttagaaat gaaataagag ctaaagagac tataatggac ataactgagg
     7621 aagatagtgt aaaatactta tctataaagt attcctataa ctcttggatt ataaaaaact
     7681 ggatagataa atttggacaa gaatttgctg aagatttgtt ggaagcaaat aatgaaaagc
     7741 ctagtatcta tataagaacg aatacactta aaataagtag agaagaactt attgaaaaat
     7801 taagtgaaat gggtattgta tgtttaaaag ttcctatggt agaagaagct attaaagtcg
     7861 aaaaaatgaa aaatatagaa aaaaatgaat tatttaaggc tggtttattt acaattcaag
     7921 atataagttc tatgatagta gggaaagtaa ttaatccaaa agaaggttca tttgttttgg
     7981 atgtatgtag tgctcctgga gggaaatcta ctcacttagc tactttaatg aacaatacgg
     8041 gtcaagtagt agctagagat atttttgagc ataaactaaa gcttataaac tctactgtaa
     8101 atagattggg gcttaaaaat gtgtttgtag aaagatttga tgcttctgag attgatgaaa
     8161 atagtataag taaatttgat tatgttttag cagatgttcc ttgctctggt tttggtataa
     8221 taagacgtaa accagaaatt aagtataaga aagaagaaga gttaaaagac atcacttcta
     8281 tacaaaagaa aattttagaa aatgcatcta aatatgtcaa agttggtgga actttagtat
     8341 atagtacatg tacaattcaa gatgtggaga atataaatat agttacttct tttgttgaag
     8401 aaaatcacaa ctttgaattt gttccaattg atacagtaaa tgtagactta gacaatcaag
     8461 ataaaggata tttaaagata tatcctaata ttcatggtat agatggtttt ttcatagcaa
     8521 aattaaagag aataaggtag tgagagaatg gaaaagaaaa agatagtatt aaaaaatttt
     8581 actgaagatg aacttaaaga atttatgaaa actatagatg aaaagccctt tagaggaagt
     8641 caaatattct cttggatata taagggtgca aaaacttttg atgatatgaa caatatacca
     8701 aaaagtttga gaaataaatt agaagaggtt tcatgtatag ggaatataga tatagaatta
     8761 aagttagaat ctaaggtaga taatacaaaa aaatatttgt ttttattaga tgatggaaat
     8821 ataatagaaa ctgtgatgat ggattatgat agtagagtta ctgtctgtgt ttctaaccaa
     8881 gtaggttgta ggatgggatg taatttttgt gcatctacaa tggatggatt aattagaaac
     8941 ttagagccat gggaaatttt agaccaagtt attaaaattc aagaagatac tggtaagagg
     9001 gtatcaaatc ttgtattgat ggggagtggt gagccacttg ataactttga aaatacaaaa
     9061 caattcttaa aaataattaa cgaaaaaaat ggtcttaata tagggtatag acatataact
     9121 ctttcaactt gtggaatagt acctaaaatg tatgagttgg cagacttaga aatagctata
     9181 aatttagctt tatcacttca ttcaccatat gatgaagaac gaagaaaaat tatgcctgta
     9241 gcaaatgctt attctattga agaaatattg aatgcatgta gatattatat aaaaaagaca
     9301 aatagaaggg ttacatttga atattctctt ataaaaggag taaatgactc agaaaaagaa
     9361 gcaaaggcat tagctaagct cctaaaaggt atgctatgtc atgtaaattt aattccaata
     9421 aataaagttg aagaaagaga atacgaaaag ccagataaag catttattta taaatttagg
     9481 gatagtttgg agaagaacaa tatacctgcc acagttagga tgtctatggg ttctgatatt
     9541 agtggagctt gtggtcaatt gagaagaaaa tacaagtaat ttgtaaggag gaagtcgtat
     9601 ggtttatagt tgtgcctccc atataggaaa aataagaaaa aacaatgaag actattgtga
     9661 gggagaagtt atagatacag aacatggtcc aataggaata tttgccatag ctgatggaat
     9721 gggtggacat aaaaaaggtg aagtcgccag taaattagca gtagaaaata taattgattt
     9781 tctaaaggaa aacttattac aacatgataa tgtaaaaata gactatatag atgatatatt
     9841 aaaacaggct tataataatg taaactcaat tgtgcacaaa aaatctatgg aagatataga
     9901 atttgaagga atgggaacta cattagttac ggctatagtg tataataatg tcttatatgt
     9961 ggcaaatgtt ggagatagca gatgttattt gttaacagat gaaaaatttg ataaaataac
    10021 tatagaccat tccgtggttg aagaacttat gatggcccat gtcattacag aagaagaggc
    10081 aagaagacat ccacagagaa atagaataac tagagcaatt ggaacagatg atatggttgt
    10141 tgtagatatt tttaagaaag aaatcaaaaa aagtgatata atacttcttg cgacagatgg
    10201 attaactggg tttattgatg atgaagatat aagagattta atattagatt atgaatatga
    10261 gagaactagt aatataagtg aagaattgat aagtatggcc aatgatgttt ctggtaaaga
    10321 taatgtttcg gttatagtga taaaagtata attaggacgg tgatattgtg ggagatacaa
    10381 ttttaggaaa tcgatatgaa atcatcagga agattggtga tggaggaatg gcctttgtat
    10441 atgaggctaa agacagatta ttaaacagga ctgttgcact taaagtactt agacctgaat
    10501 ttgtagatga tgatgaattt ctaacaaagt ttaaaagaga ggcagaagca gtagcaagtc
    10561 tttcacatcc aaatatagta aatgtttatg atgttggtga agatggaaaa gttcactaca
    10621 ttgtaatgga gtttgtagat gggaagaatc taaaagagat tatacaagat gaaggtatat
    10681 tagatgaata tactgcatta gatataacaa aacaaatagc tatggcactt agtgcagcgc
    10741 acaaaaaggg gattatacat agagatataa aacctcataa tattctcata tcaaatgaag
    10801 gaagagtagt aaaagtggct gattttggta tagccaaagc tgtttcaaat tcaacaatga
    10861 ctaatattgg tagtataata ggttcagtgc attatttttc accagagcaa gctaaaggta
    10921 aatttgtaac taataatgct gacctgtatt ctctaggtat agttttatat gaaatgttga
    10981 taggaaaagt tccatttagg ggagatagtc caatttctat agcacttcaa cacattaatg
    11041 atgatataga ctttacatca gaagaaaaag ttaggattcc tcaaagtgta agaactacaa
    11101 taaaaaagct tacagaaaaa tctagcgcag atagatatca aactgctgaa gaattgatag
    11161 aagatataga gtatatagaa aaaaatattg atttagattt tataaaagaa tatgatgatt
    11221 ttgcaactaa gaaaattgat gaaaaagaaa ttaataaagt tgtaaatcca acacttgcaa
    11281 aaccagctcc tgaaaaagtg gttaagcctg ttgaagtagc tgatttggat gatgatgaag
    11341 attactatga tgatttttat gaagaagatg aagaagaaga agaagaagag gaagagataa
    11401 tgagagctaa aaagaatcag agacctaaga gtactccaag taagagaact aagaaaaaga
    11461 agaaaaaaca ggaaagccca aaatctagaa gaagattaaa agtaattgcg gctgttttga
    11521 tactgatatt gtgtgctcaa gtattcttag catataagtt tttatttgct ggagggtttg
    11581 gcaataaatc tttgactgtt cctaatcttg taaatatgac attagaagaa gctcaaagtg
    11641 ctgttgaaaa agaaggatta tacctgagtg taaaaagtga agagtataat agtgaagttg
    11701 atgaaaattg tataatatca cagacacctg aaggtggaag tacaaatgtc aaaaagggtg
    11761 atactataaa tgttgtcgta agtaaaggtt caagtcaggc atctgttcct aatgtggtgg
    11821 gacttacttt gagtaatgca aaacaactta ttgaagaaaa taacttaaaa gtgggaactg
    11881 ttaagtatga atatagctca atatataaag aaggtacagt tttaagtcag agtccaggtg
    11941 ctggctcatc aagagctcag gaaggtgatg aggtaaacct atatgtaagc aaaggttcag
    12001 aaaaatcaaa tacacagact ccaacagtac cagataaaaa accaacaaca ccagagaata
    12061 atacaccaac agaacctggt tcaaactcag ggaacagtgg tggaaattca ggaagttcaa
    12121 actcaggaaa tagtggagga aactcaggca ataatggagg taactcagga ggttcaaact
    12181 caggaaatag tggaggaagc tcaggaggct caaactcagg agataatgga ggaagttcag
    12241 gaggttcaaa ctcaggagat aatggaggaa gttcaggagg ttcaaactca ggagacaatg
    12301 gagatagttc aggaggctca aactcaggag ataatggagg aacttcatca ggaactggcg
    12361 caaacattgg taaaacagaa taaatattaa taaaataaga gctgtccttt tatagaaaat
    12421 attttcttta aagggcagtt tgttttgtat aaaatactga attggttata atataaggag
    12481 aaaagtattt atgaagaaga aaaccgtttt aattacagga ggagctagag ggattggtaa
    12541 agctatgtct aaagcttttg caaaagaagg ttacaatgta ttagtaaatt ttaataagtc
    12601 agaaaatgaa gctaaagagt tgtatactat attaaatgaa aaaaattttt ctgtaaaact
    12661 ttttaaggct gatatttcaa atagagaaga tgtagagaat atggtagatt attgtataaa
    12721 agaatttgga ggattggatg tacttgtaaa caatgctggt gtaagtcaag ataagttgtt
    12781 tacagatata accgatgaag attgggataa tatgatgaat attaatttaa aaggaagctt
    12841 ttattgttcg caagttgctt taaaatacat gatatcagaa aaaaaaggaa atataattaa
    12901 tatatcttca atctggggta tatcaggtgc gtcttgtgaa gtgcattatt ctataacaaa
    12961 agcaggtata ataggtatga caaaggcatt agctaaagaa gtaggaccat caaatattag
    13021 agtaaatagc atagctccag gtgtcattaa tactgacatg ttatctggat ataatgagga
    13081 agatatagac gctcttgtgg aagaaacccc tctaatgagg ctaggaactc cagaagatat
    13141 agcaaattgt gctattttct tggcatcaga caaatcaaat tttatcactg gtcaggtaat
    13201 tagtccaaac ggaggatttg ttatttaaaa aaaatgtggt attatataaa aataggaaag
    13261 aataacacga tatactattc ataaacagta tgtgtttata taaattttat aaagggaggc
    13321 atttgatgct agaaggaaaa attataaagg ggattagtgg attttattat gtagatactt
    13381 ataatggtat ttatgaatgt aaggctagag ggatatttag aaagcagaaa ataacacctt
    13441 tggttggtga tagagtcaaa ataagcattg ttgatgaaga tgaaaaaaag ggtatcttag
    13501 aagaaatcga cagtagagat acagaactta taagacctcc tattgcaaat gtagataaag
    13561 cattaatagt attcgcaata aaaaatccta aaccaaactt atctctttta gatagattta
    13621 tagttttggc agaaaaagaa aatttagaaa ctgtaataat ccttacaaaa gcagatttag
    13681 atgataatga tacattagaa actgttaaaa atatatatga acttagtggc tataaagtca
    13741 ttcctgtaag caatattact aagttaaata tagataaagt aaaagaagaa ttaaaagaaa
    13801 atgtagtagt gtttgcagga ccatctggag ttggaaaatc aagcttatta aatgaaatag
    13861 atgaaaattt taaacttcaa acaggagtgg taagtgataa aataaaaagg ggaaaacata
    13921 ctactcgtca cgctgaactt ttaaaattgg aatttggtgg aatggttgca gatacaccag
    13981 gttttagttc ccttgcactt gaagatatag aggaagttga acttaaagat tattttatag
    14041 aatttgataa atttaatgat tgtaagtttg gttcaaagtg tattcatgaa aatgaaccta
    14101 attgtgctat aaaagaagct gttacaaatg gagaaatatc taaagaaaga tatgatagtt
    14161 atatacagct gctacatgaa ataagacaaa ataatagtag gaggtattag aaaaatgatt
    14221 aaattagcac catcaatatt atcagcagat tttgcaaaat tattagaaga tgtaagaaaa
    14281 gtagaaagtg caggatgtga atatcttcat atcgatgtaa tggatggaca ctttgtacca
    14341 aacattacat taggaccact tgtagtcaaa agtttaaaga aagaaaatat aaatatggta
    14401 tttgatgctc atcttatgat agaaaatcca gaccagtata ttgaagaatt tgtaaaagca
    14461 ggatgtgata taataactgt tcaccaggag gcttgtgttc atctacatag aactatacaa
    14521 aatataaaat cacatggaat aaaagctggt gtagtactta atccagctac accagtagat
    14581 acgattaaac atgtactacc tgatttagat atggtattat taatgtctgt taaccctgga
    14641 tttggaggac aatcatttat tccttgtgtt ttagataaga ttaaagagtt aaaagctatt
    14701 atagatagtc aaggattaaa cattgatata gaagttgatg gtggtataag ccctaaaaac
    14761 gtagccgaag ttgtacaagc aggagctaat gtaatagtag caggttctgc aatatttgga
    14821 agtgatgaca ttcaagaaac agttaattta tttagaaaga atgcttctct tgaagaatta
    14881 gtttaaattt aataatgaaa atctgtattg ttttaaatgg cgaaattgaa gattacgaag
    14941 ttacaagaga tattataatt aaggaatgtt atgactgcat aatatgtgca gatggagggg
    15001 caaatcacac ttataaaatg gaaataatgc cagattatat attaggagat ttagattcag
    15061 tagaagaaga aaagataaac ttctataaaa ataaaggtgt taaatttgaa aaatttccat
    15121 ctaaaaaaga tgagactgat acagagcttt gtcttttttt agctaagaca cttaaagcaa
    15181 atcatattga tttttttggt gctttagggg gaaggattga ccacactctt gcaaatataa
    15241 agctattata ttatttaaaa gaagatggta tatattctag gatactttct gataaagaag
    15301 agatgtatat agttgaaaat gaggaaattt cattgtatgg aaacccaggg gacactgttt
    15361 ctgtaacagc tataaaaggt gatgctaagg gtgtaacact tactggctta gaataccctc
    15421 tcgataatta ttatatgaaa tactctgtac ctattggaat atcaaatgtt atgttgagta
    15481 attcttgtaa gattaaagta gagcaaggct gtgtacttgt agttagaaat ttataaaatt
    15541 tgattttgac aattggtttt atattgtaga gcagatacaa tcaagtaaaa tatatttatg
    15601 attgtatctg ctttttagta tataattata aatatataat aaactttgac ataaggggga
    15661 ttttttgtgg tagatagtat tatttttgat ttagatggaa cattgtggga ttcaactgaa
    15721 gaagtatgca aagtctggca ggatgtttta agtaaacata aggaaataga attaagtgtc
    15781 acaaaagatt tatttagaag tcttatgggg ctttcttttg aggaaatagc aaatcgttta
    15841 tttccaaatt taagtgaaga agaaagaatg aatttattga atgaatgttc tttgaaagaa
    15901 tgtgactatt tatctgaaaa tggagctaaa ttatttgatg atatagaaga tactcttaaa
    15961 ttactatcta aaaaatataa attatttata gtaagtaatt gtcaagctgg atatatagaa
    16021 tcttttttaa aagcacataa actagaacag tattttatag attttgaatg tcctggtaat
    16081 actggtttgc ataaaggaga aaataataaa cttataattg aaagaaataa attaataaat
    16141 ccaatctatg ttggtgatac gcaaggtgat gcaaatagtg ctaagtttgt aggtatacct
    16201 tttatatatg ccaaatatgg gtttgggaat gtagatgtat atgattattt tatagattca
    16261 tttaaagatt tgttagaaca tgatatatta aaataagatg agtttaaaaa ttatattaaa
    16321 ataataaaaa agttatgggg ggatttaaaa atggaattac aagatactat ttttaaaaga
    16381 caaagtgtaa gaaagtttaa aaatcaagat gtatcagatg aagatatttt aaaaatgata
    16441 aaagcagcag gtgcagcacc atcaggcaag aatattcaaa actggcattt tgttgttata
    16501 aaacgccgtg atttaatgga aaagatagca gatgtgataa ctaaaaagca acaagaaata
    16561 cttgtggaaa tggataaagt atcagtggat aaggctaata gatttagaaa atttgttaaa
    16621 aactttacac tattttactt aaaagctcca gttttagtat tagtttttac aaaggtatat
    16681 aatccatcag gatattatga attagagctt atagatgcac ctaaggagac tatagataaa
    16741 ttatttataa gaaatccagg tatgcagagt ctaggtgctt caattgaaaa ctttacacta
    16801 tcagcaattg aacttggata tggttcttgt tggttgacaa gtcaaaacta tgcagcagat
    16861 gagatagaag ctgttttaga agcagaaact ggttttgaaa agggagagta ttttttaggt
    16921 gcaatgttag ctcttggagt gccagaggac aatttgaaga gtccatctaa aaaaccagta
    16981 gaagaaatat gtacatttat aaaataaaag tcaaggacat tttaaaatca aagtaaatta
    17041 aaaacaagat aaattaaaaa taaa
//
LOCUS       DAEPOG010000079        16751 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEPOG010000079 DAEPOG010000000
VERSION     DAEPOG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.079"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(330..1721)
                     /locus_tag="KR432_002136"
     CDS             complement(330..1721)
                     /locus_tag="KR432_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0640667.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1732..2952)
                     /locus_tag="KR432_002137"
     CDS             complement(1732..2952)
                     /locus_tag="KR432_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH0640668.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2966..3754)
                     /locus_tag="KR432_002138"
     CDS             complement(2966..3754)
                     /locus_tag="KR432_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0640669.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3771..5045)
                     /gene="larA"
                     /locus_tag="KR432_002139"
     CDS             complement(3771..5045)
                     /gene="larA"
                     /locus_tag="KR432_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBH0640670.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5163..5876)
                     /locus_tag="KR432_002140"
     CDS             complement(5163..5876)
                     /locus_tag="KR432_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0640671.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            6001..7260
                     /locus_tag="KR432_002141"
     CDS             6001..7260
                     /locus_tag="KR432_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBH0640672.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7528..8094
                     /locus_tag="KR432_002142"
     CDS             7528..8094
                     /locus_tag="KR432_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0640673.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8295..9500)
                     /locus_tag="KR432_002143"
     CDS             complement(8295..9500)
                     /locus_tag="KR432_002143"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0640674.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9603..9709)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9801..10169)
                     /locus_tag="KR432_002144"
     CDS             complement(9801..10169)
                     /locus_tag="KR432_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBH0640675.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10221..11498)
                     /gene="obgE"
                     /locus_tag="KR432_002145"
     CDS             complement(10221..11498)
                     /gene="obgE"
                     /locus_tag="KR432_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBH0640676.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11609..11899)
                     /gene="rpmA"
                     /locus_tag="KR432_002146"
     CDS             complement(11609..11899)
                     /gene="rpmA"
                     /locus_tag="KR432_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH0640677.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11913..12230)
                     /locus_tag="KR432_002147"
     CDS             complement(11913..12230)
                     /locus_tag="KR432_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBH0640678.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12245..12556)
                     /gene="rplU"
                     /locus_tag="KR432_002148"
     CDS             complement(12245..12556)
                     /gene="rplU"
                     /locus_tag="KR432_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH0640679.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12657..14078)
                     /locus_tag="KR432_002149"
     CDS             complement(12657..14078)
                     /locus_tag="KR432_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBH0640680.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(14096..14797)
                     /locus_tag="KR432_002150"
     CDS             complement(14096..14797)
                     /locus_tag="KR432_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBH0640681.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14799..16643)
                     /locus_tag="KR432_002151"
     CDS             complement(14799..16643)
                     /locus_tag="KR432_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBH0640682.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5187 a         3122 c         1779 g         6663 t
ORIGIN      
        1 taaaaacaat aaagatttag taaaaggccc tatggatttt tatactcatc caatatatgt
       61 aaagtagttt tttaaaataa ctatatttat aaaaatcatt ttattaactc tttattatat
      121 aagatacaat agtcttactt tatttatgta cacatgcttt taggatgaca acatcaactt
      181 aaatattatg ttactttcta aatttaagta aatattgcca tcctaaaaat tttacaattt
      241 agcttcagat ttaaaagtaa agtttaaaat attatattta aatctaattt aatatttttt
      301 gttttgtaaa tattattaaa ttaatatttc taatatattt tccctggatt taaaatattt
      361 ttagggtcaa aagctaactt tatattttta attaacatca tataagcatc tgaatttgat
      421 tcatgtaagt actctttttt agcatatcct atgccatgtt caccagaaac ttgaccacta
      481 agttctctag attttttata catatagtca aaagtttctt ttaatcttat tttccatgta
      541 ttatcatcca taccatcttt taaaatatat atgtgtagat ttccatctcc tgcatgacca
      601 aaacttttta ttcttatttt taatttgtct tgaagctcgt gagtgtatct tataaactct
      661 gctatcttat ctcttggaac aacgacatca cattcatcca tttgtgtcgt agatgccttt
      721 atagcttcta aaaatgcacc tctggctgac catatagaat cgtttctttc ttgtgtatct
      781 gatataaata catctaatgc accattttct aaacataaat tagctacttt ttcatattcc
      841 ttttctatat cttcagtgct atttccatca aatgtaagta aaagataagc atctgatgta
      901 ttatctggaa actttttacc taaaaattct tctgctgcca atattacatc tctttccata
      961 aattctatag ccgttggtat tgattttgat tttattattt taggaacagt ttctattgcc
     1021 attgataaat ctggaaatgg tataagcaaa cttatacttt tctttggtaa aggaagcaat
     1081 tttaatatag ctttagtaac tattcctaaa gtaccttctg aacctactaa taaatccttt
     1141 atactatatc cagaactatt tttaaccacc tttccaccta catttataat ttctccattt
     1201 ggtagtacaa cttcaagtcc tcttacataa tcccttgtaa cgccatattt tactgctctc
     1261 ataccacctg cattagtgtt tatattacct gcaattgtag cacttttttc acctgggtca
     1321 ggtgggtaaa ataaatctct atcttgaaca tattgaccta ttgtcatcaa taaaacacct
     1381 ggttctactg tcaatgttaa attttcataa tcaacttcaa gtattttatt cattttacat
     1441 agattaatca ttattccacc atttatggca acagcagcac ctacaagacc agtaccttga
     1501 cctcttggtg tgactggtat attattttta tatgcgtatt tcattatttt agatacttgt
     1561 tcagtttcaa gtacatttac taaaacttca gggtacttct caatccctcc aagttcatca
     1621 tgtgaaaaat cttcatttat atcacttcca actaatacat tttcttctcc acaagtatct
     1681 attaaaaaat ctatatcttt tttatcgatt aatttataca tttctatccc cctatttagc
     1741 tgctgatgcc atatattttt gattattatc tttgttattt tctatttttt caatcaattt
     1801 aggaataata tcataaatat cacctattaa agctaaatgt gctacatcaa gtataggcgc
     1861 tttatcatct ttatttattg ctactatata gtcagcaccc tgcatccctg caacaaattg
     1921 aactgctcca gatacaccac aagttattat caatttaggt tttacagttc taccactaag
     1981 acctatttgt tttttagcat ctatccaacc tgcttctatt acaggtcttg ttcctgccac
     2041 ttgagcgttt agcttatcag ctaatttata tatcatctcc atatcttctt gtttttttat
     2101 tgctctgctt gccacaacta tcacttcagc ttcttccaga ccaacttctt tatttttttc
     2161 ttttacactc agtaccttaa ttttagattt tagttctttt ttttctactt tacataaagt
     2221 tatctttcct gtagtattct ctattttttc tggtgctgaa aatattttat atcttacagt
     2281 ggcaaattgt ggtctattat taggtgtgtt tatatgagcc attatatttc caccaaatgc
     2341 tggtcttatt tggtctaaat cagtatttga ttgaatatca agtattgtac aatctgctgt
     2401 aagacctgtt ctaaatctag cggcaagtct tggagctaat gacctaccta atgtagttcc
     2461 accaactaat actatcgttg gttttatttt atctataaag ttttctattg cttttgaata
     2521 tggttctatc ctaaaatctt taaattcatc ttcatcatat acaaatactt catccacccc
     2581 ataagaaatc aaattactgc acttatcttt tatgtctttt cctacaaata cacaatacac
     2641 aggttgtttt atttttgatg ccatctcacg agctttacct ataagctcgt acgttacagg
     2701 atgtatattt ccattatgat gttcaacata tactgcaatt cccctccatt catctttatt
     2761 aatttgtact ttactactct ctataaattc aaatgcgcct tttggtccac ttttgacaca
     2821 caacttgcac atcttacagc cagcacttat ttctactata cctgactttt cttctatagc
     2881 cccaaaagga catatttcta aaattctctt taagtcagtt attttatctt gatttacaac
     2941 tatctttgac atttattact cccccttaca ccaactttaa ttcctctaat ttatttacaa
     3001 gtgcaaaact tagttcatct gcatttcctc ttacaatatt tttatctgta tttggctttg
     3061 gaggaaatat tcttacaact tgagtaggag aaccgttaag tccatagtta ttttcatctt
     3121 tatcttcaaa gtcatctaag ctatatacag gtatttctct atctttagta ttaagtttta
     3181 atttaaatga aggtagtctt ggttgaaaaa tgtctttttc tacagttatt aaacatggat
     3241 attcaatctc acatacttgt aaatcatttg gcatatctat ttcaacaact atactttcat
     3301 ctttaacttc aattaatttt gtaacgttag taacatgagg tatatctaaa aattcagcta
     3361 cttcaggtcc cacctgtgct gtatcaccat cagtagtttg ttttccacaa attattaggt
     3421 ctacctttcc catcttctta ataccttgtg atatggtata ggaagtagcc aatacatctg
     3481 ctcctccaaa tctcctatca cttattaatg ctccttcatc agctcccatc ataaaacttt
     3541 cttcaattac cttctttgcc tgaggaggac ccatgctaag tacttttaga ttcgctttct
     3601 tatcttcctt tattctaaga gctgtttcta aagcatatag gtcataggga ttcatttttg
     3661 aatctactcc atctcttttt agtactcctg tagtttcatc aacttctact gaagtcgttc
     3721 caggaacttg tttaatacat gtcaaaattt ccataaagta cactcctttt ttatttaact
     3781 ataactgata ctccatcagg tataactact actttgctct ccttaccaac tatactattc
     3841 gccatttcta aagcttcttt aaagttaaat gcatgtttca tatgcatttt ttttatcatt
     3901 tcaggattac acttatctgt taccattatc acagtatatt tagataatat tctcgctagt
     3961 atctgaaatt cccattggtc tggaatcgta tctactctat cgacacttct aattttatct
     4021 aaaagttgtt ttggagaatc tgcatttgct acattttcat aaaaagattg acctccatga
     4081 ccatcattac atgctgaaat cattattatt actccaccgt ctttacaagt agcttcagct
     4141 gctgtcattc ctttaacaga ttgataaata ttttggtcta gtggatatcc accatttgta
     4201 gttacaacaa tatcagattt tatagaattt acttttgata actctgttac aaatctacat
     4261 cctttagtat gtgctttttc gacatcacca gcaaaggaag caattatttt tttatcttta
     4321 tctattacaa cattcaatat gaatgctaac tttgcaactt tagctgcata taccatatcc
     4381 tcatgaatag gattattatt caatttccca gttcttgaat tatcactatc tataaagtca
     4441 ctacagtgat tgtacattat agtctttgca gatgcgattc ctggaagtac gctttttctg
     4501 ccacctgaaa aacctgcaaa gaaatgaggt tctataaaac cttctgctat caaaagttct
     4561 gcctcatatg ctagtttgtt tatatataaa tctccacctg aaggcaatat ccctacttta
     4621 cacatagatt tatcatcagt agatatatgc ataactatat cttcattatc tactatttct
     4681 tctcccattt tataaattag ctcttccctt gtacttggcc tatgaaatcc agttgccact
     4741 ataatcttca catcaatttc tggattaaca ttcctaatcc ttcttaatat aattggcata
     4801 gttattctac ttggtacagg tcttgtatgg tcacttgtaa ttatgactac attctttttc
     4861 cccttaacca gttcttccag agaattagag cctataggat tattcataga ttcttcaact
     4921 atctcttctt gagtcttatg ttctatataa ttatccgatt ttgactccaa aacaccgaga
     4981 aaacgttcat caggtatttc taaaatcatt ccttgttttg aatacggtac ttttaacttt
     5041 accattttta atctcccatt ataataaact ataaaattgg tatgtggtca taccagtttc
     5101 acatttatat gatagtacca ttatctacca tttgcaagaa taatttattt tataacaaac
     5161 ttttaatttt cattttttct ataattttta cttataaaat ccatatgttc ctttgctttc
     5221 tcacatgcaa gttctacatc attttctaca actgcattgt atatagcttc atgctgttta
     5281 agcaatatat tttcattaaa aggttctgcg attatttttt ctcttgcatc ttctataaat
     5341 ccatcaaata attttgatgc tgtcataaaa agactttgaa ttaaataatt tttacttaat
     5401 gttgctagtt tactgtgaaa tttttggtct aataacacca gtactcgatt attatctttt
     5461 ttattagttt ctactctcat ttcatctatt atatgtttca attctataac ttcagaagaa
     5521 gttattctct ttgaagcaag ttttgcaatc tcaatttcta ggattattct aagctctaat
     5581 atatcttcaa gtgtgccatt attaagttta aaaatcatag ataaaggttc tattaaagtt
     5641 ttttctatat ttgtacatat aaaatttcct tcaccctgtc tgctttctat cactcccata
     5701 gtctctaata ccctaatagc ttcccttatg gaagttctac tcacattcat ttgttcagac
     5761 aattctcttt ctgatagtaa tttatccccc ttttttaact ctccatttag tattttatat
     5821 tgtatttgct caattacttg ttcataaact tttttactag ctatactatt aaacattttt
     5881 tcctccaata tgataatcgt tgttgtaata tatatctatt atatatctta aaccattaca
     5941 ttcatatatt tttattactt ttaatataat atcttataac tcaaatttac aggtgatatt
     6001 atgaaaaaaa ataaaattat tgcttttttt gctccagcta ttatagttgg agttctagtt
     6061 ttagggatta tcctataccc aaatgaagct ataaaatctg caagagaagg cttctctata
     6121 tggtctaatg ttttagttcc atcactacta ccatttataa ttggagcaaa cttaattgtt
     6181 gatttaaaaa tagtggatat tatagggttt atcatcaatc ccataactag atttgtattt
     6241 aatgtttcag gaaagagcgc tttagctttt gctatatcta cagtatctgg ttatccagta
     6301 ggagtaagtt tagcttctga atttaggagt aatgggcaaa tttctaagca tgaagcacaa
     6361 agacttgtgt ctttttgttc tacatcagga cctttattca taataggttc ggttggtaca
     6421 ggtatgttcc aaaattcaaa cttaggttat ttaatgattc tctgccatta tctaggaact
     6481 atattggttg gtcttttctt tagaaattat ggtaatgaaa atcttccaaa gacaaaattg
     6541 gatttaaaaa caaatataaa aaatgtaatc aacattagaa attcaagtgg gaatggtttt
     6601 tttgttttat ttggaaatgc tgtatttaat ggagtaaata cattacttac agttggtgga
     6661 tttgtaattg ttttttcagt agtatttaaa atactatctc tttttaatgt aatctcatta
     6721 atagcatcag ttatatattt gcctctttca tttttaggtg ttagtaaaga actctgtcaa
     6781 gcatttgtaa gtggtctatt tgaaattact ataggatgca ataaagtatc tagtataaca
     6841 agttctccag aaattttaag agcatcttta gctagctttt tgataggatt tagtggatta
     6901 tccatcctag ctcaatgttg tacatttcta tcaaaaacgg acatagattt aaaattatac
     6961 atactaagta aatttgcgca tggagtttta gctgctatct ttatatttat actttatcca
     7021 attgcaaact ctgctgtgtt ggtttctagc tttgctgata catacaacgt tatatataat
     7081 aatttaatct ggttttatta tcttagctat tatgatacaa ttcttcaaat tgtagtaata
     7141 atttatctaa tctcagcagt atttattgca aaaaaacata tgaaaacttt atctacaaat
     7201 gggaaactaa tgcgatataa aaatactttt tttattaaga aattttcacg aaaaaaataa
     7261 aactaataaa acttaactaa atacttccat ttttttatta tatttgtcat aattttaata
     7321 acctttttaa ttagtttaac agcatagtta tttcatcaca atttaataat tttattagaa
     7381 atatttttca aaaaattctc cttaatcaca tatatcacac ttgtataagt ttactgggaa
     7441 atttttccat tatataatgt ttcatttata ttatataata aataccgacc taaacttacc
     7501 aaatcaaact aagaggagtg gttgaaaatg gcaacaagac ctatagaaat agacgaatat
     7561 aaaaagataa tggaactatt acatactggc tttacttata gtgaaaatgg cgttgaaaaa
     7621 agatttagaa aaaatcctaa agttgcattg gcactaatgt tagaagctaa tttaggtcta
     7681 agaatatcag atattttaag attaaagata ggtaatttta aaggtaatat gttggaaata
     7741 aatgaaaaaa agacgggcaa attacagtat agaccagtta ataaaaatat aattgagtcc
     7801 ataaatgaac atgcaagaaa gtataatctt aaatcaaatg actacttagt aaatataaag
     7861 acaaaggcta ttcaaaaaca gttgaggata atttgtaaac acttaaatct gtataatata
     7921 agtacacata gctttagaaa gctctatgct actactcaat ttgaaaagag taacaataat
     7981 ttagagttgg ttaaagaact tttaaatcac agttctgtag ccactacaca gcgatatata
     8041 agagtaactc agcaagctat taatgaagct agtgaaaatt tctttattgg ttaattataa
     8101 cgtatacatt tttctattat cttattaatt caccagatat taaaaaagag tgtctcaaaa
     8161 tgaacttttt agttcatgag acactctttt tttataaaat caaatatatt tttattattt
     8221 caatactgga aaaaaggctt gtctctctat tttgagacac cctattaaat tttcataatt
     8281 cttgcataaa catattaatc tatcttcaat aattttttag ctgattcact gtaattattt
     8341 ctttttacaa tttccaaaac tctatcaatc tcatgttctg actttgcact tacaaaaatc
     8401 gccaattctt catcatctat aatatcataa ctatctacat atttatctaa acattctaaa
     8461 attccatctt tattattata cccaccaaat ctaactactg cactacactc atcttctata
     8521 agtccactta aatccaattt cccttttgaa aaagacttta tatttgaact tctattatct
     8581 attatatcta taatatctgc ataaacagca cttcctgttg cacctcttcc agctccctta
     8641 cctacaaaag aaagttcacc tactgaatca cctactaaaa taactacgtt gaactcatta
     8701 tctatctttg aaagcatatt attattgtcc actaatgctg gtctaacaaa tccacttact
     8761 tctcctgatt catacttact tctagcaacc aattttatct tacaatttaa tttttttgca
     8821 tattctatat ctttttcatc tattgaagtt attccttcta ataataaatc tttccaatat
     8881 actatttttc cataagataa agtagacaat atagataact ttctagcact atcatatcct
     8941 aaaacatctg catctggatt aacttctgca aatcccaact cttgtgcctg ttttaaagca
     9001 acatcatatg gtaaattttc atcatacatc tttgataata taaaattaca agtcccattt
     9061 aaaatagcgt ttatgctctt tatattgttg ccttccagtg attctatcaa tggttttaaa
     9121 actggaatac cacctgccac agatgcttca aacttgatgc taacattatt taagttagct
     9181 aattctataa ggtcactacc tttttcagcc agtaaatctt tattagctgt cactacatgt
     9241 atttttttat ttaacgctct tgttacataa tcaaaagctg gatttaatcc tccaattact
     9301 tcaactagta tatcaatatc cttgtcaaat atatcttcta tatcagtagt tatcttatca
     9361 aaatacttct tatacttgtg tttttcttta tttttaacta aaattccaac aacttctata
     9421 tctctttttg aagtattatt ttctattaaa tcaattaatc cacttcctac tgttccatac
     9481 cctaatattg ctatcttcac tgtaacatac ccccatcatt ttgattatat taaattattt
     9541 atatatataa atataccaat aaaaaaagct tgagtataaa ctcaagccat atgacgggtt
     9601 tatactcatc tttcagcata aatgctgcag gatttagcac caatgtataa ttaaatatac
     9661 tggttgccgg gcttcacagg gccagtccct ctgccactct tgataagatt gctaattaaa
     9721 atttatgttt aatagtataa tacaatttct tccaattgtc actatttttt taacttattt
     9781 ccctaaaagg atataaacta ttatctaaca gctcttttag ttggtttacc cttttttgta
     9841 acattattat ttttagtctt tgctttcgcc tgttcatgtc ctggaaaaag tattttaggc
     9901 tcttcaatat tcctcttgta aatagtaaat ttaaaaccaa tagcctgcac aaattcagct
     9961 cttaaagctt cacatactgc attagcagtt tctttagtat caagacctgc attttctaat
    10021 atagtaactt ttactaattc tctagttctt aacataccat ctaattgctc taagaaactc
    10081 tctgtaacac cttctttacc aatttgtgta gtaggcttta aagtatttgc taaagatctt
    10141 aaatacgccc tttgttttcc ttttaacata ttctcacctc tataattcta tacttaaatt
    10201 atctataatc tatatttaaa ttaattgtaa aactcaaatt ctaattcata aattttaacc
    10261 acatctccat cttctattcc catttctcta agcctatcaa aaacaccttg actctccata
    10321 gctttttgga aatattgaag ggattccata tcatcaaaat ttacagaata cattattctt
    10381 ctaagtgctt tacctgtaac tacatacaca ccatcttcaa tatctatact taactcttct
    10441 tcagtaccaa tatcaagttc tggtctatac atttcttctt ctgaaactag ctccacatct
    10501 tctacttctt ttagcatttt agaaacatag gctattactt catctatgcc ttgacgagtt
    10561 gctgctgaca ttttaaatac tgtatatcct ctgccttcta attcactctt aaacttctca
    10621 aacttactct catcttctaa tatatcaaat ttatttgcaa ctacaacttg tggcctcttt
    10681 gaaagctttt cattgtacaa tttaagctca tcatttatct tgtcaaaatc ttcaataggg
    10741 tctcttccct ctaatccaga tatatctaca atatgtatca atacctttgt tctctcaaca
    10801 tgtcttaaga actcatgacc taatcctatg ccttcagaag caccctctat aattccaggt
    10861 atatctgcaa gtacaaaact atctccaaac ttagtttgaa caacacctag atttggtgta
    10921 agtgttgtaa aatgatagtt tgcaattttg ggtttagctt tagttacaac tgatagaaat
    10981 gttgatttac ctacatttgg aaatcctaaa agaccaacat ctgctatcat tttaagttca
    11041 agagttatcc atctttcttc tccatctgta ccagattttg caaaagctgg agcttgtctt
    11101 actgcatttg caaagtgttg gttaccttta ccacctatac caccttttgc aactatagct
    11161 gtatctcctt ctttttttaa atctgctaat acaagacctg tagcctcatc tcttattact
    11221 gtacctgctg gtacttttaa tactaaattt tctccatttt taccagctct ttttcctttg
    11281 cttccgtcgc caccattttg agcttgatat tttttctggt acttaaagtc cattagagtt
    11341 ctaagcccca aatcaacttc aaatatgatg cttgctccac gaccaccatc tccaccgtct
    11401 ggaccaccag ctggaacgta tttttctctt ctaaatgcta ccgaaccatt tccaccattt
    11461 ccagccttaa caaatattct tgccttatct ataaacaaat tcttcaccta cttaattaaa
    11521 aacttttaat tagcattacc aaaaatacca ttataaattc atcagtatta taagaaaaag
    11581 gagtgcctca acactccttt tatgtatatt actcagctat tgctactgga tatatactaa
    11641 ctttttttct cttcttgtct tttctttcga atttaacagt accatcaact aatgcaaata
    11701 aagtatcatc actacctttt ccaacattag ttcctggatg tattttagtt cctctttgtc
    11761 taactattat acttccagca gttactaatt gaccatcgaa tctcttaaca cctaatcttt
    11821 tagatataga atctctacca tttttagaag aacctacccc ttttttagat gctaataatt
    11881 gtaaattcat gtttaacata tggagtcacc ccctactttt ttaaaatttt tatatttttt
    11941 gggtattccc gtttaatttc tttaaccgct aattgaaagt gatttagtaa taattgtgcg
    12001 tcttttacat gcgtatcttt gactatacac tctatatgac cttcttctcc tactactttt
    12061 tcaaattcaa tcttttgtaa tttgtctaaa gaatttataa ctgcaatagc attagatgta
    12121 actgcagcac atactatatc atagcctatt tcagcaaaat cagcatgtcc ctttaaacaa
    12181 aaaccttgca acaagaaatc atcattataa taatatttaa cttttatcat aacttaacca
    12241 ataactaagc gtttattttt tctacaacta tcttagtgta tgattgacga tgtccttgtt
    12301 ttcttctgta atcttttttt gctttatact tgaaaactat aactttctta gctttacctt
    12361 gttcaacaac tttagcttga acagatgctc cttctactac tggagaacct aattttaatt
    12421 ctccatcttt agagcaagct aatacttcgt ttaaagtaac aacatcacct gcgttagctt
    12481 ctaatttttc aacgaataat acatcacctt cagaaacttt atattgcttt ccaccagtct
    12541 ttactatagc gtacattaaa tacacctcct cggcctcaaa ctcgccagta caaggtactt
    12601 catttgaaga tttaaacctt tactgtgcgg cattacaatt attaattata aaatatttac
    12661 attttattgt caactatttt atcgaaaata acattggttt tattgtgctt taatgtacta
    12721 tttgcgttta aagaaatctt tatattatat ttttcaccaa ttttatcaat tatatccata
    12781 taatattctt ttatttgttc aaaaataaaa tcattaaatt ctaccattat gtttttgtaa
    12841 acagtatgtt cacttattct cataatttct ttttcaatac tatctagtat ataatgtaca
    12901 gattttattg tttgttcacc atcacaacat ggacaagaca ttaaataata cttatcaatg
    12961 gattcttttt ctctacgcct agcaacttct actaaaccaa gttttgtcat acctaatact
    13021 tcagcttttc ttttatcttt attaaattct tcttctagga tgtgtaaaat ctccttttga
    13081 tgcttctttt tatgcatatc aataaaatcg attattatta tcccagctat gtctctaatt
    13141 ataagctgtc ttacaatttc ttttgcagct tcaagattag ttttataaac ggtttcatca
    13201 agtttaccag ttccaataaa cttacccgta tttacatcta taaccgtaag agcttctgtt
    13261 ttttcaatta ttaaataacc tccactcttt aaccatactt ttttatttaa taatttttct
    13321 attttagatt ctattctgta taaatcaaat acatctctat tttcctcaag acacagttta
    13381 tcaacataag tcttatctat acctcttaaa atagacttaa gttcagagta cttatcataa
    13441 ctattggtaa ttatagattc aatatcatca ttcacattat cttttacata tttagttgca
    13501 aaatctaaag acttatataa taatttcgga cctataccaa gtttatattc ttttaaaata
    13561 ttttcatact ttaatttcaa ctcttctata tctttcttta tttcatcatg agtgcaacct
    13621 tgagcctcag tccttattat taatcctaga ttttccttat ttactgcctt agtaattttt
    13681 tttaatctaa atcgttcttt ttcatcagta attttatttg atattgtagt tctatcattg
    13741 gaaggtatat agacaatata ccttccagaa atactaatct cagtgtttaa ttttgcgcct
    13801 ttagttccta tttcttcttt atttatctga accaaaatat cctgaccaga ttttatagta
    13861 cttccctttt ttaattgtaa ataagcaagt ttcttaaatc caatatctac aaaacaagct
    13921 tctattcctg gtataacttt ttttacgatt ccacgatata tattggatac agtcttctta
    13981 tttaaattgt cttcaacaaa aagttctgtt aatctttcat cttcaagtac tgctgttttc
    14041 tgggacccta ttagggactc aataactatc tttttcacaa cttcacctct cgagcttaca
    14101 taggagtcac taattcccca tcttctaata catataaatc tcttctcaat atatcaacat
    14161 ctaatgggtc aatatcaagt tcaaacatct ctaatatctt tggtataagt atattagtat
    14221 ttaaattagt ttttgagcct gtagctatag tagcagtaag agtaatatgt tcatcttcta
    14281 aatttaaaac atcaaaggtt cttatcatag gtcttatatc tacttcaacc atcttacctt
    14341 ttttattctt ttttgttatg attatttctt tattattcat aaagtcaatt acttttcttt
    14401 ttacaaactc tttcgtcata ggtcttttca aatctatatt aaacaaatat tctccatagt
    14461 ctatcacaga agaaagagaa ggtgtttgtc tatcaatttc ttttgctttg ataaaatcta
    14521 tcccatctgg aagttgaatt gatactttat ttaaaaattc ctctactgat aaatcatctt
    14581 cttctatttc aatatctaca tactcacctt gactttctgt ccctaaagcc aaagcattgc
    14641 cataactcat tttagggtga ggattaaatc cttgagaatg tgataagttt atttcagctc
    14701 ttctaaacgc tctttgtaac aatctctgaa ggtcaagatg tgatatatat atcatgtcgc
    14761 cttccttttt aaattttact cttattatct tactcatatt agcaaagccc ccttcctata
    14821 tcatgtatat ttattccaca accattgcag ttatgtctac aatcagaagt gattttttct
    14881 tttttagcat tttcgtcttc tctaattaag aattttttac taatacctac atctatatgg
    14941 tcccaaggaa atacctcttc atagtttcta tttctatgtg cataaaaatc tatagaaaga
    15001 ttattctttt ccatagcttc tttccatata tctaaattaa aatgttctgc ccatccatca
    15061 aatttagcac ctagtctaaa tgcatcatat ataacttttc ctattcttct atcgcctcta
    15121 gctactactg cttccatcaa actagtcttt gaatcatgat aattgtattt tatattgtta
    15181 tttcttaatt tatttactaa atgtctctgc ttattaataa cttcttctgt agtatcttga
    15241 ccatgccatt ggaaaggtgt aaatggcttt ggtacaaatg tagaagtact cactgtaaca
    15301 ctaaaacttc tctttagctt tcctccattt acttttctat aaatatcaat aactttataa
    15361 gctaatttag ctataccatc taaatcttca taagtctcag ttggtaaacc aatcataaaa
    15421 tacaacttaa cactattcca ccccatctca aaagctcttt ctgttgcatt ttccaaatct
    15481 tcttctgtaa cacctttatt tataacatct ctaagtcttt gagttcctgc ttctggagca
    15541 aaagtaagtc cagattttct tacttgttgt attttatcag caatttccat tgaaaaatta
    15601 tctaatctaa gggatggaag cgaaatccca atattattag atgcatattc atctaccaaa
    15661 taatcagtca actctgataa cttactataa tcactcgtac taagtgatga taatgatatt
    15721 tcatcatatc cagtattctt aactaattta tctaatattt ctttaagtct tttaacactc
    15781 ttttctctta ttggcctgta tatcatacct gcttgacaga atctacatcc tctagtacat
    15841 cctctaaata attctagtac tattctatca tgaacagtat ctatataagg tactattagt
    15901 ttttctggat aatctacagt ctcaacatct tttattatcc ttttagtagg atttttagta
    15961 atcccttctc tattaggagt tacactttga actgtaccat cttcattgta cttgacatca
    16021 taaaaacttg gtatgtatac tccttctatg ctagatattt tatataaaaa atcttctcta
    16081 gtcgttttat ttttcttcca ttctttatac tcatttacta cttctaaatt aacctcttca
    16141 ccttcaccta atattactac atctataaaa tctgctaatg gttcggaatt ataagcacaa
    16201 ggaccaccaa ccattataaa tgggtcttcc aaagttcttt cttcttttaa tattggtata
    16261 ttggctaaat ctaatatatt taagatatta gtgtatgaaa gttcatattg aagtgtaaaa
    16321 gtgacaaaat caaagtttgt tataggctct cttgactcta atgcaaataa tggtatactt
    16381 ttttctctca ttatattttc catatcaact gcaggagaat acactctctc acaaaataca
    16441 tcttcatcct tatttattac atcatataat atatgactcc ctaagtgact cataccaact
    16501 tcatataaat ctggaaagca atgtgcatat cttatcaatt cactattact agtatcttta
    16561 tgaattgaat ttatttcatt tccaagatat ctagcaggct tttctacttt ttttaagatt
    16621 tttttcaagt ctactttatt catttattaa aattcctcca aacaagctct ttatttttat
    16681 tcttatttat aagtagtatt atacacgata aaatattttc tatcaaaaat acaactaaaa
    16741 tattgaattt a
//
LOCUS       DAEPOG010000080        16645 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEPOG010000080 DAEPOG010000000
VERSION     DAEPOG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.080"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            170..1534
                     /locus_tag="KR432_002152"
     CDS             170..1534
                     /locus_tag="KR432_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBH0640683.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1673..2839
                     /locus_tag="KR432_002153"
     CDS             1673..2839
                     /locus_tag="KR432_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0640684.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2956..3738
                     /locus_tag="KR432_002154"
     CDS             2956..3738
                     /locus_tag="KR432_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH0640685.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3857..4510)
                     /locus_tag="KR432_002155"
     CDS             complement(3857..4510)
                     /locus_tag="KR432_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0640686.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4534..5898)
                     /locus_tag="KR432_002156"
     CDS             complement(4534..5898)
                     /locus_tag="KR432_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0640687.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            6556..7986
                     /locus_tag="KR432_002157"
     CDS             6556..7986
                     /locus_tag="KR432_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0640688.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            8089..9192
                     /locus_tag="KR432_002158"
     CDS             8089..9192
                     /locus_tag="KR432_002158"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0640689.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            9207..9443
                     /locus_tag="KR432_002159"
     CDS             9207..9443
                     /locus_tag="KR432_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0640690.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            9460..10509
                     /gene="vorB"
                     /locus_tag="KR432_002160"
     CDS             9460..10509
                     /gene="vorB"
                     /locus_tag="KR432_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0640691.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            10511..11251
                     /locus_tag="KR432_002161"
     CDS             10511..11251
                     /locus_tag="KR432_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0640692.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            11254..11793
                     /locus_tag="KR432_002162"
     CDS             11254..11793
                     /locus_tag="KR432_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0640693.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            11917..14016
                     /locus_tag="KR432_002163"
     CDS             11917..14016
                     /locus_tag="KR432_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBH0640694.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(14283..14798)
                     /locus_tag="KR432_002164"
     CDS             complement(14283..14798)
                     /locus_tag="KR432_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBH0640695.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(14890..16101)
                     /locus_tag="KR432_002165"
     CDS             complement(14890..16101)
                     /locus_tag="KR432_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0640696.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         6310 a         2036 c         2818 g         5481 t
ORIGIN      
        1 aataaattat tttataagct tacttatact taataagggg ctttttttat tggaagattt
       61 atgttatatt gagatgaatt tattatgata taaaaaataa tgttaggata aatttaaatc
      121 tttttctaaa aatgtattaa tataattctt aaagaaagga tttttaacaa tggatataaa
      181 agagttagtt aaaatgcaaa gaaaatatta taatacaggt aaaacaagag atatatcgtt
      241 tcgtatagaa cagttgaaga aacttaagtt agttgtatca caaaatgaag aaaaaatact
      301 tttagcacta aagaaagatt taaataaatc gaattttgaa ggttttatga cagaggtagg
      361 catgttttat agtgaattaa actttgctat aaagaatata agaaaatgga gcaaaataaa
      421 aagagttaaa tcaagtatgg ttaattttcc atctatatcc aaagttgttc cacaacctta
      481 tggtgttact ctcataatgt caccttggaa ttatcccttt caattagcac ttataccatt
      541 agtttggtca ttagcagcag ggaattgtgt tatattaaaa ccttctgaat attctgtgtc
      601 aacatctagt gtagttaaag acatagttga aaatactttt tcaaaagaat atgttgcagt
      661 tgttcaagga agtcaagaag aaagtgaaaa gttactattg gaaagatttg attatatatt
      721 ttttacagga agtactaatg ttggtaaaat aatcatgaaa tcagcatcag agcatttaac
      781 tcctattact ttagaacttg gaggaaaaag tccttgtatc attttaaaag atgctaatat
      841 agaccttact gccaaaagac ttacttgggg aaaactaata aatgcaggac aaacctgcgt
      901 ggctccagat tatgttttag tgcatgaaga tagaaaaaat gagttaatag agaaaataaa
      961 atattatact aataagtatt ttggagataa tccatgtaat aatgagcaat ttcctaaaat
     1021 cataaatcag agacatttta atcggatatt atctcttatt gataaagata agatagtcta
     1081 tggtggaaat tataataagg agacattaaa gatagagcca actatagtag acaatgttaa
     1141 ttgggatgat aatataatga aggaagaaat atttgggcct atatttccca ttttaacata
     1201 taaagattta gatgaagtaa tacaaaaaat tatacaaatg ccaaatccat tggcacttta
     1261 tatttttaca aaaaataagt acctagaaaa taaactttta gaaatgattc cagcaggcgg
     1321 atgttgtatc aatgatacag ttactcatat agctacgaat tacttacctt ttggtggtat
     1381 tggtgaaagt ggtatgggct catatcatgg aaaagctgga ttcgatacat ttactcacta
     1441 taagagtgtg ttaaaaaaat taaatttgga tgtccctatt agatatgctc catacgataa
     1501 taaattaatt aaggttctta aaaagataat gtaggttata ttgtagaata ataaatataa
     1561 ataaaattga gtatttgtat tctgaatata ttttattttt aatttggtat atttgttata
     1621 aaagtggtga ttttgtgata aaatttaatt atatagaaat aaggaggaaa aaatgaaaat
     1681 taattggaat acaaaataca caacaatagc aatttataca tttataatag ctgctagtag
     1741 tattatattt tatctagtat catcacaaat tgatgtattt tctaataatt tagatgctat
     1801 atttacaaca ttacaacctt ttataatagg atttgctatt gcatatcttc ttaattttat
     1861 tttaaagttt tatgaagaca gaatttttat aaaaagtgag aaattaaaaa aactaaaaca
     1921 aagttcaaaa agaggcttgg gtcttttatt aacatatgca acagctacat tgatacttta
     1981 cttatttatg catttcgtat tgccacaagt aattgagagt attgttggac tagcaaatga
     2041 tatacctatg tatgtaaaca atgcaactaa gcttattgat aaattaatga ctgatttaaa
     2101 tcttgatgag caatacttta atttggcagt tgataagtgg aatgagtttg ttacatatat
     2161 tataaagttt gtaacagatt taatcccaat attaggaagt atgttaaaga atgtagcctc
     2221 tagtatatgg aatattgtac taggattaat tgtttcagta tacttattaa ttgataaaga
     2281 aaaattctat ggattaagca aaaaaataac ttatgcagta tttacagaaa aacaagctgc
     2341 cagaatttta gaattaactc atagaagtaa ctatacattt ggaagatttt taggtggcaa
     2401 aatattagat tcattcatta taggtattct aacatttgta atacttacat tagttaaaat
     2461 gccatataca ttattaatat cagttataat tggtattaca aatattatac ctttctttgg
     2521 acctttattt ggagctatac catctactct aatagtgatg tttgtatctc ctataaaagc
     2581 tttctggtta ttgttgatta tattaataat acaacagata gatggaaata ttataggacc
     2641 taaaatactt ggagattcaa ttggtatatc tgcattttgg attttgttct ccttattggt
     2701 tgcaggaaaa ttattaggtt ttattggaat ggttatagga gttccaatgt ttgcagtaat
     2761 ttattctata attaaggata ttgttgaaag taaattggac aaaaagggat taccgacaga
     2821 tacttccgat tacatgtagc taaaaaatat tatgtataat taaaaatata taaattaagc
     2881 aatatttaaa gcactgagaa agttaaactc ttggtgcttt ttagatataa aattattata
     2941 gaagaggtgt tttttgtggc atttatagaa tgtaaattta aatctaaata tactggagga
     3001 gaaactgata taattgtaat tttgcctgta gcaaatggaa aagaactttt tagtggtaaa
     3061 gagatatatg attttgaagg taaatttaaa acattatact tatttcatgg tttagaagat
     3121 gaccaaagct catggattag atatactaat gtagagcatt atgctaaaaa taaaaatata
     3181 gccctagtaa taccaagagt agaaactagt ttttatacag atatggcgta tggacaccaa
     3241 tattttactt ttgtaagcga ggaattacct aaatttgtta gagctatatt tcctttatca
     3301 gataaaaggg aagataactt tgtagctggt atgtctatgg ggggatatgg agctttaaaa
     3361 ttagcacttt caaaaccaaa agagttttcg gcagttgcaa gtttttctgg ttcgcctgat
     3421 atgattcatg agttagaaaa tcaacaaata gaaaccaatg cagacatttt atttcacgat
     3481 tttggtagac cagaagacgc tagaaatagt gagaatgatt tactttacat acttaagaac
     3541 cttaaagaaa gaaatgaaga tattcctaaa atttatcaat tttgtggtga tagagatttt
     3601 ttatataaaa ataatcagat gtttaaaaaa tatgcagagt ctctgggtgt agatgttgaa
     3661 tatgaagagt gtgtaggggg gcatgaatgg cgtctttgga atttatggat aaagaaattt
     3721 atagatataa ttgggtagta cacttatggt attaacaggt atactataaa atatctttaa
     3781 atatgataaa gactgaaatc ctaaaatcaa attatgattt taaaatttca gtctataaga
     3841 atttaataaa attttattaa gttcttacac gagttattat tggagtaaaa ttgtcaacta
     3901 tatctatata gtatttactc aattcctcta aaatctcttc gcataaatcc gtactatgac
     3961 caagattaat aagaaactgt tttgtaaaat gctttattac agcttgagaa gatatatcgt
     4021 acttgtgtga agtataaact ctaattattt caggtttcat agcaagtata ccaatacaag
     4081 aaagtattaa ttcatcatgt tctatagatt ctttatcatt aacatatctt ttcatgacca
     4141 tttcggttac acttctttga tagcttaaaa tagcttcttc ataagctata tccaaataaa
     4201 aatttctata tgcttcgttt tttagaagga aacaaaggtc agcattcata gcaagaaaga
     4261 acatcatcat agtaccttct tcatggtagt tatagattaa agactgcatt tcatttctta
     4321 tatcttcata tattatagct cctatttcgc ttttacttga gaagtaatag cttattagac
     4381 ctaaatttat attagctctt tctgcaattt gcctagttgt tgtcttatga taaccttgct
     4441 caaaaaataa ttctctagca gcacttataa tactagcttt tgtttctttg cccttttctg
     4501 attttgacat atttactgtg tatacctctc ttttcaatct ttttttgaaa atttgctaac
     4561 tataaatatt gtaatagaac cacctattat aggaaccaaa attctgtcaa atacagtaag
     4621 aataccaatc atatttaata atgccaatat aacacttact ataacagcat agatagctcc
     4681 tttttttgta gcgcctttcc agcaaagacc aattacaaat ggtgcaaata ttacacctgt
     4741 acataatgga gccggccaaa acaataattc taataaacta tcaaatttaa gtgctatgat
     4801 tgcacctatt atagctgtca aagctgatgc aatttgagaa tactttagag tattttttaa
     4861 atcattaaat tctatattag gatgtaataa tttgttataa atatctcttg atataatagc
     4921 actcattgag gtcattaatg aatcggcagt agacattatg gcagccaata tagcagctaa
     4981 gaataataca gatattaaag gtggcaaagt agtcgatatc atttgaggta aaacctgaac
     5041 agatgatatg tttggaaata aactaccacc aatagctcca attattccta tagggaaggc
     5101 aagaaataat agtataaatc ctgcaattac actactttta actgcatttt tagcattatc
     5161 agcacattga tatcttaaaa aactttcata agaaaatatt gagcctaaaa ctaatggtaa
     5221 aactgtatac ataaaatcac taatcgattt atttcctgga aagaatggtt cttttaccat
     5281 tgattgtaac ataagtaaag ggttgtcgcc aactaatttt aagcttatat aagctactat
     5341 tggcaaaaac actatacaaa tagcaagttg aataaatgct gatactgcaa ctccccacat
     5401 tcctgatgta taagtatata taattataac tataactcct ataattacag ctatatcata
     5461 aggaatacca aatgcagtag atacagaacc aaaagaaatt aattgactac ccataatagc
     5521 tagtatagat attggtgtta caagactttg ataagctctt aaaaatacac tatgaccata
     5581 tctagtttca atataatcag caggtgttat atcacaagat aagcttctta attttcctgc
     5641 gaaaaaggca tttataaagc aagctaaagc tgcacctata gtagaataca ttccaaccca
     5701 aagtcctctt tcagctcccc attgaactcc accaactaag caccctcctc caaaagatgt
     5761 agcagcaatg gttgcagcta gtataggcaa aggaagattt ctacctgcga ttaggaaatc
     5821 ggatgctgtt ttaacttttt ttgaaaaata aaaaccaatc catatgtata ttgcacacgt
     5881 aacaattaaa agtaacatag tcttttctcc tttataaaat attttaataa aatatataaa
     5941 atatatttaa aatatatttt atataaaagt agtacgatat ttgttttatt ggttttagat
     6001 taatatagct ataataattt atgatattag atggaaatta cgtaaaaata gaaaattgaa
     6061 aattctgaaa tttttgcata ttgatattta tctcatagat tcttttttgt aattgtaaca
     6121 cgcttttatt tttaaatcaa tagtacatgt ataaaaataa aaggaatcta aataagaaaa
     6181 agatattttt aatatctgga tgtagtatag aaatatcttg aataaattat tcttgaataa
     6241 aattataaaa atttaaaatc actagaaata tgataaaata atttaaaata cgcatttcaa
     6301 tgacatttca agacaagaaa ataataaaaa acaaatctaa attaagccta aatagctttt
     6361 tagttaattt tagtagtaaa aaataaaact taaaaagtgt ttgacaatta tgaattttaa
     6421 gaatataatt aaattaaaat tatacacata taaaaatata ctcctgaatc aagaatgaga
     6481 tatttaagat tcaaaatata tattattata cgtgaataat gaaaagtaag aaaattttat
     6541 aagggagggg tagatgtgag tagtcaagaa agcaaaccaa aaggaaatct tatatcaaat
     6601 tttagagatg atatgagaga ccctaagaaa agagcaatat acacatattc aagttctata
     6661 aacttagcgt acaattttat aggttacttt gtatcaactg ttacaaatat ttttttaact
     6721 gatgtggcaa aacttggtgt aattatggca ggtggtatgc aaattataca atctttcata
     6781 aaaattgctg tagctcctat aacaggtaca gtatttgaca aacaaccatt caaaaaagga
     6841 aagtattatc catggcttag atatgcacca gcagcgttag cattaacata tggaatattt
     6901 ttcatagttc cattaatgaa aataagtcca gcattacttt tacctttgat gacagcttta
     6961 ttattaatag catcttgtgc tcagcaagta gttttcacta taatttatgc tatttaccct
     7021 gtagttgcta agacacctag agaccgagtt ataggctcaa ctactactaa tatatttaaa
     7081 gaagttggta aattcttagt aggtttttca tatccgttat tattagtttt atttgcagat
     7141 gtattaggtg gagaaggaat gggttattta ggtacctact tattctttgc agcaggatgc
     7201 cttttagtat tttggttttc ttcaacagag ataataaaat ctggagcaga agaagaagtt
     7261 cttcaaaaag aagagaagcc aaagattaaa gcaagtgaaa tggcaaaagc tctattcact
     7321 aatgtaccac ttatgatggc attttgttta gaatttctta tatgtataag atctatagga
     7381 ttaggacctt tggctccata ttattttaaa tatgtaatag aagatgaacg tggattagca
     7441 gtatttttat caatcatgcc tttggtaagt gtagcattca tgtttttcgc accagtgttt
     7501 ataaaaatct gtagagagca aaaactagct tcaataattt ctttttctat atgtgcacta
     7561 tgccacttat tggttgcatg gactccttgg gggaaaacta ctttaggagt aactatgttg
     7621 ctggcaattg gtggaggttt ttcaaatgta gtatcaataa taaatcttaa tttctttgcc
     7681 ggttcttgtg attatgggca ttggaaatca ggaaaagact tacctggctt aagtatgtca
     7741 ctatatccag ttgcaataca agtaggagta ttacttgcaa ctactataag aacagtttta
     7801 atgaactcaa tgggatatca agctgatatg gtagtcacag aagcagttaa atcaggtttt
     7861 ataaatatga tttcttattc tatggcaata cctttaataa tagctgttgt tatagcaata
     7921 ctatatccag taagtgacaa gaaattaaac caaataagag aagagttaaa tcaaagaaat
     7981 gcttagttaa aagaaagtta agtagatggt taatttaagc tattaaagta agtagacgca
     8041 atagcgttta tataaatcaa aatacatata cagagggggc ttttaaaaat gatagaaaaa
     8101 ttattgagaa ataccgtaaa agaattacat caatatgttc caggtgaacc aatagaaaaa
     8161 gttaaggaaa aatatggtgt aaaagaaata ataaaattag cttcaaatga aaatcctcta
     8221 ggaccatcac caaaagcaat agaagctatg attgaaatgc taaaacaagg tcaactttat
     8281 ccagagccag aagctaacga acttagaaga aaattagctg aaaagctaga tttaaaacca
     8341 gaaaatttta ttgttgctaa tggtgcggat aacgttatca ctctaatagg tgaggctttt
     8401 ataaatagag gagatgaagt tatatattgt aacccaacat ttccatcata tagaactgca
     8461 actataaaaa atgaagggat accagtagaa gtgcctttaa cagaagatta taagtatgat
     8521 ttacaaggta tattagataa gattacagat aaaacaaaat taatatgtgt atgtaaccct
     8581 aataatccaa caggaacaat tgtggatgat aaagagttag aagaattttt aaagaaagtc
     8641 cctgaaaata ttataactat attggatgaa gcatatatag aatttttaac agttcctaat
     8701 tatgtggatg gacttaagta tgtaagggaa aattataacg ttatagtaac aagaactttc
     8761 tctaaaatat atggtcttgc agggcttaga gtaggatatg gtattgctaa agatgagata
     8821 ataagaactt tatttacagt aaaagagcct ttctctgcaa atcgtgttgc tataagtgga
     8881 gcaacagcag cacttgatga tgaagagttt ataaaagaaa catatgaatt aaatagagtt
     8941 ggaatgactt actttaaaga agagtttaca aaaatggggt ttgatgttgt agattcacaa
     9001 tcaaatttcc tttatgtaga tatgaaaaca gatataccta agttatttga agacttgaaa
     9061 aaaagagggt ttgtaataag accaagtgct acacatgcac gtgtaagtat tggaactatg
     9121 gaagaaaata agaaatttgt agcagtttta aaggaaatat taaatattaa aggagagcca
     9181 agtattggat aatagataga aatattatga aacggggtgt agatatgaac ttaaaaataa
     9241 acgcagagag atgtaaaagt tgtgaatact gcgttatatc ttgtaaaaaa ggagcattaa
     9301 aaatatcaag taagataaat aaagaaggtt atgctcatgt agaagttgat gagagtaaat
     9361 gcatactttg tggaatttgc tatcaggtgt gtccagataa tgtttttgaa ataattaaag
     9421 aaaaagcatg tcaagaggct tagaaggagt tgggataata tgaaaaagat gtggaagggg
     9481 aaccacgcca ttgcagaagc agccctaaga ggaggttgtg aattttatgc aggatatcca
     9541 attacaccac aaacagaggt aatggaattt ttatcacata gaatgtctga acttggaaga
     9601 acttttatac agtccgaaaa tgagatggct gctatatata tggtatatgg agcatatgca
     9661 agtggaatga ggtctatgac gagttcttca ggaccaggaa tatctttaaa acaagaaggg
     9721 atttcttact tatgtgcaaa tcattaccct tgtgtaatcg taaatgtaca gagatggggg
     9781 cctggtcttg gctcattgga ctcagcacag acagactatc taagagatac tagaggggga
     9841 ggaaatggag attatcattt gatagtttat gcaccaaaca gtattcaaga gactgtagat
     9901 ttgatgtata actcatttga tgtagcggaa aaatacagag tgcctgtcga aattttgaca
     9961 gaagcagctt taggtcaaat gatggaacca gtagaatttc cagaatttaa gaagagagaa
    10021 ggagatttag gttggactta tgatggaagt aatagagacc atgcaaaggt tgcagataac
    10081 caaaagccaa ctttttgtat ggagaaacgt atgagaataa ctgaaaatga acaacaatgg
    10141 gaagattatc aagtagaaga tgcagaatat gtgtttgtag cgttcggtat accatcaaga
    10201 acaactatga atgctgttag aagacttaga gaacaaggtg aaaaagttgg aataataaga
    10261 ccaataacag tatggccttt cccatataaa gcatttgaaa aggtaagtaa agatgtaaaa
    10321 ggatttatct cagtagaatc aacagacaca ggtcaattag tagaagatgt tgctttagcc
    10381 tcaaagaaag tatgtaaaga aaatgtacct gtgtatggtt tatttagtgg aaatcatata
    10441 ccaaaaacat tgcaagtaat ggacacttat agcaaaataa aatctggcga aatcaaggag
    10501 gtgttctaat atggctacag aaaaattaac acctagcgtt gtttcaatac caaacaagtt
    10561 ttgcccaggt tgtggtcatg gaatagtaaa tcgtataata gcagaggtaa tagaagaaca
    10621 tggatatgaa aaaaatcatg tattaacatt aggagttggt tgtgtctgca atatgaattt
    10681 tagctggaat ggtgataaga tgcaaactgc acatggtaga gcttcatcta cagctatagg
    10741 agttaaagta gcacttccag atacattagt aatgacttac caaggtgatg gagatgctta
    10801 tgttataggt ctttctgaaa ctttaaatac agcatataga aatcataatg tgactgtatt
    10861 tgtaataaac aataataatt ttgcaatgac tggtggacaa atgtcatgga ccactatgcc
    10921 gggtcaagtc acaacaacaa gtgtaaatgg aagagatata aagactactg gtagtccact
    10981 gaaagttcct gagatggtgg caaacttctt tgatgtagca tatgtagcaa gaggttcagt
    11041 tcatagccca aaggaaataa ttaatctaaa aaaatatata aaaaatgcaa tagaagctca
    11101 attaaatgga gagggatatt ccttagttga aatattagca ccttgcccaa ctaactgggg
    11161 tgtatcacta gagaaatcta tcaaatggat ggaagaagaa atagtgccat attatgctct
    11221 tggcgaattt aagcaaaggg atggtgaata gttatggaaa aagagctagt atttgcaggg
    11281 tttggtggtc aaggagtttt aactcttggt ctgattgtag ctgagtcagc acttgaattg
    11341 gggaaacaag taacttggat gcctgcatat ggtccaacta tgagaggtgg aaaagcctat
    11401 agtgtagtaa agttttctga tgagagtata ggtgggcctg atatggaaga aatagatgtt
    11461 ttagtagcaa tgaataaacc atctttagat tatataaatt tagttaaaga aggtggaaca
    11521 gtagttatta atacatctgc aatagatgag aatgtaacat taagagaaga tataagtgta
    11581 gttaaaataa attgtcagga attagctcaa aaagtaaaca atccaaaagc agcaaatata
    11641 gtagtacttg gagcattaat tgctaaaact gggttacttg aaaaagaatt agctttaaaa
    11701 actatgtgtg atttctttga ggaaaaagga aaaggaaagt ttaatgttca aaatgaagca
    11761 gcattcatgg aaggctataa taacgccatt tgataaatta cccttatctt aagtgattta
    11821 tctcaagatt taataatctt tagtgccaag agaaacttca tcttgagata aaattcactt
    11881 aaaatcacaa ctataaacat ataggagcgt gaatttatga gtttggaaaa acttattaga
    11941 ccaaaaagta ttgctatagt aggagtgact gataaacttg gttttggaag aagtgcagca
    12001 cttagtatag taaaaagtaa ggaaacagat agagtatatt atgttaatcc caaaagagaa
    12061 gaattgtttg ggagaaaatg ttataagatg atacaagaag ttccagaagt agtagactgt
    12121 gtagtagtat gtacgcctag aaatgtagtt ccatctgtat taaaagattc tggagagtta
    12181 ggaacaaagg cagcagtggt atatgctagt ggatttgcag aggaaggtac agaggaaggt
    12241 acagatttag aaaatcaact tatagaaata tcaaatacat acgatatgaa aatattgggt
    12301 ccaaactgca tggggttgct aaattgtata gataaggtta atctatgggc aggaggttct
    12361 aaatgggctt tagatactaa aaaacctggt ataggaattg taggtcaaag tggatttata
    12421 actgctgaaa ttgtttcaag tgattatttt aatatatctt atggtttttc aacaggaaat
    12481 ggaaatatag ttacactaga agatgcagta gatttccttg tagatgatga ttatgcatca
    12541 gtaatagcaa tttaccttga aggtttaaaa aatcctcaaa aatttataga tgctttaaaa
    12601 agggcagcac aaaaaagaaa gccagtaata atattgaaat caggaaggtc agaaaaaggt
    12661 gctatttctg ctgcctctca tacaggaaat ttagctggtt cgagtaaagc ttttgaaagt
    12721 atttttgaaa aatatggagt aatttctgtg gaaaatttag aacagtttat gtgtttagca
    12781 caagctttta gtgttttaga tggtaattta cctgcaaatt ctaattttgc agcaataaac
    12841 ttctcaggag gagaaaatac tatttgtgca gatttagcag aagaaaatgg tgtagaactt
    12901 gcagaaatat cttctgaaac taaagaagaa atgaaaaaat atcttccagg atttgcaaca
    12961 cctaaaaatc cactagatgc tacaacagca ttattccatg aaaaggatat gatagtaggt
    13021 ttattacata cctttgaaaa ggatgatagt gttggattta ctatggtagg tgcaaatata
    13081 agagatgaag aaaatgaaat gcatgaaacc ctttgtaaag ctgtaagtga agctagagaa
    13141 caaggattga aaaaacctgt gtttgcagtt cctacacttg aaggaaatag atatttagat
    13201 tataggacta gattagaaga taatcaagtg cctataatgt catcagttgg aactacattt
    13261 acttgtttta ataaaatggc taagtttata gattatgatt attcaaaaag aactttagaa
    13321 tttaaagctg ttaagaaaag agagtcaaat aatgtagtag cattatcaga attagatagt
    13381 aagattgaga tgaaaaaata tggagtacca gttccaggac aaggtaatac aaaaagtatt
    13441 gatgaattag atgaaatctt aaaatatatt aagtatcctg ttgttttaaa aataaattct
    13501 tctgaaatat tgcacaaatc tgatgttgga ggagttaaaa taggaattaa gaatagagat
    13561 gaggcagtag atgcatataa tgagatttta actaatgtaa aaaaagcaaa accagatgct
    13621 aatattgatg gtatattagt tcaagaaatg gtagaaagtg gtatagaaat tattattggt
    13681 ataactaatg atgaccaatt tggaccaatg ttattagtcg gtttaggagg agtatttgta
    13741 gaggtattta aagatactac attatatcca ttacctataa atcatgatga agctattatg
    13801 atgcttaaaa aactaaaatc atttaagctt ttaaatggct atagaggaag tgagccttgt
    13861 gatattgatg ctcttgcaga tatgatggtc aaattaggaa aatatgctta tgaaaataag
    13921 gatgaagtca aggaaataga cttaaatcca gtgtttgtat atcctaaagg aaaaggcgta
    13981 tgtgcagtag atgccttaat agttaaatat aaatagttta taagttttgg tattcaacca
    14041 taattaaagc accgactgaa aatcacattt tgtaagttag atagtgtgat tttcagtcac
    14101 cccaaattta atttaaaaat attttaaaat gaaagtatag ttttgagttt aattgtgaaa
    14161 tagcactgta taaacaataa aatactacgt ataaaacgaa taataaaagg tgttttaatg
    14221 ataagaatca atcttgattt tcattaaaac accttcttta ttatattaaa aatttttaaa
    14281 cgttatattg tcacaggtgg gtttgttgga gctgcatatg tacgattagc aaattcttgg
    14341 tcaacaaaga atagaccacc ctctccagca agtttaacac gttttatatt gtcttcacaa
    14401 tttgtttctt cttctgcttg ttcagatata aaccaaagta agaattgaga agttctaaag
    14461 tctgattcag agtttgctac agctgctaag ttgtttatta aagctgtaac ctttttttca
    14521 tgagctaacg tacgctcaag aatatccatt gcagaagtaa agtctgaatc tggcattggg
    14581 atagcatcaa gctgaacctt tcctcctaca ttatgtaagt atttgtaaaa aaacatagcg
    14641 tggtctgtct cttctttata ttgaacataa aaccaattag aaaatccttt aagtccttct
    14701 gcttcaaggt aagaagacat agaaagataa agatacgcag aatataattc atggttaatc
    14761 tgctcattaa gtaatttttc cattttagct gaaatcattt ttatacctcc aaattttaaa
    14821 tgctcttata aaatataata atattaaatg gaaaaatatt caataaaagt gtttaaatat
    14881 tttacatatt taataaaaat ttatactacc attaaaaaaa ttataactta caatggctat
    14941 agagccaatt aatggtgcat ttgctccaaa atttgaatgc cttacactta tttttttata
    15001 tttagaaaaa cttgctgaag cagtcaattt ttttaataat gtattttcta taaatgtacc
    15061 aggtatatta aagtcatagc ctattattat agaactacaa tcaatcaatg taagtgtatt
    15121 aactaatgca tatgatacgt atgtacaaaa ttcatcaagc aaaaacatgg caagactatc
    15181 tttattattt gcctcatcta ttaacttgac taaagaaaaa tctttacagt taattaatgg
    15241 ggagttagga taaatatggc tcaatgattc aattctttta attagattac tgacacttgt
    15301 ataataatct aaacatccat tgttaccaca actgcacaat ggtccactaa aattaattga
    15361 tgtatgtcct atttctccac tttgtcctag gttgccagta tgaagtttat tttctagaac
    15421 tagaccagct ccaataccat tcattatgtg taggtatata taatttgata tatttttacc
    15481 taaaccatac attttttctg ctaatgcacc cgaattagca tcatttatca aaaatattgg
    15541 aaaatcagac atttctttta taaaatctac tatatttagg tttgatacat ttccaaagtc
    15601 tgggggattt agtatggtac catttatatc atctactgga cctattgaag aaataccaat
    15661 tccaattata tctctcttgt tgattttcat tattttagta aacattcttt ttattatatc
    15721 ttttaaaaca tcatttgata gacatttagg gtaagtttca gaaagttgtt ttacaatatt
    15781 accttttaaa tctgaaataa ttaccttaca tatatttctt cttattagga ttccacaaat
    15841 acaaggcgat atattagaaa tatctaataa aataggttta cgacctgatg aatgtgcatt
    15901 taaagttgat gactctattt ccctaatcat attttcatta ataagttcag taactagatt
    15961 tgataaagtc attttagtaa gtcctgtaga cttagctaaa tcaactctgg acataggtgt
    16021 atttgtagaa agtaatttca atattattaa cttattttta atttttacat ctttgatgtt
    16081 agaaccttgt ttgttactca tcataaatct ccttaaataa agttaagtgt tttatttatt
    16141 ttcaagtata ctatcacctt ataatagagt aacatttttt tacttgaaaa tatcattaat
    16201 tagaatatat cacattatat atcataaaac atataaaaaa atgttagaat taaattaaaa
    16261 gtgaaaagta gattgatata taaatagtaa atacttaatt aaaaaaagat aatgatagca
    16321 ataattattg ttaccaagaa tctactactt caagtgttac cttaggtatt taagttgata
    16381 gtggtttgca cgtaataaac ttgatgtaat atatattctt aataatatcg ataaaagatt
    16441 tacttaaaaa taatatagat tatgtaaaca tataatctat ttattgtata caataaaatt
    16501 tcattttgta aaacagtgaa ttaaatatgt aatatagata tcgattttta ttacaaattg
    16561 ttactaaata taggtacatt agaacaaaaa tctagtaaat tgtaactaaa atgtaaattt
    16621 ttcctttaat ctatacaata ttatg
//
LOCUS       DAEPOG010000081        16609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEPOG010000081 DAEPOG010000000
VERSION     DAEPOG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.081"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            328..906
                     /locus_tag="KR432_002166"
     CDS             328..906
                     /locus_tag="KR432_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0640697.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            936..1082
                     /locus_tag="KR432_002167"
     CDS             936..1082
                     /locus_tag="KR432_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0640698.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            1387..2766
                     /gene="scfB"
                     /locus_tag="KR432_002168"
     CDS             1387..2766
                     /gene="scfB"
                     /locus_tag="KR432_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBH0640699.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2795..3292
                     /locus_tag="KR432_002169"
     CDS             2795..3292
                     /locus_tag="KR432_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH0640700.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3590..5254
                     /locus_tag="KR432_002170"
     CDS             3590..5254
                     /locus_tag="KR432_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBH0640701.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            5267..7717
                     /gene="recJ"
                     /locus_tag="KR432_002171"
     CDS             5267..7717
                     /gene="recJ"
                     /locus_tag="KR432_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH0640702.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7730..8242
                     /locus_tag="KR432_002172"
     CDS             7730..8242
                     /locus_tag="KR432_002172"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH0640703.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            8316..10523
                     /locus_tag="KR432_002173"
     CDS             8316..10523
                     /locus_tag="KR432_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH0640704.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10548..10997
                     /locus_tag="KR432_002174"
     CDS             10548..10997
                     /locus_tag="KR432_002174"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH0640705.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            11012..11626
                     /locus_tag="KR432_002175"
     CDS             11012..11626
                     /locus_tag="KR432_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0640706.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11717..13273
                     /locus_tag="KR432_002176"
     CDS             11717..13273
                     /locus_tag="KR432_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBH0640707.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            13263..14525
                     /locus_tag="KR432_002177"
     CDS             13263..14525
                     /locus_tag="KR432_002177"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH0640708.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14599..16386
                     /gene="aspS"
                     /locus_tag="KR432_002178"
     CDS             14599..16386
                     /gene="aspS"
                     /locus_tag="KR432_002178"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH0640709.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         6659 a         1669 c         3092 g         5189 t
ORIGIN      
        1 tttgatattt tgtaataaat aaaatgggta tctcaaaatg aactttttag tttgtgaggt
       61 actcttttta tatgaaataa aatatatttt tagcctttta acaatgggga aaaggtttat
      121 ttctattttg acataggctt tttatgcata taaagcttga taaaattgtt ttattataat
      181 tgttttgtta tataaataat aataagaaaa ttatataggt ctttttttga ccagttttgc
      241 atttttaaca actagttttg taaagtatat tgaattttct caaaaaagaa tgtactctat
      301 atatgtaaaa tttttttgga ggtaaaaatg tttaaaagat atgctgaaaa aatgacatct
      361 gttttaattt gtaataatat gatagataat aatgaatcta aagtctattc ttatggattt
      421 gaaatattaa tagctttcat agttaatata accaccatgc tgtttatagg ttttttattt
      481 ggtaaattta catatgtatt atttttttta atgtgttatt gtccaataag acaattttca
      541 ggagggtatc atgcagacaa ttattttaga tgcttattaa cctttatttt tataatttta
      601 tctacaatct taattataga aaatataaat atagacttat ttaaaaatat tattatgatt
      661 attgcaagtg ttagttgggt tggaatttgt gttctgtgtc caatagaaca taggagtaac
      721 cctataagtg atagggaaaa gtttgtttat aagaaaactg ctatatttat atctacagta
      781 gtgttattga taacaatttt aagtctttct ttgagtatat ttgtagatta tttcacatat
      841 agcgcatttg ccatgttttg gatttttgtg atgttagtat taggtaagtt aaaagctaaa
      901 gtataagtaa aatatgtatg agggagagtt aagttatgaa aaagtttatt gtaagattta
      961 tgaagtttgc tagctcattg gcacttagca ctgctatttt gtcagctaat agtacctgtc
     1021 cttggattat tcatcagcct aaagtaccca aagaaatcag caatctaaag aagacaaatt
     1081 aaatacaagc acttatttat acaaaattat caggaaaaaa gaaattatta tattttaata
     1141 agattaaaac aatataaaat cagatacaaa atttatttta tttattttat aggagcctaa
     1201 tacctatttg gtaatatatt agtgcgccaa tttaggggga tatagtgtat ttaatgaaat
     1261 gacttacgga gagtaagtca tttttgtatt gtatgtattt tatgtaaact tatgatataa
     1321 tgaagggtaa agtttaatta aaataataaa aaactttagt atagatagta atttaggagg
     1381 tagggtatga gtatgataca taaattttct atgaatggat ataatatcgt tttagatgtg
     1441 aatggtggag ctgttcatgt gttagatgat gttgcatacg atttgttaga tttttataaa
     1501 gaaaaaagta aagaagaaat attagagata ttaaaatcaa aatatcaaga agaaaaaata
     1561 aatgaagcat atgaagaaat attaaatttg gaaaaagaag gtcttttata cacagaagat
     1621 acatatcaat atcatccaag ctttgtacat agagagcctg tagtaaaagc tttatgttta
     1681 aatgtagctc atgattgtaa cttaaaatgt aaatactgtt ttgcagctca aggtgatttt
     1741 ggaggagaaa aagaacttat gagttttgaa gttggtaagg cagcaattga ctatttgatt
     1801 gctaattcag gaagtagaaa gaacctagag atagatttct ttggtggaga gcctttgatg
     1861 aactttgaag ttgtaaaaca actggttgac tatggaagaa gtgtagaaaa agattataat
     1921 aaaaatataa gatttacaat aactacaaat ggtgtactgt taaatgatga gattatagat
     1981 tatataaatg aaaatatgca taatgtagtt ttaagtttag atggaagaaa agaagttaat
     2041 gataatatga gaccaacttt aaatgataaa ggaagttatg atattacatt accacgattt
     2101 aagaaacttg tggaaaaaag agcaaaagat aagtattatt atataagagg tacttttaca
     2161 agagataatt tagatttttc aaaagatgtt atgcattttg cagatttagg atttaaatta
     2221 acctctgtag agccagtcgt tggagatgag agtaatccat atgcacttag agaagaagat
     2281 ttacctaaga tatttgaaga gtacgaaaaa tttgctgtag agtatgcaga tagacaatta
     2341 caaggagatg gatttaaatt tttccatttt atgatagacc taaatcaagg tccatgtgta
     2401 ataaagagaa taactggatg tggagctgga aatgagtatc tttctgtaac tccaaatgga
     2461 gatatctatc catgtcatca atttgttgga aatgaagaat ttaaaatggc aaatatattt
     2521 gatgaagaaa tagtacttcc agaaaattta aaaaatatgt ttagagaagc tcatgtatat
     2581 actaaagaag agtgtaaaca atgttggaat aagttttact gttcaggtgg atgtcatgca
     2641 aatgcgataa actttaataa tgacatatca aaaccatatg aattaggatg tgaaatgcaa
     2701 agaaaacgta cagagtgctc tataatgata caagctaagt taatgttaga aggagcaacc
     2761 aactaaattt atattttaaa ggagttgttt atatatgatt agagattatt tagaagataa
     2821 acctttaatt gacgaaagtg tatttgtggc aaaatcagca gatgtaattg gaaatgttaa
     2881 aataggcaaa gattcaagta tatggtacaa tgcagtagtt aggggtgatg aaggaccaat
     2941 aactattgga gaaaacacta atatacaaga ttgttctata gtgcatggag atacagaaac
     3001 tataattggt aacaatgtaa cagttgggca taggtctata gttcatggtt gtaaaattag
     3061 tgataatgtg ttaattggta tgggttcaat aatattggat aatgctgaaa taggagaata
     3121 tactctgata ggagcaggta cactaattac atctaataag aagtttccac caggtgtttt
     3181 gataatgggt tctcctggta aggttgttag agagcttaca gaagaagata aaaaatatat
     3241 tgatgaatct tatgaatggt atttagaggc agcacaaaat caaaaatatt agtttaaaga
     3301 gtggttgttt caaaacatat ttaaatctgt tttgaggcaa ccactaattt attttattag
     3361 attttgttga tattttagtt ttcaaaaagt atttgattaa accagatata aaatgtcaaa
     3421 aataaaaatt aactgagtta aataatttta ataaaattgg tagtatatgt gataaaatta
     3481 aatattaaag aggatatagg atattagaat atcaattggt gaaataatgt catatacgat
     3541 aaattaaggt gtaagttttt gggtatacct atcttaggag tgaaaagatt tgagaataag
     3601 ttatagaaat ttaaagcgat atagagaaat tggatatgtt ttaataaagt atggttttag
     3661 ttttattgta gaaagactaa atatagaagg aattgcctat aaaataccac tatttgaccc
     3721 accagaagaa ataaaaaata tgacaactgg tgagagaatg aaaagagttt tagaagaact
     3781 aggacctaca tatataaaaa taggacagat attgagtaca agaaaagact tgttagacca
     3841 ggatataatt gatgaaatat caaaactcag agatgatgta gagaaatttg attcaaatat
     3901 agctattgat atatttaaag aagaagtggg tttaagtata gaagaaatat tcttagaatt
     3961 taaagaagaa ccaatagcag cagcttcaat tggtcaagtt tatgaaggtg tacttaaaac
     4021 gggagaagaa gtaatcgtaa aaattcaaag accaaatata gagaaaataa taaaatctga
     4081 tttagagatt cttagaacta ttgcaaatac tttaaaagat ttaaaaaagg attttaattt
     4141 agatttagta cagatgattg aagaatttca aactcaatta atgagggaac ttgattatac
     4201 atttgaagcc ataaatgcaa ctaaattctc taggatattt aaaaatagtg atgaagtata
     4261 tatacctaaa gtatactcag aatataatac taagaaaatt ttagtaatgg agaaagttaa
     4321 tggtacaaag ttaagtgatg tagaaaaaat aaggagacta ggttataaca caaaaactat
     4381 tgttgaaata ggagttaggt cattttttac acaggtatta tctcatggat tttttcatgc
     4441 agacccacat ccaggtaata tatttgtagt tgctaaaaat aagatagcat atatagattt
     4501 tggaatgatt ggaattattg ataataaaac tttaaaccaa ttaaatgaaa ttgcattagc
     4561 aggtgtagaa aagaatgttg acaagataat atatctattg atagaaatgg atgcattaaa
     4621 tggagaggct gatattaagg gacttagaca ggatttactt tatctaatac attattacta
     4681 tgatatttct atagaaaaaa taaatgtaac agatatatta aatgaactat ttaggttttt
     4741 tagacagtat aaaatagtca tgcctgcaca atttgtaact cttgcaaaga cagttataac
     4801 attagaagga acaagtagaa ctttaaacac agatttttct tttggttcta tgggaaaaga
     4861 gtttatgaaa catcattata agagtaaatt taaccctaaa aatgttgttt taagttcaag
     4921 acaaaatgta gaagaaatat tactggacat aaaaactatt ccaaagcaat taaaagcaat
     4981 tttaaaaaat atagaaagaa ataatataaa aatgcaaatt gaagatgtta aaatgacgag
     5041 gttagaaaat tgtataatag agctaacatc tcaaatatct ttgagtttag tgttggcatc
     5101 tattatagta ggttcttctc ttataattgc atctccaaat atagaaaata atatatggat
     5161 aaaatttact gcaatagcgg gtttctttat atcctttata ataggccttt gcttagttat
     5221 aagaagtatt aggtcaaaat acaaaaaaga ttagggggaa ttaaatttga aaaaatggat
     5281 attgaagtat aaaggtaaga tagaggaaag tgaatttagc aaaaaactta atatttcccc
     5341 ggaaatctgc caaattttaa aaaatagagg gatatgtact gaaaaagatt cagaaatatt
     5401 tatgaatcca tctttagatt atttaagaga ccctttttta atgaaagata tgcaaaaagc
     5461 tgttgataga ataaagttag cagttgagaa gaacgaaaaa atttggattt acggagatta
     5521 tgatgttgat ggtgtatcat ctacatccat cttgtgttta tattttaaga gtataggtta
     5581 caatgttgat tactatattc ctaatagatt agaagaaggg tatggtataa atgaggatgc
     5641 tattaagctt ataagctcta ggggatgtga cttgattata agtgttgact gtggaattac
     5701 atcagtgtct gaggtcaata cagcgaatga tttgggcata gatgttataa ttacagacca
     5761 tcatgaatgt caaagtgaaa ttccatcagc ctttgcagta ataaatccaa aacaagaaga
     5821 ttgtaattat ccatttgatt cactatgtgg atgtggtgta gcatttaaaa tgattcaagc
     5881 cttgactcca aaagaagaat ttaaaactag tatgtataat tatttggaaa tagtaacact
     5941 agcaactatt tgtgatattg ttccacttat agatgaaaat agaataattg taaaaaatgg
     6001 tttaaaatca atgaaagaag gcaaaaatat tggattaagg gagcttataa aagtttgtgg
     6061 tgtagaaagt gataagatag gttcttctca tataggtttt gcaataggtc ctaggataaa
     6121 tgcttcaggt cgattgggat attcttactt aggagtagag ctatttacta ctcagtcaca
     6181 agaagaggca gtagaaatag ctagtatatt ggaagaaaaa aataatgaaa gacagatgat
     6241 agaagctaag atgtatcatg aagctgaaga aatgctaaag tctaatagca gatacaatga
     6301 tgacaaagtg ttggttttag ccaaagaggg ttggcaacat ggaataatag gaattgtagc
     6361 atcaaagtta actgaaaaat actacaaacc aacaattctt ctaggtatag aaaatgggga
     6421 ggctactggt tcagcgaggt caataaaagg atttaatata tttgaagctc tcataaagtg
     6481 caaagacttg atgactaagt ttggtggtca tgaacaagca gcagggttat ccttggattc
     6541 agacaatgta gaaattttag caaatgaaat aaataaattt gcagattata atttaactga
     6601 agatgatatg atagagaatg taaatgtcga gtttgaatta caagaaaatg taataaattt
     6661 aaatcttgta gaagaattac ataagttaga accctttgga ttaaataatc ctaatcctag
     6721 atttatagtt agaaattata ttttaaaaga tttgaaagtt ataggtaaga atcaacaaca
     6781 tctgaaatta agtatagaaa aagagaaaag ctatgaatgt ataggattta atatgagtca
     6841 tttaaaatca atgtataaag taggagacaa agtagatgta ctattccaat tagatgaaaa
     6901 taactatatg ggaaatagga aagtacaatt tttacttaaa gatatacgat tggctcgacc
     6961 taaaagtgca agtaatgaca aactttcatt gaaattaatg tcaaaaataa taccaaaaga
     7021 tactcaaagc ctgtataata tatctgtgtc tgattttgaa ttatttgatg gaaatacaga
     7081 tataaatata tttgattatt ttgaaaaaga tacactaata atatctaatt ctataaatgg
     7141 attttataga gcaatgtcag atatatcact tatagatttg gattttaaca taaattacaa
     7201 tattattgaa gatgttagca aaaatactga taaattagag cttatatttt ctcctaatat
     7261 tgataaaata gacttaaaaa gatataataa tattattctt tatgattatt tatacaataa
     7321 aggcgaatat tcgtatatat atgagaataa aagagaagaa agtgaaatta taaaatatta
     7381 taataaaaca gacctattat atttaaagaa tgtagttagt aatatagttc catcaagaga
     7441 tgaatttata acaatttata aacaagcact tataaaaaaa gaaattgatt tggatatggt
     7501 aaatataagg gaaactttta atgtaattcc attaaagttt tttacgatat taaatgtatt
     7561 tagagaatta aatttacttg actttaattt aaattatgaa aaaaattctg tactcataag
     7621 aatattgcca aaaccacaga aaaaattaga cttaaatgag agtttaatac tcaataatct
     7681 aaaaaattta gaaaaacaat acaatagcag ttattaggag gcgtttccca tggatttaaa
     7741 aaactttata aggaatattg atgatttccc aaaaccaggc atagatttta aagatgtgac
     7801 aactctattt aaagatggag atgcatttaa atatgcagtg gattctatag ttgaagaatt
     7861 gaaagataaa gatgtagatt tagtaatagg gccagaagca agaggatttt tgatgggtac
     7921 acctgtagca tatgctttag gagtaggatt tgttccaatt agaaaacctg gaaagttacc
     7981 aggtgaagtt gagagttatg agtatggatt agagtatggt acagatactt tagagataca
     8041 taaggatgca attaaaaagg gtcaaaaagt agcaatagta gatgatttat tggctactgg
     8101 tgggactatg gaagccgctg ccaagcttgt agaaaaatta ggtggagaag tagtttctat
     8161 gcaatttttg attgagctaa aattcttaaa tgggagagaa aagttgtcta actatgatgt
     8221 taattctctt ataaaatact agtactttta taatagttgg tataacacca actattttca
     8281 tatatattaa aattttatac aaaataggtg gttatatgca tgataaagaa ttacaagaaa
     8341 taattgaaaa aataaaagtt tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct
     8401 ataatcttgc aaaaaaagcg catgaaggac aatatagaaa atctggggaa ccatacttta
     8461 ttcatccatt agcagttgca aatattttaa tagagatgga attagatatt gaaactataa
     8521 cagcaggttt acttcatgat gttgtagaag atacagaata tacctatgaa gacataaaaa
     8581 aagaatttgg caaagaagta gcggatttgg ttgatggagt aactaaactt ggtcaaataa
     8641 aatatcgttc gaaagaagaa actcaatctg aaaatttaag aaaaatgttt ttggcaatgg
     8701 cgaaggatat aagggttata cttataaaac ttgctgatag attgcacaac atgagaacat
     8761 taaaatacat gcctccagaa aaagcaaagt ataaagcaaa agagacactt gaaatttatg
     8821 gtggtatagc tcatagatta ggaatttcca agataaagtg ggaactagaa gacagagcat
     8881 taagatttat ggacccagaa gggtattatg atttagttag tagagtatca atgaagagaa
     8941 gtcaaagaga ggattatatt caaggaattg ttgaacttct taaagataag tttaaagatt
     9001 taagtatacc atgtgatgta tatggtagac caaagcattt ttacagtata tatagaaaga
     9061 tgcaaaaaaa acataagact tttgaagaga tttttgattt aacagcagta aggatattgg
     9121 ttgataatgt aaaggattgc tatgcagtac ttggtatagt tcatacatta tggagaccta
     9181 ttccaggaag atttaaagat tatatagcaa tgccaaaacc caatatgtat caatcattac
     9241 acaccactgt tgtaggtcct gatggagaac ctttagagat acaaataaga actcatgaga
     9301 tgcataatat agctgaaaat ggtatagcag cccattggaa atataaagaa ggtatctcta
     9361 gcaaagagtc aaaggttgaa gaaaaacttc aatggttgag acagatgatg gagtgggaaa
     9421 aagatttaaa agacccacaa gagtttatgg atgcacttaa agaagatgta tttaatagtc
     9481 aagtttatgt atttacgcca aaaggagacg taattgaatt accagcagaa tcaacaccta
     9541 tagattttgc ctatagagtt catacaaatg taggtaataa atgtgtagga gctaaaattg
     9601 atggaagaat agttcctatt gattataagc ttcaaaatgg taatattgtc gaggtactta
     9661 catcatctaa ttctaatgga ccaagtaggg attggcttaa tatagtgaag accccaaatg
     9721 caaaaagtag aattagacaa tttttcaaga aggaaagaag agaagaaaat gttgaccgtg
     9781 gaaatacaat attagaaaga gaatttaaga agcatgatat accacaaaaa gaccctcttg
     9841 ttgaaaaata tatggcatta gttgctaaga aatttaatca gccaagtgtt gaggacttaa
     9901 ttgccactgt aggttatggc ggtataatgt catctcaagt ggtatctaag gtaaaagacc
     9961 tatatttaaa agaggttaaa aaagctaaaa aggaacaaca gatacaagaa gaagatttaa
    10021 ataaacacaa tataagtgaa acagaataca gtaaaaagag aaagaaaaac gcatcacaag
    10081 gaattatagt aaaagggctt gacaatatct tagtaagatt tgctaagtgt tgtaatcctc
    10141 ttccaggaga tgaaattgta ggatatatta ctaagggtag gggagtagcc atacacagag
    10201 ctgattgccc aaatgcaaac aataatggtg atttcttcca aaatagattg gtggaggttt
    10261 catggagttc agcaggaaaa ggtaagtttg aagcggaaat tcaaataaaa gctattgaca
    10321 gaagaggaat tgttaatgaa ataactcata ttgttgcaat ggataaactt agtttaaacg
    10381 gaataaatgc aagaaaaggt aaggatagta ttgtaagtat aaacttactt gtagaggtta
    10441 atgacacaga ggaattaaaa attctaatga aaaaattaaa agcaattcct ggtgtagaaa
    10501 gtatttatag aatgacaaat tagagttaaa ataataggga ggaaactatg agagcagtag
    10561 tgcaaagagt atcttcatct aaagttacag tagatgaaaa cactataggt caaataaata
    10621 aggggttact agtattgtta ggagtgactc atgatgataa atcaagtgat gtagattata
    10681 tgattgataa aatattgaat ctgagaattt ttgaagatga aaatgacaag atgaatttat
    10741 ctctaatgga tataggtgga gagcttttag tagtatctca atttacttta tatggagatt
    10801 gcagaaaagg aagaagacct ggatttagta atgcatctag accagaactt gcaaataacc
    10861 tttatgaaga atttgtaaaa aaagcaaaag ataaaggtgt gacagttggt acaggtcaat
    10921 ttgcagctca tatgatggta gagttaacta atgatggtcc agtaacaata ctattagact
    10981 caagtaaatc tttttaaggg ggaaaacttt aatgattata aaaaaatttg tagatagtta
    11041 ttttggagta aatacttatg tattaggtga tgaaaagact aaaaaatgtg cagttataga
    11101 cccaggtgga agtttagtag agacattaag ttttgtaaaa gaaaatgaat taaatattga
    11161 atatattatc cttactcatg ggcatggaga ccatatagga tatgttaagg atattaaaga
    11221 aaaaactggt gctaaagtag ttgcacatgt tgatgaaaaa gaattattaa atgataaaaa
    11281 taaaaatcta agttatacaa tgagatgtgg agcgcaagag tttgatgcag atatctatgt
    11341 gagtgataaa gacaagcttg aacttggtga attaaaaatg actttcttgc atactccagg
    11401 acatactcaa ggatgtatgt gtataagagt tgaagatgaa atgttcacag gagatacttt
    11461 gtttgctgga agtattggta gaactgattt atatagtgga gactttaacc aaatggaaaa
    11521 atcacttaag aaattatgca aatatgaaga tgctgttaga gtatatcctg gacatggacc
    11581 aaccagtact ctaggggttg aaaagaagac aaatccttac atgtaatttt atattagtat
    11641 ttaaaaaagt ataaaatatt taataactat attagtgagg gggttcttct actgttaaat
    11701 agaaggagtt aagttgatgt tgtatgtttt tttaaaagga catgattata agtatgaagt
    11761 agcagaactt ataaaattgt ttaccagtga atttaagttt aaagactcta atgctaacaa
    11821 taatatagag tcaaagcatt taataaatag attgatttat gagaatggtg tattattctc
    11881 aagtactgaa tactatgaaa atggaaattt aaagtacgag tcaatacaga atatagaaaa
    11941 tatgaatctt gaatttttag atggatttga tagtagcaaa acgattgaag aatttcttaa
    12001 tagactagat agtgaaagta agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa
    12061 aagcatgttt gtagtgctta aacaagtatt taattcttat gtaccatggg gaattttgac
    12121 tggtattaga ccagtcaaaa ttgtccacaa tttaatggat aaaaagctag atgataatag
    12181 cataagaacc attctaaaag ataattattt tataatagat aagaaaatag atttagctct
    12241 tgaaatagct aaaagagagc gattatttat gtatcctata gataaaaata aaatttctct
    12301 ttatgtaagt attccatttt gtccaactag atgtgtatat tgttcatttc cttcaaattc
    12361 attaaaacaa tttggacatt taaaaagaga atatgtttat aaattgattg aagaaattaa
    12421 ggggtttgcc aaagttatta aggagactaa gaaagaagtt gaaactctat atattggtgg
    12481 tggaactcca actacacttg atgaggaaga attagatttg cttataaatt cattgttcaa
    12541 tgaattagat ttaagtaaaa ttaaagaatt tactgtagaa gcaggtagac ctgacactat
    12601 tacagaacaa aaactaagag tgttaaaaaa acacaatgtt agtagaatta gtataaatcc
    12661 tcaaactatg aatgatgata ccttggtaaa gattggaaga gagcatagag tatctgattt
    12721 agtagattgc tttaatatgg ctcgtagaat gggatttgat aatataaata tggatataat
    12781 tttaggtctt atagatgaag aattaaatat ggtaaagaac acattggagg agataaaaaa
    12841 actttctcct gaaagcctta cagttcatac attagccata aagagagcat ccaacttaaa
    12901 tataaatatg gataggtata aagaacatct aacacaatat gaagaaatgg taaaaatgat
    12961 agatttatca atgaaatatg caaaagatat gggattaaat ccatattata tgtacagaca
    13021 aaaacatatg ttgggaaatt tagaaaatat aggttatgct aaagaaggat atgagtgcat
    13081 ttataatata cagattatgg aagaaaaaca aagtaactat gcattaggtg caggtgctat
    13141 atcgaaattt gtttatgtag atgaagatag aattgaaaga gtagaaaatg taaaaaatgt
    13201 tgaacaatac attgaaagag tagatgaaat gataaaaagg aagaaagaag aggtatataa
    13261 aaatgttgac taaagctcct agaggaacta aagacataac tccaaaagag gcttacaaat
    13321 ggcgttatgt agaaaataaa tttagagaaa tatgtgcttt atatggatat gaagaaatgg
    13381 taacacctat atttgaacat acagaacttt tcaagagaag tgttggagat actacagata
    13441 tagttcaaaa agaaatgtat tcttttaaag ataaaggtga tagagaaatt accttaaagc
    13501 cagaaggtac agcaggtgta gtaagagcat ttatagaaaa taaattatat gcagatactc
    13561 aaccaacaaa attattttat gtgacacctt gttttagata tgaaagacca caagctggta
    13621 gacaaagaca atttcaccaa tttggaatag aagcacttgg aagtgatact ccttcaatgg
    13681 atgctgaaat aatcgcacta gctgttcaat tttttaatga agttggatta aatgatttgg
    13741 tagtgagtat aaattcagtt ggctgtccag tttgtagaaa agagtacaat gctttattaa
    13801 aagagtatct tgactcaaaa gctgatatat tgtgtgatac ttgcaatgag agaagagaaa
    13861 aaaatccaat gagggttata gactgtaaaa atcctacatg taaagaaaat atcaaagata
    13921 taccatttat tgcagaccat ttgtgtgatg attgtaaatc tcactttgat aaacttcaag
    13981 aatacttaaa ggaaatgaat ataaactttg taattgataa aacaatagtt agaggcttag
    14041 attactatag aaaaactgct tttgaaatta tatctaatga tataggagct caaagtactg
    14101 tatgtggtgg aggaagatat gatggtcttg ttgaacaatt aggaggacca aaaggtataa
    14161 gtggaattgg gtttggacta ggaattgaaa gacttttact tacacttgaa ggtaatggaa
    14221 ttgaaataga aaaccctcaa tctacggata tatttatagt aactataggt gaagaagcaa
    14281 atacaagaag ctttaaatta ttaaaagact tgagacaaaa tcatataagt gcagataaag
    14341 accatataga gagaagtgtt aaagcacaat ttaaatattc agataaaata aattcaaaat
    14401 ttacaatagt gattggagat gatgagctta aaaatgacac tgcgacatta aaaaatatga
    14461 aaacatcaga gcaaactact gtaaaattaa gtacattagt tgaagaatta aagcaaaagc
    14521 tgtaaaataa gaaagtatga tgttttgtgt attagataaa tatatagcat taaaattaaa
    14581 taaaatggag gtagtatctt ggaaacttta aagggtttaa agagaactca ctactgtgga
    14641 gagttgagag aaaaaaacat aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa
    14701 agaaatttag gtggtcttgt atttgttgat ttaagagata caagtggatt atgtcaaata
    14761 gtttttgata cagatgtaga taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa
    14821 tttgtaatag ctgtaaaagg aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca
    14881 actggtgata tagaaatatt tgcaactgaa cttagacttt taaacaagtc agaaactccg
    14941 cctatttata taaaagatga tgatgatgtt tctgaagcac taagattaaa atatagatac
    15001 ttagatttaa gaaaaccatc tatgcaaaga aatttaaaat taagacataa agtaatgaat
    15061 attacaagaa attacttatc aaacaataga ttttgcgaaa tagaaacacc atttttaata
    15121 gctccaacac cagaaggagc aagagattat ctagtaccaa gtagagttaa tccaggaaaa
    15181 ttttacgcat taccacaatc accacaatta tataaacaat tattgatggt aagtggtatg
    15241 gataggtatt tccaaatagt taaatgcttt agagatgaag atttaagagc agatagacaa
    15301 ccagagttta cacaaataga ctgtgaaatg tcttttgtcg agcaagaaga tgttatgagt
    15361 atgattgaag gtttgcttga agcgatattt aaagaagttt tagatgtaga attggcactt
    15421 ccacttccta agatgactta tgcagaagct atgtctaaat atggttcaga taaaccagat
    15481 actagatttg gatatgagtt aacagatata tctgatgttg tttgtaactg tggatttaaa
    15541 gtgtttgctg atgctacaca accaggaaaa agtgttagag gtataaacgt aaaaggcaaa
    15601 gctgacgact ttactagaaa acaaatatca tcactagaag aacatgctaa gacatataga
    15661 gcaaaaggtc ttgcttggat gaaagtagga caagaaggag taacttctcc aatagcaaaa
    15721 ttctttaatg aagaagaaat gaacgctata cttacaaaaa tgaatgctga agtaggagat
    15781 ttacttttat ttgtagctga taaaaactca atagtatttg atgctttagg acaagttaga
    15841 cttgaagtag caaataggct taatctattg gataaaaatg tatataactt actatgggta
    15901 actgagttcc cagtttttga agaagatgaa gaaacaggca cattttcagc aatgcatcac
    15961 ccatttacat cacctatgga tgaagattta gacaagttag aagaaggtga taagtcatct
    16021 ttaagagcga aagcttatga tattgtattg aatggttatg aaataggtgg aggaagtgtt
    16081 agaatatcaa attctgatat tcaatcaaga atgtttaaag cacttggttt cacagaggaa
    16141 agagctaatg aaaagtttgg ttatttatta gaagcattta aatatggtac tcctcctcat
    16201 gctggtctag cttttggtct tgatagactt gttatgttac ttgcaggaac tgataatata
    16261 agagaagtta ttgcattccc taaaaatcaa aatgctgttt gccctatgac aaatgctcct
    16321 acattggcgg aagatgagca attagaagaa ttaagtataa aagttgatat aaaagataat
    16381 gaataatatg tagttgcaag aaactaaaat gtaaaaagtc tggatataaa gtatatttat
    16441 attcagactt tttttattag aattatttta tattttttct ctatatattt tcataaatta
    16501 tccttgtatt ctcagccaat ataattaaaa tactatgttt aaaaatttta cataaacttg
    16561 attgttttag aaattacttc tgtaatgagt acacgatttt aattaaaga
//
LOCUS       DAEPOG010000082        16457 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEPOG010000082 DAEPOG010000000
VERSION     DAEPOG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.082"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(105..1391)
                     /locus_tag="KR432_002179"
     CDS             complement(105..1391)
                     /locus_tag="KR432_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0640710.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1514..4612)
                     /locus_tag="KR432_002180"
     CDS             complement(1514..4612)
                     /locus_tag="KR432_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0640711.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5336..5488
                     /locus_tag="KR432_002181"
     CDS             5336..5488
                     /locus_tag="KR432_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640712.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(5780..6484)
                     /locus_tag="KR432_002182"
     CDS             complement(5780..6484)
                     /locus_tag="KR432_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640713.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6475..8082)
                     /locus_tag="KR432_002183"
     CDS             complement(6475..8082)
                     /locus_tag="KR432_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBH0640714.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8336..9514)
                     /locus_tag="KR432_002184"
     CDS             complement(8336..9514)
                     /locus_tag="KR432_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640715.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9740..10234)
                     /locus_tag="KR432_002185"
     CDS             complement(9740..10234)
                     /locus_tag="KR432_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0640716.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10235..10996)
                     /locus_tag="KR432_002186"
     CDS             complement(10235..10996)
                     /locus_tag="KR432_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0640717.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11719..14553
                     /locus_tag="KR432_002187"
     CDS             11719..14553
                     /locus_tag="KR432_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640718.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14596..15840
                     /locus_tag="KR432_002188"
     CDS             14596..15840
                     /locus_tag="KR432_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0640719.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(16281..>16457)
                     /locus_tag="KR432_002189"
     CDS             complement(16281..>16457)
                     /locus_tag="KR432_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBH0640720.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT         5734 a         2728 c         1882 g         6113 t
ORIGIN      
        1 ataatttgtt ctaatttttt tcatgataag ctttaatact ttatatagta atttgttgta
       61 gaattttata aaatataatg atttatacag ttttataaaa tatcttaaaa cagtatttca
      121 gtaatatttt ttggtcttcc tccaccaata ctagtctctt ttgcacatat tcgacctaaa
      181 cctgcattta ctattttatt taatatacgt cttgcacttc tttcacttac atttagatac
      241 tctgctaact cttttgaatc ataaatctta ctttttctat tctcacttat agcaataatt
      301 tttgcaactg actcacaact cattccagtc tttttagata tttctattac cttttcatca
      361 gatagcatca aagaataatt taattcattt tcggaagcta aaggtcctct tatattttca
      421 tcttcatcta ccaaatatat accaatttct tttgaatcta tggagtgtct taatgcttta
      481 tgtgcattat tttcagcctg atacgcagtt actccagtac caattcctat actaagagaa
      541 aagcccatgt cttttatctc tctctttaga ttgaatagct tatcataatt atactcatta
      601 tcagctaccc ctttatgaac aaatacaaca taatcatttc ttccaagtaa aaataaggaa
      661 ccttgaatgc ttcttacata gtttactaca agcttatcca tatctgattt ttttatcatg
      721 tcagaataat agttatctat cttctcttta taatctatta gactcaatat ttcgacagca
      781 atttgagaaa atcttgcctt gcttagagca tattcactct ttaccttatc ataactttct
      841 ttgattaatt gtatagtagc ttgtaaacga aatacaggat agcctctttc ttttaattca
      901 ttatatactg caccaaaacc agttaacatt atatccgtct tattttcttc aaataatttt
      961 atatgccatt ctacaagttc agactctcct acttccaaag aaaatggatt acaaaataca
     1021 ctcgttgctt taatatcaaa ttctttaagt gcatcttcca caacatatct atcaactaca
     1081 tctatactga atttatctaa ttttatattt gcacttttta ttgcccaaaa tgccttcatt
     1141 atactcgtcc cacttctcgg tacaaatgaa tgtggcttac ttatctcata cttagatttt
     1201 ataaattcat aaactgcaca tcctgtaaat ataatagcat cacattcatc ttcacattta
     1261 gaaataactt ctattgtatc tactactttt tctcttacat atattttagt ctttaaatca
     1321 ctgtctatct gttttaaatc atcctttatc ttaaacccag agtctgaagg acctataata
     1381 cccactatca taaacttcgc tcctaatcca taaatattat actttttaaa aattatatcc
     1441 tcactatttt ctacttctat ttaattatgt tgaactactc catcctacct acatttagag
     1501 tgggataatt agcttatttt tgataaagta acttctcaaa atcagcactt ggaataggct
     1561 tacaataata ataaccttga attaccacac aatctaaact cactaaaaat tcagcttgtt
     1621 cttttgtctc tacaccttct gccactacat ccatatctaa atcttttgcc atggctatag
     1681 tgtgccttaa aactattttt tctctgtctg tgattccttc ctcattaaag aagccacaat
     1741 ctatcttcaa tgtatcaata ttaatatttt ttaacatatt taatgaagaa tatgctgaac
     1801 caaagtcatc catatttaaa acaataccat ccattctaag gtcggctatt atcttagtaa
     1861 ataattttat atttttaata aataaacttt ctgtaagctc caattctaaa aaagattttg
     1921 gtatactata tttatttatt aaacttaaaa caatttcttt aaaatgtgga ttatgcaaat
     1981 gtaatcttga tacatttaca gagataggaa ctggctcttc acctaaatct atccattttc
     2041 taatcaaaga acatacttgt tcccacacat acatgtctaa tctgataata aacccattct
     2101 tctcaaatag tgaaataaat ttaattggag gtattatacc tttttctgga tgaacccatc
     2161 taaccaatgc ttccgcacca acaattttat ttgcattaag attatactta ggttgtagat
     2221 atacatggaa ttgcccattt tcaagtgcgt attccatttc cctttccatc tgtttttctt
     2281 caagtgcatc atttcttata gtatcatcat aaaatatata gttattgata ctttgtttct
     2341 ttactttttt gtgagcatat cctgccattt cacacgcatt tgtaattgga atattattat
     2401 ccaaaatttt ataaacccca aaacatggta ctaacatata ttcaaatttt tttctattta
     2461 agctattttt aatcttataa attaatttat gaacatctct atcagagtaa ctctcataaa
     2521 aaatacaaaa tacatctgca tacatacgac aacatatacc attagaacca atgttttttt
     2581 gaatcaattc tgcaacatat attaaagcct tatctcctcc tctaactcca taaatgtcat
     2641 taattacctt gaacttatct atatccatca ttataattgc atagctcttg tcaggatatc
     2701 ttttaagcat taaacttgtt tgttcacaaa aactttcata attgtatagt cctgttaata
     2761 tatccttttg gtttttatgt agttgcataa gataatctat attttttata gcaactaaaa
     2821 tatgtgtttg tttctcacct aacttatttt tactactaat ctttaacata taaattaatg
     2881 cccaatagta ctttttatct attcctaaac gtctatactc aaaatttagt tctaaatcgt
     2941 cttgcttgct gttattaaga ctatcaagag attttagaag gtggttatca tcaaataata
     3001 ttatatcatc aggatgaatt aaatgttttt ttatgtatga aaaagttttt tcaagatttt
     3061 cctttatagg aaaactaaaa atattgctct ctgattgata aattggagta attaaattag
     3121 tatcgatgca aatagataaa attatatcat atgtattata agctaatttt tcataagcct
     3181 tagaataccc ttttatcatt aaatcatcaa aataaacctt atcaactaac tctttatgtt
     3241 taaataaaat attattcgtc gaatcagcta agtcactaat tacttccatt tcattatttc
     3301 taagtttgtt cttattataa tacctcttct tatcttggta cattctctca tcagcaactt
     3361 taattagctt atgtatatca atattatcgt cactccaaat actgcccact gctacactat
     3421 atatcatctc atccttaaat tcttcactta accaactcac taattcttca aatagttgtt
     3481 ttgatatatc ttcacataat acaataaact catctcctcc aatacggtaa acattataag
     3541 aatgaaagta tttttttatt attacacatg ctgacacaat agcagagtcg ccattagcat
     3601 gtccaaagtt atcattaata tcttttaacc catttatatc tacaaataca actcctatgg
     3661 atttaaattt tcctttttga aaattatcta tatacttaat atatttgttt ctattatatg
     3721 caccagtcaa gaagtcatag tagctcatat aatttaattt attttctact tttctttttt
     3781 tcatctcagt taataaaaaa taagataggg atgtcaaaag agaggtatct ccaacagcct
     3841 tatgtggatt atcaactcca ataaacccta ctactttatc atcttgtttt agtgaagatg
     3901 tcattaaact ttcaattttt tgaggtttta aaatttcata ttctattggc tgttcttctt
     3961 ttatattttc tatatcatca attattacaa aacccttttg ttcgaataat aaaaaccaat
     4021 tctttaattc attaatatca agatttgtta gtgcctgttt ttgttcatca acaccttcat
     4081 tgcaccattc atatgtatta gttacttttt ttctatcttc tgatacttca aaaacatatg
     4141 ctctatcagc tttatgaaat ttaccaatat tggttaatac aagatttaca gcatcagaaa
     4201 aattagattc tgttataaga tgtttaatac atccaactaa taactccttt atctctaatt
     4261 tttctgctat tgacttactt atatcttcct tttctgtaat atcaagagct atttctaatc
     4321 gtgcatgttt gccctcccat ttaattaact tgtcctttaa tataaaatgt ctatttgttc
     4381 gagtattcgt attttcccat atgtaatatt catcttcttt taaaatagag tttgtacaaa
     4441 attcgcaggg agaatctttc ccctgtaatg ctttataaca ttttacatgg gatatatcat
     4501 taattcccat tgcctttttc ccaatttcat taatatataa aagttcatat gtatctaaag
     4561 aagaaacata tacaatttca tccaactcat ctattaaatt tattacatcc atttatatat
     4621 atctcctttt attcattttc cagattgaat ttttaaagct tttaaaattt tgctatatat
     4681 ttggcaatga tgttatactt ctttatgttt taaaatagta ccttaataaa gttctatttt
     4741 ttagtaaata gaattattat tgtgtcaatc gtacaataga gttttgtaat acctaattta
     4801 atatatttat aattaactat cttattatgt gtttacaatt aattctcata taaatgacaa
     4861 atctccacct caattaagta ttccaatcat atatgaagat aatacttcat tcatttaaaa
     4921 atataaaact attatatatt aaaatttctt caataataaa ttatctagat tgttactttg
     4981 atatattata taatatcaca aaggctgata taaaaagaca aaaagggtaa tttttttata
     5041 tcaaaaaaaa tgttaaattg atattatcca tatataaatt gatattatcc atatagatgc
     5101 tatatttaac aattctactt acttccctta cacttattag catatagtta ttaagagttt
     5161 gttgcaaaac aacacttagc acactttaaa aagtgtggtc taataagtaa tcaacgaaga
     5221 agacataata aagcataaaa tatgatatac ttttcctgtc atcaaaactg atgattttac
     5281 tattaatatc tgctaaaata tgcttaataa attaaatttt agtgggagaa tagttatgaa
     5341 aatatttagt ttcttgttgt ttgtagtggg aatatgtttt tgtattgctg gatttatggg
     5401 acattaccca gcatttgctg gtgcaacatt gagttttttt gcaactacaa tgatagctgt
     5461 aagacaagtc aaaactaaga aaaaatgaaa taggcaaatc ctaactttac aagttaatat
     5521 tacataagta attagtctaa aattggatac tttttctatt acagaaatac atcatccact
     5581 ttgtattctc ttaaaaaaat aaaatagcta tgtaaattca agattagata gattaactct
     5641 ctatccattt cattgtaaaa tatacatagc tatttgtatt cgatttaaaa tacttagatt
     5701 ttcttacata acaaataatt tctatctgat tattactcat attcatttag taatcactca
     5761 gcaactaatc atttagtatc tattcatccg ccatacggta acctacccca acctcagtca
     5821 aaatatattc tggctgagca ggattctttt caattttcct tcttatactt gccataaaaa
     5881 ctctaagcga ctgattttca ctttcatagt aatttcccca aactttatct attataaact
     5941 tatgagttaa gactcttcct gaatacttag acatcaactc aataatctta tattcgattg
     6001 gagttaaatg tatttcttca ccattaattg acacaatatg attttcataa tctatcttta
     6061 ggtttttaac ttcaaaagac tttggagcat cattatttat attatcctgt tgagctttat
     6121 ttctaagagc cacacgtact ctagccaaaa gctctgttac actaaaaggt ttagttaaat
     6181 aatcgtctgc tcctccatct aaagcttcca ccttatccct ctcatgctct ctcgctgaaa
     6241 caacaattat tttattagtc tttgcacatg ccctaactcg tcctattacc tcaatcccat
     6301 caatatctgg tagtcctaaa tccaaaatta tcaaatctgg gtcaagagaa tgtataagtg
     6361 atatagctgt tcctccatta tctgtctcta cacacctata attttgagtt tcaagagata
     6421 cttttataaa tttacaaatt ggcttatcat cttctacaat tagaactaat ggctttattt
     6481 ccacttgtct ttcccctcct catcatacat aggtatgcta aatctaaatg tagctcctcc
     6541 agaaggatta ttaaatgcga caatttctcc accatgagct tctactatag atttacatat
     6601 ctccaatcca agaccaatac ctcttctttc aagatttcta gacttattct tagtataaaa
     6661 cctattaaaa atattaccga tatcttcatt tggtattcct tttccattat ctgcaacttc
     6721 aaatagcact tgatttgact ctttttttac agttagttct atcttagaat ttttaggtgt
     6781 atgccttata gcattatcaa ttaaatttac taacacttgt tctattaata atacatccac
     6841 atgaacgatt attatctcat ctggaatatt ggtttttatg tctctactat tggcaaactt
     6901 tttgactctt aatatagatt cagctattat ttcatcaaca acttctggtc gtttttcaat
     6961 atccaatttc ccttcatcta ttctagtcat actaagtata ttctcaacag aatgagttag
     7021 ccaactcgta tcttcatata tatttttcaa taattctact ctagtttctt tatctatcac
     7081 atcatcattt tcaagtattg ttgaagacga acccaatata cttgtaagtg gagttcttaa
     7141 atcatgagaa attgacctaa gcaaatttcc tcttaatctc tctctctctg cctcaagatt
     7201 tgctttttta tttttctcaa ataaattttc tctatcaatt gccaacgcaa cctgtgttga
     7261 aactgcttct aatagtatct tttcattttc tgtaataata tctatcttaa aacacgcaac
     7321 acctataaca ccaagaacat ttgattcttg accttttata gggaaataat atgcactgct
     7381 gtttttgcaa tattttgtcc caattccaac tgggttacct gtcctaaaag cttcttttat
     7441 agcttctttt tctaatactg aatggaatat attctcactt ccatcattgt tacaaatata
     7501 aatacttggc ttacctagat tattattctc atctgcaata gtcactatta cagttctatt
     7561 taatatattt actaaattag acccacaaaa ttcaataatt tgatttttat tttttgcttt
     7621 aagcaacgtt ttgttattct catagagcaa tcttatcatt ttttctctct tatgagaaag
     7681 tctcacttct ctttttatcc tagaagttaa agtacttgtt ataattgctg ccattagcat
     7741 aatacaaaaa gtcataggat aatcttttct ataagctaaa aaagtatata atggatgcgt
     7801 aaagaaaaaa ttaaatgata gcactcctat tactgaagaa agtattccaa aaaaatatcc
     7861 ataggttata cttgtagcaa atagtacacc taatatgtat actagtataa tattagattc
     7921 attaaaacct accttttcaa aaactactga tattccagta gcaattgtca taaccaatat
     7981 agtttttatg aagtccttaa taattatctt tttactgaga tagccatctt catcataatc
     8041 atactctttt aagtcttcat gttgtagtcg ttcctttttc atttactaca attcctcctt
     8101 attgttactg tttaaattgg ctctatttta tataatacaa ttttatcata ataaattcaa
     8161 tcaatacatt taattaatgt acttaaatta ctaaaaggtg ctgtcttaaa atgaaaataa
     8221 atctctattt caaaacagca cctatatatt taaattacta atttagtttt tacatactta
     8281 aatcaatagt tttattcaat tttataaatt ataatacttc aattatggaa catttctatc
     8341 caacaagcat aaatataaag tgggcacata tacctgcaca caaaccacct atggtatgcg
     8401 aaagtatcgc tttaccagcc aatggtcttg catctaaagc atccatcata cctatgtgtg
     8461 tacttaaata tccactccaa cacataccca ttgctgtgaa tactgctata tcattaggag
     8521 ttatagcgcc tgattttata aactctggta ctaaagatat tgctgctcca acagctccta
     8581 atgcagttac agggaatgct attgcctctg gtgatgtgaa tccaaataat ggttctatta
     8641 tgaagctaat cttatcacca atagcaggta ataattttat accttcatat gcagctcctg
     8701 tgtaaacagc ttgacctgta gcagggtctg tagatggtcc aaaagttaat aacataacta
     8761 atgtacaaac tactaaaact cctggtatta tagccatacc catttcaaca cctagcttac
     8821 caccttcaag tatagcatcc aatgttcttt ggaatacatt accatctctt atttctctaa
     8881 actcttcaag ttttgcacct ttatcattta tttgcttttc agtatcattt ttagggtcat
     8941 aattataata ctttttagta aaagtcaaca taattctaac actaataata ctacctatta
     9001 ttgccccaac attacctatt atagctggta gtacatattc tttaccttga gctatcataa
     9061 aagttgttac aataagtccc ataccaaagg ctgttcctaa attacataaa gcaggtactt
     9121 gatacttctt aaaatattgt gtaaatgttt tgtctttagc aaatggtata atagctggat
     9181 tatcagataa atatgttgca actactcctg ctatactagc accaggtaag ccccatatag
     9241 gtctcataag acccttaaat atcttattaa ctaaagatat tactccaaac tctgaaagca
     9301 acgcactaag tgcacctgca agaactgcca tagccattat taaaaatact gtgtctaata
     9361 aaagcgcatg tgctgtactc attattactt taaacatcat tcctgcaccc atgatgcttc
     9421 ctacatatcc aaatcctacc gccaatagta caataaatac gaaggtttcc atacttactg
     9481 ccttgactct tctcttatcc gacagctttt ccatcttgaa atcttccccc ttttaatttt
     9541 aaattaaggt tcctaacatt ggtacaaatt ttttggtaca aactgagttc taaaacttac
     9601 actcgtaatg caagttgtca gttttttgaa ataaaaaaaa attacgttta tagaataaga
     9661 atatcctctt taatttggat actcttattc tattttatgt tattatatta tcatcctttt
     9721 gtttaaaagt caattttttc tatttttgtt gtttaatttt atttatactc tttttatatt
     9781 tttcattttc ttttatcttt gtaagcactc catataagtt gacttttcta aatacagtag
     9841 ttataaatac caatcctaat gcaattgaac ttgcacatga taatgtactc accccatatt
     9901 ctacagcttt tatataatta ttggtcaaaa cagaatacat agtattatat attccataac
     9961 caggtactaa tattattaaa caacaaacac ttaatgtagt tgctggagtc atataaattc
    10021 ttgcacataa ctcacaatat atcgcaaaac aaactgtagc tataaaaaaa cataagtttt
    10081 ctgttactcc catatttaaa agaaatttat atgaaaacca agctatactt ccacctattc
    10141 ctgctagtat taagtttttt cctttcatat taaatagcac tccaaagctt aaggcagtaa
    10201 aaaaagctgc tattacttct ataaacaaat gcacttatat cccccctaac ttgaagtata
    10261 atccaagtac caagaacatt cctactgcta tagatacacc tacaaaaaat acttctgcta
    10321 attttgcaag accagaaatc aactgtccat ctaacaaatc tcttaatgca ttagtcaaag
    10381 caagcccggg aactaagagc attattgctc ctgctattaa atggtctaac gtctttataa
    10441 atccgagctt taaaattaaa attgaacaag aagctataat aactgctcca attgcattta
    10501 caaaaaagtc attaagtgaa gatttactta aatacataat aacaaactta gtcagaatcc
    10561 caacagtaaa tgcagctata aattcttcca catttcctcc aaataatatc gaaaaagttg
    10621 ctgctgcaat acctgcaaaa aatattgtta ttaaatccga gtaatagtta tcttcactta
    10681 tttcatctaa aattttctca cataattcaa tatctaaatt atttatactt attgctcttg
    10741 ataatgagtt aacttcatga accaagttta ggtctactct tcttgagctt attcttttaa
    10801 ttgctgaatg taatttccca tctacaaata cagatgccat tacagctgtt ggagttgcaa
    10861 atacatctac gtggtctatt ccaaaagatt gacatattct agctatcgtt tcttcaactc
    10921 tataggtttc tcctccactt tgcaacatag ctttacctgc atttgaggaa aagtttaaaa
    10981 ttctatctat atccacaaaa catatctccc attcttttat agtattattc atattttaat
    11041 acaagatatt atttatttaa actattttaa ataattcata tccaatattt acattttatg
    11101 aaaaaacttg ttttttatgc ttgtcaaaat caaattttaa gcttgtttac accataaaat
    11161 ggctgaatta atatttgaca aacactttca accatttata taaccttcta aattgtataa
    11221 ctttttacta caaataaaat tatatctctt ttaattcttt ctcttgagta tttaagctta
    11281 tagcaacttc atatttatat atgtacaata taaaacatta tttttatgat acaaaatata
    11341 atttgtaaac gtatatatgt attaactgtg tatatataaa ttattcttaa ccttatcagt
    11401 tttttcatat atcttcatct atatcgacca tatattaatt acattgacca tatactaata
    11461 aatgatatta catagactga agtatattaa aaaacttaaa tttagttatt taacaacata
    11521 atactctaat ttaatctata aaaatccata cctaatataa tgcaactctt attttaacac
    11581 catctattac acaaaacaac atattaaaaa gtaaaataca aaatatccac tataaacctt
    11641 attttaatac tgaatatata ttataatata tttaatgtag cttaaatata gtttaaacat
    11701 aaagaaagga aatggtatat gctaagaaaa gataaagttc tattatctct acataaatta
    11761 tgtgaaaata tgactttaaa agaccttcaa tcagataaaa caggattcac tacaatagaa
    11821 attgctaacc attcatctct tgatagaagt aataccagta aagaattaaa taacctatat
    11881 aatgaaaaaa aagttctgaa aatatctggt aagccaattt tatttctaca tgttagtatt
    11941 atagaaaatt taattggccg tactttgaac agtgatgaat tttctataaa taattgtaat
    12001 gagctactta aatcaagtaa aaatgactca gaagatattt tttcaacatt acttggacat
    12061 gattctagtc ttaaactacc tatagaaaaa gctaaatctg ctatactcta tcctccaaaa
    12121 ggtcttaata cgttactcat aggacctact ggtgttggaa aaagtacatt tgccgaaaca
    12181 atgtacaaat atgctgtaga aagtaaaatc ttttcttctg aaagcaaatt cgtagtattt
    12241 aactgtgctg aatatgcaga aaatgctaac ttattattaa gtaatttatt tggttatgtc
    12301 aaaggttctt ttactggtgc taataaggat aaattcggca tagttgcaca ggctgatgga
    12361 ggcatattat tcttagatga aatacacaga ttaccaccag aaggtcaaga gatgcttttt
    12421 cttttgatgg acaaaggcat atacagacga ttaggagaat ctgacattgt gcacaaagct
    12481 aatgtcttta ttgtgtgtgc cacaacagaa gatatacatt cttctctgct tgggactttt
    12541 ttaagaagaa tccctgttac aatcaaccta cctgctttac aggataggtc tttaaaagaa
    12601 agattatctc ttattaaata cttctttatt atagaatcaa aatacaccaa tgcaactata
    12661 aaagttcaca aagatgtaat taaatcatta ctcctttatg attgttttgg taatataggt
    12721 cagcttaaat ctgacataca acttatgagt gcaagagcct ttttaaatta caaaactgga
    12781 cttaaggata atatagaaat agatttatcc cttgttcctg attacatcta tattggtctt
    12841 ttaaaattga atgaaaatcg tagtaatcta aataaattat cagaacttga ttatatgctt
    12901 ttttatgaat ttaatggtaa taacttagaa gttgaatata caaaagaact atctaaatac
    12961 aatatagata catcaaacaa tggatttaaa tactctacag agattcctta tctggaaaat
    13021 gatataagga gtatgtttat aaattacatg gaaaagtact ctacaaaatt actttcgata
    13081 tcttcagtaa ctcattctga ttatacaagt aatgaaattt ttaaagtaat caatccaaag
    13141 atttactatg ctgttgaaca ttctttaaaa gaggccgaaa aaatatcgag aaacaaatac
    13201 acaaaacaaa cttatattgc tctttcaatg catataagtg cacttataga aaatataaat
    13261 caagacacct ataatcctaa gtacaattta tataatgata aaattttctt agataaagat
    13321 ttaaatatat ctaaaatagt tttaagtata attgaaaatg acttacaaac atctttacct
    13381 aaagatgaag taaaatttat atccatgttt ctaaatagca tgtatcttga accaaacaat
    13441 caatctgaaa gtataggagt cataatactt gctcatggaa attcaacagc aagcagtatg
    13501 tgtgatgtag caaattcatt gcttggtaca aaccattgta atgcaattga catgcctctt
    13561 gatgtaaaag taagtaccat acttgataaa actatagagt tggtaaaaga atgtgacgaa
    13621 ggtaagggtg tacttctttt aacagatatg ggctctctta tagcttttgg agaacttatt
    13681 tcagaaaaaa caaaaatcaa aactcgctca attgaaatgg tatctactcc aatagtttta
    13741 gaagcagtaa gaaaaagtac attaccagaa atgactttgg atgaattagc taattcctta
    13801 agctctttaa atccatatat tggacgagca gcaatcgatt caacagaaat aaactatcct
    13861 gaaaagtcaa gagagtatgt aataataact acttgtataa caggatatgg ttcagctatg
    13921 aaagtagcag actttataaa ttcttcactt aaaaatattc gtaaatacaa tattaattta
    13981 gttcctcata atatagaaag ttttaaaatg tataagaata aatttagtaa tactaaaatt
    14041 ctggcagttg ttggaagtgt agattttaat gtagccaaca ccccttttat acacatagag
    14101 gaattactaa atggatatgg cctagatatg ctaaacaaaa taatagaagg tacattcaat
    14161 gccaatagta ttacaaatat aaaagaaaca aatctatcat acaacagttt aataataaac
    14221 tttatgaccc aaaacttaga acttttggac tctgttaaat tattcaacta tgtcaattct
    14281 tccttattta caataataga attaatagat gaaaacttaa agagcaaatt taagctagga
    14341 tatatactac attgctcttt tatgatagaa agatgtttta aaaatacaac ttttccatat
    14401 aataacatta ctgaattaat tagtaaaaat catgaactat atagtttaat acgtgaagcc
    14461 tttaagccaa cagaagctta ttttaatatt ttgataccag acgaagaaat tgcttatata
    14521 atagacatga ttaatgagta tctaaataaa taaaatttat agcacttatt ttgtgcatat
    14581 taattaatat atgaaatgga ggacgagatt ttgaaaggaa aaataaaaca gttaacaata
    14641 cttgcattaa tttttatatt tataactcct gtatttgcat ttgcagatac tccaccagta
    14701 ccaaattctt caagagcagc actacttata gaccaggaaa ctaaaagaat attatttgaa
    14761 aaaaacattg atgaaaaaat gccattagcg agtttaagta aaatgatgac ctttttattg
    14821 gcaatagaag cagttgataa gaatcaagta aaagagactg atatggtgaa aatagacaag
    14881 tctacagctt cagtaggagg ttctacttgt aaacttaaag atggtgatga aatatctcta
    14941 ggagagctta tgcagggtct aatgctagta tctggaaatg atgctgctat agccatagct
    15001 aaacatattg gtaagactga aaagaatttt gtaaatatga tgaacaagaa agcagaagaa
    15061 ataggaatga ttgatacgta ttattttaat ccaaatgggc ttcctatata tactgaccca
    15121 gaacataaag aacctcctat tgaaaatatg tcaactgctc acgatatagt tacacttgga
    15181 aagtatatgt atgaccatta tgaaaatcaa gttacaagaa taaccactat gcaagtatat
    15241 aatgatacta aaaaggattt tacacattat aacacaaatc ccctacttgt ttcagtacct
    15301 ggtgttgatg gaataaagac aggctataca gataatgctg gatattgcct tgcattttct
    15361 atgatggtac ctaaagatgc taaaaacgaa agaaatcata gattgatagg tgttgtactt
    15421 ggtgatggta acaagaaaaa tagaatttct tcttccgcta ctttattgaa atatggtaaa
    15481 gataatttcc attctaaaaa gatagcacat aagggtgata ttatagagac tccttgtgtt
    15541 gatggtattg atgattttaa gataacagtt aaggttgata aagatttgta tggtgttgtt
    15601 tcagataacg aaaatatcaa tcctaaggta gtgtttaaaa atatgaatta tcctatccat
    15661 aagggagata ttgttggtgt tgcaaagtat tataatgact ctggtaaatt tgttggaagt
    15721 gttgatgtaa aaagtgaaag taatatagga tgtattcctt tgaaggataa gattaagatt
    15781 aaggttgcta agataaataa aaaacttgag attaaaaatt ctgtttgttt taaagtatag
    15841 taaagcatac tattaaaaga gtatattaaa tttataaata ctccactttg tggagttttt
    15901 ttgtttttta caaaaaatta aaatagtggc aacaattgag gatgtgaggg agagttgcca
    15961 ctattttgta tagggtatta tattatgttt ttaagctagt tttactatct tctatgttta
    16021 tataataact cttgtgtgtg aatttaattg ataattaata tgaaaattat gtgaagtttt
    16081 gatattaggt tatactttga actttatcta acattttaaa ttttatatct ctaaattatc
    16141 taaaattact atatcaatta actttacatg ataaaaaggc tgtgtataaa tttattttta
    16201 aaaattttac tcacagcctt aaattaaatt ttctatttaa agtatatata aattttatta
    16261 aataatttgc tttttatttc ttatttcata aatcctagta ttcttgataa cattaatgca
    16321 attgtagtag gtactttccc tgcttcaaat gtagctgttg ctgtgtttaa tgctgttact
    16381 gcatcatcta tttctttttg tgttgcatct gctttatcta atattgcttg tgcttcatct
    16441 attgctgttt tatatgt
//
LOCUS       DAEPOG010000083        16279 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEPOG010000083 DAEPOG010000000
VERSION     DAEPOG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.083"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            238..1434
                     /locus_tag="KR432_002190"
     CDS             238..1434
                     /locus_tag="KR432_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0640721.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(1546..2892)
                     /locus_tag="KR432_002191"
     CDS             complement(1546..2892)
                     /locus_tag="KR432_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0640722.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(3025..3777)
                     /locus_tag="KR432_002192"
     CDS             complement(3025..3777)
                     /locus_tag="KR432_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0640723.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(3936..5513)
                     /locus_tag="KR432_002193"
     CDS             complement(3936..5513)
                     /locus_tag="KR432_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640724.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(5926..6378)
                     /locus_tag="KR432_002194"
     CDS             complement(5926..6378)
                     /locus_tag="KR432_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0640725.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(6689..8134)
                     /locus_tag="KR432_002195"
     CDS             complement(6689..8134)
                     /locus_tag="KR432_002195"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH0640726.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(8278..9186)
                     /locus_tag="KR432_002196"
     CDS             complement(8278..9186)
                     /locus_tag="KR432_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0640727.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            9443..10267
                     /locus_tag="KR432_002197"
     CDS             9443..10267
                     /locus_tag="KR432_002197"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0640728.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            10307..10837
                     /gene="lepB"
                     /locus_tag="KR432_002198"
     CDS             10307..10837
                     /gene="lepB"
                     /locus_tag="KR432_002198"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0640729.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(10919..12700)
                     /locus_tag="KR432_002199"
     CDS             complement(10919..12700)
                     /locus_tag="KR432_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0640730.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(13335..14876)
                     /gene="rny"
                     /locus_tag="KR432_002200"
     CDS             complement(13335..14876)
                     /gene="rny"
                     /locus_tag="KR432_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBH0640731.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            complement(15052..16098)
                     /gene="recA"
                     /locus_tag="KR432_002201"
     CDS             complement(15052..16098)
                     /gene="recA"
                     /locus_tag="KR432_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH0640732.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
BASE COUNT         5285 a         2629 c         1927 g         6438 t
ORIGIN      
        1 tatcagtgta tcagaatatt atcttactat aaattaattt atgaatacat aatttagtgg
       61 cataaacatg ggtattttac tataatttgt taaaagttta ttttaaagct atattaattt
      121 tttttgatga tttttaccca taagtatgtt attattatct tgtaaagatg tcttgtattc
      181 aaacatacaa tttacataat acaatatacg tatttaaatt aaataggggg tattaaaatg
      241 aattattcaa acagagttag tgcaatgcaa gcctctccaa tcagaaaact tgttccattt
      301 gcacaagcag caaaagataa aggaatcaaa gtatatcact taaacatagg acaaccagac
      361 ataaaaactc ctaaaggatt ttttgatgct gttaaaaact ttgatagcga agtcttagaa
      421 tatgctacat cagaaggtat tccagaatta ttagaagcac ttcaaaatta ctataaaact
      481 tacgatatga attttgaaaa agatgaatta ttagtaacta atggtggtag tgaagcttta
      541 cttttcacta tgatggcagt ttgtgaccca ggagataatc tattagttcc agaaccattc
      601 tatacaaact acaatggatt tggtcaatct gtaaatgtag aagttaatgc tgttacaaca
      661 aaggcagaaa acggtttcca ccttccatct aaagaagaaa tattgtctaa agtagatgat
      721 aaaactaagg ctataattct ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa
      781 gaagaactta atatcctagc tgaaatagct aaagaaaaag atttatggat aatagcagac
      841 gaagtttata gagaatttgt ttatgatggg cttgagtata caagctgtgg taacttagag
      901 ggtgtacagg atagagttat aataatagac agtgtttcta agagatacag tgcttgtggt
      961 gctagaattg gttctatcgc ttgcaaaaat aaaggtctta tcgctcaaat acttaaatta
     1021 tgtcaaggaa gactttgtgt tcctacatta gagcaaatag gagctgttga gttatacaaa
     1081 actcctgttt cttacttcaa agaagttaat gaagagtaca aaaaaagaag agatgtatta
     1141 tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat
     1201 atagttgcta aattaccagt tgaaaatgca gaagatttca caatttggat gttaaaagaa
     1261 tttaacaaag ataatgaaac agttatggtt tgccctgctg aaggattcta tgctactcca
     1321 ggtttaggac gtgatgaaat aagattagct tacatcttaa atgaaaaaga ccttcataga
     1381 gctgctacat tattaaaaga aggtttagag caatatgtag ctttacaaaa ataatttttt
     1441 aaacttaatt tatataaatt taatgatata aaatgtcccc ttttcagata ttatttgaaa
     1501 aggggacatt tcttaattaa tatattaatt aagacttaga cctgactatt tttcattttc
     1561 tttatatagt gttggccagt aatctttatt cactttaatt aaagcatcta aaacttgtct
     1621 tgccttagtt ggagagatta tagttctatt taatgttaaa gataaaatag ctttttcata
     1681 agattgttca aagtatgcat caactgctaa cttttcataa gctagttgac cttctaacag
     1741 tcccttataa aatgttggta tattacctac atgtaaaggt ctcggtccat tctttgtaag
     1801 aatcgcagct acttctacca ttgcatcatc aggtaagttg ttaatagccc cattattttc
     1861 tgttataact atggctattt ggtgtttgtt atgagctatt gcttctgcaa cttcaactat
     1921 aaattcccca tgagcatcat tatgcactaa attagaacct atagatgttc ctttttctat
     1981 aacagattta cattcttcaa atacacgctt ttctcttcca ttcataactt ctcttgctct
     2041 agtacaattt atatttgatt tagctacttt tttatcagga tatagataat actgtacata
     2101 tgtattaggt aagtaatctg gtacatcttc caacatatct ttaaccattc cataagtgtc
     2161 taaccaagat ttatctcttt gttctgcatc aacaggttgg aatccagagt ttgctactaa
     2221 atcttttatc ttaggtacca aatcttcacc atttttatca tacaaatgtg taaaccatcc
     2281 aaagtgattt aatccaaagt atacaggctc aaatgttctt gaatccatgt ctaacaatct
     2341 tccataagac cttaacaagt taactggttg gtcacacata ttaagtaatc ttttatcatc
     2401 tggaaatact ctttttaatg cttctgcaac aatagcagca ggatttgtat aatttaatat
     2461 ccatgcttta ggagagtatt ttcttacagt cttaaccatt tctatcatat cacctataga
     2521 ccttaaccca taagcaaatc caccagctcc acaagtttct tgaccaacca gtccaaattg
     2581 tagaggaatc ttttcatcta attctcgcat cttaagacca ccagttctca tttgaacaaa
     2641 tacaaaatct acatctataa atgcttcatc tgggtttgta gtatattcaa gtgttgcttt
     2701 aggatattct tctttaaata atatttttcc aaattcccct attaatcttt gacgttcttc
     2761 tactacatct aacatagtta ctttatttag aggaaatcta tcactcattt tacataaaga
     2821 ctttaaaagt cctggagtcc aagtactgcc tcctccaact atagctatat tgtatctttt
     2881 tttcattgtc atattaatca cccctgtttt ttattaacta acttgtaaga tgattataat
     2941 atataaagtt ttctacatga atacttttag gtaatcatta aaaatagttc aattttagta
     3001 tttttttcaa aattgtaatt tttttcatct attgtatttt tcaatgtatt tatgaaacat
     3061 atattccata agagaaagtg ctggcactct agacgtatag ttaacactat taaaattata
     3121 ttgtggagaa gatacaaata attttatatc agctatattt gataatgaat tgacacttat
     3181 atttgtcaga gaaattactt taacttgatt tgtttttgct atattacatg ccttaataac
     3241 ttgagcagtc tctccagaca tagatacagc aattacaagg tcatctttag taatatttga
     3301 tgcagccaaa tacatgagag ttgaatcatc atagtatttt acatttacac ctatagctga
     3361 aaaacgttga gacatttcag tacatactaa tctagaagac ccaacaccaa aaaaatatat
     3421 atgcttagaa ttatgtattt catttaaaca catatctata ttctctacat ttattaaatt
     3481 ttgtgtatcc ataacatcag taaaaatatc actttgactt ttagtaaact ccttatttct
     3541 ttcatttcta tttgaatatg aaaaaactaa aaagttttta aattcactat atccagaaaa
     3601 gccgagcttc tgacaaaacc ttactatgta tgcagtcgaa ttaaaagttt catttgctat
     3661 atctttaatc tttttttgat ttattttttc agaatttttt agaaaatagt ttaaaagaga
     3721 tatttcattt gtagataatt ttttttgatt tatatctaat ttgtctatta ggttcattta
     3781 tacctccata ataatgattt gtagttaaac aactttctca tatttattat ataaatatac
     3841 actatacaac atttatctta tgtttattat actacaaaaa agacaataca aattataaat
     3901 tagtattgcc ttttaatatt tacattagat agacattaaa tagacattgc aaaagaaact
     3961 tcgtctctta cttgagatac agatggccca taaattattt ggatatcttt atcagatact
     4021 tttataacgc ccattgcacc tgtatatttt ttaaaagcat tttcatctac tttactcata
     4081 tctttaacta ctactcttaa gcgagttata cagtttgtta tatcttctat attctcctta
     4141 ccacctaaat atttaattat ggataatgct ctcttagtag tttctccact ttgagctatt
     4201 tctactttat ttgataaagg ttctaaatca aaatttatat ctgttatttc ttctcgtcct
     4261 ggagtttgta agttaaatct aagtatcaaa accttaaata cattataata aaccagtgca
     4321 tacactaaac caagtattat agaataaatc cactttgtat ttggttgcat aactccaaat
     4381 actactaaat ctattatgcc accttgagta tttcctattg ctactccaaa tatatgcatc
     4441 atcataaatg atatacctga taaaaatgca tgtataacaa ataatattgg tgatacaaat
     4501 aagaatgaaa attccaatgg ttccgtaatt cctgttacaa atgttgctaa agcaccagct
     4561 agtaatatac ctttaacctt cgacctattt tcaggctttg cagttttata catggcatat
     4621 gctgcagcag gaagaccaaa taccattata ggtattttcc cttgagctaa aaatctagtt
     4681 gcttctgttg aaaaactgat tgtttcaggg tcacttagtt gagctaaaaa tatgttcaat
     4741 gctccaaata cttcttgtcc tcctacaact aaagaacctc ctacttcagt aaatctaaac
     4801 atagcattta atatatgatg taatccaaat ggtattaaaa gcctttcaag aaatccaaat
     4861 acaaaggttc ctaaatatcc taaactagct ataccaatac ctacactctg aataattgta
     4921 tttacatatg gccatatata tggtattatt atccccaaaa tagagaatac tactgttgtt
     4981 attattggaa caaacctagt tccaccaaag aaccctaaat aatctggcag ttttatatta
     5041 tagtatttat tatgtaatga tgctgttatt ataccaacta caattccacc aaatactcct
     5101 aactctaatg tttgtatgcc taaagttaca gcttgaccca tttctctcat catgtcaggt
     5161 tcagcaagaa tacctctaca ccctaacata taatttacac tagtgtgtaa tactaaataa
     5221 ccaacaaaac cagaaaatgc agaaactgct ttttcttctt tagcaagacc aacagatatt
     5281 gatatagcaa agaatattgc taagtttcca aaaagtactc ctgttatagc ttttgtaaaa
     5341 cccaatattc cttgaataaa attattttct aaaaaaggta tatcactaac tatattagga
     5401 tttgaaaatg ctgaacttat tcctaaaaat atacctgcag cagatattaa tgctattggc
     5461 aataaaaatg attttcctaa ctgtaaaaaa aactctttca ttttttgttt catttctatc
     5521 cctccatttt taaattttta aaacgttttc tatgtcataa ttatatattt ttattttgtt
     5581 tttgaaactt tataatttgt tttaaaatat ttttacataa atagtaattt tttcaaaact
     5641 gtaaattttt tcaaacaaag acatagtaac attgtgatat atagaaattt aaaaagagat
     5701 tagaaaataa aattattgaa tataaaatgg aaaaatataa attacacaaa gaggtatgag
     5761 taggtactaa caaataagaa aatgtataag aaattataaa aaagtacatc aagaaaattt
     5821 aagatgtact ttttagattg tatttagact tataattata tagttaaatg aataacaaag
     5881 aattaatctt agtactatta aatataaaaa ttttttatat tttttctatc taaattcttc
     5941 tttgtttaat atatattgaa gagtatttat tgcttctaaa tatttttttc tgtcttcttc
     6001 agttaattta gaaagttttt ctcctaaacc atcacttaat ttatccataa aatcagttgc
     6061 tatcttatct ccctcttcag taatgtcaat tagatatttt cttctatcaa ctaaatctct
     6121 tttccttgtt acatacttct tatcagttaa atcatcaacc atactagtta aactgccttt
     6181 ttctatattt aacttagagc ataaatcagt catacttatt actccataat tttttatgaa
     6241 aactaatgct cttaattgtg ttttatttac attataatct ggtgcaaatt tttttaggtc
     6301 ttctaagtac agactagaat aaatcttagg aaatgttttt actaaaaaac ttatggtatc
     6361 ttttactata tagttcatat aatcacctcg gttgttttaa aatttattaa attatctaaa
     6421 taactttatt tcagtttaca ttaaaaatat tgcaagtaca atagtttttg ctgaatgttt
     6481 aaatttaatt agtaatctta tcattttttt atttttattt atattatttc tattaaatat
     6541 tatttatata gctattttat taaattcatg gtatagtaag taaagaagaa ctcttcacag
     6601 cgctgaagag aagacgctag cccattaaaa tggctatctt atttataaat aagatagccg
     6661 tttttaatta tattttatct gtacaattct atactcttag gtccacatct acttttccta
     6721 agtcttcctt ataatctttt atagcaggtt ctacaacttc atttacaaat tctacaactt
     6781 gttctggagc tctacctata aagttttttg ggtccattat agatagtatt tcttccttgc
     6841 tcattttaaa tgaatcatca ttcattataa gttcaattaa gttattatcc ttaccttcgt
     6901 gctttactct ataggcagct ttcatagaat attctcttat tatttcatgt aattcctgtc
     6961 tatctccacc tctttttaca gcttccataa gtattgtttc tgtagccata aaaggaagtt
     7021 cttcatttac acgtttattt atcatatttt catatactac taaaccatca gttacattta
     7081 tccctatttc aagtatagca tctgctgcca taaatgcttg aggtattgcc aatctcttat
     7141 tggctgagtc atctaatgtt ctctctaacc attgagtggc ttgtaccatt gcagggctta
     7201 tagattcaga gattatatac ttagataaag atgctattct ctcacttctc attgggtttc
     7261 tcttataagc cattgctgat gaacctattt gatttttttc aaatggctct tctaattctt
     7321 ttaaactttg taaaagtctt atatcgttag tcatcttatg catactttga gctatccctg
     7381 atagtatgct tattacttga taatctaact ttctagtata tgtctgtcca cttactgcat
     7441 aggaagaatg gtatcccatc tttttacaaa ttttcttatc taactctttt actttttcgt
     7501 ggtctccttc aaataagctt aaaaaacttg cttgagtacc agtagtacct ttaactcctc
     7561 ttaacttcat gttatctatt ctatagttta aatcttccaa atcaagaatt aaatcttgag
     7621 cccataaaca agctcttttt cctactgttg taagttgagc agcttggaag tgtgtaaatc
     7681 caagagctgg catatctttg tacttaagtg caaattcttt taaattgttt attaaattta
     7741 caagctttac tcttagaagt tttaaagctt cattcatttg tataacatct gtattatctc
     7801 caacatatgc agatgttgct cctaaatgta ttattccttt ggctttttca cattgtaaac
     7861 cataagcttt tacgtgagac attacatcat gtcttacttc tttttctatt tttctagctt
     7921 catcaaaatt tatattatct atattagctc ttagctcatc taattgttct tcagttatat
     7981 tgattcctaa ctcatgttca ccttctgcaa gtgcaaccca taaccttctc catgtagaga
     8041 atttaaattg tggagaaaat atataactca tgtccttgct acaatatctg tctgtaagtg
     8101 ggtttgagta agtagtatat ttattatcta tcattatatt agcaccttcc ttaaatcttt
     8161 tgtcgtgtga ttactgtgta cacattagta cataaataat tatatataat tttacgaact
     8221 ataacaagac ttttttattt tttgttcaca tctatatata gtatttttta tttattacta
     8281 ttttttattt atatccaaat tatctttttc aattaagtaa tccactattt cactatcaac
     8341 aaaatttaat acatacttat atctaattgc atagtattta tcactccatc caagctgatt
     8401 ttttacatca ctttcatctg ccccaagcct taaacttatt gctgcaaatg aatgtctaaa
     8461 atctcttgct gaatattgag aaagtccagc taaatctaat gcttttttga ctataagtct
     8521 gacatttcta tctgttatag aattactctt ttgagttgta aaaataaaat catcactagg
     8581 tattattacc tctggaagcc cagaataatt cctgtaatct tctaaaagtt taaaaaagta
     8641 tggatgtaat ttaactactc tttcttttct tcccttacct aaccttacat atgagttatc
     8701 gttttcatct accataaggt ctttccactt taagctaact aattcattta ataaacaccc
     8761 agtagtaact agacagacta ttattactct atctcttata tttaattttg gtaggatacc
     8821 tataagttta tttatttctt gtatacttag aacatcatct tttgttttta ttcttgttac
     8881 tgtgggtttt tctacatatc taaatggatt tatatctatg tatccttctt tttttaagaa
     8941 gttataaaaa ctatgtaaat aactatatat cttttcacaa gttgatttcg catatttagt
     9001 ctgtatatag tctacaaaat ttttacaatc ttcttttgta gcataaatca attctttccc
     9061 ttttaaaaat tctttaaaca gtataacctt tgataaataa tcgtgtttcg tgtttggatt
     9121 taattttgta gaaaaaacat taaaagtttc atactcataa tcatctagta tttttgtact
     9181 atacataata actcctttcc ttttcataaa ataaaatagc tatactttta ctataccata
     9241 tatttccccc tcaaatatac cccttattca atagataact tttaagttta ttttgttata
     9301 ttatgaaggt tttatgaaaa tatcaatctt ccattgtgca ctacacagta ctatggtatt
     9361 ataagttagt aaaaggtggc atttttttat taatctatga ataaaatatg ctactataaa
     9421 tttgtattgg aggtattttg atttggaaac atttagtttg cttgaaattt ttaaggcagt
     9481 cattttgggc atagttcagg gaataacaga atggctacca gtaagtagta cagggcatat
     9541 gatattaatt aatgagttta taaaattgaa tttttcaaat acatttataa atacattttt
     9601 agttgtaatt caatttggtt caatactagc tgtacttgtt atatttttta gaaagttaaa
     9661 tccttttgat agtgcaaaga atattaaaca gaaaaaagaa acagttcgtt tatggcttaa
     9721 ggttataatt gcagttatac catctggagt tattggaata ctgtttgaag atgacataga
     9781 cagagtattc tttaattcaa cagtagttgc tattgctctt attgtctatg gtataataat
     9841 gatagggctt gaaaagagaa ataaaagacc taagtataaa gatttttctc aagttacata
     9901 taaattagct ctttgtatag gattattcca gtgtctagct ctcataccag gtacatctag
     9961 gtctggttct actataatag gtgcagtatt acttggtact tctagatatg tagcagcaga
    10021 gttttcattc ttcttagcca ttcctacaat gcttggagct agtgcattaa aacttctaaa
    10081 agctggattt ggatttactg gatttgaatg gcttatttta ggtgttggtt ctgtagtagc
    10141 atttgttgtt tcaattgtag ttattaaatt ttttatggac tatatcaaaa aacatgactt
    10201 caaagtattt ggttattata gaatagtctt gggtatagtg gttcttgctt atttctttct
    10261 attataatgt attctaattt aataacaaat gataaggaga aaagttatga gtgttaaaaa
    10321 agaaatattt gattggatta agtcaatagc tatggctatt gtacttgcat ttgtaattct
    10381 acaatttata agaccttctg ttgtaagtgg agaatcaatg tatcctactt tagatgataa
    10441 agattatctg attttaaata ggatatcata caaggttggt aaacctgaaa aaggcgatat
    10501 tgtagttttt aaaaccaatt tagttgatgg agaaacagga aagaaaaaag acttaataaa
    10561 aagagttata gctactgaag gtgacagaat aaaaatatca aattctaaag tgtatgtaaa
    10621 tggaaaatta ttaaatgaac catatataca caataactat acttctggag atatagatac
    10681 tgttgttcca aaaggtaaac tatttgcaat gggagataat agagaaaata gtaatgatag
    10741 tagattccct gatgtaggta tggttgatga agatgaaatt cttggtaagg ttatggtgag
    10801 actattacct cttgataata ttgggaaagt ggactaaaaa ataagagact gaattggtaa
    10861 agtttatttt acaaatctca gtctcttttt attttcttaa attacaaata tttttttact
    10921 aaatatcttt tgattctgta ataagtctta ttatatcact tgcttcttca ttaaaagttg
    10981 ttactatact taggtagtct gttgctctac tcatagctaa ataaacctta tttaaatctc
    11041 ctacaaagtc atttacttga taatcttgtt caatatcatt tatagtttga ttgtataaca
    11101 tctctaattc acatactatt accgctttat actctaaatt ctttattgca tatatatttg
    11161 ctattgtagt tcctaccttc ttacttatat ttgtcaaatt atcttctgca catatataag
    11221 gaatttttgc ctcttccaat gcttttttta acatatattg aaagtaaatg gtttttccac
    11281 tttttaattt cttcttatta taaggataaa cgatagcaac ttcagaataa tcaagacctt
    11341 ttttacttat taaatactca atttcccaga ttacagaact tatttggtca tcaaggtcac
    11401 ttacttttat tatatcaaca gacttatttc cacctctaag tgctttagta taaaagaaag
    11461 tactaaattc tgtatttggt cttaattcgt tgatatatga gttagaatta tttgaaaatt
    11521 tatttgtaaa atctacaatc tctttagctt gtctataatt tttacttaag ataatcttat
    11581 catcaaattc tataccatta tataattttt taaatatatt caatctatta gagatattca
    11641 aggagttaca agaacacaca ttaaatatga attttgtttt atataaaaac tcccttataa
    11701 aatctatttc atactctaaa aagctttctg cttcatcaat aaaaatagcc ttgaacatat
    11761 ttttattttt tataatgttc tgtgcttgtt ttacaagatt attaaaggtc ttttcgtaat
    11821 cattttttaa catgttataa tctacaatta aattgaattt tttagctagt ttaaatataa
    11881 aagaactaaa tgtgtgaact tctaaattgt tattatcttt gtataataac tctatcctct
    11941 ctcgaagctc attgcacaat tgttttgtat gtgtaaatat gactaattta tgatgtggat
    12001 atacttttgc taattttata gccctagaaa gcataattgc tgttttgcct ataccagaac
    12061 caccttcaat aagtgtattc ccatatttaa tagaaacaac atctttaatt tgaacgtctt
    12121 ccatcatagt tgctgtatac ttatagtcat cattgtagaa tgaaattttt ttaaattttt
    12181 catttagatg aagtttatca tttattatat agtattctga acatatgtct agtaaaaata
    12241 aatttaagtt tatttcatca ttctcatcat taagatactc ctccagcgaa cccttattca
    12301 ttatatcttg aagcttattt ttatctatta tattattatt aacaaattct tcaaattcat
    12361 atgtttcctc aacttcaacg tatggcataa tgtaaacgta gttataactt atatttctat
    12421 tcttttgtcc catttttagt ttcaaaagtt catattcctc attcataact tcaagtaact
    12481 cttcttctaa aataaaaaat atatcttctg ttgtatccat aaattttata aataaaattc
    12541 ttccatcttt aacatacaac aaatcggtat ttattccttt aaaaggagta acttttggca
    12601 ctactttaac gtcctcattg atattttcgt aaaatatttt ctcgaactcc ttcaatttta
    12661 gggcgtctaa ctgagaattt atatttatgt attggctcac ttacattacc tccttctgtt
    12721 atacttaatt ataccatttt gaactatatt tttatatata ctttttatag gcaaatttag
    12781 taaataaatt caaaataaat acaaataaat acaaaactgc taaaataatt ttgcaataaa
    12841 tatagatatt tattgttata gaatactttc tgtaaataat aaaatgataa atttcataat
    12901 taatgtttaa aatagtaagt aaagttttaa aaaaaataaa tcttgttgac taaaaataag
    12961 atttattata aaaaatttaa aaacctgcta aaaataatct attttcatta gcaggtttta
    13021 tttataatct tcttatatta atactattat atcctatttt tagattttac tgtagtattt
    13081 actatttatt ttgtagtaaa gtattgattt tacaacttca ttatatcaaa caaacttgga
    13141 taattatata aataatacat atataaagct ttattttatt aatcttttgt aagtagcact
    13201 tttagcactt agattatttt tgcattatat aaaattaaac ttaaacaatt taaaagccac
    13261 aagatatatt atcatatctt gtggcaaatt taaatttcta aaaatatttt cttattattt
    13321 agatattatt tcttttactt cgcatactct atagctcttg tttctcttat tatgcttact
    13381 tttatttgtc caggatattc aagttcatcc tctattttct tagtcatatc tctagccaat
    13441 agatgtattt cttcatcatt tatagcttct ggttttacca ttattcttat ttctctacca
    13501 gcttgtatag caaatgattt ttctacacct tcatatgaat tagctatttc ttctaatttt
    13561 tcaagtctct ttatatatgc ttctaaagtt tctcttctcg caccaggtct agcagctgat
    13621 attgcatctg ctgctgtaac taatacagcc tctactgttt gaggttcgta atctccatga
    13681 tgagtactca tggcatgtat aacttcttta gactctttat aacgtctaag taaatccata
    13741 cctatttcaa catgtgtacc ttccatttca tggtctacag ccttacctat atcatgtaat
    13801 aaacctgccc tcttagccaa tctaatatct gctcctattt cagcagccat tataccagct
    13861 atatgagcaa cttctataga atgtttaagt acattttgtc cataactagt tctatagtta
    13921 agtctaccta ataatcttat taattccgga tgaatcccat gtacactagt ttcaaatgca
    13981 gcttgttcac catattcttt tataatattg tcaacttctt ttctcgcctt ttcaaccatt
    14041 tcttcaattc tagctggatg tattcttcca tctgctatta atttttctag agcaattctt
    14101 gcaatttctc ttcttattgg atcaaatcct gataaaatta cggcttctgg agtgtcatct
    14161 attataaggt ctatacctgt taaggtttct aaagtcctta tatttctacc ttccctacct
    14221 attattctac ctttcatttc atcattaggc aagcttacta ctgttacagt agtttcggct
    14281 acgtgatctg ctgcacattt ttgaattgca tatcctataa tttctcttga tttcttatca
    14341 gcttcttctt tagcttgact ttctatctct tttatcatta tagacatttc acgtcttaca
    14401 tctctctccg cattggttaa gatgatttct ttggccttat cagaagtaat tcctgatata
    14461 ctttctaatt tttctaactg ttgagttttt attacttcga cttcttcttc tttcttttcc
    14521 actaccttta atttttcact taggtttact tctttagatt ctaaattttg taattttcta
    14581 tctaagactt cttctttttg tactactctt ttttcatact tttgtacttc tgttcttctt
    14641 tctctgtttt ctctttcagc ttctgttctc cacttatgga tctcttcttt agcttctagt
    14701 aatttttctt tttgtactgt ttctgagtcg tggtgtgctt tatctattat ttctttggcc
    14761 aagttttcag cttgtccaat ttttgcttca gatatatttt ttctaacaaa atatccagct
    14821 ataataccaa cagctgcacc tattacaatc attactacac tatctatgac atccacctcc
    14881 ttcgttaata atcccaaaat gtcatcacta tgattaaaaa atattcctaa taattaaaat
    14941 taaatttaga tattttatcc tcacttaatt ttataatttt ttcaacattg tgtcaagcta
    15001 atagggagcg tggcatgtct ttaattacta gttatttttt tgtattttta cttattcttc
    15061 tacaatttct tttgatactg aagtacctga ttcttcatgt tcttcattta aattgtaaaa
    15121 tgctctaact ttctcatcta tttcagtagt taaatctaaa ttatcttcca agaatttttt
    15181 aacattttct cttccttgtc caagtttagt atcattgtaa ctataccatg aacctgattt
    15241 tttaactata tctacatcag cagctatatc taaaaggtct ccaatttttg aaatcccttc
    15301 tccatacatt atatcaaatt cagcttgctt aaatggtggt gctactttgt ttttaacaac
    15361 tttaactcta gttctactac ctataacttt atcaccttgt tttattgtat ctatttttct
    15421 aacatccaat ctaactgatg aatagaattt tagtgcacgt cctccagtag tagtttctgg
    15481 gtttccaaac attattccta ctttctctct taactggttt ataaatatag caacacaatt
    15541 tgatttttta attgaaccag ttaactttct aagtgcttgt gacataagtc tagcttgtaa
    15601 acctacatga gaatcaccca tatctccatc tatttcagcc tttggaacta atgctgctac
    15661 tgagtctatt actattatat ctatcgctcc acttcttatc aatgcctctg ctatctctaa
    15721 agcctgttca cctgtatctg gttgagatat tattaggtta tcaacatcca cacccaaagc
    15781 ttttgcatat actgggtcaa gtgcatgttc tgcatctata aatgcagcta ttcctccatc
    15841 tttttgagct gatgctacac aactaagcgc aacagtagtc ttaccagaag attctggacc
    15901 atatacttca actattctcc ctctaggtag acctcctata ccaatagcta tgtctaaacc
    15961 aatcgctcct gttgatataa catctataga catagatgtt gcttctccca atttcattac
    16021 tgaaccttta ccaaaatctt tttcaatttt acctaaagct tcattcaacg cttttaattt
    16081 ttcttgatct acactcatct ctataccatc ccttcattta tctacaaaca tttgttctct
    16141 tgttatatat taattatata acataaatca aacctagtca atttaaaaac gctactaatg
    16201 attatatttt tattcattac attttattta taaaccatta ttaataatta tatacacaaa
    16261 agaaaatatt cttaaaatg
//
LOCUS       DAEPOG010000084        16138 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEPOG010000084 DAEPOG010000000
VERSION     DAEPOG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.084"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(44..2323)
                     /locus_tag="KR432_002202"
     CDS             complement(44..2323)
                     /locus_tag="KR432_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0640733.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2594..4048)
                     /locus_tag="KR432_002203"
     CDS             complement(2594..4048)
                     /locus_tag="KR432_002203"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH0640734.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4212..5081
                     /locus_tag="KR432_002204"
     CDS             4212..5081
                     /locus_tag="KR432_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0640735.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5295..7367)
                     /locus_tag="KR432_002205"
     CDS             complement(5295..7367)
                     /locus_tag="KR432_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640736.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
     gene            complement(7670..8512)
                     /locus_tag="KR432_002206"
     CDS             complement(7670..8512)
                     /locus_tag="KR432_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640737.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8759..9253)
                     /locus_tag="KR432_002207"
     CDS             complement(8759..9253)
                     /locus_tag="KR432_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0640738.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(9405..10376)
                     /locus_tag="KR432_002208"
     CDS             complement(9405..10376)
                     /locus_tag="KR432_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH0640739.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(10377..11354)
                     /locus_tag="KR432_002209"
     CDS             complement(10377..11354)
                     /locus_tag="KR432_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640740.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            11573..13132
                     /locus_tag="KR432_002210"
     CDS             11573..13132
                     /locus_tag="KR432_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640741.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            13238..14197
                     /locus_tag="KR432_002211"
     CDS             13238..14197
                     /locus_tag="KR432_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640742.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            14200..15012
                     /locus_tag="KR432_002212"
     CDS             14200..15012
                     /locus_tag="KR432_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640743.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(15227..16129)
                     /locus_tag="KR432_002213"
     CDS             complement(15227..16129)
                     /locus_tag="KR432_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0640744.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
BASE COUNT         5545 a         2530 c         1879 g         6184 t
ORIGIN      
        1 ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt gtttcttttc
       61 tatttaaact atatctatct aatattaatt tttcatattc ttctattggc attggtctag
      121 caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct gcttgctcta
      181 cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt tgaacaatac
      241 ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat tgcctatcta
      301 atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa ccagaaccaa
      361 aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt ttaaataata
      421 tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa tttggttcaa
      481 tttcatattc ttttgttatt ctttcaagct cagaaataaa acctggattc tcaagatgta
      541 ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc caacgtttaa
      601 aatttttaca aacttgggca aatacaaaca tatccaattc tgttataaaa ccatttttct
      661 caaaaatagg aataaataca cctggactta ttagaccttt tttgggatgt tgccaacgta
      721 caagcgcttc agcacctaca attttagtag ttgataaatc aaccttaggc tgtaaataaa
      781 ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca cgttcttcta
      841 tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat tttcttttta
      901 ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt tctccctctt
      961 caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa cttgttttta
     1021 tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct ctattatttt
     1081 tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca gattgctctt
     1141 taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct tcttcatacc
     1201 caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa actagattct
     1261 ttttctcatg agaggataaa aattttcctc cttcaagatt aaatttattt gaattgccaa
     1321 tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc tttttatgta
     1381 taaatagtat ataaattgtt aatgccgtaa agattataat caacactaca catgctgata
     1441 atgtaagttt tataatagcg tatgatttct cagatatagc agttttagga acttcagaaa
     1501 aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct tttccaaaca
     1561 tactataact taatgaacct gatttaaagt tttttatatt tgacttcata ttattgatac
     1621 tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct ttagtagagt
     1681 tggcgttttt gctgcttgga tttaaaacaa tattaccatt acttctagct atataagaat
     1741 atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc gtactatgtg
     1801 tggcaaatat aactccttta acctcattat ctttataaat tggcacagaa tatacattaa
     1861 tattactatt ggtattttta tcatcttcta ttacatcttt taatttttct gttataacca
     1921 cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct atataaccat
     1981 ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat gaactattaa
     2041 tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct tctccctcaa
     2101 taaatattga aattgactct agtaacttta tatctccatt aatttgtttt tttaacacat
     2161 tgacgctttg atttgctatt tctgaaagat attgattcgt ttcttcacct aatgtcttat
     2221 tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt atgacagcaa
     2281 ttatggacaa accataccta ttattttttg catcacttaa cattatgctt cctgcctttc
     2341 tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat tttataataa
     2401 tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt acaacaataa
     2461 attaattaat atagatatta gggtttatat tattgcatta tctattttac aatcatttac
     2521 atataaaaaa atgtaatatc aaagtgaaac ttgatattac atttttgttc ttgtattttg
     2581 tttaattttt atgctacatt ttacttaaac actcatttgc aacttcttta tcatcaaaat
     2641 gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt ataattacaa
     2701 catcatcctt ttttgccata cttatagctt ctgctatagc ttgttctcta tcaacaacaa
     2761 catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt attgcctctg
     2821 gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat ttttgtccaa
     2881 ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacatcca aacacagaaa
     2941 ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt gcatcaggag
     3001 tatgagcata gtctacaatt acacttatac ctttatcatt tggaactgtt tcaaaccttc
     3061 ctgatactcc attagaaagt ctaagacctt ctttatatat tggtttaggt atgccaagta
     3121 catagcaagc agctataact gctaaagtat tatatactgt aaacattcct ggaactggta
     3181 taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca tccgaatctg
     3241 attttatatc tctagccata aaatcagcct tagtatcaac accataagtg taacaaggta
     3301 catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct atgtttatta
     3361 tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa tcctctaaat
     3421 ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt ttaaagtctg
     3481 tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca cagtagtcac
     3541 atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct ggagttgttg
     3601 aattggaaac aatgtctttt tctccgtcaa aaatttttat agttcctata agtccaactt
     3661 tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt tttccattag
     3721 tacctgtaac tcctataacg ttaaatttct gagatggatg attataaaaa ttatcagcta
     3781 cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct tttatttcta
     3841 catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt atatacttat
     3901 gaccatctga cacaaatcct tttatacata taaataatgt accttttgta acctttcttg
     3961 aattatattg tacattatta atatctatat ttaattcacc cttgacattg attatatcca
     4021 acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac tctttctgct
     4081 aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat ggttaaaaat
     4141 gcttgaaata ttgcaaaaaa tgctctataa ttttctataa tataactgag gaaaggtctt
     4201 ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa atatttaata
     4261 ttagagataa aaaagatata caatttgaat accataatca tgattttaat aagataatta
     4321 tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa ctaaagcctt
     4381 gggatatact ctttatcagc aataatgaaa tccataaacc agttatagat tcaaatgtct
     4441 tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat agtgatgcta
     4501 ctagtgactt agaaaaatgt tttaaaatag ctacggagaa taaatgcaat agattgcgtt
     4561 taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat atttccgaat
     4621 ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa cttatgatat
     4681 tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagac gttttttatg
     4741 atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat aagctttcaa
     4801 tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga aaatttaaat
     4861 ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt ttagcaaagt
     4921 cactaataac caatggttac cttatgagtg aagtttgtaa taaatgtgga ttcaacgatt
     4981 attctagttt tgtaagggca tttaaaaaaa actacggtgt atctcctaaa aattatataa
     5041 acaataaaaa tcctttagat attactcttt tagatgaata gataatttat gtaaatttta
     5101 ttttaataaa aacttgaagt attagtgtat ttatcttact aatacctcaa gttttttaat
     5161 gttttaaaat atatcaaaat ataatgatat atatttctat tacttaaaat atatattaaa
     5221 taaattgcat gttaactgaa cctatttata tatttattaa atctcacata acttttagtt
     5281 atactatcat atagctatcc tattttttca atatcatatc ttaatatact tactctgttc
     5341 tcctcttttg ctgtagttat tcctttttta atgcttaatt tctgattttg ttctaaattc
     5401 gtaaatacta caggacttat tgttgataga caattttttt caatatattc taagtccatt
     5461 ttaactaatt tttctcctgc ttttacttca tctcctacat tcacaaatac ttcaaatcca
     5521 tttccttcta aatttacagt ttctatccct aaatgtataa gtatttctac tccacttttt
     5581 ttggatttta ttattattgc atgcttagtt ggaaatataa ctccaatagt tccatcaact
     5641 ggtgaataaa tcaatccatc actagattta attgcaaatc catcccccat cagtttactt
     5701 gaaaatacag aatctggcac ttcttctata tctaatatct ctccatttgt taatgaatca
     5761 atcttttcta ctaaagcatt ttccatattg gcaacttcca tatttttttt cactttattt
     5821 ttatcaacat ttttagtaac agtactttca acctttgttt ttccctctat tatatctttt
     5881 atatcagact ttattgaatc cgattttggt ccaaatatag cttgaacatt gttgccaata
     5941 accataacac ctgctgctcc aagtaatttc aatttttctt tatctacttt atttatatca
     6001 tttacaacca atctaagtct tgttatacac gcatctaggc tctttaagtt gtctttattt
     6061 cctaaagcaa ttaaaatctc tgatgctaat tcattcttag acatttcaat gacttcagcg
     6121 ttagtttctg actcatcttc tcttcctggt gtctttaaat taaattttct tatcataaat
     6181 ctaaatacac tatagtataa tactgcaaaa ccaataccta ccaagatagc caagtaccat
     6241 ggagttctat taggaagaac accaaataat aagaaatcta ttaatcctcc tgaaaatgtg
     6301 aatccaattt taactcctaa taattgcata attgcgtatg ataaacctga aaataaacaa
     6361 tgtattgcaa ataatactgg tgcaacaaat aaaaacgcaa actctattgg ctcggttatt
     6421 cctgttaaaa aagaggttaa tgctgctgat gccattatac ctgctacaaa ttttttcttt
     6481 tctggctttg cttcatgcac catagcaaga gctgctgctg gaagaccaaa catcataaat
     6541 ggaaatgcac ctgtcataaa agtacctgct gtgaaattta ctccatcttt aagttgagca
     6601 aagaatatct tttggtcacc cattacaagc tcaccagctt tatttacata ttctccaaat
     6661 tgataccaaa atggattatg gaaaacatga tgcaaaccaa atggtattaa tagtctttcc
     6721 aaagaacctt ccataaatgc tgctagtgtt tgatttgctt ctataacatt ttttgagaaa
     6781 ctaagtaatc cctcttgtat tggaggccaa ataaaagaca atataattcc tattgctata
     6841 gcaactaatg aagtaacgat tggtacaaat cttttacctg caaaaaaacc tagatactga
     6901 ggtaattcaa ttttataaaa ctttttataa agataagcag ccactatccc tataataata
     6961 ccaccaaata cccctgtttg aattgttggt attccaacaa tacttgcata catagaattg
     7021 tctaccatat cttctgtaac acccgccata acacccatag atgtattgag aattaaaaaa
     7081 cctactatgg ctgccaatgc tgcaacacct tctccttcag ttaaaccaac agctacccca
     7141 actgcaaata taagtggtaa gttattaaat ataatttgac ctgaactttc cattatactt
     7201 attataagtt gaaatgtagg attcgccaaa gctggaaaat gagacaacaa tgcgtctcct
     7261 gctaaagcat tactcacgcc aagtaatata cctgctgcag gaagtaatgc aactggtagc
     7321 attagagatt ttcctacttt ttgtaaaaca ccaaatactt ttttcattta cttttctctc
     7381 cttattattt tatatttttt taaataaaaa agacttaagt ttataaaaag ttacattagt
     7441 atacttaatt ctaaaattaa acttatgtta atataaataa acctatatta acctaatttc
     7501 agaattaaaa ataaactatt ataatttttt tataaacata agtcttgcct gcattatcag
     7561 taacaatctt aatgttttaa aattattatt ttatattaaa ttttaaataa tatattaaaa
     7621 ttaaacataa tatattaaac ctatacattc tgtattaaac tactctttat tataaattca
     7681 gtacagatat aactcttttg atatgtaatg ttaaataaag tagttcatct tgacttacat
     7741 agtaatcata attcttttct acatgagttt ttattttata tgcacaatca taatcctttt
     7801 catactttac tttaattata tctataattt cattatttgt atcattgatt ttttcttttt
     7861 ttactaatcg ttttgcaaag aattttaaat gagtcaaaag cctatcataa tttatatcat
     7921 cttctttaaa ttcaactcta taataatatc ttataatatt tagtatgtct tttacaattt
     7981 tagtaattaa cagtgattcc tttgaacttc ccttatagtt tgaatttact atatgcattg
     8041 ctataaaacc agcttcatct actggaaatt tcacatcaaa ttctttattt atataatcta
     8101 cagcccattt accaatctca tattcttttt tatgtattct ttttatctca tgtagtaaat
     8161 cattctttat agttatattt tcatgatatc ttttaattgc aaatgcaata tggtcagtaa
     8221 gtgctacata aatatattca taaagcttag tattcaaatt ctcctctgca taagcaatta
     8281 tttcagtact aatctcaaaa aatttctcat caacctggct tgctagtttg ctaattttat
     8341 ctaaaaagct ttcatcttgt attacaaaag ttctttcaat tttactttta tctatttctt
     8401 gacctttctt tttttgaaac ccaatcccac atcctgttag tattaattct ttttttatat
     8461 tttcatctaa aactaataca acattattat tataaatttg ttgaataatc ataatcaata
     8521 tctctcccaa aatacaattt cccttattca taaaaaaata cctaaattaa gatacaattc
     8581 acctgtatac cttaacctag gtattgcctg cttaccagta acaatccagc ttgttaattc
     8641 ttatatattg tattcaattt atggttttat tctattataa ataaatatat atgtcaagta
     8701 aattattgaa aactatttcc tattttttag tattattttt aacatcaaaa atcaaatttt
     8761 attccattat atttatgatt ctatcatcat catttaataa agattcacct tcaagtatac
     8821 tcatcatatt tttaggaaaa tttaatcctt gtgtgattga ttttactgct actaaatcag
     8881 ttgaagatat tactgctgcc aaagcaagtg aaacagataa tggcatccta ggaataagaa
     8941 aatgtataaa gattccacct gcaaacacgg ttatcaaaac taaaccaaat gcaagaagca
     9001 aaattggttt tctaagctct agtaactctt ttcttgatac attttgtcct tcactgaata
     9061 ataatggagc tatgatacat attacaaata tttctggatt aaatgaaaat gataatttaa
     9121 acggcaaaat agacaatata atccctagta caatttgata tattgcaaga ggaatattac
     9181 tccacttttt gctaaataca gttgaaatct ttgtacaaac caataatccc aacaatgtta
     9241 ctaaagttga cataactacc tctcttttca ataataatat tataaattaa acttatatat
     9301 caactaactt aatattaaca acatgaaatc ccatcttaat caaaaaaatt ttcagtttat
     9361 tctattattc ttattatata catcaaattt aatttttgaa atatctatac tagatggcat
     9421 gcaacaaaat gatttcttga tatttctttt aaaactggaa tctctttttt gcatatttcc
     9481 ttacaataag ggcatcttgt atgaaactta catccacttg gtggatttgt tggagatggt
     9541 atgtctcctt ttaatataat tctatttttc ttaattgtag ggtcttgtac tggtactgcg
     9601 ctcataagtg ctcttgtata tggatgttga gaatgtaagt aaagttctcc tttagttgca
     9661 atttctatta tagaacctag atacataaca caaactctat gtgcaatatg atttactaca
     9721 ctcaaatcat gagatataaa taaatatgag agtttatatt tatcctgtaa atccataagt
     9781 aaatttataa tttgagattg aatagaaaca tctagtgcag aaacaggttc atcacaaact
     9841 ataaattctg gatttaatgc taatgctctt gctataccta tcctctgtct ttgacctcca
     9901 gaaaactcat ctggttttct atttaaatgg tacttagaaa gaccacaact ttctacaaca
     9961 tttaatatat tatcttttat ttcattcttt ggcactaaat tatgttctat aactgcctct
    10021 gatattattt gttccacatt taatcttgga tttaatgaac tatatgggtc ttgaaatatc
    10081 atttgcattt tgggtcttaa ctttctaagt tcttccttct ctaacttata tatatctaca
    10141 ccattaaatt caactttacc ttcagttttt tcaattaatc taaggatggt tcttcctgca
    10201 gttgattttc cacatcccga ttctccaact atagccaaag tttctccttt ttttaaatca
    10261 aaagaaattc catctactgc ctttacacat ttaatttcct tatcaaataa tccagattta
    10321 acttcaaagt actttttaag accttctact tttaataaac tctcttcttt tttcaattat
    10381 atctcctcct ttttatataa ccaacatgca atactatgac cactattcaa ttctattaaa
    10441 ggaggtatct gagttctaca tttatcacat actttttcac atctatcact aaaataacat
    10501 gaatctggta ttccaattgg atttggtact tgtcctggaa tcgaatacaa tcttttatct
    10561 ttgttttttc caagaacagg ctttgatttt agaagtccaa tagtataagg atgttttgga
    10621 tttttaaata attcaacaac ctctgccact tctataattt taccagcata catcactaat
    10681 actttgtcac acatttctgc aactactccc aaatcatgtg tgatcatcat tatagatgtg
    10741 tttaattttt cttttatatt tttcattata tctaaaatct gagcttgaat agtaacatct
    10801 aaagctgttg ttggttcatc agctataagt aattttggat tacatgataa agccatagct
    10861 atcattattc gttgtctcat accacctgat aattcatgtg gatatgaatt atatatttct
    10921 tcagctcttg gtatttctac cagttttatc atatcgatta cctttttctt agcttcttcc
    10981 tttgataaat cttgatgtat cataatacct tcaatgattt gacttccaac agtgaacgct
    11041 gggtttaaag atgtcattgg ttcttgaaaa atcattgata tctcattccc tcttattacc
    11101 ataagttcat cttcattcat ctttaaaatg tctttgcctc gaaagattat ttctccacct
    11161 tctatttttg atgttggaag taacttcatc aaagacatgg ctgttacaga cttaccacat
    11221 cctgattctc caactattcc aatagtttca ccttcattta tattaaaact tacttcattt
    11281 acactcttta caatcccttt atctgtatga aaataaactt ttaaatcttt aacctcaatt
    11341 agtgctttac tcattatttt ctcctctcag caaaattact taaaataaca tacacctttt
    11401 gattgtattt tataaaaatt taaattacaa cattttctta cattttatta tttttttcta
    11461 ttaattataa taatataata gttaatttct atatatattg atttaataat gattactatt
    11521 atacaatgaa aaatatgtgt taaatattat tttatggagg ttttataaga atatgaaatt
    11581 taaaaaattg gcatcattaa tcttagtttc atcattaatg cttacattta cagcctgtgc
    11641 ttccaatgat aaagacaagt ctagtggaag caaatctggt ggaactgttg taatacctat
    11701 ggcaagtgac ccagatataa tcaatggagc ttttgcaaac gttaaagaag actctcttgc
    11761 atcaaatata gtttatgctc ctctttatac ttatgaaaaa gggaatttag taaattattt
    11821 agcagaaaaa gtagatttta aagatagtaa ggaattaact ataaaattaa aatccaattt
    11881 aaaatggcat gatggaaaac caataactgc tgaagatgtc ttatttacat ttaatacagt
    11941 tttagacgaa aaacaaaatt caccatcaag acaatactta ttggtgggtg aaaaaccagt
    12001 taaagtagaa aaaattgatg atttaacagt taagattact ttaccaactg caagtgaatc
    12061 tttcttatat ggtatatcta aaatttctcc aataccaaaa catgtatttg aaggagaatc
    12121 aaatatagct aagtcagaaa aaaataataa tccagttggt tctggtgcat tcaagtttaa
    12181 ggaatggaaa aaaggtgaaa gcatagtatt tgaaaagaat gcagattatt ttggtggaga
    12241 acctaaggca gattctatag cgctaaaaat aattcctaat gaagcatctc aagaagcagc
    12301 tttaaataat ggcgaaattt cacttatgaa gacatctgct gaaggatatg aaaaggcaaa
    12361 atcaaactca aacttacaaa cttatactta tagtgaagaa agattaaact acattgtatt
    12421 taaccaaaat atatcaaaca tggctaacaa agaagtaaga caagccttat catatgcctt
    12481 aaatagaaat gaaatgatag aatcagcata tggaaaagaa ggttctgtac ctgctaaatc
    12541 tatattagtt ccagaagcag acttctatac agaagaaggt gttgaaggat atgaccaaga
    12601 tacaaataag gctaaagact tattggataa aagtggtgtt aaaattgata aattaaaaat
    12661 aggttataac acaggtagat ttgggcataa aaactatgcc ttagtagctc aacaagagtt
    12721 gaaaaaaatt ggtatagaag ctgaaatagt tccttatgaa agtaaggcat tctttaatat
    12781 actatttagt aatagcactg aatgtgatat gtatgtaaat ggttatgctt ggggattaga
    12841 accaaaccca tatagaggta tgtttgaaac tggtcaatat tgcaaccaaa ctaaatattc
    12901 taatgctgaa atagatgctt tatgggaaaa aggatttact gagttaaaca aggaaaaacg
    12961 taaagaaata tacaaacaaa tacaacagga tatatctaag gatgcaccta tttacactat
    13021 agattatgaa caaaatttaa tggcagctca aaaaaatcta aaaggaataa aagatgctaa
    13081 accatctcct gcaatattat ttgaagattg gtctaaactg tatgtagaat aattgatgta
    13141 aaattttata ttattaatta gaaaaggaga tgtctaaaaa taatattgtt ttattatttt
    13201 aaaacgagac atctctttta attaggagaa tttacttatg ttaaaattaa tattgaatag
    13261 agtaaaagtt ttaataccta tgttaatctt aatatctata ttatcatttg gacttttaga
    13321 attagctcct ggtgacccag cagatgcata tataaatcct ttaatgtctt cacaggatat
    13381 tgaaaatata agggttaata tggggcttga taaaccagta tatataagat atttaaactg
    13441 gttgaaaaac actttaagtg gaaacttagg tatttcttat ataaaccata tgcccgttac
    13501 agaacaaatt atggaaaaaa tgggaaatac atttattctt atgggtactt ctttgatatt
    13561 ttcaatattg gtagctatac ctcttggaat atttttagct gtaaataaaa attcaataac
    13621 tagtaaagta tcatctgttt ttaattatat aggtgtttct atcccttctt tttggatagg
    13681 tatgatactc atttcaattt tttcagttaa acttaacatt ttcccatcag gaggtatgca
    13741 tactattgga aatgatagta ttggagattt agtaaaacat ttagttttgc ctgtaataac
    13801 tttaggtcta tataatactg ctatttttac aaattatgta gaagctagtg taaatgaaca
    13861 attaaaaaaa caatatgtta ttacagcaag agcaaaagga ttatcagaaa aagtcatact
    13921 atttaaacat gtcttgaaaa attcactgac atcattggtt acaatcctag gcatgagtat
    13981 acaaaaatta gtcactggtg catttgttac agaagtagtt ttctcttggc caggtatggg
    14041 aagattaatg attgattcaa ttttttcaag agattatact gtaattatgg caataactat
    14101 gttatcagca ctatttttaa ttttaggtaa tttagttgct gatatactat acttattaat
    14161 tgaccctaaa ataaaatcta gcaaaggagg attctagttg tgtttacaag tatcttaaaa
    14221 aaattaaaaa aagataaatc ctttgctttt gcatgtatta ctcttagcat aataatcata
    14281 ttaattatag cttcttttat attaaatata gactctaatg caattgatac tacttctaaa
    14341 cttcaagaac ctagcttaaa gcatatcttt ggtacagacg aacttggtag agattacttc
    14401 tctagagctc tttatggtgg aaggatttca cttagtgttg gtattttatc aatgcttatc
    14461 tctgtagtat ttggtactgc tgtaggtgtt attagtggat atattggagg taagctagac
    14521 caattcttga tgagattagt agatatgctt atgagtatac caagtttctt agttttgatt
    14581 gtattaaata cttatctaac acctaaagtt tccactatta tactaattat tggattctta
    14641 agttggatgg aagtttcaag aattgtacgt ggcgaaacat taaaaataaa ggaaaatgaa
    14701 tactgtttag ctgctaaagc tactggtatc aagactagaa atatactatt aagacatata
    14761 gttttaaact taaaagagac tattgtagtc gctggttcgt taaatattgc taatgctatc
    14821 ttaactgaat ccgctcttag ttttttaggt cttggaatcc aattacctct tgcatcatgg
    14881 ggtaatatgc ttcaagatgc tcaaagacat atttttgaca agttatattt agctgtattt
    14941 ccaggattac ttatattttt aacagtcata agtttgacta taataagcaa aaaattttct
    15001 agtaatactt aacaaatttt atatagaaaa taaaaacagt gattatctaa agatatacac
    15061 tgtttttatt tttcgtttaa aaaatacata ttttgtatat tataacttta aatactactc
    15121 taccatgtta tgtaaagcct attattgctc tcaaacatat agatgaaaaa ctaatagttt
    15181 attgtaataa aaattgattt atttatttgt tattttaata acatttttaa gagttctttt
    15241 tactaaagaa atctataata aatttaaata catgttggtc tacatggcta taacgttctt
    15301 ttttaggtct acatagtata actttccatg taataggatg attaaaaggc ttttctataa
    15361 ttttgctatt ttcaaaaaaa tcatgaactg gtgatggtaa aattgttata aaatcagaat
    15421 ttgtggttga tagcaacaaa aaatcccatg aaccagacat tatatatctt tttaacttaa
    15481 cattttcatc agtaaatttt tgcattagct tatgatgtat cataaaagta ggattaaata
    15541 ttgcaagtaa ttgctcattc aaatcgttcc aatctatctt atccttctta gcaagtggat
    15601 gatttatatt taaaaatgct gtaagttcat cttcttgaag cacatgttca gttactacac
    15661 ttttatcaat tttatgtgga tgtaacagta ctgcataatc tagttcctgc aagataagca
    15721 ttctatttaa gtcatatgca cccttctcta ctatatcaaa ttctatatct ggattatttg
    15781 caactaactg tgcaactaca tctgaaaatg ctattccaag tataagtgga ggtattccta
    15841 ttctaacttt tcctttaaat tgaactgaat cttctcttaa atcttccatc atatttttgt
    15901 attcatttat aatatgttct gcatttacat aaaatctttc tcctgctggt gttaacccat
    15961 ttaacctacc tttatacctt tcaaataaat atacatcttc actttcttca aaatttttta
    16021 taatttgact taatgctggt tgagatatgt gaagtgtctt agatgctatt gataaattaa
    16081 aaccagattt cactatttca acaaaatatt tcaactgttt tatatccatt ttaaaccc
//
LOCUS       DAEPOG010000085        15959 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEPOG010000085 DAEPOG010000000
VERSION     DAEPOG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.085"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="KR432_002214"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="KR432_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH0640745.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="KR432_002215"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="KR432_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH0640746.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="KR432_002216"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="KR432_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBH0640747.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="KR432_002217"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="KR432_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH0640748.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="KR432_002218"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="KR432_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH0640749.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="KR432_002219"
     CDS             2645..3151
                     /locus_tag="KR432_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH0640750.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="KR432_002220"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="KR432_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH0640751.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="KR432_002221"
     CDS             3966..4754
                     /locus_tag="KR432_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0640752.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="KR432_002222"
     CDS             5225..8941
                     /locus_tag="KR432_002222"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH0640753.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="KR432_002223"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="KR432_002223"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH0640754.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="KR432_002224"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="KR432_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH0640755.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="KR432_002225"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="KR432_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH0640756.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="KR432_002226"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="KR432_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0640757.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaagat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttataaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAEPOG010000086        15942 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEPOG010000086 DAEPOG010000000
VERSION     DAEPOG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.086"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            85..639
                     /gene="tcdR"
                     /locus_tag="KR432_002227"
     CDS             85..639
                     /gene="tcdR"
                     /locus_tag="KR432_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBH0640758.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            970..8070
                     /gene="tcdB"
                     /locus_tag="KR432_002228"
                     /gene_synonym="toxB"
     CDS             970..8070
                     /gene="tcdB"
                     /locus_tag="KR432_002228"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH0640759.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8192..8692
                     /gene="tcdE"
                     /locus_tag="KR432_002229"
     CDS             8192..8692
                     /gene="tcdE"
                     /locus_tag="KR432_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBH0640760.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8771..8932
                     /locus_tag="KR432_002230"
     CDS             8771..8932
                     /locus_tag="KR432_002230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640761.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9021..9203
                     /locus_tag="KR432_002231"
     CDS             9021..9203
                     /locus_tag="KR432_002231"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640762.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9420..>15942
                     /gene="tcdA"
                     /locus_tag="KR432_002232"
                     /gene_synonym="toxA"
     CDS             9420..>15942
                     /gene="tcdA"
                     /locus_tag="KR432_002232"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0640763.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFA"
BASE COUNT         6435 a         1698 c         2511 g         5298 t
ORIGIN      
        1 tataattata atgactgatt taattccaat gttgtcaaaa tttttaaata aatcatcatt
       61 ataaatataa gagaggatga ttttatgcaa aagtcttttt atgaattaat tgttttagca
      121 agaaataact cagtagatga tttgcaagaa attttattta tgtttaagcc attagtaaaa
      181 aaacttagta gagttttaca ttatgaagag ggagaaacag atttaataat attttttatt
      241 gaattaataa aaaatattaa attaagtagc ttttcagaaa aaagcgatgc tattatagtc
      301 aaatatattc ataaatcatt actgaataag acttttgagt tgtctagaag atattctaaa
      361 atgaagttta attttgtaga atttgatgaa aatatcttaa atatgaaaaa taattatcaa
      421 agtaagtctg tttttgagga agatatttgt tttttcgaat atattttgaa agaattatct
      481 ggtattcaaa gaaaagttat tttttataaa tatttaaaag gatattctga tagagaaata
      541 tcagtgaaat taaaaatatc tagacaagct gttaataagg ctaaaaatag agcatttaaa
      601 aaaataaaaa aagactatga aaattatttt aacttgtaat taatgaattt aaagaaatat
      661 ttacaataga aatcaaattt tagaattaac tttattgtaa aatcaataac ttaatctaag
      721 aatatcttaa tttttatatt ttatatagaa caaagtttac atatttattt cagacaacgt
      781 ctttattcaa tcgaagagca aattaatcaa ctgagtgtct tcaatttaaa atgttaggaa
      841 gtgaatgtat atgaaaacct aagtagatat tagtatattt tataaataga aaggaggata
      901 tataaaagag ttttagcatt tagatgtaaa aatattcaat aaaaatatta tagtaaagga
      961 gaaaatttta tgagtttagt taatagaaaa cagttagaaa aaatggcaaa tgtaagattt
     1021 cgtgttcagg aagatgaata tgtagcaata ttggatgctt tagaagaata tcataatatg
     1081 tcagagaata ctgtagtcga aaaatattta aaattaaaag atataaatag tttaacagat
     1141 atttatatag atacatataa aaagtctggt agaaataaag ccttaaaaaa atttaaggaa
     1201 tacctagtta cagaagtatt agagctaaag aataataatt taactccagt tgagaaaaat
     1261 ttacattttg tttggattgg aggtcaaata aatgacactg ctattaatta tataaatcaa
     1321 tggaaagatg taaatagtga ttataatgtt aatgtttttt atgatagtaa tgcatttttg
     1381 ataaacacat tgaaaaaaac tatagtagaa tcagcaacaa atgatacact tgaatcattt
     1441 agagaaaact taaatgaccc tagatttgac tataataaat tttacagaaa acgtatggag
     1501 ataatctatg ataagcagaa aaatttcata aattactata aaactcaaag agaagaaaat
     1561 cctgacctta taattgatga tattgtaaag atatatcttt caaacgagta ttcaaaggat
     1621 atagatgaac ttaattccta tattgaagag tcattaaata aagttacaga aaatagtggg
     1681 aatgatgtta gaaactttga agaatttaaa ggtggagagt cattcaaatt atatgaacaa
     1741 gagttggtag aaagatggaa tttggcagct gcttctgaca tattaagaat atctgcctta
     1801 aaagaagttg gtggtgtata tttagatgtt gatatgttac caggaataca accagaccta
     1861 tttgagtcta tagagaaacc tagttcagta acagtggatt tttgggaaat ggtaaaatta
     1921 gaagctataa tgaaatacaa agaatacata ccaggatata cttcagagca ttttgatatg
     1981 ttggatgaag aagttcaaag tagttttgaa tctgttctag cttctaagtc agataagtca
     2041 gaaatattct catcacttgg cgatatggag gcatcaccac tagaagttaa aattgcattt
     2101 aatagtaaag gtattataaa tcaaggacta atttctgtga aagactcata ttgtagcaat
     2161 ttaatagtaa aacaaatcga gaacagatat aagatattga ataatagttt aaatccagct
     2221 attagcgagg ataacgattt caatactaca acgaatgctt ttattgatag tataatggct
     2281 gaagctaatg cagataatgg tagatttatg atggaactag gaaagtattt aagagttggt
     2341 ttcttcccag atgttaaaac tactattaac ttaagtggcc ctgaagcata tgcggcagct
     2401 tatcaagatt tattaatgtt taaagaaggt agtatgaata tccatttgat agaagctgac
     2461 ttaagaaact ttgaaatctc taaaactaat atttctcaat caactgaaca agaaatggct
     2521 agcttatggt catttgacga tgcaagagct aaagctcaat ttgaagaata caaaaaaaat
     2581 tactttgaag gttctcttgg agaagatgac aatcttgact tttctcaaaa tacagtagtt
     2641 gacaaggagt atcttttaga aaaaatatct tcattagcaa gaagttcaga gagaggatat
     2701 atacactata ttgttcagtt acaaggagat aaaattagtt atgaagcagc atgtaactta
     2761 tttgcaaaga ctccttatga tagtgtactg tttcagaaaa atatagaaga ttcagaaatt
     2821 gcatattatt ataatcctgg agatggtgaa atacaagaaa tagacaagta taaaattcca
     2881 agtataattt ctgatagacc taagattaaa ttaacattta ttggtcatgg taaagatgaa
     2941 tttaatactg atatatttgc aggtcttgat gtagattcat tatccacaga aatagaaaca
     3001 gcaatagatt tagctaaaga ggatatttct cctaagtcaa tagaaataaa cttactggga
     3061 tgtaacatgt ttagctattc tgtaaatgta gaggagactt atcctgggaa attattactt
     3121 agagttaaag ataaagtatc agaattaatg ccatctataa gtcaagactc tattatagta
     3181 agtgcaaatc aatatgaagt tagaataaat agtgaaggaa gaagagaatt attggatcat
     3241 tctggtgaat ggataaataa agaagaaagt attataaagg atatttcatc aaaagaatat
     3301 atatcattta atcctaaaga aaataaaatt atagtaaaat ctaaaaattt acctgaatta
     3361 tctacattat tacaagaaat tagaaacaat tctaattcaa gtgatattga actagaagaa
     3421 aaagtaatgt tagcagaatg tgagataaat gttatttcaa atatagatac acaagtggta
     3481 gaaggaagga ttgaagaagc taaaagctta acttctgact ctattaatta tataaagaat
     3541 gaatttaaac taatagaatc tatttctgat gcactatacg atttaaaaca acagaatgaa
     3601 ttagaagagt ctcattttat atcttttgag gatatattgg agactgatga aggctttagt
     3661 ataagattta ttgataaaga aactggagaa tctatatttg tagaaactga aaaggcaata
     3721 ttctctgaat atgctaatca tataactgaa gagatttcta agataaaagg tactatattt
     3781 gatactgtaa atggtaagtt agtaaaaaaa gtaaatttag atgctacaca tgaagtgaat
     3841 actttaaatg ctgcattttt tatacaatca ttaatagaat ataatagttc taaagaatct
     3901 cttagtaatt taagtgtagc aatgaaagtt caagtttatg ctcaattatt tagtactggt
     3961 ttaaatacta ttacagatgc agccaaagtt gttgaattag tatcaactgc attagatgaa
     4021 actatagatt tacttcctac attatctgaa ggattacctg taattgcaac tatcatagat
     4081 ggtgtaagtt taggtgcagc aatcaaagag ctaagtgaaa caagtgaccc attattaaga
     4141 caagaaatag aagctaagat aggtataatg gcagtaaatt taacagcagc tacaactgca
     4201 atcattactt catctttagg aatagctagt ggatttagta tacttttagt tcctttagca
     4261 ggaatttcag caggtatacc aagtttagta aacaatgagc ttatcctaag agataaagca
     4321 acaaaagttg tagattattt tagtcatata tcattagctg agtctgaagg agcatttact
     4381 tcattagatg ataaaataat gatgccacaa gatgatttag taatatctga gatagacttt
     4441 aataacaatt caataacttt aggtaaatgt gaaatctgga gaatggaagg tggctcaggt
     4501 catactgtaa ctgatgatat agatcacttc ttttcagcac catcaataac atatagagag
     4561 ccacacttat ctatatatga cgtattggaa gtacaaaaag aagaacttga tttgtcaaaa
     4621 gatttaatgg tattacctaa tgctccaaat agagtatttg cttgggaaac aggatggaca
     4681 ccaggtttaa gaagcttaga aaatgatggc acaaaactgt tagaccgtat aagagataac
     4741 tatgaaggtg agttttattg gagatatttt gcttttatag ctgatgcttt aataacaaca
     4801 ttaaaaccaa gatatgaaga tactaatata agaataaatt tagatagtaa tactagaagc
     4861 tttatagtcc cagtgataac tacagaatat ataagagaga aattatcata ttctttttat
     4921 ggttctggag gaacttatgc attatctctt tctcaataca atatgaatat aaacatagaa
     4981 ttaaatgaaa atgatacttg ggttatagat gtcgacaatg tcgtaagaga tgtcactata
     5041 gaatctgata aaattaaaaa aggagattta atagagaata ttttatctaa attaagtatt
     5101 gaagacaata aaattatttt agataatcat gaaattaatt tctctggaac attaaatgga
     5161 ggtaatggat ttgtatcttt aacattctca atcttagaag gaataaatgc agttatagaa
     5221 gttgatttat tatctaaatc atataaagtt cttatttctg gtgaactaaa aacattgatg
     5281 gcaaattcaa attctgttca acagaaaata gattatatag gattgaacag cgaattacaa
     5341 aaaaatatac cttatagttt tatggatgat aaaggaaaag aaaatggatt tattaattgt
     5401 tctacaaaag aaggtttatt tgtatctgaa ttatctgatg tagttcttat aagtaaagtt
     5461 tatatggaca atagtaaacc tctatttgga tattgtagta atgatttgaa agatgttaaa
     5521 gtcataacta aagatgacgt tattatatta acaggatatt atttaaaaga tgatataaaa
     5581 atctctcttt cttttactat acaagatgaa aatactataa aattaaatgg agtatattta
     5641 gatgaaaatg gagtagctga aatattgaaa tttatgaata aaaaaggtag tacaaatact
     5701 tcagattctt taatgagctt tttagaaagt atgaatataa aaagtatttt cataaattcc
     5761 ttacaatcta atactaagct tatattagat actaatttta taataagtgg tactacttct
     5821 attggtcaat ttgagtttat ttgtgataaa gataataata tacaaccata tttcattaag
     5881 tttaatacac tagaaactaa atatactcta tatgtaggta atagacaaaa tatgatagta
     5941 gaaccaaatt atgatttaga tgattctgga gatatatctt caactgtcat taatttttct
     6001 cagaaatacc tttatggaat agacagttgt gttaataaag ttataatttc gccaaatata
     6061 tatacagatg aaataaacat aacacctata tatgaagcaa ataatactta tccagaagtg
     6121 attgtattag atacaaatta tataagtgaa aaaatcaata ttaatatcaa tgatttatct
     6181 atacgatatg tatggagtaa tgatggaagt gattttattc ttatgtcaac tgatgaagag
     6241 aacaaggtat cacaagttaa aataagattt actaatgttt ttaaaggtaa tactatatca
     6301 gataagatat cttttaattt tagtgataag caagatgtat ctataaataa agttatttca
     6361 acatttacac cttcatatta tgtggaagga ttacttaatt atgatttagg tctgatttct
     6421 ttatacaatg agaaatttta tattaataac tttggaatga tggtgtctgg attagtatat
     6481 attaatgatt cattatatta tttcaagcca ccaataaaga acttgataac tggatttaca
     6541 actataggtg atgataaata ctactttaat ccagataatg gtggagctgc ttcagtcgga
     6601 gaaacaataa ttgatggcaa aaactactac ttcagccaaa atggagtgtt acaaacaggt
     6661 gtatttagta cagaagatgg atttaaatat tttgctccag cagatacact tgatgaaaat
     6721 ctagaggggg aagcaattga ttttactggc aaactaacta ttgatgaaaa tgtttattat
     6781 tttggagata attatagagc agctatagaa tggcaaacat tagatgatga agtgtactat
     6841 tttagtacag atacaggtag agcttttaaa gggctaaatc aaataggtga tgataaattc
     6901 tatttcaact ctgatggtat tatgcaaaaa ggatttgtta atataaatga taagacattc
     6961 tattttgatg attctggtgt gatgaagtca ggatatactg aaatagatgg aaaatatttt
     7021 tactttgctg agaatggaga aatgcaaata ggagtattta atacagcaga tggatttaaa
     7081 tattttgctc atcatgatga agatttagga aatgaagaag gtgaagcact ttcatattct
     7141 ggtatactta attttaacaa taagatttat tattttgatg attcatttac agcagtagtt
     7201 ggatggaagg atttagaaga tggttcaaaa tattactttg atgaagatac agcagaagca
     7261 tatataggta tctcaataat taatgatggt aaatattatt ttaatgattc tggaatcatg
     7321 caaattggat ttgtcacaat aaataatgaa gtattttatt tctctgattc tggaatagta
     7381 gaatctggaa tgcaaaatat agatgataat tatttctata tagacgaaaa tggtctagtt
     7441 caaattggtg tatttgacac ttcagatgga tataaatact ttgcaccagc taatactgta
     7501 aatgataata tctatggaca agcagttgaa tatagtggtt tagttagagt tggtgaagat
     7561 gtatattatt ttggagaaac atatacaatt gagactggtt ggatatatga tatggaaaat
     7621 gaaagtgata aatattattt cgatccagaa actaaaaaag catataaagg tattaatgta
     7681 attgatgata taaaatacta ttttgatgag aatggaataa tgagaacagg tcttataaca
     7741 tttgaagata atcattacta ttttaatgaa gatggtatta tgcaatatgg ttatctaaat
     7801 atagaagata agacgttcta ctttagtgaa gatggtatta tgcagattgg agtatttaat
     7861 acaccagatg gatttaaata ttttgcacat caaaatactt tagatgagaa ttttgaggga
     7921 gaatcaataa actatactgg ttggttagat ttagatgaaa agagatatta ttttacagat
     7981 gaatatattg cagcaactgg ttcagttatt attgatggtg aggagtatta ttttgatcct
     8041 gatacagctc aattagtgat tagtgaatag ataaaaatac gttaaatata tcctcttata
     8101 cttaaatata taaaaataaa caaaatgata cactacataa agtgttctat ctaatatgaa
     8161 gatttaccaa taaaaaggtg aactatgatg aatgcacagt agttcacctt tttatatttc
     8221 taatggtaac aaaatatttt tttatataaa cctaggaggc gttatgaata tgacaatatc
     8281 ttttttatca gagcatatat ttataaagtt agtaatttta actatatcat ttgatacatt
     8341 attaggatgt ttaagtgcaa taaaaagtcg taaatttaat tctagttttg gaatagatgg
     8401 aggaatcaga aaagtagcaa tgatagcatg tatatttttt ttatcagtag ttgacattct
     8461 tacaaagttt aactttttat ttatgttacc acaagattgt atcaattttt taagactaaa
     8521 acatcttgga atatctgaat ttttctctat tttatttatt ttatatgaaa gtgtaagcat
     8581 attaaaaaat atgtgcttat gtggattacc agtacctaag agattaaagg aaaaaatagc
     8641 agttttacta gatgcaatga cagatgaaat gaatgctaag gatgaaaagt aagtaatggt
     8701 agatataata aaaatattaa caaataaaaa gtgttatcca aataagaata gctgaaagtt
     8761 atcataattc atgaaactga taatgaaaac gagggagcag atgccaagag acacacaagt
     8821 attaaataca tataatttcg aagcaagtgt tcattactat atggatgaca aggtagtata
     8881 tcaaacattg gttcacaaag atggtgcatg gtcagttggt aaaatctatt aagctacatt
     8941 agttacagat atcacaaact ataatagtta aacatagaaa tatgtgtaaa ttgtgatgga
     9001 aattattcaa aaacacaaaa atacgtgatg aaggacaaaa tgatagagaa aataagtatc
     9061 aaaccttaat aaatgattta attgatagtt taaaagttat aggaaaaata tataaagaaa
     9121 taaaaacatt aaaaaaatat aagatatgtt tacaaattac tatcagacaa tctccttatc
     9181 taatagaaga gtcaattaac taattgagta tctttaaatt gaaatgttag gaagtgattt
     9241 aaatatgaaa acttaaatta taaaaaatca atattaattt atctttaaaa aatagaaagg
     9301 agtgtataag atttattttc aaagtttaaa aacaagaaaa tcaatttaaa tttcagaagg
     9361 aataaatgtg gttatagaag tggatttatt atcaaaaata atagtactag gaggttttta
     9421 tgtctttaat atctaaagaa gagttaataa aactcgcata tagcattaga ccaagagaaa
     9481 atgagtataa aactatacta actaatttag acgaatataa taagttaact acaaacaata
     9541 atgaaaataa atatttacaa ttaaaaaaac taaatgaatc aattgatgtt tttatgaata
     9601 aatataaaaa ttcaagcaga aatagagcac tctctaatct aaaaaaagat atattaaaag
     9661 aagtaattct tattaaaaat tccaatacaa gtcctgtaga aaaaaattta cattttgtat
     9721 ggataggtgg agaagtcagt gatattgctc ttgaatacat aaaacaatgg gctgatatta
     9781 atgcagaata taatattaaa ctgtggtatg atagtgaagc attcttagtc aatacactaa
     9841 aaaaggctat agttgaatct tctaccactg aagcattaca gctactagag gaagagattc
     9901 aaaatcctca atttgataat atgaaatttt acaaaaaaag gatggaattt atatatgata
     9961 gacaaaaaag gtttataaat tattataaat ctcaaatcaa taaacctaca gtacctacaa
    10021 tagatgatat tataaagtct catctagtat ctgaatataa tagagatgaa actttattag
    10081 aatcatatag aacaaattct ttgagaaaaa taaatagtaa tcatgggata gatatcaggg
    10141 ctaatagttt gtttacagaa caagagttat taaatattta tagtcaggag ttgttaaatc
    10201 gtgggaattt agctgcagca tctgacatag taagattatt agccctaaaa aattttggcg
    10261 gagtatattt agatgttgat atgcttccag gtattcactc tgatttattt aaaacaatac
    10321 ctagacctag ctctattgga ctagaccgtt gggaaatgat aaaattagag gctattatga
    10381 agtataaaaa atatataaat aattatacat cagaaaactt tgataaactt gatcaacaat
    10441 taaaagataa ttttaaactc attatagaaa gtaaaagtga aaaatctgag atattttcta
    10501 aattagaaaa tttaaatgta tctgatcttg aaattaaaat agctttcgct ttaggcagtg
    10561 ttataaatca agccttgata tcaaaacaag gttcatatct tactaaccta gtaatagaac
    10621 aagtaaaaaa tagatatcaa tttttaaacc aacaccttaa cccagccata gagtctgaca
    10681 ataacttcac agatactact aagatttttc atgattcact atttaattca gctaccgcag
    10741 aaaactctat gtttttaaca aaaatagcac catacttaca agtaggtttt atgccagaag
    10801 ctcgctccac aataagttta agtggtccag gagcttatgc atcagcttac tatgatttca
    10861 taaatttaca agaaaatact atagaaaaaa ctttaaaagc atcagattta atagaattta
    10921 aattcccaga aaataatcta tctcaattga cagaacaaga aataaatagt ctatggagct
    10981 ttgatcaagc aagtgcaaaa tatcaatttg agaaatatgt aagagattat actggtggat
    11041 ctctttctga agacaatggg gtagacttta ataaaaatac tgccctcgac aaaaactatt
    11101 tattaaataa taaaattcca tcaaacaatg tagaagaagc tggaagtaaa aattatgttc
    11161 attatatcat acagttacaa ggagatgata taagttatga agcaacatgc aatttatttt
    11221 ctaaaaatcc taaaaatagt attattatac aacgaaatat gaatgaaagt gcaaaaagtt
    11281 actttttaag tgatgatgga gaatctattt tagaattaaa taaatatagg atacctgaaa
    11341 gattaaaaaa taaggaaaaa gtaaaagtaa cctttattgg acatggtaaa gatgaattca
    11401 acacaagcga atttgctaga ttaagtgtag attcactttc caatgagata agttcatttt
    11461 tagataccat aaaattagat atatcaccta aaaatgtaga agtaaacttg cttggatgta
    11521 atatgtttag ttatgatttt aatgttgaag aaacttatcc tggtaagtta ctattaagta
    11581 ttatggacaa aattacttcc actttacctg atgtaaataa agattctatt actataggag
    11641 caaatcaata tgaagtaaga attaatagtg agggaagaaa agaacttcta gctcactcag
    11701 gtaaatggat aaataaagag gaagctatta tgagcgattt atctagtaaa gaatacattt
    11761 tttttgattc catagataat aagctaaaag caaagtccaa gaatattcca ggtttagcgt
    11821 caatatcaga agatataaaa acattattac ttgatgcaag tgttagtcct gatacaaaat
    11881 ttattttaaa taatcttaag cttaatattg aatcttctat tggtgattac atttattatg
    11941 aaaaattaga acctgttaaa aatataatcc acaattctat agatgattta atagatgagt
    12001 tcaatctact tgaaaatgta tctgatgaat tatatgaatt aaaaaaatta aataatctag
    12061 atgagaagta tttaatatct tttgaagata tctcaaaaaa taattcaact tattctgtaa
    12121 gatttattaa caaaagtaat ggtgaatcag tttatgtaga gacagaaaaa gaaatttttt
    12181 caaaatatag cgaacatatt acaaaagaaa taagtactat aaagaatagt ataattacag
    12241 atgttaatgg taatttattg gataatatac agttagatca tacttctcaa gttaatacat
    12301 taaacgcagc attctttatt caatcattaa tagattatag tagcaataaa gatgtactga
    12361 atgatttaag tacctcagtt aaggttcaac tttatgctca actatttagt acaggtttaa
    12421 atactatata tgactctatc caattagtaa atttaatatc aaatgcagta aatgatacta
    12481 taaatgtact acctacaata acagagggga tacctattgt atctactata ttagacggaa
    12541 taaacttagg tgcagcaatt aaggaattac tagacgaaca tgacccatta ctaaaaaaag
    12601 aactagaagc taaggtgggt gttttagcaa taaatatgtc attatctata gctgcaacgg
    12661 tagcttcaat tgttggaata ggtgctgaag ttactatttt cttattacct atagctggta
    12721 tatctgcggg aataccttca ttagttaata atgaattaat attgcatgat aaggcaactt
    12781 cagtggtaaa ctattttaat catttgtctg aatctaaaga atatggccct cttaaaacag
    12841 aagatgataa aattttagtt cctattgatg atttagtaat atcagaaata gattttaata
    12901 ataattcgat aaaactagga acatgtaata tattagcaat ggagggggga tcaggacaca
    12961 cagtgactgg taatatagat cactttttct catctccata tataagctct catattcctt
    13021 cattatcagt ttattctgca ataggtataa aaacagaaaa tctagatttt tcaaaaaaaa
    13081 taatgatgtt accaaatgct ccttcaagag tgttttggtg ggaaactgga gcagttccag
    13141 gtttaagatc attggaaaat aatgggacta aattgcttga ttcaataaga gatttatacc
    13201 caggcaaatt ttactggaga ttctatgcct ttttcgatta tgcaataact acattaaaac
    13261 cagtgtatga agacactaat actaaaatta aactagataa agatactaga aactttataa
    13321 tgccaactat aactactgac gaaattagaa acaaattatc ttattcattt gatggagcag
    13381 gaggaactta ctctttatta ttatcttcat atccaatatc aatgaatata aatttatcta
    13441 aagatgattt atggatattt aatattgata atgaagtaag agaaatatct atagaaaatg
    13501 gtactattaa aaaaggaaat ttaatagaag atgttttaag taaaattgat ataaataaaa
    13561 ataaacttat tataggcaat caaacaatag atttttcagg tgatatagat aacaaagata
    13621 gatatatatt cttgacttgt gagttagatg ataaaattag tttaataata gaaataaatc
    13681 ttgttgcaaa atcttatagt ttgttattgt ctggggataa aaattatttg atatccaatt
    13741 tatctaatac tattgagaaa atcaatactt taggcctaga tagtaaaaat atagcttaca
    13801 attacactga tgaatctaat aataaatatt ttggagctat atctaaaaca agtcaaaaaa
    13861 gcataataca ttataaaaaa gacagtaaaa atatattaga attttataat ggcagtacat
    13921 tagaatttaa cagtaaagac tttattgctg aagatataaa tgtatttatg aaagatgata
    13981 ttaatactat aacaggaaaa tactatgttg ataataatac tgataaaagt atagatttct
    14041 ctatttcttt agttagtaaa aatcaagtaa aagtaaatgg attatattta aatgaatccg
    14101 tatactcatc ttaccttgat tttgtgaaaa attcagatgg acaccataat acttctaatt
    14161 ttatgaattt atttttgaac aatataagtt tctggaaatt gtttgggttt gaaaatataa
    14221 attttgtaat cgataaatac tttacccttg ttggtaaaac taatcttgga tatgtagaat
    14281 ttatttgtga caataataaa aatatagata tatattttgg tgaatggaaa acatcgtcat
    14341 ctaaaagcac tatatttagc ggaaatggta gaaatgttgt agtagagcct atatataatc
    14401 ctgatacggg tgaagatata tctacttcac tagatttttc ctatgaacct ctctatggaa
    14461 tagatagata tatcaataaa gtattgatag cacctgattt atatacaagt ttaataaata
    14521 ttaataccaa ttattattca aatgagtact accctgagat tatagttctt aacccaaata
    14581 cattccacaa aaaagtaaat ataaatttag atagttcttc ttttgagtat aaatggtcta
    14641 cagaaggaag tgactttatt ttagttagat acttagaaga aagtaataaa aaaatattac
    14701 aaaaaataag aatcaaaggt atcttatcta atactcaatc atttaataaa atgagtatag
    14761 attttaaaga tattaaaaaa ctatcattag gatatataat gagtaatttt aaatcattta
    14821 attctgaaaa tgaattagat agagatcatt taggatttaa aataatagat aataaaactt
    14881 attactatga tgaagatagt aaattagtta aaggattaat caatataaat aattcattat
    14941 tctattttga tcctatagaa tctaacttag taactggatg gcaaactatc aatggtaaaa
    15001 aatattattt tgatataaat actggagcag cttcaactag ttataaaatt attaatggta
    15061 aacactttta ttttaataat aatggtgtga tgcagttagg agtatttaaa ggacctgatg
    15121 gatttgagta ttttgcacct gccaatactc agaataataa catagaaggt caggctatag
    15181 tttatcaaag taaattctta actttgaatg gcaaaaaata ttattttgat aatgactcaa
    15241 aagcagtcac tggatggagg attattaaca atgagaaata ttactttaat cctaataatg
    15301 ctattgctgc agtcggattg caagtaattg acaataataa gtattatttc aatcctgaca
    15361 ctgctatcat ctcaaaaggt tggcagactg ttaatggtag tagatactac tttgatactg
    15421 ataccgctat tgcctttaat ggttataaaa ctattgatgg taaacacttt tattttgata
    15481 gtgattgtgt agtgaaaata ggtgtgttta gtggctctaa tggatttgaa tatttcgcac
    15541 ctgctaatac ttataataat aacatagaag gtcaggctat agtttatcaa agtaaattct
    15601 taactttgaa tggtaaaaaa tattactttg ataataactc aaaagcagtt accggatggc
    15661 aaactattga tagtaaaaaa tattacttta atactaacac tgctgaagca gctactggat
    15721 ggcaaactat tgatggtaaa aagtattact ttaatactaa cactgctgaa gcagctactg
    15781 gatggcaaac tattgatggt aaaaaatatt actttaatac taacacttct atagcttcaa
    15841 ctggttatac aattattaat ggtaaatatt tttattttaa tactgatggt attatgcaga
    15901 taggagtgtt taaagtacct aatggatttg aatactttgc ac
//
LOCUS       DAEPOG010000087        15879 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEPOG010000087 DAEPOG010000000
VERSION     DAEPOG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.087"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            138..449
                     /locus_tag="KR432_002233"
     CDS             138..449
                     /locus_tag="KR432_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0640764.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            471..1784
                     /locus_tag="KR432_002234"
     CDS             471..1784
                     /locus_tag="KR432_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0640765.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            1861..3171
                     /locus_tag="KR432_002235"
     CDS             1861..3171
                     /locus_tag="KR432_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0640766.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            3197..3928
                     /gene="chbG"
                     /locus_tag="KR432_002236"
     CDS             3197..3928
                     /gene="chbG"
                     /locus_tag="KR432_002236"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH0640767.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            3941..4258
                     /locus_tag="KR432_002237"
     CDS             3941..4258
                     /locus_tag="KR432_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0640768.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(4370..4834)
                     /locus_tag="KR432_002238"
     CDS             complement(4370..4834)
                     /locus_tag="KR432_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640769.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            5104..6042
                     /locus_tag="KR432_002239"
     CDS             5104..6042
                     /locus_tag="KR432_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640770.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            6170..7183
                     /locus_tag="KR432_002240"
     CDS             6170..7183
                     /locus_tag="KR432_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0640771.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            7189..8211
                     /locus_tag="KR432_002241"
     CDS             7189..8211
                     /locus_tag="KR432_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0640772.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            8226..9008
                     /locus_tag="KR432_002242"
     CDS             8226..9008
                     /locus_tag="KR432_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640773.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            9020..10360
                     /locus_tag="KR432_002243"
     CDS             9020..10360
                     /locus_tag="KR432_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0640774.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(10455..12735)
                     /locus_tag="KR432_002244"
                     /pseudo
     CDS             complement(10455..12735)
                     /locus_tag="KR432_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(13072..14235)
                     /locus_tag="KR432_002245"
     CDS             complement(13072..14235)
                     /locus_tag="KR432_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0640775.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(14248..14523)
                     /locus_tag="KR432_002246"
     CDS             complement(14248..14523)
                     /locus_tag="KR432_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0640776.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(14544..15518)
                     /locus_tag="KR432_002247"
     CDS             complement(14544..15518)
                     /locus_tag="KR432_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0640777.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            complement(15789..>15879)
                     /locus_tag="KR432_002248"
     CDS             complement(15789..>15879)
                     /locus_tag="KR432_002248"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640778.1"
                     /translation="NRNTTKTCEILKITRSTLWRKLKEIEDDV"
BASE COUNT         5601 a         2095 c         2682 g         5501 t
ORIGIN      
        1 agaattaaat gtatatacat ttaaaaataa gatatataca ttaagaaaaa attaatagaa
       61 aagcatattt taaatgtata gtttaaaaaa tttatgactt aaaaaatatt taatgaatct
      121 aatttggagg ggaaaaaatg ataaaaataa tgttagcttg ttcagcagga atgtcaacta
      181 gtttattagt tacaaaaatg gaggatgcag caaaggaaaa cggtatagaa gcaaaaatat
      241 gggctatatc agaagtgaac ttaaaaaatg aaatagaaaa ttgtgatgtc ttacttttag
      301 ggcctcaagt tagatatgta ttaggaaaag caactgaaat ggcaaaacca tataatattc
      361 cagtagaagt tataaatatg atggattatg gaagatgtaa tggaaaagct gtgttagata
      421 gagctgtaga gttaaatagt tcaaaataaa gttgtactgg aggatgtaaa atggaaaaat
      481 tcatgtcatt tatggataaa tatatagtgc ctgtagctgc taaaatagga gctcaaaggc
      541 acttagtagc agtaagagat gcatttatag taatgatacc tataactatg gtaggtgcct
      601 tgggaacatt gataaataac ttaccattgg aagcatacaa aaatttgatg gcaagtatat
      661 ttggagaaaa ttggaccaca tttggaggcg acctttggtg gggagcaata ggtacaatgg
      721 cagtattctt agtaataggt gttgcttatt ttctagcaaa atcttatgag agtgatgggt
      781 tacaatctgg gcttatagct ttatctatct tttttataat ggctccacaa attggtaaaa
      841 tagttcctga gggaggaact acagtagttg aaggatgggg aatgatacaa caaacctact
      901 taggtacagc agctttattc tcttctatat taataggatt attatcaact gagatatttg
      961 taagattatc taaggtaaag aaattaacta taaaaatgcc agatggagtt ccaccagcag
     1021 tttcaagatc atttgcaaag ttaataccag gaatgttaac tataatgata tttactgtaa
     1081 taggaatatt tataaaaatg ctttctaatg gaagtttctt aacagatatt ttaaatactt
     1141 atttaggagc accgctatca aatgtagctg atagtctagg ttctacaatg cttattgcat
     1201 ttataattca tatactttgg actgttggac ttcatggtgc aaatatagca cttcctttta
     1261 cagaaactat acttatgaaa ttaggtggag aaaatgctgc cttggctcaa gcaggagcaa
     1321 cggaaggata ccatgtactt gcaggtgcat tctttgatgc atttgtatat ttaggtggtt
     1381 ctggaatggt attgggatta atagtagctt tattaatagc tgggagaaga cgtaaagaaa
     1441 tgatagtatt aggtgggcca cctgcaatat ttaatatagg agaaccattg atattcggac
     1501 ttccaatagt tttaaatcct atatttatga ttccatttgt acttgcacca gtgatatgta
     1561 gtgctgtatc atacttagca atagattttg gattagtagc accagtaatt ttacctaaaa
     1621 taccttgggt aacacctcct atattaggtg gagcgatggc tacaggtgac tggactggtg
     1681 gagctttagc actatttaat ttaatattat caatattaat atatattcca tttgtcatag
     1741 cgtctgaaaa aatggaagct aataaattga aaattaacaa ctaggtagtt ttatttcggt
     1801 aaataaatgt atagggtttt gtagagtata ttaaattata ggtaaggatg gtataatgag
     1861 atgaaaaagg gactaaaaat agttacaatt ggaggaggtt caagttatac tcctgagctt
     1921 gtagagggtt ttataaatag atatgaagag ttacctgtaa aggaattatg gcttgtagat
     1981 atagaagctg gaaaagaaaa attagagata gttggaaatc tagctaagag gatggtaaaa
     2041 aaagctggag tagatatgaa aataaatctt actttagata gaagagaagc tctaagagat
     2101 gcagactttg tgacaacaca acttagagta ggtcttttag atgcgagaat aaaagatgaa
     2161 actattcctt tatctcatgg agttatgggg caggaaacta atggtgctgg aggtttattt
     2221 aaagcccttc gcacaatacc agttatattt gatataataa aagatgtgga agaattatgt
     2281 ccaaatgcat ggatggtgaa ttttacaaat ccaacaggaa taataacaga ggctgtattc
     2341 aaatatacta attttagaag atatataggt ctatgcaatg tgccaattca tttaaaaaat
     2401 gatgttgcca aactatttaa tgtagaaagt gacagaataa gtatggattt tgcaggtctt
     2461 aatcatatgg tttatggtct aaatgtatct ttagatggag aagatgttac aaaagaagca
     2521 attgataagt ttgtcaaagc agatataagt atgcaaaata taaaggcaat tgattttaat
     2581 gctgaatttg taaaatcttt gggtgcaata ccttgtccat atcatagata ctactataaa
     2641 actaaagaaa tgcttgagga tgagttgaga gagtttaaag agggaaaagc tagaggtcaa
     2701 gtagttaaag aattggaaga acaattattt gaattatata aggatgaaaa attagatgta
     2761 aaaccacctc aacttgaaaa aagaggaggg gcttactata gtgatgctgc ttgtaattta
     2821 ataagttcta tatacaatga caaaaaagat atccaagtag ttaatacatt aaacaatggt
     2881 tctataagag attttaaaga tgaacaagcg gtagaagtga gtagtgtaat aaccaaaaat
     2941 ggtccaaaac caataagtat aggatattta ccagattctg tgcatggatt agtaagccaa
     3001 atcaaatcat ttgaagtatt ggtagctaaa gcagctgtat atggcgatta tgaatctgct
     3061 cttttggcac tttgtataaa cccattgatt ccatctgatg acttagcaaa aactatatta
     3121 gacgaaatgt tggaagctca taaagattat cttcctagat ttaatagata gttgtctata
     3181 gaaagtaggt tatggtatga ctaaaattat aataaatgca gatgattttg gatattgtga
     3241 agctgtgaac tatggaataa tttcagctca taataatggt atagttaggt ctacttctat
     3301 gatggcaaat atgcctgggg tagaacatgg agtaggctta cttaaagaaa ataaaacttt
     3361 aaattgtgga gttcatatga ctttaagttg tggtagacca ttattatcta acttaaaaac
     3421 tattgttgat aaagacggtt tttttattag gaggattaca gacgaaatta ttgaaaaaat
     3481 ggattgtgat gagatttata gagaactttg tgctcaaata gatagggtaa aagggcttgg
     3541 aatagatata tctcatttag atagtcacca tcatatccat actttagtaa gtttaaagcc
     3601 agtagtagag aagattgtaa ccaagtataa tcttccaata agaggtggat ttgaatataa
     3661 tttagagtat tctaaagtag ttcctttaat agatagtttt tataaggaaa atgtaagtga
     3721 agaatatttt ataaaaaata tagacgaaat aatgaagtat gatgtagtgg atattatgtc
     3781 tcacccagct tttttagatg attatatttt aaattctaca tcttatgcta ttgatagaac
     3841 gaaagagcac aagatactta catctaaaaa agtcaaagaa tttttagaaa agaatggatt
     3901 agtcatatca agctatagag atatttagga ggtcgaataa atggatgaga agatgataga
     3961 gatatctttt ggaataattg gatttgcagg tgatgcgaaa gggctagcat ttgaagcaat
     4021 aaaagaagca aagagcggga atatagataa agctagagaa tgtattgaaa agtcgaaaga
     4081 atcaattcat aaagctcata aattccaaac agaattgata caaaatgaag ctaatggtaa
     4141 taaaactgaa ataagtgtta tacttattca tgcacaagac catttaatga cagctatgaa
     4201 ttttcaacaa ttagcagaag aatttataga tgtatatgaa agattagaaa gtaagtaatt
     4261 taaatttatt attaaaaaag agtgtataaa aataattttt ataattatta aatgcactct
     4321 ttttttattt aagtagagtt tagcaatata gtattatgtt attgatactt taaaatttag
     4381 acttctcata attgtaaggt atactagtac caacaactac atcttcaatt ttttcaacaa
     4441 taagccaatc atccatattt ccttgatggt caaaatcaag aggtgttatt tcttcaacat
     4501 tttcaaattc aactaagcat aaacattttt tatgccaacg aactttttgc ttttcagata
     4561 aatttaattt tggttgattt tcatttaaaa ttttagaaat ttcatcttct gttaatttaa
     4621 catagttttc tactttttta acagtggctt ttgcagatat ttttccagtt cctttttcca
     4681 taaaataaag taactcatct tcaaatactc tactatgtgg tatttttcta ccagcagcac
     4741 ctctaataac cattgttttt gttccagcca agattttatc taagaccttt tctccttttt
     4801 ttcctgcatt atcacaatat actaaatgta ccatgttttg aatcctcctt actaaattca
     4861 ttatctataa attgcatttt atcattgttt aacctaaaag tcattgtgtt tgtgtccaat
     4921 tcttaaaaaa aatactgttt aatataagaa aaccttatct aagctatata taattaagat
     4981 taattaggaa tttttatcct tttacaaaat aatattgact aaaaatagaa aaatgctata
     5041 ataaatatgt gcttatgata atcaatatca tgtaagtata ataaacaagt aggagaagta
     5101 atcatgaaaa aaataaaatc acttgcaata tttatatcta ttataactac gttagtactt
     5161 gttacagcat gttcagataa gaatacagag gataaagata aatcagagac aagagttgta
     5221 caatctgtta aaggagaggt taagatacca tcaaatccta aaaaaatagt tgatatatca
     5281 ggaagtagtg aagaactatt attagcaggt tataagcctg ttgcaacagc aaatgtagac
     5341 tcatatgaaa ctgataaatt accaagttac ataagagaag agcttaaagg tgtaaagata
     5401 gttgggcact caatgatgga tactatggat atggaagcta tattagaagt aaatcctgat
     5461 ttaataataa tgagtcaaag acaagaaaaa atatatgacc aattaaaaga aatagctcct
     5521 gtagttatga tgaaagatta tgcaaatgac tggagaagca agttaactga tgtttcaaag
     5581 ttatttgaca aagaagaaga agctaaaagt tggctacaga agtatgatga aaaagctaca
     5641 aagttaggta aggaagttat agaaaagaat ggagaaaaaa cttatttacc agtgttggca
     5701 agttcaggtc aatttatggt atttagtgat ggtggaatag gtactctaat caatgatgat
     5761 atgaaattag ctagacctaa gaatatgcca aaacaggatg gaataacact accaatggta
     5821 agtatggaag gtttaacaga tatagatgca gaccatatag ttgtaattgc tacagaagca
     5881 gataaaaaag atttagaaaa tagtgctatc tggtctcaaa taagagcagt aaaagatggt
     5941 aatgtaacaa tacttgatgc tgctccattt ttcagtcaat catacaatcc aattggaaaa
     6001 gagttattat tagaatcagt taaaaatgaa ttaacaaagt aattcttaac ttagtaggtg
     6061 tgagataatg atattcattt ttcacaccta tttttaataa atcttaaaat taaaatactt
     6121 aatagatgat attatactga cgattaaaag ggaaaaggag gtaggataat tgactaagct
     6181 gagtaaaaaa aatataactt atttgttgat attagtaagt atagttgtac ttgtatttgg
     6241 aattgtatta tccataacta ttggggcaaa agatatgaat ctatcaactg tcatagatag
     6301 tttgataaaa atggaagatg gaataaatat gagaatagta aaggatgtta gacttccaag
     6361 agcaatagca gctgcacttg ttggagggtt tttagcagtt tctggggcaa taatgcaagg
     6421 aataacgaga aatcctatag ctgaaccatc tgttatagga attacacaag gagctacatt
     6481 cgcaatttcg atatcactgg tacttcaaaa aaaacttcct caacttatac atggaagttt
     6541 tagtgtaatg atgtttgcat ttattggagc aagtataagt ggattattta tatattttat
     6601 aagttctaaa tcaagaggaa gagttaataa tgtaaaatta gctcttgcag gagtcgctct
     6661 tggtacattg ctaatttctt tggcatcagc aatatctatg tattttaacc tatctcaaca
     6721 attaagtttc tggatatcag gagggcttgt aggagtgaag tgggaaggta taaaattgct
     6781 atttgtagct ggtggtattg gatttatttt agctattatt atggctccaa gaataactat
     6841 attaagtctt ggagaagaag tggcaatagg attaggacaa aaaactaact ttgttagatt
     6901 tatatgtata gtacttgtaa tattaatgac tggagcatct gtgtcagtgt ctggtaatat
     6961 tatttttata ggtcttatag ttcctcaaat agctaaagga atagtaggtt cagattataa
     7021 gtatataata ccgagctctt tagtattagg agctgtattg cttgtatata cagatatatt
     7081 aagtaggatg ataaatcctc catatgagac accagtaggt tctataacag cacttatagg
     7141 agtgccaata tttatatacc ttgttagaaa gggggagaaa taggtactat gaaaaatatt
     7201 ataaaaataa attctaaaac attacttgtt atatcaattt taatggtgct tatttttatc
     7261 acttttttat tgagtttaaa tatgggctca ttttctatag aaccaatgga tgttataaag
     7321 acttttttag ggcagggtca gcgtaatcat gaaattgcaa tatttaaatt aagattacca
     7381 agaatagtaa tagcactttt ggttggttct gcattatcaa cagcaggtgt aatacttcaa
     7441 ggagttacaa agaatgattt agctgattct ggaattttgg gaataaattc aggtgctgca
     7501 ttgtttgtag tagtttatat ataccttatg aatggtaatg tatatgaagg tatgagtaac
     7561 ttgactgtat ttacaatgcc tattgttgca cttataggag ctttatttgg agcatttctt
     7621 atatattctc ttgcatggaa aggtggtata aattcgtcaa gattactact tattggtata
     7681 ggtgtaaaca tagcttttac ttcaattttg acgatattcc aattgaaatt tacaacacaa
     7741 gaatttaata gggttatggc atggacatca ggaagcattt ggggaaccag ttggaaatat
     7801 gtaatagctg tgcttccatt tatagtgatt tttatgggta taactattta taaatctaaa
     7861 tatatagatg ttttaaattt gggtgatgaa gtatctataa gtcttggtgt agatgtagaa
     7921 aaacaaagaa gaagacttat aatatatgca gtaattttat ctggtgtatc gacagcagta
     7981 gcaggtagta tttcattttt aggtttaata gctccacata taggtagaaa aataatagga
     8041 ccaaagcata aaagattaat accagtatca gcattgatag gaacattatt acttttaata
     8101 gcagatacta tatcaagaaa tcttctagca cctatagaaa taccagtagg tatagtagtt
     8161 tctataatag gagtgccgta ctttatttat ttaatgttgg taaatgatta ggaaagggtg
     8221 ttgttatgaa ctgtataaat actaagaatc taaatgtatc atatggaaac acagatatta
     8281 taaaaaattt aaatttaaat ataccaaaag gaaaaataac taccatcata ggggctaatg
     8341 gttgtggaaa atcgacaata cttaaaacta taggtagaat tattagccct aatagtggga
     8401 aaatatatat aaatggagag gacatctaca aaaagaagtc taaagaaata gcaaaggata
     8461 tggctgtact tccacaaagc cctcaagctc ctagtggtct taccgtttca gaattaattt
     8521 cttatggaag atttcctcat aaaagtggat ttggtaattc tagtaaagaa gacagagaga
     8581 ttgtaaattg gagtttagaa gttactggaa ttgcagaatt taaggataga aatatggaag
     8641 atttatcagg aggacaaaga caaagggcat ggatagctat ggctttggca caagaaactg
     8701 atatattatt actggatgag cctacaacat atttagattt ggcacatcaa ttagagatac
     8761 taaaattatt agatgaacta aataaaaaac aaggaagaac tattgtcatg gtaatacatg
     8821 aacttaataa tgcagcaaga tttgctgacc acatgatagg tattaagaaa ggtaaaatag
     8881 tttgtgaagg tgatgcatat gatgttatga cgaaggataa tctaaaagaa ttatttaata
     8941 ttgatgcaga aatagcaaca gacccaagaa caaataaacc aatttgtata acttatgaca
     9001 tggtgtaaat ggtgacaaca tggagaatca agaagcatta agacatgaaa aaatatggat
     9061 tttattattt aggtattcga taccggcaat aatagcaatg atggtaactt cactttataa
     9121 tgtagtagat agagctttta ttggttctat ggaaggtatt ggctctatag ctattgcagg
     9181 tcttggtgta actatgccag tatttactct aataatagct tttggaatgt tggtatcagt
     9241 aggtgctagc acaagattgt ctataaaatt aggggaaaga aatagagaag aagcagagaa
     9301 gatattaggt aatgctttga cattatctat aattatctct ttaattataa ctatattagg
     9361 attagttttc ttagaagata tactttttat tttgggtgca agtaaagatt caatatttta
     9421 tgcaaaagac tatatgagtg tcatattgat tggaagtata tttaatttag ttgcattttc
     9481 tcttaataat gcaattagag cagaaggtaa tccaaaatta gcagcaagga caatgattgt
     9541 aggatgtgta ttaaacctga tattggaccc tatatttata tttgtgttta atttaggtat
     9601 aaaaggagca gctatagcaa cagtactatg tcaaatagta gtattctttt gggtaacata
     9661 ttattttata agagggaagt ccactttaaa actaaaaaaa tgtaacttaa aattgaataa
     9721 agatatagta aagaaggtat ttgcaatagg aatgacacct tttgctatgg aagtagctat
     9781 aagtataact cacatgttta caaacaattc tctaaaggtt tatggtggag atttagctat
     9841 tggggctatg acagcactta catctatttt attgatgttt atgatgcctg tgtttggatt
     9901 gaatcaaggt atgcaaacta taatatcata caattatggt gcaaaacaat atgaacgtgc
     9961 aaagaaaact ttaatattat ctatcatagt gtcagttgtc atattgtcat ttggattttt
    10021 aatagtacaa gttttcccag aagtatttgt aggtatattt aataaagatt cagatttgat
    10081 ggatatagca gtaagaggta taagcataaa tctaattaca ttgcctataa tggggatttc
    10141 tattgtagga cctgtgtatt ttcagtgtat aagtaaagtt aaacattcaa tgtttttaac
    10201 tcttttaaga caatttattt tatttatacc attaatcatt gtacttccaa ttcaatttaa
    10261 tttagatggt gtttggttgg cacagccagt tgcagatttt attgctatga taatcgtatt
    10321 attattctta aaaagggaat ttaaaacaag ccgagtataa gaaatttaga aaataaaatt
    10381 aaattggaaa ataaaaagac ttgtctttat aaaatgatta aaattatttt atagagacaa
    10441 gttttttatt ttatttaaac ggaactatcc atactggttt cttctagttt ttttccaaaa
    10501 gtcattttct caaatatttc ttgaggcaga ggttttgaaa aaacatatcc ttgtatcata
    10561 tcacaattaa tttttttaag ataatccatt tgcaagattg tttcaacacc ttctgatata
    10621 ctattcatat ttaatttttt tgccaaatta actatagatt ctattacatg attttcagca
    10681 atattatctg cattaggtga gctaaaaaaa gcacggtcaa gttttatatg tgtcaacttt
    10741 tatttctttt aacatattga gtgaagagta tccacttcca aagtcatcta gtgaacattg
    10801 aaaaccttct tcatggattt ggtctattat attaattaat ttttggagat tttcaaatac
    10861 aagtgtttca gttaattcaa tttctaagag cttagcagga atgttgtatt tattacgaag
    10921 cattctgtat ttttctaaaa aatctatatt atttaaatta gcacgtgaaa aattaactga
    10981 aacaggcatt ggagtttctc cattatctat ccattttctt atcatactac atactttttc
    11041 aaaaacatag tggtcgagtt ttgtaataaa accatttttt tcaaagaaag gaataaactc
    11101 gtctggtggt ataaggcctt tatttgggtc ttgccatctt actaatgctt ctgcacctac
    11161 aatagtatta tctttgaggc ttattttagg ctgaagatat actataaatt cgttattatt
    11221 taatgcttcc tccattctat tttccatttc tttttcttta atcattttta aacgctctaa
    11281 atcactataa aaaacacatg tataaagtgg atgatttcca atgtttcctt tattattttt
    11341 tcgtgcaaca tttgcacggt cttgaattga aactaaaggt agatttgggt catcaatttt
    11401 atatacacct atagatagag ctataaaata tttacattct tcatttttat tgaaactatt
    11461 aatatcattg gtgatatttt ctatgtactt aagaatttct ttttgaggaa tagttttaat
    11521 aagtacatta aactggtcat tagaaactcg acaaagcaat tctcctggtt ctagataatt
    11581 actaatgaca tcatgtatgt actttaatgt tttatctcct gcttcactac caaaagaatc
    11641 attaataagt ttgaatttct gaatatttat agataccaat gaatagcttt ctgatgaaga
    11701 tgactgtata atttttttag cttcaatatc aaaacgtgct ttactgtaac ctttagttac
    11761 agagtctaca taggcgattt ttataagttg tgccctgttc ttttgattta taaacataat
    11821 caatataata agcaataaaa atagtataac aattaaaata ttgatgaaga ttgctttttt
    11881 tagaagtaaa tcaaactgtg cacttgctgt ttttgttgga acaactgaaa ggaggtataa
    11941 gttttcttct tgtaatggaa gataatgcat tgccttagaa ataccatcat ttaatgtata
    12001 atacatagaa ccagttttgt tcttttttaa gtttattttc atttcttcaa gtttattttt
    12061 atcaacctgt tttccttggt gtttaatcat gtcaaaaaaa tttaccacat tttttggagc
    12121 atttttgttt gctgaactta caataaaatt tccatcttgg tcgataattt gggaaaatcc
    12181 ttcaccatga aagctttcta cacttaataa atttctcata gtatcaatag tattccaagc
    12241 agcaacagct ccaacaattt tatcgccatt gtatagtggt acagaatata aaattccata
    12301 tgtaccagat tgtgatttaa caggagtaat tgtttcttta ccttgaaggg cttttctata
    12361 aaagtcttgt cctttaacat tacctgtatc tttaagggca cttacaaaat ttccattaga
    12421 atctataata cctaaccaat caaattcaaa taattttaat ttttttgata agtatctatt
    12481 gacttctttt tcactatcat taatctcaaa ataagtatct cccatagaat atagtgtcct
    12541 aaatatactt ttacatttca cattaattgt agtggaaata ctttgtgaaa cttctacaag
    12601 atatcgttct gtttcattat gtgttacttt atttagattc ataataaaat tgacacatgt
    12661 taatgacacg attaaaagga gtgtcaatag taaagtagct attatttttt tattttttgt
    12721 gttttcaaag ttcattcttt tgtttcatcc tccatccttt atttgtttac aaataagaat
    12781 tctctcagac atattatacc aaatatcttt tcatacaaag atattaaatt ttatttatct
    12841 cactttacat ttagctttat ttatgtcttc ttagagatta atcaagacac gtttattgtt
    12901 taaatcttat tttataatat tttgagtaaa agaggttaaa aaaggaactg ttttcaaaat
    12961 aattttgata cagttccttt tcagtataga aaatttttat aggattagaa agaactttta
    13021 gcttatgtat ctacataagt attatattta taaaagatat tttgttacaa tctaaaatgt
    13081 agatgaaatt ctgtaaatgc caaattctct atgaccaaga ctttcagctt tggtatgttt
    13141 accattacag acttctatta tttcattaaa tatttcttga cctatatctg ctattttagc
    13201 accatctgtt ataattctac ctgcatttat atcgatattg tctggcattt tgttgtatgt
    13261 atcactattc ccagtgattt taataactgg tgaaataggg cttcctgttg gagtacctct
    13321 accagttgaa aagattacaa tttgtgcacc acctgctacc ataccagtga tagaatctat
    13381 atcttgacct ggagtatcca taaaatataa tcctttttta tcaggaggta caatatcagc
    13441 atattcaaga gcacctttga atggcttatt gcctgcctta tacatacacc caagtgattt
    13501 ttcttcaatt gttgaaagtc caccagcttt attacctgga gtaggttgac cactacgtaa
    13561 atcttctccc atagcaattg ctcttttttc tacatcactt acaaatttaa gaaacttatc
    13621 tttcatttct tcatcttcaa aacgggtagc caacaaatgt tctgcaccaa ttacttctgt
    13681 agtctcactt agtatgctac ttccaccttc atctacaaga aggtttgatg caacgcctat
    13741 tgaagggttt gaagctattc cacttgttgg gtcagaacca ccacattcaa gtgctaatgt
    13801 aatttcactc ataccaaatt caactcttac ttcttttgat acttcacgag ccattttact
    13861 tactatttca gttcccttag aaactgtttt taatgttcca ccacattctt gtataacaac
    13921 gtaatctacc ttttttcctg ttgtagctat ttcagatgct aattcttttg cttgaatacc
    13981 atcacatcca agaccaacta ctaaaactgc ggcaacatta ggattttttc caaatcctat
    14041 taatgtcttt gctgtaagct ctatatcaga accaatttga caacatccat gttgatgagg
    14101 gattgctatt gctccaggca caagtgatgc tattcttgta gctgtctcac ttgaacatac
    14161 agatgttgga ataattaaaa catgattacg aataccaaac tgaccatttt ctcttaaata
    14221 acccattaat ttcattttaa cttcctccta ttttttatct ccacgaccac gtgtactttc
    14281 aatgttatgt acatgaacat agtcaccagt ttttatgtca actgttgcaa ttcctataat
    14341 ttcaccgtat ttgtatacaa catcaccttt ttttacatct tctgtagcca tcttatgaaa
    14401 gacaggtata ttatcattta ctatgtaaga ctttccatca attgaaactt tttcaccttt
    14461 aactaaaggt ctgacacatg taactagatt atctttttca tttgcatgaa taattgcatt
    14521 cattaatttt acctccttaa gtattaattt acttcaatat ttttatttgt tttaaaaata
    14581 gattttttct ttttttctct tttatctaga taggaaacta acattggaca acatattgct
    14641 gtaacaataa tagcagcagc aacctgtact gttgaaacag ctgctattgt agctagagta
    14701 gggtctatag ctgctaaagc ttcaggagta ccagctgcat ttccggctgt tgtacctgta
    14761 gctgcaccta ctgctggatg ttctgcacgt ataagtttca ttacaaagta gccacccata
    14821 cctgttatta tagtaactcc taaaccaagt acaactccag gtagtccagc agttataatt
    14881 tgattaaagt ttaatgctgc tcctaaagga aaagcgaaga aaggaattaa taatgttgta
    14941 ccaggtgcaa gaaattctcg tatatcttca tcaaggttac caagtataaa tccaagtagt
    15001 attggtataa gtgctgcaaa tagtgctact ataggtacat ttgcaagtcc agttactcca
    15061 aaagcaagca ttgtaaaaaa aggaccatca tttagagata agatagatat tgctccaaca
    15121 tctgtggagt caccatattc acctgcaaga gcagcaaata gacctccatt tgaattagtc
    15181 atggtagcaa ctatggcaag aggtgtaata ccaattatac ctttaggtcc aataaattta
    15241 ctaacaataa tacctaatac tgcaccaatt ataaattttg ttgctgtaag tactatacct
    15301 ttagtcaatg gcttaccagc ttgcctgata ttgatttgag ctccattgca tagacaaaat
    15361 agagcaagta tactcattgc accagtttta aataaggatg ttgtgaatcc tccaatttca
    15421 agagcttgtg gacatactgt attaaatagt gctcccaata acaagggaca gaccataagt
    15481 cctcctggta tctttttcac tgattttaag atgtgcatct agtatcactc cctttcattt
    15541 ttatataggt attataaaag caattactat gccaatatat agagtttgta tttttagtta
    15601 tcatgattat ttttaataat cgattgaaat gctgtagatt acttataatt aaaggtatga
    15661 ttgatgagaa tttcacttac aaagattctt tttatcatat aattgtattt tttattgaaa
    15721 acaaataaac gccttatgta ttaaaattaa acataaaacg ttttgatata tataggtatg
    15781 tattattttt aaacatcatc ttctatttct tttaacttac gccatagagt ggagcgagtt
    15841 atctttagaa tttcacaggt ttttgtagta tttctatta
//
LOCUS       DAEPOG010000088        15852 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEPOG010000088 DAEPOG010000000
VERSION     DAEPOG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.088"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            142..1332
                     /locus_tag="KR432_002249"
     CDS             142..1332
                     /locus_tag="KR432_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0640779.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1502..2464
                     /locus_tag="KR432_002250"
     CDS             1502..2464
                     /locus_tag="KR432_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0640780.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2582..3244)
                     /gene="zmp1"
                     /locus_tag="KR432_002251"
     CDS             complement(2582..3244)
                     /gene="zmp1"
                     /locus_tag="KR432_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBH0640781.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3344..3429)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3615..6569)
                     /locus_tag="KR432_002252"
     CDS             complement(3615..6569)
                     /locus_tag="KR432_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0640782.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      complement(6682..6767)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6893..8071)
                     /locus_tag="KR432_002253"
     CDS             complement(6893..8071)
                     /locus_tag="KR432_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0640783.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8443..8628
                     /locus_tag="KR432_002254"
     CDS             8443..8628
                     /locus_tag="KR432_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0640784.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8693..11467)
                     /locus_tag="KR432_002255"
     CDS             complement(8693..11467)
                     /locus_tag="KR432_002255"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0640785.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(11639..12895)
                     /locus_tag="KR432_002256"
     CDS             complement(11639..12895)
                     /locus_tag="KR432_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0640786.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12967..14469)
                     /locus_tag="KR432_002257"
     CDS             complement(12967..14469)
                     /locus_tag="KR432_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0640787.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14560..15750)
                     /locus_tag="KR432_002258"
     CDS             complement(14560..15750)
                     /locus_tag="KR432_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640788.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4974 a         2744 c         1925 g         6209 t
ORIGIN      
        1 ttaatatgca ctgtacataa atatatttta tataaaagtt tatattttca aacaagtaat
       61 ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata tatacataag caatataaat
      121 ctataaaaag gagtgttaca tatgatatct aataaaatgc aaactttagt tgcaaatagt
      181 tctgttataa gagctatgtt tgaagaagga aagaaacttt ctgatattta tggtgaggaa
      241 aatgtttttg attttagtat aggaaatcca agtgttgaac ctccagagac tattaaagca
      301 gttataaatg atattttaaa tgaagaatct ccaaatttag ttcatggata tatgaacaac
      361 tcaggatatg aggatgtaag agatgctatt gctgaacata taaataaaaa agatggatta
      421 aatcttacaa gagaaaattt aattatgact tgtggtgctg ctggtggtct aaatattatt
      481 ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt ttgcaccgta ttttggtgag
      541 tataaaaatt atacagaaaa ctatgatgga aaattaatag aagttcctac aaatatagag
      601 acttttgaac cagatttaga tgctctaaaa aatgctatta ctccaaaaac aagagcttta
      661 ataataaaca ctccaaataa tcctactggt gtcatatatt ctgaagaatt attaaaaaat
      721 cttggagaat tattggattc aaaacaaaaa gaatttaata caagcatata tctaatttct
      781 gatgagccat atagagaaat aatttatgat ggtgccaaag ttccttgtgt actaaaatac
      841 tatagaaatt catttatagg gtactcttat agtaaatcat tatctctacc tggtgaacgt
      901 ataggatata ttgttgctaa tgggcaaatg gatgattttg atgatgttat gtcttcatta
      961 aatgttgcaa atagaatact tggatttgta aatgctcctt ctctttttca aagggttatt
     1021 gcaagaagtt tagatgcaga agttgatgta aatatttata aaaagaattt tgaccttcta
     1081 tataatagtt taatcgatat gggatattct tgtgttaaac ctaatggtac attctatttg
     1141 ttcccaaaag ctccaattga agatgataaa aaattctgca atgatgcaaa acaatttaat
     1201 ttattattag taccaggttc ttcttttggt tgtccaggtc attttagagt atcatattgt
     1261 gtatcttatg ataaagttaa aagttcttta ccagcatttg aaaaattagc caaattatat
     1321 aatttaaaat aactaaatca aagtaattca aataattaac taccttagta tagaatactt
     1381 acattctata ttaaggtagt ttataatttt atgtactata ttttatacat accatattat
     1441 tccaatatgc ttgaatattc ttatctatag tactaatata aattacaagg aggtgtttga
     1501 cttgaaaaaa ggttatgttt atataatact gactacctta ttttttagtt caatggaaat
     1561 tagtctaaaa actgtcacta atgactttaa tcccttacaa ataactttat caagattttt
     1621 tgttggcggt ttagtcttac taccttttgc tgtaaaaaga ttaagagctc ttagtctttc
     1681 tattacaaaa tcagacttaa aatactttgc atttttaggt tttatgtgtg tagtagtaag
     1741 tatgtcattg tatcaactgg ctgttctaaa cacaaaggca tctgtagttg ctgttttgtt
     1801 cagttgtaat ccagtctttg taatgctttt agcttactta attttgaagg aaaaaatata
     1861 taggcacaat gtaatttcac ttatattaga agttcttgga attatagtta taataaatcc
     1921 attgcataca aaactaactc ttagtggaat attcttaaca ttatcttctg ctattatttt
     1981 ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa tttggtggaa tagttgtaac
     2041 ttgctttagc tttatatttg gtagtttgga aatgttgata ttggttttac tcactaaaat
     2101 taattttatc gcagatatat taaataaaaa taaacttcat atatttgcaa atatccctat
     2161 ttttagtgga tataccttac acaatatgcc cattatgatt tatgtattcg tatttgttac
     2221 aggtgttggt tatgcacttt actttatggc aatggaagct acatctacta gtcttacatc
     2281 tttagtattc ttctttaaac cagtactatc tccaatacta gcattattaa tacttaaaga
     2341 aattatccct ataaacatgg ttataggaat tttattgata gtaattggtt caataatttc
     2401 tattatacct acgataatta ctcaaatgca taataaacac gttgaagcct tgtataagga
     2461 gtagtaattg ataatctatc aagttataca taagacaaaa tttattagat ttaaacgcaa
     2521 tataaaagag atgctattat aatagcatct cttttatatt tattttaaat ttattaaagc
     2581 tctatttagc taggttttgc aaaaagctat aagtttgtgg acaagctgat tttaacttac
     2641 tatttgtatc ttgatttaaa tagtaatatg caaatgcttc tgcaaaaaat tcttctggat
     2701 atacacctaa gtagtttaca ttacctaaag aacgaccttc tttagcaaat atttgtttga
     2761 attgtgctga tttagatata tcatttaata ctatatggtc tattgcatgt gctgtttcat
     2821 gtaattctaa atttattgca tcatgccctt tacctttgtt actaaatcct attctaagag
     2881 ctactgtact tccacctaag cctggaacat catcccaagt ttttccagta ccttcccatc
     2941 ccttaggaac tacacctttt aaataagcat attctttttc atctgttaac tttccactta
     3001 ataattttat ttttaagtta tcttgcttta atacatttaa atactttcca tctatattag
     3061 ctaatctatt taccatagca ttagcttcgt ttttattgta atttccagtt ggaaaaacta
     3121 ctatttgact aagtgtgtct ttattttgtt gtatagtagt actatatgca tgtgcactta
     3181 ctggaactga acttattaag aatattgata ttatagctat taataatttt ttacttggtc
     3241 tcatattatt ccctcctttg agttaggccg ggagtaataa atttcaaaat aaaaaagata
     3301 cccaagagta tctttgtata tacaaaaaga aatttttact cttttcggca actggctggc
     3361 atggtttact aaactttagc ccctatagct ttgcgtgact aggtttccct agttttgcct
     3421 catttaattt tatatctata ttatagctta tatcttttta attgtctata tccctttgga
     3481 acttttttct aaaaagttat tattttttta cataaatctt caaaaaaaaa tatcttctaa
     3541 caatattttc attattaaat attgttttag aagatatttt ttaatataac ttatattttt
     3601 tataatgtta gggtctaatt tgtattttta tttcttctta atacgacaag tcctacaata
     3661 gctcctataa ctaaaagtat ttctccaata attgtagtac tatcccctgt ttttggtggt
     3721 actggtggat ttaccaaagt atcatcttta gctttattat ttttttcttc tgacactgat
     3781 ggctttacca cttctggtat tggaacaatc tgtttaacag gagtttctat cacctcttct
     3841 gatggtggta ctggtggatt caatatctct ggtattggag gatttattat atcttctgac
     3901 ggtggtactg gtggatttat aactatagta tcctctgtat cagatggatt tgatggtttc
     3961 cttggtttat ctgtgcttgg tggcactggt ggatttacta ttggttcatc tgtatttggc
     4021 ggtacaggtg gatttactgg tggattttct tgtcttatgt tttctatatt tccgatatct
     4081 actattctac caatatcttt attaccaatt tcaaccttaa ttttatcctt cagtttttca
     4141 tatccacttg gagccatcac ttctactaaa taatatattc ctggtttttt tacactagat
     4201 tgaattacac catcttcatc actaactaaa tccttaacta ctatatttcc aaatgcatct
     4261 tctaaattga acttagcacc tgaaagtaca tttttatcta aagcatccac ttttttagct
     4321 tcaaaagcat aaccttgctt agtagtctct actcccggag gacttgaacc attaatctga
     4381 gccacatttt gtatagtctc cccaactttt acatttgtga agtctgcatc aaatgtaacc
     4441 cagctttgca caggttcccc ttcttttatc tgtttaaatt cgtatttatt tactaatctt
     4501 attgtatttt ttccatctcc agcttctttt ttaagttcaa gattatcaga atattttata
     4561 ttagatattt ttagagatgg gtttacttca tccaaaagat ttacttgtcc tgcatcataa
     4621 accccataag ttgacatatt gattttgtat ttaacaatct ggttatcact actatttaaa
     4681 acctttttat caacttcttt ttttgcgctt attaatccag aatttttctt aaagtttatc
     4741 tcatcatttt tatcaatttc actattattt gcatcaagga cagctctatt tatataacta
     4801 tcttcaatac taattacctt taatgtataa gctataaaaa actttgattc tacttcttct
     4861 ttactgagat taattgtaaa tccagttata actttatcta cttgcccact ttcatttctt
     4921 ttttcttcta ctttatatga aatattattg gcaatttttt ctttactcca aggagtccag
     4981 ctcccattca ttggaacttc tacaactcta aaatcatgcc catcctctcc tgaagtagtc
     5041 aatctcatac cttctggtat attatctgtt ataacaacat tacttattgg tgatttatca
     5101 tcatttattg aaatctgata gtttattact tgaccaactt gagtaatatt tgttgctcct
     5161 gaatgtcctt tagtaagaag ataattatct cctctatcaa atatacttac agtagcatca
     5221 tcttcttgta tcttcttacc atcagattca atcctagcta cattcttata tttattacca
     5281 tgttttgcat ttttgtcact gattctagcc ttatacttaa cagtatacga tttatctata
     5341 tttccaaaat taactgaaat agaattttta ctctgttcaa tattaaaatc actaacttct
     5401 tttccatcac ttgttactac acttacagaa ccatttatca atgtcaatcc ttcaggaagc
     5461 tcatcataca aaataatatc ttttaactct tcctgctttt catttacttg tatactgtaa
     5521 tttatatctt tatttttaac tggcataaat acagtagttg attttcttcc ttcaacaagc
     5581 tttttaagtg ttcctttagc atctggaaca tccgttttat ctccaggttt tatttctaca
     5641 tctatatctc cacttccact ttctattttt ccagtggttt caatatgtat ttttgcatta
     5701 gtatcttcat caaccttttt aacatatgct tccaaaccta ttttaccttg cattacactt
     5761 tggccattag gtaattctct aaatgtaagt gtatactctc cattactttc tgttttatca
     5821 aagcattcac ttgtataatc taaattaact ttattgaata catctggcac cttaaaagtt
     5881 attgaatctc ctgcttgtat tccttttgat gttggagtat aagttaaatt aaatgatatt
     5941 tttttcattg attccgtttc tattgtattg ttattaattt ttacaacaaa tccatagtct
     6001 tcattaagtt cttcttctaa aactttcaat aaattttcat cttctaatag attatcttcc
     6061 tctggagttt tttctgtatt tttattgtct agtggttttt catcttctaa agcttgttct
     6121 gactttttat tatctgttga cttatcttct tctggtattt gctctggctt tttattatct
     6181 attgactttt ctccttctgg agtttgctct ggctttttat tatctattgg cttttctcct
     6241 tctggagttt gctctggctt tttattatct gttgacttat cttcttctga agtctgttct
     6301 ggttttttat tatctgttga attatcttcc tctggggtct gttctggttt tttattatct
     6361 gttgccttat ctttctctgg agtctgttct ggttttttat tgtcgctttc cttctcctct
     6421 ccagttgatg aattagttga accactttga atctgactat tctctcctaa ttctgaagca
     6481 taagatacag ttggatatat tgtagacacc accatactta aaatcataat taaactaatt
     6541 aataatgcct ttttatttcc tttcttcata taaattttcc ccctctaggt tatctatagt
     6601 ttataatctt ccattaatat cgtatataaa ttaattttta ctaaaaaatt atataaaaaa
     6661 agctatgaaa ataaataaat ttatattcat agccggttgc accactgact cagtatatcc
     6721 ataaggtgcc caataataga atatacttca acatctctat aatatttcat aaaaacaatt
     6781 aaaattgctt tatttatatt aaaaatacaa ttatatactt ctattatatt atgcctctat
     6841 actattgtct atctttttta cattttttac cttttatgag attaattaat aattaaataa
     6901 aatccttatc tacaacaatt acacaattat attcaggatt tatctcctta actaattttg
     6961 ttatatcttc ttttaagtca tcctctgaca tgtctttgga aaattcggaa ggattaacta
     7021 ctatatcaaa tattaaattc ttcttgcttc cttttccaac tattctaaaa tcatgcatag
     7081 attttacaag actatttttc ttaagtattt cttcaagttc ttttttggct gacataactt
     7141 ctttatcttc aagacatatt gggtccatgt gaattactag atatatatct aatttttcag
     7201 atatttctct ctctgcagta tctataattt catgtatttc cattatatct atgttggatg
     7261 gaatttctgc atgtatagat gcaatacatc taccaactcc atagttatga ataattaaat
     7321 catgtgtacc tattatatat ttataagata acaatagctc tataatagaa tttaccattt
     7381 cttcatccgg tgcttctcct aaaagaggat ttatagtatc ttttacaaga gagaaaccag
     7441 cataaagtat cactaatgat actacgatac caacatatcc atctatagga aaattagtaa
     7501 atctagctac aataaatgat ataactacac aacttgaagt aaatacatct cctaaagcat
     7561 caagtgaaac tgcttttaag gctgaagaat caattttatt tcccacaaat ttattaaaac
     7621 gactcaacca gattttaatc attatagata caattaaaag tataagtggt attacttcaa
     7681 attttacagg aattggattt actatccttt ctatagatga ctttacaaat tgaagtccaa
     7741 ccaacatcac cataaatgct actattaatg ctgataaata ctctattctt ccatgaccaa
     7801 aaggatgttc tttgtcagct ggcttacttg ctaatttaaa acctatcata gttattgctg
     7861 aagatgccat atcagaaaga ttattaaatg catcagccat tatagctata ctagatgtga
     7921 acattcctat gaataccttt atcacaaata aaagtaaatt ggacaatata cctactacac
     7981 cagctacata accatactta tttctaacat ctgtattttg aacgttttcg ctatctctga
     8041 taaatgtttt gactaggatt ttggaaaaca tgaaacttac ctccctgccc tttattttat
     8101 taacaaattt tataatctat atctttattt atgataacat aatttatata taaaattctt
     8161 aaaaatcact ctacttcagt aaaatattat tctatatatc tatttagata caaattttat
     8221 atttcgtaaa gtttttatgg cttagtgtct gtttaataga ttctatataa gtttttacaa
     8281 agattattta ttatgataat atcatttttc ccaagtatta cttagaatat gatttttttg
     8341 ttttgtcttt acatatttta aatttatact atatttatgt ttttataacc ttatgataca
     8401 ctataactat aagattttaa acggaaagga atgaaataaa tcatgttttt tatcagtgat
     8461 atttacacaa aatcacctat taaatttgat actccattgc aaaaagaggt atacaaaata
     8521 ttacaaaagt tagatattga ctttgaacgt gttgacacag atgaagcaat tacaatggaa
     8581 gattgtgttc agattaataa aaaattaaat atgaaaatgg taatataaaa caacattaaa
     8641 caaacaaaaa ccgcaaagtt acaatatata gtactttgcg gtttcataag aattaatttt
     8701 tactattaga gttcatactt ctactatttt tcattgttct aagtattagc tttacaaatt
     8761 catttgacaa taatatagtg gatgcaaata gtattatttt tatccacata tcaaagtcca
     8821 aagaaacaga attaaagaaa ctctggaaca cttgtgtaaa tattatttga attatccctg
     8881 taactactat tatttgcaat gccagtttat ttttaaagaa atttggtatt gtactattta
     8941 aaccaaattc tctacaattt aatgcattaa aaagggcact gaacgcaaat aaagaaaata
     9001 taacagtacc ttgttcttct gatgtagctc ccaatatatt aaaagctgat tgagtaaata
     9061 caattgtaat tataagaata gcatttataa taatatttac aaacatagac ctagctatta
     9121 ttccagaatg tctatttatt ggttttcttt tcaatacata atctctaaca ggttctaatc
     9181 ctaatgctag ggctggagga ccatccatta ttatatttac ccataataat tgtattgtag
     9241 taaatggcat atccttacca gtaagttgag atattatagc aattataaat gctactatat
     9301 ttacagtaag ctgaaactgt ataaatcttt gaaagttctc atatatacct cttccccatt
     9361 ttattccctc tactatagta ctaaagctat catcagtcaa tattatatct gctgcatttt
     9421 tagatacttc tgttcctgaa atacccattg caattccaac atctgcttga ctaagagctg
     9481 gggcatcatt tataccatca cctgttacag ctacaacttc gccacttttt tgaagcgctg
     9541 aaactattct catttttgta tctggtttac ttcttgcaac aatagatatt cctttaattt
     9601 cttctctcag ttctttatct gtcaatacat ctatatatgt tgcttcaact gcctttttcc
     9661 catcatttaa taatcctaat tcatttccaa tagccgttgc tgtatttata ttatctcctg
     9721 taagcatttt tacatctact cctgcattaa atgctttatc aatggaatct ttgacccctt
     9781 ctctcaatgg gtccactatt cctacaaatc cactaaaaac taaattatct tccttcatat
     9841 aacttcttga atttcctatt aaattcaatt caccttctga agtcattgca acttctgttt
     9901 tagaattact cattttttta taagcaaatc ctaatgttct catagattta atttgtaact
     9961 tcttaatctc atctaaaata cttttttcta cttttggagt tattggaaca atattttttc
    10021 cttgttgtac ataagaacat ttttttaata atacttctgg agcacctttt gaaagtaata
    10081 tatctgaatc ctcttgacga attaatgttg acatcttctt ttcttctgaa ctaaatggta
    10141 tttgtgatat caaatacgtt tgttttctca tttcattata gttcttatca ttatgatata
    10201 aaagtaatgc acactctgtt gcacttccta tatatttaaa tgaattatct tctttttcta
    10261 tatctgcagt tgaatttact atacagtttt gttcaaaata gctgttactt tgatactctc
    10321 ctccacttat atatttacca tcaacatatg ctacctctac catcattttg ttttgtgtta
    10381 aagtccctgt tttgtcggaa catattacag aaacagaacc tatagtttca caggcttctt
    10441 tctttgttac taatgcattt atctttgcca tcttttgcat tgttatagct aaggtaatat
    10501 ttatcatagt tggcaaacct tctggaacag ttgcaactat taatgcaata caaaccatat
    10561 atgcattttt agcaggttct aatgattgta agaaagataa tatactgctt gtatctaaaa
    10621 ttaacatacc ttttctctgc atctgtataa tcataaacat acacaataaa gtagctatta
    10681 cgcctgatat tcctgctatt ttagcaccca acttccctaa tttaatttga agtggagttt
    10741 gctggtcttc atttgataaa ttctgtgcta tcttacccat ctctgtctta tcaccagtat
    10801 gagtgacaac cattatgccc ctaccatatg ctacaagagt ccctccaaat accatattga
    10861 cttgctttgc aggtattgca tcctgttcga ttatttctgt tttagaataa actacttcca
    10921 tatctaatac agcatcagca ttcttagcta catcatcaga ttctcctgtt agcatatctt
    10981 ctctaagttt caaatttata gactgtataa gtcttccatc tgctggtatc atatctccag
    11041 tttctatata tacaatatct cctggtacta aatcattttt tgatatttta attatctttc
    11101 catttctaag tacttttact tctatatttt ctgttaattt tgacagggcc tgagctgctt
    11161 ttttagattt tccttcagtt attatgccta tagaaattcc aataaaaata gcccctaaaa
    11221 tacctaatgc atcatgtaat tctcccacaa aagaacttat tactgctgct cctatgagaa
    11281 ttaatatcat aggttctgtc aaactttctc ccaactcgtc ccaaaatgtt ctaccttctt
    11341 ttatagtaaa ttcatttaat ccatatctaa gcttcctttc ttctacttca ttatcatcaa
    11401 gtccaatttc tggatttgtt tttaagtatt ttaatacttc tttggtaggt ttattataat
    11461 acctcataac tttatccccc ttgttaatta tctataagga attatatgct tatgatagct
    11521 tgtatattac ttttcaaaaa aaataaggtg ttgattaatc aacaccttat agtggttata
    11581 tattattaaa ttttatgtta caggctttat aaattgtgag ctatacaaat caaaatattt
    11641 aatcttcaaa atacccttct ggatataatg ggaacttaga tataagttct tgtgcctctg
    11701 ctttacattc atctaatact tctttattat caatattttc tgctacctta ttcattattc
    11761 ctgctatctg tataacttct ttttctttca gacctctttg agatattgat gtaagaccta
    11821 tacgaacacc actagttaca aatggtgatt gtgggtcgtc aggtataaca tttttattaa
    11881 ctgttattcc tacatattca agagcttttt caaattgttt tcctgtaaga ttcttacttc
    11941 ttaagtcaac cattactata tgattatcag tacctccact tacaatacta aaaccatgtg
    12001 aaatcagttc agaagctaga gtctgagcat ttactacaac ttgctccatt atctttttaa
    12061 atttatctgt acttaaatat aatagtgaaa aggcttttgc tgcaactgta ttaagatgta
    12121 tagaccctaa tgttcctgga aaaacacctt tatcgagttt ctttgcatgt tctgctttac
    12181 aaagtacaat accacttctt ggtccacaga aagtctttgt cgtagatgaa gaaacaaaat
    12241 ctgcataagg tacaggacta ggtattactt tcgctgcgac taatcctgca acatgtgcca
    12301 tatctaccat aaagtaagct cctacttctt ttgcaacctt tgatattctt tcataatcta
    12361 ttaatcttgg atatgaactt gcccctgcaa ttataagttt tggtctacat tcttttgcct
    12421 ttgcttcaag agcatcataa tctatacgtc cagtatttgg gtctacccca taaaattcat
    12481 atttataaat gtcatgtaaa aaattaactg cgctaccatg tgtcaagtgt ccaccttggt
    12541 caagacgcat acttaaaaca gtgtcatttg gttttaatat tgatgaatat acacaatagt
    12601 tagctgtaga acctgaatac ggttgtacgt ttacatgttc tgctccaaat acttcttttg
    12661 cacgttttat tgctagggtt tcaagtttat ctgcttcttc agaacctgct tgaaaacgag
    12721 cccctggata gccttcttct gttttatttg taaatacaca tccacttaat tcaagtactt
    12781 ctgttggtgc tgtactttca gaagctatca tctcaatatt gtgttcttgt ctaaccaact
    12841 catcagctac aatcttgtat aactctgggt ctgaaatttt tgttgttttt aacataataa
    12901 tcctcctctt aattaaagaa tatattttat tagtttaatc ttcattgata aatttattat
    12961 aatggtctat aaaacgtaag atattcctcc accaggtcct aatggtatgc caatacccat
    13021 gaatagtaac aatattatta tttctattaa tgtaaatacc actgagtatg gaatcatatt
    13081 cgatatgaca gtacctatac ctatatcttt atcatatctt ctggcaaagg ccaatataac
    13141 tggaaaatac ggaaaaagtg gacttattgg atttgttgaa gcatctccaa tacgataagc
    13201 tatttgagtt aatgctggat taaagtctag tagcataaac atgggaacaa atataggtgc
    13261 taaaatcgcc cattttgcag aagcacttcc tacaaataaa ttgataaatc cagatagcaa
    13321 tataaatcct attatcaatc catagctagg tatccccgac tctgctagta attttgctcc
    13381 tgttattgat aaaattgttc ctaagttact aagattaaat aaattaataa actgggatgc
    13441 tataaatgct aggactatat atccacccat atcactcata ctactaccca tcatagatac
    13501 taaatctttg tcacttttta tcttcttact tacttttcca tacactaatc ctggagttaa
    13561 aaatataata gtgattatag gaactatacc tttcattaaa ggtgcattac tagatagtat
    13621 agaaccagtc tctggatctt tcataaatgc attgtcacct atacataagg caactatcac
    13681 tactactaca actaacaatg ataataaagc gtattttata cccttatttt caagttcact
    13741 caattcctgt atatccttat catttgttga atcattatat tttgtaaaac gaggagctat
    13801 aaatttttca gttacaaatg ctcctgcaaa agttaaaact atcgctgata taaaagcaaa
    13861 ataaagattc attgctggat tgccttggta agatgggtcc atcatttgag ctgctggtat
    13921 tgtaaatcca gctaacaata catctgtagt actaagcata acatttgctg caaatccccc
    13981 tgcaacacct gcaaaagctg caaacatacc tacaagagga tgtcttccaa ttcctaaaaa
    14041 cacaagtgct gcaagtggtg gtaatattat aaatcctaca tctgctgctg tatgtgataa
    14101 catacctgca aatacgattg ctacagttac aagtttttgt ggaactttac ttataccctt
    14161 tttcattaac acttccataa atccagattt ctcagcaaca cctgctccaa gcattgtaac
    14221 cagtactaat cctaaaggag caaatgactg gaagtttgta actatgctac ctaaatatga
    14281 ttgaatctgc tctttctcta gtagatttac tacctttaca acttcttttg tagaaggatg
    14341 tatttcttct actcctaaat tagatataac tgctgacaat attacaacaa ttatacatag
    14401 taataaaaat atggttactg ggtctggaag tttatttcct gctctctcta caacatttag
    14461 tgccctcatc aataatccac tatctttgtt tttttcctgt gaattaattt tcctcaaact
    14521 aataccccct cataaacttt atctccagaa tgctctatct taaattttat tttttaattt
    14581 atccatataa tttataacag actgacatag tacatttgta cctaccaata tagaattttt
    14641 atcagcttta aatttaggat tatgccactg atatatacaa tcttgttctt tattgcctac
    14701 ccctaaccag taaaaaaatc ctggggcctt ttccataaat atagaaaaat cttctcctcc
    14761 tgtagaaggt attggatcaa ctatagcatc ttctgaatac aaatcacaca cagacttaca
    14821 agctattgtg tacatctctt tactatttat aacagctggt aaatcaaata aataatctag
    14881 ctttgcagtt gcttgatatc cacgagctat ctcacaaact atattttcta aattttctga
    14941 tattttcttt cgtacttcat tactaaagct tctacatgta cctgtcatat ctaccttttc
    15001 tgaaattaca ttatttgcag tccctccatt tatagtacct attgatataa cagctgattc
    15061 taatggactt atgtttctgc ttactatggt ttgaataccc attataattg cacttgatgc
    15121 tactataggg tctattgttc tgtttggtat acctccgtga cctccaaatc catttacttc
    15181 tatcttaata gtgtctactg ctgccattaa cccacctaac ttaacaccta tttttccaca
    15241 aggtatatct ggatgattat gaagtccaaa tatagcatca acttttggat tttccattac
    15301 tcctcgttcc actagcatct tcgcaccttt attgacttct tcggctggtt gaaatataaa
    15361 ctttacattt ccatgaagac tatctctaat atgagacaat acatatgcac atcctacaag
    15421 gcaagatgtg tgtatatcat gcccacatgc atgcattata ccatcatatc tggatttata
    15481 ctcaatatca acctcttcat atattggcaa tgcatcaata tctcctctca gtgctactgt
    15541 gggaccatca tacttccctt ttaataatcc aacaactcct gtttccattc ctaaatctaa
    15601 tatttctatg tctaaactta ctaataaatc ttttatagct tttgtagtcc taaattcttt
    15661 aaaacttaat tcggggtgca tatgaatatc tgtaaaaaaa tctaaaatat cttctttata
    15721 cttttctgag attaaaataa gttcatgcat actaacacct ctccaaaatt ttattttttt
    15781 attttttata atttaactac aaattatata agttaataat ctttataaag atatttttta
    15841 taaatttaat tt
//
LOCUS       DAEPOG010000089        15846 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEPOG010000089 DAEPOG010000000
VERSION     DAEPOG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.089"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            146..1336
                     /gene="dltD"
                     /locus_tag="KR432_002259"
     CDS             146..1336
                     /gene="dltD"
                     /locus_tag="KR432_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBH0640789.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            1457..2971
                     /locus_tag="KR432_002260"
     CDS             1457..2971
                     /locus_tag="KR432_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBH0640790.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            2971..4119
                     /gene="dltB"
                     /locus_tag="KR432_002261"
     CDS             2971..4119
                     /gene="dltB"
                     /locus_tag="KR432_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBH0640791.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            4138..4368
                     /gene="dltC"
                     /locus_tag="KR432_002262"
     CDS             4138..4368
                     /gene="dltC"
                     /locus_tag="KR432_002262"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBH0640792.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            4585..5370
                     /locus_tag="KR432_002263"
     CDS             4585..5370
                     /locus_tag="KR432_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0640793.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            5649..7559
                     /gene="phnW"
                     /locus_tag="KR432_002264"
     CDS             5649..7559
                     /gene="phnW"
                     /locus_tag="KR432_002264"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBH0640794.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            7916..8737
                     /locus_tag="KR432_002265"
     CDS             7916..8737
                     /locus_tag="KR432_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640795.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            8778..9545
                     /locus_tag="KR432_002266"
     CDS             8778..9545
                     /locus_tag="KR432_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBH0640796.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            9553..10173
                     /locus_tag="KR432_002267"
     CDS             9553..10173
                     /locus_tag="KR432_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBH0640797.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(10473..11060)
                     /locus_tag="KR432_002268"
     CDS             complement(10473..11060)
                     /locus_tag="KR432_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0640798.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(11146..11583)
                     /locus_tag="KR432_002269"
     CDS             complement(11146..11583)
                     /locus_tag="KR432_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0640799.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            11687..12685
                     /locus_tag="KR432_002270"
     CDS             11687..12685
                     /locus_tag="KR432_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBH0640800.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(12805..13731)
                     /locus_tag="KR432_002271"
     CDS             complement(12805..13731)
                     /locus_tag="KR432_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0640801.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            14117..15721
                     /locus_tag="KR432_002272"
     CDS             14117..15721
                     /locus_tag="KR432_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0640802.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         6267 a         1674 c         2730 g         5175 t
ORIGIN      
        1 tgtttttttc ttttttttta caattttgta acagtatcaa aaaaagtttt gacgatttta
       61 ttacaatttt gtagttgaat atacatatca aaactaatat gattaataat aacataaatt
      121 tgaatatttt tagaggagag aaaatatgag aaaattgata tactttatta caccttttat
      181 aataggcgta gtatttttat ttgggttaga caaatttctt gattctaaaa ctgatgaact
      241 tttgagggag aaaaatctac ttccaattat ggatgatact ttaagtgata taaaagataa
      301 aggggttaca gcaaataatc attttttaag agaaaaggat attatgattt tggggtcatc
      361 agaacttagt aattcaacaa aacaacatcc aaagtactat tttaatacaa ataggagtaa
      421 aaataaggtg tttgcaattg ggagagccta tactcagaca ttacaagatg ctgcaatatt
      481 aggaagtatg aatcctaata ttgataataa gaaagttgtc ttgttaattt ctatgcaatg
      541 gtttatggaa aaagacggag tcacaagtca ccattatcag tctagatttt caccaataca
      601 attttatcga tttttagata atccaaaaat atctaaacaa aataagattg aatatgcaaa
      661 aaaatcaagt aagctattat ggggaagtga tgagtataaa gcagaagcat tatatgctaa
      721 gctatatgaa cctaagacat ttcctgaaaa agctgaaaag gtgcttttag agccttattt
      781 tcaaggaaga aagtactgta tagctcttaa agaaaagggt attctttata aacgattaat
      841 taagctagat aaaaagagag caacaaaaag aaaaagacca ataaattggt catatgagag
      901 aaagaaagcc attgaagatg ctaaaaaaag agttggtaaa aatccactaa atattgataa
      961 gtactattat aaagagcatt ttaaagatgg tatagagcaa tacaaaggaa gagacaaaga
     1021 tgtaaattta ttaacatcta aggagtttga aaattataaa ctgatgctaa atgtatgtac
     1081 tgacttaggt attaatccag ttgttgtatt aatcccaagt atggataaat tctataattt
     1141 gactggtatt tctgaaaaag aaagaaatca gtactacgat aaagcacaaa aaatagcaga
     1201 aagcaaaggc tttgaagttc taaaccttaa agataaaggg tcagataagt attatttaag
     1261 ggatgtaatg catcttggta cgaaaggatg ggtagatgtc tgtgagagat tatttaaaat
     1321 atttaaagaa caataaagat ttgttaacta gtatttgttt aactatggtt ataatattgg
     1381 cgataggagt tctaacattt actccattta aagaattgcc atttatttat aacggatttt
     1441 aaaggagcat tcttatatga aaattattga aggaataaaa aaatattcaa atacagatag
     1501 aacagcacta atgtgtaatg gagataaact ttcttataaa gatttaaatg aatattcaga
     1561 tgctatatca gtatttctaa aggatgtata caaagaagag gatactccta tagtaattta
     1621 tggaaataaa gaaaacatga taatggcatg tatgatagga gctttaaaat caggtagagc
     1681 gtatgtgcct cttgatatta gttttccaat tgatagagtt tttgaagtca ctaaagagat
     1741 aaaaccaaaa gtaatattta attttagtga tgaaagaaat tttggagata tcaatgtaat
     1801 tgatatggat aaattaaatg atataataaa tgaatatcag ggaaaatcat tagataaaga
     1861 aaactgggta aaagatgatg aaaatgcata tatactattt acttcaggaa gtacaggaaa
     1921 gccaaaaggg gttcaaataa gttcaaacaa tttagatagc ttttcagatt ggataagccc
     1981 atatctaaac atagatggaa gtgaaaaagt aataatgaac caaccagcat attcttttga
     2041 cctttcagtt acaacaatat accctggtct tatccatgga gctacattat tttcaatatc
     2101 aaaagatgta ttagcagact ataaagaact atttagacaa tttagtatct cagatatagc
     2161 tgtatgggtg tcaactcctt cttttgcagg agtatgtata actgaaaaag agtttaatag
     2221 taaaatgctt cctaaccttg agtcaatgat attcatagga gaagcacttt caaaaaatct
     2281 tactaaagaa cttatgagta gatttccaaa cacaagaatt atcaatggat atggaccaac
     2341 cgaggctact gttggtgtaa gtgtaaatga tatgactcaa aaagctatag atgatgaaaa
     2401 aagtcttcca gttggatacc ctatgtcaaa ttgtaaaata aaaatacttg atgaagatgg
     2461 aaatgaactt aaagaaaatg aaaagggtga aattataata attggaccat cagtttctaa
     2521 aggttacttc aataataaag aaaagactga tgaagtattt ttctatgatg aaatagatgg
     2581 tgtaaagtgg agagcttata aaactggaga tatgggatat cttttagatg gaaatatata
     2641 ttattgtgga agaaaagatt ttcagattaa attaaatgga tttagaattg aaatagaaga
     2701 tatagaaaat aatttgagaa aagtacataa tgtaaaaaat gcagtagttt taccagtata
     2761 taaggacgaa aaaattgctt acttaaaagg tattgttgag ttaaatgaaa agaatgattt
     2821 aagtaatatt aaaaatggga tgataataaa aaaggaactt ggaaagtata taccttcata
     2881 tatgattcca agaaatattt caataatctc tgagtttcca acaaatatca atggtaaaat
     2941 agacagaaag aaacttatgg aggaaattta atgatttttt cacagtatgg agattatttt
     3001 tatctttata tattattatt aacatcaata ccagcagtaa ttttaggtct aatgggcaag
     3061 aatataaagt attatggtat gttggcaagt ttgtttatga tattcttaat agtaggaatt
     3121 gatgtccagc taaagtattt ggtaatattt attattcttg aagtaataat agttaaagga
     3181 tatgaatatg taagaagaaa aactaaaaat aaatacatat attggggatt cttgtttgca
     3241 tcaatgctcc caataataat aaataaaata tctccagtta cttcttttgg aataattgga
     3301 tttattggaa tatcatactt aaatttcaga actatacaaa tggttattga aatttatgat
     3361 ggagctataa aagaagttaa aatttcaaag atgctttatt ttatgttatt ttttccaact
     3421 ttaagttctg gacctattga taggtcaaga agatttgaac aggacttgga aaaacacatc
     3481 tctagaaaag agtatataga agaatactta ctgccaggaa taaaaaatat tgtcatggga
     3541 gtaggttata agtttgttat agcatttttg ataaatacat attgggtatc aagaatacct
     3601 aaagatatga gttttataaa tattttaagt tatatgtacg catatagttt atacctgttc
     3661 tttgattttg caggatatag tttatttgct ataggtacag ggtacatatt tggaatacaa
     3721 gtacctataa actttgacaa gccatttatc agtaaagata tgaaagagtt ctggacaaga
     3781 tggcatataa gtctttcaag atggtttgga gattatattt tttctagatt tgttatgtca
     3841 tcaatgagaa agaaaagatt taagaaaagg actactgctg ctcatgtggc tcagatgata
     3901 accatgataa caatgggatt ttggcatggt ctaacttggt attatgttgc ttatggtgta
     3961 taccaaggat tggcacttgt gttaactgat atatatcagc gaaaatctaa attttataaa
     4021 aaacataaaa aagataaatg gttcgagaga gtgcagattt tcattacatt ccatattgtg
     4081 tgttttggat tgctgatatt ctcaggttat ttagcttaat tttaaggagg aaaatttatg
     4141 caagaaacag ttatagaaat atttgaagat gtattaggga ctgatgagat aagagaagat
     4201 ttagacctta atttatttga gacggaactt ttagactcat tagctataat tgaagtttta
     4261 cttgaaattg aaaatagatt agggatagag cttcaaccaa ctgatttaga gagaaaagat
     4321 atgtctactg taaataattt agtaaaattc ttggagacaa gaaaataaat tagtagttag
     4381 actttaaata ttaatttata aaataaaatt ttacaaagtc catctgatac taatatttga
     4441 tggacttttt taatgataat acaaagtctt ttgaaaataa ctttaattaa gtaaaaaatt
     4501 taatacataa atatgatttc ataaatctta ggtatcattg taatattata ggagaaaaat
     4561 atattatatt taggaggaaa atgaatgtct ttaaattcga tagatagaaa aaaagaaata
     4621 cttaaattat tagattcaaa agggaaggta aatactaaag aattagtaaa aagattagaa
     4681 gtatcatcag aaacaataag aagatatttg gaagaattag aaaatgaaaa taaattaaaa
     4741 aaggtatatg gtggtgctat aaaaataaat tatggagtga ttgaaccaga acatataaag
     4801 agaaacaata ctaacctaga taaaaaacaa agtattgcta aatacgcagc taagtttgta
     4861 aatgacaatg agtcaattat catagatgaa ggtactacca atttgcagat tgtagattat
     4921 ataatatctg taaacaattt aaaaattatt acaaactctt atccagttct atcaaaattg
     4981 atttcttatg agaatcaagg tttgtttgat ggagaagtta tatttattgg tggaaaagta
     5041 aatttaaaac accaaaggac agcatgtcca atgtcaatag acactatgaa aaacttatac
     5101 gtagataaag cttttatatc tagtgaaggt atacttgata gttttggaat tagtagtata
     5161 gaccaaaata aggcattgtt gtcaaaagaa tatataaaaa attcaaagga aagttttgta
     5221 ttatgtgata gttctaaaat aggaattgct agtatgtata agatagcaga tttagaggaa
     5281 ttagattttg ttatttgtga tgtagaacct ccgaatgaat ggaaggataa gttaaatgag
     5341 ataaatataa catggataga agcaaaataa acccttagtt ttatataata caactatgct
     5401 taatacaact aaattcataa aatatagtca gttcatagat taagattaga aagtaaattt
     5461 tagattgtaa cttaggattt tatggaaata tgatataaaa taaaaacaat taaatacaac
     5521 taaaaacaga aatttaatat atatttaacc taaatatgat gtataattaa catatgaaag
     5581 ttatacttta aaagtaaaaa caattaaaaa cacaaaaatt ctaaaatgaa tctatggagg
     5641 ctataaatat gaaaaagata tatggagaaa aaataaaagc agtagtattc gattgggctg
     5701 gaacaacagt agattatgga tgttttgcac ctcttaatgt atttatagag atatttaaaa
     5761 gaagaggtat agatgtaaca atggaagaag caagaaaacc tatgggaaaa ttaaaaatag
     5821 accatataag agaaatgtgt gaaatggata gaataaaaaa tctttggtca gataagtttg
     5881 gtaaagtgcc aactgaagat gatgtaaatg aactttatgc tgaatttgag ccaatgttat
     5941 ttgagacatt agaagagtat acgactccaa ttcctcatgt tgttgaaact attgaaaagt
     6001 tgagaaaaaa tggattaaaa ataggttcta caacgggtta tacaagagag atgatgaaca
     6061 tagttgaacc aaatgcagca aagaaaggat actcacccga ttttcttgta acaccatcag
     6121 aagtatctca aggaagaccg tatccttgga tgtgctataa aaatgctgaa gctttagggg
     6181 tgtctcctat gagttctatg gtaaaagtag gagatacaat aagtgatgta aaagaaggcg
     6241 taaatgcagg aatgtggagt gttgctgtaa taaaaggttc aagtgagtta gggcttacac
     6301 aagaagaagt agagaacatg gataaggaag aattaaaagc taagatgagt atagtttcaa
     6361 agaaatttaa agaggcaggt gcacattttg taatagaaac tatggctgag ttagaagata
     6421 tactaataaa aatagagaat gaaactatta aatcagattt tgtaccagaa aatgattaca
     6481 tattacttac accaggacca ctatctacta ctaaatcagt aagagcatct atgttaaaag
     6541 attggtgtac ttgggatgtt gaatataata acctagtaca agatgttaga agaagattag
     6601 tatctctagc aacccaaaat acagataaat acacaagtgt attaatgcaa ggaagtggga
     6661 ctttttcagt agaagctata ataggaagta ctatttcaaa agatggtaaa cttcttgtaa
     6721 ttgcaaatgg agcatatgga aagagaatga aagatatatg taattatctt aacatagaat
     6781 ttgttgactg cacatttaaa gatatagaag cagtggattt aaatgtagta gaaaatttat
     6841 taaaagaaaa caaggatata acacatatat caatggttca ctgtgaaact actacaggta
     6901 gattaaatcc tatacaagag gtagggaaac ttgcaaaaga atataacaaa atttatatag
     6961 ttgatgctat gagtagtttt gggggaattg agattgatgt tgaagatttt aatatagatt
     7021 ttttagttag tagttctaac aaatgtatac aaggagtacc tggatttggt tttataatag
     7081 ctaataaaga gaaattatct aaatgtaaag gtatagctaa atcattatct cttgatgtgt
     7141 atgcgcaatg ggagactatg gagaaaaata atggtaaatg gagatttact tcacctacac
     7201 atgttgtaag agctttttat caagctttat tagaattgga agaagaaggt tctgttgaaa
     7261 aaagatatgc tagatataaa gaaaatcagt ttacaatagc ttcaagatta aaatctttag
     7321 gatttgatac tttggtgaat gataatgctc aatcacctgt aataacaact ttcttatatc
     7381 ctaaaaatgc aaagtttgaa tttatggaat tttatactta tttaaaagat aatggatttg
     7441 ttatctatcc aggtaaattg actgatatag atacatttag aataggtagc ataggtgaag
     7501 tttatccaac tgatatggag agactagctg atgtaataga aaaatttatc aatagataga
     7561 agaagatagt gtcttttagg ttacagaata gtgcggagag cctactcctt taggtgtgaa
     7621 atgatagcac aaacagtgta taaaaagtat tgacagtaga atttaaagac ttatatttta
     7681 gaagtttata caatgaatat ataaagattg agtaataggg atatatttta tttaaaccac
     7741 aatctaagaa attagatttc tagtttaaat aaagcatttc cctattattt ttatgcctat
     7801 ttatagaaaa taaatgatag ttgctttatt aagtattatt atcagacttg acaacgtaac
     7861 aatgttttgt tatattaggt ttaaagataa aatacggtaa agaaaggata tcattatgaa
     7921 tgaagaatta agttcactaa gatttgatgg tgttggaaaa tgtaaaggtg gaatttttga
     7981 taatgttaat atatcaggaa caggcaaaat agaaggaagt gtaaaatgta gtaaatttga
     8041 ttcttctggt tcagcaaaag tttttgggac aacagaatgt aatgaatttt ttacatctgg
     8101 tttaagtaaa gtagaaggaa acatactggc atcaaaagtt gagatatcgg gacttcttag
     8161 atgtacagga aatattaatt ctgttgaaat tgatactagt ggaattatta atgtagaagg
     8221 aagtataaaa tcggatatta ttcatggaga aggatattta aaagcaaatg agaatataga
     8281 ttgtgaaaat atcaatttaa gtggaatctt ggagtgtaat ggatttttga attgtgaaga
     8341 agttaatatt agtttaaatg gaacttctaa ttttaatgaa gttggagcaa gtagtataaa
     8401 tataaaaaag gggcatggag attattgcaa aaaatttcgt atgctagaaa aaaagcatat
     8461 caactttgaa aaagtgtctt caaatttaat aagtttaggt ctattatcaa ttagaaagta
     8521 tacagaaagt aaattgtttg caaatattat agaagcagat gaaataagct tagaaaatag
     8581 tgaagtcaaa gttgtaagag gaaaaaatat taaaataggc aagggatgtt atgtagaaag
     8641 tttggagtac agtgaaagta tagagattga tgaagattca atagttggtg aaataaagaa
     8701 tataagtgaa aaaataaaat taaagaagga taagtaataa ataggtttaa aaataaaatt
     8761 taaaatgagg taataatatg gaaaaagggt ctataaaaat acttggagaa ggctttataa
     8821 gtagtggtga gtatgaaaat ataaaaatcg tgggaagttc taaatcaaaa ggaaatgtta
     8881 aaagtaacga agtaaaagtc cttggagatg caaaatttga aggtgacatc aatataaaaa
     8941 attttgttgt taacggaaaa actattataa gaggcaatct taaagcagag cgtgtaagag
     9001 tgaatggtca acttaatata gagggagatg cagaaataga tgacttagtt ataactgctc
     9061 aacagaaagt ggatggcaaa cttaaatgta ataatgttac aataagagga caattaagtg
     9121 taaatgaaaa tatgtatgca caagatataa aaatctatgg cgaaatgaga aataaatcaa
     9181 atatagaatg tgaagatata aaggtatatg gagtaataaa atgtgatggt cttttaaatg
     9241 gagaaaatat atatgtttat tcaggaggag gttcttattg taaagagata ggggcaacta
     9301 atataaaaat aggaagaagc aaagatgctt tttcattaat taaaatcatt aatatttttt
     9361 caggaaagtt taaatgtaat ttgatagagg gagatactat tgaattagaa gatgcatcaa
     9421 taaaagatat acgaggcaaa agcatatctc tagtagataa ttgtgacgtt ggaaatattg
     9481 aatacagtga taatttatca gtttcagata attctactgt aaaaaacagt ataaaggtgg
     9541 gctagaaaag ttatggaaga attaatatct aagaaagatt tattgagcaa aactaatata
     9601 tcttatggtc aattatatag atggaaaaga aaaaacataa taccagagga atggtttata
     9661 aaaaaatcag cttttacagg tcaagagact ttctttccaa gagataaaat actagaaaga
     9721 atagagttaa tattgtctat gaaagaagag acttctcttg atgatattgc aaatatgttt
     9781 atgaaaaagg atattaataa ggaatttgac attgatttta ttctcagtaa agaagttata
     9841 tgtaattata ccaaagaaat atttcaaaat ttatataaaa aagagcaaag cataggtaaa
     9901 aaagaactgc ttatattgag cataatagag aagtttttag ttaaatctat aataactttt
     9961 gaagagttaa aattgatagt gaatataata gaagaaaatt ttacatcaat atacaatgaa
    10021 agtggaaaga tttatttgtt tagaaagttt ggagttccat tcgtagtagg atgtctggat
    10081 tataaaaaag taaagtttga aaaagatgta gttaagatta ttgagattga cttgacaaaa
    10141 gaaataaatg atataagtat aaagttatca tagtgtatta tcttatataa tgtattataa
    10201 taatctactt aaaatacact ttatttaaat atttagttta aagataagat tttatgtgag
    10261 tatgtaaaag ttaaagatag atttaagtag gttataggga ttagtaaata ataaaatgaa
    10321 ccatgatgaa ttactataaa ttgtatttaa actacatttt ctacttatag taatatcaat
    10381 ggttcatttt attgttatta agataataca gtatttataa aaatacttga tataaatata
    10441 gtaaattgtt taaatagaat tattgcctta gtttattcaa ataatgagtt tttaaaatct
    10501 aagaaatatc cttgtggatg aagacataca ggacaagctt ttggagcttc tttacctata
    10561 tgtatatggc cacaatgtgt acatatccat tgtatatctt catttcttct gaataaagta
    10621 ccttcttcta aatcagtagc atatctacca aatctatcac catgaacttt ttctatttca
    10681 gctatatttt caaataactt agctatagat tcaaatcctt cttcacgtgc tgtttttcca
    10741 aagtttttat atacatcatc ccactcttga agctcattat gttgtgcagc tttaaggtgt
    10801 tgtgctgtct tgtcatataa atctacaggg tatgctcctg atatacttat attttcacca
    10861 gcaaattctt ttaatttaga aaagaattgt tttgcatgtg atttttcttg gtcagcagtg
    10921 taattaaata aagactctat tatatataat ccttcttttt ttgctacaga tgcagaaata
    10981 ttgtatctat ttctagcttg actctcacct gcaaaagctc ttaataaatt ttctttagtt
    11041 ttactatctt ttaaattcat attctacctc tctatcataa taaattttta caatatttga
    11101 ttctttttat ttattattcc aataggagat ataattatgc tcaatttagt cagcattttt
    11161 aaaattatca ttaatttttt ttaaaattct aagaaattct tgtttttcat tttctgtaat
    11221 attatggtat gctgtctctt taacttgtgt tgagatgcta ttaaaagtat cttctataag
    11281 cagtgccttt tttgtaagct ttatatacgt gatacgtttg tcatttgtac atttttgtct
    11341 ttctaaatat cctaggttta taagtttatt aaccaacact gtaacagtgg atttatcttt
    11401 tcctatttta gctgcaattt ctttcattgt aagtattcca ttattttcat aaagtgcagt
    11461 taaaatattt ccatgagtag gtattaactc acctaaacca tttttactga gtttattttc
    11521 aatgaattta atcatactag cctttgtttt acttatgaaa tgtacaatat atttatcttc
    11581 cataaattat tctccaatct ttaatctatt taaattagtt tgacataaaa ctatttataa
    11641 actataatta gtttaacata aaactaaatg tattgggagg aaatttatga ataaaaaatt
    11701 taaatcacta tatattacag aagaagaagg aaattttaga agaaatatag ttgaaagaga
    11761 agtagataca ttgccagaag gcgaagttct aataaaagta aaatattctt ctctaaatta
    11821 caaggatgca ctttctgcaa atggtaataa gggtgttacg agaaagtatc ctcatacacc
    11881 aggaattgat gcatctggta tagtagaatc tagtagttgt gatgatttta aaattggaga
    11941 agaagtttta gtaacaggtt atgacttggg aatgaatact tctggtggtt tttcagaata
    12001 cataagagtt ccttctagtt gggtagttaa acttcctaaa aatctttcac taaaagaagc
    12061 aatggattat ggtacagcag ggtttacagc agctttatct gtgtataaat ttatagattc
    12121 tgtagacaag aatatgggag atgtattagt cactggtggt actggaggag ttgctgtaat
    12181 tgcaactaaa atattgatta aattaggtta ttcagttgta gtttcaacag gaaaattaga
    12241 agaacaaaaa gaagtattac taaacttagg tgttaaagat gttatacata ggtcagaagt
    12301 ggatgataac tcaggaagac ctctgcttag accaagatgg gctggagtaa tagatacagt
    12361 aggaggaaat actctagcaa cagcaataaa gacaactaat tattgtggag cagtaacaac
    12421 ttgtggtaat gcaggaggag ttgattttac aagttcagta tatccattta tcttaaaggg
    12481 aattacttta tatggtatag attctgtgga ttgcccaatg aacttaagat taaaaatatg
    12541 ggatttatta tcaaatgaat ggaaattaga tgatttagat gatatggtaa atgtagtatc
    12601 attggaagag ttaaatgata gtatagattc gataatgaca ggaaaacatg ttggaagaac
    12661 agtagttgat ttagaaaaaa tataggcgaa taaatttata attaggatta taggttttaa
    12721 gcaaaaatga gatacttgaa atttacttat gagtatctca tttttttatg taaaaaaatt
    12781 tatgtagatg tttgtgtaaa aagattattc atgaacaatt aattcaaata catctggtct
    12841 tgaatagtga ccttctgaat caaaatctag tctactaagt actatttttt ctaggtctaa
    12901 atcagctatt agcatttctt ctttattgaa aataggacct gctacatatt ttccaaaagg
    12961 gtcaactata caacttcctc ctggacacat aatttctggt tctgcatcta gctccttata
    13021 gtagttaagg tcagttggat acatattttt ttcaacatat tgattgcatc caattacaaa
    13081 acatcttcct tctaaagcaa tatgcttcat agttgcttgc cactcttctc ttgaatctgc
    13141 agtaggggct atgtaaagtt ttacaccctt agcataaaga actgttctag ctaatggcat
    13201 ataattttcc caacaaatca acgaacccat ttttccatat ggagtatcaa ctactgtaag
    13261 agtacttcca tctccttctc cccaaataca tctttctgtt cctgtaggtt taagttttct
    13321 gtgtttacca attagcttac cttctggaga gaaaaataag tttgtgcaat aaagggtaca
    13381 atttatatca ttaccatctc tttctgttat acccattgat agatatactc ctgcttcttg
    13441 tgctgctttt cctaataaat ctgttgtagc actaggaact ggtacagaat tatcatagta
    13501 tcttttccaa tcttctctac cttccattgt tctacttcca acaacaaatc caaatgaaag
    13561 acctcttgga tatgcaggta tgaatgcttc agggaaaact acgatatttg ccccttttct
    13621 accagcttct ttaattaatc ctaatgcttt ttcaacagtt ttttctaaat ccattatgac
    13681 tggtgacgcc tggacaacag caattcttac atttttattt tccttgctca taaaaaacct
    13741 cctaaaaaat atttaaattt tttgcattga atagattaca tgagatgtta tttgagaact
    13801 aagatactta tattgtgaat aataatgtaa ctgtatattt aaaataatac atcatgattt
    13861 aaaaaattac aattgtacag atataattga gtttagatat ttaaatttaa caacttataa
    13921 tattttttca attagtataa agcctattaa tttgtcttat ttacaatgaa tagtatttac
    13981 ttcttggcaa acaataatta aaaataaaac ttttatatga ttaaactcta aagaaaaagt
    14041 ttataaagaa gataatataa ttaagcagaa aataatgaaa aaaatttatt aaagtagaca
    14101 acgtaaggag aattttatga aagaaaaaat ttattacaat ggaaatatca tcacaatgga
    14161 agattcaata tgtggagatg ctattcttat aaaagataaa ataataaaaa agataggaac
    14221 aaaagaagaa gtatttgctt tgaaaaataa agatacagaa attatagatt tacaaggtaa
    14281 gacattgatg ccatctttta tagattcaca tagtcacttg atagcttttg ctactactct
    14341 aaagctagtt cctttagaag atgcaaccag ttttaaagac atagtaaaaa aaatacagga
    14401 tttcaaagag tcgaataata taaaaaaagg agactggata attggatttt catatgataa
    14461 taatttttta gaagaaaata agcatccaga taagagtgta ctcgatagtg catcttcaga
    14521 aaatccaata ctaatttcac atgcttctgg tcatatggga gctgcaaata cattggggtt
    14581 aaaacagctt ggtgttacca atgaaacacg tgacccagaa ggtggacata taggtagagt
    14641 agaaggcagt gaagaaccaa atggatattt agaagaaaat gcatttttta atgtagcatc
    14701 taaaataaaa caaccatcat caaatgagat ttttaattca attgaaaaag cacagaacat
    14761 atatctaagc tatggtatta caacagctca agaaggttta atggaagaaa atcaatttaa
    14821 cattttaaaa gcaatggcaa atcaaaataa attaaaaatg gatgttgtag gatatgtaaa
    14881 tttgaaaaaa agtaagtctg tattagataa taatagggag tttataaaaa aatatataaa
    14941 tagatttaaa gtaggaggtt ataagatttt cttagatggt tctccacaag gtaagacagc
    15001 atggttgagt agaccatatg aaaattcaga tgatggttat tgtggatatc ctatatacaa
    15061 agatgaagaa gtagaaaagt ttatagatat ttcactaaaa gaaaagatgc aactcttaac
    15121 tcattgtaat ggagatgcag cagcagacca gttgatagat gcgtttgaga aagttttaaa
    15181 cttaaaagag caaagtagtg aaaataatat aagaccagtt atgatacatg ctcaaacagt
    15241 aagagcagac cagattgatg atatgaaagt aataaatatg ataccttcat actttgtagc
    15301 tcacacatat tattggggtg atattcatat caaaaacctt ggagaagata gagcgtttaa
    15361 gattagccca cttaaaacaa ctatagaaaa gggattaatt tacactcttc atcaggatac
    15421 accagtaata gcacctaata tgctggaaac agtatggtgt gctgtaaata gaattacaaa
    15481 aaaaggtata caaataggag agaatgaaaa gataagccca ttagatgctt taaagggtgt
    15541 tactataaat gcagcatatc aatattttga agaagataaa aaaggttcaa ttaaagaagg
    15601 taaattagct aatttgataa tattagatga aaacccactt acaatcgacc ctatgaaaat
    15661 aaaagatatt aaagtattgc agactataag agaaggagaa gtattgtatt ctttgaagta
    15721 gatgaaagtt agatatttta taatctataa aaaatagttt tatataataa gttaggtaga
    15781 attaaataag attacagtta aatcaacaat tatatttttt attaaatttt aattattgta
    15841 ttgaag
//
LOCUS       DAEPOG010000090        15788 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEPOG010000090 DAEPOG010000000
VERSION     DAEPOG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.090"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            212..1429
                     /locus_tag="KR432_002273"
     CDS             212..1429
                     /locus_tag="KR432_002273"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBH0640803.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1524..1904
                     /locus_tag="KR432_002274"
     CDS             1524..1904
                     /locus_tag="KR432_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0640804.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2188..2670
                     /locus_tag="KR432_002275"
     CDS             2188..2670
                     /locus_tag="KR432_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0640805.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2691..3506
                     /locus_tag="KR432_002276"
     CDS             2691..3506
                     /locus_tag="KR432_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640806.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3557..5107
                     /locus_tag="KR432_002277"
     CDS             3557..5107
                     /locus_tag="KR432_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640807.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5204..6556
                     /locus_tag="KR432_002278"
     CDS             5204..6556
                     /locus_tag="KR432_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0640808.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6687..7445
                     /locus_tag="KR432_002279"
     CDS             6687..7445
                     /locus_tag="KR432_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640809.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7492..8211
                     /locus_tag="KR432_002280"
     CDS             7492..8211
                     /locus_tag="KR432_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0640810.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8335..9735)
                     /locus_tag="KR432_002281"
     CDS             complement(8335..9735)
                     /locus_tag="KR432_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBH0640811.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9739..10647)
                     /locus_tag="KR432_002282"
     CDS             complement(9739..10647)
                     /locus_tag="KR432_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0640812.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10908..11555
                     /locus_tag="KR432_002283"
     CDS             10908..11555
                     /locus_tag="KR432_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0640813.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11880..14519
                     /locus_tag="KR432_002284"
     CDS             11880..14519
                     /locus_tag="KR432_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0640814.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14577..15680)
                     /locus_tag="KR432_002285"
     CDS             complement(14577..15680)
                     /locus_tag="KR432_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBH0640815.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5950 a         1897 c         2751 g         5190 t
ORIGIN      
        1 agaatgtaaa aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata
       61 tgtttattgt atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa
      121 aaatcgttta catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa
      181 acaaaaattg gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtgaa
      241 agaggctaga gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa
      301 acttagcgaa aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg
      361 ttcttttaaa ttaagaggag cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc
      421 aaatggagtt atagcttcaa gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa
      481 aatgactggt ataaaatcta caatagtaat gccagcaact gcgcctttag caaaagttag
      541 tgcaactaaa ggttatggag cagaagtagt tttaaatggt gcggtttatg atgatgcata
      601 tgcaaaagct gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga
      661 taagtatgtt atagcaggac aaggaactat atctcttgaa attttcgaac aattagacaa
      721 taaagttgat actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt
      781 tgcagcaaaa gcattaaatc ctaatgtaaa aatagtcggt gttcaaactg caaatatacc
      841 ttcaatgaaa gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat
      901 agctgatggt atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt
      961 ggttgatgaa atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat
     1021 ggaaagtcaa aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag
     1081 tggtaagtat gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat
     1141 agacataaat acgttatata gaataatagg tgttgcttta gctaaagaag gaagaagata
     1201 ttcatttagt acaattatgg aagataagcc aggaaacttt gctgagttaa ctagaataat
     1261 aagtgagaat ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc
     1321 tctaggaaaa caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag
     1381 aataaagaaa gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta
     1441 tataatatac aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat
     1501 taacgaatta ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag
     1561 cagctttagg accatattct caagctataa aagctggaaa cttattattt gtatctggac
     1621 aagttcctct tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg
     1681 ctcaatcttt aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg
     1741 tagttaagac tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt
     1801 atgctgaata ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac
     1861 caaaagacgt taaagttgaa atagaagtaa tagctgtatt ataaaaatac aatattttta
     1921 atttgaaaag gctgaaaaac tatattctag tgattcagtc tttttataaa aaaataaatc
     1981 acaataaaga acatgataaa tattgtcgaa ttaaaaaata aaattaaaat aaatattgca
     2041 atatttttga gaaggtttta taatgagtat ataatttgaa acgtgtaact attaaattag
     2101 gcatgagttc ttggatattt ttatcctgag gattcatgcc ttttttgtat tgaaattcaa
     2161 aaatctattt aataaggagg aagtattgtg ttaaattttt tcaagaaaaa taaaagctat
     2221 aagttacatg cagtagtgag tggtaacagc attaatattg agaaagtaaa cgattctgtg
     2281 ttttcaaaaa agttaatggg agatggagtt gcaattattc ctaatagtaa tgttgttgta
     2341 gccccttgta atggtaaagt aactgtgctt acagaatcaa agcatgcctt tggtatggtc
     2401 tcagatgagg gtgttgaaat attagtccac ataggaattg atacagttaa tttacagggt
     2461 gaggggttta aaaatgaagt gtctcaagga gatactgtta aaaaaggtag tccaataata
     2521 agttttgaaa gagaaaaaat aaattctcag ggaattgatt gtacaacaat aataattgta
     2581 ttaaatcata gcgaattttc agaaataaat tgcatggtag aaaatgaagt tgttgcaggg
     2641 caagatactg ttattgaaat tatgaaataa taggaaggga ttaattcaat atgagagtag
     2701 taaaaatctt taataataat gcattatcta cagtaactaa tgataggaaa gaagctattt
     2761 tactaggtct tggtataggt tttaataaac gtccaggaga taaagttaat gaagataaaa
     2821 tagaaaagat ttattatgtt caagaccaca tgcaaacaaa atttttagaa ttattaaaaa
     2881 atgttagtcc agaagtgatg gatgcttcac aacaaattat aagtcttata ccaggaaatg
     2941 agggaaaatt taataataaa gggatattat ctttagtgga acatattagt tttgcaatag
     3001 atagaatgaa aaataatgtt tttttaccta atttaatgct tttggatata aagatgatgt
     3061 attcaaaaga gtttgaatta ggagtaaaag ctttagaaat tatatataga gtatgtcata
     3121 tccatttgcc tgaagatgaa gcaggttaca tagctttaca ttttgtcaat ttacaatcaa
     3181 atgataattt ggcatatgat actttaaaat ttgtaaaagg tagtattgat ttgattaaag
     3241 aatgttatgg attagaatta gacgaatcga gtttaagtac attacgtttt agaactcatc
     3301 ttaaatttct tgctcaaaga atttttaaga atgaaatttg tcaggatgat aaaatgattg
     3361 aaatgtatga ttatttaatt aacaatcatc ctaaaaataa agaatattta gaaaagttaa
     3421 atgtatatat tgaaaaggag tttaggtata aattagataa acctgaaaaa atttatctac
     3481 tactacacct aacaaaaatc ttataagaat gagatgaatt agtaatagaa atatataatt
     3541 ttaggggggg ataagattga aagaaaaact acttggagga ctggaaacat ttgcaaaggc
     3601 aatggttcag ccattaatgt atttatcgag tgcaggaata ttaatggtgg taggtgtact
     3661 acttacaaat gatatgttat taggagtact tccatttttt aaatgggagc caattcaaat
     3721 atttggaagc ctaatttata actgtataat ggctattatt aataatcttg ctgttatatt
     3781 tgctataggt attcctgctg cactagcaaa aaaagagaag cacaaagcag cgttaatagg
     3841 atttatgagt tatataatat accttacagc ttccaacact atgttaacat cactaggtca
     3901 actagcaaaa gaaagcaaga tgcttggact gatagggact ggacaagcta ctattttagg
     3961 tattcaagtt gtagatacaa gtgtatttgg tggaattatt ttagggtgta tagctggata
     4021 tgtatttaat cgtacaagca acaaaacatt taaaggagct ttacagatat actcgggagc
     4081 aaattttagt tttatatgca tgttctttgt atccatttta attggagttg tgagtaactt
     4141 tatatggcca ttagctcaac aaggaatagc ttctcttgca aatgtcatca aagatacagg
     4201 tacatttggt ttattcttat atggtttttt agagagaata ttagtaccaa ctggacttca
     4261 tcatttaatc tatacaccat ttcagttttc tgatttagga ggagtgctta aagttggtgc
     4321 agatacagtt tcaggagcct atgctatagt tatgacagaa atgaatttac cagttgagcg
     4381 tttctcagat agtatatact acatggcaac tggatttaca aagatgtttg gttatatagg
     4441 tataggagct gcctttattt atactgcata taagcaaaat aagaagaaag ttaaggcaac
     4501 gattattcca ctaataatta cggcagtatt agcctctgta acagaaccat tagactttat
     4561 gtttgtattc tgtgcaccac ttttattctt tgtacatgca gttatagctg gtctttttat
     4621 agctttatta aaaatatttg atgttactgc attatgtgga gggaacttat tagtatcaat
     4681 tataatgaat actacagtag gtgtagaaaa aacacactgg ccagtaatga tgatattagg
     4741 acttgcacaa attattgtat attttgtagt atttagtttt ttaattaaga aatttaatta
     4801 taaaactcca ggtcgtgaag atgagagtac agctacaggc gaagaacaat caaaacaact
     4861 taatttagat gcaggtatag aaaatataat tgatgggtta ggcggaaaag aaaatattaa
     4921 tacagtagaa aattgcatca ctagattacg tgttaatgta aaagatgaaa gtaagattaa
     4981 tgaagatata attaatttaa cgccaaatag cgggattgta aggaaaggaa aagatataca
     5041 aataattttt ggacttcatg tgcatgaggt aagacgtgca gtagaagatt ttttagaagc
     5101 ttattaaaga tatatattta tatagcaagg gtaaataaca gctttaagtg ctgtttacta
     5161 tttattaaag atacatataa tttatagagg aggaataatt attatgatta ttactattgt
     5221 tggtggagga agcacattta ctccaggtat tgttaagtca attgcgttaa gagaaaaaga
     5281 gttagagctt gaggaaattc gcttatttga cattgataaa gaacgccaag ataaagtagc
     5341 tgtagttgtc aactggatac taaaagaaga tgttaaaaca gatgttaaac taactgtaac
     5401 taattgtgaa aaagaagcgt atacagatgc tacatttata tttgcacaaa tgcgtgttgg
     5461 taaatatgaa atgcgtgagc aggatgaaaa aattccattg aaatatggtt gtgtaggtca
     5521 agaaacttgt ggttgtggag ggatggcata cggactgcgt acaatacaac caatgataaa
     5581 ggttattgat gatgtagaga aatatgctaa aaaagattat tggatattaa attactctaa
     5641 tcctgctgca atcgtttctg aagcttgtcg taagttaaga ccaaatgcaa ggattataaa
     5701 tatctgtgat atgccaatag ctattataga tatgatagcg gagtcattaa atatcaatga
     5761 cgttcataat atacgctatg attattttgg tctaaatcat tttggctggt ttacttcaat
     5821 agattataaa catagagact taatggaaga gattaaggaa tacataaaag aaaataaaat
     5881 attattacca aaatcttata tagaaaactt aaataaaagt cgtagtacag gaaatcgcca
     5941 tgctgtaagt agctggatac atgtttggga atctgtttat acaatgttag aaagcttccc
     6001 aaaatatctg ccaaacactt atttaacata ctatctttta agtaaagaaa cagtggaaca
     6061 ttcaaacata aatcgtactc gtgcaaatga ggttatggaa agccgtgaaa aagacttatt
     6121 tgaaggtgtg agacattact tagaaactgg agaaatttct gaaaaagtat tttatgcagg
     6181 ttcacatgga gattggatta gtgatttggc agtttcaatt aaaaatgata cacgtagtcg
     6241 ttttttagtt ataactgaaa atcgtggagc tataccaaac atgccatatg atgcaatggt
     6301 agaaattcca gcatatattg gtaaagatgg tccagaagtt attgctcgag gtccaatacc
     6361 tctattccaa caaggattaa tgatgcaaca acttaacagt gaaaaattga tagtagaagc
     6421 ttatatagaa aatagttatg aaaaagcttt acaagcattc acattaaata aaactattcc
     6481 aagtatgaat gttgctaaag aaattttgga tgaaatgata gaagttaata aggaatactg
     6541 gccagaatta agatagtagg ataaaagaaa gggtttttat ttaataatta gatatgtttt
     6601 cagagtcttt tatcaaaatg tattcacaaa catatggcaa tatattaaaa taaagagtga
     6661 tatttttaga agaatggggt gatgcaatga acattataaa taaaattttt tggcaagaag
     6721 ataggattgg catgattaca aataatttag aggcagatac acatagccac tgcatgttac
     6781 aactgttctt agggatagaa gacagcattg aaataacagt caatgaaaaa ttagttaaat
     6841 gtaattgtat catagtagat aaaaatatct ctcattcatt ttcagcaaga aaaaaagttt
     6901 attactctgc tattattgaa cctacatcta tatatgcaga acagcttacc tcaaaaatga
     6961 atgattttgg ctactggata tgtgacaatg atggattaga gaaattacgt caacaaggaa
     7021 cttttttgat atataactct agtaaagaac aatatttaag atttatggaa atgcttaata
     7081 actatttgaa tatacctata actctcaaac attatgatga cagaattact gaattgctca
     7141 atttattacg tacttgtaat tgtgataatc atactatttc aagttttgca gataaagtgt
     7201 ccctatcagc aagtagatta tcacatctgt ttaaagaaca aatagggata cctttaaaaa
     7261 gctatatctt atttcatcaa atggagtgtg cttttagaga attactttct gggaaaaatg
     7321 ttaccgaagc ttctatgact gctggattcg atactccatc acactttgca gggacagtta
     7381 agcgaatgat gggaatgcct gtttctttat ctttgaaaga tagcgaattt ttgaaagtat
     7441 attaaatatt ctcgttatat aatgaagttg tactaagaaa ggggctttgt tatgaataaa
     7501 gaatatttag aggaaacaaa aatgttaaat tataatgaac cacaacttaa gcttttggta
     7561 tcatcaaaga attggttgga attagatgat tttcataaaa taaaatctat ttatgagttt
     7621 gtacagaatg atattttatt tggatataat gcttctgata tgttaagcgc aacacaggta
     7681 ttaaatgatg gtatgggaca atgcaataca aaagctactc ttttaatggc attattacgt
     7741 gcagtgaata tcccttgtag gcttcatgct tttgatgtaa caaaagattt tcaacgaggt
     7801 gctacatcca aattgatttc actattagca ccaaaatata ttcttcatac ttgggtagag
     7861 gtattttatc aagataggtg gattgctctg gagggtgtca taactgataa aaaatattta
     7921 gaagcaattc agaagaagtt ttttaatcat ggtggaacgt ttaaaaagta tgcaatagct
     7981 acaaatgatt tgaaaaatac atctattgat tgggatggta aagatacttt tatccaaaaa
     8041 gaagctatcg tgtatgatta tggtattttt ccatcaccag atgtattttt ttctactcat
     8101 tcacaacata tgtcaaaatt aaaaaatttc atatatgtac atcttatcag aaaaataatg
     8161 acaaaaaatg tatgtaaggc tagaaacaat tatattgata aaaacgaatg agtgtttaag
     8221 aggtaactat ataggaaata tatagaataa ttaaaactgt actggttaaa atgaataaaa
     8281 aggagttatc atagagcaaa cttaaattgt tcataggata actccttttc tgtattaact
     8341 tctaataatt tgatgtcttt ttataaaaca tatagataca attaaaccag taaatgcaag
     8401 agtagctgct attgaaaatg caacatctat tccatgaatg ttagacagca tgtttgaact
     8461 catatgagct ttcttagtag cacttgtcat tatagtaata agtatagcag aacctatagc
     8521 tccagatatc tgacggagtg tgttattaat tgctgtggca tgagatataa gttctctatc
     8581 taatgtttta attccccatg tagttaatgg cattaaaacc attgaaatac caatcatacg
     8641 aatacaatac atcaatgaca catgaattaa agatgtatct ctgcctaaaa aagcgaagga
     8701 taaagttcct aaaaacaaac atcctgttcc taatattgat agagcatgag gtccatattt
     8761 atctaaatga cgtcctgcaa ttggattgag tacaaccatc aatatagcac caggtaacat
     8821 taatgaacct gaagataatg cagaaaatcc tcttacagat tgaacgtaca gaggaatcat
     8881 taatgtagca gacatcattg aggcatatac aatcataatt aatattgtag aaataagaaa
     8941 tggtttattt ttaaaaaccc tcaattctaa aaaaggttct ttagatttta attgacgcaa
     9001 tgtaaataaa attaatgaca taattcctat taatattggt aaatatgtgg caatattagt
     9061 ccacccataa ttaccttgat tagtaaatcc aattaataaa ccaccaaaac ctagagatga
     9121 tagtactatt gaaggtatat ctaactttag tttttgagtt tccccaacat ttcttaatag
     9181 aataaatgat agtataacat ctaatatagc aataggggaa agaaacagaa ataaactatg
     9241 ccaaccaaat gaatctacta accatcctga taaagttgga cctattgctg gagcaaatcc
     9301 tactgtaata ccaactaaac tcatagctgc accacgtttt tcctcagggc ataaatagag
     9361 tataataact tgtaaaagtg gcattagaga accagaaccc attgcctgca acacacgagc
     9421 aatcaacata gttgaaaaat tattagaaaa aagtgcaata atacatccta tagaaaagat
     9481 agacattgat gtaataaata actttctagt actaaatcga tttattaaat aagctgttgt
     9541 gggtacaata atacctgcaa taagtagata aattgttgtt aaccactgac caatatcagc
     9601 agtaatttta aaatctgaca ttatattagg taatgctgtt gacaatagtg tttgactgag
     9661 agaacttata aaagcaccag tcattacaat tgctattatt aaatttcctt ttttattgtt
     9721 actttgggtt tccataattt ataacgtctc cttttttaat tgatttttaa tgctgttcaa
     9781 taaaagtact tttggtctta aagtgtcttc tttttctaaa tatgcaatca ttgcatccat
     9841 aattaaagaa tcatattgtt gattttttcc ctcgacgtat aattttttta ttgcttctag
     9901 taatttagga ttttcaagga gagaagtatc agttttgatt agttcttcta tatcttgtat
     9961 cgttttaaaa aatagcgatt tattatgaag ggaaaagtaa acttcatctt cttgtttatg
    10021 taaaaacatt gatttttcaa ttaaaggatt tagattatcc atattttcta caactaaatc
    10081 tagaatttgt atagtaaatt tggcaataaa gtatacattt atttctcttt tttgtacatg
    10141 aataatgtat ataaactgat gaattagagc atccaataag aaaactaaat caaatataat
    10201 tttttctatt ttatcaccat agttatcaaa taagcttaat ttgtattcat tcaataagtt
    10261 agctcgtgat tttgtttgca atttacttgt agccttacgt tgctcttcag aaaaattttc
    10321 tcttatatag gtatttatgg tagttctaga aaaaatattt ttccatacag caattatctt
    10381 tctgtttaac ttttctgatg atggaatgtc attattttcg tttatggaat gtataatttc
    10441 taaaaacttt tcatcataat aattgattat ttcgcagata aaactttcct tagtttcaaa
    10501 atgcttgtaa aaagtagctt tagacatttt acactctttt acaacatccg atattgaagt
    10561 attttgaaaa cctttttggt caaaaatttt ttgtgcaata tttataatga gcctttgttt
    10621 ttctgtcact ttattatctc ctttcacgca aaaacgttta gtactatata gtacctacaa
    10681 gttttaataa atatattata catcatttgg aaaaaaaatc aatatataga ctgtaatatg
    10741 aaatttttga aaaatttgtt gttattttta gtcgtataaa aaagaaactt aagagtttct
    10801 taagaattgt ttaaagaagt tgcaataaat tagtggtatg attatagtat gaaagagaat
    10861 gtcttgaaat ttttctgtat caaattccaa ttatggaggt gtagtatatg aaaaagaaaa
    10921 tttgtgtagc aataattttt gctctattta tagtttttgt agaaatgttt attgatagct
    10981 ctagcgaagg aagtaaaatt atgacagctg aattaagtaa atacaggaaa ggcgtaaatg
    11041 tattatcaat aaacaatatt atagtagcga ataaaaaata tagtttgccc aaagattatt
    11101 ctcctcagga aagcagtgaa gcgagagatg ctttttataa aatgaataaa gatgctcaaa
    11161 agtcaggatt aaatttaaaa gcttttagta catatagaag ttatgaatat caagatagat
    11221 tatttaaatc atatgtaaaa gaacatggag aaaaagaagc aaatagattt tcagctaaac
    11281 ctggagagag tgagcatcaa actggacttg cttttgatat tggaggagat gaccagtctt
    11341 gttgggcaaa taaaaagttc aacaatacaa aagaagctaa gtggttgtat gaaaatgcat
    11401 ataaatatgg ttttatactt agatatccag aaggtaaaga gaatataaca ggatatatgt
    11461 atgaatcttg gcactataga tatgttggaa ctgaacatag caaaaacttt gcaatgaata
    11521 atttgacttt agaagaatat ttacatataa attaagagat ggtttgttaa aattaggtta
    11581 aaataaaaaa taacctagac tattagctgt gtaaagtgat ataatgtaaa tacaatttaa
    11641 gataactact cgaaggggag tagcttacac gaaagtggta attaatcgtc aagacagtag
    11701 aaatactcgg ttaattagca agggattcct tgatagcaag accttcaaaa taagcattag
    11761 gtatgtttgt tttggggtct ttttttattc aaggaatttt tatttaaaaa aatttaaatt
    11821 tcaagtatac ataagagccc ccaaacataa aataaattta ttgaagagga gggcaacaaa
    11881 tgtcttacaa aagtacttat caagaagtaa ttaaaaattt agactcaagt ttatctggtt
    11941 tgagtaaaga aagagcagaa gagttgttgg aaaaaaatgg agctaatgaa ttaaaagaag
    12001 cagataaggt tcctacttat aagcttttcc tagaaagttt taaagaccca ttagtaataa
    12061 ttctattgat agctgcttta gtacaaatat ttttaggtga aacagtggag tctataataa
    12121 tttttgcagt tattattata aactctgttc tgggtgttgt acaaacaaaa aaagcagaaa
    12181 gttcattaga aagtttgaaa aatttatctg caccaaacgc aaaggtaata agagacaata
    12241 aaaaaatgac aattccagca aaaaatctag tagttggaga tattgtattc ttagaagctg
    12301 gcgattacat accagcagat ggaaggctta tagaagcaca atctctcaag gttgtagaag
    12361 ggatgttaac tggagaatca gaaccagttt taaagcatac agaaaaaata agtgaagatg
    12421 taagtttagg tgaccagaaa aatatggtat ttagtggtgc tgtagttgtt tatggtagag
    12481 gttcttttgt agtgactgga acaggtatgg atactgagat gggtaaaata gctgggcttt
    12541 tagaaagtgc tgaaaataaa cttacaccat tgcaacaaaa aatagaggat tttagtaaaa
    12601 aacttggtgt aggtattgta atacttgctc ttttaatatt tataattcaa gtagcaagaa
    12661 attactttat ggctggaaat ggagaaatga cacaaattat tttagattca ttcatgttct
    12721 ctgtagcagt agcagtagca gctataccag aagctctatc atcaatagtc actatagtat
    12781 tggctgttgg aacaaatagt atggcaaaaa aacaagccat aataagaaaa ttaccagcag
    12841 tagaaacttt aggttcaaca agtgtaatat gtactgataa aacaggtact cttacacaaa
    12901 ataaaatgac agtagttgat tattatatgt atggaactaa taatgatgag ttagataaag
    12961 aaaaagtaaa ctattcttat catgctaaat tattgactat ggtttctaca ttgtgtaatg
    13021 actctcatat aactagtgat ggtaaagaag ttggtgaccc aacagaagtg gctttaataa
    13081 attattcaag taaaaatgat ttagattatg ataaattaag aagtaagtat atcagaataa
    13141 atgaaatacc atttgattca gacagaaagc ttatgtctac tgtaaatagt atatatggag
    13201 attatataat gtttacaaaa ggtgctccag atattgtgtt tagtagatgt aaatatgctt
    13261 tgaaaaatgg ttcaaaagta gatataacag atgagataat agaagaatat aaacataaaa
    13321 atgaagagtt ttcaaataga gcacttagag tattagcttt tgcaataaaa gatgtcccag
    13381 aagaagattt tataccttca attgatgacg aacatgacat gacattggtt ggaataatgg
    13441 caatgataga cccaccaaga gaagaagtaa tggatgcagt aaaagaggca aagagtgctg
    13501 gtataaaaac tgttatgata actggtgacc ataaaacaac agcagcagca attgcaaaag
    13561 agattggtat aatggaagaa ggagacttgg ctcttactgg aaaagagctt gatgctttga
    13621 gtgaagaaca actatatgaa aaactagaaa atataagtgt atatgccaga gtctcgccag
    13681 aaaataaaat tcgtatagta aaagcatggc aacataagaa taatataaca gcaatgactg
    13741 gtgatggtgt taatgatgca cctgcattaa aacaagcaga tataggaatt ggtatgggaa
    13801 gtggtactga tgtagcaaaa gatgcttctg ctatggtatt ggttgatgat aactttgcaa
    13861 gtatagtaaa tgctgttgaa gttggtagaa cagtatacag taatataata aaatcaatta
    13921 cttatctatt tgcaggaaac ttaggagcta tagtatccat agtatttgca gtgtttgcag
    13981 gttggtcaaa tccatttaca gctttacaac ttttgtttat aaacttagta aatgactctt
    14041 taccagcaat tgcattaggg tttgaaaagt cagaaaaaaa tataatgaat aaacctccta
    14101 gacacccgga tgaaagtata ctagcaggag gaacattgaa ggcagttgct ataagaggtt
    14161 gtataatagg tgtagttaca ataattgcac aatatatagg attacaagta tctccagaac
    14221 ttggagtagc aatggcattt tcaacattga tattagctag aataatgcaa acattacctg
    14281 cacgttctaa taatcaaaca gtaataaaat taggtttttt cagcaataaa tacgttatag
    14341 gagcagtttt agtctgtctt gggctttact ctttaacact aataccagtg ctaagaggaa
    14401 tattctctat accaacaacg tttggattgt accagttggg gatttgccta gggttagcat
    14461 taatttcatc aatattaatg gaacttaaca aattaataaa tagaagtgaa gtaaaataat
    14521 caaaaaatta aagggcatat tgattttaac aatatgcctt ttataaatag tctaatttat
    14581 tctatattat cgattatttg gaatgctttt ataacttcag gtaattcttt ttcttcaggc
    14641 attctaaatc ttatgaaatt tttatcaagt ccagtaaaat cacttccaga tataactctt
    14701 atttttaatt tagcaaactc ttgttgtaaa tctatgtcct tgtttttatg ttcaactgtc
    14761 ataattgata cagtatcact agtttcagct atattaaggt ttttccatgg aattagtaat
    14821 tgatttttta tatcttttgt tttttctttt aattcttcta taaattgaac atcatctaat
    14881 aaatttgctg caatactacg agatatttca gacacttcgt aaggagtaga aatcttttta
    14941 atagggctaa caagttgttc tggaagaact gcatatcctg ctcttagtcc tgctaaacca
    15001 aatccttttg aaaatgtttt taaagcaatt acattatcat aattatttag aagcttgaca
    15061 gctgaatttt cttttggcat gtattcacca taagcttcat ctaccataac tgctatatca
    15121 tatttagctg cttcctttac tatattttca attgatgata aaggtataat ttgtcctgta
    15181 ggattgtttg gattgtctat gtaaattact ttatattctg gatttatttt ttcaataaat
    15241 tctttttcat taaacttgaa attatcctct ttctttaaaa gaacataatc ataagttgca
    15301 ccatgcattt ttatatctgt ctcatattct gagaactgag gtgaatatcc taaaacttta
    15361 tctccctttt caataaatag tctatttaaa aggtaaataa catgtataga accatctgcc
    15421 aaacaaattc tgtcagtatc taatgctata aaatctttcc aataatcgac tatattatct
    15481 tttaaagcaa ttgaatgagg atatcctctt atcatatcaa attttaaagc attaaaagct
    15541 tctacagctt tgcttgagta agacacggta tttatacctt caccacagtc tatctcgaat
    15601 acatcttctg aatgtccaga ttctattgca taactttttt tcaactgcat ttcaatagct
    15661 ttgttaattt ttagtttcat actttatcac tccttattta gtcaataata aatgttataa
    15721 aagttgctag atttaaaata ttatgaaaat atataatagt aaaaaatgaa tataaatatt
    15781 ataatagc
//
LOCUS       DAEPOG010000091        15768 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEPOG010000091 DAEPOG010000000
VERSION     DAEPOG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.091"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            269..712
                     /locus_tag="KR432_002286"
     CDS             269..712
                     /locus_tag="KR432_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0640816.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            743..2560
                     /gene="ftsH"
                     /locus_tag="KR432_002287"
     CDS             743..2560
                     /gene="ftsH"
                     /locus_tag="KR432_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0640817.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            2738..5440
                     /locus_tag="KR432_002288"
     CDS             2738..5440
                     /locus_tag="KR432_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBH0640818.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            5474..6172
                     /locus_tag="KR432_002289"
     CDS             5474..6172
                     /locus_tag="KR432_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640819.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            6511..7467
                     /locus_tag="KR432_002290"
     CDS             6511..7467
                     /locus_tag="KR432_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBH0640820.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            7644..7799
                     /locus_tag="KR432_002291"
     CDS             7644..7799
                     /locus_tag="KR432_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640821.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            7929..8942
                     /gene="aroF"
                     /locus_tag="KR432_002292"
     CDS             7929..8942
                     /gene="aroF"
                     /locus_tag="KR432_002292"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0640822.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            8964..10028
                     /locus_tag="KR432_002293"
     CDS             8964..10028
                     /locus_tag="KR432_002293"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH0640823.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            10028..11341
                     /gene="aroA"
                     /locus_tag="KR432_002294"
     CDS             10028..11341
                     /gene="aroA"
                     /locus_tag="KR432_002294"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH0640824.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            11319..12398
                     /gene="aroC"
                     /locus_tag="KR432_002295"
     CDS             11319..12398
                     /gene="aroC"
                     /locus_tag="KR432_002295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH0640825.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            12401..13597
                     /gene="pheA"
                     /locus_tag="KR432_002296"
     CDS             12401..13597
                     /gene="pheA"
                     /locus_tag="KR432_002296"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBH0640826.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            13644..14453
                     /gene="aroE"
                     /locus_tag="KR432_002297"
     CDS             13644..14453
                     /gene="aroE"
                     /locus_tag="KR432_002297"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0640827.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            14465..14998
                     /locus_tag="KR432_002298"
     CDS             14465..14998
                     /locus_tag="KR432_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBH0640828.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            15019..>15768
                     /locus_tag="KR432_002299"
     CDS             15019..>15768
                     /locus_tag="KR432_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBH0640829.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNL"
BASE COUNT         6386 a         1635 c         2807 g         4940 t
ORIGIN      
        1 attttaactt ttaaaagaga ttaaaaaggg agaaatcagt gtaaaatata tatatcgatt
       61 ttttattaaa ttttattaaa aatatcctgt ataaaattaa atatcaataa gataacaaaa
      121 ttccaattga agttacaata ttgtaattga caataaatac ttaatactat aatatttaat
      181 acattaaaca ttatagtatt aagtatttat ttacagtatg cacttcagcc taaatttgaa
      241 ataaagtatt ttcgtaggga gcgtttgtat ggatgagatg agttttaaga aagagctact
      301 agagttgact agagatataa atatgaaatt tacaacttta ttaagtgatt tttatcagcc
      361 acttggtatt acagcagtgc aagctttgat tttatctgag ttatgtgaac atggagagaa
      421 aaaaataagc gatttaggga agaacttaaa catgacaaat agtaatgtat cagtaatctg
      481 tcaaaggctt gaaaaaaatg gctttttaaa tcgcataaga gatatagaag accaacgaat
      541 tgtaaaagta aaagttacaa ataaatcatt ggatatacaa gaacatattt caagtagtat
      601 ttttgatagt tattttgaaa atatgaccag tgaaaaattg caagatatgg aagatataat
      661 agaaggttta gagaaattaa ataaactttt aacttacatt gattgcaaat aacataaaaa
      721 taatttaaag gagaataaaa ttatggatga acataaaccc caaaaaaggt cagcactcta
      781 ttattataca gttataataa tagtagtaat gtttttaaat gctttaatat ttccaagttt
      841 aatggataaa aaaatggttg agattgacta tggaaagttt ctcacaatgt tggatacagg
      901 aaaggttaaa tctgttgaga tagaaaataa tagaatagca ataaatccat ctagtagcaa
      961 ggataagaat atatacataa ctggtagaat ggatgaccca gaattggtag atagactaaa
     1021 aaatgcaaaa gtagaattta ctaaggttat tcctaaggaa aattcacctc ttttaagtat
     1081 tttacttaca tggataatac ctataggtat tcttatgcta tttggtaact taatgatgaa
     1141 gtctatgcaa aagcgaatgg gtggtaatgc tatgcaattt ggaaaaagca acgccaaaat
     1201 gtatgtatct gctcaaagtg gaaaaagatt taatgatgta gctggtcaag atgaggcaaa
     1261 agaagcttta actgaaatag tagatttctt acacaatcca gaaaagtata aaaagatagg
     1321 agcacaaatg cctaaaggag cacttttagt aggacctcca ggtacaggaa aaacactcct
     1381 tgcaaaagct gtagcaggtg aagcaaatgt accatttttc tcaatatctg gttctgagtt
     1441 tgttgagatg tttgtaggaa tgggagcttc taggattcgt gatttattta aacaagcaaa
     1501 agaaaaagct ccatgtatag tgtttataga tgagattgat actattggta aaaagcgtga
     1561 taatggaagt ggattaggtg gaaatgatga acgtgaacaa actttaaatc agttgttaag
     1621 tgagatggat ggttttgatg gtggaattgg agtagtaata cttgctgcta caaatagacc
     1681 tgacagtctt gacaaagcat tacttcgtcc aggaagattt gatagacgta taccagttga
     1741 acttccagac cttggaggac gtgaagctat attaaaggta catgctcaaa atgtaaatgt
     1801 aagctcagat atagattata atgcaattgc aaggtctaca tcaggagcat caggtgcaga
     1861 acttgcaaat attgtaaacg aagcagcact tttagcagtt aagtctggca gagatatagt
     1921 agaacaacag gattttgatg aatctgttga aactgtaatt gctgggtatc aacgtaaagg
     1981 agctgtaatt tcagataagg aaaagaaaat tatagcatat catgaaatag gtcatgcact
     2041 tgttgctgct atgcaaaaac attctgcacc tgtccataag attactataa tacctagaac
     2101 ttcaggagca ttgggatata ctatgcaagt tgctgaagat gagagtgtac ttatgtctaa
     2161 agaagaagca attgataaaa taactacttt tacaggtggt cgtgctgctg aagaggttat
     2221 ctttaacact tgtacatcag gagcatcaaa tgatatagaa caagctacta aaattgcaag
     2281 agctatggtt actcgccttg gtatgagtga agagtttgat atggtcgctc ttgaaactgt
     2341 aagcaatcaa tatttaggag gagatacttc tcttgcttgt tcttccgaca ctgctgctaa
     2401 aatagatgaa gaggtattta aaatagtaaa ggcttgtcac aaaaatgcaa ttgaaatctt
     2461 agaaaataat attgataaat tgcatgaact tgctaattat ttattagaaa aagaaactat
     2521 tacaggtgat gagtttatgg aaatacttaa cttaaactaa aatgattata cacaagataa
     2581 aaatgcctat tgttaggcat ttttatttta caactaaatt ttttaaattc atatttaaat
     2641 ctttacataa tcttaatcat taaaaataca tcttaactac aattttatat cggaattgat
     2701 attataatag taatgaatgg tgtaaggagg ttttttaatg gatagaccta atcctgatat
     2761 tctattagaa aaaattaaaa atgaagaaga aaaactaagt agaggtcaat taaaaatatt
     2821 ctttggttat gcagctggag taggaaaaac ctactctatg ttggaatctg cacaaaattt
     2881 aaaaaaagtg ggtgtagatg tagtagttgg atatattgaa ccacacacta gaccagaaac
     2941 attggctcta cttgatggct tggaaattct tcctgtaaag gagatagaat ataaatctat
     3001 aaaactaaaa gaatttgatt tagatgctgc tttagcaaga aaaccagaag ttatattagt
     3061 agatgaattt gcccattcta atgttgctgg gttaaggcat acaaaaagat ggcaagatat
     3121 agaagaattg ctgttagcag gcataaatgt ctacactact gtaaatgtac aacatttaga
     3181 aagtctaaat gacattgttg aggtaattac aaacgtatca gttaaagaga ctataccaga
     3241 taagtttttg gatacaaata cacagttaga acttatagat gttgaacctg atgtacttct
     3301 tgaaaggttc aatgaaggta agatatataa gaaagaacaa gctactagag caaaaaataa
     3361 tttttttata aaagataacc tggttgcact tagagaaatc gcacttagaa aaacagcaga
     3421 gagagttaat aaagaagttc aaatgaccag actatcaaag ggtgatgtaa ctgtaatacc
     3481 tacatcagat acacttcttg catgtataag tccatctcca tcttcagcaa aagttattag
     3541 aactgcatcc agaatatcag acagtacatt tgcaaaatgg atagctttat atgttgaaac
     3601 tccaaacaca gcaagacttc catttgatga acaaaaacaa ctacaaaata acctaaaatt
     3661 agctaagaaa ttaggtggag aaataattgt tttacatggt gaaaatataa tagagcaaat
     3721 acttagaatt gcaaaactga gaaatgtaac aaaaattgta ataggtagaa atcattcaag
     3781 taataaaaga tttagtaaaa gatttaaaaa agatatagtt gacaaattaa ttgatgaagt
     3841 tgattatata gatatacata taattccata taaatttgcc tcagatgtta aatacaaacc
     3901 taaaaaggat aaatcatcaa taaaatctaa atttaaaatt agcaaagttg attttataaa
     3961 acttatattt attactttga tgataagtat tttagcatat gcagtacaaa gcatgggatt
     4021 tataagagaa aatatattgt taatatatat gttgggtgta gttttagttt ctatgtggac
     4081 taaagggtat agtacaggta taataagttc tgtatttaat atagttcttt taaattattt
     4141 ttttacagca ccattatata cattatctat agcagattca aactatatag taacactggt
     4201 tgtattctca atagtaggta taataactag cactcttacg tctaagatac agcatgaagc
     4261 agaaactgct gctaaaagag aagaaaatac aaaaatgata tatcaaataa ttaagggatt
     4321 tttaagatta tcaagtaaag atgatatagt aaacaaaggt atagaacttt tgtctcttag
     4381 cttaagtaga gatgtaattt gttatttaaa tgtagataag cataataaat caaaacttta
     4441 caaaaaaaat actaataata aggataaaaa tgatttggat agtgaagatg aaaaagctgt
     4501 tgcaagttgg gttttatcca attcaacagt ggcaggaaat gacacggata cacttcctgg
     4561 ttcaaaggga tactatatac caattgtagg aatgaattta actttaggag tagtaggaat
     4621 atcatgtatt gattcaaaat tggatacaga agatataagc ttaattgaaa caataattgc
     4681 tcaaatggca atagcgcttg atagagaaat cttgtcagaa gctaaagaaa ataccaatct
     4741 tgagattgaa agggaaagat taagaagtaa tcttttaaga gcagtttcgc atgaccttag
     4801 aactccacta gcaggaatat ctggtgcagt aagtacaatt ataaaaaaca agggtactat
     4861 aggtcaagat ataatagatg aattacttag tggtgtatat gaagatactc agtggttaat
     4921 aagacttgtt gaaaatttac ttagtatgac caagatagat gaagggaaat tggaagttaa
     4981 aaaacattct gaactggttg aggaaattat atcagaagcg ttacaaaaaa taaaaaaaag
     5041 aattgaaaat gcacttatag atattaatat accagaacag atattatttg ttcctatgga
     5101 tgcaaaatta atagaacaag ttttaattaa tttaatagat aattctttga aatattcaaa
     5161 ggaagattgt aagatagaga taaatgtata cgagaaagat gattatgttt ggtttgaagt
     5221 atctgataat ggtcctggta tatcaaagga acttaaaaag catatctttg acagattttt
     5281 tactggagaa gaaggagcta aagattccag aaagggtgtt ggacttggat tatcaatttg
     5341 taaatctata attcaagctc ataaaggtga aattatggta gaaaacaata aagataaggg
     5401 ttcaactttt aagtttagtt tgcctaagga aaatgaatag ttttaaattt taaaattagt
     5461 tttggaggaa gaaatgagta aaaaacttat attgttagta gaggatgata agacaataag
     5521 aaaatttata tctactgcac ttttaacaca ggattatgat gtcaaggagg ctataactgg
     5581 aaaagaaggt atatctattg ctgtctcata ttctccagat gttgttcttt tagatttagg
     5641 cttagaagat atggatggaa tagaggtaat aaaagctatc agacaatttt caaatatacc
     5701 aataattgta gtttcagcta gagagcaaga tagagataag gtagaagtat ttgatgcagg
     5761 ggcagatgat tacttaacaa aaccattctc catagtagag ttattagcaa gagtaagagt
     5821 cgcatttaga cattcacaag tagaagctca acaaaaagat gatgttaaat ctacgtttga
     5881 agtagataaa ttattgattg attttgataa aagaaaagtt attgttgatg atgttgaagt
     5941 ccatttgaca cctattgaat ataatattct ttcattactt gcaaagcatc atggaaaagt
     6001 gcttactcat aattttataa taaaagaaat atggggtagt gttataggga atgagactaa
     6061 gtctttaagg gtctttatgg caacattaag acgaaaaata gaaaaacaac ctgcaaaccc
     6121 aagatatata attacagaag ttggtgtagg atacagatta aatgatgaat agttgtaata
     6181 tattaattat tagattatgt gcttattaat atatattaaa attatatagt ttagttaaaa
     6241 taagaatgaa gtgtatgtgc ttgttgtcag tttagcacat gcattttatt ttttagcttt
     6301 ttagtatcaa taatatgaaa tatgttttaa aataagtata tagtgtaatc tgtaataaaa
     6361 actttggtta aataataaat gtatttaaca cttaccttat aataaattta atgtaaatat
     6421 taaataataa ttgttaaaat agtgtaaaat aattacagta aggattataa aaaattttta
     6481 gaatgtaaat tgaaatttag gagtagatgt atgtatatac tgaatttaaa tacaagagag
     6541 actattgaag attttagaga taaattttat gtagcagaaa attcttatct tatattaagt
     6601 gctccaaaaa atttaaaatt gcttaaggaa actttggata tagatgaaat aacttttaat
     6661 gattgtctta aatttgatga aataaccaaa cttgatttat ttgataatta cgatttttta
     6721 agcctaaata catttgaact aagagatgga gaagctgtta tagaagaagt aaatatgtat
     6781 ttatcagata attttatatt ggttgtagtg aatgaagaac actttttatt tgaatttgta
     6841 aaaaatatta ttttgaagaa ctctcaactt gaaaaaaatc cagttattaa tctatttaag
     6901 ataaactact tgattcttag agaagttata aaaaatggtt ttgagtcttt ggaaaaagtt
     6961 gaagagttaa ttcttcaaat agaagatgaa atgatggaca acattaataa gaatcatgta
     7021 tctagaataa gtgatgtaag aggattaaca agaattatag ttaagaatac tagacctctt
     7081 ttgtatattg gggatagaat agttaaggaa aatataagat atttaaaata ttcaaatgta
     7141 aaaaaatata atttagaaaa ttttcaaggt atagattttg gaattgacaa attatacaat
     7201 ttcgcattgt caacacgaga gttagcagat aaattattag atatttattc ttccagagta
     7261 ggtgagaaaa caaataactt aattacaaaa ttaaccttgt taacagccat atcagcacca
     7321 cttacaatta taactggtat atatggaatg aactttagat atatgccaga attaaactgg
     7381 atttatggat atccagcaac tttatttttt atgctatgta taatttttat tggaattata
     7441 atatttaaaa taaaaaaatt attgtaaatt acagatgatg tttaattgat tttataaaaa
     7501 tacatcaatt gagaagctga tttaagtaaa taaaaaaata ttttaaattg aaaatattta
     7561 tagaaaatat tgacaattat attttctttg ttataattat taaacaagaa gaataataaa
     7621 ttataaaaat ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt
     7681 atagcttttt ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca
     7741 aaaatatatg tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa
     7801 ttttataaaa atgattattt tatatattta tataagattt agtgcttgat agattatttt
     7861 agagaactca ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg
     7921 tagtaaaaat gatagtagta ttaaaaatgg gagcagataa aaatgaagtt aaaaaattaa
     7981 ttgaggctat tgggagagaa ggagtagaag ttaatcccat agatggaaca gaattaactg
     8041 ttttaggttt agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataaga
     8101 tagttgaaaa agttatgcat gtagtagagc cttttaaaaa ggccaataga aaatttcatc
     8161 cagaaccttc aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga
     8221 tagctgggcc ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga
     8281 aaaaatcagg tgcaggattt ttgagaggtg gagcattcaa accaagaaca tcaccatacg
     8341 ctttccaagg attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag
     8401 ggcttcctat agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg
     8461 tagatgtaat tcaagttgga gctagaaaca tgcagaactt tgatttatta aaagaattag
     8521 gtaaaacaaa taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc
     8581 taatgtcagc agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag
     8641 gtataagaac ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg
     8701 tgaagaaact tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat
     8761 ggatggttga ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag
     8821 aagttcataa tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg
     8881 aatatgatga attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat
     8941 aatctataaa aggatggtta aacgtggaag aaaaactatt agtaaattta aaagaaaata
     9001 gctacaatat atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa
     9061 tatattttgg aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg
     9121 ataaagtaaa aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat
     9181 ctggtgaaga aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact
     9241 ttaagttaac tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag
     9301 gtggatttgc agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt
     9361 tattagcaca agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca
     9421 aaaatttagt gggaagcttt taccaaccaa aagctgtgtt tatcgaccca aatgtattat
     9481 tatcacttcc agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta
     9541 taaaagacaa aacttttttc aataaacttt taagtttaaa aaatagagaa gaagtaatgc
     9601 aacatataga atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg
     9661 atgaaaaaga ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta
     9721 ttgagaaata ctacaatttt acgggttata cccatggaga agctgttgcc ataggaatgt
     9781 ataagataac tttagaaagt gaaaagatag gaattacaaa aaaaggtaca tcagaactta
     9841 taaaatcaat tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat
     9901 ctatagtaga tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac
     9961 ttttaaaaga tattggagag agtattatat ataatactac ttctaaattt tttcaggagg
    10021 taagataatg ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc
    10081 accatcaaaa agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag
    10141 tagaatttct aatatagatt tttctgatga tataatagct actataagag caatgacatc
    10201 attaggagct attatagaaa agaaagaaga tattttagaa atttcgggaa ttttttctaa
    10261 agaaggtatt ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa
    10321 tgaatcaggc tctacattaa gatttttagt acctatatct ttagcatttg atggtgtaaa
    10381 aaggtttata ggtagaggta atcttggaaa aagaccactt gatacttatt atgagatatt
    10441 tgacagacag aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcagg
    10501 taagcttaaa ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg
    10561 tctattgttt atattgccta cactagagag tgattcaaag ataataatta ctactgagct
    10621 agaatctaaa ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat
    10681 tataaacaat aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga
    10741 ttataaagta gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg
    10801 agaagatatt agcatattag accttaaaga agattctctt caaggggata gtgaagtagt
    10861 tgaaatttta tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat
    10921 aacaaatggt ttaaatagta ctttgataga tgcatctcaa tgcccagata taatacctgt
    10981 attatctgta gtagcatcct taagtatagg gaggactaca ataattaatg ctggtaggct
    11041 tagaattaaa gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc
    11101 aaatatagag gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg
    11161 agtagaggtg tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg
    11221 tagatgtgat aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca
    11281 tttttttaaa gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata
    11341 atttaaaagt atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg
    11401 gtcttccttc ggggattgaa cttgaccttg ataagattga taaagaaatg aagagaagag
    11461 cacctggaaa aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa
    11521 gtggatattt taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg
    11581 atactcgttc aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt
    11641 tcacaggtaa tgtgagatat tctggattta atgactatag agggggaggt catttttcag
    11701 gtaggataac agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa
    11761 aaggaataga gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg
    11821 attatgtaaa tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac
    11881 ttgatttatc aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaagag
    11941 attcagttgg tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc
    12001 cattttttga ctctgttgaa tctactttat cacatttatt gttttctgtt cctgccgtaa
    12061 aaggtgtaga gtttggttta ggttttgaac ttgcagatat gtatggctct caaagtaatg
    12121 atgaaatgta ttatgaagga aatcaagtta agagcaaaac aaataacaat ggagggatta
    12181 taggagggat tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactcctt
    12241 caattagcag acaacaaaac acagttaata taaaagataa aaaggacgat attttatata
    12301 ttaaaggcag acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa
    12361 cagccattgg aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata
    12421 tagaaatgag attgacaata tagataaaga gattattcaa ttatttgaaa agagaatgaa
    12481 tacagttctt gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga
    12541 tagagaagaa aaagtactaa gtagagccat agataattta gataataaag catactcaca
    12601 agaaacggta cagtttttta attctataat ggaaattagt agaggattac aaaaaagatt
    12661 gatagataat gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaaatt
    12721 tgatttgtca aatgtaaata aatataagtc tttaaaagat gaattaaata aaaaaaatat
    12781 cctagtagga tttccaggaa aatcaggttc atttacagaa gaagcactaa ataaattctt
    12841 caacaaaaca atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa
    12901 aaataaagaa ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc
    12961 tgaaacttat gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa
    13021 aatagaccaa aatttaatag ggataaaaga cacaaaactt gaagatataa aagaaatata
    13081 ttctcatcca caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa
    13141 attgatacca tttaataaca cagcaacaag tgcagaactt gtaagaaaac ttcaagataa
    13201 aacaaaagca gcaattgcaa gcaaaaaagc tgcaagtatt tatgaattag aaataataag
    13261 tccttgtata aatgatattg caaacaacta tacaagattt gtagtaatca gtaatcaaat
    13321 acacattgaa gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg
    13381 aaaactttat aaagtacttg gatactttgc tgaaaataat ataaatatga caaaaataga
    13441 gtcaagacct atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc
    13501 tatatacaat tctaaagtgt acgacttatt agaattgata gaacataata cagcatattt
    13561 taaatttatg ggagtatata aaaataaaac tgaatagata atataaattt ttatttaatt
    13621 tataattatt aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc
    13681 acagtgtttc acctcaaatc cacaagagaa tattcgagat attgaatatt gaaagtgctt
    13741 ataaaaactt tgaaattcca aaagaagata tatctaagct agacggtgct ataaaattat
    13801 taggaataca aggagtaaat gttactgtac catataagga gcgtattatg aaatatcttg
    13861 attttatatc accagaggca aaacgtatag gagcagttaa tacaatttta ctcagagaaa
    13921 atatgttata tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta
    13981 atattgatgt tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg
    14041 ctcttacata ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg
    14101 atgataaaaa attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata
    14161 taaaaggaga tataatatta aatgctacac cagtaggtat gtaccctaat gtaggaattt
    14221 caccagtgtc aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc
    14281 ctggagaaac agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat
    14341 tatatatgct ggtagggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag
    14401 ataatagtat tttagatgtt atatataatg aactaaaatt agaatttttg taagggagat
    14461 tgtcatgata aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa
    14521 aactacaatt ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga
    14581 ttatataatt caaatatctc aaaaaagtat agcagagttg ttttcagaag gagaagatat
    14641 ctttaggaac tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat
    14701 atctacaggt ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg
    14761 tataataatt tttattgata gacctataca aaagattttg gaagatataa atattaattc
    14821 aagaccatta ttaaaaaacg gaaaagatag attgtataat ctttataatg agagaataaa
    14881 tctttataaa aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt
    14941 atataatatt acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc
    15001 tagggggaaa taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct
    15061 tttgctaaag cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta
    15121 gaaactttaa aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa
    15181 gagttattta aaaaagctga tttaattata ctatctatat atcctagatt agttgtagat
    15241 tttctaaata ataataaaaa cttttttaaa aaagggacta taattactga tactacagga
    15301 ataaaagaaa cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata
    15361 tttggacatc caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt
    15421 tttaatggcg caaattatat aattactcca actggcagaa acaacataaa aaatttagag
    15481 ttagttgaaa atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa
    15541 aaacatgatg agattatagc ttttacttct cagttacctc atgttatggc agttgctctt
    15601 ataaatagtg atgaagaagg tagagataca ggtaaattta ttggagatag ttatagagat
    15661 ttaactagaa ttgcaaatat gaatgaagac ttatggagtg aacttttttt aggaaataga
    15721 gataatttac taaaagttat agaaaacttt gaatcagaag taaattta
//
LOCUS       DAEPOG010000092        15747 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEPOG010000092 DAEPOG010000000
VERSION     DAEPOG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.092"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            167..2479
                     /locus_tag="KR432_002300"
     CDS             167..2479
                     /locus_tag="KR432_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0640830.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KR432_002301"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KR432_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBH0640831.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="KR432_002302"
     CDS             2916..3344
                     /locus_tag="KR432_002302"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBH0640832.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="KR432_002303"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="KR432_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBH0640833.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4411..4848
                     /gene="spoVAC"
                     /locus_tag="KR432_002304"
     CDS             4411..4848
                     /gene="spoVAC"
                     /locus_tag="KR432_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBH0640834.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5002..6015
                     /gene="spoVAD"
                     /locus_tag="KR432_002305"
     CDS             5002..6015
                     /gene="spoVAD"
                     /locus_tag="KR432_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBH0640835.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6029..6391
                     /gene="spoVAE"
                     /locus_tag="KR432_002306"
     CDS             6029..6391
                     /gene="spoVAE"
                     /locus_tag="KR432_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBH0640836.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6574..7590
                     /locus_tag="KR432_002307"
     CDS             6574..7590
                     /locus_tag="KR432_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0640837.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            7965..8807
                     /locus_tag="KR432_002308"
     CDS             7965..8807
                     /locus_tag="KR432_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0640838.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8822..9286
                     /locus_tag="KR432_002309"
     CDS             8822..9286
                     /locus_tag="KR432_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640839.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9273..10445
                     /locus_tag="KR432_002310"
     CDS             9273..10445
                     /locus_tag="KR432_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0640840.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10489..11424
                     /locus_tag="KR432_002311"
     CDS             10489..11424
                     /locus_tag="KR432_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBH0640841.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12223..12621)
                     /locus_tag="KR432_002312"
     CDS             complement(12223..12621)
                     /locus_tag="KR432_002312"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0640842.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(12920..15613)
                     /locus_tag="KR432_002313"
     CDS             complement(12920..15613)
                     /locus_tag="KR432_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0640843.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
BASE COUNT         5752 a         2055 c         2842 g         5098 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataaat atattactaa aataaaatgt aataactttt
     4201 tatgttaagt ttttaaatta ttaaaagata ctaaaataat aaaaagcatt aaaaaagtaa
     4261 aaatataaaa acaaggtgtc tttatggaaa aatagatttt ttctttatta ggcatctttt
     4321 ttattttttg accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt
     4381 aacaaataat actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac
     4441 caaataagcc caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga
     4501 attatttgta tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat
     4561 aaactaggtg ctagttctgc aacatcaata acattaatat ttataggggc atttttaact
     4621 ggattaggag tttacgacct aataggaaaa agagctggag ctggttctat aattccaata
     4681 acaggttttg ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta
     4741 ttaggtgtag gtgcaaactt atttaaaatt gcaggaccag ttttagttta tggtataggt
     4801 tcttctattt tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt
     4861 taatttaaat tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa
     4921 ctaattgaaa aataattgtg cagatttaat taatattaaa aatttgaata gtaaaatgca
     4981 aaattaaaaa ggtgtgaaaa tatgaaaaat aaaagaattg gaaaaagaac agtcaagtta
     5041 gaaaataaac caaccataat atcaactgga actatagtgg gtcctaagga aggtgaagga
     5101 ccactaaagg actattttga tatgattatg actgacgatt tatatggtga aaagacttgg
     5161 gaattagctg aaagtaaaat ggtagagact gcatcccaac aagctatcca aaaggcaggt
     5221 aaaaaattgt cagatgtaaa ttatatgcta ggtggcgatt taataaatca aatagtgcca
     5281 gcatcatttg cagcaagaga actggctatt ccatttttag gtatatatgg agcttgttct
     5341 acaatggcag aaggtctttg tataggttct atgcttatag atggtggttt tgctgattta
     5401 gtactgtcag gtacatcaag tcactattgt acagcagaaa gacaatttag attcccactt
     5461 gaacttggaa atcaaaaacc aatgacagct cagtggacag ttacaggagc aggaagtgta
     5521 ttattagctc ctaatggaga cggtccaaaa gtaaagtatg ttactgttgg taaagtaata
     5581 gatgagggta ttgatgatgg taataatatg ggaccagcta tggctccagc agcaatagat
     5641 actatctact cttactttga agatactaat gatgacccaa atagctttga tataattgct
     5701 actggagatt taggtactct tggaaaacag atagcagaag actttctaaa agaaaaaggt
     5761 gtagatatat caaaagttta tactgactgt ggtatagaga tgtttaacct taaagaacaa
     5821 gatgtccact gtggtggaag tggatgtggg tgttcagcta cagtgtttgc aggatatata
     5881 tatgataagt tgagaaaaaa agaatttaac aaagtaatgc ttgtatcaac aggagcacta
     5941 ctttcaccga caagtacact acaaaagcag acaataccat gtgttgcaca tgcagtagta
     6001 atagtaaatg agtaggaagt gatataatat gttaatggac tatgtaagag tatttattgt
     6061 aggtggaatt atatgtttaa tagcccaagt aatgatggat tatttcaaaa tgcaaactcc
     6121 atatgttatg gtaacgtatg taacaactgg tgtagtttta acttttctag gactttatga
     6181 gccattagtt aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc
     6241 acttgctaca ggagtaataa aatcaattca aagtgatggt tttttaggta tttttttagg
     6301 aggtacaact gctacagctg gtggaatagc agcagctata ttctttggat atattatgtc
     6361 agtagtattt actccaaaag caaaaccata aatctttatt aaaagaataa taagtttttg
     6421 aaataataac tttttataaa gcaggtggat atcttttaga ttccacctat tttttatata
     6481 aagaagcgtt tacataattg aagttgacaa ttatttacaa catagtgtag tattatatta
     6541 tcaaaataaa atgaagtgaa gtaggggata aagatggtaa ctattaaaga tattgcaagg
     6601 aatctaggta tcagttattc aactgtttct aggtgcttaa acaataatcc taatgtatct
     6661 gaaaaaacaa aaaagaaagt agtagaagag gcaaatagac tagggttcca ttttaatgta
     6721 aatgcaagaa atttagcaaa aaaagaaact aacaggatag gggtaatctt ttcaaataat
     6781 tttaatcatc aagatactag aaagtttttt agtgatataa tggatagttc tataaattct
     6841 attgagacta ataaatacga ttttataata cagcctaata ataacattag tggtgatagt
     6901 aatgtatata aaatggtaaa tggacaaatg gttgatggat tagtaattgt gtctcaatca
     6961 ataaaaaaag aagagtatga ttttttaaaa gacaataatt ttccacatgt atttatatat
     7021 tttaaaccat cttttgtagg agaagtagac aatttctttt gggacgataa cgtgtatgga
     7081 ggttatatag ctaccaagca tttaattgac catggtcata aagacataat aacagtaact
     7141 tcagatgata ggagttcaaa aatgcatgaa gatagaacta agggatattt aaatgctatg
     7201 gaagaggcaa atttaaagac cgaagtcatt aaatctaaaa tggattttga atctcaagtt
     7261 gaatttttag aaaaatatat tgataaaata aaaaaagctt cagctatatt tgtacaacaa
     7321 gatgtacctg cgatttctat aatacaagag ttaaaaacta cttatggtat aaatgtacca
     7381 gaagatattt ccataatagg gtataataat attgaattaa tatcatattt taggtcgcat
     7441 ttatcaacaa tagatgaccc aagagagcaa gttataaaaa atggagtaga tagtttagta
     7501 aatataataa ataaaaagag tacagaacac attccaagaa aattatatcc aaggttaata
     7561 atacgaaata gcgttaaaag aataaagtag taagctttta agtatcaaaa gtttattaaa
     7621 aatattatag attatgtgaa gttttttaac aaacaatata aataaaaatt aaaaggagta
     7681 ggtgtatata agtacattgt actcctttat ttattgagat agtttttata atatgaatga
     7741 ataattttat tcaattattt gaaaatccgt tattttaaca atataaatta ataaattatt
     7801 tgtattttat atcatgaatc tataatattt ttttgattca taactaaaag tatataaaaa
     7861 tgatgaataa atatatatta tacattatca atgataggtt atataataaa atcactaaca
     7921 attatattat aagaaaatta taaaaattac aaggaggtat agaaatgaaa gaaaaagcag
     7981 ggattagaaa tgccataata gcagccttta taatcacaat attggcagaa ttaattggac
     8041 ctatttcatt taaagttatg ggaataaatg taactttact tagtatacta tgggctatat
     8101 tcataggaat ggctgtttct cctcatttat tagggagagt tataccagcc ttaaaaaagt
     8161 ttatagggga taatgaaata aatgtatctc catatttatt atctttgaca ctatatccat
     8221 taggaataat gtttggaatt aatgcagggc ctaaagttgg tatattactt caagcaggac
     8281 cagcactttt attacaagaa tttggtcata tgggtacaat gttgatagca ttacctgttg
     8341 gagtagcatt aggtcttgga agaagtgcat taggtggtac tttttcatta tgtcgtgaca
     8401 cagcactagg cattattgga gataagtatg gattaaactc accagaaggt ataggaacac
     8461 taggaactta cataagtggt agtatatttg gaacattatt attttcattt ttagcaccag
     8521 tgggtgtact tttaggattt catccatatg ctttagcaat ggcatcagga atgggtagtg
     8581 gttcaatgat gggagctgca acagcatcat taatgaatac ggttccacaa atgtcagagc
     8641 agattttagc atattcagct actagtggat tgattacagc tgttactgga atatatatag
     8701 aattattctt atctttacca gtagccaact actattatag caaagtagct ccagttattg
     8761 aaagatttag aggaagaaaa acaagaagaa caagtaataa attgtaaagg aggatatata
     8821 tatggaaaaa acttttaaga ctattatgga gcagataaaa atagtattgt ttatatatat
     8881 tattacttgc tttggacaaa tgatagcact taagactcag gctcaagatt tgttgattgg
     8941 aagtatatct ggatttttgt tggtgataat tgctattata ataaagaatt ttgttaaaaa
     9001 acctaattta ccaggttttg catgggctac attagttgca ttcgttttat cgcttccaat
     9061 atctccagtg ggggatatca tagcaacaaa tgtagggaaa atcaatttta tgtctacagt
     9121 aacaccatta ttagcttttg caggaatctc tgtaggtaac caattagata tattaaagac
     9181 tttgagttgg aagttagtag ttatatcatt tatagttatg acatctactt actttggttc
     9241 tgcttttata tctcaaacag ttttaagatt aaatggaatg ctttaaggag gtagtaaata
     9301 tgaatgaatt aaaagcaaaa attaaagagg aaataaagtt attatctgaa gagaaaaaga
     9361 catcatttga aaaagtttct gactatttat tttcaaatcc agaattagca tttgaggagt
     9421 ataagtcaca aaaagcatta tgtgatttgt tagaagaaaa tgaatttaat gtaactaaag
     9481 gtgtaggagg attagaaaca tcttttgaag ctgtgtattc aaatggaact aatgggaaaa
     9541 cagttgcttt tctagctgaa tatgatgcac ttccaggaat gggacatgca tgtgggcaca
     9601 atatcatagg gacaagttct gttggtgcag gtatcatatt aaaagaaatc atgaaaaaac
     9661 ataatataga agggactgta aaggtttttg gaacacctgc agaagaaaga gtaggtggaa
     9721 aaataactat gattaaagaa ggtgtattta ataatgtaga tgctgcatta atcttacatc
     9781 caagtgatgc ttcaatgcct gatgatatat catttgctca agttaattta aaatttgatt
     9841 ttactggtaa ggcatcacat gctgctgctt ttccttggga agggaaaagt gccttaagtg
     9901 gagtaatagc actatttaac agtgtaaata gcatgagatt acatcttaaa gattatgcaa
     9961 gagtacatgg aattataact gatggaggta gcatacataa tataattcct gaaaaatcta
    10021 cagctatatt taatgtaaga gccttaagca tagaatattt aaatgagatt tgtgaaatgc
    10081 ttaaaaattg tgctaaagga gctgcaattt ctactggaac taatgtagaa atagttcaat
    10141 tagatgaaat atacaaagag attaagaatg attctgaatt agtgaatata gttagacaaa
    10201 attttgaagt tttgggtgaa gactatgttg aaagagattt aagtcaaggt ataggttcaa
    10261 ctgatacagg aaatttaact catgagatac ctgcaataca agcatatata aaattaaaag
    10321 aaaatactgc aactcataca gatgaatttg ctgttgcagc tggtggtgaa gaaggcaaag
    10381 tagcacttat aaaagcaatt aaggtgctag caatgtgtgg agtagatatt ttgtatagta
    10441 aataatatat aattttaaaa tatattaaga aaggaaatcg gattagacat gatattaaaa
    10501 caagtaatag aggtttatga cattttagat aaagcaaatg ctaatgggga agaagtaaaa
    10561 acttacttac aaggttatgg tgaagtagat gtaactgtaa aagaattgtc ttcaagtaaa
    10621 ggttcaacag atttagtaaa gcttacaatt cctgggaaaa atggaaaatt aaaaggtgga
    10681 gatgctccta ctcttggtat tttaggtaga ttaggaggga ttggagcaag accagaagtt
    10741 ataggttttg tctcagatgg tgatggagca ttggtagcaa ttgcagtggc tgctaaactt
    10801 ttggatatgc aacgaaaagg ggacatatta gatggtgatg ttgtaatttc aactcatatt
    10861 tgcccagatg ctccaactaa agaacactat cctactcctt ttatggattc tccaattgat
    10921 atgatgacta tgaatgaaaa tgaagttgat agtagttgtg atgcaatact ttctatagat
    10981 actactaaag gaaatagagt aatcaatact agaggatttg caatatcgcc aactgttaaa
    11041 gaaggctaca tattaaaaac tagtgataac cttttagata ttatgcagac tgtaacaggg
    11101 aaatctccat ttgtattccc tttgtctatt caagatataa caccatatgg aaacaatctg
    11161 tatcacttaa atagtatttt acaaccagca gtagctacag atgcaccaat agtaggagta
    11221 gcaattacaa cagagatgcc tgttgctgga tgtgctacag gtgcaactaa ttttagtgat
    11281 ttagattcag ctgggagatt tgcaattgaa gttgccaagc tatttggtag aaataagtgt
    11341 gatttctatg ataaagaaga gtgggaaatg cttattaaga gatatggcaa attaaataga
    11401 tttcaaacat ttggtataca gtaattagaa aaatattaag tttatataat ttgtgataca
    11461 actatttaag aagatataaa caattaggaa taaaagtaag aataaagcac ttagttaaaa
    11521 actttaaaaa aaaatattta tttgtttata taaaaaggag gattctggtt attgggattc
    11581 tcttttttat gaatacaaat aatatattgt aattataaaa aaaggagcgt ttcaaagtga
    11641 tttttttaat caccttgaga caactcctta tattttttat ttaaattcta ttttttattg
    11701 actgtatagc tattttcacc atcataaggt ggcatgacct tgaacagcgt ggttttgtaa
    11761 agttgggcac cacaaaacaa aaaatcaaca agcccttgtg tggtctgctg acgtgtttac
    11821 tatactatac tttcccaaaa accgcaagca ctatgggagg ctataaggcg gcgaccattc
    11881 aggggcgatc ccctcgaaac cgggataggg ttcccatgtg ggacataata aagagcgccc
    11941 cggaggtatc aactaccatc cggggcgctc tttattatgt cccgtgggaa ccatgcccac
    12001 gaagcagggc gtacaacgga cagataggtc ctaccctcct gacagactgg aatctcaatc
    12061 ccatcgaaaa caatcataac aatcatggca agattatata cactcctgta aaatattttt
    12121 gtgtgtacca tgaagtactg cttgccacta ttgagaacgg ataaaaggga agtacgtatt
    12181 ataccaataa ggtgaacgaa acaaaatact catttgataa agttaaataa acctgtcgga
    12241 accccaccaa tccggcaaga gctcactcaa tttaacagtt ttttctgttt cgacattaac
    12301 taaaatctca atacaagcac tatccttatc aagttgcatt aaatattcac gacaagcacc
    12361 acatggtgag ccaacactcc cattgctcat cacacaaact aatttagtaa ttttactttc
    12421 gccatttgtt atcatattgg cgatagcatt tctttctgca cacattccca atgtacaggc
    12481 tgtatcaata cacacgccag tataaacatt tccgctgtcg gtaaggattg ctgcggcaac
    12541 cccaccagca tcgataaaag gtgaaattgt gcgtggattt aattttcccc tagcattatt
    12601 ataaagttcc ttccatttca tcaggttgat cctcctgttg tatccatata aataatcttt
    12661 tatacgcctt ttccgcctaa ggcacacaac acattactcg cttttttcat tctaatattc
    12721 gtgcgtgaaa atgtcaaacg gtttacttcg tttcattact tcatactgct gttcataacc
    12781 ctgtttgttc acttgggcac cattcgagca ccaatttacc cgtttcaggg ttgtagcgaa
    12841 cacagcagaa atgctcattt catgggcttt tctggctaga aaaaaaattt tatcgctgcc
    12901 agatatttac tattttttat taagttgctg gagttccacc accatcagga ggagtagtag
    12961 agccaccacc attatttcca ccattatcag gaggagtagt agagccacca ccattgtttc
    13021 cgccattatc aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag
    13081 tagtagagcc accaccattg tttccgccat tatcaggagg agtagaaccg ccaccattat
    13141 cacctgtgtt attattgtta ttattatttt cttctggttg gttattgtct ggtttttttg
    13201 tatcttcaga tgatggttga gaacttactt tagtaggagc agtaccttca ataaatgctg
    13261 ctttagagcc agaagattgc tttcctccat cctctaaatt aatttttgta aaatatacat
    13321 ttttaggaac ttcaaattca gtaaccgtta aattagcatg tattttttcc ataatttttt
    13381 cccatagttt tgcagtacta gtacttccat gtggtacttc tctacgtttt atagtgttac
    13441 cagaatcatc ttttctacca gcatcatctc caatataagt tgcaccaaca taataaggag
    13501 tgtaaccgac aaaccaagca tcatatgcac tatttgtagt acctgtttta cctgctacag
    13561 gcattccctt tggaagtgta gcaacaccac cagtaccttc agtaattacg ctttctaaca
    13621 tatctgtaac aacataagct acttcagggt ctacaacttt atgttcttca ggagcatttt
    13681 taactaataa ttgaccatca aaggtagtta tagtagtaaa aatagaaggt tctacataaa
    13741 caccttgatt tgccaatgta ccataagcag cagccatttg aagtggagta ataccattcg
    13801 ccataccacc tagtgtcaat gatgggaatt ttctatcact tgcttcagat gaacctgagt
    13861 gattattctt taaagttgtt ataccaaagt tctttaagta atccatcatt atatctacac
    13921 attcatcata agagtctcca agcatttcag cagttttgac agctatcgtg tttgaagatt
    13981 tagcaagtgc tttacgtaaa ctcatagaac catgaccagc agtagtagtt cttggattcc
    14041 atttattatt ttcttcaaac ttatatcctc ctcgagaatc acttagagca gttgattgtg
    14101 ttatttttag ggtatcaata gcaggtgtat atattgctaa aggttttata gaagaacctg
    14161 gctgatgagg gttagtagca cgattcaaag tttttctacc acttatattt cttcctccag
    14221 ctagtgctct tatttcacca tttttataat ctaagattac cattgcagct tgtggttggc
    14281 taactccatc agggccgacc atatgtcctg ggaaattgct atttctatca aattcttctt
    14341 ctaaagcatc ttgcatttta gggtctatag ttgtgtttac aattagacca ccattgtaga
    14401 ataagttttg agcttcatca tttgtatgtc cttgttccat taaagcattt ataacttcac
    14461 tttcgataag gtcttctaca gatgaagaaa ctttatctga tgattgaggc aattttattt
    14521 ctattttagc tttgattgct tcatcatatt cactttgttt tatatatttc aattctaaca
    14581 ttttctttaa aacaatttct tgtctttttt tagcttcagg gttacttaca gcctttctaa
    14641 caactaatgt acctgctttt aattgtctat aagtatcatt agatataagc tcccaagatt
    14701 ttattttttc aatcatatca aagtccactt gagtagggtc atctaaatca tcatcagttg
    14761 tgttaacaaa aaataataat ttattttcta aatcctcctt agtttcattt ccctctaact
    14821 tagaagtttt ataggctgaa aatcttgatg gatttttagt tgaaccagca agaagtgctg
    14881 actctgctag agtgaggtca gctgcagact tatcaaaata accacgtgca ccagcctcag
    14941 cacctgctag acctctacca acgaagaagt tatttaaata agtttctagt attttatctt
    15001 tacttactgt tttactcatc tcatgtgcat agtaaatatc ctttatttta cgaggtatag
    15061 tttgctgttc agttgttaac agcattttgg atacctgcat aggaatagta ctaccacctt
    15121 gcttagtacc tgtaagggtc tttaatactg acctaaataa acctttaata tcgacaccct
    15181 tatgctcata aaaacgctca tcttctattg caattacagc atgttgaaga taaggggaaa
    15241 tcttatctaa tggaacgggt gtctttttat ttacacttgg agctgtagac aatgtttttc
    15301 cattagcgta tttaatagta gttgtttggt tcgtctgttt atctaaaaca gcttctgtaa
    15361 ctggagttac atctcttagt gacgcaaata ctagaccagc agtacctgct gtaccaacaa
    15421 ctagtaaaac taaaaatact atcccaaata ctcttagttt tttaaattta tcgctttttt
    15481 tagatttttt cttgtttttt gttgaacgag tcttgttagc tgaagttcta ttaattggtt
    15541 tatttgtatt accagatgag ctaacttttt ttctcctgat tttatttcca tttttatcat
    15601 tatcgttatt cataagattt taacctcctt acttaatgca atattataac acaaatagga
    15661 gtagttttgt aattaataga aaaaatgaat ttgctataat attctgtaca ttgtatatgt
    15721 ataaacgtaa tatattaaaa agtctat
//
LOCUS       DAEPOG010000093        15668 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEPOG010000093 DAEPOG010000000
VERSION     DAEPOG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.093"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(404..1501)
                     /gene="ychF"
                     /locus_tag="KR432_002314"
     CDS             complement(404..1501)
                     /gene="ychF"
                     /locus_tag="KR432_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH0640844.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1817..3082
                     /locus_tag="KR432_002315"
     CDS             1817..3082
                     /locus_tag="KR432_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0640845.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            3097..4383
                     /locus_tag="KR432_002316"
     CDS             3097..4383
                     /locus_tag="KR432_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0640846.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4780..5526
                     /locus_tag="KR432_002317"
     CDS             4780..5526
                     /locus_tag="KR432_002317"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH0640847.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            5556..6491
                     /gene="rsmH"
                     /locus_tag="KR432_002318"
     CDS             5556..6491
                     /gene="rsmH"
                     /locus_tag="KR432_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH0640848.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            6977..7324
                     /locus_tag="KR432_002319"
     CDS             6977..7324
                     /locus_tag="KR432_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640849.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            7336..9315
                     /locus_tag="KR432_002320"
     CDS             7336..9315
                     /locus_tag="KR432_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBH0640850.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            9482..10855
                     /locus_tag="KR432_002321"
     CDS             9482..10855
                     /locus_tag="KR432_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH0640851.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            10896..11864
                     /locus_tag="KR432_002322"
     CDS             10896..11864
                     /locus_tag="KR432_002322"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH0640852.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            11883..13238
                     /locus_tag="KR432_002323"
     CDS             11883..13238
                     /locus_tag="KR432_002323"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH0640853.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            13290..14420
                     /gene="spoVE"
                     /locus_tag="KR432_002324"
     CDS             13290..14420
                     /gene="spoVE"
                     /locus_tag="KR432_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBH0640854.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            14438..15664
                     /gene="murG"
                     /locus_tag="KR432_002325"
     CDS             14438..15664
                     /gene="murG"
                     /locus_tag="KR432_002325"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0640855.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
BASE COUNT         6169 a         1685 c         2864 g         4950 t
ORIGIN      
        1 aaatatacta ttataaaaac ataaatcatt gttaggatat tattttagga attattgggc
       61 aaagtttatt aatatgtagt attttttcca tttgaatgtt tttgaggtaa gagtatatgg
      121 gattatagca aaatacttta gatagtaata atagtaattt ctataggtgt gttatacata
      181 ttttaaaaaa taaaaagata atttataaat ttaaatgtta tatatgtaag taattttatt
      241 atatcaaaaa aagagttttt cataataatt tatacaaatc atttgaaata ctcttctttt
      301 atttttaaat tctatataat tattataaga taatattgta ttattataat cttagttttt
      361 tagttattat atataatagt caaagtgtat atgattaatc attttaaaca ttaaatctaa
      421 ataatattac atctccatct ttaactatgt attctttacc ttctaattta accaaccctt
      481 tttctttagc agcagccata gtaccatgct caaccaaatc attaaaggct attgtttctg
      541 ctctgataaa tcctctttct atatcagaat gtatttttcc tcctgcctga ggagctttac
      601 ttccaacttt tatagtccat gctctaactt cttttggacc agcagttaag aatgaaatta
      661 aaccaagtaa agcataagaa gattttataa gcttatcaag ccctgattgt tcaagaccta
      721 atgtttctaa aaactctttc ttttcttcag cagaatcaag ttcagaaatt tctgcttcta
      781 tttgtgcaca tactacaaca acttcggcat cttctgtttc tgcaaatgct ttaacttgtt
      841 gaacatattt attattttct ccattatcag ctaaatcatc ttctgataca tttgaagcat
      901 atattacagg cttagatgta agtaaatcca atgaatttac gaatgtttgt tcatcttctg
      961 taaattccat agttcttaca cacttacttt cttctaaagt agacattatt gattttaata
     1021 aatcaagttc agaacctaaa gttttatcag ccttagcagc tttttgagtt ttattaattc
     1081 ttctttcaag aatctctata tctgagaaaa ttaattctaa gtttatagtt tctatatcgc
     1141 gaagtgggtc tacagagcca tctacatgta caacattttc atcctcaaaa catctaacaa
     1201 catgaactat tgcatcaact tctcttatat gtgataagaa tttatttcct aaaccttctc
     1261 ctttagaagc acctctaact aaacctgcta tatcacaaaa ttctatagca gtaggaacta
     1321 ttttttcaga attatataat tcttgtagtt tgtttaatct ttcatctgga acagatacaa
     1381 ctcccacatt tgggtctatt gtacaaaaag ggtaatttgc agattctgca cctgcttgag
     1441 ttattgcgtt aaatagtgtg cttttaccta catttggcaa accaactata cctaatttca
     1501 tattaatcgt cctctctctt attctttttt atattcttta tataacttta aacaatcaat
     1561 ctataggatt gcaaaattgt tttaattata gacatacata ttgattatac atcatatata
     1621 tcaagcttgt aaactagact ttaaagcata ttgatttaaa taatatttgc atttaattta
     1681 aataaaaata ttgcttttag aaaataataa aaaggatata attaaataga aggtttttat
     1741 atgtgagtat atataattaa aaaataaata aatttcaatt ttaaaattga tgtttaaata
     1801 aaaataggag gtacatatgg ttaatactaa aaaaataata aatttaggta ataatattaa
     1861 tcttactttg ataaaaacag aaaagtttaa atcgaatctt ataagtttat atgttcagag
     1921 actattggat gaaaaagaaa ctacaaaaaa tgctctaata ccatcaataa tagcaagtgg
     1981 aagcgctaaa tatcctagtg ctagagcaat atccaataaa ttagatgatt tatatggttc
     2041 atctatggga gctgatgctg taaaacgtgg agaacgtcaa gtattgagtt ttaaagtaat
     2101 taacataagt gaaaagtatt tagatgagag tatatttgaa gaagtagtag aattttttaa
     2161 tgaagtaata aataatacgc ttgttgtaga tggtgggttt aaagaagaat atttaaatat
     2221 tgaaaaagag aatttaagag aaaagataca atcaataata aatgataaaa aggaatatgc
     2281 gcaagataaa tgtattgagg ctatgtgtaa aggagagaga tatagtgtaa gtgaatttgg
     2341 ttatgaagat gaaatagata gtataacttc aagagagtta tatgaacatt ataaaaacat
     2401 acttaaaaca tcacctatag atatagttgt agaaggtaat tttgatgaag ataaagttgt
     2461 agatataata agtaaaaatc taaaatttga aagagaagaa attataaata taccaagagc
     2521 agattttata aaaaatgtgg atgaggtaaa agttatagat gaacaaatgg aaataactca
     2581 aggtaagtta gttatgggtt atagagcaaa tgtggattat gcagacatag ataaatatta
     2641 tgctttagta gttgggagca atgtcttagg tggtggtcca cactctaaat tatttgttaa
     2701 tgtaagagaa aaagaaagtt tatgttacta tatattctct tcaatagaga agtataaaag
     2761 tatcatgttt atatcatctg gtatagaaac taaagattat gataaagctg tagaattgat
     2821 aaaaaaacaa gtagaaagtg taaaagcagg tgatatatct gatgaagaat tagaaaacag
     2881 taaacttgct ttagttaatt ctatgaaatc tataactgat aatataggtg gtatgtctga
     2941 ttttgttttc tctcagtcta tggcaaagac aaattctgaa gtacaagaca taatatctag
     3001 tatagaaaag gttacaaaaa aagatatagt tgaagctata aaaaatatag agcttgatac
     3061 tgtatacttc ttgagaaatt aatggaggag atgtctatgg aaaaaatagt taatgatata
     3121 ttgaaagaag aagtttatta tgagaaattg caaaatggat tagatgtata ttttatgcct
     3181 aaaaggggtt ttatgaaaaa gtatgctatt ttggctacta attatggttc aaatgactta
     3241 gaatttgtac caattggaga agataagaaa attagagtga atgaaggtat tgcacatttt
     3301 ctagagcata agatgtttga gcaacctgat ggaggagatg catttgataa attttcaaaa
     3361 ttaggagtaa atgcaaatgc atttactaat tttacaatga cagcctactt attttctgct
     3421 actgaaaatt tttatgagag tttagaacat ctaattgact atgttcaaac accttatttt
     3481 acagatgaaa atgttgaaaa ggaaaaaggt ataatagcac aagagataaa aatgtacaat
     3541 gatgaccctg attggaatgt atactttaat tgcttgaagg caatgtatgt aaattatcct
     3601 gctagaattg atatagcagg tactgttgat agtatatata agataactaa ggaagagcta
     3661 tataaatgtt ataatacttt ctataatcca ggaaatatgg ctttatttgt tgttggtgac
     3721 ttagatgtag aaaaagttat agatgtaact aaaaagtcaa acaattacaa agtagacaaa
     3781 ctctctaaga gtatagaaag attttatcca gaagaaccag aaagtgtaaa agaaaaagag
     3841 gttatagaaa aattccctat atctatgcct atgttcaaca taggttttaa agatagtaat
     3901 gttggtttaa aagggaaaga acttttacgt aaagagattg taactgatat acttgtaggg
     3961 atgttattta aaaaaggaag taagctctat gaagatttgt atatgcaagg tttaataaat
     4021 gaaaattttg gagctggatt ttcgtctcaa gtggattatg cttttagcat aatagctgga
     4081 gattctaaag agcctaaaaa agtaaaagaa ataattcttg attatataga aaaatcaaaa
     4141 aaagaaggat tatcaaaaga agagtttgag agaactaaaa agaaaaaaat agggtcattt
     4201 attaagtgct ttgattctat aaactttata ggtaatagtt ttatatctta tgtttttaag
     4261 gatataaatc tattggatta tttagatgtt attaaagata ttacttttga ggaagttgaa
     4321 gaaagattaa aagagcattt taaagaagag tattgtgtta tttctattgt agaacctaaa
     4381 tagaaactta atcataataa gaataactaa ttaagtatta tttatgtttt taaatgttta
     4441 ttttggtaaa acaaatttat aaaatatatt tatataaatt gaggtaaaaa tttaagagct
     4501 atagataagg aatagaagag gttgatgtat atatgcatca atctttttga ttaaaaaata
     4561 tctatgtaca cttgtaactt tagtcatgag agattcagtt taaaaaattt tatagtaatt
     4621 atattagtat aatatagtgt agggatttgt agaataaatt ttaatatttt ttgtagaatt
     4681 aacattaatg tgttatcata atttagtata attacaatta attgtaatta tagcatattg
     4741 ctttggaaat attttcaatt aatacaaaga ggtgacgtta tggatagagt agcatttaca
     4801 ctgtttggca tagacatcat gtggtatgga atacttatgg catgtggaat gattttagga
     4861 actttaatag caataaaaga agcaaaaaga gtgggaatta aagatgatga tgtattaaat
     4921 atagctataa tagcaattcc agttggacta atctgtgcaa gaatctatta tgttgtattt
     4981 aattggagtt actatgctca aaatatgtct caaattttta attttagagg tggtggactt
     5041 gctatacatg gagggttaat tggtggaata ttagctggtt atatatacac taagattaaa
     5101 aatattaact tcttaaaaat ggctgataca gttatacttg gaatgccttt agcacaagct
     5161 attggaagat ggggaaattt tattaatgga gaagcacatg gaggtgctac caatttacct
     5221 tggggaataa tggttgatgg agtaaaggta catccaactt ttttatatga gtccatatgg
     5281 gattttggaa tatttatagt tttattacta tttagaaaaa ataaaaaata tgaaggtcaa
     5341 gttatagtta cttatataac tttatattca ataggaagat tttttataga gggattaaga
     5401 acagatagtt taatgttagg accacttaga atggcacaag ttataagtct aattggagtt
     5461 attggtggca taattgcaca tgtttattta tcaaagaaaa ataaacataa tattagtgaa
     5521 gaatagttta aaaagtgaat ttaagggagg taactatgga atttcatcat gtatcagttc
     5581 ttttaaatga gtgtatagag aatttaaata taaagccaga tggcgtgtat gttgactgta
     5641 ctatgggagg agcaggacat tctaaagaaa tagtaaaaaa actgtcagat aagggattat
     5701 ttatagggtt tgaccaagat aaaaatgcta taagcacagc aaaagagaga ttgagtgaat
     5761 atgaaagtag agtgaagttt gttcacagca attttgaaaa tataaaagag gaattagaaa
     5821 aaataggagt ttacaaaatt gatggtgtat tagctgattt aggagtttca tctcatcaac
     5881 ttgatgaagc tgatagagga ttttcttata tgcaagatgc accacttgat atgagaatgg
     5941 atgttaggtg tgagttttct gcatatgatg ttgtaaatac ttatacagaa gatgaattaa
     6001 ctaagataat aaaagattat ggagaagata actgggctaa gcgtatagct aaatttatag
     6061 ttgaagaaag agcaaacaaa ccaatagaaa ctacaggaaa attagtagat ataattaaaa
     6121 aagctatccc taagaaagct agaatagatg gacctcaccc agctaaaaga acttttcagg
     6181 caatacgaat agaagtaaat aatgaacttg gagtaattac aaagatgata aatgatgctt
     6241 cttctataat gaataaaggt ggaagaatat gtataataac attccattca ttagaagata
     6301 gaatagtgaa aaatgcattt aagcatttag catcagattg tatatgtcca caacatttac
     6361 ctatatgcca atgtgataaa gaatctgagg tgaaaataat aactagaaaa cctatattac
     6421 ctagtgagga agaaatagaa gtaaatccac gttcaagaag tgctaagcta agagtagcag
     6481 aaaaaatata agtagttata aggctaaaga ataatcttat atataaaata agattactat
     6541 aattaattaa aaagtattat atattttgtt gaactggtaa agcaattaaa aaacaattaa
     6601 cactataaga aatgttcttt gttattgaga tatataatga aaatttagat ataaaaatat
     6661 agatatagat ggtattaagt aatataatgg agaacttagg aatcagagag tagatagtta
     6721 gttacatatt atatttatgg aagaaattat ctaaaaatac tagaagtgat aaaaataaat
     6781 agaaattaat atctaaccta tagattcata taaaagtata agtgaattta taggtttttg
     6841 ttgtgtatag aaaaaaatac aattagattg tgtaatagtg tattaattta gcgatgaaat
     6901 tttagaggaa gaaatatgat taataacaaa tatagtatag aggaaaattt aaactatata
     6961 ctatcggggt gagaatttga aaaaaaatta taaaatacat aatctaaatg aatataaaaa
     7021 taaaaaaaag aataattata aaaagagaaa taagaaaaaa ataggagcta aatgcatttt
     7081 actgattgca attttttcag taataatatt gaatctatgt ggttattcaa aaatctcgga
     7141 aatgaagtac gatatctatt atttacaaaa agatttaaga aaaaaagaga tagtattaga
     7201 ggaactaaag gcaaaagaaa ctgaaaaaac atcaataaaa gaaattgagc taaaggctaa
     7261 ggaattgctc aatatggact atccaaaaga tggacaaata aagtatatag atgtggataa
     7321 ctagggggaa tagatttgag aaaagtaaag aggataagta agaaaaggct ggtactcgtt
     7381 cttattctag catgtgcatt gtttttttgc cttgttatta ggactgggta cctacaactt
     7441 atgaagggaa attggttgag tacaaaagca ctagaacaac aaacaagaga tataccaata
     7501 gaacccaaaa gaggaactat atatgataga aatatgaaag aattagcagt aagtgtaact
     7561 aagtatactg tttggtgtaa acctgtggaa gtagaagata aaaaagaagc agctgaaaag
     7621 gtagctgaga ttttggatga agattataaa gatatttacg ctttaataag caaaaaaaat
     7681 atggctcttg taaaagtaaa aagatggata gatgatgata aggcaagtca aataagagat
     7741 gctaaattaa gtggaatatg ggtagcagaa gataatcaaa gatattaccc atatgggaat
     7801 tttgcgccat atgtgttagg acatacttct tcagatgcaa caggtatatc tggtgtagaa
     7861 atgcaatatg ataaaaagtt aaaaggaaag cctggaaaac ttatagtaag tactgatgcc
     7921 tcaggaagag aaataccaca aggaatggaa aaatattatg agcctgtaca aggtaatggt
     7981 cttgtgttgt ctatagatga agtaattcaa cactatactg aaaaagcagt acagaaagct
     8041 tatgagttaa ataatgcaaa aaaggttact gcaatagcta tgaaccctaa aacaggtgat
     8101 atacttgctt tagcatcaaa accagattat gaccctaatg actcaagaac gccaatatat
     8161 ccatattatc aagaagaact tgaaaaatat aatgataaag ataaaataaa aggctattat
     8221 caaatgtgga gaaatccagc agtaagtgat acatatgaac caggttctac atttaagctt
     8281 attacatctt ccagtgctct tgaagaggga gtaattaaag atggagaaaa attcacatgt
     8341 acaggtagtg taactgttgg aggaagaaag attaaatgtt ggagacatta cagacctcat
     8401 ggaacacaag aatttaaaca agcagtacaa aactcttgca acccagtatt tgttgaatta
     8461 ggaagcagat taggtgttgg aaaaatgtat gattatatag aatcatttgg ccttatggat
     8521 aagacaggta tagatttacc aggagaagct aagggaatac tgtataacga aaaaaatgta
     8581 ggaccagttg aattggctac aatatcattt ggtcaatcta tatcagtaac tccaatacaa
     8641 cttataacag ctatatcatc aatagctaat ggtggagatt taatgcaacc aagagtggta
     8701 aaatcttata ctgacaataa aggaaatata actgaaacag taaaaccaaa aaaagtaaga
     8761 agtgttatat ctaaagaaac atctaaaaaa atgttagaaa ttgcagaatc agttgtaact
     8821 gaaggtggag gaaagatagc atatatacca gggtatagac ttggtggtaa aacaggtaca
     8881 gctcaaaaag ttatagatgg taagtatgct ccaggaaagt atatatgttc atttgttgga
     8941 atagcacctt gtgatgaccc gcaaatagta gttcttgcta ttgttgacga accaacagga
     9001 gtaagtgcat ttggtagtac tacagcagga ccaatagtga aagaaataat gaatgactct
     9061 ttaaaatatt tgggagttaa accagtatat aaagaagaag aaaaagctga gtatgaaaag
     9121 aagcaagtta aagtgccaga tgtaagaaat ctaaaaatag gagatgctgt aaaggcattg
     9181 gaggatgcga agttaaaacc agacttagat gcagatatag aacttccaga agatacaaag
     9241 gtaaaggata tattcccaaa acctggagta aaagttaatg aagactcaag tataacccta
     9301 tattttgaaa actaaaaagt aacagataat taacatatac ccataaacat cttcaaaaaa
     9361 acagaaatat agtatataat tataaagtta agagttatcg taggcaatgt atatataacg
     9421 gtatacattg ccttttgttg ctaagagaat atagctaagg ttttaaagga ggagtttaat
     9481 aatggagtgc ttaacaataa aagagttagt acttgcaact aatgggaagt taatatatgg
     9541 agattataat gactgtgtaa gtgatatagt tatagatagc agggaggcaa gtgcccaaaa
     9601 tgcatttgta gccatagttg gagaaaattt agatgggcat acatttatga agcctgcata
     9661 tgatagtgga tgtaaaactt ttataaaaaa tgaaagtaat ggaataaaat tagaaagttc
     9721 ggatataaat ttaatagagg taaaagatac atctttggct ctaggagata tttcaaaata
     9781 ctataaagaa aaatttgata ttccatttat aggtgttaca ggtagtgtgg gaaaaactac
     9841 tactagagat atgatttact ctgccatatc tgcaaagtta aatatactaa aaaatgaaaa
     9901 aaacttaaat aatcattttg gagttccatt gactttattt aatctaaata aagaacatga
     9961 gtgtgctgta atagagatgg gtatgtctgg attcaatgaa ataaaatatt tagtggatat
    10021 agtaaatcct aaaattgcag tcatatcgaa tataggatta tcacatgtag aaaaactggg
    10081 ttcacaagag ggtattttga aagcaaagat ggagattact tcaaactttg atgaaacaaa
    10141 tactttaatt gtaaatggag atgataagtt tttatctaca ttaaaagaaa aagaacatgt
    10201 atataagtta aaaacatttg gctttaataa aaacaatgat atttattgtg aaagttatac
    10261 tatggaagag gatagtttaa catttatatg tgtaataaat gggaaaaaag aggaaatatt
    10321 tataccaaca ataggagaac ataatatata taatgcaatg tctgctatat tagttggttt
    10381 gtgtctaaat atttctttag attatataaa aaaaggttta aaaaatttta aagggacaaa
    10441 aatgagacta gacataataa aaaatgaaaa attaacaata attaatgatt catataatgc
    10501 tagtccagat tctatggatg ctgctttaaa gatacttgga agatataaag gtagaagggt
    10561 agcgatactt ggagatatac tagaaatggg tgaaatttca gagtatggtc atagattagt
    10621 tggaaaatca tctatgaata atactgatat tataataact ataggtgaaa attcagtgtt
    10681 tattggagaa gaagcaaaac aattaggatt caattcagcc aatatatatc actttgagaa
    10741 tagagaagat gtgtttaaca agttaaatga attagtaaaa acaggagaca ctattttagt
    10801 aaaaggctca agaggcatga gattagaaaa aatagtcgaa tatctaaata aataagctta
    10861 aactaatgag ttaattgaca agggagagga gttttatgat gttaggaata acagagctta
    10921 cgtacaccgc attgattgcg tttttaatag taattataat aggaccaata tttataccaa
    10981 tgcttagaaa atttaaattt ggtcagacag ttagagatga tggaccccaa acacatctag
    11041 ctaaaaacgg aacacctact atgggtggaa ttataatgat tgttgcaatt ctaataactg
    11101 gacttacaag agtaaaagtt agtcatgata tggcagtagg tcttatatgt attgcaggtt
    11161 ttggttttat tggcttttta gatgatttta taaaaataaa gctaaaaaga tcacttggac
    11221 taaaagcata tcaaaaaata atccttcaag ttgcattatc tttttatgtt gctttttatc
    11281 agtatacatc atcatcaagt gcttcacaac ttatgatacc atttacagat tttgtgataa
    11341 atgttggaat attatatata ccaataatga tgtttataat cgtagcaata gtaaatgctg
    11401 taaacttgac tgatggatta gatggattag catcaggagt aacattaata gtttctgtat
    11461 tctttatgct atttgcaagt tctattgctg gaaatacaga agtcgcagtt ttagcagcag
    11521 caactgttgg agcatgttta ggtttcttag gatttaattc atatccggct agagtgttta
    11581 tgggcgacac aggttcaatg gcattaggag gtgcagttgt agcattttca gttctaacta
    11641 actcagtgct tataatacct ataattggag gaatttattt tgcggaggct ctatcagttt
    11701 tgattcaagt tggatatttt aaggcaacaa gaaagagatt ctttaagatg gctccaatac
    11761 accaccactt tgaacagtgt ggttggccag aaacaagagt tgtatttatt ttttggatta
    11821 taacagttgt tttagcttgg ataagtataa tagcagtatt ttagggctag ggggaatgaa
    11881 aaatggactt aaaaggaaaa aaagttttat tagtaggact tgcaaaaact ggtatatcta
    11941 caattaaaca tttagataaa ttgggtgcaa gtataatcgt aaatgatata aaagatgaaa
    12001 ataaattgag aaatatactt gatgaattga aatctataaa tgacataaaa tacatattag
    12061 gacatcaccc agaagatgta gatgatatag atatggttgt agtgtctcca ggtgtaccac
    12121 tagacctacc attcatattg aaattaaaaa attcaggcaa gtatataatt ggtgaggtag
    12181 aattagcttt taaactatcg aataatccaa tttttatagg gattacaggt acaaatggaa
    12241 agacaactac aactagtttg gtaggagaaa ttttttcaag agcaaaacga gatacatatg
    12301 tagttggtaa tataggaaat cctgtaatag atactataga aacttcaagt gaagagtctg
    12361 tgttagtaac tgaactaagc agttttcagc ttgagagtat agatgaattt agacctaaag
    12421 taagcgcaat attaaatatt acagaggacc acttaaatag acaccatact atggaaaaat
    12481 acatagaagc aaaggcaaat atatttatga accaaactgt tgaggatttc tgtgtgttaa
    12541 actatgatga tgaggtagta aaatctttag ctgataaatg caatgccaaa gtaatttact
    12601 tttcaagaac taaaaaagta aatggaggag tatatttaga aaataatgac ataataatag
    12661 atatagatga taaaatcaaa tttctaaata aagatgatgt aagtttacct ggaggtcata
    12721 acttagaaaa ttgtatggca gctatagcaa tagcatatgt ctgcaaaatt gatttagaag
    12781 ttataaagga tgtacttatg acatttaaag gagtggaaca cagacaagag tttgtaagaa
    12841 atttagacaa tgtaatatat gtaaatgatt caaagggaac taatccagat tccactataa
    12901 aagctataca gtcttatgat agacctataa tacttatagc aggggggatg gataagggaa
    12961 gtaattttga tgaacttctt gaaactgcaa agtcatatgt taaatcattg gtcttattag
    13021 gagaaactgc atcaaatata gaaaattgtg ctaaaaataa aggatttaat gacatacata
    13081 tagtaaaaga tatggaagaa gcagttaaga cttcttatga aatttcaaaa agtggagata
    13141 tagtgcttct ttcaccagct tgtgcaagtt gggatatgta tgaaagtttt gaagtaagag
    13201 gaaaagactt caaagataat gtaaacaatt taaaataatc tcttacctct aaacttactt
    13261 aaatggaaga atggaggtag attagttgga tgcctaaaga aaatttgaaa aaacaaattg
    13321 acataaggat gaagactgaa tttgatggag tagtgtttta cactactatg cttttagtat
    13381 ttgtaggaat agttatggtg tttagtgcaa gttttataca atcttcattt aaacacaatg
    13441 atgcgtacta ttttctaaaa aagaatgtaa tatatgctgt acttggattt atagttatga
    13501 ttattacatc aagaatagat tatagttttt ggaagaaaaa tgctacagca attggagcta
    13561 tagcagtagt attattattg ttagttttga cccctttagg gatagaagca aacggagcaa
    13621 aaagatggtt gggaatagga gctttaacat ttcaacctgc agaaatcgca aagtttgcta
    13681 caataatatt aactgcaaaa ttaattgaaa aaaattatga taaaataaaa tctttgacaa
    13741 aaggtgttgt accacttctc gtagttccag ggatattttt tgcgttgata atacttcagc
    13801 caaatttgtc aacagcaggt actgtaattc tagttacttt tgtaatgata tttgttgctg
    13861 gaatggatat gaaaattgta tttgctatga tagggagtgg ggctgcttta tttgctgcat
    13921 tagtaattgc tgaaccctat agacttagtc gtgttacatc atttttagat ccttttcaag
    13981 accctcttgg taaggggtat caggtaatac aaggtctata tgcactaggt tcaggtggat
    14041 tgtttggtct aggcttagga aagagtaaac aaaagtattt ttatatacca gagccacaaa
    14101 atgactttat atttgctata ataggagaag agttaggatt gattggttgt ataatagtta
    14161 ttatgctatt tgtagtttta gtctacagat gtgtaagaat agctttgaag acttctaatg
    14221 tatttgcttg tatggttgtt ataggtatag gtgcacaaat tggaatacaa gcagctttaa
    14281 atatagcagt tgcgacatca tcaatgccag ctacaggtgt agcactacca tttataagtt
    14341 atggaggaac ttcactcacc atctttatgg gagctgtagg aatagtgtta aacatctcaa
    14401 agcatgtcaa aattaattaa caggagatga tgatttgatg aaagttttat tatcaggcgg
    14461 tggaactgga gggcacgtgt atccagctat agctattgca aacaaaataa gagatgagca
    14521 tccagacgct gagataattt ttgttgggac ggaaaaggga atagagtcag aaatagttcc
    14581 taagtatgga tttgaattaa agactgtaac agttcaaggt tttaagcgaa aaatagattt
    14641 cgataatgta aaaagagttt ttaagctttt taaaggtttg gagcaatcaa gaaagattgt
    14701 taagaaattt aaaccagata tagttatagg aactggtgga tatgttagtg gacctgtttt
    14761 atttaatgca tctatgggga aaataccagc cataatacat gagcaaaatt cttttcctgg
    14821 tgtaactaat aagattttat ctaaaacagt tacaaaggta cttacaagtt ttgaagattc
    14881 gcataaaaga tttccagagg cagcagaaga taaattagtt tttacaggaa atccagttag
    14941 aaaagaaatt ttattatcaa gaaaaaatat tgcaagaaag aatctatcaa taagtgacga
    15001 aaaaagaatg gtactttgct atggtggtag tggaggttca agaaaaataa atgatgcaat
    15061 gagacttgta ataaagaata tggtaaatga agatatagct tttatatttg ctacaggcaa
    15121 aagctattat gatgaattta tgggaagtat tagcgatata aatcttaaac cataccaaaa
    15181 agttgtgcct tacttagaag atatggcaaa tgcacttgca gcaagtgatt tggttatagg
    15241 tagcgcagga gcaatttcat tagctgagat aacggctctt ggaaagccat ctataataat
    15301 acctaaagct tatactgctg aaaatcatca agagtataat gccaagagta tagaaaaaca
    15361 aggggcagga attgctattt tagaaaaaaa tctaactcca gagagcctaa atacagcagt
    15421 atttaaactt ttaggagata gagaactttt agttgatatg gcaaatgcaa gtaaaactat
    15481 aggtaagcct gaagccatag accttatata tgatgagata atgaaagtat acaattcaac
    15541 acaaaagtca acttctaaaa aaacaaaaaa ggaaaaagtt attaaggaag taaaagaaat
    15601 aaaaaaagaa acgactccaa gtatagaagg tcaagctaag gtaataggaa taaagaaaag
    15661 ataagtaa
//
LOCUS       DAEPOG010000094        15473 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEPOG010000094 DAEPOG010000000
VERSION     DAEPOG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.094"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            44..379
                     /locus_tag="KR432_002326"
     CDS             44..379
                     /locus_tag="KR432_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640856.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            516..1793
                     /locus_tag="KR432_002327"
     CDS             516..1793
                     /locus_tag="KR432_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBH0640857.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1914..3302
                     /locus_tag="KR432_002328"
     CDS             1914..3302
                     /locus_tag="KR432_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0640858.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3673..4386
                     /locus_tag="KR432_002329"
     CDS             3673..4386
                     /locus_tag="KR432_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBH0640859.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4504..5373
                     /locus_tag="KR432_002330"
     CDS             4504..5373
                     /locus_tag="KR432_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0640860.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5528..6673)
                     /locus_tag="KR432_002331"
     CDS             complement(5528..6673)
                     /locus_tag="KR432_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0640861.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(7006..7782)
                     /locus_tag="KR432_002332"
     CDS             complement(7006..7782)
                     /locus_tag="KR432_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0640862.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8364..9338
                     /locus_tag="KR432_002333"
     CDS             8364..9338
                     /locus_tag="KR432_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0640863.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9436..9741
                     /locus_tag="KR432_002334"
     CDS             9436..9741
                     /locus_tag="KR432_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0640864.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9897..11057
                     /locus_tag="KR432_002335"
     CDS             9897..11057
                     /locus_tag="KR432_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0640865.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11107..12009
                     /locus_tag="KR432_002336"
     CDS             11107..12009
                     /locus_tag="KR432_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0640866.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12089..12982
                     /locus_tag="KR432_002337"
     CDS             12089..12982
                     /locus_tag="KR432_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBH0640867.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      13035..13216
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13444..14574
                     /locus_tag="KR432_002338"
     CDS             13444..14574
                     /locus_tag="KR432_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640868.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14646..>15473
                     /locus_tag="KR432_002339"
     CDS             14646..>15473
                     /locus_tag="KR432_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0640869.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
BASE COUNT         5948 a         1718 c         2734 g         5073 t
ORIGIN      
        1 aaagtttgtt taataaatta aataaagaaa agaggagaat aaaatgtaca acatagatga
       61 tatgatatat aattgtccag tagaagcttt atcaaatatt ttaggtaaaa aatgggtggc
      121 tataattata tggaaaatac aagaagataa gaaacgattt ggagagctac aaagagctat
      181 accagactgt agtaagaaaa tgttagttca acaactagat tttttaattg aacaggggat
      241 tatagtcaac caaaaaaagt tggttaataa tgttgtagag tcaacatatt ttttaagtga
      301 atcaggatta cgattgcttc ctgtcatgga aaaaatgatt atgtggagca ataaaaattt
      361 aaattgtgat aaatactaaa atttactctg taagtaatga aaatttaatt aagctatgag
      421 tagaaaaatg tattaaaatt acataatttt ttagttagat tttaatttag aaatgggatt
      481 gtaatattgg aaataaaata aggaggaatt ttaatttgca aaatgtaaat ttatttttag
      541 tatttataga aggtatagta tcattctttt caccatgtat actgccaatt ttgcctattt
      601 atttgagtat attgtcaaat agtagtgtag aaaatctaaa agaaggaaag acgagtttta
      661 taggaagttc tttatttaaa aatactatat tttttgctct tggaatctca acaacattct
      721 ttatattggg ttcatcagta aaagtattga gtatgttttt taatgaaaat aaagatttaa
      781 taatgtttat tggtgggatt ataattatta tcatgggatt attttacatg ggaataatta
      841 aatcttcaat attgaataga gaaaaaagat ttaatgttaa gtttaaagaa atgaaagcaa
      901 taacagcttt tatattaggt tttacgttta gttttggatg gacaccttgt attggtccaa
      961 tattggcatc tgtattagtc atggtttcaa gttcaagtaa tcatcttagt gccaatcttt
     1021 taatagcagt atatactatt ggttttattt tgccatttat aattacagct atgttttaca
     1081 gtaaactatt taaaactata gataagataa aatctaacat ggaaattatt aaaaaaatag
     1141 gtgggattat acttatagta tcaggaatat taatgatggt aaatggattt ggaagtataa
     1201 gcaaacactt taatacgtca caaaatagca aaattgaaag caagcaagaa gaaaataagc
     1261 gagaaaattc tactgataaa gaagaaaatt ctgatggtaa taatagtcaa aaagactcta
     1321 ataatgacaa taatgataac gggtctaatg atgaagatag aataaagtct attgatttta
     1381 ctttaacaga ccagtatggt aaaacacata aattaagtga ttatgaaggc aaagttgtat
     1441 tcttaaattt ctgggctaca tggtgtcctc catgtaaaga agaaatgcct tatatagaac
     1501 aactgtataa agactataat aaaaacaatg atgatgtagt aattttagga gtagcttcac
     1561 ctaatttagg tagagaaggt tcaagggaac atgtagttaa cttcttaaaa gaccaaggtt
     1621 atacattccc tgttgtactt gatgaagatg gagcattagc atatcaatat ggaattaatg
     1681 cttttccaac tacatttata attgacaagg aaggatatgt aactcagtat attccaggag
     1741 ctatggataa ggcaactatg gcatcattta ttgaaaatca gagaaataaa taaaagtatt
     1801 taagtatatg tttatgaatc aatttaaggt tacaaaaaag tgcctaattg taccacctaa
     1861 ataatcttgt tatagtaaaa gtataaacaa tacaagaaag ggttgaattt tatatgaaaa
     1921 aaacatttga tgctataata ataggatttg gaaaaggtgg aaaaacatta gcaggagatt
     1981 tggcaaacag gggattaaaa gtagctttaa tagaaaagtc aaacaagatg tatggtggga
     2041 catgtgtaaa tgtggcatgt ataccaacaa aaagcttaga aaattcagca aacagcgtaa
     2101 aaacaaagaa tataaattct tgggatgaag tacaagctga atatgagaaa gctatagata
     2161 aaaaagaaac tttaattaca aaactcagag aagctaacta taataaatta aattcaaatg
     2221 agaatgtaac tatattcaca ggaatgggaa ctttcattga tgaaaaaaca gttcaagtta
     2281 aaactgaaaa tgaaatttat gagttagtag cagataatat atttattaat actggttcaa
     2341 gaccatttat acctaatatt aagggaattg aaaataagaa tatagtatat gatagtgagt
     2401 ctcttatgaa tctaagagtt ttacctaaaa aaatgacaat tataggagct ggatttatag
     2461 gtttagagtt tgctggtatt tatagttcat ttggagcaga agttacaata ttaaactcta
     2521 ataatggcat tttaccaaat gaagatgttg aggattcaga agaaataata aaattacttg
     2581 caaagagaaa tgtaaaaatt gttaataatg caaatataaa agagattaaa gaagtttctg
     2641 aatcagcaat agtagaatat gaagtagatg gtaaatctaa agaattaaca agtaatatga
     2701 tacttgtagc tacaggaaga aaagcaaaca ctgaagggct agggctagaa aatgcaggta
     2761 tagaattaaa tgaaagagga tttattaaag tctcagaaac tcttaagact aataaagagc
     2821 atatatgggc tataggagat ataaatggag gtccacaatt tacttatata tcattggatg
     2881 attatcgcat agttattaat cagttatttg gagataagac tagaacaact aatgatagaa
     2941 agaatatccc aaattctata tttataagtc cagcattttc aagagttggt cttaatgtta
     3001 aacaagctaa agaaaaggga tatgaagtat tagtggcaaa gatgccagta gaagcaattc
     3061 caagagctaa acagattggc aaggcagatg ggtttataaa aatagtgata gataaaaaat
     3121 ccaataagat acttggagct agtatgatat gtgaaaattc aagtgagatt atccatctta
     3181 ttcaattagc tgtagattta gaagtagagt atacatattt aagagaccgt gtttatgcac
     3241 atccaactat gacagaagct cttaatgata ttctatctcc taatatgata aaagaagtat
     3301 aacataagtt taatatataa acctaaaatt atcattataa gtagatttta gaaagaggct
     3361 atctcaaaaa atagagataa gtctcttttt tactttttaa atgataaaaa tatatttgat
     3421 tttataaaag aagattatct tatgaactaa aaagtttatt ttaaaacact acctatttat
     3481 gttataaact tataaatata atatagaatc tattattctg tattattaag tgaggttaag
     3541 cgttataatt aattataaat attgaataat ctaaatggat aagataatgg tattaaaaat
     3601 tatcttaaaa ggtgaaataa tctgtattta atgtagtaca gttatatctt taaagcaagg
     3661 tggtgaaatg atataaataa gatatctaaa agagggtttg atgatagtga taaaagatgg
     3721 ttttcaagta aagaattaac aagacttata aaggctaaag aagaaataga atggttatta
     3781 aatagggatt acaaaataga cccagtcgtt acttttgttg gtgatagata tcagttttct
     3841 ataagacaaa gagatgcatt aaaaagagca gtctgtactg atgaaaaaaa tattataaga
     3901 caatcaaaaa ggctttttct agataaaatc aaagaagata ccgtaaatat agatggcttt
     3961 aatttgataa tatccataga ggtagcatta tctggtggta ctctagtaat aggaagtgat
     4021 ggaaatataa gagatttagc aggtcttagg ggaacctata agattataga taaaacagaa
     4081 gaagctttga atctgatagg taaatttttt aataaataca atgcacagaa tcttaaattt
     4141 tatttagatg ctccagtttc taattcagga aatttaaagt ataggatatt agagcatgcc
     4201 aaaacttggg gaattgaaac tgaagtagaa ttagtaaaaa atgcagatgt tgtattggaa
     4261 aaattagata gagtagttag tagtgatgca gtcatagttg acaagtgtat aagttatttt
     4321 aatgtggcta gaggtataat tgaagagtat attaaagaat gtaatatcat aaatttaaat
     4381 aaatagataa atagaggtga gaatatataa gtatatgtag caatattatt agtaatacaa
     4441 gtaaagtttg aaaataggag aacgaataca tatcgaattt cataaaatgt tgtaaaaaga
     4501 catatgagag gatggatatg tgtgaaatta actaaactaa atattaaaaa atatagagca
     4561 cctatatata aatatgcttt agcaaatgta aatttgaggt ctgctaaatc tactaattca
     4621 agtatcataa cagtaatacc tcagggagct aaaatggaag tattggatga agaagatgac
     4681 tggattaagg tgatgtacaa ttctcaagaa ggatatgtgt ataaggattt ggtatcagta
     4741 agcgaatatg cttggagtaa cttaaactta agagaagata aatctataac ttccaatata
     4801 attacagtaa ttcctgaaaa atctagggtt gaagtccttc aagtagatgg agattggagt
     4861 aagattgtat atgatgataa aataggatat gtttttaatt attttttatc aactgatggg
     4921 aataaaccaa atgaattaga ttataagtat ttttatactg acatgattaa atttgttaac
     4981 gaaaataata ttaaaagtac tagcgattat ctaattgtaa cagaccttag aaataaatat
     5041 acttatattt ttaagaaaga taatggaggc tgggggcaac tttacaagtg gcaatgtact
     5101 ataggcaagc ctgagactcc aactataaca ggtatatttt atataagtgg aagaaaacct
     5161 tcttttggaa cggatgagta ttcagttaag tatgctacta gaattaaagg tggatattac
     5221 tatcattctg tgctatatga ttcaacagga agttatataa tagatggcag acttggtgaa
     5281 gctcttagtc atggatgtat aagattaagt acggaaaatg caaagtggat atatgataat
     5341 ataccagata caacaacagt tataattcat taagaaaaat tttgagtttt tattgggata
     5401 atgattctca aattaatagg attatatatt aacttcttca taaagagtac tatttaaaaa
     5461 gtgtaataag agagtgctat taacaaaaca aagatatttt gaggcactct cttataaata
     5521 tacataatta agatattttc ataaacaatt ctataatctt ctccttatca aagaaaacag
     5581 gattagcacc agcacaagca tctttcatag agttttctgc aagcaattca aaattaggat
     5641 tatcaacacc tacatctttt aaagattttg ggattccaac ctcttcagaa agagctttta
     5701 tcttatctat tacaaagtca acacactctt tatctgattt attatttaca tccattccaa
     5761 taacttcagc tattggtcgg aatttggcag gggctgattt agcattttcc tcctcaacta
     5821 ttggaagtag catggcatta catactccat gaggtaaatc atataaacct cctaattgat
     5881 gagccatagc atgtacattt ccaagacctg cattactaaa ggcaattcca tttaaaaaac
     5941 aagcataaca catttgttct ctagcttcta tgtcattacc atttttaaca gctcttggaa
     6001 ggtatttaaa tatctgcttt atagcatata aggcagttga atctgtaaca tcataagcac
     6061 cattagctac tacagcttct acagcatgag ttagtgcatc cataccagtt gctgctgtta
     6121 aattagatgg tttgctaatc attaattctg ggtcatttac tgtcattgta gctagtgagt
     6181 tggtatcaac cattatcatc tttacatgtc tgtcttcatc agttataaca tagtttattg
     6241 taacttcagc agatgtacca gcagtagtag ttatagctac gataggaaga cattttttag
     6301 aagttttgtt gataccttca tagtctctaa tatctcctcc attagtagct aatatagaga
     6361 tagctttacc acagtcttgt ggagaacctc ctcctatagt tataacgaag tcacaatttt
     6421 ctgattttaa tattttaagt cctttatcca cattagttac ggttggattt tgtttaactt
     6481 catcataaac aacaaagtct acatctatct gagataaaat atctgttact ttttttacag
     6541 taccatttga aactagaaat ttatcactaa caacaaatgc ttttttagtt cctaatgatt
     6601 taattgaatc tttagcttca ttaagacatc ctttcccaag taaatttatt ggtggtacat
     6661 aataagtgct cataactaaa atctcctttt attttcattg atttttatgt aaaaatatat
     6721 atctaacaat tttcactaat ttactcataa tatatcgtat cttagattga gatagggtta
     6781 ctggttctta tataagaact taattcgtaa ttacgattat gattagaatt taacatagta
     6841 tttttatcat gtcaataaaa atataaaaaa tattataaaa aaaacaaagt tactgtagtt
     6901 aaatcagcaa ctttgttttt atagtataaa tcaaataaat acattagttt aaaacaaaat
     6961 ttttaattat tatgttttga tatcttccat atacagaata ttaatctaat ttattacaat
     7021 gtcttcatta cgttcttcca tcattgattg aatatttttc tgagcattta agagtaattt
     7081 ttttatcagt tctatttttt cgtcattagc tctaaataaa ggcagtgtta cactaacagc
     7141 agctcttata catccatttt ttcttattgg tacggcaaga cattttaggt gttccattga
     7201 ttcttcacac tctgaagcta tttgtgttct ttgaacttca agaagctggt cgtataaaac
     7261 atcaaaatct gtaattgatt tttctgtaaa accttttaga ccatctggat ataagcttat
     7321 tagtttttcc ttgtcaaaat cacataaaag agcttttcca agacctgtac aattagcagg
     7381 tatttttttt ccaacataag aaataagtct aattggttct ggagaatcta cttttccaat
     7441 ataaagaact tcattattgt caagtatacc aagctgacaa gtttcagaac aggaattaac
     7501 tatattagtc atttcgtcaa ttatatagtt aaaaatagtt cttttttcaa aaaatgaaga
     7561 ccctaatgtg taagaacgta tccctataga atatttggat gtgtttttat caatagtaat
     7621 gtattttcta gcattaagtg tatgaactat aggaaagatg ctactttttg gagcattgat
     7681 aacatctgct atttctgtaa gagtatatcc attttcggat tctgctaatg cttctaaaat
     7741 atctaatatc ctagctgttg ggcgatgatt atctgaagtc ataaatttcc ccctcttttt
     7801 attaatttaa agtattagaa tatgttgatt ttagcacact atacgatatt ttacaatgta
     7861 gaaaataaat atgtaaattt atatataaat aagctcttga tttagataca gatagatatt
     7921 tttttagact gtacattaag gaataactaa tttccaaaat atacctaaag aaagtaagtg
     7981 atatttagtt atataattta agtaaatata tgcaatagtt atttttatat gtaaatatca
     8041 aaatattatg aatttatatt ttatctattt tttacactgt ataaaaactt tatagttttg
     8101 aatactatat aattttaaat acgctgtatt tttgcatata aggaagtcat ttgataatat
     8161 acaaggtatt atcagaaagt ttgaaaaaaa tttgacaaaa atttattttt tttattgact
     8221 cataaatagg aaaatgctat cattaattca aagattcgta attaaaaact tagttcttaa
     8281 atacgaaccg aaacggccgt tagaattaaa gctaaaaact taataagagt aattaaatat
     8341 catgttttag gaggattaac tttatgataa tggattttat taaaaaaatt cctgcaggta
     8401 tgatgatagt gccaatgttt atagcagctt tcattaatac cttttgccca gatattgtac
     8461 aaataggttc gtttacgaca gcagtatttt caagtgttgg gtctgcaact ataattgggg
     8521 ttcaactggt atgcttggga actcagcttc aatttagaga aatgccaaaa gttttaaaga
     8581 gaggtggtat acttcttggt tctaaatttg ctataggtgc tttgataggt atacttatag
     8641 gaaagctatt tggaatggat ggtgtccttg gtcttacaac attggctgtt ataagtgcag
     8701 ttacaaatag taatggtagt atatatcttt cattgatgac ttcatatgga gatgaaacag
     8761 attgtgcaac tatgagttta cttactttaa atgatggacc attttttaca ttaatagctc
     8821 ttgggacttc aggtcttgca aatgtgccat tgttgtcatt attagctgca atagtgccaa
     8881 tacttgttgg tatgataata ggaaattttg acaaaaaaat gaaagagttt ttagagccag
     8941 ggtgtaattt actcgtacct tttgtagggt ttgcacttgg tagtagcata aatcttgcaa
     9001 gtatactaaa aggaggttta tcaggagtat tacttggatt aatatctgta tttgtaggag
     9061 gtatatttat agtattctgt gataaattta taggaaaacg tccaggatat gcaggatggg
     9121 ctgtagctac aaccgctgga aatgcaattg ctgtacctgc tgctgttgca ttagttgacc
     9181 catcatggca accatatgtt gcaacagcta caacacaagt ggcagccgca gtagttgtca
     9241 ctgcaatatt agtacctctt ataactagtt ggtgggctaa gaagtatgga tgtcctaagt
     9301 tcccaaaaca aaatgaggat ggtacaataa cagcttaatt tatattacag tgagttgttg
     9361 gtaaaataat aaattcttta attagatttt aggatattaa taaaattata ttatttataa
     9421 aggagagcaa gaataatgat aaatgcagtg ataattgatg aaaaagataa tgttgcagta
     9481 gctattgaag caatagctaa aggtaatgaa attagtttta aattaaaaga caaaacagta
     9541 aaaacgatta cagcactaga taatataact atttatcata aattggctat aaaagatatg
     9601 gcccaaggtg ataaagttac taaatatggt gaacatatag gagaagcaaa tcaggaaata
     9661 aaaagtggtc aacatgtaca tattcataat gttagaagtg ttagagagga tttggataaa
     9721 caagttatga ctaatatata aaataatatt tatagagtta taaaagttgt attcattgct
     9781 tttagtgtca aatagttttt aaataggatt ttagctgttt gattagtgag tgtaataaaa
     9841 attataatat ataaaaatta taaaaagtat atagactatt ttaggaggaa atcagtatga
     9901 aattttatgg atataaaaga ccagatggaa gagttggtat aagaaaccac atattaatat
     9961 tgcctacgag tgtgtgtgca tcagatacaa ctagaataat agcatcacaa gttcaagggg
    10021 cagttacctt taataatcag aatggatgtt cacaagtaca ttcagaccaa caattaacta
    10081 tggatgttat ggctggttat gcagcaaatc caaatgtata tggtgtaata gttgtatctt
    10141 tgggatgcga aaattgccaa aatgatttgg tagttgatgc aataaaagag agaacaaata
    10201 agccaataaa aactcttgta atacaagaag aacatggaac tttaaaaact atagaaaaag
    10261 ctgttcgtta tgctagagaa atggcacaag aagcttcttt gcttagaaaa gaagaatttc
    10321 caatatctga acttatacta ggaactgaat gtggtgggtc tgacccaaca agtggattag
    10381 cagcaaatcc attaataggt gaattaagtg ataaacttgt cgatttagga gcaacatcaa
    10441 ttttaagtga aactactgaa tttataggtg cagagcatat actagctagg agagcagtaa
    10501 atgaagaagt aaaagaaaaa atattacata tagtacatag atatgaaaat tcattaaaat
    10561 tggttggaga agaagttaga gaaggtaatc catctcctgg aaatatagct ggtggtttaa
    10621 caagtttaga agaaaaatca cttggttgta tacataaagg aggtcataga caaataagtg
    10681 aagtatatga ttatgccaaa caaattgata agaaaggtct tgttataatg gatacccctg
    10741 gaaatgatgc ttcatctgtt gctggtatgg tagcaggagg agcacaaata gtggtattta
    10801 gtacaggaag aggaacacca tcaggaaatc caatagcacc agtaataaaa ataactggaa
    10861 ataaaataac ttttgcaaat atggaagata atatggattt tgatgctagt ccagttatct
    10921 atggtccaca aacaatggaa gaattaacag atgatttgtt aaatatggtt gtagatgtag
    10981 caaatggaaa acaatcaaaa gcagagtccc ttggatatac tgaaatggca atagctagag
    11041 tatgcaacta tgtataatct ttaagtaaga aaataaatac atagaatttt aataaagggt
    11101 gagattatga agtttgtaag ttttactgaa ggagaaaatg aatgtaaaag aactggagtt
    11161 ttttctaaag atgaattatt tattatagat gttaactctt taaatctcag tagaaatttt
    11221 aaggatttaa atgaattaat tcaaaatgta tctagtgaag atattattaa gttgaaaact
    11281 attataaatg aaagtatatt ctataattat gatgttttta aaagagagag atgtactgta
    11341 catgttccta tagaaaaacc aatacatgat atcttatgtg tgggagtgaa ctataaagac
    11401 catcttgaag aaactcaaac tcactttgat gatagttttg aagagccaca aaaaacggtt
    11461 tattttacaa aacgtgtgac aaaggcaata ggtccagaag atgagataga tggtcatttt
    11521 gagataaata atcaactaga ctatgaagtt gaacttggag tagtaatagg taaaacaggt
    11581 gttaatatta gaaaagaaga ggtagaagat tatatctttg gttacactgt tataaatgat
    11641 atttctgcta gaaagttgca aaaagaacat gttcaatggt ttagaggaaa aggattagat
    11701 acatttacct ctttaggtcc atgtatttta cataaaagtg cagttccttt tcccataaaa
    11761 cttaaagtat gtagtagggt aaatggtgaa gaaagacaaa gttctaatac aggattattc
    11821 atatcagatg tttcagacat agtttcagaa ctttcaaaag gtatgacttt agaggctgga
    11881 gacattatag ctactggaac accttctgga gtagggatgg gattttcacc tcctcgatat
    11941 atgagtagtg gagatgtagt tgaatgtgag attgagagta ttggaatttt aaaaaattac
    12001 attaagtaaa tgttttaata taattaatat taaagctaaa tctgatgtat taatgaacat
    12061 attaaaataa attttaggag tttaaattat gaaagcagaa tttttgacac cagttgttac
    12121 aatatttgat gaagaaggaa atttagataa agaaggtaat aaaaaagtgt acgaccatct
    12181 tatagatgga ggtgtagatg gactagtcat aatgggaagt actggagagt tctttaatat
    12241 gtctatggat atgcaaaaag aacttataga tttagtaact agtcatgtaa ataagagagt
    12301 aaaagtgttt ataggtacaa gtcgtatgtg tataaatgaa tctatagaac tttcaaacta
    12361 tgcacataat agtggagcag atggcgttat gataataagc ccttattatt tctctcttga
    12421 tgatgatagt attgagttat tttatagtga aatagctaag aatacagagg ctgaaatata
    12481 tttatacaat tatccagata gaactggtta tgatttaagt ccagaactta cattaagact
    12541 tgcaagaaaa cataaaaaca tagttggata taaggataca gtaactttga tgggacatac
    12601 cagagattta ataaatacta tgaaaataga gttcccagaa tttaaggttt atagtggtta
    12661 tgatgaaaac tttatacata atctaatgtc tgggggagca ggttgtatag ggggattatc
    12721 aaatttaatt ccagaagttt gctccaaatg ggctgaagct tacaataata gagatatgga
    12781 gaaggttata gaaattcaaa aatatattaa taaagcaatg gatttttact taataaccaa
    12841 tccatttata ccagcaatga aaaaagctat gaatatcaga gggttacaaa taagtgatta
    12901 tatgactgct ccatttgtaa aaccaaatga aaaacaagta tgtgaaatta aaaaattgat
    12961 gaataaatta aatatatgtt aaatcaacct tgcaaagagg tcaacaataa attacaatta
    13021 tcttaataaa aattaaatta aaataatact agggtactat attagtttaa tagggaaagt
    13081 gatgaaaatt cactacagcc cccgctactg tatacggata cgaaatcaaa atccactgaa
    13141 atttataaaa attttgggaa gggtgatgga ggaaaagccg tgagtcagga gacctgccca
    13201 gtattatata agttcaattt agggtggaga ttgtaattta taataaaatg aagtatcaat
    13261 tcgtttttgt aaagttgata tatgaacttc tccttattaa attaggagaa gttttttatt
    13321 ttattaaaag gttatactta ttaaagatga ctttaggata actttaataa gtatacttta
    13381 aaaagcatat attattaaag ttttttagcc actcataaaa aataagatat tggagtgaaa
    13441 gagatgaaat ttaaatcaaa tgttttaaag cagttttttt taattgctat ggtaattttt
    13501 atggtaggag gtcttatagg gtgtagtaaa agtgacaaag agaataaaca agctagtact
    13561 ggtaagaaat tagaattaaa atatgctagt ggatttagtg ttgaagattt agaagatgga
    13621 attaaaaaag taactgatgg agataatcga gaactgattt taatcccaaa gaagattaaa
    13681 attcctgaaa aatataaaaa tgctaatata gttagaacgc ctgtggacaa tgtattaata
    13741 gcatctacaa cacaggcttg ttctctgaga acaatagatg aactagatag tataaaagcc
    13801 gtaacaacag aagagcagta ctggactatt aaagaaatta aagatttaat gaaagatgga
    13861 aaaatacatt acgttggttc aaatttagca ccagattatg aaaaaatagt agacttaaat
    13921 cctgacttaa ctatagtgag tagtggttta tcagaggctg atacaaaatt tattgacaaa
    13981 ttaaaagaat tagggcttaa ttatgttgta gacaatgagt ataaagagca aaatccattt
    14041 ggaagaatgg agtggacaag tttaatagct gcattttatg ataaagaaga tatggctact
    14101 agtgaactaa ataaaactgt acaaaaggta gaagaagtaa gtaaaaaaat aaaaaacaaa
    14161 gctaaaccta aagttgtatg ggcatctgta tatgaaggaa gtgcttatat agctcctaaa
    14221 gattcatatg tggataatat gataaaaatg gctggtggaa taaatgcatg tgcttcaata
    14281 gattcaaatg aagggcgagt aagtattgaa caattgcatg aagttgcaaa agatgcagat
    14341 atatttgtat actcatcaag ttcagattat gcaccaaatt tagattctat aaaatctagt
    14401 gcacctgtat tggctgattt ggatgttgta aaaaacggaa acctatgggt gtttgctcca
    14461 gattattatc aatcagtaga taagacggac gaattaatag tagatttagt tgagatgttc
    14521 cacccaggta ctacaggtga aaaaatcaaa cattatataa attatgataa atagtacaga
    14581 gaggacacaa aaagataggt tttatagatt ttagttaata taactttgct aagaggagta
    14641 tacaaatgga ttttatcaat aaaatgagaa taaggaatac gacaatattt atagtattaa
    14701 ttataagtat gatacttact tttttagtgt gtgtagcatt tggctctgta aaaattgaat
    14761 ttaaagaagt aatagatgtt gtattaagag gtatggataa tacagaacat tataaaataa
    14821 tatacaatat aagaatccct agagtacttg caaccatggt aggtggagca tgcctagcag
    14881 tatcaggtat tttattacag atatttttta aaaacccaat agttgaaccg tatatattag
    14941 gagtatcttc aggttctact ttatcagtag ctctcatgat tttgggagga tacacattgg
    15001 gatttgagag tgtaaattca atgagtatat ttgtagcagc ttttatagga gctttaattg
    15061 taatgtctat agtcatttta tttgcaaata aggtaaagaa tataacaact cttttaataa
    15121 taggaattat gaccggatat gtatgtagtg gtataacaaa tgtgttacaa tcatttgcaa
    15181 atagtgataa attaaaagaa tttactatgt ggtctatggg aagtttttct ggatttacat
    15241 gggaaaaggt agaaatactt ataatagttg gtatattagg tcttttgatg actgtattca
    15301 taataaaacc tttaaatgct tttttattag gagaagagta tgcaaaaagt attggcataa
    15361 atataagatt ttttagagtt ttgatagtgt ttatatctag tatattagca gcaatagtat
    15421 cagcatttgc aggacctata gggtttattg gacttgcagt gccacaaatt tca
//
LOCUS       DAEPOG010000095        15444 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEPOG010000095 DAEPOG010000000
VERSION     DAEPOG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.095"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(45..779)
                     /locus_tag="KR432_002340"
     CDS             complement(45..779)
                     /locus_tag="KR432_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBH0640870.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            905..1621
                     /locus_tag="KR432_002341"
     CDS             905..1621
                     /locus_tag="KR432_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0640871.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1755..2447
                     /locus_tag="KR432_002342"
     CDS             1755..2447
                     /locus_tag="KR432_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640872.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2437..3618
                     /locus_tag="KR432_002343"
     CDS             2437..3618
                     /locus_tag="KR432_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0640873.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3900..4355
                     /locus_tag="KR432_002344"
     CDS             3900..4355
                     /locus_tag="KR432_002344"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH0640874.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4345..5070
                     /locus_tag="KR432_002345"
     CDS             4345..5070
                     /locus_tag="KR432_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH0640875.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5042..7423
                     /locus_tag="KR432_002346"
     CDS             5042..7423
                     /locus_tag="KR432_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH0640876.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7628..8857)
                     /locus_tag="KR432_002347"
     CDS             complement(7628..8857)
                     /locus_tag="KR432_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0640877.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            9457..10371
                     /locus_tag="KR432_002348"
     CDS             9457..10371
                     /locus_tag="KR432_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640878.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            10395..11030
                     /locus_tag="KR432_002349"
     CDS             10395..11030
                     /locus_tag="KR432_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH0640879.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            11406..12122
                     /gene="pyrF"
                     /locus_tag="KR432_002350"
     CDS             11406..12122
                     /gene="pyrF"
                     /locus_tag="KR432_002350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH0640880.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            12148..13191
                     /gene="carA"
                     /locus_tag="KR432_002351"
     CDS             12148..13191
                     /gene="carA"
                     /locus_tag="KR432_002351"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0640881.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            13244..>15444
                     /gene="carB"
                     /locus_tag="KR432_002352"
     CDS             13244..>15444
                     /gene="carB"
                     /locus_tag="KR432_002352"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0640882.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         6207 a         1713 c         2785 g         4739 t
ORIGIN      
        1 tataattaat acaatcttgc ttttttattt aaaaatttta attattattt aaaatttttt
       61 atcttctctt tatcccatat aaacaatttc tcagaattat ttaaagcgcc aaatacattt
      121 cttttaaaat caggcatctc tttatttaaa ttagcaataa gattttttat gtactctctc
      181 ctagtatgac catttgcagg gcagggattt gtgataatag gatagttaga atcttttgaa
      241 attttcttag tcatatactc ttctatgtaa atcataggtc ttataactgt taaatcctgt
      301 ctatctaggt atgtctttgg ggaaaaacaa tttactctac cctcataaaa catagacatt
      361 aaaaatgtct caattgcatc atctttatga tgaccaagag ctactttatt acatccaagt
      421 tttttagcat tatcgttcaa agcacctctt cttaaattag cacataatgc acaaggattt
      481 ttttcttgtc ttatatcaaa tacaatttct tttatgtcag tttgaaattc ataaaaagga
      541 acatttattt ctttacacaa tttatctagt ggagaattat caacacctcc agtatttaat
      601 gttatggcta tcaattcaaa gttttgtgga gaaaatcttt gataactttt aagtatatga
      661 agtagagtta aactatcttt tccaccagaa agtccaaccg caattttatc attttcttgt
      721 atcatatcaa aatcttgtat agccttacga gctttactta gaagtttttg tatagacatt
      781 ttatcaccat cctttaaaaa tattaaattt aagatttatt taagatttac ttataaaata
      841 cttaagtctt atactctaaa attaacatta tagacaaaca taacaaaaag gagaagattt
      901 aattatgaaa aaatttaaat taataggagc cattatttta gtagtattta taagtgtatt
      961 tatatctaaa gatagattac ttaagaaaga taaagatcca atagtagctg ctcaacatga
     1021 ttcagaacaa gatgttgagg caaattctca aaataaaaat cactcaaata gtatatcaaa
     1081 tatattattg gtaaacaaga caaatggaat tagcaaaaac tatactcctg aaaatattac
     1141 taaggtaaac ataccttttg ttgaagaagc aacagaggaa gaaaaacaaa tggcaggaga
     1201 acctgcaaag gcagttgaag atttagttaa gcaagctaat tcagaaggaa tacagttttt
     1261 agggtcttca gcatacagat cttatgatac tcaactggat acttatacaa gaagggtaaa
     1321 atctcaggga cgagaaaagg cagatgcata tgtagcaaag cctggttaca gtgagcacca
     1381 aactggactt tgtattgatt taacaaatcc agaaagatgg tttgttggaa gtacaaaaga
     1441 agctaaatgg ttggcagaaa atgctcacaa gtttggattt ataataagat atcctgaagg
     1501 taaagaagat ataacaggta cagcatatga accatggcat attagatatg ttggtaagga
     1561 tgctgccgag gaaatttatt caaaaggatt aactttagaa gaatatttac aaaataaata
     1621 atataaatgt gtaaataatt tgtattatat tggaaattta gaaatgaata aatttataga
     1681 ttacactaac taaaatatag tataatttta tatgaataaa gctatataca aagattaagt
     1741 taggatgtgt aaaaatgaat aatacagttc ttgttgtgga tgatgaaaag gaaataagag
     1801 accttataga gatatatctt ttaaatagtg gatataatgt aattaaagct gaagatggaa
     1861 aagaggctct agatatttta aaaaaggaaa atattcattt aatgatattg gatataatga
     1921 tgcctaaaat ggatgggata gaagtttgta gaaaagttag agaaagtttg aatataccaa
     1981 tacttatgtt aagtgcaaaa tcagaagata tggataaaat acaagggata atgactggtg
     2041 cagatgatta tttgacaaaa ccatttaacc cacttgaact tactgtgaga gtgaaatcac
     2101 tgattagaag agcatattat tttagtggtg ctaataatga agataaagat gtcataaagg
     2161 ttggagtagt aattatagat aaggaaaaac atagtgtaac agtggctgga aatgaaataa
     2221 ttttgacatc aagagaattt gatatattat atttactagc aaataataaa ggcagagtat
     2281 atagtacaga agaaatattt gaaaaggtat ggaaagagaa atattaccaa tctaataata
     2341 ctgtaatggt tcatatgagc agaataaggg ataaaattga aaaatataca gatggagaaa
     2401 aaataattca tactgtatgg ggagtaggat ataaaattga aaaataaaag tttattaaat
     2461 catatgtttc gacccatatt agatcttata ataagctgtg ctttaacttc aatgcttatt
     2521 ttaacaagta tgtatataat aaaattatta tatttcaata gtgagatatc tgtaattaat
     2581 caacttgtat atcagttaag agatttcaga agtcttatag gagagagttt attttatcca
     2641 tttttattta caatcacatc attagcaatt tattccatga tatcgtacaa gagaaataag
     2701 caaattgtag attttataag aaagactaat agaaaaaatc aaaaattaga aatcattaaa
     2761 agtgctgtaa atttaatgga tgaaggtaac ttagaaaaaa gtatagatat tgagtatgat
     2821 ttagatatat taaaggataa agaaaaggat gatattgatg aactagcaca taaaatcaat
     2881 aatatagtcc atcaacttag aaatataact attgaagaga gacaagctca gcagacaaaa
     2941 acagatttaa taacaaatgt gtcacatgac ttaagaacac ctttgacatc cataatgggt
     3001 tatttaggag tgatagaaga aggtaagtat aaagatgaag ttcagatgat gtattatgtt
     3061 gatattgcat atgaaaaatc gaaaaattta aatgttctta taaatgattt gttcgaattg
     3121 actaaaatgc aaaataatac aataaaacta aacaaaatag aaataaattt agtagagtta
     3181 ttaagtcaag tagtatctca atttgaaatt tattttaaac aagaatttat ggttagtaga
     3241 attaatttta atgaagaaaa attaattgtg aaagctgatc caaataaatt ggtaagagca
     3301 tttgaaaatc ttataactaa tgctgtgaaa tatggaaaag atgggcatta tgtagatata
     3361 gtcacagaga aaatggaaga tatggcggtt gtaaaagtga taaattatgg agaaccaatt
     3421 ccagtgttag atttaccaaa tatatttgat agattttata gagttgaaaa atcgagaaat
     3481 agaaatgttg gaggttctgg attgggtctt gcgattacaa aaaatattgt aaatttacat
     3541 gatggagaaa ttactgtttc aagcgataaa tatcaaactg tgtttgaggt acaactgcca
     3601 ataatacaat cagcttagtt ataaaataaa agtacgatta tgtgatataa atattataac
     3661 aataaagtgt ttacaaaaat aaaaaagtga tatataattt aagtcagttt aataagatac
     3721 ttatcaagaa ttaggggaga gagttagctc agtgatccta taccaacctt ttatcaggtg
     3781 gtcctgctaa caacgatgag atgacttgaa ataatatact cttcttatca ataatagata
     3841 ggaagagttt tttttagaat taatgtaaca aactttaaag tataatggag gattaaataa
     3901 tggaaaaagt agaaagtttt aagctagacc atacaaaagt aaaagctcct tttgtaagaa
     3961 agtgtagtgt acttgatgga gtaaagggag ataaagtaac taaatttgac ttgagatttt
     4021 tacagccaaa tgtagaaagt tttggaactg ctgctatgca cggtttagag catcttttag
     4081 ctacttattt aagagataca ttagatggtg taatagactt atctccaatg ggatgtagaa
     4141 caggatttta tttaatacta tggggagatg tagatgccaa aacagttaag attggtttag
     4201 aagaagcatt aaaaaaagtt cttgaatcag ataaaatgcc agcagctaca gccatagaat
     4261 gtggaaacta cagagactta tctttatttg gggcaaagga atatgcaaaa gatgttttag
     4321 ataaaggatt ttctttaaat atatatggag aataatttaa ttggaagaga agatataagc
     4381 ataaatcaaa gtgaagtttt aagatattta caatacagag gtcaagagat agataaagac
     4441 cttaaatata ctattgatga atgtgtagct ataactaaaa ataaaataaa tcctagatat
     4501 ttatctagga tttatcctat tagaataaat aaagataatg ttgtagaatt agagggaaca
     4561 aatttaacat tagagagtaa agatgtatat gaacttttaa aagaatgtaa tgagtgtgta
     4621 attatggcag ctactatagg gatagatata gaaaaagaaa taagacgaca ctcttattca
     4681 aatttaacaa aaggactaat aattgattca tgtggaacaa cagcaataga agaagtgtgt
     4741 gacattgttc aaaataattt agaaaaggtt tttttaaacc aagataaatt tataactatg
     4801 cgatatagcc caggatatgg agatttacca atagaaaaaa atatagatat attaaacgtt
     4861 ttgaatgctc aaaaacaaat aggtctaaca attacttcta atggaattat gataccgaga
     4921 aagtcagtag tggctattat aggtatttct aatacaagaa taaataaatc taagaaagaa
     4981 aagtcgtgtg aaaattgtaa aaactataga aactgtacat atagaaaagg agcgaactct
     5041 tgtggaaata agacaatatc tgaaaaataa tatattaata tttgatggtg caatgggaac
     5101 aatgcttcaa caaaaagggt taaagttagg tgaaaatcca gaagtatttg gtcttcaaaa
     5161 tccagataaa cttatagaaa tacatacagc atacttagag gcaggttcga atgtaattct
     5221 tacaaatact tttggatgta atgaactaaa attagatagt aagtatactg tagaagaggt
     5281 gattgacaat gctgttttgg tggctagaaa agctatagaa aatgtagata atactaaacc
     5341 aagatatgta gctttagata ttggtcctat aggagaaatg ctagagccta tgggtacttt
     5401 aagttttgat aatgcatatg agatatttaa aagacaagta ctacaaggcg ttaaatctgg
     5461 agttgatgta atagtaatag agactatgat ggatttatat gaagctaaag tagctgtatt
     5521 agcagcaaaa gaaaactctg atttacctat tttctgtact atgacatttg atgaaggtgg
     5581 aagaagtttt actggatgta tgccagaatg tatggtggct actatagaag gtctaggtgt
     5641 agatgcaata ggagttaatt gttcattagg acctaaacaa cttttaccaa tagttgaaaa
     5701 aatagcatca agagctacag tccctgttat ggttcaagca aatgcaggtc ttccaaatat
     5761 agttgatgga gaggctatat atgatgttga tgcaaaagaa ttttttgaag gggttaaaaa
     5821 gttcgttgaa gttggtgcta ctataatagg tggatgttgt ggtacgaatc caagttttat
     5881 aaaagaaata tctgagaata taaattctgt tacaaaggga tgtatcgaga aaattgataa
     5941 atgtgtagtc tgttcaccat caaaatttgt ggaagtacaa tcgcctacag ttgttggaga
     6001 aagattgaat cccacaggta gaaaatctct acaagaagca ttaaaaaatg aaaatgttga
     6061 ttatgctata aatttaggct tagaacaagt aaatgctgga gcacaaatac ttggagtaaa
     6121 tgttggtctt ccagagattg atgaaaaaaa actaatgcca aaattaataa gagagattca
     6181 agcagtagta gatacacctt tacaagttga ttcttccaat gtagaggcct tagagcaagg
     6241 acttagatat tataatggta gaacaatagt aaactctgta aatggcaaag aggagtcttt
     6301 agaaagtata ttaccaatag ttaaaaagta tggttcatgt gtggttggac ttacacttga
     6361 tgaaaagggg atacctaaaa aggctgaaga aagatttgaa atagcaaaaa ggatagtcaa
     6421 tagagcagtt tcttgtggga taaaaccaaa agatatattt atagactgtt tatcattaac
     6481 tgtttcagca caacaagaag aagcaataga aactataaaa gctataacaa tggtaaagac
     6541 actaggagta aaaactatat taggtgtttc aaatatttca tttggtcttc caaataggaa
     6601 agcacttaat gcttcttttc taacattagc tttaggggca gggcttgatt tagcaattat
     6661 aaatccaaat gaatatagta tgatggaagc tatcaattct tttaaaatat taaataacac
     6721 agataaagga tgtataaact atataaatca atatagtaat ataaataatt caaaaaaatc
     6781 ggattcatct acaaagattg acaaagattt gccattggat attttagttg aaagaggatt
     6841 aaaagatgaa gcaaaaaatg taacactgaa tcttttaaaa gagaaggatg aaaattacat
     6901 attagatgag attttaattc ctgctcttga taaagtagga aaaagatatg atagcggaga
     6961 tatattttta ccacaattga ttcaatcagc agaaacggta aaagtttcat taaatacaat
     7021 aaaggaaact ttattaagta aaagtagcaa taatgtatcc aaaggaaaaa taatagttgc
     7081 tacagtaaaa ggtgatatcc atgatattgg gaaaaatata gtaaaaataa tgctagaaaa
     7141 ttatggatat gaggttatag atttaggtaa agatgtccct attgaagaag tagtggaagt
     7201 tgctaaaaag agaaatataa aattagtagg attaagtgca cttatgacta cgactgttca
     7261 aagtatgaaa gatacaattc aggcactgag agataatgag ataaatgcaa aggtatttgt
     7321 aggaggtgca gttttgacag aagaatacgc tgaagaaatg ggcgctgatt attattcaaa
     7381 ggatgcaaag tctgctgttg aaatagcaaa gttaaatttt tagcatataa attaaaataa
     7441 aggttttact aaataggata ttttatatat aatataaata taagttttag atacttatgt
     7501 ttaaaacaat aggaattaat ctataataat gagattaatt aatataaaaa atatttatca
     7561 tcaacattaa aaagggctgg gaagtctggt ataacttcca ttagcccttt ttagttaaaa
     7621 tcatatgtta aaaagcccaa ttaccatttt taaaaatctg tatttgtttt ccatcataag
     7681 tctcaccaat aatatccata tcagcagaac caatcataaa atctacatgt gttaaactgt
     7741 catttacacc atgttcatct aattcttcat cttttaaatt gtttccacct tccagacaag
     7801 tcttataagc agaaccaatt gcaaagtggc aagaagcatt ttcatcatat agagtattat
     7861 aaaaaattat atccgtattg gatattggtg agttgtatgg aacaagagca acttctccta
     7921 aataatgaga gccttcatcc gtttcaataa gtttttctag tgtatctaat cccttttcag
     7981 cagagaaatc tattatttta ccatctttaa atgttaagaa gaaattgtca attatgtttc
     8041 caccataaac aagaggtttt gtactataaa cagtcccatt tactttaaac ttatgtggca
     8101 ttccaaaaat ctcttcagtt ggaatattcg gattaaaact tattccatta gggtctttgg
     8161 atgctccact tagccaaaca tgaccctttg gcaagtctag agtcaaatca gtttttgagg
     8221 atttatattt taaagtttta aagttatttt tatttagaaa gtccatttta gatttaagat
     8281 tttcattatg ttcttcccat gcttttatag ggtcctgacc atctactctt gaacatttaa
     8341 atatagcatc ccacagtttt tctattgctt tatcttctga taaatttgga aacacttttt
     8401 ttgcccaatc ttttgttggc acagagacta tactccattt acatttatca gttagagtgt
     8461 aagaacgcca ttcttttaaa gcttttccag atgccttctg aaaatttgca actctttcag
     8521 ggtcaacatc ttttaataaa tctggatttt gggcatagac tgataaaaat gccccacctt
     8581 cttttgctat atctacatat tgttctgctg tccatttggg gaactcatga aatgcttcat
     8641 ctggagcata caagtattta ataagagtac tttcttcatc agaccactct atataaacat
     8701 ttttagcacc acatttatat gcttctttaa gtgcatttct tgcaaaatca gcacattcta
     8761 ttggtgactt tataagtaga atttgacctg gctgtatgtt aactccaact tttattgcta
     8821 attcagcgta tttttctaaa tttctattta aattcatatt tatcctccta tatttttaat
     8881 attgaataac ttataaagta ttataacatt acttatgttt ttaatattga acaacttata
     8941 aaatattata actttactta gattttgggt catattcaaa atatttatac tttatagatt
     9001 gagaaataaa aaaaatattt taattaaaac agattatgag gataacaaac ttatttaagc
     9061 agtatgtttt ttgttgataa taatattaaa gtagaaataa aaatactttt ttagtgaaaa
     9121 gctatacaag aataccgaaa aaaacttata atataataat gagatagata tttataaatg
     9181 aaaaaaattt ttctggcaaa gcttaattaa aaaggtaata gctagtgttg ttatttaaat
     9241 tagcatagaa aatcaaataa aaaaattatg ctaatgtata gacattgata aggtagaatt
     9301 taagcctttt gaatgctata ttaaactaga gtagatattc ttatgaattt caaaataggg
     9361 caggaggaaa aaggaaataa aactataaaa aatataaagt atttatataa atttatagag
     9421 tttatacgtc agaagatttc agttaggagg gcataaatgg agctttatga agccattttt
     9481 tatagaaaat ctgtaagaaa tttttccagt aagaagataa aaaagccatt gatggaagaa
     9541 ataaaaagaa gttgtaaaaa tttagaatac ttaaatgaag atttaaatat taaagcacat
     9601 gttgttgata gaggtcatat aatacatttt ttgatgggta aggaatgtaa agtaaaggca
     9661 cctcattata tagtagttac atcagacaaa ggtgaggact atttacagaa tataggattt
     9721 gctacagaag gtgtagtttt gcgccttaca acattgggaa tagctacttg ctggttggaa
     9781 ggaaacttaa agagagaaga tatcttggaa tttgtagata ttgggaataa gaattttgaa
     9841 gaagatgagg aagatgattt agattatcta gctggagtaa aaacagaaga agatgatgaa
     9901 aacaaaatag aaaatcctta tataataata gcatttggat atccagcaaa aaatgaagag
     9961 ttgtttagga cgagatatgc tagacctgat agaaagccag taaaagatat gataaataag
    10021 ataaatagga atagaaagtg gaaagatatc ttagaattga caagaagagc tccatctgta
    10081 aaaaattctc aaccgtggta tttccataag gatgaacgag gtcttcattt atttgaaaag
    10141 agacctaaga aacactgtga agatatgaat aaggttagct taggtgtagc cttaagacat
    10201 tttgatatag cttgtataaa aaataaaata gatgtttcat atgaaaaact tcctataagg
    10261 aataaaatag gaaaatcata ttttataact gtagtcgagc atgtgaaacc agaagaagaa
    10321 acccaagaag aaaatgtaac tctagaaaaa gaagaatcac aagatgaata aaattttgga
    10381 aggggatatt ggatatgtat aagtgtggaa aatgtaatct tagaggctgt tctaatggaa
    10441 atatggataa attaatgaag atatgtccaa ctagagaaat tgaactacaa caaaaagcaa
    10501 agcaacttta tgaagaagaa gagaatagaa aaatagctta taatgctgga ttagttgaag
    10561 cagaggggta ttgtcaatat actagactaa aagagattat agagtttgct aaaaagtgtg
    10621 gttataaaaa gattggcata gcattttgta ttggactaaa aagagaaatg caaacagcac
    10681 aaaaaatatt tgaacatcat gggtttgaag tattttctgt tatatgtaaa aatggggcaa
    10741 tgcctaagag tacaattgga ctagaagcat ctcaaacaat atcaaagtgt tcagatgagg
    10801 taatgtgcaa tccaatagga caagctttat ttttaaatga agctaaggtt gaccttaatg
    10861 taattcttgg attatgtgtt ggtcacgata ctttagccat gaaatatatg gaagctccta
    10921 taacaacttt agttgtcaaa gatagagtaa ctgggcataa tccagttgct gcaatatata
    10981 atgcagaatc atattttgag aaaaaattgt ttgataaaga tgaagaataa ttcaaagact
    11041 tattgacata aaaagcaatt agtggtttaa ttataaaaaa gataaaactt taaattatcc
    11101 tatagataca taaagttata taaataaaga aaaatattac agctaaattc cttaaagtag
    11161 tccagagaga ctaaagggtt atagataatg ttagtatata acctctagta aatcttctag
    11221 aggtttttga tgagtataaa ataaaaattt aaatataata aagtctttta aactagtcca
    11281 gagagactgg gaaagatatc tcatagctaa tgaatggagt tatgtataga taaaatcctt
    11341 atgcctcttt tggtgtgagg atttttttat gattatttag gctttattaa aaaggagatg
    11401 taaaaatgat aaatggtaaa gaaaaattga tagttgcaat tgatactgac gaatttgata
    11461 aagctaaaga actaatagac agattagaag atagtgtaga catatttaag gtaggtctag
    11521 agcaatacgt tgccacaaaa ggtaaaacaa tagattatct taaagaaaaa gggaaaaaaa
    11581 tatttttaga ccttaaattc catgatatac caaatactat gaaaagtgca gtgagagctg
    11641 cagtgagaga taatgtttgg ttaatgacaa tacatgtatc agatatggaa ggaatgagac
    11701 aatgtgcact aattgctagg gaagaagcag aaagattaaa tatagaaaag ccattagtag
    11761 ttggagtaac agttttaact tcattaagta accaagattt acaggacata ggatgtaata
    11821 tgacaacaga agagttagct ataaagagag ctaaactggc aaaagaatca ggcatagatg
    11881 gggttgtttg ttcagcgcaa gaagtagata aaatagtaga agcatgtgga gctgatttta
    11941 taacagtttg cccaggaata agacctaaat cagcagaggt tggagaccaa aaaagagttg
    12001 taactccatc agatgcaata aaaaaaggtg ctcattattt agttgttgga agacctataa
    12061 caaaagcaga aaatccaaga gaatcagcta ttaatattgt aagagaaata gaaaatgctt
    12121 agataaaata tttgggaggt agcttatatg aaggggaaat taattttaga agatggaaca
    12181 gtatttgaag gaaaagcatt tggatatcta aaggaagcta ttggagaagt tgtatttaat
    12241 acttcaatgg ctgggtatgg agaaatattg acagacgcaa cttattatgg acaaatagtt
    12301 actatgactt atccacttat aggaaactat ggaataaatt tagaagatgt tgaatcaaat
    12361 aaggtgcaag taaaaggact tattgtaaga gaaaaaagtg ataacccaaa taattttaga
    12421 tgtgaaatgg atatagacac atatctaaag caaaataagg taataggact tgaaggaata
    12481 gatacaagag cattaactaa aattattaga aacaatggaa ctaagagagg aattataact
    12541 ttagaaaatg tatcattaca agatgttcag tctaaattag aagagcattg taacaaagat
    12601 tcagtaaaaa atgttacaag aaaagaaatt gaaaatataa aaggtactgg agctaaagtt
    12661 gtagttatag actttggtgt taaaaatagt gtactagagt ctcttaaagc ttgtaactgt
    12721 gatataacta tagcaccagc aatgactaca gtagaagaaa ttttaaatat aaatcctgac
    12781 ttaatcttat tatctaatgg accaggaaat ccagaggata taaatgatat agttgaaaat
    12841 ataaaaaaat taataggcaa aaagccattg acaggaatag gtttaggaca tcaaatttta
    12901 gcattggcat taggaggaaa gacttcaaag cttgtatttg gacatagagg aggtaatcac
    12961 ccagttaaat gtttagaaac tggaaaggta tttataactt ctcaaaatca tggatataat
    13021 gtttcagaaa tgccagaaaa tacagaggta actcacataa atttaaatga caatactata
    13081 gaaggaatga gacataagga attaccaata tatagtgttc agtacattcc agaatacaca
    13141 acaaaagaag atttagattg tatatttaat aaattcttag cattaatata gatttagtat
    13201 tatttacaga tagtaaaaac catttagata aggagaaaaa tttatgccta aattagatag
    13261 tataaagaaa actttagtat tagggtcagg accaataata ataggtcaag ctgcagagtt
    13321 tgactattca ggaacacaag cttgccagtc tttgaaagag gaagggattg aagttgtatt
    13381 aataaatagt aatccagcca ctataatgac tgataaagaa gtggcagata aaatatatat
    13441 agaaccatta acaatagaat ttatagaaaa aataatagaa aaagaaagac cagacagttt
    13501 acttgcaggt atgggtggac aaacagggct aaacctagca gttgaacttt atgaaaaagg
    13561 catactagat aaatatggag taaagataat aggtacttca gtagaatcta tcaaaaaagg
    13621 tgaagataga gatatattta gagaagtaat gaaagaaata aatcagcctg taatagttag
    13681 tgatatagtt acagatttac aagcaggtct tgattatgca cttacaattg gttatccagt
    13741 agttgtaaga ccagcataca cactaggtgg aactggtggt ggtatagctg ataatgagga
    13801 agaattaaga gaaatacttt cacatggtct acaattaagt cctgttggac aagtattaat
    13861 tgaaaagagt ataaaaggtt ggaaagaaat agaatatgag gtaataagag attcaaaggg
    13921 aaattgtata gttgtatgta acatggaaaa tatagaccca gtcggagttc atacaggaga
    13981 cagtatagtt gttgctccaa ctcaaacttt aagtaacaaa gaatgtgcaa tgcttaaaaa
    14041 agcatcttta gatatattaa atgcagtaga agttcaaggt ggttgtaacg ttcaatttgc
    14101 acttaatcca catagttttg aatatgcagt tatagagata aatccaagag ttagtagatc
    14161 atcagcttta gcatcaaaag ctacaggata tccaatagct aaagtttcat caaaaatagc
    14221 tttaggttat actttagatg agatagaaaa tgcagtaact aaaaaaactt atgcttgttt
    14281 tgaaccaact atagattatg tggtggcaaa aataccaaaa tggccttttg ataaatttaa
    14341 aaaagcaaat agaaaattag gaactaagat gatggcaact ggtgaaatta tgagtatagg
    14401 aagtaacttt gaggctgcta ttcttaaagg gataagatca cttgaaacag gaaaatattc
    14461 attagttcac acaccatctg aggagagaag cattgaggaa ttaaagaaaa gagttgtagt
    14521 acctgatgat gagagactat ttgacttagc tgaaatgata agaagaggct ataaagtaga
    14581 gatgatagaa caaattacag gtatagataa atggtttata aataaattta aatggatagt
    14641 agagcaagaa gaaaaattaa aagttatgaa aattgaagat ttaactaaag actatcttct
    14701 tgaattaaag aaaaagggat tctctgataa aggtatatct gacttaatga agataagtcc
    14761 agaaaaatta tatgaattga gaagtctata caatatcaaa ccagcatata aaatggttga
    14821 tacttgtgca ggtgagatag atgctatatc tccttactat tattcaactt atgagcaata
    14881 tgatgaggta gttgttagtg ataagaaaaa ggtgatagta ttaggttctg gtccaataag
    14941 aataggtcaa ggtatagaat ttgactattg ttcagttcat tgtgtaaaat ctttaagaaa
    15001 aatggacata gaaactataa tcataaacaa taaccctgaa actgtaagta cagactttga
    15061 tacatcagat aagctgtatt ttgaaccatt aacagaagaa gaagttttaa gtataataga
    15121 aaaagaaaag ccagaaggtg taatactaca gtttggtggg caaacagcta taaaattggc
    15181 taagttttta catgaaaaaa atatacctat actgggaaca gattttgatg atatagatgc
    15241 agcagaagat agagaaaaat ttgatgattt actagagaga ctagatataa atagaccaaa
    15301 aggaaagggt gtatggtcac ttaacgaagg tattgaagtt gctaatgaat taggttatcc
    15361 agtattagtt agaccatctt atgtacttgg tggtcaaggt atggaaataa cttataacga
    15421 agaaaaatta tctcaatatc taca
//
LOCUS       DAEPOG010000096        15096 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEPOG010000096 DAEPOG010000000
VERSION     DAEPOG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.096"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..371
                     /locus_tag="KR432_002353"
     CDS             <1..371
                     /locus_tag="KR432_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640883.1"
                     /translation="EINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTK
                     FFKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGV
                     SPLEFKKRYNSLEKKGGASK"
     gene            776..2224
                     /locus_tag="KR432_002354"
     CDS             776..2224
                     /locus_tag="KR432_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0640884.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            2250..4640
                     /locus_tag="KR432_002355"
     CDS             2250..4640
                     /locus_tag="KR432_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0640885.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4642..5313
                     /gene="pgmB"
                     /locus_tag="KR432_002356"
     CDS             4642..5313
                     /gene="pgmB"
                     /locus_tag="KR432_002356"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0640886.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            5352..6752
                     /locus_tag="KR432_002357"
     CDS             5352..6752
                     /locus_tag="KR432_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0640887.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            7069..7911
                     /locus_tag="KR432_002358"
     CDS             7069..7911
                     /locus_tag="KR432_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0640888.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            8056..9984
                     /locus_tag="KR432_002359"
     CDS             8056..9984
                     /locus_tag="KR432_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0640889.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            10045..11472
                     /gene="ascB"
                     /locus_tag="KR432_002360"
     CDS             10045..11472
                     /gene="ascB"
                     /locus_tag="KR432_002360"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0640890.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11883..12659
                     /locus_tag="KR432_002361"
     CDS             11883..12659
                     /locus_tag="KR432_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0640891.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12675..13040
                     /locus_tag="KR432_002362"
     CDS             12675..13040
                     /locus_tag="KR432_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0640892.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(13206..13955)
                     /locus_tag="KR432_002363"
     CDS             complement(13206..13955)
                     /locus_tag="KR432_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0640893.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(14400..14957)
                     /locus_tag="KR432_002364"
     CDS             complement(14400..14957)
                     /locus_tag="KR432_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0640894.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
BASE COUNT         5912 a         1653 c         2595 g         4936 t
ORIGIN      
        1 atgaaataaa ctctaaagag atattagaga gattgaacaa tatattcaaa tatgtagaaa
       61 aaaattataa acaacctata aaattagaag atgtgtctta tgaagtagga tttagtgtat
      121 attattttac taaatttttc aaaaaaaata caggaaaaac atttataact tttttaaatg
      181 aatatagatt agaaaaagca aagtggatat tacttaataa caatatatcc attgtagatt
      241 tggttgaaga agttggtttt ggaagtacaa aaacttttta cagagtattt aaagaaaaaa
      301 ttggagtctc tccattagaa tttaaaaaaa gatataatag cttggagaaa aaagggggtg
      361 cctcaaaatg aacttagttc atgaggcact ttcttttata tgaaatcaaa tatattttca
      421 gtatttcaac aatgaaaatt ccattcattt ataaagtcta aattttagtt ctcaatacaa
      481 tctctatttc gaaataaatc cttttatata tataaatata cagcaatata tgagaataag
      541 ataacaataa tgaggatata aaaatgtatt tgctatatat ataattatat acatagatat
      601 ctaatattga tatttaatat gaaagtattg aaaatagaca atattaatta tagtgagtaa
      661 aaaatataat tggattttta aattgaatta tttaaaaggt atagtatgaa aaaaatctta
      721 taactaaata tgagatagaa attataaaac tttatactgt gaaagaggta gatgcatgaa
      781 attagggaaa gattttttat ggggaggggc aatttcagcc aatcaaagtg aaggatttta
      841 tttagatgga ggaaaaggtt tatctcagtt tgataaactc ccattaaatg ataagagatt
      901 aaaagatgta tgtttagatg aggataatat tttaaatgaa aataatcaat actatccaag
      961 tcatataggg attaaatttt gtgaaaatta caaagaagat atagcaatgt tagcagaaat
     1021 aggttttaaa tgttttagat tttctatatc ttggtcaaga atattcccaa caggagaaga
     1081 aagtgaacca aatgaaaagg gattacaact ttatgataat ataataaaag aattaaaaaa
     1141 atataatata gagccattaa taacgataag ccactttgat ataccttatt ctctagttga
     1201 aaaatattct gggtgggctg ataggagagt ggttaattta tatgtaaaat atgctgaaat
     1261 tttgatgaaa agatataaag atgatgtcaa atactggatt ccttttaacg aaatgaatat
     1321 gataattcat attccatata taggtgctgg gttaactttt atagctgatg aaaatcaact
     1381 tcagaaaaaa tatcaagcag cacaccatca attattagct aatgcaaaaa ctatagaaat
     1441 aggaaggtat ataaataaag aatttaagtt tgggtgtatg ttagctgctg gaaaaacata
     1501 tccatacaca tgtaaaccag aagatgtatt tgcaacttta gagcatgaaa ggcataattt
     1561 gtttttttct gatgttcaag ttaggggtgt gtatccaagc tttatgaatt attatttaaa
     1621 atcaaatcaa ataaaattaa taataaaaga ggaagattta tcaacactta aggataatac
     1681 tgtagatttt gtatctttta gctattattc tagtgcttgt agttcagcta gagctgaagg
     1741 tttagaaaaa tcaaaggcaa acggaccaga aaccataaaa aatccatatc ttccaaaatc
     1801 aaatagtgta tggcaaaacg accctgtagg gttaagaata actatgaatc agttatatga
     1861 aaggtatcag ttacctttat ttatagtaga aaatggactg ggtactcaag atattgtaaa
     1921 tgaagatgga aaaataattg atgattatcg tatagattac atgaaagaac atattaaaaa
     1981 tatgattctt ggagtaaatg aagatggaat agagcttata ggatatctta tgtggggatg
     2041 tatagatgta accagtgtaa gtgaaggaaa gatgacaaaa cgatatggca taattcatgt
     2101 tgattctgat gactatggaa gaggctcatt taaaagaact aaaaaacaaa gtgctttttg
     2161 gtataaaaaa gtaatagaaa cagatggaga aattttattt aatgatatgg aaactcatgc
     2221 ataattcatt ttaagaaagg agtgttatta tgagtttaat atttactact aaggaccaac
     2281 ttatagagca agggtttagt aaggatacaa taaaatccaa tgaaacgttg tttacactag
     2341 ctaatggaca tttaggagtt agagggaata ttgaagagtc cgattttaat aatgagtatg
     2401 tagataatat gggtacatat gtaaatggat tttatgaaga atctccaatt acatatggag
     2461 aaagcgctta tggctatgca aaacaaaatc agactatctg taagttacct aatgctcata
     2521 tagtaaattt ctctattgaa ggagattgct ttgatttatc aaaaggtatt acaagtgaac
     2581 atactaggat actagattta aatgaaggaa ttgttaaacg ttcatttata tgggaaaact
     2641 ctcgtggtag aaaagttaaa gtatctatag aaagattagt atcacaagat atagaagaat
     2701 taatgatgat ttcatataca ataacaccat tgaattttaa tggaaaaata aaatttaaaa
     2761 ataaaatgga aaatagtatg gagaagttta attgtgaaaa agtagatgac ccaagagtgg
     2821 catctataaa taagaagcaa tttaaaatta atgtaaaaaa atataacaat aagtatttta
     2881 tgaatttagt tacagaaaga agtagattag ggttatggtg tgggtttgat tcatatatta
     2941 cttgtggaaa tattacaaat gagacctata atgtttcaga tgaatgtatt gagcgagaaa
     3001 tagaagtcta tgccaaaaaa gatgaaaggt taaatttaga aattgttgta ggctatagta
     3061 gagtatattc tgatttaaat ctaaatgaag aacgtttaaa atcattagaa tgtctggagt
     3121 cgttaaaatg tttagaatca gaagcacatt taaaatcatt agaattggtt ttaagagaag
     3181 catgtaatat tggatttgat aaagtcaaaa atattcagag aaagaaaatg aatgagtttt
     3241 ggacaacctc tgatgtagag attgaaggcg ataaaactct tcaactgggt ataaaaacca
     3301 atttatttca tatttatcaa tcagcaggaa gagatggaaa gactaatata tcagcaaaag
     3361 gtcttacagg tgatggctat gaggggcatt atttctggga tacagaaatg tatatattac
     3421 cattttttat atatactcaa ccatccattg ctagagcttt attagattat agatactcaa
     3481 tcttagatgc atcaagagaa agagctaggg aacttggaat aaataaaggt tgcttatatt
     3541 catggagaac tataaatggt aatgaatgca gtgcatatta tccagcagga acagcacagt
     3601 ttcatataaa tgcagatata gcttatggta ttagaacgta ttttgaagca actaatgatg
     3661 aagaatttat gatgcaaaat ggcttagaaa ttcttataga aacagcaaga ttttgggttg
     3721 aatttggaga ttatatagaa ttaaaaaata ataaattctg tataaatgga gtaacaggac
     3781 cagatgaata tacagcaata gtaaacaata attactacac taatttaatg gctaaatata
     3841 atttaatata tgcagtacag atggtagata aatttaagca taatttattt tcaaatgaag
     3901 tatttagaaa agttaaatta caagaatatg aagtaaaaga ctggataaat gccgctcaaa
     3961 atatttattt accatatgat gaagagaaga aaataagcaa acaagacgat tcatttttag
     4021 aaaaatcagt atgggatttt aaaaatacac caaaagaaaa atatccatta cttctcaatt
     4081 atcatccatt aacaatatat agatatcaag taaataaaca agcagatact gtactttcag
     4141 cgttgctttt ccctgatgag tttagcttag aacaaaagaa gagagatttt gaatactatg
     4201 aaaaaataac tactcatgat tcatctttgt caaggtcaat atttggaatg atggcaagtg
     4261 aaattggaaa tcatgaaaaa gcttataact attttatgga cacagcatta atggatatta
     4321 ataacctaca aaaaaataca agtgatggaa tacatgctgc taatatgggt ggaacatgga
     4381 tgagtttagt atatggattt gcaggaatga aaataaaaaa ggataaatta agttttgaac
     4441 caaggatacc taaacactgg aaaaatatta aatttaatat attatttaga gataatttag
     4501 tttctgtaaa tatagaagaa tataaatctt attataagtt aatcaaaggt aaaaacataa
     4561 gcatattaca tgataataaa gaaataaaat tatcaaagaa cataatagaa gttaataatt
     4621 gttttaaagg gagtaattaa tatgaaagca gtcttatttg atcttgatgg agttataaca
     4681 gatactgcta agtatcattt tgaagcttgg catgagatag caaaatcgct agatatagaa
     4741 atagatgaaa cttttaatga aaaattaaaa ggcataagca gggaagaatc attagataaa
     4801 atattgttat ctgctaataa agtagatagt tatactaaag aggaaaagtt aaaggtgtgt
     4861 gaagaaaaaa ataacattta tataaagtta ataaactcat taacatataa agatatatta
     4921 ccagggataa atgagcttat aaaagaactt aaaaaaaata atgtgaaatt gggaatagca
     4981 tctgctagta agaatgcacc taggatatta gaatctttgg gaataaaaga tgaatttgat
     5041 tgtatagtaa atccatcctt attagaaagg ggaaaaccat atcctgatat ttttattgaa
     5101 gggagcaaga tgctaaattt aaaacaatat gattgtgtag gaatagaaga ttcttattca
     5161 ggtattaaag ctataaatgc tgccaatatg acatcaattg gtgtggggaa aaaagaagta
     5221 ctaggaaaat gtgaccatat tgtagaagat actagcttat taaattacaa tacactatta
     5281 caagcttgga gtaaaaagat taatttggat taaaattata ttaaaaatta ttttaaaaga
     5341 gaggataagg tatgaagaat aaatatgaag aagtagctca attgataatt gatggacttg
     5401 gaggaaagga taatataatt gacttaactc attgtatgac acgattaagg ttcatcttaa
     5461 aagatgaaaa gaaagtcaat gcagataaat taaaatctat agataaggta gttggagtaa
     5521 actcaacttc aactcaatat caggtcataa taggtaatga ggtaggtgtt gtatttaatg
     5581 caataataaa taaggggata aataccacag gagatattaa aagcgatata aaaaatgaaa
     5641 ataaaaaaaa tagtatttta aataatataa tagatacaat aactggttgt atgacaccta
     5701 tgataccagc ccttacagca tctgggatga taaaagttat attatctctt tgtaccactt
     5761 ttaattggct atcatcaaat tcaagtacat atagagtatt agatataata ggagatgtag
     5821 cattttactt tatgcctata atacttgcaa taaatgcagc taaaaaattt aatgttaata
     5881 catcaatagc agttatagta gtaggtgtgt ttttacatcc aaatttctct gcatgggtat
     5941 caagtgggga ccctatatca tttataggag taccaattca aggtgttata tatgcagcat
     6001 ctgttatccc tgcactttta acagtatgga tgatgtcata tatagaaaaa ttcattaata
     6061 aattaacacc tagcatgtta aaaacaattt taaatccaac tttagtatta ttaataagtg
     6121 cgccaatagc attaatagta attggaccaa taggaaattt cttaggagag ggattagcta
     6181 gtataataaa tttactacaa ggaaggttag gatttataat ggtttgttta ttagcagctg
     6241 caatgccatt tatcgttatg acagggatgc atcatgcatt gactcctata ttcatatcag
     6301 catttgcaac tacaggacaa gaatcgctaa tattaatatc tcaagtttgt gctaatttag
     6361 cgcaaggtgg cgcgacatta gctgtagcat taagaagcaa agatagtaag atgaaacagt
     6421 tagcatcagc tgcatggata tcagctatca tgggtataac tgaaccagct ctttatggag
     6481 ttacattgaa acttaaaaaa cctgctatgt tagcatctat aggagcagga atcgcagggt
     6541 tatttgcagg aattactcat gtgactttat atgttccaca aaatagtata atggcagtac
     6601 ttggattatc aggggaaaag ggaatttcaa atatagtaaa tggacttata gttatattta
     6661 tagcattggt tgctccattt attatgacat atgtttttgg agttgaaaaa aatgttaagg
     6721 aaagtacaga taatgtagta gaggaagtat aatattaact atttacatta atagaatttt
     6781 tattattatt gactataact tgttttgtat ttatattgat gcaaagaaat aagttaatat
     6841 taatgataat aattattata actgacaaaa agcaacacaa aagattgatg aagtatatgg
     6901 aaataaaaag ttgacaaaat atgtctgatg ttttatcata ttaattggaa ttaggtagta
     6961 attgtttaag tacaagctaa gcttattcat taataatagc agtatcctag gaaggagtat
     7021 gcccattatt atggataagc tttttttatg cttaaaagga ggataatcat gataattaat
     7081 aagatattaa acaataatgt tgtaatcaca ttagatgata atgatgaaga agttatcgtt
     7141 atgggtaaag gaattggtta tcaaaaatca aaaggcaatc taatagataa aactaaagta
     7201 aataaagtat ttagaatatc aaataaagaa atatcaaaca aactacaaga attactcaat
     7261 aatattccta tagaacacat gaaattatca agtgaaatta tagaatacgc acaaataaag
     7321 ttaaacaaga agctaaatga aagtatttac atatccttaa gtgaccatac atattctgca
     7381 attcaaagaa tgaaagaagg aataaatgta aaaaatgcaa ttctttggga aattaaaaga
     7441 ttttataaag aagagtttga aattggaatg aaagcactag atattattga aaacaaaaca
     7501 ggtataaaat taccagaaga tgaggctgga tttatagcct ttcatatagt gaatgctcag
     7561 ttaagtgaag gtcatacttt ggcatctgat attacgaagt taatacaaga agtacttagc
     7621 atagttaggt atcattttag gatagaattt catgaagaat cagtgtttta ttatagattt
     7681 ataatgcatt taaagttttt tgcgcaaaga ttgcttttag atagtgcaca tgaaggtgag
     7741 acagacaaag aattactaag cattattaaa tctaaatata ataaagagtt tgaatgtgta
     7801 gttaaaatta aaaatttcat taaaaaacaa tataactaca tattaacaga tgatgagctt
     7861 atatatttaa caatacattt agctaaagta gttaaagact caaatatgta agtgaaataa
     7921 tttttcagta gtatattaag aatgattgaa aggagtgata ctaacaaaat tatacctatg
     7981 gttggatggt aacattgagt tggccaaacc ttcaaatttc aaaaaattaa tttaaacgaa
     8041 atatggagga gaaaaatggg aaaatacaaa caattagcac aagagatagt aaacaatgtt
     8101 ggtggaaaag aaaatattag tggattagta cactgtatta caagattacg ttttacttta
     8161 aaagatgaaa gtatagcaaa tgacaatgta ttaaaaaaca tggaaggtat tgttactgta
     8221 atgaaaagtg gaggacaata tcaagtagtt ataggaaatc atgttgaagc tgtctataaa
     8281 gatgtagttg aaataattgg tttagatgat tcaagtacaa gttcagaaac taaaaaatca
     8341 ggtaatatac tagataaagg tatagatata atatcaggaa tttttcagcc agtgttagga
     8401 ataatggcag cttgtggtat gctgaaagga tttaatgcat tatttgtagc aatgggatta
     8461 tatagtgatg tatctggtgg ttatatggta ataaatgcag caggagatgc attatttaca
     8521 ttccttcctt tgttcttagg atatacttca gctaagaagt ttggattaaa accaatgttg
     8581 ggattagcac taggagctgc agtatgttat cctgctatac aaggtagcag tatatcagca
     8641 ggagcagatg tgatgtacac tttatttaaa ggaacaatgt ttgcatcacc agtatatatt
     8701 gatttctttg gactaccaat tgtatcaatt gattatactg gaacagtcgt accaattata
     8761 tttatagtgt attttgcttc taggtgtgaa aaattattta ataagtttgt tccagattta
     8821 gttaagtttt tctttgtgcc aatgcttaca ttattaataa cagtgcctgt tgcacttata
     8881 gttataggtc ctatcgcaac atttggttct acacttattt cacaaattgt aatttcaata
     8941 agagatttta gtccactatt ggcaggtgct atagtagggt ttacttggca aattcttgtt
     9001 atttttggta tgcactgggg atttattcct gtatatataa acaatgttat gactttagga
     9061 tatgataatg ttatgatgcc attctttgct tgtacatttg caagttcggc agttgtgttg
     9121 gcaatatttt tcaagactaa agataaaaaa ctaaaagaga tgacaatacc aaactttata
     9181 tctggtatat ttggagttac agagccagca atttatggaa tattattgcc tttaaagaaa
     9241 ccatttataa taagttgtat tgctagtggg attggaggcg ctttctacgg attctttaat
     9301 cttagaaaat ttataactgg aggtatggga atatttgaac ttccagcaat gattgaacca
     9361 gatgggggaa tgggcaactt aatagtagct ctttctggta tagctatatc aatggtcgta
     9421 gcttttgtat taactatgat tttatataaa gatgagaaag tagaagagtc taaaaaagta
     9481 agaaataagg ggaaagaaga aataaaagaa gtcaagtcta ctaaattaga aagagaaata
     9541 gtagcaagtc ctataaaagg tgaagtatta aaattaagtg atatagagga tgcagcattt
     9601 gcatctggtg tactaggtca aggggtggca ataataccta gtgatggaaa agtagttgct
     9661 ccagtagatg gtgtagttac aaccttattt cctagtctac atgctatagg gattctttct
     9721 gatacaggtg ttgaagtgct tattcatatt ggattaaata ctattcaatt agaagggaga
     9781 ggttttgagg catgtataaa acaaggtgat agaattacaa aaggtcaaac tatattaaac
     9841 tttgatgttg atgcaataaa agagttgggg tatagtactg taactccaat tgttattaca
     9901 aattcatcac aatttttaga tgtagtagaa acagaaagta aaaatattga attagaagat
     9961 aatctaatta cagtcttatt ttagtaaaaa ttgaggcatt agcataagct agtgctttgg
    10021 ttataattta aggaggcttt gactatgagt tttaaggatg attttttatg gggaggagct
    10081 actgcagcca atcaatgtga aggtggatac aatgaagacg aaagaggatt ggcaaatgta
    10141 gatgtatgtc caacaggaaa agatagaaca gcagttatta cagggaaact aaaaatgttt
    10201 gattttgatg atgagcatta ttatccagct aaaactggta ttgatatgta tcataattat
    10261 aaagaagata taaagttatt tgctgaaatg ggatttaaag tatatagaat gagcattgca
    10321 tggagtcgaa tatttccaaa aggtgatgaa gaaacaccaa atgaaaaggg acttcaattt
    10381 tatgaaaata tatttaagga gtgtaaaaaa tataaaatag agcctttagt cactattaca
    10441 cactttgatt gtccaatgca tttagttaaa aagtatggtg catggcgtaa tcgaaaaatg
    10501 ataggtttct atgagaaatt atgcaatgtt atctttagaa gatatagagg attagtgaag
    10561 tactggctaa cctttaatga aataaatatg attttacatg ctccatttat gggtgctggt
    10621 atttgctttg aagaaggtga agatgttgaa aagataaaat atcaagctgc tcatcatgag
    10681 cttgtagcta gtgcaattgc aactaaaata gcacatgaag ttgattcaaa taacttagtt
    10741 ggatgtatgt ttgcagctgg aagtgtatat ccttatagtt gtaatcctaa tgatgtatgg
    10801 gaagctacaa agttagatag agaaaattac ttctttgtgg atgtacaatc taaaggtaaa
    10861 tatcctaact atgcgttaaa gtatatggag caaaaaggta ttacaccaga aatggaacct
    10921 ggtgatattg aattattgaa taaatataca gtagatttta tttcattttc atattacaat
    10981 agccgttgtg ttagaactga tgaaaatgca gatgatatgg ctgaaggaaa catatttaca
    11041 tctgctaaaa atccatatct ttcatacagt caatggggat ggccaattga cccattaggt
    11101 ttgagaatta cactaaatca tgtatatgat agatatgaaa agccactatt tattgtagaa
    11161 aatggattag gtgctaaaga tatagctgat gaaaatggtt atgtagaaga tgattacaga
    11221 attaattatc taagagaaca tataaaagct atgcatgatg cagtttctat agatggggtc
    11281 gatttattag gttatactac atgggcacct attgatttag taagtgctgg tacaggtgaa
    11341 atggaaaagc gttatggatt tatatatgta gatagagata atagtggtaa tggaagtcta
    11401 agaagaatga aaaagaaaag ttttgaatgg tataagaaag taattgcttc aaatggtgaa
    11461 gatttatatt aaacaagaaa tctatgttaa ataagaaatt tgtattaaat aagaagagta
    11521 taaaaattaa taataagtat aatttttaac ttttttaact atagaaaatc aatacagaat
    11581 tattttacat tgtatttatg caatagtatg attcacaaat aaaattttag aaaataaaat
    11641 tgttaagaag tatgggaagt aagtgcaatt tactttgtaa taaaagatga aaaaggcgag
    11701 cttaggtttt aagcttgtct tttttattat gtataaacta tgatacttta aaattctatg
    11761 aaaaatttat ttaatagaga aattaaaaat ttaatggtaa tcaaattctt taccaatgaa
    11821 tataaaaaat gtctacgtgt tataatagtt taaactaata aacgaaaggt agatgaatga
    11881 atatgaaata ccaacatata atagaagttg acaaagaact ttgcattgga tgtgggcttt
    11941 gtaaaaatga ttgtccagta aacaatataa ttatagaaaa taaaaagtca gtaattaaaa
    12001 aacaagattg tctaatgtgt ggacattgtg cagcaatatg tcccacaaaa gctattacct
    12061 tgacaggatt tgatgaacct cctatagaac ttacaaataa gccaaagcta gattcagatg
    12121 aattactaat ggcaattaaa tctagaagaa gtatacgtaa atttaaagac aaagaagttt
    12181 cttcagaaat tgtaaagcaa attattgagg caggtaggta tacccctagt gctaaaaatt
    12241 cacaagatgt atcatatatt gttttagaca ataaaaagtc aatttatgaa aacgaagcag
    12301 tgaaattttt tagaaaaata aaacctattg caaatatagc tattaaatat tcaaaagaag
    12361 ttgagattga cgataacttt ttctttaagc atgctcctat tgcaataatg ataattacaa
    12421 aagataagat tagtggttca cttgcagcat caaatatggc attaatggca gagtcttatg
    12481 gtctaggtgt tttatttagt ggatattttt cagatgtagc aaataattcg ccaaaattaa
    12541 aaaagctttt aggtctaaaa cgcagtaatc atgttcttac aacattagta attggatacc
    12601 cagatgttaa atatagacgt actgcacaga aagaagtagc aactgttaga tacttataag
    12661 agagggtgga ttttatggaa aaagaatgtc aacaaagaat gcaagaaata acatctaaat
    12721 ttaaagaatg tcgcaaagct atctctgcta tgggtgatga gactagacaa ttgattttaa
    12781 ttgctctctt agagagtgat tttaatggta tacgagtggg agaaatcaca aaaaaaacac
    12841 atctttctcg tcctgctgta tctcatcatc ttaagatatt aaaagaagca gagattgtta
    12901 atttgcgtag agaaggaacg aaaaattatt attatcttga ttcaaaaaaa tcacaatgga
    12961 agtcactaac agagctaata aatcttatat ttgttggaat acaacatatt agtgaaaatg
    13021 atataagaaa aggtgattaa attatacttg atttaataca aaaagattga tttaggaact
    13081 aaaaagttca ctttgagaca ctatcttttg aactataccc atcttaggag acagttaaaa
    13141 tatgtaaaac tgtataaaac aatgggtata gttcatttta taatggttta aatattaaga
    13201 atatattatt ctaaatagta tctttctctg ttttgagttt tatttttata ctcaatagct
    13261 tcagtggggc aaccacaaat acatgccata caatgagtac agctattttt ccaaactggc
    13321 tttttgtttt ttaaattaat attatttaaa ggacatagct ctacacattt tccacagcca
    13381 atgcatttat cagtataatg aaatcctttg gcactaataa ttgtcttata aaaaactgta
    13441 tttattatac cacttttaat tgtacctcct aagccactag gagtaatagt ttcaaattca
    13501 ttttcattgc gtatatcaga aataatccta tatatttgtt tatctgcttc tttaatcatt
    13561 tgttttgatg tttctttgtc tgttgtagaa aataaagcta tataattctc tggcatttta
    13621 atttctgcca taccttttaa ttgccattcc ttgtatttac atagtttttg aatatagttt
    13681 atggattttg ctgtatctcc cccacaagtc attacaaagt aaactctgtt gtctcctaga
    13741 aattttgttt cttttataaa atctgtaacc actattggca atctccatgc ataagtagga
    13801 catacgaata tgaatggttt acctgctgat attaattcat ctgttttttc ctcttttata
    13861 agttgattta gtgatatcag ttcatcattt aattcttgtg caattttacg agccacatat
    13921 cggctattac cagtaccgct aaaatataaa atcataataa tatctcccta actatttttt
    13981 attttaactc ttaatttttt atttattata gtactttatt gaaatattct attcaaatgt
    14041 atgtaaaatt tagtagttaa gaaaattata acacattagt aatttttata ttcattggta
    14101 aagaatttga ttaccattaa atttagtaac aaaatatcaa ctggattttt atataagaag
    14161 agatagtaat tttcatgtga aagattgtaa atataaagat gattttgaag ggcaataaga
    14221 agataaaatt gatagcttaa taaaaaatat ttaactttta tattaactgt tttaaatatt
    14281 ataattatct atcttgaata atcaaccata gaagactgta tacaaagaca aagttgtgta
    14341 cagtcttttt tataaattta atgtctataa ttaaatacac tattaaaatc aataagttat
    14401 tatttcaaag tagtattatc caaattttta tgatggttaa tacaatttgt tagccatttt
    14461 atagcacctt gttccctgat aatagctcca tcaagaacta tataatcacc aaaatcatta
    14521 ctatcaagag gaggaacttc actataacgc tccatttgac tgtacaagaa cgtaattctt
    14581 tcttggtgtt gaagcaattg atgctctagt aaaataagcc tagtatctat atctaaatta
    14641 ttagaaaaat acattctaag acgaaaaata tccttagggg ttctttccat tggctcatcc
    14701 ttttcaagcc atttcaagaa ctcatcttta cctttttctg tgattgtgta taactttttc
    14761 tctaatacat caccactaat tttaatatca tatacaataa gcttttcatt taccaatttt
    14821 tttagttcag gatatatttg actatgcttt gcattccaaa actcagctaa ttgaaaattg
    14881 aactccttta cgatatcata accagtcata ggatttctat ttaataatcc taaaattgca
    14941 tactttaaag ttcgcataaa tacacctctt tctttaatta catacttatt ataacacgat
    15001 atatttagtt tgaattaatt aatatagcta tgttttaata agaactttta aattatgagg
    15061 aacaaaaaat aaataaaact atatataaaa atgaaa
//
LOCUS       DAEPOG010000097        15085 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEPOG010000097 DAEPOG010000000
VERSION     DAEPOG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.097"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(59..949)
                     /gene="metA"
                     /locus_tag="KR432_002365"
     CDS             complement(59..949)
                     /gene="metA"
                     /locus_tag="KR432_002365"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH0640895.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(979..2244)
                     /locus_tag="KR432_002366"
     CDS             complement(979..2244)
                     /locus_tag="KR432_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBH0640896.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(2354..2456)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2510..5167)
                     /locus_tag="KR432_002367"
     CDS             complement(2510..5167)
                     /locus_tag="KR432_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0640897.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(5295..6038)
                     /gene="yaaA"
                     /locus_tag="KR432_002368"
     CDS             complement(5295..6038)
                     /gene="yaaA"
                     /locus_tag="KR432_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH0640898.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(6048..6512)
                     /gene="bcp"
                     /locus_tag="KR432_002369"
     CDS             complement(6048..6512)
                     /gene="bcp"
                     /locus_tag="KR432_002369"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH0640899.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(6864..8561)
                     /gene="ade"
                     /locus_tag="KR432_002370"
     CDS             complement(6864..8561)
                     /gene="ade"
                     /locus_tag="KR432_002370"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH0640900.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(8867..10021)
                     /locus_tag="KR432_002371"
     CDS             complement(8867..10021)
                     /locus_tag="KR432_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBH0640901.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(10266..11105)
                     /locus_tag="KR432_002372"
     CDS             complement(10266..11105)
                     /locus_tag="KR432_002372"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH0640902.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(11118..11714)
                     /locus_tag="KR432_002373"
     CDS             complement(11118..11714)
                     /locus_tag="KR432_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0640903.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(11765..12415)
                     /locus_tag="KR432_002374"
     CDS             complement(11765..12415)
                     /locus_tag="KR432_002374"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH0640904.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(12461..13714)
                     /locus_tag="KR432_002375"
     CDS             complement(12461..13714)
                     /locus_tag="KR432_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0640905.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(13738..14379)
                     /locus_tag="KR432_002376"
     CDS             complement(13738..14379)
                     /locus_tag="KR432_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0640906.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(14581..14994)
                     /locus_tag="KR432_002377"
     CDS             complement(14581..14994)
                     /locus_tag="KR432_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBH0640907.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
BASE COUNT         4697 a         2721 c         1620 g         6047 t
ORIGIN      
        1 aactttatac ctataaattt ctatattttt ttagttaaca tttatatact cttaaaattt
       61 attgtatcaa atagtttttt atccaatttc taaacaacaa ttctgaatgt tttttccatt
      121 ttacagttgg ctcttctgaa gggtcatcat taatatagta attttgaggt atagatattt
      181 tatctccttt atttatatcc ctcttatatt ctttatcaag agtgtatttg tcatactcgc
      241 tatgacccat tacgaacaca tctcttgaat ttgtaattat atagggacca gcttctttag
      301 aatgactcac aatgctcaac tctttattct ctttcaaagc ttcaatattt acagtagtat
      361 gtcttgaatg tggagcaaaa aattcatttt caaatccatc tactatttta ctatctttat
      421 ctaccttatg cttaaatact ccaaaacatt tttgacttag tggaagtttt tttacattgt
      481 agtatttgta aagacttgct tgagctgccc aacatatata taatgtagat ttagtttttc
      541 tcttagaata atccataatt tcttctagtt cattccaata atcaacttct tcaaactcca
      601 tttgttcaac tggagctcct gttattataa agccatcaaa atactctcct ttggcttcat
      661 caaatgtttt ataaaattct ttaacatatt cattacaact tcttttactt tctcttgttg
      721 aaattttaat aaattcaaca gacacgttga atcctgtttt atctatcctt cttagtaaat
      781 ctaattctgt atctatcttt ataggcatta gatttagtat agctatttta gtgtctatgt
      841 tttcttcata ctttaattcc tttttaaaat cttctttata aattacatct attccttcat
      901 ctaataattt attaataaca ggtaatccct ttggtagtat taaagccata tcttttcact
      961 tctctttctc ttagaatatt agaaatttaa agcttgattt aaatcagcta ttaaatcctc
     1021 tacattttct attccaacag ataatcttat tagtgaaggc aatactccag atgcaattaa
     1081 ttgttcttct gttaattgtc tatgagtagt tgaagctgga tgtataacac aagttcttac
     1141 atctgaaaca tgtgtaacca aagatgctat ctgtaatttt tccacaaatt ttttggcatt
     1201 ttctaaccca cctcttactc caaatgataa aacaccacta catcctcttg ataaatactt
     1261 tttggcattc tcataatact tgttatcttc aagtcctggg taattaatcc aatctacatt
     1321 ttcatgtttt tctaaaaatc tagctatttt aagtgcattt tcactatgtc tctccattct
     1381 aagatgtagt gtttcaacat ttaaattagt aagatacgca ttgaatgggc ttaaacaatt
     1441 tccatagtct ctaagcaact gaactcttgc tttagttgca tatgcggcat ttccaaattt
     1501 ttgagtatag cttattccat gatatgttgg gtctggctca actaattctg gaaattttcc
     1561 attatcccag ttaaagtttc caccatccac tataattcct ccaacacttg aagcatgccc
     1621 atctaaatat tttgtggtag aatgagtaac tatatttgct ccatacttta aagggttaca
     1681 aagcactgga gatgctaatg aattatcaac aataaatgga acattaattc tcttagctac
     1741 atttgctatt ttttcaaaat ctatgacttc aagagtcgga tttgcaagtg tttctgcaaa
     1801 tacaacctta gtattttcct ttgcaagttc tactatctca tcttcacttg aatctaagtc
     1861 aaacgatatt aaatctatac caaatttttt aagactaggt cctagtaaat tgaatgttcc
     1921 tccatatact tttgaagaac aaagtatact atctcctgat ttacatatat ttaaaactgc
     1981 caacatattt gcagattgcc ctgatgatac agctacagaa gatactccac cctctagtaa
     2041 acttattttt tcttcaaaag cttgaatagt aggattgctt attcttgaat atatatgtcc
     2101 atcaactttt aaatcaaata attcagcaag ttggtctata ctgctatatt taaaagttgt
     2161 actttgatat aaaggtaata ctcttggttc tccattacct ggtttataat ttccttgcac
     2221 acatattgtt tctttattat acattcttgt atccccctta gattttagta aaaaaaatta
     2281 aactatttat cagatgagag ttaaaataaa taaagcctga gtatataact caggctctgt
     2341 ataagcttaa ttatactcat ctctcagctt tcgccgcagg atttagcacc aatgtatata
     2401 aatatactgg ttgccgggtt tcatagggcc agtccctcca cctctctgga taagatttat
     2461 ttaatttttc tatgttttat atcttatata tttttcctaa ttttgtcaac tatttttttc
     2521 ttttttttct tttaataatt ttcccaacct ctacttgaat tacagacatt atggctaaaa
     2581 caatcgttat aagccactct aagttattta aaactgttaa gttaaaagca tttctaaaaa
     2641 caggtacaaa cagtattact gctattaaaa atgcagaagt taaaaatgag attaaaagaa
     2701 atggattttt accattattt ctattaaaca ttgaatctgt atttgaacgc tgactaaatg
     2761 ctcgtaacat ctgtgaaaat gctaatacac aaaatgccat tgtttgacct acagaatgat
     2821 tttctatatt aagaccaatt ttatatgcta aaatagtcat tattgccaca aatatacctt
     2881 gggtaacaac tctttgtata agattttttt caaacaaagt ggttgatttc actggctggt
     2941 gcttcattat atttctgctt ggtgggtcaa ctccaagagc aagggctgga agcgtagcag
     3001 ttgcaaggtt tacccataga atatgaacag caagaagcgg agtatcccaa tttaataata
     3061 ttgctacaaa caacgtaagt atctctgcta tattaccaac aagcaaaaat tgtataactt
     3121 tttgtatgtt tctatataca cgtctacctt cttttattgc ataagcaatt gttgtaaaac
     3181 tatcgtccat taaaatcatg tcagaagaat cttttgcaac atctgtacca cttataccca
     3241 tggctatacc aatatcagca gactttaagg caggagagtc atttacacca tccccagtca
     3301 ttgctggtac ttctccaatt ctttttagag attgtataat tctaagttta tcaaacgggg
     3361 acacacgagc aaatactgta gtatttttaa cagcttcatc tagttcatca tcactaaaat
     3421 tatcaagttc atcaccagat atcacagtat tttcttcact ccatattcct aactcatgag
     3481 cgattgttgt ggcagtaact ttatggtcac ctgttatcat tatggttctt atacctgctt
     3541 ctttacaagt acttactgca tcaattacct cagttcttgg tgggtcaatc ataccaacca
     3601 tacctataaa tgtcaaatca tattctatat tttcactatc ttcttttggt atagatgaaa
     3661 tctcacgctt agcaaaacca agtacacgca atgcatcttt agacatgtta agacataatt
     3721 cgtgtatatt ctttttatcc acttcggtaa tgtttctttc accttttgaa gttaatattt
     3781 tagtacatag gtcgagaatt tcgtctactg ctcctttagt ataagcaata atcttttcat
     3841 ctatattatg aacagttgtc atacattttc tatctgaatc aaaaggctgt tcaaatacac
     3901 gtggatattc ttcttcaaga gcttcttgat ctattccaaa ttttttggcc ataaaaatta
     3961 atgcgccctc tgttgggtca cctaatattt catctgtttt atctggatta atacatgcat
     4021 cattacaaag tgcaccagca taaacaatct ctttatacac taatggatgt ttttcagatg
     4081 cagtatctat tgatgtcact tttccagatt caaaatctcc attgacagca atatgtgtaa
     4141 cagtcatttt atttaaggtt aatgtaccag ttttatcaca acatattact gtagctcctc
     4201 ctaatgtttc aacagcaggt aattttctaa ttaaagcatt tcttttagcc atacgctgta
     4261 cgcctaaagc cattacaata gtagctgttg caggtaatcc ctctggaata attgatatgg
     4321 caagggaaat tgcaatcata aacattggca agagaggtct ctgataaaat gcacctattg
     4381 caaatataac aacgcataca ataataccta tgacacttaa tgtctttcca acagtattaa
     4441 gtttacgttt tataggagta tcaaaatcat cttgtgaatc taaaagatgt gctatgttac
     4501 ccacttctgt atccatacca gttgctacta caacacctac acctctacca tatgtgacta
     4561 ttgaagatgt atacgccata ttgctacggt ctcctaatac acattcttta ggtaagattg
     4621 tatctgcatc tttttctgat ggaacagatt ctcctgttaa agaagcctct tgaattttta
     4681 aacttgatgt ttctattaaa cgaaggtctg ctggaaccat agctccatct tctaaaaaaa
     4741 caatatctcc tactacaaga ttacttgctg gaataacatt ttcttcacca tcacgcataa
     4801 cacgagctgt aggtgagttc atatttttta atgcttctaa tgatgattct gcttttttct
     4861 cttgaacaat tccaataact gcatttactg ttacaattat tagtattaca aaagcttctg
     4921 taaattcgcc aaatatcaaa gataatattg ctgctcctat tagtatcata accatagcat
     4981 ctgtaatttg actccaaagc atttgtaaag ttgttctagg tggttttttt ctaagttcat
     5041 ttaagccatt ctctttaagc cgtttttccg cctcggtatc ggtgagcccc tcctctgatg
     5101 aatctagact ttctaaaatt tcatgcgttg ctcttgaatg ccaaggcatc tgtttgtgat
     5161 tttccatttt ggaagaccac tcctttcttt ttttaattta agaataatta tattttaaaa
     5221 tcaagttcaa ttcaatacac actaagtata ttttacctaa atttaacaat gccatttaat
     5281 atttaacaaa acttctactc ttttacaaat atgtattcac tattgttaga taagttttct
     5341 ttatacttat aaccttctaa attaaacttt ttcagttctt caatatctct tatcctattt
     5401 tttacaatgt attttgtcat catacctcta gctttctttg cttgtgtaga ttccactttc
     5461 aatatgccat ttttatttac tttaaaagta catgtaatat atgtatcact atctttaata
     5521 aatttttcta tggacttgct gtattcattt gatgaaagat taatgatagc tgttttttct
     5581 ttgattagtt ctttatatat acgatttccc caatattcgt ataaattctt aaactcacct
     5641 actcgtaatt ttgtctgcat ttctaatcta tattcatata tactatcata tggttttaca
     5701 acaccataca atccacttat gattcgaata cagctatttg caaactcttc atctttcctt
     5761 gtaaaatttt ctgcttctat atttttatat tgtattccat cataagttaa gattgcaggg
     5821 tttcctaatc tatcaaattt aatgctttca aatctatttt tatttaatac agctaatttt
     5881 tcattaattt tcattttatt tttaaaatct tctataccaa atgtctttat attttccact
     5941 atttcttttg tgttatctat aaaacaaggc aaactcaaat ctctatcaaa tacttctata
     6001 ttcttcatat ttttagctgg tgataacatt ataatcataa attcttatta ctcctgtttt
     6061 tccaaatatt caagaatctc ttgtgcattt gtgtctggtt ttgctttttc aaagactttt
     6121 tcaataattc cattttcatc tataatatat gtcgcacgta caactcccaa tgcagttttt
     6181 ccatacattt ttttctcttt ccatacatca aaagctttta tagcaactaa gtcagggtca
     6241 gaaagtaaaa taaaaggaag ttcatgcttt tctgcaaatt tttgatgtga ttttatacta
     6301 tctttactta tcccaataac ttgtatatct tcctttttaa atccatcata tgcatttcta
     6361 aaagcacaag cttgacgtgt acatcctggt gtattatctt taggataaaa atatactact
     6421 acttttttgc ctttaaaatc tgacatactt accttatttc catctttatc ttctagtgta
     6481 aattctggtg cttttgttcc tatactcaac ataaatatct ctccttttgt ttttattaat
     6541 aattttatac tatgcttata ttattaccct ataaatctat atataaataa aatattttat
     6601 tttttatgta taaagtttca taaatatata attttcatgt atttatattc taaaatagac
     6661 tgaaaatagt tttattaaga tatctatgct taaaacaagg ttaaatgata gattaattaa
     6721 aataaggata tctaaaaata aaatttttag ttcattagat gctctttttt gtatgaaatc
     6781 aaatatattt tcatcatttc aacaataaaa agagacttat ccctattttg agaaagcctc
     6841 atttattatt acttttatat tatctatata aatttaaatt cctcaacatc aaatagacct
     6901 aaatctgtaa gtcttatttg tggaacaact ggaagtgcca taaaagaaag tgtaataaaa
     6961 gggtctacat actctggaac tcccattttt cttgctatct ccaacatatt atcagtaatt
     7021 tcttgtactt cttcagctgt aaaagtacta attaatccag ctacttgaag acttaggtgt
     7081 gcaagaactt ttccatttga tacaattaca taaccacctt gtatttcctt taagtaattt
     7141 atagctacta gaatatcatc atcattatcc cctgcaacta taatattatg agaatcatgg
     7201 gccacagtag tagcaattgc tccatttttt atgccaaatc cttttagtga agcaattgct
     7261 acatttccag tcattctatg tctctcaaca acacaaagtt ttgaatactc tttatttgga
     7321 acaaaatatc catctgcaca tggtaaagat tcatatacct tatttgtcaa aatttgataa
     7381 ggtacaatct ccattacata atttttttca cttactttta actgtatttt ttctttatta
     7441 atatacttaa acttgactgt attaagcaat tctttatctt taatctcata attgtaatta
     7501 gaaaacatct cttcgtttac caaattacca tctttatata cactatccac ttccattttg
     7561 tctaaatcat tcaatacaac aatatctgcc ttataacccg cacctattgc tccaattttc
     7621 cttaacccat atgcttttgc agaattataa gttgcaactt ttatagccct aacaggttcc
     7681 atacctaaat caatcgcaca ttttatattc catcttatgt gaccttgttt ttctatatca
     7741 tctaagtgct tatcatctgt gcaaaacata aattcttcaa ttggcaagtt atgctttagc
     7801 ataccactta ctatagactt taaatttttt gctgcgcttc cttctcttat taaaattttt
     7861 aatcctgctc tcaatttttc atatacttca tcaaaagtta tacattcatg gtcattttct
     7921 attcctgcac atacatatgt ctgaagtgat ttaccattta tattaggtgc atgtccatct
     7981 acaactttat ttttaaaaag ctctagttta tccaagattt cattttcaga atttataaca
     8041 tcattaaaac acattacctc tcctaaacct aaaattctag gattatttac ataactctcc
     8101 atgtcttttg cactaaattt accaactcca ttagtttcaa aacttgttgc aggtacggat
     8161 gaaggcacca taatatatac attaagtggt atatcttttg tcgattctaa taaaaaatct
     8221 attgcactag ctccttttac attgaccaac tcatgtgggt cggctatgat agttgtcgta
     8281 cctgatttta ataatttttt cgataattca attggcatta ccattgtaga ttcaatatgc
     8341 atatgtgcat caataaagcc aggtgatata tatttattgc tacaatcaat ttctacattg
     8401 cccttgtaat cgccaatgcc aacgatagtg tctcctacta ttgctacatc tcctgttatt
     8461 aactcttctg taaatacatt tatgattgtc ccatttttta gtacaatgtc agctttttct
     8521 cttccacttg aaacatcaca ctgtcttttt aaattaccca tttttacaac tcctcctaag
     8581 ttttttatat caatatactg gctgtattac gtattatagt ttaatttatt aatatatatt
     8641 atatatgagt tttgatagaa agtatcactt gaaatttgtt ccataattcc tttaattaaa
     8701 tagagagaac atgaggaata atattatctc ccatgttctc attaatattt aagcaataat
     8761 ttaattcata ttgaattttt tatttttata tttaatcttc actattctta taaataaatt
     8821 tatgtgcttt attatttttt taatacttct ttattcgtaa ttatatttaa cctaaaaatg
     8881 cataaactat catacctaca agtattgctg gtatagtagt aataatcata gcccataaag
     8941 aagatttttt atccattgct aaaagtggga aaagtgcatc tccatcttga gaaatagagt
     9001 tggctatcac tgcaccaagt ggtatagttc ccttcaaata aaatgacata agcacaattt
     9061 gtatcccaca acctggaata agtccaagac ctgcacctat aaatacactt attacacctg
     9121 ttgataatac tagtttaact aagtattcat ctccacctaa aattaatatt atcaaatcgt
     9181 aaataagata tgcaataaaa atccatgtaa ttacaaatga tatttctcca acagaatgga
     9241 taagcatttc tcgcaaagaa atctttttat tttcttcctc tttttgatta gaatttttaa
     9301 ataccttttt aaaacaccac atataaatta ttgaaaataa tattccaatc actgctatta
     9361 cttcttccaa tgaatgtaac ttctcaatta taggaagatt aagtccagag tgagcaagtg
     9421 acataaatat aaaaccgatt actataattc ctatataaaa tttatatccc tttccatgtg
     9481 ttattataaa tgcaagtcta tttgtatttc catgagattt agctagagtg tctaaaatta
     9541 tttcatcatg attatgttgt tctttatttt ctttagggtt atttttactt atatcatatt
     9601 tatctgattt tctacggttt tttaacccta atttttcttc tagtttaaag taatccacta
     9661 attgtcccgt tattattcct gtaattgtac taactatagt gacaaaaaaa tacattttaa
     9721 tatttgatga aataagtaca aatgctgcat ctcccatact agctattaaa cttgcaatta
     9781 ttgctccaaa gcttaatttt ccatttatat acaatggcat tattgccaga cttccgccac
     9841 aacctggtat agctccaatt aaagcaccta ttaagggttg aaattttctg ttttttgata
     9901 tgatattagt aaaatttcca ctagttttat aattaatata tccaaataac aaaataaaaa
     9961 atccaatcat agaacctaca tgtaaaaacg cctcttcaga agcggatatc aacatttcca
    10021 ttaatttacc tcctagacta attttgcaac aattatatta tattctattt tctattgata
    10081 atcaatatca atatttcttt tgaattttta atattaaaaa gtgcgcatct tatactctta
    10141 gtttttcatc ttaagataca cactttttga tattaggtta tctatttacc acatgtaata
    10201 tgaaatcaaa ggtggatatt atgataaatt ataacaatgt ccacctattt ttattaccta
    10261 tcaaattata aattttcaag gaatgttaca acttcttcta ttatccaatt aggtgtagat
    10321 gcccctgctg taatacccaa atttttatat tttttaagcg tatttacatc caattcatct
    10381 ttagtctcta ttgcaaaagt tggtactaag tcttcacaaa cctttactaa cttttgagta
    10441 tttgaactgt gtttacctcc tataactatc atacaatcaa cttctttagc taactcttta
    10501 gctgattctt gtcttgtttt tgtagctgaa caaattgtat cattaaatac tatttcttct
    10561 aatttatctg atagtttatt aactatactt atatacaact ctggatttat tgtagtctgt
    10621 gcaacaagac aatatttttt ggagttatta aattccatat tttccatttg ttctaaggtc
    10681 tttattatta tagctgaatt ttcacaccat ccatttatac ctataacttc tggatgcttc
    10741 atatctccaa taactattat ttcatagcct ttattatgat attcacttac tactgtatgt
    10801 attttcttta caaatggaca tgtagtatcc acaattttta attttttatc tttggcttcc
    10861 ttatatatat tttcaggaac tccatgagac cttattatca tattttcatg atttggtatt
    10921 gtatcaattt cattcacagt cttaagacct ttttcttcat attttgctac tgcttgcttg
    10981 ttatgaataa gaggacctaa agcatatatg ccagaatcat ttttttcaac ttcatcccat
    11041 gccattttca ttgctctttt tactccaaaa caaaagccag cattttttgc tatttttatg
    11101 ttcataactt taccccctta ttttgaatat ttaaagtaat tttgttttat cacttctaaa
    11161 atctcttgtg atatcctttc atggtcatca ttactcaatt tttccttaaa atgttcttca
    11221 aatgatattg gttctccaat atatacaatt actttactaa aaagtttata tttagatatt
    11281 attgatactg gaacaacttt agatttactt tttattgtaa atacagaaag acctgcctta
    11341 gccttaccaa aagaatctcc ttttattcta gtaccctctg gaaatattcc taatacataa
    11401 ccatctctaa ctgaccttaa aatagttttt attgtagata catctggttt ttctctattt
    11461 ataggcataa catgcaattt ttttaatata aatcccaatg gctttacttt aaacaattct
    11521 ttctttgcaa ttgcagctat ttccctattt tctatggcag cagccaagaa tatagggtca
    11581 agattcgatt tatgatttga tgctattact atatttcctc tatctggaat attctctgca
    11641 cctataactt catatttaaa aaatatttta gaaaaaccct taaaaatatt tattacaaat
    11701 ctataaaaat tcacgtttat ctcccttcaa cactacattt attttcatta tgatgcataa
    11761 aaaactaaag atttatttta gatacaactt cttctacaac ttcttctata gtttttcctg
    11821 tagtatctat ttctagtgca tcattagctt ttaccaatgg tgcaaattct ctatttgaat
    11881 caatttcatc tcgtcttatt atatcctcaa ttacttcttc taaagtagta ttatatcctt
    11941 tattacaaag ttctttatag cgtctatttc ctctttcttc tggagtagct actaaaaaga
    12001 atttgtaatc tgcatctggg aaaacatatg aacctatatc tctaccatct agtattactg
    12061 aacttttcat tcctattttt cgttgaactt caaccatcaa ctgtcttact tcttttattt
    12121 ttgctacatt agaaacatta ttgcttactt ctattgttct tatttcttca tttataactt
    12181 ctttgtccaa atatatattg ttatctttaa aatctatatc cgaattttca gctatttgaa
    12241 taacttcttt ttcattattg acatcaacac cactttttaa acacttataa gtaactgctc
    12301 tgtacatagc acctgtatct atataattta tatttagctt ttttgctact atcttggcta
    12361 ttgtactttt tccagctcct gctggaccat ctacagctat aactaaatta cccatatata
    12421 cacctctatt tattcatatt taattattaa aaaattatat ttatacactt atacccgcaa
    12481 gatatccagt tgaaaatgct atttgtaaat tatatccacc agtttctgca tctatatcaa
    12541 ttacttcacc tgtaaaaaat aagttcttaa ctaatttaga ttccatagta gaagaattaa
    12601 tttctttaac tgaaatacca ccagaagtaa ccataccagt gtttatacct gtagtcccat
    12661 tacaagttag cttcatattt tttatatact caataattct aatttcatct gccttagaca
    12721 aatcacttgc ctttttatca actaaagata atagagaaaa aatctctctt aagaaatttt
    12781 gaggtagtat ttgcttaaga ttatttagaa catttttatt tggaaatgct ctgattatac
    12841 tagagatttc atcagtactt aaatttggta agaaatctat atttaattct accttttcct
    12901 tttcaataat cttgttaata taagaagaca ttataagaac acaaggtcct gttatcccaa
    12961 aatgggcaaa tagcatgtct ccagattttg atattttcct tttcttaatc ttacaagata
    13021 tttccacatt cttcatggat attccttgta aatctttaat ccatttttcc tctattgtaa
    13081 gtggaactaa agcaggatat agtttgttta atgtatgtcc atgcttttta agaatcttat
    13141 acattgaacc atctgaaccc gtctttgaat aactcactcc tccagttgat ataataacct
    13201 tgtctcctag tatcttatct ccattatcta aaataatccc ttttattaga tactttgact
    13261 tgtccttatt agaatcatct tttagagcaa tttcttcagt tataatatct ataactttct
    13321 tattatacat tatctttaca ttgtgattga gtaaatcttt atttaatact tctattactt
    13381 ctatagattt atcattttta gtgtatactt tgtggtcatt ttctttttca atcttatatt
    13441 ccaaatttct actttcaaaa tacgaaataa ggtctttatt tgtaaatgta tagaaactac
    13501 tatagagaaa ttttttatta ctgacaactt tatcaaaaaa atcttctatc tctctattat
    13561 tggtaaaatt gcatctaccg ccaccagttg ccctaagttt tcttcctaat tctccatttc
    13621 tctcaactaa tattacttca ttattgtttt ttgaagctga caaagctgcc atcattccag
    13681 aagctcctcc accaactact ataactttac tcataaattt ctccttataa ttgattccta
    13741 ctccagtgca gaattattta tctttacatg attttttata tgacttgtat cattcatttt
    13801 tactataaca ggtccataat ctttaaattc tactatattt aaagcagtat tatcttgctt
    13861 aattctatat atattttcca tacctgattc taaaaatgac aacaacataa ccctcaatgt
    13921 aattgaatgt gtaacaagaa ttatattttt attatcatat ttttcattta gttcctttat
    13981 aaaagcatct actctttcct ttataatctt aagagtttcg ccttctggta tatttactaa
    14041 gtgtggttca tttctccatg ttgcatatat atctgaataa tctttttgaa tttcttttat
    14101 taaaaggcct tcccaaactc cgaatcccat ttctctaagt gcctcagttt tttgaacttc
    14161 aatattgagc ttatctccta aaatttgagc tgtttgcatt gctctcccaa ggtcgctact
    14221 aaaaatataa tcaattgaat acttgccaat atcttcagaa agttcttttg cctgttctat
    14281 accttgaggc gtaaggtcag aattaccatg accttgtgtt tttcccaata tattccaatc
    14341 cgtttgtcca tgtcttacta tataaaaagt atttcccata tgtcctccta atatatgcct
    14401 attttttatt aaatataaat tatcacttct caaaacttaa ttatatcata taatacttta
    14461 ctttgtttct attttataat ctaatctaac atattattta caataaatct catttgtatt
    14521 tctgcaaatt attgtactgt taaagaattt ttctaaattt ataaacatat ttctcttata
    14581 ttatttatat acttcttcaa atctgtattc ttgaattgaa ttgacatact tatctgtatc
    14641 tactttttct aaaaacatat ccaaaggtct tgcaaatatt cctgtatcat catataaggt
    14701 cttgtataaa actaaatcat tagtatcttc ttctttttta tgataaaggt ttccatctaa
    14761 atccctataa acatagatat cttcttctct ttcagtatgt ctaataacta atttatattt
    14821 atttctgttt tggattaaat tttctttacc atatatttca catatgtttt ctatatcatc
    14881 aatagccttg ctaagaccca ttgtagcata atatttccct ttaaaatgtt tatagataca
    14941 aggatatttt aactttcttt cactgatacc taagttattc tttaattcaa tcattttatt
    15001 ataatctccc atatctcgtc cctttctttt aaaaagtatt ttattctgaa ataagttact
    15061 tgttttatat tttatcataa tttaa
//
LOCUS       DAEPOG010000098        15015 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEPOG010000098 DAEPOG010000000
VERSION     DAEPOG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.098"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(270..413)
                     /gene="scfA"
                     /locus_tag="KR432_002378"
     CDS             complement(270..413)
                     /gene="scfA"
                     /locus_tag="KR432_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBH0640908.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(479..841)
                     /locus_tag="KR432_002379"
     CDS             complement(479..841)
                     /locus_tag="KR432_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBH0640909.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(899..1192)
                     /gene="yajC"
                     /locus_tag="KR432_002380"
     CDS             complement(899..1192)
                     /gene="yajC"
                     /locus_tag="KR432_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH0640910.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1326..2447)
                     /gene="tgt"
                     /locus_tag="KR432_002381"
     CDS             complement(1326..2447)
                     /gene="tgt"
                     /locus_tag="KR432_002381"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH0640911.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2526..3011)
                     /locus_tag="KR432_002382"
     CDS             complement(2526..3011)
                     /locus_tag="KR432_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640912.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(3023..4048)
                     /gene="queA"
                     /locus_tag="KR432_002383"
     CDS             complement(3023..4048)
                     /gene="queA"
                     /locus_tag="KR432_002383"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH0640913.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4366..5385)
                     /gene="ruvB"
                     /locus_tag="KR432_002384"
     CDS             complement(4366..5385)
                     /gene="ruvB"
                     /locus_tag="KR432_002384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH0640914.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5492..6094)
                     /gene="ruvA"
                     /locus_tag="KR432_002385"
     CDS             complement(5492..6094)
                     /gene="ruvA"
                     /locus_tag="KR432_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH0640915.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6087..6596)
                     /gene="ruvC"
                     /locus_tag="KR432_002386"
     CDS             complement(6087..6596)
                     /gene="ruvC"
                     /locus_tag="KR432_002386"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH0640916.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6908..7537)
                     /locus_tag="KR432_002387"
     CDS             complement(6908..7537)
                     /locus_tag="KR432_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640917.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7740..8063
                     /locus_tag="KR432_002388"
     CDS             7740..8063
                     /locus_tag="KR432_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0640918.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8331..9185
                     /locus_tag="KR432_002389"
     CDS             8331..9185
                     /locus_tag="KR432_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640919.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9371..10807)
                     /locus_tag="KR432_002390"
     CDS             complement(9371..10807)
                     /locus_tag="KR432_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBH0640920.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10878..12260)
                     /locus_tag="KR432_002391"
     CDS             complement(10878..12260)
                     /locus_tag="KR432_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0640921.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12396..13301)
                     /gene="garR"
                     /locus_tag="KR432_002392"
     CDS             complement(12396..13301)
                     /gene="garR"
                     /locus_tag="KR432_002392"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH0640922.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13697..14362
                     /locus_tag="KR432_002393"
     CDS             13697..14362
                     /locus_tag="KR432_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0640923.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            14470..>15015
                     /locus_tag="KR432_002394"
     CDS             14470..>15015
                     /locus_tag="KR432_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640924.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4952 a         2632 c         1735 g         5696 t
ORIGIN      
        1 taaaatcatc ataaattaga gtttaaaatt aaaaattatt ctaatacatg taattgataa
       61 aaaataaaat ataagagtag ctactttaaa ataaatgctc ttatatgatt agatattttt
      121 gcataattaa acgataagaa atcaaattta attttacata agtagaatat atatttttat
      181 ataataattg aaaataaaag aggatacctc ataatgataa aagaatcact ttgagatacc
      241 ctcttacaaa caactaaatc aataattttc tatctttcac actcttgatt tgcaactgta
      301 caagaagttt tacaagctga ttgacaagaa gtttgacact ctccacatcc accttttgca
      361 gcactttgct ttaaagtagc ttgagataaa gtttttacat gtttattttc cattttcaac
      421 gcctccattt ctaattgttt cctattgatt atatcataaa actactttaa tataaactct
      481 atttaaagtt gacacccaat acgcctccaa tgcctcctgc tgctgatatt agcaataact
      541 tataaatcat ccctacttca aatacaaact tttcttgtgc taaaaacaca gtcaaaaaaa
      601 tacatactat gtacaaaagt cctactaaaa gtccatacat aagtcctttt tctttcatgt
      661 gttttgaagt gtaaaaccct cctatagctg cagaaatagt agttataaat gatgagacca
      721 tagttatatt atctccacca atatctgtaa atgtcaaaaa taagttatac actaataaaa
      781 cagccaaagt tattatatat gcatatccta gacctttaaa aatataatta gattttccca
      841 taaaaaaacc tcctctcaaa ttaatatata ttcgagaaaa ggcttttaaa ttactaattt
      901 agtcttcttc tatttcttct tttttagatt taacagttcc tatagcccat ttttcaaatg
      961 gaactttagt tctatttggt cctaattcta atattacaac atcatcttca actttagcta
     1021 cattagctac tattccacct attgtaatga ccttgtctcc tacacttagg ctacttctca
     1081 tttcttttaa ttgtttgtct tttttctttt gaggtctaat cattaggaaa taaaagattg
     1141 cgattactac tacccataga cctatactca taattatttg ttgctgtggc attaattttt
     1201 cactcctttg taaattatac tcttttgtaa atcataataa atgtttatat aacaatttac
     1261 tttctctatc tttgagtact attgaatatt atatattaaa atgattataa ttaatagtgc
     1321 aaaacctata tttcatatcc atattttgca aaaaattcat ttctaaaatc aagtaatcta
     1381 tcttccatta tcgcttgtct tatttgtttc attaaattta ataaaaagtg aagattatga
     1441 gtagttatta atcttgcacc cagtatttca tttgctttta taaggtgtct tatataagct
     1501 cttgaatagt ttttacaagc ataacaatca cattcagggt caagtggagt aaagtcttca
     1561 gcataagttg catttctaac tactacttta ccttggctag tcattgcagt tccatttcta
     1621 gcgatacgag taggtaggac acaatcaaac atgtctactc ctcttataac accttccact
     1681 aagtcatctg gactaccaac tcccattagg tatcttggtt tatcctgtgg cataagtgga
     1741 gttgtatgct ctagcacgtc atacataagt ggttttggtt ctccaacact aagcccacca
     1801 atagcatatc ctggtaaatc aatactagtt atttcttttg ctgattgttc tcttaagtct
     1861 ttatacatcc caccttgtat tataccaaat aaagcttgtt tgtcggtatt attatgggca
     1921 tctttacatc tttttaacca tcttgttgtt ctttctaaag aattttttac atattctcta
     1981 tctgatgggt atggtgcaca ctcatcaaac gccatcatta tgtctgaacc caatgcattt
     2041 tgtatttcca tagctttttc tggagttaga aaatgttttg aaccatctaa gtgtgaccta
     2101 aattctacac cttcttcttt tatctttctt aaaggcccta agctaaatac ttggaaccct
     2161 ccactatctg taagtattgg tttatcccag ttcataaact tatgaagacc accagctctt
     2221 tttataagtt catgacctgg tctcatatat aaatggtatg tgttactaag tattatttgt
     2281 gaaccaatct cttttaattc ttctggtgtc atagatttta cagttgcttg agttccaact
     2341 ggcatgaata ttggtgtttc aataatccca tgtggagtgt gtaatcttcc taaccttgca
     2401 ccactctgct tacaagtctt tatcaattca tatcttactg cgtacataaa gttctcctta
     2461 accatattta tttctcatta tttcttaaga tatatttgta ataagctatt catataattt
     2521 attgtctaaa tagatttatc ttcgtcttct ttgttctgtt gctccttaat tgcttcttca
     2581 tcatgaatac tcattatatt aagagcactc ttgtcttttg ttactaaaaa cttttttata
     2641 gcaaatagtc ctgctatagc tataactcct attaaaacat ctatcatacc ttcattttta
     2701 ggcagtagca acatttttct agctattgcg taaagtaatg cctcaataaa tgctcctggt
     2761 atatgaagag atagcattac tacaagttca acacctatta ctaaaagtaa tatttgtgct
     2821 aaaaaatcat taagctgact gtattgtact ggattttgaa aatcaactat atatgctccc
     2881 cacataagtc ttagaacatc tatagttcct aaaaatactg ctattagaac tactactgct
     2941 aaaacacgtt caaatataaa agcaatcttt aatatagggt tattcttttt ttgtttttgt
     3001 aaactatgca ttcattacct ccctacttta taatcatagc atctccaaaa ctgaagaatc
     3061 tatatctttc ttttactgcc tcattatatg catttaaaac attatccttt ccacaaatag
     3121 cacttactaa cattattagt gtagattctg gtaaatgaaa attagtaata agtttatcta
     3181 aaactttaaa tttataacct ggatatataa atatttctgt ccatccacta gtttctttca
     3241 tctttccatc ctcattacaa gctgattcta tagttctaca actagtagta ccaacacata
     3301 ttacattgtt tccattttcc ttcgctctat ttattttgtc agctgcttct tggctaacca
     3361 tataatattc tgaatgcatc ttgtgattta aaacatcttc tacttttact ggtctaaaag
     3421 tgcctaaacc aacgtgtaga gttacaaaag ctatatctac acctttttct tttattttat
     3481 ttaacaattc ttctgtaaaa tgtaatcctg ctgtaggtgc tgctgctgaa ccattatgct
     3541 ttgaataaac agtctggtat ctttcttttt catctagtct agcagtgata tatggaggta
     3601 gtggcatatt tccaagttca tccaatattt cttcaaaaat tccatcataa aggaatttta
     3661 taattcttga accttcatct gataaatcta caacttctcc aattagcttt ccatttccaa
     3721 aagaaaattt agtccctact tttgctcttt ttcctggttt tactaaagcc tgccaagtat
     3781 cttcctctgt tctttttaat aataaaaact ctattttacc tccagtttca agcttctctc
     3841 ctattaatct agctggtata actctagtat tatttaatac taaacaatct cctggattta
     3901 aatattctat tatatctcta aatactttat gttcaatatt acctgtctct ttatctaata
     3961 ccatcaatct tgagctagct ctatcttcta taggaacttg agctataagt tcctgtggta
     4021 agtcaaattt aaaatcactt gttttcaatg ttaaacttcc tctcttaaaa tttctccagc
     4081 tacaataata tcaaaaaaac aacttatttt ccacaatatt accataatct atcaagaaat
     4141 tgctattttt aacagacacc atatccacaa tatatagata tttttccacg atatacatgt
     4201 cttatccaca atatatagat acaatacaca caatttttat cgtgtttatc cacagtatct
     4261 acagatataa ttaccatttt gtggatatat ttgaattttt agccatttta gaaatatttt
     4321 aatttaatga ctcttttatt ttataaatac tcgtataatc tatttttatt tttcattagg
     4381 atatggaatt tttaagtgtt catatgctaa tggcatggct actcttcctc taggacctct
     4441 atttataaat cctaattgaa gcaaatatgg ctcatataca tcttctattg tatttctatc
     4501 ttctccaata gaagctgcca atgtatctaa acccactggt cctcctctaa atttttctat
     4561 tatagtcata agaagcttct catctacaaa atcgagaccc aaactatcga ctcctaaaag
     4621 ttccagagca tcttttgcca ccttatcagt tatttttcca tcagctctga cttgtgcaaa
     4681 atctctaact ctttttagta atctatttgc tatccttgga gtccctcttg aacgttttgc
     4741 aagttctaat gctccattag actgtatctc tgcatctaag atgcttgaag acctaagtac
     4801 tatttttgaa agttcatcaa ctgtataata atccaattta caaataacac caaatctatc
     4861 tctaagtgga tttgtaagca taccagctct tgtagtagct ccaataagtg taaatttagg
     4921 aaggtcaagt cttatgcttc tagcagatgg ccctttccct attattatat ctaggcaaaa
     4981 atcctccatt gctggatata aaacttcttc aacacttcta ttgattctat gaatctcatc
     5041 tataaataaa acatcatttt catttaaatt cgtaagtatg gctgccaaat ctccagctct
     5101 ttctattgcg ggtcctgacg taattcttaa atttaccccc atctcatttg ctattatact
     5161 tgctagtgta gtttttccaa gacctggtgg tccatataaa agcacatggt ctaactgctc
     5221 atttctcgat ttagctgctt ctataaatat actaagttgt tctttggatt tttcttgacc
     5281 caaataatct tctaaagttt ttggtcttaa actgttttct atatctatat cttccatctt
     5341 cattgtagaa gttattatcc tattttcatc ttcaaaacct tgcatttata cccctcctct
     5401 cgtacttttg aaaatctact ttatatattt tcaaatttac ataaactcct taaatattgt
     5461 tttaaaatcc aaatattata aaatccaaat actatttcaa ggatttactc attatatatg
     5521 taagagcttt ctttataata gcttctgtat tcataccatc ttttttacat ttttgaactg
     5581 cctcttttga ttccgacaaa gtatagccta aagctacaag tgcatctaca gactcatctt
     5641 gtgatatcaa ggtaggtttt tgtgataata acgttggttc aaactctatg ttatttttgt
     5701 ctaccttatc cttaagctct aagacgattc tttctgctgt ctttttacca acaccaggag
     5761 ctttggataa ttttccaatg tcttcactta aaatatatgc tcctaactgt gctgatgatg
     5821 caaatgatag tattgaaagc gccaccttag gtcctatttt agatacagat gttagtaatt
     5881 caaacatctt aagttcttcc ctacttacaa aaccacaaag actcatatca tcttctctta
     5941 caattaattt agtatatatt tttgtggctt ctcccacttg taaattcatt attgtattgg
     6001 atgatacatt tattttatat cctatcccat tattttcaac aactatactg tctatataaa
     6061 tttcttctac tgtacctttt atataactat acatacttac cacctatatt ctttagagtt
     6121 ttttcaagtt tgtttgaatg agcatggcaa atagccacag ctaaagcatc tgcaacatca
     6181 tctggttttg gaactttttt tagacttaaa aatgaagtta ccatctgttg aacttgtgcc
     6241 ttatctgctc tcccatatcc aactacacct tgttttactt gaagtggagt gtattcatat
     6301 acaggcttcc cattatgagc acatgcaagc atagtaacac ctctagcttg tgcaactgtt
     6361 atagctgttt ttacattctt gttaaagaat aattcttcca ttccaacttc atctatattg
     6421 taattcttta ctattgtatc aattccttta tacacaagtt ccaatcttct cgatatattc
     6481 atatgggcag gtgttgtaac tgctccataa tcgattactt taaacttgct atttttgtat
     6541 tcaattatac catatccaac tatggctata cctgggtcaa tccccaatat tatcattttt
     6601 taaaattccc cctaaaaata aaagaaatac caaaatgtat ttctcataaa aacttatgtt
     6661 ctaaatcatt atatcataaa taatattaac aaatcataaa atgctctatg taaaatataa
     6721 aatcaaataa aaaagttata tcaaagcaag attttacctc aatataactt ttatatttat
     6781 tacaaataaa ttattgtatt tttataggaa ttaaatgtga ctacaatttt aatactaatc
     6841 tatttaatat taaattattt attattaact tacttagtat ttaacttgaa ataatattaa
     6901 tttactatta acttgcaaag tcttctaatc ttgaataggc ctcttcttca gtatccataa
     6961 ctacaccttt tttgatttca tcttgaactg ttttaggaag tgaatctact cttatttttg
     7021 ttttttctac taatgtttta cttccactat catcatattt atatagtgct atatttccat
     7081 tatcattttc aatagtatat ttacctttta tagtagcgtt cattgcttgt acatctttat
     7141 tatcactata tttagattct accaatatga tttcattttt atccatactc tttatttttt
     7201 tagttggata ttctttttta aaatatgata ctatttctga ttttgtctta tctaataatg
     7261 actctggaac tatcccagcc attgtaagtg ttctcttata gttagtttca ttttcgcctt
     7321 cactttgaag ccaaacttca caatttttat ttaatccaaa agcttctttt gacttttcgt
     7381 ccatattcgc actagcactt acaactttac tagtttgaac tccattattt tttaatcttg
     7441 tccctatata aactccgcta cttaatacaa atactccaac cagtattaag gcagatattt
     7501 tccaaggaaa taattctttt ttcttctttt caaacattga ttacgcctcc tttttctatt
     7561 attttctcca agctgtaagt ttttatacaa tttaggtaaa ttttagaaga aaatatattt
     7621 atatttcaaa ttactctaag aatagatatt ttctaaaagg tagttttctc ttaatggtta
     7681 tattcttgta attatatgga cataatttat aataaataat cgtttaggag gtaataactt
     7741 tggaaagaat aaactgtaat gtaattaact gttcacataa caacagccat atatgttatg
     7801 cagataaaat ttttataaat ggtgaaaaag ctcacaatga cagacatact tgttgtagtt
     7861 catttttaga tgaatctatt tatagtaatt tatctagcaa cgttaataat agaaattgtg
     7921 aggaattagt ttgtactgta aaaaattgta aatacaacaa agatggtagc ttatgtacac
     7981 ttgataatat attggttgct cctgaaactg atagagtaaa cctttatatt gaaacatgtt
     8041 gttctagttt cgaatgtaaa tagtcatagt ataaatataa gggtgtcaaa tgtaattaaa
     8101 ctaacatagt gtagagtatc tacaactatt agtttgatta tacttgacac ccttatttat
     8161 tttaaataaa atttcattat tataaccatg tttataatgt tctaactgta attaaatttg
     8221 ataaatttct ataaattggt ataatcaata tgtaaattga atttagcaaa ttaatcataa
     8281 aaataaaata tcgagtatat agtgttatac taactagaaa tgaggtgctt atgagtacat
     8341 tttatattca atttctttat tttgtaattt atagctttat aggatggtgt tgtgaaacta
     8401 catattgttc agttttacaa aaacaatttg taaatagagg ttttttaaat ggtccatttt
     8461 gccctatata tggatttgga gcattagcaa tagttgctac cttgactcct tttgtacaca
     8521 atattccttt gttatttttg tttagtataa ttataactag cataatggag tattgtacga
     8581 gctttatttt agaaaaaata tttaatatga cttggtggga ttattctaaa cataaattta
     8641 atattcatgg aagagtttgt ctggaaaact cacttatgtt tggtgtatta tcattgattg
     8701 taatgcttat agtacatcct attgtggttg actttattaa ctctatctct aaaaatgtgc
     8761 tatttatatt tgctatatct atagaaattt attttgtttt agatttagtt atcacagtcc
     8821 atacaatttt acaattaaat ggtaagttaa aacaaatcaa tcttataatt aaggaattaa
     8881 aagataagaa agaatactac aaacttatta cacaagaaac tatagaaaac aaacttgaat
     8941 ctttagttga aaatagactg gatactttgg aagatagatt tgaaatactt aaggaaaaag
     9001 aaaactatcg ctatgctaaa aatcgtattg atgagctaaa aaataaactt aatactttgc
     9061 tgtctaacca caaattactt catagacgta taatcaaagc attccccaac ataagctcta
     9121 ataagcatac agatatttta aataaaatca aagaaaatat taaatcaatt aaaaaagata
     9181 aataataaaa taaacacata agttaaataa taaaaatact aatacataag attaaggttt
     9241 agaatatttt ttgtaaaata ctctaaaccc taattaatat actattattt ttaatcttaa
     9301 tttataattt tagtttattt tacttttaac acagtaaatt taagcgttta aacttcaata
     9361 cactaatata ctaccccatc aatatctcaa ctgattctct aagtgtgcta atctctccca
     9421 catttccagg gaaaattata tatggcatat taggaaattt gctttctttg cctgtcatcc
     9481 atactggtat accaggtttt atctgtccca ttactgtagc tttttttact cttagtgctt
     9541 tagtaccaac atcactagaa gtaattccac ccttagctat aataaaattt ggtcttacat
     9601 taagcatacc aattatactg gtaactgcat ctgatatttc tacagaaatc ataagctgtt
     9661 tatctttatc attcgtatcc aagtcaaaac gctctcgtct agtataaaca gcaacagttt
     9721 taccactgct tattttttcc tctacaactt ttataactct ttttacctca ttttttagac
     9781 cattttcctg aagaactaaa tgttgattaa attctataaa ttctattgga tatttacaat
     9841 ttttaagttc ttctaactgt tgggtagtct tattcacatg tgaaccaaca agtataattc
     9901 ctccattggt attacctttt gatactaact catccttagt aagcatttcc ttatcactca
     9961 ctccgccaag tacttttgtt atggcagcag cgcttctaaa cataaattcc ttaccactat
    10021 ttactgctcg tataaacgca attgtaaata cctttacatc tacataatcg gttgcattta
    10081 caataatttt attgaatcct tcagcttttt taagtttttc tgttatagct tcaatattta
    10141 gctctctaag ttctttagaa gaaatatata tcatatcact agatttgtac tttccatgtg
    10201 tcttttcttc acaccactca cctatatcag atgatttata tccaaaagat ttatcctttg
    10261 caaattcagt cattcctgct ggtacaagta tttctccttc tttaacataa tgtacattat
    10321 ctatagtaaa cctaccacct tctttaaaaa atggcattat tatttcaccc tgaaataact
    10381 tccccgataa tctctctact tcgcttttta ataaattagt ttcaacagga taatgtcctc
    10441 tcatagtaga atcactacga cttatgatta taaaatcctt attaactttc tttgatacat
    10501 ccacaatatt ctttgaaatt tctttatgtg cttttgtagt ttgagcaaca gtaaatcccc
    10561 ttgaattagt aagtataaaa aacatactat ttttctcatt aaaaccttgt tccatactat
    10621 ccttatccca atcagtataa acagaaatat catgaactgt ttgtacacct gttgggtcat
    10681 catctaatac aacaattttt ctatctaaat gcttaagctc atctgcaaga agttcattta
    10741 atctgtcctc atctacttgt ttaaaagtat taagtatatc tatgctaatt ggttttactt
    10801 ctctcattta aaacgcccct ttatgtaatt tgtatagtct aaatgtaatt tatataatat
    10861 aaacaatagt ttgttattta tagacctgga agtggtataa ttccatttat aacactaagc
    10921 aataaaacca ttataaatgc tacaactcca gcaatggttc ctccaattgt ccaagagttt
    10981 aaagtatctt taactgataa tccactaaat cttgaaagta cccagaatcc agaatcattt
    11041 ggaagggaga aaccaactcc tccagcacat atagcaagcc ctactaaaac tggtgacatt
    11101 ccagttgcca aaattgtagg tcctaaaatt gatgatgttg ttactaaggc tacagtagca
    11161 gaaccttgag aaattctaag caaagcactt aataagaatc caagcactac aactgatata
    11221 ctaagaccag acatagtatc aactaagaag ttacctatac cacttgcatt tataacactg
    11281 ccaaacgcac cacctgaacc tgttatcaat aatattagcc ctgctgaatc agcagcttca
    11341 ataactacat ctcccatagg tttatttatg tatctcttta aaaatcctat tgccaccaat
    11401 attccaagaa gtattgctat gtttttatct cctataaatg caagtactcc actcattgta
    11461 gaaccttttt ctaagaacat actaaataca tttgatataa gtataagaag tattgggaac
    11521 aataaagaaa acatagaaat tccaaaacct ggtactttag aagaatcatc atcttcaatt
    11581 tttatgctat cttttccctt ttctaaattt ttttctctat cattctcatc aatagcataa
    11641 gctggatatt tttcaaaacg ttttccataa atccatccac ctataagaac tgctggaaaa
    11701 gcaacaatta tagaataaag tataaaacta cctacatttg catttacatt tgctgcaact
    11761 gctaaaggtc ctggtgtagg tattacaagt gcatgaccta ctatagtccc aactcctaag
    11821 gcacatacaa aaaccattag agatttctta gttactctcg aaacatactt aattataggc
    11881 atcattataa caaaagctgc atccataaaa actggaattg aaattaaaaa acctgctgta
    11941 gttactgcta aagcactgtt tttttctcca gtcttagcta acaacccctt tgcaatagat
    12001 tctgttgctc ttgattctga aagaatattt ccaaatatta ttccaagtcc aattacaata
    12061 cctatactac ctaatgtatt tccaaatcca gtcgatattg tggttgatat ttcatttata
    12121 ggcattctaa ccataaagcc tgtaattata gatgttatca gtaatgccaa aaacggattt
    12181 actttaaatt taataatcga tacaagtaaa attcctatag atataacaaa tgctatcaaa
    12241 agtccatatc ctgtaatcat aatctttacc cctttatcat tctactttat tatttatcaa
    12301 ttttattttt cttttactat gataacttaa cctcgctgta tttatataat ctaatcctaa
    12361 taatatgtat ttaattatat tgatatattt aaatattaat tattgtgttt ttttatttca
    12421 acacctgcca acttttcaaa atactgtgct attccactat ggtcatcttc caaatgtcca
    12481 tgtactttta atgtctgcat aatttctaat aattgacttg tcataggtag cggtacatct
    12541 aaattgtgtg ctgtttgcat tacattctta atatctttca aattaataga tatttttcca
    12601 cctggtttaa aatcacgatt cataatcatt ggtatcttcg catccaatat tgtactacca
    12661 gctaggcctc ctcttattgc cttatatact ttttctgggt ctgcacctgc ttttgctgca
    12721 agtacaaatg cctcagaaac tgctgctatc gtcaaattaa ctattacttg gtttgtaagt
    12781 tttgtaacac ttccactacc attatctcca attaaaatag atgagcttcc cattatatta
    12841 aaaaatggct ctactttctt ataaatttct tccttaccac cagccataaa agcaagtgta
    12901 ccatcaatcg cttttggctc ccctccactt actggtgagt ctataaattc taagcccata
    12961 tcttttaatt tatttgcaca caatattgat tctgtaggag taactgaact catatcgact
    13021 actatacttc cttcttttaa agtttttgcc aatccatcca tcccaaataa tatatcctga
    13081 acaatagttc catttggaag gattgtaaat acaatatcac attcttgacc catttctaaa
    13141 acacttgcgt attttgctcc acatgatata agttcatcca cagcagattt attaatgtca
    13201 tacaccaaaa gattacatcc atcttttaat aaattttttg ccattggctt tcccattatt
    13261 cctaatccaa taaatcccaa tttcatttta taaccctcca tatcttcaca tttttgaaaa
    13321 cgttttttat gttttagagt atactacata ccagtatacc ggtcaatata aaatataaaa
    13381 gtatttattt taacaataaa tagtaatttt taaagtcatt tattatattt agtcatccta
    13441 tataagtttt aatacatact attgttttta aatataatat gctactttta cagttttgta
    13501 tttttactaa tttcaattag ttttaattaa tctgccttta atgttaatca tacttcacac
    13561 ttcaataatt ttttatattt tttaatctta tctgcaattt aaattgcaaa agattgacct
    13621 tattttcact atgttgtatg cttaaaatta tataagtttt tgtattttta tattgtttag
    13681 gggggtatta ttaagtatgg ctacatttaa aagattacaa gaacaagcat atgactactt
    13741 aaaagaattg atactatctg gagaaatgat agaaaatgaa atatattctg aaacaaaact
    13801 tgctagtgaa ataggtatct ctagaacacc tataagagat gctcttcaaa gacttagtca
    13861 agatggattt atagatataa tcccaagcaa aggatttaga atccatcaaa ttacagcaaa
    13921 tgaaattgta gagatattcc aaatacgctc tgcaatagaa gggttttgta cttttttaat
    13981 tacaagtcaa tacaaagaag ctagggcagt agaaactata agtaaattaa aacatctttt
    14041 agataaacaa aaagatattt tactaggtga taaaaattta aactcatttg cagaatacga
    14101 tacattattt cacacaacta tagtttcata tgctcaaaat actgaatttg acaaaatgtt
    14161 taacaactat atgtacagaa taaaaaaatt agctctcgat tcattatcac atgaaggaag
    14221 gcttgaaaca acattaaaag aacacacaga tatttttaat aatattgcaa atggatgtat
    14281 tgaagatgtt tacaaaacta ctctcattca tatggaaact cctaaataca ttaatttaga
    14341 agatttttgt aacaataagt gattttagta ttatactttc atttattaaa aatatatcta
    14401 cctcaaatta atttcctaat ttataaaaaa tattctataa ttatattgta actaatacta
    14461 ttctaaggag tgattttttt gaacgaaaat gttgtaacta atgaaactat tgacaaagat
    14521 tatgctattg aagaagttcg tatggcatgc aaacattttg gtgacttata tttttatttt
    14581 tccaaagtgt tatttgaaga gtttggtgaa gataaaacta tcgaaatact aagaaaagtt
    14641 ttatttgaaa ggtctgaaga aagagctatt gctatgagag aaagagctct tgaaaatgga
    14701 gatgaattaa ttgcagacaa tattattagc acaactgatg ttccatttct aggatgggtt
    14761 cctgaattta aagagttaca ttgcccttat ggagcttcct ggcttttaag atttgaggaa
    14821 aacccttggt ttaaaaaatt cgcttctctt tactgtgatg ttacagatac tactgtagct
    14881 gaagtattta caggagatac gtcccataaa ataacaaaaa acattctatg gggagataaa
    14941 agttgtgaaa gaatatattt ccatgatgac aaggtatctg aaggtaaata tacttatgga
    15001 aataaaaact gtaaa
//
LOCUS       DAEPOG010000099        14647 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEPOG010000099 DAEPOG010000000
VERSION     DAEPOG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.099"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..709
                     /locus_tag="KR432_002395"
     CDS             <1..709
                     /locus_tag="KR432_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBH0640925.1"
                     /translation="LSKFNLKHKYICHTSGSLKSSILFCSKKAGALIYSIHPIFAFSN
                     KNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDTSSTYHLANVLVSNL
                     VLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTKALTGPVSRGDITPI
                     RKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIENAIENLISKSEKYKE
                     IYKLLGGKNDEKYHTDF"
     gene            684..1511
                     /gene="panB"
                     /locus_tag="KR432_002396"
     CDS             684..1511
                     /gene="panB"
                     /locus_tag="KR432_002396"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH0640926.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1530..2378
                     /locus_tag="KR432_002397"
     CDS             1530..2378
                     /locus_tag="KR432_002397"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH0640927.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            2789..2971
                     /locus_tag="KR432_002398"
     CDS             2789..2971
                     /locus_tag="KR432_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640928.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            complement(3259..3461)
                     /locus_tag="KR432_002399"
                     /pseudo
     CDS             complement(3259..3461)
                     /locus_tag="KR432_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003381593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            3803..4717
                     /locus_tag="KR432_002400"
     CDS             3803..4717
                     /locus_tag="KR432_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBH0640929.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5107..5754
                     /locus_tag="KR432_002401"
     CDS             5107..5754
                     /locus_tag="KR432_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0640930.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(5921..6490)
                     /locus_tag="KR432_002402"
     CDS             complement(5921..6490)
                     /locus_tag="KR432_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640931.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(6495..7394)
                     /locus_tag="KR432_002403"
     CDS             complement(6495..7394)
                     /locus_tag="KR432_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0640932.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(7405..7554)
                     /locus_tag="KR432_002404"
     CDS             complement(7405..7554)
                     /locus_tag="KR432_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640933.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(7547..8131)
                     /locus_tag="KR432_002405"
     CDS             complement(7547..8131)
                     /locus_tag="KR432_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0640934.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
     gene            8552..9877
                     /gene="cdeA"
                     /locus_tag="KR432_002406"
     CDS             8552..9877
                     /gene="cdeA"
                     /locus_tag="KR432_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBH0640935.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(9972..10766)
                     /locus_tag="KR432_002407"
     CDS             complement(9972..10766)
                     /locus_tag="KR432_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640936.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(10767..11642)
                     /locus_tag="KR432_002408"
     CDS             complement(10767..11642)
                     /locus_tag="KR432_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640937.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(11646..12014)
                     /locus_tag="KR432_002409"
     CDS             complement(11646..12014)
                     /locus_tag="KR432_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0640938.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(12197..12856)
                     /gene="deoC"
                     /locus_tag="KR432_002410"
     CDS             complement(12197..12856)
                     /gene="deoC"
                     /locus_tag="KR432_002410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH0640939.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(12892..14436)
                     /gene="murJ"
                     /locus_tag="KR432_002411"
     CDS             complement(12892..14436)
                     /gene="murJ"
                     /locus_tag="KR432_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0640940.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
BASE COUNT         5283 a         2180 c         1751 g         5433 t
ORIGIN      
        1 actttccaaa tttaatttaa aacataaata catttgccac acaagtggct ctttaaaatc
       61 tagtatattg ttctgttcaa aaaaagcagg tgcattaatt tattctatcc atcctatatt
      121 tgcattttcc aataaaaata ccaacataaa aaaaatgaaa gacatttgtt tttccataga
      181 aggtgataat gaacaagatg atttagttat tcaaaatttc atagatggtt tgggaaacca
      241 attttttata agagataagg atacttcatc cacttatcat ctagctaatg ttttagtttc
      301 aaatttagta ttatctttac tagatatagg agtaagttac tttattaaat taggtttatc
      361 tgaagaggaa tctttaaaag ctatcagtcc tttagtaaaa aaaaatatag aaaatatact
      421 tgataatggc tttactaaag ctcttactgg tcctgtttct cgtggagata taacaccaat
      481 aaggaaacat ctatctgtat tgaataaaaa agatgaggat atctacaaaa tactatcctt
      541 aaacctacta aaaataattg ccttaggaaa tgacaattca ttaaaaggta caaaaaatat
      601 tactatagaa aatgcaatcg aaaatttaat aagtaaatct gaaaaataca aagaaattta
      661 caaactttta ggaggaaaaa acgatgaaaa ataccataca gacttttaag aacgctaaat
      721 atgaaggaaa aaaactatct atgcttactg cttatgacta ctcaattgca aaaataatgg
      781 atgaatgtga tataaatggt attttaatag gcgattcatt aggaatggtc ataaaaggtg
      841 aagaaaacac tctatctgtt actatagacg aaattatata tcatacaaaa gctgtaaaaa
      901 atggtgcaaa aaatgcctta atcgtaagtg atatgccttt tctatcttac catgtttcta
      961 tagaagatgc agttaaaaac gctggtagac ttataaaaga aggaggagct catgctgtaa
     1021 agttagaagg tggctcaaac gttataaagc aaatagaaag cattgtaaat gctcagatac
     1081 ctgtaatggg gcatttagga ttaactcctc aatcagtaaa ttcatttggt gggtttaagg
     1141 ttcaaggaaa taccagtgaa acagcaagac aacttattga agatgctaaa ttaatagaaa
     1201 aagcaggtgc attctctatt gtccttgaag gagttcctac gaaaatagct gatatggtaa
     1261 ctaattctat ttcaattcct acaataggta taggggcagg aattaactgc gatggacaaa
     1321 ttttagttta tcaagatatg ttgggagtgt ttggagattt tgttcctaag tttgtaaagc
     1381 agtatgcaaa tataggagat ataatgaaag attcaattaa aaattacatc ctagaagtta
     1441 atacaggtgc atttccacaa gaaaaacaca gcttttcaat aaatgaatct gagttagaaa
     1501 aattatacta atattaagga tggtacaaaa tgcttgtaaa agaaattaaa ttactaagaa
     1561 atataatcaa agattggaga aaacatggct attctatagg gcttgttaca acaatgggat
     1621 ttttacatga aggacaccaa agtcttataa aaaaagctgt aaaagaaaat gataaagtag
     1681 tagtgagtgt atttgtaaat ccaactcaat ttggaccaaa tgaagatttt aatagctatc
     1741 caagagatat agataaagat tttaaatatt gtatggatag tggagcaaca gtagtcttta
     1801 acccatcacc agaagaaatg tatttaaaag gtaattgtac taccattaat gtttctggac
     1861 ttacagattt tctttgtgga gctaaaagac ctgttcattt tggaggtgtc tgcctagttg
     1921 tttcaaagtt tctaaatata gtaacacctg ataaagctta ttttggagag aaggatgccc
     1981 aacaattggc tgtaattaaa agaatggtca aggatttaaa cataaataca gaaataatag
     2041 gctgtcctat aattcgtgaa aatgatggat tagcaaaaag ttcgagaaat acatatctct
     2101 ctgaggaaga acgaaaatct gctctaatat taaataagag cttaagttta gcaaaagaag
     2161 aattagtaaa aggcaatcta aatccaaaga atataaaaga attaataaca gctaaaataa
     2221 attctgaaca cttagcaaaa atagactatg ttgaaattgt agatagtgag acattacaac
     2281 ctgttaagca aatagaacat tctatccttg tggcaattgc tgtgtttata ggaaaaacca
     2341 gactaattga taattttaca tttgagttaa atatatagta tataatttta tataatatat
     2401 ctaaaaaata atagaatacc taaaaagtaa taaattacct aaaaaataat aaattgctta
     2461 gaaaataata gattacaata actaaaattt taaaattaaa ttccttaaaa gtatgattaa
     2521 ttataattat ataagtatat tcttattaca cttatattaa tttatattaa atatctacta
     2581 actcaaatat tcataattaa actttaaact ggataaattt tcttttaaga atgaaagttt
     2641 atccagtttt gcgattataa taaccagttt tacatatcca cttgtaaaat atatctaagc
     2701 aatatatcat actatataga tttatgagtg aaataatatc tgctatttac tgaatatttt
     2761 tatttttcgt tttttagggg gattagttgt gtttaaagat aagattgatg aatgtgttca
     2821 tataatgact gcatatattg ctagtttaaa agaatactat tcattcatag aaactcaaat
     2881 tggtgatttt attaaaaaat atggagaaga tgttgtagag ttatgtctcc atagagtgat
     2941 gattctacta tgcgaatgtg gtttggcata aaattaaatt tattaaaatg ggtaagaaga
     3001 atcctatata atattcttct tacccatttt aatatatcaa tttcatagct tatcgtttaa
     3061 aatattttac ctccattact agacaacttt acattctctt tttaattcta gtatatttaa
     3121 atcattttag ttgctcctta tttattctta ttacttgttt tattttataa ataaaataat
     3181 tctttttaat aatgtttttt gttgatattc ctatcacaca tccaattatt tatcattgtc
     3241 ttgaaattgt ttgcatactt aatttaatcc aattgaaaca ccccagcata gaatcccctg
     3301 taaaatagaa gttggattaa atcctaacat ccatacagaa aatactactc caagcacaag
     3361 caatataatg gaatatattt atcacttaat tttttatact ttttaaatcc catacctaaa
     3421 acattaagtg cagctattag aattaataat tgttgtggta taaaattatg attgcttcat
     3481 atttattcat tttttgctat aaaagttctt gtttcacaaa aaatatttgt tttgtatcaa
     3541 aataatactt ataaattaca taattctcat aaaaatttct agtattactt gtgctcttaa
     3601 taaataatag ccaacaagta ccttaaatca ttatatctat atctaataag aaacacagta
     3661 agataatttt ttcgcccata acttaataaa aactgttgat attagtagca attgaattta
     3721 gttaatttat ttataatata cataaattta aatattacga ataaagtatt ataaatagga
     3781 tactttctaa agggaggaaa ttatgataga taatcaaaaa tatgttattt tatcactaga
     3841 attacattta tttttttcaa gaattatgaa agagcatgct ctttttttag aagcaggatt
     3901 cacaaataaa aattataatc ttgctatgga agctgaccac tataaaaagc aatttgaaga
     3961 tttattatca tacactgtta gtgctagtaa tggtataatt agacctgata tattatattc
     4021 agaagagctt gtaactactc tcacatcagt tgcagaacaa aaaacagaag agtttacagg
     4081 gatagaaata aacaaagaca tcactacaag agaattaaat ctacaaagtg gtgtaaaccc
     4141 acaagttggt caagacttag tgaactatgt agctcaactt aactctgatg caataagatt
     4201 acttgatggg cttattaatt ttaaagaaag agtcttagat ggtgtactat catgtactat
     4261 atttacatca aactaccctc tacttcttga acatataata catgaagcaa atttatatcg
     4321 ttcttatgta gttgaccttg aaaataaaat agatattgag tcaaaaaacg ctaaagaaat
     4381 agaattattc tgggaccata ttatgatgga acatgctctg tttatgagag gattactaga
     4441 cccctcagaa ggtgaactaa taaatacttc aaatgatttt gctataaaat ttaatgaatt
     4501 aattgaaaaa acaaacgaaa tgactgattc taatatcaag aacattacag aagaaactct
     4561 aaatgaaact gttgagttta aagattttaa agaagcagga gcatcaggaa tagaacagtg
     4621 taagataaaa tctataatat taccactttt agcagaccat gttttaagag aggcaaatca
     4681 ttatattaga atattggaga gttataaaaa catgtaatta aaaattactt ttaaaataat
     4741 ctttgcatat ttagataaaa taaaggtaca gttaactgta cctttatttt ttataaactt
     4801 ataaaattta aacaagttat tcataaaata aaataatcat catctataac ccacaacttt
     4861 attttatatt aaataaaaag tattagtgat tatgttttat aagtatttct ttattcagtt
     4921 atttgttaaa aactaatagt aaatcttata ctaatcgcat atttatgtta taactatatt
     4981 gtaactattt tcgtaatctt aggaaaagat tacttataaa attaacctta tacaataata
     5041 tatcgtataa tcttaattag cataatagtt acaatattaa aatttaaata tgggagtgat
     5101 tttagtatga taagacaaac accattagag aaacgatatg acaagctagg tcctcatatt
     5161 gtaacagcat tcaaaaaacg ttattttgat gcttattact gtaaaactag aaatgaagct
     5221 ttgcaacaaa ttttagattt aattcctcaa aatcatgtag tatcatgggg aggatctgaa
     5281 acacttaaag aaatgggcgt acaaactgct gttagagaga aaggatttaa agttattgat
     5341 agagatttag caaaaactcc agaagaaaga actgaaatta tgcgtcaagc acttctatgc
     5401 gatacttttt tgatgagtag caatgcaatt agtgaagatg gacaactctt taatattgat
     5461 ggaaatggca atcgtgtagc tgcaatgata tttggtccaa aaaatgttat agttgtagct
     5521 ggtatgaata aaattgtaaa aaacattgat gatgcaatac aaagaactag aacaattgca
     5581 tctccagcag ttgttcagcg ttttcaagat gtcaatacac catgctatat taatggaagt
     5641 tgtatagatt gcacaagtcc tgaaagcatt tgtgcataca tggttactac aagaatcagt
     5701 cgtccagcaa aaaaaataaa ggtaatattg gtaggagaaa atcttggtct ataaaatata
     5761 catacatgta aatagctcta taaataataa aatcatatga aataacttat ttctaaaagt
     5821 ggtagaataa aagcttctac aagactttca ttctaccact tttatattct tttaatttgt
     5881 gtttctatat tattaattgc tccaattttc atatatatta ctaactattt ttattttcat
     5941 aatttttttg ttcttcatca agctgtttat aataatcttt attatagtca gctagataat
     6001 ctataccacc tcctgtaatc acatttaata atttcattgc agattcttga ttaactttac
     6061 aaacataatt atcaccagat ttaacaagct ctaatgtaat attaaattct tttttctttg
     6121 cagaatcaat atctttcata gttggatttg gcatcatttc tcctagttta ggaggattct
     6181 tttttaaaat agaaaccata tcagtattgt taatttttaa atctacagtc actttatcct
     6241 tattattctt tatatctgaa atttcccaag tcatattttt agaatacatt ttaaatcctt
     6301 ctttatcttc gtatataaga ttacctaacc ctgattttgg gaaatactta tcaacagtat
     6361 ctgcatcttg tgtttttatt gaattaaaca tactatccac ttcatttttt atcttattat
     6421 cattagtaca accagctaac accagtactg atattgcaaa cattataata catagaataa
     6481 atttcttcat taaattatat ccccctatac ttttttgcac aaatattatt ctctttttta
     6541 tcttttgaaa aagttgtagt aattgcattt tgtggacaaa cttttataca ttcaccacac
     6601 ctaatacagt ctggactatt tacatcttta tatattggta tatcaagctt acattttgtt
     6661 tgacatgctg tacaatttgt acatttatct ttatcaattt taaatctata tattgaaata
     6721 ggattaaaaa aaccatagat tgctcctaaa gggcatatat acttgcaaaa tggcctataa
     6781 gtaatgattg acatcaataa tatcaatagc aaaatactta acttccagct aaataaaaat
     6841 cctattgatt ctcttaaatt ctcatttcct aaaataagtg gtattcctcc caaaagtgtt
     6901 cctgaagggc aaatccattt acaaaaccaa ggatttccac cccctgctac atttacaata
     6961 gcaactggca aaataattac aaacattatt aagactatat actttagata ttttagatat
     7021 ttatcaccat aaactttttt tatctttact ggaaagggta ctttatgtag taaatcttgt
     7081 acaagtccaa agggacaaag ccaaccacaa acaaatctgc caaaaaaagc accaaatgcc
     7141 attaaaaaac caacaacata aaatgagaat ttatactctc tgcttgataa aactgcttgt
     7201 aatgaaccta ttggacatgc tccaagtgca cctggacatg aataacaatt caatccaggt
     7261 acacataatt ttttactttc tcctccatat atagttcctt ctaaaaatcc atttacatat
     7321 ccatttgtta atgctgtaaa tgcaacttga ataataagtc ttatattatt atttacctta
     7381 gataataact tcattcctct tctcctaacc aataccaata cactcaagac aaatcataat
     7441 tgctttagtt aatactgtat ttgtttctcc acgatatgta cctaatgcaa taaatataat
     7501 actaattgca ataatagaaa tgcctttttt attatcttta ataaagttac tcattggctt
     7561 ttaccatttt tagtctttca tctataattt ttttccactc ttccttacta tttgcaccta
     7621 caaccttctc acccacaaag tttcctttct tgtctacaaa ataagttgtt ggaaactcca
     7681 taactctttt aaaatcagtt tttttaagtt cttcagatac agtcaattgt tggtaagttg
     7741 catctgactt tttcataatg tttttaacta aatttttctc ttcatttgaa agaccagttc
     7801 tcatatcagt tttatcaact tctacaacta atccctttat agctacattg gaattattac
     7861 tctcatattc ttttgataat gcttcaagct ctggcatttc acccacacat ggtccacacc
     7921 aagtagtcca gacattaata agagtaagtt ctttatttga aaatatctct tttgttacct
     7981 tatttccatc aacatcctcc gtatctaaat tactaaacca ttcactatta ctcttgtttt
     8041 tttctactgg cttagattct tttttgctac atccagaaat cacacctaaa acaaaaatac
     8101 atcctaaaac taccataaaa aactttttca tatctcacac tcctagaatt tatatagtat
     8161 ataaaatggc tgcttgaaat aaaaataact tctcttctat attaaaacat tatatttaat
     8221 aaataagaac taatttaaaa taagttgtat aaaagatata tagttaaaat ctatttcttt
     8281 tcagacaaaa tcttatatat atataataac ttataaagct taagattata tacatatatt
     8341 tctaaaaatt ttcttaagaa acagttattc cttactaatg caatatacct atttttacta
     8401 atataagttt attaattagc attatactaa tttatcttaa tatgattttt tgttgatttt
     8461 aagctgttta tcgtataaat aaaatttttc ttgacatata gttactatat agcttattat
     8521 agatagagag taataaaagg aggtaaaatt tatggaaaat ttatttacaa gaaaattcac
     8581 tacttttgaa tttctaaaat ttgtttctcc tgcaattata tccatgatat ttatatcttt
     8641 gtacacaata atagatggca tctttgtatc aacattagtt ggttccgatg ctcttgctag
     8701 tataaatatt gtactaccta taattaacct tgtttgtgga tttggaataa tgatggcaac
     8761 gggtggaggt gctatcgttt ctatacgtat gggtgaaaat agacaggatg aagccaactc
     8821 tacattttct tttatagttt tgttttcatt gattgttggg atttcattca cagtaatctc
     8881 atatttcttt atcaaagaaa tatctatttt gcttggtgca acagataagt tattaccata
     8941 ttgtataact tatggtaaag ttatgatttt atgtacacca ttttatattt taaaatttat
     9001 atttaactac tttgcaagaa ctgatggaaa ttctaaattt agtttatttc tatcagtcat
     9061 tggtggtgta acaaatataa tattggatta tgtatttatt aaatattttg gaatgggtct
     9121 tttaggagct gcagttgcaa ctgctatagg tattatttta acttgtgttt taggtattat
     9181 ttacttctta tctaataaat ctacactcaa attaagaaaa ccaaaaaccg attttagact
     9241 tataagagat actatgataa acggttcttc tgaaatggtt acagaattat ctacaggaat
     9301 tacaacattc ttatttaatg tagtagcttt aaaattagca ggagaaaatg gacttgctgc
     9361 tcttactata gtattgtatg ctcatttttt aatgacatca gtctatctag gattcgctgc
     9421 tggagtgtct ccattaataa gctataattt tggtgctgaa aacagtgata aattaaaaga
     9481 aacatttaaa cattctctaa aatttatatt tgtttcttct cttttagtgt ttattattgc
     9541 tttagtattt gcaccattta ttgttagggt ttttgtaagt ccagataaca cagtatttaa
     9601 actagcctta caaggattaa aaatatttgc atttgctttt ttgtttgttg gtataaatat
     9661 atttgcatca ggatttttta cagcatttca caatggaaaa atttcagcta ttatatcttt
     9721 tagtcgtgcc tttgttttta taatcatagg aatcataatt cttcctccta tgttaaacat
     9781 gactggatta tggcttacag ttccatttgc tgaggttata accatattta tatctattct
     9841 atttataaaa aaatataaag gtagatataa gtattaaaaa ttaatgctta aaatcaacat
     9901 ttattaaaac taataaacct gctttaaaat acaaatttac aaatttgatt tttaagcagg
     9961 ttttatttta gttataataa gtcatttgca tactctttaa ttggtgcttt tctaagtaat
    10021 aaaattataa atattatgca aataactata attcctacct ttgttccaaa taaaataaaa
    10081 ggattctgaa atgaataaat aattttttct aaatacaata gcattttatg catattttta
    10141 gttgcaaaat taactatata aattaccatt aatatcggta atcctatcaa tatagtaata
    10201 ctagtaactt ttctaactct atatgtaaaa gctccaagta atactcctat aaaacctata
    10261 catatttcat ttatacatat ttgtacaaaa taatttaaat aagttagtgg catacttgat
    10321 gttgaaggat ctatcagtgc tgatatagat gtccaaatca tatctctctt tagtaaaact
    10381 gatagttcta tactgtaacc tgttataatt tctataaata acttcgataa aaaaatcaaa
    10441 actataccta aaatagaaat cacaaagctt aaaataatta aatctatgac aatagctttt
    10501 atacaacttt ttcgatctgc tcttatactt aaagctcctg aaaagtcttt atttgctata
    10561 tttactccat acacaaatag cagtatagtt atatatgtta aaataggatt cacaatcgtg
    10621 gataaattag ttttagatgc ttctccaaaa atcttgagga taaacactgg caaaatattc
    10681 ataacaattc ccacaatcat aaatataatt atatacaatt tagtattttt attaaaaaat
    10741 tttatatatg gctttaattc attcaattac aattcctcct tcttagtcat gttaacaaac
    10801 atatcttgta gccctatgta tcctacatca atatttgaat ttttaagtat tttttcatca
    10861 ttttcactaa aatcaccata gtaaaaataa gcaactgcat ttccaaatgc ctttttaggt
    10921 tttatatttt ttatacattc taatttttct aactcttctt tatttccagt aatataatga
    10981 gctttttgtt ttatgacatc aatttcttca tctataataa tttttccctc atttaatatt
    11041 attacatgct ccagtaaatc atctatttca tcaatcaaat gagttgatat tattattgtc
    11101 cttggatttt ttatataact atctaaaatt atgttataaa actcttgtct atttactgca
    11161 tccaaaccaa ttgttggctc atcaaatatg gtgatagctg aattagaaca aatacctatt
    11221 atatttgaaa gtatagtttt catacctctt gacagttgtt tatacttttt tttagtattt
    11281 aaatcaaaat atttgcataa ttcttcttct aagcctttat cataactttt ataaaaacta
    11341 gaagaaaatt caaatatctg acctacttta aaatctggac tataaaattc tttttccctg
    11401 acgatacaaa cttcttccaa aacttttata tcttcttttg ggttctttcc taatatttct
    11461 atctcacctt cattttgaat taactgattt accattatat ttaataatgt tgtttttcca
    11521 gctccattct tacctaataa accatatatc ttattttttt caaagtttag agacatatta
    11581 ttaaaaatat tttgcttctt aaaactgtga gataaatttt ctatcttaat agaactcctc
    11641 atatattaat cctccttgaa attaattata atatcaacca attcttctct atttatttct
    11701 aatttgttag cttcttgaag taggtttttt acaaaattat gtttaaaatt ttcttttcta
    11761 gcttctttta taatagtttt tgcaccatct gatacaaaca tccctatccc cctcttctta
    11821 tataaaatat ttttatcaac caatatattt atacctttta atactgtagc tggattaatt
    11881 ttataaagct ttgaaagctc tgttgttgat ggaacctgtt cttcttcttt tattccattt
    11941 gatagtatct catcttcaat tgctcgtgct atctgaataa atataggttc ttcattattt
    12001 aatactaatt tcataggttc ctcctttctc cattagttag ttactcaact aattaactat
    12061 ataacttata atatcttgaa aaatattttc tgtcaatact aaataaaaaa cagagggaaa
    12121 attccctctg ttttttgttc tttatattta taattaaatt ttgatttttg taatcctatc
    12181 ccataagtta aaaactttaa taatctgttg tatcttcatt ttttaatatt ttacatattg
    12241 agctagttcc taacctttct gctcctgcct ctataaattt ttctgcatca gaaattgatt
    12301 taactccccc tgcagctttt atttttacat tttttccaac atgctctttc ataagcttta
    12361 tgtcttctaa agtagctcca cctgttccca ttccagtaga tgtttttata aaatctgaac
    12421 ctgacattgt tactatttca cacattttta ttttttcatc ttcatctaaa taacatgtct
    12481 ctataatgac ttttaatact ttatctccta tagctttttt tatttctttt atttcttttc
    12541 ctatattatc ataatcttta ttttttatat ctgaaatgtt tacaaccata tcaacttcat
    12601 ctgctccatt ttcaatagca tcttttgctt caaatacttt tgtagcagta gtttgatatc
    12661 ccaaagggaa ccctatcact gtacatatct ttattttacc ttttagatat tctgatgctc
    12721 ttttcacata tgatggtggt atgcaaactg atgctacact catatctaca gcttcatcac
    12781 ataaaatttt aatatcttcc catgtagttg tagcttttaa aatagtatgg tctactgttt
    12841 ttaatatatg tttcataaaa tcctcctaaa tttataatta atataattag attacttttt
    12901 taaatgcaat tttctctttg caatattcag tatcatatca acctcttcaa ctttaaaaat
    12961 agtcattagt agtgtataaa ttccacctcc aactataact gaaatagcta gtgatacaaa
    13021 ctcattaaat gttcctactc ccaaaattcc aaatataaat ttatacgtaa agtatgataa
    13081 aacacccata attaaagatg caactaccga tttcaaagtt gcttttatta ttttatcttg
    13141 tccaaaatat ccaacttttc tctttaggtt caagaagagt aacaatatac atgcaattga
    13201 tgatgaacta gttgcaagag caagaccacc atgagccatt ggttttatta aaactaagtc
    13261 caatactata tttaccccta cagatataat cccatttacc ataggtgttt ttgtgtcttg
    13321 taatgaataa aaaacctttc ccaaaatatc tcttaatcca aatgcagtca taccaactgc
    13381 ataaaaaatt aaggcagttg ctgtcatttg ggttgccctc gcatcaaaag cacctctttc
    13441 aaaaatgatt ctaacaacag gagttgcaaa aaaaatagaa gcaactgaaa taggaatcat
    13501 agaaattatt atcatattta tactagattt aactgaacta gtaaacttct tttgattatt
    13561 ttctgcactt aacttagata acataggata cataacagat actattgaag ctgtaaatgt
    13621 ccctgtaaca aaaccattta atctatctga ataagtaagt gctggaatgc ttcctttaac
    13681 taatgttgag gctaaagttg tatctaccaa tgagttcact tgattaacag caacacctat
    13741 aaatactggt gaaagtaatg ctagtaattt aataaggcta tcatctttaa agtttaagta
    13801 gtataagtat ttgtagttcg tttttttaac aaaaaacatg taaaagagca gttgaactac
    13861 catagcaacc acagcaccaa ttggaagtat gtatggtcca aatacggtac ttaacattat
    13921 tgaaataatt attactatat tatagggtat actcccaaat ccaactacaa taaagttctc
    13981 tttaatttgc agaaatgctg acataacact cgtaatacct atgaatatta ttcctgttat
    14041 caatatttta gtaaatttta cggtaagtaa aaacctctct ccttcaaatc ctatagcaaa
    14101 tatacttaca agttgctttg agaaaataac tcctaatata gctacaacta tgcatatacc
    14161 aactattata tttaaaacat tgtttaaaaa ctttagtgct tgcttttcac cttgtttact
    14221 acttatatct tgatacattg gaataaaagt agtaactata gcagtaccta ttgctgcaaa
    14281 tataatattt ggtatattca tagctgttaa ataactctct gtatataagc ctgtaccata
    14341 aattgatgat aatactaatt ctctacccat tccaagaatc ttagagatta tagtaactat
    14401 catcaaataa aaagttgctt tagctacctt gctcatttta tcactccact tctaacatac
    14461 ctctttttaa tcaagtaaaa tttggctcat tagatataat tctatcaact aatttgcctt
    14521 gcttagccct atcaaaatat aattaacacc tatttcttaa tatatcacaa actacattat
    14581 tcttcatatt tttttatttc tccaataagt tttacatctt tcctatataa attgtatagc
    14641 ttcaatt
//
LOCUS       DAEPOG010000100        14396 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEPOG010000100 DAEPOG010000000
VERSION     DAEPOG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.100"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            126..668
                     /locus_tag="KR432_002412"
                     /pseudo
     CDS             126..668
                     /locus_tag="KR432_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            813..1475
                     /locus_tag="KR432_002413"
     CDS             813..1475
                     /locus_tag="KR432_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0640941.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1475..2656
                     /locus_tag="KR432_002414"
     CDS             1475..2656
                     /locus_tag="KR432_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0640942.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(2837..5497)
                     /locus_tag="KR432_002415"
     CDS             complement(2837..5497)
                     /locus_tag="KR432_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640943.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5494..6177)
                     /locus_tag="KR432_002416"
     CDS             complement(5494..6177)
                     /locus_tag="KR432_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640944.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(6521..7459)
                     /gene="blaCDD"
                     /locus_tag="KR432_002417"
     CDS             complement(6521..7459)
                     /gene="blaCDD"
                     /locus_tag="KR432_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0640945.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(7487..8203)
                     /locus_tag="KR432_002418"
     CDS             complement(7487..8203)
                     /locus_tag="KR432_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640946.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            8535..10778
                     /locus_tag="KR432_002419"
     CDS             8535..10778
                     /locus_tag="KR432_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0640947.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            complement(11040..13154)
                     /locus_tag="KR432_002420"
     CDS             complement(11040..13154)
                     /locus_tag="KR432_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0640948.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(13412..14272)
                     /locus_tag="KR432_002421"
     CDS             complement(13412..14272)
                     /locus_tag="KR432_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640949.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
BASE COUNT         4944 a         2247 c         1877 g         5328 t
ORIGIN      
        1 ataaataact tatgtaagaa ccgtaggaac tacggggatg gcctgtggag aattagtaag
       61 acatatttta gtatgcaaaa ttctatgaag caggaaccct gtgactttag tcatgggagg
      121 ttcagaatac aactatgaat gagttggcaa aagaagttga atatactaaa cgcaccatat
      181 acaagtactt ttcatgtaaa gaagatttat tctttgctgt tgtcttaaga ggatataaaa
      241 gactttggga taatgttaaa atagaaagtg caaaaggcaa aacagggttt gaaaaaataa
      301 aactttcata ctttgctttt cataaatttt actctgtaga accttctcta ttatgcctta
      361 tgggaatgat tggtattgta aactctgaaa aatcggatac tgatatgctg tttaaagaaa
      421 agtttttttc ttttaataaa tttatgtttg atgaaataca aggaatgttt gaaattggaa
      481 aaaatgatag aagtattagg catgatattg aaatacctac tctcatgtat tcatcaatat
      541 ttacacttac aggatttttt aatttactat ctgtaacagg aaagtcatac ctgaacaact
      601 ttaatataga tgaggaaaaa tttattgaaa caactcttgc tcttttaata gattcaatta
      661 aagcataaac ctaagtttat aaattaaagt ttatatactt aaatgataat tttttctatc
      721 tctaacttct atctctaaca taaaaaatat taaacataaa actaataact tagttaatga
      781 aataactaaa ttactgaaag gactaaaagt aaatggctaa aatacttctt ttagaagatg
      841 atgatacaat tacatttgga ataaaaactt cccttgaaaa gaaaaatcac actgtatgtt
      901 gttataatac gattgctgaa gctaaaaaaa attttgataa aactattgat ttaattctgc
      961 ttgatttaaa tctacctgat ggtattggat acgagttttg ccaatatgtt aaaagtatag
     1021 aagatactcc agtgatattt ttgacagtcc gtgatgatga aaaagacatt atacgtggat
     1081 tagatatggg tgcagatgac tatgtggtta aaccatttct gctctcagta cttcaatcca
     1141 gaattaatgc agttcttaga agaactcatt caaatactaa caatactatt acatgtggaa
     1201 atataagctt gaataaagtt gaagtcaaag catatttaga taatgttgaa gtacttttga
     1261 cagcaggaga atataaactt cttctaagtc ttatggaaaa taaaaataga gctatgaccc
     1321 gtacaaagtt actagaaatg ctctgggatg tagatggtaa tttcgtaaat gataacactc
     1381 ttactgttac aataaagcgt cttagagaaa aattaaaaaa tccaccttgt ataaaaacat
     1441 taagaggcat aggatataga atggaggaca gctagtgaaa actccaagaa atatcaacag
     1501 tacagttgtt tttataatat tctgcttaat tataactttt ttattaagtg taattctaac
     1561 aactataact tcttttaatg aatacaatgt actgtctaag gctcttggtg catcatatga
     1621 gtacaatcct aagtctacag aaagtatact aaaatcactg aaaaattcta gtgataagga
     1681 ttttaaaaca ggaaatgaaa ttttatcaaa acatggatat tctaaaagta cattttggag
     1741 taaaaacttt ttttattttc tagtgatatc gtttggtata atttcaacaa tggctacagt
     1801 tgtatatata atcaaacata gaacaaataa gcaaaaactg agtagaataa acaatcttac
     1861 tcaatactta gaagaagtga atttaggaaa agagtctgta cttttacgat gtgaagatga
     1921 attttctcac cttgaagatg agatatataa aacagttggg gaacttcgtt gttcaaaaga
     1981 atttgcttta aaagaaagac aaactctttc agataatctt gcagatattg ctcatcaact
     2041 taagactcca attacttcta tgtcacttat ggcacaatta ctttcagaaa attgtccaga
     2101 tgaagaaata gattatatta acagacttac aaatcaaatc tcaagacttg agagactagt
     2161 ttcatcttta cttacattat caaaattgga tgcaagtact ttaatatttg aaagcaaact
     2221 tatagatgta cattctcttc ttacttatgc aattgagcct atagaaggtt ctttaagaga
     2281 aaaacatcag acatttacta tcgaggcttc tccttcggta gaatttgaaa tcgatattaa
     2341 ttggacttta gaagctcttt taaatattct taaaaattgt agtgagcata tagaagatgg
     2401 tggatatata gttgcttatt atagtcaaaa tcctctatat gttgagatta tcatagaaga
     2461 taatggtaaa gggtttgctc aggaagaact tccatatatt tttaatagat tttatcgtgg
     2521 taaaaatgct agtaaggaca gtattggtat agggcttgct ctatcaaagt ctattataga
     2581 acgtcaaaat ggtactctac atgcagaaaa ttgccataat ggtggtgcaa gattcataat
     2641 taaattttac acataaaatg ctggattata ggtaaaatac ctataatcca gctatatcta
     2701 tattctatgt tatctattgt tttagtaata tacttctatt tacatttaag gttttattaa
     2761 acaaacaaat taactatccc tatacttgta agtagtctta aatcgaaacc tttaaataag
     2821 ttcaaatagc ttttgtttat attgtttcat tttttataga ttctactata ttttcattct
     2881 tcaactgctt gtatcctgtc atatatgatg ctattacaat aaaaagtatc aatatagtaa
     2941 aacctattat tgtagatata ggtgcaaatg gtaaatactc tataaaatat atttcattta
     3001 tacgtagaaa agcatacact attcctattt gcacaggaat actaagtagt aatggtgtaa
     3061 ttcctaagaa caaaccttcc aaaacaagca tcttttttat gccttctgga gataatccaa
     3121 cagaacgcaa catagcaaac tcttgtcgtc tacttctagt atttcctgac actgtagcaa
     3181 gtacatttga taatccaatc atagccaata aacctgtaat aaagcctact attaaattca
     3241 tggtctttat tccactagcc ttttgttttt cactttctaa aacatcctca atactataat
     3301 ctcctgaacc atagtcttta ttataaatgg actctaattt ttctcttact ctaggaatgt
     3361 cctcacgatt atttacttta aatactccta tcagcctatt tgaacgtaac ctacttactt
     3421 catcataatt tttagatatt tcctgcacca tagacattgg cactatttgc attaatgtat
     3481 attttgaact agtatctact gttttaggaa gtgtgcttgt aatagctccc actttcatgt
     3541 tgtaagtata atccccttta tcttcgtcat atactttttc atttaattta atgttatctc
     3601 ctattgaaag atttaaataa tctatatata ctttatttct acgtgtacta cgattaatat
     3661 cttcctcctt attatataca attgaggttg gattttttgt atcataaaat aactttgggt
     3721 ctacagaaat ttgcctacaa tattctgtaa aactcttatc atcaagccca attatatttg
     3781 aaattatacg aaacttgcca tcacgttcta taggagagta ttttttagat gaaactatac
     3841 ttttaaatcc tccatttttc gcaagttctt ttgatgcatc tttttcagtc agccaagtgg
     3901 aagcagacat tttttgataa tacagtgaac tttttacacc ttctgtatta tctaattttt
     3961 caatgaaagc agactctaca ggttcactat tttccaaata aactgctata tctctttggt
     4021 ccttataatt atcacttcca taaacagctt ttgctccttc ttgatttgca attatatgca
     4081 aaaaacatgt aagtaataaa aaagataatg ttaaagatat tgttgcagta cgataagatt
     4141 ttttacgagc acgtagtgcg tttgatgcaa gttctccttc tattccaaat atctttccta
     4201 atgaatattt atgcgacttt ttgattttaa cactatctcc ttgtcgtatt gcttctatag
     4261 gagatatctt tgatactttt cgtgctggta taagtgcaga aatccagact gttactattg
     4321 taagtaaaac tgctggcaga aaagcaggaa taccataagt aaataccact tctggaacat
     4381 cagtatgata attaactgaa ttaatataaa ctataagcct attacacaat aaccatccta
     4441 aaaataaacc tattggcaat ggtataattg taagcaaaaa accttcaaac actacagaca
     4501 ttttaatctg ttttggtgat gcaccaacac tagataatat ccctaattga gtaagtctag
     4561 catttgcaga tagtgaaaac gcgttgtaaa tcaccattac aaacacagct actgtaaaaa
     4621 ttgctattgt aagatacata ataggagttg aaaattttga tatactgctc atagatgctt
     4681 tctgttctgg ggaaaagact aacattttta aaagataatt actattatat ttaagattgt
     4741 attttccata ttcattagtc ttccacccca atgatttagc aagttttgga agttccttgt
     4801 atgtgtctct tatattttta aatcgcaagt acacagttat actatcatta ggttttatac
     4861 ttgttctatc aagatatcca agagctgtat atgcaggtat tactgatgaa gtggaaacat
     4921 ctacctttcc tactattgtc aatgtagtct ctgaaatttt tttaaaactt tcccctttcg
     4981 atttttgagc tatgggttca agaggctttc catttaggat tctatctcct attggaagag
     5041 taattttatc cccaattttt acacttggat tattctcaaa atattgtttt gataatgcaa
     5101 tttcattagc attatttggt cgacgaccct ctattatagt atccttttct ggcatagaat
     5161 tccaatactc tgatgtagca tttctataaa ttatataatc tcttttctca tcattaattt
     5221 tgcctacata ccattgtcct tttaccatta catcatcaac tgaagaaaaa ttttcaatta
     5281 atggtaagtc acttccataa gtatagtcaa acaattcacc atgccagtta ccttctttct
     5341 caattgaaag tcttataaaa tctgtccaca tggtatacaa cagtccacac aaagaagaca
     5401 tcatggttgt agtaagtaat atagctaaca ttatagctat actactacgt ttattgcgtc
     5461 gtacaaagtc taaggtgtat tcttgaagaa tattcatttc ttcaccacct tttcagagac
     5521 aatcactcca tcttccatag ttacaatacg atttgcctct agtgctattt tctcatcatg
     5581 agtaataaga agtattgtct gattaaatcg tttatttgat aagtttaaca agtctacaat
     5641 ttcttctgaa ttttttctat caagattacc agttggctca tctgctaata aaatagctgg
     5701 tctatagatt aaacttctag ctatagatac cctttgttgt tgtccacctg aaagttgact
     5761 tggaagatgg tttaatctat cagataatcc caatatagat acaatctcag agaatctatc
     5821 ctcatccacc tttcttttat caagtaacat gggtaataat atattttttc taacatcaag
     5881 ggtaggtata agattataaa attgataaat aagacctact tttctacgtc taaaaactgc
     5941 aagagcttct tcctttaaac ttgatatttc tgtatcttca acataaatct ttccagatgt
     6001 aggtctatct actcccccaa gcagatgtaa caatgtagat ttgccagaac cagatgctcc
     6061 tacaatggat acaaaatcac ctttttgaac tgataaattt acattattaa gggctgtcac
     6121 tctggtttga tttgaaccat atatttttgt gagattttca cattttaata tttccatata
     6181 caatcatctc ctttatggtt aaattttagc aacctaaaat gacattatag tgacatgaat
     6241 acaaatataa attactatta aaaatattgt ttagtttttg cacattattt aaaattataa
     6301 tataaaacaa aaactatgca atatatttta tttatacttt taatatatta catagtcttt
     6361 aaaaggtatt aactttaaat ctataggtat tcaatttaat aatcaatagc aagtatttta
     6421 tgtactaaag gagttttgct ctatatagac tcaatttagt aaaattattt aataaatatt
     6481 acagactata ggtaaataat atttacaaat aaaattaagt ttattccctt acactataat
     6541 atttcttaat tatattaatt gcaatttcct ttacctttgg ccctgtaatt tctttattgg
     6601 aatcatctga tttaatagca aaataatagg tgtcaccttc acgctctagc ataccaacaa
     6661 accatgcatc cacacgttta tttttttcat caaatcctgt tccagttttt ccatatacat
     6721 ttatgttttt atcatttaca tctattttca taatatctct taaaatatta atatgctctt
     6781 ttttaaagtt tgtatctccc tcaaatattt ttgccattgt ttgcacttgt tcttttggag
     6841 atatttgtaa tgaagattct aaccaaaatc catttaaatg tccctttcca ttttttaaat
     6901 ctccttccca ttcgctaata tcgcaattcc catactttaa atcatctaaa atgttttgga
     6961 cagatttagc atcaacttta tctatcaatt ttttgtagta ccaaacacaa gactccttaa
     7021 atgcctcttc taaactaaga tttttattcc aatttttatt tggatactct gtaccatcat
     7081 atcccataac tgattctttt gagtttatta cccctttttc taaaccaatt aaagttgaaa
     7141 ctattttaaa agtagaacat ggtgaccttc ttgtttcaat taattctttg ttatatatat
     7201 tatattcttt atttttagta ttataaaaaa tagctcctcc actaatacct tcaaaacagt
     7261 cactatagtc aaccatatta attttttctt ttttgttttt atcattgttt tcaactttgg
     7321 atttatttaa ttcagagttt atatgattct tatcatgttt ttgttttgtg ttgttatgct
     7381 ttcttcctac ataatatact cccatagcta caacaactag aagaataact atccaaataa
     7441 aattattttt tcttttcata ctttatacca tctcctctac ataagtttat agttcacctt
     7501 taaaataata tacattattt gtaatatact ccaatctagg agcttgaata cttctattta
     7561 taaaatctaa ctttggttta acatgtttta aatctttaaa aatcacaata ccatcaatag
     7621 attgtaattc agctccaaag aaaaaataga ctaaatttct attcccagaa attttaacta
     7681 catctaaatt tctgtttagt tttttcattt tttttattac ttctttagaa aatatcttct
     7741 ccaattcatt tttatcagat tgaatataaa agacagattc agagcttaac tttttttcat
     7801 cataaacttt caaaatagct ttagctacta cactataatc tttttcataa agtttaaatc
     7861 taaaactttc acttggacta ggaataaata tagtacctat acttataaag atactaaata
     7921 tacatccaat catttttctt ttatttaaat cttttttata gtaaagctta atcatatgta
     7981 cacaaatcca tataaatgca attagcatca atggtattgc aaataaacct ataaatagta
     8041 gcattactcc tcccctacat attatatttc cacacaaaaa taaaaaatca cttacaataa
     8101 atagagttaa agggataact atatctctta tattttcttt acctaatata attatgttga
     8161 ttgaggctat gatgaatata ctcaatataa ctattagtat cattatgtcc tcctttcagt
     8221 ttgtgaggta attatttatt ctctatttaa aacttactat tgtaatattt aaatattaca
     8281 atagtaagtt ttaaatgtca agtcgtttta attgtataat tatctttgta cttatcaaat
     8341 tacgagtatc tattatatac ttttttatct attatcaact atcatttatt cagattgtat
     8401 gggattggtt aaatttagaa ataccttcta taacatagca gaatatatca agaaagtaga
     8461 ttttaatttt attataatat ggttacataa tataattgct ttactaaaat gtactcagaa
     8521 aggatttaac attcatgtca aatattataa taaaaggact tacacaaaat aacctaaaaa
     8581 atatatcatt taaaattcct aaaaataaaa ttgttgtttt tactggagtt tctggttctg
     8641 gaaaatccag tatcgtattt gatacaattg cagcagaatc acaaaggcag atgaatgaaa
     8701 catattcttc atttatcaga aataggctac ctaaatatag aaaacctcag gtggagctga
     8761 ttgataattt aactgcttct gtaatcgtag accaaactcc tcttggtggc aatgcacgtt
     8821 ctacagttgg cactataagt gatttgtatt catcattaag attattattt tctagaattg
     8881 gagaacccta cataggaaca acttcatatt tttcttttaa tgacccaaat ggtatgtgta
     8941 aaaattgttc tgggattggt agagttaaag taatcaatat tgaatctatt ataaatcctg
     9001 aaaattcatg gaatacagga tgtataaatg actctttatt taaacctaat tcatggtact
     9061 ggaagcagta tgcagagtct ggactttttg atttagataa acctataaaa gattattctt
     9121 ttgaagaata taatttattg ctttatggtt caaaaaataa agatggaaaa caagaaaatc
     9181 caaaaataga gggtattttt aacaaataca atcaaacata tctaaatcga gatattagca
     9241 atatgagcaa acacacaaaa gaaaaatcag aaagacttat aacagataag gaatgtcctg
     9301 tatgtcatgg aaaaagatta aataaagaaa ctcttaactg taatatttta ggatattcga
     9361 ttgcagatat gtgcaatatg gaacttactg acctttatga tttattgtta caaataaaag
     9421 ataaaagaac tgaaactgta ataaagactc ttactgatgg tttaaaaaga atgattgata
     9481 ttggtctccc ttacctacat ctaaatagag aatcatcatc tctatctgga ggagaagctc
     9541 aaagattaaa acttgtacgc tatatgggga gcagtctcac aggtatgact tatattttcg
     9601 atgaacctag tgctggtatg catccacgag atgtacatcg tatgaatcag ctattaaaaa
     9661 aacttagaga tagaggaaat actgtccttg tagtagagca tgataaagat gttatctcca
     9721 tagcagatga agttatagat attggcccaa gtgctggtac tcagggtgga catgttgtat
     9781 tccaaggctc ttatgataaa ttacttttag caaacactct tacttcaaat gcaatgaatc
     9841 tatcattacc tataaaaact gactctcgtc atccaaaagg tacccttcca attagagatg
     9901 cttgcataaa taatctaaaa catattgatg tagatttacc agttggaatt atgactgtcg
     9961 ttactggagt agcaggttct ggcaagagta ctttaatctc tcgtgttttt gcacaaaaat
    10021 acgaaaaaga tgttattaaa atcgaccaaa gcccagttac agctacaaat cgttctatgc
    10081 ctgctacatt tcttggtttc tttgatgaga taagaaaagt atttgctaaa gaaaataatg
    10141 ttgaagaagg tttattcagc tttaattcaa agggagcttg tcctgtttgt gatggtaaag
    10201 gagtacttgt tacagagctt gtattcatgg accctattat taatacatgt gaagcttgca
    10261 aaggtactag atatagtgaa gaagttttgt caaaattata taaaggtaaa tgtatagttg
    10321 atgtactatc tatgacagta gatgaatctc ttgaattttt tacagaaaag aagctatgta
    10381 aaatcttaca aacaatgaaa gatgttggtt taccttatat gaccttaggt caaccactta
    10441 gcacactatc tggaggagaa cgtcaacgaa ttaagttagc taaaaacctc aacaaaaaag
    10501 gtactatata tattttagat gagccaacta cagggcttca tggttctgat attgaaaagc
    10561 ttatgttgat ttttgagcat atagttgata aaggtaatac tgtaattata attgaacata
    10621 atttggatgt attgaaacaa gcagactggg taattgatat tggtcctgat ggtggcaaaa
    10681 acggtggtca ggtcgtattt gaaggtacac cactagaaat gtttgaaaga ggttgcacta
    10741 taacatcaga tttccttaga aaatcaataa atatatagat aatataatac taatattaaa
    10801 aaattaagaa ctattacata tatattacta ctttgtttta tttcataaat ttaaagttta
    10861 actttatttt aaaagaaatt aatattataa aagctataaa tcacaaaatt ttataaaatt
    10921 tcaaatgtaa tttatagctt tcatttttta taaatttata agtattattt tttgttgatt
    10981 tattgatgaa tctttgtccc aatttatatt tacgataata aatgcatata agatataatc
    11041 tacatgctta taagttgagt taatgtacca acatatgtta acttaagttt gtgcatagct
    11101 cgtgtacaag caatatataa caaactgcgg tcataatcac tataatatgt tttattattt
    11161 actgaaggta caataacttc atcaaactct aaaccttttg acattttaat agaagttatg
    11221 gatactcctt ttgtaaagct agaactttca gaagatatta agtgaacact atattcttct
    11281 cttaacgcat catatacagc tttagcgtca ttatctgtct ttaatataat accaagagtc
    11341 gcattatcac tctttttaaa ttcttcaatt tctattttta tcttatttat ttcatcttgc
    11401 tcgttattac attttaataa agccggttct tcaccatgtc gttcaatagg ctctaatgaa
    11461 gatatatctt gaatcttttt ggcgaaatta ataatctcaa aagtagaacg gtaactcttg
    11521 ttcaacataa ctagttcccc atcattatat agctgtttca tatcatctaa tgtatgagta
    11581 tgattaggat taaccaattg tccaaaatct ccaagaatag tctttttaca cttaaaaagt
    11641 aagtttatta cagcatattg aataggtgta tagtcctgca tttcatcaat aacaatatgc
    11701 cttataatct tatcttcttg tataccttca tatgcagcat gaatatatat aaatgggtat
    11761 acatctgacc attcaagtgt tttcttttct gccatcacaa acatgtttga tatattcatt
    11821 tgtttaaaga aatccttata tagtgtaaga gtatttttta ttgtcaacat tccattcaaa
    11881 cttttaagta tactttttac ctttggtaaa tcttcttcca taatattatc tgattcaaat
    11941 ttataatgaa tgtcttcagc taccttttct aatctcttct taactggata tctgttatat
    12001 gcctcaaatc tactctgtat ccagtcactt ttagctgtaa aacttccaaa agtgtagtct
    12061 tttggaataa aaatcttatt tggcatttgt tttatataat catcaataag ttttacaaaa
    12121 tcaagtgtag atttaaaacg cactctttca gaccatttag catcatttat ttctaatggg
    12181 tctttctcac tttcaaaatt aataactcta tctaattgta cctctgctat attttcaaaa
    12241 cttaattcac ataatggttc ttctccaagc tcaggaagga cattggaaat atagtctcca
    12301 aagactttat taggtgatag gataattaca ttatttgctg atattttatc tttaaatcga
    12361 taaagaagaa aagcaattct gtgtaatgct atagatgttt tcccagaacc agctacccct
    12421 tgaataatca gtgtatcagc tttatcattt ctaataattt gattttgttc cttttgtatt
    12481 gttgaaatga ttgacttcat tttttcatct gaagtgtgac taagttcttt ttgaagaata
    12541 tcatcttgta tattaatgga actttctagt gcatattcca attttccatt tttaatttta
    12601 aattgtcgtt ttcttgttat ttctccatcc acccatccta ttggagcatc atatccagct
    12661 tttccgattt cataatcata aaacatactg gcaattggag aacgccagtc aagtattata
    12721 agctcatctt cataatcaaa tgcaaatcta ccaatataat acttagattc atcatccata
    12781 tctttcagcc taaaatctat tctagcaaag tatggagaat ctttgatttt attaatctta
    12841 tctcgaactt ttaccataaa agccccatag ttatctattt gttttagtcc ttgttcattt
    12901 tgaaacattt catgtgggtc tatttcacca cgatttttta ccatatacat tttagcgtcc
    12961 atataatcct tatcaagctt gtcaacaaga ttttcagctt tcttaaaagc attgtctagc
    13021 ttgttattta tctctattag atgtgcaatc tcatctggaa aaaaagttaa taactctaca
    13081 tcatgttcac acatattatg tttagaattg gatgcaataa tatcgcaatt tactacttgt
    13141 ttattttcag acatttaaat cctcctttgt taattaagta cttacactga atcattatac
    13201 ttaattgtgt tttggagttt ttaatcattc ttgctattta acttaaatgt tagcttttta
    13261 gctttaaagt tcttttttta aaaaactaaa tctttctata tttattacca taagtacact
    13321 tacaagcaaa atacattaaa agcagaaata ctcttatata taaaataatt caaacaaaaa
    13381 atatgtggta aactttgcat ttaatctaca attaattttg tctattatct ctttcttcta
    13441 cacaaggaag aataaatttt tgagtaggat atccaaattc cacaagcaac tctgcttctg
    13501 caaaccccaa cttctttatc atatctctat gtcctgtatc agctttgtca ccctctcgaa
    13561 aggtagtaat tgagatatct gtatctatca agaaatcttc aataactttc ttcaaaaaca
    13621 cttgtgcaat gtctgaatta cgatactgtg gatgtattcc aaaaaaatca atacttctag
    13681 tttctctatt aaacaccata tttgcaatag cgacattatt aagttttaat attaaagctc
    13741 tttcttctaa aatatactgt tctaattgat atatatattc ttcctcttgc aaatttggaa
    13801 atccatctat gactaatctc actaaattca tccataatgg tatgtcagac atacttgcaa
    13861 gtttaatttc tttttccaac tctagtacat tctttgaaaa taggttatct tttttcaaaa
    13921 caaatcttag ctgcaaagga taaaattctt gttctttcct atatttattt ggagattttt
    13981 tatacatagc tttaaatata gaagtgaatg cctgttgact ttcatatcca gctatcaatg
    14041 caatttcaat aattggtttc tttgaaaata ccaataattt tgctgcttct gtcaactttc
    14101 tccgctttat gtaatcatgc attgttagac caacagaagt tgtgaaagtt ctatgcaggt
    14161 gatattttga gtaatgaaca gcattagcaa ttatatctaa atccagcttt tctgataaat
    14221 gctcctctat atagtcaata gcagttatga cattaattac tttgtaagac attgcagctt
    14281 ccctcctttt atccatgcaa cattatatca attattactt agttatattt aatgaatctt
    14341 gctattttat aaacaaattt gttgtttaag ttttcaattc ataaatttaa tatatt
//
LOCUS       DAEPOG010000101        14142 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEPOG010000101 DAEPOG010000000
VERSION     DAEPOG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.101"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            1081..2454
                     /locus_tag="KR432_002422"
     CDS             1081..2454
                     /locus_tag="KR432_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0640950.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            2710..3777
                     /locus_tag="KR432_002423"
     CDS             2710..3777
                     /locus_tag="KR432_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBH0640951.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3874..4182
                     /locus_tag="KR432_002424"
     CDS             3874..4182
                     /locus_tag="KR432_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBH0640952.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            4175..5605
                     /locus_tag="KR432_002425"
     CDS             4175..5605
                     /locus_tag="KR432_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBH0640953.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            5683..6057
                     /locus_tag="KR432_002426"
     CDS             5683..6057
                     /locus_tag="KR432_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBH0640954.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            6051..8255
                     /locus_tag="KR432_002427"
     CDS             6051..8255
                     /locus_tag="KR432_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0640955.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            8255..9664
                     /locus_tag="KR432_002428"
     CDS             8255..9664
                     /locus_tag="KR432_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBH0640956.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            9649..10713
                     /locus_tag="KR432_002429"
     CDS             9649..10713
                     /locus_tag="KR432_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBH0640957.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10743..12164
                     /gene="nhaC"
                     /locus_tag="KR432_002430"
     CDS             10743..12164
                     /gene="nhaC"
                     /locus_tag="KR432_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0640958.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            12593..13057
                     /locus_tag="KR432_002431"
     CDS             12593..13057
                     /locus_tag="KR432_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0640959.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            13355..13717
                     /locus_tag="KR432_002432"
     CDS             13355..13717
                     /locus_tag="KR432_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0640960.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
BASE COUNT         5610 a         1526 c         2634 g         4372 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatata atttataatg tatacaattt attataaaaa atttaatttt
      541 aattttctat ttttgatact ataatatata atcttgataa tttaattaaa aatctagatt
      601 ttacatacca aactggtttc ttatttcgct atctttactt atgaactcat atattcctat
      661 aaaggttatt ttagaaaaag ttggttattt aaacattaat accacaattt ctatagattg
      721 tttgttgaat aatctataat tatattatgg actaaaaaat ccaatgagtt aatttattgt
      781 ttttttatat ttgagagatt acaacttatt agtttgtata tctctttttt taataaaatc
      841 aataatatat aaaactattg gcatatttaa tgacaattca aatattgtat ataaccataa
      901 tgaaagatat ataaataaaa ttttaaatat acttaaaaat atattaatat aatgtacata
      961 aaaaatatca taaatatatg ataagataaa tacaatagca atattttatt aattttttgg
     1021 gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt ttttaaaata
     1081 atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa agacgggaaa
     1141 actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca cgatatttta
     1201 gccaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac actattagaa
     1261 gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga caaaaatgga
     1321 aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga gtttgtaggg
     1381 gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaacag aaattataaa
     1441 aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt tgatgatatt
     1501 ttaggggaaa gtaatttgat taaagaagca atagaaaaat ctaagaaagc ttgcaagtct
     1561 gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt tagccagtcc
     1621 attcattatg gaagtaatag gagaaactat ccatttatag cacaaaattg tgcggctttg
     1681 ccggaatcac tattcgaagg tatccttttt ggaactagca agggaggatt tacaggagca
     1741 atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt ggatgagata
     1801 aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga aggttatgta
     1861 agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc aacgacaaat
     1921 gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata ttataggctt
     1981 aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat acttctattg
     2041 tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt aggaatttca
     2101 gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag agagctagaa
     2161 aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga aacaagtatg
     2221 attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa ttattcttta
     2281 aacgatttgg agggcaaaac acttagtaag attatcagtg agatagaaga aaagtatata
     2341 tatgaatcat tagaaaaggc atcgtacaat ataactaaag ctgcatcaat tttaggtatg
     2401 aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt ataaataagc
     2461 aagataaaaa aacaaaatag aagtaaaata aaaaagcaaa atataggagc aaaattatta
     2521 taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt attttttatt
     2581 tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat ttactttaat
     2641 tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa tttttaggag
     2701 ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat gggaataggt
     2761 atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg tagtagaagt
     2821 acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag accagaagtt
     2881 attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt tgttttgtta
     2941 gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt aaatagaaaa
     3001 ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga tgcagattta
     3061 gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg aacaggaata
     3121 aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg tgaagaagtt
     3181 actagtataa aagctaagag agtaaatgat ttatctccat ttggaaaatc tgttatggag
     3241 gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga taagactata
     3301 gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt aggttggaaa
     3361 ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag aaaatctaaa
     3421 tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata tggatatgta
     3481 aatggagacg ttttaattga aatggagcat ccacaacaga taataccaga agtagaaggt
     3541 attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt acaaataaat
     3601 cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat tccattaata
     3661 ataaatgcta gaccaggtct taaaactatg ttggatatac cagtgcctag agcaataatg
     3721 ggagacataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc taattagtaa
     3781 gataacagtt agctaattgg taaaataatg tttaatttaa aataacagta aattaattag
     3841 taaggaatag tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt
     3901 attatacaca atatagtatt gactccagaa gaaagagctc cacaagttcc agaggatact
     3961 aagaaagtat ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc
     4021 gacttagtag aagttaaaac aattacaggt agacttgtaa aaggtaattt acttaaagta
     4081 aatccatatt atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc
     4141 caagctaaag aaatattatt tggaggtgta tataatgaat aattcaacat caataaaaga
     4201 tatgagttat caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt
     4261 ggattattct tcttttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga
     4321 aacaggatat acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac
     4381 tcctctaata gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa
     4441 aggagctaaa atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag
     4501 aagagcagct actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc
     4561 taccagtggt aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg
     4621 tataatagtt caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa
     4681 agcacgaaaa tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt
     4741 attttatacg ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc
     4801 accatttgga attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga
     4861 aaagtataaa aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac
     4921 tggaactgca agaggattga taaaagctgg ttccattaat actaaagtag taggagcaag
     4981 tgtagattta aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac
     5041 aacaggacat acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc
     5101 taggtctgca gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga
     5161 agttttttat acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg
     5221 aaatacatct ttagcagcag cattctcttt agctcaagag ttagatgagg ataaagttat
     5281 agtggtacaa gaaacagaat atacaggcgc aggtaaacat atatgtccac agcttacttt
     5341 tgcaagagaa aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt
     5401 aaacttaata ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa
     5461 aataagaaag tcatttataa aaaattgcat atctaataat cacattgata atattgataa
     5521 gctaagtaat cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt
     5581 tgttgatgta ttaaataatt tatagtaatt attgtttttt tatttaatat aaaataaaaa
     5641 ttcggaaaat tctattaaat caatgcgaat ggaggaatgt tcatgaaaaa aagagaggat
     5701 gattttgaag ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg
     5761 ttttggaatt tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca
     5821 acaccatcaa tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa
     5881 cctattttag aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt
     5941 tataaattgg ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag
     6001 ggtgaatatt gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg
     6061 aaaataaaaa attggatata gactatatat taaaagattt ggataaatat aaaccaaaac
     6121 gaagaggttg ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata
     6181 aagactgtac aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata
     6241 atatagaccc tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg
     6301 atgatataag aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca
     6361 gaactgctgg acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg
     6421 gagttccaat aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag
     6481 aagaagttgg tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag
     6541 tagcagtaat gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg
     6601 ttctgtatag gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa
     6661 taatggcttt tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta
     6721 gagaagcatg gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatattct
     6781 cagaaaaaat aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac
     6841 ctccagcacc atgtttaaga atagacttac cttacgctgt tgcacttaga gaattattta
     6901 gtgattataa gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag
     6961 caacagttac tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat
     7021 caactataac acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat
     7081 gtgatacagc aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta
     7141 aagatgtcgg tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata
     7201 tggaagaaat atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg
     7261 attctggaaa ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag
     7321 ttggtgaagg tactgtttat gaaagagaag aagattattt agcacctgta actgctcact
     7381 ttggatacaa taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa
     7441 tagatggatg tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt
     7501 ttgataatgt agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta
     7561 agcctgaaat ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg
     7621 ataaaagaac agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa
     7681 ttgaggtcat aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag
     7741 gaagagttcc gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac
     7801 taactgatga agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta
     7861 cggttggaga agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag
     7921 gtattgaaaa atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat
     7981 tggtagatgc agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc
     8041 atgatgatat tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta
     8101 taagagataa agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg
     8161 aacaaggcat cgatgctgga tttggaagaa acagcaaagg catacatgta gccactttct
     8221 tagttgaaaa aagaagagaa atgagtaata aataatggaa aatagcaaag tttctttaaa
     8281 aatagatgtg ttggtagctg aaattggaag tactactaca gtggtaaacg ctttccacga
     8341 tattaatact aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga
     8401 aggtggcgat gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc
     8461 tgtagatgat atagagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa
     8521 aatgactgtt catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt
     8581 aggggctgga gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa
     8641 taaaataaaa gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga
     8701 aagagataca gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt
     8761 tatatatgct ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa
     8821 ttataagctt tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc
     8881 tacaagaaga atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa
     8941 atacattaaa gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc
     9001 ttctaaactt ttatataaaa atattggaga tttattaact ttggatgtag gtggagcaac
     9061 tactgatgta cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc
     9121 tgaacctact gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa
     9181 tatagtagaa gttataggta aagaaaactt acaaagtgaa ctatatatag atattgatgc
     9241 agtaatagag aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgagag
     9301 gttgacaaaa gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat
     9361 ctatggaaca actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata
     9421 tatagttggt acagggggag cacttactag gctccctagt agaattgaaa tattagataa
     9481 tatgctcaaa tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt
     9541 aatagataat gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc
     9601 atctcttaag ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg
     9661 ttagaaatag atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat
     9721 gaaagaagta ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta
     9781 gaagaaatat gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa
     9841 aaattacaag atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta
     9901 gaagaagtta tcaattattg cgatattagt tttaactcag aattagaaac aataaagaaa
     9961 ttaaatgaat tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta
    10021 ggcgatttac gagaaggata cttttatgaa gaagactttt ttaataatgt aagagagata
    10081 gtaagattaa aaaatattga aataattggt atagctacaa acttaacctg ttatggagct
    10141 attataccat ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa
    10201 aaatttggaa taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg
    10261 ataaataata atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt
    10321 ggtagagaaa cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta
    10381 atatgccaag tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt
    10441 gatgccttta gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata
    10501 gctataggta aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa
    10561 atattagggg caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat
    10621 aaattaggtg acaatttaga atttttatta acttatgggg gtatcatgtc atctagtaca
    10681 agcaaatatg tttcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt
    10741 ttatgaaaga gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt
    10801 gtttgatatt atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac
    10861 taataggagc agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga
    10921 aagagttaga attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca
    10981 taattataat aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga
    11041 tttactgggg actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct
    11101 gtgcaatagt atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag
    11161 ctcttttagg aattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa
    11221 tatctggagc ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc
    11281 cagcaatggc aggaacagat ttgtttacac atataaaata tatgttatat tcgactatac
    11341 catcattatt aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag
    11401 cactagatat aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt
    11461 tatcaccagt attattatta gctccagcaa tagtaattgg attagttgta tttaaagttc
    11521 ctgctatacc agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc
    11581 aaggagaatc tatgaatacg atattagatg cagcacaaaa tggatatgtt tcgtcaacac
    11641 aaatacagga ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg
    11701 tatccttaac aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg
    11761 aagtagtagc atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta
    11821 ctatggtaac ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta
    11881 taccaggaag aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt
    11941 cgagagcttt agaagatagc ggaactttaa cttctccatt agtaccatgg aatacatgcg
    12001 gggcgtatat aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat
    12061 taaatattat aaatccaata gtatctttag tattaattgc atgtaagttt aaaatagcaa
    12121 gaatagaaga tgaacctgaa acttgtttaa attttgatgt ttgataatag ataacttatg
    12181 taagaacgta ggaactaggg ggggagcctg tggggaatta gtaagacata ttttagtgtg
    12241 cgaaattcta taaatcagta atcttgtgac tttagccatg ggaggttcag attagtaaat
    12301 ttatagtttt atattagtct atactttgat cttttaattg tttgtataaa ttttttatag
    12361 aaaaagattt cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt
    12421 ataagagcac tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa
    12481 atggaaggag gttatgtttt atatttatat gaaataatta gttacacagt gaattttcgt
    12541 ataatttggt ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag
    12601 aatatccaat ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac
    12661 aattgacttt atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa
    12721 atatctggag gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat
    12781 ggatttagca aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa
    12841 aaatgagatt ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg
    12901 accttatgaa ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg
    12961 atataaactt catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag
    13021 tgaatatctt acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt
    13081 agttgataat tagaagaaca tttaatgcta ctgcataaat tttatcctaa ataatatcaa
    13141 ggtgatttaa tttatgtatt ctaatgtaac tgtagataat caatttataa ctaacttagt
    13201 gtaatttaga tttaatttat tattctaatg taaccccaat atattgttaa ggtctactta
    13261 agatgctcaa aaggctatgc ttaatttatt gttatataat ttgtagagtg gtataattta
    13321 gaagtgatac tatatagata tataggggga aaatatgaaa atatatgata tattaacacg
    13381 tttttatatt caagatatag aaaaagccat acctttttat gagaatcttt taaaagaaaa
    13441 atgttctttg cgtttttctt ataaagaagt tgggttggaa ttagcgcaaa taggaaatgt
    13501 tttactatta tctggttctg atgatgctct taaaccattt atagaaacaa aatctacttt
    13561 tatggttgat tctgttgatg aatggagaag ttatctattg gataatggag cagttgtagt
    13621 aagagataag aaaaaagttc ctacaggata taatatgaca ttgagacatc cagatggtac
    13681 aattatagaa tatgttcaac atacaaagca ggattagtta aaaatagatt ttactactga
    13741 gcaactaatt agaatctatt cttagacgta caatttatta tttttggtat taatagatta
    13801 taagtcaaat tataatattt caaaatagaa tagaaattta tttacataaa agagagtgtt
    13861 taaaaatgaa ctttttagtt cacgaagcac tcctttttgt atgaaatcaa atacatcaat
    13921 atataaaaca aactgatgaa gagcttaaat taaaataaaa agattcataa caggacccca
    13981 ttaaacttta agatagggta agtttgtaat catattaaat aattgtaaaa agctgttttt
    14041 agtcgtataa tataaatatt tatcatttaa ttaaggaatc taaaaggaat ctaataagaa
    14101 gaaaataata aataactttt tagatttact aaatgaaaaa tt
//
LOCUS       DAEPOG010000102        14125 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEPOG010000102 DAEPOG010000000
VERSION     DAEPOG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.102"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            530..1423
                     /locus_tag="KR432_002433"
     CDS             530..1423
                     /locus_tag="KR432_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBH0640961.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1423..2817
                     /gene="gltA"
                     /locus_tag="KR432_002434"
     CDS             1423..2817
                     /gene="gltA"
                     /locus_tag="KR432_002434"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBH0640962.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3183..5402
                     /locus_tag="KR432_002435"
     CDS             3183..5402
                     /locus_tag="KR432_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0640963.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5549..6295
                     /locus_tag="KR432_002436"
     CDS             5549..6295
                     /locus_tag="KR432_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640964.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6295..8175
                     /locus_tag="KR432_002437"
     CDS             6295..8175
                     /locus_tag="KR432_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0640965.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8294..9685
                     /locus_tag="KR432_002438"
     CDS             8294..9685
                     /locus_tag="KR432_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0640966.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9792..10832
                     /locus_tag="KR432_002439"
     CDS             9792..10832
                     /locus_tag="KR432_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0640967.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11321..11974
                     /locus_tag="KR432_002440"
     CDS             11321..11974
                     /locus_tag="KR432_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0640968.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            12050..12259
                     /locus_tag="KR432_002441"
     CDS             12050..12259
                     /locus_tag="KR432_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640969.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12399..12965)
                     /locus_tag="KR432_002442"
     CDS             complement(12399..12965)
                     /locus_tag="KR432_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBH0640970.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13126..13779
                     /locus_tag="KR432_002443"
     CDS             13126..13779
                     /locus_tag="KR432_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH0640971.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13937..14113)
                     /locus_tag="KR432_002444"
     CDS             complement(13937..14113)
                     /locus_tag="KR432_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640972.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5551 a         1387 c         2374 g         4813 t
ORIGIN      
        1 taaatcttta gaaattgtaa aaattttata taaaaattca aataattaat gaatattgaa
       61 aattttagat atataataaa ataagagtat attttatata atatgaaagc tggtattgca
      121 atagattaaa gccttataca cgtttagttt ttaaagaaat tagtgttatt atgaaaacct
      181 aagttaattt attaaataaa taattgaata aaaaaatttt attatgtatt tataaaattt
      241 ttgttacaaa tttggcataa tatagtatac tttaagtgtg tattaggtta aatctataac
      301 aaattagacg tatgatatag acttagtgtt tattggcatt tacaagaaat ttgttaatat
      361 caactatttg tagtttgggc agttataatt actatagggg ggatgtccaa tacaaaacaa
      421 tcaaccaaga actaatatta tattataaag agttatagtt ttgtgtgttt atgtttttaa
      481 ttaaatttaa agtgggaaaa atatatttgt tagaatggag aatagcgaga tgagtaataa
      541 aatagttagt aagagacaat taacagatag tatttattta atggaaatag aggcacctag
      601 agttgcaaaa tcttctcaac caggtcaatt tataataatt aaaaatgatg aaaagggaga
      661 gaggattcct cttacaatag ctgactatga tagagaaaaa ggaacagtaa ctattgtatt
      721 ccaaacagtt ggtgcttcaa ctaaaaagtt agcaatgttt gaagaaaacg attttgtaat
      781 ggattttgta gggccattag gacaagcaag tgaatttata catgaagata tagaagaatt
      841 aagaaataaa aagatattat ttgtagctgg tggagttggt tctgctccag tttatccaca
      901 agttaaatgg tttaaagagc atggattaga tgtagatgta ataattggtg caagaactaa
      961 agaattaatt atattagaag atgatatgaa aaaagtagct aaaaatgtat atgtatcaac
     1021 agatgatggt acatatggat ttaatggaag agttacagac ctattaaaag atttagttga
     1081 taatcaaggt aaaaaatatg accaagcaat agtaattgga ccaatgataa tgatgaaatt
     1141 tatgtgccaa ttaactaaag aactaaatat acctactata gtaagtttaa atacaataat
     1201 gatagatgga acaggtatgt gtggtggttg tagagttagt gttggaaatg aaactaagtt
     1261 tgcatgtgta gatggtcctg aatttgatgg tcatttagtt gattttgatc aagcaatgag
     1321 aagacagtct atgtacaaga ctcaagaggg aagagctatt ctaaaacttg aagaaggcga
     1381 tagtcatcat catagtaatt gtggatgtgg aggtaacaaa taatggatgc taaaaaagta
     1441 aaagtaccag ttagagaaca agaacctgct gtgagagcaa caaattttga tgaagtatgc
     1501 ttagggtata ataaagaaga agctatggca gaagctaata gatgtttagc ttgtaaaaaa
     1561 ccaaaatgtg tgggtggatg tccagtagga atcgacatac cagggtttat aacaaaaatt
     1621 aaagaagatg atatagaagg tgctgctaaa gtaatagcta agagcagttc attaccagct
     1681 gtttgtggta gagtatgccc acaagaaagt caatgtgaag gtgtatgtat acttggaata
     1741 aaatcagatg ctgtatcaat aggtaaatta gaaagatttg tagcagattg gtcaaaagaa
     1801 aatgacataa atttatctga tacagagcct aagaaaaatc aaaaagtagc agtaatagga
     1861 agtggtccag caggtctagc ttgtgctggt gatttagcta aaaaaggcta tgatgtaaca
     1921 atctttgaag caatgcatga gccaggtgga gttttaactt acggaatacc tgagtttaga
     1981 cttccaaaac aagctgttgt tcaacctgaa atagacaata taagaaaatt aggtgtaaaa
     2041 atagaaacta atgtaattgt tggtaaaaca attacagtag atgaacttat agaagatgaa
     2101 ggatttgaag ctatattcat aggttcagga gctggtcttc caatgtttat gaatatacca
     2161 ggggaaaatg caaatggagt gttttctgca aatgagtttt taactagagt aaacttaatg
     2221 aaggcttata gagatgacta tgatacacct attagttctg gtaaaaaagt tgctgtagta
     2281 ggtggtggaa atgttgctat ggacgctgct agaactgcat taagattagg ttcagaatca
     2341 tatatagttt atagaagaag tgaaaaagag cttccagcaa gagctgaaga agttcatcat
     2401 gcaaaagaag aaggcattat atttaataca ttaacaaatc ctaaggaaat tttagtagat
     2461 gaaaatggat atgttaaagg tatggtttgt ataagaatgg aattaggaga gccagatgat
     2521 tctggaagaa gaagacctat agaaatagaa ggttctgaat ttgtacttga tgtagacaca
     2581 gttataatgt cacttggaac aagcccaaat ccactaatat cttctacaac aaagagtctt
     2641 aatataaata agaagagatg cttaataaca gatgaaaatg gtcagacttc taaagagggt
     2701 gtatttgcag gtggagatgc tgttactgga gcagcaacag taatatctgc aatgggtgct
     2761 ggtaaaacag cagcagcatc aatagatgaa tatttaaaaa caaaagttaa tgcttaattt
     2821 gtaaattaaa gagagggtgt ctcaaaatga actttttagt tcatgaggca ctttttttgt
     2881 acaaaatcaa atatgtttta atcatttcaa caatgaaaat ttcattcctg tagaaagtct
     2941 aacttttaag tcttagtaca atctctattt tgatgtaggc acttttttag atggtttaat
     3001 aatttatcag ttattattgt aatatttgat actaagtaac ataactttgt ctaactatga
     3061 taaaatagtt atacaataaa ggtcttgtaa ggagtgaata atgtgtggct aataaaatca
     3121 gatttgtcaa atctaacatt aaataataga aaaataaatt gtgtaattaa tggtatttta
     3181 atatgaaaaa aagagaaaaa gtatttgatg ggctagtttt taagcatata ttaatcttat
     3241 taatactatt agttttgatt gatttaacaa gtaccatcat atatgcaagt aactctatgg
     3301 aaattacttc tagaaatatg attgaggcat caaaaagaga acttgaaaac tatttagaag
     3361 taaacatatc tttattaaaa gctcttagtc aagatgatag attttctaat ggtgaaacat
     3421 cactaattga aaagggaaaa ttattaaggc catatcaaaa ggaatataat ctttttatga
     3481 ttggtataac agatacaaaa ggaaatgctt caagtacata tagggaaaat gttaactcaa
     3541 taaaagataa gcctagtttt gagaaagcaa ttaaaacaaa gcaagtagta gtttcagata
     3601 ttgaagtcag taatgtaaca ggtgacaaag tatttatcat atatgtacca attgtaaaaa
     3661 ataatgagat gattgggacc atttttgctt ctttttattt tcaagatgta aataatttaa
     3721 taagtagaag caattttgat gattctatta aatttctaat gatagataaa gattatacta
     3781 ttatttctca tccaaacaaa aaatatgtaa atgataaaag taagatgctt gatttagaag
     3841 ggaatattat aggaaccact aaaagtgaaa tcttaaaaaa tataaataaa aaatgtcaag
     3901 gtgatttttt atcttgggat aattggagat tatataatgt aaaatatacg aacataaagt
     3961 ggactaattg gactttagta tcaaaatgta atatatttaa gaactttaaa aatctaatag
     4021 ttaattttat gataaagttg tatttttata tagtaatatt tatgatattg tggaaattaa
     4081 gtaatgctaa attaatagaa caacttaaaa aattggctta ttatgattct ttaagtggaa
     4141 taaaaaataa agaaaagttt agaaaagatt ctatgtatat tttaaagaat tattatcaag
     4201 ataattttta cttagtacaa ttggatgtca ataaatttaa atatataaat gagatgtttg
     4261 gatatgctga agggaataag atattaattc atatatcaca ggttttaaat aataatacaa
     4321 ataaatatga aatttgtgct aggatggata atgaccattt tatattgttg attgcttgta
     4381 atactgaaga tgaactttta aatagattat ctaaaataaa taaagaaatt tgtaacttaa
     4441 atactactaa ctcatctaag tataaaattg ttatgtcaag cggaatatat aagattacta
     4501 aaaaggatga tattaaaaag attgacctgt taattgatag agcaaatatt gcagcaaaaa
     4561 gtaaaaagga aaaatatgaa cattcatatt cgttttttaa tgaagataca agaaatcgat
     4621 tgtacaagga aaaacgattg gaagataata tgaataaagc tttagaaaaa ggtgaattta
     4681 tagtatatta tcaacctaag tatagtttag atgatgtaaa tgaaattgaa ggagcagaag
     4741 ccttgataag atggaatagt ccagaatttg ggtttatttc acctatagat tttgtacctt
     4801 tatttgaaaa gaatggtttt attgtaaata ttgatatgtt cgtgtttgaa gaagtatgca
     4861 aaacattaaa caagtggata aataaagggt atactcctgt accaatctct gtaaatatgt
     4921 ctagagttca tttatatagg gataatttta tagaaaatat aactgatttg ataagtaaat
     4981 acaatatatc tcctgagttt attgaacttg aactaacaga aagtgttgtg tttgataatt
     5041 taaatatatt gattgatata atgaaaaaga ttaaagagat aggattcctt atatcaatgg
     5101 atgattttgg ttcgggatat tcatctttaa atctgttaaa agacctttct tttgatattt
     5161 taaaattaga tagaggattt ttaatcgaaa caacagatac aaaacgtgga aaaattatta
     5221 tttctaagat tgtggaaatg gcaaaagcga ttgatattaa agtaatctgt gagggcgtag
     5281 aaacttatga acaagtagag ttcttgaaag agataggttg tgataaggtt caaggatact
     5341 tatttgctaa acctatggta ttagatgaat ttgaaaaaca cttaaacttt aaatttgatt
     5401 aatcattaat aattattaga ctataatata ccctaacaac ttacttacaa atatgtaagt
     5461 taattgttag ggtattttta tggttaaagt agtatattca cgatattatg gtattgtagt
     5521 aaaaataaaa aattatggag aatataaaat gaacatattg agtattagaa atatatcaaa
     5581 aacatattgc ggaaatattc catttaaagc attagataag gttagtctaa atatagaaaa
     5641 gggagaattt gtgtctgtta tgggaccatc tggaagtggt aaatctactc ttttaaatat
     5701 aatatctaca gttgatagac agagtgaagg tgaggttgta ttagatggat atgatgtaag
     5761 taaattgaaa ggagaaaaac ttgctgaatt tagaaggaaa caacttggtt ttgtatttca
     5821 ggattttaat ttgattgaca ctttaactgt tggcgaaaat attatgttgc cacttacact
     5881 agagggggca agtatcaaag atatgaatat acaaactaaa agtatatcta aatttttagg
     5941 aatagataaa attttagata gaaaaaccta tgaaatttca ggaggtcaag ctcaaaggtg
     6001 tgctattgct agagctatta taaataaacc taccatactt cttgcagatg agccaactgg
     6061 aaatcttgat tcaaaatcta ctgatgatgt tttgaaatta tttactagga taaacaaaga
     6121 acaaaatgta acaacactta tggtaactca tgaagtatat agtgcaagtt attcagatag
     6181 agtaattttt ataaaagatg gttgtatata tacagaaata aaaaaatctg aaagcagtaa
     6241 ttctttctat tctgatatac tagctgtttt gtctcaaata ggaggtgtaa gatagtggat
     6301 tttagaaagt ttgcttataa taatatagtt agaaatttca gggcatattt agcatatttt
     6361 ttaagttctg ttttttctat aatgatattt tttgtttttg cagtgtctat gttccatccg
     6421 attataacaa aatctggaat gcagagtggt tctactgcat ttatggctct tatgacttca
     6481 gaattaacaa tattaggttt tagtctactt tttatattgt actctttagg aaatttcttg
     6541 aaatatagat tgaaagattt tggagtattg atgataattg gaatgagcaa cagacaactg
     6601 aaaaaattga tattaattga aaatttgata ataggatttt tagcagtgat gttaggaata
     6661 ttacttggtt tatcaatttc aaaattgttt ttattgtatt taagcaaact gttttatatg
     6721 aatttaactg aaatttattt tccagtaaaa gcaatactat tgactattgt atcatttatg
     6781 attttatttt tgataacagg accaatgagc ttaaaattat tgagaaaaaa gagtatttta
     6841 gagcttttag taggtacaaa aaagcctaaa aaagagataa aatcttcaag gctatttgga
     6901 ataattggtg taatatgtct tatattagga tatgctatga tactatttgg caataagtta
     6961 aaaataaatg gaggtatgta tacaacagca ctattaatta ctttggggat atttttattt
     7021 ttttctcaat ttactatttt gatattaaag tatttaaaaa ataaagaaaa attttataag
     7081 aacaaagtaa atatgttact tataagtaat ttgatatata aattaaagga taatacacga
     7141 ttgttatttt taataacaat acttttatcg gctactttag tagcatttac aacaacttca
     7201 acacttgtaa gttctcaagg agaagcttct aaaaataatt tcccaatggt ttactcatat
     7261 ttttcagagc ataataatga aaaagaatat gaagaactac aacaaattag agaagcaatc
     7321 aaaggatatg attataagga gttaagtttt cctgtactta aatataatag ggaagtctta
     7381 ctaagtgtaa agaattataa caaattatca aaggaattgg gattagataa aattagttca
     7441 aagttagaaa aatatgaagg gattataatt cctagtagca attcacaaga acatataaat
     7501 tctttaaaac aattaaaaaa ttatagtata aaaaatttaa atatagaagt aaaaaaatca
     7561 aaaaataaaa taatttttcc tacaggactg tttagtaaag ttgtagttat aagtgatgac
     7621 ttatacgatg aagtaaaagg tgaattttca aaaattaatt ttcatggatt tgattataaa
     7681 aactggcaaa gcagtgcaaa gataactgaa aatttaaaag aaaaacattt taatttggat
     7741 agagaatata acaggtcata ttttttaaca ttaccagata tgtatttagt agagttacaa
     7801 caaagtaaaa ttcttcaatt tatgggtgtt tttataggag tgatattatt tatagcagtt
     7861 tttagttttt tatacttcag attatataca gacgagatag aagataatgt aaagtacaaa
     7921 aaactatcaa aaataggtct atcttttaaa aatatgaata agattgttag tatagagata
     7981 ggtacattgt tttttatacc gtattttata gcaattataa atagcataat ttctttactg
     8041 tttttaaatc aagtattaaa taatagtttt aggttagaaa atttgggtat attactactg
     8101 atatcatcta tatattcact ttatttttac ttattaaaaa aaatttatac tagaaaagtt
     8161 tggttttttg attaaagtgg tatataatag atatataaat taatatttaa aaagtagtta
     8221 atattagggg agggcatttg cacctccctt gtaatatgtg gaaaattgaa tattagaaga
     8281 aggaggagac ttgatggaaa atcaacaatt attaggaaca gaacgaatta gtaaattact
     8341 actaaaatat tctataccag ctataatagg tatgttggta aatagtttat ataatgtagt
     8401 agatagaatt tttattggaa atataccagg ggttggacca cttgcaataa caggtcttgg
     8461 tgtaacaatg ccaataatga caataatact tgcatttggg atgttgatag gtataggaac
     8521 tactactacc atatcaataa aacttgggca aggtaaagta gaagaggcaa gaaaacttat
     8581 aggaaatgca atgacgttat ctgtaataac aggaattatc ataatgatac ttggaatatt
     8641 atttgcaaat aaaatactta cattgtttgg agcaagtgaa aatactttaa tatatgcaaa
     8701 atcatatata aatattatat tattaggtac agtagtaaat cttctttcct tttctttaaa
     8761 ccactcaata agagctgatg gtagtcctaa aatttcagca ggaataatga tagtaggatg
     8821 tttaactaat atagttttag actggatatt aatatttgga ttcaatttgg gaatacaagg
     8881 agctgcaatt gctacagtaa cttctcaggc tcttacagct attttaacta tagggtatta
     8941 tataagtgga aaatcaaact tacgattcag taaatctaat ctaaaattag ataaaaagct
     9001 aataaaagca gtgtttgcaa taggtatgtc gccatttgcg atgcaacttg cagcaagttt
     9061 agttcaagta atatcaaata ttgcacttaa aactcatggt ggagatttag ctataggggc
     9121 tatggctact atttcttcca ttgcaatggt atttttgatg cctatctttg gaataaatca
     9181 aggagcgcaa cctattatag gtttcaatta tggagctgaa aaatatgata gggttaaaaa
     9241 agcatattta ggttcattag tagtagctac tataatttta tgtatgggta tggtagtagt
     9301 tatgcttttc ccagaagcta ttataggtat atttaataaa gaccctgaac ttatgaatat
     9361 atctgttaat ggattaagaa tatacttact tatgttgcca attgtaggct tatcagttac
     9421 aggaacaaac tttatacaat ctataggaaa agcaaaaatg gctatgttat tgagcctttt
     9481 aagacaagta attttattga taccagcagt attaattctt cctacattct taggtttaca
     9541 gggtgtatgg acagctcaac cagtgtctga ttttatagct acagtaatta caggtatagt
     9601 tgtatttaga gaattaaaaa gatatactcc taaaactgaa aaattaaatg aaaatgaaag
     9661 actaaatgag ataactactg agtaaaaata taaattcaga taattgagca agaaattgca
     9721 aaaaatacga ttatatgata aaataaaacc gctgtcaagg ataagagaaa ttctaattat
     9781 ggagtgattt tatggaattt acaaagaaaa gcaaattaaa aaatatgtat gtaaacatga
     9841 atgtagctag aatgttgtct aagataaatg agtataaggg taggcaactg ctttacaaaa
     9901 aacaaccaaa agaaatttta gaaaacctag aaaaaaaatc tttagtagat tgttctgaat
     9961 ctacatacat tggaaatcaa agagatagta gtaattttaa tctagaaaag ttgataagta
    10021 atgaagttac acctagaagt agagaggaat tgagtattgt tgaatacaga gatgtggtaa
    10081 aaactattaa cagtgcttat gagagtattc ccatcagttc acaaacgatt cttgagcttc
    10141 atgggtactt atataaattt tcatctacta gaggaggaag ttataagtca gataatgatt
    10201 ttattgaaca caatttaaaa cctagcgaat ttataagtat tgattcaagt gtcaataaag
    10261 ttgaaaaagc tgtagaagaa atatgtgaag cttataatac attgatagag gaagatgaaa
    10321 ttgatatatt aatattaatt tctgcatttg tgttagattt catattgata catccattca
    10381 aagaaggtaa tataaaaatg gcgagagtac ttatcctatt gttactaaat aaaaatgggt
    10441 atgaagttgg aagatatata agtttaggaa agatatttga tgatagttca tatgaatatt
    10501 atagtaattt aaattatttg aaagcctcaa taggtagtga aaaagcagat atgaatgcat
    10561 ggattgagta ttttttagag acgatattaa ctgcatatga gaagctggat gatagtttaa
    10621 atatatctga taagaaaaga caaacaaaaa ctagtagaat agaaaaaata ataaattcta
    10681 cccttggata ttttacaaaa gaagatataa gagatttatg tcctgacatt ccagaaccta
    10741 ctataaatag agtttttaat aatctaagaa aacaagataa gatagaagtt gttgcaagag
    10801 gaagaagtgc aaaatggaag aaaaaatatt aaaaaatgtt taaataacta aaatggtata
    10861 gtgttttaca ctataccatt tttaatgtca ttagcatgag caataattta acaacttaag
    10921 caatatcaat tttatttatt gattattttt gaactgaaaa atggggttat atggtatact
    10981 acaataataa ttaattttgg atgagaatat ataacgttac agaatatctt aggagtatat
    11041 gtataaaaaa tataataaaa gtgtgaggtt tagtaattaa caaattatgt ggcttcacac
    11101 ttttttataa aataaaaata cttagcagaa gaatatttta gttgatatgt ttataagata
    11161 gtacttgttt gggtaaaagt ataaatagta aaaaagtaga agtaatgtgt ataaaaacat
    11221 aagttacatt ctgtaataat agttatttta taattcaaca aaagaataga atgggtatat
    11281 ttgtgaaata ataatataaa ataatcttag gagtgatctt atgaagaaaa aattattata
    11341 tataaatgta aattcaaaac ctgaagactt atcttcaagt aaaactgtag caagaaaatt
    11401 tataaataaa tttatggaaa aaaacaaaga ttttgaggtt gaagagatag acctttataa
    11461 agaacatata ccgagacttg agtatcagta ctttgaaaaa agaaatagca tagttagtga
    11521 agaaaatgct aaaaatttag acgataagga tagaaaagag cttacaaaga ttagaaatct
    11581 ttgtgaccaa tttgtgagtg caagtgttta tgtaatagca gcacctatgt ggagtttatc
    11641 ttttccagca ccacttaaag agtatataga ttgtataatt caagatggaa aaactatatc
    11701 ttttgaaggc aatgataaac cacaaggaat tttaaatgat attgatagaa gtatggtata
    11761 tatacaatct tctggtggac acataccatg ggtcttgaag ccagttatga ataaagggtt
    11821 aaattatgtg gaaagtatta tgaaatttat aggaattaaa aaatttgatg aattgttagt
    11881 agatggaact ggaactagtg aagaagaaag acaagctgca atagaaaaag catctgaaaa
    11941 aatacatggt ataatagatg gtatgaaatt ttaattataa tattttatta aaaatataaa
    12001 ggaaaaacct cttttaatgt agaaacttat attgaaggag gcgagaatca tgcgagaaga
    12061 aaaaagtaat gaaaagtatg attgttattg gtgtaatcaa gaaaataatt tctgtgtaga
    12121 aataaaagat aatatagtaa tgatagatga tggcactggt acgttaaaac aagccgtttt
    12181 catagggtat aaacaaatcc aaattaattt aaattgttca cattgtcaaa acttaaatag
    12241 aataaaatta aatttgtaga attttaaaat ggaaattatt gaaggaatta aaaatagaaa
    12301 gtgttaatat ttagactgaa aattatattt tatacaataa aagtttattt aaataacatt
    12361 gtattttaat atttattttc agtcttactt tattaaaatt attttttggt tatgtctgga
    12421 aaatatctgt ctactaattt ttctgaaata ttttttaatt ggtcagccat tgaagtcaga
    12481 cttaataagg ctatcaataa atcatcttta ttttctggat ttatactaat attttcaaaa
    12541 ttagactcta taaagtccgg tatattcttt ctttgatttt cttttattaa catgaatttt
    12601 tcgtaacatg aaactaaatc tttttcactg aaattatcct tttcatacac accatgtaag
    12661 attcttttaa tttcttgtat tgtaagtgta tttttcatag aataaataat tatcatctgt
    12721 aaaatttgtt ctttagtata cttttttccc ttaactggtt tgattaatcc ctctttgctg
    12781 taattattta tcatggtttt agtaagaatt ttatcacttt caaatctttt attaggagaa
    12841 agtttattgt caattagaga aataatttga tccatatata agtcaataga gggaatatcg
    12901 tttgaagtta tatctgcatt atttagtgtt tctagtatta tagtattaag ttcattattc
    12961 atcatatgtt acctccataa tatatataat acactaaatt agaaagtaaa acaatattaa
    13021 gacttgtaaa aaaaacataa agtggtataa ttaaatatat aatatagtaa tgaaaactat
    13081 atttatgaat ctgattttta aaatacataa ttgtaggagg acgaaatgta tcaatttttt
    13141 ttaaagggta gagacccaat aagcagttta actcatttta taggtgcttg tttatctctt
    13201 ttagcaacta taattttagt ttttcaatct gttacattac aggaaacttc tctcttgatg
    13261 atagtatctg tatcggtatt tggactttca ctaattgctt tatatagtgc aagttcgtat
    13321 tatcattttt taaaggggac accagaacaa gaattgtttt ttagaaaagt agaccatgca
    13381 atgatttatg tattgattgc tggctcttat acaccaatat gtctgaattt tatggaaaaa
    13441 aaagaaggca taatatttgt tacagcaata tggatagttg catttattgg cataataata
    13501 aaaatatttt ggatggatgc acctagatgg ttatctacca gtatatattt acttatgggg
    13561 tgggcaattg tttttgatat aaatgcattc aattcaattc ctaaggattg ccttagacta
    13621 ttaattatgg aaggtgtatc ttactctata ggtgcaatta tttatataat taaaaagcct
    13681 aatataagtc ctgagtttgg atttcatgaa atatttcata tttttattat gataggttct
    13741 ttatttcact ttttagcagt tttattgtat gttttatagt tttaaaagat ttgattaatt
    13801 gatactaaaa ttctacaatg aataaaatgg aactgcctta tatagattat atcttcgaca
    13861 ataagacagt tccttattat aaatattaaa ttctacttat aataaaattt tgataaaaat
    13921 agtccattca aaatgcctat tccataattc ctttaatgta cttttcattt acgacatata
    13981 gtttgaattt atgtactata attatatcct ctaaattctc tgaaacacta acaattctga
    14041 cacatttgtt tctaatttct acaggtaaat ctaaatcatt tgttaaattc acatagtcat
    14101 catatttata cataaaatca tccct
//
LOCUS       DAEPOG010000103        14104 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEPOG010000103 DAEPOG010000000
VERSION     DAEPOG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.103"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            444..1514
                     /locus_tag="KR432_002445"
     CDS             444..1514
                     /locus_tag="KR432_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0640973.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KR432_002446"
     CDS             1498..2367
                     /locus_tag="KR432_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0640974.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KR432_002447"
     CDS             2411..3271
                     /locus_tag="KR432_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0640975.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KR432_002448"
     CDS             3302..4606
                     /locus_tag="KR432_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0640976.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KR432_002449"
     CDS             4669..5931
                     /locus_tag="KR432_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBH0640977.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KR432_002450"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KR432_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBH0640978.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KR432_002451"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KR432_002451"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0640979.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KR432_002452"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KR432_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH0640980.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KR432_002453"
     CDS             13008..13937
                     /locus_tag="KR432_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBH0640981.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5748 a         1618 c         2550 g         4188 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggtccaagt ggatgtggta aatctactac attaagaatg attgcaggtc
      601 ttgaagatat aacttcaggc aagttaaaaa ttgaagaaaa tgacgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaaatc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taagaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagcctatt tggaaaagtt gtgactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagaa ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatacaa tatcttaata gtattatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccgc tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataagat ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatggaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcga catcctttaa aaatccacaa gaaatgatgc aattaccacc
     2581 tactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttactaa acagtgtatt tgtaacagga agtgttttaa ttttacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gttatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat attccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aactattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataga gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattacgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta aattctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcctacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4921 aaggacataa aagtaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagctc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatattg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 gatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatata
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaagggcaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgagaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaaggat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc gcaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat ggactttaac
    11101 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttggaccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaaacaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagaggcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatacgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaa
//
LOCUS       DAEPOG010000104        14077 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEPOG010000104 DAEPOG010000000
VERSION     DAEPOG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.104"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            202..1008
                     /locus_tag="KR432_002454"
     CDS             202..1008
                     /locus_tag="KR432_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0640982.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="KR432_002455"
     CDS             1135..1407
                     /locus_tag="KR432_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640983.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="KR432_002456"
     CDS             complement(1570..2142)
                     /locus_tag="KR432_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0640984.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="KR432_002457"
     CDS             2326..3168
                     /locus_tag="KR432_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0640985.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="KR432_002458"
     CDS             complement(3271..4347)
                     /locus_tag="KR432_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0640986.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            4844..6247
                     /locus_tag="KR432_002459"
     CDS             4844..6247
                     /locus_tag="KR432_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0640987.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    6338..6578
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6621..7319
                     /locus_tag="KR432_002460"
     CDS             6621..7319
                     /locus_tag="KR432_002460"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH0640988.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            7342..8343
                     /gene="trpS"
                     /locus_tag="KR432_002461"
     CDS             7342..8343
                     /gene="trpS"
                     /locus_tag="KR432_002461"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH0640989.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(8995..11625)
                     /gene="cdtB"
                     /locus_tag="KR432_002462"
     CDS             complement(8995..11625)
                     /gene="cdtB"
                     /locus_tag="KR432_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBH0640990.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(11678..13069)
                     /gene="cdtA"
                     /locus_tag="KR432_002463"
     CDS             complement(11678..13069)
                     /gene="cdtA"
                     /locus_tag="KR432_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBH0640991.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(13459..>14077)
                     /gene="cdtR"
                     /locus_tag="KR432_002464"
     CDS             complement(13459..>14077)
                     /gene="cdtR"
                     /locus_tag="KR432_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBH0640992.1"
                     /translation="LKELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYIS
                     PIIFLTNHIEMILGIFDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSN
                     FFTIYSDSSLWKISFDEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRV
                     HKSFVVNKYHIVSLDLKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         4925 a         1833 c         2096 g         5223 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtattcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agtaataaat taagtctata aagttatgta
     4561 ttttatcttt ttatatacta atgaaaaata ataaataaaa ttttataata aaataatatt
     4621 gaaacaattt taaaaattta gtatcattag aattaaagtg gtaaaacttt taaatttatc
     4681 atttttaata agatttgatt tcaaagtatt taaggtttat tattaaaact gtttttgaat
     4741 atgcaaatat tttgaaaaat atcagtttaa taataatgaa tctattttat taattttgaa
     4801 attaaattta aaataattaa tagagagggg aaatgaaatt aggatggaaa agaaaaaaat
     4861 aggcctatgg agtttggtat ttatgaatgt atcagcttta tatggtataa gatggatagc
     4921 taagtctaca gcatcaagct ttggtttagg attaggtgca atacctatgt ggttattatt
     4981 ctcatttata tactttgtac caggagcttt aatttgcgct gaattagctt ctacatatca
     5041 aaagggtgat ggaggtcttt acgattgggt taaggaagcc tttggagaaa agtatggatt
     5101 tttagtttct tggttaaact ggacagctaa aatattttgg tattcttcat ttttaacttt
     5161 cttagcagta aatgtttctt atacaatagg aaatccagct cttgctgata ataagatgtt
     5221 tgtacttata ttatctttaa taatattctg ggcattatct cttatagcaa ctaaaggaat
     5281 aagttttaca aaaatattta ctaatacagg tgcattaggt tcaacaatac caagtgctat
     5341 acttataata ttttcatttg tagcagtatt tatgttgaaa aaacatgata tagcttctac
     5401 atatacaata gcttcaatga ctccaaaact taatatggat gcatttgttg ctatatcagc
     5461 tataatgttt ggtttagcag gagcagagac tgctgctaac ttcataacag aaatagataa
     5521 gcctgaaaag aacttcccta aggcaatatt aatatcagct ggaatagtag cttcattata
     5581 tgtattaggt tcaattgcaa taacaatgat aataccacca gaccaaatta ctgcttctaa
     5641 aggtgtatta gatgcacttt ctgctgtttg tacgtcttta ggaattggtt cttggttggt
     5701 tcaattaata gcttttggta tagctttctc agtattagga gcaatagttc tttatatagc
     5761 atcaccaata aaaatgttat ttggtagtgt taaaaaagga atatttccag atagtttggt
     5821 agaggtaaat gaacacaaga taccttctaa agcagttata cttcaagcaa taatagttac
     5881 tataatacta ttggtgacta cattaatgcc ttctgttgat gctatataca atgttcttgt
     5941 aactatgaca gcattaactg cattattccc atatgtatta ttatatgctt cttatattaa
     6001 actaagaaaa gagagaccag atgaaattag accttatact atggcaaaat caacaagtac
     6061 ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta gttggtatat tattatctgc
     6121 agcaccagtt atgccgacat ttgctgaaaa tgttatatat gagatagaga tgattggtgg
     6181 aggtcttcta gttatattat ctggtctatg gttatggaat agatatgaaa agaaaacagc
     6241 taaataaaat gtattttaaa aatttctttt ggatattata atatatagtt gacaatttat
     6301 agtgtaagga atattataaa ataaagaaat taataaatta aatcctgaga taagaaatag
     6361 taaataaata tatttttgca cagagaatta aggatgctga gaacttaata aaaagtattt
     6421 attgaatggg tctttgagga gtaaaatgaa atgtaattta agattacaga gtatttgata
     6481 tcgtgtatgc acgttatagc atatattaag atatatatat tcagttttga gtgtatataa
     6541 atttaggtgg taccacggaa aatatatccg tcctattgat ttaggacgga ttttttaata
     6601 tagaaaattt aggaggaaaa atgaacgtaa taggaattat aggtgcaatg gatgaagaag
     6661 ttagtatttt agttgattta atggatatta gagagactat aaaaaaagct agcttagaat
     6721 tttataaggg aattttgaaa ggtaagaatg tagtgttagt aaagtgtggt ataggtaagg
     6781 ttaactctgc tctttgtgcc cagatactaa taagcgaatt taaggttgat gctattgtta
     6841 atacaggtgt tgcaggagct ttaaatgaaa aacttgatgt taatgatata gtaatatcaa
     6901 ctgatgctat acagtatgat gtagatacta cagcatttgg agaccctaag ggagttatac
     6961 caagaatgaa gacttctgta tttaaagcag atgaaagact tatagatgct gcttacaaat
     7021 cttctgtgga agaagtaaag actcataaag tattaaaagg aagagtagtt acaggagata
     7081 aatttataaa ttctaaagaa ttaaaagaag aattagtaaa tgattttggt ggatattgtg
     7141 gagaaatgga aggtggagct atagctcatg tatgctattt aaataatact ccattcgtaa
     7201 taataagagc aatgtctgat aaggcagatg gaagtgctga tgttacttat gatgtatttg
     7261 tacatgatgc tgccaataac tctaaagaca tagttttaaa tatgttaaaa tcaatataaa
     7321 tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta tatttagtgg agcacagcca
     7381 tcaggcaaaa tgactttagg aaattattta ggagctataa aaaactggac tgaacttcaa
     7441 gataattatg attgctatta tagtatagtt gatttacatg caataacagt acctcaggac
     7501 cctaaagttt taagagcgaa tgctatagaa ttacttgctc aatatatagc ttgtggatta
     7561 gacccagaaa aaaatactat atttatacaa tcacatgtca aagagcatgt tgaacttatg
     7621 tgggtattga atactatgac ttatatgggt gaattaagta gaatgactca attcaaagat
     7681 aaatcacaaa agagtgaagc aaatctaaat gcaggtttat tcacatatcc agttcttatg
     7741 gccgctgaca tattgttgta tcaaactgac ctagttccag ttggagatga ccaaaaacaa
     7801 catttagaat tagctcgtga tttagcaaat aggtttaata atagattttc acctactttt
     7861 gtagttccag aaggatacta cccaaaaggt ggagcaagag tgatgagttt gcaagagcca
     7921 actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat ttatactatt agcagatgat
     7981 tcagattcta taaagagaaa gataaaaaga agtgttactg attctttagg tgttgtaaaa
     8041 tacaatgatg aacaaccagg tattaaaaat ctaattgata tatattctaa tttaagtaaa
     8101 aagagtgtag aagagattgt aaacatgtat gaaggaaagg gttatggcat atttaaagaa
     8161 gatgtggctg aagtagtatc agaagcttta agacctataa gagaaaaata tgttgacctt
     8221 cttaataata aagattattt agaaaaaata tatagtatgg gagcagaaaa agccgaaaaa
     8281 caagctagaa aaactcttag aaaagtttat aaaaaagttg gatttataga gagaagatac
     8341 taattattag ctatctttac ttgataaata aattttaaag ttattaaaaa ggtatatgat
     8401 atgaaagaga gagtctcaaa gtgaactttt gttcatgaga cactcttttt tgtatgaaat
     8461 aaaatatatt ttaatcactt caacaatgaa aaagaggatt atctctattt tgagacagca
     8521 tcttttgata ataaaaaaag atgagctttt gctcatctca aaggggttag attattctta
     8581 ctttggggaa agtagaataa aggtaaaagt taactgatgt caactttgtc ttttttgcat
     8641 atcactactg agcataaaaa aatcagaaaa ccatagcctt ctgaaacaac ttctaacata
     8701 cattgtattt tatacccaat atgtaattta tgagttaatt ttaactctta taatggaaat
     8761 tgtcaataat atatcaaaaa aatatttttt aaatttatat aattaaatga atttatgttt
     8821 atatataggt gatatgatac agataattat ctttataata caatatagtt ctaaaaaaga
     8881 atatgttaga agaagagaat atttaaataa aaaatacctt ataaagttaa ttttttagta
     8941 ctttataagg taaatatata ttgtattgtg gggacaaatt taaatcattt gatactaatc
     9001 aacactaaga actaataact ctctatcgtc tggagtaatt gcatatattc ttaatttctt
     9061 gtatgtcata atattttctc cacctgtaaa ataactccta agattaacat aattagtttt
     9121 aggctgattt ggatctccta aataattacg catttcttta tctttagttc caatatcttt
     9181 aaatccagaa tattgtaaag tagcttcaac tctatatttt tggatataat cgagagcttc
     9241 tttattagtt tgtgttggtg caaaatacat accatttata taagtatttc ctgtacttgg
     9301 attaaaattt aaatctgcaa aatatatttt atgtgcatcc attatttctt ggtcagttgg
     9361 aattttaact tctggttcat caagtatttc aggagtacta tttagctctg taatagataa
     9421 gttatctaag tatgttgtac cactaccaat taatgttatc tctatattag aagaatcttt
     9481 ttcagtagtt tcaaattcat aactaaattt tgtatatcct tgttctggta ccaaataatc
     9541 cgttttctct tcttttgctt ttatctttac aattattgaa ttagatgtta aaggatcctt
     9601 tgaatatcca ctaaaaacat aacgtttata aggttttagc tcagacatag ggattttaat
     9661 cttcgtctca ccatttaatt tatttgctga gccttgtaaa ccatcttgat tcgtagtatt
     9721 gacattactc catgtactag ggtaattatt ataatcatca aaattagtaa agtaagttgg
     9781 tgtttttata agtatattca ttcctctttc aagtttaaca ttatatatct ttttatcaga
     9841 caaagttttt aaactatctt taatcttatt ggctgtatta tcatcaaata tgagttcaac
     9901 acaactttca tctattggta tatcattaaa atataacaaa ccatcttttt tagtagcgcc
     9961 aaaagctttt tcaattgctt ctccaattgt aagttcaggt gttttatctt ctggatcctg
    10021 taaattttta gcagtaactc ttctttcgta agattcattc tctgtatcta atataataga
    10081 tgcagaaata ctgtcaattt gacttatata gtctgaccaa ctatttcctt ctgttactat
    10141 ttgtccagaa ctattttttg taccaaaatt tccacttact tgtgttgttt ctaatttaat
    10201 ttgctttcca gcatctaatt tttttaattg atcataattt ataggaatca gtctagagct
    10261 aaattgatcc attgtgttaa gagctagagg tgaaagccct tttttgggat aagtatctcc
    10321 aggagataga ttattgccaa tttgattttc ttgtgctttg atagttgata atgtatctcc
    10381 atctaacact aaatttgttg ttggtgtcac tttgtacata ggtgcagtac ctgtgttgta
    10441 atatctaaca tttgcattta tatatgcaga ttctcctttg tttatactta atccagtatt
    10501 ccatgattct ccattactat cttgaacagc agttgaatta tctgttgtat gggaataatt
    10561 tgtagttaca ttagctgtga atccattttg atatcctaca ttaacagaca caccagctgt
    10621 attagattca gttttactgt tagtagtagc tctggaaaca gttttacctt gatcagtaga
    10681 ggcatgttca tttgtagata taattaattt ttccatacct actccaacaa ttggatatgc
    10741 tgcaactaac ggatctcttg cttcagtctt tatagccttg tcaaaagaac ctgaagcttt
    10801 ttcataatct gtatatggat ctccagcagt atttgactct aaataatttg atacatattt
    10861 cttatagcct tgttctgcaa aactatcttc ccacttaact gcaattaagt ccttaatagt
    10921 atatccattt cgttcatatg aatctggtat attatcatta tctgtatcca aatcttcgtc
    10981 ttcccaatca gacatcaact ttgggtcaaa gaaattgtta tttgggataa atggatcatc
    11041 tttttctata ttagaataat ctcttaagaa taaattttct tctggtataa ttttcttcat
    11101 accatctaat tcccaataaa gattaggtga tgataaatta tctattgaac ctaaattttt
    11161 atcttgtagc tctattctaa ctttatattc tttacccttt ttcatattaa ctttaagtgt
    11221 atttgatata gtactctcag tatttacttg cattaagaca tcatctctat cagttgataa
    11281 tgtatattca ccatccttag aaggaattat tcttcctgtc catcgtatag attttacatc
    11341 tgatttgtct ttatccagaa gtttatctac tttcttttct tcaaatttta aattaccatc
    11401 ttttatgggt gccattaatt ttaaatcttt aaagtgctca tctgtgaaat aatatcccat
    11461 taatccattg tttgggagta tatcttcatt tacaatttct tttttcttat tagaatgatt
    11521 acttgtacta gtttgagcat atacaggata tactagtgct tgactaacta tagctgtaag
    11581 tgttaaaaaa cttaatacct ttttattcct catttgtatt ttcattgttt ctcctcctta
    11641 aatttgaaaa atattaaatt atttatgtga aaaaatatta aggtatcaat gttgcatcaa
    11701 caattaattt tgttatagta ccatctttgt aagaatcaat tttattgatt ttaaatttgc
    11761 ttccatgatt taaaagcact tcatattcac ctgcataacc tggaatagct gatagataag
    11821 ctccaggaga acctttaggt atagttatac gtagtactat ttttctttta gcaaatgcac
    11881 tcatattcac actaccaata ctagtactaa taaagtttgg ataagacagt gcttgtcctt
    11941 cccattttga tttaaaagca tctatatttt ctagtttgtt aaaatcatat tcaggggaag
    12001 taagagttaa accaaattct tgaggaccag atcttctata tacagttaaa ttagttggaa
    12061 taggttcacg ttttaatgca ttttcaatgt ttgtgatttt agaatctaat tctgggttag
    12121 gattatttac tggaccattt gatattaaat aattattaat tgcagtatat cctccacgca
    12181 tataatcatt tacatcagca agttcattag gtgttaattt attactccaa tcattataat
    12241 ttgctttacc ccaagaatcc cccttactta catcatcttt aaaatcaaga ctacttacaa
    12301 cagatgcttc tgctttaata tagtgcttcc catctataac tatacgaaca attttatcta
    12361 tttttatact atatccttgc tctattaatg tacttacatt gttagtattt gtatatggta
    12421 acataccagt atttctaggt aattttaagt gcataagtaa tggtgtaggt ttttcatcac
    12481 cttttccagg ttcatataaa gaaatatctt taaatccatc ttgcttaaat aatttgtttt
    12541 gtatagtttc tttaaactca ttaaattttt ctaatgaaat ttcgttttga ttttctgttc
    12601 ttattacttt attaaatgca aatttttctg gagattcaaa ataatagaca tacataggtt
    12661 tatctatttt attttttgat atagcatttc gaagttcttt atattctttt tctcgagaat
    12721 ttgcttctat ttgataatca taaaaataat ttcttgtctg agaatattta cttatttcta
    12781 cagaatcttt tttatatgat tctaatgctt ctttttcaga tctttcaagt ttttgctcta
    12841 ttctttctgc ttcttttctt tcccattctt tagccttttc tttatcttta agaaaatctt
    12901 ctggtctttc tataggagcc ttgtaagtag tattgcaaac tttttcacta tagctttgta
    12961 agtcttgtgc ataaatgtta ttaggtaaca aaccacctaa agttagatat aatatcaaca
    13021 atatagatat acaattacta atccttttat gtttcctaaa ttttttcatt tattctccct
    13081 cccaatatta gtttaattaa ctcttgaact ttaatttatt tagattatat aatctaaata
    13141 agaatttttt atataaattt cataaaatat gtaactatat atcataaaaa tatgtattta
    13201 tatgatatgt cagtacaaaa taacaactga atattttaaa gagactattt catactttta
    13261 tgatttaatg agctatcttg ttgaatcata atctaggaac aatggttgca atgtctaata
    13321 gaaattgtat atgatatagt cattatgtgt tttttcacaa aacgattatt atatagagga
    13381 ttcttaggaa ctactttttt tgtataaaaa aagagatggt tcaaattctt atcttgagac
    13441 atctcttttt tctatttatt atgttttaat aatgttcttt aaaatatttc tatatgtatt
    13501 agatatacga catatatggc cattactcat tttaatatta tttttcttta ggtcgagaga
    13561 aacaatatga tatttattaa ctacaaaaga tttatgtaca cgaataaagc atgcatctaa
    13621 atctgataaa gaccttaaac ttttatagaa ttcaaatatt cttgaagtag tcactaattt
    13681 tattttatgt ggaatagcgc ttgtttcaaa atatataact tcatcaaatg aaattttcca
    13741 tagtgaggaa tctgagtaga tagtaaaaaa attagagcga taattttttt ctttaacgta
    13801 tcttttatga gcaattttaa tacaagcttt tattttactt tcagcaattt ccatatcatg
    13861 ttttaatata aagtccataa cttctaacct ataatcgaag atccctaaaa tcatttctat
    13921 atgatttgta agaaatatga ttggactaat ataatctgat tctctaattt tttgtgctat
    13981 ccataatcca tcacataatt cattacttat cttgtatttt gcatctacaa ctataaaata
    14041 tatttttact ttattttttt cagtaagttc ttttaat
//
LOCUS       DAEPOG010000105        13755 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEPOG010000105 DAEPOG010000000
VERSION     DAEPOG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.105"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(136..768)
                     /locus_tag="KR432_002465"
     CDS             complement(136..768)
                     /locus_tag="KR432_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0640993.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            1574..2341
                     /locus_tag="KR432_002466"
     CDS             1574..2341
                     /locus_tag="KR432_002466"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH0640994.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2669..3346)
                     /locus_tag="KR432_002467"
     CDS             complement(2669..3346)
                     /locus_tag="KR432_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0640995.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(3496..3813)
                     /locus_tag="KR432_002468"
     CDS             complement(3496..3813)
                     /locus_tag="KR432_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640996.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(3835..4173)
                     /locus_tag="KR432_002469"
     CDS             complement(3835..4173)
                     /locus_tag="KR432_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0640997.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            4860..5426
                     /locus_tag="KR432_002470"
     CDS             4860..5426
                     /locus_tag="KR432_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBH0640998.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            5731..6192
                     /locus_tag="KR432_002471"
     CDS             5731..6192
                     /locus_tag="KR432_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0640999.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            6260..8506
                     /locus_tag="KR432_002472"
     CDS             6260..8506
                     /locus_tag="KR432_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641000.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            8499..10331
                     /locus_tag="KR432_002473"
     CDS             8499..10331
                     /locus_tag="KR432_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641001.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            11050..12327
                     /gene="hflX"
                     /locus_tag="KR432_002474"
     CDS             11050..12327
                     /gene="hflX"
                     /locus_tag="KR432_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0641002.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            12560..13057
                     /locus_tag="KR432_002475"
     CDS             12560..13057
                     /locus_tag="KR432_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0641003.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            13107..13631
                     /locus_tag="KR432_002476"
     CDS             13107..13631
                     /locus_tag="KR432_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0641004.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         5279 a         1452 c         2196 g         4828 t
ORIGIN      
        1 tattaagtaa ctttactttt tattttattt aatgttgtat taaatttata tatattaaaa
       61 taatatacaa attttgaaaa agctcttata aaaggattat taaagtaaag tttaatataa
      121 ataaattata aaatattaaa ctaatttatc aatatttcct gattgaatat gcttaatgtt
      181 tgattgaatt tttttatatg gccaatccca ccatttaatt tctaataatt ttgaaattgt
      241 atcattatta aatctctttt ttataatttt tgcaggtact cctccaacaa tagaatacgg
      301 tgagacgtca ttagtaacta ctgctcttgt tccaataatt gcaccatcac caatatttac
      361 acctgacata attatagaat cataacctat ccacacatca tttccaatta taatgttacc
      421 cttattgtcc caagcgtccg ttatatggtt gatatcgagt ttccattctt cataaaagat
      481 gggaaaagta tatgtggatt ttgatttcat tgagtgattt ccactagtca ttagaaattt
      541 tgcattacat gctattgagc aaaacttacc aattattaat ttatcattgt ttatagggta
      601 atgatataat acattgtttt tctcaaaatc tcttgggtca ttataaaagt cattatatat
      661 tgtgtaatct ccaacttcta tattttccct tgttattaca tttttaagat atacagtttg
      721 ataatcatta cttcttggat ataccttatt tgaatttgga atagtcatat ttaaatctct
      781 cctttaatat atattatgtt tttgactatt ccactactac aatacatctc ttcacagaat
      841 cactccttga tagtatctat attttgattt ataatttata catttttttt taaaacagac
      901 ttttaactgt atagtatttt ataaagatta tatgtttagt tgattgtata tcaaaacaat
      961 aaaacttgca acaatttaat atattttata tgtaaacttt ttctaattaa cctaatgctt
     1021 tatcaacata ggttagaaaa aaacttccgt atttagtgag ggaaacattt ctaccttgtt
     1081 tttcaaataa aaagatacca aattcttttt caagaatcta tataacatta cttaagcttg
     1141 gttggggtat aaacaattga gtagctactt ttgtatggtg ttcaacttta gtcaaaactc
     1201 tgaagtaatg caaatgattt agattcatta atattcaatc tctttataag tagtgaaatt
     1261 cgagttattt ctgcataatt atactatgat ttatagatta attctattag catccagaat
     1321 aatattgttt atatgagtaa aatttaaaga atattgtgaa aattatagaa tgttaaaata
     1381 aatttaggta aaaaagaagt gtttgtatat tatagagtac cttttctaaa atatgtggga
     1441 ttgttttaag aattagaata aaaattattt ttaaattagt atttttataa tgtatagtat
     1501 ttttaatgta taattaaatt atgcagaaaa tagaatttaa agtcctatag aatttctgat
     1561 tatggaggag aatatgaaaa gaaaagttca agtaaaaaat attactattg gagaaggtag
     1621 acctaaaatt tgtgtaccaa ttattgggaa aaataaaaaa gatattataa aagaggctaa
     1681 agagttaaaa gatgcatgtt tggatattat agagtggcga gttgattttt ttgagaatgt
     1741 agaaaatatt aaagaagtta aggaagttct ctatgaatta agaagttata tacatgatat
     1801 ccctttatta ttcacattta gaagtgtagt agaaggggga gaaaaattaa tatcaagaga
     1861 ttattatact actttgaata aagaaatttc taatacaggt ttagtagatt taatagatgt
     1921 agagttattt atgggagatg aggtcataga tgaagtagtt aattttgctc ataaaaaaga
     1981 agtgaaagta attatatcta atcatgattt taataaaact ccaaaaaaag aagagattgt
     2041 atctcgttta tgtagaatgc aggaattggg cgcagattta cccaaagtag ctgttatgcc
     2101 gcaaaatgaa agagatgtgt tagtgctact tgaagctaca aatgaaatgt ttaaaattta
     2161 tgcagataga ccaataataa caatgtcaat gtcaggaatg ggtgttataa gtagattgtg
     2221 tggtgaaata tttggttctg cattgacttt tggagctgca aaaagtgttt cagcaccagg
     2281 gcaaatatca tttaaagagt taaattcagt acttaatcta ttacataaga gtattaatta
     2341 aattaattat atagaaatat tagacggtat ttttgaagat ataaattaaa tataaaaaac
     2401 ttttcagata gtaaaattta aattgggcaa ttatatctga aaagcaacta tttagtgtgt
     2461 attttgaaag tgatatagaa aatcttataa atcaactatg gcatatttaa aatatgccat
     2521 taaaacctac tagacattag attatagatg gtatactagc atttctatta tttatagata
     2581 gtaagttttc ataataactg ttttatctaa agtagaagat atatttgtat tatgtatatt
     2641 tgtcattatt tttttgtaat tatgtatatt aatggttata tatgtaatat aatattatca
     2701 ctctattttt tgtatattgt acattacaac atttatatct atcatcatca attacagtag
     2761 aatgtatggg atacttattt tcacttaacc aatatttttg catattcttt gtccaagact
     2821 ttggaaactt caatactttc ttgtttatta taacatcctc atcttcaaaa atattaactt
     2881 ctctttctat ttttattttt ctgttgtata tcttattgat agattctttg tcgagtttta
     2941 tatctctata catatcaggg caatagcaac aaacataatt tctttttttt cgacgtttaa
     3001 atttcacaac tttctctatg ctaatttcta atttttttgt accatctttt ataaatttaa
     3061 tatcttctat gttctcgctt gaaaaaattt ctgtcaatct taaagtatct acagtttctc
     3121 taattatttg ctttttataa gaagagaatc catatccaaa agaaaggttt aatttattag
     3181 ggatgtagcc atttttatat ttaaacatct taattttttc caattcagca ttatctttaa
     3241 atttcaactt ttcataccta aaagttactt tgctaaaaag agtatattct acatcaatat
     3301 attcttttgc aatcttaaat ttatcactac cttcaatcac aatcatagcc tatctccaat
     3361 cgttttatat taaaataagg ccatagaaca tggtctaata gtactcatta gcactataag
     3421 acaatgttaa cacaaaaaga caaaaaaagg ttatttattt caaataacct ttttttttat
     3481 ataaaatata aagaattata ttttattctt ttttatgatg tctataatct gtgatatcag
     3541 atttagttct cttgaattta agtcttttaa ctcttcattt atatcatttt ctaaatatgt
     3601 ttttccagtg gaagtaatat cttttataag aacatctgca gaaatattta atatatttac
     3661 aaattttatg aatgttttaa gacttggatt ctttttacca ctttcgattt gagttatata
     3721 ccaaggcgat actccaacca tatttgcaaa ttcttttgaa gttaaatttg ctttttttcg
     3781 ttcttcttta agtcttttac ctatataatt cattattgtt actatttccc tcacttatat
     3841 ttctttgtat ttaatgatgc actttattaa ctctagcatt gcaatttgtt ctttttcagt
     3901 taatttatta atcatttcat aacattctaa aggtacagag ttatcaacgc cttctgttgt
     3961 aataatattt gcaaataaca attcaagtgg tatgtctaaa gcagtagcaa ttttcattat
     4021 aatattgata gatgtatttg tctcagctct ttcaatctga ccaatatatg ttggatgtaa
     4081 acctgccttt tctgctagaa actcttgact atatcccaat tttgttctat agacttttat
     4141 tctatctcct attatttttc ctatatttgc caattattat ccctccactt tagattatat
     4201 tcatagttta agttgtttta tatagactat ttatactttt ttagtctaaa tatagactat
     4261 atatgttttt atactgaaat aagtgctatt tatttttgta tagtctgtat ctacaataaa
     4321 caaatatgga taaatcatat ttttaattag agaaatattt tcataatttc atagccatac
     4381 tacaataata aaacgaatta ataatatgtt aattatagca ttttttattg taattacata
     4441 ttagatatgt agaatatatt ttgaaaatta aaaaaatttt ctattttata tattgaacaa
     4501 tttatgtaaa tagaaaattt ttcaataaga cttttgagtt taatcattat attggtaagg
     4561 taaacatttt taaatctaat tatatatagg tgaaataaaa aattaattat aagtaaggca
     4621 tttagaataa aattttaaaa aaattataat taaaaaaata atgaaaatat ataatattag
     4681 tttagattat aaaaaataaa tatacaaata cttataaata caagttatat acaagattta
     4741 tagtagagta ataatcttat aaaataattt aataaatata aattaaatta tatgtgtata
     4801 tattgtactt taatagtgat ataataataa aaaaattttt aaagagggtg atattacata
     4861 tgagaaaact tgaagagata ttagaatata ataaatcatt tgtagagaac aaagaatatg
     4921 aacaatatgt gacatcaaaa catccaaata aaaaaatagt agtcttatct tgtatggata
     4981 caagattaac tgagcttttg cctaaggcta tgaatttgaa aaatggagat gtgaaactaa
     5041 taaaaaatgc aggagctact ataatgcatc cttttggaag tattatgaga agtgttttag
     5101 ttgcaattta cgaatttgat gtagatgagg ttatggttgt agggcatcat ggatgtggta
     5161 tgtgtaatgt tgatacagat aagttacttg gtaaaatttt agatagagga atatctaatg
     5221 atgtaatact tacacttcgt agtgctggaa ttgatacaaa acaatggtta catggattcg
     5281 attctgcaga agaatctgta aaagatagta ttgatttaat aaaagggcat cctttaattc
     5341 cagatggtat tattgtacat ggtctaatta tatcacctga aactgggaag ttagacgttg
     5401 ttgtaaatgg atatgaagat aagtagagta ttgtaatgtt atataatcaa gatactgttg
     5461 aatatgatgt tgtttcaaaa tttattttgg gacaactttt ttatttgaaa atatatgaaa
     5521 aggtttattt taagctttac aataaaaaat ataagtagct gactaattaa aaatcttact
     5581 attatttatt aaaattataa ctatatgata taaaaatatt taataaatga aatttatatg
     5641 aattttttat aaatatattg aaaaaatgca tgcagtatag taaaataatg cttagccgac
     5701 taagtaatat aaaaaataag gagggcaagt atgaatagca attctgaaga aaaattaaat
     5761 gatgttttct tgcaattaat gcgaatatat tttataagaa ctcattttta ttttgataaa
     5821 ttaggcttat atcatggtca accccaaata cttttttctt taaagatgaa agatgggcaa
     5881 agtcaaaaag aattagcaga taaaaggaaa gtaaaagcat ctacaactac agttatgata
     5941 aagaggatgg aaaaagctgg acttgtggaa agaaaacaag atgaaaagga ccaaagagta
     6001 tctaggattt ttttgacaga caaaggttta gaaatatgta ataaagttga agaatttaac
     6061 aaggaattgg aaagagagtt ttttttagga tttagtgaag aagaaaaaat agtacttaga
     6121 agacttctaa tgcaagtacg agataattta aaagcaggat gtcataagca tgaagatgaa
     6181 aaatattttt aaagtttaaa ataatgtttt tcaatttaat gttattagac taattaatat
     6241 tacagaaggg aagaaatgta tgtttaaatt aattaaatat ttaaagaaat cggcattatc
     6301 aatagtaata atagtctgtc tattggtaat tcaagcggta tgtgaccttt ctttaccaga
     6361 atacacatcc aatatagtaa atgtaggtat acaacaaggt ggagtagaaa actctgttcc
     6421 atctgtgatt agagagagtg aattaaataa aattacttta tttatggata aaagtagtaa
     6481 ggataaagta ctagataatt atactttgct gaataaaaag gattatgtta agtataaaga
     6541 taagtaccca ggtctaaaag atgaatctct ttatgagtta aatactaaag acaaagacac
     6601 tatagatgat ttaaatgtta tttttggtaa agctatttta atagtatcag ggcttgaggg
     6661 cgatagtaaa gaggtaaaag ctatgaaagc tcagttaatg agtaaacttc caccacaagc
     6721 tactcaaagt ggagatgttg atatatttaa attgttatca gcaatgccaa aggaacagtt
     6781 agatacattg actaaagaga tgagcaaagg ctttgaaagt atgccagaga gtatgataac
     6841 acaaagctct gtttcatatg taaggtcaga atatgagaaa ataggtattg atacagaaaa
     6901 aactcaaaat aattatatat tatttactgg tgctaaaatg cttggtatag caatgattag
     6961 tatggtagct acaataacag taggattttt agcagcaaga gtagcagcaa gtgtaggtag
     7021 aaatttaaga agtggagtat ttagaaaagt tatgtcattt tcaaatactg aaatgaatga
     7081 attttctaca gcttcattaa ttactcgtag tacaaatgat attcaacaga tacaaatgtt
     7141 gatggttatg ttgcttagaa tagtattcta tgcacctata atggctatag gtggagtgat
     7201 aaaggtttta aacacaaata cttcaatggc ctggattata gcagtagcag tagttgctat
     7261 cttatcatta attgtagtgt tattctcagt ggttatgcct aagtttaaac tggttcaaaa
     7321 gcttgttgat aagttaaact tagtaagccg tgaaataatt actggtattc cagttatcag
     7381 agcttttagt actgaaaaac atgaagaaga aagatttgat aaagcaaata aaaatttaac
     7441 taaaacaaat ctttttgtaa atagagttat gacatgtatg atgcctgcta tgatgcttat
     7501 aatgaacctt atcacagttc ttatagtttg gagaggtgca tacagtgttg attctggagc
     7561 aatgcaagtt ggagatatga tggcatttat acaatataca atgcaaataa taatgtcatt
     7621 cttaatgata tcaatggtat ctattatgct tccaagagca tccgtttcag ctacacgtat
     7681 agatgaagta ttagtgtcag atataagcat aaaagaccca gaaaaatcag aggcacaaga
     7741 gtttaaggaa gataaaaagg gtcttgttga atttaaaaat gtttcgttca tgtatccaga
     7801 cgcagaagaa cctatattaa caaatatatc ttttacagca aagccaggag aaacaactgc
     7861 ctttatagga agtactggta gtggtaagtc aactcttata aatttaattc ctcgtttctt
     7921 tgatgttact gaaggagaaa tattacttgg tggagttaat ataaaaaatg tttctcaaca
     7981 tgatttaagg gaaaaaattg gatatgtacc tcaaaaggga gtattgtttt ctggtactat
     8041 agaatccaac ttaaaatatg gtagaaaaga cgcaacagat gaagaaatga gaacagcagc
     8101 tgaaatagct caagctacag aatttataga ttctaaacca gaagctttta aaactgaaat
     8161 ttctcaaggt ggaacaaatg tatctggagg tcaaaaacaa agactttcta ttgcaagagc
     8221 aatagcaaaa aatccagaag tttatatatt tgatgatagt ttttcagcac ttgacttaaa
     8281 aactgatgca gctcttcgta aggctttaaa atcaaaaact gctgaaagta cagttctaat
     8341 agttgcacaa agaattagta ctatcttaaa tgcagaacag ataattgttt taaatgaggg
     8401 agaaatagtt ggaataggaa cacataaaga gcttctaaaa aattgcgaag tttatgagca
     8461 aatagcttta tcacaacttt caaaggagga gctagctaat gagtagaatg ggaagaggtc
     8521 ctatgggaaa atctatggaa gcaggacaaa aagcaaatga ctttaaaggg actatgcgaa
     8581 aactaattgc atatttatct aaatttaaaa tatcaataat tttagtaatt gtatttgcta
     8641 ttggtagtgc atcgttttct atagtaggtc caaaaatttt aggaaaggca actacaaaga
     8701 tatttgaagg gttagtgagt aaagtttctg gaggaaacgt aggtatagat tttaatgcaa
     8761 ttggaaaaat cttaaccttt ttacttttcc tttatttgat aagtgcactg ttctctttta
     8821 tacaaggatt tataatgagt ggaatttctc aaaaagtatc ctataattta agaaaagaga
     8881 tttcagcaaa attggataga ttaccaatga agtattttga tactaagacg catggtgaaa
     8941 tattatcgag aattacgaat gatatagata ctttaaatca aagtttaaat caaagtatga
     9001 cacagcttat aacatcagta acaactatga taggtgtttt gataatgatg ttatctataa
     9061 gtggaattat gacattagtg gcagttttaa tattaccaat atcaatgttt gtaatttcaa
     9121 gaataataaa gaaatcacaa aaatacttta gatatcaaca agaatattta ggaaatgtaa
     9181 atggtcaagt tgaggaaact tatagtggcc aaacaatagt gaaagcattt aatcgtgaag
     9241 atgaagtaat tgaagagttt gataaactaa atgatagcct gtataattca gcgtggaaat
     9301 ctcaattttt atcaggtata atgcaaccac ttatgatgtt tattggaaac ttgggatatg
     9361 taatggtatc aatcttagga ggttggcttg caattaagaa aacaattgag gttggtgaca
     9421 tacagtcatt tatacaatat gtaagaaact ttacacagcc aatgactcag attgcacagg
     9481 ttgccaattt attgcaatca acagcagcag cttctgaaag agtatttgag tttttagaag
     9541 aggaagaaga agtccaaatt gttgaaaatg cagtttcaat agatggttta gaaggtaaaa
     9601 ttgattttga aaatgtcaac tttggatata acccaaacaa gactataata aatgatttct
     9661 cagtaaatgt taaacctggt caaaaagttg ctattgttgg acctacagga gcaggtaaaa
     9721 ctacaatagt taaactgtta atgagatttt atgatgttaa tagcggttca atactgattg
     9781 atggtcataa tattaaagat tttaatcgta gtgagcttag agaaatgttt ggtatggttc
     9841 ttcaagatac atggttattt agtggttcaa ttatggaaaa tataagatat ggtaagttga
     9901 atgctacaga tgaagaagtt atagaggctg ctaaatctgc acatgttcat agatttatta
     9961 agacgttacc agatggatat aagatgaaat taaatgaaga agcaagtaat gtttctcaag
    10021 gtcaaaaaca attattgact atagctcgtg ctattttagc tgatcctaaa attttaatat
    10081 tagatgaagc tacaagttct gtcgatacta gaacagaagt attgattcaa aaagctatgg
    10141 ataatcttat ggaaggtaga acaagttttg ttatagccca cagattatca actatacgtg
    10201 atgctgatat gattcttgta atgaatgaag gagatatagt tgaacaagga aatcatgagg
    10261 aacttctaaa gaaaggcgga ttctatgcca atctttacaa ttctcaattt gaagaagatg
    10321 aagcaatgta acaaaaaact atcttagcat agacatgtaa ttaataagat aaaataaatg
    10381 agggtgcctt aaaatgaact ttttaattca taaggcactc ttttgtgtat gaaataaagt
    10441 gtatttttat cattttaaca atggaaaaga ggattatcta tattttgaga tagccacata
    10501 attttatgtc tttacaaagt gtttatttta ttataatatg tagtaaggaa aatgttggag
    10561 gtgattatca aaatgtatat ttaattttat tctttaaatt ttattataaa tagaatttaa
    10621 ttttaatagt tgatgattat attgaagtat atgtttttat tacttttatt aatcatgcaa
    10681 ataactactt aaatttaaga atagggttaa atataaaatg ataattacat ataagttaat
    10741 taaactaatg tatttttaat atatatttag aattttaatc cttcttatga tataagtagc
    10801 ttgttttgta ttacttagat ttgtaaggag tttttttatt gaaaaaactg cataatatat
    10861 ttgggaggag gtgtaagaaa gtgatttttt atataagatt gagaaaataa tgcacgatta
    10921 tggttattat aatagtaaat aagaagtgtc atttaaggat aagataaaaa gcaaaattct
    10981 tgatgaattt aattatagag ccataatctc gtgctataga ttaaataaaa aatagcagat
    11041 aaggagaata tggatatgac gaaaaaagga ataacagtag gattaaacat aaataataaa
    11101 tctgaggact ttaatgaact aatgattgaa cttgaaaact tatgttcagc atgtgatatt
    11161 gatgttgtag ggagtataac tcaaaatgca aagcaggtta atagagcatt ttacattggg
    11221 acaggtaaag ttgaggaaat attaaactta ataaagaaag aaaacataga gatagttata
    11281 ttttacaatg aactttctac atcacaacta aaaaatcttg aagaaaagct taattgtgaa
    11341 attatagata gaacggcatt gatacttgat atatttgcac aaagggcaaa aactagagaa
    11401 gctaaacttc aagtagaagt agcaagttta aaatatatgt taccaagact tataggttca
    11461 aatgaaaatt taggacgtca aagtggtgga gttggtacta aaaatagagg ttctggtgag
    11521 aaaaaattag aacttgaccg tagaagaata gaagaaaaga taacatcttt aaatagggag
    11581 ttgtatgatt taaaattcca aagagaaact caaagaagta tgagaagaaa atcaaatctt
    11641 ccaaatgtag ctctagtcgg gtatacaaat gctggtaaat cttcaataat gaataaacta
    11701 gtagatatat ttaaaaactc tgaagaaaag aaagtgtttg agaaaaacat gttatttgct
    11761 acacttgaaa cttctgtcag aaatatagtt cttgcaaata acaaagaatt tttattatct
    11821 gatactgttg gttttgtaag taatctacca catgacttag taaaagcatt taggtcaaca
    11881 ttagaagaag catgtgaggc agacgtactg ttacatgtta ttgatatttc aaatccaagc
    11941 tataaaagtc atattaaagt gacagaagat actttaaaac aaataggtgc agatggtata
    12001 cctatgatac atgtgtataa taaaattgat ttaatagatg tagaagtatt ggatagaatt
    12061 ttagatagta tcgataaaga aggtatattt gtttcagtta aaaaagatat aaatatagat
    12121 aaaatgataa aatgtatttg tgatagcata tttaaagatt gtgtaagatg taagttctta
    12181 attccatatg ataaaggtca tgtagtttct tattttaacg aaaatacgag cattataaat
    12241 acagaataca gagaagatgg agctatttta gatgtagaat gtagcaatat agaatataat
    12301 aagtataaaa agtatgcttt agagtaaatt ttgaaaaact tattatagcc taaaatatat
    12361 ttaatttata atttattata aattgattgt aaatatgatt ttatgaaacg gtatagccat
    12421 aatgctatgc cgttttgcca tttataataa aaataaattt attgacacaa tagtaactaa
    12481 ttactataat ataaagtaac tagttactat ttaaaattat caaaaaatta aaatctaaga
    12541 taaagagaag tgtataataa tggatgcaga aagtttaatg aaaaaatata tggtagatga
    12601 tgatagtaga aaacaagcta tatttgcaag tatatttgta atgcaaaata aacttcaaac
    12661 tacatgtgat aaattagacc cagaattaac aatgaaacaa tggttacttt tagctattgc
    12721 ggactctgta gatgaaactc ttacattaac caaacttgga gaattaatgg ggtgctcaag
    12781 acaaaatgta aaaaaactag ctattgcttt agaaaagaaa ggttttatac aaattgagca
    12841 aaatgaaaaa gattcaagag ctatctgtct tgtgacaaaa gatagagtgc aagagtattc
    12901 taaaaaagta agaggtatgc aggaaaaagt attacagctt ttatttgaag attttacaga
    12961 gaaagaagtg gaacagttat tttttggaat tatgaaatta agtataggaa ttgaaaatgt
    13021 gaaaaattat gtagaaaata atgacgttgg agaataagca agtatcaaat caactttgta
    13081 tgacatcaga gaggggggta atttatatga aaagtgtagt agtgatttat aagagtaagt
    13141 atggttcaac aaaaaaatat gctaaatggc tttcagaatt attgtcttgt gatatttttg
    13201 aaactaagaa tattaatata gaaaaggtta tgaagtatga tacgattatt ttaggaggag
    13261 gtctttatgc ttgtaatata gcgggaattt ctttcttaaa aaaaaattat aaagatttgc
    13321 agaaaaaaaa tttagtagta tttggcgtag gagctgctcc atatgatgat gaagtaataa
    13381 agaaattaag agaagtaaac tttaaggatg aattaaacaa agttccttgc ttttattgtc
    13441 gtggggcatg ggatgagaca aaaatgtctt ttatagatta tactctttgt agttttttta
    13501 aaaaatctat agctaaaaaa gaacgtcaag gtcttaaacc agcagaagca cctttattgt
    13561 ctggagaagc ctttgattgg tgtaataaag aaaatttaac tccaatactt aattatataa
    13621 gaaaacttta agaggtatat atttataatt atttattatc taaagttaga tttcatagaa
    13681 acttactaga cattatttat aaaataatat tattaaagat ggataattta attgtttcaa
    13741 taaggttata atttt
//
LOCUS       DAEPOG010000106        13600 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEPOG010000106 DAEPOG010000000
VERSION     DAEPOG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.106"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..656)
                     /locus_tag="KR432_002477"
     CDS             complement(<1..656)
                     /locus_tag="KR432_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0641005.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="KR432_002478"
     CDS             complement(843..1352)
                     /locus_tag="KR432_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0641006.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="KR432_002479"
     CDS             complement(1427..1840)
                     /locus_tag="KR432_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0641007.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="KR432_002480"
     CDS             2277..2936
                     /locus_tag="KR432_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBH0641008.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="KR432_002481"
     CDS             complement(3543..3722)
                     /locus_tag="KR432_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0641009.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="KR432_002482"
     CDS             complement(3947..4480)
                     /locus_tag="KR432_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0641010.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="KR432_002483"
     CDS             complement(5298..5870)
                     /locus_tag="KR432_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0641011.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="KR432_002484"
     CDS             complement(5932..6195)
                     /locus_tag="KR432_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0641012.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="KR432_002485"
     CDS             complement(6195..6416)
                     /locus_tag="KR432_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0641013.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
     gene            complement(6935..7471)
                     /locus_tag="KR432_002486"
     CDS             complement(6935..7471)
                     /locus_tag="KR432_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH0641014.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(7489..8802)
                     /locus_tag="KR432_002487"
     CDS             complement(7489..8802)
                     /locus_tag="KR432_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBH0641015.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            9138..9578
                     /locus_tag="KR432_002488"
     CDS             9138..9578
                     /locus_tag="KR432_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0641016.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(9763..9951)
                     /locus_tag="KR432_002489"
     CDS             complement(9763..9951)
                     /locus_tag="KR432_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641017.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            11012..11407
                     /locus_tag="KR432_002490"
     CDS             11012..11407
                     /locus_tag="KR432_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641018.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            11450..13549
                     /locus_tag="KR432_002491"
     CDS             11450..13549
                     /locus_tag="KR432_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBH0641019.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
BASE COUNT         4914 a         2200 c         1662 g         4824 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataatt aaataagtat
     6541 ctttttttgt tcttatttta cctataaaaa ttttcctaat taatctttca taaatatcta
     6601 ttttaaataa ataatataga gtttatacaa ttttttataa cttaattact atttactata
     6661 tattattcat ttttaaataa aatgtgatta ttttttatta aaaacgatat aaatatacaa
     6721 tttaatacta tttttaacaa ataaatttat attaaaatac attaatttat atagttttat
     6781 ttattctaaa agtataatta taaaaataaa aaaagaatag gatatttttc ctactctttt
     6841 ttctgtgctt atatataata ttaaagctct ttattttcaa tatatatttt aaattttctc
     6901 tttttaatat atctttttct tttaataaat ctctttaatt ttcttcttct atatcagagc
     6961 caaagggaat ccatgcatta ccaaactctt tatctttaaa taaataagct aatccagtta
     7021 tttgcttcaa tctaagaatt tcatcaactt ccatacccag gtgctttgat atccattcat
     7081 cagattttcc aatttcacaa agttcagtta taattttact cattaaatcc acattatgag
     7141 aacctcttgc acgattgtgt cgaatagtac ttgccattct ttcatcaaga gatttatcaa
     7201 ttactgaaac aggaagaagt ccgttttctc tatcatatat atccttattt tcaagcatta
     7261 ctctatatct atgaaaacca tctacaataa tatacttatc cttttcctca ttataataac
     7321 agacaattgg cattgtatac ccatcttctt ttatactttt atacaacaac tgcatttctg
     7381 gtggggctac tgcatttgga ttatagtcgt tagattctat cttttcgatt ggcactgcga
     7441 tgattccata tacaggactt ttatattcca tagcatctta actcctatct aatgaatttt
     7501 ttcatatttc ttcttaatat attccgttct aattctctgt tcttttgtag gaccaaatcc
     7561 cataaatcta cacatatggt cattttttaa aatacaataa cacattcttt tccaacttgg
     7621 tatatctttg gtaaatttaa tatcatccgt atcatcaggt atatcatcca aaaatatgac
     7681 tctactcttt ttatcttttg tatgatttga aactccattt aattctattt tataaccttt
     7741 tctcaacaat tcacgtataa ctatttctga caaaccacca cctgtattct gccaaaactc
     7801 aatagatgtt gaaaattttt taatatagct attccttata ttttcaggta aggtatcaag
     7861 caaaaaatat gtgaaacttt tccatgtgtg tccatatggc aatttaacac tacgatatcc
     7921 catagctttt gtttttccat aaatagaagt aaaattaact ccttgtactc tagcaaccaa
     7981 tttcacccat acatctgggt ccaatattct gtataaattt aaactctctt ttgctgaatc
     8041 attaaaaggt gatgccactc tcatttttct tggagctact ccagctttat agaataagtc
     8101 ataaatttta ttatagtcat aatcaaattt atagtgggct aaccatatat cttctatttc
     8161 ccaatcatat ataggagatg ccgtccagac atttttaaat tccttagtta tccagcactt
     8221 tcctttatat ccatgctgtt tattcacaat agaacaataa cgttgtagag attctgctgc
     8281 tcgtgttcct aataagcaaa caacttttgc accttcatgt atatctctaa tccatctacc
     8341 aaattgcttt gccaaacttt cttgtctcat tttatactta taataagtaa aaggattatt
     8401 tttttgattt attatgtatt ctttgactgg catttctctt acccataatt cttctttctc
     8461 atcatcccat ggataccaat acatttcata atttcctaca gcagttcttg tagccattgg
     8521 taaacacacc caatatggtt caatatcttc aatattctct tcaaatactt tttcaacata
     8581 ttcagtagtc aaactatatt gagcctcaaa atcttgatga aatactccta ttttggaact
     8641 tatattattc tttctcttat aatccaaaac taaatttagc agtaagccac tatcttttcc
     8701 tccactaaaa gatacataga tataatcaaa ttcattaaaa agataagaaa gtctttcttg
     8761 aaaagcttca aatacatcgg attctaaata tctcttatcc atttgaatta tcccctttca
     8821 attagaagtt tataaaacct tacaatacaa tattattatg cttttttagc agtaatcagc
     8881 ataaattata cattatttaa atacacaaat ccaactaaac acatataatt ataatgtaat
     8941 tatacttttg tattataaac tatattttat cattatttta catttttttc caatatatat
     9001 aaatatcata tttttctatt agctaaaaat aattacttta tatttttaca tgttagtata
     9061 ataagacata aaaacttgac atactgttgt cataattact ttgctataat taaaagtaaa
     9121 aatggaggta aattataatg tatgaacgta tgcttgataa aagcaataaa ccctgtttgt
     9181 ctcaaataat agaatatata ggtaaagatg gatatgcacg attgtgtaag ttggaaactt
     9241 acttggaaac tcaatataat ttatcaagag aacttcgatt tccttttgga aacaattatg
     9301 gttggggtta caaatatagc cataaatctt ttcatttatt ttatgctttt tttgaaaaag
     9361 gtgcatttac tgttctgatt cagataggtg acaagcaagt tccagcagta gaaaaaacgt
     9421 tggtatcttt gacaccaaaa gctaaagaac tctgggtcac tcgttatact tgtggcaata
     9481 atggaggttg gattaagtat cgtattttag aagatgcaga cttacttgat attattcaat
     9541 atataagagc aaaaaaatct ccacctaaac aaaattaact tttatttttt gtgatttaaa
     9601 tatagtgtat ataaattcca ttctttccat attaaaaaaa taatataaaa aatatctatt
     9661 agtatatctt atttagtatg tcttattcta ctttttacag ataattaaaa agttttatca
     9721 taaataaatc ataactaaac atcctagtaa atcaaaattc atttattctt ttttattaga
     9781 atttttgata acagtcccta tacatatgcc aattatcatt ccaaaaatta gaccataaca
     9841 cattgtgata cccacatttc ctttagaaat acctatagca caacctatag ccattccaag
     9901 tatcatgcct ataaacatac atataataac taaattgttg tttttattca tagtttttct
     9961 ccattcaata tattttaaat tgcctagctc gccttcttag cttaaaacaa agaaatataa
    10021 attaactttc tttcattcta atatctgctt gtataaatgt caaacaacac atttcttgtc
    10081 ataacctcct ctttttgaag taagtacaat ctcccagaca caataatatc aatttcctaa
    10141 aaccatttaa gtaactttta tatttacata atatttacaa tcataaacta tattgttatt
    10201 catttataca tcataaacac tccctcctaa tcattattat acttaaaata atgttcattt
    10261 ataataatat atctaacgca ctttaattga aaaaactaaa actttgcctt tcattcccat
    10321 atataaataa gtatactttt tgatgctcta ttacaaatca actaaattaa ttgatataaa
    10381 ttatcatttt ctgttgaaaa taccatttac acatgctata cttaattgca taaatggtaa
    10441 taaatgtcat atgattttac aaatctatta ataacaatta ttacttttac tgtattttaa
    10501 agtttttgga ctaaatatca agaactttct aaaatacaaa gtacaaaaaa tttatataag
    10561 ttataaattt atatgaacta gataagctca aacaatatgt tatgtataaa ctgtgtagta
    10621 ttcggttata aatttatata aattatataa gctatataaa gtattacctc aactatataa
    10681 gctagatata taaattacat acataagcta gatatataaa ttgcatatat aagctatata
    10741 agaattattt caactatata aactacatat ataaattaca tacataagct agatatataa
    10801 actagatata taaactgcat atataagcta tataaaaatt atttcaacta tataaactac
    10861 acatgtaaaa ttcatgtaaa tttactaata taaatcaaat aaaattacta aactctctaa
    10921 actaattttg taaaacatac tctattttta acatttgtta ccttatacat aagtataaac
    10981 aaaatctaac taaatgaaag gataatgtaa aatgaatatt ttaaaaatga aaaaaacagg
    11041 aatattttta ggaggagtac tttttggtac tgtaggtgtt aagatacttt caagtgactg
    11101 tgtaaaaaaa acttgcactc gtgcaacagc aggagcattg aaagttaaag attctgttct
    11161 tgaaacaact acgaaaattc aagaaaatgt agaagatatt ttagctgaag caaaagaaat
    11221 caacgagcaa gaagacttat ctacatctaa ggtaaacgtt gtacaagata ctcttaaatg
    11281 cgaacaagaa gttcttaaaa ctgaaactat aaaaactgta gaagatacta attctaataa
    11341 cattaaatgt gaagaaatca aagataacgt aattgaagaa aaaaataata cttgtacaac
    11401 agaataaatt taatgattat tatgattaat aatatcagga ggaagtaaat tgaattttta
    11461 tataaaacac gaaataagag gtcgtatacg ctttgactta caacttggca aattaaacaa
    11521 caagcagtcc gatactctct tatattattt aacttctata aatggtatta ccaaagcaaa
    11581 agtttacgat agaactggtg atgcagtcat ttattacact ggtgaaagag aatacataat
    11641 aagagaaatc actaaattca gttttaacaa tagcaaaata gaaaacctag ttcctgaaca
    11701 taccagcagg gaattaaaca attattatag agacaaatta gcaaataaac tgctagtcaa
    11761 aagtatctcc aaaatatttt ttccaaagtc cttaagattt tttttaattg gacttcattc
    11821 tataaaatat tttaaaaaag gtctctcttc cctactaaat aaaaaaatag aaatttctgt
    11881 tttagatgcc actgctatag gtatttctat ttttagaaaa gatatcaata cggctggttc
    11941 agttatgttt cttcttggta tcggtgagtt attagaagaa tggactagaa aaaaatctat
    12001 agacgacttg gctcaaagta tgtcactaaa cattgaaaaa gtatggttaa aaaaagatgc
    12061 cactgaaata ctagttccaa tttcagagat aaaggaagga gatttagtca gtgtaactat
    12121 gggaaatatg attccattgg atggagttat agtatcagga gaagcaatgg tcaatcaggc
    12181 atcacttaca ggtgaatccc ttgcagttaa caaaaaagaa ggctcttaca tatatgctgg
    12241 aactggaata gagcaaggca atattgttat gtgcgtcaag gaaaaagctg gtactactag
    12301 attccagaaa atagtaacta tgattgaaga atctgaaaaa ctaaaatctt ctgtagagag
    12361 taaatttgaa catctagcag atacattagt tccatacagc tttttaggaa gtatattaac
    12421 ctatgcaatt actagaaacc ctataaaatc actctctata ttgatggtag atttctcttg
    12481 tgcattaaaa ctctctatcc ctatttcagt actatcagcc atgagagaat gtaacaatca
    12541 cagcattgct gtaaaaggtg gaaaattcct tgaaggtgtt gcatgtgcag atactattgt
    12601 atttgataag acaggcacat taacaaaagc tcaaccaact gtttcagata taattacatt
    12661 ccaaaattat aataagaagg atatgttaag acttgcagct tgcttagaag aacacttccc
    12721 acactccata gcaaatgcag tagtttatga agctgaaaaa caagggctta gtcacaaaga
    12781 aatgcataca gaggtagaat atattattgc tcatggcatt tcaacaaagg tcgatgataa
    12841 aaaggttgta ataggtagca aacattttgt atttgaagat gagaagtgta ctatcccaga
    12901 cggtgaggag gataagtaca acaatctatc agatgagtat tctcacctat ttatggcaat
    12961 atcgggtaaa ttatcagcag ttatttgcat aaatgaccct ttacgaaaag aagcaaaata
    13021 tgtaatttca aatttaagag aatatggaat aaaaaagatt gtgatgatga ctggagatag
    13081 cgaaaaaaca gctaaatcta tagcaagtaa agttggtgtt gatgaatatt attcagaagt
    13141 cttaccagaa gacaaagcca attttgttaa aagtgaaaaa ctaaaaggtc gcaaagtaat
    13201 tatgattggt gatggaatca atgattctcc tgctatttca gaatcagatg tcggaattgc
    13261 aatgagtgaa ggtgctgaaa tagcaagaga gatttcagat attaccatat cagcagataa
    13321 tttgaataat ttaatcgttt taaagcagat tagtaataag cttatgaaaa gagttgattt
    13381 aagttctaag tttattatcg gctttaattt aggtctgatt ttacttggcg taagtggatt
    13441 tgttagacca agtacatctg cctttttaca caacgcatct actgttggaa taagtttaaa
    13501 cagtatgaca aatttattgg aaaacaccaa tacttataac tactattaat ccccattata
    13561 taaaacatat gtatataaaa aatttataat tcatctttta
//
LOCUS       DAEPOG010000107        13562 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEPOG010000107 DAEPOG010000000
VERSION     DAEPOG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.107"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1169)
                     /locus_tag="KR432_002492"
     CDS             complement(<1..1169)
                     /locus_tag="KR432_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBH0641020.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTL"
     gene            1632..2828
                     /locus_tag="KR432_002493"
     CDS             1632..2828
                     /locus_tag="KR432_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH0641021.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            2921..3445
                     /locus_tag="KR432_002494"
     CDS             2921..3445
                     /locus_tag="KR432_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBH0641022.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            3470..3646
                     /gene="rpmF"
                     /locus_tag="KR432_002495"
     CDS             3470..3646
                     /gene="rpmF"
                     /locus_tag="KR432_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH0641023.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            3853..4410
                     /gene="fapR"
                     /locus_tag="KR432_002496"
     CDS             3853..4410
                     /gene="fapR"
                     /locus_tag="KR432_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBH0641024.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            4421..5443
                     /gene="plsX"
                     /locus_tag="KR432_002497"
     CDS             4421..5443
                     /gene="plsX"
                     /locus_tag="KR432_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH0641025.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            5469..6455
                     /locus_tag="KR432_002498"
     CDS             5469..6455
                     /locus_tag="KR432_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBH0641026.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            6564..7493
                     /gene="fabK"
                     /locus_tag="KR432_002499"
     CDS             6564..7493
                     /gene="fabK"
                     /locus_tag="KR432_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBH0641027.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            7509..8459
                     /gene="fabD"
                     /locus_tag="KR432_002500"
     CDS             7509..8459
                     /gene="fabD"
                     /locus_tag="KR432_002500"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH0641028.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            8459..9208
                     /gene="fabG"
                     /locus_tag="KR432_002501"
     CDS             8459..9208
                     /gene="fabG"
                     /locus_tag="KR432_002501"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBH0641029.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            9262..9486
                     /gene="acpP"
                     /locus_tag="KR432_002502"
     CDS             9262..9486
                     /gene="acpP"
                     /locus_tag="KR432_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0641030.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            9515..10753
                     /gene="fabF"
                     /locus_tag="KR432_002503"
     CDS             9515..10753
                     /gene="fabF"
                     /locus_tag="KR432_002503"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH0641031.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            10979..12697
                     /locus_tag="KR432_002504"
     CDS             10979..12697
                     /locus_tag="KR432_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0641032.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            12728..>13562
                     /locus_tag="KR432_002505"
     CDS             12728..>13562
                     /locus_tag="KR432_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0641033.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VV"
BASE COUNT         5396 a         1420 c         2575 g         4171 t
ORIGIN      
        1 atgagtgttt taaaaatcgt atcatctaat gaaaatgata tatttgaaaa cttgtttatt
       61 atttttattt cagatttatt ttttatctct tttagaattt ctctaccttt atcattaaaa
      121 gcaagtattc ttatataagg tatagtatct atgtttttta taagttgcat gtcgcttttt
      181 ttaataccta acaatatatt gtttagagtt cgtttgattt tggttaaagt gtatcgcttg
      241 gatttaattg ctaattgtag ctcatgtaga aattcagatg taaagatatc tttatagatt
      301 ctgttttcta tgccttcatt tacttcaaaa tactctttta aagtgtccat atctctaagt
      361 ataattgttt ttaaaatatc aaagaagaag tcatcaaaca taggtaaaaa accattatct
      421 actttttcat ttaggatgtt ataagttttt tctggaataa cattttttaa atgaaaaagg
      481 tctgtatttt ctttaagtga agtccttata gcagtagcag aacaaatatc actctcaatt
      541 tcttcggaat tatattctga ttggaccctg gagatagtaa atggttttat actactgttt
      601 aaatggatga ggttttttat gtattctata cccagtatgt tgtttgatga atttaatgtt
      661 tttgataatt gttcctttga ataattttgt ataaaattat tttttttgtc ttgatgtata
      721 taatcaaaaa gtgcattagc tctagctgtt ggaaataaca ggccatcatc taaatatttt
      781 tttaaaagta acttaaattc atttggttca tttgctaaaa tttcagctat attataaaga
      841 atttctgtat ttccttcttc actaccaaaa catatagaat ttatgcagtt tagtgaattt
      901 aaaattgtta tagcaccatg agaaaaaatt tcagcacttt gacaagcaaa taaagttggt
      961 aattctataa ctaagtctac accattttcg acagcaattt ttgctctagt atatttatca
     1021 aataaagctg gttcgcctct ttgtagaaaa tttccactca ttattgctac agtatgtgtt
     1081 gcatttgttt tttctataga tttagataaa tgatatatgt gtccattatg aaatgggtta
     1141 tattcaacta ttaatccaag tatgttcata aaatactcct tttatcttta attattttat
     1201 atatatcaca atttattata atatgtataa aatttaaatt tttaattata aaattaaaaa
     1261 acgtaaaaaa atttataaaa atgtagaaat gttacctaca taaaaaactg gttaaatgtt
     1321 attaaaataa ctttattagc tctttatatt atattattat aacaaatggg aaagaataat
     1381 ctacattaag ttaacttgtt aatatattat tagtgaaata cacaatttac catactagaa
     1441 atatgatata atataaaaaa aaagtatata cttttatatt aaaaggtgat atattattta
     1501 tgaaaatcgt ttaatgtata caataatata tctataacaa aataataaaa gatagagttt
     1561 tatttagaca tttattacaa atcaaaattt attacatatt atttataaaa aaatctaagg
     1621 aggaaataat aatgaaaata ttagtattaa actgtgggag ttcttcatta aaatatcaat
     1681 taatagatat gaataatgaa gaagttttat gtataggatt ggttgaaaga ataggaatag
     1741 aaggttctat attaaaacat gaaaaagcag gtagagatga taaatacgtt gttgaacaac
     1801 caatgaagga ccacaaagat gcaatagcat tagttttaga agctgttgct catccagaat
     1861 ttggtgcagt taaagaaatg aaagaaatag atgcagtagg acatagagtt gtacatgctg
     1921 gagaaaaatt tgcaacttca gtagtaataa ctccagaagt agaagaagct ttaaaagaat
     1981 gtatagattt agctccactt cataatccag caaacataat gggaatagat gcttgtaaag
     2041 ctatacttcc agatgtacca atggtaggtg tatttgatac tgctttccat caaacaatgc
     2101 ctaaatcttc atacttatat ggtttacctc atgagctata tactaaatat ggggtaagaa
     2161 gatatggatt ccacggaact tctcataatt atgtatctca gagagcagca gagatattag
     2221 gaaaagatat aaaagattta aaaatagtaa cttgtcactt aggaaatggt gcttctatag
     2281 ctgctgttga tggtggaaaa tgtgtagata catctatggg attcactcca ttagaaggat
     2341 taataatggg aactagatgt ggagatatag acccagctat attaccattc ttaatgagaa
     2401 aagaaggttt agatgctgat ggattggata aattaatgaa taaagaatct ggagtatatg
     2461 gaatgactgg aatttctagt gacttcagag atatagaaga tgcagctaaa aatggagacg
     2521 aaagagctca ggctacatta gaagcgtatg ttaaaaaagt acaaaaatat ataggcgctt
     2581 atgctgctga aatgaatgga ttggatgttg tagtatttac tgcaggcgtt ggtgaaaatg
     2641 gaaaggctat aagagcagat attgcttcta acatggagtt tttaggaatg aaattagata
     2701 aagaagctaa tgatgttaga ggaaaagaaa cagtaatatc tactgctgat tctaaagtaa
     2761 aaatgctttt aataccaact aatgaagaat taatgatagc tagagataca ttaagattgg
     2821 taaaataatt aaattaaacc tatgcctatc aagtaaaaat acttgacaaa caaatgctag
     2881 atttgtataa taaatttggt gatatgttga ggtgaactta atgaaagtta gcatagaaaa
     2941 aataaataga aaagaaactg acaaaataga cttgaatttt tgtgaaaaaa ttgatactat
     3001 tagttattgt gatgagattt acaaattagt atcacctgta aatttaaaag ggaaagtgtc
     3061 aaagactaat aaaggtttgt atctagatat agatgtcaat tttacaattg ttgacacttg
     3121 ttcaagatgt cttaaagaag tagaaatacc actagagtat tctatacaag gttttttagt
     3181 aaaagaagaa gattatgatg aagatgagtt tgaggaattt gatcctttta tattcgatgg
     3241 tgaagaaatc gaccttattg atataataga acagacatta gattttaatg tacctcataa
     3301 agttctttgt agtgaaaact gtaaaggtct ttgtcaagta tgcggggcaa acttaaatga
     3361 agaagagtgt tcttgcagtg aaattacaaa cgatgaggaa tacatagatc ctcgttttgc
     3421 taaattaaaa gatttattta attaaatttt gactaaggag gtggcgtaaa tggcagtacc
     3481 aaagcgtaaa acgtctaaat caaacacaaa aatgagaaga gcagctaact caaagatgga
     3541 agcaacagga tttgtaagtt gcccacaatg tcatgagcca aaattaccac atagagtgtg
     3601 tccagactgt ggatattata aaggtaaaga agttgtgtct aagtaatagc ttctaaaaag
     3661 catgtagagt atactctaca tgtttttttt gtacatataa aaaaataaga tgatttacta
     3721 gagaaaataa gtggatttta tgcatattaa aaaatagaaa aataataata taagtattga
     3781 aaatttttgt gtgttactat atactactat tagtagctgg tactaattac agctataaaa
     3841 tttggaggga acatgaaaaa gaaaagtaaa gcccaaagac aaaaagagct tatagatatg
     3901 ctaaagacag atccttttta tactgatgaa gaattatcaa gtctgtttga tgtaagtata
     3961 cagaccataa gattagatag aatgagtcta aacataccag agctaagaga aagagtcaaa
     4021 tcaatagcag aaactcagag ttcaaaagta aagactttag gagtaaagga aattacagga
     4081 gaaatcattg atttatctgt agggcgatta ggtatatcta tgttagaagt tacacaggac
     4141 atgatttact ctaagactaa tactttaaaa gatacgtata ttttttctct agcagattca
     4201 ttggcaatgg ctataataga tgcacctaag gttattatga gagttgcaaa tgtaaaaagt
     4261 tttaagttga tagaacaaca agatagactt atagcaaaag cagaagtgta tagaaatata
     4321 gataaaaagc attatgtaaa agttgttatc aacaataaag cccaagagca aatatttaga
     4381 ggaaagttta tctttgaaga attagattag gagggaaatt atgaaaatag taattgatgg
     4441 aatgggtgga gataatgcac ctaaatctaa tgtagaaggc gcagtaaatg ctataaaaga
     4501 atatcaggtt gatttaataa tcacaggaga taaagattta cttgaaaagg aattttcaaa
     4561 ttatgaattt gatagaaata aattagagat agttcatact actgagatta tagaaaacga
     4621 agataaacca gtaaaggcta taagaagtaa aaaagattca tctatggtag tagcattaaa
     4681 tcttgtaaaa gaaggaaagg cagatgctat aatatcagct ggaaatacag gagccttact
     4741 tgctggtgga ttatttgttg ttggtagaat aaaaggaatc gatagacctt gcttatgttc
     4801 agcaatacca aatgtaaagc gaggaatgac acttatagca gactgtggcg caaatgcaga
     4861 ttgtaaacct aagaatctag ttgaatttgc agctatgagt aatatttatt caagaaaagt
     4921 tttaggtctt gaaaatccta aggtagcatt agcaaatgtt ggactagaag aaggcaaagg
     4981 caatgattta gtcaaaaggt catatgaaga aattaaaaaa ttagatttaa attttatagg
     5041 gaatgtagaa gctagagaag ttataaatgc atacacagat ataatcatat gtgatggatt
     5101 tacaggtaat atattactta aatctgctga aggtgtagca ctttcagtta tgagtcttat
     5161 aaaggaaact tttatggcca gtacaaaaag taaaatagga gcattactta taaaggatga
     5221 tttaagaaaa ttaaaaagtt ttatagatta ttctgagtat gggggagctc cacttttagg
     5281 tctaaatgga ggagttataa aagctcatgg tagttctgat gctaaagcta taaaaaatgc
     5341 tataaatcaa ggaataaaat tttcaaaggg aaaagtcgtt gaagatatta accaatttat
     5401 aagcaaatac aatgaagaaa ataaaaataa cgaagatgaa taaactcgtt ttggaggttt
     5461 aatatataat gaatacaaag gctggaatat taggagtagg aagttatcta cctgagcaat
     5521 catatgataa ttttcacttc gaaaaaataa tggatacatc agatgagtgg ataagtacta
     5581 gaactggtat taaggaaaga agatttgcca aagagtctga agcaacttct gacttagcat
     5641 ctaaagctgc cttaaaagct atagaatgtg caaagttaaa cgtagaggat atagagctga
     5701 ttatattagc tacaattact cctgatatgt ctttgccttc aactgcatgt atagtacaag
     5761 atgcaatagg agcagtgaat gctactgcat ttgatatatc agcagcttgt tctggatttg
     5821 tatatggagt aactattgca aagcaatttg tggaaacagg ttgctataaa aatgtgcttg
     5881 taataggagc agaaacttgt tctaagtttt taaattatga tgatagaaca acagcagtat
     5941 tgtttggaga tggagctgga gcagctgtta ttggtcctgt aaatgagggt ggaatactgt
     6001 ctacacatat gggctctgat ggtaaaggaa aagattgttt aaaagtacct gctggaggtt
     6061 caagacttaa ggcaagtaaa gagactgtag aagctaatct acatacaata gaaatggcag
     6121 gtagtgatgt gtttaaattt gcagttagaa agatggcaga aacttcttta agagctcttg
     6181 aaaaagcaaa cctaaataca actgatatag actacttagt gccacatcaa gctaatataa
     6241 gaatcataca agcttcttct aagagattag agcttgatat gaaaaaagtt tatgtaaata
     6301 tagacaaata tggaaatatg tctgctgcat ctataccagt agcattagat gaagcttata
     6361 gagaaggtaa aattaaaaag ggtgataatg tagttttagt tggctttggt ggaggtctaa
     6421 cttggggggc ttcagttgtc aagtggactt tatagtataa aatatctaaa atgatttaat
     6481 taacaaaatt aaacttatac acaaaataaa gttgattaat aataaaaagt ttacttttat
     6541 gatttcaagt gaaggagaat tgtatgaata aaatttgcaa aatattaaat ataaaatacc
     6601 cagttatcca agggggaatg gcatgggtag ctactgcatc attagcaagt gctgtatcta
     6661 atgcaggagg acttggcata atagcagcag gaaacgcacc aaaagaagct ataaagaaag
     6721 aaattgttga gtgtaaaaaa ttaacagata aaccttttgg agtaaatgta atgcttatgt
     6781 cgccatttgt tgatgatata attgatttga ttatagaaga aaaagttcaa gttattacta
     6841 ctggtgctgg aaatcctgca aaatatatgg atagattaaa ggaagctgga acaaaggtta
     6901 ttcctgtagt acctacaata gctttggcac aaagaatgga aaagttagga gctacagcag
     6961 taatagcaga aggtactgaa ggtggaggac atataggaga acttactact atggtcttag
     7021 ttccacaagt tgctgatgct gtaaacatac ctgtaatagc tgctggagga attgtagatg
     7081 gtagaggaat tgcagcatca tttgcattag gtgccagtgc agttcaagta ggaactagat
     7141 ttatttgcag tgaagagtgt tctgtgcatt caaactataa aaacttagta ctaaaagcaa
     7201 aagatagaga tgcaattgta acaggaagaa gtactggtca tccagtaaga acattaaaaa
     7261 ataaactatc aaaagaattt ttaaagatgg aacaaaatgg agctactcct gaagaattgg
     7321 ataaaaaagg tacaggagct ttaagatttg caacagtaga tggagacata gaaaaaggtt
     7381 catttatggc aggtcaaagt gctgctatgg taaaagaaat aacaccttgt aaggaaatta
     7441 tagaggctat ggtaaatcaa gcaagagaga ttatgccagc aatagaactg taaatcagga
     7501 ggagcaagat gggaaaagtt gcactagtat tccctggtca aggagctcaa tatgtaggta
     7561 tggccaagga tttatatgaa aataatgctg ttgctaaaag tgtaatagat gaggctagtg
     7621 atgccttaaa tatggattta aaaaacctta tgtttaatgg aaatgaagaa gaactttcta
     7681 aaactgaaaa cactcaacct gctatggtta cacatagtgt agcagtatta aaagcggtac
     7741 aagctcaaat agatttaaaa tataatgcat gcttaggatt atccttagga gagtatagtg
     7801 cgttggttgc agcagatgca attgacttta aagatgctgt atgtttagtt aaaaaaagag
     7861 gtaagtttat gcaggaaaca gttccagcag gtgttggagc tatggcagca attttaggtt
     7921 tagatagaaa tgtacttgaa aatgtggtta aagatataca aggaggtatt gtagaagtag
     7981 caaattacaa ttctcctgga caaatagtta tatctggtga aaatgaaaag atagaagaag
     8041 caatggtaaa atgcaaagaa gcaggagcaa aaagggcagt taaattaaat gtaagtggac
     8101 cttttcactc atcaatgtta aaagaagctg gtgtaaaatt ggccagtgaa ttagaaaaag
     8161 ttaacataac taaacctaaa gttgatgtgg tggcaaatgt aaatgcagat tactataaaa
     8221 acgaaggtaa agacctgctt ataaaacaag taagttcatc tgttctttgg gaagattcta
     8281 tagaaatact tttaaatgat ggatatgata cttttataga aatgggacct ggaaaaactt
     8341 taaaagcatt tattaagaag atagcttcca ataaaaaagc aaatgtcaat atttataata
     8401 tagatggtat agattctctt aatgaatttg tacaaaatta tagaaatgga gagatataat
     8461 gataaatctt acaggacaag tagcagttgt tactggtggc tctaggggta taggaaaaga
     8521 aatagcaaaa aaactagcat cttttggagc tgatgtagta atcaattata cttctaaaga
     8581 agatgaagca ctaaaaacta aaaatgaaat agaaagtatg ggggtaaagt gtacctctat
     8641 aaaatgtgat gtgtctaaat ttgatgaagt gaatcaaatg atagattctg ttgtaagcga
     8701 atttggaaaa attgatatat tggttaataa tgcaggcata actaaagatg gtctgcttat
     8761 gagaatgaaa gaagaagatt ttgatagagt tatagatata aacttaaaag gtgtctttaa
     8821 ttgtacaaaa gcagttacta aacctatgat gaaaaagaag tatggaagaa taataaatat
     8881 gacttcagta gttggaatta tgggtaatgc agggcaaact aattattgtg catcaaaagc
     8941 aggtgtaatt ggatttacaa aagcttctgc aagagagtta gcatcaagaa acataaatat
     9001 aaatgcagta gcacctggat ttatagaaac agatatgact agagtactaa gtgatgatgt
     9061 aaaagaatca acactagcaa acataccaaa gaaatcttat ggtaaaccag aagatgtagc
     9121 caatgccgta gcatttttag ttagtgacat gtcaagttat ataacaggac aagtaataaa
     9181 tgtagatggt ggaatggtaa tgcaataata atttaaaaag tagagaaatc tattaaaata
     9241 aaaaatttta ggaggtacac aatgtttaat aaagttgtag aaataataat agagcaatta
     9301 ggagtagaag ataaagaaat aactatggag acatcattaa tgaaggattt agaagcagat
     9361 tcattagatg cagtagaaat aataatggca cttgaagatg aatttggtat agaaatacca
     9421 gatacagaag cagaaaactt caaatctata ggagatatag ttaagtatat agaagctaat
     9481 aaatagtcga gctttaaaaa agtggaggta tccgatgaat aaaagagtag taataacagg
     9541 acttggatgt gttacgccgt tgggaacggg aaaagaagag ttctggagta atataaaatc
     9601 tggagtatct ggtatagata aaataactaa ttttgatgct agcacatatc aaactcagat
     9661 agcaggagaa gttaagaatt ttcatccaga agaatatata agtaaaaaag aattgaagag
     9721 attagataaa tttgctcaat ttgcaatagt atctgctaaa ttagcagttg aagatgcaaa
     9781 tcttgattta gataaagttg atagagaacg ttttggtgtt ataataggtt ctggaatagg
     9841 tggagttgaa gctatagaaa ctcaacataa aattcttttg gaaaaaggaa ataaaagagt
     9901 aagttcactt tttgtaccta tgatgatagg aaatatggca gctggtcaag tttcaatatt
     9961 tttaggagca aaagggccta acactaatgt atgtacagct tgtgcatcag gaactcattc
    10021 aataggtgat gcttttaagg taattcaaag aggtgatgca gacatcatgg tagcaggggg
    10081 ctctgaagct gctgtaacag gtcttgcttt tgctggattc tgtaacatga aggctatgtc
    10141 aactagaaat gatgacccta aaactgcatc aagaccgttt gataaagaca gagatggttt
    10201 tgtaatggga gaaggagcag gtattgttat cttagaagac ttagaacatg ctttagcaag
    10261 aggggctaaa atatatgcag aagtagttgg atatggttta actgctgatg cataccatat
    10321 gacaactcca gctgaaaatg gagagggagc agctaggtct atgaatatgg ctttgaaaga
    10381 tggaaatgtt ccattagaag aagtagatta tataaatgct catggaactt caacttatta
    10441 caatgattta tatgaaacta tggctataaa aactgtattt ggagaaaagg catatgactt
    10501 atgtgtgtca tcaacaaaat ctatgactgg tcacttatta ggagcatcag gtgctataga
    10561 ggctgttgtt tgtgctatga gtatagaaga tagttttgtg cctccaacta taaacataca
    10621 agaagttggt gaagatttgg atttagatta tgttccaaat caaggtaaag aaaaaaatat
    10681 tagatatgca ctatcaaatt ctttaggatt tggtgggcat aatgctacaa tagtactaaa
    10741 aaaatatgta taaataaata ttaaatataa aactaaaaaa caagcacttg ttttaactgt
    10801 ataaatttat attagcttaa atttaaagtc tcctaaatta ttttaggaga cttttttatt
    10861 aagatttaag tctagcatat gttgtaaatc attttgatat attgtattat taattattaa
    10921 gaggttattt atatatgcaa gttttcattc tacaatatac aaaaggagga ctaaaatttt
    10981 gaattttaga ctaccaaaga aaaggttacc caaaaagata atttttatat gtataatact
    11041 tattagtatt attttgttta taaagtatga ccaaaatgga ttgaaagaaa gagaaattat
    11101 aaataaggga aaatatatgt ctgaaagcct tgtggcagat aaggagtatg atgaagcaaa
    11161 aaaagtggtt aagacttctt tcgaatctat tccagaaaaa aaatacaata aagtatataa
    11221 tgaagtttgg gatatgttaa agactatttc atatgttcca gatggaacga agatagtcat
    11281 tgattcttta gaaaagatga gagaatcaga tgtgaaatta tctgatgaat gtagatttaa
    11341 tatagaaaaa agattagcat cagtgtatat aatggataat aattattcta aggcagtaga
    11401 tttaactgta aagtctataa aattggctga gaaattgggg aataattatg aaaaagcaag
    11461 actagatgtt gatttggcga gcatattttt aaatataggt agctatgaaa caggagagaa
    11521 attaataaag gattctttta aaatagatat tgatgaaggt gaaaaaaatg gaatggttag
    11581 attatatgct attataaatc tagcagaaat atatgtagaa gagggagaat atgataaagc
    11641 tattgaaata agtagcagga taaaagatta taaaaagttt gtaaatacgg ataattacaa
    11701 tgattttgat ataatggcct ctataatgaa gtcaaatgcg tatgtgggca aaaataatat
    11761 acaaaaggca aagacttttt tagaaaaagc tgattatctt ataaaatcag atagaacagt
    11821 ttatatatta gataaagata tgtactatta tatggcaatg ggaaacttag aatataaaag
    11881 cgaaaattat aattctgcaa tagataatta taaaaaaagt ttagagatat ctaccaaaag
    11941 aaagttaact caggagaaaa taaaaaattt aaataagata ttaaatatat atgaaaaaca
    12001 aggaaatata gaatctttaa atgagtatca aaagatgtta ataaatgaat ataaagagaa
    12061 caaaagtata agagattcag agactagttt ctatataatt gataaagtat caaacgaaag
    12121 taagctattt gaaaaaacta aaaaagaaat aaaatattat aaaatattat ttacagtaat
    12181 tttaattgct atattagggt ctactttttt atacaaccga ttgaagtatt ttaaagcaca
    12241 aaatttacat gatggtctta caaatgttta taatagaaaa agttttgata ttatgtatga
    12301 aaagtacaga gagaaagatg ataattttgc acttgtgatg atagatattg ataatttcaa
    12361 gctcataaat gataattatg gacataaatt tggtgacact gttataaagg gaataacaaa
    12421 taccatttgt gttatgttag aacaagatga taaagtcttt aggtacggag gagaagaatt
    12481 ttctgtatta ataagaaata aatctggtga ggaagtagca gatataattg acaatataag
    12541 ggttgcaatt gcaaataaaa agtggaatga tgatgtgact gtgactataa gtgcaggtgt
    12601 agctcatatt tcagatagca aagatgtact agaagaagct gataaaaatt tatataaagc
    12661 aaaacaactt ggtagaaata aagtagtata taagtaatta tatactatat aaataagagg
    12721 tataaatatg aaacaatcaa tagctatagt aactgataaa ataactaaac tatcatcttt
    12781 cttagaagaa aatatacgtt tggttttagg taattatatt gatataaacc actattatct
    12841 ggaagtttta aaagagaatg ataaaataca aggtgatttt gtactagtaa tgaatgatga
    12901 tagattaaac agtatgagaa agcatttagt acaagatagt aagattatag tgataaggag
    12961 aacacttaaa gaaaatgaaa tatatggatt attttcaata ccaaatggaa ctgaagtatt
    13021 ggttgtaaat gatacaaaag aaactacctt agaaacgata agcttatttt acaaaatagg
    13081 agtaaaaaat ttaaagttta ctccatatat tgaaggaaaa atttataaaa atatagacat
    13141 agctataacg cctggagtaa aggaaaaagt accaagttat ataaataaag taatcgacct
    13201 tggtaataga tatattgata tatctacttt tatagaaata ataaatggtc ttagaattga
    13261 tgttaaagaa atacgtaaaa atttaatggc atattctgag agtttaataa gtttagataa
    13321 tggagttaaa gataattata ggcaactgtt tttaaaaata gaagaacaag atgttatatt
    13381 gaatctatca aaagatggaa taatatttac ttcagcagat ggtataataa acacatttaa
    13441 taaagaagct ttaaaaattt taggtataaa tgaaaatcca caaggcaaaa acatagaaga
    13501 tatcatttct gaagatttaa atattttact tgaagacaaa gaaataattg atgaagtggt
    13561 at
//
LOCUS       DAEPOG010000108        13505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEPOG010000108 DAEPOG010000000
VERSION     DAEPOG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.108"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(225..1892)
                     /locus_tag="KR432_002506"
     CDS             complement(225..1892)
                     /locus_tag="KR432_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBH0641034.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1966..2721)
                     /locus_tag="KR432_002507"
     CDS             complement(1966..2721)
                     /locus_tag="KR432_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBH0641035.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="KR432_002508"
     CDS             complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="KR432_002508"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0641036.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="KR432_002509"
     CDS             complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="KR432_002509"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0641037.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4069..5379)
                     /locus_tag="KR432_002510"
     CDS             complement(4069..5379)
                     /locus_tag="KR432_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBH0641038.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5458..6174)
                     /locus_tag="KR432_002511"
     CDS             complement(5458..6174)
                     /locus_tag="KR432_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBH0641039.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6419..7690
                     /gene="sleC"
                     /locus_tag="KR432_002512"
     CDS             6419..7690
                     /gene="sleC"
                     /locus_tag="KR432_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBH0641040.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8160..8816)
                     /locus_tag="KR432_002513"
     CDS             complement(8160..8816)
                     /locus_tag="KR432_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641041.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(9929..10339)
                     /locus_tag="KR432_002514"
     CDS             complement(9929..10339)
                     /locus_tag="KR432_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641042.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10555..11937)
                     /locus_tag="KR432_002515"
     CDS             complement(10555..11937)
                     /locus_tag="KR432_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0641043.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(11948..12307)
                     /locus_tag="KR432_002516"
     CDS             complement(11948..12307)
                     /locus_tag="KR432_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641044.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12512..13291)
                     /locus_tag="KR432_002517"
     CDS             complement(12512..13291)
                     /locus_tag="KR432_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBH0641045.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4589 a         2425 c         1346 g         5145 t
ORIGIN      
        1 aaaattatca cataaactaa caaaatatta aacaaaaaag taaaatttaa aataaaaagc
       61 ttgcttcata caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct
      121 gtatcaattt aattctccac tttacactta atatacttaa cataaaccaa tcacactact
      181 tgctatgcca tttaatcttc ataaaacaat ctataataca catattaatc aactatttta
      241 cttcctccaa taaattccct aagaattgaa atacctggta tgtcagaagt atcgtccaat
      301 tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt caatatgtgc aggattatct
      361 tcctttatct cttccaacaa aggtttcgca aatcgtttta aaactgctaa ttcatataca
      421 caagcagagt taaatatagc atctgcctcc tcttggtacg ggaatatatt cttcttttct
      481 cctcttctaa ctgatgccca ttgtaaaata gtattatcag cactatatcc tctaaaatta
      541 tagtctctaa caattcttct tatcattctc aaatcagcag tagggattcg attatggtca
      601 tccaaattta tttgagtaag ggcacttatg tatatcttaa atttatcttc atctggtata
      661 gatgatgtta aaataggatt tagcccatgt ataccttcta atataattgg ctgatttgaa
      721 tctatcttta tttttttacc agtgtattct ctcttacctt ctttaaaatt gaatcttata
      781 tcatcaattt cttcaccagc taaaagtttc tttaagtcta agttaaactt ctctaaatct
      841 attgcataaa ttgattcaaa atcataattt ccatattcat ctaaaggagt atgttttcta
      901 tctataaaat aatcatctaa agaaattgaa ataggattta aattatttac tctaagatgt
      961 attgataatc tgtgagcaaa acttgttttt ccagatgaag atggagctgc tattaaaatt
     1021 actctcttat tgccatcctt tatcatatcc gctatttggg ataacttctt ttcatgcaat
     1081 gcttcgaatg tccttattat atctccatat tcattgtttt ctattatttt gttaagagat
     1141 actaccttgt caattcccat aagtcttgac cattcttctg cttcataata tacacttgat
     1201 agcttaggtt gaggtacaaa cttcatagga agagtcttat cttcttcact aggccctaaa
     1261 attataacgc ctccattata aagcttaata tcaaatgatt ttatataacc agttgatggc
     1321 aacatataac cataaaaatg gtctatatag tttctacact tatatacttt agcactatca
     1381 tactctttgt atttcaaaag ctctgccttt tcatacattt cattattttc aaatatagaa
     1441 atagcttcat ttcttgtaac ttcaattttt tctataacat aatcgttgtt tactatctct
     1501 ttcatcttgt tttttatatt ttcaacatct gcttctttaa gctttctatc tatatgaact
     1561 tcacaatata gcccatctga tagtgaatgt tctattgtaa ctctgctatc ccaaaagatt
     1621 tctctacaag ccattacaaa tataaatgac atgactctaa agtaaactct ctctccatct
     1681 tcattagtag tgtctaaaaa atttatctca caatccttct taactctaca atttagctct
     1741 tttagtttat tgtctacaac tgcaagtgtt atataaccct tatgctcatc acaaaattct
     1801 ttcgctatat ctcccaatct gattccaggg gaattttctt ctaatgaata ttccttatct
     1861 tttccttcta tttttaagtt tatttttttc acttcaacat ctccttcctt ttgatgaata
     1921 taaattttag ttaatgaggc atatcaacaa gataaagaga ggtatttaac cctctcttac
     1981 aatattattc aatttactta tagtatcttc aatgtcttcg taatttattt ctaatactaa
     2041 atattctgca tctaatgtta agacctcttt aatcccctct tcagataaaa ttcctcttcc
     2101 tataccaaca tgtgtatcat tgataccatc attatcactt aagtgtatca gtcttatttt
     2161 atcctttaag ttctttacat aagcatcctc atcaattaaa cagtgtccag tatcgtaaca
     2221 aaaacaaatc ttagaatttt taatcctctt aaatatatat tcaaaatcat aagatatatt
     2281 tccaatattt gaaaaatccc caccttttga ataagtattc tctatacata catatgaatt
     2341 tgtatttaat ttatctaaaa aatccacact atttcctaaa tatttgtctc tattctttct
     2401 aactctattt gtcataacat atcccaaatg aaggttaaaa taccttatat caaatccttt
     2461 tagttcaaat tctatcttct caataaaatt tatccatgaa tttcgtatat actctatgtt
     2521 ttcgcaagta tttaattcca taggcaagtg tatccctata gataatccta acttagaaat
     2581 tctctcttta tatttacata actcgctaca ttcagatata ttgtcaattc caatctctaa
     2641 atgagttatt tgctttatac tctcacaaat gtttaaagcc tcctcaatat taaacaataa
     2701 ggacgctata ccaactgcca tacaaatcac ctactcaaaa atacctatat ctgtaaaagg
     2761 aaatactctt aaaataactt ttcctttaat attttcttca tctaccaatc ccacttcttt
     2821 atatctactg tctaaactaa cttctctatt gtctcccatc gcaaatactt ttcccttagg
     2881 aatcaccata tcaatatcgc cttcagtata attatcatgt atatatgaaa cttcatttaa
     2941 taacttacca tttatataaa ccttagaatc cttaattttt agatggtcac caggaacacc
     3001 tataaccctt tttacaagct cttttttcct tccattctct tgtaataaat cagtcttaaa
     3061 tatcattata tctccacatt ttggttctga taacttatat gtcatcctat ttgctaccaa
     3121 ataatcatgt gatttaagtg tcgggtacat tgattctcca tttactagtg ttggttttat
     3181 aaaacgagtt ataataaatg caaaaaaaag tgcagtaata attattttta tccactctac
     3241 aatctcttct ttaatagttt cattcattca caaattctcc tttatagtta tattatcttt
     3301 attgataatg tctcttaaac actatctttt agataagtct actaaataag gtgttgtctt
     3361 ttttatatag acaactattg tgtatggtct ggtgatttca gctaatcagc gattgctcca
     3421 aattttatat gtttagtcaa tagttcctat cttagaaaat ggatacaatc tgactagaac
     3481 ctttcctaaa atggtatgct cgtcgaccaa tccaacctca tcgtatctac tatctaaact
     3541 tttttctcta ttatctccca ttgcaaataa ttttccttct ggaactacga tatcaatatc
     3601 tccatcagta cgattattat gtatatagga agtctcatct aacaacttat catttacata
     3661 taccttggag tcttgtattt ttacatggtc accaggaacc ccgataactc tttttactaa
     3721 atcttttttc tttccatttt cctctgttaa gtcggtttta aatactatta tatctttgta
     3781 ttttggctct cctaccttgt acgcaattct gttaactatc aaataatcac gttcaactaa
     3841 tgtaggatac attgattctc ctttgactat tgttggcact ataaaacgag ttattgcaaa
     3901 tgccaaaaca agagctatga caatcacttt tatccattct acaacttctt ttttaactgc
     3961 ttcacccact aaaatcgtct cctctgtgtt aaatattaag tcaatctaca gcaaaataat
     4021 ctaccataaa cctacaatat aaatattata cttctttttt ctaatagttt aaaatacttt
     4081 tctaagacct ttaggataat gattcttaat tacaccagaa gattcttttc cccaacctaa
     4141 aggtattcct tcaaccgaaa ccaaaaccca tcctctacta tcattagaat tcacaacatc
     4201 cccttttaaa tatttaagaa ttgattcact ctgtacactg aagttttcaa cattttttac
     4261 atcttcaact tttaagtaat gagatagtgc atgagaaggc tcaaatctat tcttctttaa
     4321 aacccctaaa tgcaaaccgt atctcaatac ttttagtttt ttcgtatctg gatatatttc
     4381 aggaagtagg tacaaatttt ctcctcttaa ataaaattta ttttttttat tactataaaa
     4441 atcaaccttt aaaaatttct tttcaaactc cctataatcc tttatttctt tatctaaatt
     4501 ttttattttt aattctttga cattacattc ttcttcttct aattttttta ttttggcaac
     4561 aaaatgacct tctcccttta tcttatgagg ccataatctt tccatttcaa taagttctgc
     4621 atgtggatat tcactgataa actcatttat aacatgctca ttttcctccc ttgaaaaagt
     4681 acaagttgag taaattaaaa tgccatcctt ctttaacatt ttatatccat ctctgataat
     4741 ctctttttgg atggattgac attctactac ctttgcataa ctccaatctg atatggcaac
     4801 ctcatccttc ctaaacatac cttgcccaga acaaggtgca tctataataa ccttatcaaa
     4861 atacccatta aacgtctttc ttaagctatt agaatcggtg tttgttataa tacaatttct
     4921 cgctccaaat cgctctaaat tttcccctag agccttaatt ctactagcat taatttcatt
     4981 agaaaccaat aaacctgtat cgtttagctt agatagtata tatgtagatt ttccaccagg
     5041 agcagcacac atatcgagaa ctttttcacc ctctttaata tctgcctttg gtattacact
     5101 cattgcagaa ggctcttgca aatagtatgc tccagcctca tgcagtggat ttctaccagg
     5161 tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc catggtattt gattcaaatc
     5221 aaacaactcc aaatcaaata attttttctt gtcaattttt aaagtattta ttctaagccc
     5281 agtagtctta ctttcctcat aactttttat aaaatcatca tattcatctt ttagtatttc
     5341 ttcaatatct tctaaaaatt cctttggtaa ttctgtcaaa tctataatcc cccttcaatc
     5401 tgctttactt gtgtgtcatt tattatactt taattattta tgtctttcct ttaaatttta
     5461 tttcttcttc tttttcctaa gattaccgaa aaacactttt ttagagtgat tcatatattt
     5521 ctcaagttta tcaagattta tcttccacaa aattattaca taaactataa gtaatataat
     5581 agcccatcta tattctatca tcaatatgaa atcaaaaact ccatgtaata aaattggaat
     5641 taacactgcc ataaacaaat actcacgtct tttattttta tttccctcaa atttatactt
     5701 agaaatatag tagcccatag taattgcaaa cattacatga gctggtatag agataactgc
     5761 cctattaata ccaacttgca gtgctagatt tctactttca gaaaaaatat aaaccatatt
     5821 ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa ataataccat ctaacttttc
     5881 agtaaagtgt ttttctttaa ttagggcagg aatcagtatc agagctttta caccttcttc
     5941 cacaagtcct gcaactacaa atgatacata tattagttct gaataccctt caaatacatt
     6001 aaacttcatc agtaaatttt ctaaaagtat aattataaca cttagaaaag cacctattaa
     6061 aaaaaattta attagatgga ttaggggttc tctacaatat ctatctttag accttatcca
     6121 aaacatccct atcaatatag gaactattgc taataaaaat aaatctagct tcatagatta
     6181 ttttcctttc aaaatttttg atttatttat gatttatatc atctacttat ttttagctta
     6241 tttgtgtttt aaatacatac taaaatatac attaaattat tttaaattat ttaaatacaa
     6301 atatactcaa atactcaaaa aaatttaaat ttatatctat attatttaac aaatttcaag
     6361 ctatattcat atcttgaaat ataagggaac taaattcatt taaagaaagg gtgatttgat
     6421 gcaagatggt ttcttaacag taagcataat tgatgctact aacaatagac ctattcaaaa
     6481 tgcagtagta aacatatatt caatgtctaa tggtagccaa agctcatcaa ctttatacca
     6541 aaacctaaga agtaatgaat ctggtcaagt gactggttta gtattaccag caccagatgt
     6601 agattactct ctacagccat ctgatgttag accttatagc cagtatatag tggaagccat
     6661 agcagatgga tatgagacag ttgtcataga aggaacgcag ttgttagcta caattgtagc
     6721 aagacaagga gttcccatgt ctcctagaac aagaagtaag cgttctttct caagacaaag
     6781 tgagttgatt tttgacatag gagaacatac tctatatggt acttatcctc ctaagattcc
     6841 agaaagtaac ttaaaaccat taccacctcc tactggtttc gttgtattag ataaccctgt
     6901 agtgcctgaa tttatagtag ttcatgatgg tttgccagaa gattcatcag ctccaaacta
     6961 ttggattcca tttaaagagt acattaaaaa catagcgtca tctgaaatat attcaacttg
     7021 gcctgaacag acaatctatg caaatgtaat agctataata tcatttactt taaatagagt
     7081 ctttactgaa tggtatagaa ataaaggata taactttaca ataacttcaa caacagcata
     7141 cgaccataag tttataaata atagaaactt atttgaacca attaacgtag ttgtagatgc
     7201 tatttttaat acttttataa aaagacctcc tacatcaaga cagccactat tagcacaata
     7261 ttgtgatgga caaaaatctc aatgtcctga ccaaatgact caatggggca gtaaagactt
     7321 aggtgaccaa ggatatgact atgagagtat cctaagatat ttctatggag atgagatagt
     7381 atttgaaaga gcacctatag taagtggtgt accagtgtca ttccctggta ctacactaca
     7441 agttggttct tctgggcagt atgttagaac catacaaaat cagttaaatg ctatatctaa
     7501 ttcttatcca gctgtaccaa aagttataga agatggtatc tatggagcag atacagaaaa
     7561 cgctgttaag atattccaag gaatctttgg tttacctcaa agcggagtag tagattttaa
     7621 aacatggtat gaaatatcca gagtttatgt agctacaact agaatagctt ctttaaatcc
     7681 tttaatttaa agcttgattt agaacataaa ctcaaattat tgaataaaat tattaattta
     7741 aaataaaaat ggctgttatg aactaatata taccataagt atttttactt ataagtattg
     7801 tagtcttaac agccattttt tagttataac ttgttttttt agaatcttaa aactttaaat
     7861 gggggatttt aagcttctta aaaacagtgc ttagcaactt agatttcttt aaaatattat
     7921 atttattttt aagtttaata aaatgctatt ctaacgaaaa gcttactttt tatttaaaat
     7981 ctcacttatt atttaaaatg ctacttctca cttagaatat tactccttat tatttaaaac
     8041 actatctatc acttagaata ttccttctta tctaaattat tatttattat aaatataaat
     8101 aatattaatt taaaaagtta ttcataattt aaataatact attatcttca tcagtgaacc
     8161 taaactgcaa ctttagactt attagctaaa gccaatatta gacctaaagc tatagatgca
     8221 aatgcagaag caattactct atactcagca gggaataaga atacaattgt atgacatggt
     8281 atccagaaaa atggtatagt ttttaaaaca ctaaaaccaa ggtaagcatt ccagtccaca
     8341 atatttacca catctttaag ttttacctta atcccctttc caacctcata ttttttatct
     8401 ataaaacagt ccataacctt atggaataaa aatacagttg gtgacattag tatattcata
     8461 gttgcactct taaatagagc atttgcaaaa gctattccct caaatggaag cataccatcc
     8521 acttgtgctt gacctacacc tgatgagtag attgacattc catatgtggt aactacacct
     8581 attattcccc aaactattgc tctataaaat actcctactg gtattttcca atccttatac
     8641 aaaattctag caccaagtaa atctcccatt gtagctaata cagcaaactt taaaaaaccc
     8701 ccaatatatg gatgtgcacc tgttgcttcc ataaatactt ctctactact tggtattgct
     8761 aagacagcaa taatacaaat aagaactcca ctccatataa aatcgccttt tttcatattt
     8821 ataaatacta tttaatatat aaatagttct cctttctatt aaagatttat ttaatttaca
     8881 atctatctac aattatttat taatcactta ttattagata ttcattttcc atatttaaat
     8941 ttattctatc cctttatcta tttattttct tactactcta tccaccatta tctatatatt
     9001 tattttacga ttactctttc accctaaccc tttaattatc tatttttttc tgatattctt
     9061 atctaccatc atccatatat ttattttacg attactcttt caccctaacc ctttaattat
     9121 ctattttttc tgatattctt atctaccatt atctatatat ttattttacg attactcttt
     9181 caccctaacc ctttaactat ctattttttt ctggtattct tatctactat tatccgtata
     9241 tttattttat gattactctt ttaacctacc ccctatactt tatagtaata tgtttttaac
     9301 ctttttcatt attcccaata tgctttataa ctacaaatac tctcccccct taaaaataat
     9361 aataaaatta atcaataaat ttataatcat attattactc tatgtattaa gcaattacta
     9421 tgccaataaa ttttgatttt tttaaacaaa tcttaaaaat cctaaaatgc taattttctt
     9481 taacttttaa tgaatctaat agataatatt taattttcgt tatatttttc agaatgtttc
     9541 aattattatt tttaagaaaa aaaaataatg ttaacatttc ttaacataca ttaaaaaatg
     9601 ttaacattat aaatttggtt taatactttt actttgactc gatttacttt tattctactt
     9661 cttactttat ttttaactta gactttattt ttttattact tatttgagtc ttatttattt
     9721 tattctcact ccatatgttc aattacattt gtctaaattc tttgatactt ttattacata
     9781 attttaacaa gcttaagtgt tatttgggta gtgcagttga taattattta aataatcatc
     9841 aactgcatgt ttatatcatt acagtagctt aatgtattta atcatactta cttaggattt
     9901 tgaaatatct tagagattat atttaacatt agtcaattgc tgcataattg taagcaattg
     9961 aaatttgaga cccatcttca ttttctatag tgataggctt cataattttt aatccttcag
    10021 tttttatctt ttctaattct tgttccattt tcttaattgt ttcctcaagg tctttttgac
    10081 ttacatcttt gactgcatta gggtctttag catagtcttt taattcttga atatgtttag
    10141 catactcttc ataagtataa tgctcaacac catacttttc caattcagta gggtcagtta
    10201 ttcctgattt tattagagat tcaagtttag gtaaagcaat attagatgaa tcatttccat
    10261 ttgcaaatac tgctccagtt cctcctagta ccattgcaga taccatcatt gcacttataa
    10321 gggatttttt tattttcata accaatcttc ctttcttaga gatttatttg ggtagtgtag
    10381 tttaaaatga ctattgcaat agttatcaac cacatcttta gactaccata gtagtcatat
    10441 ttgtgtcaag tctttttaaa aaacttttca ataaaaaata ctatctcaaa acaaaattaa
    10501 ttattttgag atagtatttt aattctaaat cttttatatt actaagattt atggttactc
    10561 tatccatgtt ctatattgtt ctttattaac tgctaacaaa ttcccttttc ttgatatgac
    10621 aaagcgatta tcattatagt tactttgctc aattgaaact tctcctgaat cagtataaag
    10681 tacatcctta ctattactag atttatttac atacgtttct tcactattct cagaattagt
    10741 tactttgctc atagatgatt cttcatgctc tctaaattca attacagata cttttgccgt
    10801 actaaaatga gtctctccat cacttttagc tttactaaca agaatatcag attctacttt
    10861 gggagaatat aaatcagttg atttactact tattgaaaat ccacttttat cactttgttc
    10921 attttttgtt aaaccaaaag attcaacctc attaatctct ttattttctg ttgcaaaaac
    10981 aagacctgtc ccacaaacta atacagttga aactaaaaaa gtaacataag ttgatttttt
    11041 aaatttcata atattaacta ttctcttttc tatttttgtc tttccaaatc cactacatat
    11101 tgtcattgaa aatggattat tctcctctgc tagacttatt agtgtggttg cataatcagc
    11161 cctatattct tcaccaagta tactcataac cttttcatca caactaaatt ctatgtcttt
    11221 atttagtaaa aaaaacatca cccaaactaa gggattaaac caatggacaa caagagctac
    11281 aagcataaat aactttaaca gattatcaaa gctattaata tgaaccattt catgagttaa
    11341 aacaaagtct aaagtttgat tattagcata attcatttgc tttggcaaaa taattcgtgg
    11401 ttttaaaata ccagatgtaa gtggtgtcct agtaaattca gatatgcaaa caataaatct
    11461 tctaaaacgg ctattcttac acatccattt tttaatgaac tcatgatttt ttatatgaat
    11521 tgaagtttta aaaagattaa ttattttaaa atatctaaag cataaaagta tgctaaacat
    11581 aatagtaact ataaaagaca aaatgcccaa catttccaac atcataatat tttcaatatt
    11641 agacattatt tcaaattcgt cagaattgtt tttacctgta gagtcactag ctttcttaag
    11701 agtataattt tctgttgatg tattacccaa ttctacatca aaagtcgatt caattatact
    11761 ataaatacta aaaccagaat ttaaacttac tggtattaga agtttaaata aaacaatcaa
    11821 ccacaataat acaaatacct ttttaggtaa ttttttaaat aaacatgctc ttataaaaat
    11881 aactattaaa agtaatactc cagaagtaaa actcatttct aacaatttaa tgaacataaa
    11941 tcttacctca ctttaatttt tcaacaattt gtttgagttt tgtaatctca tcacttgaaa
    12001 gattttcttt attaataaaa gctgaaagaa atttttctga agaccctcca aacattttat
    12061 taattaaagt ttctgtttcc tgtttttgta cttcctcttt gcttattaaa gctttacaaa
    12121 caaaatttgg ctcaattctc tctattgcac cttttgacac taattttttt ataacggtat
    12181 acgtagtgtt tctattccat cctgtttctt cttttaaaat ttttactaac tctccagcct
    12241 tgctttctcc tctctcccat agaacttcca ttactttaag ttctgaatca aataatttta
    12301 tctccatata aaatcgctcc tcaaatatag ttatcacaat agtcattact ataataaaaa
    12361 actatcatag tagtctatac tatgtcaatc atttattttt taataataaa aactgtcttt
    12421 ttaaaaaatt cttataaaag acagttttat caatgttata ctttaatgtt taattaaagc
    12481 catattttat ttaaatgtat aactatctat cttagtacat agtataagat ttttctttat
    12541 ctaatagtat atcctctaca tctgggtttt cttttactaa ttgtctataa aaattacaat
    12601 tagtaacacc tacatatgga attagccata gtagacctat tccaagagta gctattgaaa
    12661 gtatatacca tcctataaaa ctaaagttta gtacaaataa tttaagtaca tttcctttca
    12721 tcatatctaa actagcaccc attgatttaa atacacccat attatccttg tcttcacaaa
    12781 ttaagaaagt agcttgagaa acatataata gcaatatata aaccggtaac ataagaacaa
    12841 gtattataag aactagagtt attccagtag aaataatagt ccctattcca acattatcac
    12901 ttgataaata ttcaattgct ccactagtaa gtaaaactcc aaatgataaa aacgctccta
    12961 taattgtgat tataaaagaa taaatataaa caataattga ttcaaataaa taaaaaccaa
    13021 aacttcttaa ataaattctc ccacttacaa agatatctcc tatcttgact ttttcatctt
    13081 tagtaatttt caagaacata catgtaaatc ctgctgaaac gcaaaaagct attacagcca
    13141 ctacaatagt agtcaatggt tcaaaaggta atcctacaat tgatattaat actatcaatg
    13201 caatatatac tgccatagaa attgcacata ttccccagtt tccagataac tgagctttag
    13261 atactcgttt taattcttgc cttgatacca taaaaaacac ccctttcatt ttataaaaaa
    13321 acttttaaaa atataaataa tcttttaacg tataatatta atatatcata ttttcgacta
    13381 tataagatac aattattcac catgttttat caagatatgc cataattgta ttactagttt
    13441 tatatagtca acattaccaa atacatcaaa taaatttgat taattattca acaagtaata
    13501 aaata
//
LOCUS       DAEPOG010000109        13411 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEPOG010000109 DAEPOG010000000
VERSION     DAEPOG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.109"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            33..275
                     /locus_tag="KR432_002518"
     CDS             33..275
                     /locus_tag="KR432_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0641046.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            278..1474
                     /locus_tag="KR432_002519"
     CDS             278..1474
                     /locus_tag="KR432_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641047.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1594..2319)
                     /locus_tag="KR432_002520"
     CDS             complement(1594..2319)
                     /locus_tag="KR432_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0641048.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            2797..4896
                     /gene="nrdE"
                     /locus_tag="KR432_002521"
     CDS             2797..4896
                     /gene="nrdE"
                     /locus_tag="KR432_002521"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH0641049.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4909..5880
                     /gene="nrdF"
                     /locus_tag="KR432_002522"
     CDS             4909..5880
                     /gene="nrdF"
                     /locus_tag="KR432_002522"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH0641050.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            5956..6582
                     /locus_tag="KR432_002523"
     CDS             5956..6582
                     /locus_tag="KR432_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0641051.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            6977..7606
                     /locus_tag="KR432_002524"
     CDS             6977..7606
                     /locus_tag="KR432_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641052.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            7607..8389
                     /locus_tag="KR432_002525"
     CDS             7607..8389
                     /locus_tag="KR432_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641053.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            8382..9260
                     /locus_tag="KR432_002526"
     CDS             8382..9260
                     /locus_tag="KR432_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641054.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            9293..10252
                     /locus_tag="KR432_002527"
     CDS             9293..10252
                     /locus_tag="KR432_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0641055.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            10566..11258
                     /locus_tag="KR432_002528"
     CDS             10566..11258
                     /locus_tag="KR432_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641056.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            11268..12272
                     /locus_tag="KR432_002529"
     CDS             11268..12272
                     /locus_tag="KR432_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0641057.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            12520..13371
                     /locus_tag="KR432_002530"
     CDS             12520..13371
                     /locus_tag="KR432_002530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0641058.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
BASE COUNT         5279 a         1329 c         2376 g         4427 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagattta aaacatcaga taatagagtt ttgataccag
      121 tagtaatatt tttaggcagt atagttacag ctctttgtga tttaatatca agagtaattt
      181 tatcaccagt agaattacca ataagcacga taacatcttt attaggagct ccaattgtag
      241 taatgttatt ggtgaaaagg aaggataggg cttagacatg ttgaaaacta ataatttaag
      301 tgtaggatac aataacaagg tagttataag taatataaat gtagaagtaa aaaatggaga
      361 aatactatgt cttttgggaa gtaatggagc tggaaaaact accctattaa ggtccttatc
      421 taaacttata tcaccaataa aaggtgaaat atatttgaat ggtgtaaaca taaattgtat
      481 atcaagaaaa gcattatcaa aaaaaatggc tttagtactt acaaatagat tattaggtga
      541 tttaatgact gtacaagata tagtcaatat aggaaggtat ccatatacag gattttttgg
      601 gagtctatct aagaaagatt taataatggt agatgaagca ttggagtctg tagatgcctt
      661 acatttaaag aaaagatatt ttgatgaact aagtgatgga gaaaaacaaa aggtgttggt
      721 tgcaagagct cttgttcaag aaccagaaat aattatatta gatgagccta caactcactt
      781 ggatataaaa catagattgg agctcataaa catactgaaa aaattaagta aagaaaagtc
      841 aatatcagta atactttcat tgcatgaaat agacatagca ttaaaaagtt gtgataaagt
      901 tgctcttata aaaaataata aagtcatagc ttatggtcag cctgaagatg tagtagatga
      961 ggatattata aattcgcttt atgaattgga tgataaaaac tttaatagtc ttttaggttc
     1021 agtagaaatt agtaataaat ctaaaaatga agtttttata attggtggtg ggggtaaagc
     1081 aacaccaatt tatagagctt ttactaaaaa aggtataggt ctgtattctg gaataataca
     1141 tgaaaatgat attgattatg aaattggtag aactatgggg ataaagatgt ttacagaaaa
     1201 tccatttgag ccgataagtg atgagagttt cgatttggct attagaaatt taaatgattc
     1261 caagattata atagatacag gatttagtgt aggagagaca aataagagga atatagatat
     1321 tataaaagag gctttaaaac tggataaaaa agtttattct tttagaaata gagatgagag
     1381 taagaagtat tatgacagtt tggatagtaa gatagaacat atagataaag tttctcaaat
     1441 aatcaatagt gcagatataa ataatttatt ataatttatg aaataaaatg tacttatgtg
     1501 ctatagaaaa aagagtatag attataattt ttttgaaatt acaatctata ctcttttgtt
     1561 attttgttta tatatttata aaactttagc attttataga taaccaatag cttcctctct
     1621 agttaaaaaa aagtgaatcc ctgtagtcga ttctttccat ctattttcat taaaatcttc
     1681 agctacaatc gtttctccta ctctatatat aaaattttca tcagcatatg cacgagcact
     1741 tttaaaagat tctttaccat ctatactagt aattgacagt acttttgcct tatcacatct
     1801 acaagcattt gatgtagcag acactctttt tgcgtcgctg ggtattaata attgtaccat
     1861 tctgaaatta aaacattttt tatagcctat aaatgctcct gtttcaggac aatacaattt
     1921 aaaaaaaggg gtactttcat ctgatattac tccatttaaa gtagcatgtt ctaaattgga
     1981 attttctaaa ttagctcctc taatatctga gttagataag tcacaaaatc taaaatttgc
     2041 accacttaag tctgcatttt ttaatataga attttttaat gaacaatccg aaagataaga
     2101 attattaaaa atagagtttt ctaaatctgc tttttctaaa tttaaattga taaggtcaga
     2161 ccacgaaaag tcaatattac ttaagcagta tcctcttaag tccatatttt ctaatcttaa
     2221 ttcttttaaa tctgcctttt ctccagcatt attgttttta agccataaat aatgtttctc
     2281 aagtgttttt tctaattctt gtttagaaat agttttcatt aaaatcacct tcataaattt
     2341 atttttatat atgtagtagc aaaatagtca tatatttaat tatataacaa taaataaaaa
     2401 acttctaaat aatatataaa atatcatctt gaaagtactt ttttcaatat atcagaatat
     2461 atttttaagc tatttagaga taatttaaat ttttaaacag gacataataa gcttgtattt
     2521 ttttatattt gagttgataa aaatttcttt aaataaaaat aatgtcaatt ataaagtaat
     2581 aaaagtttag atataaaaaa atttgtgtat atactatatg tagtatataa tagataaagc
     2641 actgactata tgtagtgctt ttgtttttga aaaatactta ttgcatttct ataattatag
     2701 attaattttg ggatttcaac tgataagtac caaaagaaat aataagcaaa ttaagtgaaa
     2761 ttatggcaca agattatgtt aaaaggacgg taaataatga attggataga gcttaacaat
     2821 caagtgataa ttaaaaatga acaaggtaag taccaattgg aaaaggataa ggaagcctta
     2881 agtagttaca tagaagaatt tatacaacca aagttgagaa aatttaataa cctagaagaa
     2941 agattgaagt atttaataga tgaaggttat tatttaaaag aagtaataaa tcagtatagc
     3001 atagatgtta ttaaaaatat atatgatatg attgataaac ataagtttga atttcaatca
     3061 tatatgagtg caaataagtt ttatcaaaat tatgcattaa agagcaatga tggaaaggaa
     3121 atattagaaa catataatga taaagtgtta attgtagctt tgacactagg aaatggcgat
     3181 aaagatttag ctttaaactt ggcagataaa atagtaaaac aagaatttca accagctaca
     3241 cctacttttt taaatgctgg aagaaagagg gctggagaaa tggtatcatg ttttttacta
     3301 tctgtagaag atagtacaga aggtatatca tatgctatat cttcatcaaa tcacttatca
     3361 aagattggtg gaggagttgc actaaattta tctaaactta gagcatctgg cgaatctatc
     3421 aaagatatag aaggtgcagc tggaggtgtt gttggagtag ctaaaatgtt agaacaatca
     3481 tttagttact ttaatcaaat gggagctaga caaggttctg gtgcggtgta ccttactgta
     3541 ttccatcctg attttgaatt gttgatggat actaaaaaga taaatgcaga tgaaaaaata
     3601 agacttgcta cattatcatt aggagctata ataccagata aatttatgga gttggcagaa
     3661 aaaaatgaag tagcatatgc attttatcct catactgtgt ataagaaata tggagtttct
     3721 cttgatgaga tagaaatgga tgagtggtat gataaattgg ttaatgatag tgatataaga
     3781 aaaaaagaaa taaatcctag gcagatgttg acaaaaatag cacaaatgca acaagaaagt
     3841 ggatatccat atgtagtgta catagataca gctaatagag aacatacatt aaaagatgtt
     3901 ggaattatta aaatgtctaa cttatgttgt gagatatttc aatatcaaac accttcagaa
     3961 atagaggggt atggaggaaa aaatgaatgg ggacaagata taagttgtaa cctaggttct
     4021 ttaaacatag ccaatgtaat ggataataaa actatagaaa gtacagttga aacagcaata
     4081 agagcactgt catttgtagc agatagcaca gatataaaac cagtgccaac ggtaagcaat
     4141 agtaacagca agtctcattc tataggatta ggagctatga atttacatgg atacttagta
     4201 agagaaaata ttttatatac ttcagatgat gctatagatt tttctaatgt attttttgca
     4261 atggttagat actattcaat taaagcttct atgaaaatag ccatagaaag aaatcaaact
     4321 tttgaaggat ttgataaatc agaatatgtt aagggaagaa atagcaaggt tttatcaaaa
     4381 tactatgaac aatcttatct acctaagtca gaaaaggtaa gagcattatt tgaagggatt
     4441 tatataccaa caaaagaaga ttggacaaaa ttattggatg aagtaaaaga aaaagggata
     4501 tataatgcat atttaatggc agtagcacca actcaaagca ttagttatgt tcaaaatgct
     4561 acatcaagta taatgccaat tactgagcca gtagaagtaa gaacatatgg agactctact
     4621 acaatatatc caatgccatt tttaacaaat gataatatgt tgtattatca atcagcatac
     4681 aggatggata tgagaaaggt catagattta gtggcaacag ttcaaaacca tgttgaccaa
     4741 ggtatatcaa caacactgtt tgttacagat gaaaagacaa ctagagatat agctagacat
     4801 tacatatatg cctataaaaa aggtttgaaa agtttatatt acacaagaac aaagatgaca
     4861 agagatactc atgagtgtct agtgtgttca gtatagggag agaaatatat gacatttaat
     4921 ttacataaaa tacataatgc agttaactgg aatagagaag aagatggatt tacacaagct
     4981 ttttgggaac aaaatgtaaa acaattttgg cttcctgaag aaatatctgt ttctaaagac
     5041 ataaaggtat ggaatgaact aagtaataaa gaaaaggaat tatataaaaa agtattgggt
     5101 ggtttaacat tactggatac aaaacagggg aataatggaa taccatctat gatgagttta
     5161 acagaaaacc tacaaagaaa agctgtcttg tcttttatgg ggactatgga agaaatacat
     5221 gcaaaaagtt attcttctat atttatgact ctactttcta atttagaaat agatgaatta
     5281 tttgaatgga tagaaacaga accaacatta caaagaaagg ctgatttagt tttagctcaa
     5341 tacgaaaata ctacaaatca agagggatta tacctatcaa tggttacaag tgtattttta
     5401 gagagtttct tgttttattc aggattcttt tatccattgt atctatctgg tcaaggaaag
     5461 atggtagcta gtggagaaat aatatcgctt attcttagag atgagtcttt acatggtaag
     5521 tatataggat tgctagcaca agaaatatac gattcatttg ataaaatgga taagaagatg
     5581 ttagaagaga agatgtattc tatactttat agccttatgg agaatgagat agagtataca
     5641 aatgttatat acagagaaag tgggcttgaa aaagaggttg tgaacttttt aaaatacaat
     5701 gcgaatagag ctttagaaaa tctagggttt gagaagctat atactgtaga tgctataaat
     5761 ccaattgtat taaatggtct tagcactgaa accaaaacac atgatttctt ttcaactaag
     5821 ggtaatggat accaaaaagg agtgtatgag gaattagagg atgaagattt tataatataa
     5881 tttataatta tgacacactg ttgtcacttt tagcgtgata atatcaatgt ataaaatata
     5941 tatggaggta tttttatgaa atatgaatgg agaaaaagag ataaagagtt atatttacct
     6001 aaaaagaatc ctgtaataat tgaaatacca gaacagaaat ttatcatgtt aaaagggagt
     6061 ggagacccaa attctaaaga atttattgaa gcaataggag tactttattc tttggcatat
     6121 gcaataaaaa ccatgcctaa aaaaggaatt gtaccagaag ggtattttga ctatacggtt
     6181 tttcctttag aaggtatatg ggataaggga gaaaaaagta aaggcttaga tgtacttatt
     6241 aaagaagatt taatttacac tataatgata cgtcagccag attttgttac acaagacctg
     6301 ttggataaag ctactgatat agtaaagaag aagaaacctc ataatcttct tgaaaaagtt
     6361 aggtttgggt ctattaatga agggctatgt gttcaaatgc ttcatatagg ttcatatgat
     6421 aatgaaccag aaagttttga cattatgaac gaattttgca aaaaaaataa acttataaga
     6481 aaatcttaca cacatcgtga aatttatata agtgatgcta gaaaagtatc gcctgaatca
     6541 ttgaagacgg ttttacgttt tgaagtagaa agggaagtat aaatattttg aataatttat
     6601 aagagttata aaaagtgaag tctgttgaga cttcattttt tgatgcaatt ttaaaattaa
     6661 atttgatgtt tcataatatt ttaaaaactt tctactttta ttagtgtgaa taactgttgc
     6721 tattaaataa taatactaat tatataagta ttattatata atatttatag attattatat
     6781 aaatagatta ttagtaagat gtatgcatta ttaattaaaa taaattacat atactattta
     6841 atatttcatt taaaatatta aaaaatgtag ataaaaattg ttttattaag gaaaaattaa
     6901 tatattgaca aaatttaagt gctcatttaa actattaatt gataataata ttcaatatta
     6961 aataatagga ggcgttatga ataaatatct tctaataaat ccagtggcag aaagaatgta
     7021 tggagaaaag tttcacctta ttaaagcaaa tttgataaaa aaggggtaca taatagcaga
     7081 atgtgaacca cagttagaat atgtaaaaaa gcagtacaaa gagtatacaa aaaagactga
     7141 aaatacaatg ttggattgta gatgtccaga aagtataagt cttttaaaaa gaaataattt
     7201 gataaataat tttgaagtac ctatgataga accaatatta gtaaggactt gtagggtttt
     7261 atacgataaa tatataaaaa gtaaagatga tatgttggta gtaacctgtc catgtacaca
     7321 attaagagac tttgcaagtg aaaagtttaa ggataagggt aatgctatgt tttatacatg
     7381 gaaagagttt gctgaacaag aaggtgtaaa atcacttgga aaaatagatg aatcaccaat
     7441 tccattagga tatttcaaag atacttttga aaaagttctt gaagtatcaa gtgaagacga
     7501 aatattaagt gaaatacaaa aaataagaaa tggctttgaa gataagtatg acatagtaga
     7561 aatgttttat tgtaaagatg gttgtaataa tggagatggt ttgtaaatgg tcaataaatt
     7621 taataataag tttttaaggc tagtaaagag tgttctgata ttttttataa ttcttttatt
     7681 atggaaaatt acaaattatc ttggtatttg gagtgattat atattaccat cacccgaaaa
     7741 ggtatatagt acatttttaa atatgataag tgatggttca atatttatta atgtatatgc
     7801 aagtatgaaa agagttttga taggttttgc aataaatact gctataggtg tgcctttagg
     7861 gatattcttt ggaatttata gtggagtata cgaatacttt aaatctctaa taaatttttt
     7921 aaggaataca cctccccttg ctttaatacc aatgcttata ttatggtttg gtataggaga
     7981 ggaatcaaaa attataatta tagttttagc atcctttttc ccaatcttta caagcacatt
     8041 gaaagggatt aaaaattgtg actcaaaact gattgaagtg ggtagagtat ttgagttttc
     8101 aaagcttcag ataatattta aaataataat accaaatgcc attcttgata ttgctgttgg
     8161 gttaaagtta gctcttggat atagctttag agcaattatt ggggcagaac ttgtagcagc
     8221 ttcatctggt cttggatact tgatatctga tggaaaagaa atgtctagaa cagatgttgt
     8281 tattgtaggt ataatcgtaa ttggactttt aggaataatt acagattaca tattttcgat
     8341 tattgtgaag aaggtaagta agggaaagat ggtggaagca tatgaataat ttagtatgtg
     8401 actctggata tataattaga aatttatata aaagttataa agttgacaat gaagaacatg
     8461 tagttcttag tgatatctct ttaaacatag atagaaacca tataacagta attttaggtg
     8521 aaagtggttg tggaaagact accttgctta gagcaattgc atcacttgaa ggtataaata
     8581 gtggtgagat taggtttatt aatgataata aagagtatag acctaatgtt ggttttgttt
     8641 ttcaggagag cagattaatg ccttggttaa acgtaagtga aaatatagct tttcataatc
     8701 aaagaaaaaa tactatttta aacaaggtgc ttagaaaatt taaacacaat aaaagaatta
     8761 ttggtaaaat tataaatgat aattttagaa gcttagaaac aaatgataat ttgaacaaat
     8821 caaataaata taataggaaa agtaatattg atgtagaaaa atatcttaag atgatgaact
     8881 tgcagaaatt taaaaactct tatccaaatg aattgtcagg aggtatggca cagagagtaa
     8941 gcatagctag agctctttct ttcaatccag atatgttgct tatggacgag cctttttcgg
     9001 cattagatta ttttacaagg attgatatgc aaaatgaagt tataaaaata cataagtcaa
     9061 ctaataaagg cgtaatattt gtaactcatg atattgatga agcgttaaag ataggcaaaa
     9121 aaatagttgt tttcacagac gaaagaaaaa taaaagaatt taatatagaa gatggttata
     9181 atagagattt aacttcagag tactatatta aattaaaaaa agatatttta atgacaatga
     9241 aatcaaataa agacatttaa agaaattaat aatattttaa ggagtagagt aaatgaaatt
     9301 aaaaaagcta ttgataggta taacgatatt aacacttgca acagcatctt tagctggatg
     9361 tgctaaaaaa aataatggag aaaaactttc agaaataaat ttaacttatg taaaatcacc
     9421 attaaatgtt ccttctataa ttcagaaaca ggatgattta tttggaaagg aatttaaaaa
     9481 ggataatata aaagttaact ttcacgaaat tactacaggt ccagagcaaa cacaggcttt
     9541 agctgctgga gaaatagact tcttacatgc actaggtggt acgtcagctt taatagctgc
     9601 atctaatgga gtagagttaa agatattaaa cacttatagc aggtcaccaa aaggatttat
     9661 gattttgact aataataatt caataaaatc agcagcagat ttagtaggta agaaggtggc
     9721 aggtccaaaa gggactatat tacatcaggt attgatttca gcacttgata aagaaggatt
     9781 aagtatggat gatgtggaat ttgttaatat gggtattcca gaagcatcag cagctctttc
     9841 tgatggtagc gtagatgttg cattgattgc tggtccagca gctcttaaag ctatgaagtc
     9901 aggtagtaaa cttgtagcaa atggtgaagg tttagtagat ggtataatag taactgctgt
     9961 tagcacagac tttgcagaga aacacccaga aatagttgaa agatttatga aggttgaaaa
    10021 ggaaacttta gaatatgtaa ataataattt tgatgaagct atggaaaaag tagctaaaga
    10081 agttgattta agtcttgaag aaactaaaga attatatgct tggtatgatt ttagtttaga
    10141 tataacagat aaagatatat cttcgcttga agatacacaa gacttcttaa taaaaaataa
    10201 gttacaagag aaaaaagtga atataaaaga attaatctat aatgctaagt agaaactatg
    10261 ctaaaataat actaagtaag aaatataata aaagtttaag ttaccttatt aaggtaactt
    10321 tttctatttt gaattagtaa tttcaattta ttttagaatg attagttgat aataatgatt
    10381 aattaatcat aaaaagatac ttttttatag agttttatta agatgtctaa taatatggtt
    10441 atacttatga agtcagtaat tgtgaagtta ataatatgat taaatttata tattgtaaaa
    10501 agattataat atattaaaat tggactgtta tatgatgagt aaataggtat attaggagga
    10561 attatatgag ttataaagta tatattgtgg aagatgatat atcaataagt acattactta
    10621 aagattatat tgagaaatat ggatttgaag tgattgttga agagaacttt gatgatataa
    10681 tgattacatt tgaaagtttt aagccacact tagttctatt agatgtaaat ttacctaaat
    10741 ttgatggttt ctactggtgt agatgtatta gacaacagtc gaatttacct ataattttta
    10801 tatcagctag agatggcaat gtagaccaaa ttagagcttt agaaagtgga gcagatgatt
    10861 atataacaaa acctttttac tatgatgtag taatggcaaa aataaagagt catataagaa
    10921 gggtatatgg tgaatattca cctaaaatag acgaaagaat agtggagcta gatggattaa
    10981 agttttatcc agaaaggtta gaactagaat taaatggtga aaatctaatt ataactaaaa
    11041 aagaaggtat actgattgaa tgtcttatca agagataccc aaaagtagta agtagagatt
    11101 atcttttaga aaagatatgg gatgatattg agtttgtaga agaaaatact ttaaatgtaa
    11161 atgttagcag gataaggaaa agatttgagg aattaggtat agataatgct gttcagactg
    11221 tccgagggtt aggatataaa ttaaataaga attggtaagg agaaagtatg aagttatttt
    11281 taaaagatta taaaggttat atttttatat attatatagg catagttatg acaattattt
    11341 actgtaatct tatgaagttt attagttttg gagagagttt atatattctt ttatttagtt
    11401 cttttatatt gttatgtttt ttaatatata agtattataa aaatagagaa gtatataatc
    11461 tgtttgaaaa aggaatacat tctttggagg agtcattttt gtatttagga agctcattct
    11521 tggggagaaa tatatcagtt attttaaaac aacaacataa attgtatcaa tcaatgatac
    11581 aagagcataa aaagatacat agtgaacatt taactttcat caaccaatgg attcatcaga
    11641 tgaaaacacc aatatcagtt ataagccttc agcttcaaga ttatgaggga gaagaagtaa
    11701 ctgaaaatat aagaaaagaa atagatagat taaataaagg gttagatatg gctatgcatt
    11761 ttgcaagggc agataatttt cagaaagact ttatagttga aaaagccaaa ttaaatgaaa
    11821 ttgtatctag tattgtaaat gaagaaaaaa ggttatttat aaaaagtgga atgattccta
    11881 agattcaagt agacaaattt acttatgttt atacagatgt aaaatggatg aaatttataa
    11941 taggtcaatt aattacaaat ggagtgaaat attctaaaga ttatagtaac tacctaacta
    12001 taaaatctga agaaaataat aaatatgtaa ttttaagtat aatagatgaa ggaattggaa
    12061 ttgtttctaa agatttaaaa agggtatttg acccattttt tacaggagaa aatggtcgta
    12121 agtttggaga atctacaggt atgggtcttt atctagtaaa aaaggtatgt gatgatttag
    12181 gtcataaagt aagtataaaa tcagaagtag gaaaaggtac agaagtttca atctacttta
    12241 ataaagatgt aaatagtttg ataaagatgt aaattatctg ttacaatagg gtttttagtt
    12301 aattgtactt ttttattata caatttatta aatgaaatat tcatagttaa ataatcaaaa
    12361 tttaagagag tttatttgca aaccttacat agatgtaagg tttgtgtaat gtttgcagat
    12421 agttaaattt tctaatctaa attacaatta atataagaaa taacatgaga gaaataacat
    12481 aaaaaaagaa attttgtaaa aaaggaagga tgtttattga tgagtttaga tgttattttt
    12541 atattgaaat cagtcataat agctatagta gaggggttta cagaatttat tccagtttca
    12601 tctacaggtc atatgatttt ggttggtaat ttaatagatt ttaaggggca atttgctgaa
    12661 atgtttgagg ttgtaataca acttggggct atattagcag tagtagtact ttattggaaa
    12721 aagataaaag atagcgttat agaatttttt aaatttatat ttactggcgg aaaagaagga
    12781 aagattggtt ttagatttgg aatgaatgta attataggtt gtataccatt tgcgattata
    12841 ggagttctat tttatgataa tataaaatca ttgtttaatt tacaatctgt aattatagga
    12901 tttatagtgg gtgggatttt attattagta gtagaaactt tgtttagaaa aaagaatcat
    12961 tcaacagata atatagataa aattactcca atacaagctt taaaggttgg gacattacaa
    13021 gttttgtctg catggccagg tatgtcaagg agtgcatcta ctattatggg tggatggata
    13081 gcaggactta attctcctac agcagctgag ttttctttct ttttagcagt accagctatg
    13141 gtggcttctt caggaaaaga tttatttgaa tttgattatt caataatgac accaacattg
    13201 tggattgctt tggtagttgg gtttatagta gcttttattg tatcaataat agttatggaa
    13261 aaattcgtga attttttaaa gaaaaaacca atgagagtgt ttgctgtata cagaataatc
    13321 atgggtgtag tacttgcagt attagctttt actaacataa ttagtgtata gaaaaaatag
    13381 atttattatc tataaaaatt aaactaagat a
//
LOCUS       DAEPOG010000110        13244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEPOG010000110 DAEPOG010000000
VERSION     DAEPOG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.110"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            203..928
                     /locus_tag="KR432_002531"
     CDS             203..928
                     /locus_tag="KR432_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641059.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            915..2159
                     /locus_tag="KR432_002532"
     CDS             915..2159
                     /locus_tag="KR432_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0641060.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            2239..2916
                     /locus_tag="KR432_002533"
     CDS             2239..2916
                     /locus_tag="KR432_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641061.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            2917..5478
                     /locus_tag="KR432_002534"
     CDS             2917..5478
                     /locus_tag="KR432_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641062.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            5699..6973
                     /locus_tag="KR432_002535"
     CDS             5699..6973
                     /locus_tag="KR432_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0641063.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(7134..10175)
                     /locus_tag="KR432_002536"
     CDS             complement(7134..10175)
                     /locus_tag="KR432_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBH0641064.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            10382..10681
                     /locus_tag="KR432_002537"
     CDS             10382..10681
                     /locus_tag="KR432_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH0641065.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(10919..13147)
                     /locus_tag="KR432_002538"
     CDS             complement(10919..13147)
                     /locus_tag="KR432_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBH0641066.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         4971 a         1615 c         2014 g         4644 t
ORIGIN      
        1 tgtaagttgt aacttaactt gaaagaatca gtatcattct ttagatagtt aagttacaac
       61 ttacattttg tttttataaa ttttaatagt tctttgtgac tattttgtaa ttttagaaat
      121 attttagtat ccagggtaat tgtgtataat aaaacttgta tagatagata gtatgaaatt
      181 aagcagatag tttaaaatta aaatgaaaag aaaggaaata gttatgttaa agttgttgat
      241 agttgaagat gatagtacta tatcttttgg aataaagtat gctttagaac aagaaggatt
      301 tagtatagat atatcaaagg atttatctag tggaaaagaa atgatttctt ctaatgaata
      361 tagcatgata ctgttggatg ttacattgcc agatggaact ggttatgagc tttgtaaata
      421 tataagagga ttctctcagg ttcctataat attcttgact gcttgtgatg aagaagtaaa
      481 tattgtaatg ggtctcgata taggtggaga tgattatata acaaaacctt ttagaattag
      541 agaattaata tccagaataa aagctgtttt aagaagaaaa ggtaatactt cagaagaaaa
      601 taagaaaata cttaaatttg gagatttaag tatatataca ttagaagcta gagtatataa
      661 aaatgataaa gaaatatttc tgacatcagt tgaatataaa cttcttttaa ttcttataca
      721 gaataaaaat actgttttaa gtagaactca aatattggaa aaactttggg atgtaactta
      781 tgattttgta aacgacaata ctctaactgt ttatataaaa aggcttagag aaaaaatagg
      841 tgatgattta tgtgaaccaa tctacataga aactgtaagg gggattggtt ataaatggat
      901 aggaagtgaa aatagtgttt ctatataatc cagaagtgaa gaagttttta agtaagtatg
      961 ttactttgat gtttattgtt atcataatat ctattggatt tagcataatc aacgtgtctt
     1021 taactaaaga gatggttgta agaaataatc aagctataat agggacttta tcgagtaagt
     1081 atccaaactt agaaagtcag atagtagaca taataactca agggaagtct atgaagaaca
     1141 tagattatgg tcagaaaata ttaagtaagt ataattatga taaatctatt aagataaact
     1201 ctgagcctat tatttctaaa ttagttttag agactatcaa aataaatata atattagtat
     1261 gtataatatt tattttgata tttgtgttgg ttgttagata ttttacgtca atttacaatg
     1321 atttatcaga tatgactaag tatgtttact atagttcaga gggtaagcca tttgatatga
     1381 aaaacaagaa tcaagaaggg cagataggtc ttctaaaaac tgaactctta aagatgacga
     1441 caattcttaa tgaaaaggtt gaacttttaa aaagagaaaa gatattttta aacaatacaa
     1501 tttccgatat atcacatcaa ttaaaaactc ctatgacttc tttaataatg ttgaatgatt
     1561 tattatataa tgatgtacca tacgaagtaa aaatcgattt tttaaataag ataaaaaatc
     1621 aattaaatag aatggattgg ttgattaaaa gcatgcttaa attatcaaag gtagaagcaa
     1681 aagtgataaa ttttaaaaaa gataaggtaa aatttagtga actcatacat agagcaatgc
     1741 tatctatgaa aatacctatg gagattaaaa atcaaaaact gactatagag ggaaatgata
     1801 atgtatctta tattggtgat attgattggt ctgtggaagc actagtgaat ataatcaaaa
     1861 attgtgtaga acatactcct gaatttggaa atataattat aacctataaa gaaaatccat
     1921 tattttctga actaataata aaagatgagg gtgaaggtat agataaaaaa gatataccac
     1981 atgtatttaa acgattctat agaggaagaa gtagttcaaa agaagatagt gtaggaatag
     2041 gtcttgcaat gtcaaaatct ataatagaaa gccaaaatgg cgatatatat gtaaacagcg
     2101 aaaaaggtaa gggtacagaa tttcatataa tatttcataa aatgtatgat agtgactaat
     2161 ctgtaatatt taattcactt tatagtaata gccaaaacct aatatatgaa gtatagaata
     2221 taaatattgg aggtagttat ggaaatttta agagtagaaa atctaactaa aagttatggt
     2281 aaaaatgaaa caaaagttga tgctataaaa aatgtaagtt tatcagttga aaaaggaacg
     2341 tttatagcaa taacaggtcc aagtggaagt ggtaaaagta ccttattaca tttattagga
     2401 ggagttgaca gaccaactag tggtaaggtt tatataaatg atgttgatat atataattta
     2461 aaaactaagg atttggcgat atttagaaga agaaatatag gtcttatata tcaattttac
     2521 aacttaattc cagtcttaaa tgtgaaggaa aacatattgc ttccagcaga gcttgacaat
     2581 agagatattg atagagaata ttttgatgat ttaatgaaaa cattaggtct aattgataga
     2641 gaaaagcacc ttccaaacca attatcagga ggtcaacaac agagaacctc tataggacgt
     2701 gcattaatta atagaccttc tataatatta gcagatgagc caacaggtaa tttagatagt
     2761 aaaaattcaa aggaaatatt agaattgtta aaattgtcag taaaaaaata caatcagaca
     2821 ctaataatga taactcatga caaaaatatg gcacttcaag ctgatagaat tataagcata
     2881 gaagatggaa tcataaaaag tgatgaggtg atgtagatga atttatatac atcactaacc
     2941 attagatact taaaacaaaa taaaagaaga accattgtaa ccataatcgg tataatactt
     3001 tcaacagctt taatttgtgg tataggaaat atatttgaaa gttttatgga ttatcaaatg
     3061 agagaaacta taaagaatga tggaagtttc catgttacat tttatgatgt aaatagaaaa
     3121 aatgtggaat atgtaacgaa atcagctgaa atagaaaaac atgcatttac caaacaactt
     3181 ggatattcta aattagaaaa tagtgaaaat gctatattaa gtataaagca atatgatcaa
     3241 aatgcaataa acggatataa agtttctata aaagaaggta gatttccagc taaggaagga
     3301 gaaattgttt taagtgaaag tattattaat cttatagatg ataagttaaa aataggagat
     3361 aagataactt taaaagtagg ggatatgttt gactcaagca agaaaaagat tgatagtatg
     3421 acatttcatg aaggtgatta tatagcaaat gaaaaagata gaacttttaa agttgttggt
     3481 ataattaaaa aacctgggtt tgaacgttat aatggaattg caacagctat agcctacttt
     3541 aatcctatga ataattacaa tgatacaatt aatgtatcag tagcagtaag aaatcctaaa
     3601 gatgtgtata agataagtaa taaaataagt aaaaatatat attcaaataa agataaagaa
     3661 gcagtttctg atatgataaa atacaatgaa catttattga ggttacaagg tgctagtaag
     3721 tattcaaata taaatagctc tataaagtct ataattatag cagtaactat tttagtaatc
     3781 atatgtacaa ttgctacagt gtacaattcc tttagtattt ctataaatga acgaaaaaaa
     3841 cagtttggga tattaaattc tataggtgct acatctagtc aaattaaaag attagttttt
     3901 atagaaggta ttataattag tttaattggt ataccaatag gactaatatc tggaacagta
     3961 gctatagacc tattgtttaa aattataaat aagtatttta ctgaatcggt tgttactaag
     4021 atgagtttgc agatagtgta taatccaata ataattattg ttagtataat aatagttttg
     4081 cttacaatct ttatatcaat tctactacct gcaatatcgg cttctaatat ttctcccctt
     4141 aatgtgatta aaaatactgg agagtataaa gtgggcaaaa tcaaaagctc aagacttatt
     4201 aaaatgattt ttaaaactga aggagtactt gcatataaaa atattaggag aaatagaaaa
     4261 aaatttataa taactctttt ttctttaatg gtaagtataa taatatttat atcttttagt
     4321 ggctttacat tacttttact aagaggtgaa gaaattagaa attctcaaag aaaatatgat
     4381 ttgtatttaa ctgcaaagaa cacagctagg tctgtagagg atactataag tgaactagaa
     4441 gatattgatg ggataaaaaa ctttgaattg gcaacaggac aatatgtgtc tataagagta
     4501 agtgaaaata aaataaataa atccaaggaa gatttaatca ggaaatatta tcaaaaatat
     4561 aagattggag aaagttatga atatgatttc agtaataatg aattgatttt tccgggagat
     4621 tttgcagtaa aaaacaaaat aaataactta gtacagggct cttttaataa agagagagca
     4681 attgaagaaa atggtgttat attagttaga aaaagtgcaa ttgaagaaaa tgggaagaaa
     4741 ggtgtagtag aacttaccaa ctataaagta ggagatacag taaattgtga atatcttgat
     4801 gaaaatgcct taagtaaaaa agtaaaggtc aagattcttg ctataactga tgaagaacgt
     4861 ctaggttttg gttatcaaaa tatggggtta caatttataa cttatgatga agttgctaaa
     4921 aatttaaatt taaaattaag taaaagcctt atatttattg attcaggagg aagcattaag
     4981 actaagaagg aaatagaagc tttggcaaat aaaaatgatt ttaactttta tgatgaaatt
     5041 tcaagcagtg aaagtgaaaa acaagattta aaggttatta aaatatttgt atatggtttt
     5101 atagtagtga tatctttagt gagtgttaca aatattctaa atacagtaag tacaagtata
     5161 aatctaagaa aaagagaact tgcagtaata caatctatag gggttactcc taaaggattt
     5221 aggaaaatga tatatttaga aagttttata tatggtatac tgtcattgtt gtttggaata
     5281 ccaattagta ttggtataat tctaattatg aataaactta tttcaggtgt aattgaattt
     5341 tctccgatta taccgtggac agccatagtt atctgtatag taagtgtttt tataataact
     5401 tttatagcag ggtatatacc aatgagtaaa ttaaataaag aaaatataat agataatata
     5461 agaagagaaa gtatatagat ataaaaataa ttcttaacta tataaaatat tattaaaatc
     5521 atttaagtgg tataaaatat tattatatca cttaaatgat tttttattta tataagaaat
     5581 ctaataataa attactagag gtatttaact atttagaaag aattggtatt atataaagta
     5641 agagaatata taataagaaa gaatatttga ttttatttga aaacaaaaga aaaagaaggt
     5701 gattactatt tctcatataa ctgcgaagaa tatgtataaa agtttagagg aaagaataaa
     5761 caagtttcca caaggtgctc caccatctga tacattatac aaaatactaa atgtactata
     5821 tacagaacaa gaggcgaagt tagttgctca gcttccaata aagcctttca gagtaaaaac
     5881 agcagctaaa atttggagtg taagtgaaag tgaagcatat agagtattgg ataaacttgc
     5941 tagtaaggct ctgatactag atatagaaga taataaaggg aagaaatata taatgcctcc
     6001 tccaatggct ggtttttttg agtttgccat gatgagaacg agacacgata tagaccaaaa
     6061 acttttggca gaactttact atcaatatat gaatgtagaa gaagatttta tcaaagattt
     6121 attttattca acagaaacga agcttggaag agtatacgtt caagaggaag ttttaactaa
     6181 tgataatgag gttagtattt tagattatga aagggctact catataattg atgaatcaac
     6241 tcatataggt ataagtatgt gttactgtag acatagaatg caacatgttg gaaaagcttg
     6301 tgatgctccc atggatatat gtatgacgtt tgacaatgta gcaaattctt taataaataa
     6361 taaatttgca agacgtgtgg ataaaataga atgtaaagaa ctacttcatc aagcatatga
     6421 acataactta gttcaatgtg gagaaaatgt taggaaaggg gttacgttta tatgtaattg
     6481 ctgtggatgt tgctgtgaag caatggtagc agctaaaaga tttggaaatc ttcatccagt
     6541 acagacaaca agttttatac caaatataaa ccatgaaaat tgtgtaaagt gtggtaaatg
     6601 tataactgca tgtccaatag atgcgataag caaggttaag gaagatggta aagaatatat
     6661 aaaaattgat gaggatagat gtttaggttg tggagtttgt gttagaaatt gccataaaaa
     6721 tagcattatg ttattaaaga gagatgaaaa aataataact ccagcaaatt cagtacatag
     6781 agcagttctc atggcaattg aaaaagggca gttacaaaat ttaatttttg ataataatgc
     6841 tctggctagt catagagcta tgggagctat actgtcagct atattaaagc ttgaaccagc
     6901 taaaaagatt ttagccagta aacaattaaa gtcagtatat ttagataaat tgttaagtat
     6961 gaatgataaa tagaaactaa cttaaaaaag gcaaagtttt agacttatct atatcttaat
     7021 aaacttatca tttaatattt aaatttaatt tttacacata taaaacttat aacttaaata
     7081 aaaaaaggct atattcaatt aatattactt gaatacagcc ttttttagtt atgttaagct
     7141 atttctttta tttgcttaaa tgagttttca tttccatttc caccaatttg tgttacagat
     7201 ttaaatctca tagttttaaa tagttctttt tgattataat ctagtgagtt tcctactagc
     7261 attacaggag atttagtttt tccagccaaa actccaacag ataatccgtc tattaaatca
     7321 tcttgtttat tcattccatt tttagcaaca tataaattgt ctatcttaga atccttgtaa
     7381 aattctttta ttacttttgc attagtttca tttctagtgt tacctgatat tctctgtgga
     7441 ttttgaagtt tactttctat atttttagaa actgtatatt ctccaccgat tacataagat
     7501 ttatcaatct ttttgttttt aaataagtta tttatttcag ttatattact attttcatta
     7561 gtaagtatta taggcatatc attttgagct gatacagctc caatgcttac tgcatcggct
     7621 aatccttttt cgccattaac tactgaaatc tttgatatat tgctaatatt gttcaattct
     7681 ttagcaatgt tcatagaagt ttgatatcta tcacttcctg aaattctaga tacagagata
     7741 ttgtttgatt ttatagtatc taaagatttt tcatttatag aagcctctcc accaactaca
     7801 tatacatttt taacttttaa tctctttaat tcagccaaag ttttttcatt tatttgagag
     7861 cttccagtca ataatatagg agcgtttttc ttgtatgcaa atggtgttgc ggccaaagca
     7921 tctgcaattg ctgaatcgtt tattaaaact gcattttcag cattttccca acctgcttga
     7981 cttatcttaa tagaagtttc atatctatca gttcctgtaa gtgcacttgc atttacttca
     8041 tcttgatatg taacctcaaa tctagcattt ggacttccta agaaaacaat tacgcctcct
     8101 tttggcaatc taactctatt gttctttgta actaaagaat agtttacagg aacaccttta
     8161 tcatcataaa cagcaaaagc tgaattttga ggaagattaa cttctatttt tttatttgcg
     8221 atatcgtcac caatcttata ccattttgca taaccattgg attctagttc acaagtaaaa
     8281 gatttttctg ctggcaaatt agttatagaa tcttcactaa catacgttag tgttccaaaa
     8341 gataaatact ctgtaccatt ttctttatga agtttaatgt cggataaatc acgacctatt
     8401 acaccaggta tttctataaa agcatttgag ttattctcat ccattatttt agtgttaact
     8461 atataacctg gagtttcatc agaaagacta aaacttgctt ttacagagcc aaacatatag
     8521 ctttgagaag tatatttttc atccactaga tagtaacctt tgccacttct ttttttccac
     8581 acttctttga cactatttga tatattgttg tcatctactt tttgagctac atagtataaa
     8641 cttgctgtct gacctaatcc agggacatca tcataagagc tcatatttaa atatgtgata
     8701 ttattttttt cttttacaaa ttttaaacaa ctgttaccct tttcagatac aaatctatct
     8761 tgacctatgt atacgtattt atcttcaggt ccattttcta tataaggaga agatactgtt
     8821 aaagtaccat tatcattaac atctactttt atcatatttg aagaagcata aagaccacta
     8881 ttttctttta gagagctagg cattttaact tgttgtggct tactaaatgt tttatcaggt
     8941 tttatttctt taattttacc tttttctttt aatgcagata gtaagatttc ttgtccaatt
     9001 atttcgttaa gttgactact tccaccagaa gaaagaactg caactgccat attttcatct
     9061 ggaagaacta taagattact gtggaataat aaagagtctc caccttttgt aagagctttt
     9121 aaattatact gattaaatgg ataagtgttg acacaatccc accctaaacc atatccaagt
     9181 atagaatctt ctccttcagg ccatagacca tttaaatatt ctttgttttc catagctttt
     9241 actgaagcag gagaaagaat accatttgaa tttttcataa atgtttgtgc aaatgtacat
     9301 agattttctg cacttgaata taaaccacct gcacctatag cattaaggtt atcttgtgga
     9361 actgcatctt cccaataagg tacatatgct tttgctagtt ttgaactatc aaaactattt
     9421 tctgtagttt ttgtattttg taagttaagt ggattattta tgtatttatc aagaaaatta
     9481 gtaaatgaca taccagatac tctttctact aaaatttcag ctaaagtaaa tccatcatta
     9541 caataaacgg aaaaagctcc tggtttagct tttaatcttt gtttttgtaa ttcttttaag
     9601 aaattatcat gaccataaga atcattgtct gctaataaaa tagtgttttt aaaactactt
     9661 cccatcaacc ctgatgaatg atttaataac attcttggag tgatttcctt gtatctatca
     9721 tcagccattt taaattcagg tatatacttt acaacaggag tatctaaatt taattttcca
     9781 tcatcaacca acttcataac agctgttgta gtaaacattt tactgataga tgcgatacta
     9841 tacatattat ccttatttaa gtttttgtta tcctgtttgc tgtaaactcc accattacca
     9901 gaaatctcaa ttttgccatt atcaatcaaa gcatattgag cacttacagc accatacttt
     9961 gatagcaaag tttggatttt ctctttagca agagctttag tttttgtgta agattccgcg
    10021 tttttactat aatcatgtaa ttctatatca gaactttgat atttttcgga attatctttt
    10081 gtattatcag caaaagtaat atttggagca gcagtaaata acatactcaa tactaatgta
    10141 ctagataata atttagagat cctttgctta aacatattac attacctctt tcccttttta
    10201 ataatttata attagcataa cattcttaat tattttaatc aacagattat tatcaataaa
    10261 aaaagctttt ataagtatag ctttttatat aacagagaaa aatataaaaa taatatttgt
    10321 tgttgtataa agaataaagg gtataggtaa tatataaata aaatggagga attttagaca
    10381 tatgtataag aatataaata agaagcaact taaagaaatg atgaaaaatg aaaaggatgt
    10441 tttattatta gatgtaagaa ctaaagatga atttaaggaa tataaaatag aaaatgctat
    10501 aaatatatct ttacaagatt taatcaataa tatagatgaa atttacgatt ataaagataa
    10561 gaaagtagta gtttattgca gaagtggtca tagaagtgtt actgcatgta atttgttagc
    10621 tgaagaaggt tttgagcatt tatataattt aaattcagga ataattgatt atatgagtta
    10681 aataaggtaa gttatacatt aaatactatg tatagctctg tttattaagt aggaatatat
    10741 ctaaatattt ataagataaa tttaggaaag aacgaatttt ttcgcttttt tctatgacag
    10801 tttttgtagt aaatattagt ttttaagttg gaaaaaggta gtagcttttt agctactacc
    10861 ttttaatatg aacttagaat attattaaaa aaatatattt aaagacagaa ttaatttttt
    10921 aatgaccagt taaattacta tcgccaaatt tatcattaaa tggttttata actctattta
    10981 taggtccttt tagaagtacg tttaaacaaa tagacagaat cataaatagt aatagaacta
    11041 gtatatcttt agttagattt ggagcatata taccaccaac tgcttccctt gtagcaccta
    11101 ttgcataggt aaatggcatt atagggttta ttctttggaa gaatgtaggt gtaacttgta
    11161 tagggaatgt accaccagaa gaaccaatct gaacgactaa tagtactatt gcaattgctt
    11221 ttcctacatt tccaaataca gaaactaaag aatacactat aaagttaaat accatactag
    11281 taaacataag cagaactatc aataaaattg gatgttttgc tgttactcct aacaataata
    11341 tatctccagt tcctattata aaaccttgga caagagctat acttaaaaaa gtaagacctc
    11401 ttccaaagta aatttcataa ggtttatagt ttccatgtac agatgtagac aatagtgctg
    11461 aaataagtaa tataccaacc catgtagaaa gaacactata aaatggtgtc attgcagaac
    11521 cataattttc aattggatat aatttctctg ttttcacctc aacaggttct tttaagaaat
    11581 tagactttgt caatatatca ctttcaagca agctgactaa ttttttcata tcttcaccat
    11641 tactaatttt gcttaaagta tcaatcaaat catccaacat tccttttgct aaaggaagtc
    11701 tttctcttat caatgatata ctttcctgtg catttcctga aagttttaga gaagtagtaa
    11761 gtatttcttc aacctttgga agttttgctt ctgccttatc taaaacagta attatatcat
    11821 ttgctacttt tatactgttt gcaaatatac tatttatagg ttttgatatt tttgagtcaa
    11881 aattatttaa aatatttaaa ttaattcttc caataccatt tgatagactt aaaacattat
    11941 tcaaagcact tatagatggt tgttgtccag atataacttt gtttttaata tcatttaatg
    12001 ttgatataga tgaatctatt ttattgctag agtcttcaag attatctatt acatcatcca
    12061 atctattatt tgatgtaagt tggtttaatt tagttaaaaa gtccaccaaa gtatcgttta
    12121 aagattgcaa attagaaagt ttttctgata aattatctat caacttaggt acatcttcac
    12181 tgccactatt tactgcatca attaaattta atgtaagaga agaagcagaa gatgaaaggt
    12241 caaccattaa atttaagtca gacttaataa gaggagaaag ttcgtctaaa ttatcatttg
    12301 tatcctctaa gaattttttt aagtcacttg ataatagttt tgtatcattc agagtctttt
    12361 taattgtagg taaatcagat ttaatatctt taactgtatc agataatttt gaagtagcat
    12421 cagaagctaa atccacagtc ttatttatat ggtcaaatct cttttgaagg tcaataaggg
    12481 aattttcaac ttttgtcaat tttggaagac tgtgttcaat tcctgcacca actccgtttg
    12541 atataccaag aacgatttca ctaactgttt ttactatagt ctgattaacc tgtagttgta
    12601 ttgtagaagc acctttatct gtaatctttg gtgcaactgc atttattttt tcattaacag
    12661 tatagataat ttcgcctttt ttaacatcat ctgtaattaa agatgtcaaa tcttttgtaa
    12721 aattttttgg aatcttaagt gatgcataat aagtaccttt tttaacacct tcaagagcag
    12781 ttttttcatc tacaaactgc caacctaaac tcttgttatc tttgagttgt tcaattaatt
    12841 ggtctcctac attaatcttt tgtcctgcta tttcatttcc tttatcttca tttgttactg
    12901 ctacaagcat attagcagtg cttccataag ggtcccaaga tgcctttata ttgaaccaag
    12961 catacagaga tggtaaaaaa gcaagaccta aaactattac aagtacggca taattgccac
    13021 gtatatcttt taggtcactt ttaaaaattt ctaatatatt ttttaaaccc tttgaaggtc
    13081 ttaaattctt gatattattc cagtttaaaa tttcttttaa actattagtt tttttcaaat
    13141 gtctcacctc ctaaaatatt ttgataaaga aaaaagttag atttataatc aattttaaat
    13201 tatattactt tttacacaaa tagtaaagtt aaaattttgt ctta
//
LOCUS       DAEPOG010000111        13193 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEPOG010000111 DAEPOG010000000
VERSION     DAEPOG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.111"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(31..810)
                     /locus_tag="KR432_002539"
     CDS             complement(31..810)
                     /locus_tag="KR432_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641067.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(807..1286)
                     /locus_tag="KR432_002540"
     CDS             complement(807..1286)
                     /locus_tag="KR432_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0641068.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            2092..2793
                     /locus_tag="KR432_002541"
     CDS             2092..2793
                     /locus_tag="KR432_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0641069.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            2819..3475
                     /locus_tag="KR432_002542"
     CDS             2819..3475
                     /locus_tag="KR432_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641070.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            3682..5322
                     /locus_tag="KR432_002543"
     CDS             3682..5322
                     /locus_tag="KR432_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0641071.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            5681..7003
                     /locus_tag="KR432_002544"
     CDS             5681..7003
                     /locus_tag="KR432_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641072.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7200..8207
                     /locus_tag="KR432_002545"
     CDS             7200..8207
                     /locus_tag="KR432_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0641073.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            8245..9642
                     /locus_tag="KR432_002546"
     CDS             8245..9642
                     /locus_tag="KR432_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBH0641074.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            9787..11052
                     /locus_tag="KR432_002547"
     CDS             9787..11052
                     /locus_tag="KR432_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0641075.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11094..12133
                     /locus_tag="KR432_002548"
                     /pseudo
     CDS             11094..12133
                     /locus_tag="KR432_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            12168..13190
                     /locus_tag="KR432_002549"
     CDS             12168..13190
                     /locus_tag="KR432_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0641076.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4676 a         1544 c         2510 g         4463 t
ORIGIN      
        1 aactcataaa caacttatta ttacttatgt ttattttgca tctattactt catgagccat
       61 tttaactaag tcattcatag ataaatttga gtcttgaact gtaaaagaat agtaaactcc
      121 atcttgtttc catgtcaaaa acttaaattc attaacttca actttgtctg attcattatt
      181 tggccctaca ctaaatgtgt atttgcctga tttttcatca attttatcct gttctgtcat
      241 tttataacct tctggtacaa acttttgtgc gaatgcttca taagaaatat taatatcttt
      301 gtaagtatct acaactttta gattgtcaga actcttctca agtacattat ttgctgtgtc
      361 aagagatatt tcatcattgc catttttata ttcaaaacta agtgcttttg attttcctaa
      421 aacattattt tctttgtcaa gtgctttttt atttacagta tatgctcctt taaatttata
      481 tccattacta aattcagaaa ctatattagg ggtaaagtta aagtcttttt ttacttgttc
      541 tgtagttggg atagatgtat aggtagcctt agtacttgat tttccaacta aactatatat
      601 atgtcctact gcaagggctg ttgtgctcaa tatcaaagta atggctaaag ttgcaactaa
      661 gccttttcta aatggtaatt tgtgtgtttt attattaatc attttataat tttcctctct
      721 ttctggtaaa ctatttagtg ttttacttaa actattatga aaactttctg gaacttctgg
      781 aaacgaatta tttaatatat ttttattcat atgattacct ccgtgttttc taatttagtt
      841 tttaataatg ttcttcctct tgataatcta ctttttacag ttccttgagg tatatctaat
      901 atttccttaa cctcatctac tgaataacct tcaatatagt aaaggactat tggaatttta
      961 atttttttag gtagactttg aatagcagta tacaaatcgc tatagtcatc tttttcatca
     1021 atttttttgt ttttgaagac atcttcaaat gatagagatt ttaatcgttt gcgtcttaat
     1081 gaattacatt catttatgac tattctaatc agccatgttt taaagtattg ttcttcctta
     1141 agagaatcca acttttcata tgcttttaaa atagcattgt tgacggcatc ttcacagtct
     1201 tggtcgtttc ctagaataga cttagatact cgatacaaag tctgttctga ttcaagtata
     1261 ttgtttgtaa atgtagtttt atccaactga gttccttccc ttctatgcaa atcgtagcgc
     1321 tacttttttg cctttcactt attagataaa attttactat agatggttca ctttttttat
     1381 gattatttga taaatttaag tttgaaagtt ttgcctattc tatacaaata ttctataata
     1441 tatttttttc tagtattttc ccctcatata taaagccatg tagatatatt aaactttaga
     1501 agccatttat atatttagtt ttatagtaag tgtttatatt aagtaattat aaaatagtaa
     1561 gtatttgatg ttgagtagtt agaaaataat gcttttggat tatttacttc atgtatagat
     1621 ttaaaataag ttttttaatt aatgactaaa gataacggta ttttgactaa aagtaggctt
     1681 ttaagaaaaa acaatgaaac ttaaatgaga atcaagagtt agaaatgtca ttattttaga
     1741 tagatatgaa cataaataaa cttaaaaaat ataaattata aaaataataa tttttttaaa
     1801 ataacaaagt tttttgatta aaagttttat attgaaatgt caaagtaata aaatacctta
     1861 taagttaatt acaggttaaa aaaataatat gtgaattttc tgatatttac taattatcaa
     1921 taaagtattt aagtattaat gtttttagtg agactttaag acagataatc tcctattaaa
     1981 gatactatat cttagataaa ataaattaaa acaatataaa aacataacaa gattacagaa
     2041 agaaagctca taataaaaca ctaaaaaatt tattttaagg aggaataaag tatgaagttt
     2101 caagaaatga ttaatagccc aggtctttgg attgtttcta gttttttagt aattatttca
     2161 gttgttcaag caatcgtatt tatgaaatca gcacttaaag aggcttcaaa tcttggtatt
     2221 gaaagaaaag gaattaaagc agcaattcgc tcagctagtg ttacagcaat tggaccctct
     2281 ctatctcctg ttattacatt attatcactg gtagctgtga ttggtgctcc tacgacttgg
     2341 atgaggcttt gtgatgtagg agctgccaga acagaacttg gagtaatatc tcttacatca
     2401 aatttatctg gagtagaagt aggtagtgct gcatttggag cagaagcttt ttcgtatgca
     2461 ctatggggaa tggctttaaa taacttaggt tggttgcttg ttgtatttat tttgggtcat
     2521 agaatgcgtg gaattgttga aaaaatgaat gtgaaatata atcctacttg ggttaaaaaa
     2581 ctacttgcag gggctacagt tggattattt gcttaccttc tttcaaatca aataaaaaca
     2641 ttcgaaatac caaaacttac accagcagtt atttcagcta tagttatgct agtattgacg
     2701 actttattta aaaagaatca acgattacaa gaattatcct taggtattgc catgttgatt
     2761 ggtatgtttg gcacacagat tgtactatca tgattaaaac agataagagg tgataattat
     2821 gtatgataaa aagatgataa aaatagggag aatttctata ggtcttgcaa ttattgcaaa
     2881 ttttattcct gcaatctatg taggtcttcg ctttggtcag atgccaatgc tttctgttat
     2941 attccaaatt tggggattag ttgctacaac ttatggagtg agttggattg ttcaacctat
     3001 agcttattat ccaacactag gagctccagg ttcttatata ggttggcttg caggttctgt
     3061 tggagatata cgtatgcctg cagcatcaat ggcacagaaa attgcaggtg tagaagcagg
     3121 aactcatgag ggtgaagttg taggaacaat tggtacatgt tgttcaatct ttgtatcagc
     3181 ttctatgatt actattttta ccattgtagg ctataaagta attcctttgt tacctgaatt
     3241 tattactgat tcatttaatt atatacttcc agcattgttt ggggcaattt acgttgatat
     3301 tgcgcgtaaa gatattcgag ctggtggatg tacgattgct gtagcattag ctattatgta
     3361 ctttggtgaa aaaattggta taccaggtgg attgttgaca ttgtttattg tgttaggtgg
     3421 gatacttatt acaagggttt tctttgtaat tgatagcaat aaaacgaaag aataggattg
     3481 tttactagga agaaagacta gaattatttg ctgagaaaaa gaataggatt gtttactagg
     3541 aggaaagact agaattattt gctgagaaaa agaataggat tgtttactag gaggaaagac
     3601 tagaattatt tgttgagaaa aaagaatagg attgtttatt aagaaatatg aatataaata
     3661 agtcttgaaa ggagaagaag aatgtttgat gttaaaattg ccaatggaat aatcattgat
     3721 ggtacaggaa attcaagata taaaggtgat gtagggattg taggagacaa aatcatggct
     3781 attggagatc taagtcaaaa agaggctaaa gagactatag acgcaactgg aaaatttgta
     3841 tcacctggtt ttatagattt ccacactcat tctgatttat ctcttgtgta tgacaaatac
     3901 acaagaagcc gtatccatac aggtgtaaca acagacgtta ttgcaaactg tggtatagga
     3961 gttgctccga taagagaaga gaagaagcag gagttaattg actatcttgg aacgaggata
     4021 attggtacaa ttccaataaa attgaacctt cactggaata caatgcagga atactttgat
     4081 tacttaactg aaaattctcc agcagtaaat gttgtagctt atgtagcaca aggacctgta
     4141 cgtatcaatg aaatgggatt ttcaaaggag ccagctactt tagagcaact taaaaatatg
     4201 aagttggaag ttagaaaggc tatggaagct ggatgtgtag ctctttcgtc aggattagtt
     4261 tatttaccag gagcatatac aaagaaagaa gaaatggctg aattatgtaa ggaattgata
     4321 ccatacaatg gatactatat atctcatatt agagatgagg gtgatgaaga aatggaagct
     4381 cttgatgaag caatttatat agctaagacg gcaggagttc ctctacatat ttctcattta
     4441 aaagtcatgg gtcataagaa ttttggaact attgataagg tatttaaaaa gctagatgaa
     4501 gctgaggcag atggcttgga agttactttt gattgttacc catatacagc tggaatgaca
     4561 tctttgggtg cacttttacc tccttgggct tttgaaggtg gtgtagaaaa catggtaaaa
     4621 cgattagaag tgcaagaaaa tagggacaga attataaaag agctagagga agggattcct
     4681 ggatggcaat gtttctacca gttagcaggt ggttgggacg gtgttgtact agcctctgta
     4741 atgactgaag caaataaata tgtagagggt aaaactttaa tggaggtagc taaaatcaat
     4801 ggtgaaaatc catttgatac gttctttagg cttttgattg aagaaaaaag taaaattcaa
     4861 gtagttgttc atacacaagg tcaagaggat acagataagg tcgtttgcca tccaaagtca
     4921 tgtataggtt ctgatagtat ggacctttct acagaaggct tactttctct tggaaagcct
     4981 catccccgtg catttggtac ttttggacga attttctctt actatgttag agaaaaaggt
     5041 atgttgactt ttgaggaagc agttaaaaag ataacttatc tttcagcaaa acgcttagga
     5101 atttataaag aaaggggtct acttaaggaa aattattttg ctgacattgt ggtatttgac
     5161 ccagatacaa tagaagataa agcgacttat agtaatccta aacagtatac agtgggagtg
     5221 gaatatgtat tggtaaatgg taaaattgca ttggcaggag gaaagcaaac tgatgtatgt
     5281 gctggacgtg taattaaaaa tcctcttagc atagcaaaat agaatacatt aagaactttt
     5341 ataaaaatag tctttctttg aatattaagg aaggctattt ttgttaaatt aataaagatt
     5401 actctacttg tgttttctag agaatttaac tttaagtaga aaaaacaata tattaattgt
     5461 aaaatgtaat taatattaat ggataagatt taaatacaaa aacttaaata caaaaatgtt
     5521 ttaacagata gtcataatta aaaaacttaa agtaactttt tatataagat ataattgatt
     5581 atgcaaaatt ttatgaagca gcaaccttgt tactttaagt caagggaggt tcagaaagtg
     5641 tcttaagaga taatagtatc tttaggaaag gagaaacgaa atgaaaatac tttatgtagg
     5701 accaggcatg gtaatcgaca aaatactagc agtaagtaaa aagaattttc ccaatattga
     5761 agcagatttt cttagatatg atagatatat tgaagtacct gatttattgg aaaaatatag
     5821 aggtagagta gatgcagttc tttttacagg taaatcgcca tttaaatttt ttgaaaaaag
     5881 acaaaaagat tttgtgttgt ctgattatat tcctagacat gaaacaacat tatacagagt
     5941 attgcttgaa attacatatt tgcttaaatt cgatattagt agacttagta tagatactta
     6001 tgataaagct atgctgaaga gattgtataa agaaattgga gttgaattta aggatgaaca
     6061 tatgtttttt gcagagcaaa agtatttaga agaggactac ataaagtttg tgcttgaatt
     6121 tcataaatat aattatcaaa ataatgacat atgttgttgt attacaggtc ttgaagaagc
     6181 atatcatgca atgaaaaaag agaatattcc agtagtttta gccttgcctt ctgaggatgt
     6241 aatggtacaa agtataaaaa acattcagat gcgttatatt gcaaagaaaa acaatgagaa
     6301 tcagattgtt gttcttgcta tcaaacttaa tatgccttca gaatattcac tgatgaagga
     6361 agatgagtac gcttatcttt cacaaaggat taagattctt gataaactat accatttcaa
     6421 tagccgtata gatggtgttt tggtagagca gagtagaagc gaatttatga tttttacaac
     6481 taaaaaaata cttgagttag aaacaaagaa gtacagagat atttatttgt tggatatgat
     6541 tcgtgaagtt tcagttataa acacttatat aggaattggt tatggaaaaa cagcaaatga
     6601 agctaaattt aacgcttatg agagtattaa gatggctcaa agtcaaaaaa atgatgcagc
     6661 atatgtagtt tttgaaaatg gagaaattat gggaccacta aagccaaatc ctgctgtcaa
     6721 agagaaaaat agctttgatg aacgatttta tcaaattgct acagagacag ggctaagtgc
     6781 taatactgta tataaagtgt tcaataaaat aataaatagt ggaaaaactg aatttacatc
     6841 aaaggaatta gctattatat gtggtgtaag tattagaact atggacagga ttatacttag
     6901 attgtgtgat tctggttatt gtgaggtagt gagtgaacag cttatgagca agtatggtag
     6961 acccagtaga atattacgtt ttaaaccttt tctgttgttt taaatagata taagttaaaa
     7021 attaaatttt tatgataaca ttttctttac aattttttag atatatgtta atatttagaa
     7081 aaatgcatat gtattcataa aataaactca aaatagctaa aaaatcatta aaaaggaaat
     7141 cgtatgcata aaatgagaaa gttaatttat aacttaaata aatctgggag gagataatta
     7201 tgggaaaata tcaggattca aaaaaagttg taagaaatta ttttgaagca ttagaaaatg
     7261 ctactgggga tgaggttgaa aatgtcttaa agcaacatat gaaatcagat tataactgga
     7321 aaggtgtgta tccatttcgt gaacaagaag gcactgaaaa tgtagcagat gtattttgga
     7381 agcctttaaa aaaatcattg agtaatatgc aaagacgaca agatattttc attgcaggaa
     7441 caaatgagat tgacaatagt gaatgggtaa tgagtatggg gcattttatg ggactttttg
     7501 ataaggattg gcttggcatt aaacatactc gtaaaatgat tagtttaaga tatgcagaat
     7561 ttaactgtgt tcaagatggt aaaattgcta aaactggatt atttgtagat ataattgggt
     7621 ttatgattca agcaggagtt aatccattgc caccacaaac aggttcatat tttgtatatc
     7681 cagggccaat tgaccataat ggattacttt ttgaagatgc agatgaagtt gaaggagtaa
     7741 aaactttagc attagtaaat aaaatggtag atgatttatc tgaattaaat gaaagtggta
     7801 ctatgggatg cccaccagaa atacttaaaa aatcttgggc taaagatatg atttggtatg
     7861 gtccaggtgg aattggtgct tcttatacta tacctagata tcaagagcaa caccagctac
     7921 catttagaaa taatttaaaa ggaaagactt tcaatgggca tgtatgtaga tttgcagaag
     7981 gtaattttgc ttgtttcttt ggatggccta atctgactaa tgtaccatgt ggaggttttt
     8041 taggattacc aggtggagag gttaaagcag atatgcaagt tgttgatgtt tattatcgta
     8101 agggcgataa acttcaagaa aactgggtgt taattgatat tccttattgg ttgaaacaac
     8161 aagggcttga tattcttgag agaactaaaa gtatttttaa tgcttagtta tgcatacgta
     8221 ttctaaaaca aagggggata taaagtggca tctaccaaca aaaataatgt aacaagtaaa
     8281 gaggaagaaa aagaagtttc tacaagtggg cttgaatcat gtacggttaa agaaaacaaa
     8341 gctaaaatga atcaaactaa ttttagtaat ataaatgaaa atcaaacaaa tgagaataaa
     8401 acgaacacat ataaaggaaa tatagataaa ataaacacaa ataaatccga cataaataaa
     8461 gttaacatag atacagtgga tacagataaa ttaaatacaa atactcttac tgaaaaaaat
     8521 aaaaaagtta tatttaaggg ggaagataat atgtcatcta caaaaattta ctatggagat
     8581 tcattagaag taacgcctgt tggagtaatg gattacaatg atttttcaaa acagacaaaa
     8641 agggaacagg aaatacctgg atttgattct aagtatagag attttgtaga ttatattatg
     8701 aagattacac ataatatctg ggaagaaaaa ggtataggtg ttatttatga tacatatcac
     8761 aataatgtta caatgcattg tggctcttca aatttagttg gtattaaaga tgttatttcc
     8821 aatactcttc aaacattaca tgcgtttcct gatagaagat taattggtca aaatgtaata
     8881 tggtctaact ttggtgctga tggtttttta tcttctcacc gtgttttgtc tacagcaact
     8941 aacttaggtg atagtaattt tggaccagca acaggtagaa aaataaactt tagaactgta
     9001 attgattgtg ccacaactaa caatagaatc cacgaagaat ggttggttag agataattta
     9061 tggattgtta ctcaattagg attaaaccct caggaagtag caaagagtat ggcaaaggca
     9121 agtgaatcta aagtgcttag cttacaatca acttatggta tctgtgaatc tatggatggt
     9181 caatttatgc caactaagta tcaagcaaaa gatgattctg ttggcgaaat gatgcttgaa
     9241 atgacaagcc gtatttataa ttataaatat ataaatgaag ttaaaaaata ctatcatgat
     9301 aatgcagtag tacattttat atgtgataaa gatttaaatg gttatgatga gattcaagga
     9361 atgattgtaa gtttacttgc atcaattcca aatggaagtt atgaagtgga aagagtaact
     9421 tgtaatcaaa gagaaaaaaa tgaaggttat gatgtatcta taagatggag attacgtgga
     9481 ataaatgaag gtataggatt ttttggacag ccttctggaa agcatataga agtaatggga
     9541 atcaatcatt atcatatact tcaaggtaaa gtaaaggaag agtggataac ttttgatggt
     9601 atggatgtat taaaacaaat gtatatggga gtagaagaat aaagctgttt agagtataaa
     9661 taattttgaa atgtatatag ggaagtattg ctttataaaa tttaagtggg gaatgtatat
     9721 gaaaaataga tgtttaaact tttagcaaaa attatgacta aatttaaaag ggggaattat
     9781 aaaaatatga aaacaacggt tgaaatagaa aatgatatca tattaggaaa agactattca
     9841 aacctaaaaa gaggaattgc atttttttca attgctttaa tatacttttt ttattgctac
     9901 aattttatgg tgggtacttt tattaaacca acaatgattt atgctttagc agatggggga
     9961 tttggctttt cattaaaaca aacagaagaa atatttgcag ttatgtcttt tggtacaatt
    10021 ccaggtacat ttatttttgg ggtgatttct acaaaaattg ggaagaagag gactttaatt
    10081 agcgttgctt tactgattgg attgacaaca tttataccaa tgttatctcc tacagatatt
    10141 atgttatgga aaatagcacg tttgtgtaca ggagtagtgt tgggaggcgt atttggaaca
    10201 gctatgcctt tagttgcaga tatgtttcca agtaaatatc gtggtaagtt agctgctata
    10261 ttaactgctt tattttcatt agcaatgatt tttggtggga aagtatatgg cgttttaggt
    10321 gatgcaaatt ggcaaatatt gatgtatact gcgattatac cacctatagt tggagctatc
    10381 ttagcaatct tctttgtgcc agatgatttg gagtatacaa aggaattagt taaaaaggga
    10441 aaagaaactg gtgaaaagat tagctatata agtatgtata agggaaaata cttatggatt
    10501 ggattgggaa cgattctttt atcaggtgca aattttacag catattcagc attttcaaat
    10561 aatgcaacta catacctagt gacaggaata ggaatgtcag cagcagtggc tgggtctata
    10621 tatagtttac aaggaattgg tcaattagta ggatatttat tctggggttc aattgcagat
    10681 aaatttggaa gaaaaatacc ttttataggt atggcattag cagcagtatt tgtttttata
    10741 tatacaaaat taggtggaga taatacaaca acattctata tgatatcagt attactaggt
    10801 gttgctgttg gatattctgg agcatgggga gcatactaca cagagttgtt tccaagtaaa
    10861 tatcgttcgt tgtcatctgg tatgtcattt aatggaggaa ggattatatc tacgtttgca
    10921 atacctgcta tagctggtac agcttctggt actttaggta tgatgactat cttttacata
    10981 tcaatcgcag tctttgtagc aggtgctatt gtatggttat tcttaccaga gacgttaaat
    11041 aataaaaaat aaaatactag gaatttattt tgttacttag aaaggggtgg cttatgattt
    11101 catcaattga tgtatgtatt ataataggat atttgctatt aatgttagtt attggttatt
    11161 attcaggaaa agataataaa aatcaagaag attatttctt ggcaggtagg tcaatgccat
    11221 ggattccaat tgctttatca gttgcagcta ctatgattag tgcaaatggc tttataggtg
    11281 gtcctggatg ggcatatgta gatggaatgt atccagtaat gtaaaatgtg caaaaaattc
    11341 ttttgtagtt agtgcatttg ttatgaattt agtttactac tttatgctat tggtaggagt
    11401 aattttattt gttttttatg ggggaaggac atttgaaact tcaaatgaaa taatgattac
    11461 ttttatttta aatgaacttc cagtaggagc cattggaatt attattgcag gtgtgtttgc
    11521 agcagcaatg tctagtatcg actctatatt aaacagtatg acaactgttt ttacgaagga
    11581 tatttatgag ttttatttca aaaaagacaa caaagaatca tcattaaaag ttacaatgat
    11641 tataactgta gttataggag ttgttatgac aggttttatt attatgggat ttggaggaag
    11701 catcaaatca attcttgacc ttgttggtaa ctatatctca tactttgctg gaccagctac
    11761 aggtgcattt gtactagcaa tgtttacata taaagctaat gataaaggag tatctattgg
    11821 ttttattgta gggttagttt taggattttt tatttctaat aaatataatg tgagttggtt
    11881 gtggaatcca gcaataggtg ctacaataac attgatattt ggatatattt ttagtgtcat
    11941 tttaaagaaa aatcataaag tagatgatat taaaaaatac actgcatttg gtatgagaaa
    12001 tcaccttatt tctcaaggaa tggataaagt agatggagta tctatagtgc cattttcttt
    12061 aggtaaacaa gaactaattg tattagcttt ttttattatc caatatatta tattattttt
    12121 gatacaattt taagaagaaa tactagatat ttaaggagaa atactaaatg aatcaaaaga
    12181 aagttcatta taataaatcg gctacatcaa aagatgtcgc aagattggca ggagtatctc
    12241 aatcatctgt ttcaagagca tttggtagtg caaatggaaa gggcgtaaaa cctgaagtaa
    12301 gagaaagaat ttttcgtgta gcaaacgaaa tagggtatgt tcctaatttg gtagcacggg
    12361 gaatgattag tggaaaaact aatgttatag gtttgatagt tggtgatagt ctaggtccat
    12421 tttacaatag aattatcaat ttatttgtag aaaaaattca agagataggt aaacagtgtc
    12481 tcgtttttaa agttcctaga caacaaagta tagatagtat aatttcaaaa gtaattcagt
    12541 ttcaagtaga agctgtgatt attacagctt cggccatgaa taaggtgatg gctgagactt
    12601 gtgaagaaaa caatattcct gtgattcttt ttaatcgttt tatacctggt ataaaaataa
    12661 gtacagtcta tgtagaccca attgaaggtg ggggcatggt tgctgaatat ctgcttaaga
    12721 aaggtcataa gaatatagga tatattcaat ttacgagaga aacaagtgaa gaaatggaaa
    12781 agaaaattgg attttattct aagttaagac aaagtgggat tcataacctt aaagaagaat
    12841 ctgcaaacta tgattatgat tctggatatg aagcaggtaa gagaatgtta gcattatcaa
    12901 atcctccaac agcaattttt tgcacaagtg acttgatagc tattggtgta atggatgctg
    12961 caaggtttga gtacaagttg agggtgccag aggatttatc agtgatagga tatgatgata
    13021 ttcaaatggc ttcatggaag agttataatt taacgacgat tagacaacca ctagatttgt
    13081 tgattgaaaa aacaattaac atacttaagt ccttattaaa tgaagaaaat ccagagtcag
    13141 tcgttgaaat gttaaaacct gaattgattg aaagaggttc tacactttaa gtg
//
LOCUS       DAEPOG010000112        12992 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEPOG010000112 DAEPOG010000000
VERSION     DAEPOG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.112"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(168..977)
                     /locus_tag="KR432_002550"
     CDS             complement(168..977)
                     /locus_tag="KR432_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0641077.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1061..1594)
                     /locus_tag="KR432_002551"
     CDS             complement(1061..1594)
                     /locus_tag="KR432_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0641078.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1721..3796)
                     /locus_tag="KR432_002552"
     CDS             complement(1721..3796)
                     /locus_tag="KR432_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641079.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(3799..4545)
                     /locus_tag="KR432_002553"
     CDS             complement(3799..4545)
                     /locus_tag="KR432_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641080.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(4559..4954)
                     /locus_tag="KR432_002554"
     CDS             complement(4559..4954)
                     /locus_tag="KR432_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBH0641081.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(5067..6089)
                     /locus_tag="KR432_002555"
     CDS             complement(5067..6089)
                     /locus_tag="KR432_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641082.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(6086..6757)
                     /locus_tag="KR432_002556"
     CDS             complement(6086..6757)
                     /locus_tag="KR432_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641083.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(6843..8192)
                     /locus_tag="KR432_002557"
     CDS             complement(6843..8192)
                     /locus_tag="KR432_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0641084.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(8204..9586)
                     /locus_tag="KR432_002558"
     CDS             complement(8204..9586)
                     /locus_tag="KR432_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0641085.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            9830..11044
                     /locus_tag="KR432_002559"
     CDS             9830..11044
                     /locus_tag="KR432_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0641086.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            11221..>12992
                     /locus_tag="KR432_002560"
     CDS             11221..>12992
                     /locus_tag="KR432_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0641087.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSN"
BASE COUNT         4649 a         2118 c         1624 g         4601 t
ORIGIN      
        1 tgacaaggaa taaattataa caaattttag gaatatataa taatagggtc tcagaactta
       61 gttacaagtt ctaaaaccct atttgtttag tgtaaaaaac atcataaaat ttagcttaaa
      121 tcttttcaca taatattact ctttttctaa gtgataataa aatatatcta ttcctctaaa
      181 aactgtaaaa attcctgatt aaatagttta agctcatcat agaatatagc atgaccacta
      241 tactcaaaag tatataaaat tgaatcttct attttttcat tcataaaaac tgcaaattca
      301 tacggacata tttcatctaa tttcccatga aatataccag taggcactct tacacatttt
      361 aaatcattaa ataattcttc atctcttaaa gatacagcag ttccaatagt gccaatacct
      421 gatgcactaa aaccaatatc attaaaccat cttctaaagc tttctggtgg atttgatgca
      481 aatacttttt ctccaaaatc ggttaccatt tctggtctat cagtacatgc ttgtgctatc
      541 aatttattta catcttcacg tgtcatacca tatggaaatt ctggaggtct ttgtacaaat
      601 gatggtgcag cagctgcagc taataccaat tttgacactc catatccatt aaataaactc
      661 atatacctaa gtacgattgc tcctcccatt gaaaaaccaa caaaggtaaa gtcttttaat
      721 ccaatagcat gtaccacctt ataaatatcg tctgctaatt ggctataagt atatcctcct
      781 gatgttgcat cagatttacc aaaacctctt aaatcaattg aaactgttct atatccgagt
      841 tttggcaata tatttgtttg atattcaaat atcttatgtc ctaagggcca accatgtata
      901 aaaagtacag tttttttagc actcggattc aaatcatata ctgctatgtt tatatcattc
      961 acatatacat aatacatatc aacagcaact cccttcaaga aaatgtatga tggttatttt
     1021 atatatatga ataaaatgat gaataactaa atttttaaat ttatttacaa tacttctcta
     1081 ttgccttttc aaataaactt acatgaagtt tttcatctag gattattcta tttaatatag
     1141 cttttatata agggtcttca ataagactta tatctttata ataattatta atagccacat
     1201 gctccaaatg caaatctagt tttaatctgt tacaaataga atttccataa catacaaaac
     1261 caccattcca atatgcacca caagaacttg tagaacctct atatacagga tttccaccca
     1321 ataatcttat agtctttgct aatatctcca tatgtaacat ttctgttata gatatattta
     1381 tccacatttc acttaactct ctatgcgttt catctaaatc aaaattatgg tatagatatt
     1441 gatttacact cgtaaattca cttgatactc cggcatagtc atccatcaat aattctgcat
     1501 aatacttatt tagacctaaa acttttatct ctggatatgg ctcatcactt gaatatccat
     1561 ttcgcttgtg catgtcgtaa tgtgcatcac tcataatatc tcctccttta aatttaatac
     1621 ttgatattaa ttgtatgcat cacgactttt tcatataact tagataataa aatctattat
     1681 attattttct attataattt atgtgtcaat tcacccaaat ttatttgata tgcaatattt
     1741 ctttccttag tgctacatat gtaataaaaa catatataca atatattact acaaaattaa
     1801 taagcgtaat ggctacagca tgtaagatag gaatctgaat attaaaaaat actggagcat
     1861 caaaagaaat attaaacatg ttgtatgaaa aaactgatac caatattcca ccaaatatta
     1921 ctgcaggaat taatggaatc gaaaaatata aacaaacatg tttctttaag atttttatta
     1981 cttcactatc aatcatcccc aaagttcgaa gcattccata gtgttttatg ataatgttat
     2041 tttcgctcaa taattgaata gagagaatga ctgtaccaat aatgcatata atgagtgcca
     2101 aataaaaaag tggtaatatc aagataatgg cttgaccagt agattgcata gcatactttc
     2161 cacttatata atccttatta tcatatcgta atttagaaac aaagccttga ttacctgcta
     2221 ctactactcc agaattaaga ttttctaaag tatcaaactt tttctgaaac tcagtaaaat
     2281 aagcactatc caaaggctca cttgtaatag caacataaag actatacaga ggtttcatat
     2341 tatcaattaa ataatctgga atgacaataa tatattcttg tccatttcca tatcctccat
     2401 actgtgaaaa gttctctata taaatacctg caatacttaa gtccttatct gatattgtca
     2461 gtttatattt ttctccaata taattcttat atgcatcagc aagataaggc atacaatgga
     2521 tgataaattg atttttgtcc atctttattg ggtttaaacc caacatattt cttagtatat
     2581 tgtaatcact atatttcata aatgtgtcaa actggttttc tgcaaaagta taatccttat
     2641 attgtaaatc tcgccctata tctttcaagt atttagaaag gatattatta cgaaccttca
     2701 taaaggatgt tttttcttct gtgtaaagag tataagaata atcctttttc acacctttcg
     2761 tttctgataa atatccttca tatgaagaaa aatcatatga ttcattcggg tgaaaaatac
     2821 tcacatcaaa ttgttgtaaa tccactgatt tattcatcac agaataaaat gagttagcta
     2881 ttccaataca agcaattgat actgtaaaaa gagttgcgat aatacccatt ataatgctta
     2941 ttccatttat ctttgaagcc agtagacgat acaaaaataa gtgagatttc ttatatttcc
     3001 aattcatccc tttttcaaac atattctgtg caatagatcc aaatcccata aataatacga
     3061 ttagacaaaa tacaatcaac aataaaccaa gaaatacatc tgttaatcta ccactaggta
     3121 ttgaaaagaa aaaaataccc atacctccac acaaaacaga cacacaaaac ataatagact
     3181 tcgtttttgt ttggtgaatt aatacatttt cattatatcg atcataatta agcaaattaa
     3241 ttaatttcat tttaacaagt tttttattac tttttaattg agcaattaaa tatattatgc
     3301 agaagtataa aaaagtcatc ccagcagctt ttaatgaaaa atccggagat aaagaatact
     3361 ttccatgaaa catattaata acaatagatt ccaaaacctt agaaacaaat atccctagaa
     3421 tccatccaac aataagagca gaagcaccta ataaaagatt ctctataaaa agcattttaa
     3481 caatttgttt tttatctact cctaatatca tataagtact tatttctctg cttctttttt
     3541 tcaatataaa attcatcata taactaacaa accagcctaa aataaaagta ataatgaacg
     3601 aaaatatgat aatcatataa gaaaactcat cgcttcctcc attattaagt atactagaaa
     3661 gttcttgcat tacgtcggaa aatatcaaag cgttaaaagc ataaatcaaa gctacagagc
     3721 ctattaagga acaccaataa ataaggtact ttcctgcttg tcgtcttgca ttacgaaaag
     3781 ataatccttt caacataatc aatctcctcc catcaaagaa agtgcatcca aaatttcgtg
     3841 atagaaagta agtctggatt tttctccttt tctaagttcg ttaaaaattt tcccatccct
     3901 tagaaataaa attctctcag tataactagc tgaaatagcg tcgtgtgtga caagaagaat
     3961 cgtagcctct aatatggtgt ttatagtttc caaaagttcc attaaagttt tagatgaatt
     4021 agaatccaat gcaccagttg gttcatctgc caaaataagt ttgggctctg ttacaactgc
     4081 acgagctact gcacaacgtt gcctctgacc accagatact tcataaggaa acttcgaaag
     4141 tatctcttca attttcaatt gtgtggcaat attatacaca ctattagtaa tttcaacagg
     4201 agaaactttt tttattgtaa gtggaagtgc tatattttct tctaatgtca gagtatctaa
     4261 taaattagaa tcctgaaaaa caaaacctaa attatcacga cgaaaaacag ttaagttttc
     4321 ttcggatagt tcagtaatat cttgattttc aagataaatt tttccagcac ttactgtatc
     4381 aattgtagca atacaattta acaaagtagt tttaccagag ccagaagttc ccataatact
     4441 gacaaactct cctgtattaa tttgaaatga cacatcattc aaggcctttg taagtatttt
     4501 ctgattgcca taatatttct caatgttctc tactcgtaat agcatgttaa agtcctcctt
     4561 agtcaacaat ttttccattt ttaattatta ttgtcttatc tgaaaaattc aagatatata
     4621 catatatgct ttctttagag tcttgaattt gaatagtagg ttcattagat gaaattagag
     4681 atttttttac ttcaatatcg tattttattg ttaattcatt atcatctcca tttttattag
     4741 tatcttctac atttttattt aaatcaatgt tattatgatt aaagggacca gtaaacttta
     4801 cttctgtaat agttccattt tttctaataa aagaagcata ttcagacctt agtggtatga
     4861 tacttcgtga gaaaaaaccc atcgaatcca atcctgtaat tgaaataaac aaaacgagta
     4921 caacacataa tatagttaat atcctttttt tcatatgttt ctccctcctt tctattgata
     4981 ttgtagcgta aatagtaatc tgaatatata gaagttactt aactttatct tacagttttg
     5041 aaagataaag aaatagcagc gcatttttag ttgcactgct tctgcatatt gatagctgta
     5101 tttattggga aattcaatgt tattgctgtg tattcaccaa ggctggagga cacatctata
     5161 tcaattccca ttttcttaga cagttcataa caaaggtaaa gaccaatccc tgtagaattg
     5221 tttgtagtcc ttccattact gccagtaaaa cctaattcaa aaattcgata aatttcactt
     5281 tctttgatgc caataccatt atcttttata gtaaacttaa cactcttgtc ttcttttatt
     5341 gcaaaaaatg aaatttcagc tgaatcactt ttgtactgaa cactattgat aataatttga
     5401 tttaaaataa aaactatcca ctttttatca gtatatacac tatactccaa atcctgtata
     5461 ttaatatgaa tattattttg tataagaagc aatttattct ttgctaatac ttcttctaca
     5521 attgacttta atgttatttc agaaattaaa taatcttttt caacatggcc aaggcgagca
     5581 tagtacaatg cacgctcaat atgacgttct atttcttcca tttgaaaacg aatttttcgt
     5641 gtatgttcat ttttataatt ttcacatata agttctgatg ttgtaacagg atgttttatc
     5701 tcatgtaccc attgttcaat gaattgtcta tattcgtaca tttcacgatg tatttgagaa
     5761 acatgatctg tcatagattt caatgataat ctaataatat tgtagtattc acgttctatg
     5821 ttagaagtag gtttttcaat catttcaata aacagatatt ttttatccaa ttcgtctaca
     5881 atgcgacgta acttaataat ttcttttttc tgtttttgat aacaaaaaca tatccaacaa
     5941 aataaaattg cgacccaagc ggctataaga agagtcaact ctccttgacc aatccctata
     6001 agatgaagat atagcactaa tgaaattgca caacaaatat ttaagagtat aactaatact
     6061 ttatcttgaa gaaaattaca aaacctcata tcctatatcc catcccccgt tttgtaatga
     6121 ttaagtcagt aatacctata ccccttaact tttctctaag ccttgtaatg tttacactta
     6181 aagcattatc atcaatatga atatgattgt cccataaata ttctaccaaa tctatacgag
     6241 gcacaatact ccctacattc tgaaacaaat aatacatgat ttttagttca gttcttgaaa
     6301 gttctataac agcattttgg tattctactg tagctgaaat aaggttgagt ataatctctc
     6361 tatatttaat tttctgttta tctgatgaat ctactctttt aagtaggttc tttacccttg
     6421 ctaaaagcaa aggtaagtta tatggttttg taatatagtc atctcccccc atagtcaaac
     6481 catttagttc atcaattgca ctatctcttg ctgtaataaa aataataggt atagtagaac
     6541 aattcctaat ctgtctacat aaagaaaaac catctatatt aggcaaattg atatccaaga
     6601 gaattaagtc aggtgttgat tcttggatta cttgaagtac atcacaatta tttgtatcca
     6661 atgtttgtgt actatagcca ttattttcaa agaattgcat taattcctta cagatgaaca
     6721 catcatcttc aattatcaaa atattggtta aaataataaa tacctcattt ctttagttta
     6781 tattgcttta attttgtaag taatatattt ttccatatat tacttacaaa attacttttt
     6841 aattattcat cttttccagc tactgatagc tctttaataa caactgaagg tgaacctata
     6901 ctactcattg gaaattctaa atcttcccct ataacttcaa tatctttcaa caggtcaaaa
     6961 tagtttcctg ctactgtaat ttgctccact ggaaatactt tttttccttc tttgatataa
     7021 aagcctttgg cagctaaaga aaagtctcct gttactgaat ttgcacctga atgaagccct
     7081 gcaaagctag taaccatcaa tccttcatct atatccttta taatttcatc taaagattta
     7141 tctcctttat ctatataaaa attagttgga tatatgctta taggtgatga atatgaagat
     7201 tttattccat ttcctgtagt ctttacacct gccttattag ccgtttttaa gttatgaagt
     7261 aaagttataa gctttccatt taaaacaact tcttttttaa atgtagctac tccttcatca
     7321 tcaaaaggag tagatgccat accattttct aaaagtgggt catcaactat tgtaactata
     7381 ggagatgcta tcatgtctcc ttctctattt ttcaataaag ataatccttt ttgagctgaa
     7441 tctgcattaa atatcccaga aaatgtgcta agcaaagata ccatagcttc attaagcaat
     7501 atagttttat atttacctga tggaatactc ttaccaccta ctttagataa agcattatca
     7561 acaccaagtt ttgatatctc acaaggtttt atttcttcaa tagaatttgc tctattatat
     7621 cctattccat catatttttg tccattgtct tctatgatag gaactacaaa ccctataagc
     7681 atattagttt tattgcttaa attaagacct ttagtattag atataccatt acttgataca
     7741 gaataagata tagtacattt agataaattt accaccttat ctgaataagc tttagtctct
     7801 ctttccattt ctaaagctaa atcaatcaac ttatctgcct ctaaattttc tagctcttta
     7861 gaataagtct ttacatcatt atagtgctta tctccttcat atataaattg tacatcttca
     7921 ttttcaatag tgtttactcc atcttttaca ttttttatca acatatcaac agctttatca
     7981 tctaaaattt ctgtataaga ataacctatc tttccattta ccttacctct aaaagataaa
     8041 ccaaaagatt tatcaagatt gtatttctca acttctcctt catatatatt tatacttaaa
     8101 ttttctcctg tataataata tatttcacac tcttcaaaac cttcacttag agccttttta
     8161 aataatatat ctttaaaatt tttaagttcc acaattatcc ctcctatctt ccacctacag
     8221 taatttcttt tacccttatc ataggttggc ctacattagt tggtatactt cctgatgatg
     8281 agccacacat cccctgagct tgttttacgt tattcccaac catatctata ttcataagta
     8341 tatcgctacc ttttccaatt aaactagcac ctctcactgg ctcttgtatt tctccatttt
     8401 ttattaagta accttctgaa actgcaaaat taaactctcc tgttacagga tttacagaac
     8461 caccacccat ttttttagca taaagtccat ctggtattga ctttataatg tcttctggtc
     8521 tatcttctcc agcagcaata taagtgttag tcatccttga agttggtgca aatttgtagt
     8581 tctgtctacg agaacttcct gtaggattca ttcccattcg tctaccatta agtttatcta
     8641 tcatataaga cttcaaaatt ccattttcaa tcagtatatt cttttgagtt ggatttcctt
     8701 catcatctat attcatagaa ccccagtagt ttggtattgt accatcatct atagctgtaa
     8761 tctttgtgga agcaatctgt tgacctaatt tatctgcaaa tacagagttc ccctttgcaa
     8821 ctgatgtagc ttccaaagaa tgtccacaag cttcatgaaa tattactcca ccaaatccat
     8881 tatctattgc taccatcata ttacctgcag gacaattttt agcatgcaac atagtatgcg
     8941 ctactctagc tgattctttt gcataatatt ctgggtctat ttcttcaaat atttcaaacc
     9001 ctttacaacc tccaggacct tcaaatccag tttgattttc attaccctta gatgcaattg
     9061 aacttatccc tagtctggtt cttattcttc tatcctcaac atatatacct tcagtgtttg
     9121 caattaatat tttttggtct ttatccaaat agctaacact tacttgagat atatcattac
     9181 taaattcttt tgcactctta tatgcaacct tcataacact tactttatct ctatttccta
     9241 ttgaatttgg ataaattttt atattatgta tgttatttat tttaatactg tcatttaata
     9301 ttacactttt tccatcttcc aatttaccta aagcaacagc agctttgtat gctacatcca
     9361 acagactatt taatgaattg tcacttgtat aagcatatat actcttcaat cctttaaata
     9421 ttcttattcc tataccataa tttcttccac ctattgcatt ttctacttta ttatcaatta
     9481 aactaataga attatttata gtgtcttctt caaaaatctc agcaaaatct cctccagtaa
     9541 ttagacactt ttctaatacc ttagaagcta tattttttga aagcataaat ttacctccta
     9601 ctttaaattt ttaattaact tttattcaat ctttttactt gttaacaaac attatatact
     9661 atattactta aaataaggta attatgaata tattatacat atgttttata ctaaaatgtt
     9721 atcacatttt atctatacat acccatataa ctagataatt aaaatgtata atattatttg
     9781 ctatagaaaa taatacactc atacataata aattttagga gtgaagtata tgaatcaaga
     9841 atatgtaaaa ggaaatccta tttttactaa tattaataaa gttccaagac aatttccata
     9901 ccttactgat gatattgata ctgatgttat aattgttggt ggtggcgtaa ctggatgtat
     9961 ttgtgcttac tatctagcta aaaacaatat aaagtctgtc attcttgaaa aaggaaggat
    10021 tgcacatgga agcactagcg tgacaacttc tctattacag tatgaattag atgacaattt
    10081 gatagattta acagaggtta tgactctaaa agatgctcta aaagcatata acctttgtac
    10141 ttcagcactt gaagaattag atacatttat aaagctatat ggaaataagt gtgattatgc
    10201 caaaagagat actctacttt atactgccaa caaacttgaa attaaggcta tcaaagaaga
    10261 atataactta agaaaagaaa atggatttga tgttgaatat attgatgaat ccacaaaccc
    10321 ttttagcttt gatttaaaat caggattgat agcaaagaat ggaggacgag aattagaccc
    10381 atataaatat agtcaccatt tgattgatgt aagtttaaaa aatggtcttc aagtatatga
    10441 aaatacagaa gtaaaaaaag tagatttatc aaatgataag gttactgctg aagtatcgta
    10501 tggatacaaa gttcatggta aaaaattaat tgtagctaca gggtacaata caagtttatt
    10561 tacaaaaaga aattttgcaa ctaagtcaaa tacttttaat atagcaacta aaccacttaa
    10621 aaacatagct tcttggaaaa acaacatttt aattagagat aactgtgacc catataatta
    10681 tctaagaact actaaagaca atcgattaat tataggtggt gaagatgtaa gttttgatga
    10741 tatagaaaat gaaactttag ccaatgaaaa gtatgatatt ttagaacaaa gattgaaaag
    10801 tatgtttaag gatataaaag acattgaagt tgaatataaa tattgtggtt gttttgcttc
    10861 aactttagat aatttaggtt ttattggtcc agataataaa aataacaatc tatggtattg
    10921 cttaggttat ggagcaaatg gtatactctt tgcaatatta ggaggtataa tgttaagtga
    10981 attatatctt ggcaaacaaa ataaaaatat gaaattattt aaagtagata gatttgataa
    11041 atagatattg tatattaaaa gactataaaa agatgaaact ttataattct aaaagttaac
    11101 tttgatacag tcttttataa tgttcctttt aaaaatactt tatttataat taaatataaa
    11161 gtaaaatgtt aaaatataac tgataaaatt tagtcataag ataatattta agtttatttg
    11221 atgaaatatg gaggaattat tatgaaagaa atactcgtta ttgctcccac taaaggaacc
    11281 tatgaaaaat caattcacat agttaaaaaa aacaaatata caaatattga tgttgtattt
    11341 ggcaatctaa aagaaggtat acctcttgct gaaaaaagta ttaatcatgg tactagaatt
    11401 atcataagca gaggtggaac ttacaatatg ttaaaagcta cttacaatat acctatagtt
    11461 gaaattaaag tagatgctta tgatattata aaaagttaca aagaggtaaa aaattccaac
    11521 gaaccatttg gaataattgg atttaataat gtcatctacg gtttcgatat tatagaagaa
    11581 atattgaata agaaaataac tatgattgaa attgaaaaag aagaagaaat ttatgatgct
    11641 atagaaaagt acagaaagaa aggtataaac acatatatag gagataccac tgttgcacat
    11701 attgttaaga gacttaattg taagggaata ttaatcgaat ctagagaaga aaacatactt
    11761 agagccattc aacaagcaga acaaatactc gaagcaacca aggatgaaca aaaaagaaga
    11821 ttacagatag aagctatgac agattttgta catgatggaa taataacagt tgataaaaat
    11881 tttaaaataa cattatttaa taagagtgct gaaaaaatat ttggtataaa aaaagaaaat
    11941 gcactttatc ataatgttgt agatgttatc ccaaacacag ttcttccaca agtagttgag
    12001 actatggaac ctcaaattgg tgaaatacaa aatataggga gtacaaaaat tattacaaat
    12061 agagttccaa taattgttga tggccagatt caaggggctg tttcaacatt tcaagatagc
    12121 aaggagataa gttcttttga acatacaata agaaggagct tattaagcaa aggtctatct
    12181 gctaaataca cttttgataa tataattcat gctagtgaaa agacaaaaga ttgtatagaa
    12241 cttgcaaaaa aatatgcttg ttatgatact cctatgatta ttactggaga atctggagtt
    12301 ggtaaagaat tattttgtca aagtatacat aactatagta agcgtaaaaa tgcacctttt
    12361 gttgctgtaa attgtgctgc catacctcca tctctaatag agagtgagtt ttttggttat
    12421 gaggaaggtt catttacagg tgctagaaaa aaaggtaaag caggtgtatt cgaactagca
    12481 catgaaggta caatcttcct agacgaaata agtgaaatcc ctcttgaact tcaaggtcga
    12541 ttattgaggg tattgcagga aaaacaagtt atgagaattg gtggtgataa agttattcct
    12601 atagatgtaa aaattatctg tgcttcaaat aaagacttaa aaaatatgat gcgcaatggt
    12661 ttatttagac aagaccttta ttttagaata aatatactaa cattatatct tccttcatta
    12721 aaagaaagaa aagaagatat tataaaatta tcagaatttt ttatacataa atattcaagc
    12781 aaatacaata aaacgccttt aattatcact ccaaatatta aaaaggcact tcttgaacga
    12841 gaatatgaag gtaatataag ggaattggaa ggtatgatgg aaagggctgt tattgtggaa
    12901 tcatttgact cgattttatt agaaaaggtt agcaatgaat atacaaataa tatagttgat
    12961 aaagataata gtgattttga atgcagtaat tt
//
LOCUS       DAEPOG010000113        12869 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEPOG010000113 DAEPOG010000000
VERSION     DAEPOG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.113"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            138..1997
                     /locus_tag="KR432_002561"
     CDS             138..1997
                     /locus_tag="KR432_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0641088.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2289..2888)
                     /locus_tag="KR432_002562"
     CDS             complement(2289..2888)
                     /locus_tag="KR432_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0641089.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2991..3788)
                     /locus_tag="KR432_002563"
     CDS             complement(2991..3788)
                     /locus_tag="KR432_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0641090.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3901..4006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4098..5084)
                     /locus_tag="KR432_002564"
     CDS             complement(4098..5084)
                     /locus_tag="KR432_002564"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0641091.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5568..7298
                     /locus_tag="KR432_002565"
     CDS             5568..7298
                     /locus_tag="KR432_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBH0641092.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7408..7488
                     /locus_tag="KR432_002566"
     tRNA            7408..7488
                     /locus_tag="KR432_002566"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7442..7444,aa:Leu,seq:tag)
     regulatory      8385..8489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8699..11173
                     /gene="gcvPA"
                     /locus_tag="KR432_002567"
     CDS             8699..11173
                     /gene="gcvPA"
                     /locus_tag="KR432_002567"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBH0641093.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11173..12630
                     /gene="gcvPB"
                     /locus_tag="KR432_002568"
     CDS             11173..12630
                     /gene="gcvPB"
                     /locus_tag="KR432_002568"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBH0641094.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4819 a         1532 c         2133 g         4385 t
ORIGIN      
        1 gatgaactat ataaaacaag gtttatatat tctaatagta tagaaaattt tattgtaaac
       61 gcatgtatat tattagaaaa aatagtttga aggtattcaa taaacataaa aaacaatata
      121 ggggataaag gtgttttatg aagagatttt tgaggagaat tatcttagtt ttatttattt
      181 tgttgatttt tataagtttt atttctatta aattaattca taatgttgga gattatggaa
      241 aattgattaa ttatgttggg attgtttgtg gagctagcca acgtctaact aaactagaga
      301 tgaatcatca accaaatgat gaattaatag aatatattga tgaaatccta caggagttga
      361 ttacagggca tggagattat ggtttagtaa tcactgattg taatgaatac aatgaggact
      421 tacttttatt agaaaaaaag tgggaagatt taaatattga aattaaaaaa gtaagaatga
      481 aagaacagaa taaccagtta ttaagtataa gtgaagagtt tttttctctt gcaaatgata
      541 cagtgtttaa aatagaaaac ttttctaaag aaaaaagtaa ttacttaatg actttaataa
      601 taattatatc tattataggt atattagcat gtatcatatt gattttacaa tatagtaaaa
      661 aaatggttag actggaaaaa ttaaatgtag atttaaaaaa tattgcatat aaagatgaac
      721 taacaggagt aaatactata gaaaaattta aattggatgc aaatcaaaat atttgtataa
      781 ataaggataa gaaatttgct gtattctata ttgattttga aaactttaaa tacattaatg
      841 atatatttgg atatgattat ggtgacatga tactgaaaag atatgctaat cttatgatga
      901 atgacatagg gaaatatgaa atatttgcaa gagaaattgc agatagattt gttgcgttaa
      961 gatgctatgt agataaggaa gatttagtag taagacaacg agatgtagac aatgaactaa
     1021 ttaatataac aaatgaaatt aaaaataaac atagtattac tgttgtcagt ggaatttgtt
     1081 gcattgaaga tgtaaatgaa aaattaagta tagatggttt aataaataga gcaaattttg
     1141 cacaaaaaac tgttaagaat aaaccaggta ctaattatgc tttttataat gacagtattc
     1201 gtaaaaaaat gattgaagaa aatactatca agagtagaat acatgaagct attgaaaaaa
     1261 gagaatttat agtttattta cagcctaaag ttaatttaca tagtcaaaaa ataaattgtg
     1321 cagaagcttt agttcgttgg cttactccgg ataaaggtat tatatctcca gcaatattta
     1381 ttccagtact tgagaagaat ttttttattg ccttagtaga taagtatgtt tttgaggagg
     1441 tttgtaaatg gattagaaag aggttagatg aaaataaacc ttttgttcag atatcagtta
     1501 atgtttcaag aatacaattt tataacacta aatttgtaga aacttattcc aatatacaaa
     1561 ataaatacag gatacctaaa aatactatag aaattgaatt tacagagagt gttgcttttg
     1621 agaatcaaaa acatcttttg gaaattatac atgatttgca tgaaaatgga tttacttgtt
     1681 ctttagatga ttttggaaaa ggttattcgt ctttaagtgt tttaaaagat ttaccatttg
     1741 atgttttaaa gcttgactct atgtttttca aggctagttt agataaagat aaagagaaaa
     1801 tagtaataaa aaatattgta catatgttaa aagagcttaa tatcacaact gttgctgagg
     1861 gcattgaatg caaggaacaa gttgaatttc ttagggatat tgggtgtgat ttagttcagg
     1921 gatttgtatt ttataaacca atgccaatat tagaatttga agaaatattg gataaagagt
     1981 ttgtttataa tagctaaaat gtactttata aaatatgaac taattaagta aaattatttt
     2041 gtactgaata aatttttact ttatttacag aatagtgatt atttacagaa aagggcgaaa
     2101 agtgtattcc tttaagtatg ggatggatag cacaaatagg ggagaaaaaa tattggccat
     2161 ataagaatgc ataataatat aaatatttga aaggtcagca ttatataaaa attaaaaaga
     2221 aaataaataa tttttctata aaaaagagac tatgagaaaa gttactcata gtctcctaaa
     2281 taatgtattt attttcttcc tgcaacccat tttaagcccc aatgaaattc agtatccatt
     2341 tctctatgac ctttgaagta tttaactact ttttcaacac tgaatgtctc atcatttaca
     2401 ttttctatca atgctattga acacatatta tagccatttt tatgttcatc aagtttgaca
     2461 actaagtctg aatcttgttt ttgtttgatt gttacaattc catcagcttc agaccagtta
     2521 gctacacctt cgtatataaa agcataaata agtattcttt ttatatcttt tattttatta
     2581 ccatttattc ttaaattttc tccttgtgta ttacttcctg ttctgtcatc tccatctaat
     2641 tgagcaaaag gtggattgtc taaactacca aaagcatttc ctaatgcttg aacagcacct
     2701 tttacaccat ttttcatctc aaataggcaa cccaaatcta aatctatatt actgcttctt
     2761 aaagagccaa aaaaaccctt tttttgtact ttttgattcc aatttaaatt aactaagatt
     2821 tcacctaaat cgctattttc cttttttgta aggtttattt tttgtccctt ttttaattct
     2881 atagccatag taatcttcct ccacaaacat agatttattt aaatggtagc acttaataag
     2941 tcaatattca acaagattag aataataata ttaaaactat actaaatata ctaaaaagta
     3001 ggaatcacaa tacctttgta tttttcttca attaagcttc ttgctttatc tgatgtaaga
     3061 acatcttgta gaactttaat cttttcactg tctttatctt cagttctaaa tgctaaaaga
     3121 tttgcatact cgtcatttat aggagctgta taaattccgt ttttcttggc atcaagacca
     3181 gcaggtacag cataagtagt atctataaat gcagcagtaa catctggaag agaagatgga
     3241 atttgagcaa tatctaactc tttgaattgt attccctttg gattttctat tatgtcctta
     3301 ggactaacta atccatcccc tttaggtaac gttaaaagtc caatactttc aaggtatcta
     3361 aggttcttag agcaagaaga tgggttattg cttattgcaa ttgtatctcc ttttttaaat
     3421 tcatcaacag attttatctt ataagagtaa agtattgctg ggcatacata tagtttttta
     3481 ccagccatta ttttgtaacc tttggcctta acagcttctt ttagataagg ttcatgttga
     3541 aatagatttg catcaatttc accattattt aaagcttcgt ttggaagtat gtaatcatca
     3601 aagtttttaa cttctaaagt atatcctttt tctttaataa gtggttttaa ttcttctaat
     3661 aactctcctc ctggaactaa agtagcacca acaacaattt ttttatcatc tttagcttta
     3721 gaacatccaa ccacagctaa agttagtact agacataata acaaacataa taattttttg
     3781 aatttcatat aaattccctc ctgaatataa ttgattagtt aaaaataaaa taccattctt
     3841 tatcacactg caaaataaaa ataagcctga gtttaaactc aggctttaaa agctaattta
     3901 gactcatctt tcagtataat actgcaggat ttagcaccaa tgtatataaa aatatactgg
     3961 ttgctgggtt tcatagggcc agtcccttca ccactctgga taagaagtat caatttttaa
     4021 ttatgttata aatattatca tgatttttta cgtctgtaaa cataatttta taaatatttt
     4081 aaaatcttta atgtatttta tttaacacct aaaattccag aaactagaat atcagtatca
     4141 gcacctaaga aatagtcatt tatttcaata gaatccagag tatttctcaa agcattttct
     4201 tcatgcctta taccaataag tttattttct acaaatgcaa catcacattt tccaaaaaaa
     4261 tctccaaaaa attttatact agttattatg cctttatcaa catttaaatt aaactcaaca
     4321 tttccaccag aatattttaa ttcatttgaa agagtatatt ttggtgaatt accaaaattc
     4381 caatcccaag tttcgtattt tgatttagta agcttttcta tttgttttat atcatgctca
     4441 ttcaaacaat aattagtatt atcagtattt gttttagaaa gataatccat tatagcatct
     4501 ttaaattcta atatgtccat ttttgaatgt aaatgttcac ttatattggt tacacgagat
     4561 tttacagatt ttattccttt tccttcaaat tttataggtt taacctttaa tgcttttgag
     4621 aggtcattta tttcagatga aaataaaaga gtaccatgat gcattacttt gtttttgtag
     4681 ttgtattgag catttccaga aaatttcttg ccatctataa gaaggtcatt tcttccagaa
     4741 aattcggcat tgacatctag tgttttaagt aaatctatta tgggctgagt gaatcttctg
     4801 aaatcactaa aattattatt gttacaagct ataaatgtaa aatttatatt tcccaaatca
     4861 tgaaatacag ctccaccacc agactgtctc ctaacgacag gtatagaata ttcttttata
     4921 tattctaaat ttatttcaga aagtgtgttt tggttttttc caacaattat agaaggttca
     4981 ttcctccata aaataaataa gtcttcatca aaatttttta gaagatattc ttccattgct
     5041 aaattaaaat aagggtttgt tttttcatta taaattaata acattaaagt agttcctcca
     5101 tagaattgat aaaattttta ttatgaatac aattaatttt ataattcgac aataatataa
     5161 aaactccttt aaattagaaa ttttaaaaac taccttgcaa agtttaaata aataatatat
     5221 actcaaaata tgtttaataa aagtgaatag aagggtagag gcgcaataat ctatcagtac
     5281 taataaggag ttaagcacag agagttatta ggaaaggaga tattgccgaa gttttataat
     5341 cgatgcttta agattatatt gctgggggta catagaaaat atgtacaact gtcacaggtt
     5401 tatttgtggt gagctatcat ttaaggaatt atattatatt tatttttact attgttgata
     5461 tattgtaatg tttaagcctt gagtgtttac cactcaaggc ttttttagtt ttattgtaac
     5521 ataataccct gtacagttca caaaattaaa aggagaggga gatttatatg aggaacaaaa
     5581 aaattaatat tatttttctg acgacaatca tgttcataat gtcaacagta atggtttttg
     5641 cagaagaaga tatagacact attgcactag ctaatgcaga aaaatttggg attttaactt
     5701 taataccacc attagttgct ataatacttg catttataac taaaaacgta ataatttctt
     5761 tacttatagg tattctttct ggtagtttta taattaaagc tagtggtatt aatgtttttg
     5821 cgacatttat acaggcattt ttagatttag tagatagagc tttagtatct ttagcagacc
     5881 cttggaatgc agggataata ctacaggttt tggcaattgg aggagtaata aatcttgttg
     5941 ctaaaatggg aggagctaaa gctatagctg aagcacttgc taaaagagca aagtcagcaa
     6001 aagggacaca gcttataaca tggtttttgg ggcttttagt attctttgat gattatgcca
     6061 attcactaat agtaggtcct atgatgagac ctgttgcaga taaaatgaaa atatcaagag
     6121 aaaagctagc atttataata gatgccacag cagcaccagt tgctggttta gctataatat
     6181 ctacatggat aggtcttgag gttggtttaa tacatgatgc ttttgaaagt ataagtatag
     6241 atgtagatgc atttggtata tttttaaata caataccatt tagattttac aatatattaa
     6301 tattagcttt cattgtaata tcagcattat tattaaaaga atttggtcca atgagaaaag
     6361 ctgagataaa atctagaagt agaaaaatta gtatagacct tgatgaaggc gtagaagagt
     6421 tggatgattt agctccaaaa aatggagtaa aattgagtgt atggaatgct ataatcccaa
     6481 taggaacatt gataatagtt gcattagcat cattttatta tagtggatat acatctataa
     6541 tgggaggaga cgataaggct ctgatacaac tatttacaaa ttctccatat tcttttgaag
     6601 caataaaaga agcatttagt gcatctgatg catctagagc attatttcag tctgcacttg
     6661 ttgcaagttt agttgctatt ataatggctg tagtaaaaaa gatttttaca atatcagaag
     6721 caattgatgt ttggatagat ggaatgaaaa gtttagtaat cactggtgta atattaatac
     6781 ttgcatggtc attaagttca gtaataaaag agttaggtac agctaagttt ttaatacacc
     6841 tattatcagg ttcactacca ccattcttat taccaagttt aatatttggt ttaggagcaa
     6901 taatatcttt tgctactgga acagcttatg gaacaatggg tatacttatg ccacttgcaa
     6961 ttccattagc atactcatta aatcctgata tgtcttatgt aatagtaagt actagtgcag
     7021 ttttaacagg tgcaatattt ggagaccatt gttctccaat ttcagataca acaatacttt
     7081 cttcaatggg agctggctgt aatcatatag accatgttaa cactcaaatg ccatatgcta
     7141 tatttacagc agtaatcact attgtatttg ggtatatacc agctggactt ggattgccaa
     7201 tatatatagt attaccagtg gcaatagctg ctatatttgt aggtattcaa ataattggta
     7261 agaaagttga tgaagcggaa atagaacttg tagaataaag atataaataa gtttcacctt
     7321 ttattatcac tattttgtta gtttttagat aattttaaaa agttattgaa aataatggcg
     7381 aaatatagta aaataaaaga gtgataagcg gatgtggcgg aattggcaga cgcactagac
     7441 ttaggatcta gcgccattgg cgtgggggtt cgactccctt catccgcact ttacagtact
     7501 taattgtact gaaaaaaact ggggttgctg gctttcggag tgggctatcc cgtttttatt
     7561 tttttgtata tataatagat tggcacttta gtataaggtg ctttttttat aggcaaaaac
     7621 agtacattta tttctgtttt attatcattc atctattaga atatacttta tttaagaatg
     7681 tgttaataag cagtgttcac attcttgata aaatgtggaa taatatgcct accaataagc
     7741 atttggtgta taagtaagag tatttatata gttgattgtg aaatgagtac aacaattctg
     7801 tacagtataa ttggcattat aattgtagca ttgaatttaa agtttgcaca gtcgctgatt
     7861 ggaaaattta caaagtggat gtcaaacata taaattttag tttattggac agttcaagga
     7921 atcagaaatt ggaattttat agtattaata ttttatagta ttaatataat gtataaaatt
     7981 ttatagcaat gtatttcata agtataaagt gactagaatt ttaagaaata tacaaattct
     8041 ataaaaacta aataatttca agtatatttt aaaaatctta tttttcacaa gtttaaaatt
     8101 gtaaacgatt tcatcaagtc tatgaatata cttttaacat gttatagtaa atctcaatat
     8161 aaatttacat ttggttcttt gtgtgttatt ttaataataa attaagtgaa aatgttattt
     8221 tacattaata tataatatct ttgcataaga gtgaataata taaaagagaa agaattgata
     8281 ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata aatataatgt gttgacattt
     8341 aattgaaata ttcatacaat aaaattgaaa ataaatattt ccaaatgaga ttagcgagag
     8401 agtcctttat acgaaaaggc gccgaagaag aaatttaaga aatgccaatt tttagagaag
     8461 ctttcaggca aaaatatcgc taattgatgg gactctggag agacttaagt taagatacta
     8521 taagtcaccg aagaagcaaa ataatattaa attattaaat ctttcaggta tacaaacagg
     8581 gatatatagg ctaggattat tttccatgct gatatatctt ttttttatga aaaagtacag
     8641 gaaataatat caaagttact taaatacagt aggtctaatt ttctagggag ggttatatat
     8701 gaatgaattg aaaagagtat ctttgtacaa tatacacaaa gagcttggag caaaacttgt
     8761 tgaatttgct ggctgggaaa tgcctttaga atatgagggt ataaataaag agcatgagaa
     8821 ggttagaaaa agtgcaggta tatttgatgt atctcatatg ggagaggtac agataaaggg
     8881 agcagagtca gaaaaattta tacaaaacct agttacaaat gatataagca cattaaagat
     8941 aaatgacatt atatatacac ctatgtgtta tgaaaatggt ggagtagttg atgatttatt
     9001 aatatataaa tttggtgaag aagattattt attagtaata aatgcaggta atatagataa
     9061 agatgtagca tggataataa aacaaagtga aggatacaat gtagatataa aaaatatatc
     9121 tagtgaagtg agtcaattag caattcaagg acctaaagca gaagaaattc ttcaaaaaat
     9181 cacagatata gacttaaact caataaaatt ttataagtca attccatcaa caaaagtatg
     9241 tggttgtcct tgtttagttt caagaacagg atatactggg gaagatgggt ttgagatata
     9301 ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt ctaaaggttg gaggagaaga
     9361 tatatgtcca gctggtctag gttgtaggga tactttgaga tttgaagcag ctctacctct
     9421 atatggtcat gaaataaatg aacacatatc tccaattgaa ggtggtttat ctatatttgt
     9481 gaaaacaaat aaagaatcat ttattggtaa atcaatttta tcaaaagaaa aagaaagtgg
     9541 agctaaaaga aagttagtag gatttgaaat gcaaggtaaa ggaatgccta gaaatggata
     9601 tgatataagg attggtgata aaacagttgg atttgtaaca acaggatgtg catctccaac
     9661 gacaggaaaa attttaggta tgggaattat agattctgaa tatgcaaaag taggaaatga
     9721 aataggtata gcaatcagaa aaaaagttgt tcctgctgtg atagttaaga aaccttttta
     9781 taaaaaacaa tataaaaaag ataatattat attaaacaaa gaaaataaat tttcatatat
     9841 acctgctaca agtgaagata aatcaaaaat gttaaaagta gttggattaa attcggttga
     9901 tgaattattt tcagatatac cagaagaagt aaaattaaaa agagatttaa atttggaaat
     9961 tggaaaatct gaattagagg taagcaaaat agtaaaaaga ttgtcagaag aaaacttaag
    10021 tttagaagac ttgacttgtt ttcttggtgc tggtgcatat gaccattata taccatcaat
    10081 tataaaacat ataacttcaa gatctgaatt ttatacagca tacactccat atcaagctga
    10141 aataagtcag ggaactttac aagtagtttt tgaatttcaa tctatgattg cagaaattac
    10201 aggtatggaa attgcaaatg catcaatgta tgatggagca actgctgcaa tagaagcatg
    10261 tataatggca atgaatcaga ctagaaaaag taaaatagta gtgtcaaaaa ctattcatca
    10321 tgaaactttg tcagtattaa gaacttactt acaatataaa gattgtgaga tagtagaaat
    10381 tgacttttgt aatgaatatg ggactacaga tatagaaaaa ttaaaagctt ctgtagacaa
    10441 ggatacagca tgtgttctta tacaaactcc taatttcttt ggaattattg aagaaatgga
    10501 agaaatagaa aagataactc atgaaaataa ggctatgtta attatgagtg tagacccaat
    10561 atcattagga gttttaaaaa caccaggtga gataggtgca gatattgttg ttggagaagc
    10621 tcaatcactt ggaaaccctc ttaattttgg aggtccttat gtaggatttt tagctagcaa
    10681 atctaaatat actagaaaaa tgccaggaag aatagttggg caaagtttag atgtagaagg
    10741 taaaattgca tatgtattaa ctttacaaac tagagaacag catgtaagaa gagaaaaagc
    10801 aacttctaat atatgttcaa atcaagcttt aaatgctctt gtagcttcaa tatatatggc
    10861 tactatgggt aaagaaggat ttaaagaggt tggtatgcag tctatgaaaa aagcacatta
    10921 tacttacaat aagcttgtac aaactggaaa atataagcca atatttaaag gtaagttttt
    10981 caaagaattt gctgtacaag gaaatcttaa tatagaaact ataaatgata agctcttaga
    11041 agaaaatata ttaggtggtt ataatttaga atacaattat ccagaattaa aaaattcaac
    11101 tttgctttgt gtgactgaga aaagaagcaa agaggaaata gataagttag ttggaataat
    11161 ggagggatta taatgaaaga atacaatagt ttgttgatag atatatctaa aaaaggtagg
    11221 aaagcatact cacttccaaa gttagatata gatgatataa aaatagaaga tatgattgac
    11281 caagatatgg caagacaaag tgagttgaat ttgccagaag ttggtgaatt agaattagtt
    11341 agacattata cattattatc aaataaaaac tttggagttg atacaggttt ttatcctttg
    11401 ggttcttgta ctatgaaata taatcctaaa ataaatgaag atatggcagc acttccaaac
    11461 tttactggaa tgcatccata ccaatcttcg gatacagctc aaggttctct ttctttgatg
    11521 tatgatttat caagaagact tgcagaaatt acaggtatgg atgaagttac actacagcct
    11581 tcagcaggtt ctcatggtga gtttacaggg cttatgatta taaaagcata tcatgaaaat
    11641 agaggtgacc ataagagaac taaagtgata attccagatt ctgcacatgg aacaaatcca
    11701 gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag catctgataa aaatggagct
    11761 gtagacataa atgcattaaa agaggttttg aatgatgaag tagcagctct tatgcttact
    11821 aatccaagta ctcttggttt atttgaaaaa aatataaaag aaatagctac tcttgtacat
    11881 gaagcaggag gacttttgta ctatgatggt gcaaacatga atgctattat ggggataaca
    11941 agacctggag atatggggtt tgatgtggtt catcttaatc ttcataagac tttttcaact
    12001 cctcatggag gtggaggtcc aggagctggt cctgttggag ttaaaaaaga attagtacct
    12061 tttttaccaa taccattaat tgaaagaaaa gaagataagt atgtactaaa ttatgataga
    12121 gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt ttggagttct tgtaagagct
    12181 tacacatata tactaactat gggaagggat ggacttaaag aggctagtga aatggcagtt
    12241 ttaaatgcta attatataaa ggaaagtata aaagatgatt atattttgcc aatagatacc
    12301 ttgtgtaagc atgagtttgt attaggtgga ttgccaagag gagaggccaa tataaaaact
    12361 atcgatattg caaaaagact actagattat ggataccatc ctccaacaat gtactttcct
    12421 cttattataa atgaggcact tatgatagaa cctacagaaa gtgagagtat agagacatta
    12481 gatagtttta tagaagctat gaaaagtgtt gctaaagagg caaaggaaga accagaacta
    12541 ttgaaaactg ctccacataa tactttggtc aaaagagtag atgatgcaag ggcagtaaag
    12601 aaacctatac taacatggtc acaaagataa ataattaaaa agtagagatt taaagtataa
    12661 agagaattaa atttgtaaga aaaagctatc taacttaatc cagtttagat agcttttttg
    12721 tatattagta tgtatggtat tttctatata aagttttaaa ttatattagt gcttatatag
    12781 aatattgata tgatgattat gtatagatat taataagaat tgttgaattt atcttgcctt
    12841 aactttattt actcttttat cttaagaaa
//
LOCUS       DAEPOG010000114        12619 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEPOG010000114 DAEPOG010000000
VERSION     DAEPOG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.114"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(116..433)
                     /locus_tag="KR432_002569"
     CDS             complement(116..433)
                     /locus_tag="KR432_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641095.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            complement(540..1043)
                     /locus_tag="KR432_002570"
     CDS             complement(540..1043)
                     /locus_tag="KR432_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641096.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(1095..2294)
                     /locus_tag="KR432_002571"
     CDS             complement(1095..2294)
                     /locus_tag="KR432_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBH0641097.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(2554..2742)
                     /locus_tag="KR432_002572"
     CDS             complement(2554..2742)
                     /locus_tag="KR432_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641098.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            3077..4045
                     /locus_tag="KR432_002573"
     CDS             3077..4045
                     /locus_tag="KR432_002573"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBH0641099.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(4162..5292)
                     /locus_tag="KR432_002574"
     CDS             complement(4162..5292)
                     /locus_tag="KR432_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0641100.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(5445..6398)
                     /gene="msrA"
                     /locus_tag="KR432_002575"
     CDS             complement(5445..6398)
                     /gene="msrA"
                     /locus_tag="KR432_002575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH0641101.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(6522..7427)
                     /locus_tag="KR432_002576"
     CDS             complement(6522..7427)
                     /locus_tag="KR432_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0641102.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(7791..9383)
                     /gene="hcp"
                     /locus_tag="KR432_002577"
                     /gene_synonym="priS"
     CDS             complement(7791..9383)
                     /gene="hcp"
                     /locus_tag="KR432_002577"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH0641103.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(9443..10168)
                     /locus_tag="KR432_002578"
     CDS             complement(9443..10168)
                     /locus_tag="KR432_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0641104.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            10309..11037
                     /locus_tag="KR432_002579"
     CDS             10309..11037
                     /locus_tag="KR432_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0641105.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(11201..12472)
                     /locus_tag="KR432_002580"
                     /pseudo
     CDS             complement(11201..12472)
                     /locus_tag="KR432_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         4203 a         2189 c         1335 g         4892 t
ORIGIN      
        1 aaatatttaa gacgatttta aaatttcctt aaaatttcct taattttcaa acaaaaaatc
       61 aaaaaagcct acacttaaat gtgtagactt taataaattt tcatacaaat acattttatt
      121 gaataacgaa caatcttaat gtagaaaaaa ctataactac agttaaaaga agtctagcat
      181 ataaaaaatt tttttctttc aacataactc ctgcaactcc aaatataaaa aatagagaag
      241 caataaattt tccaataaga ttattattag gataataact aaaaagcata aaacaaagta
      301 caaatgtaag tactcctatt gcattaaaca caattttatt agatgctatt tttcctttat
      361 tgaaaaagaa acagaatatt gacactaaat ctaaaaataa aaatacataa aacattcctt
      421 ttaaaacgcc catttttata ccccctaaaa attctaatta taatatccag tttatttcat
      481 ataatcgtca atataatcaa tactttataa catcagtata acagtattac aaatttatat
      541 tacgaatgta ttacttcgta ttcatacata ttataatcac actcatacat atttaaagac
      601 tcatatgttg cttttgcatt tatgttttct ttttctacat ataatctcat tccaactata
      661 ttttcatctt tatcacatat attttttata taattaaata aataattaaa gatacctttt
      721 cttctatatt ccttatctac atatacactc tgaatccaaa ggaaatttcc atttctccaa
      781 tcactccact catatgtata cataatctgt gcaacaactt tatcaaatac tgtatataca
      841 tgatattttc cttttctctc atctaataac aatgctttta cacctttagt taaaacatcc
      901 atatcaagtt cttttccttc agtttcttta gccaaattat aattaaattt tgctattgta
      961 tccaaatcat ccatacgtgc ttctcttatc tctattagtt cttcattttc atataactct
     1021 atgtagtcct tttgttgctc catacttatt cctccatatc tattttatct actaaggttt
     1081 tttatataat atatttatag agttttttct atcttcataa atgcataatc aagtgtagcc
     1141 tctaaatttc cattattctt acctcctcct tgagctaata ctttacttcc accacccttg
     1201 ccatctataa gacttattgc atcctttaat aaaactccca tattaatatc ttttaaattt
     1261 ttagaacatg caaaaactaa atttatttta tcattaaatt taacagctaa taatgcaata
     1321 ctattttcat attctgcaat tttatttgct atcttattta catatttcaa atcttcatca
     1381 tcataaattt ttttaatgac cgaaatattg ccaaccttat ttgctttttc aatcatttct
     1441 ttgacctgat atgtagatac tacttcttca agccttcttt ttttaattaa agcctcttcc
     1501 actttactat ttagatttac aataccattt atagcatctt cctcactaca gcttaaatat
     1561 ctacatattt tagtcaaata tatatccctc ttcaatactt catccactgc ccttttacct
     1621 gctagaaact ctattcttgt acttgactta tgtttttccc atttctttat ttttatcatt
     1681 ctcaattcac cagttgattt tggatgcact ccacaacatg cattcgtatc taaatcacct
     1741 atttttatta ctcttatctc ttcatcagta tttggcaaat cacgtcttaa ccaaatcttt
     1801 tttagttctt ttttcgttgg aagtaatgtc tctacttgta tattttggcg aataatttca
     1861 ttggcaaaca actctatctc tctaattttg ttctcttcta aattaccttc tatatctact
     1921 gtactatact catttcctaa atgaaaacct gtcgtatttg cattaaaagt tttaaaaaaa
     1981 catccagata atacatgttg tccaaaatgt tgatgcattc catcttctct tctatcccaa
     2041 tcaatactac actttacctt atgtatcttt ataggttttt tatctaatac atggtatata
     2101 ttttttccat cttcatacac atttattaca tcatcattct ctatttttcc caggtctcca
     2161 aactgacctc ctccaccagg aaaaaaagct gtcttatcta ataaaacatg atacttattt
     2221 tctacttctt ttatttcaat tatttctgct gtaaaatctc ttatatactg atttgtataa
     2281 tataactttt ccattataat tctatctcca ttttcttaat tatagactac tttacttttt
     2341 aaatacaaat ttcaagctaa gcttaattta attgtattaa aatagaactc atcctattat
     2401 ttactaacat cttctactat attttaacat atagatatta gtgttaacaa atttataatt
     2461 ttatattatt tcaatatttt ataacaagta tgcttactta ttttcttata taataaaatg
     2521 tatattaaaa catattaaat tgcaaaataa gcttcaatct cttttactaa tttcatccat
     2581 tattaatgat tttgaaatat acattgcttc tataatattc ttaaaacgtg tatgatgaga
     2641 tgtctcctca gaaaatttct tctgtacttt ttcacaatta ataatccttg aagatgctac
     2701 tctcagtact tccatcaact cttcttttgt atgataattc ataacacatt tactcctttg
     2761 ttttttaatt tagagaaaca gtatacctca atttaatatc attatcaact gatatcatta
     2821 ttatttatat atatctttta acacttaaat attctaacta tcagtgacat tacatatttt
     2881 aataattatt aatatacata taatacttta acattcatta atagagagta tattaacatt
     2941 gcttttttaa aaattttagt ataaaatcat attaaataaa ttttctgaaa taataaaata
     3001 ttaaaatatc caaaaacagc ttttgataac atctataatt ttcaatgtat cgttatttta
     3061 gggggagtga tttaaaatgg caataaagcc aagaaaaata tctattgtag gttcagggca
     3121 tgttggttct cattgtggat ttagtttaat aactcaagga gtttgtgatg aactctttat
     3181 gattgatata gatgaatcta aatcaaaagc tcaagcttta gatttagctg atgcagtttc
     3241 atatctacca cataaagtac atatagaaaa aggaactttt tctgactgta aagattctga
     3301 catagtagtg ataagtgttg cagactcttc agaagggcct ttacgtagac aaaacacaac
     3361 tagattagat ttactaagac caactattgg aatgataaaa tctatagtaa aacccatagt
     3421 tgattctggc tttgatggta tatttgtagt catatctaat cctgttgatg tagttacaaa
     3481 ttatatttgg gaaaaatctg gctttcctaa aaataaagtc attggaactg gaacagcttt
     3541 agattcaaca aaacttagaa ggattttatc tgaagaaact ggaatcgctc aacaatcaat
     3601 tcaagcttat tctatgggtg aacatggaga ttctcaaatg gttccttggt ctcatgtttc
     3661 aattggtggt aaaccaatct tggacatgat aaaagacaac ccaaatactt atagcaattt
     3721 agacttacca tcaatagttg aaaaaaataa aaaaacaggg ataagtataa taaatggtaa
     3781 agattgtaca gaatttggta taggtactgc tttagtagaa attgtaaaag ctatactaca
     3841 caatgagaaa aaagttcttc cagtttctac attactagaa ggtcaatata acgaaagaaa
     3901 tgtatttgca ggtgttccat gtgttatagg aaaagatggt attgaagaaa ttattgaaat
     3961 caatatgact gaatatgagc aaaacgaatt taataaatct tgctctgttt taagagaatg
     4021 tattgcactt tcaaaaacac tttagattca atattctttc ttacactaaa agcaggtaaa
     4081 tgacttattg aaaagacttt taactaagtt aatgtttaaa tcatttatac ctgcttttaa
     4141 ttttaatact aaaatataaa cctatttttt accatcaaat tttataactt tacttttttt
     4201 atttacctct tgagataaca atcctttttt caatcccatc aataattcta agtcttctgg
     4261 ataaagattt attccaaatt cctctaaact tgatactata ctatttacac tcattatttt
     4321 attttctatc aataattcaa ctgctctttt taaatacgtt gggcttgaac ctttcatttt
     4381 gtctagtggt tcttctatca accagccttt tttatccatt tcattcataa gataattgta
     4441 ttttttgtaa tttatctttt ctagattata tgctctaaaa atcatagcag atataggtac
     4501 aatccatttt ttctttagtt ctacataata atctaaatct tctggatgag tcaaatcttt
     4561 taaaaaactc tcttttggca ataaaaatgc acaggcatac tcatcttctg aaaattgctt
     4621 acttggtatt cttaattcac ttgatattat atatccaagc tcacatgcca aagtataatt
     4681 tctaattgat gctgactttt tgtcattacc cagagatatt agatatttag attctttact
     4741 aattctctgt ttttgagtaa agatagttgc cccttttcta tctacattca tacctgatat
     4801 tataatccct ttagattcta acaaacttac catatttact attggagtac tatttaaatc
     4861 ccaaaactct ctagtcttat atgcaagaga ttctatatca cttatattaa tatctgacat
     4921 tacttttcta tttggcaagt tttcttttgg aaacttaata taattctcca taaatgaata
     4981 tattttatgt attataatta ttttttctct ataagaaatc tcttctactc taggtagttt
     5041 agattgaggg ttaaaatggc tatcctctac aacgatattt atattttcat ttttataaaa
     5101 atactctttt ggaaaattta atatattaga taattttaat gtattttcta tatttggtac
     5161 atacttattt tcctcaaaag cttttaaatc ttttttattt attttagttt ctttagacaa
     5221 ctgttctatc gtctttgctc taaaaattct agctgatttt aatctctttc cattaaagtc
     5281 ttttaaattc atttgttttc tctctttcaa aattaaattc ttaatctatt ggtataaata
     5341 agattacaat taataattta tcatattatt gacaaaaaaa caaaaggtga aaatattttt
     5401 ttatattttc accttttaga taagtttata ataaatattc ctaattactc aaaaagtttt
     5461 aaatattctc catatcctaa aacttccata ttttctcttg gtataaattt aagagaagca
     5521 gaatttatac aatatcttaa acctccaagt tcttttggac catcatcaaa tacatgacct
     5581 aaatgagaat ttcctttagc acttcttact tcaattctgt acataccttg tgagaaatca
     5641 cttctctcaa tgatagattt atctataact ggctttgaaa aacttggcca cccacatcca
     5701 gaatcaaatt tatcttttga agaaaataac acttctccac ttacaatatc aacataaatt
     5761 ccctctttgt tattatcata atattcattt tcaaaaggta cttctgtcat atcattttga
     5821 gtgacttcaa attgtatagg agttaaagtc ttttttaact cttccctatt tatattgttt
     5881 ccatcccaat gactttttac aaaattatat cttccagacc ctttataata catattgtag
     5941 tttaatggtc tttttttata ataatcctga tgattttctt ctgcttcgta gaatttagtt
     6001 gctggaatta tatcagtcac tattttacta tcaaatattt tactattttc cagatgttct
     6061 cttgactcta aagctatatt tttttgttct tcatcatgat aaaatatagc agtcttatat
     6121 tgagttcccc tatcaccaaa ttgtccacca tcatctgtag gattaatctg tttccagaag
     6181 atatttagaa gttcatcata acttattata tcatcatcat atgttatttg aattgcttct
     6241 aaatgaccag tagttcctga acatacctct tgatatgtag ggttttcaac atgaccacct
     6301 gtatatccag aaactacttt aataactcca tcatacttat cgaaaggttt taccatacac
     6361 caaaaacatc cacctgcaaa agtagcaagt tttatcattt tcatcactcc ttaaataaat
     6421 tatactttat ttattcccta agaagtaact taataatctg cattttatta tttttaccta
     6481 ctcaatgaaa tcatgtgcaa aaatacaatt gcaaattgaa tctaatttct ataaaagctc
     6541 tttttcttta tccatccaaa agcatctttt atatcacaat tttctcctaa agacattaga
     6601 tattctaatg catcacaatc atccatagct acatcttttt ccatcttagg atttaatcta
     6661 tagtaattat ctccaagaag tttttgagaa aataactgac tcatttgaga atttccttct
     6721 aaggtaatag atataattgg taaatcgggt tctttattaa ttatccagtc aatagctccc
     6781 cattcagtag tatctgcctt tatactatta tatacatatc cagtccctat tgaaaaaagt
     6841 cttgtattct taagtttaaa tccactgtca atggcatgaa ttatacacgc taaacttggg
     6901 tcagttgcta ttattcctcc atcaacatga cgattatatg ttggaaaaaa tactggagca
     6961 gcactacttg ccattgctac atctacaact ttgctagtct ttgtaaaaga atttggcata
     7021 ttgttataaa acactggttt ccatgcattt tcctcatttc ctatataaaa acttggtatc
     7081 attactaatt tatttaaatc ttttaattct agttcctctg gaaatatact taataaaact
     7141 tcttttaagt ttttgttttc atactttggt ctgagtattt ctgcataact tttattaaaa
     7201 atatacttag aattttcaat tgagtaaagt tcttttatct ctttagggct tacaccatac
     7261 gcaagtccta gtgcaattag actccctgta gatgtccctc caaacatatt tatattgttt
     7321 aatagatttg gatactcttt tactatcctc tcaagtatgg atatactcaa tgcccctttt
     7381 aatccaccac catcaaatgc caatatatta aaattagaac ttttcatata catatacccc
     7441 cacataaaac accttatatt ttaatcaaaa taatttcact atagtatata tattttatgc
     7501 aagttacata ttacaatgtt cctcttttaa tatttaagtt acattcaaac ctttagtact
     7561 taaaataaaa tatataagat attatatatc taagtattat aaatttaata caaaaattct
     7621 taaaagaata tactgaaagc aggtttattt ttcaattatt acaaaaaata aataaagggt
     7681 cgtctcaaaa ttgatttttt aatcaaattt taagtttaac cccttatctt aattgtgaca
     7741 tacttaaaaa tattttaact ataaatatct ttaagtatag atatatttat ttatcctaaa
     7801 atcttcttca tatcttcttc aggagtagat ataggtgtca atgcaaattt atcaaccaat
     7861 atatctaata cattcttaga taagaatgct ggcaaagaag gccctatata gatatttttg
     7921 atacctaaat gaagtagtgt aagaagtata caaacagctt tctgctcata ccatgacagt
     7981 accatgctaa gaggcaaatc atttactcca cattcaaaag catttgccaa tgaaactgcc
     8041 actttaattg cactataagc atcattacat tgtcccatat ccattaaacg aggtaagcca
     8101 cctatatccc caaggtctaa atcattaaat ctatattttc cacatgcaag tgtcaatata
     8161 acagtatctt taggagcttg ttttacaaat tcagtgtaat aatttcttcc aagttttgca
     8221 ccatcacaac ctccaaccaa taaaaagtgt cttatagctc cactctttac agcatctact
     8281 actttatctt gtacagatga aactgtacct cttgcaaaac cagtagttaa tgtatctcct
     8341 ccattaatac cagtcatatg aacatcttct ctatatcctc caagttcaag agctttttct
     8401 attacactag aaaaatcttt atcttcatca atatgaacca tacctggata agcaactact
     8461 tctgtagtaa atactctatc tctataacta tctttaacag gcattaaaca gtttgttgta
     8521 aataatattg gagcaggaat attatcaaat tctttttgtt ggttttgcca tgctgtacca
     8581 aaattacctt ttagatgttt atattctttt agttttggat atgcatgtgc tggtaataac
     8641 tcaccatgag tatatatatt tataccctta tctttcgttt gttctaataa tttgtgtaaa
     8701 tcataaaggt catgtcctgt aactacaata aatggccctt tctcgatttt catagatacc
     8761 tgagtcggtt ctggtgttcc ataagtttca gtatttgctc tatcaagcaa ttccatacat
     8821 tttaagttta tttctccagt tttcataact agacctaaaa gttcgtcaat agacaagtct
     8881 cctccaactg ctattaatcc ttcatagaaa aattcattta cgtctttgtc tgtataacca
     8941 agtaccattg catgatatgc ataggcagcc atgcctctta atccaaataa gattaatgat
     9001 tttaatgaac gtatatcctc attatcattc cataaatcct tcatatcata gttatcattt
     9061 ctattacaag ttgttgaaca atctgaacat ctaggtacaa gtttactttt ttctacctca
     9121 gttttttcaa tttgtctttt tatagcttca tcatcaaaac ttacattcgt tatagtagtg
     9181 aatagcccct ctatcataat tttatctgtt tcacttgttg gtctattctt atttccaata
     9241 gttgctcttg aaagaccaat caacactcct gtaagctcgt cttgcaagtt agctgtaccc
     9301 ccctttttac cacaaacacc atttatagta caacctttac ctcctgctgt ttgttcacat
     9361 tgaaaacaaa acattttttc cattttttat tcctcccttt ttatttatta aaatttatta
     9421 tatttttcta tgattcactt aattaaattt ctccatctac aatttttcca tctacagata
     9481 tagtaactat ttgccatggt atcatttttc cactattttg tagagctgtc ttaacagcat
     9541 ttactatacc accacagcaa ggaacttcca tacgtgttac tactatattt ttaatatcat
     9601 tttcttttaa aatctctgtc agtttttcag catagtcaac ttcatcaagt ttagggcatc
     9661 cgattaatgt aaccttattt ttcataaatt tattatgaaa atttccatat gcatacgctg
     9721 tacagtctgc tgctattaaa agacttgcat tctttaaata tgatgcatta gttggtacta
     9781 attttatttg tactggccat tgattaagtt gagaaccttt tatctctact atatcattgc
     9841 ttatagatgt actatttgaa acctctctat ttattgactt agactgagaa cctggacatc
     9901 cacatgctaa agttttcttt tgtgctttct ttgcttccat atttgctttt actgcagctt
     9961 catcatattc tgctgcttct ctatactcaa atgaaatcgc atttgttgga caagttggca
    10021 gacaatctcc cagcccatca caatagtcat ctcttaaaag ttttgctttt ccatttacca
    10081 ttcctatagc ttcttcatga caagcttcaa cacatagtcc acaaccatta cactttccct
    10141 catcaatttt aattattttt ctcatcattt atatcacctc ataaaatttt tttaatttaa
    10201 agtattgatt tcttatatat ttatttttaa tacctatatt tctttatttc aaatttcttg
    10261 tagtaggatt tttatatccc cttgctttta taaacttagt atagtacaat gaaaacaaat
    10321 tatcggttgc atatgcaaca aggaagtgat tatttgaaaa aatttttaga aaagcatata
    10381 gacctactta aaaaaattcc tctatttgaa ggaattaaaa gtgatgagct tgaagaaatg
    10441 ttggaatgtt taggtgttgt agataagact tacccaaaag atagtataat attctctgtt
    10501 ggaagtgaag ttacttctat tggaattatg cttaaaggaa gtgctcatat aataaaggaa
    10561 gatatcgaag ggaataggaa tattgtagct gaactcttgc ctggagattt atttggagag
    10621 gtattcgcat gtactagact acataaaagt aatgttacag taaccacaac atcttcttgc
    10681 gaagttttat ttataaagtt taaaagtgta actggtattt gttcatctgc atgtgtattt
    10741 cataataggc ttgttgaaaa tatgctacaa ttaatagctg aaaaaaacat tttacttcac
    10801 aataaaatag aattactttc tcgtaaaaca actcgcgaga aattgcttat gtatttttca
    10861 aaacagatag aacaaacagg tagccaccag tttacaattc ccttttcaag aaatgaaatg
    10921 gctgattttt tatgtgttga cagaagttct atgtcaagag agcttggcaa gatgcgagat
    10981 gaaggcttgt tatcttttaa taaaaataaa tttgaaatct tttactttca aaaataaact
    11041 agatatttaa acatatttat ttcaatatat actatattag ttaattcaaa ttataaatat
    11101 catatttgaa tcttaaattt atatttttac ttaaaaagtt aatagatatc taatttatta
    11161 aaattaagat acctattaac tttattaatt aagtgtacaa ttagcttatt ttctttgtag
    11221 taattgctgt tttatctata agccaatctt ttccttctac taatcttgaa actaacattg
    11281 atgatactgt atttccagtt gtatttaata tagtattagg aatatcagtt atagtagtta
    11341 taactgttaa caatggaatt gcctccactg gaaaaccgaa gatgctaacc acgagcatct
    11401 caccaataag tcagccacct gctacaggag ccataaatac tccaacaaca aatacagtta
    11461 taaataattg acctatcgtt gctggatttg ccattccact gcctaaaata cctgataaaa
    11521 atgccacttt tattattact gctaaaactg acccatcttt atgtatattt attccaagag
    11581 gtataactgt ttctgatata tcatcaggaa ctcctatatt tttactagct tctaaatttg
    11641 atggtataca tgctgaactt gaagaagtag ctagtgccgt tgctattggt tttatagcat
    11701 ttctccagaa ttgtctaaac ccttgttttc ctgcagataa aaaagcatac agactgtaaa
    11761 ataataagaa atacatggct gctgttagtg aataaattac aaacaccctt acatatcctt
    11821 gaattatttt gcttccaagt tccccaatta ttgtagctgt ataacagcct agccctatag
    11881 gagcataaag catgactata cttactactt ttaatattgc ttccgttcct tctgataaaa
    11941 actttcttat tgtatttccc ctatctccca tcatagctat tgctgttcca actaaaactg
    12001 caaatataac taattgtagt aaattgctac tggaaaataa atctccaaaa tttttaactg
    12061 taatacttga tactacttgg tctaataacg ttccttttga ttctaaagtt tctgcatctt
    12121 gagccaccat taaacttttt acacttgata catctaatcc tttagacgga ttaaataata
    12181 atgcagatgt aatacctaaa aatgcaatta caagtgttgt tcccataaat actatagctg
    12241 tactcttcat tatttttcca aaacgttcca tattttttat actagcaaca cctgaagaaa
    12301 cactaagaaa aactaatggt actactatca ttaatagagc atttatgaat aaatctccaa
    12361 atggttttag tattgaagct tttggaccaa gtactaatcc tactatcacc cctattatag
    12421 tacatgctaa aagtataatt gatgacttat aattttttaa aaagtccctc atttatattt
    12481 tcctttctaa tgttttatat tataaattac cctaaatcct aaaaaaatta aagtaaatta
    12541 tctaatgcgc attaataatt tttgtaattt tccccaacag ctactttagc aaatactgga
    12601 atatatgtca atttctata
//
LOCUS       DAEPOG010000115        12504 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEPOG010000115 DAEPOG010000000
VERSION     DAEPOG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.115"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..534
                     /locus_tag="KR432_002581"
     CDS             <1..534
                     /locus_tag="KR432_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641106.1"
                     /translation="ARLLLLNLSMDNSQNNQEAFSILFEINTLYEEYIGILIKSIWDN
                     SFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIIDTKWKAIEVDSNVF
                     YRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFKGKFIEAKTVRLDD
                     IKNTKNDLKKIIFNYKF"
     gene            861..1901
                     /locus_tag="KR432_002582"
     CDS             861..1901
                     /locus_tag="KR432_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0641107.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            2257..3579
                     /locus_tag="KR432_002583"
     CDS             2257..3579
                     /locus_tag="KR432_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0641108.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3877..5238
                     /locus_tag="KR432_002584"
     CDS             3877..5238
                     /locus_tag="KR432_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0641109.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            5311..6705
                     /locus_tag="KR432_002585"
     CDS             5311..6705
                     /locus_tag="KR432_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641110.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6767..7237
                     /locus_tag="KR432_002586"
     CDS             6767..7237
                     /locus_tag="KR432_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBH0641111.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            7240..9456
                     /locus_tag="KR432_002587"
     CDS             7240..9456
                     /locus_tag="KR432_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0641112.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            9539..10210
                     /locus_tag="KR432_002588"
     CDS             9539..10210
                     /locus_tag="KR432_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0641113.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            10573..11787
                     /gene="dpaL"
                     /locus_tag="KR432_002589"
     CDS             10573..11787
                     /gene="dpaL"
                     /locus_tag="KR432_002589"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0641114.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11914..>12504
                     /locus_tag="KR432_002590"
     CDS             11914..>12504
                     /locus_tag="KR432_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0641115.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4782 a         1403 c         2480 g         3839 t
ORIGIN      
        1 gctagacttc tacttttaaa tctatctatg gataatagtc aaaataatca agaggcattt
       61 tctatattat ttgaaataaa tactttgtat gaagaatata taggaatttt aatcaaaagt
      121 atatgggaca attcatttag agaaacatat atacaagata agagtaaatt tcttttgaaa
      181 aatgaacaaa caggtaagaa aaactttaat ttaagaccgg atatagtttt atatgactta
      241 aaaaatgaat atgaaattat aattgacact aaatggaaag ctattgaagt agattcaaat
      301 gttttttata ggtcaagtga tatataccag atgtatgcat atataacagc atatgaaaat
      361 gcaaaaagat gtatattatt atatccttgt attcaaaaag ataagaatta tagttcctgg
      421 aaattatcag aatcatttaa gggtaagttt atagaagcaa aaactgtgag gttagatgat
      481 ataaaaaaca caaaaaatga tttaaaaaag ataattttta attataaatt ttaaaaataa
      541 gaattttaaa atttataaaa gtaattttat ataggtataa aaattacgag tagtataaaa
      601 aatttaataa gaacttaaat taaaaatttt aaaaattatt ttcatataaa atatggcatt
      661 gtaattgctt atttaataga ggatttttgt aaaatgaaga aagtatatct atcaaattaa
      721 aactaagtag atatactttt tattaagttt tgttttaaaa taatattatt aaattataac
      781 taagcttttc tgttatgata taattaaata gataatgtga atgattatta tatagaatac
      841 ttatttggag ttgatatttt atggacaaac acagtgattc agtaatgtat ggtggaatgg
      901 aagtatataa tatttgggga acaacaacag tagactccat aattggagag tctgatgtaa
      961 tgcgtgctct taagaataga atttgcaaaa ttggaaatag tgattctact gtagctataa
     1021 ctggagaaag tggcactgga aaagaattaa tagcaagagc cattcatctc gcaggaaaca
     1081 gagggaataa aacattcgtt gctataaact gtgctgctat accagaacct ttactagaaa
     1141 gtgaactttt tggatataca aaaggagctt ttagtggagc agattcaaaa ggtaaaattg
     1201 gtaagtttga attggccaat ggaggggttg tatttttaga tgagatagga gatatgccaa
     1261 ttcaactaca atcaaagtta ttgagggtat tacaggaaaa gaagtttacg agaataggtt
     1321 caaatgaact aattgatata gatgttagaa tcatatcagc tactaataga gatttatttg
     1381 agttggttaa ggaaaataaa tttagagaag acttatacta tagattgaat gtaatacctt
     1441 tagaagtgcc tccacttaga gaaagaggag acgatataca aatattaata aataatttta
     1501 tagacaaata ttcgagaaaa atgaagaaaa acgtgtacca tatagaacca gatgtggaac
     1561 aaatgttgct taagtatcct tggccaggaa atattagaga gttggaaaat actatagaac
     1621 tggcattaaa ccttcttgag gaagatggaa taatacataa aggaataata aatagaaaga
     1681 tacttgaata ttttaagtta aataatataa atataaagtt acttgaggaa agtgactatg
     1741 agataagtat ggaacaagac atacttacac ttgaagagtt tgaaagagcc tatataaaga
     1801 aagtgttaaa gttttacgga aatgatacta agtctaaaaa actggctgcc aaaaaacttg
     1861 gaatatcact agctacattg tacagaaaaa tagatgtata ataattaggt tttttattct
     1921 caattctaaa atttgaaatt ataaaattga taaaaaacac ttggaaaact attttctaag
     1981 tgtttttttg tgtaatagca ttttattatc caaaaataaa aaaaatttaa aagaatttaa
     2041 aaaaataaat gctttaataa attatcaaat actaacaaat ataataaaaa ttatatatgt
     2101 aaaacaatta tgttaaaaaa ttggcattat aattgcttca tattaaattg agatgacaaa
     2161 ataaaaaaag aaaggatgtt gtgtgagata ataataactg tcattaaaaa atagttggga
     2221 gtgataacct tgagtaaata tcaaaagaat gtttctatgt ttgatattga tggaaggcca
     2281 ccaattttaa agtgtttgcc actatcaatg caacatatac tagcaatgat agttggaact
     2341 gttacagtac ctatagtcgt aggaggagct gtgggtgtta gccctacaca aataacactt
     2401 ttagtgcaat atgcattaat aatagctggt atatcaacac ttatacaact atatccaata
     2461 ggaaatattg gttcaagatt gcctataatt tttggagttg gttttactta tgtaccaaca
     2521 cttgtagcag tcggaggaaa ttatggatta gcaagcattt tgggtgcaca attaataggg
     2581 ggagtggcta cagtattaat agggatattc ataaaaaaga taagaaaatt ttttccgaat
     2641 atagttgcag gaacagtggt ttttacaatt ggattatctt tgtatcctat agcaataaat
     2701 tatatggcag gtggtgtaaa tagtcctact tatgcctcta tgcaaaactg ggcagtagcg
     2761 gctataacat tagtggtagt tataggtctt agtcaatttg caaaaggata tgcaaaattg
     2821 gcagcaatat ttttgggaat tatagttgga tattgtgtat ctttagttct gggtataata
     2881 aattttgacc ctattagaga ggcaacttgg tttgcgatac ctaaaccgct tgaatttggg
     2941 atggaatttg atatgcctgt aataatctca gtagtaattg taagtataat caatgctgtt
     3001 caagcaattg gagatttatc agctactaca atgggaggca tggataggaa tatcacagat
     3061 aaggaattat caagtggagt aataggaagt ggaatttgta ctatgatagg agctttgttt
     3121 ggaggtttac caccatcatc atatagccaa aacgtgggga tggtagctat gaacaaagta
     3181 ataagtagat ttgtgcttgg aatagctggt atatttatgt tattgtcggg gtttgtgcct
     3241 aaatttggtg cattaatgac tactatacca tattctgttt taggaggagc tacaatatct
     3301 atatttagta tgataacaat gacaggtata aagttaatta ttacagaaga gttgtcatca
     3361 agaaatatta ccattgttgg tttggcaatt gccttaggca tgggaataac atcagtacca
     3421 gcagcccaag aggcatttcc ttcatttgta aagttagtat ttggagaatc tccaatagta
     3481 attgcaacat tggttgcatt cgttttaaat ctaataatac caaataaaag tctagaggat
     3541 gaggctaaag aaagagctgt tatggaagag actaattaag atataataat ttaaaagaat
     3601 tgtttaaatt atttagtatg agattgttag attatttttg atagctattc attttaataa
     3661 aaaatttaac aaataaatat ataccaggca attaatgttt catttaagtt gaaaatcggg
     3721 gagggaatat ttagaaattt gttaattttt tataaaaata gctcagttta gctgtgttta
     3781 tcggttgagc aataaattca tggtcataaa aatatttata gaaaataatt ataaaaatat
     3841 ttagtcaaga tatttatgta tgggaggatt tattttatga aaacacgtaa tacttcaaaa
     3901 tatgatgtcg atgggatacc accactaaag gaatcaatac ctttggcctt acaacatctg
     3961 ttagcaatga tagtaggaaa tatggtacct gcgatattaa tagcaaacgt ggtaggactt
     4021 aaccaggggc aagcaacaat gttgattcag ggttctatgt tagcagcagg tttagcaacg
     4081 tttttacagt tatatccaat acctttattt aaaggattta agttaggttc aagattacct
     4141 gtaatgatgg gaatgagtta tgtattttta ggagcttgtc tttcagtagc agctaaagat
     4201 ggtttagcag ctctatttgg agcacagatt gctgctgggg taatcgtatt ctttgtagga
     4261 tttggagtta agaaaattag acatatattc actccaatag tttctggaac tataatagca
     4321 tgtatgggac ttggactatt cgcaacagct ataaagaatt tagctggagg agaaggcaca
     4381 caaacatttg gaagcccaat aaacttcaca gtaggagtta tagttgcttt tgtaataata
     4441 atgataaata aatacggtaa aggtttagta aaaaattctt caatattaat aggaatatta
     4501 gtaggttatg ctatatcttt agttttagga ttggttgatt tctcagccgt tcaaggagca
     4561 gctatagtat ctttaccaac accagcagct tttggattag aatttagacc agagttaata
     4621 gtaatgttta ctattattta cataataggt atagctgata tgatgggtgc ctgtacaata
     4681 gctacagtag gagcaatgga tagagaggta actgacgaag aattagcatc agttgtttta
     4741 ggaaattcta taacttctat aatttcatca ctatttgcag ctcttccaac tggagtattt
     4801 agccaaaata cagtaattgt atctatgaac aaagttacaa gtagatttgt aatagcacta
     4861 ggggcacttg tattattact agcagggata tctccagcat tgggtgcaat aatgacaact
     4921 ataccatctt gtgttgttgg tggagcaact ttagtagttt tctcatcaat agcaatgtct
     4981 ggtttctcta ttatgagtat ggatggattc actgaagaaa acaatcttat agcaggtgta
     5041 tctatagcaa caagtatggg tcttacaact gctccacaag ttttagacca attcccagaa
     5101 acaattggaa ctgtccttgg agggtcaagt atagtttctg gagcaataat agctatatta
     5161 cttcaaacat tatttaaatt aaagtcacgt aaaagtgctg aaaacgttac ttcaaatctt
     5221 gaggaaaata ttggataaat gtattagata taatatgaga aaaccatatg gtaaaatgtt
     5281 gtaatgaaag tattcaaagg gggatttaag atgattgata tactaataaa aaatgcaata
     5341 attgttacag ttaataaaga cagagatgtt atatttgatg gagcaatagc tattcaaggt
     5401 gacagaatct tggatatagg aaatacagtt gatattgagc ctaagtatct tgatgctaaa
     5461 aaagttatag atgctaatgg aaaagtaata ttccctggct ttataaatac tcataatcac
     5521 ctgttccaag tgttattaaa aggtttaggt gatgatatgg cacttcatga gtggttaaat
     5581 acaatgatgt ttccatcagc aaaattctta acagaacaag atacttatga tgctgcaatg
     5641 cttggatgta tggaaggttt aaaaagtggt ataactacaa tggttgatta tatgcatact
     5701 cacaatagac caggacttac agatggaata gtaaaggcat ataaagattt aggtataaga
     5761 ggggttgtag gtagaggttg tatagattta ggtattcaca aagagcttat agaagatgtg
     5821 gaaacggttg aaaaagattt gagaagggta ttcgaaaaat atcataattc agaaaatgga
     5881 agaataaaaa tctgtgtagc tccttcttca atgtgggcta tatctgaaga aatgggtaag
     5941 atgctttgga atatagttaa agaatatgat tcatatttta cagttcatat atctgaaaca
     6001 gaatttgcaa gaacagcaac aaaggatata catggagaaa tagatataaa attacttgaa
     6061 aaatggggta tagtaggtcc agaagttgta gcagtacatt gtgtatgtat aactgatgaa
     6121 gacatggaaa tgcttaaaaa gtatgacata aaagtttctc ataatgtagc aagtaatatg
     6181 tatcttgctt ctggtgttgc acctgtgcca gaaatgttaa aaaaaggtat aaatgtgagt
     6241 ttaggtcttg acggtgcagc aagtaataac gcacaagata tggtagagct tatgaaatta
     6301 acagctttac aacacaaagt aaataattgt gaccctctgg caatttctgc agagaaagtt
     6361 cttgaaatgg ctacaattga gggagcaaga actcttagaa tggaagatga aataggttct
     6421 ttggagattg ggaaaaaagc tgacctagta atatttaact caatgctttc tccaaaatca
     6481 atacctcttc acaatcctgt atcaactttg gtatactcag ctagtatgca caatgtagag
     6541 agtgttttag ttgatggaaa tgtaatactt gaaaatggaa aagtaactac aattgaaagt
     6601 gaagaacaag tgttgcttaa tgcacaactt gcagcagaag aattatgtaa gagagctaat
     6661 gtaactaata gaatggaagg acataagtgg aatagcttat actaatatta aaataagatt
     6721 gtaataatat tctattacaa cattagttta tctgtaggag gagaaaatgg aaaaaataaa
     6781 attaaatatt aatgattaca tgtatgaaat agaagttgaa aagaatgaaa ctcttttgca
     6841 tgtacttcgt gaaaaattaa atctaactgg agcgaaacaa ggttgtaata gtggcagttg
     6901 tggagcatgt aaagttttga tagatggagt tgacatgaaa tcttgtaaac ttcttgcaaa
     6961 aaactgcgaa ggtaaagtta tatacactat agaaagtttt gctaatggag aaaatcttca
     7021 tccaatacaa gaagctttta tagaagttgg agctgtacaa tgtggatatt gtactcctgg
     7081 tatgattata acaacacaag ctcttttaaa tagaaatgca tctcctaatg aagatgaaat
     7141 taaagaagct tttaaagaaa atctctgtag atgcacagga tatgtaaaga ttatagaagc
     7201 tgtaaaatta tctgcagcta gattaggggg tagataatta tggaaaaggt aataggaaaa
     7261 tcataccctc taaaagatgc agctttaaaa gttactggtc aaatgaaata cgtggctgat
     7321 atgaaaccac aaaatgtact ttacgctaag atgcttttaa gtcctgtagc acatgcaaaa
     7381 ataaaaagta tagatacaag tgaagctgag aaagtagtag gtgttagagc tatagctact
     7441 tatctcaata catcacaaag aaaatacaat ggttcattaa gattttatga gcacaatata
     7501 cctgaaaatg aagttatatt ttcagataca gttagatttg ttggtgaccg tgttgcagca
     7561 gtagctgcag agactccaga aatagcagag aaagctgtaa aacttataaa agttgaatat
     7621 gaagaattgc cagctgtatt tactatacaa gatgcaataa aagaagatgc tatctgcata
     7681 catggagatt caaatatagt ttctcaaaac aacataaatg ggggagatgt agaaaaaggc
     7741 ttcaaggaat gtgactatat atttgaagat gaatactcta ctccagcaat acatcatagt
     7801 gcaatagaga ctcatagtgt aattgcacac tatgattata atgataaatt aactgtacat
     7861 acaccttgcc aaaatacatt tggatttaga ataatactat ctcaaatttt tgggatgtca
     7921 ttaaataaag taagagttat aagacctgct attggaggct catttggtgg aaaattagaa
     7981 cttacaattg aaccagtagc agccgcatta tcaaaaatga caagaagacc agttaaaatg
     8041 gttcttacaa gaaaggaatc tatgatatca acaagaactc gtcatgggtc ttttgtaaaa
     8101 ttaaaaactg gtgtaaataa agatggtgaa gtaatagcac aagatataaa aatatataca
     8161 aatacaggag catatgcatc aagtgcttta aatgttatag gagcacttag tcataaagta
     8221 tttaaagttt ataaaatacc aaatataaag tttactggga tgcctgtata tacaaataca
     8281 ccaatagcag gagctatgag aggctatggg tcacctcaaa tatttatggc tcaacaggct
     8341 caatttgcga aaatagccaa ggaaataggt atggatttag tcgattttca aaataaaaat
     8401 gcagtagagc cagatgatgt cgatataatc ttccgcggtt ctcttggaaa tcctagagta
     8461 ttggattgta tagagcaagg taagaagatg tttaagtggg atgaaaagaa aaaacttcca
     8521 aaagaagaag gaaaatattt aagaggtata ggtatggcaa taggtgcaca tggtaatgga
     8581 gtttttggtg cacatagaga tgttgttaca ttgactctta aattaaatga agatggtaca
     8641 ttgacactac taacaggagc acatgatatg ggtaatggtg tcgtaactat gcaaacaatg
     8701 atggtagctg aagttttagg aataacacct gataaagttg atacatttga aactgatacg
     8761 gatgcatgta cattcaacct tggtgactat gcaagtagag gggtatttgt agaaggtgga
     8821 ggagctaaaa agacagcaga gaaattgaaa aaccttatag tagaagaagc agaaaaatta
     8881 cttgaaacat caaaagaaga tttatatcta gacaatggat atgtaattag taaacttgat
     8941 gaaaatgtaa aagcatcact ttctgatgtc gcagtatata gtcaaagtaa atcattaaga
     9001 gagttaactg ttgttgaaga ttattcttca ccagctggac ttacttctta tggtgtacat
     9061 tttgctgaag ttttagtaga taaagaaact aaggatataa aagtagttga ttttgtagct
     9121 gtacatgatg ttggtagagt tataaatcct ttaaatttag aaggtcaatt agaaggtggg
     9181 attcatatgg gtctaggata cgcattgagt gaagaattgg catttgatga acaaggtaga
     9241 cctaaagcta ctgattttaa aacatataaa ttatttagaa ctgtagatat gccaaaatgt
     9301 caagttgcat ttgtaaatag ttatgaaaaa gctggacctt ttggtggtaa gagtattgga
     9361 gaatgtgctg ttgtaccagt tgcgccagct gttgcaaatg cagtatataa tgcaactgat
     9421 gtagaaatac attcattgcc aataaaatta aaatagtaga aattaaagca caaacaacaa
     9481 tttattgtat ttgtaaatag ataataaaaa tagatttatt tggtgaggag atattattat
     9541 ggcaaagata ttaggtattt gtggaagtcc acgtaaagga gcaacagaat atgcagtgtt
     9601 agaagctttg aaagaagcag aaactatacc tggtatagaa actgagtatt ggtcagttag
     9661 agggaaaaaa ataagtcctt gtgtacattg tgatgcatgt ataagaaaag aaactatgtg
     9721 tattataaaa gatgatatcc aagaattaga gcaaaaaata ttggaggcag atggaattat
     9781 agtaggttct ccagtttatg atatgaatat aactgctcaa cttacagcag tatttaatag
     9841 acttcgtcca atgtatttag ttcatccagg aaaacttcaa aataaggttg gtgcagctat
     9901 aactactggt ggaactagat atggtggcca agaattgaca aaacttccta taataaattt
     9961 cttcctaatg catgaaatgt tagtatctgg aggtttaggt ggatgctata taggtggtac
    10021 aatatggagt aaagatggaa aagcaaaagg tgcccaagag gatgagacag gtatggacac
    10081 agtaaaaaga cttggaaaag gtgctgcaga ggcagttatg gtttctaagt ttggtcttga
    10141 aaaatggaat gtcgtaaaag aagaattaga tgttaaaaaa gatgaaaaat cacctcttag
    10201 agaccactaa agatttatta tattattaaa tatatacaaa tagtgatgta atagattatt
    10261 gcatcactat tttatgtttt aatttagatt ttataaattt tatattaaaa aatatagaaa
    10321 caaagtgttt tgagatttat ttttaaattt taaaaatgag aattaaaaag tgagaagata
    10381 gaaaaggttt tttagttatt aaattctaca tacattgtat gaactaaaga tagaaaagaa
    10441 agaataataa aagcattgca atttctatac taaagagtca tagaaaaata tctagtatat
    10501 atttagtgtt ggcatgttaa ttgcttatat attaaagtat aaaaatttaa caataatttg
    10561 gaggtatttt agatgttaaa agaaattaaa tggaaagtaa ataatttgcc aaagggtgat
    10621 aaggaaaatt gtatcaaatt tttaaatgaa gaagaaataa ctaaggttag aaattttcac
    10681 aaaagctttc ctcaatataa agaaactcca ttagcaaacc tagaaggttt agcaaaaaaa
    10741 ttaggtgttg caggagtata tgtaaaagat gaatcttata gatttggatt aaatgcattt
    10801 aaagtattag gtggttctta ttctatgggt agatacttag ctcaaagatt agatacagat
    10861 atatctgagt tagggtatga caaattaact tcgaaagaaa taaaagaaaa attaggagaa
    10921 ataactttct ttacagctac agatggaaat cacggtagag gagttgcttg gacagctaat
    10981 aaattaggtc aaaaatctgt tgtattaatg ccaaaaggtt cttctgagtt tagattaaat
    11041 aaaattaaag gagaaggagc agatgctagt ataacagact taaactatga tgatgcagta
    11101 agattagcta atgattatgc agaagctgat gatcatggag taatggttca agatacagca
    11161 tgggatggat atgaagaaat tccagcatgg ataatgcaag gatatggaac tatggctcaa
    11221 gaagcgatag agcaattaaa agaatatgga gtagatagac caacacatgt atttgtgcaa
    11281 gcaggggttg gttcacttgc tggagcagtt caaggatatg ttgcttcaat atacgatgaa
    11341 tgcccaataa cagtagtagt agaagctgat gaagctgatt gctactataa atcagcagaa
    11401 gcaggagatg gaaaaccaag atttgttgga ggagatatgc caactataat ggctggtctt
    11461 gcttgtggag aaccaaatac tataggattt gaagtattaa aaaatcatgc tgcagcattt
    11521 gtttcagcac cagactgggt ttctgctaaa ggtatgagaa cattaggtaa tcctttaaat
    11581 ggtgatgaaa aggtaatttc tggagaatct ggagcagtta caactggtct tttagtagca
    11641 gctatggaaa gagaagattt agcagattta agaaaagatt taaaattaga tgaaaattca
    11701 agaatacttt taataagcac agaaggagat actgaccctg acaaatatag aagtatagtt
    11761 tgggatggag aatatccaag tatataagga attactaaaa tgcaattatt agatattggt
    11821 ttacatattt agaagatata taagttctaa aatttagaaa atatataagt atataaaata
    11881 catacattat aaaaaatcta gggggataat gaaatgttaa acgaacaaag aaaacaagaa
    11941 gtaactgaaa tatgccaaaa attaattcaa aatcctagtt cttctggaaa tgaagaaggt
    12001 gttgtaaaag caatagaaga gtcttttgaa aaattaggtt ttgatagttg gtcaagagat
    12061 agatatggaa atatagtagg atgtataaaa ggtaacaagc caggtaagaa aatattattt
    12121 gatggacata tagatacagt tccagttcca gatgcaagta aatggagtgt tcctcctttt
    12181 gaaggaaaaa tagttgatgg aaagatatat ggtagaggaa cttctgacat gaaaggtcaa
    12241 tatactgcaa tgatgtctgc tgtagcgtat tttgctaaag acactaatcg agattttgct
    12301 ggagaactat atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt ttcggctaga
    12361 gagataagta aggctgttca acctgactat gttgtaatag gtgagtcttc agagttaaac
    12421 ttaaagattg gtcagagagg tagaggagaa atagtagtag aaacttttgg taaaccagct
    12481 cactctgcta atccagaaaa aggt
//
LOCUS       DAEPOG010000116        12432 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEPOG010000116 DAEPOG010000000
VERSION     DAEPOG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.116"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..657)
                     /locus_tag="KR432_002591"
     CDS             complement(<1..657)
                     /locus_tag="KR432_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641116.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     V"
     gene            complement(651..1358)
                     /locus_tag="KR432_002592"
     CDS             complement(651..1358)
                     /locus_tag="KR432_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641117.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            complement(1662..3503)
                     /locus_tag="KR432_002593"
     CDS             complement(1662..3503)
                     /locus_tag="KR432_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBH0641118.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            complement(3716..4600)
                     /locus_tag="KR432_002594"
     CDS             complement(3716..4600)
                     /locus_tag="KR432_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBH0641119.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            4808..5725
                     /locus_tag="KR432_002595"
     CDS             4808..5725
                     /locus_tag="KR432_002595"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBH0641120.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            complement(5854..7188)
                     /locus_tag="KR432_002596"
     CDS             complement(5854..7188)
                     /locus_tag="KR432_002596"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBH0641121.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            7530..8918
                     /locus_tag="KR432_002597"
     CDS             7530..8918
                     /locus_tag="KR432_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0641122.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            complement(8999..10207)
                     /locus_tag="KR432_002598"
     CDS             complement(8999..10207)
                     /locus_tag="KR432_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641123.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(10222..11640)
                     /locus_tag="KR432_002599"
     CDS             complement(10222..11640)
                     /locus_tag="KR432_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0641124.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            complement(11658..>12432)
                     /locus_tag="KR432_002600"
     CDS             complement(11658..>12432)
                     /locus_tag="KR432_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641125.1"
                     /translation="KINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPG
                     EFEGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEE
                     KLSFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKL
                     TLSNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQY
                     MKKINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
BASE COUNT         4172 a         2094 c         1318 g         4848 t
ORIGIN      
        1 tactaatgat tttacacttt ctttttcttt ttctaattta ataaacttta tctttaaact
       61 tctattaagc tcatatagac agttatttat cttactaaat gtaccttctt taaaatactc
      121 attatattct actagataac cagtttcttc tgactcagta gtgtccttca agtagttttc
      181 ttgtgccatc ttatctacta taaaataaat tttactcaat cttttattta tgtatcttat
      241 aaaataatat attataccta taactactat aaacatcatt aaaaaaataa aaaatgattc
      301 cttaaaaaaa gattttaagt ataaactaaa atttttatca tttgacattt tattttccaa
      361 tccatagcca aatttcttaa atgccttttc tccagtagac accttactat aatcggaatt
      421 ataaatagaa tttactatat ctatttcatc attaggatat ttttcaatca atattccagc
      481 aatattttga gtatttttta tttgatgttc atacaattta tgtaatctgc tatattcaaa
      541 aagtataaat acaagggtta ctattagtac agataaaaca gaatatacaa taactcttct
      601 cattatataa tcatctttca cacttaaacc atctttgagg ttcatatttt ttagcatttt
      661 tttatactcc tttctcccca catatatcca acccctctta ctgtttttat atatttaggt
      721 tcagatggat ttttttcaac cttttgtcta agtcttctta tatttactgc aatcgtatta
      781 tcatctacaa aattaccact actatcccat aaagattcta acagttgttc tttagtcaat
      841 gtctgcatag aattttccat caaatatttt aatagttttg tctctgtctt acttaaaatt
      901 atctcttctt ccttatcatc tgttcttata attaatttat tttctatata ataaaagaat
      961 aaatcatcac aaactaatag agtataattt tttactgtat ttactctttt cataagcgca
     1021 ttcacttttg atattaatac catcaagcta aaaggctttg tgatataatc atctgcacct
     1081 aaatcatatc ctgtgacaat atccacctct tcatcaagag ctgtcaacat tattatgtaa
     1141 acatcacttt tctttctaat ctcctcacaa aagtcaaaac cactaccatc aggtagacct
     1201 atatcggaaa taattaagca tatctcttct tttgcgaaaa tattttttgc ttcttctata
     1261 ccaaatgcac taaaaacatt gtatccttct ttttttaatt taaaactaat tcctctattt
     1321 aaagatttgt catcttccaa aagtaatata ttattcatcc ctatatcctc catcatcata
     1381 tatactagct aaattatatc atactaataa aatcattttt ttatcaattt gtaaatatat
     1441 cagaagttgt taatatgatt tcatttactt gaataaaaac taaaaatccc cataatacta
     1501 tgccatttag tgtaattatt tttcataaaa tacaatttta tttaaaaata atcatgttaa
     1561 agcataaaac aaaggaaatt agtatttata taactaattt cctttgctgt ttactattat
     1621 ctaaaattaa attactatct actaattttg ctcattaatt tttattttga ctttttatcc
     1681 tttaactctt tttgattaaa attcaataaa catatcaatg tacttgaaac tataaatata
     1741 attatactat ttgaaaaacc taaatctcct gttttaggag atttttcttt agcttctctt
     1801 ttagatattc cttgattaga actttctcta gcacttgttt tatctaagct tttatttttg
     1861 tcactatcag agctttttat attattagat agattctctt cattatcatt gatatttttt
     1921 tcattttcac ttaatatcaa atcactctca tgattcttgc ttattaactc atctttagat
     1981 ttaataccaa ttcttccatc tacatttttt gatatttctt ttttgtactc tatatattca
     2041 atgactgcac tctctaaatt tccaaattcc tttagtattg gctcttttga aaaacaagga
     2101 aattcatctc cacctgaggc tatataatca ttcgttgcaa ctacatactt tttatttaaa
     2161 tctaatggct ttccttcttt ttgaatactt gttattcttt cgccttcttt ttgtttagga
     2221 ttaaagtagt atgaaattcc tcccacttgc aaaaaggctg aacttttttc tggataaagt
     2281 tttactccat gctccaatac atctttaatc tgtgctcctg taagttcttt ggtaactata
     2341 gtattgctaa aaggaaatac atctacaata tctcttcttg ttatattgcc tttttctatc
     2401 gtatctcgaa tattccctcc attaaataaa gaaatatcag ccttagtttt atctagtaaa
     2461 atgtcactta ttaaattacc caaattagtt tcttttgtcc ttacattttc atatgaacca
     2521 tctagagtat ttgtagttgc acctataact ttatctaata ttttttcttg ttgttctttt
     2581 atttcatcaa cctttttact tacaacgtag tcaagcttat actttaatgc ttcttctttt
     2641 ccaattaatc taactgattt attttctatt tttatatttt ctacatcatt agtatccaaa
     2701 gttagtttta tctcgccaat attttctaaa tattgtcctg cacttacaat caatgtatca
     2761 cccacttgtt taccattttc aaacttagtg tggctgtgtc catcaataat caaatcaatt
     2821 ccttgaacct catttgctat atctatacta tttggcttac ttaaactatt tgtaccaata
     2881 tgacatattg caactattac attggcacct tttcctttta attcatttac ttgttctgtt
     2941 gcactttcta taggattttt aaactctaat ccttccacat ttttaaatcc agttttgttc
     3001 tttgtttcct gggttgagag tccaaaaaat cctattttta ttccatctat ttcttttata
     3061 tcattactat caaaaacttt ttttccattt tcaaaaatat ttgatgataa aaattttaaa
     3121 gtattctctc ctaaattagc acttttagat aactcaaaca gtctatcttt tccataatta
     3181 aagtcatgat ttccaggagt aatatagtca tatccagcac atttaattaa ttcaattgca
     3241 cttttccctc tatccatatt tactattgga agtccatgca aacaatctcc tgcatctact
     3301 aaaattgagt ttggagtttc ttttttcaat gtggctagat ttcctatttg tatatggtca
     3361 tcaccttcag catatcttcc atgtatatca ttagtgtgaa aaatggttat ttcttcaaat
     3421 cgctctagcc cataagaaat aaaactctga cttgttgtaa ttagaattat aattaccaaa
     3481 attaaatacc ttctaatctt caatataaat tagcctcctc aaattagttt tagctttaat
     3541 ataaaattta tattttacat aaaacttttt agataaatat taaactatag taagattgat
     3601 tagtgtaatt tttaatattt atctatcaat ttaattttgt ccatatccaa ttagttttaa
     3661 tctaattaca ttttttcaca tctatatttt tcttttatta tataaaatta ttattttaaa
     3721 atcttttcaa ctggtctctt ttaaaagtcc attgaggtat tgtcaaatca ccttcaaaat
     3781 ctaaactata ccaaggactc aaatttggat ttttacgatt aataagaagt tgcgtttgtt
     3841 gtgcaggcat atagctttgg gctatcataa ataccttttt accagtcttt tcattttctg
     3901 ccatatccat aattatactt gcatgtcctg gacttccacc aactataaac acatctccta
     3961 ttttcatatc ctcaactttt acaggctcca attctttttc aagagataac gttcctgaat
     4021 atgcaaacac tatatccata aactttctga aactctcata agatgttgaa tattcagttg
     4081 ctttatacca gcttgaacca ctatctttta ctgaaattct atatccttgt gcccattttg
     4141 aatacttcgc cttaaaacca tcaacaaaat caaaacttat ttcatcatat agtttattct
     4201 gatacaagta ttctgctctc ataagcataa tcgcatctgc acattgatgc aaatctctat
     4261 tgccaatatc aacatcaaat acactatcat aaataccttt tgattcctta attcttccat
     4321 cataatactt tactttctct ccataatttt ttagcttagt attcctaaga aatgattcaa
     4381 aactaccttt ttctacatca actcttttaa atccttttgg tggctcatac ctatctttca
     4441 atatatttgc atctttatgt actattatag gctgttcatc ttttttagaa ttttgaattt
     4501 tcttagaacc ttcaaccact tttttatctt ctccgttact acaaccaact aaggttaatg
     4561 taatcaatga caaaatagta attttcttta atactttcat taaattttcc tcctttaaat
     4621 actcctaaat ataattttca cttttcgttc tccaaatttt tatacattgc tatataaaaa
     4681 ctttatttga taatatttct tgactcaaat acacatttat tgttatattt aatgttaaaa
     4741 taattataat aggatatcat aataaaaaca aacaacagat ttgtgttaat tttagaaagg
     4801 ggaaaaaatg atagatttaa ctttacttgg atgtggtgga aatgtaccta tgccaaacag
     4861 atttctatcc tctgtattta taaactataa aggtagaaaa atactaattg actgtggtga
     4921 aggtactcag gtatctatga agctaaaaaa atgtggcttt aaagatatag acttgatttg
     4981 tataacccat ttacatggtg accatatatt tggactttta ggacttttat caacaattgg
     5041 gaatagtggt cgtacaagtg atttaactat agttggtcca gttggaatag tcgattgtat
     5101 acgttctatg agaaatttag tagaatatgt accatacact ttaaaaatta tagaaaaccc
     5161 tcaaggcaat ttttctttag ataataaagt tttaagaaat ttagaaattt caacaatcag
     5221 tttagaacac tctatagagt gtttaggata cagttttaat tttagaagaa accctaaatt
     5281 tgatatagat aaggcaacta aaaacgaagt tcctaaaata ttatggaaca aactccaaga
     5341 aggtcaaaat atagttttaa attcaaaaca atacactcca gatatggtct taggagaatt
     5401 gagaaaggga gttaagataa gtcttacaac cgatacaaga cctatagaat caataccaga
     5461 tttcataaaa gatagtgatt tatttatatg tgaagccatg tatggtgatg atttagatat
     5521 atcaaaagct gttagaaaca aacatatgac ttttagagaa gctgctaatc ttgcaaaact
     5581 tggaaatgtc aaacaacttt tactgaccca ttttagtcct tctttagaca tacctagtat
     5641 gtatttagaa aatgctacta atgtttttga aaatactatt cttggagaag atagattaag
     5701 cttgcgttta aattttgatg aataatttaa tggaatcctt atttctaaaa atttggaata
     5761 aaaatttagg gatgaatttt accatgataa aaatagttta ggtaaattca ttcccttaat
     5821 tgcttgtcaa ctatattagc tataatctaa atattattta gacacatttc cagctacatt
     5881 taaaacatcc catgttcttg caaatggtgg ggcataacat aaatctaaca ttccaagttg
     5941 acttgtggtc atttttccaa agatagcagc tgccaataca tttgctcttt ggacagcatc
     6001 cttaaaccca gccacttgtc cgcctaaaat aacttttgta tctgcatcat aaataagttt
     6061 tacatatatt ttatttcttc ctggataata atgagtttga ttataatcag aaataaattt
     6121 agatttgaca tttatgccta aatctttagc ttgcttttct gtaattccag ttgatgctgc
     6181 ctccatatcc ataattttta tacaacttga agatagtgaa ccttgataag atacttctct
     6241 tcctgctagg ttttctccta ctatcctacc taatttatta gctccagtag ctaaaggaac
     6301 atatacattt tcattactta ctatattctt tatagttgca caatctccag cagagtatat
     6361 atcttctaca gaagttcttc catattcatc aactactata gctccatttt ttagcatttt
     6421 tatattagta tttgacaaaa actcggtatt aggtctcaca ccagtagcaa ttattactac
     6481 atctgtatct atttcccctt tgtttgttat aactttttct actttatctt ctccacaaag
     6541 ttctacaacc atttcatcca agtgcaaatt aacattatgt ctatttattt cttcttctaa
     6601 tacatccgtc atctccttat caaatacttg tggcaatatt ctatcttcta actgaattac
     6661 atctacatct ttacctaatt tcttacatgc ctcaactgct tctaacccta taaaaccagc
     6721 tcctattatt gtcactcttt tattatcttc attacttaaa agttttttta gatattctcc
     6781 atcatataaa ctttttaatg tagaaacatt cttcaatttt atgtttttta taggaggcat
     6841 aatacttctt gctccagttg ctatcatcaa tttatcatag taatcttcat atatttcatc
     6901 tgtttttata ttttttactt ttattttctt agattcacta tcaacctcaa cgacttctct
     6961 aaatatattt aaatcaattc cagcttctct aagtttctct ggtgttcttg ctaataattc
     7021 atcagcatca tcaaaaaaac caccaacaaa gtatggtaat ccacaagcac caaaagatac
     7081 tatttcagtt ttttcataaa caacaacttc atactcaggt tttattcttt ttaattttgc
     7141 tgcagcactc ataccagctg caacaccacc aattattact actctcattt tatctctcct
     7201 ttgatgtcta gttaatttct tcacttattt taagcttttc atttgatata tattctatgt
     7261 aaacattttt cataacaatt tttagttatt tatttaacat attttaattt gaaatcaaat
     7321 attataaaag ctatttttta ttttcttacc acaattcaaa ttctttaaac atttattata
     7381 ccctattttt ctatacttaa tcacattttt caaaatcaat ttattttact ctatttaagt
     7441 aaattgtgat ataatattga cattttttta atatattggt atcatttaaa cttagaaaaa
     7501 tttattttta aataaaagga gaaaaaatat tggaaagttc atttttatca caatttctta
     7561 tgattacaga ttttaaaacg atttttttca taatattact tctaggtaca ttctttattg
     7621 ttagacaatt tgaaaagaaa aaaataaaat tttcattgag aacaatatat gctacagtac
     7681 taggactcat attgggaatc ataatacaag cttttgctgg ttttcctaat gataccactc
     7741 aagtcacttg gctacaagaa gtaagcaagt ggtatagcct atttggttct ggatttatgg
     7801 acttattaaa aatgttagta gtacctttag ttttcttatc cattataaaa gtaattataa
     7861 atatgaaaga taacaatctt ggaactctga catttaaatc tttaggtatg cttcttgcta
     7921 ctactgctat agctgcaatt gttggaatta tcgttgcaaa tataatgaag ttaggtgtag
     7981 gagctaatat gcctacttta gttgaaaatc aagaacttaa agaaataaac tctcttgtgg
     8041 atactttaag aggattacta ccatctaatc ctgtaatggc tatggcaaat ggaaatattg
     8101 tagctacaat aatatttgct acatttatag ctacctcaat aaagagatta agtaaaaaac
     8161 actttgaaac aataaaacca tttatagatt ttatagaagc atcttacaaa ataattgtaa
     8221 gtgtgtctat gactgttatt aaatttatgc cttatgctgt ggttgctctg cttgcaaata
     8281 caataacttc acaaggtatt agctcaatag tatctgtttt acagtttata ttagctcttt
     8341 atatatcagt ggcaatatta tttaccatac atttaatttt tataacttta aatggtctaa
     8401 atcccattac ttatataaga aattcaactg aagctttact tctggcattt acttcacgtt
     8461 ctagtttagg aacattgcct gtaacgatag aaagtctagt acaaaatcac ggagttgatg
     8521 agggtgtatc aagctttaca gcaagtttag gtgcaaatat gggtatgaat ggttgtgcag
     8581 gtatatatcc tgctttaatg gctgtaacac ttgccaatat ggctggagta aacatggata
     8641 tgagccttta tgttatgttg ttaattgtta taacaattag ttcgtttgga atagctggtc
     8701 ttccaggaac tgctacaatg gccgtttcag ttgttatctc tggagttggt cttggttctt
     8761 attttccatt agttggtggt atacttgcaa tagaccctat actagatatg ggacgtacca
     8821 tgataaatgt taatggtgct atggtatcat caattactgt aggaaaatca cttggaaaaa
     8881 tagataaaga agtatttggt aaaaaaaaca tttcttagag taacacttaa gataaatggc
     8941 taacttttaa ctagttagcc atttattttt tattttataa gaaaatttct ttagaaattt
     9001 acttacaaaa cggaaaaaat tcttttgtaa gattttgggg atatacagat ataaaaacaa
     9061 agtcatagtc atgggatttt gcatattcta gtatatctcc atcataatat ctagggtcta
     9121 tcatatctat agatgaacaa acagaggaaa aaaatgctgc tgttggtaca attagagaat
     9181 ctttaacaaa cagcactttt aatccatctg gattttctat attttttatt ttaactaatg
     9241 gtctattccc caataaatat gtaaaatact tatctgattg accatccatt aaatcaaggt
     9301 ctgcattaaa aggatatgat aatattaaac tctcttcaaa ccttcctttt aattcaaatt
     9361 tttgagattt agaatctgta taataagtat aattagtctt aaatttagga taaattaatg
     9421 taaaatcatc tataccacca tataagatac cagtctttct tcccatagaa ccaagaaatg
     9481 acttaggata tacaattgag ttataatttt ctttatctct atagtaatga ttttcatcta
     9541 aattcatatt gtatttttta ttaagttgct caactaattc tccaaatgcc caaaaagaag
     9601 tttctacttt ccagtgatgg tctgttttaa aaaataaatc atcttttgga ataccactct
     9661 ttaaaatatt ttctctaaaa tcaatatagt ccacattata cttttttagt tgatttaaaa
     9721 aattatctgc tgtctcattg ctataagaat aaggtattcc ttttggaaat tttgtcactc
     9781 cttttatata cttatctgga gtcattaagt aaactaattt agtgttttgc ttttccattt
     9841 cctcagaaaa ttttcttacc ctatctgcta tcacaccaac tggatttgga gctttagcaa
     9901 aatatgtata atgcatcttt ccagaagtat cttttacaac actaaatttt gaaaactcat
     9961 ttttacccat taacttttgt aagtagccat aagtttcaat gaacgtattt ttatatatta
    10021 ctttgtcatt tactgtgtta tcaatagatg atattgtatc ttttaaacta cttttttcag
    10081 caactgaatt ttttatctcc tgtatcatat cttttccaga ataacaaata ttaaagatag
    10141 agaaagcgaa taaaataatt aagaacataa ttgcagttat taatcttact ttaaaaaatc
    10201 tattcatact tttcctccca actaaaaatt aaaataaata aatggattat agcttccagt
    10261 tactaaatat gtcacagaaa taaagaacaa taaaatagct gagattggat aaaatacgtt
    10321 aaatatcata ctatatttag cttgttcaac aacaaactta ttgattttct ttgcaatagg
    10381 tacactaaaa ataaatgcaa atataaagaa aacccagtat tcttttaaaa acatatatgt
    10441 ataatcactc catataactc cattgcctcc aagcatagct ttgaaataat tatatgcttc
    10501 ttttaaatta ggtgacctaa acaataccca cccaaagtta actactaata acgcataaat
    10561 atgttttata aaattgtgat tttcaatctt ttcaaacatt ataaatcgct ctataatcaa
    10621 gaaaacaaag tttaaaatcc cccaaattac aaatgtccat tctgctccat gccatattcc
    10681 agtaaataac cacactataa acatatttct aatcataata tctttatttg ctactctaga
    10741 acctccaagc ggaaagtata tatagctctt aaaccacatt cccaaagaaa tgtgccatct
    10801 tctccaaaac tcactaattg attttgatat atatggataa ttaaagttct cttcaaattt
    10861 aaaaccaaac ataagaccta aacctatagc catatctgaa taagcagaaa aatcaaagaa
    10921 tatttgcata gtataggcta ttgaaccaag ccatgcaagt gatgcagcta tttctccttg
    10981 actattcata gtgtaaatat agtctgctac tatagccatg ttatttgaaa ttaatacttt
    11041 tttgcctaga cctgttataa acctgcaagt cccaatagaa aatttattcc aagtttcttt
    11101 tctattgaga atttgttctg ccacactctc atatctaact ataggacctg ctattaattg
    11161 tgggaaaaat gctatataca gtcccacata aaaaggattc ttttggactt ctccatgtct
    11221 acgatataca tctataacat aagacattcc ctgaaatgta aaaaatgaaa ttcctatagg
    11281 taaaacaata ctaggtatag ttagttcagt attaataagt tcactaatat ttcttagcgc
    11341 aaaagctaga tatttaaata taaataaaac acctatatta tatacacaca tcaaaactaa
    11401 aaaaactttt acttttattt tactttccct atatctatct actagtaaac caaatatata
    11461 gttcattatt atggaaacta tcatgataac aacaaatttt ggctctcccc aagcataaaa
    11521 aaataaacta acagcaagca atatcatgtt ctgtatcgtt cggttaaacc taaatacata
    11581 atataaaacc aaagttatag gtaaaaaata aaataaaaaa attaaacttg aaaataacac
    11641 aatctaacct cctctcttta cttagttttt gaacattttt tccaatataa atatattcca
    11701 aagcatatta ttactagaga tacaaataaa attcgaacaa acgttatgat gttattcata
    11761 aagtttattt ttttcatata ttgtactgtt ggtgattgct caggagatac caatgtatat
    11821 ggaacaccat ccttatcttt tttattaata ctccaagatt cagttaatgc tattttcctt
    11881 ccaattttaa acttatcatc aacattaact tgtaaaataa tttgtccagg ttcggcttta
    11941 ttacttaatg ttaatttacc attttgagat atgctcacat tatcttgttt tcccataaga
    12001 gaatacttaa ttttagaatc acgtttaaga cctaatacct catactgact tatactttga
    12061 cccaatactg gtatttgtac ttcttcattg ccagttattt taatattatg taaaaaactt
    12121 aatttctctt cagacaataa agatatcttt tttataataa caatattatg ttcttcatca
    12181 gatggaataa atgtaattcc ccaactaaca atgtttgaaa gcatacttga gtccagcaca
    12241 acattacttt tctcatttat aaggctatta aagttcacat aaattttgcc ttcaaattca
    12301 cctggtactt ctatgcatcc ttcttttatt ttatctgaat atattatatt tttatcttcc
    12361 aaaaatattt cactaccttc ttttaaccta aattcaagca tattcttact ttgtatactt
    12421 aaatttattt tc
//
LOCUS       DAEPOG010000117        12327 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEPOG010000117 DAEPOG010000000
VERSION     DAEPOG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.117"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            228..1157
                     /gene="glsA"
                     /locus_tag="KR432_002601"
     CDS             228..1157
                     /gene="glsA"
                     /locus_tag="KR432_002601"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH0641126.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1255..1818
                     /locus_tag="KR432_002602"
     CDS             1255..1818
                     /locus_tag="KR432_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641127.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2306..3142
                     /locus_tag="KR432_002603"
     CDS             2306..3142
                     /locus_tag="KR432_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH0641128.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3394..4389
                     /locus_tag="KR432_002604"
     CDS             3394..4389
                     /locus_tag="KR432_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0641129.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4646..5050)
                     /locus_tag="KR432_002605"
     CDS             complement(4646..5050)
                     /locus_tag="KR432_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH0641130.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6050..6964
                     /gene="queG"
                     /locus_tag="KR432_002606"
     CDS             6050..6964
                     /gene="queG"
                     /locus_tag="KR432_002606"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH0641131.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7070..9307
                     /locus_tag="KR432_002607"
     CDS             7070..9307
                     /locus_tag="KR432_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0641132.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9504..10556
                     /gene="nth"
                     /locus_tag="KR432_002608"
     CDS             9504..10556
                     /gene="nth"
                     /locus_tag="KR432_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH0641133.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            10750..11220
                     /gene="trmL"
                     /locus_tag="KR432_002609"
     CDS             10750..11220
                     /gene="trmL"
                     /locus_tag="KR432_002609"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH0641134.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11256..12104
                     /locus_tag="KR432_002610"
     CDS             11256..12104
                     /locus_tag="KR432_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0641135.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         5058 a         1128 c         2293 g         3848 t
ORIGIN      
        1 gttaggttag aaatttttga cgttataaga atttaaatta ctggattatt agattactaa
       61 attactagat tattaaatta ttaagctatt taaactgttt aaattgtttg aaaaatgaat
      121 tgattcttgt agaaaatagt tttaaagtat aatttttttt aacactgttt tattatagta
      181 caaacttgtg ctaaaataga atatataaca taatataaga ggtgcatatg aatacgttag
      241 atgagacatt attaaaagaa attataagtt ctaataagaa gtatactaat tatgggcaag
      301 tcgctagtta cataccagaa ttaaaaaacg ctagaagaaa tgatttagga atttgtatca
      361 tagatagcga aaataatctt tatagtgctg gaaactgtag cacaaagttt actatacaaa
      421 gtatttcaaa accaattgtg cttgctatgg cacttatgga taatgattgg gaagacgttt
      481 tttcaaacgt tggaatggag ccaagtgggg acccatttaa ttcaataatg aaacttgaaa
      541 ttaatgacac aaaaaaaccc tgtaatccaa tgataaatgc aggggctata gtaactactt
      601 cccttataaa tgggagttgt ttagaagaaa aagaagaaag aatgcttagt ttctttagaa
      661 agttagcaaa aaatgataat ataggaataa actatgatgt ctataagtct gaaaaaatga
      721 ctggagatag aaacagagct atggcttact tattgaaaag tgatggattt atcagaggga
      781 atgtagaaga tgttctagac ctttatttta aacaatgctc aattgagata gattctgtag
      841 atttagcgag aattggaata aatcttgcaa attatggagt agatatagaa aatggtgagc
      901 atttaatgag tgaaatggtt tctaggattg tcaagacatt tatgatgact tgtggaatgt
      961 atgatgcttc tggggaattt gctattaaag ttggcattcc tgcaaaatct ggagttgggg
     1021 gtggtataat ggcttctgtg ccgggacgca tggggattgg tgtttatgga cctgcgcttg
     1081 acaaaaaagg taacagcgtc gcaggagtta aagttttaga ggagttgtca aataaactca
     1141 aattaaatat tttttaaaat attaagaaag gatttttttg acaacaacat ataatttaat
     1201 atataattaa attgtaaaga aatatgaatt aaataaattt tttaggaggg gtcaatgaaa
     1261 gaggtattgg tattacgaga tttggagtgc ataaaggcta ttgcgcatcc acgtagaata
     1321 gatattctaa aagcatttga caaatcgcca ttgtcagcaa aacaattatc tcaattattg
     1381 gaagaacctc atgcaaaaat aaactaccat ataaaaatgt tatacaaagt tggtatttta
     1441 gaattagttg aggaaaaaat aaagtcgggg attgtggaaa agtactatta tccaagtgca
     1501 aaaaatgtgg ttatagggaa taggatactt aatttttctt tagacaatgg ggaagaaaaa
     1561 gaagaattgt acatatcaaa atttgaaaat atgagtgaag ttttttataa ggcagcagag
     1621 gaagatgttt tagaaaatga aaatatagtt gattatcatg atatttctct tactcatgat
     1681 gaattagttg aacttagtga cactatgaag tcaaagatag atgaaatttt aaataaaaga
     1741 caacacaatg ttgaaggttc taagtatgat atagctatgg ttatagttcc tactcttgag
     1801 gaggaatgtc ctagttagag aattaattat ggattaatta aagatataat attagagatt
     1861 gaacatcaaa atttacttaa taatcaaaaa atagtttttg gtatgggtaa aacaatgatt
     1921 tttaatctct atttttttgt tttcttatac ctattgtatt tgtaaataat atgtatagtg
     1981 taagatgtat acactatctg agaagtgctt agataatata caaagtatgt aagattataa
     2041 tatttgaaat tgattaaaat gggtataatt aaagtgtaaa tattaaaatt aaaaattaat
     2101 ataaattaaa tcaatgctag aaatatgtta aaagaggaat gagattagtt gggatagaac
     2161 atcccttaga gcttatgaat ttttagtgaa aaagtactat caaataggaa tctccatctt
     2221 catatgttgg ctaaatatag ttaaggtgga gtagttcaaa attaaaattg agtacaagtc
     2281 gtagtacaaa taaaaggaga ttaatatgat taaattagga acacatcttt caatagcaaa
     2341 aggatttgct aatgcggctg agacagctgt atatattggt gcaaatacat ttcaattttt
     2401 tagtagaaat ccaagaggag gaaatgctaa agaatttgat gaaaaagata taattaaatt
     2461 tcaagaaatt agaaaagaaa ataattttgc accactgctt gcacatgcac catatactat
     2521 gaatctgggt ggtacaaagg atgatgtata cgaatttgca acaagggtga tagatgagga
     2581 tataagaagg atggactcat taggaattga atacatgtgt tttcatcctg gaagtcatat
     2641 aggtggtggt gtagattttg gtatagatag aatagtaaaa ggattgaaca atgccataaa
     2701 gggtgatgaa aatataacta tacttcttga aactatgtcg ggaaagggaa ctgaaatagg
     2761 ttttaaattt gagcaattaa aaagtataat agacaaaatt gagcataaag aaaaaatagg
     2821 tgtgtgtctt gatacatgtc atattttttc agccggttat gatatagtga atgatttaga
     2881 tggggttttg gaagaatttg ataaggttat aggcatagaa agattgaaaa ctgtccatct
     2941 aaatgatagt atgatgccat ttggagacaa aaaagacaga catgctccta taggggaagg
     3001 aaagatagga ttaaaagcat taattgattt tatggaacat cctagtctaa aacatttacc
     3061 atttttctta gaaactcctt ttgatgatga aggtcacaag agagagctta aaatgataaa
     3121 agaaatacta tctagtaaat aaacctttat taaaaatatg tgaaatactc cgccctcaac
     3181 ataattaagg tggagataca atcatgaaat gtgataattt aaattaatgg ataggaataa
     3241 caaaaattgt atacaaatta actgggtata aagtatgatt atattatgaa tagtatgtaa
     3301 atatattgca aagattctca taatttgttg tataattaaa ggtaacatat ttatattata
     3361 taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa tactaacatg
     3421 aaaataaaga gagttggctt tgggggcatt ccaatacaaa gaattactca agatgatact
     3481 aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgacag tgcaagaggg
     3541 tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaaa taaatttttc
     3601 ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt tgaaataagt
     3661 ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt taaagaagag
     3721 gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga ggcaaaggag
     3781 caaggtaaga taaaacatat tggtataact agtcacaatt taaatactat agaaaaagct
     3841 atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga aggacaagca
     3901 gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat gaaaccttta
     3961 gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc taaagattac
     4021 atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa tgtcgctgta
     4081 cttgaaaatc ttgttctaga tgaaaaagat aataaggaaa ttgaagaaat aagaaattct
     4141 ttaggtaaaa aattctgtag aagatgtgaa tattgtatgc cttgtgctgt aggaataaat
     4201 ataccattga gttttttatg tgaaggttat tatactagat atggattaaa agagtgggct
     4261 aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg tgggctttgc
     4321 gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt tgttgaaaag
     4381 ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt tctttttatt
     4441 agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat ataaatttaa
     4501 tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt tgtttgagta
     4561 tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg agtatcaata
     4621 taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat atatcgtatt
     4681 tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata aaatcaaata
     4741 aaaatactgg cttaagttgt ttatcatttt ttattattcc acttttttca ataccatcac
     4801 aaatactttt tggataatta tgtaaatccc ccattctctt atttggaaag aataatttca
     4861 agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt ataaaacctg
     4921 caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc atccatgctt
     4981 gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct ggaattgtta
     5041 ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta tatgatttaa
     5101 aagcaaattg ttatttaaag tgtttttaca gggcttgttt gtaaaatttt ccactttaga
     5161 tgtagtaata taagataagt attttgctta caaattttaa aatttgatat atgatggaca
     5221 tattcatatt agactattat aagaccttca taatattaat aagtataggt ttagtaaaga
     5281 gagtaaaaag aaatccatat aagccaagta aagacttaat aaatggcaat gtaaatggga
     5341 tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat gtcaaaatat
     5401 aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt aagtgggtaa
     5461 aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg taaaaaagtt
     5521 aaataagtaa atgaaaaaag ctaagtatct aaatgagtta gtagataaaa aaattaaata
     5581 gataaaaaga ttaagtatct aaaagcgtta gtagataaaa aaattaaata gataaaaaga
     5641 ttaagtatct aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt
     5701 caaaagagtt agtaggtaaa ataattaaat agataaaaag actaagtatt taaaggagtt
     5761 aagcagataa aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta
     5821 aataaaaaag ttaaataagt aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa
     5881 aaaactaaat aggtaaaaag acgaagtatc taaaagagtt agtaggtaaa aaagttaaat
     5941 aaataaaaaa gacgaagtat ttaaaagagt tagtagctag aaaaggttaa aaagactaaa
     6001 aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta tggataataa
     6061 ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag caggggtaga
     6121 taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt atttaactgg
     6181 tatggaagaa ccaataattg agaacagaat taacccaaga catataatga aggatgctaa
     6241 ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga ataaaaattc
     6301 aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg actttttaca
     6361 acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt tatttgttga
     6421 taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat attttggaat
     6481 aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt atatagtaaa
     6541 taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat gtggtaagtg
     6601 tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc ccaaaaaatg
     6661 tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag ttgttttaaa
     6721 gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac ataatgttga
     6781 aatgccaaca actcatattt tggaatttaa agaaaatata atagattatt tggattatga
     6841 tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata gagcttttag
     6901 ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata agtatgatga
     6961 ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca cttgttgtgt
     7021 aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat tgaaatataa
     7081 taatacatct gaaataaatc cactagatta tatattgggt caagaaagag ctgtagaagc
     7141 tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag ctggtgaacc
     7201 tggaactggt aaaagtactt atgccttaaa ggttttaaat gaatatgcat ccaaaaaaaa
     7261 tacacataaa gattggtgtt atatatataa ttttgaaaac cctagagagc ctattatagt
     7321 tgaattagaa aaggggtttg gaagagaact taagaaagat atggagaaat taattgaaag
     7381 tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag aaaaaaatag
     7441 acttctagat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta agaaatatgg
     7501 ggaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta tccctttgaa
     7561 agaagaggaa gatgaaagtg ataagtctga tgaagagttt tacaaagcta aaagagaact
     7621 agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa tagctgaaaa
     7681 agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc atatcaagag
     7741 attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa atataaagaa
     7801 agatataata gaatatatgt atttgttcta tttagatgaa gaggagttaa aagataagta
     7861 tgataaagag cattttatta aatacaaaat taacctattt gtagacaatg aaagctcaaa
     7921 gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa atctatttgg
     7981 aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa aactattatc
     8041 aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc aattactaaa
     8101 atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag taatattgga
     8161 aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag tactgattgg
     8221 aagccactat atatatgata ttttatatag atatgatgaa gattttgaaa aatattttaa
     8281 agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag aaggaatagc
     8341 aagatttata gcataccaat gtgataagaa taatttaagg cattttacta aatcagcagt
     8401 tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac tatcaactaa
     8461 gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt ttagaaattc
     8521 ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa aaagaatcaa
     8581 tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa tagaaacaga
     8641 gggaagaaaa gtaggagtga taaatggtct gtcagtctta agtacaggag aatactcttt
     8701 tggtagagct tcaaggatta cagcgaccac tagcccagga agtaaaggca tagtaaatat
     8761 agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtgctta tattaggtgg
     8821 ctatctttct gagaattttg cacaggattt actattgtca ctaaacgcat atgtttgttt
     8881 tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat tatatgtact
     8941 attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag gttctataaa
     9001 tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag gattttattc
     9061 tatatgtaaa actaaagggt taaatggtga gcaaggcgtc ataattccta gaaaaaatca
     9121 aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa aatttaagat
     9181 atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta aatttgaaga
     9241 aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa ctgtgtcaaa
     9301 agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc aagaacttta
     9361 ataggttttt gctttttttg taaatatata taaattaaat tcttattttg tatgttttat
     9421 atattgatgt ttattttttg ttgatatgac taaactattt ataaatagtg ttttataaat
     9481 tggtatttta aagggggata attatgaaaa atgtaaatct aaaagagata aatgaaaaag
     9541 atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat gtaattgaga
     9601 aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa gaagagatag
     9661 ataaaaaaaa tgtaaagaag gttgaggttc aaaaagaaat agataaaatg attagtaaaa
     9721 aagatgagat aattgatgag aaagatatag agaattttag gtatattgaa gaagataaaa
     9781 aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa aaaacaaaga
     9841 aaagtagttc taataaggct acacaaaaac aaagcaaaga agttaaaagt aagtctaaaa
     9901 ttaaaagtga atcagccaat aaaaaagatg taaataagat tttagatgag ttggaaaaat
     9961 tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt ttaatagcaa
    10021 ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca gagttgttta
    10081 aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt agtaaagaga
    10141 taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca tctgaacagc
    10201 tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata aaattacctg
    10261 gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat ccagcaatag
    10321 ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac gagccaaatc
    10381 ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg tcacactcac
    10441 atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca gaatgtgcaa
    10501 gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg aaataacttt
    10561 tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga aaatgttaga
    10621 taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta tataatagta
    10681 taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg atacttaggg
    10741 aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca aaatacagga
    10801 aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa accactgggt
    10861 ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga tatagctgat
    10921 atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag taaatacttt
    10981 ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga aaattgtttt
    11041 atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc aaatatggat
    11101 acttgtatta gagtgcctat gttggatgtt gaaaaggcta ggtcattaaa tttatcaaat
    11161 tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa tatgaggtag
    11221 tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag ataatctgtg
    11281 acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat atagtttcat
    11341 taacagttat actggaggaa agagaataca aggatagaat tgatatatct ggagatgaat
    11401 tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct acttatgccc
    11461 aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt tatatagcag
    11521 ggtctgccac agctacaggg acgtatcaaa gtgctgtaat ggcaaagaat gatgtggatg
    11581 gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta cttgtaataa
    11641 aagcaggaga acttgcaagt gaaggaaagt ctgttgatga aatattacct gttttggatg
    11701 aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat ttacaaaagg
    11761 gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata aaaccgattc
    11821 ttgaaataag agatggacta gtttcgcaaa tatctcaagt aagaggaaag aagaatgtaa
    11881 tttctaagat gatggaattg ataaaatcaa attgtgggga tgatttatca gaccaaattg
    11941 tgtatatagg atatagtgat gattttaagg aaaaagaaca attatctaag gttatgcaag
    12001 atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata ggtgcgcatg
    12061 ctggtcctgg tgtcactggt atcatatgct taaaaaataa ataatatatt tttggatgac
    12121 attatttatt tttttattaa attaggatag aatattaatt attgagatgt taaattggat
    12181 ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga agtgtgtaaa
    12241 tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa tttcaaaatg
    12301 tacttgtaaa ttgtatataa aaaaatt
//
LOCUS       DAEPOG010000118        12244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEPOG010000118 DAEPOG010000000
VERSION     DAEPOG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.118"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..569)
                     /locus_tag="KR432_002611"
     CDS             complement(<1..569)
                     /locus_tag="KR432_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0641136.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIG"
     gene            738..2096
                     /locus_tag="KR432_002612"
     CDS             738..2096
                     /locus_tag="KR432_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641137.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            2089..2721
                     /locus_tag="KR432_002613"
     CDS             2089..2721
                     /locus_tag="KR432_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0641138.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            3050..4207
                     /locus_tag="KR432_002614"
     CDS             3050..4207
                     /locus_tag="KR432_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0641139.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KR432_002615"
     CDS             complement(4355..4990)
                     /gene="lexA"
                     /locus_tag="KR432_002615"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBH0641140.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(5094..6221)
                     /locus_tag="KR432_002616"
     CDS             complement(5094..6221)
                     /locus_tag="KR432_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0641141.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(6543..7389)
                     /locus_tag="KR432_002617"
                     /pseudo
     CDS             complement(6543..7389)
                     /locus_tag="KR432_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7419..7790)
                     /locus_tag="KR432_002618"
     CDS             complement(7419..7790)
                     /locus_tag="KR432_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0641142.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(7943..8203)
                     /locus_tag="KR432_002619"
     CDS             complement(7943..8203)
                     /locus_tag="KR432_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBH0641143.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(8392..9336)
                     /locus_tag="KR432_002620"
     CDS             complement(8392..9336)
                     /locus_tag="KR432_002620"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBH0641144.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(9357..10211)
                     /locus_tag="KR432_002621"
     CDS             complement(9357..10211)
                     /locus_tag="KR432_002621"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBH0641145.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(10226..11587)
                     /gene="accC"
                     /locus_tag="KR432_002622"
     CDS             complement(10226..11587)
                     /gene="accC"
                     /locus_tag="KR432_002622"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH0641146.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(11603..12055)
                     /gene="accB"
                     /locus_tag="KR432_002623"
     CDS             complement(11603..12055)
                     /gene="accB"
                     /locus_tag="KR432_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH0641147.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
BASE COUNT         4304 a         1853 c         1512 g         4575 t
ORIGIN      
        1 ccaatatcat gtgtaaccaa aatagtagac ttattctcat tcttaataat actataaaca
       61 tcatcacaaa ctaataatct agtctgataa tcaagagctg aaaaaggttc atctaaaagt
      121 agaatatctg gatttacaga aagtgttcta ataagcgcaa ctctttgtct catacctcca
      181 gataattctt ttggatacat acttctaaaa tcccataatc cataagtctt taaaaggctt
      241 tcaactctat ctaaagattt tttatctttt ttgccttgaa tttccaatcc aatagttata
      301 ttatccatta tatttctcca ttcaagcaaa ttatcttttt gaaacatata tccaatattt
      361 ccagtaatct ttatatcacc actagtcggt tttaaaagat tagtaagaat atttaatatt
      421 gtagatttac cacttccaga aggaccaagc aaagttaata tttctccttc tttcaagtta
      481 aaattgacat cctttaatac ttcaagctcg ccttccttag tgtagaagtt catatttata
      541 tttttaactt ctactatgtt gtcattcatt atgccacccc ctataacttt tagctcgtat
      601 atattatgtt attaacaata tgacctattt gtaccatttt tcataaatat aatataaata
      661 ttttgtttat ttacgtattt tcttagtaac ttgtacgtat aaattataat aaataaatta
      721 taaggtaggg gataaatttg agtaacataa taacgaattt aatagagtca gtatttacta
      781 atccaataac tatattgttt ttacttgcaa taactgtgta ttctgttttt aaaataatag
      841 aaaataacag agtatattca tttgtaagtt caaaattaga cgaaatcaat agagattata
      901 agacaagtga gttttataaa aaggtaaggg atgactacta tacatatagt aaagaaaatc
      961 cttatgcaga cgtaaatata acttcattta tagaggaagt tgtatctgat ttaaagcata
     1021 ataatttgcc attgttagaa aaaattagaa gtataaaaaa ttcatcttca ataagtatat
     1081 tacttggggt tttaggtacg tttgttggtc tttctacaat gcttttatgt gttgatacta
     1141 aagacattat aaattcactt ccttcaacta ttagtagtat gcaaactgca tttacaacta
     1201 gtatatttgg agttgtattt tctctaatta taggttactt tactaagata aaagattgtg
     1261 aacatgtttt gatacaaata atgttaaaat ctgaaaattt actaacatct gaaataactc
     1321 attttaagtc agaaagaatg gatttaaaag tagaagaggt aaaaaataca ataaaacaaa
     1381 taagtaagtc tatagaagct attgaaaaat ttgataaaat atctaaagat ttaaatgact
     1441 ttaatgatga atttatcagt ggtatagaag ctttaaaaag ccttttagaa ggttctcaaa
     1501 gttctattaa gacatttgac cagagcgtaa gaaaattaga taaacagttt aatattttaa
     1561 atataaaatt tgtaaagctt tttgataaat atgataatca agataatata aataaagaga
     1621 ttttatttga tataaaagaa tcttcaaaaa atatatacaa tgccactgaa agccaattca
     1681 aaattagaga ttatataaaa aatataaatg caggatttgc tctatatgaa agaagtgctc
     1741 aagatttatt aactaaatta atgacccatg aaaataaaat ctctcaaaat caaaaaatcc
     1801 ttttagatga aaagtttacc ttagatgact caataaaaaa tctctcaagt attattgaaa
     1861 acttttctaa tgacctacaa gttaaactgg atatgatgtt tgaaaattct ttagatattc
     1921 aagataagtt agatgttatg tttaataact cttttatgaa tgatgaagtt cctcttgata
     1981 gtgaggaatt atttaaggat gatgttaata atgtatttaa tccttttagt gaagaaattt
     2041 atgagataga ggataaagaa ataaaggtca taggtgagga tgagttaaat gaataataaa
     2101 tatagaagaa ctgttaacag agaatttgaa aagagtagct tttggccaac atttacagat
     2161 ttattatcta ctgtattgat ggttgtgatt cttatcttat ttagctcaga aagtatttca
     2221 ggttctgttg aacaagattt agctaagaat gtcaatgctt ctgttgaaga aacttttaaa
     2281 aaaagcaaaa tacctgtgaa ggtagataaa tctaatgggc aagtaacttt tggtgaaaaa
     2341 accatgtttg atgtagatag tgatgtttta aaaccagagg ctaaagaaat gctaaaaatg
     2401 tttgttccaa agtacataga gactatttat aaagattatg gtgactatat atccaaaata
     2461 gttatagaag gtcatacaga tgatgtggga agttatatat acaacttaga tttatcgcaa
     2521 agaagagcat atagcgttgc taaatttata gtgggagatg aaataggtga ctataaatac
     2581 aaagataaag tgactaagca tattattgca ataggaaggt caaaagctga actcatcaaa
     2641 aatggtgaca atagcgtgaa tagagatgca tctagaagag tagaactaaa atacgaaata
     2701 aatattaatc aaaataaata attaaatttt tataaaaaaa aagaaggcag ttggtgtgag
     2761 ctttaagcct tctttaagga aaaagtttat ttatttttgt tgatacttaa catattatag
     2821 tttttagaag cagtttgcaa tatattaaac gtaaatggtc gtttaaaatg cataactgga
     2881 tactactgat gtaaaatatt tcattttata taatataaaa attatacagt gtcaattttc
     2941 tatattattg tactataatc tattatttaa aatattatgt aaaaaaatat cataaaaacg
     3001 catatcatgt tataattact agagtattat gaatatggag gcgtagtttg tgatagaaat
     3061 aagtataaaa aataaaaaaa attctaaaat taaaactttg atacttttat tgatagtatc
     3121 tatatcgatt tatttaactg gttgtactac atcagataat ggaaataatc aatcaaataa
     3181 taatgattct ttgtctagta gtatatcaag tgataagaat gtaaaaattc attttattga
     3241 cactggaaat agtgatgcaa tattgattca agatggtaaa acttttacat tgatagatgg
     3301 gggagataat gatgatgagg gattaatggt agattaccta aacaatcaag gtgtaaaaga
     3361 tattaaatat cttatagcta cccattctca tgcagaccat ttaggtggac ttgattctgt
     3421 tgtcaaaaac tttaatatag aaaatgtttt tgtatcgaat ggttcagcag aaacaaagag
     3481 ttatagggac tttataaatg ctttagcaaa taagggttta tccccaagtg taccgcttga
     3541 aaataataag ttctatttag aagattctta ttttgaagtt ctaaatacaa atgggggaga
     3601 tactactaat gaacaatctc tagtccttgt atatactaat gggaatgata aagttttatt
     3661 tacaggtgat gctgaagaag gtaccgaaaa agaaatacta cctaagcttg agaaagtaga
     3721 ccttttaaaa gtagctcatc atggttctcg tagtagttca tctcaagagt ttttagataa
     3781 agtaaatcct gaatatgcgg ttcttttagt aggcaagggt aattcatatg gtcatccaca
     3841 tcaggagaca atgaataaat tgaaaaaaat gggagtaaaa gtacatagaa gtgatgagtg
     3901 ttcagatata atatttgaat caacaggtga tggagtattt acaagttgta aagatggaag
     3961 ttataataaa ggtgttagag aagatggaaa ctctggaaaa tcaaacagta atacaagtaa
     4021 aaatgattct tttaaaaata aacaaaatac attcaattca gaagaaatag tgtattttac
     4081 tccaaaaggt aagagttatc actctataaa aaattgtagt gggctttcaa gaagtaaaaa
     4141 aatattatca ggcacaattg aagaaagtaa gaaaaatgac ccatgtgata tatgttatgg
     4201 aaaataacaa aaaaacaacc aatttatcta taaagcttct gaactttaaa tcatttaaaa
     4261 aataaaataa atgcataaaa aaaataagct ataatcaata gataatacat ttcatctata
     4321 cgaatagctt atttttttaa gtatttttta atagctattt aataactcta aatacaccag
     4381 ttacaatacc tacaatttca acttctgagt cattgagcat aattggttcc ataaaatcat
     4441 tttctggctg aagtcttatt aaatttcctt ctttaaaaaa cctcttaact gtagcagatt
     4501 caccatttat aagtgcaact actatctgag aatttaaggc cgtatttttt ttgtctacta
     4561 caacgtaatc ttcatctaaa attcctgcat taatcatact ttcgcctttt actcttaata
     4621 caaaattgtt actgcccttt actaaactgg cagggaatgg aatgtattcc tctatgtttt
     4681 gttgagctaa aatgggttct ccagctgtta tttgacctac taaaggaagc tctattattt
     4741 cttgattagc tccagaaaca tcatttactt tactacgttc taaaacttca atagctcttg
     4801 gtttagtagg gtcctttctt atgtatccca gtttttcaag tttatttaga tgagagtgca
     4861 cagttgaagt agatcttaac cctacagcag tacatatttc tcttacagca ggtggataac
     4921 cttttaatgt gatttgagac tttataaatt ccaatatcaa gacttgcttt tcagttagat
     4981 ctaaatacat aacatcactc atttccaaac atattttgtt tttatttcca tacactttat
     5041 atttttaatt ataacataat ccatttatat ctgcaaacat ttgttcctga tttttaatcg
     5101 tcctcaatcc attcaaaatt cttgactata tcacgtttta catttttctt atgagctgca
     5161 taaagctgag tagttgtgac attatcatgg tcaagtaagt tttgaacatc atatatagaa
     5221 aatcctgttt ggattagaga agttgcagcc gttgctctta atttatgggg actatatcca
     5281 ttgtctctag tagtatcaag tggaatagat gtatattttt ttaccagtgt tcttatagcc
     5341 tttgctgtaa ttctcttatt ttgaagagac aaaaataaag catctttttc ttcatcattt
     5401 aatagttcat ctcttgttct ttcgttttgc aaataatctt ttattacatg ttcacaagta
     5461 tgatttatag gcattaaaac ttctttccca cgttttctat atattttaaa ttcacctctt
     5521 gaaaaattaa aagaggagat attgagttct ctaagctcat ttaatctaag cccgtatgta
     5581 acaaataatg ctagtatagc tttgtcacgt aatttagttt ttctccaata taccttttct
     5641 ttctctgtaa gcccttcacc tgtttctact gattcaagca tcttagcaac ttcatctatc
     5701 tcaaggcgct taatagcatc tggctgaggt ttaggaagtt ttattggatt gaatccatca
     5761 gttatattat tatctatctg agagtttctg tataagaatt taaatagagt agagatggat
     5821 gatttttttc ttgctaacgc tctattgtta ttttcaaaaa tcaatgtgtt tttttctgta
     5881 tttttgtaat atctactgca ataatcacct aagaataaat ttatatctct tgattttatt
     5941 gtattaaaat catcttctgt aatatctttt acacaatctg catttgttaa ttctttggtt
     6001 tcaatcaaat atgaacaaaa aaaattaata tcttctaaat aagcaagtcg tgtagaaact
     6061 gcaacagaac cttttaaata tataaaataa tccttcataa atacaggaag tttattttcc
     6121 aaaagtagac atttttctat gagttcattt tctttgataa caatattatc aggtttatct
     6181 gatttattat gtacttttat aattttctta tcattattca aaattaaacc tcctaaaact
     6241 aaaatataaa tcttttatat tattttttga tatttatacc aagtgagtat aaatatttaa
     6301 tttaatcatt ttttacattt ttaagagatt agcagtgctt tagatgaaat tatcactaac
     6361 tattccctaa atccacattt aaagactagt tgaatttaag tactcttttt ttatttctca
     6421 atatatttat agaaatattt taaaatatat tgagaaattt tttatgctct actaaaatct
     6481 tttttatata atatttttat atatttctaa cgaaaccaaa tattagtaaa atatgtattt
     6541 atttaactaa agtttttaaa tgggctgact aagcttaaaa tataggatat tatgagagtt
     6601 actattgaaa aaataaaccc tattgccata aagaaactta taaatatgtt atacttaact
     6661 attaaactgg atatattcaa gttatcaaat ccaaataagt atgtaattga acttattaca
     6721 aaaataacta taactataaa gctcagacca tttaagctat tttttaaatc agcactacta
     6781 agtgacatat gtgtagatat agaatacatt aaaaacataa atataaaata cttcaatgaa
     6841 tatacactag gtgttaacaa cattattctt ataaaattgg aaatatcacc taaaataatg
     6901 tgtataaaac catttatatt taaattatta atcatactta catataactt taaatctata
     6961 ttttgagtca ctttcatata actatctggc agtaatatct taaaaattaa aataatcatt
     7021 attgttccac ctataatagg tgcaattcct ataaagaaat ttcctatact ttgataaaga
     7081 ttatcttttt tatagctatg acttacatat cctagtatac catcattttt actttctatt
     7141 ggtctgaaaa acttaactga atttatttta tgcatgaaaa tcaagcacat tatcaaatga
     7201 ctcaattcat gaacaaacgt tcctatagct ccagttacaa taattccagt ttttccaaaa
     7261 gatgaaaaga taaatttatt atttagattt tctgtaatgc taaaaatcaa tccaaataca
     7321 ataaatattc ctatgaaaca aaaaaatatt tattacactt atttttaaaa tttcgattat
     7381 ataaatcata agttaagtcc tcatgtaaaa gtatttaact actctacttc atatccatta
     7441 cagcttaaaa ttttacaagc atcttctata tctttttctt ttactaagat atagtctgta
     7501 tcatacgtag aaattgcaaa tatgcttatt ttgttgtctg ctaacaaact acttaactta
     7561 gataaaattc ctacaagtga aaagtctaaa ggacctagta tcttaagtat tctccactcc
     7621 ttttctgact taacatcaga aggaatatta ttatttaaac atacaacact taattcatca
     7681 tcagttctag ttatagagta aaacttctct gtatctatcc atgttggtat tttactgtca
     7741 ttatttaatc tacatactgc atactcttct accaataatt ttaatttcat aatgtatatt
     7801 tcctccaatc ttatttgttt acttaagatt atacattaaa agttacataa aataaaacat
     7861 ataaagtaaa aaaggttcac tatagtgtcc tatttctata gccaaccttt aaaacttaat
     7921 taaattctaa taaagagata tattatctag gttcaattat aagtttaatg gctgttttct
     7981 tatcaccttc gatttctata tcagtaaatg cgggaatgca aactaaatcc attccactag
     8041 gtgcaacaaa acctcttgct attgcaattg attttatcgc ttgatttaga gctccagctc
     8101 ctatagcttg tatttctgca acgcctcgtt ctcttagtac tccagccaag gctccagcta
     8161 cagaattagg attagatttt gatgaaactt ttagtacctc catttcaaca tctccttgtt
     8221 aatttgttaa agtatccatt acctaaagta ctaaatcata attgccattt actaattaca
     8281 atcatcattt aaagtatact tttataaaaa tatacttcta taatcgtttt aagaaaacct
     8341 ttattttttt taaccatttt attatgaaaa aactacaaat ttctttatat tctataagca
     8401 ttttccaata tttctaaatt ttttatatct ttgagctata atttcctctt tatccatatc
     8461 tttgtaatga tttaatcttt ctaaaatata agcttttatt tcttttgaaa cagcttctac
     8521 atctttttgt gctcctccaa gaggttcttt tataatctta tctattattt ttaaatcata
     8581 taaatcatta cttgtaagct tcataacatc acaagcttct ttagctttag atgaatcttt
     8641 aaatagaata ctagaaagcc cttctggaga aatcacagaa taaactgaat gttctagcat
     8701 acaaacatca tttcctactc cgatacctaa agctcctcca ctaccacctt caccaattac
     8761 aaaagtgatt actggaacag aaagtttact catttcaagt aagtttcttg caatagcttc
     8821 tccttgtcct ctttcttctg cttctaaacc acaaaaagct cctgatgtat caataaacgt
     8881 tattataggt ctattaaatt tttcagcttg tttcataagt cttaatgctt ttctgtatcc
     8941 ttctggatga ggcattccaa aatttctttt tatattttct tttgtatctc ttcctttttg
     9001 gtgtccaact atagtaacat taaactttcc tatctgacca atccctccaa ccatagaagg
     9061 gtcatcttta aaaagcctat ctccatgaag ttctaaaaaa ttagaacaaa tatgttctat
     9121 gtattccaat gtcgtaggtc tctttatatc tctacttagt gttacctttt catatgctga
     9181 tagatgtgaa aaagcatctt ccttaagctt ttccaatttc tcattaagtg aatttatttt
     9241 atcacttaaa tctatattat ttgccttaga aatagttatt aattgtttaa tatcattttc
     9301 taagactttt attttttcac tattattttc aatcaaaaca gttcctcctt tatgccctat
     9361 ttcttgtgca tagcaagaat ttgatataat aattccttca tttttcttct atctactatc
     9421 atgtcaataa aacctttttc aagtaaaaac tcagaagttt gaaatccttc aggaagtttt
     9481 tgatttattg tttgctctat tactcttgga cctgcaaaac ctatcaacgt atttggctca
     9541 gaaattataa tatcaccaag catagcaaaa ctagcagtta ctcctccagt agtagggtca
     9601 gttagaactg atatatatgg caatgatttt tcttcaagct tagaaagtgc ttgagaagtc
     9661 tttgccatct gcataagaga taccatacct tcttgcattc tagctcctcc tgaagcagaa
     9721 caaataatta taggtaaatt attttcagca gcatactcta ttgaataagt tattttttcc
     9781 ccaacaatag aacccatact tcccatcata aaatcaggat tcattacaca aataactgtt
     9841 ttaattccat ttattcttcc ataacctgtg acaacagctt ctgactcttt tgttttttcc
     9901 ttataagagt ctactttttc tttgtattta ggaaaattta gtggattttg aaattctaca
     9961 tcagaattaa actccataaa agttccttta tcaatcaata aatttattct ttttcttgct
    10021 cttaagccat aatgtcttga acattttgga catacaaatg aattttcttc tatgtccttt
    10081 ctaaatacat gactatcaca acctttgcaa taggtccaaa atttatcatc tacactattc
    10141 tttttaaagt tgtcatctaa agaaatcgta acatacttag attcttttga tgataaaaat
    10201 ttttttatca taatctattt actccttata atgtagcaaa ttctttcttc ataaagacat
    10261 cttctataaa tttggtatga tgagtatctt ctctaaactc ctcacttgtt acaagtgcct
    10321 tttgaaattc tacatttgta ttaatacctt ctacaataat ttcgtctaga gctctatata
    10381 ttctacttat acattcttct ctagtttcag cccaacatat taacttacct atcatagaat
    10441 catataaagg tggtatagta tatcctgcat atacaaaagt atcaaatcta acaccaaaac
    10501 ctccaggtaa atttaatgat tctatcttac ctggtgatgg agcaaaattc tttgatgaat
    10561 cttctgcatt tattctacat tcaatagcat gacctctaaa agtaatatct tcttgtttaa
    10621 aaggaatttt atccccatta gctatagata tttgaagttt tataaggtca agatttgtta
    10681 ccatctctgt aactggatgt tcaacttgta ttcttgtatt catctctata aaataaaagt
    10741 ttttatcttt gtcaactata aactcaatag tacctgcacc gatataacct actcctttta
    10801 ctatatctac tgcaatttta tgaagttttt gtctttcttc atctgaaagg tatggactta
    10861 aagattcttc tattactttt tgatttcgcc tttgcataga acaatctcta tcacctaaat
    10921 gtattgcatt tgaatgttta tctccaaata cttgtacttc tatatgtcta ggattttcta
    10981 taaacttttc catatataaa tcactattag aaaatgctgc tagagcttct gaacttgcca
    11041 tatcaaaatt tgaaaaaagt tcttcttctt ttctaacgat tctcatacct tttccaccgc
    11101 ctccacttga ggcttttatc ataacaggat atccaatttc tcttgcaact tctaaagctt
    11161 cttccttagt tttcaaaaca gattctgaac caggaacaac agggatattc aattccatca
    11221 tagtttctct tgctctagat ttatctccca tcaaaatcat ttgttctctt gttggaccta
    11281 taaaaataat attattatct tcacattgct ttgcaaattc aggattctct gataagaatc
    11341 caaagcctgg atgaatagca tcacacttta atttaagagc aagatagatt atattttcta
    11401 tattattata actcttactt atattatttg gtcctataca aatagattca tcagctatat
    11461 atctatgaaa acaatcttta tctatttcag aatagatagc aacagttttt atacctagtt
    11521 ctttacatgt tctgatgatt ctaactgcta tatcgcctct atttgcaact agtatttttt
    11581 ttatcaactt aaatacctcc tactacaatg gttttatctt aaataaaggt tgattatatt
    11641 ctactagttc ttcattacta actaatactt caattatttc acctttaatt tcgcttgtta
    11701 tctcattcat aagcttcatt gcttctaata tacataaagt gtcgccttct tcaactacat
    11761 cgccaacttt aacaaagcta tctgcatctg gacttggaga atcatagaat gttcccatca
    11821 aaggagctac tactactgat agattctcat tagatgtgac aggtgtatcg tttacaactg
    11881 gcttttctaa agaaagatcc actactgatt cttgttgtac acttaaaaca ggtgaacttg
    11941 tagattgagc ttttttagat acttctaatc ttaaatcgct actttcaagt tttacatatt
    12001 ctaagttagt tgagtctatg gcctttaata attccttaat ttcattgatg ttcattttgt
    12061 tacccccttg tattattacc tggtgctaat attatatcac aaaaaacaaa tttataaaag
    12121 aaaaagtgct gactagagaa tcaacacatc aatatatctt ttaattttaa attaacaacc
    12181 agtaacttat atttgtttta attttccatt attaatataa attataagta taaaatgaat
    12241 aaaa
//
LOCUS       DAEPOG010000119        12106 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEPOG010000119 DAEPOG010000000
VERSION     DAEPOG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.119"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(334..1457)
                     /locus_tag="KR432_002624"
                     /pseudo
     CDS             complement(334..1457)
                     /locus_tag="KR432_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            1654..1878
                     /locus_tag="KR432_002625"
     CDS             1654..1878
                     /locus_tag="KR432_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641148.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            1931..2455
                     /locus_tag="KR432_002626"
     CDS             1931..2455
                     /locus_tag="KR432_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641149.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(2560..2706)
                     /locus_tag="KR432_002627"
     CDS             complement(2560..2706)
                     /locus_tag="KR432_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641150.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(2845..3771)
                     /locus_tag="KR432_002628"
     CDS             complement(2845..3771)
                     /locus_tag="KR432_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0641151.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            3962..5833
                     /locus_tag="KR432_002629"
     CDS             3962..5833
                     /locus_tag="KR432_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH0641152.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(5925..6860)
                     /locus_tag="KR432_002630"
     CDS             complement(5925..6860)
                     /locus_tag="KR432_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBH0641153.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            7380..7892
                     /locus_tag="KR432_002631"
     CDS             7380..7892
                     /locus_tag="KR432_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH0641154.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(8047..9108)
                     /locus_tag="KR432_002632"
     CDS             complement(8047..9108)
                     /locus_tag="KR432_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBH0641155.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(9232..10125)
                     /locus_tag="KR432_002633"
     CDS             complement(9232..10125)
                     /locus_tag="KR432_002633"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH0641156.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(10135..11616)
                     /locus_tag="KR432_002634"
     CDS             complement(10135..11616)
                     /locus_tag="KR432_002634"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH0641157.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(11724..11955)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4573 a         1530 c         1698 g         4305 t
ORIGIN      
        1 tgtcaagttt tgatacatgc ttttttattt tgaatatatg ttttctataa atttatgatt
       61 ttttagttat gtaattttat atgtgaattt ttagattttt atatagatag tgaaataatg
      121 tggaagtttt atgttttaaa aattttagtt aaaacattta ttaaaaaaga taatagaaat
      181 gttttaacta aaatagttag aattgaaatg ttatacagat agtataagta ctatcaaatt
      241 atataagatt gcttgaagtt aaataattta tttaaataga aaacagctga tttaatatca
      301 gctgtatatg aataaaattt tgaattgtag tgtttagtta tatggatata attcataata
      361 agttagcaat aaatcaacca ttttaccata actctgagta ccttcagtaa cgccattagc
      421 ttttaagtaa gaattgttaa actcattaga aatttcatta attttacctt catatttttg
      481 ccaaaattca ctttcatttt ttaaatctct acggacagaa tctgaaatgc cagcagaaat
      541 atcaacatat gcattataat caaccttact taaagcagaa gcagtataat ttaaagctaa
      601 aatatatcca gaatagttaa aatcaatatg ggggtgtttt atagaagtaa gatacgctat
      661 aaaattagcc tcatcttcac tagcaaatcc cctttgatgt gctatttcat gttcaactgt
      721 acaaggaata tataagtcag gtattgctat atttacattt gcctcacctg taaaaggaaa
      781 gtatatacca gttattcctg tatagcacat tagatttgat gaaataatat acttaggctt
      841 agaataacta ccactaacac taggtaatat atccaagata ttatcgtatc ctagttgagc
      901 tctatttatg acaccattat aatctgtatt agattttaca ataccatttt tatcttgtaa
      961 tgtgagtttt ctagtttcat ttgctttaat tacaaggaat ttatataatt tagtaagctc
     1021 tttaacacta tattgttttg attggataga ggtattgttt tttaaattat agttatttat
     1081 aagtgtggtt tctagtggaa tcctattata gtttattccc caaagcacta caaaaagaaa
     1141 gtagactatt gatacaatgg ataatatatt taaaatggaa ttttttaaaa atatgtttaa
     1201 ttttttttct tattaaaaat tgtagataaa gtacaacata aaaataagaa gatagatata
     1261 actattatgt acattgtaat ctcatagaga gaaaatggga aaattccaga aactttactc
     1321 aatatttgta caattatttt atctatgaat tgggagtaat atttttcaac aatatttgga
     1381 actttgctgg ctataaaatt taaaagtaaa gcaataggaa agagaattaa agaaaaatat
     1441 tttgtttttt tgctcatatt tcacctcaca tacattagtt tatataaaca tattatatat
     1501 caattgaaga aagattgtaa aataagtata aatatatcta tttataactt tatactaaat
     1561 aaatgataag ttataatata ttaatatgaa taagcagtat atatacaact aataataaga
     1621 aaaatatcaa ttatataagg agtagataaa aatatgataa taaaacttac taaagacaat
     1681 tttaatttta gatttaaaca atatgaaggt atgttttttc atgctgaagt agttgaaggt
     1741 atggaatcca ttagatataa aataactaag gataaagaag gtacagaatt aaaagaattt
     1801 ggaagtacac atataaaaga taatatgata tatgtacctc aggtagaagc atacatagat
     1861 ttgaacagta taaaataaag atgtaaaatt ttaattaaaa ataaagatta tgtttgaggg
     1921 ggaatccaat atgagtaagc ctatagatat aacaataaga cctataagaa gagaagatgc
     1981 acaagctttt aatgaaatta gaagaacata cagtgttatg agaaatactt tggcattaat
     2041 gagtgacaga gtcgataaaa ctgaagctat gttagcttct ttgggagaaa atgattatat
     2101 gtttgtagct gaaactaata ataatggaga aaaagatgta ataggttttg ctgggttaca
     2161 tgtaaattct tctccacgat tgagacatag tgcagaaata ggaataagtg ttgcagagaa
     2221 ttatcaggga agaggagtag gtaagaagct tatggaacaa cttttagata tagcagataa
     2281 ttggattatg ttaactagag taggattaga agtaattgta gataatgaaa agggattaaa
     2341 tttatataaa aaactaggtt ttgaaataga gggaactaaa aaatatgctg taatcagaga
     2401 tggaaaattt gaagatgttt atattatggg aagatataat aaaaatttaa tttaaagaag
     2461 ctaatagaga agtaaaatag agtgtcttta ataaaaaatt tacattaaaa ataatctatt
     2521 ttacttctct attgtattaa aaaacttatt tttaaatgtt tacaaaaaca ttctatatac
     2581 aaataaaatt attagcaata tcaacgctat tttaaaagct aaactagcaa cagttttaat
     2641 gatttttaca gaaacaccta tagcaactat agcaaatact attttaaaga gtaaatccat
     2701 atccatattt aagcctccta ttctataata catatagata ttattgacaa atatctagga
     2761 aaaaattcca tcaatatact acaataaaat gtttatattt gtaatttatt gaatatgtta
     2821 ttatataata cataaaaaaa taaatcaaga taagagtaaa atacttataa aaggtataga
     2881 tataactgaa aatagtgttg taaaaaacac acattgtgaa gctagtgtta tattggagtc
     2941 atattgatta gccattatag cagtatttgc agcagcaggc attgaagaaa ttactactgg
     3001 tatagctaaa atcatcttat catttatcca tccttttaga ataaaataca caatcacagg
     3061 aagtaccaat aatcttataa aagtgactat atataatttt ttgttgacaa aacaatcgag
     3121 agctgaagaa ttagcaagta agcttccaat gataatcatg gatattggtg tagttatact
     3181 tcccaacatc tcaagtggct cgtttataaa ttggggtaac ctaagtcctg ttacaaataa
     3241 aaaaagtcca ataacaatag ctattgtagc agggcttata agtgatttta ttgaaaaacc
     3301 tctacttgta ctgcctttag ataaaagata aattcctaaa gtgaaagaaa ataagttaaa
     3361 aggcaaattg aatattgcag tatagaatat tgcttcatga cctaaaactg cttcaattac
     3421 aggataaccc ataaaagcaa catttgaaaa aacaactata tactgatata ttcccaaatc
     3481 ttttttatca ttacatttta aaatgtattt caataaaaat gcaattataa ttgaaactat
     3541 atacataatc aaagatgtaa ataataacaa tagtattttt tgtatcattt tactatcaaa
     3601 gttaatttgc atggagctaa ttatcattga tggtaaaaaa actgtcattg tcaaagttga
     3661 aagcttagat gtgcagtggt catctaataa gttgaatttt ctgacaaaat atccaactaa
     3721 aataattata aataaaactg caacttggat aaaaatattg ctgaaattca taaaatgtcc
     3781 ccccctatat atacattata taccaattac ataataaaat ctaatgatta taaaatttaa
     3841 aatttaatgt tacaatatag tgacaaaagg taaaaattca atattatagc attaattata
     3901 gattataatg tataataatg tttgagagaa tatattttat aaaatgggta ggtagcgaat
     3961 aatgaaaaat acaaaatttt ctataaaaaa aataaaaaat atatctaatg acccgaaagt
     4021 cgtacatact aagggtgccc tccttgaaaa gatgggtaag atggaaagcg caatagagtt
     4081 gtataagagt gctgcattgg ataattacgt aaaatctcaa tatactttag gaaatatata
     4141 tgaaagtaaa aaacaattta aagaagcaga aaagtggtat tcaatggcat ataaaagtgg
     4201 aagtgaagat gctgcttttg atttaggtaa tatgtactat aagttagatg gctatgaata
     4261 tgctatatat tggtatgaaa agatagcaaa tttaggttat ttacaagcac aaaataattt
     4321 aggtgtatgc cattttaaaa tgaaagattt tgtaaggtgt gaaaaatggt taaaaacagc
     4381 agcagacaag aatttaggta aagcttgttt taatttaggg gtattatata cttttttgga
     4441 gaaagatgat gaagcatatg aatactataa aaaaggttct gtgcttttag atgatgatgc
     4501 aaaatataat ttagcaatat taaatggaaa aaagaaagat aataaatcaa cagtaagttt
     4561 atataaacag ttgtacaagt caggtcatat gaaaggttgc tttaacttgg gtatgattat
     4621 ggaaataaat gataatttag aagatgcaga gaagtattat aaaaagagtg ctgatagaga
     4681 tgatgtgaag tctgagtata ggcttgcata tgtatatgat agaaaagaag agctgggaga
     4741 tgcaatttat tattatgaga aagcaataga aaaaaatcat acactttcta agtatagact
     4801 tgctaattta tataatagag aaaatgagat agagaaggca aaaacttatt ataaaatggc
     4861 tgctgaagat gatataacag aagcaaaaaa taatctggca ggtatatatt ttgaagaaaa
     4921 agattatgaa aatgctatta aatattatga ggatgctata gctgttggat gcaaaagttc
     4981 ccttgaaaat cttggggatt tatattatca gaatcaagat atagaaaaag cgatttcgta
     5041 ttattcgagg attccaaata atgcatcttg tcaaatcaaa ttaggaaata tctatgaaga
     5101 tttaaataat attgaagaag ccataagttg gtataagaaa gcatctgaaa atggagatac
     5161 gaggtctagt tatagattgg ggtgtatata tgagagctta ggaaatacaa aaaatgctag
     5221 aaagtatttt gaaatggcaa gtagcaaaaa tcatatgaat gctcgtatac atctaggtag
     5281 aatttatttt agagaaggta aattggaaga gtctaaaatg atgtttgata cacctgccaa
     5341 tgaaaataat gtgtacgctc aacatatggt aggattaatt tatgatatgt tctataaaga
     5401 ttatgtaaat tctaaatttt ggtatgaaaa ggctagagca caaggttgtg ttgaatcaat
     5461 atataattta ggccaaattt atttaaaatt aaatgatgac gcagaagctg agaaatatta
     5521 taaagaaggt attaaatatg gaagtaagaa gtgtgagtat atgcttgcag gtctttatta
     5581 taaaaagagc ctagatatgt acatttcttt agcaaatgaa gagtacgata attgtgaaga
     5641 gatagtagaa ggctttccca atttaaatat agactttgat gaagtattaa ttcctccatt
     5701 taagttgcaa gaagaggtaa tagatgaaga agagtatgta ccaatttata tactaaatat
     5761 aaaagaaagt ttagattcaa tgctagaagg gcttgaaact gaaatgatta tagatgaaga
     5821 acatgatagt taaaaagtat tataagatta tatagattta aatatataaa agccattagg
     5881 ataaatgtaa atcaattaat tctaatggct tttttattaa tattttataa tagcttattt
     5941 cttgacatga aaaatgtaat cactatacat attatagagg taataagaag tattatccaa
     6001 aatccgtttg ggttattaga aaatggcatg ttgatgacat tcataccaaa gaatcctgat
     6061 attattgtag gtattgagaa aattagtgta actactgtta aaatttgcat aacattattc
     6121 tgattattac ttataattgc actgaaagca tccataattc tactcaatat atctccatat
     6181 attttagcca tatcaagagc ctgtctattt tcaactataa catcttctaa taggtcttca
     6241 tcatctggat acttctttat accagcagtt ctaaccattt tgtttaaaac aagttcatta
     6301 gattttagtg aggtagagaa gtataccaaa gatttttcta attccaataa ttgaaccaat
     6361 tctttgtttt tcattgattt gtaaatttct cgctctatta tgattgtcat tttatttatt
     6421 tttcttaagt aatgcaagta ataagctgca tttttgtgaa gaatttgaag caaaaagcga
     6481 gtctttttaa acgtaaaaaa atctttaata tgaccaacta taaagtcatt taaaatctta
     6541 gtctgtgcat cacaaactgt tacaatagct tcttcagtta gtataatacc taagggtatt
     6601 gtagtataat gagaagaatt agagtcactt tcgtctactg gtatatctat aagtataaga
     6661 gtatgattgt cttcaacatc tattcttgag cgttcttctt catctagtgc agattctatg
     6721 ctttctacat ctatatttaa taaattagaa atttcgttga tttcactatg attaggttca
     6781 acaagattta tccaacagcc atcttcaaaa aaactaagtt tctctaattt tgaatctata
     6841 gttttgtaat atcttatcat ggcaacacat cctttattca gttttatata aatctcatca
     6901 tttaatagta tctattaatt aactaattta tataactgaa tatatgtatt agttttgatt
     6961 ttagatatta ttaagtgatg aaaatgtaaa aaattctctc cgcaacggac tagtatccat
     7021 tgcccatcac tctccttatt tattatttta tatatagcta tttatgaaat gcgtatttta
     7081 agcactcttt aatataatac cttttccagg tatataaatc aagttttgat ttgattaaat
     7141 aatattttta ccataattta cacataaagt atataagtta tatttaattt gagtaaagaa
     7201 tatatatatt ctgagtgaaa ttattaaata tattaaaaaa taagagtgta tatatatatt
     7261 tacttatttg caataatata tttaaaaaag tattttattt tcaaaactag acttcattta
     7321 taatttatca cgaaaataaa aggaatataa atataagaga atgagaggtt attagagata
     7381 tgagtgattt taaagaaatt ttggcagaag agattaaaaa taatccattt ggattgatag
     7441 gtaaagattg gacacttata acagcagaaa atgaaggtaa agtgaataca atgactgcta
     7501 gctggggagg acttggagtt atgtggggaa aagatgttgc ttttgtagta ataagaccac
     7561 agcgttatac taaggaattt atagataata cagataaatt ttctcttaca ttttttgatg
     7621 aagattttcg taaagagctt tcatattgtg gaaaggtatc tggtagagaa gaggataaga
     7681 tatcacagat tggatttaat atagaacact taaatgatac accttatttt aaggaagcta
     7741 aaatggctat tatatgcaaa aaaatttata gtcagaaact tgaaccccaa tgttttattg
     7801 ctgaaggtat agatggaaga tggtatccac aaaaagatta tcatacttta tatatagctg
     7861 aaataaccaa tgtacttgta aaagaagatt agttataagg gttaatacta tctctaatta
     7921 aattaaaatt tcaataaaag gaaattgaca caaaaaagct aaggacataa atatgtttat
     7981 aaagattaat aaataacgta ttaaaactcc ttagcttgat aaaattttaa tttagttttc
     8041 tttaaattat aattcagaaa tcaattcatt gataacatct tcaacagtac ttaatttatc
     8101 aattttatag gcatcatttc cacaaaataa aagtgaattt tcaacatctc ctttaactgc
     8161 attgattaga gcttgagaaa tacagtaagg agtttctttt ggattacaag gaatcaaaca
     8221 attatagcac ttctttattt ctggacgatt tattttaact tcttctataa atttgtttct
     8281 tatggcgcgt ccaggaagac ctacaggact tttaactata tgaacatctt cttcactagc
     8341 atttatgtat gccattttaa agttctcatg ggcatcacat tcgtaagtag caacaaatct
     8401 tgtaccaact tgtacaccac ttgcacccat atctatatat tttttaacat cactactaga
     8461 agttatacca ccagcagcaa ctactggtat gtttctacca aatttttctc cataagtatt
     8521 tgcaaccttt aaaatttcaa caaattcctt atcatagtct atagaatcta tgttatcaag
     8581 ttcctcgtta gagtatccaa ggtgaccacc agcttttgga ccttcaacaa ctattaagtc
     8641 agcagtagtt ttctcttttc tatcccacat ttttaaaatt acattagcag cttttgcagt
     8701 tgaaactatg ggtgctattt ttacattact tccctttact aatgatggta gcttattagg
     8761 taatccagct ccagatatta taaggtctac accagcttta acagcttcct ttacgtatgt
     8821 ttcatattct ttcatagcta ccataaaatt aataccaata ataccaccat ttgagatttc
     8881 tttggcctta gaaatatgtt tttttatagc tcttaaattt gcatttatag tattagtttc
     8941 aaaatcttcc tcatcatatc caatttgaac tccagaaatt acaccaacac caccaagctt
     9001 agcaactgca ccagctagtg atgacctaga tataccaacg cccattccac cttgaattat
     9061 aggaactttt gcaactaaat ctctaattat taactctttt atgataatcc ctcctcaatg
     9121 taaacatgtt taaagaacta gattataata cttaatatta ttacctagtc ctaattatta
     9181 tactacaaat tataataaaa gagtagttat attttgtata gaagacgata attatattat
     9241 atttaaaaca gctccattag aatcgcaaca taactctttt atttcccaat cagattttag
     9301 tttttgtagg taatttttca tattatccac aaaactatca tcattttctt ttattaaaga
     9361 cattatagta ggtccagctc cacttaaaaa tactccaata ctatttaatt gttcagattt
     9421 ttcaacaata tcattgtagt tctcaattag agttcctcta taattttgat gcaatttgtc
     9481 ttgacaagct actttaagta ggtcaaagtt tttgtttagt aaggcagata ttagtaaagc
     9541 aactctacta acattgaata ccccatcaga atatggtatt tcctttggta aaacacctct
     9601 agctttttct gttgataact taaaattagg tatcattgca caaaatttaa gttcttcagg
     9661 aatttttata atatcataat gtatattctc attatcagta acagatacga tcatatttcc
     9721 taaaatagca ggagcaacat tatctgggtg accttctatt tctgaagcta tatttaataa
     9781 ttgattttta tctaaattag cacctgatag agcatttgca gctataactc cagctactat
     9841 acaagaggca ctactgccaa gacctctaca tataggtact tcactgtgga tttttatttt
     9901 gataccagca ggtatttttt ctggtttaac tctatcaaaa aagtacttca tagatgtata
     9961 aactaagtta ttttcgtttt tgtaagcatc ttcacaacct tctatttcaa gaccactttc
    10021 aatttcttca acataaaatt tattgtatat atttaacgca attccaagac aatcaaatcc
    10081 tggacctata ttagcacttg ttgcaggaac tataatctct aacataataa tcacctatac
    10141 ctttaagaat ttttttatag ctagttttaa ttcatctttt tcataaatat ctttatgtag
    10201 tatttctgca tttttaagat ttttaatagg ttttggaata tctattttag aaatacttga
    10261 taatctatca attatagcaa agtcatcaag gtctttaaaa tcacaatcta tagactttag
    10321 aacatcttca gaaaacttga atggacttgc agtacttaca ataacagttt ttgtgttatc
    10381 agaagtttct tttttatatt tttcataaca attgtatgca acagcagtat gagtatctat
    10441 caaataatta taatttttaa acacattgtt tatcgtgctt ttaacttctt cttcatttga
    10501 atattctcca tagaatgagg ctaaattttt ttccatatca tcacttattt tgtagactcc
    10561 tttatcacta aggtctgata acagcttatt tacaatatca gtatttctat ttgatatttc
    10621 aaacagtaat cgttctaaat tacttgaaat taatatatcc atagaaggag aagtagttaa
    10681 tttcaatgtt ctatttttat catatacacc agttttaaag aaatcataaa gaacattatt
    10741 atcatttgat gcacatatca atttgtttat tggaagaccc atttgtttgg cataatatcc
    10801 agctagaata ttaccaaaat taccagttgg gacaacaaaa tttattttat catttaaatt
    10861 tatctcacta ttgctaacta atttcatata tgaataaaaa tagtatgcaa cttgtggaag
    10921 aagtcttcct atattaattg aatttgcaga agataatata taattattat ccaatagttt
    10981 ttgattaaat tttttatcag aaaaaatctt ctttacacct gattgtgcat catcaaaatt
    11041 tccttttata ccaactacat atgtattttt tccagtttga gttttcattt gaagtttttg
    11101 tactgggctt acaccatctt ctgggaagaa tacaattatc tttattttat ctatatcttt
    11161 aaatccctca agtgcagctt tgcctgtatc tccagatgta gcagtaagaa tcacaacatc
    11221 tttctctaaa ttatctttct ttatagatgt tttaagaaga tgaggcatta tagtaagtgc
    11281 catatcttta aatgctaaag ttggaccgtg atatagttct aagaaataag tatcatttac
    11341 tttatttaat ggagctatac tactacaatc aaacttttca tcatatgcat tttctataca
    11401 atttttaatt tcatcttcag taaaatcaca taaaaattta ctaagtacaa aaaatgctat
    11461 ctgagaatac gttaaattta ctaaatttat aagttcattt tttacattgg gaaattcact
    11521 tggaacataa agaccaccat cacttgatat tccttttatt attgcttgtg atgcagttac
    11581 tttttcttcg gtgcctctcg tacttgaata atacattttt tataatcctc catatatttt
    11641 aaatttttaa ttagctaatt aaattagata cagaatattt taaaaaccga atttaggtaa
    11701 taaaaaaaga ccctcatccc taaaaaaggg acgagaatct gtttcccgcg gttccaccca
    11761 aattgatttt aaaaaatcca cttgatactt taacgcaagt aataaaaacg tctattccta
    11821 ctaaaatcat taagataact tcggatagaa gctcctaggt agttttcaat ccttctaatt
    11881 agggaaacat ttcagctagt agtttccttc tctggtaatc gattcagatt tactcttcct
    11941 aatcaatgcc tttcaatatt cttaatatta ttaattattc tagtttataa aacagacaaa
    12001 gtcaagtatt aatataaaaa tttggaaaaa atatttttta aattgtaaaa aaattgtaat
    12061 caaattgagt attagaaaaa tattttttta acttttttaa aagttt
//
LOCUS       DAEPOG010000120        11971 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEPOG010000120 DAEPOG010000000
VERSION     DAEPOG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.120"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..117
                     /locus_tag="KR432_002635"
     CDS             <1..117
                     /locus_tag="KR432_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0641158.1"
                     /translation="KAKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRYFGK"
     gene            391..651
                     /locus_tag="KR432_002636"
     CDS             391..651
                     /locus_tag="KR432_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641159.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            670..1098
                     /locus_tag="KR432_002637"
     CDS             670..1098
                     /locus_tag="KR432_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0641160.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            1218..1826
                     /locus_tag="KR432_002638"
     CDS             1218..1826
                     /locus_tag="KR432_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH0641161.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            2350..2562
                     /locus_tag="KR432_002639"
     CDS             2350..2562
                     /locus_tag="KR432_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0641162.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            2575..2796
                     /locus_tag="KR432_002640"
     CDS             2575..2796
                     /locus_tag="KR432_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0641163.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2896..5052
                     /gene="feoB"
                     /locus_tag="KR432_002641"
     CDS             2896..5052
                     /gene="feoB"
                     /locus_tag="KR432_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0641164.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            5078..5230
                     /locus_tag="KR432_002642"
     CDS             5078..5230
                     /locus_tag="KR432_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBH0641165.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            5410..6120
                     /locus_tag="KR432_002643"
     CDS             5410..6120
                     /locus_tag="KR432_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0641166.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            6205..6975
                     /locus_tag="KR432_002644"
     CDS             6205..6975
                     /locus_tag="KR432_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641167.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6968..7699
                     /locus_tag="KR432_002645"
     CDS             6968..7699
                     /locus_tag="KR432_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641168.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7733..8692
                     /locus_tag="KR432_002646"
     CDS             7733..8692
                     /locus_tag="KR432_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0641169.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8790..9386)
                     /locus_tag="KR432_002647"
     CDS             complement(8790..9386)
                     /locus_tag="KR432_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBH0641170.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            9632..10231
                     /locus_tag="KR432_002648"
     CDS             9632..10231
                     /locus_tag="KR432_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641171.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(10399..10866)
                     /locus_tag="KR432_002649"
     CDS             complement(10399..10866)
                     /locus_tag="KR432_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641172.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            10977..11888
                     /locus_tag="KR432_002650"
     CDS             10977..11888
                     /locus_tag="KR432_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0641173.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
BASE COUNT         4507 a         1271 c         2106 g         4087 t
ORIGIN      
        1 aaagctaaag aattaggact agatgctata cacgatacag tacatgaaat gtgtaaagat
       61 gaagctagac atggtaaagc attcttagga ttattaaata gatattttgg aaaataaatt
      121 taatataagt taagtagatt tttaggagta tatcatttat gatttttaaa attataatga
      181 tatgctcctt tttaatagtt gtattttata ttttttatag ctataatctt gtaaatattt
      241 aaatcattat aaatatagtt ttaatgaaat taatttttta gttttattat ctctgattta
      301 tgtaataaat agaattaatt tgatttaaac atatatttat gataaaatat gagaaataaa
      361 taagaattta attagaaagg agtatttata gtgtttaaaa ctgtatttga agataaagaa
      421 tatgttttat tatgtaagaa ttattctaaa agtgatggag taaaagcaat atttatttat
      481 ttgatatcta tgttactatt gtttttccaa ggttatttgt atacaacaaa tttaagttca
      541 acaattttaa ctaactcaca aatgatattg tcaatcttga ttttacttat agggctggga
      601 tttgtactac tttcgaagga aaaactaaat acgattggga ttacaatgtg aaatttaaat
      661 aaaagtcttt tgtatggagt tattggtgct actgtactcg taattttcgt tattttaaca
      721 gctggaatta tgcaaggaaa aaaagttgat tttcaattac ctactgtttt tatgattgtt
      781 acatttattg tttctgctat ttcagaagaa attgttttta gagggtatat tcaaacaagg
      841 ttaactggat taataaaaaa tagtgtactt tcatcttgta ttaatgcttt tctttttcta
      901 tctacacatt accctgtaaa atggatttca agtggagatt tttcatttgc aattctatct
      961 ggattttatg tgatatgcct tatattactt aacttttcat gtgatttagt atatcgaaaa
     1021 actaactgtc tgtggggggc atggctactg catatcttgt ataacattgg cacaggagtg
     1081 ctgattttta ctacttagtc aattttactt tgcagaaagt ttatcgtaag aaacggatta
     1141 ttttataagt caaattttag agctagtaaa ttgacatact taaaataatt taaactagat
     1201 gtaaaaaata tcgacaggtg gtggacaatc tgattaagta tatacataaa agtaatatag
     1261 ctattgttgt actgcatgaa atttatggag ttaataattt tataaaagat gtttgtcaaa
     1321 agtattataa aggtgggtat gatgtgtttt gtccagaact cttaggagaa aaaagagttt
     1381 tttcatactt aaaagctcaa gaagcatatg atttttttat aaataatgtt aagtttgata
     1441 tttataaaaa agtagaaaaa cttataaatg gtttaaaatc ggagtataaa catgtatttg
     1501 tagttgggta tagtataggt gcaactattg catggagatg tagtgaaaac tctttgtgtg
     1561 atggtataat ttgttgttat ggctcccgaa ttagagatta tatggatgta attccaacat
     1621 gtcctatttt gctagtgttt gcaaaatacg attcattcga tgttgattat gtttcttctc
     1681 aactttctag aaggcaaaat attcaaattg aaattttaga cgcaaatcat ggttttattg
     1741 atacatactc aaaaaactat tctaatgctc aaacagaagt ttttaatatt aaagaaaaaa
     1801 actttctatc taaatgttta ggctaatatg ttttattata aaatatagaa aatttaatca
     1861 atttgaaaaa cataatgaca ctactaatgt taaaaaatat tagtagtgtc attatatttt
     1921 ttgagataga gatagtcagt agaatcatag aattaattat gtttttatga ttgtattaga
     1981 ttaatattta aattacattg aaaagaaaaa aatcattact atagaataat atctttatca
     2041 aaagaacatt cttatgcaga atatggttat gagtatctaa gagaaatctc aaaatatata
     2101 ggatagcagt atgaattttt atagatttta ttaatacaat aaatcaaatg agaaaaaaaa
     2161 tcaaaagaat acaataatca cttgcaaaaa atatgctatt gtgatagact taaatcaaaa
     2221 ttaggaattg taagaataag gaaaaagata agtaattatt ttaacgagtc aaaatttaag
     2281 aaattgtgat ttatatataa gatagaggtt gtttgtaaat ttaaattggt tgaagaggag
     2341 gaatattata tgatgccatt gatattatca aatacaggtg aagaagtagt tataaagaaa
     2401 atttcgggaa gtgataagac gaggactttt ttaaatagta taggttttgt agtaggtaca
     2461 aatgttaaaa tagtttctaa gatagatggt aacttaataa tagacgtaaa agatactaga
     2521 gttgcattag ataaaaaaat ggcaagtaga ataataatat agggaggtaa tgacatgaac
     2581 agactgaagg atattaagtg tggagagact gtaaaagtca aaaagttaga aggagaagga
     2641 gctacacgaa gacgtataat ggatatgggg attacaagag gtgttaatat atttataaga
     2701 aaagtagctc ctcttggtga tccaattgag gtaacagtca gagattatga actatcaatt
     2761 cgtaagtcag atgcagaaaa aattattgtg gaataataaa gattaattag agtagggtta
     2821 atgtaattat ataatgagaa tgattttctg tatcacaaac agtgatgtat ttaaataata
     2881 atggagggaa tggaaatgtc aataaaaata ggtctcatag gaaatcctaa ctgtggtaag
     2941 acaacaatgt tcaatgggct tacaggctcg tcgcaatatg ttggtaactg gccaggagta
     3001 acagttgaaa aaaaaggtgg aaaattaaaa ggaaataaag atgtggaaat agtagattta
     3061 ccaggtattt attctttatc tccatataca ttagaagaag tagttacacg taattttatg
     3121 ctagatgata agccggatgc agtaataaat attgtagacg catcaaatat agagagaaat
     3181 ttatatctta caactcaagt tttagagcta ggtataccga ccattatagc tcttaatatg
     3241 atggatattg taaataagaa tggtgataag ataaatatta aagaactatc tgaagttata
     3301 ggttgcccag tggtagaagt aacagcagtt aaaggtcaag gaattatgga ggctgctgaa
     3361 aaggcagttg aacttgcaag tagtaacaat aaattaaatt tcaaattacc ttttgttgat
     3421 gaaagtaagg acgcaataga aaaaattgaa aaaataatag aagaaaagac tccatatata
     3481 gatgtagaaa cacgctggtt agctataaag ctatttgaga gagatgaaaa tgttatacaa
     3541 aaacttgata tttctaaaac aatattgaat agtattgaag aaataactag aaattgtgaa
     3601 gatgaattgg atgatgatag tgaaagtata ataacagcca atcgttatga atttattagt
     3661 tcaataattt caagtataat aaagaagaat cgaaaaggta aggaaactgt atcagataag
     3721 atagataaga ttgtaacaaa tcgtatacta gcattaccta tttttgcact tataatgtgg
     3781 ggagtttact atatagcagt aagttcattg ggtactatag caactgattg gacaaatgat
     3841 gtactgtttg gagaaattat tcaaggcaat gtaagtaatt ttctagcttc tttaaatgta
     3901 gcagagtggt tacaaggact tgtagttgat ggtctaattg gtggagttgg agcagtacta
     3961 ggatttgtac cacaaatcat gcttttattc ttattattgt caatattaga agattgtggg
     4021 tatatgtcac gtgtagcttt tataatggat agaattttcc gtaagtttgg tctttcagga
     4081 aaatcattta taccaatgct tataagttca ggttgtggag ttccaggagt tatgtcaact
     4141 cgtactatag agaatgatag agatagaaaa atgactatta tgttaactac attcattcca
     4201 tgtggagcta agattccaat aatagcatta tttgcagggg cattatttgg aggtgcttca
     4261 tgggtagctc catcaatgta tttcttaggt attgcaatga taatcatttg tggaattata
     4321 ttaaagaaaa ctagtttatt tgcaggagaa ccatctccat ttgttatgga attacctcaa
     4381 tatcatgttc caagtgcaaa aggtgtttta atacatatgt gggatagagg aaaagcattt
     4441 ataatcaaag caggaacaat tatatttgtt gcttgtggag taatctggtt cttacaatca
     4501 tttaattggt cattacaaat ggtagatgct ggagatagta tattagctag cttaggaaat
     4561 atagttgcac caatatttgc tcctcttgga tttggaaatt ggcaatcatc agtagcaaca
     4621 gttacaggtc tagtagctaa ggaaaatgta gttggaacat ttggagtttt atttggtata
     4681 tcagatgcaa cagagcaaga cccaacactt cttgcttcag ttgcaagtat gtttacagta
     4741 gctagtgcgt ttgcatttat ggcatttaat atgctttgtg caccttgttt tgcagctatt
     4801 ggagccataa aaagagaaat gggttcatgg aaatggacat ggattacatt aggattccaa
     4861 actttaacag cgtatataat tgctcttctt ataaatcaag ttggaagttt agttttaggt
     4921 actggaggaa gtattgctgg ggcaataata tctatattta tagcagtagc agtagtcttt
     4981 gtagttttaa cttattcaaa taaaaatatg aaaaaagaaa aaatgggtaa attaagttat
     5041 atgaaaaatt aattcatata aataaggagt gatgaatatg gctacattta ttatagcagc
     5101 tattgtagta gtattaatgg cactagctgt aatgtatatg gttaaaaatt caaaaaagaa
     5161 tggtagcagt tgtggatgtg gttgttctgg atgctcaagt agtaagagtt gtcatagcgc
     5221 taagagataa ataggataag agcctgataa aaagttaaat tgctttatta tcaggttctt
     5281 attttttata taaataacaa atttcatatt tgaagtaggt tagactatgc tgtaatttta
     5341 ctgtataatt atatataaat atatgattag gagggattat tgctaaatta agcaatataa
     5401 gtgaatatta tgtttaaatt tgatgttaca aacgagctta tgtgtatagc aaggggaagt
     5461 ggtaaatttt ttgcaaaaaa aggtgctatg gtagccttta aaggaaattt taattttgaa
     5521 aaattgctgt taggtccaag taatggcggt ggattgggaa gagcattatt aggtcatgtt
     5581 cagaggtcat tgacaggaga acaaatgcca ataatggtgg ttgaaggtga aggtgaaatt
     5641 tacctagctc aaaatgctta ccatgtagat gtaatatcaa ttgacccagg tgattccatt
     5701 tatgttgaga gtgaaaactt attggcattt tctgaacaat taaattacag tgtaaaattg
     5761 attggttcag gggtaatatc tcaaaaaggt ctttttacta cacatttagt aaataaaaca
     5821 ggtcaaaatc aagatgttgc tataataaca gatggtaatc ctctaattct tgaaggacct
     5881 tgttgtgttg acccagatgc attagtagct tggactggaa gagaaccata tcctaaagta
     5941 tcaaaactat catggaagac ttttattggt cagacttcag gagaatctta tcatatggaa
     6001 tttgttgaac ctggacaaat agtcattata caaccatctg aaagattgtc tggcttaaaa
     6061 acagattcta caccttctga tagaatgagt tcaggtggat taagacttgg catagattaa
     6121 atcttatgat atagcttaat tattatttaa gatattaaaa aataaaaagg gtagcaggga
     6181 tagatatgtt aaaaggagtt ttatatgttt gagaaaattc ataaaaggtt taaaggatat
     6241 tatatttttt taataatttt aattttatgg aagatagtat gtgttttggg tatatggagt
     6301 agctatattc tgcctcctcc tgaaatagtc ttagatactt ttataaaaat gatatatgac
     6361 ggttctattt tcttaaatgt ttatgccagt gtaagaagga tattaatagg ttttttaata
     6421 agttcattga tggctatacc acttggagtt gtatttggag taaataaaaa aatgtatgaa
     6481 tatttcaaac cactatttga atttatgcga agtacacctc cactagcttt ggtgccaatg
     6541 cttattttat ggtttggtat aggtgaagaa tctaaaatta taattattat tttggcatcc
     6601 ttttttccaa tatttttaaa tacattgaaa ggtataaaaa attgtgacaa taaattaata
     6661 gaagtaggta aatcttttaa tttatctaaa aagagaattt tttataaaat aataatccct
     6721 aattctatgc tggatattgt tgttggactt agtttagggc ttgggtatag ttttagggct
     6781 ataattggag cagagcttat tgcagcttct tcaggactag gatatttgat ttctgatgga
     6841 aaagatatgt ctagaactga tgttgtattg gtaggaattt ttgttattgg atttttaggt
     6901 atactttcag attatctatt ttcaaagata ataacaaaat ttagcaaagg gaagcaggtt
     6961 gatttaaatg gttagaagtg gatttttgct agagaatata tataagaaat atttagttga
     7021 taataaagag catttagttc ttgataatat atctttaaat atatctagtg aagagataac
     7081 tgttatatta ggtgaaagtg gatgtgggaa aacaacgcta cttagaattt tagcagggct
     7141 tgaaaatgct actagtggaa acatatattt ttttaataat gataaaaagt gtactccaaa
     7201 agtaggaatg gtatttcaag aaagtagact tatgccatgg ctgaatgtaa gtgaaaatat
     7261 acttttacat actgaaaaag ataatagaaa taaagttgat ttagacaagt atttaaaaat
     7321 gatgaagctt gaaaagttta aaaactcata tccaaatgaa ttatcaggag gaatggctca
     7381 cagagtaagt atagcaagag cactttcctt caatcccgat attttgctga tggacgaacc
     7441 atttgcagcg ctagattact tcacaagaag aaaaatgcaa aaagaagttg taaatatcca
     7501 taaaaatact aaaaaaggtg tagtatttgt aactcacaat attgaagaag ctatggaaat
     7561 agcaaagaaa ataattgttt ttagtaaaaa taaaaggatt aaacaattta gtgtcgaaga
     7621 tgaatataat agagatttaa ctaaaaacta ttatataaac ttgaaaaaag aaatattaag
     7681 ggaattgggg gagttttaat tgattaaaag aaacaaaaag attttagcag taatgacatc
     7741 tttaataatg gtgttggttg ggactgtagg atgttcaaca acaaatgata agaaagatga
     7801 acctgcaaaa aaagaggcta gtttacctaa ggaaataaat ttaacttatg taaaatcacc
     7861 acttaatgtt cctagtatat tagaaaaaaa ggatgattta tttggaaagg aatttaaaaa
     7921 ggacaatata aaagtcaact tccatgaatt gacaacagga ccagagcaaa ctcaagcatt
     7981 agcagcaggt gagctagact ttttacatgc attaggagga acgtctgcaa taatagctgc
     8041 atcaaatggt gttggtctta aaattacgaa tatttatagc cgttcaccaa aaggatatat
     8101 gcttataact aataatgata atattaaatc tataaaagac ttaaaaggta aaaaaattgt
     8161 aggtccaaaa gggactatac ttcatcaact gttgattgct gcacttgcta aagaaggcta
     8221 tactatagat gatgttgaat atgtaaatat ggatattcct agtgctgctt ctgcattaga
     8281 aagtaaaagt gctgatgtag caatgcttgc aggtccagtg gcattgaaaa cgataaattc
     8341 aggtgccaaa atgattgtaa atggagaagg attagtaagt ggaataatag ttactgctgt
     8401 aagtgatgac tttgctgaaa aatatcctga attagtaaaa agatttatga aggttcatga
     8461 agaaacttta aaatatatga atgaaaacaa ggatgaagtg atggatgttg tatctaaaga
     8521 agttggttta tctttagatg aaacaaaaga aatgtattct tggtatgact ttagttctaa
     8581 aataacggac aaggatataa aagaattaga agatacacag gaatttttga tgtctaatgg
     8641 aatgcaacaa aagaaaataa atattaaaga tatgttgtac aatcaaaatt agaatacttt
     8701 aaaatattaa agtggaaaat acacccttat ttattatgga aactgtatgg taaaagaggg
     8761 tgtattttta ttattataca aaaatacaat taaaaacaac ttactatttt atctacactt
     8821 atgtagtcta gtaattttct cattttctta cggtgaagta aaaactttga atatgtgtcc
     8881 tcggattgtt ttgtgtcacc ataaactttc caatatttaa tcatagaaac ttcataatta
     8941 ctatatccca taaatccaat cacatcttta agaggatatc ctaaaaaaat acctatttca
     9001 tcaggaaaaa catcactctt aagtttattt actaaatgct ctaaataagc attaacattc
     9061 gtattttgtt tgtatcctaa aaactttaaa aagtttactg tttttttaca ttttaatttt
     9121 tctgataatg cttttttatt tacaaataag atttttaatc cctttttttc tttatcaata
     9181 actataaaat ctattttctt acatttatat agatatttat aaatatcatt aattttttcc
     9241 tctctattta catcagtaaa cgaaatattt attatttcag caggtttaga gcctaaaaag
     9301 actggaccta aaacctctaa aagccatttt atatatgatg attcaatttt atttttacaa
     9361 caaaattgtg aattacaaca agacataaaa gataacctcc ttcaaatgaa aattaatatc
     9421 aagtaattag attatactat gaatgataat caatatcaat aataaaatta taattttttt
     9481 atttaattat ttattcatta tgatttccaa aaaaataaat tataacttgt ttaattttag
     9541 taaataaaaa ataaaaatat agaaatacta aattaagaaa gaaaattatt aaaatttttt
     9601 gtagtacaaa acatctagga gggtaataaa aatgaaatta aaaagaactt tatttgtagg
     9661 attagcatta gtattagcta taggaataac tgcatgtgct agggataatg aaaaaccaaa
     9721 tgaaaatgca ggaaaaaatc ctaatgaatc tgtaaataat aatgaaggat atatgaatta
     9781 ttattcaaca agttataatg attatatagc aggtctaaat agatattcta tttatgatac
     9841 tccagaaagt ataaacaatc agtttaaaga taaagaatat ccaggaaatg aaaaatatct
     9901 aagtgatgta aaggctgcat ataaagatag tagagataaa atacaatcat ttgtaacatc
     9961 tcttaaaaaa gacggtaaaa cagaagatac agagttaaag agaatgaatg atgacctaat
    10021 agctgaaggg gaaagattag taaaagatat agatgagaga atagaaaaat tagataaggt
    10081 atctgatgaa gatatgaaaa aaggtcaaaa tgattttatg aaactagttc atggaactga
    10141 agatgttgga aacgatattg gtagtggttt tagaaaaatg ataaaagata tgaatgatag
    10201 attgggaata acaggaaata ataaaaaata atagagtaaa tagttttaaa gttagaaata
    10261 aatatttgaa atatagcgaa gccttgtaat taatatattt tttggcttcg cttttttgta
    10321 tgttatcaag atactaattt ttatcttgta acctcatatt ttcattatag tttatttaga
    10381 ggttattttt ttatgttttt acttgttttt tctcttttta aatttttgtt ttgcaaaatt
    10441 gtaagattct tctattaaag ttttaaagtt ctcaaatgtc ttttcagtag ggcttaggac
    10501 gcatatccat cccatccatg agtagatagg atgtggcatg attgtgtcca attttgtaaa
    10561 atcaaaatcc atatcaacta tttgccctac acctggacgc tttggaatat atccaaacat
    10621 ttcaataaaa gtttcttttt ttaatcctat attcacacgg tatacatttg gacgactaac
    10681 caacgaacct ttatcatgat tactgtcttt ttctttaatg gtaagaacat atacccctcg
    10741 ctttaaaaca ccattaggat tgtagtaaat tgctcgttcc ccccaactat ccattagtac
    10801 aacatcatct aagtttgata gacaataatt gagaatatca tcagccttaa tagtatcatt
    10861 ttccataaaa tatcaatccc ttctttcttt aatattatta ttgctttaga tgcaagaata
    10921 tcttatcata taatggtgac aattattgtc actatattta aattaggagt atgaatatgt
    10981 caagagctga gcgtttaatt gaactgatga taacaataaa tgcaaaaaga agttttacag
    11041 cgggagagct agcagaagaa ttttctgtat caaaacgtac tatactaaga gatttacagg
    11101 tattggaaag tgttggattt ccactatatt caaaagttgg agcggctgga ggatatcatg
    11161 tacttaaaga acgtatactt ccaccaatta cattttcaga gagtgaggta aaatctatat
    11221 tctttgcata tcagtcacta gagtattata atgatttacc atttgagcaa gaaactattt
    11281 ctgttttaaa gaaattttta aattgtatac caaatgatat tcaatataat attgagaata
    11341 tgagacgtaa acttgttttt tggacaccag ataggcattg ctcaacacca ttattgaagg
    11401 aattgtttaa tatagtaatg aatgaattta caattaaaat tgaatattca tcaaagcaaa
    11461 aaaatagtat acgtaccatt gttcctattg gtttgtatgc tatgaatgga ctttggtatt
    11521 gtccagctta ctgtataaaa tcagaatctg ttaaggaatt tcgtgcagac agaattgtga
    11581 agattttgag tatagaaaac ttatcaaata agaaatataa ggttttatct tctatacatg
    11641 attatcttaa gaatatggaa gtaggtacag attatcatat taaaattaat ctgacagatg
    11701 agggagtaaa acgttgtgaa acagaatttt tgttggcaag aggattgaaa attttgtcta
    11761 agggtggata catagatatg tatataccaa aatctacact taattgggtg gctgaatatt
    11821 ttttaacttt tggaaaaaat gctacaataa tagaacctat agaacttaaa catttgatta
    11881 aaaaatagct acgcacactt gtgactttag tcatgagtta gtagctaaat tagtcagcat
    11941 atagggaaac ttatatgtag agataggata a
//
LOCUS       DAEPOG010000121        11846 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEPOG010000121 DAEPOG010000000
VERSION     DAEPOG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.121"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(384..1787)
                     /locus_tag="KR432_002651"
     CDS             complement(384..1787)
                     /locus_tag="KR432_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0641174.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="KR432_002652"
     CDS             2664..3173
                     /locus_tag="KR432_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH0641175.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="KR432_002653"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="KR432_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH0641176.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="KR432_002654"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="KR432_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH0641177.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="KR432_002655"
     CDS             4117..4662
                     /locus_tag="KR432_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641178.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="KR432_002656"
     CDS             4649..4822
                     /locus_tag="KR432_002656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641179.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="KR432_002657"
     CDS             5098..7023
                     /locus_tag="KR432_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0641180.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="KR432_002658"
     CDS             7424..9328
                     /locus_tag="KR432_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0641181.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            9716..10495
                     /locus_tag="KR432_002659"
     CDS             9716..10495
                     /locus_tag="KR432_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0641182.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(10805..11626)
                     /locus_tag="KR432_002660"
     CDS             complement(10805..11626)
                     /locus_tag="KR432_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBH0641183.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
BASE COUNT         4675 a         1345 c         1978 g         3848 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgattcta caatataatt aacactactt ttttaaatat
     9421 aataaatact gattctacaa tattattaat actacttttt ataatataat caatactact
     9481 ttttacaata taatcaatac tgattctata atataattaa cactactttt taaatataat
     9541 caatactgat tctataatat taacactatt ttttatgcta tataacaatt attgaaaatg
     9601 atataaatat aatatctcaa atgatatgaa tataacatct gaatagaggg tgttttattt
     9661 gtaaaaaata agtttacagt ttgagcgtat aaacttatgg taaaggagaa taattatgaa
     9721 ttataagaag attgcaatta taacgggtgc taatagtggt tttggtaaag agtttctaaa
     9781 gttattaata aatgaagaag aaataacaga aatatgggca gttgcaagaa acaaggcgag
     9841 attaaatcaa ttagtagatg aatttggaag taaggtaaaa gttttttcaa aagatttatc
     9901 aaaaatagaa gaagtaaaag aaattggtac atttttaagt aaagaaaatg tatgtataaa
     9961 ataccttata aacaatgctg gttttgcaaa attctgttca tacaatgatt taagtataga
    10021 tgaatctatt aatatgattg atttaaatat tagtgctgtt gtagtattgg gacttatatg
    10081 tattccatat atgcaaaagg gaagccatat tatgaatatt tcttcacaag catcattcca
    10141 gccattgcca tatcaaaata tttatagttc aactaaagcg tttataagaa attatacaag
    10201 agcattaaat gttgaactta agagtaaagg gataaatgca attgctgtat gtccaggttg
    10261 gatgagtaca aatctattta agagaggtat agttgtagct gaaaaaggga caaaaaattt
    10321 cccaggcata gtgtctccag acgttgttgc caaaaaagca ttgaaggatg caaagaaaaa
    10381 taaggatatt tctgtgtatg gtataaatac aaaatccagt catttattag caaaactatt
    10441 gccacaaaaa atgatgatga aggtttggtt aatgcaacaa catattaagg aataagagtt
    10501 tcggtaatta agtatcagtt ggagattcaa tctattatat attataagaa gttttgttga
    10561 gcaaataata attgtgacaa aacggcttat ctagtgccga taagccgtct ataatgtata
    10621 aaaaatatat ttttgatttt actttattta gatattgaag tattaaaata tgtaggactt
    10681 gaaattttat aaagataaat actatgatat tttgtaaaaa ctgagatatg ttgatattac
    10741 atagtttaac tatactgtta aattgaagtt ttcttatttt gcattatgct tattacttaa
    10801 tgatttagac tataatttcc ataccatcat atgcaaaata aatgttatca agtttctttt
    10861 ctaattcact gtaataacca tatgattttc cccacatttc gtcaatatgt gtaatgataa
    10921 tctttttaat tctataatag tttttaattt caacaatctc atctaaagta aatatttcat
    10981 ctttaaatgg tgtgtcttca agttttgtac catcatttaa tataccatca tcagaaacta
    11041 gacttattat taaaacatca gcatcaaagt acatatcact atgagtaaat ggcttgggat
    11101 tacagcaggc atatatgact tttttttcat tctctttgat aacatagaag gttatattcc
    11161 tgtctgggtt attgttaatc aaatcaatat caaaattatt tatctttaca tgcgaagttt
    11221 cattaataga aatgcaattt ataacatcac cataatattt aagacaatcg agattcgttc
    11281 ggtttatatc ttcaattact tcaggaagag cataaaagcc aattggatta ttttctcctg
    11341 tgataaagtc acatcctatt ttttcgacaa ttctaatacc cttaacatgg tctgggtcat
    11401 ggtgagaaat tgaaatatgt tttacttccg atattttttg tctgttacaa gctactgtaa
    11461 tatcttcagg tgtgtctatc agcattttta tatcatcaat atataaactt ggtcctaatc
    11521 gttcatatct tccgcctttt tgcctggctt cttcacatat tttgcattta caaaatgaac
    11581 ttggaataat tgacataccg ccactaccaa caatttttaa tttcaaagtt attacaccac
    11641 cttctaaaaa tattagatta tataaataat ctacaatcac aactgattaa caaaattatg
    11701 agttaataaa ttatatcaca tgtaaatatg aaagaatagt atgaaatttt tataagatta
    11761 tattaaatat ttctcaatga aattgacttt gtatatgagc cgaatgactt tattcaatat
    11821 agataagaca tttttcttgt aataag
//
LOCUS       DAEPOG010000122        11498 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEPOG010000122 DAEPOG010000000
VERSION     DAEPOG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.122"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(72..497)
                     /locus_tag="KR432_002661"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="KR432_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="KR432_002662"
     CDS             complement(970..1257)
                     /locus_tag="KR432_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641184.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="KR432_002663"
     CDS             complement(1277..1552)
                     /locus_tag="KR432_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641185.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="KR432_002664"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="KR432_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="KR432_002665"
     CDS             complement(2818..5385)
                     /locus_tag="KR432_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0641186.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="KR432_002666"
     CDS             complement(5388..5993)
                     /locus_tag="KR432_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641187.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="KR432_002667"
     CDS             complement(6367..7827)
                     /locus_tag="KR432_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0641188.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="KR432_002668"
     CDS             8158..8736
                     /locus_tag="KR432_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641189.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="KR432_002669"
     CDS             9028..10278
                     /locus_tag="KR432_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0641190.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="KR432_002670"
     CDS             10439..11008
                     /locus_tag="KR432_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0641191.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3802 a         1994 c         1588 g         4114 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatata ttaaaataaa tatatatatt
    11281 aaattcttat acaccaaatt tttacattaa acttctatgc accacattta tacatattaa
    11341 attcttatat attaaatttc tatgcatcac ttttatacat attaaattcc tatatattaa
    11401 acttttatgc gtcactttta tacatattaa attcctatat attaaacttt tatgcatcac
    11461 ttttatacat attaaattct tatatattaa acttttat
//
LOCUS       DAEPOG010000123        11369 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEPOG010000123 DAEPOG010000000
VERSION     DAEPOG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.123"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            585..2663
                     /locus_tag="KR432_002671"
     CDS             585..2663
                     /locus_tag="KR432_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBH0641192.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2663..3442
                     /locus_tag="KR432_002672"
     CDS             2663..3442
                     /locus_tag="KR432_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0641193.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3435..4244
                     /locus_tag="KR432_002673"
     CDS             3435..4244
                     /locus_tag="KR432_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0641194.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4264..4746
                     /locus_tag="KR432_002674"
     CDS             4264..4746
                     /locus_tag="KR432_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641195.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4767..5171
                     /locus_tag="KR432_002675"
     CDS             4767..5171
                     /locus_tag="KR432_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0641196.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5327..6487)
                     /locus_tag="KR432_002676"
     CDS             complement(5327..6487)
                     /locus_tag="KR432_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0641197.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6695..8245
                     /gene="xylB"
                     /locus_tag="KR432_002677"
     CDS             6695..8245
                     /gene="xylB"
                     /locus_tag="KR432_002677"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH0641198.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            8341..9678
                     /gene="xylA"
                     /locus_tag="KR432_002678"
     CDS             8341..9678
                     /gene="xylA"
                     /locus_tag="KR432_002678"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH0641199.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            10200..>11369
                     /locus_tag="KR432_002679"
     CDS             10200..>11369
                     /locus_tag="KR432_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBH0641200.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRK"
BASE COUNT         4381 a         1256 c         2050 g         3682 t
ORIGIN      
        1 agacactctt tttttatatt aaatcaagta tattaaatca aatatatttt aatcatttta
       61 gcaatgaaaa ataaatttat ctatattttg agacaacatc ttttttagtt ttatttattt
      121 gttgtcgttt ttgacttggt atacaattat atattttctt aaataatttc ataaattatt
      181 tataattata aaatccattg ttttgaacaa tatctaatat attattatct gtatttataa
      241 ggtcactata catattttca agtccaatat tatctacata ataggcaaat tttattcctg
      301 tatgtgttta aaaaaaacta gatgtatata ggtatcatca cagatattaa aagtataaat
      361 aaaagatatt aagaacgact ttcaagcaac atattagaaa attttactaa aatccggaaa
      421 gcgattgaca aacaaagtca aataaagtat attattggta agttagttta atgtacaaat
      481 gaattatata taaaacctaa tttataaata acgatactaa acgacattat ttaacttaag
      541 ctacacaaag atagaatatt ataccattga tttaggaggt agccgtgata aaaatatctg
      601 aagactatat tgaaaaaaga tttgacaatg aactattgcg aatagaagca tggggaaaaa
      661 actctttacg tattaggtcg tttgtagacc aaaattttgt tgatgaaaac tatgctctta
      721 acgaaaaacc taagttaaat aaagatgata ttacaattaa taaaaatgaa gatggaagtg
      781 caatcattaa aaatggaaag ataaaagctg tcttagacca tagagataga attacttttt
      841 ataatgataa aaatgaaata ttattgaaag aatatattcg attaagagct gtaaaacatg
      901 atgatggtgg agaagatgta gggacaattg agattaccaa agactttaac tctactctaa
      961 agttaaaatc aagagaatat cgaccaagct atagtggaga atttgaagta actactagat
     1021 ttgaatctga gccttctgaa aagatttttg gaatgggaca atatcaacat aaatttttag
     1081 atttaaagaa tactgttttg gagttagccc aaagaaattc tcaaatatca gttccttttt
     1141 atatttctag tctaggttat ggacttttat ggaataatcc gggaattgga aaagtatctt
     1201 ttgctaaaaa tatgacagaa tggaagatgt tttctacaaa ttttatagat tattggatta
     1261 cttgtggaga aagcccaaaa gagttaaata aaaattattc tcaagttact ggaactgttc
     1321 ctatgatgcc tgaaaacctt ttaggactat ggcaaagtaa actaagatat agaacttcag
     1381 aagaagtttt agatgttgtg aaggaatatt ctaaaagagg aattaagctt tcatcaattg
     1441 caattgacta ttttcattgg cctaagcaag gtgaatataa atttgactta gattattgga
     1501 aaaatcctaa agaattagtt aaaaaattga aagaagagta ttcagtagaa ccaatagtat
     1561 ctgtatggcc tacagttcaa agtgatgcag aaaactacaa tgattattta gaaaatggct
     1621 atttagtcaa tgttaatcgt ggtgttagaa tgacaatgca aattcaagga aatacagtct
     1681 ttgtagatat gacaaatgaa aatgcaagag aatatgtttg ggatagaata gataaaaatt
     1741 ataaacaact tgggattgac tattattggt tagatgtagc tgaacctggc tattctgtgt
     1801 atgattttga taactataga tataaaaaag gaaatgtttt aagttgtggt aatatttatc
     1861 ctattgatta tttaaaaatg atttatgatg gtttacataa tgacatagag tctgttgtaa
     1921 ctcttgtaag aggagcttgg gcaggagctc aaaagtatgg agctttagta tggtctggtg
     1981 acattgattc tagttttgag gcttttaata atcaagtaaa tacagggtta aatatgggac
     2041 tggcaggtat accatggtgg actactgata ttggaggttt ccatggagga aatcctaaag
     2101 acccagaatt tagagaactt atggtaagat ggttccaata tgctactttt tcaccaattt
     2161 tgagaatgca tggagataga ttgcctcata gtaaaccatt atcaaataaa ggtggaggct
     2221 caatggtaac tggagctcct aatgaaatat ggtcttatgg tgaagaagtt gaagtaattc
     2281 ttacaaaatt cataaaaatt agagagagct taaaaactta tttaaaaaaa ctgatgaaag
     2341 aagcgcatga agatggaaca ccagttatga gaactttatt ttatgaattt cctgaagatg
     2401 ataaaacttg ggaggtggat aatacatata tgcttggaga tgaaattttg gtagctccaa
     2461 taatgaatta taaagataga agtagaaaag tatatttacc aaaaggacat acatgggaaa
     2521 atatatttag tggagttagt tatgaaggtg ggaaaactta tgaagttgaa tgcccgctgg
     2581 aagaaattcc tattttccta aagcaagatt ccagttataa ttttaaagaa ctaaaaaata
     2641 ttttgggaga ggtgaaatca taatggagcc acaattatgg caattattat taatcgtact
     2701 atatggattt tttataaact atgaaaaaaa ttcaacaatg tttggaacat atcaaccagt
     2761 aacagcaggt tttattactg gtcttatctt aggagatata aatacagggt tatatattgg
     2821 aggtacatta cagctattat cattaggtat tagtaacttt ggtggagcat caatacctga
     2881 ttatcaaaca gcaagtatag tagccacatt tataactatt acgactaagc aagaagcatc
     2941 tgtaggaatc tctataggta ttccagttgc tttattgatg gtacaattag atgttcttag
     3001 aaatactatt ggtatatggc tagttcataa agcagaagat ggagctaaaa aaggaaatta
     3061 taaaaatata acctatatgc aaatgttagg agtactatta acagcagcta caacaggtat
     3121 tccagttgct ctatctgtaa tatttggacc ttctttaatt aataccattt tgaaatatac
     3181 acctgaatgg ttaacaggtg gtttaacagt agctggagga ctacttccag cagtagggat
     3241 aggattatta cttagatatc ttcctgctaa ggagtatttt agttacttag taattggttt
     3301 tgttttagca gtatatatga aagtaccatt attaggtgta gctttaattg gtggagcaat
     3361 tgcacttata atttacaaaa agaatttgga aaatcaagaa caacaatata ctgtagtagg
     3421 aggtatggac gaagatgagt gataaggtat cattaaataa atcaaatgaa aaggtgaaaa
     3481 aaagtgtctt atggcgctgg ttttttacta gttctgtttc ttcaaattat gagaagatgc
     3541 aggccttggc atactgttat gcagtactac cttttttaaa agtagtatac aaagataatc
     3601 cagaaaagtt acaaagtgct gtattaaatc atttacaatt ttttaacact aatccttggg
     3661 ttgcccctta tattttgggt attaatgtag caatggagga gttatcaagt gaagagactg
     3721 aagaggctgt tgcttcaatt aaaacaggct tgatgggacc agtagcaggt ttaggagata
     3781 gtttatttgt cgttattccg tggacaattt ttggagctat agcagctaat atggcgatag
     3841 atggaagtcc atttggaatc ttactgtgga ttgtagtgag tgtagcattg aaattattaa
     3901 gtataccact atttaatgct ggctatacat caggtactaa gctaatatct tcaattgaaa
     3961 aaagtttaaa gattttaact gagagtacat ctattttagg tttaatggtt gtaggtgcat
     4021 taataccttc agttgtaaaa gctaatgttg ctttgaactt tcaacaaggt gattttacaa
     4081 tgaaaggtca agaaatatta gaccaaatta tgcctgggtt agttcctgca attttagttg
     4141 tgattgttta ttgggctctt aaaaagaata taaaacctat atacttaatt ttaggagtaa
     4201 tggttgtttc aataatttta tatgcattag gtattttaaa ataaggaaag gatgatatta
     4261 gatatggaaa tgaaaggtat taaaaatatt cgtatagatg accgtttaat ccatggtcaa
     4321 gttgcaacta tgtggagtaa taaattgggt gttacaagat taatggtagt aaatgatgca
     4381 gtagctaaca attctgttca aaaacaagtt ttaagaatgg ctacacctgc aggtatagcg
     4441 tcatctataa ttacagaaga gactgctata aaaaatatta ctgcaggaaa gtatgaagga
     4501 caaaatgtat tattaattgt taagtctcct gtggacctta ttccatttgt agaggcagga
     4561 tttaaaatac ataatataaa tgtaggaaat atgtcaagta gaaaagatac aacagttcta
     4621 agacctaata tcagtgttac tgaagaagag aaagaagcat ttaaaagact tttagatgaa
     4681 ggaattgaaa ttacaactat aatgacgcct gatgataaga aaacttatct tgtagacatc
     4741 ttatagattt aaagaggtgt attaaagtga atagtattat tttgatgagt catggcaata
     4801 tggctgaaga agtcttaaat tctgcaaaaa tgataatagg ggattctata gattatccta
     4861 ctgtaaatat gaaacaagat gatggaatag aaggaacaat agaaaaatta aaaaaagctt
     4921 tagaagtgta caaggaaagt tcagaaataa ttataatggc agatttacta ggtggaactc
     4981 catgcaatgc tgctttatta gtagccaatg atgacagtcg aattaaaatt atttcagggc
     5041 taaatttagg tatggttatt gaatcagctt ttttagatac aaaggaattg gcagcaaaat
     5101 tagagaaaat aggtaaagat aatatttcta tggttgaaca aaatgcaagt ttattatctg
     5161 atgatgagta ggaagattat attaatattt aaaacaaaat acttgtgtta atacttaatt
     5221 gatatacaat ttttaaaaaa tatagctaat cttgcataaa ctatataata aatctaattt
     5281 tagaatgtat aatttaaagg gttatggctg tatatagtca taacccttat atttcattaa
     5341 ttaaagttaa attttgtata ttcaaaaagc tcttgatatt tacggcagag ccaccatata
     5401 gagatgcctc agaccctaat gatgaaattt ctatatttat tcctttgtta aaagagctaa
     5461 ctaacatatc ttttgtaatc tgtaatatct cagggatatc actaattatt tggctattta
     5521 aatatattat ttcaggagca taagttgtaa tagcattatt tatagctatt gttaagtaac
     5581 tacaaaactc atgaataact tttttggcat tctggttatc ttcataatat agttgcttta
     5641 ctatatcaga atctatttta ggaatatttt ctaaagaaga aagctgttca aatacctttt
     5701 tttctgaaca atactgttct aaacagccac gatttccaca aggacatagt ttaccattag
     5761 gcatgatgat agtgtgacca atttctccac tcataccatt tctgccgcta tataatttat
     5821 tatttattat aatgcctgaa ccaaatccac tgtgaatact gagactaagt agtgagttat
     5881 gtacagttga aaaagtattc tcagctaaag ctgttaagtt tgcttcattt tctatatgaa
     5941 taggaaagtc atactttttg ctaaggatgc tatataagtc aatctcatta agattataat
     6001 atggagtaaa caatacttta ttttcacaag taatcccatg tatagccaaa gtaagaccaa
     6061 ttaccttata aggagtgtct attttggata tgttataact atttataatt tcatctatca
     6121 attgtataac attgtcttta cttacttgta tatctgttaa tttcttagaa tttataatag
     6181 ttccatctaa gtaagataga gaagaaaata tatagtcgta tccaatgtcc atacttaaag
     6241 aaatccctgc acatttatta aatactaata atatgggttt tcttccgcca ctatgagtac
     6301 tatttccaat tcctatttca tgaactagag attcatcaat aagttttttg gtaatagcgg
     6361 atatagttgc tttattcaat cctatagtag aagctatact tgccctagaa ataggaccat
     6421 tttttataat ttgttcaagt accaatcttt cattcatttc tcgaatagtg tatttatcag
     6481 taaccaattt gatattcctc cttaaaataa tattgtaata ctttttacac aaaaataaaa
     6541 ggttattttg cattgacaaa gataattaaa tattttatta ttagttcata agttagttta
     6601 atatactaac aaaaataaag caagtaaaat atacctaaaa tataaaaaaa ttaggatagg
     6661 aaaacgatag ttatgaagtg gcattcaagg agggatgaag ttggagtatg tattaggtgt
     6721 ggatattggt acatcaggta ctaaaacagt tttatttgat aaactaggaa atattataaa
     6781 aagttgtaca tatgaatatc ctcttataca agagaaatca ggttgggcag agcaggatgc
     6841 aaatgattgg tggaaagctg tagtcgaatc tataagagaa gtagttcaaa gttcaaatat
     6901 aagcagtgaa tgtataaaag gaattggatt atcagggcag atgcatggtc tagttatgct
     6961 ggacaatgaa ggtaaaacat tgagaaactc tataatatgg tgtgaccaac gaacagtaaa
     7021 agagtgtgag gagataactg atttagtagg tgaagaaaga ctgattgaaa taactgctaa
     7081 tccagcgctt acaggattta cagcatcaaa gatattatgg gttagaaaca atgaaccaga
     7141 tatatatata aatactaata aaatacttct tccaaaagat tatataagat acaaattgac
     7201 aggtgaatat gctacagagg tatcagatgc aagtggtatg cagttgctcg atataagaaa
     7261 aagagattgg agcgatgaag tactagaaaa gttaaatata gataaaaatc ttttaggtaa
     7321 agtttatgaa tcacaagaga ttacaggtta tgtgactaga gatgtagcaa gtttgacagg
     7381 gcttaaagaa ggaacaatag tggtaggagg agctggagac caagcagcag gagctatagg
     7441 aaatggaata gttaaagatg gagtagtttc atctactata ggtacatcag gtgtagtatt
     7501 tgcttatacg aaagagccaa aaattgataa agaaggtaga gttcatactt tctgtcatgc
     7561 aataccaaat acatggcatg ttatgggagt tactcaagga gctggattgt cattaaaatg
     7621 gtttaaagat aatttctgtc aaagtgaagt ggaagtttca aattctttag gtgaagatgt
     7681 atatgagatt ataaataatc aagtaagtca agtaccaaca ggatgtaatg gattgcttta
     7741 cttaccatat atgatgggag aaagaactcc tcatcttgac ccttatgcaa gaggtgtgtt
     7801 ctttggatta tctccaatcc attctaaaaa agaaatcgca aggtcaataa tggaaggtgt
     7861 ttcatatagt cttaaagact gtatggatat aatagagaat cttaatatag acgttaatga
     7921 agtaagagcc tcaggtggag gtggaaaaag taaagtatgg agacagatgc aagcagatat
     7981 gtttaatcaa gatgtatata ctataaattc aagtgaagga ccagcacttg gagttgcaat
     8041 tcttgcatta gtaggagctg gaatatatga aaatatacaa aaagcttgtg atgctataat
     8101 aaaaacttca actaaattag aaccaataag tgaaaatgta gatacataca agatatatca
     8161 tagattgtat aaaagaatat acaaatcttt gaaagatgac tttaagttat tagatgaagt
     8221 agttaataca aaaataaata attaaattag cttaaaaaga aaaataaaga tagaaaatag
     8281 ttttaaatta agaaaacata cgacatatat tatatgtgta aaatattagg aggacatgaa
     8341 atgagtgaaa tatttaaagg aataggacag ataaaatttg aaggagtaaa atctgacaat
     8401 gaattagcat ttagatacta caatcctgaa caagtggtag gtaataagac tatgaaagaa
     8461 cacttaagat ttgcaatgag ctattggcat actttatgtg gagagggtaa tgacccattt
     8521 ggagtaggca cagtagagag accttggaat aatgtaacag acccaattga gatagcaaag
     8581 attaaagttg atgcaggatt tgaatttatg tctaagatgg gaattgagta tttctgtttt
     8641 catgacagag atatagctcc agaaggaaga gatttagaag agacaaataa gatattagat
     8701 gaaatagttg agtatataaa agttaatatg gaaaagacag gtataaagtt attatgggga
     8761 actgccaata tgtttggaaa tccaagattt gttcatggtg catctacaac atgtaatgca
     8821 gatgtatatg catatgcagc agctcaggtt aaaaaagcta tggaaataac taagtactta
     8881 ggaggagaaa attttgtatt ctggggtgga agagaaggtt atgaaacttt acttaacact
     8941 aatacagaac ttgaaatgga taactttgca agattcttac aaatggctgt agattatgct
     9001 aaggaaatag gattcactgg ccaattctta atagagccta agccaaaaga gcctactaaa
     9061 catcaatacg attttgatac tgctactgtt ttaggatttt taagaaagta taatctggat
     9121 aaatacttca aaatgaatat agaagcaaac catgcaacac ttgcaggaca tactttccaa
     9181 catgaattaa atatagcaag aataaacaat gttttaggta gtatagatgc aaatcaagga
     9241 gacctattat taggttggga tacagaccaa tttccaacaa atatatatga tgcaacactt
     9301 gctatgtatg aagtgttaaa gcaaggtgga atagcaccag gaggatttaa ctttgactct
     9361 aaagtaagaa gagcatcttt tgaggtagaa gacttattcc tagcttatat tgcaggaatg
     9421 gatacatttg caaagggatt attaatagct cataaattat tagaagatga ggtatttgaa
     9481 aactttacta aagaaagata tgcaagtttt agtgaaggta taggtaagga tatagtagaa
     9541 ggtaaggttg gatttaaaga gttagaatct tatgcactgc aaatgccagt tataaagaat
     9601 aagtctggaa gacaagaaat gctagaagct atacttaaca gatatatata tgaagtagat
     9661 acaatttcta ataagtaatt gtgtagtaat taaacttgga tgtttgaata aaataacagg
     9721 atatgttctt gtttttattc taaaacggac aaagataagt atttgtaaaa attctcataa
     9781 tcatgataaa ttaaggatat agacttaagt ataattagtt tatatcctta aaatttctat
     9841 aaaaaataat catttctata gttatcaata tttttaatta tcaatacctt tctcaactaa
     9901 taaataaaaa tcagtaaagt atctaaattt aagtcattat aatggataaa ttttttagca
     9961 tatctttttt agtatatcac tgataagaat ttactttcag gatatagcag taaaatagat
    10021 atttgaatat aattgttatt ttataacttt catgaaataa ttttgaaaat acaaataaaa
    10081 atttttaaat agaataatag tggaatatat cttcaagaat ttcttttaac tgtttaggtg
    10141 ataataaaat aatgatacaa tgtattaaaa tataaaatct gaaagtggat gagaattaca
    10201 tgaaaaagaa agtattattt ttagtaatta ttttagcatt tataatctta ttaggtaatt
    10261 ctacttattc tcaaaagaat cagttgtctg aagaggaaaa ggtggatgac tttaattatg
    10321 tgtataatgt tgtaaagaca ggatatccat atctaggtgt aaataagaga cttaataata
    10381 ttgattggtt agcaaataag gatgaatata caaaacgaat aaaaaacact gaaaatgatg
    10441 aagaatttat agaagaactg tcatctatat tatctgattt aaataataaa cacacagaag
    10501 taatagataa taagaaaaga tatgaacttt ttaagaaatc atactctaat aatgattggt
    10561 atgattttct taatgataaa aaagttgtgg atagatatga ctctatgaat cttaaaataa
    10621 aaatgccaga tgatattttt gtaaaaaaag aattaatttt aaaggatgtt attaagggtc
    10681 agattggata tatgtattta ccatcaatgt catcaaagaa tggctctata aataatgatt
    10741 taaaaataat tggagattat attaacactt tagaaaaaca taaagcttta attattgata
    10801 taagaggtaa tttaggtgga agtgataggt attggcaggg aatcgtatct aaactcataa
    10861 aaaatgatgt caaaataaat ggttatagaa tttatagaaa taataatgaa atagtaaaaa
    10921 aatatacaaa tgcaagaaat ataaaattaa atcctgtaga aaatttacca gcaagtgtaa
    10981 aggaaaatgc acctaaagaa attataaaag attttaatga ttttgaagat acttcctata
    11041 ctataaaatc tacagatgat tcaaaattta agggtaatat atacttactt gttgatagaa
    11101 aaatatattc atcatcagag tcatttgcaa tgttttgtaa agaaactaaa tttgcgactt
    11161 tagttggtga aactactggc ggagatggag ggtgtataga ccctgtattg tttaatttaa
    11221 aaaatagtgg tctaatagtt agaatggcaa gttgtatgta tttaaataag aatggaatat
    11281 gtgatgagga atttaaaact attccagaat ttaaagtaaa agattgtaaa aggactgctg
    11341 attttaaaga tgataattgc ataagaaaa
//
LOCUS       DAEPOG010000124        11264 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEPOG010000124 DAEPOG010000000
VERSION     DAEPOG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.124"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            405..1055
                     /locus_tag="KR432_002680"
     CDS             405..1055
                     /locus_tag="KR432_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBH0641201.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1195..2571)
                     /locus_tag="KR432_002681"
     CDS             complement(1195..2571)
                     /locus_tag="KR432_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0641202.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2587..4500)
                     /locus_tag="KR432_002682"
     CDS             complement(2587..4500)
                     /locus_tag="KR432_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0641203.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4636..5268
                     /locus_tag="KR432_002683"
     CDS             4636..5268
                     /locus_tag="KR432_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBH0641204.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5407..5946
                     /locus_tag="KR432_002684"
     CDS             5407..5946
                     /locus_tag="KR432_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0641205.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(6122..6292)
                     /locus_tag="KR432_002685"
     CDS             complement(6122..6292)
                     /locus_tag="KR432_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0641206.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6577..7644
                     /locus_tag="KR432_002686"
     CDS             6577..7644
                     /locus_tag="KR432_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBH0641207.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7814..9715
                     /gene="cooS"
                     /locus_tag="KR432_002687"
     CDS             7814..9715
                     /gene="cooS"
                     /locus_tag="KR432_002687"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0641208.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9708..10181
                     /locus_tag="KR432_002688"
     CDS             9708..10181
                     /locus_tag="KR432_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0641209.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            10156..>11264
                     /locus_tag="KR432_002689"
     CDS             10156..>11264
                     /locus_tag="KR432_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0641210.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGV"
BASE COUNT         4306 a         1393 c         1831 g         3734 t
ORIGIN      
        1 atttaaattt atcaaataaa aaagaaagtg tctataatga ctttataaaa gttgcattaa
       61 gacactttct ttcttataat taaaaatctt gtcaccaaat ccccctaatt gttgcttaaa
      121 acttaaactg tatgtatatt tttcatttct attcgattaa attataatat acacccaaaa
      181 aaatttattt taataaattc tattgataat ataattttgt aaatatagaa ttatattaaa
      241 ataaattata tatgaaaata tatatgtata agtttattct gcgtgagtag aatgaatttg
      301 tcaagtttaa tttagttgta attgtatctt ttattaaaaa taatataaat taaaacatat
      361 aaataaaata ttttatacaa tatattgtaa aggaggtaac tttgatgaaa gcagaaaaaa
      421 caagtatata tccaattatt aaaaataatt taaacaaaat atcaattgtt gaatataaaa
      481 agggtcaaag atttgtagca tcagataaag acttacaaga agtgttcttt ataatagagg
      541 gagttgcatt agtggagtgt actactagag gaggaaataa atttcttgtt gacattgtac
      601 ctgaaaatga atttataggc aaaatcagtt acatatatga acataatttg aaatgtgata
      661 tatttgcaaa gaccaatata aaattatttc gatttgaaaa aaatgtattt gaagagtttt
      721 atctcaaacc agattttatt gcactatttc acagaaaatg tacaagaaga atatatgaat
      781 tatataaaac cagaatggta agagaactat tttcttgcac agaagttatt gcctattgta
      841 tatcaaataa acaagaaaat aatgtctgca atataaaagc tattcgtaat ttcagtgaaa
      901 tctattcaat tagtagaaaa agtagatacc attctctgct aaagttaact gaaaaggaga
      961 tagtaaaaaa aatgggtaat acatatgaaa ttttaaacta tgatgaatta cataaattgg
     1021 catttgaagt aaaagagttt ttggaagatg agtaaaaatt gttacttaat caaaataaag
     1081 tataataatt tctgtaaaaa atagaaatag tctcactgaa aatcaaaaat actttagtta
     1141 ttttcaatga gactgtttag aaatattgta tacttataat gtatagtata attattaatc
     1201 aatttccttt actatattaa tagcccattt tttagataat gctctaggaa caccaaatat
     1261 aaacttaacc acttcttcac tataagtgag taaaacgagt acaaatatag gtgcaccttt
     1321 aaatgcagct ataaaagtta gaggaataga agctaaccac atacatccta attctagaaa
     1381 tagagcatat ttagtatctc ctccacttct taaaatacca attataacca atgtattaaa
     1441 agatttaaga gccattagta ttcccatgat aaagaatatc ttagttatat cagaagctaa
     1501 gctatcagaa acactaaaca tttttaataa taatggtata tttaaaatta aaataattcc
     1561 aagtattaaa ccagctgaaa atacaagtat agaaaacttt ttagcatatt caatggctct
     1621 tttaatatgg tcagctccaa gttcatttcc aagcataata gatgcaccag atgcaataca
     1681 tacagctgtc attatgaaga aattacctgt actggttgct atctggctag ctgctatagc
     1741 agaagtacca gctcttgcat atgcaacaga atacaggaca gttccaattg cccatacaga
     1801 gtcatttaca aatataggaa agctttttga tgaaaaagat tttataaaaa ttttatcaat
     1861 agcttttaga ttttttagac caaactttaa tatatagtct tttttataaa ggtaaacata
     1921 agtaatcatt aaaattaatt cacatattct agcaataaca gtagctagag cagcaccttt
     1981 tacacccaat gcgggaagac cgaagttacc aaatataaat ccataattta aaataacatt
     2041 tgttacaagt gcaaatgcac tacaaatcat acctaactta ggatttctaa cacctcttga
     2101 accagtacta aatacagtac ttacagcaat taatggataa ctaaatactg caatactaaa
     2161 gtaatctata caaagtttta ccacctcaga atcatacgaa aatatgtgta taattggctt
     2221 tggtgttagt aaagcaggaa taacaaatac aaaacttaaa acaattgcca atacacaagt
     2281 aagacctgtg acttttctta tattactcac atccttcttt ccataaaact gtgaaataaa
     2341 aactccagca cctcctgtga taccagataa ggacatatta aaaagaaaga aaaattggtt
     2401 tgcaactcca actgaagcta cagatgtctc ccccaaacta cttatcataa ttgtatctat
     2461 tacatttaca gaagtagaaa ttaagttctg tatgataata ggtatacata aagatagaag
     2521 tattttataa aatcttttat tttcgtcttt taaagaaaat agttcttgca aataatcctc
     2581 tcctttttac ataaattctt cccactcatc atatagagag gcaaggttat tttctaaatt
     2641 aattttttct tggctaactt ctttagcttt atctggatta gagtatactt cttcttgaca
     2701 taaaagtata tctaattttt caatttcaat ctctaacttt tcaatttctt tttctatatt
     2761 ttgtcttttt attctatttt tcttttctat ttctctttgt tctctctctt ttcttttttc
     2821 atcttttatt tgagttctag ttttttcttc tttttcttct ataatactca tctcttggat
     2881 ttgccttttt ttatctatat aataatcata atttcccaga tattcagtaa ttccattttc
     2941 atctaaaact aaaattttat caacaacagt atttagaaag tatctatcat gtgatattgt
     3001 aaatatagtc ccatcataat tagttaatgc ctcttcaagg acttccttag aatcaatatc
     3061 taggtgatta gtaggctcat ctaataacaa taagtttgca tttgatagta taagtttaag
     3121 tatggcaact ctagctcttt ctccaccact caatgtagaa atcttcttaa aaacttcttc
     3181 atctacaaat aaaaatgccc ctaacatatt tctcaaagta gtttgagtaa gatgagtatt
     3241 attttcccaa atctcatcaa ttattgtgtt atctaaatta agagtttttt gttcctgatg
     3301 gaagtaggaa acatgtacat tagttccaaa tttaatattg ccactaagag gttgtagttc
     3361 attagttatt attttaaata atgtagattt gccaacacca tttgcaccta taagtgcaac
     3421 tttttcccct ctatatatat ctaaatccaa gtctttaaaa agtattcttt ctccataacc
     3481 catagataca tctctaattt gaagaacatc attaccactc tggacagaag gcgtaaattg
     3541 tatacgagct tttttcctat aagcttctgg tttatctaaa acgtctacct tgtctaatgc
     3601 tttttcttta cttcttgcac gctttaggtg tttttctcta ccataagctt ttagtctttc
     3661 aattgattct tcttgctttt ttatttcttt ttgttggtct tcaaatttct taagctctag
     3721 ttctttttct attttagata actcaacaaa tttagagtag ttaccattat atactttaag
     3781 ttttttgttg tgaacttcaa aaattctatt tacggattgg tctaagaaat atctatcatg
     3841 agatattaac atgacagttc ctttatattg ttttaaaaat acttctaacc attctattgc
     3901 ttcagagtct aagtggttag taggttcatc aagtaacaat agagttggtt tcttaagaag
     3961 taattttcct aaaagaactc tagttttttc accaccagaa agtatgctta ttggcttatc
     4021 catatcattg tcactaaatc ctaatccttt tagaactcct ttggcttcag atttatatcc
     4081 atatccattt aaatctgaaa ataattctag tttatgtgaa tattcatcca tgattttttc
     4141 tataattgga gagtttgttt ttgtactttc ttcagatatt ttaacctcca attctcttaa
     4201 ataggattcc atttctatca aatttttaaa aacttctagc acttcttcca aaatagtatt
     4261 atccgaataa aaattggtat tttgttctaa atatccgatt tcacaatctt tagaagtata
     4321 aatatctcca ctatcatatc cataaattcc tgaaattatt ttaaataaag tagtttttcc
     4381 agtaccatta acaccgatta taccaatttt atcgccttca tttactgtaa aactaatatt
     4441 ttctaatata gaatctatac caaaactttt atttaagtta ttacatgaca aaacaatcat
     4501 aaatttctac ctccttgaac tatctaaaat tataatgttt tgatataata taatcaaggt
     4561 tatatgtatt agttttgaaa aagaaatatt ttattgtgaa tcaaacaaaa aagaggacac
     4621 ttttaggagg tgaagatgtt gggaaataaa aatatatcaa tggcagttat aagaaggctc
     4681 ccaaaatatc atagatatct tggagactta ttagataggg atatacaaag aatatcttct
     4741 aaagaattga gtgatataat agggtttacc gcttctcaaa taagacaaga tttaaacaac
     4801 tttggtggat ttggacaaca aggatatggt tataatgtag aagctcttca tactgagata
     4861 ggtaaaattc ttgggttgga tcgaccatac aacgcagttc ttgtaggagc aggtaactta
     4921 ggacaagcta tagccaatta tgcaggattt agaaaagctg gattcgagat aaaagcttta
     4981 tttgatgcaa atcctagaat gataggttta aagataagag agtttgaagt attagattca
     5041 gatactttag aagactttat aaaaaacaat aatatagata ttgctgtatt atgtatacct
     5101 aaaaatggag cacaagaagt tattaataga gttgtaaaag ctggaatcaa aggtgtatgg
     5161 aattttgcac ctttagattt agaagttccg aaaggtgtta tagttgaaaa tgtaaactta
     5221 acagaaagtt tatttacctt atcgtattta atgaaagaag gaaagtagac aacttaaaca
     5281 tattaataat gagcagttgt gaagagtatt gagaaagtca gaagatttaa tattaaatcc
     5341 tctggctttt tgaaatatta ggataaaaat ttaaataatg tatggaaata aacaatatta
     5401 aggagagtgg tttttgtgga ggaattatgg aaaaaggcaa ttgaatttca tggtcatgaa
     5461 tgtccagggt tagctatagg atttaaagca gcacttgcag caagaaaata tttaggatgt
     5521 aaacagtcag gagatgagga aattgtatgt atttctgaaa atgatgcttg tggaatagat
     5581 ggtatacaag tcgtgctaag ttgcacaatg ggtaaaggaa atttactact taggatgact
     5641 gggaaatcag tatatcattt ctttaatagg gaaaatggaa aatctataag aatatatgta
     5701 aagtcaaagc catttcaaat ggatagagaa ggatacaaaa agtacttatt agaaagtgat
     5761 tttgaagaaa tatttgagat tacagatact aagttaagac ttcctgaaaa agcaagaata
     5821 tttaaatctc ttaaatgtga agtgtgtggt gaatttgcat ctgagcaata tataaggatt
     5881 gaagaaggaa agaaagtatg tcttgattgc tttaataaat atgaaagatt ttatgaggag
     5941 tattaaaatt ataaattaga ttaaagttaa aagtattaga tatgtactaa cagtatttta
     6001 agatgaatta ggtagcaaca taataaaata tataaataat attacttgaa ccatacataa
     6061 ttaaaaagag gctgattagt atttagaaaa taaaatcagt ctctttatga ataacgaatt
     6121 attattctgg ttgaggtgca tctacaggac aagctcctgc acaagaacca cagtctatac
     6181 aagaaccagc atctataaca tatgcgtcgt ctccagctga tatacagcta acagggcatt
     6241 cagcctcaca agcaccacag cttatacaag cgtcagtaat tttatatgcc attttgaaat
     6301 cctccctttt atatgtattg taattcaata acattataac aaatattatt gaatataaca
     6361 attaaattaa ttctacaaca aaaactttat tatgatattc ttgttttaaa gttttacaat
     6421 aaagtttaat ctgcttatat ttttatttat tttaaagaag tttattttaa aaataaaatt
     6481 aaatttaaaa agttatgtat tgattatatt taaaaatgtt atactctaaa aaagtatatt
     6541 ttattttaaa atttattaat acaaaggaga tttactatga aaaataaatt agtttacata
     6601 tgtattatga gtctattgct atttgtagta ggatgttcta ataatccaaa taaggaaagt
     6661 accattaaaa aagactctaa gaataaccaa agccaagtta aagaagaaaa aagagaacct
     6721 gtgaaaaaag atataactat aagtttcgct ggtgatgtaa ctatggggaa ctataaaggt
     6781 tcatcatatt atggcagttt tgaccatgaa tttaaaagac aaggagaaga ttacaattat
     6841 tttcttaaaa atgtaaaacc tgtatttgaa aaagatgact taacagtggt taatttagaa
     6901 ggtccactta ctacagcgtc aaatgcaaaa gaaaaaaaat ttgcatttaa aggaaagcct
     6961 ggatatgtaa atattttaaa atctggaagt atagaggcag taagtattgc aaataatcat
     7021 tcagaagatt attttgaaga aggaatgaaa gatacacagt ttatattgga tgagaataaa
     7081 ataaattatt ttggattagg gaaagatgct gttgtagatg tcaaaggaat taaagtgggt
     7141 ctattagggt ataatggatt atcaactgaa tataatgaaa aaaatttaaa acaaatggaa
     7201 gatgatatta agtccttaaa aaagaaatcg gatgttgttg tagtgtactt tcattggggg
     7261 atagaattag attattatcc agataaaaaa caaaaagatt ttgctcatta tgctatagac
     7321 aagggagcag atttagttat ggggtctcac ccacatgtta ttcaaggaat agaaaagtat
     7381 aaaaataagt atatagctta tagtctaggg aatttttgct ttggaggaaa taagaatcct
     7441 tctgatacag atagttatat atatcaacaa acctttactt tttcagataa taaattaact
     7501 tcaattaaag agccaaatat aataccaacc tcaataacat ctagtagctc cagaaataat
     7561 tatcaaccaa aagtattgga tggaagtgag aaggatagag ttttaaataa acttgaaaaa
     7621 ataagcaaag atttaaataa ataaaatgta aaaaaaaata aatatatata tttattttaa
     7681 taatttatat tttgtttagg gtatggtaaa tcgtggtatt aaataagtag attttaatag
     7741 ttaaattata gtcaattata ataaagctat ttaatctact tttttaatat aaaaatataa
     7801 gggggaattt gtaatggctt gtaacacttg taaaatgtgc gaaagtgcag acaagaaatt
     7861 agaaagtttt gtagcttcaa aggatgttga aacagcattt catagaacag aagatcagaa
     7921 agttaagtgt ggttttggac ttcagggggt ttgttgtaga ctctgttcaa atggaccatg
     7981 tagagtaact cctaaatcac caagaggtat atgtggagca gatgcagaca ctatagtagc
     8041 aagaaacttt ttaagagcag tagcatcagg agcagcttgt tatttacatg tggtagaaaa
     8101 tacagctaag aatttaaaaa atgttggaat aactaaaggt gttgttaaag gagaaaaaac
     8161 attaaatcaa ttagcagaga tgtttggaat agaaagtgat gaaaagtatg ataaatgtat
     8221 aaaggttgca gacaaagtta tcaacgattt gtataaatca agagatgata aaatggaatt
     8281 agtagaaaaa atagcttatg cacctagagt gaaaaagtgg aaagagttag gtataatgcc
     8341 tggtggtgca aagtcagaag tttttgatgc aatagtaaaa tcatcaacaa atttaaatag
     8401 tgatccagta gacatgctag ttaattgttt gaacttaggt atatcaacag gattatatgg
     8461 tcttacacta actaatcttt taaatgatgt tatgttggga gaaccagtta taagaatggc
     8521 tcctgtagga tttaatgtaa tagacccaga ctacataaat ataatgataa caggccatca
     8581 acattcaaca tttgcaaatt tccaagatag attaaaagat gaagatgtta taaaattagc
     8641 tcaatctgtt ggtgcaaaag gctttaagct agtaggatgt acatgtgttg gtcaagattt
     8701 acaattaaga ggagaacatt atcaagaagt atttgctgga catgcaggta acaattttac
     8761 aagtgaggct gttttatcaa ctggagcaat agatatagtt ctttcagaat ttaactgtac
     8821 tataccaggg cttgaaccaa tagcagataa atataaagta aaaatggttt gtttagatga
     8881 tgttgctaaa aaagcgaatg cagaatacat aggtttagac agaagtaaat tagatgaact
     8941 tagtaacact ttaatagaaa aagcattgga gtcttataag gagagaagag gaagtattga
     9001 gatagatata ccaaaagacc atggatttga acaatcttta actggtgtaa gtgaaaagaa
     9061 cctgaaagcg tttttgggag attcatggaa acctcttatt aatttaattg cagaaggaaa
     9121 aattaaaggt gttgcagctg ttgtaggatg ttcaaatatg acagcaggag gacatgatgt
     9181 aaatactgtt gaacttacta aagaattgat taaaaaagat ataatagtac tatctgcagg
     9241 atgttctact ggaggattag aaaatgttgg tcttatgtca cctggagcag aagaattagc
     9301 aggagaaaac ttaaaagaag tatgtaagac tttaggaata ccaccagtat taaactttgg
     9361 accatgtcta gctataggaa gactagaaat agtagcaaca gaattagcag ctgaacttgg
     9421 aattgaccta ccacaattac cattagtgct ttcagctcca caatggttgg aagaacaagc
     9481 attagctgat ggagcgtttg gtcttgccct tggcttacct cttcatttag ctttacctcc
     9541 atttataaca ggaggaaaat tggtgacaga agtattaaca gaaaaattaa aagacttaac
     9601 aggaggacat gttatagtga atccagaccc taaatcttca gctaatcaat tagaagaaat
     9661 aataatagat agaagaaaaa atttagggtt aaaggatgtg gaatgcaatg catagaattt
     9721 ttataaataa agatttatgt acaggatgta agagctgtgt actagcatgt atgctaaaac
     9781 acaataaaga ttatgacatg tacactcttg atttagaaaa tatagataat gatagtagag
     9841 ggcatataga attagatagt aaacataata atccagttcc aattctttgt agacactgtg
     9901 atgaacctga atgtgtatta gcatgtatga gtggtgcaat gcataaagat agtgaaagtg
     9961 gaatcgtttc atatgatgaa gagaaatgtg gttcatgtta tatgtgtgta atgtcttgtc
    10021 catatggatt attaaagcca gatgatagaa gcaagcaaaa catacttaaa tgtgatttat
    10081 gtaaagatga ggagtatcct agatgtgtag ctaattgccc aagtggagca attgaattac
    10141 agaaggagga aaatgatgaa ttatgtagtg ttaggggcta gtgcagctgg tataaatgca
    10201 gtaaaaacat taagagaatt agataaagat tcaaatatag tagtaatttc taaagatgaa
    10261 aacgtatatt caagatgtat gttgcatcac gttatatctg aacacagaac tttaaaacaa
    10321 ataaattttg ttgatgaaga ttttatggag caaaataatg taaaatggat tgcaggtaag
    10381 actgttaaag gtatagacat aaataaaaag gtagttcaaa cagaagacat tactgtaaat
    10441 tatgataaac ttttaatagc tacaggtgca tcttctgcta ttcctcctat aaaaaattta
    10501 agagatggaa attttgtata ttctgtgaga aacatagatg atatttataa aataaaagaa
    10561 aaagcacaaa attctaaaaa tgtagttata ataggtgctg gtttagttgg tatagatgca
    10621 ttagtgggat tatttaaata tgagcagtta aatatttctg tagcttttat ggaaaagtac
    10681 attttagata gacaattgga tgaatataca gcatcagtgt atgaaaataa gttcaaagaa
    10741 aaaggtgtaa aattttaccc aagtgcatca atacaagaaa ttgtattgga taacagtaaa
    10801 aatgttacag gtgttgcatt ttcaaatggt gaagttttag atgcagacat ggtaattgta
    10861 gctacagggg ttaaaccaaa tgctgacttt ttagatggga caggaataga atatgataga
    10921 ggtataataa tagatgatat gtgccaaaca acacaaaaag acatatatgc agcaggagat
    10981 gttgtaggta aaaatgctat ttggcctctg gcagtaaagc aaggaattgt agctgcctat
    11041 aatatggttg gaaaagataa gaagatagaa gatgaatttg cttttaaaaa tagtatgaat
    11101 ttcatggata taccaactat ttctatagga atgaatacac ctgtagatga tagctataaa
    11161 gtattaacca gacatggaat taatgattat aagaagtttg tttttaaaga taatgtaatc
    11221 tacggagcag ttatacaagg agatatttca tatgtaggtg tatt
//
LOCUS       DAEPOG010000125        10922 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEPOG010000125 DAEPOG010000000
VERSION     DAEPOG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.125"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            428..1408
                     /locus_tag="KR432_002690"
     CDS             428..1408
                     /locus_tag="KR432_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0641211.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1507..2469)
                     /locus_tag="KR432_002691"
     CDS             complement(1507..2469)
                     /locus_tag="KR432_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0641212.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2789..3739)
                     /gene="hpdA"
                     /locus_tag="KR432_002692"
     CDS             complement(2789..3739)
                     /gene="hpdA"
                     /locus_tag="KR432_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBH0641213.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3732..3989)
                     /gene="hpdC"
                     /locus_tag="KR432_002693"
     CDS             complement(3732..3989)
                     /gene="hpdC"
                     /locus_tag="KR432_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBH0641214.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3993..6701)
                     /gene="hpdB"
                     /locus_tag="KR432_002694"
     CDS             complement(3993..6701)
                     /gene="hpdB"
                     /locus_tag="KR432_002694"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBH0641215.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6998..8014)
                     /gene="tsaD"
                     /locus_tag="KR432_002695"
     CDS             complement(6998..8014)
                     /gene="tsaD"
                     /locus_tag="KR432_002695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH0641216.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(8020..8496)
                     /gene="rimI"
                     /locus_tag="KR432_002696"
     CDS             complement(8020..8496)
                     /gene="rimI"
                     /locus_tag="KR432_002696"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH0641217.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8486..9202)
                     /gene="tsaB"
                     /locus_tag="KR432_002697"
     CDS             complement(8486..9202)
                     /gene="tsaB"
                     /locus_tag="KR432_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH0641218.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(9202..9654)
                     /gene="tsaE"
                     /locus_tag="KR432_002698"
     CDS             complement(9202..9654)
                     /gene="tsaE"
                     /locus_tag="KR432_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH0641219.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9793..10833)
                     /locus_tag="KR432_002699"
     CDS             complement(9793..10833)
                     /locus_tag="KR432_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0641220.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3559 a         1943 c         1112 g         4308 t
ORIGIN      
        1 ttatttaatt attatttatt aaccagacga atgtaataat tattaaatat acaatattaa
       61 aatcaaagag aataataaat gtaatattaa aactcaataa taaatttata ttcataagtt
      121 taatttcttc attaatttta tcaactatgc ctctactgcc tattaattta atgcaataaa
      181 taataaatct tttacaaaaa gataataaaa atatagaaaa atatttttta ttgtcctttt
      241 gcttatggta aaccacattc tgcctgcaat ataactaaat atatgggtat atagtaaaat
      301 taattttatt atttatatat aattttataa gttacttttc aataatattg actgtcatga
      361 atttataata ttcaataaaa tattttaatt tagttaagaa tctatattgt aaaggagtat
      421 taaattaatg aacttaaaat taatatggat aaatattcta aacccagtat ttattaaaaa
      481 tgaaataaaa agaaaaaaaa tacaaattat tatctttttc tttataaact ttggtttaac
      541 ttattcatta ggtttactgt tatattataa taaatttatt gatccagaaa actttgcatc
      601 attcatgatg atattacctc tatcttcagt tgctattgca aagttttata ctgaaggatt
      661 aaataatgat atgtatgaat tttattcact aatcatatta ttttttctta tttattcatt
      721 attaattgtt cttaagttat taaatctaat aaataataat gaatttataa ttataaacct
      781 aatattaact tttataagta gcttatcaat tataatatat tcatcaaata aaaaaaattt
      841 atatcctgta aaaaatatta aactaggttc tttattaatt atttacttta tgatttctgt
      901 aattgttcca acatcattta tactaatact acaaggggct caacctaatt atagtggtat
      961 cataaaattt atatttactc caataacatc attaactact atttatattt tttttagtga
     1021 agaatatgcc tggagaggtt ttttacagaa tatttttttt gataaatttg gtaaaaaatt
     1081 aggagtcata atattaggta tgtgttggag cttgtggcac ttaccactaa tatttacatt
     1141 atatacacct gaagcgccaa tattaggagt aatattacgt tcaatacata tagttggaat
     1201 ttcaatattt ttaggatatc tttatataaa aactaagaat atctggtttt gtgctataat
     1261 tcatgtttta aataatagtg cttttcttat cacaaattct aaagaatcaa ctatcactta
     1321 ccatgatata ctaatagtat ctatggttgt tttaattttt tatgttccat ttctatttac
     1381 aaaagaatat aaaaataact tggagtaaat agaatatcca agttattttt atatttttgt
     1441 aaatttccaa caatcctaca gaatacttta agtttagaag atattattaa aatttattat
     1501 aatcaactaa ttcttgatat tagtcacatt tactttatca ctaacaaaat catacttaga
     1561 atctctatag taataccata caaatataaa gctaagtaga ttaacaaaag tagtcaaaat
     1621 tccaccttca ataccaaaca tccctccatt taatagatta ccatgagtga tttgtgtagt
     1681 tataatccct ggaacctgaa gtccacttac aggcatacca tatataatcc cctgaaatac
     1741 attccatgta aaatgataac ctatacacat ccatatattc gacgacttta taaacatata
     1801 tgaaaaaaga atcccaataa ttataatatt aattacagac aaaaaagtaa catcaggatt
     1861 ccatatatga atcaacccaa aaataacaga tggaacaatc attataaaat aaatattttt
     1921 acatcttcgt aaaactgcca taaaaaaacc acgatttaaa atttcttctg caaaagcaac
     1981 taaaataaag acaagtatcc aagaaactgt taaaggtgaa atttttggta cccatgaagt
     2041 aacatgacca ccaccaattg ttattgttat aataaagatt actgtacaat taaatattcc
     2101 taataacatt cctacatatc cttctttttt cttaattcta taagaatgta atcccatttc
     2161 actaatagga tttttcataa taaatctcca tacaattata ggaataataa tcataagtga
     2221 atctgttaga atttgaaata caacctgtaa cccaacgtca tttaaccaat tagcatattc
     2281 cgaaaaatag tttgttttta aattaatgtc acctgtagca atcagaattt ttcttattat
     2341 ttccataagt atagtgctta aataatactc aaatatgtaa aaggcagcca taacagttat
     2401 aataatccac cctgaacgta cttttttgtc tttattctta ataatcaagt tactccatga
     2461 tttgttcata tagatgtctt cctcctaact attattttta gttgtttcta aaatatattc
     2521 tattaaattt cttcatagta gtattttacc acaaaacttc tgcttcaata aagtaaacgt
     2581 aatatattct ctaactgtaa tttaaactat aaaaaatata taaagtataa atttaaagta
     2641 atatttatgg acataaagca atatgaacaa tatataaaac atataaaact ttacttaatt
     2701 atttctacat aaaaaccaca taaaaaagga tgtaactcta gcttttagtg taacatcctt
     2761 ttttaattaa aaaaacttgt atttgatttt aaaaagctgt ctcatgacca acatagcaag
     2821 ctataccatt atccaaaaat atatcttgta attcttcaag atgctcttca tcaatatctc
     2881 ccttatcaga atactcatac tccttaccta attgaatcca ctttgattct cctagcctat
     2941 gaaatggtaa taagttaatc tctattaaat tatttttgtg cataaaagat ataatatcac
     3001 ttatattttc agcactatca ttaaacccag agataactgg tactctaagt actaaccttc
     3061 cattccagtc agaatttgca aggtttgata tattttctaa tattaaatca ttatatacac
     3121 ctgtttgttc tttgtgtttt tctctatcca tatgctttat atcaataaat gcaaaatcaa
     3181 tatctttaaa tattttatta aaaacttcat ttgatacaca tgcacttgtt tcaatggcag
     3241 tgtgtatatt tacctcatga cactttaata atacttcatg taaaaattca tgctgaagta
     3301 gtggctcacc tccactaaat gttactcctc cattacttct ccaattgtta gaatcacgtt
     3361 taattacctg cacaagttca tctacagtat aaggttttgc acataattta aacgcattat
     3421 aataacatga gttgacgcat tcaaaacttt cacagtcctt acagatgttc caatctataa
     3481 ctggcttatt atcaagatta aagctcaatg caccattttt acacttacca tgacatacag
     3541 tacatccatt ttcatattga caagataatt cactaaacat catatgtggt cttacagtcc
     3601 aactttctgg atttgcacac catttacaac tcagtggaca tccatttaaa aatacagttg
     3661 ttctacaacc tggaccatca tgaactgaaa agctttgtac atcgaaaatc atgccttcta
     3721 attgcttttg actactcatt aaacacatgt ccttcacaag taattgcatt caatgttgaa
     3781 tatacccagt cttctttctc taggccaaca catttgccta taccttcatc attagcttca
     3841 acaaaatttt tacaattttt acattttggc attactttta tctctggaca tgctgaatca
     3901 tctaaattaa tcatttgttt tgataatcta caaattcctt tggttgcatc tactgcacaa
     3961 aaattcatac aatcactatg ctttctcata ttttacaccc cttcatactc tgttctagca
     4021 attacttcat cttgtattgg tttacctaat tcacaccaat attgagtaaa cccagcaaca
     4081 cgtaccatta attgacgata attgtcagga tttttttgtg catcttttaa tgtttctgaa
     4141 tctacaacat tatattgaat atggaaccca ccttttctta aataacttct tgttaaatct
     4201 aataattttt tagttccttg ttgtcctttt atagtagttg gatgaagttt taaattcatc
     4261 tgagaatttt gaactactgc ttggtcccaa atagttgcag attcaaataa tgcatatact
     4321 ccatttttat ctgttcctgc atatgcagag actgaaccat ctgaatatgt tgttccactt
     4381 agtctaccat ctggagtagc caaggtagca gctccctgtg gtccatgtgt agaaacagat
     4441 atttggcaag ggtacatctt cttagcatat aaagattgtg cttcttcaca tacttttgat
     4501 aaccatacct catagttttt aagaatatta tctgcataca aatcattatt tccatattta
     4561 ggtgcatcaa gacaggcctt gtaaatagca tcatatttac cagtcttatc tacttttact
     4621 tggtctgcca tagaataatt tcctacttct aatgcatctt taaatccaaa attatttaat
     4681 attgcatcct tcatttcctc tattgtaaat gctttgtcat catatacaag tttcttaagt
     4741 gctgcaaatg aattgaccat agtaacagta ccacaagttt ctacgtttaa tgttgcatta
     4801 taacgatatc ccatatttcc tatatgttga cctttgtcta aacagtctgg ctttagtagt
     4861 gaatttatta tagaagtatc aaattttctc catatatcta actcaatgtt atttgctctt
     4921 tctaaaacat ttattgcttg tttataataa tctttaaata cttctatgac ttcttcataa
     4981 gtatctaatg gcttattatg aggctcaaat acttgtatat tcatacgatg gtctttacca
     5041 ttcataagaa caagttctaa aattttggga ttagctataa agtgaactcc agagccagca
     5101 gattgacctg ctccacctgc aatagagtac gtttttccgt ttaaagttaa ctgtttccaa
     5161 caccctggag acgtttccaa acatccacct aatgcaaatg ctcttgcttc ttcaacagtc
     5221 ataccttcat cagcaaattg tttcatcata aatgttgttc catttgagtt gtttacccag
     5281 gctggataac cagaaccaag ctttgtacat tcagcagctt tcattaagaa atcttctgga
     5341 agcttttcat catatagcat agttaaacta ggttgaggag ttctacatct catacttgct
     5401 tctaataata actcttctag ttcattagct cctgtagaac catcttcttt taatccacct
     5461 atagataaag tattaaatgt attacctgat agtactcctc catttacgcc cgctgaagca
     5521 aaacagtcaa tacaagtgat ttttattcta taaagctcta ataattcgat aacctcttct
     5581 tttgtaatta gtcctttttc tatatcttgt tcataccaag gatataaaat ttgaccaaaa
     5641 cgtcctatag acataccaga tatagcatcc tcatttactg atgctatatg aattgtataa
     5701 gtcaactgaa gagcttccct aaatgtacga ggttgtttat gtgcaatatg ttctaatatc
     5761 tccactaaat ctgagtattc ttttttagct tctaaatctg tctctatact ttctaaatat
     5821 tttgcatgtt ttgcataatt taaaatccaa gtttgtaatc cttcaataac ttgaataaca
     5881 gcctcataaa aatatagacg gtccatacca ataagcccat ctccagatgc atttcctgca
     5941 actgcctttt tacattcttt tgccatttct ataattccat ctaatccata atttaaagga
     6001 taaaaataat ttataacttc tctaccttga ggaagtgtat accctgaatc aaacatgcat
     6061 ataagtgtac tcattaggtt ttcttttaaa tcataatctg gcatcatttt ttcatagtga
     6121 aaacctaaat cttcaacaga ctttccaacc cattcctttg ccatttttac aagtacaggt
     6181 acttcttctt ttctcattcc gaatttacca gctattgaaa cgacatttcc aaaactttca
     6241 gtaacatttc ctccaccaga cccaaatttt gtaagttcat cagctgtgtt actggctaat
     6301 ttctttgcct cttctctcat ttgttctcct tgagcaacaa agaaactttc acttacccat
     6361 ggcattggaa aagaacctct ataatggcgt gtcttttgca ttactatttt ctcaccaggt
     6421 attatatttg gtgtcatatg tgaaaaagca gctttaagag ctttagctct tctaactact
     6481 ggtatatcac catctgattt tctatattct ctattatacc aatatggaaa ttccatatca
     6541 gcagtagata atgtattata atatacatct aataagtctt ctgctctttt acttgctttt
     6601 ttttcattaa attcacttaa attttcttta ttttgaaaat ttgatttaat attttttgct
     6661 tccaaaatac ttcttatttt gtcttcttta ctttgactca tttcttcccc tccttaatct
     6721 ttctataatt agtataaaac tttttaagaa tgaaaaaaaa gggtatatta cccacttttt
     6781 aaaataatct ttctatagaa aaaattaaac ttgaagcaaa tctaagccaa agacaatctg
     6841 tatatcctaa aatttgttgc aattcattgg aatataaaga ttataaatag ttttacttca
     6901 agttttaata tatactatat ttatttttat tatatttaat ttcacttcta cttacttttt
     6961 tgaaacaata cttttatatt tacaaattca tctaactcta ctgatttatc tttaaatttg
     7021 gaactgcatt taaactcata tcatgcgttt tcccatttat aaaattataa tatcctgcac
     7081 accctatcat agctgcatta tctgtgcata aaattagagg aggatactta acagtaatac
     7141 cattgtcttt agcaagctct gttattttag ctctaagccc cgaattagaa gcaaccccac
     7201 cagaaagagt tatgatattg tatcctttgt cttttactgc ttttagtgct ttattagata
     7261 aaacctctac tacagcctct tggaaagatg ctgctacatc ttcaactact atttcttcat
     7321 ttttcattct ttttccattt agataattta atacagaaga ttttaatcca ctaaaactaa
     7381 aatcatagct atcttcttct aaataggctc ttggaaattc tatagcatgt ttatttccat
     7441 tcttggccag attatcaata ataggacctc ctggatagcc taaattcata gctcttgaaa
     7501 tcttatcaaa tgcttctcca gatgcatcat ctcttgtttt tcctaaaatt tcatacttgc
     7561 cataatcttt tacttcaacc aagtgagtat gacctccaga tactattaat gttataaaag
     7621 gaggttttaa atccttatgc tctatatagt ttgcacttag atgcccttct atatgattta
     7681 ctcctacaag tggaatattc aaagtatatg caagtgcttt agcataagat agaccaacca
     7741 ataaagcacc tactagccca ggtccatatg taactgcgat atggtctata tcattaaaac
     7801 ctatatttgc tttgtctaaa gcttcttgaa caactatatc aatattttcc acatgttttc
     7861 ttgaggccac ttctggaact acaccaccaa attttttatg tgtctcgatt tgagtagaga
     7921 ttatgtttga aagtacttct ctaccatttt ttaaaactga tgcagctgtt tcgtcacaac
     7981 tactctctat agctaatgtt attatatcac tcataaactc tacacctctt ttaattggtt
     8041 ccacattata attgcatctt ctttattatc actgtagtat tcttttctta ttccacccat
     8101 tttaaatcca tattttctat ataaattttg tgctatttta tttgatgctc taacttctaa
     8161 tgtcatagat gcaatattat tatctttaca taaatcaact atgcatttta tcaacttgtc
     8221 ccctattttt ttccctctat agtcactatg aacagccaca ttatttatat gcccttcatc
     8281 aagtataagc caaattccca catatccgac aatttttcca tttaacttag ctactatata
     8341 ccttgctact tcattagaca actcttttct aaaagactct tttgaccaat aatcttcaaa
     8401 acaatttttt tctacttcaa aaacttcatc tatatcttct gtagtcattt cttctatttt
     8461 tatatcatct actattagtt ttgtattatc ttccattatt tagcctcttc attttttcat
     8521 catattgtac ttcagcttga gattttctga tatacatagg atttatcgta taacaagtat
     8581 gtacatctat atttttatta tatttattta atgctaaaga gcataagctt cctgcgttag
     8641 atacattatt agctggtgaa ggtattttta tattttttat attctctaac ttgtctttat
     8701 atttgtatac agcttctcca actattataa attcttcatt agttgataca atttcatcta
     8761 ctaaagcatc aaactctaca acatcaacac catctaattc tatcatctct ccattttcaa
     8821 acttgtattt agctgaataa acttgatttt tctgcgcatc taatatacaa catatctttc
     8881 tatcacagaa atttatgtta tttgctagag attccaaaga atttactgct attattggta
     8941 tattatttac atgggccata gcttttactg tagccatacc tattctaagt cctgtaaacg
     9001 aacctggacc tatacataca gctaataaat ccatatcttt tatacttaaa tcactcatac
     9061 tcaacatatt ttctatcatt ggcataagct tttgtgaatg agtttttttg ttgtttactg
     9121 taaactcaca tattaagtta tcatcttcaa caactgctac acttgctgcc atagaagacg
     9181 tatccatacc tagtattttc attatttaat caactcctca actatctctt tataaaactc
     9241 tccttttgga gttaatatca tttctcttcc ttcatcttta tatctcaatt ctatattcaa
     9301 atattctttt ggaagtatat cttctattag attagcccat tctattatac agattccatt
     9361 tgaatttaca tattcatcat aaccaatatc atacatttca tcactactac ctattctata
     9421 aacatcaaaa tgatacaaag gtatttttcc ttcatattca tttattatag taaaagtagg
     9481 acttgttata taatcttcta tccctaatga atctgccaag ctttgtgtca ttgtagtttt
     9541 accagctcct aaatctccta ctaaacagat aacactgcct tcttttaaaa gtttacctaa
     9601 tttatatcct atttctctag ttttattttc attttctaaa tatatttttg ccatcaattc
     9661 atctccattt tgatataatc gtcatgtgaa tttatatcac tgcttaatta attatagtat
     9721 aagcctagat tattataaag tacttgtctt tatctttttt tatctttcac tattgatggc
     9781 tgtatctaca ccttatatac aatcatcaat agcaattttt acatactctt ttgctttatg
     9841 gttttgcttc aatgcttctt tacatattct ctcagattgc tcttttacat acttaagaga
     9901 cttatagttc tcttttactg cttcaataca cattttttct gtttgatttt ttatatacat
     9961 aagcattttt ccattttgtc ttacaatctc tatacatata tcttctgtat acttatctat
    10021 gtaacttagt acatctttaa tattatattt tttatttaaa tatcttatag catttataca
    10081 tatttcttct gtcttattgt tgacaaattg tagacttctt ccatcttgtt tgacagcttc
    10141 tatacatatt ttatcagttt gtttatttac atattgtaat gccataccat tttgtcttac
    10201 ggattctata cacatttttt ccgtttgatt ttttacatat tttaatgctt tataattttg
    10261 cttaatggcc tctatacata attcttcact ctgttctcta acgtatgcaa gacacctgcc
    10321 atctttttta attgcttcta aacatatcct tcttgtttga gtctctaaag cctttagtat
    10381 acttacaaca tctatattgg aagaatgact tattgctttt ataataagcc cttctgtttt
    10441 atttcgtaca tacttaagtg cattacaatc tttttttata gcttcaagac acaagtattc
    10501 tgtttgttct tttacatatt caagtgcttt ataattatac tttattgcct ctaaacataa
    10561 ttcttcggtt tgatttttta catactctaa tgccttgtaa ttctgcctta ttgctaaaat
    10621 acacatacca tcagtttgat tttctatatt ttttaattca cgataatcgt attttaccct
    10681 ttccatattt tcaatatatt cattagtgat atactttaaa gaatctccta attttgtatc
    10741 ataaatacca atatgaatat cttcccaatt actattaaaa taataccctt tatcatctaa
    10801 aaataatttt tttatttttt tagataaatg catgaatata cacccctatc attattaaat
    10861 tagttattat taaaagtttg attattagtc tttatagttt tttaataata actttatttt
    10921 tt
//
LOCUS       DAEPOG010000126        10886 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEPOG010000126 DAEPOG010000000
VERSION     DAEPOG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.126"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            134..1456
                     /gene="dnaA"
                     /locus_tag="KR432_002700"
     CDS             134..1456
                     /gene="dnaA"
                     /locus_tag="KR432_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH0641221.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            1698..2804
                     /locus_tag="KR432_002701"
     CDS             1698..2804
                     /locus_tag="KR432_002701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH0641222.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            2941..3147
                     /gene="yaaA"
                     /locus_tag="KR432_002702"
     CDS             2941..3147
                     /gene="yaaA"
                     /locus_tag="KR432_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBH0641223.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            3165..4280
                     /gene="recF"
                     /locus_tag="KR432_002703"
     CDS             3165..4280
                     /gene="recF"
                     /locus_tag="KR432_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH0641224.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            4281..6182
                     /gene="gyrB"
                     /locus_tag="KR432_002704"
     CDS             4281..6182
                     /gene="gyrB"
                     /locus_tag="KR432_002704"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH0641225.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            6202..8628
                     /gene="gyrA"
                     /locus_tag="KR432_002705"
     CDS             6202..8628
                     /gene="gyrA"
                     /locus_tag="KR432_002705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBH0641226.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            8697..8993
                     /locus_tag="KR432_002706"
     CDS             8697..8993
                     /locus_tag="KR432_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0641227.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            8993..9253
                     /locus_tag="KR432_002707"
     CDS             8993..9253
                     /locus_tag="KR432_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBH0641228.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            9265..9582
                     /locus_tag="KR432_002708"
     CDS             9265..9582
                     /locus_tag="KR432_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBH0641229.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            9607..9993
                     /locus_tag="KR432_002709"
     CDS             9607..9993
                     /locus_tag="KR432_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0641230.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            9993..10766
                     /locus_tag="KR432_002710"
     CDS             9993..10766
                     /locus_tag="KR432_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBH0641231.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
BASE COUNT         4452 a         1127 c         1993 g         3314 t
ORIGIN      
        1 aataatttat actattatta atttatcaac accattatcc ataaatttag ttattctgta
       61 aaatttatat atatttttgc ttttctgtta ataactttgt ggaaaatatt aattattctg
      121 ttcggaggtt tatattatgg atatagtttc tttatgggac aaaaccctac aattaataaa
      181 aggtgactta acttcagtaa gttttaatac cttttttaaa aacatcgtac cattaaaaat
      241 acatcttaat gatttaatct tactggctcc tagtgatttt aataaagata tcttagagaa
      301 tagatattta catttaattg aagatgctat tagtcaactg tcattaaaaa aatataatat
      361 taaatttgta ttgagcgaaa aagaggttgc tgatttaaat tctgatagca cagatttaaa
      421 ttatagagtt ctttacccca atctaaatcc aaaatacaca tttgatacat tcgttattgg
      481 taatagcaat agatttgcac atgctgcatg cgttgcagtt gcagaatctc cagctaaagc
      541 ttataaccca ctttttttgt atggaggagt tggattaggt aagactcacc taatgcatgc
      601 tataggtcat catattgtta gtcaaaaaaa agactcaaaa gtagtttacg tttcttcaga
      661 aaaatttact aatgaactta taaactctat aaaagacgat aaaaacgaag aatttaggaa
      721 taaatataga aacgtagatg tattactaat cgacgatatt caatttatag caggaaaaga
      781 aagaactcaa gaagagtttt tccatacatt caatactctt catgaagcaa acaaacaaat
      841 aattatatct agtgatagac cgccaaaaga catacctaca ctagaagata ggctaagatc
      901 gagatttgaa atgggactta taactgatat tcaagcacct gactttgaga caagaattgc
      961 tatacttaga aaaaaggctc aactagaacg tatagatgtg ccaaatgagg ttatgtcata
     1021 tatagcaaaa aatattaagt caaacataag ggaactagaa ggtgcattaa ctagagtggt
     1081 tgcttattct tctcttagca atagggtaat atcttttgat cttgctacag aagctttaaa
     1141 agatattata acaacttcta aaaatgaaga aataaatgtt cttagaataa aagaaaaagt
     1201 atcttctgta ttcaatttaa agatggaaga ttttaattct aaaaaaagaa cacgttcaat
     1261 agcttatcca agacagatag ctatgtattt aacgcgcgag cttactgatt tatcccttcc
     1321 taaaataggt gaagagtttg ggggtagaga ccatacaaca gtaattcatg ctcatgacaa
     1381 agtatctaag gatatagaag aaagtgaaga aattaagact aaaatagaca aaattatatc
     1441 agatttgaag ggataattta tacacaattg tatgtttata tctcttgaat aacctgttaa
     1501 tacttttttt gaacaaaaat tattaacaaa ttatcaagtc taaaataaca aagatattaa
     1561 catcttatac acatgttaat atctttgtat ttataatgtt atacatactt attaacatta
     1621 tcaacaacac ctactactat tactactatg attttaactt atttttatat ataaaaagcc
     1681 agaggaggtt atccacattg aaaataattt gtaatcaaaa aatcctagca aatagaattg
     1741 gaattgctca aaaagctatt aatggaaaaa caacaataga attattaaaa ggtatactta
     1801 tatctacaga agaaggtcaa ttaaaactta ctggatatga tgctgaaata ggaatagaaa
     1861 cttatgtaca agcagaaatc atagaaaaag gtgatgttgt tgtagatgca aggctatttg
     1921 gagatataat tagaaaatta ccagattcct ttgttgagat agaaactgat tcagaaaata
     1981 atatttacat aaattgtgta aattcaagat ttaaaattaa aggttatgca gcaaaagaat
     2041 ttcctaaatt accagaatta aatgaagaag atttatatag catacctcaa gaaattctaa
     2101 aaaatatgat taaacaaact gtatttgcta tctcacagga ccaaacaaaa cctgttttaa
     2161 tgggagaact tttagaaatt gtagatagaa acttaaattt agtagcaatt gatggatata
     2221 gattagctgt aaaaagttgt tcagttgata gtttaactga aaatataaaa gtcataattc
     2281 ctggaaagac acttatagat gtaaatagtt tgctttctgg agaggataat gttaaagttg
     2341 ggtttaacga aaaaaatgct atttttataa ttaatgatac gaaaattatt acaagattgc
     2401 ttgaaggaga ttttattgat tataaaaaat tattgccaag agaacataac agcagagtta
     2461 aattaaacac taaggaactt ttaaatagta tagagagagc gtctttattg tctcaatcag
     2521 aaaaaaataa tcttataaag ttatctataa gagataaagt aatggcaatt acttctaaca
     2581 cagaaaaagg aaatgtgtat gaagaagttg aaatagattt agatggagat tacctagata
     2641 tagctttcaa ttctagatat tttatcgaag gtttgaaaaa tatagataat gaggaaatat
     2701 ttatagaatt tactacaaat gtaaatcctt gtataattaa accaacagat gatgttaatt
     2761 atatttactt actgcttcca gtaagaatat catcaaatat atagcagaca tgaaggagtt
     2821 gtcttttgta gaaaattttt ctaatcttag acaactcctt ttataagcat atttatggta
     2881 taatatactt ttagtgtatc aataggaatt tgtttaaaat agtgacaagg aggaactatt
     2941 atgactgaaa taactataga atcagaatat ataaagttgg atcaatttct taaattagca
     3001 gagattgcat caacaggagg tcatgcaaaa tttttaattc aagaaggttt agtgacagta
     3061 aatgatgaaa tagaattaag aagaggaaaa aagataaaat caggagatat agttaaaata
     3121 gaaggaacta aaataaaagt attgtaaaaa aaggagaact ttttgtgaaa cttaaaagtt
     3181 tgcaacttgt taattttaga aattataaaa agttgcatct ggaatttaat gggaaagtaa
     3241 atctacttgt tggtaaaaat ggtcaaggaa agacaaatat agtagaatct atttatatgc
     3301 tttcttttgg aaaatcattt aggactaaca aagataaaga aatggttaga tttaatagtg
     3361 aaaacttata cattggagga agtttttcaa aatataataa atatagtcta atagagctaa
     3421 ttattggaaa agataaaaag gggataagaa taaataaagt tcctttacaa aagatacaag
     3481 aactacttgg caacttaaat gttgttatat tttctcctga agatttaagg ttggttaaag
     3541 agggtcctaa agaaagaagg gcatttatag acaaagagat tagtcagatt atacctaaat
     3601 attataaata tcttaccaat tataataaaa cactttctca aaggagtagg gtgcttaaaa
     3661 atatacatgt tgatgaagct ttattagatg tatatgatga tacgttagca aagtatggaa
     3721 gttatattta tattttgcgt agagacttta taaaaaagat agctaatata tctgaaaata
     3781 tgcatatgaa tttgactaat ggagttgaaa gattatcaat tcgatataaa aatcaaataa
     3841 atataactga tgaagatact atagatacag tatataataa atttttagcc aagttgtcat
     3901 cgaatagacc taatgatata gaatctaaga ctaccagata tggtatacac aaagatgact
     3961 taaatatatt cataaatgat ttagatgcaa gactttttgg ttcacaaggt caacaaagaa
     4021 ctgcttcaat atcattaaag ttatcagaga tagaattaat aaaaaatgaa gttgaagaat
     4081 atccagtatt aattttagat gatgttttta gtgagttaga tgaagcaaga caaaaacttt
     4141 tagtaaataa tttaagcaat gttcaaatgt ttataacaag tgctgaagtt tcacataaaa
     4201 aaatatttga tgaaaaaaat gttacaatat ttaatattga aaatggcgat gttattagca
     4261 tagagaatgg aggaaattaa atgaaacaag aatacggtgc aagtcagata caagtattag
     4321 agggattaga agccgttaga aaaagaccag gtatgtatat tggtagtaca agccctagag
     4381 gtttacatca cttagtttat gaagttgtag ataatagtat agatgaggct ttacaaggat
     4441 attgttcaga catatatgta tctattaatg aagatggcag tgttttagta aaagataacg
     4501 gaagaggtat acctgttgaa atacatccta aaactggtaa atcaacttta gaaacagtat
     4561 taactaatct tcatgcagga ggaaagtttg gaggcggagg atataaagta tctggaggtc
     4621 ttcatggagt tggtgtttct gtagttaatg cattgtcaaa atggatggta gcagaggttt
     4681 atttaaacgg aaagatatat aagcagacat atgagaaagg attaccaaca tctaagcttg
     4741 aagtagttgg agaatctcaa gataaaggaa caatgattca atttatgcca gatgagacaa
     4801 tatttgatga aatagagttt aaatatgaaa cattggagta taggcttaga gaactttctt
     4861 tcttgaataa aggaataaaa atagtatttg aagataaaag agaaggacaa gaaaaaagaa
     4921 aagaatttca ctatacaggt ggtttggtag aatatattaa gtatttaaat aaatctagaa
     4981 ctggtataca tgatgacata gtttatatag ataaaaaggt tgatgattgt tttgttgaac
     5041 tagctatgca atatacagat ggttatacag agaacatata ttcttttgca aacaatataa
     5101 atacacacga aggtggttct catttaagtg gatttaaagc agctcttact aaaactgtaa
     5161 atgattatgc taagagaaat aaatttttaa aggaaaatga tgttaaccta cttggtgaag
     5221 acataagaga aggtcttaca gcagtagtat cagttaaatt accagaacct caatttgaag
     5281 gtcaaactaa aacaaagcta ggtaatagct ttatgagagg tatagttgat agtgtaacag
     5341 ttgatgaact ggggtctttc ctagaagaaa acccatcaac agcaagaatc atagttgata
     5401 aagctttaag agcacaaaga gctagagaag ctgcaaaaaa agcaagagaa ttaacaagga
     5461 gaaaaagtgt attagaaagt acatctttac ctggaaaact tgcagattgt gcagaaaaag
     5521 atccatctaa aagtgaaata ttcttagtcg aaggggattc agcgggaggt tcagctaaac
     5581 aaggtagaga tagaaatagt caagctatac ttccattaag agggaaaata cttaatgttg
     5641 agaaatcaag actagatagg atactatctt cagatgaaat aaaaaatatg ataacagctt
     5701 atggttgtgg tattggagaa gattttgata tagataaggc taggtatcat aaaattataa
     5761 ttatgaccga tgctgatgta gatggagccc atataagaac tttattatta acattcttct
     5821 ttagatatat gagacctctt atagatgagg ggtatgttta tgcagcacag ccacctttat
     5881 ataaagtgac aaagcaaaag aaggaacatt atgtttactc agataaagag ttaaatatct
     5941 tattagatga aattggaaga aatggtgttg aacttcaaag gtacaaaggt cttggagaga
     6001 tgaatgcaga gcaattatgg gaaacaacta tgaatcctga gacaagaact ttattacaag
     6061 ttactgtaga agatgctgca attgcagatg aagtattttc aatgcttatg ggtgataagg
     6121 ttgctccaag aaaagaattt atagaagaaa atgcaagatt tgttagaaat ttagatatat
     6181 agagagaggg gattagtata catggaagaa aataacaaaa tactccctat tgaaatagcg
     6241 gaagaaatga aaaaatcgta tattgattat tcaatgagtg ttatagctgg acgtgctctt
     6301 cctgatgtta gagatggttt aaagccagtt catagaagaa tattatattc aatgagtgag
     6361 ttaaatttaa ctccagataa accatacagg aagtcagctc gtattgttgg ggacgtttta
     6421 ggtaagtacc atcctcatgg agatattgct gtttattatg ctatggtaag aatggcacaa
     6481 gatttttcaa ctagagcact tttagtagat ggtcatggta actttggttc tgttgatggg
     6541 gattcaccag ctgctatgcg ttatacagaa gctaaaatga gtaaattatc attagaacta
     6601 ctgagagata ttgaaaagga aactgtagac tttaaaccaa actttgatga gtcgttaaaa
     6661 gagccttcag tattgccagc tagatatcct aatttattag taaatggctc aaatggtata
     6721 gctgttggta tggcaacttc aatacctcca cataatttag cagaagtaat tgatgcaact
     6781 gtatatttga tagataatcc agagtgtagt gtagatgatt taataaaatt tgttcaagga
     6841 ccagatttcc ctacagctgc aattataatg ggaaaagaaa gtatagcaga agcatacaga
     6901 actggaagag gaaaagttaa agttaggtct agagctttta tagaagaact accaaaagga
     6961 aaacagcaaa taatagttac agaaatacct tatcaagtaa ataaggctaa actggttgaa
     7021 agaatagcag agttagttaa agaaaagaga atagaaggta tatcagacct aagagacgaa
     7081 agtaatagaa atggtatgag aattgttata gaattaaaga gggatgctaa tgctaatata
     7141 gtattaaata atttgtataa acattctcaa atggaagata cttttagtat aataatgctt
     7201 gcacttgtag atggtcagcc aagagtttta aatcttaaac aaatattata tcattatatt
     7261 aaacatcaag aagatgttgt tacaagaaga actaaatttg aactaaataa agctgaagca
     7321 agagcacata ttttagaagg attaaagatt gctttagata atatagatgc tgttataagc
     7381 ttgataagag cttcaaagac tgggcaagaa gctaagatag gtttaataga aaaattcaaa
     7441 ttaactgaaa tccaagcaca agctatatta gatatgagac ttcaaagact tacaggttta
     7501 gaaagagata agatagaagc tgaatatgaa gatttaatca agaagataaa tagattaaaa
     7561 gaaattttag ctgatgaaag attactttta aatgtaataa agaatgaaat tacaataata
     7621 aaagaaaatt actctgatga gagaagaaca gaaataagac atgctgaagg cgaaatagat
     7681 atgagagatc ttataagtga tgaagaaata gcaataactc ttactcactt tggatatata
     7741 aaaaggcttc catctgatac ttataagagt caaaaaaggg gtggaagagg tatttcagca
     7801 cttacaacta gagaagaaga ttttgtaagg cacttggtaa ctacaactac tcatagtaga
     7861 ctattattct ttacaaataa aggtagagta tttaaattaa atgcatatga aataccagaa
     7921 ggtaagagac aggctaaagg tactgctata gtgaatttac ttcaattgtc agcagatgag
     7981 aaaattgcta ctctaatacc tattgatggt aatgatgaaa atgaatattt attacttgca
     8041 acgaaaaaag gtattgttaa aaagactaag agagaagagt tcaaaaatat aaataaatct
     8101 ggtcttattg ctataggttt aagagatgat gatgagctta ttggagtaga acttacagat
     8161 ggaaaacaag aagtactttt agtaactaaa gaaggtatgt ctataagatt tgatgaaaat
     8221 gatataagat atatgggtag aacagcaatg ggtgtaaaag gtataacttt aagtaaagaa
     8281 gattttgttg tgtctatgaa cctttgcagt aaaggtacag atgtgttagt tgtaagtaag
     8341 aatggttttg gaaagagaac aaatatagaa gagtatagaa gtcaaataag agctggtaaa
     8401 ggaattaaaa cttataatat atctgaaaaa actggtacaa ttgtaggtgc agatatggtc
     8461 aacgaagatg atgagataat gattataaat tctgatggag ttcttattag aataagagtc
     8521 aatgaaatat cactgtttgg aagagttaca agtggtgtta aattaatgaa gacgaatgat
     8581 gaagtcaatg tagtttcgat tgccaaaata aatattgaag aagaatagga taaatatcct
     8641 attctttttg tataatgtag ttagataaaa atttaaaatt gaaaggagta attgttatgt
     8701 gcaataaaag aagttgtaaa aaaggtggat ttttattatt aggagctatt ttagggttta
     8761 tatttggtat gttttttgca cctaaaaaag gttctgaact aagaaaagaa acaaaagaga
     8821 agtttaatga tgtcaaagaa aatccaaaag aagttctaca tgaaacattt aatgatgtta
     8881 aagagagaat aataaattta gtggacgatg ataataatga agaagatata aagatttcag
     8941 aagaagatat agtaataagt aaaagctttg atgatgaggg agatgttaac taatgaatgc
     9001 atgggggtgg caaattggag cagtgttagt aggaagctct gctcttatag tagcaattta
     9061 tttagctaaa actctaaaca gcataaataa ggttgttgaa aaggcatata agattgtaga
     9121 ttataatgaa agacatatac aagatattgt tgaaaatgca tcatcaatat caaagggaat
     9181 agatgatata gtttatgtaa ttaataaaat attgagtata ggaaatatat ttaaaattat
     9241 taaaaggaaa taggaggttt ttacatgtct atgaatattg attcaaattt agattcacaa
     9301 aataaatttt ggaatgtttg tcttgatgga gaacttgatg tttctacagc agataaacta
     9361 aaggaacatt tacatgcttt aatagaaaaa aatatgttag atgtaaagat taatcttaaa
     9421 gatttagatt atatagactc aacaggctta ggagctatga taggtgtatt aaaaaaatta
     9481 aaaataaatg aaaaagagat atacatagta aatcctaaga gtaacgtaaa aaagattttt
     9541 actataacag gtctagataa aatatttaaa gtggagggat aaactttggc ttgtgagact
     9601 ataaagatgg aaattactac aaatccagat tatgttagta taataagatt aacagcatct
     9661 ggaattgcca ataagatggg ttttccaata gatgatatag aagatataaa ggtagcagta
     9721 tctgaagcat gtacaaatgc aataaaacac agtaaagatg atgtgtttaa tatagtattt
     9781 aatattttag ataatgcaat taatatagaa attcaagata atggaaaagg atatgatgtt
     9841 agctcaataa gtacaccaga tttagagaat ccaaaagaga gtggtcttgg attgtttata
     9901 attaaaactt taatggatga tgtagatatt gaatcagaac ataatcaagg gacaaaaata
     9961 aaaatgacta aatatttagg agttgatatt taatgaaaaa tgtagctaat gctacacatt
    10021 acctaaatat ggatacaaaa gagctattta atttatataa taaaaataaa aatgtagaca
    10081 ttagaaatat tcttattgaa agacatctgt atttagcaag attgttagct aagaagtata
    10141 taaacaaagg tgttgatttt gaagatatat atcaagttgc ttcattggca ttaatttatg
    10201 ctattgatag atatgatgtt gagaaaggat ttgagttctc cagctttgcg acaccaacta
    10261 tagtaggaga aataaaaaag tattttagag ataaggtatg gacacttaga gtcccaagaa
    10321 gaatacagga attaagcaaa aagataagtg atgcgaagat taaattagaa caagaaaata
    10381 agaagcatcc aaaggtaaaa gatatagctg attatatagg cgttagtgaa gaagatgttt
    10441 tagaggctat ggaggcttct tatggatatc aaccaatgtc tttggattca tcaagtaatg
    10501 atgattcaga ggataaagat ataactctta ttgataagat tggaaaagaa gaaggtaact
    10561 ttggaagtat agagtatgaa gattttatta ataagtttat tgaaacttta aatgaattag
    10621 aagttaaaat ttttaaagat agatttttct ttgataaaac acaatctagt atagctaaag
    10681 aactagggat atcacagatg actgtttcta gactagagaa aaaggtagta gagaagctga
    10741 aaaaagaata tgaaaaaaat ttataaaaaa atataaaaaa agtattgacc tactgtttta
    10801 aagatggtat agtattactt gtccttgaaa aaaggaacaa gaaataaaca cgaacattga
    10861 aaattaaaca gtaggttaat ttatat
//
LOCUS       DAEPOG010000127        10877 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEPOG010000127 DAEPOG010000000
VERSION     DAEPOG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.127"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            608..1516
                     /gene="trxB"
                     /locus_tag="KR432_002711"
     CDS             608..1516
                     /gene="trxB"
                     /locus_tag="KR432_002711"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0641232.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1606..3429)
                     /gene="typA"
                     /locus_tag="KR432_002712"
     CDS             complement(1606..3429)
                     /gene="typA"
                     /locus_tag="KR432_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBH0641233.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3641..3883
                     /locus_tag="KR432_002713"
     CDS             3641..3883
                     /locus_tag="KR432_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641234.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4031..4222
                     /locus_tag="KR432_002714"
     CDS             4031..4222
                     /locus_tag="KR432_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBH0641235.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4513..7014
                     /locus_tag="KR432_002715"
     CDS             4513..7014
                     /locus_tag="KR432_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBH0641236.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7174..7842
                     /locus_tag="KR432_002716"
     CDS             7174..7842
                     /locus_tag="KR432_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641237.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7826..9103
                     /locus_tag="KR432_002717"
     CDS             7826..9103
                     /locus_tag="KR432_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0641238.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9504..9725
                     /locus_tag="KR432_002718"
     CDS             9504..9725
                     /locus_tag="KR432_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641239.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(10014..10778)
                     /locus_tag="KR432_002719"
     CDS             complement(10014..10778)
                     /locus_tag="KR432_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0641240.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
BASE COUNT         4297 a         1258 c         1759 g         3563 t
ORIGIN      
        1 gtaatcaaaa gataactatt aaatatctgt aatattacaa ttttatacta cactatatta
       61 caaaaaaagt ttacaaaact ttaatttgaa aaatgtaatt atatggtata tcatataaat
      121 ataagtaaat agtgctaata tttaaactat taatcataat tggttcaaca aaagtatata
      181 gttaataatg tttaatttga agaaattgtt agtgtgaaat tacatatata ttcaaattaa
      241 attcataaat atttcacata ataatagtat actgtaaaca aaaggaggta ttaaatatgg
      301 gaatagtact tataacagca gcattagttt tggttatgta tggactttca attctatcat
      361 atatatcaga aagcaatata ttagaagaca ctgtaaatga taaccaataa ggttttgaat
      421 tatataaata agtgagaact gtaaataaaa gttaattttt atttgcagtt tttttatata
      481 tatgtaaaat aaagttaatt aataaaaaat aatgttttgt ttgtagtata tatggataat
      541 aatagtaaag tataagaatg atgagaataa tatataagaa ataataatat aaagagaggt
      601 aaacattatg gtagatatca ttgtaattgg tgctggacca gcaggtttaa cttcagcaat
      661 ttatgctatg agagcagggt taagcgttac agtatttgaa aaaaatattt atggaggtca
      721 agttgcaagt acctctgagg tagaaaatta tccagcagtt caaaagattt ctggggtaga
      781 attttctaat aatatataca atcaagcagt tgctcaaggt gttgatattc agtttgatga
      841 ggttgaagag ataaatttag agggaaaagt aaaggttgta aaaacttctt caggtgaaca
      901 taaagcaaag gctgttattc ttgcaaatgg agttgaaaga cgtaaattag gttgtgcagg
      961 ggaacaagaa tttacaggta gaggagtatc ttactgtgca acatgtgatg gagccttttt
     1021 taaagataaa gaagttgcta tagtaggtgg aggaaatact gcactagaag atgctttatt
     1081 tttagctaat aattgtacaa aagtgtattt aattcacaga agagatagtt ttagaggtga
     1141 agaagtgttg gagaaatctg tgaaagcaag agagaatatt gagatacttt acagtcatgg
     1201 tgtagaaaaa atagaaggag aaaagactgt atcgaaaata gaagtaaaaa acttaaagac
     1261 tgaagaaaaa agaactatag atgttagtgg tatttttatt gctataggtc ttaaacctaa
     1321 taataaaatg tttgaaaatg ttcttgattt ggatgaaggt ggatatataa tttcagatga
     1381 gtcttgtaca acatcagtag aaggtgtata tgtagcagga gatagtagaa ctaagttttt
     1441 acgtcaaata ataacagcag catcagatgg agctattgca gccgttcaag cagctaatta
     1501 tataaatgtt gaataatata aatttcaaat aaaaatagcc atataaactg aaataatttt
     1561 tcagtactat atggctattt tgttaattag aggtaaatgt acgttttatt taccttgtct
     1621 agatttgtta tatcttaatc tatcatgttc atttaagaat ctttttctaa gtcttataga
     1681 atcaggtgta atttcaacta attcatcatc ttctataaac tctagtgctt cttcaagagt
     1741 aaatattcta ggaggagata attttatagc atcatctgaa ccagaagcac gtacgttact
     1801 cattttcttg tttttacaag ggttgactac catatcatct tttctggaat tcataccaat
     1861 tatcatacct tcgtaaactt ctacaccagg gtctacaaac attacagctc tatcacttaa
     1921 ggcattaaga gaatacccca tagtaacacc aggtccttgt gctattaaaa caccatttcc
     1981 tcttgaaggt atttctcctt taaattcttc aaatttttca aatgaacgaa ctaaagtacc
     2041 ttctccacga gtagcgttta taaattcact tcgatatcct aaaagaccac gagtaggtgc
     2101 taagaactct atttttacat agtctccttc tattgacata gactccatca taccttttct
     2161 catgtttaat tcatttataa tagtaccaga gtaaacttca ggacagttta caacaactct
     2221 ttctattggt tccattagtt ttccatcttc tttatgcatt aaaacttcag gtttagaaac
     2281 accaacttca taaccttcac gtctcatatt ttcaagtagt atagataagt gaagctctcc
     2341 acgtccagaa actttgtatc catcagtagt atcaagaggt tcaactttaa gaccaacatt
     2401 tacttcaagt tccttttcaa gtctatcttt caaatgtcta gttgtaacga attttccact
     2461 ttttccaacg aatggagagt cgtttactaa gaagttcata gaaagagtag gctcttctat
     2521 ttttatcatt tccataggta gtggactatc taaatcgcat atagtttcac ctatagatat
     2581 atcaggaata ccagctataa caactatttc tccactagta gcttcatcaa cttcaacttg
     2641 ttttaaacct tcatacacag aaagttttga tacttttcct ttagatacaa cagaatcttc
     2701 tctacatata gcaacttgtg tattgttttt aagagtacct ttgtaaattc tacctatacc
     2761 aagtctacct acataatcat catatgcaag agctgatatt tggaattgta gaggttcatt
     2821 atcgtaatta ggataagctt caacgtgatt tacaatagtt ttaaatagag gagaaaggtc
     2881 ttcactatca tcatccattt caagtttagc aataccttgt ttagcaatac catatattat
     2941 aggaaattcg cattgctcat ctgttgcatt taagtctaca aataaatcga aaacttcatt
     3001 aactacttct tccgctctct ggtctttttt atcaatcttg tttataaata atattggttt
     3061 taaaccgaac tctaaagatt tttgtaaaac gaatctagtt tgaggcattg ggccttcact
     3121 agcatcaact aatagaataa ctgtgtcaac agttttcata acacgttcaa cttcagaaga
     3181 aaagtcactg tgtcctggag tatcaactat gtttattttg taatcttcat agtttattgc
     3241 acagtttttt gaatatatag ttatgcctct ttctttttca aggtcgttac tgtccataac
     3301 gcagtcttta actacttcgt tctttctaaa aacaccgctt tgagaaagga aagcatcaac
     3361 caatgttgat tttccagcgt caacgtgggc tataactgcg atatttataa ttttatgttt
     3421 ttgtgacatt tttgttgctc ccttcgatta aaataactta ttaattatag cacaatatat
     3481 tattaggtac aaggaggaat atattaaatt gtattaatat agtttaaatt ggataaaact
     3541 gttagttttg catctgaaaa ttattctgtt taaaaaaatt ttttagacaa ataataatct
     3601 taatagataa aaagattaat aaaagagtaa aggatgatat atgttggaag taaccaaaat
     3661 aataattaaa tattttgcaa gtgaaagtga tacttttata aaagaagaag tttgtaattt
     3721 tgaagaactt cttaagtggt ctcataaatt tattgaaggt aaaaagatag aagatgtttt
     3781 gtatataaag tctaaagaaa actataaagt aaatgtgatt aagtctttag aaattaagga
     3841 attggaaata gtgagaaaac aacagatacc accgtacatg tagtattaat aatatataaa
     3901 attaatatgt aatactcaca ataggatata aatggtatat atggtaataa tttatattaa
     3961 aaaaactttt ataaagttta tatttgggta taaaagtatt aattagacat taattaggag
     4021 gaacttaaat atgataagag tatgtccaaa ctgttcaaat gtggatgtaa ataagctaaa
     4081 aactttggtt ggagaagaaa atgtaaagac tggttgtata ggtcagtgta gagcatttaa
     4141 aaaagaagct gttggaataa tagacaatga gcttgaaata aaggaaacag aagcagagtt
     4201 ttttgaagca tgtaaagaat aaattttaaa taaaatataa attataataa atagagcatc
     4261 taaattgtat aagcattaaa caatttagat gctctatttt tatatataca tttaattatg
     4321 agtatacttt tctattaaat acaaataaat ataactaata catttattta ttaaaaaaat
     4381 attgatttaa tatacctata gggggtataa taaaaatata gatttctaaa taagtaattt
     4441 atcaatatta catatcaaaa taaaacgtta caaattagct gaaaatatat tgaaagaaag
     4501 gaagatttta ttatgagttc aaataaagtg gtagaaagtt ataaaattac aggtatgaca
     4561 tgtgcagctt gtgcaaaggc agtagaaaga gtaactaaga agatggatgg agtatatgac
     4621 caaagtgtaa atatagctac tgaaaaactt aaaatagaat atgataattc taaggttaat
     4681 tttgatgata taaagcaggt tgtagaaaaa gcaggatatg gaattataaa ggaagaaagt
     4741 aataaaaaaa ttgatatgaa aattgatgga atgacatgtg cagcttgtgc aaaagctgta
     4801 gagagagtgg taaaaaaatt ggatggagta gaaagtatta gtgtgaatat tgcaacagat
     4861 aaagctaata tagactatga cccatcaaaa gtaaaattat ctcaaataaa agcagctatt
     4921 gaaaaagctg gatataaacc aatagaagag gtaagaaata aggttgatgt ggatgaagat
     4981 aaattgagaa aagaaagaga aatgaagtct ttatttgtta aatttatagt tgcaatagta
     5041 tttgcagtac ctctgtttta tatagcaatg ggacctatga taataaagcc tatagggcca
     5101 tggcctttgc cagaaattat aaatcctatg acaaatactt ttaattatgc attaattcag
     5161 ttaattttag taataccagt aatgatagct ggttataaat tttatataaa tgggtttaaa
     5221 tcactttttt ctttaagccc taatatggat tctttagttg caataggaac attagccgca
     5281 tttttatata gtctatatac gacactacaa atagccaatg gacaaattca aggtatgcac
     5341 catcatcagt tgtattatga aagtgcagga ataataatag cacttatctt acttggtaaa
     5401 tatttagaat caaaatcaaa aggaaaaaca tcagaagcaa ttaaaaagct tatggggcta
     5461 caacctaaaa ctgcaatagt gttagttgat ggtaaagaag ttgaaactcc tatagaagaa
     5521 gtagagatag gagatatact attagtaaaa ccaggaacta aaatacccgt ggatggagtt
     5581 gtaatagaag gatatacatc agttgatgaa tcaatgctta caggtgaaag tataccagta
     5641 gaaaaaaatg ttggaagtaa ggtgacagga gctagtatta ataaaaatgg tgttattaag
     5701 tttaaggctg aaaaaattgg tggagatact gctcttgccc aaataataaa gttagttgaa
     5761 gatgcacaag gtacaaaagc acctattgct aaacttgccg atactgtatc tggatatttt
     5821 gtaccaatag taattgcaat agctgtagtt gcatcattat tgtggttttt aataggagga
     5881 aaagatatag tatttgtact tactatattt atatcagtac ttgtaattgc ttgtccttgt
     5941 gcattaggat tagcaacacc aactgctata atggttggga ctggaaaggg cgcagaaaat
     6001 ggaatcttga taaaaggtgg agaggcacta gaatctgcac ataaagtaaa tacagtgata
     6061 tttgataaga caggtactat aacagaagga aagccaaaag taacagatat agtattaaat
     6121 aataatgtaa aagaagaata tcttatcaag attgcttcaa gtgcagaaaa aggttcagag
     6181 catcctcttg gagaagcaat agttaagtat ggagaagaga agaatataaa atttgaaaaa
     6241 gttgataatt ttaaagccat accaggtgct ggtatacaag ttactattaa tgatgaaagt
     6301 atattacttg gaaatagaaa gcttatgaat gacaataata taaagcttgg ggatttagaa
     6361 gaaaaatcaa atgtattggc aagccaaggt aaaacaccta tgtatatagc tgtagatgga
     6421 aatttatcag gtataatagc agttgcagat gtagtcaaag aaagtagtaa aaaagcaata
     6481 gaaatacttc atgatatggg aataaaagtt gcaatggtaa ctggagataa tgcgaaaact
     6541 gcaaatgcta tagcaaatca agtaggtata gatatggttt tggcagaggt tttaccagaa
     6601 gataagtcaa aagaagttga aaaactacaa aatcaaggaa agtttgttgc aatggtaggg
     6661 gatggaataa atgatgcccc agctcttgca aaagcagata tagggatagc aataggaagt
     6721 ggaacggatg tagccattga atctgcggat atagtactta tgaagagtga tttaattgat
     6781 gttccaacag caattaaact tagtcatgaa actataaaaa atataaaaca aaacctattt
     6841 tgggcatttg gatataatac gattggtata cctgtagcag caggaatatt atatgtattt
     6901 ggaggacctt tattaaaccc aatgattgca gcagcagcaa tgagcttaag ttcagtttca
     6961 gtcgtatcaa atgcacttag acttaaaaac ttcaaggcat ataagagaga ttagattaat
     7021 aaattaaata aaatgtatcc aaaagagcta tctttaaatt aaaaggatta atttacaaat
     7081 taagatagct cttttttcat gtaaaattca caacaataag ataaaatata agtaatatac
     7141 gaaattttaa ataaaataga aaggattgat agaatgaatt cgtcaatact tgtaattgaa
     7201 gatgattcaa atatacaaga gttaatatca gaatttttaa gtgcagaagg ttatcaagtt
     7261 gatacagcaa atgatgggtt ggaaggtata caaaagttta agcaaggaag ttatgattta
     7321 gtaatattag atataatgat gccaaactta gatggttatg gagtttgtaa aatgataaga
     7381 aagtcatcaa gcgttccaat tatattttta actgctttaa atgatgaagg agaccaactt
     7441 aaagggtttg acttagaatg tgatgattat ataacaaaac cattttcatt taatcttctt
     7501 ataaaaagag tagaagcaat tttaaggcga agtaacaaga ctataaatga caaatttata
     7561 gtttttgaaa aattaaaatt agatttaaac acatatattg cagagataga tggagaacct
     7621 atagaactta cactaaaaga gtttaatata ttaaaagctt tgatagaaaa gtatccacaa
     7681 gttataacta gggaaggtct tttggatagt atatggggtt atgactatta tggagatact
     7741 aggattgttg atgcacatat aaaaaatata agaaaaaaaa tatcgttacc atatattaaa
     7801 actgtaaaag gaataggata tacacttgag aaggatattt gataagtggg aaaagttaag
     7861 tataaagtat aagttatttt caataacgac atcattatta atagcgttag ctttaataat
     7921 atatctaatt ttatattttt tattgccatc atattatcat gagtataaaa ttgagtctct
     7981 tcaagaaagt ttaaaatctt tagtagatag ttcaattcat ttcgatacat atactttaga
     8041 agaaagactt tattatatgg ctaaagacca aaatttggct attttattaa aagataacca
     8101 agggaaaatt gtatatggta agaatgaagt agttatttta agatatagta agtacatgat
     8161 taatagtctt gaggatgagt atagaacttc tatacctata tatactaaag atgcaaaaga
     8221 tggtccatat actttagaac tcgttatgcc attacaacca attgacgaag caaacgaagt
     8281 tataagaaaa ctaatgccat acatcatatc aatagcaata ttgatagcga ttattggagc
     8341 atatatatat tcaattgtaa taacaaagcc tcttataaat ataatagaga gtgagagaga
     8401 acaggaatat agaaggaagg attttgttgc tacaatatca catgaactaa aaactccaat
     8461 aaccataata agtggtcaaa ttgaaggtat gatttatagt gtaggtaagt ataaggatag
     8521 ggatacttat ctgaaaaaat catatgaatg tacacaagaa ttaaaagatt tagtaaatga
     8581 aatgatagag gtttcaaaat cagaaatact tgaaaaagat ttgaaattgg tatcaataaa
     8641 tataagtgaa ttattaaata gacttgtaaa gagacaagta tttttaatag aagaaaaaca
     8701 tatgaaaact attttaaaaa tagaagaaaa tttggaaatt aaagcagacc aagagagaat
     8761 aactaaggca atcaataata taataaataa tgccataaaa tattctcctg aaaaatcaga
     8821 gcttataatt agactctatg ataaaaataa acgaataagt aaaaagactt ctaatcaaag
     8881 agtggtttta gaaattgaaa atactggtgt aactatcgag aaacgttatt tagaagagat
     8941 atttaatcca ttttatagaa tagaaaaatc ccgttcaaga aaaactggag gaagtggact
     9001 tggactttat atagtcagtc aaatttttaa aagtcatggg tttgattata gcataaaaaa
     9061 caaagaaaac tctgtagtat tcacagtaga atttaaaaat taattactta ttataaagaa
     9121 tatataactt tcaataatag ttcattttaa aatcacaaaa aattcataat gaaactgtat
     9181 tatataaata taaagaagct taaaaccata aaatacacca actataatga tgagatttcc
     9241 ttacatcctc catataagtc atcattatag taacccaaca attaagttac tatgaattta
     9301 aaaaataaca ccgagcatat aatctatgct tagtgttatt tttattttga aataaaaaat
     9361 atttaattta ttgaaatgta tagatttaga cagtttaaac tatatttaag acttaaaaat
     9421 tataattgat attacaaata aatatatgta ctttgggaaa aataaatata ggaagtatta
     9481 tatttacaga ggaggtagtt ttgatggata gattaaaaag agatatgaaa aatatgaaag
     9541 aagatttaaa agaaggtgtt aaaaagacag cttatgttat gaaaaataca gctgaagatt
     9601 tgaaagatga tgcacaaggt gctatggtaa acttagaaat gaaaaaagat gagatgatgg
     9661 aaagatatga acaaaagaaa ttttctaaag aattagagaa gcaaatgaag aatgaaatga
     9721 aataagcttt tatttatgaa taacaaatta ttgtattcat ataaaaagta gccagtaata
     9781 ttaaatagtt tatttaagga aatagacttt ctaaaaataa ctgtattaga tttagaaagt
     9841 ctatttcaaa ttagttttat aaatagacat aaggataaag tagatgttat agaactagat
     9901 tttaaaaaat agatactaag gataaaataa atattataga aatatgtttt taagaatagt
     9961 tatcaaataa aaatttatat ttataagaaa tcctataaaa tatataatta aacttataaa
    10021 acatttacat taaattctga taaaatatct actatatcag gagtaggcgc tttttctgta
    10081 attatagaac taatatcttc taaatgagca aacttgaaat ttccatcaaa tttaaacttt
    10141 ttatcttcca taactataaa tactttttta cttgaattta ttatagcctt cttcgtgaga
    10201 ccatcttcaa tttcaaaagt tgtaagtgaa gagttgaata catctactcc acaacttccc
    10261 ataaaagtct tatcaaactt atatttcgat ataaactcta tagttggagt accgacaaaa
    10321 ccgtctaaac tgagatttaa taaacctcca gtggaaatta tatttaaatt atttggtttt
    10381 gaagtaacta catttagtat atccaacata tttgtaacaa gtgttacttt tttattactt
    10441 tcagctaaca actttgcaag caagatgtta attgtagaga tatctaaaaa tatagtttct
    10501 ctatcttcaa ttaaattaaa tgctttttct gctattattt tctttgttgg aatattaatt
    10561 tcttttctgt cttttgtatc ctgattctct agtgtttctc tagctagtac agctccccca
    10621 taaattcttc ttattgaaga aatcttttct atatttttta aatcttttct tatacaatct
    10681 tctgtaacat taaatttaga actgagctct ttgacagtaa ctctaccttc tttttttagg
    10741 atatttaata tagcttggat tctttcttct gcaaacattt tctacctctt ttcgcaatat
    10801 agtattatag taaatattat aagataaaat acaatatatc aactatttta acatgaaata
    10861 caaaattaat ttaatat
//
LOCUS       DAEPOG010000128        10861 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEPOG010000128 DAEPOG010000000
VERSION     DAEPOG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.128"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(211..1302)
                     /locus_tag="KR432_002720"
     CDS             complement(211..1302)
                     /locus_tag="KR432_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641241.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1532..2617)
                     /locus_tag="KR432_002721"
     CDS             complement(1532..2617)
                     /locus_tag="KR432_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0641242.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2653..3966)
                     /locus_tag="KR432_002722"
     CDS             complement(2653..3966)
                     /locus_tag="KR432_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0641243.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3967..6258)
                     /locus_tag="KR432_002723"
     CDS             complement(3967..6258)
                     /locus_tag="KR432_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0641244.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6248..7042)
                     /locus_tag="KR432_002724"
     CDS             complement(6248..7042)
                     /locus_tag="KR432_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0641245.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7081..7527)
                     /locus_tag="KR432_002725"
     CDS             complement(7081..7527)
                     /locus_tag="KR432_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0641246.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7599..9005)
                     /locus_tag="KR432_002726"
     CDS             complement(7599..9005)
                     /locus_tag="KR432_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641247.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9023..10393)
                     /gene="hydA"
                     /locus_tag="KR432_002727"
     CDS             complement(9023..10393)
                     /gene="hydA"
                     /locus_tag="KR432_002727"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0641248.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10526..>10861)
                     /locus_tag="KR432_002728"
     CDS             complement(10526..>10861)
                     /locus_tag="KR432_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0641249.1"
                     /translation="KVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKE
                     AGIDSEITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYY
                     GILKSVYGK"
BASE COUNT         3150 a         2208 c         1129 g         4374 t
ORIGIN      
        1 ttttattcga ttttatcatc taaattttag gttttatttt tataaattaa ttgtatttac
       61 ataaaaaaaa agacctaaaa acaaattttt aacggtcttt tctcaatttt ttattttcat
      121 ttctaaaatt tataataaat agtaagaaca ctataaattc ttttatataa aattcttagt
      181 ttgtggatga taaattagtg aattaaaata ctactttact gattttagtt tgacaatatc
      241 actccaattt ttactagtgg ctaactctac taaatttaag tctcttctta tttcagctat
      301 atcctctttt atagaattag tgtcaccttt tatatatgct atgttatgtt ctatattatc
      361 atgagtagct ttatttattt cagaattatg taataaactt cctaaaatat tattctgttc
      421 tatctgtatc tctttcattg atttatcttc acattctaga ctatcaatct taatttcaat
      481 tccatataat ttttcatttg ctatggatac ctcgtcctta attccattca tatcttcatt
      541 aatgttattt attttatttt gcataaagtt tacttctgat ttaacttcgg aaacatcttc
      601 tttcattaca gttatttctt gtcttacttc tgacatgtct tttttcatta catttatctc
      661 ttgttttacc tctgatgtat cttctttcat tacagtcatt tcttgtctta cttcagacat
      721 atcttctttc attatattta tctcttgtct tacttcggac atatcttctt ttaatacatt
      781 tatctcctgt tttacttccg acatatcttc tttcattaca gtcatttctt gtcttacttc
      841 ggatatatcc tctttcatta tatttatctc ttgtcttacc tctgacatat cttctttcat
      901 tacagtcatt tcctgtttta cttccgacat atcttctttc attacagtca tttcctgttt
      961 tacttccgac atatcttctt tcattacaat catttcctgt tttacttccg acatatcttc
     1021 ttttaataca tttatttcct gttttacttc cgacatatct tctttcatta cagtcatttc
     1081 ttgttttact tccgacatat ctccttttaa tacatttatc tcctgtttta cttccgacat
     1141 atcttctttc attacagtca tttcttgttt tacttctgat atatcttctt tcattacagt
     1201 catttcttgt tttacttccg acatatcatt ttttatgcta actacatctt catttatagt
     1261 atataataac tctaaaatct tctcttgcac ttgcatcacc atccctatta attattataa
     1321 cattttacac aaatgctata caatgttaac atatatatca gaattattaa aattttaatt
     1381 aaacaaaatt gattcacttt attacttcaa aactaattaa tatctaaaat aactacaaat
     1441 aaacaaaaat aaggtcaaca acaaaaaagg cttatctctt tgagatagcc tttcttattt
     1501 ttatttaaaa ctttgttttt tataaaataa tctatacatt cagctctgaa catgaaattt
     1561 ttcttccaag tgcttttggt ttacaaactg atatacaaag tccacaatat ctacactctt
     1621 cttcattcaa ttccatctta tcattcaatt ttctcgcttg atatgcacaa actctctcgc
     1681 acatcttaca tttagtacat ttttcttcat cataagtaaa ctcaaagcta agtctttcat
     1741 taacatctcc catatgatta tgtactatac cacaagcatt ttctatagta ttatatccta
     1801 atctattaag attagatttt aaatcatcat gtattttaga tattacttgt gcgcctttta
     1861 aaattactac tgaacatata ccaacaaagt cagcacctgc cataatcatt tctaaggcat
     1921 catctccact tacacatcca cctaagccaa ttattggtat attaacttgt ttttttattt
     1981 catatattct ctgtaaagtt attggcttaa tagtttctcc acttaaatat cctattccat
     2041 ttccacctaa taaaggttgc cctgtttcta tgtctattct aaatgctggt cctacagagt
     2101 cacaagcagt tattgcatct gctcctgctt cttctaattt tttagcaaaa tcgcctatct
     2161 catctatcat aggaggaagt ttaactatta caggtatatt tactctttcc tttgcatctt
     2221 taagcattgg tatcaaatct ctatagtcat aagatactaa ttcaataaag tcagctcctg
     2281 catttgcaac tttctctact aattcgctac tttcttcaat tgtatgacct actgaagcta
     2341 tacagacagt cccatctctt ttcatttgtg gtatttcaaa atctatccat tgctctggct
     2401 caaaatctgt ccatttttca ttatttaaaa gagcattagc tgtatttcta accatatgtg
     2461 gtgctggatt tatagctctt tcttttctta tagtcttagt cacaactgcc cctgctcctg
     2521 catcacttaa tatcttgcat ccagctgcac tatatgttaa tggtcctgaa cctattataa
     2581 gtggtgattt taattcttta cctaaaaaat tatacattat ctgcttcccc ttttcatata
     2641 tatttacatt cgttaaaatt tccaaaattc acaagcaact tctttcattt ctttaacaat
     2701 agctttttta tctaaatcaa ctaactgttt ttcttttaaa atatgtctac catctatgta
     2761 tacatttgat ataaattctt ttttaccctg aactactatc tggtcaaata tattatcaag
     2821 agtcacagga gttttaaatt catcttccat aactattata tcagccttat taccaactgc
     2881 tattttacca gaattttgaa gtcctaatac ataagcacca tgctctgttg ccatcctaaa
     2941 tactaaattt gctggcatta ctgatgcatc ttcttcaact gatttatgca ttaaaaatgc
     3001 acctctcata acttcaaaaa aatcatttat atatccatca gtacccagtg ccacttttat
     3061 gcctttttta atcatttttg gtactggaga aaaacctcca cctacttcac agttgctaag
     3121 aggcatatga accactttta cacctttttc ttttaatata tctatttctt catcatttac
     3181 ttttacacac tgagatgcta atacagtttc atccaatata tctaaatcat tatagtagtc
     3241 aactgctttt ttgccaaaat gtttttcagc atagtttggc tcatatatac tctcacataa
     3301 atgaaattgc attggtgcat ttaatttttt tgcatcttct ttagctttct taataaatct
     3361 atcagaacat gtgaagcttg tatgtgtaca cattattcca tttattaatt tactattttc
     3421 tctactccac tttattaaat tagaattttc atctaaacat ctaagtccat tttcaaagct
     3481 tattctttca caactttcta aggataaaat cgctttcatg cctatttctt ctagtatttt
     3541 tccttgctca attaaagttc cttcttctgt atttggagct tctagggtat cacaaaaagc
     3601 taccacacca gattctataa gttctattgc tgatgcttta gttgttgcct tcacttcttt
     3661 caatccaatt ctattttcta taaatggcca ccaatagtca tttaaaaaac cttcaaagtc
     3721 agtaaaatgt acatttgccg gtattcccct agaaagaact ccatattgat gcatatgacc
     3781 attgacgaat cctggcatta ttatagcatc tgatttatct attatttctt caatattttt
     3841 atattttatt tcaacttctt catttggcaa tatatctttt ataatgtcat tttctactac
     3901 agcagcagaa tttgtatata tttcatcagc agaagatatt aagtatttac ttttaatagc
     3961 aatcatttat ttacctccct gatttaattt tttcatggcc atataaactt tctcagaagt
     4021 tataggaagt tctcttatac gaactcctgt ggcattgtat attgcatttg ctattgctgg
     4081 tgcaggtgta tgaataacta tttctcctaa agatttagct ccatatggac ctgttggctc
     4141 ataattatct acaaaatcaa caattatgtt tcctatatct ttttttgttg gaactttgta
     4201 ttgtaataga ttagaagttt gaagctttcc atcagaacca tgtctagggt cttcatacat
     4261 tgcaagccct ataccttgag ttacaccacc ttcaacttgt attctagcaa gtgcaggatt
     4321 cataactgta ccacaatctg tagaacaagc ataatttatt actttaaact cccctgttgt
     4381 tttatctaac tctatttcag caaatcctgc taagaatggt ttagcactct cttgtatacc
     4441 ataagattca gatgaatata aaacttcttg atttccacca ccaacagatt cttttcctaa
     4501 ttcacttaac aaaacaaact tctctacatc ttcactataa caaactctgt cttcatatag
     4561 tattaaatca tctattggta aatcgagttt cttctgtgct acttttaata tcttttgttt
     4621 taaactttca gcagatttta ttgtagcatt tccagttaca taagtagtac aagaagcgta
     4681 tgcaccagta tcatatggac acatatctgt atctcctgta taaacggaaa ttctatcaat
     4741 agatgtattc aatgcttttg cagctatttg tgcaagtatt gtgtcagaac cagttcctaa
     4801 gtctgaagac ccagaaaaca acttataagt tccatcttca tccatcctca tatttactat
     4861 tgccatgtct atgttggcta taccagaacc atgagttccc acagacattc caacagccct
     4921 caccttattg tttccaatat ccctaacagg gtacttttca tcccatccaa taagtttttt
     4981 acctctaatt atacattcat caagtgcaca acttcttatt ggaaaattca ttattccgcc
     5041 ttcagtttgt ttttttataa tattctttaa ctttatttct gttgggtcca tattaagttt
     5101 atgcgctaat tcatcaattg cacaatcaag tgcaaatgta ccttgtgttg ctccatatcc
     5161 tcttaatgct cctcctggaa ctaaattagt atatacagtt ctcccatcaa attttattgc
     5221 aggtacatta ttatatgttg gaagtacatt atgacctgat tcagagcaaa ctgaaggtcc
     5281 attatcacca taagcgcctg tattattcaa tgctttcata tctatagctt ttatgtttcc
     5341 ttctttatct gccccaattt ttatatcaaa aaacatctga tgtctagtat tagtcattgc
     5401 aaaagtttct tctctagtat aaattagttt tgctggtctt tttgtcatcc aagttacaaa
     5461 tgatgcatat atctctgtta cagctatgtt ttttccacca aaacctccac ctattcttgg
     5521 ttttattact ctaacttttg aaagaggtat tcccagtgct ttagaaacct gtcttctcat
     5581 atgataagct gattggtttg cagaagttac tactaatctg ccattcgtat ctatatacgt
     5641 ataagctcta tgagtttcca tcatacaatg ggcttgtgat tgagtcatat aagaatgttc
     5701 tactattaca tcactttctt taaactcttt ttctacatcc cctttaccca ttttttctct
     5761 cgatacaata ttttttgtag catccaatcc aaaatcaaat ggacaaaaca aatcatcttc
     5821 ttcatggatt aaaattttat ttccttccga ttctcttgca tccaatatag gagtcattac
     5881 ttcatattca acttttataa gtttcatagc tttaatagca gccctttcat cttcagcggc
     5941 tattatagca acttcatctc ctacatatct aactacgtct tcaagtatta attggtcatg
     6001 aggtgatgct tctggatatg actctccaac cattgaatat ctataatttg gaacatcttt
     6061 gtaagtgaat atgtttacta caccttttac ctttaatgca ttttttgtat ctatgttttt
     6121 tatcttcgca aaagcatgcg gacttctaag aagcttaatt gttaaaacat ctttatgaaa
     6181 aattaaatct tctgtatata ttggttttcc agtaacaatg gcttcactat ctatttttct
     6241 tacctcttta tttaccatca cactcacctc caatactatt atacattctc actactaagg
     6301 cttttgccat atcttttctg tattctttac ttcctcttat attacttcca aatgaaagtt
     6361 cttcacttga tacttcacca gctttttcta tatcattttc taaacttaat atttcacttg
     6421 ctttataggc aatttttgct cttcttggtc tagcgcctat cgcaatttta taaatacttc
     6481 cttcttttaa cattgctcca tttaatactg caaaatcacc tgtacatttt cttatagaat
     6541 caaatacacc tatagcatct tttttaggta atataacttc tattaatata tctttttcaa
     6601 gctcactttc taaaaaatca gctaagttca taaccccttt tttatataac ttaacctttg
     6661 catctaccac tagtaatgaa ggtattaaat ctgaaaatcc atacttagca aatacgctag
     6721 ctcctactct ggctccactt ctaaattgaa ctcctactat attagaaaca gcgtttgaaa
     6781 ttaccccatt acagtaattt ttaataacct tatttaattc taaagttctt aaacttgtgt
     6841 ctgctccaat taatatgcta tcatcagttt catttatata atctaaagct aagtctttta
     6901 aatctattgc tgagccaata tttttactac ctagttttaa aaatccacat cctccaagta
     6961 ttatgttgtt ttttcttgat attagcagtt cataagcttc atctaaactt tttggaacta
     7021 catattcctt tatagttatc atactctctc cccatatcaa cttaattttc acttaaaagt
     7081 ctaagtgttt aaatatttat gtattgctct taattgacct ttatatcctg tacatctaca
     7141 aaggttacca tttacataat ctataacttc ttcttctgtt ggattttgaa gctctctttt
     7201 cattgccaaa accatcatta taaatccagg agcacaaaat ccacactggt cagccccttc
     7261 gtctgcaaga aaatctccaa acttctttgc ttcatccaag accccctcaa gtgttgtaat
     7321 atgctgacca tctactctag cagtaagata attacatgaa agcacggcct tgtcatttac
     7381 catcactgta cataacccac atgagcctgt atcacaccct tttttaacac ttacatatcc
     7441 attttctctt aatgtatctg ctaaaaaatc atctggtctt atacttaatt ctttttcttt
     7501 attatttatg gtacatttga taatcaaata aatcacctca aaactcatat ttttaagcta
     7561 aagttttact atacttttct atgaataaat tacatttatt aataaaggct attccattta
     7621 tgaccttcca ttctattttt tatagttcct cttacacaaa gtctctcagc tgtatcttga
     7681 gcatctttta atgctttctc ttcattagct gtcaatattt ttgaatcttc cattattata
     7741 tttccatcta ctataacact ctctatattt ttcatactag aagaatatac taatgtagat
     7801 accggattat gcataggtat cgcttttggt gaaagcattg gattaaatat tagtaagtct
     7861 gcttttttac ctatctccaa tgaacctatc tcatcttcca ttccaatagc tcttgctcca
     7921 tctatagttg ccaactcaag aactttttct gccgacattg ctaatgggtc acacttatta
     7981 actttatgtt gaagtgctgt taatttcata agttcaagca tatcttgaga gttattacta
     8041 gcggctccat ctactcctaa actaactgtt attccttttt taagcatttc tggcactggt
     8101 gcaacacctg aagataagta catattactt gctgtattat gagacacttt catatcgtat
     8161 tttttagtaa gttccatatc tttttcagtt agatacacac aatgtaccat aagcacattt
     8221 ggtccaagta ttcctaattt ttctaaaaca tctatgtcat attgcccatg caattctttt
     8281 gctgcttctc tatcaaaagg agtctctgat atatgtactg taaataaagc atcatcatat
     8341 tcttttacca ctctccatag catttcaagc atttcttgag aatttgacca tattgctgca
     8401 ggagctactc ctattttgat tcttccattt tctgtattat ggtgtttttc aaacaatctt
     8461 cttacatctt tttcaaccgt ttctacatct tgcattattc caggatgaac tccaaattga
     8521 gctcctgtat tcatacatcc tcttccaagt attcctctta ttcctaattc tttataagca
     8581 tctattattc catcacaaag acctggctta ctatgaggat acatgtagtc aaccatggtc
     8641 gttataccac ttctaagtcc ttcaatacat ccaagcattg cagcatcata agtatctttt
     8701 ggctctaaat agttagctgc tggaaatgtc atagtttcta accagtcttt aagtaccata
     8761 tcatctccca atccttttaa taaagtttgg aataaatgat tatgtgtatt tataaaacct
     8821 gggaataaaa ctttaccttt agcatctata atttttttaa catcagtata tttactttct
     8881 atctctttag aatttccaat atcagcaatc ttgttatctt ttactactaa tgccccatca
     8941 aaaataactt ctctctcttt attcacagtt actattatgg cattcttaat aagtatatcc
     9001 atcattctta tttccccccg cattatttag taatttttct tttaatatat tgacctgttg
     9061 gatgttcttt aacattacaa ctttgtacta catgatttcc tctcaaaaat acatcttcaa
     9121 ctttacattt aaccttaaat ccttcatatg gagtataatc tacattttgt ttttgagttt
     9181 tataactaat tgtttcttct ttattgatat ttaaaatgac tatatctgca tcacttccta
     9241 cagctatctc acctttttta ggatacattc caaatatttt tgcagcattc gtagaagtaa
     9301 cttccacaaa cttatttaca cttattctat tttctaagac cccatatgta tataaaagtg
     9361 ctaacctatg ttcaacacca ggtgctccat ttggtatctt actaaaatca tcaattccca
     9421 agtctttttg ccccttaaaa ttaaatgaac agtggtctgt gccaactgtt tgaatatctc
     9481 cactagacaa tccttcccaa aggtaattta tgtcttcttt ttttcttaaa ggaggagaca
     9541 ttacatactt agcaccctca aatttattct tattatataa attgtcttct aataatagat
     9601 actgtgggca tgtttctact atcatatcta tatttttttc tctagccatt tttacagttt
     9661 caagtgactc cctacaactt aagtggacta cataggattt tgaattagat aatttagtta
     9721 tatcagcaag tcttgaaaca gactctttct caactatgtt aggtcttgta agtggatgat
     9781 attttggctc taaattacca ttttgtatat tttcttctat caatacatct agtaaatccc
     9841 cattttcaca atgaaatcca actatacaac ctaaatccct tgctttcttt aaaaccctgt
     9901 atattgttcc atcatctact ttcatgccat catatgccat atacatctta aaagatacta
     9961 tcccatccga aataagtttt tccatatgat taaatgtatc tttatttaaa catgtaattg
    10021 tcatatgaaa accataatca caataacaat ttccacttgc ttttttatga tatgcttcta
    10081 caccttgcag taggttttcc ccctcattgg cttctgcaaa atctagtatt gtagtagttc
    10141 ctccagctat agctgctctt gttcctgttt caaaattatc tgcagttgta atagaacctg
    10201 cattcatatc aaaatgagta tgtgtgtcta ttccaccagg aattacaatt ttacctgtag
    10261 catcaataac cttatcacca tttatttcta agttatttcc tatttcaact atcttttcat
    10321 cttctatcct taaatcactt atataagtct tatcagaggt aattatagtt ccacctctta
    10381 gtattgttcc catatatacc tcctaacttt taagctttaa agtattttct aagtatcgta
    10441 aaactttatt taaaattgta ttttctaaag gtgctatatt cagcacctct agactatatt
    10501 taaataattt attgtgaact aaatattatt ttccgtatac agattttaat attccatagt
    10561 aaccttctgt tcctataaat aactgttctt gctctatata ctcatctatt gtgtgagcaa
    10621 gattttcctt agaaggacca aatcctatag tttttattcc agcttcacct gcatagtgac
    10681 ttccattcgt acagaatgag tattgagtta tttctgaatc tattccagct tcttttaatc
    10741 ccttatatgc tgcttgaaca aattcatctt cttcgtcata taaccaacct gggaagaatc
    10801 tttcactttc tatagtattt cctgtatagc aatcagcttt ttcaaccgca taagaaactt
    10861 t
//
LOCUS       DAEPOG010000129        10807 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEPOG010000129 DAEPOG010000000
VERSION     DAEPOG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.129"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            72..545
                     /locus_tag="KR432_002729"
     CDS             72..545
                     /locus_tag="KR432_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641250.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(914..1471)
                     /locus_tag="KR432_002730"
     CDS             complement(914..1471)
                     /locus_tag="KR432_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641251.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1729..2427
                     /locus_tag="KR432_002731"
     CDS             1729..2427
                     /locus_tag="KR432_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0641252.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
     gene            3449..3916
                     /locus_tag="KR432_002732"
     CDS             3449..3916
                     /locus_tag="KR432_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0641253.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            3927..4142
                     /locus_tag="KR432_002733"
     CDS             3927..4142
                     /locus_tag="KR432_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641254.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            4582..4755
                     /locus_tag="KR432_002734"
     CDS             4582..4755
                     /locus_tag="KR432_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBH0641255.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(4912..5196)
                     /locus_tag="KR432_002735"
     CDS             complement(4912..5196)
                     /locus_tag="KR432_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641256.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     repeat_region   5307..5990
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5433..5460
                     /rpt_unit_seq="tttacattccatatagttaatataaaac"
     gene            complement(6821..7693)
                     /locus_tag="KR432_002736"
     CDS             complement(6821..7693)
                     /locus_tag="KR432_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0641257.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(8087..8665)
                     /locus_tag="KR432_002737"
     CDS             complement(8087..8665)
                     /locus_tag="KR432_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0641258.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(8723..9439)
                     /locus_tag="KR432_002738"
     CDS             complement(8723..9439)
                     /locus_tag="KR432_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH0641259.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9630..10715
                     /locus_tag="KR432_002739"
     CDS             9630..10715
                     /locus_tag="KR432_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0641260.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
BASE COUNT         3790 a         1520 c         1115 g         4382 t
ORIGIN      
        1 tattgaatta aatttattta agatatataa ttataattat aattaaaata ataataaagg
       61 tgttgtttat aatgttcaaa gaatacttaa taagagaatt aaaagaagat gatattaaaa
      121 atgcattaga cttaatctgg aatgtatttg aagaatttga atctccagat tattgtgatg
      181 aaggaattac gacatttcat caatttatag aatataattc tattattgaa aaaatcaaag
      241 aaaaaacact cttcttttgg ggttgttttg actctaaaaa acttattgga gtcttagcta
      301 ttagaaattt aaatcatata tgtatgttat ttgtagataa aaactatcat aaacatggtg
      361 ttgcaaaaaa attaatgact acaggcatac aatactgtat ttgtaataaa tttaatttaa
      421 ctgaaataac tgtaaattca tcaccttatg ctgttgaagt ttatcataaa ttaggatttg
      481 aagatattgc tgttgagcaa attgttgacg gtatacgttt tacacctatg aaattgttaa
      541 tataataaat taaataaaaa gcaaagtctc ttaaatattt tatagttact ttgcttttaa
      601 accaagattt aaaataatac tatctttttt gttttttaat aatatacttt aaaagcctta
      661 ctaatatgaa ctataattta ttttttgata tcctttttga ttaacatata aattaattca
      721 attattaaac ataaactatg ttgtttctct ttatatctct tctttgtaaa caatattggg
      781 catttgaaaa taaatattct taaaatatac tgttctcctg ttgattttcc tttggtacaa
      841 atgataaaat tttaatttac atgctccgta atgaagtgtt taataaataa gttaaaataa
      901 tttatttacc tccttaatca tattttttat gaatacctaa taacacagta tcaatagaat
      961 cggctaaacc ttttatagct tcttctttac tcaaaagtga tttcccaagt tgcaaagtcg
     1021 aataaccatg aagtacactc attattaagt ttaatacttc acctgtattc tttgtattta
     1081 gattacaaga aagaataagt ttttctaata aggatttata ttcatcaaag atttctaatg
     1141 tttcattatt tccatgccaa gtagcccact gaattgtttc ataaacacca ggattagaaa
     1201 ttataaaatt aaagtatgct atacttatac actttatagc tgtatctcca aaatttccaa
     1261 tagtcgcctt tatcatttga ctattcatct ctctcattcc tttatgagct acttcaagta
     1321 ataaatcatc tagactttct atatgattgt acaaagatgg tgtacgtatt cctagctttt
     1381 ctgcaataac ttttaaagaa actttgttaa gcccatcttt attaactata tctgatgtag
     1441 cttgtataac tatttctcta ctaacacgca tgattttccc tctttcattt aaaattaatt
     1501 actttattag tttagctaat ctaattagtt tcgtcaatta ttgtaattta taaaattctt
     1561 tatactctta taaaatcttg acagtttaat tataatttaa tataatacta attgaattag
     1621 ttttaatact aatataatta gtattatatt aaattatata ttacaacatt attttatagt
     1681 ttgattaatt caacaatcac taatatatca aactgaaagg agatttttat ggaaaaaaaa
     1741 tatttcgaaa ttcatggagt tccttcaatc ctttggggaa atccttcaag taaattatat
     1801 atttacatac atggacaatg cggcaataaa gaggaggctg aagcttttgc taatattgca
     1861 gttccacatg agtatcaagt tttaagtgtt gatttgcctg aacatggaga acgtaaaaga
     1921 gaaaaaaatt catttaaccc atggcatgtt gttccagaat tagtatctat tatggaatat
     1981 gcaaagtgcc actggaatca aatttcgtta tatgcaaata gcattggagc atggtttagt
     2041 atgttaagct ttgaaaaaga gaacttaaga gagtgtattt ttgtatctcc aattctagat
     2101 atgcaacagc tcatttcaaa tatgatgctt tgggcaaatg tatcagaaga acagcttaaa
     2161 catgaactaa ttatcccaac ttcatttggg catactcttt catgggaata tctacaatat
     2221 gtaaaacaac atcctatcac tcaatggaat gtaccaacaa aaatattata tggtaaacat
     2281 gatgaactta ctgaaatcgg tgttgtagag aaatttgtag aaaagtttaa ttgcaacctt
     2341 actattctgg aaaatggcaa acattggttt aacacaccac aagaattaaa ttcattatat
     2401 gaatggttaa atactttagt taaataaaaa ataattatat tattaatatg attaaattat
     2461 ttataaatgt tatttaatta attttatttt agcattatta aaataactac ataaagaata
     2521 aattagttat tattaaagaa attaaaagta tatagagtat taataatttt ttaataatta
     2581 gttattatag aaaaaagagg gatataatat tgtctccctc tctttcttct gtaatattat
     2641 ataattttta tttatcactc tcaataaatt atagttatct acaaaattca atctttaata
     2701 cattattaat atattgtgat attaatatta cagtattaaa taaaatctaa taaaattaac
     2761 tataaaatat ctattatata aatatccact cttatgtttg aaatctgtca tatagtttta
     2821 tccttcttat cttttatatc aataactttt tttaataaaa ttgacaaaac aaagtaaaaa
     2881 accttattga taatgaaaca aatgatacta taagttctga tattatagta attgcatata
     2941 aatagacatc ataacaataa tataatattg tcactataaa attaaatgta aaaatctcca
     3001 atctaactaa atacacatat aggtattttt taagttttta ggtatttctt ctgaaaatga
     3061 tttattgatt gataccaatt tacataatct ttttaatata aacaatgata caatatatgg
     3121 gacagcacat atgtatatag ctacttatat tacatattac agttggcata ttcgtatttg
     3181 taatgcttat accaatttta aaatacacca tcagtactaa tggcattaaa ataatctata
     3241 ataaagttaa aattattcca attagtacaa tattatttaa tattttacaa ctcatattct
     3301 tttttatata aatttaccac cttatacatt cttattttta gtgtatatta taatggtttt
     3361 atatattata tcaacaattt attattgaaa ttcaataaat tatatattat aatcaataat
     3421 atactttata taaaggagat gatattttat gattacaaaa ttttttagta atatattatc
     3481 agtatttcta tattttctat tatcactttt aggagttgct ggtataatat tcttatgtat
     3541 gatacctatg tttttatatg attttagttc tgacaaagta atcgctttaa ttgcaagaat
     3601 actatatttt tctgactact ttatagtcat cttattcttg atatttataa ttaatagtac
     3661 taagtattcc ccattcatac ttgaaaatgt aaaaaggttt aaagtaatgg gatgttgttt
     3721 attgataaac actataattg aatgtgtaat tggttataac tcaaatacta gtcttataat
     3781 gatatttgga gataatagtg gcggtatctc accacttatg gttatttctt ttatatttgc
     3841 actaatgtgc tttgtaattg cagaaacatt tgataaagct ataaaaataa aagaagataa
     3901 tgatttaacg atatagaggt gatgttatgg caatagtagt taacctagat gtaatgatgg
     3961 caaaaaggaa aatatcactt aaagatttgg cagaaaaaat agatattaca aatgcaaatt
     4021 tatcaatttt aaaaactgga aaagcaaaag ctattagatt taccactcta aacgaaattt
     4081 gcaaagcttt agactgtcag ccaggtgata tacttgaata tgtagaagat gaaaatgatt
     4141 aaaaaagagt gtttagaaac actctttttt attatactat atgattttta ttcttttact
     4201 atattattga cattaatatt aactttgttt aaagtcataa caaaatacgt tctaagaaag
     4261 aaactgttcc tttttctatt tgttatccat tacctataag aactgtatca cactaaatca
     4321 tacttttaaa ttgataattt tgccacagat tcctatatac attcttttct acctaatatt
     4381 tatttaaact gttagcaaaa catggaactt tttcatttac ttatacctcc atttatatta
     4441 ttgcatagtt tatagctacg tgttataaat tacattattg atgatttatc taacatttgt
     4501 aatcttttag atactctttg aagttacaac actgttatac tctaattaag taaactaaat
     4561 ctgatacaag ttggtgatta tatgaaaaga aaactttcaa cctattttaa aggatatatt
     4621 ttaggtttat gtatagcatt tttattattt acttttatat taaaaaaatt aattatagaa
     4681 aatatcattg gaataataat tggaactgga attgcatttt tactgattta cctatttaat
     4741 aaaaaaagaa aataatattc tataaaaagg tcttttagtg aatttacaaa aaattaataa
     4801 gaataatata tacttagtat ttaaaaaggg taacaactct ctctagctgt taccttatat
     4861 atttatctat atctttagta ttaagttttt taatttctac ttttttaatt atcaatttct
     4921 gtatgtagat atactaaacc ttccaacttg agatttaaat aacttttcaa tctcataaac
     4981 tattctatac accttatttt cgtactctaa atcataatac acatttattt cacctatact
     5041 gtttacactt atcgtataat gttctttttc tattatacat attcttccaa aatctgacag
     5101 gtcattatct atcaatttta actgtttttt tatttttgaa aaatctaatc ttggaaatat
     5161 gtcattagga taaaaacttt tattatatac aagcatttca tccacccctt atttattcta
     5221 tttatatcat tttataccct aattaataat ttattaatca tctttttcca tattattcaa
     5281 attctcttat ataaaaacaa aatcaattta gaatatgtat tattaaatat aaagtgactc
     5341 aatctttatt taataagaaa aaacatttat attacatata attaatataa aactaatgaa
     5401 gtgggtgtat gaaagagaaa acacaatttt aatttacatt ccatatagtt aatataaaac
     5461 agttgttttt attttttcta aagagaaaaa actagacttt acattccata tagttaatat
     5521 aaaactttct gtatatttta gttgtgtttt aatattataa cactttacat tccatatagt
     5581 taatataaaa catggcagta actaagaaga aaactatacc ttttttattc tttacattcc
     5641 atatagttaa tataaaacga ggaaatttta tactaatgac tgatgctaga tttaacttta
     5701 cattccatat agttaatata aaactattat aaggagcatt atacgaccaa aagccaaagc
     5761 agctttacat tccatatagt taatataaaa caagcaacag caagagtcgc gacaacatta
     5821 aaaacttcct ttacattcca tatagttaat ataaaacctt tatttttttc tctatatcga
     5881 gttcgccaaa ttcactcttt acattccata tagttaatat aaaacagtcc agcgttgtgt
     5941 ggtcgtaaca caccaatcct actttacatt ccatatagtt aatataaaac cccaaaataa
     6001 attaagtatt tccaatactt atacatatac aattctccta aacttgcagt gagcgagcaa
     6061 tagtgcaatt gataacagtt atcatacact ctcaacgctt tatatttcaa ctattaaacc
     6121 ctactttatc acaaaaatcg ctcactgcaa aacttttata tttttattat atcataaaac
     6181 tcatactata aaataaaaat gggataattt tacaaactaa aagcatctac attcttataa
     6241 gtatttatta tattctctat tccaatgatt cctttttctt tttattgcat ccttcctatg
     6301 taatcgcact ttagctcaac aagtctttta ttttcattaa caatttttta aactattcaa
     6361 aatcatcaaa tttttcatct tatacagtta aatcaaaata cgtgttatat ctctttatct
     6421 atatactttt cttccttcca tttctttttc tctttctaca ttttcacttt gttactttac
     6481 aatctcaagg ttattatctg ctattaaagt tttatctttc caggtttcac aaaaataaat
     6541 tgaataagtg caaaaataac agatacaaaa ccataactat tttcttttct ataaaaaaat
     6601 atatcatcaa caagaaacat aataaatgaa aataactatg aataaatact ctcatttaga
     6661 agaaatagac aaagatattc cttaaaattt aagcaaaata ttgtttaaat aaaaaaactg
     6721 actgtcagtt aaatgccagt cagctatttt atatatttta tccaaacatc actatatttg
     6781 aacattttaa taattaataa tacttattaa tatacaaact ttactcatac acagaatatc
     6841 ttttccaaat ttgctctaaa tcatcaaaat gctgtttaat atcttctttt tccttcattc
     6901 caacagatat agcaagggca tttgaaatac taagagctgg aactagagaa tctacaaatg
     6961 aagccatatt actcttaact agaagagtgt tatctgcaag tgatgctact ggcgcaaata
     7021 agctatctgt taatgatatt acatgggctc ctttttcttt agcataattg acaatttgat
     7081 aagatttctt agaataacga gggaaactaa tagcaactat tacatcttct tcatttattc
     7141 ttacaatctg ctcaaaagca tctcccatat ccattctaat tatatgaaca ttgtcaagta
     7201 ttatatctaa gtaaaaacct aaatattgag ctactacaaa agaactcctc attccaagta
     7261 tatatatttt tctcgctctt aaaagtttgt tagaagcttc tttaaacgct ctctcgtcaa
     7321 tttcttctag tgtagacctt atattatcta tatcactttt taaaactttg tttagtattg
     7381 caaaatcatc ggaataatca tgtgccattt caactctttg tactgtagtt agtttatttt
     7441 ttatgagttc ttgaagtgca gcttgtaatt taggatatcc actataacct aatgcatttg
     7501 caaatcttac aactgtagac tcacttactc ctacggtttc acccaattta catgcagtca
     7561 taaatgcaac tttatcataa ttatttaaaa tgtattgtgc tataagtttt tgacccttac
     7621 tcaatctcgt atattgagat tgtatatttg atatcaaatg tttactgtcc ttcatttcat
     7681 ttgtttcttc catttttttc tccttttagt tatataaact attttgtttt attaacactg
     7741 tctttttctc taaaaatttt attttttatc tcaatttcat cttagttttt atcatagctt
     7801 cttaaactca taaaattcta acttttctgt accttatact tcacatagta ttttcgatta
     7861 aatttattat accatctttc cttgcatttt ttaatattta tccaatattt cttttaaatt
     7921 tttcaatttt tactcattta aaagttatct atcttatttt gcatatttag atgtatattt
     7981 tgttttgaga ataagctgtt gtatacttat tatttaaagt atttttaata ggtcacacta
     8041 tatatattaa ctcttaatag aatcttgtca agttttttag tatttattat gcttttttct
     8101 tttcaattaa aatcaattgt ggtgggttat tgacttgatt taaaaactta gattctaaaa
     8161 cattaaattc atcttgattt aaattattga caaggttata tatgcaatct ttttcttctt
     8221 gacctccatc atgccctgta tatatggcaa tgcttattac tccattttct tttaacattt
     8281 ctaaacttaa ctttatagcc tttaaagttg tatctggttt ggttataatt gaatgtttcg
     8341 ctcttggaag ataacccaaa ttaaataaaa cacatgaaac ttcttctttt atatattgat
     8401 tcattttttc atgcccatca aatatcaact ttactctatc tgtatatcct tctttctcta
     8461 gtttctttct tgtagacttt attgcttctt cttgtatatc aaaagcatat acaaagccct
     8521 tttctccaac cttctcagct agataccttg tatcataccc atttcccata gtagcatcaa
     8581 ttactatgtc cccttcattt ataatatttt ctaaataaaa tttatttata tctgtaattt
     8641 tagttaaata ctttgcatat gccataatac tatttctccc tactattaac tttatttcat
     8701 tttttctcaa tttattttta tcttatttta ttgattttaa atattttatt tcatcttctg
     8761 ttaggtatct atattctcca actttggtgt cttttaatac tattttcccc acagaaatcc
     8821 ttcttaaatt taaaactgga tgatttatag ctttgcacat ttttcttact tgtctatttc
     8881 taccctcatg tatagttatt tcacacactg aataattttt ttctttattt tctttagtta
     8941 cccttatttt ggcaggtgcc gttgtgtaat cttctatttt tagtcctgtt tcgaattgtc
     9001 ttatctcatc atcacttata attcccttca cacttgccac atacgtttta tcaatttcat
     9061 gctttggatg ggtaagctta tatgttaaat cgccatcatt agtaagtatt aataaaccac
     9121 tagtttcata atctaatctc cctattggat atactctttc ccttacatct ttaagtatgt
     9181 ctattacact gggtctattg aattggtctt ttactgtagt tatatatccc tctggtttat
     9241 ttaatactat atatatataa ttttcacttg gttttacttt tttattatca aattctacta
     9301 tgtctttttc ttcatcaata ttaaaagata actcttttac tacacttcca tttaccttta
     9361 ttcttcctcc aagaattatt tcttctgatt ttcttcttga tgctattcca caagaagcta
     9421 tatacttatt aattctcata cttaatcctt ccttatctta aattatgtgt gtttaatatt
     9481 tttatcaatg ttaattttgc tacattatta tactatacta gaccttaatt aaaaacaagc
     9541 aaaaaaatta tttaattttc ctaaataatt gtaatttctt ttcacatact attaatatgt
     9601 aatagctgac taaacactat tttaaggaag tgattattat tttaaaggaa aatataaaat
     9661 attatttaat agtcgtgttt attggaattt tattttttaa atttattaat actccatcaa
     9721 actttatatc tagtattgaa ggattcttaa agttttttag cccattttta ttagctattt
     9781 tacttgcttt acttttaaat ccacttgtga tgctttttga ggtaaaattc aaagcacata
     9841 gacttttagc cattttttta tcttatatat ttataggttt tattttagcc tttgccatta
     9901 ggctattaat tccatcaata gctaatacat taaatagatt gataaatgaa atgcctatgt
     9961 atactgatta tatagatagt tttatagaaa aaaatatgtc aaacatagat tttctaaaaa
    10021 ctttaattcc tcatatacaa catagtttag ataatttatt aaaagaagca agtaattttg
    10081 ttggtaaaat tcctaaaaac ttcttaatct atacacttag tatatcttct atgctattta
    10141 atatgacaat gggatttata ctatccatat acattatata tgacaaagaa aaaattgcat
    10201 tgggatttaa gagatttcta tattcttcta ctgctagaaa caaggcagat aacattatag
    10261 aattttttag gactactcat gatattttct atgattattt acttggtaga atattagact
    10321 ctcttattat tggtataatt gcatttttag gatttcaatt tgttatcaga atagaaaatg
    10381 ctttattttt agcttcaata atattcttag gtaatataat tccctatttt ggtcctttta
    10441 ttggtgcaat ccctccaata gccatgacta tattatatag tccacaaaaa actatatggg
    10501 tcattgcatt tctatttata ttgcaacaat tagatggaaa ttttatagaa ccaaaagtta
    10561 tgggtaatca agttggtata ggagctatat gggtaatatc tgctattttg gtgggacaat
    10621 ctctatttgg tttcatagga gtttttcttt cagtaccaat agctgctgtt gtaaaaactt
    10681 atgttgataa atatatagat aatcgtttac aatagcttat ctctactgtg actttaattt
    10741 ttagtttaaa atttaactgt tatttattaa attatttctc aaaaaatatc tcttagtaag
    10801 aaaaaat
//
LOCUS       DAEPOG010000130        10712 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEPOG010000130 DAEPOG010000000
VERSION     DAEPOG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.130"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            311..1489
                     /locus_tag="KR432_002740"
     CDS             311..1489
                     /locus_tag="KR432_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0641261.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            1498..2154
                     /locus_tag="KR432_002741"
     CDS             1498..2154
                     /locus_tag="KR432_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBH0641262.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            2154..2819
                     /locus_tag="KR432_002742"
     CDS             2154..2819
                     /locus_tag="KR432_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBH0641263.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            2924..3700
                     /locus_tag="KR432_002743"
     CDS             2924..3700
                     /locus_tag="KR432_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0641264.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            3762..5096
                     /locus_tag="KR432_002744"
     CDS             3762..5096
                     /locus_tag="KR432_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0641265.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            5593..6519
                     /locus_tag="KR432_002745"
     CDS             5593..6519
                     /locus_tag="KR432_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBH0641266.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(6911..10450)
                     /gene="nifJ"
                     /locus_tag="KR432_002746"
     CDS             complement(6911..10450)
                     /gene="nifJ"
                     /locus_tag="KR432_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH0641267.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         3726 a         1730 c         1663 g         3593 t
ORIGIN      
        1 cttctaatga tattttatag gaaaatcaac ataatttcat taatatttta acaaaatatt
       61 tttctaaatc tactataaat ttttatccta taaatttttc ttatacacta tataaaaaat
      121 tagacttaga caaaatgctt ttttcgccgt aatctataat tagaaacagg aaaacctaaa
      181 gcattaggaa taaaagtaat tgaaatactt aaaaacaagc ttgtattaaa aaagtacttt
      241 attatagtgt tccctaattt ctttattcag aaaattttta attatcattc aagaaaggta
      301 actattagat atgaaagatg tagtaatcgt atcagcagtc agaacaccta taggaagttt
      361 tggaggtgta tttaaaaata cttcagctgt tcaacttgga actatcgctg ttaaagaagc
      421 gatatcacgt gtaggactta atttatctga aatagatgaa gtaataattg gaaatgttct
      481 tcaaacaggg cttggacaga atgttgctag gcaaattgct ataaattcag gtataccaaa
      541 ttctgttcca agctacacag taaataaatt atgtggttca gggcttaaaa gtgttcagct
      601 agctgcccaa tcaatcacat ctggtgaaaa tgatgtggta attgcaggtg gtactgaaaa
      661 tatgagccaa gctccttata tcgttccaac tgctcgcttt ggtagtaaaa tgggtgacat
      721 aaccatggta gatagtatgc taacagatgg ccttattgat gctttcaatc agtatcatat
      781 gggaataact gcagaaaata ttgcaactaa atttgaattt actagagaaa tgcaagataa
      841 attggcatta gaaagtcaaa ataaggctga gaatgctata aaaaacaacc gctttaaaga
      901 agaaatcgtt ccagtagatg ttttaatacg tcgaggaaaa atagaaacta ttgataaaga
      961 tgaatatcct aaattaggca tgacatttga aggtttagct aaacttaaac ctgcattcaa
     1021 aaaagatggt actgtaacag ctggaaatgc ctcaggtatt aatgatggtg cggctatgtt
     1081 aatactaatg agccaacaaa aagcagacga actaggtatt agaccacttg ctaaaataaa
     1141 atcctatgct tcagctggtg ttgaacctga agtcatgggt actggtccaa tccctgcaac
     1201 tagaaaagca ctgaaaaaag ctggacttag catcaatgat atagacttaa tagaagcaaa
     1261 cgaagcgttt gctgcccaag cattagctgt taaaaatgaa cttcaaatag attcatcaaa
     1321 gttaaatgtt aatggtggtg caatagcatt aggacatcca ataggagcaa gtggagctag
     1381 aatacttgtt accttaattt atgaaatgca aaaacgtaaa gttgaaactg gacttgcaac
     1441 actttgtatt ggtggtggtc aaggaatatc aatggtggtt tcacgatagg aggaaatatg
     1501 aataaaatag ttagcattga tgaagctcta tctcatgtaa aagatggaga tacaataatg
     1561 gttggtggat tcatggccaa tggttcacca gaaaatttaa ttgattattt atgtagtaaa
     1621 aatataaaag atttgacact aatttgcaat gatactggct ttattgacaa aggagttggc
     1681 aaaatggttg taaataagca atttaaaaag ataattgctt ctcatgtagg attaaataaa
     1741 gaaactggaa gacaaatgaa caacaatgaa acagatgttg agttagttcc tcagggaact
     1801 cttgctgaac aaatacgtgc agctggttat ggtctaggag gtatactcac accgactggt
     1861 attggtactc ttgttgagga gggaaagcaa aaaattgttg tcgatagcaa agaatacctt
     1921 cttgaaaaac caatttatgc agatgttgct cttctatttg catcaaaggt cgataaagca
     1981 ggtaatctag tttataaagg ctctatgaac aattttaata atttaatggc atctgctgcc
     2041 aaaattacaa tagttgaagc agatgaaatc gttgaaattg gtgacattga cccaaatgaa
     2101 gttaatacac caggtatttt tgtgaattac atcgttgaag gaggtaattt ataatggata
     2161 aattagaaat gcaagaatat attgcaaata gagtatctaa agaattaaaa gacggagccg
     2221 ttgtaaacct tggaataggt ctaccaacca aagtagcaaa ttatattccg gataatgtaa
     2281 acgttatctt acaatctgaa aatggctttt taggtttagg ttcagccgaa gatggaaatt
     2341 caagtgatga aactattgta aatgcaggtg gtcaaccagt tactatactt cctggaggat
     2401 gtttctttga tagtgctact tcttttggaa taatcagagg aggtcatgtt gatataacta
     2461 ttcttggcgc acttcaggtt gacaaatatg gcaatatagc aaactatatg attccaggta
     2521 agatggttcc aggtatgggt ggagctatgg acctagtaac tggagctaaa aaagttattg
     2581 tagcaatgga acatacttca aaaggttctg caaaaatatt aaataattgt actcttccac
     2641 ttactgcaac aaatgctgta gatttgatag ttactgaaat gggagttatg gaagtaacat
     2701 cagatggaat tttacttaaa gaaataaatc cagctttcac attagatgat gttatttctg
     2761 ctactgaagc tcctttaata ttgtcagatt cattaaatgg aaaaatatct gttgtttaaa
     2821 ataattaaaa ctagaaaata aaaatctctt agacgtatta catataaatt tgtattgctt
     2881 aaaatattaa tataaaaata aaataaaatt ggaggaaatg aaaatggtta aagataaagt
     2941 tgtattcgta acaggagctg caagtggaat aggtaaacaa atagctgaat cgttcttaaa
     3001 aaatggtgct aaagtaatgt tctctgatat aaatcaagaa gctttaaata ataccataac
     3061 agagttacaa aaaaaaggtt atgattgcat gagtgtaaag tgtgatgtaa ctaaggaaga
     3121 agaaattaac cacgctatag ataaaactgt tgaaaagtat ggcagattag atatattaat
     3181 aaacaatgct ggacttcaac atgtttctat gatagaagat ttcccaactg ataaatttga
     3241 atttatgata aaaataatgc tgacagcacc ttttatagct actaaaaaag catttcctat
     3301 aatgaaaaaa caaggctttg gtcgtataat aaatatggcc tcaatcaatg gtgtaatagg
     3361 atttgctgga aaggctgctt ataactcagc aaaacatgga cttattggac taacaaaagt
     3421 tgctgctctt gaagctgccg attctggtat aacagttaat gctttatgcc ctggatatgt
     3481 agatactcca cttgtcagag gtcagtttaa tgacctttca aaaactcgta atataccatt
     3541 agaaaatgtc ttagaggaag ttttgtaccc acttgtacca caaaaacgcc tattagatgt
     3601 tcaagaaatt gccgactatg ctatgttcct agcaagtgat aatgctaagg gtattacagg
     3661 acaagcttgc ttactagatg gaggatatac tgcacaatag ttattatatt tagggcttga
     3721 agaaagtatt cttcaggtcc ttctactaag gagggatatt tatgagtatt atcgcaccaa
     3781 ttggtataat tattggtatc atagctatta tatatttttc agttaaagaa attcatataa
     3841 ctatagctgc tccaatagca actttaatag ttgttctctt aaataaaatg gatgttgtaa
     3901 catcaatgct aggtgctgac aaaaacaatt atatgggtgc acttggaaac tatataatga
     3961 gttattttgc aatattctta ttaggctcaa tacttgctaa atttatggaa gaaagtggag
     4021 ccactgtatc aatagctgat tttattttaa ataaagttgg ctctaatcat ccttatagag
     4081 ttttagttgc tatatttata gtaagtttta tattaacgta tggtggtata agcttatttg
     4141 tagttatgtt tgcagtcatt ccacttgcaa gaactctatt taaaaaactt gatatttctt
     4201 ggaaccttat tcagatacca ctttggcttg gtattgcaac aattactatg actataattc
     4261 ctggaacacc agccattcaa aatgttatac ctattcaata tctaaatacg tctcttactg
     4321 cagctactat accaagtatt gtaggtagta taggttgtgc tacgtttgga cttgtctata
     4381 tgaaatatgc tctaaacaaa agtattagaa aaggtgaaac ttacgcaact tacacttcaa
     4441 aattagaaga agaatcttta gatagagaat taccaggatt ttttgcaagt atttttcctc
     4501 tattagcact cgtaattata gctataactg gaagtacttt tggttcagaa tttatgaaaa
     4561 aaaatgtaat ttacatagct ttaatagttg caattctttt atctgctttt ttattccata
     4621 aatttctatc atctaagata cagacattga gcataggtgc tagtggttct ataggtccta
     4681 tatttgctac atcttctgca gttgcatttg gagcagtaat aatggctgca cctggttttg
     4741 atgtatttag caaacttata atgtcaatac caggtagtcc acttataagt cttactgttt
     4801 tgacttcaac aatgtcagca ataacagggt cctcttctgg tgcattagga atagtaatgc
     4861 caaactttgc agattattat ttaagtactg gtctaaatcc agaattaatt caccgtgttg
     4921 ctacaatagc ctcaaatata ctaactatag ttccacaaag tggtgttttt cttacattct
     4981 taagcttatc taagttaaca cataaaactg gatttaaaga aactttcatt gtagttgcag
     5041 ttggttcact aatagcagaa attattgtaa ttacattagg attactaatg tattaatata
     5101 aaacaaatta aaattcataa tatgaaaggt ggtgtctcaa agtgaacttt ttacttaatg
     5161 agacactctt tttgtatgaa atcaaatata ttttaatcat ttcaacaata aaaaagaacc
     5221 tttctctatt ttgagacaaa ccctttttat tccttaaaga tttaaacaat tataatttgt
     5281 tactaatacg aaaacatcta ttgaataaat ttaaccaaat aaaaaaaaat ttatcataac
     5341 tataaattta cgttatggaa ttattataaa aatattattt tctctaagta agtaattcca
     5401 tactatacag gcttttaata caaattttca aacacactga aatataccta tttattccat
     5461 atatatcttt tttacatatc tttacagtaa aaaaagtctt aatcttatgt tagtatagat
     5521 tcacgggtac cccctacact aaaggttaca aatcagtaac atttttagaa tttttagatt
     5581 aagaggtgtt taatgatgag aagaaatata ccaacaaaat taaaaactat attagcttta
     5641 ggagtgttaa ctttagcaac agcttcaagt gctgttactg caagtgcttc atcacttgaa
     5701 aataatgtaa atataagtaa caaaagttta gtatatagta atactaaata caagttttct
     5761 gcaaaaccaa attgttctgt aaaaccagaa cttaaacctg ataaagataa ctcttgtaag
     5821 gataagaatc ataatgacaa gaattgcaac aacactaata aacctgagga caacaataat
     5881 tcagactcta ctaataagcc tgataacaat aaaccagagt ctaataaacc tgaggacaac
     5941 aataattcag gctctactaa taaacctgat aacaataaac cagagtctaa taaacctgaa
     6001 gacaacaata actcaggctc tactgataaa cctaataaca ataaaccaga gtctaataaa
     6061 cctgaagaca acaataattc agactctact aacaaacctg atgataataa caactcaggc
     6121 tctacaagtg aaaatttttc agcttaccaa aaagaagttg tcgacttagt aaatatagaa
     6181 agagctaaag ctggattaaa tcctcttact cttgattctt ctatatcaaa tgttgctaca
     6241 aaaaaatctc aagatatgat tgataacaac tatttttcac acaattctcc aacttatggt
     6301 tctccatttg acatgttgaa aaaatttgga ataagctata agacagcagg tgaaaatata
     6361 gctatgggtc aaaaaactcc taaagaagtt gtaaacgcat ggatgaattc tgaaggtcac
     6421 agaaaaaata taatgaatcc aaatttcagt aaaataggtg ttggagtagc acaaaaaagt
     6481 ggtggttcaa tttattggac acaaatcttt gtaggataaa atactctttt tccatgcata
     6541 tcaaaagagt atctctaagt gataaaaaac acaaatgaga tactcttttg tatctatttt
     6601 aaaattatca aatccaaata tacatactat aaatattatt tgttctctta attttagtgt
     6661 gtagaacaat atataaaaag tattggcaag atgaaaatgg aaaacttaaa aagatattta
     6721 ataggtaaaa acaggatctt aatatcttca taaaaaaagg ctgtctcaaa atagagataa
     6781 ggcctttttc attgttgaaa tgattaaaat atatttgatt tcatacaaaa agagtgcctc
     6841 atgaactaaa aagctcactt tgagacactc tcttttatca attctatata agcattataa
     6901 tactttaata ttattcagct aattttttgt atccttcata tctttcttta gcatcagctt
     6961 cagctttagc aaataatcca tcagcttcct ctgggaattg tttagctata gcagaatatc
     7021 ttacttgtgc aagtatgaaa tctttgaagc ttgcagttgg ttcttttgaa tctaaagtga
     7081 atgggttttt accttcagct tttacttctg gattgaatct gtataaatgc cagtatccgc
     7141 aagcaacagc ttgtgcttca ttagccacag ttcttcccat accttctttt aatccatggt
     7201 ttatacatgg agcataagct attattagag atggcccatc atgtttttca gcttctaaaa
     7261 cggctttgat aaattgattt ttatcagccc ccatagcaac ttgagctaca tatacgtttc
     7321 catatgtcat agccatcatt cctaaatctt tctttctaga ttttttacct gaagcagcaa
     7381 acttagccat agcagcagct ggagtagctt tagaagcttg acctcctgta tttgaatata
     7441 cttctgtatc aaatacaagt acgtttacat cttcaccaga tgcaagaaca tggtctaatc
     7501 caccgtaacc aatatcatat gcccaaccgt ctccacctag tatccattga gatctcttaa
     7561 ctagataatc ttttctatct tttatagctt ttactaattc tttagctttt tcatctgtac
     7621 aatcacaagc tggtttttct aataattcaa caaccttagc acttgctact ttagaagctt
     7681 ctccatcatt tctagaatct aaccactctg taaatactgc tttagcatct tcacagatat
     7741 ccatagaaac taactctgtc atagcatcta ctattttatt tcttatttgc ttaacagctg
     7801 tgtacatacc aaatccatat tcagcattat cctcgaataa agaatttgcc caagatggac
     7861 ctttaccttc atggttacaa gtataaggaa ttgatggtgc agaagctccc catatagaag
     7921 aacatccagt agcattagct atcatcattc tatctccata taattgtgta actactttaa
     7981 tgtacgcagt ttctccacat ccagcacatg ctcctgaaaa ctccattaat ggttgtctaa
     8041 attgacttcc tttaacagta tttgcaggca taacatcaac ttttggagct acttcatcag
     8101 gatttactgc aaatgcccaa ttatctaact ctgtatcaag ttgagtatct attggcttca
     8161 taactaaagc tttttcttta gctggacata tgtcagcaca gtttccacat cctgtacagt
     8221 caagtggact aacttgaatt ctatatttta atccttctaa tcctttacct aatgcttttt
     8281 tagcttcaaa tccttctgga gcattagcta attcttcttc agtaactaat actggtctta
     8341 tacatgcatg aggacatata aaagaacatt ggttacattg aatacagtta tctattatcc
     8401 attcaggaac atcaacagct ataccacgtt tttcataagc tgcagtacca caagggaatg
     8461 ttccatcttc tataccattg aatgtactta ctggtaagtt gtttccttct tgtgcagtca
     8521 ttggtcttag tatatttttt ataaattctg gttctttaac ttctccttta tctgcatcaa
     8581 cagcattttc ccaagaagct ggaacttcaa ctttaactaa agcatttatt cctgcatcaa
     8641 cagcagcata gttcatatta actatttttt cacctttttt accataagct ttaactatag
     8701 aatcttttaa gtatttaaca gcatcttctt ctggtattat ttcagctaat ttaaagaatg
     8761 cagattgcat tatcatgttt attctgtttc caagacctat ttcttgtcct agtttaacag
     8821 cattaactgt atagaatttt atatcatttt tagctatata ttgtttcata tgagctggta
     8881 aatgttcttc aagtcctgct tggtcccata tagtgttaag tacaaatgta ccaccttttt
     8941 taagaccttt taataaatca tattgataaa catatgattg tttatgacag gcaatataat
     9001 cagcttcatc tattaaataa gtagctctta ttggagtatc accaaatctt aagtgagaca
     9061 tagttatacc accagatttt ttagagtcat aatcaaagta agcttgagca tatttttcag
     9121 tatggtcacc gattattttg atagcttgtt tattagctcc aacagtaccg tcagaaccta
     9181 atccccagaa cttacatctt atagttcctt tagaagctat ttttattggc tcagatggag
     9241 ttaaagaagt attagttaca tcatcaacta tacctactgt aaatccattt ttaggttgtt
     9301 ctgaaactaa gttatcaaaa acagttttta tatctgaagg agtagtatct tttgaaccta
     9361 atccgtatct tccacctatt atcataggag cattttcttt tccatagaat acatcacgta
     9421 catctaagta taatggttca ccagttgaac ctggctcttt agttctatca agtacacata
     9481 ttctttcaac agtagatggc atagcatcta agaaatgttt cattgagaat ggtctatata
     9541 aatgaacttt aactaaacca tatttttctc ccttagcatt taagtaatct atagtttctt
     9601 ctattgtttc tgttactgaa cccatagcta ttaatacgta tttagcatct tctgctccat
     9661 agtaatcaaa taaactatgt tttctacctg ttaaattact cattttttcc atgtattttt
     9721 caactatacc tactatattt tgatagtatt tattagaagc ttctctagtt tggaaatata
     9781 tatctgggtt ttgagcagta ccacgagcaa ctggatgatt tggagataaa gcattatctc
     9841 taaatgcttg aactgcttca aagtttaata aacttgcata atcttcattt tctagtaatt
     9901 caactttttg gatttcatgt gaagttctga atccatcaaa gaaatgtata aatggtaatc
     9961 taccttctat tgcagctaaa tgtgcaacag gtgctatatc agctacttct tgaactgaac
    10021 cagaagctaa taatacacat ccagtttgtc tagcagccat aacgtcttgg tgatccccaa
    10081 atattgataa cgcttgagat gccaaagcac gagcacttac atggaatact cctggtaata
    10141 actctcctgc aactttatac atgttaggta tcattaataa taaaccttga gatgctgtaa
    10201 aagtagaagt taaagctcct ccttgtaaag aaccgtggaa tgttcctgat gcacctgctt
    10261 ctgactgcat ttcaacaaca ttaactgttt gtccaaatat attttttctt ccttgtgatg
    10321 cccactcatc aactacttca gccatagttg aagatggtgt gattggatat atagctgcta
    10381 catctgtaaa cgcataggca acatgagctg cagctgtatt tccatcaagt gttttcataa
    10441 acttagccat aatatgtatc ctccttgata gctacaattg tacttgttta taacaagtct
    10501 atttttttat atttacatat aatttacaca acaaaaatat aaacacacat atagtcgcgc
    10561 aaatactcta tcttctaata taatactaca gattagataa aattcctttg aaaattgtaa
    10621 attaattaca tatttaagta gatttttttt aaaattaaat atgttaagtt tttttcataa
    10681 ttttgtatac aatatataat aaattaagtt ag
//
LOCUS       DAEPOG010000131        10593 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEPOG010000131 DAEPOG010000000
VERSION     DAEPOG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.131"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            47..544
                     /locus_tag="KR432_002747"
     CDS             47..544
                     /locus_tag="KR432_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH0641268.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            1288..1650
                     /locus_tag="KR432_002748"
     CDS             1288..1650
                     /locus_tag="KR432_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0641269.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            1716..2333
                     /locus_tag="KR432_002749"
     CDS             1716..2333
                     /locus_tag="KR432_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBH0641270.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            2330..2815
                     /locus_tag="KR432_002750"
     CDS             2330..2815
                     /locus_tag="KR432_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBH0641271.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            2858..3376
                     /locus_tag="KR432_002751"
     CDS             2858..3376
                     /locus_tag="KR432_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBH0641272.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            3512..4471
                     /locus_tag="KR432_002752"
     CDS             3512..4471
                     /locus_tag="KR432_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0641273.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            4497..6200
                     /locus_tag="KR432_002753"
     CDS             4497..6200
                     /locus_tag="KR432_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH0641274.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            6457..8559
                     /locus_tag="KR432_002754"
     CDS             6457..8559
                     /locus_tag="KR432_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH0641275.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            8572..9012
                     /locus_tag="KR432_002755"
     CDS             8572..9012
                     /locus_tag="KR432_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH0641276.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            9048..9851
                     /locus_tag="KR432_002756"
     CDS             9048..9851
                     /locus_tag="KR432_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH0641277.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9891..10487
                     /locus_tag="KR432_002757"
     CDS             9891..10487
                     /locus_tag="KR432_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBH0641278.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
BASE COUNT         4279 a         1018 c         1973 g         3323 t
ORIGIN      
        1 agttcaaaat aaaatatatt aataaaaaca aagtgaggta taaagtatga attatgaaat
       61 agtagagata aatgagaaaa tagttatagg aatatcgaag gagactacaa ataaagatgg
      121 acaagcggta aatgctatag gtgaattgtg gaaaaaattt atgggcaaag ggatatataa
      181 tgctataaag ggtaaaaaga atgacaagac aataggtcta tatacagatt accagggtga
      241 ttttacaagt ccgtatagct ttgttgcatg ttgtgaagta aatagtaatt ctgataaaga
      301 gaagaattta gaagaattaa acactaataa tactgttgga gaaagtatta ttagtaaagt
      361 tatactagca ggtaagtatg caaagtttgt tatagtagga ggtcaaaaag aagtaggaga
      421 tttttggttt aagttttggc aaatggattt tgataggact tatataagtg attttgaaga
      481 gtatcaatgt aatacttttg atacaaagaa acaagaaatt catatttaca taggaataaa
      541 gtagatttgt tattactata ttgaatgaag tattgagtat agtacattta ttatagatag
      601 ttttactatt tataactata agcagtaaat ctgtaaaaga tttactgctt ttttagtaat
      661 taatagtatc agtatcactt tgaatcttca gacatattcg tcatttaata ttgtttattg
      721 ataaatgata cttgtaaata taatgatttg ttatttaatt taaaatatga aaaaaagtat
      781 tataaacaca ttagaatact tctggattta tgagtataca atttgaaaaa tataaaaaat
      841 aatgtataaa gctaaatcta atttaatcga taatacatat agagaaatat aattaagtaa
      901 ataaacaact atctattaga tattgagaaa gtcaaatcct atatgaatct acagaaatga
      961 ttttaatgaa tctgaaaaaa ttttttatac aaacataaaa aaggtataaa gttgttttat
     1021 tataatttga ttacttttga taacttttta tgttttatac aattatattt ttaataactc
     1081 gtcaaaacgc caaacatata ttacaataga tatataagtt tttttgttga caaattatgt
     1141 taagagaatt aagtataata tggcataaat aattactttt atttatcaat gaaatgtatt
     1201 tattgtgtag cgaaaaatag tcagatagta tagacaataa ctgaagataa aagcgtttat
     1261 tgttgtacat atggaggagt tagttttatg gaaaataaaa ttttgatagt agatgatgct
     1321 atttttatga gaatgataat taaagaagct ctgagtaaaa gtggctataa taatttaata
     1381 gaagcaaatg atggagaaga agcgtgtaat atatttaaat atgaaaagcc tgatttgatg
     1441 ttgcttgata ttacaatgcc aaaaaaagat ggtttagagg tgctaagaga aataaaaaag
     1501 ttggattcaa gtgctaaggt tgtaatgtgc tctgcaatag gacaagagaa tatgattgta
     1561 gaagccataa aacttggagc gttagatttt atagtgaagc ccttcaaaac agaaacatta
     1621 gttaaagttg taaataatgt tttaaaataa aagtgtaaat atacactgat acaatgaaaa
     1681 gcaaataaat gcaataataa aagggagggg tttatattat aaattattta gaacttgatg
     1741 atgttcatat agatgctctt agagaaattg gtaatatagg ctctgggaat gcaattactg
     1801 cacttgcatc catgataaat agcaatgtag aagtattaat acctatggtc aagatacttg
     1861 aatataatga agcgacaaat ttgcttggtg gtccagaaaa caaagtagta tgtattttac
     1921 tcgatatgaa aggtgatatc aatggaatgt ttatgttttt gttggatgaa tcaataactc
     1981 aacttatgct aagttctctt tttaataaag aagaagcttt tttagatgag attgaggcaa
     2041 ttgaaatttc agcaattaag gaaataggaa atattatggc aagttcatat gtaaatgcta
     2101 ttgcatctat gttaaacatg actataagtg tttcaattcc agatatatgt attgatatgg
     2161 ttggagctgt tctaaatgtg cctatgataa gattttcaga tgttggtgac aaagtcttat
     2221 ttatagaaaa taaatttaaa atgagtgata attattttac tagccacatt ttgatgattc
     2281 cagaaatgag ctcacttaga gaaatactcg tgaggttggg attagaagta tgaatagaga
     2341 gatagtagtg aacattgcag atatgaagat agcctataga ccaaatgttt tagttactta
     2401 tgcacttggc tcttgtgtag gagtatgttt gatagataaa gtagctggaa ttggtggaat
     2461 gttacacgtt atgttgccat atagtaaaga tgctattaac attgaaaata agtgtaaatt
     2521 tgctgacaca ggaataaatg agctcattaa atctatggag aatataggtg caaatagaat
     2581 atatataaaa gcaaaaattg ctggaggtgc tcagatgttt ttaggaagca gcaattcagt
     2641 aattgcaagt ataggacata ggaatgttga ggcagtaaaa aaaactcttt taggattaaa
     2701 tattccaata ttagctgagg acacaggtat gaattatggt agaacaatac gtttttacac
     2761 tgagacgggt gaattacttg tagattcaat aaataaaggt aagaagaaat tataatatat
     2821 atttcataga aagtgtattt taggagggag gtattaaatg agatttccag atgacccata
     2881 tttagattat ggactaattg atgaaggaat taagtatcta aagtttaaag tagatgagca
     2941 agattttggg gttgaacttg aaaaggtaat agaaattact ggaaatcaag aaattatatt
     3001 tattccagaa ctacctacat attctaaagg cataataaat ctaagaggaa aaatcattcc
     3061 aataatagat atgcgtttga gacttaaaaa agatgaatta atgactacaa attgcatgta
     3121 tatagtagta actgaaataa aggacttggt agttggattt gtggttgata gagttgatga
     3181 tatagtaagc ttagaaaaaa gtaaaatatc acctccacca agagttacta tagaatattc
     3241 ggattatttt gtaagtggcg ttggagaatt agaaggtaaa ataattactt tactagattt
     3301 agagaaactt ttgacagata aagatgaagt tttaatagat gaattaatca atgggtttga
     3361 ttgcactatc atgtaacata gggatgtagt tggagtgtta ttataaaaag attgtgatga
     3421 gaacatttaa gaatatagtg gtataattat taagtataaa aattttaaat cattaaaatt
     3481 tgaatatatt atgtttaaga gaggtttgta tatgaaaaat aagcaacagg gaacaatttt
     3541 agtcgttgat gatagtgttc tgatgtgtga cttaatagat aaaacactta caaaagataa
     3601 ttttaaggtt aaaaaagcat ttaatgcaat agaggcaaaa gaatttattg aagaatttat
     3661 accagacatt attttgcttg atataatttt accagataac tctggttttg agttttgtaa
     3721 ggaagtaaaa tctaatactc gtacagcaga tatacctata atttttataa cttcaaaaaa
     3781 tacagataca gacatagtaa aaggatttgg tgtaggagca attgactata tggtgaaacc
     3841 ttttagtatg acagaattaa aagctcgtgt tatagctcat cttgaagtaa agaaatctca
     3901 agataaatta aaaaagataa ataatgaact ggaatcttct cttgaaaaat tgaatagact
     3961 cgttgtaaga gattatctta caggtcttta caataggcga catataatta atcagcttat
     4021 ggagcaaaga agaattaata aatatagtga aacatcaata tcccttatcc ttggtgatat
     4081 tgatgacttt aaggttatca atgatactta tgggcatgaa gcaggggatt tcgtgttaac
     4141 agtaatatca agtataataa aaaagaattg tagacaaatt gatactgtat caagatgggg
     4201 tggagaagag tttttgataa tgctcacaaa tactccaata gaaggtgcta agatactttc
     4261 agaaagaatc cgtaaagaga taaaggagtt tgatttcaac tataaagaac ataaaataaa
     4321 gtgcactatt acattaggag tagtagaagt aaactcaaat atatcattag aagaaaacat
     4381 atccttagca gataaagcaa tgtatgaggg taaaaactta ggaaaagatt gtagcgtagt
     4441 gagtacaatg aaaggattaa aaaagatata aacttacttt atgaaggagt acagatatga
     4501 aaagagaagt aaagctttct aaattagcaa ttgtattgct gattattata gtattggctg
     4561 ttctagcagg gattttttct tctatttcat cttataaagc atttgaaaaa gtagaaaaaa
     4621 gtattaatga tatgaataat ttgaggactc aaagtcagta tataaaagat acaactagag
     4681 agataagtga tgtattaaga agatatgtat ttacaggaga aaaaaagaat aaagatattt
     4741 atgaaaaaga attatacgga aaaacaagag aatcagcttt aaacaaatta gaaaaaattg
     4801 gtcttacaaa gagcgagtca aataaaatct tagaagcaaa agaaatatca gattctttag
     4861 taccaacaga acaaagtgca ataaaggcag tcgaaagtaa tgataagagt aaagctcaaa
     4921 gtataatttt tggtagtgac tatataaact cccaagagaa aatggattct ttaataaatg
     4981 aatttcaaga ggatatagtt acccgttcaa attccaaaat tgtagaagta aaaaaagaat
     5041 tacaaagtct aatattaaga acaatattta tactttccct tttaataata ttgacaataa
     5101 tagaatatac agtaataaaa ttaaagatag tgattcctat taatagagta gaaaaacact
     5161 tttcaagaat tgcatctggg gacttaagaa caagtataga cgttgaagaa agtaaatctg
     5221 aaataggact attagtgagt tctgtcaaaa agatgcaaag tatgctttta aattacatag
     5281 accttataga cagtacatta actagtattg caaaaggaga tttaagagta gagatagacc
     5341 aagaatttat aggtgatttt aaatcaatta aaatatcttt agaaagtata ataaactctt
     5401 ttaatggtga tttttctgaa ataaatttat cagcagaaca agttgcaagt gcttcagaac
     5461 aagttgcagc aggtgcacaa gctctttcac agggtgctac tgaacaagct agttcagtag
     5521 aagaattagc ttccacgatt aatgagatat cagatggaat taagaagtgt gcagatagtg
     5581 ctagtcttgc atgtgaggta tctgaccaat ctgcatcaca agttggtttt ggaagtagat
     5641 gtatggatga tatgatgaca tcaatgggtg aaataagtga aaaatcgagt gaaatatcaa
     5701 aaataatcaa aacaatagat gagatagcat tccaaactaa tattctcgca ttaaatgcag
     5761 ctgttgaagc agcaagagca ggtcaatatg gtagaggatt tgcagttgta gcagatgaag
     5821 ttaggtcttt ggctggtaaa agtgcaaaag cagctcaaaa tacagctatt ctcatagaag
     5881 aagctattaa aacagtagaa aatggtgtca gaattgctaa agagactgca aatgcattgg
     5941 acttggtagt aaatggtgta gaaaaaacta ctaattatat taatggtatt tcttcggctg
     6001 tacatagtca agaagtagca attacacaga ttttatctgg aatagagcaa atttccatag
     6061 tagtccaaat gaactcagca acagcagagg aaagtgcagc agcctcagaa gagatgtctg
     6121 ctcaagctca aattttaaaa gaattagtag aaaaatttca tctaaaggaa agtgaagtaa
     6181 gagaatcatc atgggtttaa attatgaggt gaatattgaa gtaagagaat tatcatgtat
     6241 ttaaattatg aggtgaatat tgaagtaaga gaattatcat gtatttaaat tatgagatgt
     6301 atatatattg aaataagaga atgattgtgt atttaaatta tgaggtacat atagtgagat
     6361 aagagaatca tcaaggattt aaattatgag atgcatatat aatagaaaat tatttgtagg
     6421 tcgtaggtga taaatgtgaa ttatattgaa ccgagtatga gacaaatgtt agatatgttt
     6481 atatttgaga caagtttatt actcgaacaa ttagatgaaa ttatgattca aatagaaaaa
     6541 gagaaagtat ttactcataa caacattaat gaaactttta gaataatgca tacgataaaa
     6601 ggttcagcat ctatgatggg gttagacaat atatctgctt tagcacatag tgtagaagac
     6661 ttgttttatg ctatacgtga aggtaaaata gaagttttag acaatgacct attatttgat
     6721 ttagtctttc aatcattaga ttttataaga agagatatag atgagctaca atcaagaggt
     6781 tgtatatctt ctgattactc taattttatt gaaggaattg aagagtatgt gagtactttg
     6841 gaattatcaa gttctatgaa atcaaaggat aatgcaaatt tagaaagtac agacgaaaaa
     6901 gaagcaatcg attcaaattt tgaatacaaa gaagactctg atgtagtaaa aataatcttt
     6961 gaacaaggtt gcatgatgga aaatgtgaga gcgttcatgc ttgtatttga acttagagat
     7021 tactgtgaat ttatagattt ttatccaaaa gatgtagaaa caaatgcaga gtctacagaa
     7081 tatattaaaa gttatgggtt ttatatacaa tttgtagcta aagaggataa ggcaatagtt
     7141 tataaaacta tagaagataa tctctatgta aaagaataca taaatattag taaaaaagaa
     7201 atagaaatga aaaatacatc aaataatgat gcagtagaaa ataaattaaa agataataat
     7261 aaagtaagta aaagttatca aaataactat acagaagatg agatacaaga agatgtttta
     7321 aatgaaaaaa ctgatatgga aaaagatagt ctaagtgaag aaaatgaaca tttaccaata
     7381 agtaagcaga gtattatgag tgtaaattta gaaaaattgg atttacttca aaatatagtt
     7441 ggagaaattg taattatgga atcaatggtt gtaaatagcg taaattctac aggtgttgat
     7501 atagataatt tcaataaggc aacaagacaa ttacacaagc ttacagatga attgcaagat
     7561 atagtaatgt ctatgcgtat gattcaaatt gctggtgtat ttcaaaaaat gcatagaatt
     7621 gtacgagata tgaataaaaa attagataaa aatgtagagt taataactat aggagcagag
     7681 acagaggtag ataagaatgt aattgataat ttagcagaac cttttatgca ccttataaga
     7741 aatgctatgg accatgcaat agagcctatt gaggagcgta tagcgaaagg taaagaagaa
     7801 gtgggaaaga tagttttatc tgctgagaat ctaggcggag aagctgtgat aagtatttca
     7861 gatgatggta gaggcattat gagagataag attttggcaa aagctaagga acaaggtctt
     7921 cttaaacatc aaattaatga gtatactgat gacgaggtca attctttgat tatgactcca
     7981 ggtttttcta caaaagaatc tataactgaa ttttctggaa gaggagtagg catggacatt
     8041 gttcgtaaaa gtattgaaaa agttggaggt tcaattgaag taaaaagtaa acaggatgaa
     8101 gggacaacct ttactataaa aataccatta actctttcta tagtagaggg tatgaagttt
     8161 aaagttggag attcactatt tactttacca atatcatcca taagaagaac atttaaactt
     8221 acagacttaa atcaaataat tacagaagga aataagacag agttaattac aatatatgga
     8281 gcatgtattc ccattataag gttacataaa atatataata ttcaaacaaa agtaaccaat
     8341 ctgatgcatg gtaatatgat aattattgaa aatgagcata aagcagtatg catttttgtt
     8401 gatgaaataa taggtaatca gcaagttgtt gtgaaaccat ttccagttta tttcaaccga
     8461 tttaatataa aaaataaggg tttatcggga tgtacaatac ttggggatgg aagtataagt
     8521 ttaattatag atgcagatag cattatagaa atatattagg ggatttaaag tatggctaaa
     8581 gatgaaatta ttaaacaagt attaacattt tatgtaaatg atgttattta tggaatagaa
     8641 ttagaaaatg tgattgaaac cataagattt caatcaataa cgtatgtacc atgtcttcca
     8701 acatatataa gtggagttat taatttgaga ggaagaataa taccagttat taatatgcat
     8761 ataaagtaca atcttcctaa agctgattat aatgagagaa cttgtataat aattacaaag
     8821 gttgatgagt atcaagttgg aattattgta gataaagttg ttgatgttat tcatatagaa
     8881 aatctaaatt tactagagac gactaattct aacaacacta atattaataa agatataaaa
     8941 gagattgcga aagttgaaga taattctata ttaattttag atattcgaaa gtttttgatt
     9001 aatagcatat agttagttct gataaattag caggaggaag tttttgcatg ataaaactca
     9061 cagatgaaga atttatagtt cttgtaaacc atgtaaaaaa agaatatggt attgatttaa
     9121 gtaaaaaaag agcgttaatt gaaggtcgct tatacaatac aatgatagag aaaaaactta
     9181 gttcttttag tcaatatatg aatctattat ttaaagataa gacaggaaat gaagctataa
     9241 acctaataaa tagattgagt actaaccaca cattttttat gagagaacct caacattttg
     9301 agtttataca gaatagtata cttccttttt gggaagaaaa taataaaata agaaacataa
     9361 atatatggag tgcaggatgt tctagtggag aagaagcgta ttcaatagct atgactttgg
     9421 atgattattt tggatataat aaagagttgt ggaacattaa aatcatagcc acagatattt
     9481 caactaactc acttgaaaaa gctaaaagag gtatatacat agaatcaaat gttaacaatg
     9541 ttcctgagtt atggaagaag aagtatttta tagacaacaa ggatggtaca tttaaaattt
     9601 gtgataagat tagaaaaagt gtaattttta agtcatttaa tttaatgaat gatttctcat
     9661 atcaacattt tgatttgatt ttttgtagaa atgttatgat ttattttgat ttaaaaacaa
     9721 gagaagaact tataaataaa ttttataatg ctaccaaaaa agatagtttt ttgtttgtag
     9781 gacatgcaga agtaatcaac agaagtagta ctaaatacaa atatgtaaaa cctgctatat
     9841 ataaacgata aaaaggtatt tttgatatat gattttttgg aggcggtaat gtggaacgtt
     9901 ttaataatat ggttattgct ataggcgcat ctgtaggtgg tacagaagca atacttgaaa
     9961 ttataaaaga cctaccaaaa tcaacaccag ggattgttgt agttcaacat atgccagcta
    10021 tatttactta tatgtatgca caacggcttg acaagcagtg tattatgaat gtaagagaag
    10081 ctaagaataa tgatagagtc gaacaaggaa atgtgttaat tgcacctggg ggttaccaga
    10141 tgaaattgtg tacggataaa caaggatatt atgtaacttg tgaaaaagga gaaagaataa
    10201 gtggtcactg tccatcagta gacgttttgt ttgattcagt agcagaagta gcaggtaaaa
    10261 attcaatagg aataatactt acaggtatgg gttatgatgg agcaaatgga cttttaaaaa
    10321 tgaaagaaaa tggtttattt acaattggac aggatgaaaa ttcttgtata gtatatggaa
    10381 tgccaatggt agcttttgat agaggttctg ttatgttaca attacctctt ggtgaaattt
    10441 caaactgttt gataagacat attaatattt taaaacaaaa agattaattt tttataaata
    10501 aaaattaatc ttttattaaa ttatatggat aaaaaaccct agaaactcca aatataataa
    10561 gagagattta tatttaggag gaaaatgata ata
//
LOCUS       DAEPOG010000132        10587 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEPOG010000132 DAEPOG010000000
VERSION     DAEPOG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.132"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..264
                     /locus_tag="KR432_002758"
     CDS             <1..264
                     /locus_tag="KR432_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641279.1"
                     /translation="KKVDKIDDIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVI
                     TVAIQQGLVSFGAFRNKNYVKALFSIFVIVCFLIAFKYVVNPV"
     gene            356..1816
                     /locus_tag="KR432_002759"
     CDS             356..1816
                     /locus_tag="KR432_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0641280.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            1869..4745
                     /locus_tag="KR432_002760"
     CDS             1869..4745
                     /locus_tag="KR432_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0641281.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            4822..6888
                     /locus_tag="KR432_002761"
     CDS             4822..6888
                     /locus_tag="KR432_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0641282.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    7480..7690
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7916..8731
                     /locus_tag="KR432_002762"
     CDS             7916..8731
                     /locus_tag="KR432_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0641283.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            8759..9436
                     /locus_tag="KR432_002763"
     CDS             8759..9436
                     /locus_tag="KR432_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0641284.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            9495..10217
                     /locus_tag="KR432_002764"
     CDS             9495..10217
                     /locus_tag="KR432_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0641285.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         4328 a         1015 c         1882 g         3362 t
ORIGIN      
        1 aagaaggtag ataagattga tgatattatt aataaatcta gtattgtatt atgtgtactt
       61 ctagtggtgt tttcattact ttacttattt aaattggttc aacttaatat aataatgata
      121 cttttagtaa ttacagtagc tattcaacaa ggattggtgt catttggtgc atttagaaat
      181 aaaaattatg taaaagcttt attttctata tttgtaatag tatgtttctt aattgctttt
      241 aaatatgttg taaatcctgt ttaaatatat caataaacgt tctataatta atataaatag
      301 tgaagttaca gttttaatgc tattataaat tttataattt gaaggagtag attttatgaa
      361 taggtgtaag tttgcaaaat taattatcac atcaatgatt gttttattac ttacaggatg
      421 tagtttaaaa aaggaagata attataagtt agacccagaa aatccgacat caatacaaat
      481 ttggcattat tataatggac agcaaaaaat ggcttttgat aagcttgtta aggaatttaa
      541 tgacactata ggtgctgaaa aaggaattat tgttgaagcg tttagtcaag gtaatgtagt
      601 tgatttatca gagaaagttt tagattctgc aaataaaaaa gttggctctg aagatgtgcc
      661 aaatatattt gcagcatact cagatactgc atatgaaatg gataaattag ggttactagt
      721 agatatggat aagtacataa gtaaagaaga attaaatgaa tacatagacg aatatgtaga
      781 agaaggcaga ataaattcta aggatgagtt taaacttttc ccaattgcaa aatcaactga
      841 aattttgatg ttaaataaaa ctgattggga taaatttgca aaagctacaa attcaaatat
      901 aaatgatata aaaacaattg aaggattagt agagacatca aaaaaatatt atgaatggac
      961 agatagttta acgaaagagc ctaatgatgg taaagcattt tttgggagag atgctttagc
     1021 aaattatatt attataggaa gtaaacaact aggagtagaa attttttctg ttaaagatgg
     1081 taaagttaca ctaaatattg aagaaaaagt tatgaaaaag ctatgggata atttctatgt
     1141 accatatata aatggatatt ttgcttctta tggtaagttt cgttctgatg atgcaaaaac
     1201 aggtgatata ttagctcttg taggctctag ttcaggagca gcatactttc cagatagagt
     1261 aatgctaaat gataatgaga gttataatat tgaaacatct gtttttcaag caccacgttt
     1321 taaaggagaa ggaaaatatg ctgtacaaca aggtgcagga atggctgtaa caaaatctac
     1381 taaagaagaa gaatatgcat cagtagaatt tttaaaatgg tttacagata aagagcctaa
     1441 tataaaattc tctattgctt ctggatattt accagtaaag aaaaaaacga atgatttgaa
     1501 tgttataaaa gaagttatga agaaagataa tttgagttta tctgataaag ttgaaggttc
     1561 aatagcctca tctattgaac aaattaacag ttgtaattta tacactaata aagcatttga
     1621 tggtggaact aaagctagac aaatacttga atattcaatg cttgataaag ctaaagaaga
     1681 ttcaaataaa ataaatgaat tggtaaaaag tggaacttct aagaaggatg cggtagcaca
     1741 atttaataat gataagaatt ttgaaacttg gtttgaaagt tttaaggctg agcttgaaaa
     1801 tgctataaaa aaatagatga attggcagtt agtgtgtaga caagcacaaa ggtataggtg
     1861 gaataagtat ggaaaacaat ctaagaaaaa acaattcaat aatgaaaaag atgatatttc
     1921 ccctaattat tgttatgcta attcaaacaa gtttattttg tgccacaatt ttatggggag
     1981 gaactattaa gaaattaaat gataattcgt ttgacatctt aaatgaaaga gtaattaatc
     2041 gtaaaaatta tatacaaaat gaaatgttac aaagatggtc aaatacaagt gaaacggaag
     2101 ctacaattaa ttcctctgta aaaaaaatat taaatgaaaa aaatgcctta attgaggatg
     2161 taggcaacaa tgaagaaatt aacaaagata ttataaaaag catatcaaat gatttaatat
     2221 atcttttgag aaaaaactct gttacaggag cttttataat tctaaatgac aaaaatatta
     2281 acgataagcc aatagatagt aaaggtgaaa taagtaggac tggattgtat ataagggatt
     2341 tagaccctaa gtttaatcca aatgataatt ctgatttgtt aattgagaga ggttcatcag
     2401 atatagcaag aagttttgga atatctatgg acagttattg ggaacctaaa ttttcattta
     2461 aaagtgaaaa tgagcaagag ggagatgatt ttttttataa accatttaga gctgcaattg
     2521 ataatccaaa cgtatcaagt tctaatttgg gatactgggg aagtaagttt ttattgacaa
     2581 atgcaggtga taggcttatg acatatacag ttccccttat ttatgaagat ggaactgtat
     2641 atggagtact tggaatagat ttaagtgtaa attatttaag agaattactt ccatactcag
     2701 agataaatgg aaataaaaaa ggtaattata ttttaggtgt agatagaaat aatgatatgg
     2761 cctttgaaaa tgtaatttca aatggatact cattaaaaga aaaattttgg gatggtttac
     2821 attctaattt agagcagaag gaatcgtata aaaatatata taagattaaa aataaagata
     2881 atgaatgtgg taatggcaac atttatgcta gtgtacaata cttagattta tataactcca
     2941 atactccatt tgaaaatgaa cgttgggctc ttttgggtat tattgaagaa aaaagtcttt
     3001 tgtcattttc aaaaaggata gagaaattag ttataatatc tatagcaatt tctttattaa
     3061 taggtctaat aggtatttac attgcatcaa gattatttac aaaaccaatt gttcttttag
     3121 ccaacaaagt tagaaacagt aatccatcaa aaccagtaaa tcttgataag ataaatatat
     3181 ctgaaataga tgaattatct tcagctatag agttactcag tgcaaatgtt gcagattctt
     3241 catcaaaatt atcacagatt attgaaatgg tagatatgcc aatcggggct tttgagttat
     3301 acaaaaacgc agagaatgtt ttttgtacaa aagataaaaa acttaagtgg ataagaatga
     3361 atatctttga agatgagcta aaggttcttg gtgttgttgt tgatgtcaca aaagaattta
     3421 ttgagaaaag gaagatagaa tatgaaagag attttgatat tttaacgaat cttttaaata
     3481 ggagagcttt tcattcacag atacaagaaa aatttaagaa taaagatgaa cttaagatag
     3541 cagcatttat tatgtgggat ttagataatc taaaatatat taatgataca tatggacatg
     3601 atgctggtga tgaatatatt aggtgtgctg cagacatact taagaagttt atatcatgga
     3661 atggtatagt atctagactt tcaggtgatg agttttatgt atttatatat ggatatgaaa
     3721 gtaaagatag tataagagat attattaatt ctgttaagaa acagatgaat aataccttat
     3781 taaaacttca tgatggaaca gaatttaaaa taagggcatc agcaggaatt tcctggtacc
     3841 ctgatgattc aaccaactat gatgaattaa tgagatacgc agattttgct atgtatcaaa
     3901 ttaaaaatac aatcaagggg gaagttagtg aatttgatat taatatatat aataaggatt
     3961 cattcttatt gagtaataga gaagaattaa acaagttaat tgatggtcaa ttagttgagt
     4021 atgcatttca gcctattgta gattgtaaag gtaaagtatt tgcatatgag gcacttatga
     4081 gacctaaaat aaaaactttt agttcaccaa tagatgtaat tagattagct cgttcacagt
     4141 ctaaattata tcaaattgag cgtattacat ggtttaaagc acttaaatca tttaaaaaat
     4201 acaaagaaaa atttggcgat tgcaaactgt tcttaaattc tattcctaat aataatttat
     4261 caaaagtaga ttttgaggaa attgaagatt tatatggtga atatttaaat agagtagtat
     4321 tagaaataac agagaatgat aagattaatg aagattttat tgagaaaaag aaagcatata
     4381 tagaccattg gaatatagaa attgctttag atgactttgg gacaggttac aacggagatg
     4441 ctgtactatt atatataatg ccagactatg taaagattga tatgtctatt gtaagaggta
     4501 ttgataaaga tgaaaacaga cagaaaatat taaaaaatct tatctcatat tctaacgaaa
     4561 gaaatattaa agtgatatca gaaggtgtag aaacaaaagg tgaaatggaa acactgatta
     4621 atcttggagt agattatatg cagggttact atattagtaa accatcattt atacctgaag
     4681 atataagtga caaaattaaa aaagagctca agatatgtca tgaaaatagg aatgatgtgg
     4741 tttaatagag gaaaaattaa atttattttt atgaactttt agtttataat taaaaagaaa
     4801 ataatataat tataaattaa ggtggtggag ctaataatca caacaaacat agatataaga
     4861 aagttaaatg aaaatcaatt agaagcagtt gaacatatag atgggccttg catgatactt
     4921 gcagggcctg gttcaggtaa gaccagagta attacatata gaatagccaa tatggttgta
     4981 aacaaaaata tagcaccaac aagaatcttg gcaataagtt ttaccaaggc atcttcaata
     5041 gaaatgaaaa atagagcttt gaatttaagt gatgatataa gacttaataa ggtaacctat
     5101 ggtacttttc actctgtatt ttttaaaatt ctaagatact ttgaaagata caatttagac
     5161 agtatatttg atgaaaagtc aaagcgaatg acaatcaagg caatcctaaa aagtttaaat
     5221 atagagaatg ctgatgatga tgagaatata ggacaagtta taaatgaaat ctcctatgta
     5281 aaaaatgaac ttatggataa aaatgaattt aattcagaag tattaacaaa agatgagttt
     5341 ttaaaagtat acaacttata cgaagaacaa aaatctaaag ttaacaaaat agattttgat
     5401 gatatgctta taagaactta ttatttactt ttgaacaata agtctgcact agaaatggtt
     5461 agaaatgtgt ataaatatat attgatagat gaatttcaag atataaataa agtacagttt
     5521 gaagttttaa agttaatttg tagcccatta aacaatatat ttgcagttgg agatgaagac
     5581 caaagtatat atggatttag aggtgctaga ccagattttt tgcttgaatt tgaacaatac
     5641 tttaataata ctaaaaaaat aatattagat ataaattata ggtctaaaag tgagatagtg
     5701 tatactgcaa atagacttat cgacaaaaat aaaaatagat atgaaaaagt cattaagtgt
     5761 agtcaggggg atggaggaag tgttacctac atttctccac atgattcaga ggaagaagcc
     5821 ttatatattg ccaaggaaat aattgacgaa atacaaaaag attatgtaga gtattcagat
     5881 tttgcagtaa tttatagaac aaatatacag tcaagagcat tagtagatgt atttatggat
     5941 atgaggattc catttgtagt aaaagactca gtaattacaa tatatgacca ttgggcaagt
     6001 caagatatac tagcttattt aagaataggt attaatccaa aatcaaataa agattggttg
     6061 agaattataa acaagccttt tagatatata tccaaagaca gtataaatat ggttaaggat
     6121 gaaaaagact ttataacagc attaataaat aagtgtaatc ttcatcctaa acaagttaaa
     6181 acaataaatg atttggaaat agatttaagt tatttaaata ctttaaatcc taaaaatgca
     6241 atatcttata ttagaactag tcttgattat gatagatacg ttttagatta ttgtagcaac
     6301 agaaagataa aaactaatgg acttgtagag atattaaatg aacttgagag ttcagcaact
     6361 aattttaata ctgttacaga gtttttagag catattgata gagtaaagac tgagttaacg
     6421 gaaaataaca agaataagca gactgagggt gtcatattta caactatgca tagtgcaaaa
     6481 ggattagaat ttagaaatgt ttatattata ggtgtaaatg aaggaacaat acctcatgaa
     6541 aaatcatatg atatttgtaa ggaagagaaa aaagaagaac aattagaaga agaaagaaga
     6601 cttatgtatg ttgctattac aagagctgaa gaaaagttat gtataagctc tactttaaat
     6661 aagtatggta agaaagttga taaatcatta tttattaatg atataaaatc tccaacgaaa
     6721 aaagaaattg acagtattgg tgtaggggat aaggtatacc ataaaaaatt tcatgaagga
     6781 gaaataatta aaaaagatgg aattatgttt acaataaagt ttaaagatag agagcgaatc
     6841 ttggatttga aaacttgttt attgaagaat ataatttata ctatttaatt tagaatctaa
     6901 atttagatag aaatataatg tattatttat tatgtgcaag acaaatagat atgtgtaaaa
     6961 tgtattattt aacttattag aaaaagaaat aaggatttaa tatattattt atagttgttt
     7021 agaaaaagca acaatgtgga aaatattatt tagtattatt aaagaaaagc aacaatgtga
     7081 aagatattat ttagtgtaat ttaagaaaaa gcaacaatgt gaaagatatt atttagtatt
     7141 attaaagaaa gagcaacagt gtgaaagata ctatttagta ttattaaaga aaaagcaaca
     7201 gtgtgaaaga tattatttag tgtaatttaa gaaaagcaac aatgtgaaag atattattta
     7261 gtattgttta agaaaaagca acaatgtgaa agatattatt tagtattatt taagaaatag
     7321 ctaaaaatat gaaatatact atttataatt acaaaaataa tatcaataaa tataaacagt
     7381 agtatttaga taatttacaa gtaaaaatta ttaaaaaaat aaaaaactta ttgacaaaaa
     7441 tattataagg aagtacaatt gtattaacaa tttaaggaaa caaatccaat gatagagaaa
     7501 gtagcatata tggatttaca gcgagttagg ggttggtgta agcctagcaa tgaagtctat
     7561 gtgaagagag ctctggagga gattatcaga aactttagta tgataattgg tttacccgcc
     7621 ttaagggatt gagaggataa gtacttactt atcaaaaaga gtggtaacgc ggatataatt
     7681 cgtctcttag ctgtaaagct aagggacttt tttgatttaa aaataaaaat atccatacag
     7741 cttaaatgct aacagtcaca aattttgaaa gtaatataag tatttatcaa taagagtggt
     7801 aacgcggtaa agtcgtctct tagtcattaa agactgagag actttttttg taactcgaaa
     7861 catttaagta ttaaaatctg agggaaatta taattaaaag agaggggtag ttaaaatgaa
     7921 agtatttaaa aaattattaa gtttaggatt agtattagga ttgacattat cgctagtagg
     7981 atgtagtggt ggaggagaaa aaacaaagtt agaacaaata aaagacaatg gaaaattagt
     8041 tgttggtact agtgcagaat ttcctccatt tgaatttcat aaggtagtag atggaaaaga
     8101 ttcaataaaa ggattcgata taatgttggc tgaagaattt gcaaaagagc ttggtgtaaa
     8161 ggttgaaatt aaggatatgt catttgatgg attaataggt gctttaaatg cagaccaagt
     8221 tgatattgtt cttgcaggta tgtctccaac accagagaga gaaaagagtg ttgatttttc
     8281 agaattatat tatttaagta gaaatgcagt tatagttaaa gatgcagata tagacaaagt
     8341 aaaaactgaa gatgatttaa agaaacttag agttggagtt caagcaggaa gtatccaaga
     8401 agaatatgta gttaacactt taaaaatgac aactacaaaa tctttaaaag caatacctga
     8461 tttaattaca gaattaaaaa atggaaacat agatgcagtt gttactaatg aagcagtttc
     8521 tttaataaat gttaaaaaat atgatggaat aaaaatggca aatactgaag ttggtaaaga
     8581 tgtaacagaa ggtatggcag cagcaatcaa aaaatctgac aataacaaag attttataga
     8641 attattaaac aaaaagataa aagaattaca agatggtaag aagatagaag aatttttaaa
     8701 tgaagcatct actgaagctg cttcaaatta gtaagaaagt tatgtgggag atgaaattat
     8761 gagtttagat tttagttttt taagtagatt tgggacttct tttttggaag gaacaggtgt
     8821 gacagtatca atttctcttg tggcattatg ctttggattt ataataggta taattatctg
     8881 tatggcaaaa atatcaaaga gcaaagtatt aagagcaata tcatcaatct atattgaggt
     8941 tttaagaggg acaccattat tagtacagat atatatagta tggtttggtt tacctcaatt
     9001 aggaataaga ttccctatgt tgtttggtat accaagtgaa tttatagcta gtgcatttgc
     9061 attatcagta aactctggtg catacgtggc agagatactt agatcaggta tacaatcagt
     9121 tgataatgga caaatggaag caagtagatc attaggatta aattattggt caactatgag
     9181 atatataata atacctcaag ctataaaaaa tatattacct tcattagcaa atgagtttat
     9241 tacacttgta aaagaatctt caataatatc tgtaatagga gttgttgaga taatgcgtac
     9301 tgctgatata gtaaaaaatg cagcatttag agcattagag ccattaatag tagcagcagc
     9361 aatttacttt gttataacat ttactctatc aagattagtt ggattattag aaaagaaact
     9421 atctgtgtct aactagatga gagcatgttt agcatttagg gaaaacatag aatagtaaaa
     9481 aggaaagtga taatatgatt acaataaaaa atttaagtaa atcttttgga gatttaaatg
     9541 tattaaaaaa tatagactta gaaatcgcta aaggcgaaat aatggttata gtaggtccaa
     9601 gtggttctgg taaaagtaca cttcttagat gtatgaattt actagaaata cctacaggtg
     9661 gagaaataat atttgaagga aaaaatttgg tcgataaaaa aactaatatt gatgaggtta
     9721 gacaaaatat aggtatggtg tttcaaaact ttaatttatt cccacataag actattttag
     9781 ataatataac attagcacca attaaattaa aaaagatgac taaggaagaa gcagaaaaaa
     9841 aagcagaaat tttgttgagt agagttggtc ttttagataa aaaagattca tatccatcac
     9901 aattatctgg aggacaaaag caaagaatag caatagctag agcacttgct atggaaccag
     9961 atatgatgtt gtttgatgag ccaacatctg ctcttgaccc agaaatggta aacgaagttt
    10021 tggatgttat aaaagaactt gctaaagaag gtatgacaat ggcaattgta actcatgaga
    10081 tgggatttgc taaggaagtt gcagatagag ttatttttat agatggagga agtatacttg
    10141 aagataatac tccagaagaa gttttcggaa atccaaaaca tgaaagaaca aaagcatttt
    10201 tagcaaaagt gctataatat ataaaaagga tgttattgat attagaaatt caatacatcc
    10261 ttttttagtg atggttaata ttaattttaa aattaaagtt atattaatat taactttatt
    10321 tttacgattt tttgcagaaa aaaaaagagc tcggggtaag ctcttttttt ccaccgaatt
    10381 aatttccgtt actaatccga tttttaataa atataaaata tatccttatg aaagggtatt
    10441 gggaatagat atattaacta actagggtta ccagggggat aaccttaatt tattataaca
    10501 aaatgcgaca caaaaatcaa tatttttttt gaaataaatt ataaaagtta tttttataac
    10561 aagtaagctt ataaatttaa cttttat
//
LOCUS       DAEPOG010000133        10404 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEPOG010000133 DAEPOG010000000
VERSION     DAEPOG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.133"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1425
                     /locus_tag="KR432_002765"
     CDS             <1..1425
                     /locus_tag="KR432_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBH0641286.1"
                     /translation="LSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMNTF
                     YNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNTTD
                     VINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEKWS
                     TASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEFNS
                     WFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSSTI
                     AFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIFID
                     PIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEWEK
                     HKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDIKY
                     NDEVIKANSLVIYDADSKVIYKPE"
     gene            1659..2150
                     /locus_tag="KR432_002766"
     CDS             1659..2150
                     /locus_tag="KR432_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0641287.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            2178..3125
                     /locus_tag="KR432_002767"
     CDS             2178..3125
                     /locus_tag="KR432_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641288.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            3415..3957
                     /locus_tag="KR432_002768"
     CDS             3415..3957
                     /locus_tag="KR432_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0641289.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            4282..5775
                     /locus_tag="KR432_002769"
     CDS             4282..5775
                     /locus_tag="KR432_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0641290.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            6012..6776
                     /locus_tag="KR432_002770"
     CDS             6012..6776
                     /locus_tag="KR432_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0641291.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            6797..6880
                     /locus_tag="KR432_002771"
                     /pseudo
     CDS             6797..6880
                     /locus_tag="KR432_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(7061..10240)
                     /locus_tag="KR432_002772"
     CDS             complement(7061..10240)
                     /locus_tag="KR432_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0641292.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
BASE COUNT         4120 a         1084 c         1516 g         3684 t
ORIGIN      
        1 ttaagtatga agtctggcaa tagtgaattt ataaaagtaa ttaatcaaat aaaaactcaa
       61 gattatatag ataaatatca aaaaaatcaa atagtacaag catttaatga agttatgcag
      121 atgaatactt tttataatga aaaagttttt gaaagcaaag atagagaaat aaaaaaagta
      181 ttagataaag gtatatctaa attaagtagg gtagaactta tagagtttaa taagaaatta
      241 ttgaaatcaa aattggaaga gagcgtgaat gatgttgcta catgggcaaa atataataca
      301 actgatgtaa ttaatgatat taataagtct ggtattaaaa atgcgtggat aggatttgat
      361 gatatggagg aaggttatat atccttagag atggttaaaa gagctaatga gttaggttat
      421 ctcgtagggc catatgattc ataccactct atacacaaac caggggaaga aaagtggagt
      481 acagcatcat ttaaagataa atctctatat gaaaatgcta ccatagaaaa taaaaaaggt
      541 gaaaaagaaa ctggatttca aggagaagga agaaaactaa atcctacatt gtctttacca
      601 agtgtaaaag agagagtaca aagtatattg gatgaaggat atgaatttaa ttcttggttt
      661 atagattgtg atgctacagg agaaatctat gatgattatt ctcaaaatca tataacaaca
      721 caaacacaag atttaaatgc tagacttgaa agaatgtcat atataagaga ccatgcaaat
      781 atggtcatag gttcagaagg tggaaatgat ttttcaagta gtactatagc atttgcacat
      841 ggtattgaga caccagcatt tgcttgggta gataaagata tgagtaagaa taaaggcagt
      901 gaatattatg ttggtaaata ctattctcca acaggaggag taccagaaat attttcaaag
      961 cagatacctg taaaagaaag gtacaaaaaa atatttatag acccaattta ctctctacct
     1021 ttatttagat tagtttataa tgattctgtg atatcttccc atcagtggtt atggggaact
     1081 ttgaagatta aggatgaagt tggaagtaga atgatgaaag aagtattata taatacacca
     1141 cctatgtatt atatagatag aactgaatgg gaaaaacata aagatgaaat aatctcaaac
     1201 aataaagtat ggtcagcgtt taataagaaa gctattaaaa aagaaatgac agactttaaa
     1261 tttatttctg aggatagact agttcaaatg actgaatatg gagaagattt aaaggtatta
     1321 gttaattttt ctcaaaaaga cattaagtac aatgatgaag tcataaaagc aaattctttg
     1381 gtaatatatg atgctgatag taaagtcata tataaaccgg aataatagat ttaatgaaaa
     1441 taaagattgt ttaaatttca agtaaaaaat aatataatat tcatttagtt tgtattaaag
     1501 tagtgactat attaatctgg ataattagta tagtcactat tatttatatt ctttcctaaa
     1561 agtaagtagt aactgaatcg tgaataatca aaaatggtaa tttgaaatag tttttatgaa
     1621 tagtattaat atatagatat agggagattg atgtgattat gtatgaaaat aataaggtaa
     1681 tatataattt tgaaagatgt gaaaagctag aaaatgctct ttatttatct caatttaata
     1741 catataaaaa ctttaagtta acttctaaaa aaactgtaat aaactcatta gatacatcta
     1801 cttataatat gaatattcat agcataagtc tcgttgatac tatggaatgt atatcgtcag
     1861 aagatgaaca aatgacagga aagaagttga ttttagttgg cgatatagat actgatgtaa
     1921 cattagatta tgcaggcaat aaaagaaata gaaattctag taaaaaaaat acatttaaat
     1981 taaaaatacc ctttagtaca tttatacaaa tgcctagaaa gatagaaaat aaagataaaa
     2041 ttaatttaaa gtatcttata caagatatta cttcatctat tttagatgat aatttattta
     2101 ttagtgttac tgcaattata agttatgaaa atagttccaa gatagagtaa tctttaaatt
     2161 atagaaaggt ggatgcaatg agaataacga aaactacttt taatgatgta ttttcaaatg
     2221 atgcaaatgg aagtattata aaagtagaaa aatatgttga taaaaaatgt gcatctgtat
     2281 ttgagatttt gacctacact attattgtta caaatacaag taggtacaaa actggaaata
     2341 tatttttcaa agattatatt tctaaatata tagagtttat aaataatact gtaaaagtta
     2401 atggcattat aaaacgtgga ttagacccac agcaaggttt ttacataggg agaatagatg
     2461 caagttgtaa aaaaataata tcttttaaga gtgtagtttt accaaattct gcacatagga
     2521 ttataaagaa tagtgctaat atatactatt attataagtg taatctagat aaattcccaa
     2581 caagaatatc aatagaaagt aatagagttt atactaatgt aaataaaatt gtattcaaac
     2641 aattgaatat aagtaatact ttaaaagctc ctaaaaatct aagagatata ttaaaagtaa
     2701 atgtaaatag taaaatattt gatgttaaac ctatgaaaaa tcttataaac aaaggtagta
     2761 atttgaaatt atgtaactcg atagtttttg ggtgtataga agttgaaata gactatagtt
     2821 gtaaaaatag gagtaatttt aaaacagtaa ataagtttaa tgatattaaa aaagataagt
     2881 attataaaaa tgatatacaa aataacaatg aaaatataaa cgattgtaaa aagcaaataa
     2941 gttttggtga taataaggta aaaaaaataa caaaaacttt tggattttct tgtttcattt
     3001 gttctccagt tggaattatt tatgaagata tgaaaaatat aaatataaaa attgaacata
     3061 catcaataaa tgaattaaat ccagaagaat tatttgtaaa tacatcactt ttattgtatt
     3121 attaagtatt ttaaagctaa aacttaaact tattaaaata gcatttggtt aaaggaaaat
     3181 agatattgat atatttataa tatatataaa tgattaatta aacaattata aataataagt
     3241 atagtataat atttaaaaaa tatagactta tgataaaata agacaaatat gttaaaaaaa
     3301 tgataatcac ggagaacttt taccttcttc aattaatata ttattattag taataacttt
     3361 aaaattatat tactattata taaaaattgg cttaaaagaa ggtgaaaaaa gtaaatgaaa
     3421 atatgcataa cagtaggtca tagtatacta aaaggtggta aaagtacagg tgtaaatggt
     3481 atagttgatg aatacaatta taataaaaaa ttagctccta tgttagctga gatgcttata
     3541 agtcaaggta atacagtaga tgtaataatt tgtccagaaa ggcactttat gtcagaaaga
     3601 gaagaatttt tctatagagt tccaaaagta aacagtggta aatacgattt attagtagag
     3661 ctacatttaa ataaagctga tggaacacaa tgtggtacag aagtgcttta ctatggcact
     3721 gaaggattag aatatgctaa aagagtatct aatagacttg gagaattatt tgaaaataga
     3781 ggagctaaaa aaagagaaaa tttatatata ttgagaaata caaatccagt tgcaatacag
     3841 attgaaagtt ttttctgtga taatgtgaat gattgtaaca aagcaaatga atcaggttat
     3901 gattatatag cacgattgat tacagaaggt atattgaata aagatataaa tatgtgagat
     3961 tatggtgatt taggtattga ttaatacctt ttaatattag atataaataa aaatacaatg
     4021 tattagtata atttaaactt tcaaaatata gattagtttg attattgaca agagatacct
     4081 cctaaatatt gatttatcag tatatttaag gaggtattta aatataaaaa tttaattgat
     4141 ttgctactaa actataggtt tatagagtat atatttttat cttcagtcta ataaataata
     4201 taaaaatagt agaaccttgt attataataa tataatgtag aaaaatatat agataagata
     4261 aatataataa ggtggtaata tatgaaatat acatttgaaa aatttaagtt aaatgaaaag
     4321 atacttaagt cattgaagag tttaggatat aatatacctt ctagagtaca aagagaagtt
     4381 attccaaaat tattaaaagg tcaaaactta gtagttaggt caaaaacagg aagtggaaag
     4441 acagtaagtt ttgcaatacc tttatgcgag aatataaatg tagattgcaa taatattcaa
     4501 gctcttatag tggttccaac aagagaatta gccttgcaag taaaagatga gatatcagat
     4561 atagggagac taaagaaggt gagatgtagt gctatatttg gcaaacaatc tataaaagac
     4621 caaatagctg aattgaaaca aagggttcat attgttgtag ctactccagg cagaatactt
     4681 gaccatataa ataaaggttc tattaagttg gaaaatgtaa agtatttagt aatagatgag
     4741 gcagataaaa tgtttaataa aggttttgtt gaacaaatgg aaaaaatatt attaaattta
     4801 cctaaggaga aaatagtaag tcttttttca gctacaatag atgaagagat aaaatatata
     4861 tgtgaaaaat atatgctaga ttacagtgtt ataaatatag aagaaaatga gagcgatact
     4921 aatcaaaaaa caagacaaat agatgacaaa ataatcaagg caaatggaag agagaagtac
     4981 atccttttaa aagaattaat atacagcgaa aatccaaaga gtgtgataat attttgtaat
     5041 accaaagaga aagtatctaa attatataat aagatgagta aagagggttt tttaataaga
     5101 gagttacatg cagatttatc acaagaaaga agaatttttg taattaagga ttttaaaaac
     5161 caaaaattta atatattagt tagtagtgat gtagcgtcca gaggaataca tattgatgat
     5221 atttctttag taataaatta tgatgtgcca caagataaag aaaactatat acatagaata
     5281 ggtagaactg ggagaaaagg aaatagtgga aaggcaatta ctatagtaac tgaaaaggat
     5341 gaaaaatata tagagaatat agaaacgtat ataggatata aagtaaatga attaaaagat
     5401 atagaaaaaa gtagaattac acatggcaaa attttgtttg aagaatattc aaaaggaatc
     5461 ctaaaaaaag tatcaaagag aaaaaaagtt gataaaaatt cttacaaaag taaagatata
     5521 aaactaaata tagaaagtga agttgttaaa ttatatttaa atgctggtaa gaagaagaaa
     5581 ataagagttt tagatatagt tggtgcattt agtaatataa aaggtataac taatgatgat
     5641 ataggtgtaa ttgaagttca agatttatgt tcatatgtag atatattaaa ttataaggga
     5701 gatttgatat taaaaaaata taaggagata cctataaaga aaaaaatggt taaagtgaaa
     5761 agagatatac gataatttag ttattactgc tctctctgat gatgaaatca tatatttaag
     5821 taagagtatc aatatttatg aattattctg ttatcgaaat gaagtttcaa aaaacaatag
     5881 aagttgttaa ggaaaggaat gttgacttta tctacattct aagctatact atttttaaat
     5941 gtttatgaca atagtcattt taaatataag taataaatat atacctatag ggatattaaa
     6001 ggaggtaata tatggcatat tttaactata acgacaaaca gtgctactat aatgaaattg
     6061 gggatggtac accattactt tttttacatg gaaatacagc ctcttccaaa atgtttaatg
     6121 atataatcga tttttataaa gatgaatata aagtaatatt aattgatttt gtaggtcatg
     6181 gaaaatctca aatggtcgat aaattttctg ctgacttatg gtttgatgag gctatgcaag
     6241 tgatatgttt tttagaagca atgaactata aaaaagtaaa cataattggt agtagtggag
     6301 gagctcttgt tgcattaaat gttgcacttg agagacctga tttagtgaat aaggtaattg
     6361 cagatagttt tgaaggagaa gttcctttag agtcatttgt acaaaatgtt aaaatagagc
     6421 gtgaggcatc aaagcaagat gatggtgcaa aagcttttta tatttataat caaggagaaa
     6481 actgggaaaa agttgtagat aatgatacag aagctatttt tgaacattat aaaactatag
     6541 gaaaattttt tcataagcct ctagaaacta tgcaaccaga ggtgctttta acaggaagta
     6601 gagaagatga gtttgtatct ctaatatcta gtgatttttt tgaaaatact ttttcattac
     6661 ttcttgaaaa gattaaaaat ggtaaaatgt atttgtttga taaaggtggt catcctgcaa
     6721 ttttatcgaa tggattagat ttttcaaatg tagcaaagaa atttttagag gaataattta
     6781 accaaaattt acaataatga aaactgtaaa atggattgca aaacttacaa aagtgagtgt
     6841 acatatactt cattattatg atgaaatagg attgttaaaa caaaatattc taatagtcaa
     6901 gttattacat ttgtatatgc aatatgtata gaaagagata tcattagatt gtaaagttgg
     6961 gattaaataa caatatgaaa ttttatttat tcagatattg tagcttaact atataaatgt
     7021 tagaataaat ttatatagtt aagctgtagg attaaataat ttagttaaat aagtctatta
     7081 tctcttcatc agatagactt tttaatacac cttcattaga aagttctcca tttataaatt
     7141 gatttataag ctctttttta ctttcttgta attttattat tttttcttct atagtccctt
     7201 tagcaatcaa ttttataact tgtactgaat ttttttgacc aaatctatga gctctatcac
     7261 tagcttgatt ttctactgat ggattccacc atgggtcaaa gtgaattacc atgtcagcag
     7321 atgtaagatt taaaccagtt ccgcctgctt ttaaagatat taaaaaaact tttttatcca
     7381 tactattatt gaactcatct acaagttcaa gtctttcttt ggcattagtt ttaccatcta
     7441 tataatggta tttaatttta tacttatcaa gttccttact tatgttctta agcacagaag
     7501 taaattgtga aaacaataat attttgtggt tttcgtttat actatcccta agtatttcta
     7561 aacatgtttc aatttttgaa ctttttttat tgtaatcttt tactactata cttgggtcta
     7621 aacaaagttg tcttagttta gttaaataag aaaatataac tattttatct ttctttagat
     7681 ttttatcttt cattttatct tgtatatctt ttgaatatac actatatatc tttttctgct
     7741 ctttattcaa ttcaacaaaa aagttttttt caatcttatc tggaagttct ttcataactt
     7801 gttttttact tcttctaaga ataaaaggtt ttattaattt tttcaggttt ttaacattat
     7861 cctctttatt tataaacaat tcctgaaact ttgctttact atatagatag ccaggcatta
     7921 taaaatcaaa tatagaccat aattctagta gattattttc cataggtgta ccagtgagtg
     7981 caaatttatt ttctgcattt atacttttta cagactcagt tactaaagct actggatttt
     8041 ttatattttg agcttcatct aatatacaat aatcaaactt tatttcacta tatttttcta
     8101 aatcatttct aagagtacca tatgttgtaa gtattacatc aaaattttct aattccataa
     8161 aacatttttc tctatctctt ttattcccat gtatcaataa aacttttata tctggagcaa
     8221 acttttcaaa ttcatttttc caattataaa tgagtgatgt cggtgtaaca ataagacttt
     8281 ttttattaga taatgataat aaaaatgcaa tagtctgtat tgttttacca agacccattt
     8341 catcagctaa tataccacca aatttataat aatctaaaac tttaaaccaa ttgaggccat
     8401 ttatttgata atcacgaagg tttgcattta aatttgcggg aatttctaaa tttatgctat
     8461 caaaatttct aaatttttca catatactgt ctagttcttt tttaccatct ataaacttta
     8521 aatttttact acgtattaca tcatttatat atagagcttt atttttataa attttattat
     8581 tttttgattt atccattata tctagatttt caactaattc aaaaaaatct tttgtttctc
     8641 tttctgataa atctataaag tttccatctt taagtttata aaaagtacgt ttttcttgaa
     8701 aagctttcaa aatatccttg tactcttttg gatttatatc agatatatta aaattaaatt
     8761 ctaggtaatt ctggttagtc tgattgattg aagcttgaat tgatgaagcg ttatatattt
     8821 ttttcaattt aaacttctca gaataatata tatctccaaa ttcctttaga gattctaatt
     8881 gtaaatctaa aaatgtaaat aggtcatagt cagttccttt aaacacataa tgattatttt
     8941 cctttgtaaa ttgtgaagaa gtaagtttat tttcaatagt agtttccttt tgtatgttgg
     9001 ggagcacaaa ttttttatcc tcatcatcat atatatactt tacatcacaa catatgttgc
     9061 catcaatcat atcaaagtag aactcagatt taaaatcttt tgttatatta ttttttatgt
     9121 tatcatctaa atgaacttct tgagatatag attttaatct aggaactagc aatcctaaaa
     9181 catccttaga atcctcttta tcaaatttta actctttagt ctttgataat atttgatata
     9241 tttttgaata atcatgagct tttttatgtg ataataaata gatattgcca tcatataaaa
     9301 gtacatcatt ttttttagta agtggaacag gtagtgaatc tttagaatat aagactattt
     9361 tatcttctat atttttaaaa tcaaacctta caggtagagt atcatttaat atctttggat
     9421 gatattcctc tttagaaaaa ataagggtaa aatccttgta cttaatagca tttaaaaatt
     9481 gttttaaaga gcttgcattt atctccataa atttttttga atgtgatgaa gaaaagttgt
     9541 atgaaagatt tgtcccatat tcttctataa tatctactaa cttctcatct tcatcagcaa
     9601 aataatgaat gttggggttg taagtaaatt tcttcccaaa atctaattca tatccattaa
     9661 gttttgaata tgcaaattca gtaatgtttt ttaaaatgta cattttatca attcctattt
     9721 taaattcagc aattgcttta tttgaatcca catttatcaa tacatctaaa ttaacccttt
     9781 ctcttttagt attgtctaac aaagaaagta attgcttgct taaatctaag cttgttttag
     9841 aatttgtatt agaaagctta tccttatctg cacctaatat atctattaga ataggaggaa
     9901 catctctttt attttttatt tgttttaagc tttttatcac aatagcaact atatgcttac
     9961 atataaaaat acttttgccc tttgattgac agtcagcgca actacaatca ttttcaatta
    10021 tcttaaagtg ctttatatct atagttattg aattaaaata aaagctataa ttatcttcag
    10081 aattgactcc accacgaaat gtcaacaggt tattttgtag gtcatcaata tctatgtcta
    10141 tataatcaac taattgatta gtataatatt ttacacctct tgaccatctt gatggttctg
    10201 tatttcttct tattagttta tatatcatat ctattttcat attattcacc ttacctcact
    10261 gtatatatta taatctaaat gcattcaatt tacactctaa atttaagact aaaattaagg
    10321 cagatatgaa tagcttttat ctattacaat atatgtgtaa ataacaattt actattagat
    10381 ttttattgaa ttatcttata aact
//
LOCUS       DAEPOG010000134        10375 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEPOG010000134 DAEPOG010000000
VERSION     DAEPOG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.134"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            202..318
                     /locus_tag="KR432_002773"
     CDS             202..318
                     /locus_tag="KR432_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBH0641293.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            482..1549
                     /locus_tag="KR432_002774"
     CDS             482..1549
                     /locus_tag="KR432_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0641294.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1741..2271
                     /gene="rnmV"
                     /locus_tag="KR432_002775"
     CDS             1741..2271
                     /gene="rnmV"
                     /locus_tag="KR432_002775"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBH0641295.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2442..3311
                     /gene="rsmA"
                     /locus_tag="KR432_002776"
     CDS             2442..3311
                     /gene="rsmA"
                     /locus_tag="KR432_002776"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH0641296.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3377..4648
                     /locus_tag="KR432_002777"
     CDS             3377..4648
                     /locus_tag="KR432_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641297.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4799..5914
                     /locus_tag="KR432_002778"
     CDS             4799..5914
                     /locus_tag="KR432_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0641298.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6100..6996
                     /locus_tag="KR432_002779"
     CDS             6100..6996
                     /locus_tag="KR432_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0641299.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7066..7863
                     /locus_tag="KR432_002780"
     CDS             7066..7863
                     /locus_tag="KR432_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641300.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7919..9271)
                     /locus_tag="KR432_002781"
     CDS             complement(7919..9271)
                     /locus_tag="KR432_002781"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH0641301.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9490..10368
                     /gene="purR"
                     /locus_tag="KR432_002782"
     CDS             9490..10368
                     /gene="purR"
                     /locus_tag="KR432_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBH0641302.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
BASE COUNT         4193 a         1125 c         1877 g         3180 t
ORIGIN      
        1 aatatttagc gattattaaa aagatatata tatatatttt aatttgagca gtttgttata
       61 tattataaaa aaagagaaaa ataaaaggta aattgtttct ggataactta aaattggaag
      121 taagaatatt taactagaga agaattattt aagttcaggt tagctctagg agtagctgat
      181 tttataggag caatagattt tatggatgat gatataaaca tgatagcaat tagaccagaa
      241 gatataaggg tagaatttaa tagagatttc aaagaaatta tagacctcaa actcagaccg
      301 tatcgatata aaaactaaag ctagatgata tagtgttgta tataggacca tcagctgtta
      361 tggaagtatt agtgctttta acaaatataa tagtaaaact tttgtatgaa ttgataataa
      421 aaaaattaaa aaccaagaca taaaaatggc aacaaaaaga agttgaataa gggaggtaaa
      481 aatgaaattt aagaagttag tctctttggg aatattatca acacttttat tgagtgtaat
      541 aactggatgt agtaatgttt catcaggttc aaacttaaag tctaataata agacaaaagg
      601 agagttaaca gtttatacag caattgagga agatagtata gatacatact tagctacatt
      661 taaagagaaa tatccagata ttaaattgaa tgtacttaga ttatcagtag gaggtataac
      721 agcaagatta ttagcagaaa aagataatcc taaagcagat gtgttatggg gtgttgcagc
      781 tacaagtttg ataattttag atgacaaaga aatgctagag ccatacgctc cgaaaggctt
      841 tgataaggta aatcctgcat ttaaagatga taagaaagtt ccttcttggg ttggaataga
      901 tgcttggatg actggtataa cagtaaatac taaagaatta aaagctaaaa acatatcagc
      961 tcctaattct tatgaagatt taataaagcc agaatataaa gatcttatat ctatgcctag
     1021 tccagcttct tcaggaacag gatatttaac agtatcagaa ctcatacaaa ttatgggtga
     1081 agaaaaagca tggcagtata tggataaatt gcataaaaat attggtgtat acactcagtc
     1141 tggttcagcg ccagctatat tagcagcttc aggagaatat cccattggta tatcttttgg
     1201 aaatagaggt ataaagttaa aaggtgaagg ttatccagta gaagtagtat ttccaaaaga
     1261 aggttctgga tgggagatag aatcaaatgc acttgtgaaa aaagataata taaaagaagc
     1321 atcaaaagta tttttagatt gggctataag taaagatgca atgaatgagt attctaagaa
     1381 ttttgcagtt actacaattg acacgggaaa tccggtgcca gaaggatttc caaagaatcc
     1441 attagaacag atgatagata atgatttaaa atcagcagca agaaatagag aggctatact
     1501 aaataaatgg atagaaaaat atgatggaaa gacagaaaaa gagagctaag ccttacaata
     1561 aaatttagta caaaaagcgc ctagaatata agttttacat ctaggctctt ttgatatatt
     1621 tatttaaaat gcatatttct atataaaaat aaatagttta gtaccttgat taaattaaat
     1681 aaaaacctta aaataaagag atgtaaagaa aaactaaaaa cgaataaaag gtgaatttat
     1741 atgataaaag aaataatagt agtagagggt agagatgacg ttacagcagt taagagagca
     1801 atagatgcag aacttataac tacaggtgga tttggatttc caaaaggtgt aatgcagcgt
     1861 ataaaagctg ctaatgaaag acgtggtgtt ataatattta cagacccaga ttttgcaggt
     1921 gaaaaaataa gaaaaaaaat agcatcagaa gttccaggat gtaaacatgc atttctacca
     1981 agagaggaag ctaaaaagga tggagatata ggtattgaga atgctactcc tcaaagtata
     2041 atagcagctc tgaataaagt tagaactgaa aatacagaaa aaagagatga atttaagcaa
     2101 gttgacttaa ttagaaatgg tcttataggt aatgaagaag catcacttag aagagatgca
     2161 cttggaaaaa tacttggaat tggatatgga aatgcaaaac aatttttaaa tagattgaat
     2221 aattatggag ttgaaagaga agaatttata gatgcattaa aacaaatcta aacgtaatgt
     2281 ttaatgtatg aaaaatatgt atataaaata aattgtggaa ttacggttaa tttaagataa
     2341 attatttata atatatacct tattttaaat caatgtaaat acatataaat aagtataatt
     2401 aataaaatta aatatcaaaa taagttaaaa aggagtaaat aatggataga ctatcttcac
     2461 ataatgcaac aaaggaagta gtacaaaaat ataattttaa attttcaaag tctttaggtc
     2521 aaaatttctt gatagatagt aatataattg ataaaatctt atctggagct agaatcacaa
     2581 gaggagataa tatcatagaa gtaggacctg gtataggaac tcttactcgt gagatgggta
     2641 aaatagcaga aaaagtagtg gctatagaaa tcgatagaaa tttaattcct atattgaaag
     2701 ataccttgtc agatttagat aatacagagg ttgtaaatca agatatacta aaagtagaca
     2761 tacaggaatt agtaaaagat aaattaaatg gtggaccagt taagcttgta gcaaatcttc
     2821 catattatat aactactcca atagttatga aatttttaga ggaagacata cctgtaacag
     2881 acatagtagt catggttcaa aaagaagttg cagatagaat gaatgctatt ccgggtacta
     2941 aagattatgg ggcattatct atagctgttc aatactattg tgatacagag attgtggcaa
     3001 aagctccaag acatatgttt ataccacaac caaatgtaga ttccacagtt ataggtttac
     3061 atgtaagaga taaaagaaaa tatgatgttc ataatgaaga catatttttt aaaacagtaa
     3121 aagcatcctt tggacaaaga agaaaaactc ttcttaattc gttgggaggt cttggttttc
     3181 ttaataaaga tgaaataaga gaaatattaa aagaagctaa tatagacgaa aaaagaaggg
     3241 gtgaaactct tagtatagaa gagttttcag ttctttcaaa tataataaat acaaaagtat
     3301 cttctaaata gaaggtactt tttttaattt tgttaacata tattgtttag agaaactgtg
     3361 ttaagggggg attgcagtgg gttctaaaag aaagtttaaa agagactata taatactaga
     3421 agctaaagac ataaacttta gatacaaaga acgagtactg ccaaaagctt ttgcaaaagt
     3481 tgaagtaaat gatgaaaaag caattattgc attatatgta gaaaatctaa aatatgttaa
     3541 agatgggtat aaagcagtag caataaaaga tgaccatgga ttaatagact taggaaatat
     3601 aattttaaat gaacaaggaa gaggtgaatt tgttttagat ttagaagatt ctgatgatga
     3661 gataaaaggt attgcattat tgtatgaaag aaatatacct ttaattggat ttaagggaaa
     3721 taagcttgaa aattatgagg acatattatt tcaagaggta gaggaagaag aatatgaaga
     3781 atatgaagaa tatgaagagt tggaagaaga cgaagagata gaggataatg aggaaagtga
     3841 tagagacgaa gatagtgaag aagaatatat tatagaagaa tatgatgaag atgagtatga
     3901 agactattgg gaagatgatg aagatattga agaaggagag ttaaaagcaa gaacggaagt
     3961 agaagattct ggtgatgagg aaatagaaga gttcaataat tataaggaaa tagaaaatta
     4021 taatagggtt aaagtacagg aagaatatat agacatagaa tatgatgagg aagaagagga
     4081 agaagaagaa gtttttgaag taaatagaga agaagatagg tttgtttcct caaaacctac
     4141 taaatcaaag aaagttcaaa aagatcagaa taatattgaa aaaataaaaa gagctaatgg
     4201 agtaagtcaa ggagagagtg gaaataaaca aaccagtaca ttacttatgc caagacaaat
     4261 aaaaaaaggt ttgaaattat ttaaagaagt aaaacctttt gtaacagatt atatagataa
     4321 gacaagatgg tggaaaatag agataaatcc tacaacactg tgtggatata caatgcctta
     4381 tttaggatat ttaaacgtat tgaattacac tatgtatagt gatgcagttt tacattcata
     4441 taaatacaga cactatttat ttggtgttca gtatgatgaa tataataaga gaaaacatta
     4501 tatttatgct atcccaggaa atagaaatga acagccagac aatggaagca caggattttt
     4561 aagatatcaa gcttgtgata atagaacaaa tagtttagga tattggctat gttttataga
     4621 ttctagacaa agaaatatag ttaggtaaaa ataataaaaa tgaaaaaatt tagactcaat
     4681 tcttatttta ctgaaaaaat taatgaaatg tagtacaaaa ctttaaaaac tattgataat
     4741 atttaaatat tacactacaa tatatcttga aatacctatt atattatgga ggaaaattat
     4801 gataaacgag caaagaattt taaatgagtt tcttgaatta gtacagatag atagtttatc
     4861 tttaaaagag gggaatgttg ctaaagtatt agttaaaaaa ttagaagaaa taggttgttc
     4921 agtagtaata gacaatgctg gtgaaaaagc aaatggagaa actggtaata taatagctac
     4981 attaaaagga aataaagaag gaaagaaaat attatttagt tcacatatgg atacagtaac
     5041 tccaggtata ggggtaaaac ctatagtgga tgaagcaaat ggaataataa aaagtgatgg
     5101 tactacaata ttaggttctg atgataaagc aggtatagca gctatattag aaggcttaag
     5161 atatataaat gaaaataaca tagaacatac tgatatacaa gttgtattct ctatatgtga
     5221 ggaatgtgga ttagttggag ctaaaaattt agactatgga aaaatagatt ctgaatatgc
     5281 atttatatta gatagtggtg gttctccagg tgaaataata gtaaaagctc ctgcacaaga
     5341 tgtaataaat gttaagatat taggaaagac tgctcatgca ggtcttgaac ctgaagctgg
     5401 aataagtgct ataatggtag ctgccagagc aatagaaaat atgaatttac ttagaataga
     5461 cgaagaaaca actgctaaca taggaattat aaacggtgga acagctacaa atatagtaac
     5521 aggagaagtt aatatagttg cagaagccag aagtctaaaa gaaaataaat tagatgttca
     5581 aactaaacac atggtagaga cttttgaaaa agctgcaaaa gattttggag ctcaaattga
     5641 gatagatgtc aatagagcat atacaccaat agatgttagt gaggattctg aaataataaa
     5701 acttgcaaag aaggcttttt caaacttagg tatagaaggt catactgaat ctacaggagg
     5761 aggtagcgat acaaatatat taagtaagaa tggtatcgaa gctataactt taggaattgg
     5821 tatgaaaaat gctcatacac taagtgaaca tatagctata aaagacttat atgactctgc
     5881 taaaatggta gtggagataa taaaggaagc ataaatttaa ataatagtgt ataatatata
     5941 tatcgcctag cttaagctag gtgatttttc attttataga agattgataa tctctattga
     6001 aagcaaagag tatacatttg catataagag agtgtttaat ttatgttcag atgtaggttc
     6061 ttaataatat ttagattgtt tcataaggga ggaataaata tggaaacaag gtatgaagaa
     6121 gcaaataaaa taacaataca atcaatactt tggaatgtag tattaacaat aattaaggtt
     6181 atagctggtg ttattggtaa ttctagcgct atgattgcag atggattaca ttcagcatca
     6241 gatattataa gttctatagg agtactaata ggcaattatg tatcttctag acctggagat
     6301 agagagcaca attatggtca tgagaaagca gagacattag tatcatttgt gttatcaata
     6361 ttgcttattt tcgtttctat aacaataggt atagaggcaa ttaaatcttt atttaactta
     6421 gatgcattaa gtgttccatc aatattacca ttagtagtat cagttatttc aatcctgata
     6481 aaagagtatc aatatagaat aacaataaaa gttgctaaaa agataaattc accagcctta
     6541 aaagctgatg cttggcatca taggtcagat gctttatctt cagtagctgc ttttataggt
     6601 ataggtggtt ctattttagg gtttaagcca ctagacccaa ttgcgtcaat tgtagtggct
     6661 atatttgtag ctaaagtagg aattagtata cttataagtt ctgttaatga attgatggat
     6721 gtttcagttg acgaagaaga gataaaagaa ttaaaattta ttgtagcaga tacagaaggt
     6781 gttaaaaatc ttggtgatat aaagactaga aaacatggag ctatggcata tgtagatttg
     6841 actatatgtg tggatgaaaa tttgacagtt aagcaaggtc atgatattgc taccaagctt
     6901 gaaaaacata taataaaaca tatggaattt gtaaaaggca taacagttca cgttgaacca
     6961 tgtactaatt gccaaggaaa taagtgtaat aattaattaa aacttaaaaa aaagtgtaaa
     7021 aagttatata attgttttat gattataaac tctaggggtg gttaaatgag caagaaaaga
     7081 aaactaaaga aaaaagctgc aatattaata ggagtagttg ttttctttat aatatacttt
     7141 atatccttta gaataggaat aaatatggaa aaggataaaa gtgaagtgcc aaagaaggta
     7201 gaaaaagaag aagaagataa taggtcttta aggactcaaa taaaagaaaa gaaaaaggtt
     7261 tatatatcag acaaaaatat agaaaacata aaaattgaag aagatatgtg ggatgaaata
     7321 aagttatttt tctcagattt taagaaagta agaaatacta cttcttctta cgaagcagtt
     7381 tatgaaggtt attctgatga tggagttaaa ttttctactg accttaactt ttttagagta
     7441 tatactgtaa ataaagaaga atattataaa gtaccagtta gttctaagga tgcttttaag
     7501 aaacttttag atgagagtat atatacttca tttgaccttt taagtcatta taaagattgg
     7561 aaagaggtta ctataactca tggaaaagaa attaaaaaag ttcatagatg gaaatttgat
     7621 gatttagcac ataagatgat ttcaaagagg gttgtaggaa aagttcaacc tgagaaaagt
     7681 aaggaaagaa gtgactataa ttttactata aatataaaag gtgataattt tgatgtaaaa
     7741 gttgatacaa tgggaaaaga ttatgtaaaa gttacttcta agaaaaatgt atcttactat
     7801 gaggttcata caagtttatt tgattacctt aaaaatgaga tatttgaaat tggaaagaaa
     7861 taagacttta agaaggctat caaattagat atttgatagc ctaaattatt tttatatatt
     7921 atttttcaac caacaagtct gcaaccttat aaattggacc agcaccagca gttattacta
     7981 aatcattatc cttaacattt tcacgtaagt attttactat atcttcaaat tcttttatat
     8041 agatggcatc tacattgttt tgatataatt tctcaaccaa gtccttagaa tgtatatctc
     8101 cagggtcttt ttccctagca gcatatatat ctgttatgat aactttatca gcagcataga
     8161 atgcctctga aaattcacct aaaagagatt ttgttctagt ataagtatgt ggctggaaga
     8221 tacaccataa agttgatttt tttaattttt tggcagcagc taaagttgct tttaactcag
     8281 ttggatgatg tgcataatca tctactacta aagcattctt ataataccct ttagtttcaa
     8341 atcttcttcc gacaccttta taaattttta tattttttct tatagtctct aagtcaatac
     8401 cagaaacaag agctgccatt atagcagaag aagcattgta tatgttatga agaccatata
     8461 cagaaagttc aaattctcct aaatctcttc cttcatattc tattctaaat attccatgac
     8521 cactattgtc aaagtgaata tcttttatta cagcatcatt gtcagaatct ctaccgtatt
     8581 ttataatagt agctttaaca tcatacaata tgtcatcaat attttcatca tcgccattta
     8641 ttatgaaata gccttctggg ggtaataatt ttccaaattt gttaaaagaa gccttgattt
     8701 catctattcc agagaaataa tcaagatggt cttcttcaac atttaaaact atagatatct
     8761 ttgggttaaa attaaggaaa ctatctacat attcacatgc ttcagttata aagtggttgg
     8821 aattacctat cttaacatta ccacctatca tacttaagtt accaccaact agtatagttg
     8881 ggtctaagtc tgcatattca aagattgtag ataacataga tgttgtagaa gtttttccat
     8941 gtgttccaga aactgctata gaatttttat attccctcat gatttggcct aaaaaagtcg
     9001 ctctgttcat taccaattta tttttttctt tagcagccac aagttcttca ttgtctggat
     9061 gaactgctgc tgtataaaca accatattta catcatctga tatattatct tttttctgac
     9121 caatataaat agttgcccct tggtccctaa gtttgtctaa taggtatgat tcgtttgaat
     9181 ccgaacctga aacttgataa cctttattga tacatatttc agctaatgca ctcatgctga
     9241 taccgccaat tcctataaag tgtatattca taataaaacc acctttcttt ttaattaagt
     9301 cctacaatat gaactattta tgtggtataa taatataatg ttcaatattt tataattaat
     9361 atgcatataa tattatatgt attttatttt tgaatataat aaaacataag taatattata
     9421 ccacaataag aattgttatg cattagttag tttaacgtta tataaaggta cttgtaggag
     9481 gaaataataa tgaaatttaa aagaacagag agaattggag caatagttaa aatactgtct
     9541 gataatccaa ataaaatata tacattaagt tattttacaa atcaatttaa tgcagcaaaa
     9601 tcaacaataa gtgaagattt attagttgta aaaaatgtat ttgagaagct acatttaggt
     9661 aaagttataa ctatctcagg agcagcagga ggagtaaaat atatacctaa aacttctata
     9721 gcagaaaatg cagagttctt aatggaatta tgtgagagaa tatgtgacaa gtctagaata
     9781 ctatctggtg gttttttata cttgattgac ctaatttatg accctacaat agctgctaag
     9841 ataggaaaga tattcgcatc aaatattgag tatgtagatg cagattatgt tgtgacaatg
     9901 gaaacaaaag gtatacctat ggcacttatg actgcaaaag ctatgaattt acctctagtt
     9961 ataataagaa aagatataaa ggtatcagaa ggtcctacac ttagcatgac atatgtgagt
    10021 ggaaacagct ctaaagttga aagtatgagt ttgccaagaa aagctcttaa accagatagt
    10081 aaagttataa ttatagatga tttcatgagg ggtggaggta ctataaaagg aatggtagac
    10141 cttatgaatg aatttggtgc agaggttata ggtactggag tattcatatc tacaacgaat
    10201 ccttcagaaa aaatggtaaa agattatatt tcattgattc aattggatgt taatggagat
    10261 aaaatagtag tagaaccaaa cttaaagact tttaaagatg aatatagaaa tgaagattta
    10321 gacgaagaag aagattcaga atttgaattt gagatagatg aagattaata aaaaa
//
LOCUS       DAEPOG010000135        10359 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEPOG010000135 DAEPOG010000000
VERSION     DAEPOG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.135"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            92..1846
                     /locus_tag="KR432_002783"
     CDS             92..1846
                     /locus_tag="KR432_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBH0641303.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            2230..3216
                     /locus_tag="KR432_002784"
     CDS             2230..3216
                     /locus_tag="KR432_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0641304.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            3439..3522
                     /locus_tag="KR432_002785"
                     /pseudo
     CDS             3439..3522
                     /locus_tag="KR432_002785"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            3553..5034
                     /locus_tag="KR432_002786"
     CDS             3553..5034
                     /locus_tag="KR432_002786"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH0641305.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            5060..6031
                     /locus_tag="KR432_002787"
     CDS             5060..6031
                     /locus_tag="KR432_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBH0641306.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(6287..6889)
                     /locus_tag="KR432_002788"
     CDS             complement(6287..6889)
                     /locus_tag="KR432_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0641307.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            7170..8381
                     /locus_tag="KR432_002789"
     CDS             7170..8381
                     /locus_tag="KR432_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBH0641308.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            8449..8646
                     /locus_tag="KR432_002790"
     CDS             8449..8646
                     /locus_tag="KR432_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH0641309.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            8680..9651
                     /locus_tag="KR432_002791"
     CDS             8680..9651
                     /locus_tag="KR432_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0641310.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            9838..10215
                     /locus_tag="KR432_002792"
     CDS             9838..10215
                     /locus_tag="KR432_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641311.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         4242 a         1038 c         1704 g         3375 t
ORIGIN      
        1 aatatatacg ttactataat attatgttat tatttgtaaa taaggggata atttataagc
       61 aaattaaaga ccttaattta caggaggaat agtgtatatt atgaataaaa aattcaaaaa
      121 attcagtaaa aaactaagta tattaatttt agttttgact atgtttcttt ttaataattt
      181 aactgcaaat gcacagatta aagaagattc aataatagga attgatagat atgaaacagc
      241 aggacttata gcagatagac aaacttatga tactgtaata ttagttaatg gagataaaaa
      301 tttatctgat gggctttcat caagtggatt agctggagct attaacgcac caatactttt
      361 gactaaaaaa agtgaaatac caaaagctac tttaaataga ctagataata agagtttaaa
      421 tgttgtaaaa aaagtatata taattggtgg ttataatact attgaaaatt cagtagaaaa
      481 agatataaaa agaaaaggta ttcaagtgga aagaataaat ggtaacaata gaattgaaac
      541 tagttataat gtagctaaaa aaataaatga agtaagtagt gtaaaagagg taatgcttac
      601 aaatggattt gtaggagagg ctgatgctat gagtatagca cctgtagttg caaaaaataa
      661 aggagctata atacttacag atgggaaatc agttccattt aagactgaag gtttaaatgt
      721 ttacgctata ggtggcaaat ctgcgataaa tgaggattta gtaaatgaaa ctaaagcaac
      781 tagaatcgga ggaaatgata gatttgagac caataaaaag gtaatggaaa agttttataa
      841 tggagcgaca gatttttata taacaaatgg atatcaatta gtagatgcac taacattatc
      901 tcctttagcc aaagaaaaac caatcgtttt agttgctgat ggaagtaata aaggtatttt
      961 aaaaggagct aaaagtataa caaaagttgg aggtattgat gccaatatat ataaacagtg
     1021 tttagatatg attaaatata ataatatgga tgtgactcca aatattttaa aacacttaac
     1081 ttcagttgaa ggaaatgaag tttctgaaat acttatagaa tcagataatt taggtgtggt
     1141 tgttgaaaag acgaatacca ataaatttga gtttgattac gtaagtgtaa ctaatgaaaa
     1201 aaattgtact ttttctgtat ataaagaaaa ttcttctaat aatgtaaaaa atggaaagct
     1261 agttgtaagt gctaagaaaa aaatagataa gaaagatagt gatttgcaag atatgaatgg
     1321 cgacaatatg ataaatgcaa ataaagatag gatggtaaat gtgataaaaa ttggagttcc
     1381 cgataaaatg tattctaatt ttaaagtaga tgttgaaagt ggttcagtag aactttataa
     1441 tattaaagga ggagctactg ttgatgtcaa tgacggaatt gctaacattg ttgataacaa
     1501 tgtgacaaat cctttcaata taaatactaa tagtgggatt tcaggtgtaa cagctgaaac
     1561 tatatcatct gagataaaat ttagagcaaa caatggaatt gtaagtgtaa cagctacaaa
     1621 tataaatggc aatataagtt tatttggaag cgataataaa ggtacctttg atggaatatt
     1681 taaattaaat ctgaaaaaag aaccatcaaa tttacacttg aagttagttg gaaatgggat
     1741 aaataaattg ccagaaggtt ggtctaaaga ttatatttta ggaaatggac aaccaattat
     1801 tgaagttaaa aataatggca taaataatat atcaattgga gaataaaaac atatgaaaat
     1861 aaagtttata tagttttaaa aattatagaa ttttaaaata tttatatata ttctttaaat
     1921 aaaaaattat tttttaagtg ataattaagt atttatcgat aaggctatgg ttgtaaaaat
     1981 catagcctta tttttatatt ataaataaat tagagtattt atcaattcat agtagataaa
     2041 attgaaatta aatataacaa caaaaaatga aaattacaat taatttaatg ttaaagactc
     2101 aattatttgg atgtggaatg gatagtacaa gtagtatata aaaagtattg actggacaaa
     2161 taactatatt ttattttaaa tatataatat aatgtaataa aatatatcaa atgtagaagg
     2221 tgataaccta tgaaaaaaat aacaataaat gatattgcta acttggcagg agtatctaaa
     2281 agtactgtat caagatatct aaataataaa gatatcagtg attctactaa agagaaaata
     2341 aagacaataa tagatgaata tggatatgag ccgaatgcat ttgctcaaag tttgagagct
     2401 aaaaaaacat actttattgg tattataaca ccgtgtttag attcttttgt aaaatctaaa
     2461 attatgatgg caatagatga agaactcaaa gaattaaaat atacatcact aattattaat
     2521 actaatagaa aaataaggtc agaaatagat agtatatcaa agttagctag tctaaaagtt
     2581 gatggaataa tactaattgg taccgaaata actaaagaac ataagaatga aattgaaaag
     2641 ttagatatac caatggttgt agttggacaa aaagttgatg gtatcaatag catagtgaat
     2701 gatgattatg gagcaggata taaaatgggt caatatattg ctaataaagg ttacaaaaac
     2761 attgtatatt taggtgttga tgaaagtgat atatcagttg gtttaaatag aaaaaatgga
     2821 gtacttaatg ggttaaaaga taaagggtat gatgcaaaag tattttatac tgactttgac
     2881 caagaaacgt ctatacaaag aagtggagaa atgctagaaa gtgaaaatcc agatataata
     2941 atatgtgcaa ctgataatat agcaatagca acaatgaaag aaatcaataa gagaggtaaa
     3001 aatataccac aagatatatc tgtagctgga tttgggggat atgatgtatt gtctattata
     3061 agccctaaac ttacaacaat aaagtttgaa aataaaaatg caggtaaagt agcagcaaat
     3121 acgatagtga acttaataca agaaagaaaa gaaccactat taaaggagat taaatttgaa
     3181 ttaatagaag gtgaaagtac aataaataaa aattaagata cacattacat atattagtaa
     3241 tgtgtatttt tttactaaaa aaactataat tctaaaaatt aggaaaactt ttttgaaaga
     3301 aatattgcat ctgaaataaa tttatgctaa tctttaatta aaccggtttc gaaaccggtt
     3361 taattaaacc gaaagaatag cacaatgagt gcaaaaataa ctgattgtaa caaagtatca
     3421 aagtaattat tagagtacat aggaaataaa caaaatataa aaggtgtagc acattgtgta
     3481 acaagattaa gaattgtttt agatagtaat aataaagcaa atataaaatt acaaaagaaa
     3541 aggaataaaa atatgtacaa agagcctaaa tatagaacca tattagaatc gataaaagaa
     3601 gaattaaata aattaagaaa tatatcttta aatgataaat ataaaccact atttcatata
     3661 catccacaac atggactact taatgatcca aatggattag catattataa tggtaagtac
     3721 catgtatttt atcaatggta tccatatgat gcaactcatg gaatgaagca ttgggcttat
     3781 gtaagttctg atgattttgt aaactggaat agagaagatg tagcattaat accaatcgaa
     3841 agttatgaat ctcatggagc atattcaggt aatgcgatag aggtagatgg aaaattgcat
     3901 atgtattata cagggaatat aaaatatagt gcagaagata gatatgcata tcaaaactta
     3961 gctataatga acaaggatgg taaaataact aaatatgaaa ataatccaat agtaagtgaa
     4021 ataccaaaag gatatacagg acatgtaaga gacccaaaag tatttaaaag aaaggataaa
     4081 tactttatgt tacttggagc acaaacaagt gataaaaaag gagttataat agtatatgaa
     4141 tctaaaaact ctatagattg gaattttaaa ggagaattaa atgtaaaaaa tatagatgaa
     4201 gattttggtt acatgtggga atgtccagat tatattaata tagatgaaaa agatatatta
     4261 atattttcgc cacaaggagt agaacctaaa ggatttgatt atcaaaatat atataatgta
     4321 gtatatgcta ttggtaatat ggatttagat aatttaacat ttgaaataga tacaatgaaa
     4381 gaactggaaa aaggatttga tttctatgct ccacaaacat ttataaagga ctcacaaata
     4441 atactatttg catgggctgg aatgggagaa gttttatacc ctacagataa aaacaaatgg
     4501 gcacattgtc taactgttcc aagaaaatta aatataaaaa ataacaagct attgcaaatg
     4561 cctgtagatg aattaattaa attaagatat gatgaaacaa gtgggcaaaa tactataaag
     4621 aataatatca atattataga aaatgatgaa aatgtatatg agcttaatat taatataaaa
     4681 aatattgaca gcaataaatt tgggctggaa ttattttcat cacaagatga aggtgtaaaa
     4741 ttagaattta acaaattagg aaacattgta actttagaca gaagcaactt taaaaaagtt
     4801 ttcggtgttg aatatggaac aaatagaaaa gaatatataa atattgatga aaatactaat
     4861 atcaaagtac tagcggatag aagtgtttta gaaattttta taaatgatgg agaagtagta
     4921 tttactagta gaatatttgc taaagaaaat tctaatcaaa taagggttta tagtgataaa
     4981 atagtagagt atgagtatac taaatttaaa ttaaagcaag gtattgaatt ataaaaacac
     5041 attataaggg gtagaaaaga tgaaaaaagt aattagtata ggagaagctt taatagactt
     5101 tatacctaat caaaatggtt gtaaattaaa aaatgtatca gaatttagaa gagtagcagg
     5161 aggagctcca gcaaatgtta gtgttgttgt agcaaagctt ggaggaaaat caagttttat
     5221 atctaagtta ggaaaagatg catttggtga ttatataata gatgtattaa atgaagtaaa
     5281 tgtaaatact gattatgtat tgagaacaag taaagcaaat acaggacttg cttttgtatc
     5341 attaaaagaa gatggtaatc gtgatttctc attttataga aatccaagtg cagatatgct
     5401 tttagaagct gatgaggtta aaaaagagtg gtttaataat tgtcatatct tacatttttg
     5461 ttcagtagat ttaatagata gtccaatgaa attagcacat aaaaaagcaa tagaatacgc
     5521 acttgaaagt aatagtataa taagttttga ccctaatata agattacctt tatgggatag
     5581 tgagcaatct tgtaaaaagg caatatcaga atttttacct tttgctcata ttgttaagat
     5641 atctgatgaa gaattagaat ttatgacagg agaaaatgat atagaaaaat cattacataa
     5701 actctttgtg gggaatgttg aactagtatt atatacaaaa ggaaaagatg gagtagatgc
     5761 atacactaaa aaagtaaaag gtatgtgtaa aggggttaaa gtaaatgcaa tagatacaac
     5821 cggagcagga gattcttata taggttcatt tttatacacc ttattgtata aacaaataac
     5881 attgaatgat ataaaagaaa tgaaacaaga aacgttaaat gagtatttag aattttctaa
     5941 ttattatgca gctcgtagta caactattaa aggtgcgata agttcatatg caacaaagga
     6001 agagataact gaatttataa aaaatttata gtaggaatga gtaataggtt ttgaaggaag
     6061 taacatgtaa agagttttta aggatgttta ctatgaaaat aattaacata taaaaagcaa
     6121 taaagaggct gtctcaaaat agatataagc ctctttatca ttgttgaaat gataaaaaat
     6181 attttaatta ttgttacttt ttagaagaat atatatctta ttttaaataa gcaaattatg
     6241 taagatttat ttatattatc aatactttac ttggagtatt atgtctttag cagttatcta
     6301 cttcaattga attatctaaa atataataaa tttgttttgc aaattgacga gaatgttttt
     6361 ctttttcttt attcataaaa ttaggtttac ctaaatcacg ttcaacaagc attcctatat
     6421 attttaactg tgaatgctta cagtagcgct tcataccatt ttcccaaata tcagcaccct
     6481 tttcaggtct atatccacat gttgtaataa gtgctactgg ttttcctgcc catagagcgg
     6541 ggcctttctc attaccatag tacttattca ttccatacac caatctgtca agtagtgctt
     6601 tcattggaga agtgcagtac caagaataaa ttggagtagc aaaaacaatt aaatcacaca
     6661 taagaatttt cttgaaaatt ttctgaacat catctttaca ataacatcca aaatcaaata
     6721 agttatcctg acatttttta caagcaatac atggctctat tttacaatca tataaccata
     6781 ttacttcaat atttgaatta taattttcta attcctcaat aaaaggtttt aaaatagcag
     6841 cagtattacc ttttttctta ggacttccca taagaatgca tatttccata ttgatacctc
     6901 cacagtattt taaaatagtt tttagtgctt aatatagttt taggatacta tgagaatacg
     6961 ttaacaacaa tacactttgt atatttttaa taaaattgaa acaaattgtt ttatcaataa
     7021 aagttggaat gtatctataa ttaaaaatat tttaggtatg tttttacata tatgtgtgta
     7081 gttaactatg gtaatttatt gtaaaattat cttataggat tataattgct taatttatga
     7141 ttatatttaa aatcttggag gtaaaaaata tgtactcaaa tgaagactat aaatttatag
     7201 aagaaaatgc accatgttat atatatgatg agactaatat aattgaaaaa tgtaaggaat
     7261 tgaaaaataa tattttaggt tttgattttt tatattctat aaaaacaaat ccatataaca
     7321 aaattgtaaa aagcattgca gagcaaggtt ttggagcaga tgcttcttca actaatgaag
     7381 ttatgatttc atcagaatgt ggagtttcta aatatgatat ttattattca tcaccaggca
     7441 aaacagagat tgatatagaa actgttttag ataaatgcat aattattgca gatagtatta
     7501 cagaaataga acttataaat aagatatcta aaaagcataa tcagaaagtt aaaataggaa
     7561 taagaataaa tcctgatttt actatggatg atgatagtgc taaatcaagc aaatttggta
     7621 tagatataga agatatgagc agtgttttta agatgttaag tatgtgtgat tatgtatctg
     7681 tagttgggat tcatatacat attaaatctc aggtattgga ttttgaaaag ttaggacatt
     7741 attatgaaaa atgttttttt atagctttaa atttaaataa aagtaagtat attaatattg
     7801 aatttataaa ttttggtagt ggacttggag tagcttatga taatcttaga gaaaatccac
     7861 ttaatcttaa agagctttca aaattaactc aaaagattgt ttttactaat tcaaacacac
     7921 tgaaatcaag acttcttatt gaatcaggac gtttcatagt ttgtaaatca ggtaattatt
     7981 atgttaaaat tgtagatatt aaagtatcaa gagggaaaaa atatcttatt gtaaaaaatg
     8041 gacttaatgg atttatgaga cctattattt ctaatttaat tgaaaatatt acttgtggag
     8101 tagaattaga aggtcaagag ccattatata catcaaatca tgccttttct ctttcagtat
     8161 taaataattt aaaagaagaa gagtatgtaa atgttgttgg gaatctctgt acatcattag
     8221 atgtaattgc aaataatgtt aaattgaaaa aagcaaatat aggagatttg atttcaatta
     8281 gtaatgctgg aagttactcc tattctttat caccactatt attttcgagt tatgaatttc
     8341 ctaaacaata ttataaaaga aaagatggat atattgagta aacaatagga taaattaata
     8401 aacttagtat tattgaaaaa atttaaaatg cagggggtgt aattctatat ggaattgtgg
     8461 aaaagaaatt tatttgtatg ttggataggt atgtttttta gttctatagg gatgagccaa
     8521 atagcaccta tacttccatt atatattaaa cagttggggg taactgatgt tagtttaata
     8581 caacagtatt caggtataat ttttggatgt acctttgtag tagcagcatt cttttcgcca
     8641 atttgaggga aagctgctga taaatatgga agaaaatcaa tgcttttacg tgcaagtatt
     8701 gggatgggaa tagttatatt taccatggcg tttgttcaaa atgtttatca gttattagga
     8761 ttaagaatac ttcaaggagt ttttacgggt tatgctactg catgtactac tcttattgca
     8821 acacagacag ataaaaatca ttcaggatgg gctttgggta ctcttgcaac tgcatcaact
     8881 actggctcat taattggtcc aacagtagga ggatatatag aatcaatctt gggtcttaaa
     8941 agtacattta ttataacagg aggattgttg tttgtatcat ttatcatctc tattttattt
     9001 gtagtagata attttaaacc taaagaaata aaagaatcaa tttctatacc taaagaacaa
     9061 ttaaatatat tgccaaataa atttttaatt gcatctattt ttgtaaccac attcattact
     9121 caactagctt tatactctat agaaccaatt gtaaccatat atatatcaca acttacaaat
     9181 tttgcatcaa atgtagcttt aatagctgga ttgacatttt ctgcatcagg attagcaaat
     9241 ttattagcag ctcaaaaact tggaaaaatg tcagataaaa taggtcctca aaaagtattg
     9301 ttaatttcac ttttatgggc tgggattata tttataccac aagcttttgt aaaaactgca
     9361 tggcaactga tgttgttgag atttttatta gggttgtcag ttgcagggct taatccttct
     9421 gttaacagct tgcttaaaaa aattgcacca gaagaatatg tgggaaaaat atttggatat
     9481 aatgcatctg cccagtatat aggttgttca tcaggagcat ttttaggggg acaaatatca
     9541 gcacatttag gtattagaac agtatttttt tcaacaagtt tattgttgtt tatgaatgca
     9601 ctttggatgt ataaatttac tggattgttt attaaagata agactaaata atcatactat
     9661 ttacatttct aattttatga tgtaaaataa atatttttta tgtaatttat catagaatta
     9721 tataaaaaat aactctatgg taaaaattgg aaaatattat tatcgacaaa atctttgact
     9781 taaaaactat attgcgatag tattaattta ataaaagttt gagaggggcg taaggttatg
     9841 acaatttcaa aaataccaca agcagaatta aaagttatga agtttatttg ggaaaaaaat
     9901 gatacagtaa catcaaaaga aactattgaa gctatggaac gaaaatatgg ttggaaacaa
     9961 acaactacat tgacactttt atcaagactt gttaaaaaac attttttaag tgcagaaaaa
    10021 atagatagat acactcatta tacaatagtt gttggacata aggagtattt aagtgttgag
    10081 acaaaagatt ttttaagcaa tatacacgac aattctttac agagtttaat ttcagcacta
    10141 catgatgatg aagttgttga taaagatatg ttggatttat ttgaaagtca ttttaaaaat
    10201 ttagaagaag agtaattata tcaggataaa actctaagaa tttaattaat ctatggctga
    10261 aaaccataaa tggtattatt taatcagcta ctcactattc ataaagtaat attaaatagg
    10321 ttctctaatt agtaaaatac ctaaaataat tggaatagg
//
LOCUS       DAEPOG010000136        10341 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEPOG010000136 DAEPOG010000000
VERSION     DAEPOG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.136"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1906)
                     /locus_tag="KR432_002793"
     CDS             complement(<1..1906)
                     /locus_tag="KR432_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0641312.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFL"
     gene            2140..2592
                     /locus_tag="KR432_002794"
     CDS             2140..2592
                     /locus_tag="KR432_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0641313.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            2595..2903
                     /locus_tag="KR432_002795"
     CDS             2595..2903
                     /locus_tag="KR432_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0641314.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            3017..4123
                     /locus_tag="KR432_002796"
     CDS             3017..4123
                     /locus_tag="KR432_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0641315.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            4198..6876
                     /gene="mngB"
                     /locus_tag="KR432_002797"
     CDS             4198..6876
                     /gene="mngB"
                     /locus_tag="KR432_002797"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0641316.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7193..9526)
                     /locus_tag="KR432_002798"
     CDS             complement(7193..9526)
                     /locus_tag="KR432_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641317.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9501..10217)
                     /locus_tag="KR432_002799"
     CDS             complement(9501..10217)
                     /locus_tag="KR432_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641318.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3857 a         1450 c         1343 g         3691 t
ORIGIN      
        1 aaagaaaatt atcaaaatca ttacaattag atatactatc tctaatatca ttgtcatgaa
       61 ttatctttaa aaggatatca taaatggagt ttatttttat ataatcatct ttatttatag
      121 ctaacatcat gattaattta acagtagaat cttcactcca atatattggt tcattcaata
      181 tggaaataca aatcttagtt cttatagagc ttaattccat aggatgaggt atagctaaaa
      241 aatcatctat attggtagaa gataaacttt ctctgtaaaa tacagattca gcaaatgatg
      301 gaaatacaat ttcactttga gttaatttgt tgcacatcaa ctttataagc tcatctttat
      361 ctttgatttt aggattaaca ataaataaat ttttatcaaa gaaattgtcc aaaagattac
      421 tataaatatg ggaattatta gtgattgact tagatatatt ctctatatct ttttttaata
      481 gttttaaatc cactaataca ataggaatac tatcatgatt taaaggaatt gtcgatatta
      541 ttatatctac atctgacaat tcactagcta aaaattgatt aaatgataga cattgaagta
      601 tatttatttt atcatgaaaa actttgttta attgaacctc taataaccta cttgaaccat
      661 accctgaacc acatactaat actacgtttt tacacttaat attattttga aaaaatctct
      721 ctattccagc acctatatgc agggatacat atccaatttc atcttctgtt aaagagtaaa
      781 tactattttt aaaaactttt tctatagcat ttaaagttat ttcaaatgct aaaggataat
      841 tactctttat tgtatttata aggggattca ccttatttaa attataagat ttagaattga
      901 gaattgattt aaagtgcagt actaaatcat caaataatac actatcattt ctaaggtcaa
      961 aagtatattg attgttgatt acttctaaaa tttcttcaac atactctata attttagtat
     1021 ccaccctatt ggaaacgtta tccaaaagag aacttttagt tataaaatga ttatatagat
     1081 aaactctgtc tgcctttgaa ataattatag tatatttact ctcaatataa ttaaatatat
     1141 tttcaacagt tgtattcgat tgaaaatctg ttaaaacaac atctaaaaca ttatctaagc
     1201 agtaaccatc aagtattcta cttatagtaa tagctaaatg gataataaag ttttttagat
     1261 taaaatctag aaaatttata ttaaatttat taatttcact taacactacc ctttgaagct
     1321 ctattaaatc tacattatta aataattcta tttctctatc tgtaattcca attatatagt
     1381 tttcaaaatt cctatttatc atattattag aaatacatct tcgaatatct atttccttcc
     1441 cttcaacttt aactccataa tatggcttag aaactaatat taaattatac tttgacaaaa
     1501 taggctttat tctttttatg tcatttagta ttgtaaattt actcacatac aagctattag
     1561 ctaaatcttc tagttttata tatgtattgt tatagagaat atattttaaa atatattgat
     1621 ttctttctat aggagaatct ggtatttcac ttgaatccat aatatcatca gatattaatg
     1681 ctagatactg ttggtataat gctaaattaa gaatatctat ataatatcct tctccttttt
     1741 ttattttaat aatagcacca tttttctcta aagttccatt tatagaagta atatcatttc
     1801 ttatagttct ctcactcact tttaaatgat tacttaaagt tttacaagta attttatcat
     1861 cactttcatt taataattta aacaacgtga gcaatctctt gtgcataaat atcccccctt
     1921 atagaatttc ttctaaatta tactatttac actcatataa tacaattact ctaataaaat
     1981 tgtattataa taatttcttt ccacttctat atggaaatta gttattatca taatataaat
     2041 ttccatgtcg aagtggaaat tagttatatt taatttaatt aggataatga ttacaatata
     2101 aatataagtt aagacattac gaatggagtg gaaaaatata tggtactaca agaaaaatat
     2161 atatttatag ataaagactt aaaaagcaaa gaagaagtat taaattttat tgcagatagt
     2221 gcttatatac taggtattgc aactaataaa gaggctcttt taaaagacct tctaaaaaga
     2281 gaaaatgaat ttccaacagg aataaaagat ggatttgcga tacctcatac aaaaagtgta
     2341 aatgtaaagg aagcttccat actctatatt aaaacaacta acgagttaga gtggaaaact
     2401 ttagatgata gtaaggttag atatgtattt agtttgttag ccccacataa aaatgaagga
     2461 aatatacatt taaaaactct atctaaatta gccacatgtt taatggagga tgattttaca
     2521 agtgaagtta aaaatgctag tgatgcaagt catttagtaa aatatataac taataaaatg
     2581 aaggaggaat aattatgaaa atagtagcaa taacatcatg tatagcagga ttagcacaca
     2641 cacctatggc agcaaaagct ttagaacaag ctgcatcaaa aatgggtcat gaaatcaaag
     2701 tagaacaaca aggtgctatg ggacgagtaa atactataac aaaagaagaa gcacaagatg
     2761 cggactttat acttatagca tctgaccaaa ctatatctgg aatggaaaga tttgatggta
     2821 aaaaagtgat aaaagtcaaa ataggtatag cgttaaaaaa accagaagct gtcttaacaa
     2881 agtgtataga agctatttct taataaaaaa atctcactct aatatagaat gagaaaaata
     2941 aaaaattaaa aaattgtcta atgtaattat aacatattat caaaaaatat aacaaactta
     3001 tataggagga ttaaaaatga gtaaaaaaaa taaatctctt ttaagtgaac ttaagggaca
     3061 cttaatgaca ggtatctcat atatgttgcc acttgtaata ggctcatctt tagttgtagc
     3121 tatcccaaag cttacagctt tagcaatggg tataacaagt ttagatgctt ataaaaatgt
     3181 atctggtttt tatcatattc tttacttaat ggaacaagtt ggttggactg gcataggtct
     3241 attaaatacg gtattagcag gatttatagc attttcaata ggtgagaaac cagctatggg
     3301 tgcaggtttt attggtgggc tcatagcaag taatactaat gcaggattct taggagcagt
     3361 aatagcaggt ttctttgctg ggtatgtttg taagttttta aagaaaaaaa ttaaaatatc
     3421 tgggtcagca tcaggaatga tgccacttat aataatgcca cttataacag ttggattaac
     3481 tggtttttta atgagtgttg tattagcagg acctttagga aacataaata cttcattaaa
     3541 tgcttgggta gctgaaatgt gtcaaaatgg aacaaattcg gtggtattgg ctttaatttt
     3601 aggcgcaatg ataggattcg atttaggtgg accagtaaat aaagcagctt ggatggcagg
     3661 aaatgcatta ttgttagagg gaatctatct accagcgata ttggtaaact gcgcaatagt
     3721 tatacctcca ctaggatatg gtttagctac attcataaga aaatcaaaat tttccaatgc
     3781 attagctgaa acaggaaaag gcaatatagt tatgggtata attggaataa cagaaggtgc
     3841 aattcctttc actttaacaa atccattaaa attagttcct gttaatatga taggatgcgc
     3901 tttgggtgta ggattatcag ctttgttagg tgtacatgct ataatgcctc cagttggtgg
     3961 attatatgga tttatatcaa taggttctgg atgggcttac ttagtaggtg caatatttgg
     4021 agctttagta atagcaatat tatcaacaac tttagtagac ttcaatgaag atgaaactac
     4081 ggaaaataca gaatttgatg aaatagagtt agatataatg taaattaaaa acttaatttt
     4141 agataattga gaattgagtg tactctcaat tctcaattaa attttgggag gatttttatg
     4201 aaaaagacaa aagtacatgt agttccacac agtcattggg atagagaatg gtattttaca
     4261 acatcaaggt caaaaatata tttaatgaaa gattttcaag atatattaga catattggaa
     4321 gaaaaagagg actttaaata ctttactatg gatgcacaag cttctctctt agatgattat
     4381 ttaaaatgga gacctcagga tgaatataga ataagagatt tagttcaaag aagaagatta
     4441 gttattggac cttggtatac tcaaacagac caattggtta tatcaggaga atctataatt
     4501 agaaaccttt actatggaat agatagatgc aatgaatttg gtgaacctat gagggttgga
     4561 tatgtaccag attcttttgg gcaatcagct caaatgccac aaatatacag aggttttgga
     4621 atagatagtt cattattttg gcgtggagtt tctgatgata tggtagatac aacagagttt
     4681 atatggaaag gttctgatgg ttcaaaagta ttagcagttc aaataccatt tggttattac
     4741 tatggaggta atataccaga agatgattca gatttaaaag aatatttaaa agatataatt
     4801 ggaaaactta aggttaaagc ttctactaat aacgtatatt ttccaaatgg atttgaccaa
     4861 gcaccaataa gaaaaaacct tcctgatata ttaaaaaaag ctaatgaaat agataatgaa
     4921 aatgaatacg aaataagtaa tatagaaaac tatatagatt cagtaaaaaa tgaaagaaat
     4981 gaatttacag agcttaaagg tgaattttta aatgctaaac atatgagaat acacaaatcc
     5041 atattctcct caaggtcaga ccttaaaata atgaataaca aattagaaaa ttatatcaca
     5101 aatgtacttg aaccattatt aacattaagt tattcacttg gaaatgaata ccctcatcta
     5161 gttataaaag atatatggaa acttatgttt gaaaatgcag ctcatgacag cataggttct
     5221 tgtaatagtg acacagcaaa tgaggatgta tacacaagat acaaacaagc cagagatttg
     5281 agtataaatt tattagattt gcatatgaga cttataagta ctcaaatcaa aaataataat
     5341 gaagaaataa cattaacagt attcaatgga ttcccagaag tcagaaatga agttgtagaa
     5401 tttgacactt atattccagg tcaagatttt gtgataaaga ataaaaatgg agaaattctt
     5461 aattatataa taaaagaaaa agaagatatt acaaattata tattaactca aacaataaga
     5521 ttaaacccaa gtaaaaaaat atatataccc aaaactgtat atagagcaaa aattgcactt
     5581 tcagtaaaag atataccacc aatgggatat actcaactag tttttgaatt ggatactaaa
     5641 aataaatgtg aaatagaaac aagtaattgc aaagaaatag aaaacaaata ttacaaaata
     5701 acagccaata aaaatggttc attagaaata ttagataaag aatctagtaa gacttataaa
     5761 aaccaagctg taattgaaga caatggagat gatggagatt catacaacta ttctcctcca
     5821 agaaaagata tttatgtttc atctttagat tctgtatctt cagtagaagt gctaaaatca
     5881 aatattcaag aagaaatgat acttaaatat tcattgacca taccagcttc actggaagaa
     5941 aggtctatag gtaaagtttc tgtgaaaatg ccagtaacaa tgaaaattac cttagaacct
     6001 aaagaacaaa taattaagtt taatgttaat ataaaaaata accaagcact atctcataga
     6061 gtaagagttt tatttaatac agaaatagca tctaagtttt ctatagcaga tcaacaattt
     6121 ggaataatac aaagaccaac agtattagaa aaagaccttg cattatggaa aagagacaat
     6181 tactcatggc aagaaaaacc tataactatc gaaccaatgc aaagttttgt tactttagag
     6241 gatggacaaa gaggaatagc attaataact ggttgtgtaa gagaatatca aatagttggt
     6301 gataatttag atacaatagc attaacacta ttcaggtcat ttggatatat gggaagagaa
     6361 aatttacttt atagaccagg aagagcttct ggagaaaaaa taatagcaac accagatgca
     6421 caattactta aagagttaaa ctttgacttt ggtctttaca tatataacaa taaatttgat
     6481 gaagcaaatg tagctaatac agctaagaga ttcttaacac ctatacaaat atatgaatat
     6541 gctgattttt taaatggtag acttctattt gcattcaatg acgaaaaaca aatatatgaa
     6601 aatgaattta gtttgatgaa tgtaaataat tcaaatttca cagtaagtgc aataaagaaa
     6661 gaagaaaaag gagatggaat tgtagttaga atcttcaatg gtatgaaaaa tgaggatgct
     6721 aagggaagtc ttaacatcaa taaaaatgtc aatgatgctt atagtgcaat gctcgacgaa
     6781 atctatgata ataatagcaa gcttaaagta aatactaaaa ctgtagaggt aaattcttta
     6841 tcacattgta aagtacaaac aatagtaatt aaataatata aaatagctta tacaagattt
     6901 caaaataaat tgctagtact atgttaatag taaacccaga tattatgcta tttataagct
     6961 ctgtaacact aaataacatt ttgtaaaata aaaaggtgaa ccttgtatat taggattcac
     7021 cttttttaaa taaatcaaca ttctgtttta aaataacatt tataagctgt tttgaaataa
     7081 tacttacaaa ttattttaaa acaataccta taaattttta tttaaagaat acacataaat
     7141 atttccagac tcttttattc tatagacttt ataattatta aagaagtcta atctaatttt
     7201 tgttaaaagt aattgtaact ttaaataaat caccatccac acttatattc atcttaccac
     7261 cctgtaattc tataaaactt tttgctattg ctaaaccaag accagaacct tctgtgttac
     7321 gtgatttatc tcctcttaca aatctctcca ttatatcatc tacactaaaa tcaatctcag
     7381 atgcagacat atttttaagc ataatattta ctttattatc tgtttctaaa atatcaatat
     7441 acactcttga atttggcatt gcatatttgc ttatatttat tataagattt tcaaatactc
     7501 taaatgtacg ctgactatct aatggtaata ttattttttc attagaaaag ttattcttta
     7561 ctattagaga tgaatctgca agtttgtcat ctaattctaa aagtgtctgc ttcattagag
     7621 atacaatatc cacatttact atattcaaat aaacatctcc actatttgcc ttgcttactt
     7681 caaacaaatc ttctattaga tgttgcaatc tttgtgactt tctgtctaat gtatcaatat
     7741 acaacttacg attttcatct gttatacttt catcttttaa taaatctata taagtgatta
     7801 tagatgttag aggtgttttt aagtcatgtg atacatttga tataagctct gttttcatct
     7861 tttgactctt aacctcttcg tcaacagctt ttttaaatcc agtttgaata tttcctaatt
     7921 cgtcttttat aggattaaaa aatcctaaat ctttttccat atttacatct aagtttccat
     7981 ctgaaatttt ctttgtaaca tctaaaagtt gattatattc atatattgtc tttttagagt
     8041 aatctgacag tatcttaaat aatactactg aatatattaa tgcgagtatt attccaaaga
     8101 accacatact acacattata actataatca ataaatttat tcccaaaatt gtaactatag
     8161 ttttatttgg cttttctttt agatttattg ttctaatttg attaataact gtttttcctg
     8221 ctttttttaa ccatacaaaa aacattcctg tcacagtatg ttttgtaaaa tactctctta
     8281 ttccaacatt taaaatatgt tttaaaagta ttataaatac aaatattatt gagaatatta
     8341 caaaccaata agttaaattc attaaactta ccaatatgct agccatttta gcacttatgt
     8401 cattgtttat aaggtactct acataattcc cactcaaggt attacttatt ataacagtag
     8461 gatataaatc tacaagcccc tgtataatag caagagcaat aaagagtatc tctaacggta
     8521 ttcttaataa tgtatcaaat cctagcaact ctttagattt tctatatggg aatgctaaag
     8581 ctacaataac aactatagct aatataattt ttaaaatcat tccagaaatc ttataataag
     8641 aattattttc tacattgctt atatatgaaa ttataccatc atcaaaataa gtatttccat
     8701 aaccatactc tacactatca cctttactta ttactttagg tacagcatat actaatgtta
     8761 tattttttat tggagataac ttatatgaaa ttgaatttcc tcctaaactt gtatccatta
     8821 aatcttctct tgctttagat aacaacattt gattaataac tttttgattt actccataag
     8881 aatccacaat atttactttt ccatctttat caaaattcat tacaacgtag aatctatagt
     8941 tttcctttat atctgtaatc tgcttttgtc tttcctcatc attcatttct gtattttttg
     9001 actgattttc atcagaattt tcttttgaag catcattctt tgttgtatca agtactccta
     9061 attctttatt atcattaaaa aattcaaaat tgctttcttt atcaactaaa tagtacttta
     9121 aattctttcc tgacgcttta atgaaatgct cccaattttc aaagttttca ttaaaatctg
     9181 cattaatact atcatctgta taataagttt ctgaaccatc ataagtatct ttttctttac
     9241 ttacattttt tatatagttc atagtttctt cattgataaa tagctttgat ggttgtatca
     9301 tcttaccttc ttgttcttcc ttcatattat gatataaaac ataacttcct ttatatatac
     9361 tatctacaaa gctttcctgt tcaaatggat tctttgctcc acctttagca cctgcttgta
     9421 tagtagaata ttgactaatc atacctaatg atgctgataa cataagtata aaaactataa
     9481 tagtaactat atatttatct ttaaattttt tcaattttgt atccaacacc ccacaccacc
     9541 tttaaatatt taggattttt tgggtcaatt tctatctttt cccttatatt cctcacatgt
     9601 accataactg tatctgtatt tatagcattt tcattccaaa ctctttcata tatttcatca
     9661 gcagaaaaaa ctcttcctgc atttctcata agtaaactta gtattttaaa ttctatagga
     9721 gttgttttta tatattttcc atcaatatta acttcttttg tattttcatt taattctaat
     9781 ccgcctattg caaacacact atcatcactt tctacctttt gctctttatt tccaaccatg
     9841 tttagatact tagtgtacct tctaagttgt gaattaactc tagccaatag ttctaatggc
     9901 ttaaaaggtt ttgttacata atcgtctgct cctatattaa gccccattat tttgtcaact
     9961 tcttctgatt ttgcagatag cattattact ggaaaattat agttttctct aagcctcatt
    10021 gtaagtgtta taccatccat tttaggcatc attatatcaa ctatagctaa atgtatttct
    10081 tggttttcta taaccttaag accttctaat ccattgtatc ccttaaaaac attatatcct
    10141 tgatttaata gatatatctc tatagcatct gcgatctcct tttcatcttc taccaccaaa
    10201 atattatatg aattcatttt aatctccttt atattaattt ataaattttc tttacatata
    10261 tgattataca atatgataga agtttttcca tttatatata tatttatatc ttttgttctg
    10321 gttatttgta tttaatgtcc a
//
LOCUS       DAEPOG010000137        10305 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEPOG010000137 DAEPOG010000000
VERSION     DAEPOG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.137"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..113
                     /locus_tag="KR432_002800"
     CDS             <1..113
                     /locus_tag="KR432_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH0641319.1"
                     /translation="FDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            185..925
                     /locus_tag="KR432_002801"
     CDS             185..925
                     /locus_tag="KR432_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBH0641320.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            1125..2276
                     /locus_tag="KR432_002802"
     CDS             1125..2276
                     /locus_tag="KR432_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0641321.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            2301..3056
                     /locus_tag="KR432_002803"
     CDS             2301..3056
                     /locus_tag="KR432_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBH0641322.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            3248..5635
                     /locus_tag="KR432_002804"
     CDS             3248..5635
                     /locus_tag="KR432_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBH0641323.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(5866..6099)
                     /locus_tag="KR432_002805"
     CDS             complement(5866..6099)
                     /locus_tag="KR432_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0641324.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            6388..6978
                     /locus_tag="KR432_002806"
     CDS             6388..6978
                     /locus_tag="KR432_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641325.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            7092..7937
                     /locus_tag="KR432_002807"
     CDS             7092..7937
                     /locus_tag="KR432_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641326.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            7934..8611
                     /locus_tag="KR432_002808"
     CDS             7934..8611
                     /locus_tag="KR432_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641327.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            8608..9324
                     /locus_tag="KR432_002809"
     CDS             8608..9324
                     /locus_tag="KR432_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641328.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            9421..9684
                     /locus_tag="KR432_002810"
     CDS             9421..9684
                     /locus_tag="KR432_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641329.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            9656..10111
                     /gene="def"
                     /locus_tag="KR432_002811"
     CDS             9656..10111
                     /gene="def"
                     /locus_tag="KR432_002811"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0641330.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            10163..>10305
                     /locus_tag="KR432_002812"
     CDS             10163..>10305
                     /locus_tag="KR432_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641331.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLN"
BASE COUNT         4067 a         1055 c         1795 g         3388 t
ORIGIN      
        1 tatttgatac ttttaaacta ggattagaag actataaaat tatttataga aatgcagtag
       61 aagctagttt tgcagataaa gaaactaaag agtatttgaa tagtttaatt taatagataa
      121 aaatatttaa actttaaatt aagctattaa attgacaaaa atacattgta ttaggagaaa
      181 aaatgtgaaa gattatgaag atgttgttta tattaaacta gtcaaaaaag aacttgaaga
      241 atggaagaaa aatatattaa agaaaccttc tatatttgac aaagcatcaa agggtataca
      301 gaataaattt aatggagtat tgccagataa atatcatgaa ataattactt cttctataaa
      361 agtaatgaca aaaaccgtac tgtttggata taaatatgct acaaagccac cacttagaaa
      421 tatgtcatta gaagaaagag aaaaattagt tttagaaaaa gctaaaactt acaagaccac
      481 agcaatgatt gaaggggcag gaacaggggc aggaggaatc ctcttaggtt tatctgattt
      541 tcctttgctt ttaggtatta aagtgaaatt tttatatgat gtagcttcaa tatatggata
      601 tgatgttaaa gattatagag aaaggattta tatattaaac atattacagt tggctttttc
      661 tagtcaacag catataaaca aaacttttaa attgatgaat aactgggatg aatatgtaaa
      721 tagtctacca gaagacatca atgattttga ttggagaaca tttcaacaag agtatagaga
      781 ttatttagat ttagctaagt ttttacaact aatgcctgga ataggagcat ttgtaggatt
      841 ttatgtgaat ggaaaattaa ttgataaatt atgtgaaact gctgtgtctg cttatagatt
      901 gaggattcga gagcttaagt tataagagaa attataacat aaaaaattag ttatacaata
      961 tatctatatg tatctaattt tgcattgata aaaacaatta tggataaaag gtaaagcatg
     1021 atagaatcta atgtagaggt ttgtatttta atggaaaaat ttagttttta ttgcttaaga
     1081 tacaaattga attacgaaga gaagtaattg ggaggaatat tttcatgaca aatttgccaa
     1141 aacaatttga tagcttcaat gaagctagac aaaagggatt tataaatgca aaggaactta
     1201 aggaaagtgg gaaaaagatg gttggagttt tctgtacatt tactccagtt gaaattccaa
     1261 tggcagctgg agcaactgta gtaggtgtat gcggtgttag tgaagagcca atacctgatg
     1321 cagaaagggt gcttccaaga aatctttgtc ctttaattaa atcaagctat ggacatgcaa
     1381 taacagacac ctgtccttat ttctattttt ctgatttgtt gataggagaa accacttgtg
     1441 atggtaagaa aaaaatgtat gaagaacttg caaaagttaa gccaacatat gtaatgcatt
     1501 tgccaaatac ttcaaaagga gaatttgcct ataaactttg gaaagatgaa atgataagat
     1561 taaaagaaga ggttgagaaa tctttaggtg taactataac tgaagaagat ataagaactg
     1621 ctataaaaga taaaaatgaa gaaagagaac ttttaaaaga gttttatgca cttggtaaat
     1681 tagaaccatc tgcacttaca ggccttgagt tacataatgt tttatatcaa gcaggtttta
     1741 aatttgaccg tgcagaactt aaacattctt taagaaaagt tatagatgat atgaaagaaa
     1801 gatatgaaaa aggtgaatgt cctgttcaaa aagataagcc aagaatattg ataacaggtt
     1861 ctcctattgg tggaataagc gaaaaaatag ttaaaacttt agaagatgca ggggcatcag
     1921 tcgttgcata tgaactttgt ggagctatta gatcaaacga tttattggtt gatgaagaaa
     1981 tagaagatgt atatgatgct ttaacacaaa aatatataaa tataggttgt tcttgtatga
     2041 tgaataatga caatcgtatt gaattattag atagaataat agatgaatat aatgtagatg
     2101 ctgtaataga tgtagtttta caagcatgtc atacatttaa tatagaaagt tatagaataa
     2161 gagaatttgt tacaaaagaa aaaaataagc catttatgtc tcttgaaact gattattcaa
     2221 aatcagatac tgaacaatta agaactagat ttgaagcgtt tgtagagatg ttgtaggaat
     2281 aaataaaagt aggaggaact atgtttagta taggagttga ctcagggtca gtggcaacaa
     2341 agggtgtgct atttgatggt gaaaaaatta ttaagaaaat aattatacca actggatgga
     2401 gccctaaaaa tacatctaaa caggtctatg agttgctaag tagtgaaatt gacaaaaaag
     2461 atataaaaaa ggtggtaggt acaggttatg gaaggggagt aatggatttt gctgataaga
     2521 aagtaacaga aataacttgt catactagag gtatttactt tttaaacaag aacataagga
     2581 ctattttaga tgtaggtggt caagatagta aagttataaa tttagataga gatggaaatg
     2641 tatttaactt tataatgaat gataagtgtg ctgcaggaac tggtcgattt ttagagatta
     2701 cttctaacct tttaggaagc gatattgaaa gtatagatac attagctaaa ggatatgaac
     2761 cagttaatat atctagtatg tgtacagttt ttgctgagtc tgaaattgta agtcttctag
     2821 ctcaaaacat ttctactgga gaggtagcag cagggatact taaatctatt gcaaataaat
     2881 ccacctcaat gttagcaaga ggtgaggtaa tagatgaagt tgcatttact ggagggcttg
     2941 caaaaagtaa agaactagta aaaatgatag aagaaatact gggtaaaaaa atttttattg
     3001 cagaagatac tcaaataata ggagctttag gagctgctgt aataggattt agataaaaac
     3061 atataaaaag aaaatgaaaa gctgaattta tcataaataa gttcagtttt ttattttatt
     3121 ttgtttattt ataaaggaaa actttggtat aattgattta aatgattgta tatttgtaaa
     3181 tacttgatat ttagtcatat ataacattag aattttaaaa attagctcaa aataaggggg
     3241 agaaagcgtg aaaaaattta cttcaaaaaa agtagcaagg tttgttgcac tgtttttaat
     3301 atctatttta gtaatgtcaa caattcctgt gtcagctgac aatagcaata cattgaatca
     3361 gataaaaaaa gcagaataca gtgatactta taaacaatat ctaaatgatg ctaaaaatgg
     3421 acgtacagaa aaatataatg gaattatacc aaatccatat cagttagaag gtactcaaat
     3481 tcaaagtaaa ggaagaacag ctccaacatc ttatgaccct agaaaattag gtcttatgac
     3541 atcagtaaaa aatcaagagg atttaggaat atgctgggat tttgctgcaa tggcaacctt
     3601 ggaaagtttc ttgaagttaa ataattatgg agactacgat ttatcagaag aacacttaag
     3661 atggtgggct tcagatggtg aatatggatg gtctgtaaat gatatggatg gtgctttaaa
     3721 ttatgaagct atgggatatt taacatcttg gtcaggtcct aaattagaaa aagatatccc
     3781 atacaatggt agagtatcta aagcacaagg agcaaaaaaa ccaactaata tgaatacagc
     3841 acctactgtt tttaatgtga cagatgctgt gtgtgtaagt aatgacataa attctacaaa
     3901 aaatgctata ttacagtatg gagcagtaac atctggttat tatgaagata ttaaatatca
     3961 aagtgatgac caaaattcat attattgtcc aacaaaaagt tttaatacaa atcatgctat
     4021 ttcaattgtg ggttgggatg ataactattc taaagataat tttaatgcaa atattagacc
     4081 atcaaaaaat ggtgcttggt taataaaaaa tagctgggga gattataatt ctgagggtgg
     4141 atatttctgg atatcttatg aagacaagac acttatgtct gatatagata atttttcaat
     4201 caaaggtggt aaaaaaccaa atgatgatga gaagatgtat caacatgact atgcaagtat
     4261 tgtaccttta atttcaaaaa agataacagc tgcaaatgta tttgatttca atagaggcga
     4321 tgaaactcta aaatctgtaa tgttcttaac tgaaagtata ggagctaagt atgaagtatc
     4381 ttatgctcca gtagtaaatg gtatacctca agaaaacaat atgaaaaaat taaaagaagg
     4441 tactgcccaa tattcaggat acataacagt tccaattgat tcttttaata taccagaagg
     4501 aaagggtgca attgtagtca gtatagaggg gaaaaatgga gagtcaacta taggttctga
     4561 gtcaaatgta ccaggatatg atacatttaa agcaaaagct aatttaggtg aaagttatat
     4621 catagataat actggaaaat tctttgatat aaatagagat agtaacttct atccgtgtaa
     4681 ttttactata aaagctgtta cagaaaagtc ttctggtgag agtatcccga atgagtcttt
     4741 aattggtagt gatagatatg aaactgctat caaagttagt caaaatggtt tcaattcttc
     4801 agaaaatgta gttttagtaa atggcagttc aatagtagat gcattggcag caactccttt
     4861 tacatctgct ataaattcac caatattatt gacacaaaaa gaagctttaa attcaaaaac
     4921 taaagctgag atacagagat taggtgctaa gaaagtatac ctaataggcg gagaaaattc
     4981 tataagtaaa gaaattgaac aacaattaaa aagcttaaat atatcgatag agagaatatc
     5041 aggaagtgat agatacaaga caagtttatt attagctcaa aagttaaatg gtataaaaaa
     5101 tgtatctcaa atagcagttg taaatggagt gaaaggatta gctgatgcaa ttagtgtagg
     5161 tgctgctgca gcagaaaata acataccaat catacttgca aatgaaaaga gcgaattgca
     5221 aggagctgat gaatttctaa attcattaaa tatagagaaa tcttatatta ttggaggaac
     5281 agcatcccta tcaaacaatc tagaaagtaa gttaaaaaat ccaactagat tatctggtag
     5341 tagtagagac gaaactaatt caaagataat agataatttt tataaaaaag atactttaaa
     5401 aaatgcattt gtagttaaaa atggaatcaa gaatcaaaat gatttaatag atggattggc
     5461 agttggtcct ttaggagcaa aaactgggtc accagtaatt ttagttggtg ataaattagc
     5521 tgatagccaa aaagaagttc ttaaaaataa gactttagaa aaagtaactc aagttggtgg
     5581 aggagcaaat aaaaatgcat ttaaagagtt agtaaaatta aaaagtgcta aatagagtac
     5641 ttaagtatta acatatatta gtgtgtatta gtaacttatc ttaataaaag ctgaaaataa
     5701 ttactttaaa ttattgataa ataagataat caatgattgt aaagttttgt tttcagcttt
     5761 aaatttatgt agtataagtt taaaaacata aaaaagggtt actatatata gaaatataga
     5821 aaatcttata ttttatatat aaataaccca tatttaatta actttttata gtgttctaat
     5881 agatattatt gaacttaatg gtaaatataa tgtgtaagtt tcatttaaag ttggattaga
     5941 ttcatctata tatcttgagc aaagtgtaat tgataagcta tctgcttcta taatttcacc
     6001 atatagagga caactgctaa agtaagaatg tacttcaaca tatttattta agttttcttg
     6061 aacataatcg taaaaacggt tttttgtagt agtagccata aaatcaactc ctttattttt
     6121 tattaaagtt gaaactttta aaaagataaa tatgagtact tctattagaa actaagttaa
     6181 aaaataagca aatagatatg aaaattagaa taatatatta tttatatctt aattataaca
     6241 ctagataata aaaaatgcta tataaaattc agaattttcg aaagttttta agcttatttt
     6301 taaatatact aataatacta ttgacattag atagaaaaag tattatattg actatataaa
     6361 ttattttagt caaactggag gaaaaagatg cctaaatcgt attctgataa agaaagagag
     6421 tatataataa aaagacttaa agaagaagca agattgtgta tggagcaata tggtattaga
     6481 aaaactactg ttgatgagct tgtaaaacgg gtaaaaatac caaaaggaac tttttatcta
     6541 ttttttcaat caaaagaatt gctctttttt gaggtattga gagatataca tgactctata
     6601 caaaaagaaa tcttatatga gataaataga gtagatgaaa gtattacatg tgaacaattg
     6661 acagatatat ttatgaaatt ctataggatg gttgatagta cttcaatttt aaatctcatg
     6721 ataaatggag aatttgagat attggtgaga aagttaccag attctataat tgaagaacac
     6781 tttagacatg atgactttga aattggagag attatatctt gcataccaaa tgcaaaaaat
     6841 aaagacatag aaagctttag tggagcattt agagtagttt ttcttaccat gctatataag
     6901 cgcgaggttg gaagtaattg ttttgaagat gctttaagat taatgataaa cggattagtt
     6961 atacaactta tggaatagaa tttaaatttt gtttgctaat aacttttatt aaaaattatt
     7021 aacaaacaaa aaaattatat tttattgacc atatagtcaa aatagtcaaa aatttgaaag
     7081 ggtgatattg aatgattaaa gttgatgatt tatcatttag ttatacagat agaaattttt
     7141 tacaaaatat taactttgaa gtagaaaaag gggaaatact aggattttta ggaccatcag
     7201 gtgcaggaaa atctacatta caaaagattc taattggaat gataactaat tatggaggaa
     7261 gtgtcattgt aaatggagta gagagtaaaa gacactcaaa taaattttat gaaaatatag
     7321 gagttgattt tgaatttcct agtttatatg aaaagttgac tgcaatagaa aatttaaaat
     7381 attttgggtc tctatattct aaaaaactac tatctattga tgagttatta aaatcagtag
     7441 gactagaaaa tgaagcaaat aaaagggtat ctgagtattc aaaaggtatg aaatctcgat
     7501 taaattttat aaaagcttta ttacataatc cagatatttt atttttagat gaaccaacaa
     7561 gtggtcttga cccgtcaaat agcaaggtta tgaaagatat aattttatca gaaaaatcta
     7621 aaggtaaaac tattatttta acaactcata acatgttaga tgcaacggag ctttgtgaca
     7681 gagtagcgtt cattgtaaat ggtaaaatat ctgctttaga tacaccacat aatcttatta
     7741 tgtcaaaagg agctattaaa gttagatata cttactttga taatggagaa aaaacttctg
     7801 aatgttttct gaacaatact gctaatgata aaaatttgaa tatgcttatt aagaaaaata
     7861 aacttttatc aatacatagt agtgaaccaa cattaaatga tatttttatt gagataacag
     7921 ggaggaattt acaatgaggc taaaaaatct tattttagga gatataaagt ttcaatttaa
     7981 gtatggtttt tattttttat atcttttttt aagtattata tatatttgta ttatcaatgt
     8041 atttccaact tttatgagag aaaaaatagc tataattatg atttattctg acccagcagc
     8101 aatgggattg ttttttatgg gagctattgt acttcttgag aaaagtcaaa gggttttaaa
     8161 ttccttagct gtatctccag taaaagtatc tgagtatatt ttatcaaagg taatttcatt
     8221 aggagtaata tctagtattg tagctatgtt tattgctatt acattaaatt tagataatat
     8281 tataatatca actataggta cctttttttc ttctattata ttttctttgc ttggattgat
     8341 acttgcaagt aaagcatcta gtttgaatca gtttattgtt ttaagtattc ctattgagat
     8401 tatatgcttt attccaccaa tactcaatgt acttcttgac acaaaatcat atgcaaattt
     8461 gtaccctttt aatatatgta tttctcttat tagtggagat aaaaatttta taatgattaa
     8521 tattttaata tcaattataa taataattta ttttataact tattatttta tttgtagcag
     8581 ttggaaaaag gtaggtggag taaaactatg ataaagatta taaatgcgtt taaacaagag
     8641 tttgaacaaa ttaagagaga tgctatgctt tttatagtat gtgtttctcc aatattatgt
     8701 ggagtattta taaaatttgg aataccatta atacagaata tatcacttaa caagttttat
     8761 tatcaattaa atttagagcc gtatttcttg atgtttgatt tacttttagc ttttattaca
     8821 ccttttatgt tcttttttgc ttctacaatg gtcatattgg gagaaattga tgacagcatt
     8881 tcaagatatt taatagtaac tccgcttggt aaaactggtt atcttatttc acgatttgga
     8941 ataccaggta ccttagcatt tattattact atgattctac ttatcttttt ttctctaact
     9001 aaaatttctt tcttgttaaa cttatcaatt tctttattag ttttattaca aggagttatt
     9061 atatcaatac tagtgatttc tttatcatca aataaactag aaggaatggt aattacgaaa
     9121 ttctcaggtg tttttatgat gggtatttta gcaccatttt ttattttaaa taaagcacaa
     9181 tatattctgt tcttcttacc aactttttgg atatcgaagg catttaagga agataattat
     9241 atctatatgt ttatttcaat tgttatatca ttaatatgga ttttattatt atttaaaaag
     9301 tttagtaaaa aaatagttaa ataagaaaat ttaatatata aatgtaaggt tgacttgaca
     9361 gtaatgttga aaaatgttac atttaagatg taaagttaac cttacatgaa tggagcaaat
     9421 atgaaaaata aaataaagat attaagagaa aaattaggat tgacacaaga acaactagga
     9481 agattagttg gaacatctag acaggctata aatgctatag aaactggtaa aaatgaacct
     9541 tcaatatggt tggcatatga tatttcaaga atttttaatg aaccaattga aagtgtattt
     9601 ttatttgaag aaagtgaaag aaaatcaaga gcacagataa gtaggggaga atattatggc
     9661 tataagaaag ataagaacat ttgatgatga aattttaaga aaaaagagta agtatgtaga
     9721 aaacgtagat aataagataa gagaaatatt aaatgacatg gcagagacca tgtacaatac
     9781 tccaaatgga ggaggattag ctgcatgcca agtaggagta ttaaagcgat tagttgttat
     9841 agatttagga gaaggactta ttaagcttgt taatcctgaa attattaaac aagaagggga
     9901 acaaattgtt gttgaaggat gtttaagttt tccagaagta tggggtaaat taaaaagacc
     9961 aaaaaaagtt acagtgcaag ctctaaacga atacggtgag aaaatagaaa ttaaaggttc
    10021 aggttttatg gcaaaatgtc tttgtcatga gatagaccat ttaaatggta tagtttttac
    10081 agataaaatt attgaacatg taaaattgta agatagattg gttattgaat ttataaaata
    10141 ttttatagta aggagttaaa ttatgagatt atggcataag gatttaattg atgtattacc
    10201 taaaaatcaa ttagtaagtc aatggagaga gctacttgct ataaaaggtt ctatagataa
    10261 aaaaggtact ccaaaccatc tattagtgaa taaagtattg aatta
//
LOCUS       DAEPOG010000138        10275 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEPOG010000138 DAEPOG010000000
VERSION     DAEPOG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.138"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            100..1542
                     /locus_tag="KR432_002813"
     CDS             100..1542
                     /locus_tag="KR432_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0641332.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1584..1937
                     /locus_tag="KR432_002814"
     CDS             1584..1937
                     /locus_tag="KR432_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641333.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2128..2949
                     /locus_tag="KR432_002815"
     CDS             2128..2949
                     /locus_tag="KR432_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0641334.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
     gene            3207..5090
                     /locus_tag="KR432_002816"
     CDS             3207..5090
                     /locus_tag="KR432_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBH0641335.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5314..5994
                     /gene="pgmB"
                     /locus_tag="KR432_002817"
     CDS             5314..5994
                     /gene="pgmB"
                     /locus_tag="KR432_002817"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0641336.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            5991..8276
                     /locus_tag="KR432_002818"
     CDS             5991..8276
                     /locus_tag="KR432_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0641337.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            8502..9395
                     /locus_tag="KR432_002819"
     CDS             8502..9395
                     /locus_tag="KR432_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0641338.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            9443..>10275
                     /locus_tag="KR432_002820"
     CDS             9443..>10275
                     /locus_tag="KR432_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641339.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     L"
BASE COUNT         4198 a          954 c         1836 g         3287 t
ORIGIN      
        1 atatataatt attaaattat aataaatgac tatagcgtag atgtaatact atgttatagt
       61 catttttgaa ttaaacttga tatatcaagg gggaagaata tgagaacaat tagaagagtt
      121 ttagcattag gtttaacatt agctatattt ctaataaatg tacctaatgt agatgcatta
      181 acatcagata caattaaagg taacaatata tatgagacgg ctggattaat agcagataaa
      241 aagagttatg atacagcaat tatggtaaat atggataatt ctatagcaga tggtctttca
      301 gcaagtggtc ttgcaggtgc tgttgatgct ccaattttgc ttgcacaaaa aaataagata
      361 ccaaatgaaa caaaacaaag attaaaaaat gttaaaaaga tatacataat aggtaaagaa
      421 ttatcaataa gtaagtcagt agaaactgaa ctaaaaaaca caggggcaca ggtaactaga
      481 ttgggtggag atgacagaat aaagactagt tatagtgttg ctaaagaggt aaatggtatc
      541 aaaaaagttg atgaagtgat attaactaat gcatataaag gagaagcaga tacaataagt
      601 gctgcacccg tatcagtaag agatatagct cctatagtac ttacagatgg aaagagtgtg
      661 cctttttcaa caagcaatgt aaaaacatat gcagtaggtg ggagtatatc aatgagcact
      721 agtttagtta ataaaactaa tgctaaaaga cttggtggtt cagatagata tgatacaaat
      781 aagaaagtta taaaagagtt ttatccagat gcatcagaat tttatttgag tgatggatat
      841 gatttagtaa atgcacttac aggttctaca attgctaagg aaaatccaat tgtattggtg
      901 tcagaaagta gtgataagtc tatattagca ggagcagata agattactag attaggttca
      961 ataagtgaca gtgtgtataa taagtgtgtt tctgctgcac aaaataatgg cgattcgtct
     1021 acaaaaggtg agtcacctat gaaaaatgag acaagcatat taggtcagcc aacagctagt
     1081 ttagaagcgt gcttaaaatg ggcaaaatct aaaaaagcta atgatttatt tatagagtta
     1141 ataccaatat tatatgatac tgctgttcag gaaggtgtta atcctgtttt ggcagtagct
     1201 caatctgcaa aagaaactgg tttttgtaat tttggtggag tattagatgc atcatttaag
     1261 aatccttgtg gacttaaaac ttctgtaggt ggttctgata ctgataagaa tgcccattca
     1321 agatttgata cttgggaaga aggaatatta gctcaaattc aacatttatg cttatatgca
     1381 ggacaagatg gttacccact ttcaaatcca gtagacccta gacatgaaaa atctttattt
     1441 ggtaaggcta aaacagttga aagtttatct aataattggg ctggaggtca atatggacaa
     1501 gatttagtaa gaatgatggg agaaattgag gcaacaaaat aattgaagat tatcaaatat
     1561 ccaataaata aggattgatt attatgaaaa agagtaatat ttttagtatc atatttccta
     1621 caatcataat ggtgctaatg tttttaactt ttgctacaga tatatttaat attccagata
     1681 ttcaatctaa aagtattttt gttgtgggaa tggttttgat ttttccaatt tcttttttaa
     1741 tacaagggat aatttgtgta ttaaataaga ctaattggat tttatctctt attgtgtctt
     1801 tagtaactta tatagtatta atgtatatat ttttaaatga ttctgcatat atatatgttt
     1861 tactttatac agctttttat atgattggac atataacagc taaactctat tacaaaatta
     1921 agacttttaa aaattaaatt gaagtatata aaagtaacat taagagaagt atccttaatg
     1981 attttctaaa atcagaaata ggtgcttctc ttttttgtaa aaataaaagt atgtaaataa
     2041 aatttaacca ttataatttt cttttaacaa atgtatacaa taaacttgtt gtataattaa
     2101 gatagataaa ataaagggag ggataatatg aatttaatag ataatcatat tcatactaat
     2161 ttttcaagtg atggaaagga ttccatggaa gatacaataa aaaaagctat aagtattggt
     2221 gttagatatt tgacttttac agaccattta gaacatgatg aagatagagg attttgcata
     2281 aattataatg actatgttcc agtatttaat aagtttaaag aaaaatataa aaaggatata
     2341 gagttattac taggtgttga agtaggttac agaaaacatc taaaaaatga aatagaagaa
     2401 ataataaatt ctaatccttt tgattttgta ttgtgctcaa ctcatacaat tgacaatgta
     2461 cctgttccat caaaggcgta ttttaaaggt cttagcaaag aagatgcgta ttataaatat
     2521 tttaatagca tacttgaaac taatcgtgaa tttggagatt acaatatata tggtcatttg
     2581 gattatatat ctaggtatgg aatatattca gataatagag ttatatacaa tgactttaaa
     2641 gatgttattg atgaggtctt aaagtcaata atcaataatg ggagtggtat agagctaaat
     2701 acttctggtt atagatatgg attaaatgct atacatccaa atgaagatat tctaaaaaga
     2761 tataaagaac ttggaggatt tatagttact gtaggttctg attctcatag ggtagaagat
     2821 atatgtaaag attttgatgt ggcctatgat atgcttaagt atcttgactt taaatatgta
     2881 tctttattta aagaaagaga aacttatttt gtaaatataa aaaaagttaa atctaacaat
     2941 atagcttaga gtatgttttg aagtaaatta atggatagtt aaaaagttaa tagttataat
     3001 attgtatcct ctagaaaaat actagaggtt tttattttgc taaaataact aaatagaatt
     3061 aaatttaaat agtatagttt attttaaatt gaatagttta ttgttgatat attttactat
     3121 tatatatttt tattgtgata taatagtaaa atatattaac ctatctttag attatataaa
     3181 ttattataaa ggagggaaat tttaaaatgt ttaataaaaa taaagaaaat ataactttaa
     3241 gtaatcaaat caaaggaaca attcaaaata gtttaacaaa agaagctata ttacatatac
     3301 caatgagcaa ttatgcctat ggttatgata gagaaacact acatataaga cttagaacta
     3361 aaaaaaatga agcgaaaaga gtaattttac gaataggaga ccaatatgtg tgggataaag
     3421 gaggtgcagg aggaggaaat ttaaatgcat ctggagttgg ttggtctggt ggaacaaata
     3481 tagtaatgag taaagaagta gaaactgaat tatttgacta ttggatagct aagtgtaagc
     3541 ctttaaataa acgctcacga tatggtttta taatagaagg tcaagaagaa aaacttttat
     3601 ttacagaaaa aagaataata gagttgggaa atgaaaatga tgaaaaggaa ttatgtgaaa
     3661 taggtaattt ttttggatat ccatatttaa attatataga tatacctaaa gtgccaaatt
     3721 gggtgaaaga tactgtttgg tatcagatat ttccagatag atttgcaaat ggtgacccgt
     3781 ctataaaccc agaaggagta gataaatggg gagctattcc aaccagagat aattttacag
     3841 gtggagattt acaaggtgtg atagaacact tagattattt gagtgatttg ggaataaacg
     3901 gaatttactt ttgccctatt acaattggaa aaaccaatca tagatatgat acggtagatt
     3961 atatggaggt agaccctaca ttaggagata aagagacttt aaaaaaacta atagaagaag
     4021 cgcataagag aaatataaaa ataatgttgg atgctgtttt taatcatata ggatattatt
     4081 ctaaacagtg gcaagatgta gttcaaaata aggaaaattc tcgatataaa aactggtttt
     4141 atataaaaga tatgagtaaa attgatactc caatagaaca gatagatgaa aaaaatatac
     4201 cttatgaaac ctttgggtgt gaaaaatata tgcctaaact taatacagaa aattcagaag
     4261 taataaaata tttattggat gtaggcaagt actggataca ggaatttgat atagatgcgt
     4321 ggagattaga tgtttcaaat gaagtagacc atgttttttg gagaaagttt agagaagaag
     4381 tcaaaaaagt aaaaccagac atttatatat tgggagaaat ttggcatggt agtttaccat
     4441 ggctaatggg tgaccaattt gattcagtta tgaattactt gatgtcagaa gccatgaaaa
     4501 aattcttttg tacagatgag ataaacgccg aagaatttaa gtatatgata aatgatgtaa
     4561 tggtaagtta tccaatacaa gttagtgaag ttatttttaa tttacttgga agtcacgata
     4621 ctacaagaat attgacttat gctaatggaa atattgatag atttaaactt gcatatatgt
     4681 ttatgtttgt acaaacagga tgtccttgta tatactatgg tgatgaaatc ggaatggaag
     4741 gagaattaag ttcaatatct gaaggtcaaa gaaaatgtat ggaatggaat gaagaaaaat
     4801 ggaataagga tatatttgat tttatgaaga agattattag acttcgtaaa gaaaataaag
     4861 aacttaggtc tatatcaaat gaatgggtgt tagcagataa ggaaaatgga acgattatat
     4921 tgaaaaaaga atctatttct ataataataa ataattcatc taaaaatata attttgacgt
     4981 taccagatta tttagcaaat aaaaaagtaa aagatttata tgaagaagaa atagttgatt
     5041 taaaagaaga actaatgtta gagaaataca agttcattat tttaaaataa tacaaaaagt
     5101 atttatttta gatttcatgt taaattatta tacagtttaa acaagtcttt ataaaacata
     5161 aaaaaatatg gtttacaagt gagtgaattg aaagtagatt ttaaattaaa gactgagtta
     5221 gagtagataa ttttataaat aatatcttaa atacaagata gaaaataaat tgattattag
     5281 tctaaaaatt tcttgtagaa agaggaaata agaatgattg aagcatttat atttgattta
     5341 gatggtgtaa ttacagatac tgcatattat cactatatgg cttggagaaa actagcccat
     5401 aaagttggga tagatataga cacaaaattt aatgaatctt taaaaggcat aagtagaatg
     5461 gagtcattgg acaggatatt agaatttgga aataagaaat attcattttc agaagaagaa
     5521 aaagtgagaa tggctgaaga aaaaaataat tactatgtat cattgataga tgagataact
     5581 tcaaatgata ttttgccagg catagaaagc ttgttaattg atgtaaaatc taataatata
     5641 aagataggtc tatcttctgc aagtaagaat gcaataaatg tattaaatca tttgggtata
     5701 agtgacaaat ttgattttat agctgatgca ggaaaatgta aaaacaataa accacatcca
     5761 gagatatttc ttatgtcagc gaaagggctt aatgtaaatc cacaaaattg tatagggata
     5821 gaggatgcaa gtgcaggtat agacgctata aactcagcta atatgttttc tgttggtgtt
     5881 ggaaattatg aaaacttaaa aaaagcaaat ttagttgtag attcaacaaa tcaattaaag
     5941 tttgaatata tacaagaaaa gtataatgag tatatagtta ggagaattat atgaaatgtt
     6001 tatttagtaa tgtagatgag tggaagatta tacaagataa gattgaaatc aaagaaaata
     6061 gattagcaga gtccataatg agtataggca atggatatat gggtatgaga ggaaactatg
     6121 aggaaaatta tagtggagat acacacaagg ggtcttatat agctggagta tggtttccag
     6181 ataaaactcg tgttggttgg tggaaaaatg ggtatccaga atactttggt aaaattccta
     6241 attcagtcaa ctatataggt gtaagaattt tcatagatga tgtagaatta gatttagcaa
     6301 aatgtaaagt agaagatttt tatagagaat tagatatgaa aaatggtatt ttaaatcgta
     6361 ggtttattgt aaatataaat gataaaaaat ttgaagttag ggttactagg tttttaagta
     6421 tatctgttaa ggaacttgca gtaattaaat atagtataaa atcattaaat tctaatacta
     6481 aaataaaatt tgtaccatat ttagattcaa atataaaaaa tgaagattca aactatgagg
     6541 aaaagttttg gaatggaatt tcttcaagtt ctttagaata taaaggtagt attctttctc
     6601 gaacaaagga gaatgcattt aatacaccag tattcactgt gggttcaatg atgtatatta
     6661 agacaaatgg tgatgtagaa gctgtatctc atgagagtga ttataattac tgtgaaaatt
     6721 cgataacaat ggatgtttta aaaaataagg ttgcaagtat agaaaaatat gtatctataa
     6781 cttcaactag agattataag gaaagtgagc tattaagaaa atgtgaagac attttaaata
     6841 tagaactatc taaaagttat gatgatatat taaatgaaca tacaaagtta tggaataaaa
     6901 gatgggaaag tgcagatgta aaaataaatg gagataaaag tagccaacaa ggtataagat
     6961 ataatttatt ccaactattt tcaacatatt atggagaaga ttcgcgatta aatataggtc
     7021 caaaaggatt tactggtgaa aagtatggag gagctactta ttgggataca gaagcatttt
     7081 gtgttccagt atatctaggt gtagcagata aggaggtaac tagaaatctt ttagaatata
     7141 ggtacaatca gttagaacag gcaaaggata atgcaaaaaa actagggctt aaaggtgctt
     7201 tatatccaat ggtaacattt gatggagtag aatgtcataa tgaatgggag ataacatttg
     7261 aggaaatcca ccgaaatgga agtatagtat atgcaatata taactataca aattatacag
     7321 gtgactataa atatataaaa gaaaaaggaa tagatgtaat aatagaaata gctagatttt
     7381 gggcaagtag agttcatttg tctacaaaaa aagatttgta tatgatacat ggagtaacag
     7441 gtccaaacga gtatgaaaac aatatcaata ataattggta tactaattgt atagctaaat
     7501 ggtgtttaga ttatgctgta gaaaatatct tacgattaga gaaagagtgt aatgaatgta
     7561 ttgacagaaa taatgtgact agggaagaag ttgagttttg gaaaataata tcaaataaaa
     7621 tgtatctgcc gtatgatgag gaactacaaa taataattca aaatgataat tttttagata
     7681 aagaatttgt agaagtaaag gatttaccaa aagaaaattt tcctcttaat caaaattggt
     7741 cttgggataa gattcttagg tcgtgtttta taaaacaagc agatgtatta cagggaattt
     7801 actattttgg aaatagattt actaaagaag aaaaaaagag aaattttgac ttctatgaaa
     7861 aatatactgt ccatgaatct tctttatctt ctagtattta ttctattatt gcctcagaaa
     7921 tagataacct agaaaaggct tatgaattat actcaagaac agctaggctt gatttagata
     7981 attacaataa tgatacaaat gatggactgc acataacttc aatgtctggt tcatggcttt
     8041 ccattgtaca tggatttgca ggcatgagaa catggaatga aactctatct tttgacccta
     8101 agttgccaaa agagtggaaa aattattctt tcaatataaa ttatagagat aatattatta
     8161 atgtagatgt atgtagtgat ttaattaaaa tagaaaattt aaaaggtaaa ccaataaaaa
     8221 ttattgtata tgggaaaaaa tatatattag aaaaatatat aaaaattgat aagtagattt
     8281 aataatattt ttattatata aatagttaaa ataagtttat ttaattattt atctatttta
     8341 actattttgt gtgatagaaa actatatatt ataacaatag tagtgccttt aattaaatat
     8401 atttcataaa tcttcataaa atcttcaaaa tttcgtaaag aatatgtcac aaaagtaagg
     8461 tatattatat tcagcagata gtataaggga ggacaatatt tatgaagaat aaaaaattca
     8521 ttgttataat actgataata tcaattttaa taggtgtatt agtgaaggaa tattcatcta
     8581 gagagataaa aaaggatgat ataaatgtca gtaagtatat aaaatatgct gatttagcaa
     8641 gtaaaaataa tgcacaagtt aactggaagt atgtagcttc cattgttgct gtgttaaata
     8701 aaaataactt aaaaaacgtt aaggattcac agatacaaga agtatcagat ttatttgtta
     8761 agaatttttc taaaaataat aaaataaata agttaagtga tattttggat gagttagaat
     8821 ttagtaatag acaaaaaaga ttggttgata attatataga caatcttaag gattatggta
     8881 taaaaccaga aagattgaag tctgatacaa aatatatgaa gtttattgct gaaattaaaa
     8941 cagaagcaat acaaaattat aaggattata aaatacttcc atctataact attgcacaag
     9001 ctataataga atctagctgg ggcaagtcaa ctttggcaaa acaatataac aatctatttg
     9061 gtataaaggc tgacgcttat tggaaaggta agtcagtgac tttagaaacg aaagaacatt
     9121 tagatacaat tatagatgat aaatttagaa tatatgatga taaaaatgaa tctataaaag
     9181 accatgcaaa atttttagcc acaaataaaa ggtataaaaa caatggtgta tttgatgcaa
     9241 aaacgtatat atatcaagct aaagcattag aaaaggcagg ttatagtact gcgaaggatg
     9301 aaaatggcaa tagtatatat gcagctcgtc taatagaatt aattcaacag tataatcttc
     9361 aattaataga cagcgaaatt caaagtgaag tgtaactttt atgtaacata ggagtgatat
     9421 atttatcgag gaggtggttt ttatgaaaaa aatattctcg atatttataa gttgtatggt
     9481 tgttgtaact actattactg gatgtagtag tggtgaggtg ataaaaaata catcacctat
     9541 caatagtgtt aaaaatttgg attttagttt tgaggtgaaa cctgaaatat ttgaggtatc
     9601 agttgaatct ggtggttcaa aagaaaagat atctgaaccc tcaaaagaaa ggaaagtatc
     9661 aaattttaaa aagaataaga cagaggcatc gtggacgtat cctgatgaaa atatagacat
     9721 aaatataaaa aaagaggaca gctatttaga tgtaaatatt aaatctaatt tagaaaaaga
     9781 aactagtttt aattggccca aagttgaagg ggaaagttat atactaccac tttatgaggg
     9841 taagtttata ccaagtaatg ataagtattg gaaggagtat ctaaatgagt caacttataa
     9901 tgttatagaa tctttttcaa tgcaattctt tgcagtaaac aaggcaaagt attctgtgat
     9961 ttatattatc gaaaatgaat ttaacaatgt aattgaattt agttctaaag acaagattaa
    10021 ttttacattt aaacatgaat atccaagtat caataaaaat aaggagtatg ggtttaaaat
    10081 atatttgact aataagaata ttacagatgt agcaaaaact tataaaaatt atatagttga
    10141 taaaggagaa tttaaaacat taaaagaaaa atctaaggaa aataaaaata ttgaaaaact
    10201 ttatggagca ccacatattt attttggaga taagtctatc atcacaataa atgatattga
    10261 atggaataaa ttaaa
//
LOCUS       DAEPOG010000139        10241 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEPOG010000139 DAEPOG010000000
VERSION     DAEPOG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.139"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            400..1584
                     /locus_tag="KR432_002821"
     CDS             400..1584
                     /locus_tag="KR432_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641340.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1961..3070)
                     /locus_tag="KR432_002822"
     CDS             complement(1961..3070)
                     /locus_tag="KR432_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0641341.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3533..4726
                     /gene="nifV"
                     /locus_tag="KR432_002823"
     CDS             3533..4726
                     /gene="nifV"
                     /locus_tag="KR432_002823"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBH0641342.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4751..6676
                     /locus_tag="KR432_002824"
     CDS             4751..6676
                     /locus_tag="KR432_002824"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBH0641343.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6698..7693
                     /locus_tag="KR432_002825"
     CDS             6698..7693
                     /locus_tag="KR432_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBH0641344.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            8020..8493
                     /locus_tag="KR432_002826"
     CDS             8020..8493
                     /locus_tag="KR432_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0641345.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8527..9021
                     /locus_tag="KR432_002827"
     CDS             8527..9021
                     /locus_tag="KR432_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641346.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            9118..9639
                     /locus_tag="KR432_002828"
     CDS             9118..9639
                     /locus_tag="KR432_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0641347.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            9828..10139
                     /locus_tag="KR432_002829"
     CDS             9828..10139
                     /locus_tag="KR432_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0641348.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
BASE COUNT         3958 a         1132 c         1857 g         3294 t
ORIGIN      
        1 atcaaagtta cttacattaa agtatatttt ttcacaaaat atattgattt attcttttaa
       61 agtttgataa ttccaatgaa tgtaataaaa gaagggattt tatactatac aattattatt
      121 ataaaattat aaagaaaaaa ttaaataata ataaagaaaa aatgtataat atttaacatt
      181 aaaaaagata tttcataaaa ttaaaaaaca aagtgaattt gaatactatt taaaaaataa
      241 aaaaatatat aaaaaatatt taatacaaag gtagaaataa gttgaaaatg taaagtcatt
      301 ggaaaaatat agttgaattt aatgaacaaa aatgttattg ttttatcaat gaagtgttaa
      361 aataaaaact ttcaatatca ttttagggag gatgatttca tgcaaaaaag tgtaaagaaa
      421 gcaaaagtaa cgggatctat gttagccatt tttggtgttg caagcgttct ttttagttca
      481 catgcaggag gaggatttgc tacagggaat caagaaactc agtactatgt gcagtatgga
      541 tggacagcac cacttatggc aattttagca atgattatac ttactgctac catgagagaa
      601 gttatcataa tgtataataa taacaattgt aggaattata aggatttatt ttgtgagctt
      661 tggagaccat atccaaaact tgaaataata tgggagatat attactatct aatggtttta
      721 atagctgtaa gtgctgttat agcaggtgca gcagcagtat ttcaaagtat aggagtaaat
      781 tattttgtag ctgtatttat tattggagtt gtacttcttg tctttactat atttggtgct
      841 atgcttgtat ctaaagcagc tacagcaatg acaatagcta ttctagtatg tactcttact
      901 atttttattg ttggtataaa agctaaagta ccagaaataa ctgaaatatt atcaaataga
      961 acgagtttca cacctggcta tatcaagcca atacttaata cttttattta tgcaggtttt
     1021 caatcggttg ttatacctac acttgcagga tgttcaagac cacttcttaa gaactcaaaa
     1081 gaagctacta aggcaatgat aatttcattt gttatgaatg caattgcatt aggacttgct
     1141 gtaactatgc taatgggatg gtatagagaa attattgctg ctgggcaaac aacactccct
     1201 actctttatg ttgcaggtca atctggaaat catactatat acataattta taatgttgca
     1261 ttattccttt gtttaatgtc tacaggagta actactatat ttggtcttgt aaatagattt
     1321 gaagaccata aagcactttc tttcctttct tcaagaatga aaagaagggt atttacagct
     1381 tgtgcaataa tggtagtgtc aatgcttata tcacttacag gattaagtaa tattgttaaa
     1441 tatggatatg gatattgtgg ttatcttggt ctattcacta tagtaattcc attccttaca
     1501 ctaggacatt ataagaataa gaagtttgca aaggaaaatc cagaagctaa atggccagcg
     1561 gaattaaatg aaaatgtaaa ttagatgtag tagcaaaaaa tattgctaaa gagaatacta
     1621 taaataagaa tactatagat aatatatctg catgtttatt aagattattt gcaaaatcta
     1681 atattgaaca actataaaca agcaatataa aactaagaat aaaagataaa aagagggtgt
     1741 ctcaaaatgg acttttagtt tataaggcac tcttttttat atgaagtcaa atatattttc
     1801 attattttga caataaaaga gactaatcct actttggata gcctcttttt tctttaatat
     1861 aaaatagtat aacagcaaag tatagattga tacatgtttt ctgtagcttt ctatatatta
     1921 cagtgtttgt gtggcattgg tattttaagc aatctataaa ttataattct ataattcttc
     1981 tactaatttt agattctata gccgtctcta tttcacttaa cattcgttca tttttagggt
     2041 caataaaagc acatgtatag ccttcttcac cagctctacc agttctacca atacggtgga
     2101 tgtagtcttc tggtgtttct ggtaaatcgt aattatatat atgacttact ccagatatat
     2161 caaggccacg agaagctaca tctgtagcta ttaagtattg tatatctaaa tttctaaatg
     2221 atttcattat tctttctcgt tttgattgag caatatcact atgaagtttt tgacaattat
     2281 atcctcttat agcaagagct tcttcaagat tgtcaaccct tctcttagtc ctacaaaata
     2341 ttattgccat aaaaggatta tcttcatcta gtaccttaca taaggcatct aatttttttc
     2401 tatctgtagt ttctactact tcttgtttta tattattaag agtgatttct tctttttgaa
     2461 tagaaactac aacaggattt tttgtgtatc tataagctaa tttttttact tgagaattta
     2521 tagttgcaga aaagcatagt gtttgctttt tgtttgaagt ctcttttaaa atagcttcca
     2581 cttcgttttt aaatcccata agcaacattt ggtctgcttc atctaaaaca aaagtcttta
     2641 acttactaag gtctattgtt tttctattta gatggtctaa aagcctacct ggtgtagcta
     2701 taatcatatg aatatttcct tttagtttct ttatttgaga accaatatct tttcctccat
     2761 atgctgcaag aatacttaca tctttagatt ctttaagttt catagcttct tctgttattt
     2821 gaatagctag ttctcttgta ggagaaagta taagtacttg tatatcattt atatctaatg
     2881 atatattttc aaatatcgga agtaaaaaag ctagtgtttt tcctgttcca gtttgagctt
     2941 ctgctattac atcttttcct tcttttatta gtttaatact ttctttttgt ataggtgtag
     3001 gtgttgttat tcctgatttt tttagaatat ttattgtatt ttcactaatc cctaagcttt
     3061 tgaaatccat tgttatctta cctttcatga aaaattttct tttttattaa ccaattaata
     3121 atatcatatt tgtaagtatt tttagtaaat tttttaatta ttatttatat agcttaaaaa
     3181 tatattaaga tactttaaaa tggaatgtaa cttaacttaa attttagtag gaattttata
     3241 ggtttatttt taaaggtttt ttgaaaataa atctatttaa aaattaaatt aaaatgcaag
     3301 aaaaacataa taactttcat ttttaagaag ttttcacaaa aaagaaaaaa tatagtgcaa
     3361 taaatgatac aaagtaatat aatagattac aagatatgaa aagctaaaaa aataaattgc
     3421 aaatgaaatt aaaatgaata aaaaataaaa aaataatcat aagttagagg tatcaatgaa
     3481 aaataagact taatgttttt aaaattaaaa gtatactaag gggggcatta atatgtgcgt
     3541 tatatcaaaa gatagagcaa aggaaataaa aatagtagat actactttaa gagatggaga
     3601 gcaaacagct ggggttgtat ttgcaaatag agaaaaaata atgattgctg aaatgttaag
     3661 tgacctaggt gttgaccaaa ttgaagttgg tatacctact atgggtggag atgaaaaaaa
     3721 tgtaataaag cacatatgta gtagaaattt aaaatcagat ataatggctt ggaatagagc
     3781 agtaataaaa gatgttgaag aatctatttc ttgtggtgtt gatgcagtag ctatatcaat
     3841 atcagtatct gacattcata tagaaaataa attgagaact tctagaggct gggttttaga
     3901 aaatatggct aaaacagttg aatttgcaaa gaaaaatgga ttatatgttt cagtaaatgg
     3961 agaagatgct tcaagagcag atatagactt cttaactgaa tttataaatg ttggaaaaca
     4021 agcaggggca gatagattta gatactgtga tacagtagga gttatgaatc ctttttctat
     4081 aaagaatgca atagaaactc tttatgaaag aactaatttt gatatagaaa tgcatacaca
     4141 taatgacttt gggatggcaa ctgcaaatgc acttgcagga atagcagcag gagcaaatta
     4201 tgttggtgtt acagtaaatg gattaggtga aagagcagga aatgcagcat tagaagaagt
     4261 tttaatggca cttaagtgtg tttataaatg tgatttaaat aatatagata ctagaaaatt
     4321 tagaggaata tgtgagtatg tagcacaggc atcaggaaga atacttccta catggaaacc
     4381 agtagttggg gataatatgt ttatacatga atctggtata catgcagatg gagctttaaa
     4441 agacccgcat aactatgaac catttgaccc aagtgaagta aatcttgaaa gaaaaatagt
     4501 aataggtaaa cattcaggaa gagctgcagt tataaataag ttgagtgaat atgaaatgta
     4561 tatttctcca gaaaacgcta ccaagctatt aaatgctata agagctacat ctataagatt
     4621 aaaaagaagt ttaatggata aagaaattct tcaattatat tgtgatatac tagcacatga
     4681 gaaaggcaca acagaagaag aagcagttag aggttcatat atttaagata taattgttta
     4741 ggaggaaaat atgggagata atatagttta taaaataatt aaaaaacata tagtagatgg
     4801 agaagctgtt gctggaagtt caattggtat aaaaatagac caaactttaa cacaagattc
     4861 tactggaaca atgacatatc ttcaactaga agctatggga atagataaag taaagactaa
     4921 aagaagtgtg gcatttgtag accataatat gttacaacaa ggatttgaaa atgcagacga
     4981 ccataagtat atacaaacag tggcagataa atatggagtt tatttctcta agccaggaaa
     5041 tggtatttgt catcaagtat ttttagaaag attttcaaca ccaggagata cacttttagg
     5101 ttctgatagc cacacaccta ctgctggagg tgtgggcatg atggctattg gagctggtgg
     5161 attagatgtt gctttagcca tggcaggagg agcatactat ataaaagcac caaaagtctg
     5221 taaggtgaat ctagttggaa aattaaataa tatggtatca tcaaaagata taatattaga
     5281 agtgttaaga aaacaaactg taaaaggtgg agtaggtaaa gtttatgaat atggtggaga
     5341 aggtgtcaaa agcctatctg ttccacaaag agctacaata acaaatatgg gggctgaact
     5401 tggagcaact acttcaatct tcccaagtga tgaaaaaaca ctagaatttt ttaagagtca
     5461 aggaagagaa gacgcatgga tagagttaaa accagatgca gatgcagttt atgatgaaga
     5521 gattactata aacttggatg aattaaaacc attagctgca aaacctcata gcccagacaa
     5581 tgtagatgaa gttgaaaata taggcaagat taaaatagac caagtggcaa taggaagttg
     5641 tacaaattct tcttatgaag acttaatgaa agtagcacaa atcctgaaag gaaataaagt
     5701 acataaggat gtgagtttgg taatagcacc aggttcaaga caagttatgg agatgattgc
     5761 tagaaatggg gctttggcag atataataag tgctggagct agaatactag aaaattcttg
     5821 tggtccatgt attggtatgg gtcaatctcc aggtactgac tctgtgtctt taagaacttt
     5881 caatagaaat ttctatggta gaagtgggac tttgtcagca caagtatatt tagtaagtcc
     5941 agaagtggca gcagtaagtg caatcaaggg agtgcttaca gacccaagag aatttgatat
     6001 taaatttaca aatttagatg taaatgaatt tttgatagat gatagcatga taattaaacc
     6061 tgctgatgta ggaagtaatg tagaagtagt tagaggacct aatataaaac ctttccctct
     6121 taatacagag ctaagtcagt ctataggtgg aaaagttata ttaaagacag aagataatat
     6181 aactactgac catataatgc catcaaatgc taagttactt ccttttagaa gtaatatacc
     6241 gtatcttgca aactattgtt tcaatactgt agatacagaa tttcctcaga gagcaaaaga
     6301 caataatggt ggatttatag ttggtggaga taattatggt caaggttcaa gtagagaaca
     6361 tgcagcttta gcaccattat atctaggtgt taaaggggta atagttaaga gctttgcaag
     6421 aatccacaag gctaacttga taaatagtgg tataattcca atggagtttt gtgatgagaa
     6481 agattatgaa aatatatctc ttttagataa tctggagata ccaaatattt tagacaatct
     6541 aggtagtgga attttagaag ttaagaatac aactaaagga acttctttta aggtcaaagt
     6601 agaattgtca gcaaaagaag ttgacgtatt aaaagctggt ggaaaattaa actacactaa
     6661 aaatcaagct aattaatatg aaaagaggga atattgaatg tataaagtga cacttatacc
     6721 aggagatgga ataggaccag aagttgcgaa agctatgaaa aaggtagtag aagctactgg
     6781 agcagaaata gaatgggaag aagttaatgc aggagaggca gttattgaag agtatggtac
     6841 tccacttcct gaatatatta tagatagtat aaaaaagaac aaaatagcta taaaaggccc
     6901 aattactact cctgtaggta aaggatttag gagtgtaaat gtaactttaa gacaggcatt
     6961 agatttgtat gtcaatttaa gacctattaa aagttttaag gggataaaat caagatatga
     7021 agatgttgat ttagtggttg taagagaaaa tacagaagat ttatatgctg gaatagaaca
     7081 taaaataggt gattatgcag ctgaaagtat aaagataata acaagaagtg caagtgaaag
     7141 aatcgttgat tttgcatgta actatgttaa agataataag agaaaaaaag ttacagctat
     7201 acataaggca aatataatga aaatgtctga tggattgttt ttagatgtgt ttagagaagt
     7261 tgcatcaaaa catggtgtag aatatgatga tttgattgta gatgcagcag ctatgaactt
     7321 agttttaaat ccagaaaatt atgatgtaat ggtaatgcca aatctttatg gagatatact
     7381 atcagattta ggtgctgggc ttgttggagg tttaggtata attccaagcg caaatattgg
     7441 taaagattgt gcgatatttg aagctgttca tggaagtgct ccacaaatag caggtcaaaa
     7501 taaggctaat ccaacagcac ttattcaatc atcagtaatg atgcttagat acttaggtga
     7561 atatgaaaat gcacaaaaaa tagaaacagc tttagaaaaa gtatttcttg aaggaagtaa
     7621 attgactgta gatttaggtg gctcagcttc tactacagag tttgcggatg aggtgtgtaa
     7681 gtatattgta tagattttat ttagggaaaa agtgaaatat agattgaagg aaagaggctt
     7741 tctcaaaata gagataagcc tttttttatt ggaatgataa aatatatttg attttataca
     7801 aaagagagtt cctcataaac gaaaaagtta attttgggat atccatttta attttaaaat
     7861 taattgatta atacgcaaag aaagattaaa caaatttatt atttttaatt gaactttcaa
     7921 ccaaaatatg gtaaaattta aaaaagttga actttcaact aaaaataaat atattgatta
     7981 tgtgaaattc tataaaacag aaactatttg actttagtca tggtaggttc agtggaggtg
     8041 tgtttgatta agactttaga tagtaatata ttaagagaag taggtacttt atctagagca
     8101 gtaaattcaa taaatgatat taagtacaag gaattaaagt tacaaaaagg acaatttaca
     8161 tttttgacaa gaatctgtga aaatcctggt ataaatcttg tggaattatc aaatatgtta
     8221 aaggtagaca aggctacaac aaccaaggct atacaaaaac taataaaggc aggatatgta
     8281 gataaaaagc aggatgagtt tgataaaaga ggatataatt taactccaac tgataaatct
     8341 ttggaagttt atgagttgat aattgaagaa gagaatagaa gtatagagat atgttttgat
     8401 aattttacag atgaagaaaa acaagtggta actaagttat tagaaaaaat gagcaaaaac
     8461 gtagaaaatg aatggtttaa agtaaagaga tagtaaataa atacattaac ttaaggagat
     8521 atttttatga ttagaaaagc gaatatgaat gatttagaaa gcattatgaa aattataaaa
     8581 tctacagtgg aagaaatgaa gacttataat aatacacaat gggatgaaaa ctatccatta
     8641 gaaaaagact ttgtttcaga tattaagaaa caagatttat atatttatga agtggatgga
     8701 gaagtagcag gatttatatg tcttaattat gaagagccag aagaatattt aggcttaaat
     8761 tggtcatcta ataaaaaagc aatggtaata catagaatgg ctgtaaatcc aaactttaga
     8821 aagacaggaa tagcatctaa gttagttgat tttgctgaaa aattagctgt agaaaacaat
     8881 gtatcatact taaagagcga tacatattca ataaactcaa aaatgaattt attacttaca
     8941 aagtgtggtt ttataaaaat cggagaaatg agttttttag gaaaggagaa atctttctac
     9001 tgttatgata aaatattata aagagcattt ataatacaag gggtaaataa tagattttca
     9061 gcattagtaa gggcataata taacccagta tatgctatta tgaaagggat gatttatatg
     9121 attggatttc taaagaaaag aagaagtatt agaaaataca aggatgttga agtagaaaaa
     9181 gaaaaattag ataaaatatt aaaagctgct cttttagctc catcatcaaa ggggcttaga
     9241 acatgggaat ttatagtggt agatgataaa gaaaagctaa ttaatttatc tcaatgtaga
     9301 actaagggtg gtggattttt cttaaaaaat gcacctttag ctattgtaat tattgcagat
     9361 aaagaaaaaa atgatgtttg gatagaggat gcaagtatag ctgcttcata catacaactt
     9421 caagctcatg aactaggttt aggttcttgt tggatacaag taagaaatag aatgtatgat
     9481 gataatatag aagctgataa gtacattaga gaagaattaa aagtacctag caagtacagt
     9541 gtagaatgta taatttctat aggatattct gatgaagaaa agaaagcata caatgactct
     9601 gatttagact ataaaaaggt tcattttaac aacttttagt atataatatg ttgtattaag
     9661 acattatatt tttttctatt gttaatatat acttaaaatt gtaaaaatat atctgaattt
     9721 tactaactta tctaggggtt atgtacaagg attttaaaga taaatataga aattaatgtt
     9781 tataatcgat acataacttg agtaacaaaa tttaaggagg ttaaagtatg ggtatattta
     9841 aaacaaaaat ggatgaggac tggaaagtaa actacattaa agagtttaat gaaatgagag
     9901 attcttatga gagtaaactt caaaaaaaac agtttgaggt agatagtttg aaatcagaat
     9961 tggatagact aagaagctat aaaaatagcc ttaagccaaa agaaaaacaa attactgatg
    10021 atgatataaa taatataaag agtttaagaa gagatggctt aagctataaa gaaatttcaa
    10081 atcaaacaag ttggagcaaa gcaactgtaa gtagggtatt aaatggactt tacgattagc
    10141 atatacatgg ggtgatttaa attgggatga agtatagtta caaaaaatgc ataatagatt
    10201 ctattttatt gatgtttata attcaaatat taaggatgat t
//
LOCUS       DAEPOG010000140        10103 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEPOG010000140 DAEPOG010000000
VERSION     DAEPOG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.140"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            248..898
                     /locus_tag="KR432_002830"
     CDS             248..898
                     /locus_tag="KR432_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0641349.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            1176..3242
                     /locus_tag="KR432_002831"
     CDS             1176..3242
                     /locus_tag="KR432_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0641350.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            3259..5214
                     /locus_tag="KR432_002832"
     CDS             3259..5214
                     /locus_tag="KR432_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0641351.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            5230..6420
                     /locus_tag="KR432_002833"
     CDS             5230..6420
                     /locus_tag="KR432_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0641352.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            7554..7928
                     /locus_tag="KR432_002834"
     CDS             7554..7928
                     /locus_tag="KR432_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0641353.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            7930..8787
                     /locus_tag="KR432_002835"
     CDS             7930..8787
                     /locus_tag="KR432_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641354.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            8790..9428
                     /locus_tag="KR432_002836"
     CDS             8790..9428
                     /locus_tag="KR432_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0641355.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
BASE COUNT         3722 a         1174 c         1804 g         3403 t
ORIGIN      
        1 aacgatttaa tctatagtac aataacttga tttgaagtac aacgatttaa tctatagtac
       61 aataacttga tttgcaatac aatgatttaa tctatagtat aatagcatga tttacagtac
      121 aataatttaa attgtagtac aaaagtacta tcaaataaga atttctactt taaaaatatt
      181 actaaaagta attaaaatcc aataattcaa tataatatgg caaaagaata gaaataaggt
      241 gagagtagtg aaagtaaagt caaatttaga tgaaatttta tatcataaga ttattgaaag
      301 tcttattcgt ggtgaatact ctgtaggtca aaaaatatta ctaaatgacc tttgtgaaaa
      361 atttgaagta agcagaacac cagttgtaca agcagtaaaa atgctaaata aagatggagt
      421 gcttactatt atgacaaatg gtaaagtgta cgtaccagag tatgaatatg atatggtaaa
      481 acaagtttgt gaaacgagaa cactgattga aacatatgct ttagaaaaaa tgatgcaaga
      541 agaggaagag gtttttcaac aaaagctaga tgtaataaaa agatactctg ataaatgcga
      601 ggagtactat cgtcaagaaa aatctgtaga acttgcacta gcagatttgg aattacataa
      661 agccattgta gaaggggcaa ataatgagat tttaaatgat gtatatgttg gtatacaagg
      721 aagatttata gtagtgaact atttaattcg accattaaaa aatcgtaatt atgaaggaac
      781 agtacaagac cattttgaaa tattaaaatt cattgaagac agagatacaa aaaaagcagt
      841 agataaacta agaaatcata ttcaaggaac tattaaaaga ttctgtgaag atgaataata
      901 agttttgaag ttactatata cttatctatt aagttgctaa atgtagtcta agtataatct
      961 aattatagtt taaacataat ctaaatataa tctaaatata gtgtaaataa gtctaagtat
     1021 gaattaaata tagattaagt atgaattaag catggattaa atatacttta aattacaaat
     1081 attacaagga agccagaagg cttcctaaaa aaataacctt tataaaattt tttattttaa
     1141 atttaaaatt ttttatttta aatttaaaat aaaaaatgaa agaggtggta gagagtttga
     1201 aaaaaattac taaagaaagg atagaaaata ctatgaaaaa gctatttacg agcgaaacat
     1261 ttttggaata tcaatttata agtgattgcc aaatatcacc agatggagga tatacagcat
     1321 ttattgtaaa aaaagctgat atcaaagaga atggttacac aagccaagtt tatatattaa
     1381 acaataaaac tggcgaatta aaacaaataa cgtctataaa ctctgttgga gcatatgtct
     1441 gggaagatga aaatactatc ttgttccctg ctttgcgaaa tgaaaaagta aaagaagctg
     1501 tcaaaaacgg taagcaatgc atgagttact atgctttatc tttaaatggt ggagaagcag
     1561 aagaactttt tagactccct atcaaaggtg gtaaattgaa tcctatagga aaaggtttgt
     1621 atgcagttat tgattcttat gacaatgacc gacctattgt agagggattg ccagaagaag
     1681 aacaacagaa aaaaataaat gcttataaca aaagacatta tgaacatttc aaagaaattc
     1741 cttatgctgt caatggggag ggatatatta gcagaaaaag aaaacgccta tatatatacg
     1801 acagtaatat aaataagttg aaggcaatta cagcacctat gtttaatgtt gtaggtatga
     1861 aaataagtga tggaaaaatc ttatatatag ggcaagagtt taaagatgta aaaggtttga
     1921 aaaatggggt ctatgtattt gatacaaaca caaacacaaa tatctgtata ttagaaaaag
     1981 ataagtatat tataaaaggt tttgaattat atcaaaatca agtgatacta aacctaactg
     2041 atgctttatc ttatggaaat ggagaaaatg gagattttta tactattgat attgatacga
     2101 aagaaatgaa acttctttca gggcatcaac atcattgtat tggaaataca gtaacatctg
     2161 atgttaaaat gggtgctggt cagacaacaa aggtagatgg agaatatatt tattacactt
     2221 ctacagtaga tatggactgc attattgaaa gaatacatat tccaacagga aaacaggaaa
     2281 aagtaacaca gacaggttca gttgatttta tagatgtaaa agatggcaat attgtatgtg
     2341 tgggttgttt aggaaatgga ttaccagaag tttatacagt agagaatgga acattgtgta
     2401 agaaaacaca tttaaatgac catattttag aggaatataa aatttctgtg ccagaataca
     2461 tagagtcaaa aggaagttct aaatgggaaa tacaaggata cgtatataaa ccagtagatt
     2521 atgaggtggg gaaaaaatat ccagctgtat tggcgattca tggaggacca agattgacat
     2581 atggaccata ttttatgcat gagatacagg tatttacaag tgcaggttac tttgtattct
     2641 tctgtaaccc aagggggtct gaaggtcgtg gcaatgcttt tgcagatatt aggaaacagt
     2701 ttggagatat tgattatata gattttatgg aatttacaga tactgtactt gaaaaatata
     2761 cagacattga taaaacaaaa ttggctgttg aaggtggaag ttatggtgga tttatgacga
     2821 attggataat tggtcataca aaccgttttg ctgttgcttg tgctcagcgt tctattgcaa
     2881 actggagtag catggagggt acaacagaca ttggttatta tttctgcaaa ggacaaacag
     2941 gtgcttctca tatggaaaac cacgagcttc aatggaaaca gtcacctctt gcttatgctg
     3001 ataaatgtgt tacaccgact ttatttttac atggtgaaaa agactataga tgctatatgc
     3061 aagaagcatt ccagatgttt tcagcattga aaattcatgg gtgtcctaca aaactttgtc
     3121 tatttgaagg ggaaaaccat gaactgtctc gttctggtag accaaaacaa aagctacaaa
     3181 gattagttga aatgctagat tggttttctg tgtacataaa aaaagataac ataggacagt
     3241 agaaaggaga atataaaaat ggaaaaaata aagattgaaa gttcagatat ttttgaatat
     3301 atattccctc atgatatttc ttgttcacct gatggaaagc atattgctta tattatatca
     3361 aatatcaacg aggaaaaaga ctgctatgaa catgacttat atgtgatgga catcaaaaca
     3421 gaaaaacaaa tacatatgac tcaaacaaaa gatgtaacat catttagttg gatttctaat
     3481 acagaactac tttttacatc taaaagaaat aagccaaaag caggtacaac agatttttac
     3541 actatttcta tagaaggagg agaagcaaaa gaagcatttt ctattccaaa agcttgtagc
     3601 gtacctgtat cacttggaaa taaattgtgg ttattgacaa caaaaaatcc aacggattct
     3661 aaaaaatcag aaccagatag ggcagtagag ggtgtagatt attggacatt tacagataaa
     3721 ccttttataa gagatagaga aaatttttct caaagaagac gtgtaacatt agaattatat
     3781 caagaaggtg aaaatataac aaaagccatt acaccaaaat tttgtgaagt agcaggtata
     3841 gacgtttctt ctgataaaaa cagaatttta tatactggtc agatttatga agattgtgca
     3901 actccttttt ctggactttg ggagtatcat atagacagtg gtgaaaccaa agaacttgta
     3961 ccacaaggaa agtaccaaat aagtttggca aaatatattg gcaaagataa agtaatgttg
     4021 caagcttcaa cattagaacg ttctatcaca cagaatcatg atatttttat acttgatttg
     4081 agtactagtg aaataaatat gattgctagt ccagatggta tgtatgctac attactggat
     4141 gtagatgcag tatatggtgg tggacgtagt aataaagtta taggagataa atttattggt
     4201 gcaagaattt gcagaactat gacagaattt aatgagtttg atacaaagac aggtaatata
     4261 cgtatcatca caaaagtaga tgcttttaca tcttttgata tttacgacaa caccatgtac
     4321 acagtaatgt tgaaagatta tgagttagca gaaatatata gcattgatat gactacaggt
     4381 acaatgaaaa aaatgactgc tttttctaaa ccatatttag atactcataa ggtttcatta
     4441 cctgaaaaat tgacgtttgt agcaaaaaat aaagaagaag ttgatggttt tgttatacca
     4501 cctatagatg ctaaagaagg cgaaaaatat cctgctgtat tgttcattca tggtggacct
     4561 aaatgggcat atggctatat gtttacacac ttgaagcagt gtgtaacatc aaagggaatg
     4621 tatgtgattt attgtaaccc tcatggtggt gacggttatg gtgaaaaatt cttagaaatg
     4681 gtagaaagat gggggtatgt tgactatgag catcttatgg aatttgtaga tacttgtatt
     4741 gaaaaatatc ctgggattga tgcagaccgc ttaggggttg cagggggaag ttacggtggt
     4801 tatatgacaa actggataat tggccataca gaccgtttca aagcagcagt ttctcaaaga
     4861 ggtattagta acttgattac agcttcttta attattgact ttggggatag aattatgaag
     4921 cagacttgtg gagataagac gccttggaat catgaggagg ttctttggaa tcattcacct
     4981 attaaatatg tgaaaaatgt taagacacca acattgttct tacatagtga tagggattat
     5041 agatgtttta tgggggatac tttccaaatg tttacagcat taaaacagct tggtgtagat
     5101 acagaaatgt atttgttcca tggagataca catggattgt ctagaaatgg aagaccatct
     5161 aatagaattg cacgagcaaa tgctattgta gattggtttg aaagatattt ataataaggg
     5221 ggttattcta tgagtgatat gttattatgg tttagtgtcg tattagtttt atttgctatt
     5281 ggggatttaa ttgcaagcaa aacaaaagca aaagtatcag ctgtatttgt tacactactt
     5341 ttatttttga ttctatttgt cacaaaagca ataccagctg atattattga aaaagcaggt
     5401 atgaccgcag cagctagttg gagtgttcca atgattatgt ttagtatggg aacaatgttg
     5461 aatgttaaac aatttataga cgaatggaga acagtattga cagcatggct tggaattgta
     5521 gcagttatag tctgtgtctc attatgtatt cctttgtttg gaaaatcaac agtattgaca
     5581 agcataccag ttataaatgg agctttacct gctactacta ttatgacaca agcagcacta
     5641 gaaaaaggac tgacacttgc agcagctaca gcaacagttg tatttgcaat tcagaagttt
     5701 attggtacac caatagcttc aagagcagcg ttacaagagg caaaccgtct tttagtagaa
     5761 tatcatgaag caaaatcaaa aggcattgat ttagcaaatg ttgatactga taagaaagaa
     5821 gcagagaata caggcacaaa agtaaaacaa gctttctgtg aaaaatacga taaatattat
     5881 tctacaaatg tttgtatatt ttttatagca ctattctctt atctagggta tgaactttca
     5941 gagattattc atgtaaatta ttctattgta tgtcttgttg ttggtgttat tgtaacacgt
     6001 attggtattg taccaaaaga cattttggaa aaaggaaaaa tcaagggatt tattaacatg
     6061 gttgtgtttg cagctgttat tccttcttta gcaaaagttt ctttgacaga tttaattagt
     6121 ctttttgtac caattgtggg tatgtttgca gcttctatca ttggtatatt ccttatgatg
     6181 aaggtgttac caggttggaa aattatcggt tctaaacctc ttgcttttgg agtagggttt
     6241 tgtcagatgt tgggattccc aactacatat ttaatttcaa atgaagtgtg taatgctgtt
     6301 ggagaaacgg aagaagaaag agcatatttg atgtctaaga ttatgcctaa gctggtagtt
     6361 ggtggtatgg cttgtatgat ttctattgta gtagctggtc ttatggtacc aatgttgtag
     6421 ataggtttca tagttaaaaa gtggtgttaa tttataggag aagctaaaaa gtagtattaa
     6481 tttataaaat aattttatat tagttaattt gttataagaa tattttcttt ttatgattat
     6541 aattttgatt ttataagaga gcagattttt tatttgctat gctaatagtt ataatttgtt
     6601 tttatttgct aaaatgcaac tttaatttga tttatgtata atatagggta tttaataata
     6661 tttttgaaga atttttataa aaaattaaaa agaggagaaa aaagggagtg ccacaaagtg
     6721 aactttttag ttctaaggca ctcttttttg tacgaaatcg aatatatttt caacgtttta
     6781 aaagtgagaa agagacttat ctctagtttg ggatagccaa atttaattat taaaattatt
     6841 ttttgtttta tttatcaaag agtattcgtg ttttagaagt tagtattttt tattttatat
     6901 atccaaggtt ttccgtttct aatgttttct tttttaaaat aattagggta ttcaattata
     6961 gtaaaaggtg gatggtattt tggaatgata ttatttaaag cacagtattg aatgttgaaa
     7021 tcctcacatt ttagagtatt atcttttgta atcaaaaaaa tcttctatac ttatagcaag
     7081 gttacttctg caaatattcc atgcatcata atatccaaat ttaaattttt ttaatataga
     7141 gtttacctct agtctacact cttgaataac tctttcctct atccaaattc ttataaactc
     7201 atcccattta ttactatcta tttattacta tctaaatttt ttagtatgtt tgtaattgga
     7261 aatggattta ttcctttatg ttttttataa atagtttata acttaattta ttattaaaca
     7321 aatcattcat acagaattac tttttctttt agatattcaa ttcttacttt taataaatat
     7381 ttaatacatt ctatcttatg tggttttaaa aagtgtttat ggctatcaat aatctctaat
     7441 atttcattca tatttatttt gtcatagtaa tattttacat cttttatatt gacaaaaaag
     7501 tgctctaagt gtatagtgtg tatataataa catcacactg gaggtgataa aaaatgaaag
     7561 tgctcatttc taacacatca gataagccat tataccaaca aattaaagag caaattaaag
     7621 aagcaatatt tactgaagaa ctgaaagaag gggatgcaat gccctcaata agaaattttg
     7681 ctaatgattt aaaagttagt attttgacta ttagacgagt gtatgaagaa cttgaaaatg
     7741 aaggttttct tacacgccaa gttggaatag gaacatttgt ttcaactgga aatttagaaa
     7801 tactaagaga ctcaaagagg cgtatagttg aacaaaaaat ggcagatatg attaaggatg
     7861 caaaagcact aggaattaca cagatagagt tacaagaaat gatgggtatt ctatatgagg
     7921 aggattaata tggaaaatat tttagaagtt tataacttaa ataagactta taccaatttt
     7981 tcattatcta atataagctt tgtattacca gaagattgta taactggatt tataggtgtt
     8041 aatggagcag gaaaaacaac aacaattcgt tcgatattgg atgtcgtaaa gcgtgatagt
     8101 ggaaaaataa aaatctttgg taaagatttc gatggaaatg agaaggaatt aaaaaatcga
     8161 attggagttg tatttgatga ttcatatttc tatgaggagt tgtcaatgtc agaaatgaaa
     8221 agtattattg caccttcata tgataattgg tgtgaagaag attatcaaca aaatatgaag
     8281 cgttttaatc ttaatccaaa acaaaagatt gatacacttt caaaaggaat gaaaatgaaa
     8341 tttgctttag cattagcatt atcgcataaa gctgagtttt taattatgga tgagcctaca
     8401 agtggtttag acccactaat ccgtagtcag atacttgaca tactaacaga atatatgaag
     8461 aaatgtggtc gtggtgtttt cttttctact catataacat ctgatttaga caaaatagca
     8521 gatatgattg ttatgattga caatggacgt attatattag agcgtgaaaa agatactcta
     8581 ttggatgaac atagaaaagt aaaaggggat attcaatttc taaatgatga aatcaaaaaa
     8641 ttatttttaa atttagaaat tacaggttat ggttttatag ggattacctc aaaaattaat
     8701 gaagtgcaaa aaaatatgcc agatatttta atagagcgtc ctacaattga agatattatg
     8761 cttgcatata ttgaaaggag agagtaatta tgttgattca tttagttaaa aaagatattc
     8821 tgattgtaaa aaaatatgta ttgttgatat tgtttattgc aataggaatc ccattattta
     8881 tattatggcg tttacctcca tttgcacaaa tattgggatt tgtactatca attatatttt
     8941 cagaatttat gctgtgccag tatctattat taaaggagag tcaatattca aaagcatctg
     9001 tattactttg ttcaactcct tatcctagac gtggcttagt tcaatctaag tatgttttat
     9061 ttgcaatggt atttatctat tgtacattag tttactggat tgaaaattta cttattccac
     9121 aaataggaac ttttgactta acatatattt tgattgtttt attaataagt tctattattt
     9181 atggagttta tatgcctatt caatacaaat taggatatga aacaacaaaa ttctttttcg
     9241 tgatgataat catggcttca tcatttgtat taccattaac tatagctcac agcaattttg
     9301 aaattccatt tatatatcag tatccatcca tgatagtgaa tattgtattg ttccttttaa
     9361 gtttaattat actgttagct tcaatgttta tttctattaa aatttttagt aataaggagc
     9421 ttgtataagg taagggggaa ttttatatgc cacatttgcc agattgggct gttttacttt
     9481 gtatcttact tccaatagta tataaagctt gtcagatatt taaaagtaaa aaatagagta
     9541 catatttata ttgccatttt tattgagtga agttagatgg tatgaattta aaaggttact
     9601 tgaaaataaa gttacttggt gtgggagagt aacatcataa tattatagat tatatgtttc
     9661 ttgtgaatca atttgtattt tttaatagat aatatccagt aattttattt tccattgatt
     9721 attttcaatt atgagtatga ttgtgtgtat tgtataccat cttttaaggt gtttttaaaa
     9781 aattatatcg tcaaaatata aatctaagga atcctataaa taaagctttt caattttaga
     9841 aaactaataa aaagtaggaa gttaataaaa agaagctgtc tcaaaataga gataagcttc
     9901 tttttcattg ctgaaatgac gaaaatataa ttgatttaat ataaaaaaga gtgctttatg
     9961 aactatttat tttgaacagt agatacactt gcactcaatt tagttactcc taagctttat
    10021 gaactaaaag tttattttga agcctatttt tctaaataat catctatata cagtattgcg
    10081 tttcttttat ttattcataa ttt
//
LOCUS       DAEPOG010000141         9736 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEPOG010000141 DAEPOG010000000
VERSION     DAEPOG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.141"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..211
                     /locus_tag="KR432_002837"
     CDS             <1..211
                     /locus_tag="KR432_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641356.1"
                     /translation="TGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKLDKADFM
                     LKKALSLDVENEDTIYNIEYLKSLR"
     gene            235..2112
                     /locus_tag="KR432_002838"
     CDS             235..2112
                     /locus_tag="KR432_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0641357.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            2105..4219
                     /locus_tag="KR432_002839"
     CDS             2105..4219
                     /locus_tag="KR432_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0641358.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            4228..5223
                     /locus_tag="KR432_002840"
     CDS             4228..5223
                     /locus_tag="KR432_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBH0641359.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            5315..6547
                     /locus_tag="KR432_002841"
     CDS             5315..6547
                     /locus_tag="KR432_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0641360.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            6567..7511
                     /locus_tag="KR432_002842"
     CDS             6567..7511
                     /locus_tag="KR432_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBH0641361.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            7605..8012
                     /locus_tag="KR432_002843"
     CDS             7605..8012
                     /locus_tag="KR432_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBH0641362.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            8099..9190
                     /locus_tag="KR432_002844"
     CDS             8099..9190
                     /locus_tag="KR432_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBH0641363.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      9499..9589
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         3956 a          856 c         1679 g         3245 t
ORIGIN      
        1 aactggcaaa ataaatgatg cgaaatcatt ggtagatgac tattcaaaaa catttatgga
       61 tgatgtaaaa attttaaata taaagggaat tttatatatg tttgaaaata agcttgataa
      121 agctgatttt atgttaaaaa aggcattatc attagatgta gaaaatgaag atactatata
      181 taatatagaa tacttaaaaa gtttaagata agtcaaaaat gggagaattg aagaatgaat
      241 acaccaatag tggttaatga aaaatctaaa gatataataa aagattggca atatacgtat
      301 acaaacgaag atataaaatt tacaagcata ataatattga catataatca aatagaatat
      361 actaaattat gtattgaaag tattaggaag tttaccccaa agaataaata tgaaataata
      421 ataattgaca ataactcttc tgatggaaca gttgagtggt taaagggaca atctgacata
      481 aaatctgtat ataataacaa aaacctagga tttccaaaag gatgcaatca aggtattgag
      541 ttggctactg gtgaaaacat attgttgtta aataatgatg taatagttac tccaaattgg
      601 ctttataatt tgaataaagc tttatggtct caagataata taggtgctgt aggtcctatt
      661 acaaatgcat gttcgtacta tcaacaaatt gatgttgatt ataaatcaat tgaggatatg
      721 tttatgtttg cacacaaaat aaatacatct aatattaacc tatggaaata tagattaaag
      781 ttagttggat tttgcatgtt aataaaaaaa tctgtgctgg ataatgtagg ggttttagat
      841 gaattattca cacctggaaa ttttgaagat gatgatttat cttttaggat gctagagtca
      901 ggatataagc tattattatg ccaggatact tttatacacc attttggaag tgtatctttt
      961 aaagaaaatc gtaataaata tacagatata atgtatatta atagaagaaa atttgaaact
     1021 aagtggggat ttaatccaga atatagtgga atgataagaa cagatttgat ttctaaaata
     1081 gatgatagtt tggaggcaaa tattaatgtt ttagaaatag gctgtggtat aggggctact
     1141 cttttagaaa taaaaaatat atataaaaat gcatcaatat atggaataga aattagtgag
     1201 tcagcaggta aacttgcaaa gtatagtgca gatgtactta tagcagatat agaaaaagta
     1261 aagttggatt atgaaaaaga tttttttgat tatataatat tgggagatgt acttgaacat
     1321 ttaactgacc catggaagat agtcaatgaa ctgaaacaat atctaaagaa ggatggttgt
     1381 ttgatatcta gtataccaaa tattatgcat gtttctgttt taagaaacct tgtgttaggt
     1441 aattttacat atgaagattc aggaatttta gacaagactc atttaaggtt ttttacatta
     1501 aatgagataa caaaaatgtt taatgatagt aattacataa taaatagtgt tacagctact
     1561 agtatgccac tttcggaaat tgataataga ttcattgata caatgtgtaa ggaaagctgt
     1621 aaagaaatga gacaacagta cgaatcttat caatatatag taaaagccac taaaagcata
     1681 gatactaaaa gatattctag tattagtatg gttaatttaa aatacgcttt aatgagaatt
     1741 gataatgaat tagatgtaga tagtaattta agttatatat ttgataatta taatataact
     1801 gaaccatgtt ttgttgatga tattgaatat ttagttgata cgtttgtgat aaataaagca
     1861 actctattaa atagattagg agttgaggca tttaacagag gtttaggaga ttttagtata
     1921 aacatgttta tgacagtatt agagatggat agagatgata ttgattcaat atataatata
     1981 acaagtgtat tatgtgaatt agaagaattt gatactgctt acaacttaat taaaaatagt
     2041 agcaaaaaag tacagagtga aagtgacata caagatatac tgtattttat tgaggggaag
     2101 agatatgaat aatgataaga tatgttttat aacctgtgta aatgatgaaa gactttatga
     2161 ggaatgtctt gtatatataa atagcctaga cattcctaaa aactttagca tagaaacagt
     2221 agctttaaga ggagccgatt ctattgcctc tgcttataat aaagctatga gagaaacaga
     2281 ttctaagtat aaggtatact tacatcaaga tgcttttata attaataaaa atttcatata
     2341 tgacatttta gagatattta aaaaaaatac agatgtagga atgattggtg tagctggagt
     2401 aaaagatata cctgtgaatg gaatttggtg ggattctgat aaaaaagcag ggaaagtata
     2461 tgatagccat acaggtaaaa tggatattta tgaatttagt aactttgaag aatgttttaa
     2521 tgaagttatg gctatagatg gattaattat gattactcaa tatgatgtat tttggagaga
     2581 ggataagttt gatgggtggc atttttatga tttgtcacag agtatggaat ttagacaaaa
     2641 taaatacaaa gttattgtac caaaacaaaa taagccttgg tgtatgcatg attctggagt
     2701 agcaaatata aataatggat atgatgaata taggataaaa tttgtaaaag agtacataaa
     2761 tcttgattta tatagagaaa atttttatga atttgataag aagaaagcaa gtaccatact
     2821 taagagtaat aagcatgtaa tggattgtat gaaagaggag cttacagaac aattaagatt
     2881 aaagaattat aaggatgttt tagatattgt ttctaaaatt gctcattttt ctgtttttaa
     2941 tcatactggc ttgtattctt gtgaaaaatg tgagagagca ttgctagaat gtgcaaataa
     3001 tttaccagaa atagaaaata cgaaattatt tacaaaaaaa gatttaaaaa gaagaaaagt
     3061 attacatgtt ttaagtgaag gatattcaac aggagggcat acaagactag ttaaaaattg
     3121 gattgagatt gacaaagatt ctgttcattc agttgtaact acatggcaat cagatacact
     3181 gccagactgg ttattaaaag caatagatga tagtggtggc gaagttatat ctttggattt
     3241 aatttcagat gattatttaa aaagagctgt agctttgagg aaaatagctt atgactgggc
     3301 tgatatagtt attttatatg ttcatatgtg ggatacaata cctgttgttg cttttggagt
     3361 tgatgggaaa atacctattt tacatgttaa tcactatgat agtacttttt ggttagggac
     3421 aagtattgca gatattgtca ttgattttag cgaagctagt caattaatta cattagataa
     3481 aagaggcata gataaatctg aaatattacc aatgccatta aaagaaaaag aagtattttc
     3541 aaaatctgaa gttagaaaac gatataattt ggaggatgat gtgacaataa tattgactat
     3601 tgctagtgaa agaaaataca atagtgtaaa tgatattaat tactttaaaa ttgctaaaac
     3661 attgattgaa aatacagata atacaattat tattgttgta ggagttgata cagagaatct
     3721 ctattggaaa actttaagta acgttacaga taatagagtt atatgttttg gaacaacagc
     3781 aaatatagac gatttttata agatttcgga tatataccta gagtctttca tggtagggag
     3841 tcatacttca aaattagatg caattttata tgatttaatt cctgttaagt ttaaaaatga
     3901 agtttcacca atttttacaa atatgtggaa ttttatagat aagtttgatt ataagaatat
     3961 aaaagaggtt atagacatga taagaaggta caatactagt gaatttgaca gggataagat
     4021 aaaagaaaaa caatcagaaa taaaacattt tgttatagat aaacactgta ttgaaataaa
     4081 aaactatata gacaaaatat ataaagaggc taatcatcat aaagttaagt atgatttata
     4141 taaaaatgat gaaatcgaag actatcagct ttttgttgca ttatatggtt atatggtaaa
     4201 aaataaaaac atgtcatagg tgataaaatg aacaatattc atatcaataa atttgaaaaa
     4261 agctgtgaaa aaaactggga ttattttgta gaaaataaat ctataaatgg tacatttctg
     4321 caaactagaa atttccttaa ttatcatgaa gaaggaaaat tcactgacca ctcattaata
     4381 atatataaag gtgaagaaat actagcagtc gtacctgcat gtgaaactta tgaaaatggt
     4441 gaaagagtat ttttttctca tttaggaagc acttttggag gtattataat aagaagtggt
     4501 tttaatgata ttaggcatgt aaatgctatt acaaataagt tagaaacata tttacaggaa
     4561 caaagtttca ataaagtata tttaaaaaat acatcaagaa tattttgtaa agaggatact
     4621 tcattactag aatattttct atttaagaat aattacatgt tttatgatga attaagtatg
     4681 tttttagaat taagtaagta taaaaaagat atattagcta attttagata tagtaaaaga
     4741 agatatttta aatattcttt gaagaataat cttaccttta aaagattgta tagtgatgag
     4801 gatatagttt gtttttttaa tttactttct aattgtttag atagtaaagg agctaaagta
     4861 atacattcag tagaagaact tttggagttt aaaaataata gattaaaaga tattgtagaa
     4921 ttttatgcag tatatattga tgacattatg atttgtggga gcatggtatt taactttcaa
     4981 gaaagagttt atcatactca atatctagca tcaaattcga catataataa atattatcca
     5041 atgatatttt tatactataa tctaataaaa gatgctttag agagaaattt taagtatctt
     5101 tcttttggaa ttagcactct aaatagaggc aaatacttag acgagggttt agctacattt
     5161 aaagaaggtt ttggttcaag tgcaatttgc aacagaacat actataaaaa tataaataag
     5221 tgataatcat gttttataga gtttttagag tatagtaaat atctagtact ctaatattac
     5281 ataaaaataa ttagtttata aagaaggttt gtatatgaaa agattaatga ttttaggctc
     5341 aattaagtat tttgagaata ttgttatgtc agctaaaaaa gagggaatat acacaattgt
     5401 ttgtgataat agaattgata ctcctgcgaa aaagatttgt gatgaagcaa tagatgttga
     5461 tgtatttgat tttgataaaa taaaaagtat agctatagat aaaaagatag atggaatttt
     5521 aactggattt acagattctt taatgaaacc atatgtttat attgcaaatg aactaaattt
     5581 accatgtgtg atttcttgca atcaacttga atctgtaact aataaggcta ttatgaaaaa
     5641 atattttaaa aataatgcaa tacctacaac tgattattgt attattgaaa gtctaaaaga
     5701 tttagaaaaa atacaaggat taaattttcc acttgtaata aaacctgttg atggttatgg
     5761 ttcaagaggt gtatattttg tatcaaatat agaagaatta atagaaaaat ttgaatattc
     5821 taaggaagcc tcacaagatg gacaagtaat aattgaggaa ttttatgaaa gtgaggaaat
     5881 acaaggacta gcttgggttc ataatggaga gtcacatgta ttttatattg gagatagaga
     5941 gcttgtaaat atacatacag gaagagctgg aaaaccagat agattacttt atccatcaaa
     6001 gtattgtttt caatatgagg aagaaataaa atctatatat caaaatataa cacaggcttt
     6061 tgatataaaa aatggacctc tttatgtaca aatgttagtg ggagcatatg gaataaaggt
     6121 atctgaagta atgccaagat tacctggagg ttgtgactat atggctattt ctcaaataac
     6181 agatttagac ataggaagtt tatttgcaaa ctttagtgtg aataatgaag taaattttga
     6241 tgaaataaag aaatataata tgcatttatc aaaatgtgta tatgcattac caatatacat
     6301 aagacctgga acaataaaaa agataaataa tatagataaa atagaaaact tagattatgt
     6361 cactcaattt ctattattag taaaagaagg agatacaata ggattatatg aggatgagag
     6421 acaggattgt ggaagagttt atggaatagc aaataatata tttgatgcaa atataaagaa
     6481 gaaatgtatt catgaaatga tagaaatatt tgatgaaaat aatgaaaata tgatagaaaa
     6541 tttttaaata agggagtatg atagatatga agctaataac tcatgaacaa attatgtcat
     6601 taaacatttc tcagaaacaa ggatatgaat ggataaaaga atctttttta acaaaaaaag
     6661 attgtcaatt accaccaaaa ataagcatgg tagaagaagg tcatgtattt tacaatgtaa
     6721 tgccgtcctt aaatcataac catatgattg gtggaataaa aatggttaca agattgccag
     6781 ggagaaatcc aagtttaaat tcagatatat ttctatacaa tatgaaagat gctaaattaa
     6841 aggctatttt ggatggtaat tatattacag cttttagaac tgctgctgtt gctggtttgg
     6901 ctattgatac ttttgctaaa aaagatttta atactatttc tattatgggt ttaggtgtaa
     6961 ctgcttctgc tataatgaat atgctttcgt atactcttaa agataaaaat ataaatataa
     7021 agttacttaa atataaagac caagcagagt catttataaa aaagtatgaa aaaaatgaac
     7081 aatttagttt tgaaatatgt gatacacatg aagaacttat tgtagattca gatgtagtta
     7141 tttctactgt gacatataca gatgaatatt tatcagaatt taaatggttt aaaaaaggtg
     7201 ctttattatt acctgtacat gttatgggat ttggaaatgt tgattatcta tttgataaag
     7261 tatatttaga tgattataat catttgaaac atctagaaaa tatagataga tataaatatt
     7321 cttgtgaatt ttataatgtt ttaagtaaac aagctaaagg aagagaaaat gatgatgaga
     7381 gaataatatc ttacaatata ggtttgtcaa tacatgatat attatttgca aacaaaattt
     7441 acgaattgtg tgaaacacta aattgtgaag agatagaatt aaaagaacca aaagaaaaat
     7501 attggatata gtttatttca ttcatattta caataattta ttgtaaatat gaatatgaag
     7561 taatatattg ttagaaaatt agcatgaata ggatagataa atttatggat aaatataatt
     7621 taattaagtt cttatctatt ggaaacgaat atataggaaa tttagttgca ttagaatcaa
     7681 ataaagcaat accttttgat ataaagagag tttattacat atatgatgta ccaaacaatg
     7741 taaagagagg atttcatgca cacaaaaact tggaacaagt actagtttgc ctaaatggaa
     7801 gtgtgaagat aaaatgttat gatggtgtaa aagagagtat atttaattta cataaaaatg
     7861 atgttggatt gtatatagga acgggagtgt ggcatgagat gtttgacttt gatgaaaaaa
     7921 ctgttttaat ttctatagca tctgaacatt acaatgaaga agattatatt agagactata
     7981 aagaattttt aaagtattta aataatatgt agttattata cagttagtga tgtattttct
     8041 taattaaata aatttaagat agccatatag agaataaata gattaggatg tgaatttatt
     8101 gttggttaaa tttaataatt tattaaaaca gttccaaact cataaagaag aatatgaaca
     8161 gaagattaag gatgtaatgg aaagtggata ttatatcctt ggaaatgaag taaggcattt
     8221 tgaagaagaa tttgctagct atgtaggtag caaatattgt gttggtttag ccagtggttt
     8281 agatgcactt ataattgctt ttagagcatt aggaataagt gaaggtgatg aagtaatcgt
     8341 gcaatcaaat acatatattg ctagtgttat gggaatcagc ataaatggag caacaccagt
     8401 atttgtagaa ccaaatgaat attataatat agattcatct aagatagaag aaaagataaa
     8461 tagcaaaaca aaagctatat tagtagttca tttatatggt caagcaagta aaatgaaaga
     8521 tatacaagaa ttatgtgata agtataactt gtatttaatt gaagattgtg ctcaatctca
     8581 tggagctaaa tatgatggaa aaatgacagg aacatttggt gatataggtt gttttagttt
     8641 ttatccaagt aaaaatttgg gtgcatttgg agatgcagga gctatagtaa ctagtaatga
     8701 ggaaatagct gataaagtga aaattcttag aaactatggt tcagaaaagc gatattataa
     8761 taaatttgtc ggatataatt caagattgga cgaaattcag gcaggtttat taagagtaaa
     8821 acttaaatat ctaaatgaaa ttgaaattga aaaatcaaaa atcgtagata gatatcttaa
     8881 tgaaattaat aatcccaaag tcatacttcc aaagatagaa aataattgta ctcatgtatg
     8941 gcacttattt gttttacaag ttgaaaaacg agatacattt atggaatact taaagaaaaa
     9001 tggtatagag tctataatac attatccgat accaccacat ctatctgaag catatgctta
     9061 tttgggatat aaaaagggga gttttaatat agctgagaac tattcagata aagttgttag
     9121 tataccttta tttgtcggta tggaagatga agaagttagt tatataatta aaattataaa
     9181 tgaatattaa actaatcata ataatatcaa aaataaattt tctatatatc atttatttaa
     9241 atttcgttaa ttgacatttt taaatattga tatattgtac aaataaaatt gaaatatatg
     9301 gaagaatata gaataaagat gagatttgtc taatattttt ataaattaaa aatattttca
     9361 aagagtttat tataaaattg tagactatag tgtttataat ctttgtaatt attggtaact
     9421 aaatttaaaa aataaaatat ttaattatac acaaaaaaga tatttaaaaa atttgttatt
     9481 tattgtatac ttaagttaaa actaaatagg caaatctaga gaaatctagt gacgcaaagc
     9541 tataggggct aaggtttgta tacacaaact atgtcagcca gttgccaaaa agagttctag
     9601 gtatattgta tacgtaggaa gagtctagga tgactgcctt tttggtggtc attttgttat
     9661 ggaaaaaatc acattatgta gtaaaaaaca cctataataa catattatac caaatataat
     9721 ttaaaataaa gataaa
//
LOCUS       DAEPOG010000142         9584 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEPOG010000142 DAEPOG010000000
VERSION     DAEPOG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.142"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            237..612
                     /locus_tag="KR432_002845"
                     /pseudo
     CDS             237..612
                     /locus_tag="KR432_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            1224..1916
                     /locus_tag="KR432_002846"
     CDS             1224..1916
                     /locus_tag="KR432_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641364.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            2112..4172
                     /locus_tag="KR432_002847"
     CDS             2112..4172
                     /locus_tag="KR432_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0641365.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(4378..4701)
                     /locus_tag="KR432_002848"
     CDS             complement(4378..4701)
                     /locus_tag="KR432_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBH0641366.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(4694..5395)
                     /locus_tag="KR432_002849"
     CDS             complement(4694..5395)
                     /locus_tag="KR432_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBH0641367.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(5673..7058)
                     /locus_tag="KR432_002850"
     CDS             complement(5673..7058)
                     /locus_tag="KR432_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0641368.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(7166..8404)
                     /locus_tag="KR432_002851"
     CDS             complement(7166..8404)
                     /locus_tag="KR432_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0641369.1"
                     /translation="MELFIEVIKFNIYISLLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(8417..8785)
                     /locus_tag="KR432_002852"
     CDS             complement(8417..8785)
                     /locus_tag="KR432_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641370.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(9313..>9584)
                     /locus_tag="KR432_002853"
     CDS             complement(9313..>9584)
                     /locus_tag="KR432_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBH0641371.1"
                     /translation="VEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIES
                     ESKRNIFGKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
BASE COUNT         3703 a         1075 c         1290 g         3516 t
ORIGIN      
        1 acttttaaga aaaaggtact aaaatctagt aaaagcataa aaataatgcg ttcacttacc
       61 actacacata aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaat
      121 ttaaaagaat gatatagtaa aaatcgaatg taattattta ccttaattct tttgtttgta
      181 aaactttatt gatatagaat ttaacatata cataaattgg taaagggatg tatactatga
      241 aaattagtaa attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa
      301 aggtattaat atcaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata
      361 caactatatt tacagtatta aaaagattac taaagaagag agtttttaga aacggagaaa
      421 atagataagc gtacacacta taatgtatta gtaaaagaaa aataatatca gagattttaa
      481 acaaaagaat ttttaaagaa tacacataaa aattcaatat taagtttaat gtcagcacta
      541 tgtagtaagg atgatgaagt agatggtgat attttaaatt tattagaaga atattttaag
      601 aatttaaaat aagaaaatga cagctagata cataataaat aaaatgaagg tatgaaatta
      661 taactttttc tacattcatt ttgtttatta ttctgaacat gttaaaaaaa tgaaataagt
      721 ttgtatttta tttatataaa ttttaataga atatgtacag tatatagtag atagaatgta
      781 taaaaataga atttaaataa gtagctatta ttgcagtgaa ataaatgttt ctaagtatat
      841 ttgggtggaa gaatggtgga agaaaattag tgaaatgtaa ttcttccacc attcttccac
      901 cccaaaaata aaaactgttc aataagaatt aatatacatt aattcagctg aatccaatca
      961 aaatacttaa ataaaagtaa tctgaaaata acataattca aatcaattta ttcaaataaa
     1021 tctcaattta tcttaaatct taagaaatat ttaagaatta ggataatatt ttcttaagat
     1081 ttatatttta ttatatagtt ataagggaaa gttacaaaaa tgtaagccaa agtaatataa
     1141 caatttagtc attgttgaga aagtaagtga aagatattgt ataatataga taagtattat
     1201 tatacaatag aggaagtgaa aatatgtaca atatattagt agtagacgat gataaagaaa
     1261 ttgttgattc aatagaaata tatcttaaat cagaaggatt taaaatatat aaagcatatg
     1321 atggaatgaa agctttggaa ataataacag agaatgacat tcatctaata ctaatggata
     1381 taatgatgcc taaattagat ggaatcaaag caactgtaaa aatacgagaa gaaagaaata
     1441 ttccaataat tttaatttca gcaaaaagtg aagatacaga taaaataata ggattaaata
     1501 ttggagctga tgattatata acaaagccat ttaatctatt agagcttata gctagggtta
     1561 aatcaaactt aagacgatat gtaactttag gtaattatga agatacatgt aaagaaattt
     1621 taaaaagtgg agctttggag ttaaatacac taacaaaaga agttaaaatt gatggtgaaa
     1681 acataaaaat gaccccaatt gagtataaaa taattgaact tcttttagaa aataaaggac
     1741 gagttttctc aatagatgag atatacgaaa aagtatggaa tgaggaaagt tttaattcag
     1801 aaaatacagt agcagtacat atcagacgaa taagagaaaa gattgaaata aatccaaaag
     1861 aaccaagatt tttaaaggtg gtgtggggag ttggttataa gattgaaaaa ttttagtgga
     1921 aaaattataa aagaaaagag tttaatattt aattgcattt tgattttagt catttttaca
     1981 tttacacaaa tgttaggaat tttaatatgt tttaattttt tctctgagag tttaatttac
     2041 tctatgggga ttatgctttt cttaaacttt attttatata aaatactgac aatgtagatg
     2101 aggtgggcaa cttggaaaaa tatagagcat taaaattagt ttcattaatc ataatatcta
     2161 tctcatttcc attaactata ttgttggcta caataggact tgctgagcct aaatacaata
     2221 tacaaaaaac ttctacaaag agtgggattt ttaatatatc taatgatttt tatgatagta
     2281 atatatttga tgaaggaaca gtggaaagct tattatctaa ttttaagata tctgtggata
     2341 gtcagttaac tagtgaagaa aaaagtaaac tccaaacaaa aagtaaaagt atagatttaa
     2401 gtagtaatga ttctgagtat gataaaatca tgattaataa acaagcttct gaagaatatt
     2461 taagttatct aaaaaatata aaagtgtatg ttattaatac atatacagga gtattttata
     2521 caaatacatc atatggtaat ttagatgatt ttaaacaaaa aactaagagc tattgtaata
     2581 tagagataat aaataatgta ggaaagagct cgtatattaa aacaataaat ggagaaaaat
     2641 ttgaactgaa taatctgagt atggaactaa atggagtaga tgaagccttt gaggcatatg
     2701 tctcttttcc agaagagcca actatagaag atggaatagt atacactaat tttcaaatat
     2761 ttaaacaagc tacagaaaaa gtaagattat tttttgtagc ttgtttaagt ttagcaattg
     2821 tgtgtttatt atctatcatt ttatatagca gaataaaagg tgaagtttta aaaagaaata
     2881 atgtattttt actaatatac agtaagatac ctatggaatt taatgtaata ctagtattta
     2941 tttcaattgc aattttaact atgtatgctc ttgctgtatc ttatcatatt gatgtaattg
     3001 tattagtttc tttatcttta ctgatagtat caaataatat attttttata aatgtgcaat
     3061 tgagttattt agaaaataaa atgtgtatat ttaagacaag tattatagta agagtgattt
     3121 tatggttaat aaaaactgta agggaggtca gtaacgctat aaagaaagta tctttagcaa
     3181 ggaaagttat tgttatagtg ataatatctt tagctttaat catggtaatt agcactcgaa
     3241 gtataggtat gggattactg ttttctatat gtattttttt aggatttaca atatatataa
     3301 ctaaaagact ttcatatctt agttacgtaa tagaaggtac agaaagaata aaaaagggag
     3361 aattagatta taagataaaa attactggga atgataattt tacgtcttta gctgaaaata
     3421 taaataatat tggagaaggt cttgatagag ctatagaaga gcaagtaaaa agtgaaagaa
     3481 tgaagtctga tcttataaca aatgtatctc atgatttgaa aactccactt acttctataa
     3541 taaattatgt agatttaatt aaaaaagaag aatcaataca accagagtat ataaatgatt
     3601 atataaaggt acttgagtca aagtcaaaaa gactaaaatt attgatagaa gatttatttg
     3661 aagcgagtag agtaagtagt ggcaatatag aattacaaat tagcaagatt gatttagtgc
     3721 aactattaag acaaagcata ggtgaacttg aagaaaaatt atctaaaaat aatttatatt
     3781 taaaattaaa tgttccaaat gataaggtgt atatatgggc agatggaaga agaatgtata
     3841 gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa actagagtct
     3901 atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat atatcttctt
     3961 atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct gatgaatcaa
     4021 ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta gctttacaag
     4081 gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta gagtttcaaa
     4141 aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat atattttttt
     4201 tactgtcgaa agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa
     4261 aaacagagta actttaagtc actctgtttt ttatttaaaa tcaataatat aacaacttaa
     4321 attaaaattg tacacatagt tttaaaataa cttatacaat aaaacattat ccaaatatca
     4381 caagataatt ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga
     4441 tttaaactta tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat
     4501 agataaatat agactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat
     4561 tgctacaggt ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa
     4621 aattaacata ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa
     4681 aaatatataa ttattattca taagattcct cctttttaca gtaagattct tctataaaga
     4741 aaccaattaa agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg
     4801 catataaaat aacagaaata atgattgatg atataaatac tacaatataa atcttatttt
     4861 ttatttgaga aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac
     4921 tgtctataga tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa
     4981 ttaagcagaa gaaattaaga taaatagcat ttctatcaga ccaatttcca gcagtaaatt
     5041 tttcataatt aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt
     5101 ttggactttt attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt
     5161 taactataaa tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag
     5221 ctgcaatgaa ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag
     5281 gtgaaagacc agatttttgt aatagtattc ctcctactat acctataggt atataaccta
     5341 agaatatagg aattgaagca ataaagcttt cttttaattg ttgagttctt tccacaataa
     5401 atcactcctt ttgtattaaa tgtattaaag tattagtaaa acagaaaaat agattaattg
     5461 tataattgca attataatta aaaataaatt tggattaata tataaaatac tatacaacaa
     5521 ataaataatc ttaaacggtt attaaatttt atctatttgt tattatgatg taataataca
     5581 tattaatctc tataagttaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa
     5641 tacacacaat taaaatattg tgtgcaatac aattattttt tctcttctaa tagtcttttt
     5701 aatacgctca gaccagcttt aagttcttct gtgctttctg tagcacatac agctattctt
     5761 attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacctgtaca
     5821 ccttctttaa gtgcttgtat ctcaaatttt tctgccatta ttccatcagg tagaatcaac
     5881 catctaaata tacattcttc atttcccaaa agattatatc cagataaata ttgattaata
     5941 atcttgtttc ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct
     6001 gatgtaatca tgcgataagc aatttctgtt aaaaaataag attgagatag attaatgttg
     6061 tataatgcgc ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt
     6121 aaactaggtg caataatttt tgataaactc gtaatataaa ttgtttgatt gggcagatag
     6181 gaagccactg gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca
     6241 attaaattgt atttagatgc aatatttgca atcatttttc gtccattttg tgacattata
     6301 tgtgtagttg gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta
     6361 caagcatata atagtccttc ttcactaatc tcattatctt cttgtttaat tggaattagt
     6421 tgaatcccaa gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct
     6481 attctatctc catgttgaaa caaaccagct aaaatagcaa caattgcatt ttgtccacca
     6541 cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag ctttataaat taatttggca
     6601 atagcttctt tttgccattt agcaccatct ctttgtccat attggaatat agttttaaaa
     6661 tctatttcct taattatatg tttaaacaaa gtattcattt cttctagtgt gaaatctgga
     6721 ttcattgtgc ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt
     6781 gaacgcgtat cataagatac aaaagttcca cttcctgtaa ctccacttat tagacctttt
     6841 ttttcacaaa ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc
     6901 aattcacgtt ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt
     6961 tctagttgtt ctgcaagcgc cttatataat atttgccctt ttgaaggtct ctttggtttc
     7021 cagttcatag gataattttc aaaagaatta attggcataa gttttcacct cacattatta
     7081 atttgatttt ttctttatct aaaacaataa attctaaaga tacaaataaa aactgatagg
     7141 aaataattct atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac
     7201 cttctttatc ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat
     7261 ataaaacagc ttcttctggt gtctttggtt gattatcaca atcgtacaaa tctctttttt
     7321 ttacgtagcc gactttttta cctctaccta ttgtttgtat aagttctggt tcaatggaat
     7381 ttccatttga gtctattata tgtgtaccat aggtttctcc atttttatta taattaatct
     7441 tagcaatagt atattctaca tcttcatttt gtttaaaatt ggagttgata gaaaatattg
     7501 accctattat tataaaggtt aataaaaact taagacctaa attaattttc tttttatctt
     7561 gattaaacat agaatttaac ctctttttta aaataatatt ttttttattt agaaactgag
     7621 atacgaatga tgaatacttc aaagtttttt gatactttat tgtatttaat attatatatg
     7681 catactcttt aatttcactc gtggtacatt tgtcaatcac aatttcatca caagataatt
     7741 cacataattc atgaaaatat ttttttaata agtataaaat tggattgaac caatgtatga
     7801 tacatattat actaattaga agcttaaata agttatcttt tcttttataa tgaattaatt
     7861 catgtttaaa gataaatctt aactctttat tattgtactt tttttctgga attattattt
     7921 tacagcgtgt taaacctata atggcagggg aataaatacc tttaacctta tatatctgaa
     7981 gttttttatt tatacttagt tcttttgctt gttcatctaa gatactagta atatctattt
     8041 ctaaaatttc ttttttaaaa ttttctatgt aattataaaa tttaaaattt gttcttaaat
     8101 agtaaaaaga gattgctata attcctaata tccagatgta cataaatata tctaaagtaa
     8161 tgccctggtc atatggtata taattatcga gattatgttt atagatatta atagtagtat
     8221 tattgatatt taatatattt aaatttattt tagatataaa catcattctg actactatta
     8281 aaagacatat aaaataatta aacttaaaag tatatttgct tatcttttta gtaaataaaa
     8341 tgaaaaaaag aattagtaga cttatgtaaa tattaaattt aataacttct ataaataatt
     8401 ccataaaata cgtctcctat tttctttttt ttagccattg ttcaagttct tttatgtcat
     8461 catctgatat ttcatttcca caacctaatg atgatataaa gctatgaata gaatttttat
     8521 gtataagctc taaaaaatcc tttgtttcaa attttagata ttcctcatgt ttaactttaa
     8581 tagtgtataa agtatttcta ccatcttttt caaattctaa aaaaccttta tcacttaatc
     8641 tagaaagtac tttaccagta gtaccttttg tccatccatg agaaatttcc atataattag
     8701 ctacttctct ggctggtatt ttatctatgc ctttatccca tatgaaattc ataactaaca
     8761 actcggattt tggtattttt ttcaatataa tcactcacct ttaacttgat tatattagac
     8821 aaatgtctaa tagtcaatat aatatatcat catgtataaa gaatctttat attattttat
     8881 gcttgagaac taagtagtat agatttatag ataaaaaaga tattgagagt tttatggaag
     8941 aggggaatat attttctatt agagataaga atatgacata tattttttat tgatttgtac
     9001 aaaaatacta tatagtgaat aaattaatat cttttctaaa ttagaagagg ataatcttat
     9061 tagataaaga ttatcctctt tattgaaagt ttaagggata ttagagatta ttaatttttc
     9121 tctctttttt taactaaaag caataataca taaaattatt attgctaatg atactcttaa
     9181 tgtggtatat agtcatagtg tcacatgttt atcaatagtt ttttcattaa ttattgtaac
     9241 gaatagtagg actcataaaa tacttagaaa tatgttttta gatagatttt aaatatataa
     9301 tctaacttat atctataaat taacactatt attctccaat tccatttcta gtttttttat
     9361 aaatttatta aattcttctg gttgcctttt tccaaacaat actcttacaa gagctgaatt
     9421 gatttttcca aatatattcc tcttgctctc actttcaata aaaataagtt ttgtattttc
     9481 taagtcaata tgtactaatt catatctagt aatagtactt tctcttttat tgcttgtttt
     9541 tatctcatat actctatttt tttcgaaatt agttatttca acct
//
LOCUS       DAEPOG010000143         9561 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEPOG010000143 DAEPOG010000000
VERSION     DAEPOG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.143"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..363
                     /locus_tag="KR432_002854"
     CDS             <1..363
                     /locus_tag="KR432_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641372.1"
                     /translation="FKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITL
                     NERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNI
                     SVESIPGLGSSFYITFPI"
     gene            583..3060
                     /locus_tag="KR432_002855"
     CDS             583..3060
                     /locus_tag="KR432_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0641373.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3075..3761
                     /locus_tag="KR432_002856"
     CDS             3075..3761
                     /locus_tag="KR432_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641374.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4034..4867
                     /locus_tag="KR432_002857"
     CDS             4034..4867
                     /locus_tag="KR432_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641375.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            4905..5366
                     /locus_tag="KR432_002858"
     CDS             4905..5366
                     /locus_tag="KR432_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0641376.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            5519..5944
                     /locus_tag="KR432_002859"
     CDS             5519..5944
                     /locus_tag="KR432_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0641377.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            6397..6717
                     /locus_tag="KR432_002860"
     CDS             6397..6717
                     /locus_tag="KR432_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBH0641378.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            6855..7499
                     /gene="trmB"
                     /locus_tag="KR432_002861"
     CDS             6855..7499
                     /gene="trmB"
                     /locus_tag="KR432_002861"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH0641379.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            7628..8374
                     /locus_tag="KR432_002862"
     CDS             7628..8374
                     /locus_tag="KR432_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641380.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(8503..8781)
                     /locus_tag="KR432_002863"
     CDS             complement(8503..8781)
                     /locus_tag="KR432_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641381.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            9056..9502
                     /locus_tag="KR432_002864"
     CDS             9056..9502
                     /locus_tag="KR432_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641382.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
BASE COUNT         3681 a         1029 c         1512 g         3339 t
ORIGIN      
        1 tttaagagta atatagataa tattaacttt gattttgatt gtacactatt aaaacgagct
       61 tttagtaatc ttattgtcaa tgcttttaca tatggagata aaaatactaa agtttgtgta
      121 catataacat taaatgaaag aattgaaatc attatatctg ataatggaaa agggatgtca
      181 agagaagaag catcaaattt gtttaatcgt tattatagag gaagcaatac aaaacaaaaa
      241 atagaaggaa ctggtttagg actagcaata acgaagcaaa ttattgaagt tcatggaggg
      301 aatatatctg ttgaaagtat acctggatta ggtagctcat tttatatcac ctttcctatt
      361 taattaaggt taattaaggt caaataaaaa atatattaag gttgatagat tattatattt
      421 atatagtagt ttatcaatct tttttattta aggaggtgat ttgattaaaa atattataaa
      481 tagaatgata aaagtatgga ggagtctatt agagattcaa tttggattaa agtataaaaa
      541 tattatactg aaatatctta gataaaggaa gaaggtgtaa atttgaaaat tatattaaag
      601 tatattttca caaatattaa agaacgaaaa attagaacag cagttatgtt actgtccata
      661 gttttatcaa cagttttact ttttgtttca ttctcaattg gattatctta tgaaagtgca
      721 caaagaaaaa tggcaaaagg tatgtatggt acagcgacta tatctgtcca aagtaaaaat
      781 ccagatgtat taaccaattt agaagacata cctgatttaa atgctataaa atcaaaggtt
      841 ggtgtattgg aaagctctgc tatttataat aaagggggat actatgaaga atttagtctt
      901 atatcagcag acttaagtca actaaataaa atcaacaagc cacgattaga aaatggcgat
      961 agtattactg atttttctgg tgataaaatt attcttccaa atcgctttac atcaaaatat
     1021 aaaataaaaa aaggtgattc tattacttta cagatttatg gaaagtctta tacttttcaa
     1081 gttagtgata ttgcatctta tgatacagta tttttgagga atacaagagg tgtaaatgca
     1141 ctgctaccca aagagactct atcaaaaatt ataaataaag gcagtggata tacaagagtt
     1201 cttatagagt cagaagaaga aatgacagaa aatctagtaa ataaattatc cgaagaactt
     1261 tccactgaaa aatatatggt atcaaatact ataaatgaaa ctaagataat ttcagatgct
     1321 agacaaaaga caatgccttt ctttttaata agtttttttg cattaaccct tagtatattt
     1381 atcatataca gcagctataa ggttattaca ttagaacgtc ttccttttat tggaacattt
     1441 agaagtattg gtgccaatga aaaaactgta actcgtatat tgatgcttga aagtatatta
     1501 tacggaagta taggaggatt aattgctatt cctattggtg tagttgtttt aaatttaatg
     1561 cttcatgggt taggaaattc tctcgaacaa ggaatatcaa ttccagtagt aatatcaccc
     1621 attggagtga ttatatctgt tatagtagca ataatcgttt cttcatttag tgcatatata
     1681 cctgttaaaa aagctagcca tttgccaata aaaaatattg tactaggaac agtagaagaa
     1741 aagaatgttt caaatcgttc tatacttttt ataggaagta taatgtttat tttatctatt
     1801 ttacttccaa gaatatcccc tgaaaataca ttgtatctag ctggtggatt ttcattacta
     1861 ggattaattg tagctactat tgtgttgatt cctcttatta ctgatatcat gtctatagtt
     1921 tttgagtttg tttataaaaa tattttaggg aatgagggta aattagctgc tagaaatatg
     1981 aaaaataaca aaaatataat acagaatatc actttactat ttataagtat ttctgcagtt
     2041 attgcaataa gtgttgttgg taactttgtc aaaacttata taacagatgt gtttcgtgac
     2101 gcagaattac aaggttttgc agatggaaag atgaatgaag agtttattga aaatgttaga
     2161 catatggatg gaattaaaaa aatattacct ctttatgtta tgaataatga gatttctggt
     2221 aatggtgtaa cattatcaag actagagggt acagataata taaaactata taactctatg
     2281 tttggaatca attatacaaa ttttgagata aaaaaacaag taatagaagc ttttaatgat
     2341 aaacgttctg ttattttgaa tgaagatact ctaaaaaaag taggcttatc tataggagat
     2401 acaataactc tatctaatga taagtatgat ttttcttata aaatagtagg tagttttaaa
     2461 tcccgtgcaa atgatgtaga agctgttata ccatcacatt atgcagttaa tgattttgat
     2521 aaaactaatt atggtttttt agtatataca gcagtcaatc cagatgttgt tatgattcag
     2581 ataagagatt tatttggaga tacatataat tggagtcgta cagttgaaga atttaataat
     2641 gattctttaa atactataag tagttttttg tctcctatga ataagatgac ctatttcatt
     2701 tttcttcttg ctacagtagg tattattaat aatctactga ttaattatat tcaaaaaaaa
     2761 cgttctatag ctatgtataa atctatagga ctaagtgata aacaaaatat aaaagtgaca
     2821 ttgattgaag ggtttacttc aggtttactt ggagctgtta ttggtattgt gatatctata
     2881 cttgagattc aaactatctt tatagttgca ggaccaaaaa tttcaatgaa accagattta
     2941 gattttaaaa catttattat tgttgggtta ttgggaatta tagtaacttt aataggttcg
     3001 atagtgccta ttataaaagg gaagaaaatg aaactgatag aagaaattaa gtttgaataa
     3061 ttatggaggt taatatgaaa attacacata atagtttagc tgtagaagca aaaaatatta
     3121 tcaaagaata taaaattgga aatacaacaa ctcgagtgtt aaaagaggta tctttacaag
     3181 taatgaaagg tgaatttgtt tctatcatgg gtcaatctgg ttctggtaaa agtactcttt
     3241 tatatatttt aggtggttta gatactccaa cgagtggaaa agtgtatatg aatggagctg
     3301 atatatcaca ttttaatgat gagaaaatga gcataatacg tcgtagaaat ataggattcg
     3361 tatttcaatt ttataactta ataccaaatt taaatgtgga ggaaaatata atgcttcctt
     3421 tgttattaga tggtaaaaat ttaaaagatt ataaaaatca attagatgaa atcttagata
     3481 tagttggttt aactgataga cgtaaacata caccacgaga gttatctggt ggtcagcaac
     3541 aacgtgttgc tatagctcga gctttagttg gaaaaccaga gattttattt gctgatgagc
     3601 ctactggaaa tttagatagt aaaacaggga tagaaataat agatttactg aataaaataa
     3661 acagagacaa tggacaaact ataattatgg taactcactc accagaagca gccaaaagta
     3721 gtagtcgtac tattacagtt tcagatggtt taattgtata aggactaatt attgtatttg
     3781 agataagcat ttttaactat ataaattaga catatgtaaa gtggatataa gttaaatact
     3841 ataaatgtgc tataatatta aaataacaga taaaagtact atatttttta ttgattattt
     3901 taatttattt gtttattaaa tatctaatga gtattcatac tatctctatt attaaaagca
     3961 gttaatgata tatagagata atatggattc ttcttacttt aataaatatt cacgatagga
     4021 caggatataa tatatgttaa aaaaagaaag tattcatttt ataaataaaa ataggtcact
     4081 tatagtttgt gaagacaatt ttgtttatct cttgccttgt atgcaattaa aaaattggat
     4141 atcgaatttt actatatcgt ttccaaatca aacaataatt tctgataatt acacgattat
     4201 tcctcatgga agtgtaactt tggtgttttt ttatgatgcc acagggttac atagtctatt
     4261 atttggtcca actactaaac caaaaacagt aggaaatatt gctaatagat gtgatgttat
     4321 actaattata gaatttcaac ctgctggttt tttcccgctt ataggtatac agcaaagtga
     4381 acttattgat aaagttgttc cattttctat aattaatact tcattagatt tagaaattaa
     4441 aaaaatattt aatgaatctt tatctataga taaattgatt ttaaagttgg aagaattatt
     4501 gatttctaat attaaaattg aatactcata tgagtttatt ttggcaattc agttgattat
     4561 tcaaaactct ggaaacatat cttcacaaga aatttctaaa aaagtatttt atagttctag
     4621 gcatttaaac agactcttta atcagtacct agggttgagt atgaagtcat tttctcgttt
     4681 ggtacgtatt aataaatcta taaaacttct aaacaataat aaaacttcat tgatgtatat
     4741 atgtaatgaa ttaggattct atgatatacc acattttatt aaagatttta aaattgtctg
     4801 tggaataact cctcaaaaat accgagctaa tatgtctgat ttttacagtg aaattgcaaa
     4861 attttaagtt ataatactta taacaaagaa aggatagtga taagatgaaa tttggaggag
     4921 tattgctaat tgtcaaagac ataaataaat caaaaaagtt ttatgaagat gttatggagc
     4981 aaaaaattac attagattta ggagaacatg tatcatttga aaatggattt ggattacaaa
     5041 gtaattataa agctataatt ggatatgatt taccaatata tcaacaatca aataactttc
     5101 agttgtattt tgaggttaat gatatcaaac aatgggaaag taagataaat agaataggaa
     5161 atattgaatt tttgcattat ataaaagaat acccatgggg tcaaagaact tttaggtttt
     5221 atgattttga taaaaatatt atagaaattt cagaaagtat ggaaagcgtc ataaagaggc
     5281 ttttaaaaca aggtttagct ttagaagaaa tttcaaaacg cactatgtat ccagttgaat
     5341 ttattacaca atttcaacaa ttataaaata ttaaaatata taattttttc tttatattgc
     5401 aaaaaacaaa ggcttggggc ataatagaaa aatacttgaa ataaattaca tacttaatgt
     5461 ttgaatttag cttaaaaatg aaaagacgaa gaaatttata cttggaggta attacgttat
     5521 gcatgatgaa ctgaaaatca aaatgtactc atttacgatg gattgcaaag acccacatga
     5581 attagcaaaa ttttatgcag cgttactcaa ttgggaaata gcattttata atgaagaata
     5641 tgcatgtgtt ggtgctccag gaacaaatca gggaacatat cctggtataa catttcaacg
     5701 aaattctgag tataaaccgc ctgtgtggcc agaaaaacct gaatctcaac agcaaatggc
     5761 tcatatggat ttcgctgtta atgatttaga agaagcagtt caatatgaaa ttcattgtgg
     5821 agctacaatc gcagaggagc aatttactga tgactggaga gttatgattg acccagctgg
     5881 acaccctttt tgcttatgtc agatgaaatc aattatggaa agtagccatt tttcattgtt
     5941 atagaccaaa atattgttta ttaattacaa aaaagcacaa cagtatatac cgttgtgctt
     6001 ttacttatta tacatgaata cactaaaaaa tataaatagt agagctttat caataatgat
     6061 aatttttaaa aaatagtatt tttttattga ataatttctt agatatttta aagtatataa
     6121 attttgcata tatgaattgg acattatcaa atgtgttata atattaaaac aacaaataaa
     6181 attattatat tttttagaat aataatttta tttgtttact aagtttatga tagggtattt
     6241 atttaaggta ataaaatatt atataaaata ttttaaagcc ttaggtaata ttaggtagaa
     6301 gttttagtaa aatatgcaaa aactaattga ttatgtacct tgtatgggta tataatagat
     6361 attgttttaa taaaatattt gaaagggaat gatttgatga aaattacttt accagaaaca
     6421 gctattgata ctttaaaaag tatcttaaaa gacaatcaag ataaacctaa taatataaga
     6481 gtatactttg caggtgttgg ttgtggaggt ccttcttttg gattagcttt agatgagaaa
     6541 aaagaagatg acttaactta tgaagttgga gaattacaat ttgtaatgag ctctgatgaa
     6601 tatagtcagt atggagatat aataatagaa gatacaggat ttggatttag agttattcct
     6661 gaaaatatga aagatcaagg tggaggagga tgctctggtt gttcagggtg ccactaagat
     6721 taagttttta gtaagtttag aactctcttt atagaaataa atttctattt taagagagtt
     6781 ctttttattt tttggtgaga taatgtataa taaaaatgta ttttactaat tttttatatg
     6841 taaggagaat tattgtgaga agaagacgaa aaaaaggtgc agatgaaaag cttttaagtt
     6901 atacaaaata tgttttaaga gacgatatag acaaattaaa aggcaagtgg aatttaaagt
     6961 ttagaaatga taatccaata catgttgaat ttgggactgg aaaaggtaag tttataacaa
     7021 ctttagctaa gcagaaccct gacataaatt atattgccat ggaacttaag gaagaagttc
     7081 ttttaaaagc tgttgaaaaa gcagatgctt ctaatttgaa taatatacta tttttatggg
     7141 gagatgtaag caatattttg gattactttg aagctaaaga attatcgaga atttatataa
     7201 atttctgtga cccatggcct aaaaatagat ggagtaaaag aagactcact cattctggat
     7261 ttttagaaat gtacaatagg gttttggaag atgatggcga aattcacttt aaaacagata
     7321 atgaaaaatt attcgagttt agtttaaatg aaattgctgc aaataattgg ctattaaaga
     7381 atatttctct tgatttagga aatagtgagt atgaaaataa tgttacaaca gagtatgaag
     7441 acaaatttat gtcacaaggt atgcgaatat ttagatgtga agctaaaaaa cgtaactagt
     7501 aatatgttta taagtttaat agtagaaatt tattttaatt tatattaata aagtgtacga
     7561 aagtaggtat atataacaag gaaacagaac cctctcatag aaattaaatt attataataa
     7621 aaaatttatg tgggagggta ctatgaattt agctaattta ataccattta ttatttctgc
     7681 agcagcttta agtccaataa attcaaatga taaggttttg agcgaaaatc aacttaaaga
     7741 tatttttcct gatgcaaatt taagagcagt tgtaaaaaga tatataaacc cagatgagat
     7801 gactataagc aatataaagg ctttagatgg agaattttat gcaactggtg agagcatttc
     7861 aaatttaaaa ggaatatcat atcttgaaaa tgtagacaat tttatttttt ggaataataa
     7921 tataaaagag gttcctaagg aagctttaag tctaaaagat atggattcaa taaatcttgc
     7981 caataattat ttgattgatg acgatgtagt aaattctctt tctcataatg gtgtggatgt
     8041 aaattgcgat ttaaatttta ttgatacaaa agataatcag tataaactgg actctaaata
     8101 tcataatgtt gatatagcaa aaggtgaaag tattgatttg agaaaaataa ttacgaagaa
     8161 aattgatagt tattataaat actgggaagt tactgataat cttccaaaag atttagactt
     8221 tattgtatct gtaagtgata agagtgtact ttcatgtgaa gatatgataa ttaaaggtaa
     8281 taaaacaggt caagctgtgg taaaagttat actaagcgac aaaaatgatt taaatacatc
     8341 tcaagaagtt ctaatcactg tcaatgtaaa ataatatgtc aacttattca atcattctat
     8401 tttcaattta agtgtataat caaaataaag gttgttagta acagaacgct aatattagtg
     8461 ctttgttact agcaaccttt aaattttata gaaatagatt atctaaaaaa ttccatttct
     8521 tctgttagta gcccatattc ctattaaaat tatcagtgaa tatattatat acaaaactat
     8581 tcttatatat tggacatcta atccaaaaaa ttcaatgtta ctctgtgagg tatgcaacat
     8641 cacaaggact gcagatatac caataagcaa aggattgatt actatttgct tatcagttct
     8701 taaaatcatt attttaccca tccaaattat tgataatata aaaaataaaa ttttaagacc
     8761 aaattctaat gtatctacca aagcgaaaac atcccttcat ttataataaa tttttcagct
     8821 caacaaaata taaaacccta caaacaactt ttgaatccat aataagataa cttgattaag
     8881 cactatgaaa taagtactat aaaacttcat ctacattaat atatactaca ctatacttca
     8941 aatttcttaa acataaatta atgaaattaa caaaatttta ttcagaaaca tataaagata
     9001 tattttacta tttaaatgca tatcattata tatacagttt taggaggtga ggtatatgga
     9061 atctaaattt tttaattggt acactcaatc cttaggggga acttttggca ttatatcatg
     9121 tatatgcgca tatttaaatg gatacatgtt tgtttataca aatagtgcta gcaaatattt
     9181 tgaaagtata ggatttctgg gaataatttc aagttataca ctacttccac tttgtatgct
     9241 tactttgatt tttggagtta tatattctta tactccagaa acatccgaca aaagtatttt
     9301 taatatacct ttttttactt taaataaaag attaatactt attacagtag tttttggttt
     9361 tataggtgct agagtttact ttataattcc tgcaattcta ataactttta acctatatag
     9421 taatgttttg atagctaaat taaagtacat cctatgtaaa aataaagaaa atgatactaa
     9481 tttgaaagaa agcgaagtct aaattgcata atattggtat ttgtcttaaa aattcccgag
     9541 gtattaattt gtattctctt g
//
LOCUS       DAEPOG010000144         9519 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEPOG010000144 DAEPOG010000000
VERSION     DAEPOG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.144"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            175..786
                     /locus_tag="KR432_002865"
     CDS             175..786
                     /locus_tag="KR432_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641383.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            814..996
                     /locus_tag="KR432_002866"
     CDS             814..996
                     /locus_tag="KR432_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641384.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1236..1778
                     /locus_tag="KR432_002867"
     CDS             1236..1778
                     /locus_tag="KR432_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0641385.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1810..2472
                     /locus_tag="KR432_002868"
     CDS             1810..2472
                     /locus_tag="KR432_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBH0641386.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2662..2979
                     /locus_tag="KR432_002869"
     CDS             2662..2979
                     /locus_tag="KR432_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641387.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3001..3444
                     /locus_tag="KR432_002870"
     CDS             3001..3444
                     /locus_tag="KR432_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641388.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3662..4105
                     /locus_tag="KR432_002871"
     CDS             3662..4105
                     /locus_tag="KR432_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641389.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4236..4656)
                     /locus_tag="KR432_002872"
                     /pseudo
     CDS             complement(4236..4656)
                     /locus_tag="KR432_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4634..5911)
                     /locus_tag="KR432_002873"
                     /pseudo
     CDS             complement(4634..5911)
                     /locus_tag="KR432_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(5934..6476)
                     /locus_tag="KR432_002874"
     CDS             complement(5934..6476)
                     /locus_tag="KR432_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641390.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6516..6734)
                     /locus_tag="KR432_002875"
     CDS             complement(6516..6734)
                     /locus_tag="KR432_002875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641391.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(6930..7673)
                     /locus_tag="KR432_002876"
     CDS             complement(6930..7673)
                     /locus_tag="KR432_002876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641392.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(7973..8530)
                     /locus_tag="KR432_002877"
     CDS             complement(7973..8530)
                     /locus_tag="KR432_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0641393.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8725..8913)
                     /locus_tag="KR432_002878"
     CDS             complement(8725..8913)
                     /locus_tag="KR432_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641394.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(8924..9499)
                     /locus_tag="KR432_002879"
     CDS             complement(8924..9499)
                     /locus_tag="KR432_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641395.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYSLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
BASE COUNT         3368 a         1285 c         1225 g         3641 t
ORIGIN      
        1 aatataataa tattaaaaac acttctctca aatcaagtat cttttgttat attttcataa
       61 gttttaaaaa tataaatttt ttaaaacttg tgtccaaaaa acaaaattaa atatgttata
      121 tatgtaaagg gttaatttta aaaccttaaa tttattatag ttagggggat ttaaatgaat
      181 atcaaaagaa ttaatctcct tttatcaaca gtgatggcag ctgtgataat atctttacca
      241 cttgctgtta cagcaaatgc aatggaaaaa acagaatcag ataataatgt gaaaatagag
      301 ttgtctgaca aagaagtaga ggaattacat aattactggc ctgaggataa aattttttca
      361 gatgaaaaga ttgatgagat cttaattaaa acaggtcaat ttactcaaaa ggaattggat
      421 aaaatcaatc aagaagtgga cgattctgct aagaaagtaa atcaaaattc tcgtgtgaaa
      481 tatataaatg ggtataacaa atacagaact tttactaaaa atggtaaaaa ctatttatat
      541 atttatgtaa gtggaaagac attaaagaaa ataaaagctg gaacagatct tgcatctact
      601 tttggaggtt ttttaccaaa aatttgggta gagcttgatg tagtggcaat tggaaagata
      661 ataacaatta acatggatgg tataaatact aattatggga ttgttttatc atatatagag
      721 gataagtgga attttcaggg tgcaacatat acttatagat atgatcattg gttctatcaa
      781 acttaaaaga catgcttttt taggaggaat aatatgaaaa aaaggcttta taaggttttt
      841 ttaaaaacaa ttccaaacct gataattgtg atattatcac ttgcattatc attttatact
      901 aatacatttg atattgattt aaaaatattg cttttaatac ctttagcata tgtacttttg
      961 tttatatact attacatcaa aacaaaaaaa gattaatttt tatggaatta gatatatacg
     1021 aaaataatta atttaaaaat ttaactttga gaatcatttg tagagttaag gcccttataa
     1081 tcccgaaaat ggatttgaag ggccttttta ctagatatct ttaaaaagct tgttaagcat
     1141 agttatattc tatttaagta gagttaatta tatatttatg ttataattga actttaaaat
     1201 attaattcag aatttttata ttggaggaat tctaaatgtt aatttatcta gctatacttg
     1261 aactcgaaga agataaaata aagtttgaaa aggtatatga aaagtataga caaataatgt
     1321 tttatgtagc caataaaata ttaaaggatg atcatttatc agaagatgca gtacataatg
     1381 cttttttaag aataattaaa aatatagata aaattgatga ggtagacagt ccaagaacga
     1441 aggcttttat cgttataata gtagaaagga ttgctataga cttttacaga aagagaaaaa
     1501 gagaaaaggt atctgatatt gaagaagagt ataaaaacag ggagataaat ttttctatag
     1561 aagataaagt atgtgaaagt aatttagcta tagcacttgc aaaacttaat gaaagttact
     1621 ttcaagtgct ttccttaaag ttccagtatg gtttttcaaa taaagaaata gcaaatgcat
     1681 taaatttagg ggaggaaaat gtatataaga gaattcagcg tgctagaaaa aaattaaaag
     1741 aaatattaga agaaatggag gttggaaata ttgagtaata aaaaagatga ttttgatatt
     1801 acggatgata tgcttagaga aagtatccca atagcgacaa aaattattat tgatgacctt
     1861 ctttcagatg atgaattaaa tcatgttttt tcaaaaggtt ttgagcgaaa aatgaaaaaa
     1921 ctcattaagc aacaaaaaag aagtagtctt acaaataaaa tcatttttta ttcaaaacaa
     1981 acagctattg tatttttaat agttttagct agtttactta ctattactat gagtagtgag
     2041 gcattgcgtt cacgtttctt tagattagta ataagagtat atgaagactt aacttcattt
     2101 gttttttcaa ctaatggaga agataatctt agtttaaaaa ataaagaacc tgagtatata
     2161 ccaaaaggat ttaaagaagt tgatagagtt gatgattttg ttattacata tagaaatgat
     2221 gaaaatattg agatacaata tgtaacagat aaaattagtt caaattctat tatattagat
     2281 acagaaaatg caaaagttaa agatatattt gtaaatgggc ataaagcaaa atatatagta
     2341 aaaggaactt tcttacagtt attttggaat gacaacaact ttatatattt attaaatgta
     2401 gattatcaaa atagtggaga atctgaattt tatgaagata ttttgataaa aatagcgaaa
     2461 agtataaaat aattttttta agtgtccaag ggtagcctct tcattcgtta tacaagtata
     2521 tatgaaaatg tgaggtgatt tttatggttt taaaatctac atttacctga gcgttaaaaa
     2581 tattgttata ggaaaaatta atacattata gttataacat gcttgcaata ggaataaaac
     2641 tttaaaattg aggtgtaaaa aatgaaatca gatttatatt tatgtaaaag taataaattg
     2701 tatagagaca acgaagttct agaaaaattt atggaagatt taagttttct tatagacgat
     2761 tataaattct cgttagaaac aatatctatt ctatgcaaaa taaatattga gaaattaact
     2821 aatttctatg caggaaaagg atatatactg tatgaagaaa tgtctcttat tgaaagtgta
     2881 cttgcacctt ttttaagtgc aattgatatt gctaaacata attatgatgt tgtcattaaa
     2941 tcgagggaaa gcgataaacc tttgatattc gaaaattaat ttaaaactgg gggaatacac
     3001 atgaaaagat tatttgcgtt attacttact ttaagtttag ttatattacc atcaacttta
     3061 gtatttgcta atgaaaaagc tgaaaatgat tcaatttatg ctgaggcgcc attagtgttt
     3121 caaagtaatg aaaaagcata tacacatggt agtggaggaa ctgcatatat aggttatatg
     3181 tcaagtaaac atcttaattg gaggataaca tcttcaaaag gaatagttat actattttca
     3241 ggaactgttg agattagaaa ttcaaaaaat atgcttgtag catcatatcc aataagtggt
     3301 ggaggatctt caactatatc tggacaaata gatgttaaat atcttaaaaa aggggattat
     3361 aaagctatat tgaaaggtac aagtacaaca ggaggcggag tcttagtagt aaaatctggt
     3421 ctagttcaga tgtttacaat ttaatattat aagtaaaata aaaatcatga atttattggg
     3481 ggatagtcac aatacatatt atttacagat attcataact attatagtta aagacattct
     3541 attataaaga aaatgtataa tttaaaatta taataaaaaa aattatcatt agtgtccaaa
     3601 aatcacttct tcattcgtta tataagttat ataaatagaa aacaaatgaa gaggtgattt
     3661 tatgaatctt agaaaaacag ttataagtgg tatgtgtgct ttagtatgtt catcagcagt
     3721 tgcaatacct gcatttgctg atgaatatca agataagaat attgaacaat attcagcagt
     3781 aaactatata gatgatacta tggtgaaaat gtcttatatt aagtctggta cttgcaattt
     3841 aagtatttca ggaagtacag ctaaagtaac atgctttgtt agaggagcat caaatgttac
     3901 aagcacatca attattgcta gattacagaa aagcaatgat aatgggaaaa catggtcaac
     3961 aatccaaaca tggaatgctt caggaaatat ttcatgttct ctttctaagt caaaaactat
     4021 aggaaaagga tattacagag ttcattccac aataaaagct aactctgaaa gtaaaaatat
     4081 aataagtagt acaaagaaat attaaaaata gatattgtat ttatgagttg tagtaaacaa
     4141 gaataatagt tttttaaaag caaatataat taattttaaa tagagtgcca atataaaata
     4201 ggtgtataat atttatactt ttttatattg gcactctatt ttataaaata agttattata
     4261 gaaaatgaaa cataatttat atataaattt tctgtttgac ctggtatata atcttttata
     4321 tcacatatca gtattttccc atcattataa ccccaaccaa tcacagttgc aggtatcata
     4381 tcagcttctc catcataaat gattgtatgt ttatacatga tttttccctc gctattatct
     4441 atagttttat tcaaaatacc ttctgctatt aatttagcaa ctatgtcttt aatagtctgt
     4501 atctgcttta ctgtctacaa aacatacttc aattaatatc gctggagctt ttgtatgact
     4561 aagccaatac aattccctta cgtccgattt tgcacctcta ttttcagcag atttatatat
     4621 atcttcagtc aatttagtta taatcttttg attcatctaa taaaatctct ccctctgaaa
     4681 ctatttcctg ttctttttta gccttttcca tttctgcatg aggattatca ataaaacttg
     4741 gttgagataa tccggtttca gtagataatt tatctccaag ttgacttaat atatttgcta
     4801 caactaaatc attatgtggt atcttagggg taaatttagc cttaatatct ttataatcat
     4861 agtcaaaacc atatttttta ttaagccaaa tgcacaataa tttgtaccta ttaaataaag
     4921 tatctgcaat actatcatta ttctggctac aaatccattc tgtactgatt aatcttgcta
     4981 caactgctaa acttgatgta ttactttgca ttttttcatt gtgatttata tgagatgtaa
     5041 ttttatatat gttttctttt attgaagata gtgtattttg aacaaatgta tcattcatgt
     5101 ctttaataag ccattctatt tttccatctc cattaacttg aagtattcca agttctttca
     5161 tccttggcaa atcatcttct ttaatattac attcagagaa ggttaaataa gcatttctaa
     5221 aatcactaat ttcattactt atatcactta aattagtttc ataagcatct tgtaatcctt
     5281 ttatatcatt gaatattgta ttttttccta gttcttcact tcttttacaa attgatattg
     5341 gaacattacc attaaatata ttatttactg taggtttttc tatctcttta aaatttgaat
     5401 caaaatgata tatgtattct ttatcatata catctatata aatatcatct ttaaaatctt
     5461 ttttaaattc tctaaaaaaa tagatggtct ctccatcttc attttcataa tggcagccat
     5521 ctgttggttt tattattctt actttcattt cattgttttt tacatagtaa agttcatagc
     5581 aaaatccaaa taatagtgta tatctcaata aatctgaatc gtgcttttta ctccaatggc
     5641 aagtatagta ttctaaatca tttataatct tctcatcacc taatctactt gtataggtga
     5701 ttttatttgc aagagaataa gcgacctctt cattaataaa cttttttaag aaattagtgt
     5761 ttatcttatt attagacctc ttggtaacca ttttatagtt actaatagca tctgtttcac
     5821 ctttataata cttatacata gtgttgtatt cttgcttttt taaactctag tcttcatata
     5881 tttctcttaa taaacttaaa tctatttcca tttaatgcat cacctccata caatcaaaga
     5941 cctaaatttc ttctatctaa tagtgtaata ctttcattta ctttaatatt aaaaatccta
     6001 tttataaatt ctgctgatat atctggtgca tcatcatgta gagagtactt ttgtcctgta
     6061 aaattaagga tttgctgtat aaatgcctta tcctcttctg caaaaatcat ttgaccttta
     6121 tttaaaacag gtattaaagt agagatttta tcatctttat ttttcttttg gtgctcatta
     6181 atgatttcta tatctctgta gtaaagaaca tcatcatttt ttattttaag ttctaattga
     6241 ttagcagctg taccattaaa tgtatttttt tctatataaa catgtgttat atctggatac
     6301 acttttaata aataaatcat atgcaatata tatttatcaa aatcagttct agcatttatt
     6361 ttggctagtt ctgccaatct gccacatagt aaaccatttt ctgattcact tccaacaaga
     6421 aaagcactat agtcatgttt agaaccacct gcagatgctg ggtcacataa taacattgtc
     6481 tttttgaatg tatgagtttc tatacattct ctagattaag ttctgattgt tttaaaccat
     6541 ttctcaccta ttgaatttac atcattttgt acctcttgtt taaaagatgt tgggttttcg
     6601 taataactta aagccatgtc taaacaattc caaaaacttg gccataatat aggaaattta
     6661 atttcttttt cattttccca gtaaaattct ttagcatctt caagatgatt tgtgtttttg
     6721 aaatcaaata acaactcttt gaaattaagc cataaaccag aattaaataa ttcatctaca
     6781 ttatcaacta aaactccttt ttcacattta aatttccaag ttggtaaatt ctttagccta
     6841 ctatagaaac attctttatg ttgtaaggtt cctaatgcta taaaagttgt tcctcttttt
     6901 ataatcttac catttcttat tactggtttt taagcagcat attttacatc atcagaaaat
     6961 tttttaaatt ttttctctct agcatcttct gttctaacat tttcttctga ctgataatca
     7021 tcaagtatga taagatctgg acgattatta ttgtattttt ttcctctcat tggtgatgaa
     7081 gatgatattg cttcaataaa ggttttatta gttaattcta attgagtact attgcaaata
     7141 tattttctat cattatcatt taagagaact ccaaatgctt ttttaatata ctcattttca
     7201 agtaatgcat tttttatatc ttttacgaat ttttctgctg tagagcctat atcagagcaa
     7261 attaaagtat atgtcttatg tttataacaa tgtgaccata cagttgttgc taaatctcca
     7321 aatgcacttt tacctgttcc tcttggataa attcttccta attgctgtga accatctcca
     7381 attatagact cttgtatatc ttcccataac tcatgatgaa ctttagctat aggagcagca
     7441 acattatctt cttttggtaa atatgtgtct tgtataaaat acatacagaa aaattctaaa
     7501 tttatttttc caagctgata agctaggcca ttaactcccc aaagattgct tgaattagtt
     7561 tttataatct catttgcttc tttttcagct tctcttatag ttgctccatt tcttataaag
     7621 gtctttgcca tataactaaa tattaattgt atatttcttt ctaaattatc cactcgtatt
     7681 tacttcctct taattcttct atttttttag gttcagtgag taatacaatt aataaaataa
     7741 acaatgcaaa tttttttcta ataaaatcac cctctttgaa atatgtctct ggtttttaat
     7801 ttttgctaga aaatttctag aactcatttt ctataaataa tacttttttg aaatagaagg
     7861 atacccccta tactgaaaaa cagttattta taattatttt aaacataatt aaagggcata
     7921 attctatgaa tttgccccac taaaattaac ttaagcatat atattgctct tcttagttac
     7981 tctcatggct ctttattaat ctatctaatg ttggtctact tactcccaat tctttagcca
     8041 attgagtctt attaagttct ctagttttat atctatcata ataactacca aagttgtcta
     8101 tcttaacttc tttacgtcct ttatatttac cctgttcttt agctatagag ataccttctc
     8161 gttgtctttc taacatgttt gttctctcaa attcatatat tgcgccaagc attgtaagca
     8221 taagttttcc tgtaggtgta cttgtatcta aattttcttt atcactaatt aactttacat
     8281 gtttcttttc taagagctca actatttcta ataagtcttt agtacttcta gcaagtctac
     8341 taaagtcatg tacaattatt gtatctcctt ctcttacaaa atctaacatt gcgtttagtt
     8401 ttgatctttt agtatctttg gcacttattt tctcctgata cattttttct acattatgct
     8461 ttttcattgt aactaattgt ctatcttcat tttgttctgc tgttgaaact ctcatataac
     8521 ctactaccat ctttgatacc tccacctatg tttactctat aaataatatt atagcataaa
     8581 tgtaaaacta gagttaaaga tttgccatgc tttttgtaaa ttaattggag ttttgatttt
     8641 atttttacat cagaatagat agtttgtaat gtaaatttgt accagcatac tttacaatat
     8701 gctcttagga aagatatttc ttgtttatag attataatat ttctctagta tctttatttt
     8761 tataatttca aaaataaaaa ttaaaattac agttattatc atatttcgtg tactattttc
     8821 tataatatta taactaaaca atgcaaaaac aatgattaat aaaattacgt ttatagtgcc
     8881 agaaatcaag aatgattttt tcttattatt catatatttc ctcctagtta tttgctagtt
     8941 ttataccatc tcctactctt tcagtatata caactttttt aaagttagat gagctataaa
     9001 aatatgtaac atattcatat ctgtatggaa caggaacatt agcatgactg tttctaatat
     9061 cagttatttg tctcctatag tatgtttttg tatatacgcc tccatattta gtaatgtcat
     9121 atactttttc tctataaata ctctcagcta aacctaataa aaaagatgac cctccagaaa
     9181 gataacctaa tccccattga atagtttgta taattctttc tcttgtataa ggagaactta
     9241 atttgcgtga tttttgacct agatatttaa catttttcca actaccaggt gtaacttttg
     9301 ttgaaacata agtgttattg ccttttttat ccacaatcat ttcattttca ttgctattaa
     9361 taatagtaaa ttcgttttca ttaacaagtt cttgtttata ttcattttct tcaaaagtag
     9421 atgcataaac caaactatat ccactagata aaacaaatat tcctgccatt aaaaaactta
     9481 ttattttttt ctttaacata atattttact acctttact
//
LOCUS       DAEPOG010000145         9517 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEPOG010000145 DAEPOG010000000
VERSION     DAEPOG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.145"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     misc_binding    171..382
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            545..1834
                     /locus_tag="KR432_002880"
     CDS             545..1834
                     /locus_tag="KR432_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0641396.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1888..2967
                     /locus_tag="KR432_002881"
     CDS             1888..2967
                     /locus_tag="KR432_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBH0641397.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2988..4532
                     /locus_tag="KR432_002882"
     CDS             2988..4532
                     /locus_tag="KR432_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBH0641398.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4695..5237)
                     /locus_tag="KR432_002883"
     CDS             complement(4695..5237)
                     /locus_tag="KR432_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBH0641399.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5385..5669)
                     /locus_tag="KR432_002884"
     CDS             complement(5385..5669)
                     /locus_tag="KR432_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0641400.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5732..6496)
                     /locus_tag="KR432_002885"
     CDS             complement(5732..6496)
                     /locus_tag="KR432_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0641401.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6778..7044)
                     /locus_tag="KR432_002886"
     CDS             complement(6778..7044)
                     /locus_tag="KR432_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0641402.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7087..7896)
                     /locus_tag="KR432_002887"
     CDS             complement(7087..7896)
                     /locus_tag="KR432_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641403.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8209..8490
                     /locus_tag="KR432_002888"
     CDS             8209..8490
                     /locus_tag="KR432_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641404.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            8754..9008
                     /locus_tag="KR432_002889"
     CDS             8754..9008
                     /locus_tag="KR432_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641405.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            9053..9376
                     /locus_tag="KR432_002890"
     CDS             9053..9376
                     /locus_tag="KR432_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641406.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT         3454 a         1116 c         1530 g         3417 t
ORIGIN      
        1 ataaaaaaaa tatattttga tgaaatttaa aaaaatacat tatataaata tagtaatagt
       61 atgaaaattt acatataaaa ctaaaaaatg taagtaattg actttttaag ttgacaaaat
      121 attgcatata tattatgata taaatcataa attaactaca aatcaatatt atatattcgt
      181 agaagaagaa agtagcataa aggaatttta cagagagtta gatttggctg agaactaata
      241 ttggaattta tgtgaaaagg actttggaga atactattgt aattatgtta gaatagtggt
      301 ttaattcgcc ttaagaaaat gagtgagtaa aattaatttt tactaataag agtggtaaca
      361 cggatttata ttcgtctctt ggatttttcc aagaggcgtt ttttattata aacttatttc
      421 agaaaattta aaactatata ttaaatgaaa attataaaaa tatgatagtt aataaattta
      481 taaattagaa aattgtaaaa tatgctaagg tcagtagttt tacgatttaa atggagggat
      541 gaaaatggaa gaagaaaaaa ttaactatgc tctggaacag attccagaga atcaaaaacg
      601 tggttgggta gctatgtttt cagttttagt agcaataggt gtggatttat catctgttat
      661 tttaggcgct gagttggcac agagtatgcc aatgaaacaa gcaatattgt cagtaattgt
      721 tggttcattt ttttctgcaa ttttgtatac tacatgctct ttggttggtt catctacaag
      781 tttatctaca tctatgataa caaagtatgt atttggagaa gctggagcaa aaatattttc
      841 tctagttata ggggtttctc tattaggttg gtttggagtt caagtaggtt tctttgcaca
      901 aaatgcacag ataataataa aagatatatt taatttagat gtcagtatgc agattttaag
      961 cctaataggt ggtctcttga tgatgagtac tgcaatttat ggatataagg ccatggagaa
     1021 gttgagtgtg tattcagtac catttttatt agttcttatg atgctaacta tattcttagc
     1081 ttttagagca aatggaattt cagttgatga taatatgaaa tctactatga catttgcagg
     1141 tggagtttct ctgtcaatga gtataataat agttggtgca attgtatctc ctgatataag
     1201 tcgttgggct aaaagtagaa gagactgtgc tctttcaagc tttttgggaa tacaatttgg
     1261 aaatgctttt atgataatag tttcgatagt attagtaaaa tgtatgggaa cttcagatat
     1321 tatgagaatt tttattacat taggaattgc tataccaggt ataatagtac ttacattagc
     1381 acaatggact acaaatacaa gtaatgtata ttcagcatca ttaagtatag ctcttgtact
     1441 taagaaggct cctgagaaag tattaactat agtccttggt attatagcaa cattattggc
     1501 tgtatttgga atatatgaag gttttatagg atttttaaat ttacttggaa ttgtaattgc
     1561 acctgttggt ggtgtatata cagcagaata ttacatagta aagcaagaac ttaaaggctt
     1621 tgataaagga gtattatata aaccaatagt taaacgttca atagtgtctt ggataattgg
     1681 tatattaata acttaccttt caacatatgg atttataact cttactacta tagcaccttt
     1741 agatggtttt atagcagggt ttgtagtaca atctattatt ggaaaagtgc tttgttcaac
     1801 aaaaaataag caagaaatag acaaagcagt ataaagaaat ttgtttataa agtataaaat
     1861 aaattagaaa tatgaggaga aagtaacatg agatttttaa cagaagaaag tgtagataaa
     1921 atagcagttg gtgcagcagt gcttggaact ggaggtggag gtgacccata tgtaggtaaa
     1981 ttggttgcaa aacaagccat taaaaagtat ggtcctgtaa aagtaataag tttagatgaa
     2041 cttgatgatg atgctttggt agttccagta tctggtatgg gttctcctgt aattacaata
     2101 gaaaaattac tttcagaagt agagttaact acccctttag aaataatgga gaaattatta
     2161 aatagaaaag tggatgttat aattcctata gaaataggag ggataaactc acttatgcct
     2221 atagcagttg ctgctaaaaa aggacttcct atcttagatg cagattctat gggtagagct
     2281 ttccctgaag ctcaaatggt aactttttat cttgaaggat atgaggcatc tcctgccgta
     2341 atgtctgatg aaaaaggtaa ttctgccatc ctttatcctg tggatggaat ttggtcagaa
     2401 agattagcta gaactttaac tgtagaaatg ggaggaagtt cttctattag tgactataat
     2461 ttaagtggag ctcaagttaa aaaagctgct ataggtaata ctcttacaat agctgaaact
     2521 ataggagggt ttttacttga aaataaatct gattcaaaag gtgctgttga gaatatacta
     2581 aaagaattgt gtggatacaa gttatttgaa ggaaaagtaa ttgatattaa gagagagttg
     2641 aaaggtggat ttactagagg acatgccttt tttgctggga ttaatgagta tgatggagag
     2701 tattctatat tattccaaaa tgagaattta attgccaaaa aaggtgatac accactttgt
     2761 attacaccag atttaatagc agtattagat ttagaaactg gatttccaat aactacagag
     2821 agaataaaat atggctctcg tgttatggta gtagcttttc cttgtaatga aaagtggaga
     2881 acagaaaaag gaattgagac agttggacct ggatactttg gatatgatgt ggaatataaa
     2941 acagtagaag aattacaagg aaaataaaag tttaggagga aattttgatg tatcgtgtag
     3001 gaattgacgt tggaggaaca aatacagatg cttgtatact ggatggggaa ttaaaagtaa
     3061 ttcattcagt taaggtggca actacaaaag atgttgaaac tggtgtttac aatgccttaa
     3121 aaaaagttat agatgaaagt aagattgacc attcattaat aaaatatgct atgcttggta
     3181 caactcattg tacaaatgct attgtagaaa gaaaaagact taataatgta gctatgataa
     3241 gaataggaaa gccggccact actgccattc ttccatttat agattggcca gaagacttaa
     3301 gagaaaaaat agaattagat tcaatattgg tatctggtgg atatgagttt gatggtagaa
     3361 agataaatga actttgtaaa gatgaagttg tagagttttg caatagaata aaaggcaaag
     3421 tggaaagtgt tgcaataagt ggagtatttg ctccagtaaa taaacaacaa gaagaagaag
     3481 ttgcaaaatg ggttagagaa attttagggg atattcctat atctttatca aatgaaatag
     3541 gtaatatagg tatattagaa agagaaaatg gagctatttt aaattctgca ttgtctcaag
     3601 taggtaaagc tgtatcttta ggatttgaaa aagcacttaa agacttaggt atagatgctc
     3661 aaatatattt cagtcaaaat gatggaacat taatgaattt agagtataca atgaaatacc
     3721 caatttttac aattggatgt ggaataacaa attctataag aggagcttcc ttcttatcaa
     3781 aagtaaaaaa tgcaatagta ttagatgttg gtggaactac aagcgacttg ggtgtactat
     3841 ataatggatt ccctagagaa tcatctattc cagttacaat tggaggagta catacaaact
     3901 ttagaatgcc agatgtttta actattggtc ttgctggtgg tacatgtgta aaaggcgaaa
     3961 aagataatat aaagataggt cctgaaagtg tgggatatag aataactgaa gatgcaataa
     4021 tatttggtgg agacactcta actttatcag atgttgttac aaaattagat atggcgtttg
     4081 aagatagaaa aagcaatgtt gaaaacttag ataaagagta ttgtaatgag gtatataaga
     4141 aggttatttt aaaattagaa gatgctgtag accaaatgaa gactagtcaa gatgatgttg
     4201 aattggtttt aactggtgga ggaagtataa ttgttcctga aaaactaaaa ggcataagca
     4261 atgttataag acctcctcat tatactgctg caaatgctat tggtgcagct ttaggacaaa
     4321 taagtggaga tgtagaaaaa gtatacagtt tagataaaat gtcgagagaa gatgccatag
     4381 aagatgcaaa aaaagaggct gtaaataaag ctctaaaagc tggtgctaaa gaagatacta
     4441 tagaagtatt aaatatagaa gatgttccac ttgcatacct tcctggtaat gcacttctta
     4501 taaaagtaaa agttgttggt gatttaattt aaagaataaa tataagtatt tccagaatta
     4561 tataaatatt tctggactca tttttaaact tattttatca ataaaaacag cataactttt
     4621 gactatattc ttataagttt ccgaacagcc gatatatacc tattttttga ggtttatcgg
     4681 ctgtttgtta tcttttatcg cattccaact atttttctta ccgtatttgc tgtacgaatt
     4741 gtaatcttat cacttacatt tgaactggca gtctttgacc accaatttgt ctttttataa
     4801 tctttacgac taaaagacca aaagatagca tttttataat gatatacttt ttcatactct
     4861 tctttaggat ttccaacctc atcataaaac tcgtcataag ataagtcagg aaacataaaa
     4921 ataagattat catatatttc tttgttgtta tctccccacc aattaggagc attatttagt
     4981 atatctttta actcctcttg tgaaataata aaaactggta tttccaattc aaactgtttt
     5041 tttatcatta atcttattgt atttgagata accttgttgt catctacatc acttaaaaag
     5101 acaacattac cactattaag gtatgtagaa acttctgaat aaccaagttc tacaaagctt
     5161 gcttttaatt cagacataat tatcttgttc ttaccactta tattgatacc tcttaataat
     5221 gcgatataac ttttcatata ataagttcac ctctaaacat tcacattttt ctatgtagta
     5281 caaactggaa tttgcatctt ttataattct ttcaaaactt cttcatatgt aattttttta
     5341 atataaaaaa atcggtgctt aatttgtacc gatttttatt tttcttagta aagaactcgt
     5401 ttttttgctt ctgctatagc tctagcagca ctttttgctt gcattctttt ttcttcttct
     5461 tttttaaatt catttattgt ttcatttaga ttcttatcat gttcattttt ttctttatct
     5521 tcgttttctc tgtatactaa tatataatct attattgatt cgtttattat ctgagatata
     5581 cttttatttt cattaattcc tattctctta acttccttaa gtagagtctc atcaatatga
     5641 accgttgttt gctttgtatt ttttgccacc ttaatcacct cttaaaaaaa attctaacat
     5701 taaaattttt tttagtcaat aaattttaat attaaaattc tttaaatgtt ttatttgatt
     5761 ttctaaaatt ttctattgta tttatcatct tattttttat attttcacct agtacagaag
     5821 actttagtct aggattttct atatggtcat aaattggttc taaaaattcg acttcgacag
     5881 gaacagagtt aattttacca ataggttggt atccttcata agtatctttt gaatttttta
     5941 ttacgagtgg aacaataggg cattttgctt tatatgctat ctttaaagct ccacttctaa
     6001 actccgaaac caatgaattt ggttctttaa tccatgttaa atctccttct ggaaacactg
     6061 ccatactttg acctgtaagt atattttgag aggcttgggc tatactcttt ataccctcac
     6121 gattgttttt tctgtttaca tatacacatc ttagtaattt tgcccattct ttaaatatag
     6181 gtatatttct ccaaactggt tcatctgcaa ttacacatcc aattggtctt tctacacttg
     6241 ctgttagtat gaagctgtca agcatactag aatgatttac aacaaataga agtggttctt
     6301 ttggcagtgt ttccttacca attatattaa gctctatgtt cactatatct aaagattttt
     6361 tagcttgttt ttgcataaat tcaaattttt cctttaagct aaatttatca ggattctttt
     6421 taattttaat aaggctaggt atagaagatg taaaacctat aagttgatat gtagcaaatt
     6481 ttaagtaatt attcataaaa ttatctcctt aaaataaaat tatttattac tgtaaagatt
     6541 ttaacataaa ataaaattta agtctatttt aattcaattt tgtaacatat attttaaata
     6601 gacagagatt gagattttat atttctaatt ttagtaatca gattttatca ataaattact
     6661 attctattaa tttaattaaa taacctttaa atttaattat aaaaactgtt gcagaaattt
     6721 ctacaacagt ttaaaaaatt taccttacaa aaacttattt tataacaaga gagtatatta
     6781 taaggctagt acagcccatt ttttgcactg agaaattctt ttgccatcat caccttgttc
     6841 tttgtcatat gcaaattgat taagcatttc acatggaaat gtatcacata gaccacaatg
     6901 tataaggttt ttatcttcac aacaagattt tacaggacat tgttcacccc aaaatggttt
     6961 ctcaatatct gtacaacctt tacaattgac tttttccttg tatccacatt cataacataa
     7021 aattccacat cttgattcta tcattttaag tcactcctta aatataaatt ttattatgat
     7081 tgtatatcaa aatttatact tttcattgta aaaatcggac aaatttttta tataggttgt
     7141 tggattaaca ccacaaatag atttaaaatc atttataaaa tgagattggt cgtaataacc
     7201 aatatcttgt gctaaagttg caaatggtct tttattgttt tgtacttctt gtaatacaag
     7261 gtttatacga agtaatctaa ggtatgattt aacactcatc ccaagagaat tattaaaaat
     7321 tctatttaaa tgtcgttcac tataacaact aatttgtgaa atgtttttaa cagacataat
     7381 agagttctgt tttctagcat tttcaagaat tggtatagtc atatctacta tatttgattt
     7441 caataagtat gataagaata acatatctaa ttgttctact aattccttta tagtacttgt
     7501 tctttcaaaa atggaattaa cctctaagct aaataatgtg ttaaaatctt caagtgaaat
     7561 ttttaaatct gtagattctc tttgagaaag acctgtcaaa taatatactc cacctgggcg
     7621 aaactcaacg aatactctaa aaagtacatt ttctatatca ttttttacaa tagtagtttt
     7681 tgttgtggct ccccagaatg cgctttctat accttttttg tcaaatttaa atatcataca
     7741 tccactagca tcaggtatta atgtaagctt ttctttaata cttgtatctg gaatagatat
     7801 tgtatagtgt gctatatact gtcttaacaa tttatgtggc aaaatatatg tatactgttt
     7861 gcaggtattt aaaatgttat tgattatttt attcataagt atatcctcct gatatataaa
     7921 aaataagtat actaatttat tttttatata ataaaccagt tttgttttaa ttgaaatatt
     7981 ctaataagat attttgttaa atttagatac aataattgta aagtaaaatc tcaaaatata
     8041 caattattat aaatcataga tatttattaa ataaatatat taaagaagaa ggaatataaa
     8101 acaagattag aaaataatag tgttactttc ataataaata catataaaga tatttttaag
     8161 aataaaattt attattaaat aaaaaataaa ggagagtgat ttaatcgtat ggcatcaaaa
     8221 caaacaccaa gaaacaattt agaaaagttt ttaagaataa gagtcttatc acatacaggc
     8281 gataaaatac atataaagtt acctgtaaat tttgcaaaaa gaatgattga gaataatgct
     8341 ttggatttat ttaatggaaa agatgatgta gtagatggta aaaaggttac tgaaatatta
     8401 gtcaaagcat ttgactatga tttaactgga gaagtagttc acttggagag aaaaaatgga
     8461 gatattatta aagtttcaat tgtacaatag aattatttta tatgaatttt atttttgaat
     8521 agttataaat aattagaaat ttgtaaaaaa gtataatata ttattagtaa tggtgcagaa
     8581 tacacattta taatttatag atgtgtattt tttgtttaaa aaatttaaat atataaaact
     8641 ataaaggtta caattttgga aatagatgta aatatatgtt ctattgtata ataagagtaa
     8701 tataaaatct agtaaaaata atcaaataaa tttaacttat gagagggata actatggaaa
     8761 ataaggattt taaattaaca atatgtatat ttgcttttgt aattggagta gtagtattat
     8821 tctgctgtat aggtttgggt gaatttagtg cagagcatat tattcaacaa aatggtggaa
     8881 tcatggatac tgaccaatat aatatttatc tagaccaatc tatttctcaa tatagaaatt
     8941 ttggttctat attagctctg ttaggaggaa tagggatatt gtttgataaa tatatcagaa
     9001 aatactagag attatgtttt gtctaaaata aattcttatg aggagataaa aaatggcaac
     9061 agaaatattt actgtaaaaa ttacaccctt agaagcacaa aaaatgataa aagaaaatat
     9121 tgatgcagat ttagttttta gtgattctta taatttagga gatgataaat ttatactaat
     9181 tacaactttt gagaagtatt acgcaagaaa taatagtgat gcaggattaa tcgtagttta
     9241 tgaaaatacc acagaaaaaa cagttattaa agctacttca acaggcagtg caattggagt
     9301 tttaaaaatt gattggggtg caggtggaaa tttacttaaa agagtaaaaa acatacttgc
     9361 tgaatatatt atttaattat tttatctaac tgataatatg tcaagaaaat ctcgtaaaag
     9421 aggttaagaa tatttttata atatatagat aaataaggat ttgaaggtaa agtttttaat
     9481 gtataaagaa aaataatata ataaaacgaa ttggctt
//
LOCUS       DAEPOG010000146         9286 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEPOG010000146 DAEPOG010000000
VERSION     DAEPOG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.146"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1118
                     /locus_tag="KR432_002891"
     CDS             <1..1118
                     /locus_tag="KR432_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641407.1"
                     /translation="DNLKIKTIKGDSNELDHININFGYALNGYINSYYTINGSNKTKK
                     TELIKLQKDSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEKGVFPS
                     EGSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYANILASKRYKKYLYVSVLYATSDSSE
                     NNKEYSSTIDIIKINPENKKIKTIKSINLSNYFSEKYQDIENIGVSYASKYEDKMYIL
                     VNVLNTNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLYMVMSND
                     YDNSSITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRLNSILS
                     WDNKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1127..1939
                     /locus_tag="KR432_002892"
     CDS             1127..1939
                     /locus_tag="KR432_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641408.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1950..3218
                     /locus_tag="KR432_002893"
     CDS             1950..3218
                     /locus_tag="KR432_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641409.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3584..4765
                     /locus_tag="KR432_002894"
     CDS             3584..4765
                     /locus_tag="KR432_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0641410.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(4907..5824)
                     /locus_tag="KR432_002895"
     CDS             complement(4907..5824)
                     /locus_tag="KR432_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641411.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            6077..6871
                     /locus_tag="KR432_002896"
     CDS             6077..6871
                     /locus_tag="KR432_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641412.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            6872..8932
                     /locus_tag="KR432_002897"
     CDS             6872..8932
                     /locus_tag="KR432_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0641413.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         3725 a          907 c         1464 g         3190 t
ORIGIN      
        1 aagataattt aaagataaaa actataaaag gtgactctaa tgaattagac catataaaca
       61 taaattttgg atatgctttg aatggatata taaattcata ttatacaata aatggaagta
      121 acaaaactaa aaagactgaa ttaataaaac tacaaaagga tagtaagaac acaaataatt
      181 taattgatgt atctagtggt tcagatttaa ttgctcatgt tggatttgaa atgaatgagg
      241 agtcaaaaga tataaacttt gtgatagatg agaaaaaaga agatttagaa aaaggtgtat
      301 ttccatcaga aggtagtgat aaagaaattg aatatgaaaa attagatatg aataatttta
      361 caaggacaag atataaagta gataaaagta cagatgatta ttatgcaaat atactagcat
      421 caaaaagata taaaaaatac ttatatgtat ctgtacttta tgctacaagt gattcatcag
      481 aaaataacaa agaatattca agtactatag atataataaa aattaatcca gaaaataaaa
      541 aaatcaagac tataaaaagt ataaatttaa gtaattattt ttccgagaaa taccaagaca
      601 tagagaacat aggtgtaagt tatgctagca aatatgagga taagatgtat attttagtaa
      661 atgtactaaa tacaaacata ggtgataata gtaattctaa atttcattta cttgagtata
      721 atcttgaaga aaataaggaa agcataatgg aattaaaaac tgatagcaaa atggctttag
      781 caaaagcaaa gttagaagct aataaattat atatggtaat gtcgaatgat tatgataata
      841 gttcaattac agagttaaat ttacttacct ataatatatt agataaaagc ttagatgaag
      901 ataaatatgt tttagataat agagaaaaaa gaggaattag aagtattgaa attgattcaa
      961 ataaaatcgt tgcatataca tcaaggctaa actctatatt atcttgggat aataaattta
     1021 tgctatatat aataaataaa aattctaagg aaattgtata tgaaggagaa ttacaattta
     1081 acagttcaaa tagtatgtta gaatttgaaa taaagtaggt gagttgatga aagatttaat
     1141 aagattattc gaatttgaat taaaaagaaa tataaagaat tatatattta taataatttg
     1201 ttgttgttct tttgtaattt taaacatagt aaaaaattta aatgactata attacataat
     1261 agaaaatgca gttaaaagtg aacagttaac aaaaatcgga aatacgatag aaacggtaaa
     1321 tgttacaggt ttctcaagct ttagaaatat aatgggttct acagaatcgt ggtttatatt
     1381 tggtataatt gcatgtattt gctatgcttt ttttatttgg tatagagact ttaatggtag
     1441 gagtaaatct atatatacac ttataatgct acctaaaaat agaatcaata tttatatatc
     1501 taaattatta aatatattat ttctagtgta tagctataca gtagtactta caatatcttt
     1561 gtttattgca agtaagttgt tgcctagaca tatgcttggt aatgtcacaa actatggttt
     1621 tgtacaagaa acaatatatg agttaaaaat gttacttcca tatagctttg aaatactatt
     1681 tgtagagtat atatttttat tgataggatt tgtatctgta gtatttacat caattcttat
     1741 aaataaatct atttataaag taagcacttt aataagtttt ctatttttag ttttggaaat
     1801 tttagtgttt ataatttcta tagaatttat tataccttat gaatacagtg atgtatttac
     1861 tgttctatat tcaagtataa acatatttgt atgtagtctt atatctaata aacttttgaa
     1921 gcaaaagatt gacttttagg agggatatta tgaaagcaaa aaatatagga aaatatacaa
     1981 tatgttttat aatagtatgt tttttaatga ttactatgac cttaatattg agttatttta
     2041 ataaggataa actatctgta aaaactttaa aaggcaatgt aaaagatata gggagagtaa
     2101 gtgtagtata tacacctaat ataagcaaat tttcttatga ggaggtaatt ataagtaaaa
     2161 atggagtaag aaataataaa aaccccaaat caagaattag aaatgattat ttaaatattg
     2221 acaaaaaata cagtgatttt gttaatgata aaggaaaaga acttatatat aaagatgatt
     2281 tgaatatagg agaagcgtat acctctgtta agtctgattt agtacaagga atgaatgctt
     2341 taaatgattg tataaaggtt tcaaataaaa atttaaaaag tggggtagaa gaaaattttg
     2401 atattaattt aaataagtct gattttaaag aaaacagttg gaaaaccttg tttaataaaa
     2461 tatataaagg caatttatat atggtaatta gtgaaactaa tatttcaggt tctaaggtca
     2521 ataatatttg tatattaaag atagatataa aaaataagtc ttataaatta ttaaatagat
     2581 ttaatttagg attacagaat ggatatcaac aaaatatcaa tgaaattaaa ttttctattg
     2641 gaagtaaatt atacttagaa atagtaaata ataaaagtaa aaatggagat attgaaggac
     2701 atgatagtta ctctaacctt attatatatg atatagaaga taacactttt aatataagta
     2761 ataaatttat ggataatcta aacaaagaaa agaatgcaaa tccaaaggag attggcttat
     2821 taagtaaaac taagctagat tatatgataa aaaataataa attgaatctt atagttacgg
     2881 atatgagtaa ttccataatc aatcttgttt atgatattaa ttctaatgag attaaattag
     2941 aagattatga gatacttgat ttaaaagtag attataatga agcatcatct ggagttagga
     3001 aagttaagtt actggatgat aagatttatt ctatttcagg tatgaagaac agtatatcag
     3061 gagaattaaa aggatttaaa gggattgttg atgtagtcgg aactaagcca gtaaaatttc
     3121 aagtatttga tatgaaactt aaaaagaata tttatgaagc tgagctaata aatggagata
     3181 taagtttaca agaaaagtta ttttttgttg caaattaagt attagaatta attttagaac
     3241 tttaaacttt tatatgtatc acagataagt ataagtaagg gagtgtctca aaataaactt
     3301 tttagttcat gagacacttt ttttatatga aatcaaatat attttcattg tttagattta
     3361 ttttgataat gaaaaaaaga ttatctttat tttaagaaag acatttattt ataattatat
     3421 acaaaaatat tttttaatgt ttttgtgtat aattataaca atttaattta tattaatgca
     3481 cttaaatttg aaatagaatg cttatagtgg taaaattaat tattagaata ttagaaataa
     3541 taaataaata atacattgta cttaaaaggg ggagaaattt aagatgaaca aaaaatatat
     3601 taaagcaggg aatttaattg atggaacagg aaatagtatt atgcaaaata agggtataat
     3661 tattgatgga gatactatag ttgaaattga agatataaga gatgatttaa atggatatga
     3721 tgtgtacgat tacttagata aaactgttat gccaggcatt atgaattgtc atgttcactt
     3781 gacaatggag ccagttggaa atccaaagat atattatgat aatgtatcag atgtagagct
     3841 tgtagtaaat acgataaaac aagttgatgc ttatttggat tcaggagtaa catatattag
     3901 aagcttaggt tgtcccaaat atgtggatgt acaacttaaa aatcttatag acaaagggtc
     3961 tatagatggt cctggtatag ttacttcagg tccagttata tgtatgacag gagggcatgg
     4021 atattatttt ggtattgaaa gtgatggagt ggatgaatgt agaaaatcag ccagaacagt
     4081 tttaaaaaat ggagtagatt gtgtaaagat aatggcaact ggaggtgtaa caactgatgg
     4141 agttgagcca gggtcaccac aattaacttt ggaggaaatg aaggctgctg tagatgaagc
     4201 tataaaagca ggtaaaataa ctgccacaca tgcacaagga agaactggta taaaaaatgc
     4261 agttcttgca ggaattactt ctatagaaca tggtgtttat ttagatgatg aaataattga
     4321 tttgatgctt gaaagaggaa cttacttagt tccaactgtg gctgcaccat attttattct
     4381 aaatgcagga aaagaaagtg gaataactga agctactctt agaaagtgtg agattgttgc
     4441 cattccacat aaagaatcat tcctaaaagc atataaaaaa ggaattaaaa ttgcagttgg
     4501 ttctgatgca ggaacttctt ataatgaaca tggaaaaaca tattatgaaa tgaagctaat
     4561 gtcagattat ggtatggata atatggatat aatagttgct gctacaaaaa cagcatctga
     4621 acttttaaga attgataaaa attatggaac tctagaaaaa ggaaagaaag cagatgttat
     4681 agttgtaaat ggaaatccag tagagaatat agacgtactt gcagatgtgt tagcagtatt
     4741 taaacttggt aaaaaagtta gataaatagt ataaaaataa aaaagagtgt ctaaagaaat
     4801 ttttaaataa ttatagacac tcttttttat tagtaataat taaatctttt atactttaga
     4861 aaatagattc tattttatat ttagatataa ctctttaaaa tcattcttat gcaatataat
     4921 ctatagaatc tacacataca aagtatattc ttgaatcatt agaatttcca agtactataa
     4981 catcattttt cttacctaga acttcaacac cagttagaac caaactacca gctactacag
     5041 taacttgatt atttatattt gacatataaa gattatctag gataccttta ttacaacagc
     5101 aatcatcaca atcgcagtta caattacatt ctttgtaatg ttttttatca agatagtaac
     5161 ctaaagatgc tatgaaatct tcataatctg caggtgtaat atcaaatgca acagctttaa
     5221 gattacacaa tgatgctaaa tctacgtttg ctaatggagt agatgcagca agtataaaat
     5281 caattatagc agcaagtatt gcatctaata tagccccaag gtctatcgta ggaggaataa
     5341 ctgcatcaat aagtgcaatt aattcagcta ctcctggtat agttcctata gtattaatta
     5401 atccctcaag agttaaaggg actggaatag gataagatac tctaccagct atatctatta
     5461 aatcacagtt acaagcagaa aatctttcaa aagtaccatc aagtccagat aaattatctt
     5521 ttggaggagc tgaaagatct atacctacca aattagcacc tactataaag aaatctgaga
     5581 taaaagcaaa ttgattaaag tttacaaaag gtcttagagc atcatatctt agaagttcta
     5641 aggcctctct catacttttt ttacaacaac attgttttat tttaggttct ttatatttat
     5701 ttggataatt atagtatcta tcagctactt gataattttc atagtatctt tcatttgtat
     5761 ttggataatc ttcctcatat tcttgtgtaa atctaaagtc ctctctacat ttattatttt
     5821 ccactaaata cactcctttt atattttaat ttaaatgtat agataattaa gcttatctat
     5881 atagtaatat tatatttata atgtaaaaaa atgttacaaa agatattata aacgaataaa
     5941 aaataataat ttgtcagata aacttgtaaa tttttatgaa taatccattc taaaacaaaa
     6001 aactgtgtat aaaaaaggtt tatatgttat atttatataa atataacata tgaacgagaa
     6061 aggagtggtt tgataaatga ttgaggtaga taaattgtgt aagtctttta caagggttgt
     6121 aaaagatgat aagaataaaa gttcaataag aaagctaaag aaagtaaaaa ctaaaaaaga
     6181 agaatttttt gctgtaaata atgtttcatt taaagttatg gaaggtgaaa ttgttggaat
     6241 attaggtcca aatggtgcag gaaagactac attacttaga atgttaggtg gcatattaac
     6301 accaacatca ggaagtataa atatttctgg atatgattat tcaatagata gaaattcagc
     6361 aaaaaaagaa ataggttatc tctctggaaa tacaaaacta tatggaaggc tttctccgag
     6421 agaacttcta acaacttttg caagtctata tgagatgtca aaagaagata tagaagaatc
     6481 aattgaaaat attgtaaaaa taatggatat gagtgaattt atagataata gaattgaaaa
     6541 tctatcaact ggtcagactc aaagaacatc tatagcccgt tgtttaatac attctccaaa
     6601 agtatatatt tttgatgagc caacactagg tctggatgtt ttaagtagtg catctattat
     6661 agattttatg aagaatgaaa aactaagagg aaaaactgtt ctttactcaa ctcattatat
     6721 ggaagaagca gaaactttat gtgacaagat ttttatgatt cacaatggga aaataattgc
     6781 atcaggaaca ccacagagtt taaaagagga atctggagta aataatttga gagatgtatt
     6841 cataaaactt gccagaaagg aggacattta aatgagaagt aagattgtaa aatacatatt
     6901 taaaaaagaa atgttagaca tattgagaga taaaaagact ttatttatga tgattgttgt
     6961 gccacttctt ttatatcctt tacttatgtt attaatgatt caagtaatga atatgtctgc
     7021 aagtagtatg aatagtaagg acattaatat agcttttaat aataaaccta ataaggtgtt
     7081 agtatccatg attgaagatg ataatttaga aaaagatact aaaaatactt caaatgataa
     7141 aggcaaaata agagtgttag atgtagataa ctataaaaag tcattagaag aaaataaaat
     7201 tgatgcttat ataaagatgg aagaaaaaaa atcatctata aattatcaaa tatatataaa
     7261 ttcttctcaa gaaaattctt ctaatgcact aaaaagaatt gaaactgttt tggataaata
     7321 taaaagattt accatagaaa aaactttatc tgaaaaagga ttagatgtac aagctacttt
     7381 agaaccaata acatatagca ctgtagatgt tgcaaagacg gaagaagtgg caggatactt
     7441 tttaggtcag atacttccat ttatattaat aataggagtt ttactaggtt caatatatcc
     7501 agcaatagat gttatggctg gagaaaagga aagaggtaca ttagaaacat tatttacact
     7561 cccaatatca aacttagaac tggttatggg aaaatatatg gcagtttctc ttagtgcaat
     7621 agtaacagca attttaaaca ttttatctat gggactgact atggcattta ttttagttag
     7681 tggaggtatt agtagccaat ttggctttgg aaactttaat tatggagact tagtcttacc
     7741 aattataact actttaattt gcatatgtct tttttcaatg gtagtttcag caataagtat
     7801 gtgtgtatgt tcattagcaa aaagttttaa agatgctcaa aactacataa cacctcttat
     7861 gttaattatt atgataccat catatgtttc tatgatacca aatatagtat tggatagagt
     7921 gacagctgta atacctgtag taaatatatc tttacttata aaaagtgtat tatcttttaa
     7981 aagcgattta aatttaatag ccttagtttt agcatcaaat gtagcctttg ttatattggc
     8041 aatcatacta ctgtctaaga tgttcaattc tgaggaaata ctttttggta atagtaagag
     8101 tttttctttc ttagagaaaa gaagtaatat aaaaaaaggg tctataccaa ctgtaagtga
     8161 tggtgttatt ttatatgcag taggattagt tctattgatt tatgttggtt ctttagttca
     8221 aattaaattt ggtatggcag gtattataat gacacaattc atgataattt ctttaccatt
     8281 attatttgca tgctatatta aagctgattt taaagaggta tttagcttga cagttcctaa
     8341 attaaagcat gtgtttggga gcattgttct ttggattggt ggatacatac ttattatgat
     8401 tataacacag ctaattttat tcttatttcc tcaaaatatg gaagttgctg aaagtttaaa
     8461 taaggctctt tttatcaaag atagtgtttt actgaattta ctagcaatag ctttattacc
     8521 agctatatgt gaagaactgt tttttagggg atttatattt tcttcattta gtaaatcaaa
     8581 agataaaaat aagtctatca aactcgctat aatatgtagt ggggtactat ttggtattat
     8641 gcatatggat tttattagaa taattccaac atcaatatta ggtattatat ttgcatacag
     8701 tgtgtataaa tctggttcaa tatttgtatc aatgttattg cattttttaa ataatagtgt
     8761 agcagttgtg ttaaatcatt atacaactgg aaatataacc aacttatata aatttgttga
     8821 agttgatttt tcgaacttaa atgtacttaa atttgcctta attcttctaa taagtcttat
     8881 attaattatg ttaggattaa gagttttaga tagaaagtct ttaagaaatt aagtataaag
     8941 ttattatata gattaaaata ttaataagca ctaaagtata tggatatatc ttgtatataa
     9001 aaatacttta gtgctatttt atatatttta attatatgaa ttatttatcg tttcatttat
     9061 gtaatgaaaa tgttatataa atgaaacaat aaataatttt tattttagaa atttatattt
     9121 aaataatcaa gttgaaagtg agatactaaa aaaattaatt taggaattat ttgatttatt
     9181 catttataaa aattaattac tgatttgtcg aaaccattgt aataagaaaa aaatgaaaaa
     9241 ataaagtgtg aatatgttgc aaatactgta ttttaataaa aagtag
//
LOCUS       DAEPOG010000147         9274 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEPOG010000147 DAEPOG010000000
VERSION     DAEPOG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.147"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            130..681
                     /gene="raiA"
                     /locus_tag="KR432_002898"
     CDS             130..681
                     /gene="raiA"
                     /locus_tag="KR432_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0641414.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            813..3488
                     /gene="secA"
                     /locus_tag="KR432_002899"
     CDS             813..3488
                     /gene="secA"
                     /locus_tag="KR432_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0641415.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            3503..4610
                     /gene="prfB"
                     /locus_tag="KR432_002900"
     CDS             join(3503..3577,3579..4610)
                     /gene="prfB"
                     /locus_tag="KR432_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH0641416.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            4754..6895
                     /locus_tag="KR432_002901"
     CDS             4754..6895
                     /locus_tag="KR432_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH0641417.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            7043..8209
                     /locus_tag="KR432_002902"
     CDS             7043..8209
                     /locus_tag="KR432_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641418.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      8269..8386
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8511..9116
                     /locus_tag="KR432_002903"
     CDS             8511..9116
                     /locus_tag="KR432_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0641419.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT         3854 a         1005 c         1790 g         2625 t
ORIGIN      
        1 taattctatc aattgaatag aaaaaatcat aaaagtaaat aataaaaaga ggattatgag
       61 agttcgtgta gaatatatta ttaaagtata aattttctta tactgacata attgaaggga
      121 gatgtattta tgaacataat tatatctgga aaacaaatcg aattaactga tgcaataaaa
      181 aataaaatcg aaactaaact aagtaagtta gataattaca ttcatccaga cactgatgta
      241 aaagttacag taagtgcgag aaaagcaaga caaaaaatag aagtaacaat agctactgta
      301 aatggtccta taattagagc agaagatatg caggaaaatt tatacacagc tatagatgtt
      361 gtatatgata aattaagtaa acaattaaaa aaatataaaa agagattaca agataaacat
      421 aaagacaaca agagtataag attccaagat tcagagataa atctagctga tgaacaagag
      481 gattatgggg atgaaaatca agaattagta attcaaagac gtaagaaatt tagtgttaag
      541 ccaatgagtg aagaagaagc tgtattacag atggaattaa taggacatga tttttatatg
      601 ttcaagaaca tagattcaga tgaaatagca gtagtttata agcgtaataa tggtggatat
      661 ggtataatag aacatgaata aagcaattta gatttaatca tattagctat cgataaataa
      721 attatcgata gctttttaaa ttaatattaa aagtatagtt tttatggtaa aatgaataat
      781 agtacaataa agaacgagga gagatgtaaa atatgagctt tatggataat ttattcaaca
      841 tggcagataa aaaagaatta aaaaagttca ataaaactgt tgatataata gattcattag
      901 aacctaaatt tgaatctatg gcagactcgg aacttaaaaa tatgacaaat atatttaaag
      961 aaagattagc aaatggtgaa agtatagatg atatacttcc agaagctttt gcagtagtta
     1021 gagaagtttc taagagagtg ttaggactta gacactacag agttcaaatg attggaggta
     1081 tagtactaca tcaaggaaga atagcagaaa tgaaaacagg tgaaggtaaa actttggttg
     1141 caactgctcc tgtttatttg aatgcgctta ctggaaaagg tgtacatgtt gtaacagtaa
     1201 atgattacct tgcaaaacgt gatagagacc aaatggcaaa aatctatgag tttttaggaa
     1261 tgagtgtagg cgttattata cacggtcaaa atccaaaagt tagaaaagaa caatatgatt
     1321 gtgatataac ttatggtaca aataatgaat atggatttga ctatctaaag gacaatatgg
     1381 taatacacaa agagcaaaga gttcaaagag gattaaatta tgctatagtg gatgaggttg
     1441 actctatact aatagatgag gctagaacac cacttatcat atctggacct ggagacaaat
     1501 caactcactt atattcagat gcaaacacat ttgttttaac cttaaaacca gatgattatg
     1561 aattagaaga aaaagataag gctgtatctc taacagcatc gggtatacaa aaagcagaag
     1621 tgtattttaa tgttgataat ataacagata tatctcatac agaattatac catcatataa
     1681 atcaagcttt aagagcacat gtaataatga agaaagatgt tgattatgtt gctaaagatg
     1741 gagaaatagt aatagtagat gagtttacag gtagacttat gtttggtaga agatattcag
     1801 aaggtttaca tcaagcaata gaggctaaag aaggtcttaa aatacaaaga gaatctaaaa
     1861 cactagctac tgtaacattc caaaactact ttagaatgta taaaaaactt tctggtatga
     1921 ctggtacagc aaaaacagaa gaagaagagt ttaaagcaat ttataaaatg gacgttttcc
     1981 aagtacctac caataagctt atggtaagag aagatttacc agattctgta tacaaaagtg
     2041 aaataggtaa atttaatgct gtagcacaag aaataattga aagacataaa gttaatcagc
     2101 caatacttgt aggtacagta tcgatagaaa aatcagaatt attatcacaa atattaaaga
     2161 aaaaaggcat taaacatgaa gtattaaatg ctaagcatca tgataaagag gcagagataa
     2221 tagcacaggc tggtagatta ggcgctgtta caatagctac aaatatggct ggccgtggta
     2281 cagatatagt tttaggtgga aacccagatt tcttaactaa gagagaaatg agaagaaatg
     2341 gatttaaaga ggaaatagta aatagggtag atactccaat agaaggtata cctgtaaaag
     2401 gtaatgaaat tctatttgaa gctagagaag agtatgaaaa actatttgaa aagtttaagc
     2461 aacaaacaca agaagaacaa aaacaagttg tagaagcagg tggacttgct ataataggta
     2521 cagaaagaca cgaatctcga agaatagata accagttgag aggtcgtgct ggtcgtcaag
     2581 gtgacccagg gagttcaaga ttctatatag gtcttgatga tgaccttatg agattgtttg
     2641 gtagtgatag aatatctggt attgtagata aaataggtct tgaagaagac atgccgatag
     2701 aacatagaat attatctaag tctattgaag gagcacaaaa gaaagtcgaa ggtaaaaact
     2761 ttggaataag aaagcatgta cttcaatatg atgatgttat gaataaacaa agagaaataa
     2821 tatatgcaga gagaaaacgt gtattagaag gtgaagattt acaagaacaa atacaatcaa
     2881 tgactcattc tataatagaa gaagcagtta ctctttatac acaagataaa ggatttgatg
     2941 aagaaggatt caaagaacat atgtataatc tgttcttgcc aaaaggtagc atagaaatac
     3001 cagaaataga aaaacttaat ccagttgaaa taacagaaaa agtttatgaa atagctatga
     3061 agatatatac ttcaaaagaa gaacaagttg gctatgagag aatgagagaa gttgagagag
     3121 taatcttact tcaagctgtt gataaccatt ggatagacca tatagatgct atggaccaat
     3181 tacgccaagg tattggtctt cgtgctgtag gtcagcaaga cccagttatt gcttataaaa
     3241 tggaaggttt tgatatgttt gatgaaatga ataaacatat caaagaagac actgtaagat
     3301 atttatttaa tataacaata gaaactccag tggaaagaaa agcagttgta gatgttgaaa
     3361 atctatcttc tccatcagat ggaacacttc caacaagtaa aactgttaaa aaagatgaaa
     3421 aggttggaag aaatgactta tgcccttgtg gaagtggtaa aaaatataaa aactgttgtg
     3481 gaagataatc tcggaggatt aaatgttaaa gttacaagag tatagacata gtatggagca
     3541 gatggcttca aatttagaag aattgagggt ttctctttga catagcttca ttgatgaaaa
     3601 atatacaaat taatgaagaa aagatgaatc aacaagactt ttggaatgat aatgaagtcg
     3661 ctcaaagagt acttcaagaa aataaatctt taaaagagac attagaagaa tatgaaagct
     3721 taaaaagctt attagaagat atagaagtat taattgaaat aggtttagaa gaagatgatg
     3781 attctgtaga aagagatata gaaaagtcca tagaatcaat ggaagaaaaa ttatctgaaa
     3841 tgaaaataaa gactcttcta aatggagagt atgataaaaa taatgctata ttatctataa
     3901 atgctggtac tggtggctta gatgctcaag attgggctca aatgcttttg agaatgtata
     3961 taagatggtc agaaagtaag ggttataaag ttaagctttt agatataata tcagaccctg
     4021 aagcagggat aaagacagct acaattcttg tagaaggaac aaatgcttat gggtatttaa
     4081 agagtgaaaa aggagttcat agattagtga gaatatctcc atttgaccca tcagggaaaa
     4141 gacatacttc atttgcatct atagatgtaa caccagagtt agatgaaaat atagaagtag
     4201 aaattaatcc gtcagattta aagatagaca catatagagc ttctggagct ggagggcaac
     4261 atgtaaatac tactgattca gcagttagaa taacacatat ccctactggt gtagtagttc
     4321 agtgccaaaa tgaaaggtct cagcatctaa ataaggacag agctatgaga cttttaatgg
     4381 cgaaattgat agaattaaaa gagttagagc aaaaggaaaa aatagaagat atacaaggca
     4441 aatactcaca aataacttgg ggtagtcaaa ttaggtcata tgtttttcaa ccatataaat
     4501 tggttaagga ccatagaaca aatgctgagt ttggaaatgt agatagtgta atgaatggaa
     4561 atatagactt gtttattaat gaatacttaa aaatgaataa aatagtttaa taaatatgca
     4621 ctgtaattaa gatatatgta taagaaggtt agtgcttaat atgcacttac agcacttagg
     4681 ctgaggacga taaccctcag ccttcgttct gttttgttat acttatgaaa aactaacatt
     4741 aggagtgaat aaattggata taaatcaaat cttaaaaaaa gagtttaatc ttagagatga
     4801 acaaataaat aatacattaa aacttattga tgaaggaaat acaataccat ttatagctag
     4861 atatagaaaa gaaatgactg gcgaaatgag tgatgttaca ctaagagaat tttatgaaaa
     4921 attaatgtat ttgagaaatc ttcaaagtag aaaagatgat gtagttagac taatagatga
     4981 acaagggaaa ttaacagatg aaataacaca aaatatagaa aaagcaaaaa cattacaaga
     5041 agtagaagat atatatgctc catataaaca aaagaaaaga actagagcaa caatagcaaa
     5101 agaaaaagga ttagaaaatt tagcattgtc aatactagag aataatttag ataatataga
     5161 aatagaggca aagaattatc ttgatgaaga gaaagaagtt ctttctattg aagatgctct
     5221 aaaaggtgct agagatataa ttgcagaact agtctcagat gatgctaaga ttagaaagta
     5281 tataagagaa ttagctttaa gagaaggtat gatagttagc aaaagtgcta ctgatgagaa
     5341 atcggtttat gatatgtatt atgattataa cgaagcagta aaaagtatgg ctccacatag
     5401 agttttggca ataaacagag gagaaaaaga aagcttccta aaggttaagt tagaaataaa
     5461 taatgataaa gttttaaatt acataataaa cgaatatgta aatgataaaa atttcaaaaa
     5521 taaagaagaa atcgtaagtt caatagaaga ttcttataaa agattaattt tcccatcaat
     5581 agaaagagaa ataagaaatc atttaacaga aatagctcaa gaaagagcta taagtgtatt
     5641 tggaaagaat gttaaaagtt tattgcttca accaccagtt aaagacaaag tagttatggg
     5701 atttgaccct gcatttagaa ctggatgtaa aatagctgta gtagataaaa atggtaaact
     5761 actagattat acaacagtat atccaacaga ccctcaaaat gatgtagagg gagcaaagaa
     5821 agttttaaaa ggattgattg aaaaatacga tatagatata atttcaattg gaaatggtac
     5881 tgcatctaga gaatcagaaa cttttgtatc agaaatgata aaagaaatag atagcgaagt
     5941 gcagtatgtg atagttagtg aagcaggagc atctgtttat tcagcatctg aacttgcaaa
     6001 tgaggaacat ccagatataa atgtatcaat aagaggagct atatctatag ctagaagatt
     6061 acaagaccca ttagcagagc ttgtaaaaat agaccctaaa agtattggtg ttggtcaata
     6121 tcagcatgat ttaaacaaaa agagattaga agaagtgcta gatggagttg ttgaagattc
     6181 tgtaaatagt gttggagtag atttaaatac cgcatcatac tccctactag aacatgttgc
     6241 aggaataagt aaagctatag caaaaaatat aatagcatat agagaagaaa atggagattt
     6301 tacatcaaga gcacaattga aaaaagtaaa aagactagga cctcaagcat ttactcaatg
     6361 tgctggattt atgagaatct tagagggtaa aaatccacta gataatacag gtgtgcatcc
     6421 tgaaagttat gatatatgta aaaaaatgat tgagataatt ggatattctt tagatgatgt
     6481 aaagaataag aatataggtg aaatagatga aaaaataaaa gaaattggct tgagggaatt
     6541 aagtgaaaaa ctggaagtag gacaagttac gttaaaagat atcatagctg aaatcaaaaa
     6601 accaggaaga gacccaagag aagaaggtat aaaacctata cttagaactg atgtcttaaa
     6661 aattgaagat atacaagagg gtatgacttt aaaaggtaca ataagaaatg ttgtagactt
     6721 tggtgcattt gtagatattg gaataaaaaa tgacggatta gttcataaat cagaaatgag
     6781 taatagtttt gtgaaagacc ctatgagtat tgttacagta ggtgatatag tagatgtaaa
     6841 agtcattgga attgacttaa acaaaaaaag agttgcctta tcaatgaaaa aatagataag
     6901 tttatgtatg gttatagtac ttaaattcta tatttgtaaa tgagattact cgagtgtata
     6961 aatgttaaaa atttgtaaaa gtatatagta agaaaatata ttaaaataag aacgatagtc
     7021 ttgaaaatca ggaggaaaat gaatgatttt tataaaaaat ggtaaaataa ataccataac
     7081 aaatgggata attcatggag atatacttat agatgaagga aaaataatag aaattggaga
     7141 agatttgata gctcctttag atgtagaggt aatagatgct agtaataaac ttgtgtttcc
     7201 tggatttata gatgctcata ctcacttagg tttatgggaa gatggtatag ggtttgaagg
     7261 agcagatgga aatgaagaga ctgacccaat aacaccacaa ttaaatccta tagatggaat
     7321 taatccaatg gatagaactt ttaaagaagc ttttgaaggt ggaataactt ctgtttgtac
     7381 aacccctgga agtgcaaatg ttatgggtgg tcaatgtata gctataaaga catgtggaaa
     7441 aagaattgat aaaatggtaa taaaaaatcc agttgcatct aaaattgctt ttggagaaaa
     7501 tccaaagagc tgttatggtc aggatgataa atctcctcaa actagaatgg ctatagctgc
     7561 tttacttaga gaaaacctaa aaaaagcaga agaatattta gaggatattg atatgtatga
     7621 gagtcatgat gatgaagact gtgaaaaacc tgaatatgat ataaaaatgg aaagtttaat
     7681 accagtttta agaagagaaa ttccttttaa ggcacatgct catagagcag atgatatgtt
     7741 tacagcaata agaattgcaa aagaatttaa tttaaagctt acattagacc attgtacaga
     7801 aggtcatctt attgtagatg aattagtgga agaagggttt cctgtaatag tagggccatc
     7861 tttgtcagaa aggtctaaat ttgagcttag aaatttgaca tttaatacag caggaatact
     7921 ttcaaatgca ggtcttgatg tatgtataat gacagaccat ccagtaatac cagttcaata
     7981 tcttccaata tgtgcaggta ttgcagtaaa acatggtatg aaagaagaaa aagcaataga
     8041 atcaattacg ataaatccag ctaagacttt aggaatagaa gatagagtag gctctataga
     8101 agttggtaaa gatgcagacc tagtaatttg ggataattct ccactagaga ttcaaagtaa
     8161 tgtactttac actataataa atggcaaagt agtgtatgag aaaaaataac ttgacaatta
     8221 ttaaaaaatt aactaatata agagtgtaag gaaaattaaa aaatcaaaga agatcttcgg
     8281 ggcagggtga aattccctac cggcggtata gcccgcgaac tacttaggtg gttgatttgg
     8341 tgagattcca aagccgacag taaagtctgg atgagagaag atatttatgg aagataaata
     8401 agtttatttt agtttgttta aactatatcc ataaaaactc gaagataggg tcttcgagtt
     8461 ttttgttttt cctaaaaaat ataaacatat tgattagggg gaaaataatt atgcaaaaca
     8521 cagcaaaaag aggcaatatg atttcaacaa gaactttagt agtaatgtca attttatctg
     8581 caatatctta tttattaatg tttattcaag caccaatacc aggtatattt ccagatttct
     8641 taaaaattga cttatcagat attccagcaa tattcggagg aatgtctcta ggaccattag
     8701 ttggatttgg aatagttgta gtaaaaaatg tgttacaagc aataacagca acaacaactg
     8761 gaggaatagg agaatttgca aatatattaa ttggtggttc ttatgtaatg atactttgtt
     8821 attcatataa gagaagtgga gatttaaaag gtgtactgat tggttttgta ttaggaacaa
     8881 tatctatgac tataattggt tcattagtaa attattttgt aatgttgcca ctttatggtc
     8941 aacttatggg actagatgct ataataggtt taggttcagc tataaatcct caagttcatg
     9001 atttattcac ttttgtaatt tggatgatag ctccatttaa tgtgctgaag gctgttataa
     9061 tatctttagt aacattgcca ctttataaaa aaatgggaaa tataataaag aaataattag
     9121 acaagaaaat ataaaattaa atgtattgaa tagccataat ttatttataa cttcagtcct
     9181 atattgtgat tgatatatta aatatattat ggctttatta atttatcaaa aaatgtatat
     9241 aaaataagaa atatttagac aaatttaaat ttta
//
LOCUS       DAEPOG010000148         9209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEPOG010000148 DAEPOG010000000
VERSION     DAEPOG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.148"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            715..945
                     /locus_tag="KR432_002904"
     CDS             715..945
                     /locus_tag="KR432_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBH0641420.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            958..1107
                     /locus_tag="KR432_002905"
     CDS             958..1107
                     /locus_tag="KR432_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBH0641421.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            1364..1801
                     /locus_tag="KR432_002906"
     CDS             1364..1801
                     /locus_tag="KR432_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBH0641422.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            2058..2951
                     /locus_tag="KR432_002907"
     CDS             2058..2951
                     /locus_tag="KR432_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0641423.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            3129..3953
                     /locus_tag="KR432_002908"
     CDS             3129..3953
                     /locus_tag="KR432_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641424.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            4052..4660
                     /locus_tag="KR432_002909"
     CDS             4052..4660
                     /locus_tag="KR432_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641425.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            4764..4940
                     /locus_tag="KR432_002910"
     CDS             4764..4940
                     /locus_tag="KR432_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641426.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            6236..6988
                     /locus_tag="KR432_002911"
     CDS             6236..6988
                     /locus_tag="KR432_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641427.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6978..9089
                     /locus_tag="KR432_002912"
     CDS             6978..9089
                     /locus_tag="KR432_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641428.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3538 a         1188 c         1178 g         3305 t
ORIGIN      
        1 atagtacatc attatctaaa tagtatcttt taaacgtact ctgctttaca aattaaaaaa
       61 attaactctt tatataaaag attaagttat ataacgcact taatacgcta tagatttggc
      121 agattataac ttcaattttg gttataactt taatatatta atgctttgaa aaattaatat
      181 attaaacgta tttcaactta aacatgttag ctattttaaa tgctacccta ctttaatcta
      241 attaattgaa ataaattaaa tattttctat atcagcaata actagagtca acaattaaaa
      301 tactaatatt gacaattgaa acactgatac taacaaacaa aacactaata ttgacaattg
      361 aaacactgat actaacaaac aaaacactaa tattgacaat tgaaatacta atactaacaa
      421 gcaaaacact aatattgaca attgaaatac taatactaac aagcaaaaca ctaatattga
      481 caattgaaat attaatacta acaagcaaaa cactaatatt gacaattgaa atattaatac
      541 taacaagcaa aacactaata ttgacaattg aaatactaat actaacaagt aaaacattaa
      601 tattgacaat tgaaatatta atactaacaa gcaaaacact aatattgaca attaaaatac
      661 caatattaac aagcaaacaa ttaaaatata aattattata tcggaggtaa ttatatggaa
      721 attaagaaaa aattagtcat gagctttaaa aatgtaaagg ataaacaagt ttctttttct
      781 atagaaaatc caaaggatga tttaactgaa gaaaatataa aatctgtcat ggatttgata
      841 gtatctaaaa atattttttc ggttggagga tttgacctag cttctgttgt tgaagccaag
      901 gttgtggaaa caaataccac tccttatgac ttagttatag aatagggggc taattttatg
      961 gatgatagtt tacaactgtt aataaccaat gttggttttc ctatagctct atccatgtac
     1021 ttgttggtta gaattgaggg taaattaaat actttatcaa acagtattga tacattatcc
     1081 aaaaatattt tgatatttaa gaagtaattt gacaatatat gtaagtcata aaataggtct
     1141 atttgtaaaa taattagaca tattctatgc taaatactag gatatttgga taatttatcc
     1201 aaatatccat agtatttttt tgaattttac cacctcaaat ttgaatttca ctattttatt
     1261 tttgaatttc actatgctta atttgaaatt tagaacgcat ctagttggac tcaataagat
     1321 ataatgctat tctaatgaca gtaataaaat gaggtgatga ttaatgaaaa tacatgtcga
     1381 acaaggtatt aattgtgatg atattgaaat aaccataaaa tgttctgctc tagataataa
     1441 cattgaaaaa ataatttcat tattgagtac accattactt gatataaaag gtaaaaaatc
     1501 aggagagatt tataaactca atgtctgtga tattttatac tttgaaacag ttggaaataa
     1561 aacatttgct tattatgaag atgatgtatt tgaaattgat ttaaaaatat ataaattatc
     1621 aaaattatta gctaatacat ccttctcacg aatatctaaa tccatgattt taaatatgga
     1681 ctacataaaa agtataaaag taatatcaca taatcgcatg caagctactt taaaaaacaa
     1741 cgaaaaagtt attataaatc gtcgttatat aaaaaatatt aaagaaaagc taaacattta
     1801 ataaaaatct aaagtaattt attaatatca ctaattgcaa atttattgac ctatttaata
     1861 attaataata atataatatg ttcaatatta aatactaatt aattatattt agtgtaaaaa
     1921 taattaatca aaatataaaa aataggatat tattataggc aataactaac atcaaacgat
     1981 aacttaatac atataaaaat aaataaacaa ttaataaaaa ttaaaacttc attataaaac
     2041 ggaaggatag tgaaaaaatg caactatata acaccccctt taatacaaac aaacacttaa
     2101 tctatttctt tacatctacg ctagtattga actggatatt cagtcttaca cctgcgttgc
     2161 tcggaattac aaatacaata cttggagaaa tcatttttta tcttgcatat acatcaccag
     2221 ctattgtagg tctatttttt atttctacaa tctatccaca aagtgcaaaa caagatttta
     2281 tttatagata cctaagcttt aaagaaatta gaataaaatg gatactatta acaatattat
     2341 tttttgcttt aacttttatt acttcgttaa taatcggaaa ttactttaat gtaaaaactt
     2401 ctgatataga ttggatacac atagcagtgt ttagaccttc taaaatagct tatatgctat
     2461 tagcaagttt tatattgagt atacttagtc aagaagctgg atggagagga tacgccattg
     2521 acaaattatt agttagattt ggatttatag gctcttcaat tattttaggg cttgtttgtg
     2581 gaatctggta tctagggtcg tattttacac ctaatcaaat accatataat ttggcacaat
     2641 actccctatt tgatgctttt ttatttatac caaacattgt acttttaaac tttataatta
     2701 attttgtata tatcaataca agtagaagca ttttatcagc tgggttagtt catatgatgt
     2761 actatttttt caacattcaa ttacttctac actaccctat taaattatgt gtaatagctc
     2821 aatatgtaca aataatattt ggtttaatat ttcttgtata tatgatttct tctaataaat
     2881 ttaaacagaa ggtaaattct gaaattgaac agataaagtc ggatgatttt gaatttgagt
     2941 tagactggta attttaatat gaattataaa ttaagataat ctatgatttg atacgattac
     3001 tacttaaata taatttactc tctataattt attgcaacat ctggaaaata attgtatttc
     3061 cacaatcgct tgaagtatat aaaaacaaaa tatatactaa ttataagtga aaaggaggtc
     3121 atattttcat gtttaaaata ggagaggttt caaaactaac acaaatttct attcgtatgt
     3181 tgcgttatta tgatgaactt ggtattttaa agcctgcaga aatagacaaa tatacaggtc
     3241 atagattata ctcagttgaa cagatttcaa tattacaaag aattgtatta ttgagagatt
     3301 caaaatttag cgttgcagaa atagcaaata tcgtacataa ttggaatgat gaatttgtaa
     3361 ttaaagaatt gaatagaaaa aagaatgaaa tacaaaagga aataaagcac gaacaacaaa
     3421 gaataaataa aattgataaa tttatagaag ctataaactg tgataaagat gaaattcatt
     3481 acaatgttgt ctttaaaaag attccaagtt ataaaatcat atcacttaga gaggtagtcc
     3541 cagactacca atctgaaggt atactctggg aaaagttatc taaatttatt aaagaagaac
     3601 atatagaagt atcacgtcaa cccaacaata atatagcttt ttaccatgat gaagaagtca
     3661 aagataatgg tgtagatatt gaagttggaa tggttgtaaa gaagattgga aagaacaaat
     3721 ctggatttat atatcgtgag acagaagaaa ttgatatgat ggcatgtact atggtttatg
     3781 gtccatatga aaatatagct ggtgcatatg aatcgttctg ttattggtta gataaaaata
     3841 gtgattatca aatatcaggt ataaatagac aaatcggtca taagggtgag cataatgaaa
     3901 taaatcctga aaattatctt acagaaattc aaatccccct tataaaagtc taatcgatta
     3961 gtacttaaat acttaagtac taatcaccca ttgacatccc aaaattttca tgatagtatc
     4021 taaacgtgta ttaaacatgt ttaatttttt tatggagaaa cttttaaggg gaaaaattat
     4081 gaaagcagat tcaacaaagg aacaattaat taatgccaca atccagttac tatcttcaca
     4141 tagagatgtt tcaagcatta cggctagaga aattgtagct aaagcaaatg tcaatttagc
     4201 tatgattaat tattatttta aatctaaaga ggagttaatt aatatttcta ttaaaaaatt
     4261 tttaaagcgt catatggatg aatataaggc tacacatgat actaaaaatt tagcacctaa
     4321 acagcaatta agggaaatac taattgcatt ttttgatttt ataggtgaac atagccaatt
     4381 tataaagatt tctgtaccct atatattgat acaagaagaa attgtttatc catttgagat
     4441 actaccttta attaaggctc actatgaaaa tcagaataac aaaaaaactg aagctgaata
     4501 cagaactatt gcgtatcaaa taacttcatt tattcagttg ctttttcttc gttctaatgc
     4561 ttttttcaag tatagtggta ttgatattct taatgttgaa cagagaaatg gtttaataga
     4621 ttttcaatta gatttattat tggtagatat tacaaaataa tactatatta attttttatg
     4681 tacatttttt tattttatgg taacgtttga aaatattctc attgactaaa aatacttgca
     4741 ttaaataagg gggaggaatt ttaatgtttt ttacatttat aggagtaata tcagcaattt
     4801 tattgttgtg tacaattata tgtggtttat ggataaaact taataatcgt aataatgact
     4861 ctaattctca ctttactttt tcaatacttt caattgcttt ttcactgtta attgtggtgg
     4921 cctttttagt aagaaattaa tgtctttgtt atgctggggt ttattattat agatttttat
     4981 tattgcatga tttttactat attatagtat ttttattata ttacagggtc ttatttatta
     5041 tagtgttttg ttattacagc attttattat attacagagt cctatttatt ataggatttt
     5101 tattatattg taaaatctta tttattatag gattttgtta tattacagac ttttattata
     5161 ttacaatctc ttatttattg cagtgttttg ttattacagt atttctatta tattgcaaac
     5221 tcttatttat tatggtgttt tgttattgca acatttctat tatattataa agtcttattt
     5281 attacagttc tttgttatac tacagacttt tattattaca gcatttctat tacaatctct
     5341 tatttattac agttctttgc tatactacag acctttatta ttgcaacatt tctattacat
     5401 tgcaaagtct tatttattac agttctttat tatactacag acttttatta ttacaacatt
     5461 tctattatat tacaatctct tatttattac agttctttat tatactacag acttttatta
     5521 ttacagcatt tctattacat tgcaaagtct tatttattac agttctttat tatactacaa
     5581 acctttatta ttacaacatt ttattatttt aacgtcttta ctataatata gtaatatttt
     5641 aatatctcaa aatcatatca atcacttttc agcatagtct aaaatacttc cactttctaa
     5701 aattatctat catacctata ctaataatac tttttaacct ttttattata catttcaatt
     5761 gtttttaacg acctatttat ttccaaaact ccaatatatt ttctcaatta cattgtgtta
     5821 ataaccccta aatcctttca aattatttac aatacattcc attaaataat cttactgctc
     5881 tataattctt tttttctttt taaccctact atacattatt tacatcattt ttttcaatac
     5941 aacattcgtt attctataac ctttttactt ttactatata ccttttttct aactacacct
     6001 tcaatgttcc acatcatatt actttagcac aacaattaat acctaaaaat aaccctctaa
     6061 atcctccaat aatataatca atttaactat aataataacc tctcacacta tccaaacaat
     6121 atattttcaa acattacaaa aaaacatcca actattacaa tattgctcat atccctcctc
     6181 ccctttcaaa tgctatcatt gacttacaag ataaatgaaa gtgaggatga tatatatgct
     6241 taaagtaaac caattatgta aatcatataa aacaggcaat aaaacatatc ctgtattaaa
     6301 aaatatttct tttgaaatca acaaaggaga gtttgtcgca atcatgggtt catcaggaag
     6361 tggaaaaact actcttttaa attgtatttc atgttttatc ccaattgata atggggaaat
     6421 aattcttgga aataataata taagaaattt aaaagaaaag gaactttcag aagttagaaa
     6481 ccaacaacta ggatttgtat tccaagattt catgctattt gatggtctta gtgtctttga
     6541 aaatatatgt gttccacaaa ttatcaaagg tagtcaagtc ttacaaatgg aaaaccgtgc
     6601 aattaatcta tgcgagctat ttggtattga acatatcaga aaaaaatacc ctgccgaaat
     6661 atctggtgga gaaaaacagc gtacagcagt agcaagagca ctcatgaaca acccagattt
     6721 aatattagca gatgaaccaa ctggtaacct tgatagtaag tcttgtcgta cagtcattga
     6781 atcattttta aaagcaaagc aagaattaga tgctactata ctcatggtta cacacgatag
     6841 ctttgcagca agcttctgcg accgagttat tgtgcttaaa gatggaataa tttataaaga
     6901 agttgtaaaa tcaaattcaa gacataaatt cctagatgaa ttactagaag ttttaaaaga
     6961 aatgggaggc gctcaagatg acaattaata aattatacag caaacttcga gcctataatt
     7021 ggaagaacta tacaacttta ttcttttgta taatactttc tgtggtatta gtaacttcat
     7081 atgctctaat ttattttagc ccaacaatac aaaacatact tccaactggt ggagatagca
     7141 gaaaacaggc tacaatgata ttctcagttg ctatattagg atgcgctgtc tttactacat
     7201 acgcttcttc cctcttttta aaatttaaaa gcaaggaaat tggtattttc atggctctag
     7261 gaacagataa gaagaaatta aaaaaacttc tattttcaga gatattgtta atttcaatag
     7321 tatgctgttt tttaggtctt atattaagta ttcctgtatc ttttggtata tggaaaatat
     7381 ttcaattagt gattattgat accagagaaa tgacatatca ttttggaact caaggattca
     7441 tatatggatt tatatttagt gtatttgtga cattatgcat atttatactt tctgtaaaat
     7501 ttattaatcg tacaaatata attgatatat taaatgacca gagaatatct gaaccaatac
     7561 aggatgtaaa ggactggtat ggtatattag gtataatttt tattatacta ggtatttttc
     7621 taggatatat cgtaccttca ctaactataa acaaattaaa ttatagacta ccatctgtat
     7681 ggagctttac ttatctactc tctcttattg gaatatatat gattatgaca tacatagttg
     7741 tacatgctaa aagaggaaaa catccagaaa aatactataa aaacatcata tcaactagca
     7801 tgatgagatt tatgggaaga caaacagtaa aaaacatgtg tgttatttca tttttgattg
     7861 caggagcatt atttgctgta ttctatgttc ctaatatggt tgcaggtatc tatgatactc
     7921 ttaaaaataa ccctatagat tatactttta cctacaatca aagagataaa caggtatcaa
     7981 aaaaagatat tgaacaatta gcagataaac acaatataaa aattcaaaac tatatggaaa
     8041 ttccttctat agaattaatt gttgatggtg tagaatcaat atatcataaa gatggaacta
     8101 tcgaaagggt atatatgaaa aaaagctcct atgatatgtt ctttaattct aaagattttt
     8161 ccaagctaat tgacaaacca ataactataa aaaatggtga atatcttgct gttgtaaata
     8221 aaggaaatga aaatcaagat tataaatata ccaaatttat atcaagccca gtcacagaaa
     8281 aagaaatccc ctttagatat gctggaaaaa taatatttaa tggttctttt tttcaaaata
     8341 gaagtatgaa cgcctatgtc cttaatgatg aggattataa atcatttact aaagatttat
     8401 ccataaaaaa ttacaataat tctatactat ttaatgtaga taaagatacc tatgcttttg
     8461 ccaatgactt aaagaaagaa attataaaaa gaacatctaa ggaaatgggc atgttcagtg
     8521 gttatgatga atacgtaaaa aaacgttatg aatcaactgg aagaaaatat tttatggatg
     8581 aagaatatcc aagtggtgaa ggtcatttaa aactagaccc aaacaacaat cagttgtatc
     8641 ttaattggaa atactatcct aactttaaaa ttttacaaag ccaagatatg ataaaaaaca
     8701 tggcagtctt cttaatgtta ttcatatatg tggcaatcat atgttttaca gcagtaggaa
     8761 tcattgcata tacaaggggt gttactatag ctataaatca caagcaggtc tttatggatt
     8821 taaatcgatt aggagcaaac aatgattata taaagttctg tattagaaat caattaaaga
     8881 aagtattttc ataccctctt attgcaggaa gttttttaat ttatctgttt aattttatga
     8941 ttatgttaaa taatgatggt agaatcgtca atagtgaggt caatgctctt ttgataaact
     9001 taggttttat agggatttgt ggagtttata tgtaccttat atatctatta acttataaga
     9061 aattcaaaaa aattattggt attttataat aaggaaggtt gttagatttt taaagtaata
     9121 aaatcattac tttttataat aagaaaagca ttagcatttt aaattaaaaa aggttattag
     9181 atttttatga tatgaaaagt cattgtatt
//
LOCUS       DAEPOG010000149         9042 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEPOG010000149 DAEPOG010000000
VERSION     DAEPOG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.149"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            170..616
                     /locus_tag="KR432_002913"
     CDS             170..616
                     /locus_tag="KR432_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641429.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            705..2690
                     /locus_tag="KR432_002914"
     CDS             705..2690
                     /locus_tag="KR432_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBH0641430.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            2914..3882
                     /gene="spoIIIAA"
                     /locus_tag="KR432_002915"
     CDS             2914..3882
                     /gene="spoIIIAA"
                     /locus_tag="KR432_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBH0641431.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            3872..4393
                     /locus_tag="KR432_002916"
     CDS             3872..4393
                     /locus_tag="KR432_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBH0641432.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            4410..4607
                     /gene="spoIIIAC"
                     /locus_tag="KR432_002917"
     CDS             4410..4607
                     /gene="spoIIIAC"
                     /locus_tag="KR432_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBH0641433.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            4623..5009
                     /gene="spoIIIAD"
                     /locus_tag="KR432_002918"
     CDS             4623..5009
                     /gene="spoIIIAD"
                     /locus_tag="KR432_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBH0641434.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            5094..6299
                     /gene="spoIIIAE"
                     /locus_tag="KR432_002919"
     CDS             5094..6299
                     /gene="spoIIIAE"
                     /locus_tag="KR432_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBH0641435.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            6359..6976
                     /locus_tag="KR432_002920"
     CDS             6359..6976
                     /locus_tag="KR432_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBH0641436.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            6988..7542
                     /locus_tag="KR432_002921"
     CDS             6988..7542
                     /locus_tag="KR432_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBH0641437.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            7569..8258
                     /locus_tag="KR432_002922"
     CDS             7569..8258
                     /locus_tag="KR432_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBH0641438.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            8368..8730
                     /locus_tag="KR432_002923"
     CDS             8368..8730
                     /locus_tag="KR432_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0641439.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            8815..>9042
                     /locus_tag="KR432_002924"
     CDS             8815..>9042
                     /locus_tag="KR432_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen utilization protein B"
                     /protein_id="HBH0641440.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVID"
BASE COUNT         3742 a          885 c         1647 g         2768 t
ORIGIN      
        1 aaatgaaaaa tattgtaaca acagaagaac aatagtatta tgtttataga taaaaaactt
       61 attaggaggt aaatttatgc caacaataac attaaaaaat ggtgtagacg tacttattag
      121 agaaggtgta agagaagatg cttaaagcat tatagatttt tataatgaag tgggtggaga
      181 aactcatttt ctgtcatttg gaaaggatga atataaaata tctttggagg aacaagaaaa
      241 tgctatagaa tcagcaaaag catctgataa ttctgttaaa ctgattgcat ttatagatga
      301 tgaaatagtt gggattgcaa cgattgattc taatcaaaaa gcaaaaggaa agcatgttgg
      361 agtattagga attgttgtaa aagaaaaata ttggggaatt ggattgggta aaaggcttat
      421 gttagacctt atagaatggt gtaaatcaaa tggaataacc aagaagataa ctcttgtcac
      481 taatgaagaa aattataatg ctataggatt atacaaaaaa gttggatttg aagtagaaag
      541 catactaaaa aaagaatgtt attataatgg tgtttatact gatttaatag gaatgtcact
      601 tttattggga atataattag tgtacatttt atctcgaaaa aagtatatta agtatggtat
      661 aattaaaaaa gaatataact taatataatt ttggaggacg aattatgaaa aatctgtgta
      721 aaatatcaga tgattattta aacagcaagc ttaagtattt aaagttatta tcaaaacaat
      781 atccaagtat atcaaaagca agtacagaaa taataaattt agaggctata ttaaatcttc
      841 caaagggaac agagcatttt ataacagatg tacatggtga atatgaacca tttgtacatg
      901 tattaaaaaa tggttctggt gtaataaaga gaaaaataga agaattgttt tcaaatacaa
      961 taagagacag cgaaaaaaaa atgttagcaa ctcttgtata ttatcctgaa caaaagttag
     1021 acctaataat taagcaagag gaaaatatag atgattttta tagaataaat atttatagac
     1081 ttatagaact ttgtaaatat gcttctagca aatatactag gtctaaagtt agaaaactgt
     1141 taccagaaaa ttttaaatac ataatagaag aacttttgca tgagcatgtt aaaagtgaac
     1201 ataaagaaga gtattataaa agtatagttg aaacaatagt tgacatagga atagctaagg
     1261 agtttataat tgctatttct acagttatac aaaaattggt tgtagataga cttcatgtaa
     1321 taggagatat ttatgataga ggtccaagac cagatataat tgtcgataag cttattgaac
     1381 atcattgtgt ggatattcaa tggggaaatc atgatatatt gtggatgggt gcagcctcag
     1441 gtgaaaaaac ttgtatagca aatgcactga ggatatcagc tagatatgcg aatttagaca
     1501 ttgtagaaga catatatgga ataaatctgt tgcctttagc cacttttgct atagaaatgt
     1561 ataaagatga cccttgtaaa gaatttattc caaaggttaa tgaccaaagt gtaacaacaa
     1621 cagaaaagtc tttaatggca aaaatgcaca aagcaataag tataattcaa tttaagcttg
     1681 aaggtgaggt tataagacga agacctgaat ttgaaatgga acatagactt ttgcttaata
     1741 tgataaacta tgatgaaggt acgattactt taaaaggtaa aacatataaa ttaaaagata
     1801 cttatttacc tacaatagac aaaaaagacc catataaatt aactatggaa gaaagaaatg
     1861 ttattgataa attagtatct tcatttagag gtagtgaaaa attacaaaaa catgtttcat
     1921 ttttgttttc taaaggaagt atatatttaa aggctaactc aaatttactg attcatggat
     1981 gtgtaccatt aaatgaagat ggaagcttta tgtctatgaa tataatggga aaagagtata
     2041 aaggtaaggc tcttatggat aagatggagt ctctagcaag agaaggattt ttctttaaag
     2101 ataaggcaga agaaaaacta tatggtatgg atattatgtg gtatttatgg actgggaaat
     2161 gttcatcatt atttggtaaa gatgatatga ctacatttga aagatacttt atagaagaaa
     2221 aagagactca taaagaaaat aagaatccat attttaaact tagagaaaat gagatggctt
     2281 gtaaaagatt atttgaagaa tttgatttag agcttgatga atcccatata ataaatggtc
     2341 atgttcctgt ggagagtaaa aatggagaga gcccaataaa agctaatggt aaaattctcg
     2401 ttatagatgg tggtttttct agggcttacc aaaaaactac tggtatagct ggatacactt
     2461 tgatatacaa ttctagaact ttgcaattag tatcgcatga accatttaat tctgctgaag
     2521 aagccattgc aaatgaaagt gatatcttat cgacaactgt tgtagtagag cataaggcaa
     2581 agagaaaaat ggtgagagat actgatgaag gagtaaaaat acaagaggaa atagaagatt
     2641 taaaactact tttaatggct tataagaaag ggctaataaa agaaatgtag atattataga
     2701 ggcacttcat aattgaagtg tcttcttgtt aatacttatt aaatatatta taaagattaa
     2761 taatagtagt ttatattata cttactaagt gaatccaaga taaatgggtt cacttttttt
     2821 atttataaac attattaaaa aaatatagat gttttggtta tattccttgt cctttataaa
     2881 tagaattata taaacaaagg aggaggacaa gagatgaata aactttctga tgaaataata
     2941 aactctcttt ctacaactat aagagaaaag gtagaaaaag tctcaaacaa taatctgaac
     3001 atagaagaaa ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat
     3061 tatttttaca atcaaaaaac tatgacactt gatttaaatc aacaaaactc atatgtagta
     3121 acaagagaag atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt
     3181 atggatgaca taaaaaaagg attcataact ttgagaggag gtcatagagt agggttagtt
     3241 ggaaaagcaa tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat
     3301 ataagggtat caagggaaat tattggatgc tcggataaga ttttaagtca tattataaag
     3361 ggaaaaaatc agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact
     3421 ttaataagag atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt
     3481 ttaaaagtag ctttggtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca
     3541 caaatggatg tgggaataag aactgatata atagaaactt gcccaaaaga tttgggtatt
     3601 acgatgcttt taaggtctat gtcaccaaat gttattgtga cagatgaaat tgggagtgaa
     3661 aaagaaataa aggcattata cacagcatta aatggaggta taggattaat aactactgtg
     3721 catggagatt caatagatga tatacaaaat agaaaagaat taaatagact tctggataaa
     3781 gagttattta aaaaggttat aattctttct gcaaaaagag gagcaggaac gattgaaaaa
     3841 atttatgatt tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagctct
     3901 cttggtagga agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca
     3961 taaacaatta aacgatttaa taagagtttt agaaattttg aggatggatt tatctttcgg
     4021 actttataca ttagaagaaa tctttaatag gataggtgga aataaagagt tttgtttttg
     4081 gaaatttttc tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat
     4141 aataatatca gaaaatattg atgtcttaag taaagaaact tatctaggca ataaagaaat
     4201 tgaggaactg aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag
     4261 aatgatagat ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat
     4321 aaacaaaaaa ggcgtattgt ataaaaaatt agtgacattc ataggtatag gtatatgtat
     4381 tatattgatt taatatgggg aggtttatta tggaaatatc attgatatta aaggttgcag
     4441 gtgtcggtat tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt
     4501 gggcaggtct tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa
     4561 taagtgactt gtttaatact gtaagaacaa tgtttcaact ttactaaaaa ggagggagta
     4621 atttggaaat aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa
     4681 ttaaaaaaga tagaccagag atagcaaatt tcatagcaat aataactgga gtaataatac
     4741 tattgtcagt tatgtttaag ctaaatttta tagtagatag tatacaagat ttggctaaca
     4801 aggctaacat accgactatg tatatatcat taataataaa attaattggg atagcatact
     4861 taatggaatt tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac
     4921 tagaatttgg aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg
     4981 tagaaatggt ggtgaatata attccataga tgtgtatctt ggaatgaata taagtatatt
     5041 agagaacttt cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa
     5101 aaagattttt accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg
     5161 ctattatgat atttgctaat gaagtaccaa gtagtgaaga taaagaatat ggtgaaacaa
     5221 aaaatagcat agataaatat atagatggtc aattagacaa gctagatata aatgagattc
     5281 aagactatat aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag
     5341 atttaattag tggagaaaaa aatatcttag atttattcaa taaagatgga ttgaagatat
     5401 tgatgtttga tgaatttaaa gcaagtttaa aggtagtagc agtaattttg gtactggctc
     5461 tgttatcatc tattttaaag agtctggaaa actctttttc atcaggtgca gtaagtcaaa
     5521 tagcaacata tataatattt atcacaatgg tatcacttac acttattggt tttaaagatg
     5581 tattacagat ttgttatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc
     5641 caatattaat aacattttta cttttaattg ggttcccaat aacgtcgact actttgaatc
     5701 ctatcttcat aggaggagta acatttataa atgtattttt taaaaatttt ctatttgtat
     5761 ctattactgt tgcatttgga attttaataa taaacaattt atcaaaaaat ataaggctaa
     5821 agagattttt ctcctttgta aaacaaataa attatgtttc tattggagct atgtttactg
     5881 tttacttagg tcttgtatcc atacaaggat tgtatgtaac cagttttgat aaattcagtg
     5941 taaagactgc taaatttgct ataggtaatt ttattcctgt tgtaggtgga tttgtatctg
     6001 actctgtgga tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag
     6061 ggcttatctt acttgttgga atttgtttac ttccagtaat aaaaatttta tcagtaatag
     6121 tagtgtacaa attagcagca ataattgtag agcctgttgg agaggatgga atatctaatt
     6181 ttttaaatga agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag
     6241 taatgttctt tgtaactgta gctatcttga cctcgataag tgtcgtaagt caagggtaat
     6301 ttatatatca gtgaaaaata attacattta atctaatgtt tttagagagg aggatgctat
     6361 gttagagggc ataaaggcat ggatagttag tgttttgata ggagcattta tagtaaatat
     6421 agtagatatg attttaccaa gttctaagat aaaaccttat gtcaacctag ttttaaactt
     6481 tatgtttgta tttattgtaa taactccagt agtaggattt ttttcaaaag atatgagttt
     6541 agaagataga atattaaaat ctatgggaaa ctacaacaag cagtatgtag atagtacaaa
     6601 tgctttggca aaagagactg gaaataatag tctatccaag ggatatgagg atgggttaaa
     6661 agaagtactt aaattaaaac ttgatgaata tggttatgat ttagaagata tagagcttaa
     6721 tggggcaaat ataaataata taaaaataaa agaaaagaat aatagtgcaa aaagtaataa
     6781 cagttccaat gaagaaaata aaaataatag tactaaaaga gaggaagaaa acataaactc
     6841 taatgacaaa gaaaattcta aacaagtctt caaaaaaggt acagagtatg ggttgaacct
     6901 aaatgaagaa aaattaaaaa acgacttaat aaaggtgctg gatgtttcta tagaagatat
     6961 acaaattgat aaataggagt tgggagaatg ttgaaaaact taaatgacaa agataagaga
     7021 aaggtatatt cactaataac tatagcagga atatgtgtgg tttcattagt attattatct
     7081 tgttttccta gtagcaaaag tgataaacaa gttggaaaaa cagagactaa taaaaatgct
     7141 gaaaagcaag tcactaaaaa acaagaaaaa gatgatttag agtcaaaatt aacagcaata
     7201 ctatctaaaa ttgatggtgc aggagacgtt gatgtaatgg ttacatttga atcaagtgaa
     7261 gaaatacaac ctgcttttaa ttcaaataat acaacagaaa caacagagga aaaagatgca
     7321 caaggtggag aaagaacagt tacgacttct agtgaaaata aaacaatgat aacttcaaat
     7381 tcaagtgacc ctgtagttat aaaaactaca gagccaaaga taaagggagt gatagtggta
     7441 gcaagtggag caagtgaccc aagtgttaaa gagacacttt atagtgcagt acaaacatca
     7501 ttacaagtag ctggtcatca agtagaaata tattctaaat aaatacatat aaccgggggg
     7561 ataaatgaat gaagtttaat tataagggaa gaggatttgt aataataact ttaactgcaa
     7621 tgttagtggt agtaggaaca gtgaattatc aactaagtaa gaaatcttta ttagaaacat
     7681 caaaagagtt taaggcatat gaagaagcac agttgcaaaa aaatactgat gatagtgata
     7741 gtagtaagac agaggattct agtaatgtag ataaacaagg tggtaaggaa agtgctgata
     7801 ttgatatagt cgatagcaaa gctagtaaag taaaagaaaa ggcaactgag acaagtaagg
     7861 aaataaaagc tcaattatca tcagagaaaa atatgaaaaa ggcatcttat attttagata
     7921 tgaaaatgaa tagagaaaaa caaagaaatg agttagttca agatttaaat gaaatgataa
     7981 ataatccatc tacaacagaa gaatctagaa aagaagcttc taatatgaaa ttaaatatag
     8041 ttaaaattca agaaaaagaa cttcaaatag aaaacctttt gagtacaaaa ggatatgaag
     8101 aagcgttagt ttatataagc gataacaaag ttaatgtagt tgtaaatgaa gctaaattag
     8161 aaaagaaaga tgcagcaaaa atatttgatt tagttgcaga acaagccaat gtgaagtatg
     8221 aaaatattaa acttacaaat aataatagta ataagtaaag tataaacctc agaattaact
     8281 gaggttattt tcttgcaaag tagacttgta tttggtatta ttatacttat aaaaatagtt
     8341 ccttaaaatc aggaggtaag ttttaatatg gaagataata aatttggaca agtaaaaata
     8401 tctaatgatg taatagctac aatagctggt ttagcagcat tagaagttga aggcatagaa
     8461 actacagcaa cattaacaga taagttgtta aaaaataatg gagtaaaaat acaaatcgaa
     8521 gaagaggatg ttaatttaga tgtaatggtt acgataaagt atggaatgtc aataccagat
     8581 acagctttta aagttcaaga aaatgtaaaa aatactgttg agacaatgac aggactgaag
     8641 gtttctcaag taaatataca cattcaagga atcagcttta agaaggataa agttgataaa
     8701 gaagaagcaa aagcaactaa gaaaaattaa tattagccct ctgaaaagtc agagggtttt
     8761 ttgctggtaa ttgattttat atggataatt aaacttaaag gagaattttg attgatgaaa
     8821 aaagataggg cacaaaagag tacaacaaga gaatatataa tgaaacttat ataccaaata
     8881 aatattaata aagaagattt tgaaacttta gaagataaag tagataattt tttaaaagat
     8941 aattcagaac atattataaa tagatataaa gaacttgcac ttcaatactc aaaaaataca
     9001 aatttaaagt tagaagatac tgaaattgaa gatgttatag ac
//
LOCUS       DAEPOG010000150         9005 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEPOG010000150 DAEPOG010000000
VERSION     DAEPOG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.150"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..360)
                     /locus_tag="KR432_002925"
     CDS             complement(<1..360)
                     /locus_tag="KR432_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0641441.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKS"
     gene            complement(487..1176)
                     /locus_tag="KR432_002926"
     CDS             complement(487..1176)
                     /locus_tag="KR432_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0641442.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1184..1747)
                     /locus_tag="KR432_002927"
     CDS             complement(1184..1747)
                     /locus_tag="KR432_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0641443.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1749..2717)
                     /locus_tag="KR432_002928"
     CDS             complement(1749..2717)
                     /locus_tag="KR432_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBH0641444.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(2818..2861)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3226..3855
                     /locus_tag="KR432_002929"
     CDS             3226..3855
                     /locus_tag="KR432_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641445.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            3830..4234
                     /locus_tag="KR432_002930"
     CDS             3830..4234
                     /locus_tag="KR432_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBH0641446.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4237..4710
                     /locus_tag="KR432_002931"
     CDS             4237..4710
                     /locus_tag="KR432_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0641447.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            5257..5922
                     /locus_tag="KR432_002932"
     CDS             5257..5922
                     /locus_tag="KR432_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBH0641448.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            5944..6876
                     /locus_tag="KR432_002933"
     CDS             5944..6876
                     /locus_tag="KR432_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBH0641449.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            6897..7538
                     /gene="pcp"
                     /locus_tag="KR432_002934"
     CDS             6897..7538
                     /gene="pcp"
                     /locus_tag="KR432_002934"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBH0641450.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(7657..7989)
                     /locus_tag="KR432_002935"
     CDS             complement(7657..7989)
                     /locus_tag="KR432_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641451.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            8255..8875
                     /locus_tag="KR432_002936"
     CDS             8255..8875
                     /locus_tag="KR432_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0641452.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
BASE COUNT         3327 a         1320 c         1177 g         3181 t
ORIGIN      
        1 agactttaaa ccagaacaaa acccatcgca aactgaccaa ttatcgattt taggtataaa
       61 tctttttaca tagtataaag tttcttcaaa attactattt atattgccaa ttaccatacc
      121 ttgtagccta acttcttcat agtatgtatc ctctgctact tctaaaaatt ccttccaatt
      181 cccttggctt atttcttttg ataactttct taaattagga actctaatac ctattatatt
      241 ttctgttcca ggtataagct ttttattaaa atctctatat tttatatctt ttatagacat
      301 catatattct aaaagttcat tataatccaa tttattccat tttcccctat caaacatcat
      361 ttaattgctc cttaattcct tttttcttat tgtaacataa aactctacaa aaaaagcttc
      421 taactattag aagctttttc aaaaacataa gaaacttttc aattttaaat ttaaactaaa
      481 ttaatgctaa tctcctacac caaagtctgt attattgtat aaaacttcta tatttggatt
      541 ttcatccaag aatttatact tactaacaaa ccatttcact aactcttcat ctctttttat
      601 actagaagaa ttatttataa aaacattcac tgtcgctttt tcaaaagtat ttaggataat
      661 ctctatatct ttatctatca tctcttttgt ttgtcctttt attccaatca taatacaagg
      721 tgaatcaaaa tattctttta catcttgtgg agtcttaaaa tttgcatttt tatttaaaat
      781 attatttcta aaatcattgt caaaggtttc aactccaatt ttaaatacca caggaatttc
      841 aaaatactct ctcatttcct taagcctatt tttgtaagac caatgacttt ctaaaaataa
      901 tttttttata ttcttctctt ttataataca ctttattttt tctaaagtat ccttaggtaa
      961 ttcaaaacag cttccagaat ttatgacttc aagaactcca tatttcccag ttacattttt
     1021 taaaaccctt agatttaatt tattcatctc ttcttctaaa ttagaattat catctatata
     1081 atcacaaaaa gaacactttc cccatataca aggaaatcct ttaagcagaa caatttctct
     1141 ttgattttta tttgttatct cactatatct atccattttc tatctatttt cctccagtca
     1201 atgatacctt taaagttttt ggaactgcat ttaatcctaa attcactaaa acaactgcta
     1261 acagcttatc tccataatca gtaaatactt gggttacaaa cacactaaaa acatctccca
     1321 gtcctaacac tttcaagatt tgcactatat aggatgaacc tgaagaggtc atacctccaa
     1381 ataaaaatgc tgaaattatt gaacttatta tagatgttgg aatagcaaat aaaagtactc
     1441 ctagtggagt ttttaaaccc cttaaaaaat tctttttaaa tgctattcca gcaagtaatc
     1501 cggttgatat ttgaactggt gcaaaataga gagaatacac atcaaacgta attccactta
     1561 ttaaacttcc aaacacacct gtaacaacag cgtatttagg tcctaaaagt gaagctatca
     1621 ttatagttcc tattgaatct aagtatacag gtaatcttaa tcccatagct ataaaagcgc
     1681 ctattatatt tatagaaata ccaaaagcta taagtgtaat agtttttaca catatttttc
     1741 ttttcacact atatcactcc ttccacagag tctataatgt cttcataacc tttaaaaagc
     1801 ctctttaaaa acatgtacat aaatttcttc tcatcaactt catttagaac aattgcatta
     1861 ggattttttt tatagaaatt gaaagaatcc actatagact gacccatagc tattccatct
     1921 tctacaactt ctacatatga ttcaaatcct ttacatatac ttctgtctat aaagtacgct
     1981 actgctagag ggtcatttat cacacagcca attattcctt cctgttccca atggaaatca
     2041 atataaaatc tagttatttc agttatatac tttgccatct tcttatcaag tctatttata
     2101 aactcaataa tattaggagt aagtacaatt tttctagtta catctaaacc taccatgtgg
     2161 atttttttag ataaattctt gtaaacataa tctgctccat gtgggtctac ccaataatta
     2221 aactctgcta ctggagagca atttccatga atcctaaatg ctcctcccat agatacaaac
     2281 tcatcgagat tttcaaatgc tttcttatct ttaataagtg ctttagctat atttgtaagt
     2341 ggtgcaagtg ctattattga tactttttca tgattatgta aagtatttat tataaaatcc
     2401 actgctccat tttttgcttt agctcctaca accttttgat aaaagttttc tccaatacca
     2461 tcttctccat gtgtatcttg agctgttaca agttttcttt ttagtggtgc ttcttctcct
     2521 atatatactg gaatatttag tgaagaacac atttgaagtg tttttagtgc attttctgca
     2581 cctatatttg ctggaacatt tccacaacat gtggtaattc caattacttc tagctctggt
     2641 gagtttaaag ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca atcaattatt
     2701 acttttctct tttccatttt ttatcctcct tgatttgtgg tcgaggagaa ctacacatct
     2761 tttttccact ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc actatataat
     2821 tccttagttt tttatacttg gagttctcga acaagtacag atgtaaaatc atatttttag
     2881 gctacttact atagactctt aaaaactata gactgttaaa aaatttttaa acatctgatt
     2941 taatagctat aaaataaatt ttatagcata aaagaattta ttatatttta atgtatctta
     3001 ttaaagttta tttagaatta tgaaccaact ttaataagat gtggcgcatt taaaccgatt
     3061 attattatac aataatatct taaatattac aattaataac attaatttat tatccataaa
     3121 aatatattaa cttatattat ttaataattt attgacagta ttaaaattaa attatacaat
     3181 aacagtgtaa attaacttaa caccgttaag caaagaggtg attatatgag tatcaaagaa
     3241 agacgtaaaa atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt
     3301 ataaaccaac atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat
     3361 tctccaacta caatttatct ttactacaag gacaaagcag agattatcag cgatatgacc
     3421 aataaacttt ataacacagt agaaagtaat gctattgaca ttatgaataa ttgctcatca
     3481 ctaccaatag ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt
     3541 gaaccagaga tggcaaaagc tatcatgcat agtagaatga atattatatt tgcatctgaa
     3601 aatacaaaca atactcctag caataatggt ataaaaatgc ttgataaatt cctttccatt
     3661 ggaattgaac aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt
     3721 gcattgttag gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt
     3781 tctcaactta tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg
     3841 caacaaagct actaataaaa agaaaaaata tgtaaaaata tctattgctc tattctttgt
     3901 atttatatgc attattattg taattgtaag caacttaaag ccagaaaact tggtagtaaa
     3961 agatatagat attaataatg ttaaaaatgg catttatact ggaagtgctg ataataactt
     4021 ggtaaaagct actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt
     4081 aaaacacgac catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca
     4141 acagtcttta gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa
     4201 agctgtagaa aatgctttaa gaaagggaga ataattatga atactattat tatatattca
     4261 agtaaatatg gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat
     4321 gtcactattg ttgatattaa taataataat aaaattgagt tatcaaaatt tgataaaatt
     4381 attattggaa gttctatata tgttggttca gtttcaaaaa aaatacaagt gctatgtaat
     4441 gataatgtag agttattaaa caaaaaacaa gttggcattt tcctttgctg tggcttttct
     4501 gaacaagcag ataaatattt aaaatcaaat tttccatcat ccttattaga aagtgcaaat
     4561 gctattggaa tttttggtag tgaagctcga ttagaaaaaa tgaaattttt agataaactt
     4621 attatgaagg ctgtttcaaa aggtaattat gacagtttta gaatatcaca ggataatata
     4681 gataattttt taataaatct taatagctaa aataaaacta attatttcta tgataaattc
     4741 ttttatttat aagtaatatt gctcaaaata gtctttaaac tttatcaaaa ttttattttt
     4801 cttagtgaaa tctaatgaaa ctagctctta atatatgctt tattaatcat aacaaaaaga
     4861 gagtgtctaa tgaactaaaa agttcattag acactctctt tttgtatgaa atcaaatata
     4921 tttttatcat ttcaaaagag aaaaatacac ttgtctctat gtttgacaca ttctcttttt
     4981 ataatataat atctaattta aaacctattt caaatattga catatactta aaccatagac
     5041 ttacaaaatc aattcatatt agcttaaaat ataatctttg ttactcaaat tcctatacac
     5101 aaccattatt tactttgata ctttaaaaca tcttccctat tttttattaa attttataat
     5161 aatagtccta ttaacgcaaa tactataaaa agagtttaac tttatccatt aaataaattt
     5221 aaattaaaac atatagaaat ggaaggagaa caatttatga taaaactaat cggtatattg
     5281 atagttgtaa ttggatttat acttaaaatt gacacattat ttactgtatt attagcagga
     5341 gtggcaacag gaattgttgc aggattagat tttaatcaaa tttttacaat tttaggagat
     5401 tcttttgtct caaacagagg tgtatcatta ttcatattaa cactgccagt aatcggtgta
     5461 ttagaaagat atggtttaaa acaaagagct gtttctttaa tagaaaaatt aaaaagactt
     5521 acaacaggaa aagtacttac tatatacatg atagctagac aaatagctgg tgctctatca
     5581 ataagaatga gtggacatcc tcaatttgta agaccacttg ttaatcctat ggcacaagct
     5641 gctggtttaa gtaatagtga tgaactaaaa gaatcggatg aagaagccat aaaggcatta
     5701 tctgctgcat cagaaaacta tggaaatttt tatggtcaaa acttatttgc aggtagtagt
     5761 ggagtattac ttatagcatc tactctaact caatttggat acaatgtaac tggacttaat
     5821 attgtaaagg ctagtactat tatggcagta atagcacttg tagtggctac aatccagttt
     5881 accttatatg ataaaaaatt aaacaaaagt cataaaaaat agttttattt aggaggaata
     5941 aaaatgcaaa attttataaa catatcattg gaaatctttt acgcactaat gggattttta
     6001 atgattgtta tagcatataa gtcttttact accataaata acaataagaa atatggtaca
     6061 tcattatttt ggatacttat atcactgcca ttcatatttg gtagattgat tccagccaat
     6121 ataataggaa taatattaat attatctagt ttattaacac tttctaaaca agttgtattt
     6181 gcaaagtatg aagaaccaga tgaaaatttt ggaaaagaac aagcagacaa acttaaaaat
     6241 aaaatattta taccatcatt aatattagca tttgctgcag tagtagtagc tatgagcctt
     6301 tctaattttg ataattcttc tcaatttgca ataggtgtgg gttcaataat tgcattaata
     6361 tcagccctta taataactaa agcaaaacct gctacatcag ttcaagatgg ctcaagatta
     6421 cttcaacaaa tgggacctgc tagtatgtta ccacaacttt tagttgcatt aggagcttta
     6481 tttactcagg ctggagtcgg cgaagttatt tcaaccatga tatctggtgt agtccctgct
     6541 gatagcagat tatttggtgt tatagcttat gtattaggta tggttatatt taccatgata
     6601 atgggaaatg catttgccgc attttcagtc ataacggcag gaattggtat tccttttgta
     6661 ttgtctcaag gtggaaatcc tgctataatt ggagcattag cattaacagc aggatattgt
     6721 ggaactctac ttactccaat ggctgctaat tttaatatag ttccagcagc cttgctagaa
     6781 tgtaaaaatg attatattgt tataaaatat caagctccag ttgctttggt attaattata
     6841 gcccacatat tagtaatgta ctttttagga ttttaatata attaggaggt aaaataatga
     6901 aaattttgtt aacaggattt gacccatttg ggggagaact aataaaccca gctcaagaag
     6961 cagttgaaag ggtcaataat aatataaacg gtgcagaaat cataaagata actataccaa
     7021 cagttatgac aaaatctgtt gaagctatag ataaagctat tcaagagcac aatccagata
     7081 tagtaatatc agttggacaa gctggaggta gatttgacat tacacctgag agagttgcta
     7141 taaatataga tgattttaga ataaaagata atgaaggtaa ccaggttata gatactatta
     7201 taaaagaaga tggtgaacct gcatattttt caaaattacc tgtaaaagcc atggtaaaac
     7261 atatgaatga aaataaaatt ccagctagtg tttcaaacac tgcaggaaca ttcgtttgta
     7321 atcatgtgat gtatggaatc ctttatatga tagacaagaa atatcctaat ataaagggtg
     7381 gatttataca catcccatat acaacttcac aggtaataaa taaaaaaaat acaccattca
     7441 tgtctttaga agaaatagta aaaggtttag aattagctat agaagcttgt attatataca
     7501 aagaagatgt aaaagaaatt ggtggtgaaa tttcataata aattttttag tattttataa
     7561 attaaaaata ctaactatta atttaaatat agactgtgag tagattattc aaacaatttt
     7621 actcacagtc tttttatggc atctacagat acaaaattaa ggtctattcc tctctcccca
     7681 ctcacacatt gcatctaaaa ttggaatcag agattttccc ctttctgata aactatattc
     7741 taccttagga ggtatttgag gatattcagt acgaataatc aagttatctg cttctaattc
     7801 ttttaatgat aaactcaaag ttttatagga aatagtattt atataacgtt ttaatgcatt
     7861 atatctgaca acttcaaatt ctacaagaca gtaaagaatt atcattttat atttaccact
     7921 aatcagtgaa agtgtataac taaatcctgt ttcacttaaa tctgtatttg atatacagtc
     7981 tatactcata tattacactc tccttttggt tagtattgaa caaaaatgtg cgtacttgtt
     8041 tttagaataa tttataataa aattatagtg taagaataat tgaaagtcaa ttttataata
     8101 ggaggtgtat ttactaaaaa aagagttatt ttaaatagga ttctaagatt agacatttct
     8161 atataaattg ataatattca tgaaaatcct attttataaa aaataatatc tcttagaaaa
     8221 tctattaaat aaaattcatt ggaggaaatt tataatgaaa gttttattga taaacggtag
     8281 cccaaaccaa tatggatgta cttatacagc actaaatgag attactaaag tattgagtaa
     8341 acacgatatt aaaacagaaa tactgtattt aggtaaagaa gcaatcccag gatgtattag
     8401 ttgcgcatcc tgttttgaaa ctggaaaatg tattcgaaat gataaagtta atgaattgat
     8461 tgaagatctc gataatattg atggtattat tattggttct cctgtttact tttctagtgc
     8521 aactggtcaa ttaacttctt ttcttgaccg tctatttttc atagctggaa gtcgcatggc
     8581 tacaaaatta ggtgcttcag ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga
     8641 ccaactaaat aaatattttt caatcagcaa tatgcctatt gtatcatcac agtattggaa
     8701 tcaggtacat ggctttactc cagaggatgt tatgaaagat gaagaaggat tacagactat
     8761 gcgaactcta ggagaaaaca tggcatggct tcttaagtgt atatcagcag gtaaaaaagc
     8821 tggaattaaa gaaccacaat atgaagaaac aattatgact aattttataa aataaataaa
     8881 aaatgcacta atattaatta aaacaaaaca ctaatttata aataaaataa gtataaatat
     8941 aggataaaaa acactaaaac agaggtattg aattttatat caaacctcta ttttattatt
     9001 acatt
//
LOCUS       DAEPOG010000151         8995 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEPOG010000151 DAEPOG010000000
VERSION     DAEPOG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.151"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..214)
                     /locus_tag="KR432_002937"
     CDS             complement(<1..214)
                     /locus_tag="KR432_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641453.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNN"
     gene            complement(715..1695)
                     /locus_tag="KR432_002938"
     CDS             complement(715..1695)
                     /locus_tag="KR432_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641454.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(1859..2731)
                     /locus_tag="KR432_002939"
     CDS             complement(1859..2731)
                     /locus_tag="KR432_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0641455.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(2895..3446)
                     /locus_tag="KR432_002940"
     CDS             complement(2895..3446)
                     /locus_tag="KR432_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0641456.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(3466..4368)
                     /locus_tag="KR432_002941"
     CDS             complement(3466..4368)
                     /locus_tag="KR432_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0641457.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(4383..6755)
                     /locus_tag="KR432_002942"
     CDS             complement(4383..6755)
                     /locus_tag="KR432_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBH0641458.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(6974..7663)
                     /locus_tag="KR432_002943"
     CDS             complement(6974..7663)
                     /locus_tag="KR432_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641459.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(7904..>8995)
                     /locus_tag="KR432_002944"
     CDS             complement(7904..>8995)
                     /locus_tag="KR432_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0641460.1"
                     /translation="YLFSVYYKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQ
                     IYSNLIIILIYLVFSFFRKSIKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFG
                     SLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLS
                     IQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILD
                     IIINEKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENIN
                     KYIRIKGTITKSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYN
                     GELLFENSIDKFILNIYIPLEQDTDSWGD"
BASE COUNT         2918 a         1580 c          847 g         3650 t
ORIGIN      
        1 tattattaca ttttccacaa caatactcat atgtctccac attatttgga atatttacaa
       61 atacaaattc tttacagttc tcacaatact ttaatataga tatagttcca caagtgtaac
      121 attcaaatat tacacaatcc tcattttcct ctactatttc aaacttttta ctatgttcac
      181 cacactctgg gcacttgtga ttatttacat tcataaatac tcctccttat tagtatagtt
      241 acatttcaaa aacttaatta atgtattcta tattacttat tatatattct ataataaatc
      301 atattctaat ataataaaac aaattaaata aaaaaaaacc ataaatttta aatttatgga
      361 ttaattcttg taatgataac tctatttgag gatgtgaggg agggttatca ttacaatagg
      421 gtattatatt atggttttta agctagtctt tctacacctc atatgataca tatgctatat
      481 gaattttata tgatttttac ataaatattt tatgaaattt aaatttttta gtcattttga
      541 aatttgtaaa aagttctcta aaaatccaca gcatcataaa cagccaaaat ttaagtaaaa
      601 catcataaaa aaatttgtcc aataatttta acaagtcatt tcacctcatt ataattattg
      661 acacaaactt aaataaacaa tctataaatc ttatataaat cttaaacttt acccttattg
      721 atacacaatt tctagtttgt caattttatt ttgcatctct aagaaaattt cttcaacaga
      781 attgttaaat atattgccta aatagactct tttatctttt tgaaatgaat aagattttac
      841 actaccatca gaagaaatat ataaatgact ccactctgct ccatattttg aaaacctctc
      901 ctttgtatct ttaaacctca tatcttctat ttttattgct ttttctctgt ctcctcttac
      961 tacagtgtga ccccttttta ttaaactggt tatttttata tcttctttta atttaatacg
     1021 tgtatcatca ttttttgaat atactttcca tatcacttta tgacgataat tatctaattc
     1081 ttttattaag cttctatcaa tagtaaagct atctttagtt accacaatta aatcaaaatg
     1141 gtctaacata tattgaaata tttctctcat acttttattt tttaaaatat cctcaccaat
     1201 aacctctata aatctacagc ctttctcata catgcagttc aataaacttg tgatttcaga
     1261 aaaataatca tttgtattat tatctccatc tatttctata gtaaagtaca aaggagtaat
     1321 gtatgattta tttcctgtta tctcaaatac acttgatagt gtctgtatat catcttcaaa
     1381 cgttaagtat acattctcat aaaacataaa aaattccata actttttctt tcatattatg
     1441 gatatttcta tatctattgg ataatatatt tattatatcg tcagtagtat ttttcccatc
     1501 acattcagcc agtataacaa tttgcttcca acttaagcta tccacatttc cgatactttt
     1561 tttatatcca tcttcttcat cataaatagg accaattata atgttttctt ttggccacag
     1621 atgaacccta gttccatctt ttaatttcgg atatttattt tttacagact cataaaatct
     1681 acttgtatca cccatgattc tttactcccc cccatagttt aaatattcta tgctaaaaat
     1741 agcactttat ttttattaat tcaacaatta ttcaaaattg gtcattatat atgtttaaat
     1801 ggatttaaca tcaaccttat ttatatgtta ttgatgttta tatatgttat ataaaatctt
     1861 atttctcata cttcaatgta atcttaacta tctctggttt acaattaagc ctaattaaaa
     1921 cttttgagcc tcctaagcct ttatttaaaa tcatagtgct attatctttt tcatattttc
     1981 cccagtcata cttaggaaag aactctctat ttggagataa tacacctcca acaaaaggta
     2041 ttcttattat acctccatga acatgtcctg ctaaaaccaa atctgctccc cattttgcat
     2101 atttttcaaa gaaaaatggt gtatgtgcta aaagaatgtt atattcattt tcattaactt
     2161 tacctagatt gtcttctaca aaattgtcac ttagttctaa ttttttgtta ttagcaaata
     2221 aatatggata gcattccaat ggtataatta aaccatataa attaagatgg ctattacctc
     2281 tatatattct tacagattca ttttccatat ttattatttt ctttgaatat agttccttaa
     2341 aataatcctt atatagatgt ttatactttt ttattaaaga tttttgttca tgatttccaa
     2401 ttatatgata tacttcatat ttttcaacca aattatctat taaatctaat gcaacttgaa
     2461 aatttttatt ttctccatct actaaatctc ctgttataaa tataacatct gggtttaatg
     2521 attcaatttt atctatcaat accttattat ttttatcaaa caccttatta tgtaaatcac
     2581 tcacttgaac tattttaaag ttatcaaatt cctttggaat ttttttgttg aaaatagtat
     2641 attctgtcgt ttctatatgc tgagtttcat acttatacag aatatacaat aatattaata
     2701 ctaatactgt cattgaaacc tttataatca tattctcttc ctctttattc tattttgtta
     2761 atattattat aatgctttta tgtttttttt aaaacaataa catcatcaca tacatataaa
     2821 tcttttcgac attataaaaa taagttatcc ttttctaaaa ttttcattta ttttagaaaa
     2881 ggataactat aaaattatct ataaacctct atattgtgaa atatagtttt tcctttattt
     2941 aaatttttat atatatgctc taaagttcca tcaaacctaa tagaatgtcc agactctaaa
     3001 atataagttt tcccatccac attaaggtca agtttacctt ctattacgag tacatactct
     3061 tctaaaacat aactatgtgt agatgaaaca tgagtacagt tttcctcaag ctcaattata
     3121 aaaagttcaa accttctttg agggctaaaa gggtatattg gatataactt catgttccct
     3181 ttttgctcaa caataggctc tacttcttct ttctttataa cctcataggt atttgtattt
     3241 tcatttaata gtgtagtaaa atttatttta aatccagatg aaattttcca taaagtagat
     3301 actgtcggac ttgattcacc tctctcaatc tgtccaagca ttgctttact gacacctgtc
     3361 atctttgaag ctttgtctaa acttatgccc atttcttgtc tcaataacct taaattttct
     3421 cccacattag gaaatttatt atccatgtta aaatcctcct gatagctatt tgtattctat
     3481 gatttttact cctgtaagtt ctactatctc ttttaatttt aacatatgat tgttacaaat
     3541 ttcatcaaat tcatatatat aatcttcaag ccctaatgcc ttatatttat cgattccaaa
     3601 tttatgatat ggaagtaact ccatcttgcc tcctggaaca ttttgtttga caaataatgc
     3661 agtatttctt atattttctt cactatcatt tactccataa ataattggca ctcttataac
     3721 catagattta ttcaactttg aaagcctaca tatattatct aatataattt tattacttac
     3781 tcctgtatat tctttatgaa tattatcatc catagatttt atatctacaa atatgtggtc
     3841 tattttctca aatacatcat ttaccttatt ccaatcaaaa tatccacaag tttcaattgc
     3901 aatatttatg cctttatcat aaaaaatatc aactaattct cttaaaaaat caatctgaag
     3961 tgtaggttca cctccagaaa aagttatccc tccattagat tcaaagaaaa atataaaatc
     4021 cttttcaact tcctctacta tttcttcaac actcattttt tcggtcatta tacaaatggc
     4081 atctttaaga cataccttga tacactctcc acataaatca catttattat ttatttgcgt
     4141 tctatcaaat agtgaactta tattttgagg acaaacatct atacatctat tacaggatac
     4201 acatttatct ttcataacac ctaattttac tatgttactc caggaatctg gattagaaca
     4261 ccacttacat tttaatttac atccttctaa aaatatagta gtccttattc catctccatc
     4321 atttactgaa aaatgttgta cttttacaac tctacctttt ttactcataa tctacctcat
     4381 aattaaaaat tgctatgtga atttctagct attatagcat cttgcatttc tcttgataaa
     4441 ttaacaaatt gagtactata tccagcaact ctcactaata aatctttata ttcttctgga
     4501 ttttcttgag cttttaatag agtatcattt gatatcgtat taaattgtac atggaatcct
     4561 cctaaagcaa aatatgattt aatcatagat gttagatttc taagtcctct ttcagtttcc
     4621 accaaatctt gattcagtct taaatttaga agtgttccac cagtatgaac atcatgattt
     4681 atttttgatg cagatttcat ggctgctagt ggacttgttg tatcagtacc aacaaacgga
     4741 gaaactccct ctgtcaaagg ttttgtcgct tttcttccac aaggagttgc acctacgatt
     4801 tttccagttg gtacatagtt tgatataccc ataaatgcac tattaaattt agagccaaaa
     4861 atatccttgt attttctagt ttcagtataa taaaaatcag aaacttcaat tgctaatgaa
     4921 tctacataat cattatcatt tccatactta ggaacatcca acgcaagttt tcttaattct
     4981 tcataacctt cccaatcatt atttagtgct tttgttaatt cttctagcgt agtcacctta
     5041 tcctcaaata ctaatttttt tatagctgcc aatgaatttg aaactacacc taaccctata
     5101 ccagttaata caggacctat attatatttt gctcctcctt tacttaaatc aactccctta
     5161 tccaaacatc catctacaca agtagataaa aatggtcttg gtaccatttc tttatgaatt
     5221 tgttgagcaa cagtagttcc tataacagaa ctttttacaa gatatgctaa ttgctttaaa
     5281 aactcatcct taagttcttc aaaagaagta aattctgtga tattttttgt atctaacccc
     5341 attttttctc cagttaatct actcttacct tcattcatag catattctaa agctgctgca
     5401 aaattgatat ttacggctga agtccactgt cctgtttttc taaaatgagg aactacacaa
     5461 ccacagttac tccaatctct agcatcttct gcatcatatc catcttgtaa taacatttgt
     5521 gcccctgcac tatcgctgtg tatagcaggg aaaccagttc cttgttttac caatttagca
     5581 actgccatta cgaaattatc tggtgctcct tgactcactc taacacttaa tcctggctgg
     5641 tgagttttta cactttctgt agcctttaaa cacatatatg agatatcatt cgttccatca
     5701 gtaccatttc tctttttacc accaacagta agattttgga attgattata tcctgcaaag
     5761 taatttgctg tatttgctga tatagtccat acccattctg ataacttaat ccaaagtgct
     5821 tctatcaatt cttgagcttc tgattcagtt attttacctt ctctagcatc attttcataa
     5881 tatggataca tatattggtc aaatctacct aaatttaatg ccaatggatt ttctgataga
     5941 ataccaccta attgaacaaa ccatacaaat tgtattgctt cataaaaatt agttggaggg
     6001 tttgctggta cattcataca tatctctgaa atttttataa gttctaactt tctcttttca
     6061 tctgtttctt gcttactcat ctcttttgca agatttgaat atctttgtgc caatgtcata
     6121 ataccctctg caactataga aacagatttg taaaaatcta tcttttcaat attttctgaa
     6181 actgatatat ctaattcttt aattttttta tctgcatctt ccttaatccc tttatatcct
     6241 tttacaaaca atatatcttg ataatctggt gttacttcac caactgcctg tctccactta
     6301 gaatcattat ctattattcc tgtatctact aatatcttag ctgtatcttc tgggattctt
     6361 gctaagaaaa cttcttccaa agattttccc ttccaatatg gaaatatttc ttttcttata
     6421 aattctattt gttcttttga tattacatat gggtcttgag ttcttttatc aaaagtatcc
     6481 atttctttgt ctacccattg ccaagaaaac tctggtgtca atatccctgt tcttctaaat
     6541 tttcctgctg tacctactat taattcattt tcaaagattt ttataggaag ttctttacat
     6601 gtctccaaaa aagctttagc tcttcttata cagatagctt ctccctcagt ttttttatgt
     6661 gattctgtaa aaattctagc cctttcacaa catatcatag gtttagaatt aatatattct
     6721 tgtctaaatg cttctattct attagtttga ctcatttaaa atcccccttt ggataatata
     6781 tactacattt ttgtatgtta taacatactt taattatatt atactgtcct ttagcataaa
     6841 atggaagcaa aataactttt tttttcatct accttgaaca cctatatatt aatacttttt
     6901 ttgttaaatt tataaaatct aaaaaaattt tctatgatat attttctata ttgttatagt
     6961 tatttctaat atactaatga tttgaaatat caaatttgta tactagactt agttttaaaa
     7021 ccttagttga ttgtttaact ttgttaactt caacatgtat atccttcata taaggaagct
     7081 ccacaacacc tactccctta ttaaattctt cttcaaccat agaaaaaatt tctttctcag
     7141 ttaagttcgt attagtcaat gatttaaata aattgtatat aaatttaata tatttatcac
     7201 tctcagataa cttatcatct tctggatact ctttagcaca gagaatatct attgttactt
     7261 ttccttcttt atcgtcttca gacatagaat agaataaact tcgaatattt ttttcaggtg
     7321 gtttttcagc accatttata gatactacaa agctaccatc tgattttgag tcagtaatac
     7381 tataaccact gtctattata gctttcgaaa atctttgaga taaatctttt tttacttctt
     7441 ctctaccttt cgcatcatta atcttttgtg agtaattatt aaatagctcg atagattcat
     7501 cttttttatc ttcaacttgt tctttcaatg aatctttact aatttgttct gagctactta
     7561 agttcttatc agatacagga gacatacatc ctgccatcaa tgctaagcta agatttaaag
     7621 ccacaattat tgccactcta tttatattcc tttttacttt catacacaat cctcccatca
     7681 cactaaccgc tctaaagtat tattcattgc attagaatat atttatattt tctttatatt
     7741 aaagatttat tattcaatac atatcacctt cctatccaat tcttataact tactattagt
     7801 aaaattataa gctccttaat acagaaataa ggatgttatc ttttgttatt aaaagataac
     7861 atccttattt ttttgtatca atacatatgt cccaactggt aacttaatcg ccccaactgt
     7921 cagtatcttg ctccaatggt atatatatat ttagtataaa tttatcgata ctattttcaa
     7981 ataataattc tccattatat ttttgtaaag aagatttaat actctgaata cctattccat
     8041 gtacgaactt atccattttg tttgttaaca atatattttt attaaaagta agctgattta
     8101 gtttactatt ttcacattta agtacaaaaa atgactttgt tatagttcct ttaattctaa
     8161 tatatttatt aatattttca tccactattt tatcacatgc ttctattgca ttatccaaaa
     8221 tattagaaaa tatgcttgac acatctatag gttttacaaa atttactttt gaaaaattta
     8281 tagagcaagt aaatttaatt ccttttttta aacatatttc actcttttcg ttgattataa
     8341 tgtctaatat catatttcct gtattataga tatatttaaa tgtttttatt tcatctttta
     8401 aattgttaac atattcatta atttctttgc tattgtttct aaggttgtct atacaataaa
     8461 tgtggttatt tatatcgtga tatagtttct taactcgcat atgagattct tgaatactta
     8521 aataatgcgc atattgcata tctaacttat tttttattaa ttcagcttga gattttaatc
     8581 tattctcttt tattatttct ttaaacaata atataagaaa tatactacta gctcctacca
     8641 actttggatt aacaaaatta tcaaaatagt atccttctga atgtaatgac ccaaaccgtt
     8701 ctaatttttt taatacataa tttacaagca tattcattgt attagctaat aaggcaaata
     8761 ataaatatac ataatttctc ttatcttggc taattaattt tatagacttc ctaaaaaagc
     8821 taaatacaag atatataaga ataattatta aattactata tatctgaatt tttatagatt
     8881 ctaagtgagg tactaataag gattctttag atgaaataac attacctgta attgcaaaaa
     8941 atagtattct atatataaca gcatccaaac atttataata tacactaaat agata
//
LOCUS       DAEPOG010000152         8969 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEPOG010000152 DAEPOG010000000
VERSION     DAEPOG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.152"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            173..1531
                     /locus_tag="KR432_002945"
     CDS             173..1531
                     /locus_tag="KR432_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0641461.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            1637..2431
                     /locus_tag="KR432_002946"
     CDS             1637..2431
                     /locus_tag="KR432_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0641462.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            2424..4730
                     /locus_tag="KR432_002947"
     CDS             2424..4730
                     /locus_tag="KR432_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0641463.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            4869..6146
                     /locus_tag="KR432_002948"
     CDS             4869..6146
                     /locus_tag="KR432_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0641464.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            6329..7051
                     /gene="yqeC"
                     /locus_tag="KR432_002949"
     CDS             6329..7051
                     /gene="yqeC"
                     /locus_tag="KR432_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH0641465.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7158..7727
                     /gene="mocA"
                     /locus_tag="KR432_002950"
     CDS             7158..7727
                     /gene="mocA"
                     /locus_tag="KR432_002950"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH0641466.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            7724..8743
                     /locus_tag="KR432_002951"
     CDS             7724..8743
                     /locus_tag="KR432_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBH0641467.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3529 a          929 c         1686 g         2825 t
ORIGIN      
        1 aaagaatacg tgtatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa
       61 cttaaatata tgttggagta tagttgatat ttatgaacat cattaattta atatattaga
      121 tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc
      181 aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt
      241 agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc
      301 aaatgcatct ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt
      361 tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt
      421 aggaggagga ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc
      481 tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag ctggtatagt
      541 aataattata attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc
      601 aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc
      661 tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt
      721 atctcttatg atattaggat taaataagta tggaaaagga ttatttaaag atgcatcaat
      781 attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc
      841 ttcaattcag gagtttactt tggttgcgct tccaaaacca cttgcttttg ggctagatat
      901 aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat
      961 gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact
     1021 ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac
     1081 tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa
     1141 atttgtacta gctattggag gaattggatt atttttggca ggaatatcgc ctttattagc
     1201 taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc
     1261 aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag
     1321 catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct
     1381 tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc
     1441 aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga
     1501 taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat
     1561 atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt ttaataagtt
     1621 aatgatgggg gataagatgg ttactataaa ggaatattca attccacaga gtttagaaga
     1681 agcatataaa attttaattt ctaaaaaaaa taatgttatt ttaggtggat gtggatttat
     1741 aaaattaagt aataaaaata taggaacagc gatagattta aaagatataa atctaaatta
     1801 tataaaagag gatgaaaaaa atattttgat aggtgcagat acttcactta gaagtttaga
     1861 aattgataaa actataaaaa attactgtag tggaatactt tcaagcgctg tttctaatat
     1921 agttggtgtt caatttagac aaggggctag aattggtgca agtgtatttt caaagtatgg
     1981 cttttctgac ttaataccag cgcttttggt tgttggagcg aaggtgagat tatacaataa
     2041 aggaatacta gagttaagtg agtttttgga tagtgaatta agtagggata tattggtcga
     2101 agtaatacta ccaaaaaaag atgcgatagc tgtttttgac tcaataagaa aatgcacagg
     2161 agatttttct gtattaaatt cagcaatgct aaaagaaaat gatacttaca aaatagcaat
     2221 tggggcaaga cctcaaaaag ctagattagc acttgaagcg agtaatatat taaataaaga
     2281 aaaagatata gataaggcat ctattgttgc tagtaaggaa cttacttatg gaagtaatat
     2341 gagagctagt aaagagtaca gaaaggatat ggcaagtgca ttagttaaaa ggatgtataa
     2401 tgctattgag gagggaatgt ataatgatta ataaggggat aagaaagata gatagtacag
     2461 caattgtcac aggaaaacct ctatatactg aagacctcat tatgcataaa gatgttttga
     2521 cattaaaact acttagaagt ccacatgctc atgctaagat aaagaatata gatacatcaa
     2581 aagccgaaaa aattccaggg gttgtgggta tttatacgta taaagatgta ccacaggtca
     2641 gatattgtgt gccaggtcaa acagaaccag aagcatcacc ttatgataga gttttacttg
     2701 aagatacagt tagatttatt ggagatgaag tagctttagt ggctgctgaa gatgagaaaa
     2761 cagcacttaa agccttaaaa atgataaagg tagattatga ggttttaaag cctgtgtttg
     2821 atgcaagaga agcagaagga aatgaagttg ttatacatcc agaaaaagac atagtagagc
     2881 catacccttt tggattggat gcaaaaaata atatagtatc aaaagaattt tttgaatttg
     2941 gagatgttga agctaagctt aaagaaagtg attttgtggc tgaatcttca tacaaaaccc
     3001 aaagtcaatc gcattgcatg atggaaactc ataggtcata ttcatataaa gatataaatg
     3061 atagaattgt tgtagtttca tcattacaat caccacacaa tgtaaagaga ataattagaa
     3121 aagtattggg tataccatct tcaaaaataa gagtaattaa acctagaata ggtggtggat
     3181 ttggaggaaa gaatatagca gcaacagata tatttactgc atttgttact atgaaaactg
     3241 gtagaccagc aaaatttgta tttacaagaa gtgaaacttt tgtaggttct aatagtagac
     3301 atgaaatgtt ttttgatgtt aaagttggtg cagataaaga aggaaattta aaagcaatag
     3361 agctaaagtc attaaacaat acaggtgctt atggtgggaa tggtgtctct gtttcttccg
     3421 aatctggaca caatacctta ccaatatata atgatattga tgctgttaga tttaatgcta
     3481 gaacagtata tactaatagg ttaccagcag gagcacttag agggtatggt gctacacagg
     3541 gaatatttgc aatagatagt gctattgatg aattggcaca taagattgga atggacccac
     3601 ttgaatttag aattaaaaat ataatagata ggtataccaa tggaaaagca gctgaagacg
     3661 aaatattaag ttgtgagctg agacaatgta tagaaaaggg caagaaatta atcaattggg
     3721 atgataaata tccatgtaaa gatataggaa acaacaaagt aagagcagta ggtatggcag
     3781 tagcaaatca tggttctggt ataccgcgtg tagatatggc aacagtcact ataagaatgg
     3841 atgaagatgg cacatataaa ctattatctg gtcttgctga cttaggtcaa ggggctgata
     3901 caattcaagc tcaaattgct gcggaagctt taaatactac tgtagataga atttctattt
     3961 atacaggtga ttcagattta tgtccctatg atacaggagc atttgcttct tgtacaacaa
     4021 cggttacagg aaatgcaaca ataaagacag caaataaatt aaaagatata ttactttcag
     4081 ttgcagagtc aaagctagga tttaaaaaag aagatttaga gttgggaaaa gacagggttt
     4141 ttgttaaaaa cgataatagc aagtttgttc tattagagga attgggtaga gaatctgttg
     4201 gaggaatagg atttgctagt gatgatgtac cagtagtttt ggaagcaaca gaatcttttg
     4261 gcatgttgaa aaaaataaaa ccatttatag caggatttgc agagatagaa ttagataaaa
     4321 atacaggcga atataaggtc ataaattttg catgtgtacc tgattgtgga acagttataa
     4381 atccaacttt agctagaata caagtagaag gtggtgtgat gatgggtata gggtttgcaa
     4441 tgtatgaaga ccctatattt gataaagatg ggagattatt aacagatagt ttcctacagt
     4501 ataaagttcc tacaaaaaaa gaagttggaa atatattggt agattttgct gagaatttcg
     4561 acccaggagg tccttatggt gctaaatcat taggagaaat agttatacat acaccagcgc
     4621 cagctatagc aaatgcaata tttaatgcta caggagttcg tttaagagag cttcctatga
     4681 cttttgaaaa agtttatatg ggtatgaaaa ataataataa acaattataa taaataataa
     4741 ggtgtataaa agcaagtaaa tttaaattat tttgtaaaat aaagtaatta aatggacatt
     4801 atatatggtg ttttgtaaaa ataccgtata taatgtctag tagagtatat tctaaaggag
     4861 gtgagttcat gtcaaacaga ataaaaaata tacactatgg aactcagaga caacaattaa
     4921 aaaacttaac taaaaaagaa tatcaagctc tacgagagat gtgcttctta gctaagaact
     4981 tatataatgt tggtacatat aatgtaagac agcattattt caatcaagga gaacatctta
     5041 actatgaagc taattatcat gtatgcaaag aaaatgagaa ctataagctt ctcaatagta
     5101 atatagcgca acaaatatta aaagaagtag acagttcgtt taggtcattt tttgggttaa
     5161 ttaaattagc caaagaaggg aaatatgact ttagagggat aaaatcacct aaatatttag
     5221 ataaagaagg attttttagc ataataatag gtcaaataag aatcaaagaa gatgggattt
     5281 taaatgtacc tatgtctcca gcatttaaaa aactatatgg gaaagtaagt ataaaagttc
     5341 cacaaaatat actagagaaa aaaattaaag aaataagaat aatacctaaa cataaagcta
     5401 ggttctttga aattcagtat tgttatgaaa taccgaaatc agaggaagtt tcaaatcaaa
     5461 aaaatgcact agctatagac ttgggtttag aaaatttatg tacatgtgtt accaatttag
     5521 gtgatagttt tatcatagat ggaaaaaggc taaaatctgt gaatcagtgg gcaaacaaac
     5581 agaatgataa aatagaatcc ttaaaattaa aaaagaatat aactgcaata acaagaaaac
     5641 aatttaaagt ttggaataag agaaataatc aagttaaaga ctatgtaaat aaaacctgtt
     5701 tttatataat tgattattgt gtaaaaaata atattggaaa tttaattgta ggatataatg
     5761 ctaagttaga aaaaagtgat aatatggaga gaaaaatata tagaaatttt ataagaatcc
     5821 catttggaga gataaaagga aaattagaat atctatgtaa aataaaaaat atcaatttta
     5881 ttaggcaaga agaaagctat actagtaagt ctgatttttt tgctgatgat tttctacctg
     5941 atatcaatgt ggacaatgtt aaaaaatatg actttaaagg ggaaagaata acacgtggac
     6001 agtacaaatc cagtgttgga atcattataa atgcagatgt aaatgcgagt cttaatatac
     6061 taaaaaaatc acaagtagtt aatttatcta atttacagaa taatcctcaa atattaaagc
     6121 aaccacagag agtaagaata tcttaattat atttaaaaga taaactgata gcgtaagctt
     6181 acagcttagc ggcgaattgg atccgccttg ttgtttacaa cagaatctat ttactttcga
     6241 gtttatggaa tttcaaaaat cacttttact aaaaaggtct aactataata attaagagat
     6301 tagaaaactg gttataagag gtgaaagtgt ggaactatca aatataatag aaaagaatga
     6361 aataataact gtagttggag caggaggaaa aacttcgttt ataaactatt ttgcaaattt
     6421 ttatagagat aaattgaaag ttcttcttac aacaacaact aaaatatatg taccaaacga
     6481 ttatgacaat ataattatta caatagatgg aactgttatt ccatctattt gtcatggaat
     6541 aacagtctgt ggcagttata taaataatga aaataaatta gtaagtattg attcctctat
     6601 tttggatgaa attgtagacc aatttgattt agttttaata gagggagatg gctcaaagag
     6661 aaaaaaactt aaaggttgga atgctaaaga accagtagta tatcataaaa ctacaaaaac
     6721 catagggata ttagatataa catcatttgg aatgaatatt aatgaagaga atatacatag
     6781 agtagaaata tttaaaaaaa tagcaaattt agatacaagt agtattaata gtagttctac
     6841 agtaagtatt gaaaacttaa aaaatatagt attaaatcca aatggattat ttaaaaatta
     6901 ttctggaaaa agagtattat ttattaataa agttgaaaat gaaaaatata aaaatttagc
     6961 tataaaatta atagaaaata ttaaagaata tgaaagtgaa atagagatat tttatggaag
     7021 cgtaaaacaa aaattttgtg taagatattg aagtattaaa atagtttata tgatattata
     7081 tatatagata atatcaatag agatattata aaaatcatag aactatcaat atattataaa
     7141 taccaaggat gatatatatg ataaatgcag tgataatggc atcaggcttt gccagaagaa
     7201 tgggagcaaa taaactttta ttaaattata aaggctgttc tataatagaa catgtattta
     7261 gagaagttag caagattgat tttcatcaag ttgtagtagt aagtcaatat gaagaggttt
     7321 tgttattgtg taataaatat ggttttgaat atatagataa taaaaatgca aatataggtc
     7381 aaagtgaatc tataaaatta ggagttttaa atagctcaaa atgtgatggt tatatgttct
     7441 ttgtaggaga ccagccattt attgataact tatatataga taaaattata aatactttta
     7501 aattagatag agattttata gtaattccaa gatacaaaga tatgtgtgga aatccagtca
     7561 tattcccata tagtaagaaa gaagaattgt tggcacttaa agaagatgaa aaaggtaaaa
     7621 ccattttaaa aaattcatca aaaataaagt atgtagaagt tcctaaagaa atgttatttg
     7681 atatcgatac aaaaatggac tatgagagaa tcgggggtaa attatgaagc taataaaaac
     7741 tatagatgct gttggacaag ttttatgtca cgacataact caaataatac ctggagaatt
     7801 taaaggaaga aaatttaaaa agggtcacat agtaaaagaa gaagatattc cagtgttatt
     7861 atctcttggt aaagataatt tgtatgtatg ggaaaagtca gaaggaatgg ttcatgaaaa
     7921 tgaaggcgca ttgtttttaa aagaactaac tgctggagaa aatttagatt ttagtgaaat
     7981 aaaagaagga aaaatagatt ttatagcagc atgtgatggt ctattaaaga tagatgttga
     8041 tgcacttttt gatttgaatt gtgttggaga aataatgatg gctactcttc ataataattt
     8101 tatagtaaat aaaggtttaa aagtagctgg aacaagagtg atacctttgg ttatagatga
     8161 gaaaaaatta gaagatgcaa aaaaagtagt tggtaataga aaaatagtaa atgtagtacc
     8221 ttttaaacct aagaaagtag gaattgttac tacaggaaat gaagtgtttt atagcagaat
     8281 tgttgataaa tttggaccag taatagaaga aaaagtaaaa ggctttggtt gtgaagttat
     8341 aggacagact atatgcccag atgataaaga aattataaag agtgcaataa aagaatttat
     8401 aagtcaagga gcagaactta tctgttgtac tggcgggatg agtgtagacc cagatgatgt
     8461 tacgccaaca gctataaaag aaactggagc tgatttagtg acatatggtt caccaatatt
     8521 accaggagct atgtttttac ttgcttatta tggagaagtt ccaattatgg gtataccagg
     8581 atgcgcaatg tatcataaaa ctacagtatt tgatatagtt ttatctaggg ttttaattga
     8641 tgaaaagctt gataagtatg atatagctag atatggtcat ggtggccttt gtatgaactg
     8701 tgatgtatgt acttatcctg catgtaattt tgctaaaata taaagttatt aaaaaagagt
     8761 atttacaaat taagtactct ttttaaatat gaaacaaaac agctagaatg atgttttaag
     8821 aaaagcatat ttatttagaa attataataa cttattttca atgtgtaaaa gaatgaataa
     8881 agttgcaatt tgatttcttt ataagtataa tataagagat tgttaatttt tataaattta
     8941 caatttttag tattatttta ataaatatt
//
LOCUS       DAEPOG010000153         8904 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEPOG010000153 DAEPOG010000000
VERSION     DAEPOG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.153"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            153..1214
                     /locus_tag="KR432_002952"
     CDS             153..1214
                     /locus_tag="KR432_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH0641468.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1305..2918
                     /locus_tag="KR432_002953"
     CDS             1305..2918
                     /locus_tag="KR432_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0641469.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3193..5178
                     /locus_tag="KR432_002954"
     CDS             3193..5178
                     /locus_tag="KR432_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0641470.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            5183..5617
                     /locus_tag="KR432_002955"
     CDS             5183..5617
                     /locus_tag="KR432_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBH0641471.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            5646..6206
                     /gene="srlA"
                     /locus_tag="KR432_002956"
     CDS             5646..6206
                     /gene="srlA"
                     /locus_tag="KR432_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0641472.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            6270..6638
                     /locus_tag="KR432_002957"
     CDS             6270..6638
                     /locus_tag="KR432_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBH0641473.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            6663..7229
                     /locus_tag="KR432_002958"
     CDS             6663..7229
                     /locus_tag="KR432_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0641474.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            7270..7635
                     /locus_tag="KR432_002959"
     CDS             7270..7635
                     /locus_tag="KR432_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0641475.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            7736..8509
                     /gene="srlD"
                     /locus_tag="KR432_002960"
     CDS             7736..8509
                     /gene="srlD"
                     /locus_tag="KR432_002960"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBH0641476.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSNMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         3558 a          912 c         1646 g         2788 t
ORIGIN      
        1 aaaataaaaa attgtcgctt taaagtataa aacagattat atgttaataa tgtggtgaaa
       61 ttatacccat atataaataa aataaaacaa ataaatagct aattaaggag aaaatatttt
      121 ttattttttg tttaggataa ataaatttta ttatggaaaa aagaaaaagc atacatcttc
      181 acaaattgaa tatttattta atagggggag gtgatagatt gtttatcaca gtggtattat
      241 ttttagtagg tttcctatta attactaaag gcgcagatat ctttataaat tgcactgtag
      301 agataggtag aaaaacgaat atatcagaaa ttatacttgg agctactata gttagttttg
      361 caacaacatt acctgaattt acagtgtctc tacttgcttc tattgatggt catactacta
      421 tgagtttagg aaatgcagtt ggttctataa tatgtaatac aggattagca ttagggttgg
      481 ttgtttttat aagtccattt aatgtagata aaaaaatgtt tttttctaag tctttactat
      541 taatagtatc cgttatagtt ttaattttat tgagtttaga tggagttata acaagaggtg
      601 attcattatt acttataata atattaatct tttatatggt taataattat agaagtgtcg
      661 ttggaaaatc tgatactaaa aatagaaata ttaaaaataa cactattaaa gacatatcta
      721 ctaaaaaagg gaaaaattat agaggttttt ccgtattaga aattgtaaaa atactattac
      781 tttttgctac aggattgata atgatgataa taggttcaca aatccttata gagagtggag
      841 taataatagc tagtttttta aatatacctc agggtatagt aagtttaact ataatagcac
      901 ttggaacttc tctgccagag atagtttcgt cgattactgc tataagaaaa aatcatcatg
      961 aaatatcagt aggaaatatt ttgggtgcaa acatattgaa tatagtatca gtaatagctg
     1021 tatcagcaat accaaataac ataccaatac tatctcaaaa tagacaattg gatataccat
     1081 tcatgatatt attattatta atagtaataa taccaacact taaatctaat agacttagta
     1141 gaatacaagg tatattgatg ttattcacat attttctgta catatcaatt ttgtatttca
     1201 tgtatattat ttaactatac tgtttaaaat attaattgca gaatatccta aaaaagtata
     1261 taatggatag gttaagatta attgataacg gagaggaata atatatgaat ataacaaagt
     1321 ttgaagattt accaataagt gagggaataa aaaaagctat agctgaaatg gggtttgaag
     1381 aaccatctcc tatacaagca cagtctatac ctgcgatttt gtcaggtaaa gacgttatag
     1441 gacaagctca aactggtacg ggaaaaacag ctgcttttag tatccctata ctagaaacaa
     1501 tagacccaaa taatagaagt ttacaagctg tagtgctttg tccaacaaga gaactggcaa
     1561 tacaagtatc aacagaaata agaaagttag ctaagtattc gcacggaata aagacattac
     1621 ctatatatgg tggccagcca atagatagac aaataaaatc tctaaagagt ggtgtgcaag
     1681 ttgtaatagg tacaccagga cgtacaatag accatatcaa tcgtaaaaca ttaaagatgg
     1741 ataatgtaaa aatgattata ttagatgaag cagatgaaat gctagatatg ggatttagag
     1801 aagatataga aatgatttta agtaagattc cagaagaaag acaaacaact ttcttttcag
     1861 ctactatgcc aagaggaatt ttggaattaa ctaagagata tcaaaaagac ccagagcata
     1921 taaaagttgt tagaaaagaa cttacagttt caaatacaaa gcaatactat atagaaacta
     1981 ggtcatcaaa taaattagaa gtattatgta gattagtaga tgtatatgac cctaaactat
     2041 ctgttgtttt ctgtaatact aagagaaaag cagatgaatt ggtaggagat ttacaagcaa
     2101 gaggatattt tgcagatgca ttacatggtg acttaaaaca gactcaaaga gatatagtaa
     2161 tggataagtt tagaaatggt acaatagata tacttgttgc tactgatgtt gcagctagag
     2221 gtatagatgt tgatgatgtt gaatgtgtat tcaactatga cttaccacaa gatgaagagt
     2281 attacgttca tagaattggt agaactggtc gtgctggtag agaaggtatg tcatttactt
     2341 ttgtatttgg aaaagaaatg agaaagatga aagatataga aagatatact aaatctaaac
     2401 taataaagca taatatacca acaataactg atgttgaaga gaaaaaagta ggtactttct
     2461 ttgcacaagt taaacaaact atagaagaag gtcacttaac taaacaatta caatggttag
     2521 aaggtttctg taatgatgaa gattatgcaa tggttgatat agctgctgca cttgtaaaac
     2581 tatctctagg tgaagaaatg aaagaagaaa taatagaaga aaaacctaga agagagcgtg
     2641 gagatagaaa aggtggtact ggagctaaag atggtatgat tagattattt atcaatattg
     2701 gtagaaatca aagagtacaa gcaaaagata tagttggtgc tatagctgga gaagttggaa
     2761 taccaggtaa agtagtaggt actatagata tatatgataa gtatactttt gttgaaatac
     2821 ctaaaaaaga tgcaaaaaca gtaattgaaa agatgaaaga tattaagata aaaggtaata
     2881 agattaatat agaaaaagca aataagaaga aaaaataacg tatagtatac aaaactataa
     2941 ttagaaaata tataaaataa tatattggat atttaaaaag cctgatgtgt tttcaaaaca
     3001 gatagtagat aatctgtaag agttaacagc ataaggcttt tcttgtttta tatgatatta
     3061 tgattttcca gatcgttgtg aaaaatgtat attagttcct ttttttcaaa tctgatttga
     3121 aaaagagaat attaattatc tatatagttt agattataat aaaaaatgta agataacaaa
     3181 ggggagaaaa agatgtcaat gacttatatg gaccctaggg tttttttgat tttagaaatg
     3241 gtgttagaag aagacacaaa tactctagaa caattttcaa atgttttggg tgttagtact
     3301 aggactatca gaaactatat aaaccagatt aatcgagaat taaaaggtgg tattgctaaa
     3361 atagttaaaa atgaaaaagg tacatatagt ataagaattg aggatgaaag taaattaatt
     3421 gagattgtaa attttaatag aggaaagcta tcaaatttta taaatttaaa ttcacctgat
     3481 gaaagaataa attatgttct tgatatttta actatgacca acaatccaat taccatagat
     3541 gatttggctg aagagatatg tataggaaga acaacattaa taaaggattt aaaaaaagca
     3601 gacagaattt tagctgaata tgatttagaa ttaaaaagga agccaaatat aggtatgatt
     3661 ttagttggtg aagaaatgga tataagatta ttaatccttg atagattgta tgaaaattat
     3721 attgatgtct tggaaaacgt taatcagata aatttagtta aagattatga tattgaatgt
     3781 cttagagatg aattaaaaaa gttgtttaaa aaagaagaac tctacataac agaacaggtc
     3841 ttgagagatg ttgaaagata tataatcatg tctgtcttaa gaaatctaaa tgggtacaaa
     3901 tttgaaaaga tagataagag atttgaagtg ataagatgtt cagatgaata tactttagga
     3961 ttaaagctca aagctttatt ggaaaagata tttaatatag ttttaaatga aaaagaaacc
     4021 atatttttga caatgccatt aataggtaga aaagctccta gtacaaaatt agcacttagc
     4081 agtataaaga taaatgatag cgtaaaggaa gttgtagatg aagtaactag ttttttatta
     4141 gagacctcag ggattgattt taaagaagat aaaaagctta tagaaaattt ggaatatcac
     4201 ttgtattttg cacttaatag aatgagattc aacataaggg taaaaaatcc tcttttacaa
     4261 gaaataaagg aaagatatac atttccatat agtgttgcaa agatagctgc aatgataatt
     4321 gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag gatatatagc attacatttt
     4381 ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg taaatagggt agctgttgtt
     4441 tgtggaacag gtcttgggac tgcacaactt attagaataa agataggaaa aatacttgga
     4501 gaaagtgtag aagtaaatac tttttctgat atagaagcta aagctaattt agatttatta
     4561 aagtattatg atttagtatt tacaacagta gatttcaaaa cagacttaga tgttccaatt
     4621 tttagagtaa atgcgttatt taatgaagaa agtataaaga aagagataga aaatgcaata
     4681 cttttgaaaa atagtataaa tcttaagtct ataaaaaaga ttgaaaaaaa tatccctttt
     4741 ataggtaaat taattgaaga ggataagttc tttatattga ataagagtac gtttatggaa
     4801 aatttagaag atatgttaga aaaattagtt cataaaggaa ttattgatat agagttcaaa
     4861 aaaaaggtat taaaaagaga aaagaaatct tcaacagcat tagataatta tatagcattg
     4921 cctcactcag ttaatactaa tggggaaaat ctttatctgt catttggaat attagaaaaa
     4981 ccagtggttt gggatacaaa ggaaattaga ataataatat tgatgataat tccagataag
     5041 aatgtagata gtacagacct gataataaaa agttatgaag aagtacttga agtagggaga
     5101 gataaaaaaa tgatagaaga acttgctaaa gtaaaatcat ttgatgaatt ttataaaata
     5161 attaccaaga ggaggtaata atatggaccc agtaattttt attatagcat cacttattac
     5221 agtgtattta ttaaatattt ttcttggata cttacagctt aaagatttta ataaaaatta
     5281 tattgaacta aaaagaaaag gaagagtggc aataggcaga aaaaaaggaa gaatttcatc
     5341 aggtacaatt gtgttgatac ttataaatga tgatggtgtg attgtcgaaa ctagaaaaat
     5401 gcaaggtgtt acagtattgg caagagtaaa gtcatttgaa ggtttggtag gaaagagctt
     5461 agagtacata gaaaaatcag atttaactgt ttacaataaa ctacttaaaa aagcaatact
     5521 agatgcagtc aaacagtaca taacatttga gaaaaataaa tgtgaagata caaataatct
     5581 gagtactgat gaagagtgta ggatggaagt agtttaaaaa aatatattta taaggaggag
     5641 ttaatatgac gcaatttttt caagtattag caagtggtgc agatagtttt atgaatttat
     5701 ttagggcagg tgggcaacaa tttgtaggtt ttgtaacaga tatagtacca cttttagtag
     5761 cattattagt tacaatgaat gcaattatca actttattgg tacagataga gtcgagagac
     5821 ttgcaaaaaa atgttctgca aatatattta ctagatattt gatattacca gtccttggta
     5881 catttgtatt tgcaaaccca atgacactat cactaggaag attcttgcca gaaaagttta
     5941 aacctagtta ctttgcagca gcaagctttt catgccatac aatgaatgga ttattcccac
     6001 atatcaatcc aggtgaacta tttatatatc taggaattgc aaatggtata acaactttgg
     6061 gattctcaac agcagattta gcagttagat atttgttagt gggtatagtt gcaaacttta
     6121 ttaaaggtgt agttactgac tatacaacaa aatttgtaga gaaacaacaa ggaatagcat
     6181 taaattcaga tataaagatt ggataattac tttaaggtat aaataaattt ttcaaataaa
     6241 aagatagtgt atagaggagg ataaattata tggcatttaa caaagttaaa atagtaaaag
     6301 gttctggagg atggggtgga ccattgataa tagaaccaac agaaaagaag aataaggtag
     6361 tatatgtgac tgggggagca aagccagaaa ctgcagtaaa aatagctgaa cttacaggat
     6421 gtgagttaat agatggattt tcacagggtg ttaaagatga tgaaatcgca tgtgtaataa
     6481 taaattgtgg tggaacattg agatgtggta tatatcctca aaagaaaata ccgactataa
     6541 atataatgaa aacaggaaaa agtggaccat tagcaatgtt tataaaagaa gatatatatg
     6601 tttcaggggt aaaaccaaca aatgtagagc agttataatt gaggtagtag gagggaagaa
     6661 atatggctta tgatactaaa aagaagataa gtgagcagag tcaaggaatt gttgcaaaaa
     6721 taggtatagc actaggaaat gtgacaagta ctctttttca agctggtagg gatactatag
     6781 atacattaat acatactata ctgcctttta tggctttcgt tgcagtccta ataggtataa
     6841 taaatcaatc aggtatagga aattgggtag ccaatttact tactccatta atcggaaatg
     6901 ttggaggttt agttgtctta tcaataatat gttcatttcc gttgttgtct ccgtttcttg
     6961 gaccaggagc tgttatagct cagatagtgg gggtattggt tggtacagaa ataggaaaag
     7021 gaaatattcc accaagttta gcattaccag cattgtttgc aataaactca caagcagctt
     7081 gtgactttat accagtaggt cttggtcttg cagaagctga acctgataca gttcaagtag
     7141 gagttccgtc agttttatat tctagatttt taacaggtgc accaactgtt ctattagcat
     7201 ggttagcaag ctttgggtta tataaataga agaatacttt ataaataatt agagagaggt
     7261 tcagttgata tggaattaat atacagtact caaataaata aaataggaga taatgcaaat
     7321 gaatttttac aacataatat gtttgtaact ttcaaagata atgctcctca agagttagaa
     7381 aattactgtt atatacattc tgaaaataat ttagtaaaag aaatacttac gaatgatgtg
     7441 ttatcaataa ataatgttga ttacaaaatt acttctgttg gtgagttagt aaaccaaaat
     7501 ctttcagaat taggtcatat aacatttaag tttacaggtg aaaaagatgg agcaattgga
     7561 gggaccttat atcttgaaga aaaagaaata gcacctattg gcgtaggtac tatactaaaa
     7621 ataattagaa aataaaattt aaaacgaatc taatcatatt ataagtaaat tatagataaa
     7681 agtcaattta actaatcaag ttaactttta atgtgataaa aaaggggaaa tgaaaatgag
     7741 aaaagtggct ttagttgttg gaggaggaag aagtttaggt gaatatttat ctaaacattt
     7801 agctaaagta ggttatgatg ttgctattgc agatttaaat gaagacaacg caaaaaaagt
     7861 agctgaagaa atagaaactg aatttggttg taaatctgta ggatatggtt gtaatgccat
     7921 agatgaaaga caggttataa gcactgttaa agctattgaa aatgattttg atagaataga
     7981 tttattagtg tacaatgcag gagtggctgt aagtaaaaaa ataactgatt ttggatacaa
     8041 agattttaaa tttattgtag acgtaaattt aaatgggtac tttctatttg caagagaagt
     8101 atctaaagtc atgatagcaa aaggtattga gggtagaatc atacagataa attcaaaatc
     8161 aggtagagtg ggaagcaagc ataattcagg atattcagca ggtaaatttg ctggagtagg
     8221 acttactcaa agtttagcgc tagatttggc agaacataag ataactgtaa atgcactgat
     8281 gctcggtaat ttacttgatt ctaatatgtt tgaaagttta attcctcaat atgcagtaaa
     8341 attgggaata cctgaatcag aagtcaagca agtttatata gataaagtac ctttaaaaag
     8401 agggtgtaga tttatagatg ttgcaaatat gcttacattt tatgcttcaa aggaagctga
     8461 atactgtact ggtcaatcta taaatattac tggtggacaa gttatgtaag gtttttatgg
     8521 agtgtctcaa gatgaactct ttagttcatg aggcactttt tttgtataaa attaaatata
     8581 ttttcatcat ttaaacaatg aaaaagaggt ttatctttat tttgagatag cctcattttt
     8641 gtaattaaga aacatagtgt ttaagtaaac atataagatt gttagttttt taaaagagaa
     8701 ttttttaata atattatatt tatgaagtat ttttaaactt aatttgatta aaaaatagct
     8761 acgcacactt gtgactttag tcatgagtta gtagctaaat tagtcagcat atagggaaac
     8821 ttatatgtag agataggata gaacctatcc aatactactt gaattgctaa aaacccctaa
     8881 agctaaccaa actaaaacat aagg
//
LOCUS       DAEPOG010000154         8867 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEPOG010000154 DAEPOG010000000
VERSION     DAEPOG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.154"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(86..997)
                     /locus_tag="KR432_002961"
     CDS             complement(86..997)
                     /locus_tag="KR432_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0641477.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1922..2773
                     /locus_tag="KR432_002962"
     CDS             1922..2773
                     /locus_tag="KR432_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0641478.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            2777..3247
                     /locus_tag="KR432_002963"
     CDS             2777..3247
                     /locus_tag="KR432_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641479.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            3260..4570
                     /locus_tag="KR432_002964"
     CDS             3260..4570
                     /locus_tag="KR432_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641480.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            4782..5528
                     /locus_tag="KR432_002965"
     CDS             4782..5528
                     /locus_tag="KR432_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0641481.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            5890..7431
                     /locus_tag="KR432_002966"
     CDS             5890..7431
                     /locus_tag="KR432_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0641482.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            7466..8572
                     /locus_tag="KR432_002967"
     CDS             7466..8572
                     /locus_tag="KR432_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0641483.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         3079 a         1236 c         1328 g         3224 t
ORIGIN      
        1 ttttaatata attaaactca gtgagatatt atatactaaa tgcaagatat tatatattta
       61 ataaaatctt taatctagca tcatattata atgaataaat tttagacaac tcccttagtc
      121 tagttattac attatcccta acatctttag gttcaagtac ttcaacattg tcaccaaacg
      181 ataaaatata tccataaatc cattcaccaa taggaaattc tgtaattaca tcatatgtat
      241 catcttcatt tttagttatt aaatccttat taaaatcatc aaatactcta aaaagcatct
      301 tttctttaaa cctcaatttc aacgttatag tattttcctt aattacttta gaatcattca
      361 aacatacttc ttctattatc tttcttgaaa atacttcatc ctttatagat aaattcctta
      421 tcctactaat tctaaatatc ctaaaatcat cttttagttt acaaaatcca tacaagtacc
      481 aagtcttact cttatacata agcttcagtg gttcgacagt tctgtttgtc tggcttgaca
      541 aactatttac ataattaaaa tttatagtat tacgtcttaa aatagcttct tttatttttg
      601 taaatttatc atcttcatta aaattacttc cccattcagt aaagtctatt tcaatccagt
      661 ttgaaaaatt ctgctctcca aatattgagc caattttatt tactactgaa tttatttctg
      721 gatacttagt cgcctgtaat gttttcagtg caactattaa actctcttta tctttttttg
      781 acagtatggc cttgtctata gaatattctt ctattaaaga tatccctcct cctttgcctt
      841 tcgtcatata tactggtact cccgacatag acagagtttc tatatctcta tatatggttc
      901 ttgtagaaac ttcaaaccta tctgccaact ctttagctgt tactaatttt ttatttaata
      961 gaattactat tatttctgtt aatctattta ttttcattca ttaccaccta ctaaaattat
     1021 taaatttatt taatctaaat taatcatact aatttttaaa taaataaaat cttagaatct
     1081 tatctaaatt attcttacat gattttattt actttctatt atctttctca ctttggatat
     1141 tatcatctta atttatttat tactatatca atttacttat tatcatctca tttgttatca
     1201 tcttacttaa tttacttatt tctatatcaa ttcacttatt atcatctcat ttgttatcgt
     1261 cttacttaat ttacttattg ctatatcaat tcacttatta tcatttcatt tgttatcgtc
     1321 ttacttaatt tacttattgc tatatcaatt cacttattat catttcattt gttatcatct
     1381 tacttaattt acttatttct atatcaattc acttattatc atcttaattc atttgcttat
     1441 tatcatttac tttattcaat ttctatatca aaaattagta tttgtactta atacttaaaa
     1501 ttaatttaaa gtctttacat attatttatt ttctattgta atattcattt ccatacttta
     1561 ctaagctact tccactcatt tattttaaaa tttcaattta ctatttctca actctatatt
     1621 attatttttc aattttatat tactaatttt aaagcttttt agctattatt cctacaatat
     1681 ttaaacaatt ttttcataat aaagctatat tacaatatat tcaaaatatg taatatttta
     1741 gatatttttc gttgacaatg ttaaattttt gttttatcct taattaagga catgttattg
     1801 acatttctga aacttatttg aaatagtttt ttcattttat ttaggaaaac gtattatact
     1861 taagaaaacg tattttctca tttcagataa gtaaaatatt tattcttttg gggaggaaat
     1921 catggaaatt aataaaaaac attttattca agtattttta acttgcttaa tcttggtagt
     1981 aatatgtgaa tttatagggc aaagaactat agctattgga aaagcctcta tagtactgtt
     2041 ccctatgctt tatgcagtta taattggact tataataaca ccagatttat taggtaaaaa
     2101 aattaaagca cttaaaaaag tggttggaga aaaagaaata aatattgctg gtgaagttgt
     2161 tggaattgca ctagtaatgc ttggtataaa atatggtact acagttggac caaacattga
     2221 caaaataatt caagctggac cagcatttat cgctcaagaa tttggtcata tacttgcacc
     2281 aatcgttgca ttacctcttg cattaatgtt tggaatgaaa agagaagctg ttggagctac
     2341 tgcaagtata agtcgtgaac cttctttagg agttatatca gaaaaatatg ggataaactc
     2401 accagaaggt agtggtgtac ttggtacata tttaatggga acagttcttg gtactatata
     2461 ctttagtgta ctaggttcaa tatctattta tagtggtctt cacccatatg ctttgggaat
     2521 ggcctgtggt gttggtagtg gaagtatgat gacagcagct gctggttcat tatctaccat
     2581 ggtaccacct gaaatggcag atacaatact tgcctatgct gcaacaagca atatgatgtc
     2641 tagtataaca ggaattatat tcttagtttt tgtatcctta ccatttacta attttatgta
     2701 taagatttta gaacctaaat tttcgagaaa taaaaaaact aaagaaatta aatctacaga
     2761 aggagagtgc taataaatgt ttgctaattt aaatttaaag aaaagttcat atcaattatt
     2821 ggttatgtct gttattggag ttataattct tataggacaa agaattagcg ttggtacatc
     2881 tatagttaca gctcttcctg gtatggtgat gctaatatta gctgctatgg cagctatgat
     2941 tataaaagat ttatttccta aatcaatctt tccagctttt ggatttgcaa ctataatcgg
     3001 ccttttactt agtatgccat atagtccaac atctgaagta ttcttaacta acactaataa
     3061 tattaacttt atggctataa ctactccact actagctttt gcaggaattt ctgttggaaa
     3121 caaaatagaa gctttaaaag aaatgtcatg gaaaatagtt ataatttcac ttattgtatt
     3181 tacaactata ttctttgcat gtgcgagtat agctcatata gttttaagta ttcaaggtaa
     3241 aatataggag gaatttatta tgaataaaga acaattaaaa tctctagtaa taaaaactat
     3301 agatgaaaat agagaaaaaa tattaaaaat aggtcaagga atatacaaaa atccagaata
     3361 tggatataaa gaatttaaaa ctacagaagc tgtttcaaac ttttttgagc aagaattaaa
     3421 tttatctgta gaaaaaaata tagcagttac aggatgcaaa gcagatgcaa attctagcaa
     3481 gaaaggtcct catatatcta tacttggaga attggatggt atatcatgta aagaacataa
     3541 agatgctaat gaaataggtg cttctcatac ttgtggacat aatattcaaa tagctggaat
     3601 gttaggtgct gctgttggtt tagtaaaaag tggcgtttta gaacatttag atggtaaagt
     3661 atcttttatg gctactcctg ctgaagagtt tattgagttg gaatacagag agcaattaaa
     3721 aaaagaagga aaaataactt attttggtgg aaaacaagaa ttaataaaaa gaggttattt
     3781 tgatgatata gacatatcta tgatgtttca tgcttctgat ttaggagaag ataaggctct
     3841 agttggtcct gagagtaatg gatttgttgg aaaaaaggtt aaatttatag gtaaagaatc
     3901 tcatgctggt tctgctccac atgatggaat aaatgcttta aatgcagcaa tgcttgctat
     3961 aaacaacgtg aatgcactta gagaaacttt taaagagtct gaaagagtta gattccaccc
     4021 tatcatcaca aaaggtggag atatagtaaa tgttgttcct gctgatgttc atatggaatc
     4081 atatgttaga gctagaacta taaatggtat gattgatgct aatgaaagaa taaataaggc
     4141 tttgatggct ggtggaatgg cagttggagc tgatgttgag ataactgaaa ttcctggata
     4201 tcttccaata ttacgataca aaagtcttga taatttattt aaaaacaatc ttgaagattt
     4261 aggtctaaaa ggaaaagtta ttgatggtgg agattttaca ggctcttttg attttggaga
     4321 tgtgtctcat ataatgccta ctcttcaccc tatgattggt ggtgtaaaag gagctcttca
     4381 tacaagagat tttgaaatag ttgatgaaga tttagcttac ataattcctg ctaaagcaat
     4441 ggcacttaca gttgtagacc ttctttttga taatgctaag gatgctaaag atatattaga
     4501 tacctttact ccagttatga ctaaagaaga atatctaaca ttcctagaaa aacacgataa
     4561 aactttataa gtctattttt ataatagggt ggtatctact gtaaaagtta taccactctt
     4621 tgtttttgta acagtatact tcttttcttt tagcttttag attaaactaa aatatattat
     4681 cctccttaaa acttttagag atacgttaaa atatcagtag aactatgcta tattctttct
     4741 aattaatata aatttatatt ctaattcaag gtggtgaaaa aatgtttaat gcaggaaatc
     4801 gaattataaa tacatggttg taccctattc aagatggctt tgtcttgatt gatactggat
     4861 atgaaaatgg ttttaaacat ttcaaaaaac gaatttctaa attcaacata aaaatcaaag
     4921 atattcgata catatttcta actcatgctc atgatgacca tgttggtttt ctaaatcaga
     4981 ttctagacga atctcctaat acaaagattg ttatgcatga taaagcttta gaaattctat
     5041 ataaaggtca aaactctttt attggtggtt gtaacagccg tactgccttt ctcttttgcc
     5101 aaattatgaa actatttgga aaaggtgagc atagtttttc tccacttaaa caagaatttg
     5161 aaaaaaggtg tattcttgtg tctgacaaga atcgtaaaga aattgaaaaa gaactaaatg
     5221 gaaaaattat agatacacca ggacatactg ctgattcaat ttcactacta attgatgatg
     5281 gtgttttatt ttgtggagat tctgcaatga atggatttcc aagtactcac aaaattacca
     5341 tatttgcaga aagcaaacta gaatttattc aatcttggaa aacaatcatc aatacaaaac
     5401 ctaaaatgat ttatcctgga catggaaaac catttgaata cagtaaatta gaacaaaatc
     5461 taatctatgt aaataaaata aatttatatc ctctacataa ttcaaaagat attcacagat
     5521 ttttgtagaa aatacaaagt aaatttcttt gattaattaa aaaatctaac actttactac
     5581 gctacttatt ttaataactt aatcttccac tagcttttca taaaattttg ttttaaaatt
     5641 aactttaaag tgcatatgct atttaaatac attagatata attcgtcatt aatctactta
     5701 aagcatattt aactaacacc actcaaccag aaaattcaga aaactgcatt gacacaggaa
     5761 aaagtttatt ttatactttt tttaggacat attgtggaca cgtcttaaat cgacaagcag
     5821 aatttatatt gtaagatcaa ccacaatact tattttttaa ttatattttt aaacattagg
     5881 gaggatatta tgaatgataa cgtagttaag aagaaaaact tttttgacag gacattaaac
     5941 agaatagaaa ctgttggtaa taaactacca gacccagtta caatattttt agggttatgt
     6001 gtacttttac ttatactatc tagtctagtt ggttctatgg ggatatcagt agttcatcct
     6061 ggtactggag aaactataac tgcagtaaat ttattgactg tagaacagct tcaaattctt
     6121 ttaggaaaca tagttagcaa tttccaaggg tttgctcctt tgggtctagt tctagtaact
     6181 atgataggtg ctggtgtatg tgacaaaaca ggacttatga cagcaacaat caaagcatca
     6241 gtaagcaaga ttcctgaaac aagagtaact cttgttgtaa tgactatagg tatgcttgcc
     6301 aatatagctt cagatgcagg tacaatatta tttccaccat tagctgcact tgtatatctt
     6361 ggtgttggta gacatccact tataggttta ttttctggat atgcagcagt ctgtctagga
     6421 tttgccgcaa acattatgat aagtgtcaat gatatacttg ctgcaagttt tacagttcca
     6481 gcagctcaaa tgattgattc tggatttcaa gccaatgcaa caatgaactt attttttatg
     6541 atagcatcaa cttttgttct aatacttttg gcaacatggg taacagaaaa aattgtagct
     6601 cctagatttg gtaaatatga aggaaatgca gaactagatg ttgatggaag tcttagtgat
     6661 gtagaaaaga aagggcttaa aaaagctggt ataagtatat taatatatgt agctattata
     6721 gtagctctta gtgtaatcgg taaaaaacca ttcttagctg acccagaaac aggagattta
     6781 ctatcaaaca atgctcctct tatgaaaggt atggtaccta taatagcact agcatttttc
     6841 atacctggtg ttgtatacgg aaaaactata ggtaaaataa agaaggataa ggatgtagtc
     6901 tctataatgg ctagtgcaat gagtgatatg ggtggatata taatactagc atttgctgct
     6961 tcacaatttt tgcaattatt cacaaatagt aatttaggtc tgatactagc tgtaaagggc
     7021 ggagaatttt taaaatcagc aggaataaat ggacctttat tgttgatagg atttattata
     7081 ttatcatgtt ttataaattt atttataggt agtgcatctg caaagtgggc tattcttgca
     7141 cctatatttg taccaatgtt tatgatggtt ggatacaatc cagcacttac tcagatggct
     7201 tatcgtatag gtgattgtat aacaaatcca cttagcccat tatttccgta cttccctatt
     7261 atactagcat tcacaagaaa atacgataaa gatgctggta tgggtactgt aattgctaat
     7321 atgataccat actctatatc attcttaata gtgtggacta tactattaat attgtttatg
     7381 gtatttaata ttccactagg acctggaata cttccttcat atagcatgta atttatttaa
     7441 atctttctaa aaggagaaat aatttatgtt agataaaaat agacttataa ataattttat
     7501 ggatatggtt agaattgata gcccatctaa tcaagaatta gaaatgtcta agtggttggt
     7561 aaactactta aaagaaagaa atatagatgc cattatagat gatgcaggtg aaaaatatgg
     7621 tggtaataca ggtaatgtca ttgcctatat aaaaggtgaa gaaggaagta gaccactttg
     7681 tttatgtgca cacatggacc aagttcaacc ttgtttaggt gtaaaaccaa tactagatgg
     7741 taatgtagtt agaagtgatg gaactactac acttggtgca gatgataaag ctggaatagc
     7801 tgctatactt gaagctttag aacatgttac tacagaaaaa ataccacata gagatattta
     7861 tctttgtttt acaatctgtg aggaagctgg tatgcatggt gtcaaaaact ttaatcctga
     7921 caacttgcct tgtaaagata tggtgatttt agattctggt ggagctattg gttcgatagc
     7981 atacaaagct cctgctcaac aatctataaa aatctctttt catggaaaga aagcacatgc
     8041 tggtatagaa cctgaaaaag ggttaaatgc tatattagta gcctctcatg caataagcaa
     8101 tatgcacata ggcagaatag atccattgac tacttcaaat ataggtaaaa tagaaggtgg
     8161 tggagctaca aacatagtaa ctgataaggt tacattgact gctgaaatta ggtctcatat
     8221 acctgaaact ttagagtatg agcttaatca tatggaaaaa tgttgtaagg atgctgcatc
     8281 taaatttaat actacttata cctttgaaca tagtatgtct taccctagtt ttgaacttag
     8341 tagagatagt cacgttttca agttaagtga agaagcaata agacaagttg gtgttgttcc
     8401 aaatccaatg gtaattggtg gtggaagtga cgcaaatata ttagctaatc ttggatataa
     8461 ttgtgctata ttaagtctag gtatgtatga tgtacacaca gttaatgaat atgtaaatat
     8521 agacgaatta tatgatactg ccaaaatagt ttatcatatg attaaattat aaatatcata
     8581 atatacctct ttaaattata aaaataaaaa cctatgtatc ttcaacttta taagttatat
     8641 attaaagttg ttttacatag gttttagtat cttatataat aatttatttt aacatttttc
     8701 ataataagct ttggtatttt atatagtaat ttgttctaac atttttcata ataagcttca
     8761 atatttttca taataatttg ttctagcatt tttaataaca agttctagta tttttataat
     8821 aatttgttct agtattttta ataacaagct ttagtatttt acataat
//
LOCUS       DAEPOG010000155         8791 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEPOG010000155 DAEPOG010000000
VERSION     DAEPOG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.155"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..642
                     /locus_tag="KR432_002968"
     CDS             <1..642
                     /locus_tag="KR432_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH0641484.1"
                     /translation="SCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFD
                     KSISCIEYNDVTKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKR
                     LNKRGFNQAQKIAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKEN
                     VKLINNKNILLIDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            974..2887
                     /locus_tag="KR432_002969"
     CDS             974..2887
                     /locus_tag="KR432_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0641485.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            3028..3336
                     /locus_tag="KR432_002970"
     CDS             3028..3336
                     /locus_tag="KR432_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0641486.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            3361..3687
                     /locus_tag="KR432_002971"
     CDS             3361..3687
                     /locus_tag="KR432_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641487.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            3726..5060
                     /gene="celB"
                     /locus_tag="KR432_002972"
     CDS             3726..5060
                     /gene="celB"
                     /locus_tag="KR432_002972"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBH0641488.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            5071..5589
                     /locus_tag="KR432_002973"
     CDS             5071..5589
                     /locus_tag="KR432_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBH0641489.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            5685..6644
                     /locus_tag="KR432_002974"
     CDS             5685..6644
                     /locus_tag="KR432_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBH0641490.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            6692..7744
                     /locus_tag="KR432_002975"
     CDS             6692..7744
                     /locus_tag="KR432_002975"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBH0641491.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            7747..8421
                     /locus_tag="KR432_002976"
     CDS             7747..8421
                     /locus_tag="KR432_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH0641492.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
BASE COUNT         3606 a          868 c         1607 g         2710 t
ORIGIN      
        1 agttgtttta aagaaatgaa ttttatacaa gatggttgca tgaagtgtgg aaaaccaata
       61 atacgtcatt caattgaaaa agaatttatt gaagagtgta gttattgttt taataaagac
      121 ttttattttg ataaatcaat atcttgtatt gaatataatg atgtgacaaa aaagatggtg
      181 ttagggctga agtataacca aaaaaccttt atggctaagt atatagctca aataatgaaa
      241 gaaaaattat atttagaaaa cataaaattt gattatatat tatttgttcc acttcataaa
      301 aaaaggctaa ataaaagagg ttttaatcag gcacaaaaaa tagcatttaa tttaagtaaa
      361 atagtaaata ttccattatt agattgcata agtagaaaaa aatatacaag aatgctatat
      421 aaattgaata agaaagaacg aaaagaagaa ttaaaaaatg tatttgttgt taaagaaaat
      481 gttaaattaa taaataataa aaatatactt ttaatagatg acatatttac tactggtcta
      541 acaacaaacg aaatctctaa acttttaaag ttatcaggtg ctaataaggt ttttgtattg
      601 actctactta caaaagcaaa tgataattat attatggaat aatcttatat attgacaatt
      661 ttagtttaaa atattaaaat gaataagtga tatttaggtc tgtggttgaa agtccaagcc
      721 agtcgcaggc aaagggatcc acgtaagtta acactaaaaa ttgggttaat gatcatggtg
      781 cggcttagaa gtaagaccta ccgataataa aatcgagaga gagtagtagt gcggcgaacc
      841 caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta aatatcatat
      901 taaaagagag ctagcatacc tttatgtata gctctttttt atatataata aatttaggtt
      961 gagtcataaa gaggtgatat gtttgaataa aagacaagaa aatttattaa caacattgct
     1021 attaaaagaa gagtttataa atgttaataa gattgccaat gattttgagt gttcagaaag
     1081 gacaataagg aatgattgca aatatattga tgaatggtta tctacttttt gtaatgctta
     1141 tatcaaaaga aaacctaata ttggaattaa atttagtgga gatggaacag acaaaatact
     1201 ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat agtgagcttc ataagcaagt
     1261 tgagatatta aatttactac tttgtaataa taaaaaagtt actttaaatg atttatctaa
     1321 taagttgtac ataaataaaa atattgtcag agaagaaata agtaaattat cagtagcatt
     1381 aaataattat aatttaaatg tctcgactaa aaaaggcagt ggtatatata ttgaagggga
     1441 tgagaaagac ataagaataa tgttagtatc attcttattt aaatacatag ataaatacag
     1501 attaaacata gatgaaatag agtacttcca tatagtagat ataataattg taaaaaacat
     1561 aatgcttact atagaggagg atatggatat aagacttaca gatatatcat tcaagcaatt
     1621 gatgctattt ttgcttataa atataatacg aataaggctg ggaaattctt taaaagtatc
     1681 aaatactaag aaagagctta agctaaaaat gcttatattg gatatagaag atgaattgaa
     1741 atctaattta tccatagtat taaattatga agaaaaattg tttatagaat ctttaatatt
     1801 gggtatgaac aaacaaataa atagtaataa tattaagaaa tctttatatc taaatgaaga
     1861 attagcaagt tatacaaaaa aaattataaa tttagtatca gaagaatcag gaataaattt
     1921 taataatgat atattacttt atgaacaatt aatatgtcat ttgaatgtta caatgcatca
     1981 aatgaaaagc aatgtttact tagaaaatcc acttttagat aacataaaaa ctaaattttg
     2041 ttttttattt acagtaatat caagttcaat agaaattgaa ttaaaaggaa aacaaataac
     2101 ggaagatgaa attggttatc ttacacttca ctttcaaacc tctctagaaa gaaaatacag
     2161 caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca tttagttttg gagtaagtat
     2221 gctattaaaa gtaaagatag aaaagagatt tgataacata aaaattattg aaacattaag
     2281 agaggaagat ttaaaaagag ataactttaa taagactata gattttataa ttgcatttca
     2341 agagtatgaa aatatccaaa aaccaatttt tataacaaca ccattattta ctgatgagga
     2401 tgagaataaa cttaaagagt ttacaaacaa aataaaagaa aaagacacct catataaact
     2461 tatgaacaga ttaatgatgt ctgattattt aataagagag ttagaacatg atgatattta
     2521 tgaagctata acatatttaa ctaatttatt aattgaaaaa cattatgcag aaaaagagta
     2581 tttacaatct attataacaa gagaaaagtc atatccaacc aatgttggaa aaggaatatt
     2641 attccctcat ggagacatga aatatataaa acaatctgtt ttatgttttg caaggttaaa
     2701 aacaccaata aaaattagaa atggaaagga tataaagatt atacttttat tagcatataa
     2761 aaaagatgat gatagaaaag tatttagaga attatttaaa gaaatatcta atttaacaga
     2821 ggatgaaaat atgctagata ttctaagtaa atgtgacatt gaaaaggtaa aagatattct
     2881 tatataaaat tcataatgaa ctatggataa gcacaaaatt ttccatgtga tggaaaaagt
     2941 tgtgcatgtt tttttgtttt tataagatat tatatgtttg taaggtataa ataatctaaa
     3001 agtagagaag caatttagga ggaaaaaatg gaagaaatag ttttaaagat tataattcat
     3061 gctggaaatg caaaatctat gctttatgaa gcacttgact atgcaaagga aaacgatttt
     3121 aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc acataaagta
     3181 caaactgaac ttatacaaaa ggaagctggg ggagataaat cagatatatc tattttattg
     3241 attcactctc aagaccattt aatgacttgt atgagtgaga gaaaccttat aaaagaaatg
     3301 attatgttaa gaaaagaaat tcaaaaatta aaataaaaaa attaaggggg atttaaaaga
     3361 atgaaaagaa aagtatattt attttgtagc tttggtatgt caacaagttt acttgcagat
     3421 aaaatgcaga aggtagcaga tgaacacaat ttaccaatag aagtagaggc atttccgata
     3481 gcagaaattg acaaaatagt tgaagaaaaa aatccagatt gtatattatt aggaccacaa
     3541 gttaaataca tgctaaagga gttaaaacca aagtttgaag ctcaaggaaa acttatagat
     3601 ataatcaatg aagttgatta tggaactatg aatggggaaa aagtattaaa acttgctata
     3661 aagctaataa aatctaaaca agcgtagaat tatagtaaaa aataatagga aatgagggtg
     3721 gatagatgaa tagattggaa agaatactta tgccattggc tgaaaaaata ggtaaaaata
     3781 aattattaat tgcaattaga gatggttttt taatatcatc accattatta ataataggtt
     3841 cattattttt attagtagca aattttccta taaaaggatg gaatgaattt gtaggtcaaa
     3901 tttttggtcc agattgggct gtaaaattgc aacaaccaac tgtagcaaca tttgaagtaa
     3961 tgactttact tgcagtttta ggaattggat attcttatgc aaagcaaata gatgtagatc
     4021 caatagcatc agctgcagtt gcaatagttg ccttttttat agtaacaccg tttgtaatac
     4081 catatacacc agaaggaaca gaatcagtct acatggtaac aggtatacca ctaggttgga
     4141 tgggctctaa gggaatgttt gtaggtatga taacagcaat aacctctgta aaattatttg
     4201 ctgcagttgt aaaaaaagga tggacattga aaatgccaga tggagttcct ccaaccgtat
     4261 caaaatcatt tgcagcatta ataccaagtg caatagttat ggtagttttc tttttagtta
     4321 agatagcttt tgaagctaca ccatatggaa gtatacatga atttatattt aagtttttac
     4381 aaatgccatt attaaaatta ggaaatagtt taggtgctat ggcaatcgct tatatattct
     4441 tacatttatt ctggttcttt ggaataaatg gaagtagtgt tgttggggct gtgtataatc
     4501 caatattaaa aattctatca gctgaaaact tagctgcttt tcaggctggt tcaaaaatac
     4561 caaacataat aacaggtcaa ttccaagata tgtttgcgac atttggtgga gcaggtagta
     4621 cactatcatt agtcatagct atgttattaa tatgtaaaag taaacgtata aagtcaatgg
     4681 gaaaactatc aattttacca ggaatatttg gtataaatga gccattgata tttggtctac
     4741 caataatgct taacccaata ctattgatac catttgcaat agttccaaca ataaatataa
     4801 taatagctta cttctgtatg tcagcaggat tagtgcctct tacaaatgga gttcagttac
     4861 cgtggacaac tccaattata ttctctggtt ttttaacaac aggatggcaa ggtgcagtct
     4921 tacaattagt gcttcttata ctaggaatat ttatgtatat accatttata aaaatgctag
     4981 ataagcaata cttgagagaa gaaaatgaaa ctgttgaaga ggaagatgat gatatatctt
     5041 ttgatgattt agtattatag gaggaagatt atgaagaaaa tagttatgat attggatcaa
     5101 atacaagctg gagctggagg aaaagagaaa agtaatatac ctcctgctgg aaaatcaagc
     5161 ccattaggac caggtgtaat gatggagcaa tttttaaatg aatctaaggt aatagctacc
     5221 ttattttgtg gggatgaatt ttttgtaaat aatgaaaaag aagtaacgag taagatgata
     5281 gctatggtaa aaaaattaaa ccctgatgta gtaatctgtg gaccaagctt taattacgaa
     5341 aacttttcta agatgtcagc aatactgtct aaaaatataa atgataaaac agatatacca
     5401 gcttttgcag ctatgtcaga agaaaatata gatgtgataa acgaatataa aaatgacata
     5461 tgtatagtta agactcccaa aaaaggtgga attggtctta atgattcatt aaataacata
     5521 tgtaaattgg caaaagcaat agcgaataaa gaagatataa ctttgatgaa agaagaattt
     5581 tgttattaat aagagtatta attattagca agtttaaata ttatactatt tataataagc
     5641 atacattagt aaaatctata taatttaagg aggggaattt taacatgtgg ggaatgatag
     5701 cgacatggag aatggcccta gaaggaataa ctgaggcatc agaacattta gaaaaaggca
     5761 tggaagcagg tgatgctgta gagcttgctg taagaagagt tgaagactat ccattctata
     5821 aatcggttgg atatggtgga cttccaaatg aaaattgtga ggtagaactt gacgctgcat
     5881 ttatggatgg taacacatta gatattggcg cagttgctgg tataagagat tattctaatc
     5941 ctgtcagcat agctaggaag ttaagttatg agaaggtaaa taatttttta gttggaatag
     6001 gtgctgaaga ttatgctcat aaaaatggat ttgaaagaaa aaatatgttg actgatagag
     6061 cgaagttaca ttataaaaag agaaaaaaag agactttaga caaaggatta agcccatatg
     6121 ctggtcatga tactgttggt atgatatctt tagacaaaac tggaaaaatg tgtgcagcta
     6181 catcaacaag tggattgttt atgaagaaaa gaggtagaat aggagattct gcactatctg
     6241 gttcaggatt ctatgttgat agtagtatag gaggagctac agctactggt ttgggagaag
     6301 atttaatgaa gggatgtata tcttatgaga tagttaggct tatgaaagaa ggaaagcatc
     6361 cacaagaagc atgtgatatt gcagtaaatg aacttgataa acagcttatc aaaagaagag
     6421 gaaaagctgg tgatttatca ttagttgcca tgaataataa aggtgaatgg ggagtagcta
     6481 caaatatagt tgatttcact ttttcagtag taactaaaga agaatctcct gctgtatata
     6541 tttcaaatag agtagatgga aagactatac ataaggttat atcaaatgag tggatggaag
     6601 catatgaggc tagaataaaa gcaccgatag aagtaagtga gtaaagtagt gttacataat
     6661 taaaatagag ttttataata aggagatttt tatgaatatc ataagtaata aaatagatga
     6721 gttaaaagaa gatttattaa gtgatattat agatattgta aaaataccta gtgtcaaagg
     6781 ggagtcagaa aatggcttcc cttttggcga aaaagtaggg gaggcactta ataaagcatt
     6841 agaaatatca aaaaaattgg gatttaaggt tagaaacttg gataattata ttggttatgc
     6901 agagcatgga gatagtgatg attatgtttg tgtaattgga catgtagatg ttgtacatga
     6961 aggagatggt tggaagcatc agccttataa gggagaagaa actaatggaa gaatttatgg
     7021 acgaggagta ctagacaata aaggacctat aatgtctgcc ttatatggat tatatgctat
     7081 taaagaatta aacttaaaat tagataagag agttagaata atatttggta caaatgaaga
     7141 aagtggattt gaagatatac catattattt agaaaaagaa aaaacaccta ttatgggatt
     7201 tacaccagat tgtaaatatc ctgttgtata tggtgaaaaa ggcatggcaa agattagaat
     7261 aaaaagtaaa atcaattatg aagaagatgt atacctaggt tttatagaaa atatgtctga
     7321 aaatgtgctt gttacatata aagagctgaa tatagaaaat agtaatacta tattagatat
     7381 aaaggtaaaa tacgatttta gttataaatt gaaagatgta ttagatgaaa taaaagctag
     7441 ttttccaaat agtatcgata tagaggtaat ttctaatttt aatcctgtat attttgataa
     7501 agagagtaat cttgttaaaa agcttcagct agcttatgaa agggttacat cattagatgg
     7561 aactcccgtg acaactaatg gaggtacgta tgcaaaagta atgccaaata tagttccgtt
     7621 tggaccttca tttccaggac aaaaaggaat agctcataat cctgatgaat acatggatat
     7681 agaagatata atattaaatg caaagatatt tgcaaatgca atatatgaat tagcaaagga
     7741 gtagttatgt tagaaataat aggaatgtca gtagaagacg ctaaaattat agaagattgt
     7801 ggtgcagata gaatagagtt agttagtgct ttaactgaag gtggattgac accaagtttt
     7861 ggtcttatag aaagtgttgt taacagtgta aagatacctg tgaacgtaat gataagacat
     7921 catgcgaaaa gttttgtata cagtaaagaa gatataagta taatgcaaaa agatataggt
     7981 gtggttaaag agattggtgc taatggagtt gtatttggtg tattggataa aaacaataat
     8041 atagatgaga aaaatctaaa tgttttattg aaatgttgtg ataatcttga tgttacattt
     8101 catagagcca tagatgaatc aaatacaata gatagtgtta aaattttgaa ggattatgat
     8161 aaaattacga atatattaac atcaggaggg aaaggaagta tagttgataa catacaaatg
     8221 ataaagaata tgatgcttag ttctaatcac ataaaaattc ttttaggagg aggtcttaat
     8281 tttaataata tagaaaagat aaaggaatta actaaagcaa gtaattttca ttttggtaca
     8341 gcgattagaa taaataacag tccttttgaa gatatagata gacaaaagct taaacaactc
     8401 gtaaatataa tttcaagata gtatatgaat ataaaatact tagtttaaat aaagagagta
     8461 agttaattat aatttgggta tatgaatgtt aataggtatt aaatatgaaa ttattgttga
     8521 gtatgcttta taacttttaa gtagttttaa atataaataa tgaaagaaaa gatagattat
     8581 ggaggtttta gagggtttat agttattaaa aaattataaa ggtatattgg catataatac
     8641 acataatttc ctaaaattac catcttatga catgaattca catattaata aacaattaat
     8701 ggagattttt gtggatagaa ataggagtaa aaatagcttt aaataaacaa aaacaagagt
     8761 tattaacagt tttatccaca ttatccacag g
//
LOCUS       DAEPOG010000156         8772 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEPOG010000156 DAEPOG010000000
VERSION     DAEPOG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.156"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1446
                     /locus_tag="KR432_002977"
     CDS             <1..1446
                     /locus_tag="KR432_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0641493.1"
                     /translation="SRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEMLRE
                     VINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEEII
                     YLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLGYH
                     LSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYIAI
                     HFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLRNI
                     NLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNSTNL
                     LINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFDKG
                     LLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYEEV
                     LKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            1515..1874
                     /locus_tag="KR432_002978"
     CDS             1515..1874
                     /locus_tag="KR432_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641494.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            1909..2265
                     /locus_tag="KR432_002979"
     CDS             1909..2265
                     /locus_tag="KR432_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0641495.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            2302..3594
                     /locus_tag="KR432_002980"
     CDS             2302..3594
                     /locus_tag="KR432_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0641496.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            4030..4668
                     /locus_tag="KR432_002981"
     CDS             4030..4668
                     /locus_tag="KR432_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641497.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            4675..5517
                     /locus_tag="KR432_002982"
     CDS             4675..5517
                     /locus_tag="KR432_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBH0641498.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            5578..8205
                     /locus_tag="KR432_002983"
     CDS             5578..8205
                     /locus_tag="KR432_002983"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0641499.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            8419..8664
                     /locus_tag="KR432_002984"
     CDS             8419..8664
                     /locus_tag="KR432_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0641500.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         3555 a          883 c         1682 g         2652 t
ORIGIN      
        1 tcaaggaaag atttcagtaa ttcaaagtat tttaaaggaa tcaaaaaaga agaaatcata
       61 aatttagaag aaaaactatt gactttattt aaagaaaata atttttatgt tacagatgaa
      121 atgttaagag aagttattaa ctacatagta gttttagtct atagagtaaa agaagaaaag
      181 aaagtaaaag actatgatgt aaagtttgat ttacttaaat catatgatga atactttatt
      241 gctagggaaa taaaacatat aatatcagga atgtttgaat gtactttaaa tgatgaagaa
      301 attatttatt taactatacc attagttagt ggaaatgcac cagcatcaga atgtgcttta
      361 aatagtagta gaattagtaa aaacattgat gagttgatgg aaaatatatt taatcaaata
      421 tatgtcgata tgggtatttt tataaatgag gatgaattaa gagtaggtct aggatatcat
      481 cttagtttta ctttaaacag gctattattt aatataaagt taaaaaatgt tttgttagaa
      541 gagattaagc aaaattatat acttccattt aaattagcac aaatagcagg aaaagtaata
      601 gagaaaaagt ataacttaga agtttcagaa gatgagattg gatatatagc aatccatttc
      661 agtggatatt tagaaagaaa tagcagtaga ttctattcaa taaaaaaaat agcaattata
      721 tgtagtacag gtctaggaac agcaaaacta ttgaaaataa gagtagaaaa actgatagga
      781 aataatccta aaattgatac tctttcaagt tttaatttga gaaatattaa cttagatgaa
      841 tacgatatag tttttacaac tattgattta gacacatcga atataaatac aatagtatta
      901 aaaataaata caatatttga tgaaaacaaa ttaagagaac aattaaaaac agtattgtgc
      961 ctaagagaag ggaatattga cactactaat tctacaaatt tactaataaa taatttgtta
     1021 gatgaagata aatttatgat attaaatgaa aagactatac ttgcttcatt agaaaaaatg
     1081 atggaccatc ttatggattt gggatgtatt gatgataagt ttagaaaaaa tatagtcaat
     1141 agagaagaaa aatctcccac agtatttgat aaaggtttat tatttcctca ctctgtaaat
     1201 gaaaaatcag ataagttttt aatggcagtt ggaattttag aagaaccaat agaatatgca
     1261 aatagaagta taaaaataat tcttatgaca atgtttccat gtgagaataa gatagactca
     1321 gacttattag taaaaattta tgaagaagtt ttaaagatag gccaagatgt aaaattaacg
     1381 aataagataa gtaaatgtag aactttttta gaatttaaaa aggtgttact taaaaacctt
     1441 atataactat tgtacagcat ttatagttgt tatacagatt taaaaataat gtgatttttg
     1501 gaaggtgtat aaatatgaaa ttattagtaa ttttatttgt tgcttatatt ttaaatacta
     1561 tatttataat gaagcagaat aaattgtatt ttaaaacttt aaatagagct aaaaaaaagg
     1621 gtgatatagt gtctacaggc aaaaagaaaa gctatttttc taaaggaagt atagccatta
     1681 tatcttcaga ttcagatggg tttataaagt ttggagaaat tttaaaaggg cgtacagtaa
     1741 tggctaaatt taaagagatt gaagaaatag aaggattaga tatatatagt gcagagcaaa
     1801 aatttaaaga tgaagagagt attgtacaag ctataaaatt tataaaagaa aaaataaatg
     1861 tttcttttaa ttaactcaat ataaatataa attatcagga ggaatgtcat gaaatatgaa
     1921 gttaagataa gtgggatagg cagtttagtt aaagagttaa tggatgaaag caattgtctt
     1981 attatatatg atgaaaccat taatgatgac gacttaaagg atatatctgt aattcattcg
     2041 atagcaacat taaaaagtga tgttgaaata ggagatacac ttactatagg gaatagagat
     2101 tattgtatag tttcagttgg tgatattgct cagaagactc ttagagaaat tgggcattgt
     2161 acaatcaaat ttgatggaaa atgtgaagta aatttaccag gagaaataca tgtggaaata
     2221 ggaaatccag atataactat tggagattta ataactattt cttaatttga aaataaaatt
     2281 attataacag gaggaattgg tatgttagat ttaaacagaa aacttgcaaa attggaagag
     2341 gaaggtaaaa aaataaaagt agctcttata ggagctgggc atatgggaaa tggaatggta
     2401 agccaaatgg caaatatgaa aggtattgaa gcttctattg ttgtagatat aaatttggag
     2461 ttggctcata aagcttttac tgatgcagga atagatgaag aaattataga taatgtaaca
     2521 aatgaaaagg atgcagaaat aaaattacaa gaaggaaaag tattgacatg taatgacttt
     2581 cttgtggctt gtaaaactaa gtcaatagat gtagttatag atgcaactgg gggtattgca
     2641 attggtgcag agatagcatt aaactctatt ttaaataaaa aacatatagt tatgttaaat
     2701 gctgaaactg attgtgttgt tggaccaata cttaaaaaat tagcagatga tgctggagtg
     2761 atatttacag gttctgcagg agatgagcca ggtgcagtaa tggaattatt tgattttgca
     2821 gatgcaatgg ggtttgaagt tagagtgata ggtaaaggta aaaataataa attagattta
     2881 gattgtaatc ctgatacagt tagagaagaa gcagaaagaa aaggagcttc acctcatatg
     2941 atagcttctt ttaaagaagg aactaaaaca atggtagaaa tggctttaat gtgtaatgca
     3001 actggatttg tgcctgatgt aagaggtggt catggtattg aagctacagt taatgaagta
     3061 cctaagaaat atgcattaaa aagcgaaggt ggagtattag ataattatgg tgtagttgat
     3121 tttgtaaatg gtatagcacc tggagttttc gtagtagtag cccataaatt aaaagcagtt
     3181 aatgatgaac taaaatattt aagtatgggt gatggaccta attacatatt atatagacca
     3241 tatcatttat gtagtttaga gactccttta tcagcagcta tggcagtctt agaccataag
     3301 gctacaatag tacctaaagc tggactagta gcagaagtta tgactatagc taagaaagat
     3361 ttgaaaaaag gtgagtttat ggatggttat ggagcatata catgctatgg tactatagaa
     3421 aaatacgatg tagcaaaggc tatgaatgct gtacctattg gtttgataag taaaaagact
     3481 aaagttgtaa aagatattaa aaaaggtgaa gttataactt acgatatggt agagatagaa
     3541 aaagatacta ctttatatca tttaagacaa ttgcaagaaa aaatatttgg gtaagatttg
     3601 atggtaatat aaatagatat tagtaaaaag ggttgatggt tatttttaaa tcaacccttt
     3661 taaattttta tatattcttg ttaggatact ttctttaaaa gtatattata ggttatttgt
     3721 agtcgtaaaa attattttga attagttctg caattttata ttcacattga aacaatatta
     3781 ttaaaaattt taatataaaa tgaattaaaa tgaccaatta tgtattataa tattaactgt
     3841 aatactaata tagttctatt aattaaagaa atatccacca ttaatatata ataaatatct
     3901 ttaagcgaga gaacttagta aaaaatatat cgtattcaat aaaaaatctg tttacagaat
     3961 ggtatatata gttatataat atactatata agatgcgaaa agataaaaag tatagcacaa
     4021 tgaaagaagg tgattattat tcagcttaat gaaagacaac taaaaattat agatatagtt
     4081 aaggaaaatg aaccaataac gagtgaaagt attgcctcca gcttgaatgt gactcgtgct
     4141 acacttagat ctgatttagc tatattaaca atgacaggaa tattagacgc aagaccaaaa
     4201 gttggatatt tttattcagg agttagtgaa ataaatttaa ttggtaaaag tgtaaaagaa
     4261 aaaacagtag aggatataat gagcatgcct gtactagcta agaaggatga aagtatatat
     4321 gatgtaatag tcactatgtt tttatctgat gtagggagta tagttattat agatgagaat
     4381 gaagagttat gtggagttgt ttccagaaaa gacttgctaa aagcaactat aggaggttct
     4441 gatataaata aaatgccaat aggtatgata atgacaagaa ctccaaatgt agtcacttta
     4501 actaaaggag caagtgtttt attagcttca agaaaaataa tagaacatga agtagattcg
     4561 attccgattg ttgaatataa gggagaagac aaaaatcata tgagagttgt tgggagaata
     4621 tcaaaaacca acataactaa gctattctta gaaatagttg acaattaagg aggtatgctg
     4681 gtgaaaaacc taattatata tgcagtatca gattcagtag gggagactgc acaacaagtt
     4741 gcaaaggcat gtatgtctca gttttatgtg aatgaaactt atgaaataaa aagatttcca
     4801 tacatgatta ataaaggagt actacttgaa actttggaga atgcaaaagc tgaaaacgct
     4861 ttaattgtat atacattagt tgatgaagaa ttatgcagta ttgttgaaag atattgcgaa
     4921 agagaaggtc ttagttgtat agatttaatg acagatatac taagagaaat aagcaagaga
     4981 acaggaagaa aacctaaaag agaagctggt ataattagaa aattagatga atcttacttt
     5041 aagagagttg aagctataga atttgcagtg aaatatgatg atggaaaaga ccctagaggt
     5101 gttcttcaag cagacataat tttagtggga atatcaagaa catctaaaac tcctttgagt
     5161 atgtatttag ctaataaaaa tataaaagta gctaatgttc cattagttcc agaaatacca
     5221 attcctaaag aagtgtttga gatagatact aaaaagataa taggtcttac taattcacct
     5281 gaaaagctga atgagataag aacccaaaga ttaaaagctt taggattgtc cagtaaagca
     5341 aactacgcta atttagaaag aatacttcag gagttagatt attcagaaga gataatgaaa
     5401 agaattggat gcccagtaat aaacgtttca aataaggcaa tagaagaaac agcaggtata
     5461 attttagaca taatgaaaga aaatggtcta aagatataca aagaaataga aatttaataa
     5521 tgtctattaa taaaaatgat ataaattaaa ataaaaagat taatggggga tttaacaatg
     5581 gaaactaagt atgtttatag ttttggtgaa ggaagcaaag acatgaaatc tttactagga
     5641 ggtaaaggtg ccaatttagc agaaatgact aagataggct tacctgtccc tcctggattc
     5701 actataacaa cagaagcatg taatgattat tatgttaaca atgaaagtat aagagcagag
     5761 ataataaaag aaatagaaga gcatttagct actttagaaa aagacttaaa taaaacatta
     5821 ggatgcaata agaatccatt attagtatca gttcgttctg gtgcagtatt ctctatgcca
     5881 ggaatgatgg atacaattct taacctaggt cttaatgaca atagtgttgt tggcttagca
     5941 gaggctacac aaaatgaaag atttgcatat gatagttaca gaagattcat acaaatgttt
     6001 tctgatgttg ctatggaagt tccaaaatat aaatttgaaa atgtattaga cagagtaaaa
     6061 gaagcaaagg gatacacagt tgatacagaa cttacaactg atgatttaaa agaaatagta
     6121 aaagaattta aagcaattta taaaaaagaa ataaaaaatg atttcccaca agacccaaaa
     6181 gagcaattaa tgcttgcaat agaggctgta tttagatcat ggaataatcc tcgtgcaatc
     6241 gtataccgta aattgaatga tatagctcat aacttaggaa cagcagtaaa tattcaatca
     6301 atggtatttg gtaatatggg agaaactagt ggtacaggtg ttgcatttac tagaaatcca
     6361 gctacaggag aaaataaatt atttggtgaa tttttaatga atgcacaagg tgaagacgtt
     6421 gttgcaggta taagaactcc tcaaaatata tcaactcttg cagatgtaat gccagctgta
     6481 ttcgatgaat ttgtaaaaat tactcatata cttgaaggtc attataaaga tatgcaagat
     6541 atagagttta ctatagaaaa tgaaagatta tatatactac aaactagaaa tggtaagaga
     6601 acagcagcag cagcaataaa tgtagcggtt gatttagttg aagctggaat aattgatgaa
     6661 aaagaagcta tcatgagaat tgagcctaat caattagacc aattattaca tccaaaattt
     6721 gaagataagg cattaaaaga agccaaggtt atagcaaaag gtttaccagc atcaccaggt
     6781 gcagcaagtg gtaaagttta tttcaatgct gatgatgtag taaaagcaaa tgaaaaagga
     6841 gaaaaagtag ttttagttag attagagaca tctccagaag atatagaagg aatggttaaa
     6901 gcagaaggaa tccttacagc tagaggtgga atgacatcac atgcagcagt tgttgctaga
     6961 ggtatgggta aatgttgtgt tgctggatgt ggagaaataa aagttgatga gtttaataaa
     7021 gaaataagag cacttgatga tgtagttata aaagaaggtg aatacatttc tatagatggt
     7081 tctacaggaa atgtgtacct aggagatgtt aagaaaacag aagtatcttt aactggaaac
     7141 tttgaaaaat taatgaactg ggtagacaaa cacaaatgta tgatggttag aactaatgca
     7201 gataatccta gagatgctcg tgcagctata gagtttggtg cagaaggtat aggtctatgt
     7261 agaactgagc atatgttctt tgatgaagct agattgccag cagttagaga aatgatatta
     7321 tctaatacag tagaacaaag agaaaaagct cttgaaaaaa tattaccaat gcaaagagaa
     7381 gattttgtag agttatttaa agttatggat ggaaaaccag ttaatataag attactagac
     7441 ccaccactac atgaattttt gcctcatgat gatgagacta tagaagaact ttcaaaatct
     7501 atgggaataa aagtaagtga tattaagaag agaatagttg atttagatga gtttaaccca
     7561 atgcttggac acagaggttg tagacttgca ataacttatc ctgaaatatg tgtaatgcaa
     7621 tctaaagcta taatacaagg tgctatagaa gcaatcaaag caggtgttaa agtttctcca
     7681 gagataatgg ttccattagt tggagaagta aatgagttaa aaataataag aaaaatgata
     7741 gtagaaactg ttgacgctat aataaaagaa gaaggtatag aagttcctta tactgtaggt
     7801 actatgatag aaatacctag agcttgttta actgctgatg aaatagcaca agaagcagac
     7861 ttcttcagtt ttggtactaa tgacttaact caaatggcat ttggatattc aagagatgat
     7921 gctggtaaat tcttaggtca atatgtagat gaagaaatat tagaaaaaga cccattccaa
     7981 gtactagacc aaaatggtgt tggtaagtta gttaagatgg gagctaaatt aggtagagaa
     8041 gttaaaccag aattaaaact aggaatatgt ggagagcatg gaggagaacc atcttcagta
     8101 gagttctgtt atagtgtagg acttaactat gtatcttgtt caccatttag agtgccaatt
     8161 gctagacttg cagcagctca agcatctata aaaaatccta gataataata gactgcaaaa
     8221 tatgtaacta aataaaaata aaatccccta ttgttcaatg tatcaaatta agtatttgta
     8281 aattgaatat aggggatttt tattataaaa atttaactta tacagttgta agtgaaacgt
     8341 atttaattat attgcatata ttattgtaga gagaaatttc aactatcaat taaattgagt
     8401 ttactggagg ggataaagtt gacaaagtgt cctaaatgtg gaaaggaagt atcatcacgt
     8461 ccaggtagta tatgcccacg atgtggattt aaagtagcag atgagagtct aaaaataaga
     8521 tgtccagaac ctagttgtag ggctttggta tctaggaaac tggattattg tcctaaatgt
     8581 ggatgcaaac ttagaggata taatatttct gaatttgtta acatggtaag atgtaaaata
     8641 gatgagacat ttgataaaaa ataattaaat ttagagttaa gcttttgagg gtgtctcaaa
     8701 atgaactttt tagttcagaa ggcactcttt ttgtatggaa tcaaatatat ttttataatt
     8761 taaacaataa aa
//
LOCUS       DAEPOG010000157         8670 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEPOG010000157 DAEPOG010000000
VERSION     DAEPOG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.157"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     regulatory      48..150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            244..1620
                     /locus_tag="KR432_002985"
     CDS             244..1620
                     /locus_tag="KR432_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0641501.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1734..2879)
                     /locus_tag="KR432_002986"
     CDS             complement(1734..2879)
                     /locus_tag="KR432_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0641502.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            3131..3838
                     /locus_tag="KR432_002987"
     CDS             3131..3838
                     /locus_tag="KR432_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641503.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3885..5480
                     /locus_tag="KR432_002988"
     CDS             3885..5480
                     /locus_tag="KR432_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641504.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            5583..6665
                     /locus_tag="KR432_002989"
     CDS             5583..6665
                     /locus_tag="KR432_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0641505.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6814..6975
                     /locus_tag="KR432_002990"
     CDS             6814..6975
                     /locus_tag="KR432_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBH0641506.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7316..8584
                     /locus_tag="KR432_002991"
     CDS             7316..8584
                     /locus_tag="KR432_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0641507.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
BASE COUNT         3090 a          942 c         1366 g         3272 t
ORIGIN      
        1 attgacaaaa ttaattatat aaattaaaat aatattcgta aagaaaaatt catgttttgt
       61 actgtataac atataatttt gacaatatgg gtcataagtt tctaccggaa taccgtaaat
      121 attctgacta tgtatactat tatttagtta attaaatatt taattaactg aattatgaag
      181 gatacggctc agcttatcaa ctactgtgtc gtttttttgt aaaatttaat aggaggtgtt
      241 gttatgcaaa caacaaacag tagtactatg cagaaacttt ttccaatttt gactaataaa
      301 aatgtcgaca tgaagaagga aataattgca ggagtaacta cttttttaac tatggcttat
      361 ataatagctg taaatccaaa tattttatct gagacaggca tgcctgcagg tgcacttgtt
      421 acaggtactt gtttagcagc agcatttgga tgtattttaa tgggtgtagt tgctaacctt
      481 ccttttgctt tagcatcagg tatgggtctt aatgcatttt ttgcatatac agtagtttta
      541 caaatgggtg ttccttggga agtagctctt acagcagtat ttgtagaagg tattattttt
      601 atagtattat cagtaagtgg agttcgtgag gctgttgtaa atgcgatacc aaaaaatatg
      661 aaattagctg ttacaggtgg tataggaata tttattgcac ttataggtct agttaacagt
      721 ggaattgtta ttggagacca agccactctt atcaaaatgg gtagatttac accagcagta
      781 ataataactt gtttaggatt aataattatt gctgtattag ataaaaaaag agttaaaggt
      841 tctatacttt ttggaatagt agtgagttca ttacttgctt gggcatttgc ttttatgaat
      901 ccagagtatg cacaaaaatt aggtatatat ttacctggtg gattatttaa attcgaaagt
      961 ttagcaccaa tagcaggtaa aatagattta ggatatgtac tacatccaac aaatataggt
     1021 ggttttttag tagtagtttg tacattttta tttgttgatt tttttgatac agtaggtaca
     1081 ttagttggtg tttgttctaa agcaaacatg ttagatgaaa aagggaatgt tcctaatgta
     1141 ggtagagcct tacttacaga tgcggttgca actacagttg gtgcaggact tggagtatct
     1201 acagttacaa cttatgttga gagttcaaca ggagttatcg caggtggaag aactggatgg
     1261 acagcaataa cagttggaat tttattctta gctgcaatgt ttttctctcc tgtatttata
     1321 gcaattccat catgtgctac agcaccagct ttaatatatg ttggatattt aatgcttggt
     1381 acagttaagg atatagagtt tgataatata actgaaggtg ttccagcttt tgttactatt
     1441 gcttgtatgg cattaacata tagtataggt gatggtttaa ctttaggtat attaacttat
     1501 gttttcgtaa atatatttta taatatattt ggagctaaaa aggcagaaga caaaaaacat
     1561 gtttctgtag taatgattgt tctggcaata ttatttataa taaaactttg gctattataa
     1621 taggtattat ttaaaatttt aatttaatat aaaataaagg gctataattt attatcaatt
     1681 gtgatgataa actacagctc ttttgtattt gttgtatttt cgtatttatt gtattattta
     1741 ataatgtcta ttagttctcc attttgtaaa gttttaggtg taattactat actgaactct
     1801 ttttgcaaat cttttataat atctaattct ttttttgtta ttattgagat tgtgttacca
     1861 cttcgatttc ctctagaagt tcgaccacat ctatgcaggt attcattttt actttttgga
     1921 aaatccaagt taaaaacatg acttacatcc acaatatcta gtccacgagc agataaatca
     1981 gttgtaatta aaatctttgc tttaccaagt ttaaatttat ttattgcatt ctttctatct
     2041 tctttttcca tatttccaaa aattcctatt gctttgtaat tatgataatt tagttttgat
     2101 actaggactt ctatattttt ttcattattt acaaatacaa tagctttttt tggatttgta
     2161 gctgctaatg cctttctaag aaaagtaaat ttatccctta tctcacctaa taaatatgag
     2221 tgattgatgt taggatttat ttgtgatttt tcctttgttt ttattatttc aaactccttc
     2281 ataagtttat cacaaatttt tatagtgcta tcagtaagac ttgcagagca tcctataatt
     2341 tgtctatctc ttaacgttgt tcttattatg tcttctatac aagttatatt ttttccattc
     2401 aatagattgt ccacttcatc taagactata gtttttatat tatgtgattt tagcttcttt
     2461 tgttttatca agtctaaaac tcttccacaa gagccaatta ctatgtgtgg ttttactgct
     2521 tttatatttt ttatttgttt ttgtatattg acctccccaa ttaaagctaa agaagtaacg
     2581 gagagttctg catttttagc caataattca acctggtttg taatctgcat tactaattca
     2641 tgcgtaggtg caagtataag agcttgtgtt tctctttttg atgtatcaat tttttcaaat
     2701 ataggtaaaa ggtacgctag tgtttttcct gttccagtct gtgaattaat caataaatcc
     2761 ttattttgaa ttatattccc tataacaagt gactgaactt ctgttggaga agttatgtct
     2821 tgttttttaa gtccatctat tagagtggaa cttattttta attgttcaaa tgtattcata
     2881 ttttgctcct tatatttaat aattatagtc aactattttt tattatacat tgtttattca
     2941 aatcgaacta tatattcttt tataattatg tgtaatttga aagtaaatat caattatatt
     3001 tataccttta tatttctgat acctttgcat tttatagatt tctaaaataa acaaataaaa
     3061 gttgtaattg tttgatattt gttatatatt atatataaca aatatcaaaa ctataatatg
     3121 gagggaaatt atgttaaaag taatcaatgt atctaaatca tataaaaata aaaaagtagt
     3181 aagtagtgtc tcatttgatg taaatgatgg agagcttttt gggtttatag gacataatgg
     3241 agcaggtaaa actactacta ttaaagcaat tgttggaata cacgattttg aagagggaga
     3301 agtactaata aattctaaat ctataaaaaa acagcctgtt gaatgtaaaa aggaaatggc
     3361 atatatacct gataatccag atttatacga gtcattaacg ggtatacaat accttaattt
     3421 tatagctgac atatttgaag ttgccaaaga caaaagagaa gagttaataa aatattattc
     3481 aaataagttt gaactcaata aagcattagg agatttaata tcttcatatt cgcatggtat
     3541 gaaacaaaaa ttagctataa tttcggcttt gatacacagc ccaaaaatct taattcttga
     3601 tgaacctttt gtaggtcttg acccaaaagc ttcctttata ttgaaggaaa ttatgaggga
     3661 gttttgtgac aaaggtggtt gtatattttt ctcaacacac gttttagaag ttgctgaaaa
     3721 aatttgtgat aagattgcca taataaaaga tggaaatata attgcttatg gaactacaga
     3781 agaagttaag ggaagtaatt cacttgaaag tatctttatg gaattaatag agaaataaaa
     3841 tgttttttat acaatatagt gtctaaagtg aggaatgata aaatatgagc aatctaagta
     3901 tattaataaa aactaatata ataaatgaat ttaaattaaa tgcgttaaaa aaagctgata
     3961 gtagagaaaa acaaaaaata atggggatga tactttcagt tgttattata acagggttat
     4021 tgatttttta tgtaactagt ataagtcttt ctttagttga tattttaaag caaataaatc
     4081 agatggaaat gctacttata tttggatttg gattatcaac attaacaact attgttacat
     4141 cattatataa aacgtcatca tatctgtttc aagcaaaaga tttagacctt ttgactagtt
     4201 tacctataaa agaatctact gtactagcaa gtaagatatt aatgttggtt ggagcaaact
     4261 atttattttc ttcaatatgt atgattatac cagcagtggt ttattttttt aattctgatg
     4321 taagtataac gtatttccct tatttgatac tgctattttt agctttacca ttattaccaa
     4381 tagttgcttc atcaataatt tttatgttta taggacagat atcatcaaga ataaaataca
     4441 aaaatttaat attaattata ggtagtatta tattgatgtt gctttatact ctacttatga
     4501 caagacttaa ctctatgtcg gttgagatgt taaagaatag tgtatctatt tcagagacga
     4561 ttagaaaaat atatccacct atatattatt ttgttgacgc tcttaaaaca ggaagtgtag
     4621 tttccttttt agcatttata gcaatttcaa taataccatt ttttatgttt gtaacattat
     4681 ttgcaaaaaa atttaaagat ataaatgcta agatgagtga aggatataag tcaaaaactt
     4741 ataaatttaa gagccaaaaa acttcaagac cttcaaaagc tctattaaaa aaagaattta
     4801 agagatatac ttcttcattt atatactttt taaattcatc tgcaggtttg ttattcctaa
     4861 taataggtac aattttaata ttattatttg gagcagataa agtgggtgag ttactgaaat
     4921 tgaatataga ttttaatttt ataaaaatac aattattggg agtattcttc tttataatag
     4981 taatgaattg tacaacatac tgttcaattt ctcttgaagg aaaaaattta tggatactga
     5041 aatcttctcc aatagatgaa agagaaatat tctggtcaaa aatattgatg aattttttat
     5101 tgaatgcacc attatctatt ttatgtttaa tattagtatc tataagatta ggttttgaaa
     5161 ttcagtttat tattgtatca atctgtttga ttgcaagttt ttcaatgttt gtggcattga
     5221 tgggattatt atcaaattta ttatatccaa accttgattg gaaaaatgag atggctgtag
     5281 taaaaagaag tgcaagtatg ataattacaa tgttagtatc attagcttac gtaattatac
     5341 ttggaggtat atatgtatta cttggaattt cgtttttaaa tatatatatt attttggcaa
     5401 gtatacttac ttttatatta gattttatga tatggagaat tatttctact aaaggagtaa
     5461 gaatatttaa aaatttatag gtgtatttat gtcaaataaa gacttatctt ttttagtctt
     5521 gcaaataata ggttaagtag tatactatgt atagtgatat gaaaaaattt aggagaaaat
     5581 aaatgagaaa attatccaaa agagaaaaag attttattca atatggattt ttattatttt
     5641 tgatatgtat tactacttat ttagtattaa ctactttaga tataaaacta atacctaaaa
     5701 ttataaggct agtaaataag acttatatat ttcttggcat attgacaatt atagtatata
     5761 tgataattga atctattgta actcatttga taataaatgc agttcaaaaa acaaaagtaa
     5821 aatttattgg tattaaaatg gctacaatgg gattttacta taacttagtt actccatttg
     5881 catctgggag tcaaccaatg caaatatatg cacttacaaa atatgatgta agccttagta
     5941 aatcagtggc tatagttact aataaaactg tagtatttca aagtacagtt actgtatttt
     6001 gtggaatttt aattttgcta aataagtcat tactgatgga gcaagtacat tctgtgaaag
     6061 tcttgataac ttttggaatg attatgaatg taagtatgtt gttaggtgga ctattaatag
     6121 tatttagtcc aaaacaagta aaaatgatag cagaagtttt gattaatttt ttatctaaat
     6181 ttaaaacatt taaattttta gattcaaaac gtgatagaat aaatcattat attgatgatt
     6241 ataattattc tataaaaata tttattaaaa atgtaagaat cttagtttta agtatattac
     6301 ttactgtgtt acaattaatc gcatatttca gtatagttta ttgtgtgtat aaagcctcaa
     6361 atttaagagg agtttcatat gtacatataa tgacattaca ggtcttttta tatatggcta
     6421 tatcaccaat tccaacacca ggaaatgttg gagctaatga ggttacattt tttactatgt
     6481 tctccaatat aattccaaaa gaattattgg ggtactctgt actcttatat agtgggtttg
     6541 tatactattt tatattaata gtttcaggtt tatttacatt tggaacgcat tatacattaa
     6601 attcttggaa aaaggaagat aaagtaaatt ctaatttaga taaggttatt gaaaaattaa
     6661 attaataaaa taattaggga aattctatat ataaatatgg tgttgagtaa gctatattat
     6721 attgagatat ttcccttttt tgattaaaat tatagaaatt tgaaataata agtaataaac
     6781 agaaaaagtg atattgtttt gaaaggagaa actatgaaac aagataaaca aaatgaaaaa
     6841 caaagtgaga tttctaagat aaaacaaagc aatgataata aacttgaaat agctcaagaa
     6901 ttacatgtaa gtaaaaatat agggaatatg cttggatata gaatagataa gaaaaaaatg
     6961 aatagaccaa gttaatgaaa atgttgaaaa tggtacattt taaaaaatat atataatgaa
     7021 atgttttatt gttaaattta aacttatttt aatggaggaa aaagtcgaaa aatatctaaa
     7081 aagatatgga tataaaaaat attaatatat atatactata aacttgacaa aatcaacctt
     7141 ttaataacta gcaaaaaata gcaaaattat ataagcgtta tttaattatc aaaaatgaaa
     7201 gctaaattgc atgtcctaaa aaaaatgatt gataacattt atttgtgtgc tataattata
     7261 tagtaaaaca ataagtatat tgcataatgt atgcaaatcg ggaggagaga cggacatgga
     7321 agcatctata cacacaattg ccagcaataa tttattgata atttttgcta ttgtaagtat
     7381 aactggaata atttgtagta agcttagtga aataataaag gttccagatg tagtgttata
     7441 tttacttgta ggtattttaa taggaccatc atttttaaaa tttatagata ttcgtggatt
     7501 tcaaatagaa aatcaactaa tacttacatt tggttctgca tttattttat atttaggtgg
     7561 aaaagaaata agtttaaaag tattgagaaa tgtgaagata tctgtattct tattagctac
     7621 tttaggagtt gtgatatctg cttttataat gcaacaagta ataggattta cttttgggat
     7681 aagtgcaatg actgctttac tagcaggaac tataatagca tcaacagacc cagccactct
     7741 tgtacctata tttaatcagg taaagataaa ggatagggta aaacaaactg taataagtga
     7801 atcagctttt aatgatgcaa caggagctat actaacatca gcagtgttgg ctgtaatttt
     7861 gtcaaataaa ttttcattag gtgaaaatat ttatgaactg gggacaatgg ttattgtcgg
     7921 agttttagtt ggtttaatta caggaatact gcttttgaaa ttagttaatg ataaacctta
     7981 tggaatattt aaagattttg caccaataat atctgttatt tcagttgtag ttgcatatga
     8041 attatctaca aaattaggtg gaagtggata tatgtcttgc tttattgtag gtataataac
     8101 tggtaacaag aaaaacttta aaatttggtt aagtcaaaaa tcttatgatg cagattttta
     8161 tgttgctgaa actttaggta ctatttgtcg tatggcaata tttataatat tgggtagcca
     8221 agttaatttg gctcagttaa gtaaatattt tatgccatct gtaattacag tattagtatt
     8281 aatgtttata gcaagaccac tttgcgtttt agtttgtaca cttgtagaca gggaagcaaa
     8341 atggagtaaa agtgaaatat tatttatgat gtgggtaagg gaaacaggcg taataccagc
     8401 agcactttgt ggtattattt cagcaatgaa agttcctgga tacgaagtga tttcatctgt
     8461 agtatttatg actatattaa ttacattagt aatacaagga agtactacaa aattagtagc
     8521 taaaaaactt ggattattag aagaagaagt gataaatata agtgaaaaag tatcaacttt
     8581 ttaaagtttt gctttaaaat agtacctgtt aatggctatt ttaatattgt ctaatgatga
     8641 ttattgaaat agtcattttt ttatgtataa
//
LOCUS       DAEPOG010000158         8654 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEPOG010000158 DAEPOG010000000
VERSION     DAEPOG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.158"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(190..2310)
                     /gene="feoB"
                     /locus_tag="KR432_002992"
     CDS             complement(190..2310)
                     /gene="feoB"
                     /locus_tag="KR432_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0641508.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(2336..2563)
                     /locus_tag="KR432_002993"
     CDS             complement(2336..2563)
                     /locus_tag="KR432_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0641509.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(2890..3330)
                     /locus_tag="KR432_002994"
     CDS             complement(2890..3330)
                     /locus_tag="KR432_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641510.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            3482..4471
                     /locus_tag="KR432_002995"
     CDS             3482..4471
                     /locus_tag="KR432_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBH0641511.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    4623..4875
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4947..6155
                     /locus_tag="KR432_002996"
     CDS             4947..6155
                     /locus_tag="KR432_002996"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH0641512.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(6216..7097)
                     /locus_tag="KR432_002997"
     CDS             complement(6216..7097)
                     /locus_tag="KR432_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0641513.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            7483..8427
                     /locus_tag="KR432_002998"
     CDS             7483..8427
                     /locus_tag="KR432_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0641514.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3159 a         1142 c         1267 g         3086 t
ORIGIN      
        1 tgtgaaagta atgaagataa ttatatgact gaaatttcaa ttcaagtatc cagataagaa
       61 ttaaaataga gatatattca aatgttaagt aattttactt atcattgaat atatctctat
      121 gctttataat aaagtatata aatttttaaa tagtttctct aatatatgtc taatgtcagt
      181 taatactaat tatataaata aattgtaaac tgtagttgtt aaaaaacaaa caacaaaagc
      241 aagtatagta ggcattaaga atcctaaggc agtccatttt aaactaccag tttctttttt
      301 tattgttaaa agtgttgttg cacaaggcca atgcagtaaa ctgaacagca taacatttaa
      361 agctgtcaaa taagtccatc catgttctct aagaacttga cctaaagcac taaagctatc
      421 taattctatc atagaaccag ttgctaggta agccattaaa agtataggta caactatctc
      481 atttgctggg aatccaagaa taaaagcaag taatataaaa ccatcaagac caattaattt
      541 agctaaaggg tctaaaaagt ttgcaacatg tgataatatg cttaaatctc ctatatatat
      601 atttgcaaat atccaagtta tgacaccagc aggtatggct accattacag cccttcctaa
      661 aacaaatatt gttctatcta ttatagaggt atataaagtt cttcctattt gaggaactct
      721 ataaggagga agttctaatg taaatgtaga cggaactcct tttaatagcg tcttagaaag
      781 agtataagaa accaacaatg ttattataac acctagaatt atcagtaaag ttatgcaaag
      841 tgcagttgct acgcttgata cgaaagagtt ggttataact gagctaaaaa atattgtaga
      901 tatagctatc aaagtaggaa aacgaccatt acaaggaaca aagttgtttg taagtatagc
      961 tataagtctt tcccttggag agtcaattat cctacaacca ataactccag ctgcattaca
     1021 tccaaatccc atacacatag tcaaacactg ttttccatgt gcacaagctt ttttaaatag
     1081 gtggtctaag ttaaatgcaa ctcttggcaa atatccaaaa tcctcaagta gggtaaataa
     1141 tggaaaaaat attgccatag gaggtagcat tacagagatt acccatgcta atgttctgta
     1201 taatcctaaa accaacatat catttaacca tgatggacaa ttaatactat ttaatatacc
     1261 cgaaatactt ggctcaaatc cgagtaataa attagataat aaagtggatg gatagttagc
     1321 cccttctata gtaatccaca atatagtacc taataataag agcattattg gaagcccaaa
     1381 tattttagat gttaatattt tatctacttt ttcttctcta tcagttgatt ttttagcaac
     1441 attagaacaa cactcatcac tcagtttttt agcataatca tagttgattt tagtaaactc
     1501 atctcttatt ttctgtttat ttatattgtc tggaatcttt ttctttactt cattaatagc
     1561 atctatagaa tctttgtcta tataattact catagattca aatatacttt catcgccatc
     1621 aataagtcta agtcccaacc atctagaatt tatacctggt ataatattat ccaattctgg
     1681 ttgaattgat tttaccacat tctctatatt ttcattgtat ctaacaggtt tattatttaa
     1741 tttgtatttg tcaaaagata catcatttaa agtatccaat agttcatcca taccagagcc
     1801 atttcttgca gcagttaaaa taactggaat accaagagaa tcctctaaaa gttttttatc
     1861 aatagtaatg cctttttttc tagcttcatc tattaaatta atacacaaaa taactttatc
     1921 tgtaagttcc atcacttgaa atactaaatt taaatttctt tcaagacatg tagcatcaca
     1981 tactactatt actgcatctg gattaccaaa acatataaaa tcacgagcta caatttcttc
     2041 ttgtgataag gcaaataaag aatatgttcc tggtaaatct attaatgcat attcggtatt
     2101 tttatattta aaattaccac gagctgtagc tacagttttt cctggccagt ttccagtatg
     2161 ctgtctaagc cctgtaagat gattaaatac agtactttta cctgtatttg gattgccagc
     2221 caatgcaatt acaaactggt cttccttatt gtctatatca aacatatctt ttaaagaact
     2281 cattttagtt gagttatgtg tcaaccccat attaatatcc tcctgctaga aatttttaaa
     2341 tgtcagaaac taatattaag ctactttctt cttgtcgtag agctatctta ctacctctga
     2401 ttttatagac agttaaatta ttttttggac cttttctaac tacatctata acagctcctc
     2461 tagtaagacc aagcgctaac attctttctc ttaaattacc actagataat atatcttcaa
     2521 ctttgacagt ttttttcact tgaatatcgt taaggttttt catagtagca cctccatgct
     2581 tgaaatttag cctgggctaa tcttcttatt ctaaactatg cattatatta ttaccttgtt
     2641 acttttacaa aaaaaagata ggcaagatgc ctatcataca aagttgggta taattcttac
     2701 ttttggggaa agtagaatat agaataaaaa ttaacacttt gaatccatat aattattgaa
     2761 ttttgataat aaaaaagtca gaaagcaaaa gcctcctgac tctatgtaag gtattttcaa
     2821 atacctaaat atcaattcaa tatttaattt atacttaaat tatatattaa caatattagc
     2881 ttgtcaacac tataatctca ttttaatatg ttcaatacct gcttcgtcgt atacttctga
     2941 gatttcttta aaaccacaag ataagtaaag gttctttata taagtttgtg ctgataaggt
     3001 tatattattt tcgtgtaagt taatttttat aaaatctata gcacaatcca tcatttgttt
     3061 tgctaatcct tttcttctat gactagaaag aactaaaact ctaccaatgg aaacatcatt
     3121 ataagagctg tattctttag gaattattct agcataagca caaatcaatc cattttcttt
     3181 taaaaatatg tgataagagc ttttatctat atcatcataa tcgtttaatg aaaatatctt
     3241 ctgttcacaa gcaaatacct catatcttga ttttgctatt tcataaaatt catctaaact
     3301 caaatcatta aagtgtttaa ttttaaacat agtaattacc tcctagcatc ttaaaaaact
     3361 gaatattaag atacattata ccatttttaa cataaatacc tttattaaaa tttatattta
     3421 ttaaaaatat ttttaaaaag tgatatacta tattaaaaac aaaactttag ttagaaaatt
     3481 tatgatttat ggaggcagtg ttttgaatac tactaaaaaa ttaactgaag cagctttatt
     3541 gtcatcttta ttcatagtag taacaattat agctgtgagt actggatttg gatatgctat
     3601 ttacttagat tttatagtgc cagtattttt ttgtataatc tgcttgaaat gtgatttaaa
     3661 gtataccatt ttatctggaa ttacttcact tttaataatt tctctggtac ttggaaatct
     3721 aggaactgct atttgggcaa gtcagagtgt attacttggt ataatgtgtg gctatctaat
     3781 aaataagcct acaatggtta tggatgattt agtatatggt tcagtatttg gggttattgt
     3841 aatggttttc attgatatat atgcttcaac attaataggt tatagtttta tgaaagaatt
     3901 tcaaggatat agcaaatgga tttattttaa tgggtataca aattttattt attatttaat
     3961 gatagcattg tttccttttg gtatggtgtt ttgcatatat ttattaagtt taattttagg
     4021 gaataaattg catattttag attctaattc tttaaaaaaa tttttaatat ttaagaattt
     4081 tagaagttta aaccaatttt tatgttgctc aaaaaaagct ttttacatat gtgtttctta
     4141 tctagccatt tttgaagttc taaaactgtt gaatgttaaa gttgattctg tttatttaaa
     4201 aacaatcttt atatcaataa cctatataag tgtctatttt atcattagag actcatgtat
     4261 gtcactacaa aactttataa tagctaagtt tagaaaatta ttatatgcta gaattttatt
     4321 tttgataata attttattgc tgttttttat atttgatgta acagttatta gtttaataat
     4381 tataaacgca tttttaaata agaaaataaa tataagattg agtcagagta atatagtcaa
     4441 taaacaaata aatcttttga ttgaaaaata aactttgggt attggtaaaa aatataaaaa
     4501 agtagtataa tgtaaaaaaa catgtatata ataataaaaa aggttgacaa aatataaaaa
     4561 taataatata ttatgagtaa gttaagaaaa atttcttgta attgatgaaa taatctgaat
     4621 atataaatgc tatgaagaga agaagtaggg ctagatttta tttaacagag agttccagtt
     4681 tggtgaaatg gaataaattg atttagtttg aatacatctc agagcactat ctctgaaaga
     4741 taacaaccat taagagatat cggattagta cacgttatag tacacgagta tatgtaattg
     4801 tactctaaga gattaatatc gtgagatatt gatgaactaa ggtggtaaca cgtaagcaat
     4861 gctttcgtcc ttttaagagg atgaaagctt tttttattat aaaaaaaaaa ttatagatta
     4921 tataaatatt ttaggaggaa tgaaaaatga aatcgataga cgagcaaatg cgaataatta
     4981 tgaaaggtgt agatgattta atagatgaga aagagttaag ggaaaagctt attaaatcag
     5041 aaaaagaagg aaagcctatg atagtaaaat tgggtctaga cccaagtgct ccagatatac
     5101 atttagggca tacagtagtt cttagaaaga tgaaacaatt acaagattta ggtcatcaaa
     5161 tagtaataat aattggagat tttacaggaa aaattggaga cccaactgga aaatctaaag
     5221 ctagaaaagc tttaacaaca gagcaagttc ttgcaaatgc aaaaacttat gaagaacaaa
     5281 ttttcaaagt tttagataaa gaaaaaacta ttgtaagatt taatagcgag tggttagcca
     5341 agttaaattt tgaagatgta ataaaattag cagcaactat tactgttgcg agaatgttag
     5401 aaagagaaga ttttaaaaag agatatgaag gacaaatgcc aatatcagta catgagttct
     5461 tctacccatt aatgcaagca tatgactcta tagcattaga agctgatata gaacttggtg
     5521 gaactgacca aagattcaat ttactaatgg gaagatcatt acaaagagaa tttggtatgg
     5581 agtctcaaat agtaataatg atgcctttaa tagaaggatt agatggtaaa gaaaaaatga
     5641 gtaagagttt aggtaactat attggaatag atgaagaagc gggaataatg taccaaaaat
     5701 ctatggaaat tcctgatgaa cttataatta aatactacaa tttagtgaca gatgttcatc
     5761 ctgatgaagt taataaaata gaatctcagt tgaaagaggg ttcagtaaat ccaagagata
     5821 taaagatgaa cttagcaaga gaaatagtaa cattatacca tggagaagaa tctgctaaag
     5881 aagctgaaga aagatttaaa tctgtattcc aaaaaggtca aatacctgaa gatatacaaa
     5941 caattcaagt taaggaagat ggatttgact taatcgaagt actagtttct aatgagattg
     6001 ttaagagtaa gagtgaagtt agaagattag cttctcaagg tggagtaaag gtaaatggtg
     6061 aaaaagtaga ggatttaagc actattgtaa aagagagtga attagttgtt caaataggaa
     6121 agaaaaaatt tgtaaaaatc gagctagtaa aatagagtaa agtaaaagca agtctattga
     6181 gtataaaatg tacattcaat aaacttgctt ttttattata ttaaaatttt aaattgataa
     6241 ccattgtctt taaaatatgt tataatttgg tccaatgcat ttactgtttc ttgttttcca
     6301 taagtatcat gcattaaaag aacaaccatt tctttgcctt cagaagattt ctttgcatgt
     6361 tcaaataatt cttgtgcatt tttcttttta ccttctgcat cagcatttaa agcgttccaa
     6421 tcaattgatg ccatgttttt ctcttttaag tagtttccta gaggttccat atttttccat
     6481 gacatatacc cgcctgggca tctaactaca tttgaggaaa aattctttcc taagactttt
     6541 ttcatagctt catcagtttt gttaagttca tttacgaatg tatcgagatt taggcttctg
     6601 ccaggatata gttttttata gtcatgggta taagagtgat gggctattgc atttccttcg
     6661 tcaaatgttc tttttaaaat ttcattagct ccttttcttt caagagaatc acccttaata
     6721 aagaaagttc ctctaacacc atgtcttttc aagatatcta gaacttgtgg agtattagta
     6781 gtagatggtc catcatcaaa tgtaagaaag actatctttt ctccattgtt tgaatagtcg
     6841 tatttgttaa gtttgtccca aactattttt gcgtcatatc catattcttt tacagcttct
     6901 ttttttgcac caataatagg ttcatttttt tctttttcaa ttctttcttt ttctaatctt
     6961 tcagctttaa gtctttcttg agtttctttc atttcttttt ttctagacat gtccgcccat
     7021 gccatagaaa aaataccaat aactattaca acaagtatag taagtgtaac agttttcttt
     7081 tttctttttt ttaccataat tacagcattc ccccttttgt cgaattgtat caaaaatata
     7141 ttataattat atcgaagaaa gtagaaaaag taaaccgaca aagttgtaac taagataact
     7201 aaagtgtaac ttatatgaca ttatttgcat aatattaaag tatcatatat attaaaaata
     7261 tctataaaaa aatttagtat aggtaaaaaa atataaaaaa tataaaaatt tattaaaaag
     7321 gtgtttacaa aaaatgatta atatattact gttaaatagt ggatataaat ttgagctcaa
     7381 aatgtatgta aatttagatt taaaatactg aaaaaattta ctatattaat ttatagtaat
     7441 gcttatagaa aaactctctt gctgtttagg gggtttataa ttttgcaaaa aaataaaaag
     7501 gagggggctt tattgaaaaa atactttgga gaaataggac taatatttat agcaattata
     7561 tggggaagtg gctttgtagc aacccaattt gcattagacg gtgggcttac acctctacaa
     7621 atcataactt taagattttt cttggcagct ataattatga atttactgtt ttttaaacag
     7681 attcgtgcca atatgggtaa aaaattatta aaagcaggag ggattttagg aatattttta
     7741 tttctagcat tcacagtaca aactattgga cttatgtaca ctacaccatc taaaaatgca
     7801 tttataacag cagctaatgt tgtaatagtt ccgttcatag gatttatact ttatagaaga
     7861 aaacttgata aaataggaat aataagtagt ttggtagctc ttataggaat tgggatactt
     7921 tctttagaag ctgatttctc tattaatttt ggagattttc taacacttat atgttcattc
     7981 ggatttgcat ttcatatatt ctttacaagt gagtttgcaa aagataacaa cccaatggca
     8041 ttgacagcta ttcaatttac agtagcgttt ttaatgtctg ttgtagttca aacatttgca
     8101 ggtcaattaa agatggaagc tgaattatct ggttatatgg gaactatgta tctagctgta
     8161 tttagtacta caattggatt tttatttcag actatatgtc aaaaaagagt tgatggaact
     8221 agaacagcta taatattatc cacagaggct gtatttggta caatattttc tataataatt
     8281 ctaaaggaac ttattacagc aaaattgatt attggtagta tcttgatatt tgttgcaatc
     8341 ataactgcag aaactaaatt atctttttta aagagcaaaa aagtgaaact taaagattca
     8401 gaggagtctt cattagaaag tatttaatat gacaactatt tcctaattaa tcaaataagt
     8461 tatgtattat gtagaaattt gacatcaaaa cttggttgat actggtgttt caaagtgata
     8521 aaattaatta ggaaataaat tttattttta gtgtttaagc tataaagtct ggggtatata
     8581 tattcttgta gcaagttatt aaacttaaaa ataaaaattt aattatataa aattgggagg
     8641 aaattaatta tgaa
//
LOCUS       DAEPOG010000159         8489 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEPOG010000159 DAEPOG010000000
VERSION     DAEPOG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.159"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            175..1650
                     /locus_tag="KR432_002999"
     CDS             175..1650
                     /locus_tag="KR432_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0641515.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1737..2150
                     /gene="speD"
                     /locus_tag="KR432_003000"
     CDS             1737..2150
                     /gene="speD"
                     /locus_tag="KR432_003000"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH0641516.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2180..3031
                     /gene="speE"
                     /locus_tag="KR432_003001"
     CDS             2180..3031
                     /gene="speE"
                     /locus_tag="KR432_003001"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH0641517.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3021..3899
                     /gene="speB"
                     /locus_tag="KR432_003002"
     CDS             3021..3899
                     /gene="speB"
                     /locus_tag="KR432_003002"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH0641518.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4079..4279)
                     /locus_tag="KR432_003003"
     CDS             complement(4079..4279)
                     /locus_tag="KR432_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0641519.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4746..6236
                     /locus_tag="KR432_003004"
     CDS             4746..6236
                     /locus_tag="KR432_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0641520.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6494..7990
                     /locus_tag="KR432_003005"
     CDS             6494..7990
                     /locus_tag="KR432_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0641521.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8124..8474
                     /locus_tag="KR432_003006"
     CDS             8124..8474
                     /locus_tag="KR432_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0641522.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         3380 a          901 c         1542 g         2666 t
ORIGIN      
        1 ttgatatagc taatgtatat gtttttgagt aacttcaaaa aaacacttgt aaaaatgctg
       61 gaatgttaat ataataaata tcatgagttt aataaaagta aaaataaaaa atagaaataa
      121 taaataataa atattaaaaa tatttgaata tagagaggag atgaatttga aatcatggat
      181 agcgagtttg caaatgcagt tgaattaagc tatgagtgtg ctccattatt agagtcgtta
      241 aaggaatact cagagaaaga tatagcttgt tttgatgttc ctggacatgt aaaaaataga
      301 ggtgtagcta tattaaataa atatctaggt gaaagtctca tgaaaatgga tataaattca
      361 tcaccaacta tggataatgt atctgcgcca aatgggatta taaaaaatgc acaagatttg
      421 ctagcacaag catatatggc tgatgaggca ttctttataa cgaatggcac aactcaagca
      481 atacatgcaa tgattttgag tgttataaaa ccaggtgaaa aagtattact tccaagaaat
      541 atacataaat cagtaataaa tgcgttaata ctttgtggag gaataccaat atttatacaa
      601 ccagaatttg atgaaaaact aggtataagt ttaaatatta ctcttgaaaa agttagaaca
      661 gagatagaaa aagattgtaa tataaaagca ttatttttcc ttaatccaac ttactatgga
      721 gtttgtgcag accttgagag tattatagaa ctatgccata aaaataatgt tttagtgtta
      781 gttgatgaag ctcatggtgc acattttcca tttcatttag atttacctcc ttctgcaata
      841 agtttaggag ctgacatggt agctgtaagt atacataaaa ctggaggagc attgacacaa
      901 tcttcagcac tcttattgaa tagagataat gtaagttttg aaaaggttct tcaatcaatc
      961 aatatgctac aatcaacatc agcatcttat ttgcttatgg caagtataga tggtgctaga
     1021 gttaatctag ttgagaatgg tgaaaagcaa ctgtcaaaag cattaaatct atctaggtat
     1081 gcaaaatcta aattgaacaa aattgatgga ataaaagttt tatctacaga aatattgaaa
     1141 caaaaaggtg ttaaatttat agatgaaaca aaattgtgta taaatgtaaa agaattaaat
     1201 ttgactggat ttgaagtata cgatttatta tataaaaatt tttcaattca agtagaattg
     1261 ggagattcat ataatatact ggcacttgtg tccattggaa caaataaatc agatatagat
     1321 agattagtaa aagcactttc tataattgca aagatttata gaaaagaatc aacattaaat
     1381 gaatttaata tggttcaaat aaatccaatt attaaactta atccaagaga agctttttat
     1441 gcaccaaagg aaagtgtaga aatcaattca tgtatagata gaatttgtgg agaatcaata
     1501 atggcatatc caccaggtat accgattatt gctccaggag aattgataac agaagaaatt
     1561 atggagtata ttattttctt aaaaaatagc aatgcgtatc ttacagatgt acaagataaa
     1621 aacttagata gaatactagt aataaaataa attactttat tttgcaaggg caagtgtttg
     1681 ttaagtagag tttatcaaat agaacttatt aaaagtataa aggagttggg gttgaaatga
     1741 aatttgaaac actaggaaga catatactag tagaatacta taattgtgat gaaaacgtac
     1801 ttaaagaccc acgtcttata gaaaatttta tgaatgattc agcattaaat gcaaaagcga
     1861 caattgtgga ttctgtattc caccatttta atccttgggg ggtttcagga gctgttataa
     1921 ttgctgaatc tcatttgaca atacatacat ggcctgaata tggttatgca gcagcagatt
     1981 tttttacttg tggtgatata gacccatgga aaagttttga attattagag caacttttaa
     2041 agtctgaaag aagcgagtct acagaaattc caagaggctt aactacaaaa attaaaaaat
     2101 attcaaaaaa agatttaggt aagattactc ataaaccaga agcagtataa aacatacaag
     2161 agaaagtgag gattattata tggaactttg gtatacagaa gaatggacag aaaatgtaag
     2221 attttctata aaagtaaata aacatctatt tgaaggaaag tctcaatttc aaaggataga
     2281 tgtatttgat agtgatgaat ttggaaaatt tctaacaata gatggattaa tgatggtaac
     2341 atataaagat gaatttatat accatgagat gataactcat gttcctatgg ctacaaattt
     2401 aaacataaag aaagtattag tcataggtgg aggtgatgga ggtacagtaa gagaattaag
     2461 ccgttatcct caaatagaaa agattgacat ggttgaaata gataaaatgg ttgtagatgt
     2521 atctaaggag tatatggata tatgctcatg taaattagat gataaaagag tgagtttata
     2581 ttttgaagat ggtgttaatt ttgttaagtg tgcacatgat aaatcatatg acctgataat
     2641 agtagattca actgacccaa taggaccagg cgaagggctt ttctcaactg atttttataa
     2701 ggattgttat agaatactga ctgatgatgg aattttagta aatcaaagtg aaagtccgta
     2761 ttttgacttt aatgctaaag agatgaaaag agctaataaa aaattaaaac agatatttcc
     2821 aatatcagaa gtttatcaag cacatatacc aacatatcca tctggtcact ggttgtttgg
     2881 atttgcttca aaaaaactta atccagtaaa aaatcaagat agaaatggat gggagaaatt
     2941 aagtttaaaa actaaatatt ataatagtga tatacattta ggttcgttta tgttacctca
     3001 atacgtgaag gagatgctag atgaagaata atttctatca tatgaacact tttatgagta
     3061 tggacaaaaa ttatgaagaa tctaatctta tagtatttgg tgttggattt gatggaacta
     3121 cttctaacag acctggggct agatttgcaa gtagctctat gagaaaagaa ttttacggtc
     3181 ttgagacata tagtcctttt ttagatttgg acttagagga ttataatata tgtgattatg
     3241 gagatttaga aattagtgtt ggaagtacag aacaagtctt aaaagaaatc tatcaagaga
     3301 catataagat tgttagagat tcaaaggtac cctttatgat tggaggagag catttagtta
     3361 cattaccagc ctttaaagca gtacatgaaa agtacaatga tatatatgta attcattttg
     3421 atgcccatac tgatttgagg gaagaatata ataatagtaa aaattctcat gcaacagtaa
     3481 ttaaaagaat atgggatatt gtaggtgata ataaaatatt tcaatttggt ataaggtctg
     3541 ggacaaaaga agaatttaaa tttgctacag aagaaaaaca cacatacatg gaaataggag
     3601 gaatagatac gtttgaaaat atagttaata tgctaaatgg aaagaatatt tatctaacta
     3661 tagatttaga tgtattggat gcatctgtgt ttccgggaac aggtacacca gaacctggtg
     3721 gtgtaaatta tagagagttt caagagattt ttaagattat aaaaaactct aatataaata
     3781 tagttggttg tgatattgta gagttaagcc ctgattacga tacaacaggt gtatcgacag
     3841 ttatagcttg taaaatctta agagagttat gcttaataat atctgataaa attaaataga
     3901 gtattttata taaatagtat atattataat ataagaagtt attttaaaaa agttagttta
     3961 agtttgacgc ttttctaaag taacttctta tttttgtaat taaaaataaa tataataaac
     4021 aacaaaaaag agccctcgat agctcctttt ctaaaatata tatatttaaa ttaaataatt
     4081 atattctatt tacgttttca gcttgaggtc ctctattacc ttgagttata tcaaagctaa
     4141 ccttttcgcc ttcttctaaa gttttgtatc catcattttg tatagctgag aaatgtacga
     4201 aaacatcatc ttctccctct acagatatga acccaaatcc tttttcattg ttaaaccatt
     4261 ttactattcc gtttttcatt ataagaaaac ctccgcatta ttaaaaaata caaactaaaa
     4321 aaataagtct gttctattaa tataacataa attttaaata aaagcaaata aaatttttaa
     4381 aaaaattaat tacttttaga atttaataaa aattgttaaa ttatacagtg agaagtattg
     4441 taaaatttga cgaaaatgta aatgtagata ataaatttaa tgtcaaaaaa tgcaacaaaa
     4501 aggtatgtaa gtaaaaagta aaattaaaat atataataaa aaatattata tattagatta
     4561 aaaagtaaaa ataaaaatag tgaaatttca gaaatatcaa taatattata taataaaata
     4621 aaaaaacact atatatattc gataaaataa aatttaaaca tataatattt tatgtatata
     4681 ttaataaaag gacatattgt gttataatgg tatcgtataa aatcatttac gggggtggta
     4741 ttaaaatgtt tcaatttgaa acacaaatac acaaattaag acatgatgtt ttaactgcaa
     4801 tagctagact tggaaaagaa gataacctta ccagagaagc aatggataat attcagtatg
     4861 aaataataaa aggtgagaaa cctacataca gatgttgtgt ttataaagaa agagctataa
     4921 tatctgagag agtaaatgta agtatggggt tacaaccagg tcaagataag atagatttag
     4981 atacaatgga tatagaagaa gatgaacaaa ttatatatat tttggaagct gcatgtgaca
     5041 gttgtccaat aaatgaattt acagtaacag atatttgtag aggatgtcta gcacatagat
     5101 gtaaagaagc ttgtaaattt ggagctataa cacatgtcgg aggaatggcc tatataaatc
     5161 atgaattatg taaggcttgt ggaatgtgta aaaaagcttg tcaatatgat gccatctcag
     5221 aggttgttag accatgtaag agtgtttgtc caactaatgc cttaggattt gatagagaaa
     5281 atatgaaagc tatgatacat gaggataaat gtttaaactg tggagcatgt atgtcagcat
     5341 gtccatttgg agcaatatct gataagagtt tgatagctcc tgtagctaga aagttggttc
     5401 aaaaagagaa aatgtatgcg gtagtagctc cagctataac aggtcaagtt gaagcaaatg
     5461 ttacatatgg tcaaataaaa aatgctataa aatcactagg atttgttgat gtattagaag
     5521 cagcttgtgg agcagatgca gttacagttc atgaaagctt agaatttgct gaaagaatgg
     5581 aaaagggaga ggcttttatg acaaattctt gttgtccagg atttgttggt tatatagaaa
     5641 aaacataccc aaatcatgta ggaaaaatat cttctacagt gtctcctatg atagctagtg
     5701 gtagatttat aaaaagtcat gaaccagatg ctaaagttgt attcatagga ccatgtacag
     5761 ccaaaaagag tgaggcagct aagaaagaat taaaaggagc tgttgattat gtacttactt
     5821 ttgaagaaat aatggcatta tttgaggcat ttgatataga cttggctaag tgttcaaatg
     5881 aagatataga tgatgcatca atttttggaa gaggttttgg agctgctggt ggtcttacaa
     5941 aagctataga aaattatctt gctgaaaaag gtattggtgt taattttgaa cctgtaaaga
     6001 tttctggttc aagagaaatc aagaaaacta tgactatggc taatttagga aagctagaag
     6061 gtaattttat agaaggaatg atgtgtgaag gtggttgtat aaatggttct ggtaaattct
     6121 tatcaggtgc aaaggttaaa gccacttttg atagaaaaaa ttctcaatca actaagaaaa
     6181 gtgtcttagc aaatacaaag ataaaagatt ttagtaatat aaatttagaa aaataaagat
     6241 aatacaaagc aacaaaaata catattgtta atagagttgt tctaagttga ggtttaactt
     6301 ttggacaact ctatttttat ttatatattt aattaatgaa caactaaaat aaaaattaca
     6361 tatataatat tttgtatata acttaacaaa gtatatgcct atgttataat aatttataga
     6421 aataaataaa aaatacaata tacataatag tagaaatata gtaaaaaggt gtgatttata
     6481 gggaggatta cttatgtttg aatttgaaac gcaactaaaa aggttgaaac atgaagtttt
     6541 aacagcagta gcaagactat ctattgaaga aaatttaaca aaagaaaata tagaaaaaat
     6601 tccatatgaa ataattaaag gagaaaaggc gagatataga tgttgtgttt ataaagagag
     6661 agaagtagtt cttgaaaggg cgcaacttgc attaagttta actccaaata gtaaatatgg
     6721 agatataaat ccagattcag ttagtttgga cacagatgag caaattatat atatagttga
     6781 agcagcatgt gatagatgtc caataaatga atttacagtt acagagcttt gtagagggtg
     6841 tttggctcat agatgtaagg aagcatgtaa atttggggca atttcttata taaatgggag
     6901 agcatatata aatcatgatt tatgtaaggc ttgtggaatg tgcaaaagtt catgtcaata
     6961 tgatgctata tcagaagttg taagaccttg taaaagcgtt tgtccaacag gagctttgga
     7021 ttttaataga aacactatga aagcaatgat acatgaagat aattgtataa attgtggagc
     7081 atgtatatca gcatgtcctt ttggagcaat atctgataag agtttgatag cacctgttgc
     7141 aaaaaaactt gcaaagaaag aaaatatgta tgcaatagta gctccagcta taacaggtca
     7201 aatagatagt aatattacat atggtcagtt aaaaaatgct ataaaatcac ttggatttgt
     7261 agatatgata gaagcttctt gtggagcaga tgcagttaca gttcatgaaa gcaatgagct
     7321 tattgaaaga ataaaatcag gagataatta catgacaaat tcatgttgtc cgggattttt
     7381 taagtatata gagaagatgt tcccagatga aaaagataga atatcatcta cagtgtctcc
     7441 tatggtagca acaggaagat tagtaaaaaa aatagattct gatgctaaag ttgtatttat
     7501 agggccatgt acagctaaaa agagtgaggc aacagagaaa agtttaaaag attcagtaga
     7561 ttatgtactt acttttgaag aactaatggc attatttgaa gcatttaata tagatttatc
     7621 aaaatgttca aatgaagata taaatgatgg ttcgattttt ggaaggggat ttggagcgtc
     7681 tggtggactt gccaaatcag tacagaacta tattgctgaa aagggactta gttttgagtt
     7741 tgaacctata aaagtatctg gtccaagaca aattaaaaag acaatgacaa tggctaaatt
     7801 gggaagatta gaaggtaatt ttatagaagg aatgatgtgt gaagaaggtt gtattaatgg
     7861 agcaggtaag tttttatcag gtgtaaaagc taaaaatact tttgatagaa aaaatggaca
     7921 atctactaaa aagagtgttt tatctaatga taagattaaa gaatttgaag atgtaaattt
     7981 agaaagataa ttgttttggt acagtcattt ttttatcatg ccacaaatat gcctaaaatt
     8041 ttcccttttt atatgtgtat tttacagagt taaggttgct atactataaa tatgtaataa
     8101 ataaaattta taggagcttt tggatgaaga aaatagtatt aaaagatatc ctgtttttag
     8161 ttactacatt atctataata gtaatattat tgtttgtaac aaatataact actaatagta
     8221 agataaaatc aacaagtgaa tcttatttaa aaagtgacaa aaatagtgtg aggcaaaatg
     8281 gtatccagag gagcacaaaa aatactgcat ttaagactac tatattaagt actggaaagt
     8341 ctgactgtat attaatcgag ataggtgata aattcataat gattgataca ggagaagata
     8401 aaaatggaga acaaatagtt gataaattaa aagaaaaaga aataaatgca cttgactatt
     8461 tgattaaaaa atagctacgc acactcgtg
//
LOCUS       DAEPOG010000160         8431 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEPOG010000160 DAEPOG010000000
VERSION     DAEPOG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.160"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1764)
                     /locus_tag="KR432_003007"
     CDS             complement(<1..1764)
                     /locus_tag="KR432_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH0641523.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIK"
     gene            1877..2308
                     /gene="dut"
                     /locus_tag="KR432_003008"
     CDS             1877..2308
                     /gene="dut"
                     /locus_tag="KR432_003008"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH0641524.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2568..3758
                     /locus_tag="KR432_003009"
     CDS             2568..3758
                     /locus_tag="KR432_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0641525.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(3992..4564)
                     /locus_tag="KR432_003010"
     CDS             complement(3992..4564)
                     /locus_tag="KR432_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0641526.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(4593..4859)
                     /locus_tag="KR432_003011"
     CDS             complement(4593..4859)
                     /locus_tag="KR432_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0641527.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(4859..5068)
                     /locus_tag="KR432_003012"
     CDS             complement(4859..5068)
                     /locus_tag="KR432_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0641528.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            5347..6207
                     /locus_tag="KR432_003013"
     CDS             5347..6207
                     /locus_tag="KR432_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH0641529.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            6255..7283
                     /locus_tag="KR432_003014"
     CDS             6255..7283
                     /locus_tag="KR432_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0641530.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            7654..8160
                     /locus_tag="KR432_003015"
     CDS             7654..8160
                     /locus_tag="KR432_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBH0641531.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT         3179 a         1020 c         1347 g         2885 t
ORIGIN      
        1 ttttataata ttgacatttt gatatataaa actaatgata ttagtaagat attcttttct
       61 tgtatacttt cctttttgaa tatcgcttaa acttttttca agttttccag tataatctac
      121 atcaaataag tctttaacag gaaaaatttc aactaaattt ttcccaaggt ctgttataga
      181 ataagatttc ccttttttct ttacatagcc cacttgactt atcttcttta acacatcagc
      241 acgtgtggct gatgttccaa tcgaatatcc agatagcact gttgtatctt cttcaggcac
      301 atttttacca caatttttca tagcctttaa aagggtgtcc tcagtatatg gtttaggtgg
      361 agtagtttgc ttagttaatg gttttatttc taacacatct acaacatcat ctttatttac
      421 aagtggaagt aaatcgtttt tttcttcctt attatatacc tcaaggtatc cttttgattt
      481 aagcacttta ccttttgtta agaaagtaca attatctact tcagtcttaa tttctgtatt
      541 ctcatattca gcaggaggca taaagtttgc tacaaatcta tcttttatgg catcataaac
      601 taattgttca tccttagata aactttttgg aattatataa gttggaatta tagcactatg
      661 ggagtctact ttagaagaat caaaaactct tttagtttta gaaaatttaa ttttattttc
      721 atattcaaga ccaaccttta atttatctaa agtttgagaa gcttttgaag ccaaactttc
      781 ttctaagaaa atggaatcag ttctagggta agttatgtat ccacccttac cattaccctc
      841 atagagagat tgacagacat ttaaaacctt atcagatgta aaattggaat acttagaagt
      901 tatatatcct tgaagtgatg tcaaactaaa aagttttggt gcatattcct ttgacatagt
      961 aactttttta tctagtattt taccagtttc agatgtaatt gaagctataa ttttatttgc
     1021 atcttcaagt gtatcaaatt tactttcttt acccttaaca tatttacctt tatattcacc
     1081 agcttcagct ttaaaatgtc cttctatttc ataataagtt ttaggaacaa agtttagtat
     1141 ttccatatct ctatcataga ctaatttaac tgttggaaga attaccctac ctatgtttaa
     1201 tagtttccca tttccatatt tcaatgtagc aacagaagtg aaatttattc caatcaacca
     1261 gtcagtaata agcctagtat atccagcagc ttgaagattt ctcatttcat attcatcttt
     1321 taggtttttt atacctcttg taatatcttc tggtgtccat tcatttacca agattctttt
     1381 tactggtttt ttattttttg ccagtaaaaa tattaaaaat gctataagtt ctccttctcg
     1441 gtcattgtca gtggcattta ttacatattc tacatctttt ctattcaaaa gcttttttac
     1501 agtgttgaat tgtttagttt tagaactatc aatcttaaat ttaaacttat cctcaggaac
     1561 aaatgggaaa ttgttcattt tccaagagcc agagtattta tctttatcat aatccttcat
     1621 atcatataat gatactaagt gtccaactgc ataagtaatt atataatcat ttccttcaaa
     1681 gtaaccatct tgtctggtct ttgcacctaa aaatgatgct atagtttttg ccacagatgg
     1741 tttctcagca agtacaagtt tcataaaaaa tcctccttta aaacactaat aattatatta
     1801 ccacaaaaaa ataaaaaact tcaagaggtc gaaatttaag ttataatatt tatagaaagt
     1861 tgaaaggaga tataagatgt acaatttaaa agtaaaaaaa ttaaatgatg atgctataat
     1921 acctaatttt gctcacaaag gtgatgctgg tatggatttg tattctatag aagaggttgt
     1981 aatacctcct ggagagacta aacttatcaa aactgggata tgtatagagc ttcctactat
     2041 gacagaagcg caggtaagac ctagaagtgg gcttgcctta aagcattctg ttactgtatt
     2101 aaatacacca ggaactattg acgaaggata cagaggagag cttaaaatta tattaataaa
     2161 tcatgggaaa aatgatttta aggttgaaaa gcatatgaaa attgctcaaa tgatagtaaa
     2221 acctatctat gatattaaca tagaagaagt aaaagaatta agtgactccg aaagaggaaa
     2281 aggtgggttt ggctcaactg gattttaaaa agttacaaaa aagttaaaaa atatagccat
     2341 ttttaaaaaa ggtaaaagat ggctatatta ctaaattaca ggctattgaa gattaaaatt
     2401 tatacatggt aatttatggt aagcaaatat tactatagac aaatagttac aaatttgtaa
     2461 taatattaat ataaaagaaa taattgatat tttaatagtg tcaattattt cttttatatt
     2521 ttgaagtgaa ataaagatag tagaaaagag gagatgagtg gaaagttatg aattttaatc
     2581 aaaagagaat agcagccagt ataatggcaa ctgcgataat aatgcctact atggggaatt
     2641 tagcatatgc taatgagtct gaagtagaga gtgtgagtat agaaagcaga acaataacag
     2701 ggaatgcggt aaattttaga aaaggaccag gtacaaacca tgaatcaatg gggaaacttt
     2761 acaaaggtga taaagttgag tacgtaggaa aagaaggttc ttgggtaaaa gttaaatata
     2821 atggaaacac aggttatgtg catggaaatt atgtagcaat aaatagcctt ggaagtagca
     2881 atgaaagtag tgatacttca gttaagtcga caaaagttgt aacagcaaaa ggattaaatt
     2941 ttagaacagg accaagtaca agtagttcta aaatatcaac actaggatat gggactgaag
     3001 tcggatacat atctgagtca aatggatggt caaaaataag ttctaatggt agagtaggtt
     3061 atgtttcaag taaatattta ggaactagtg taaatgactc tacaaatgaa aatgttgaaa
     3121 acagttcaaa tgaccttgta aagggtacaa aggttgtaac agcaaagtca ttgaacgtta
     3181 gaacaggacc aggtacaagt cattctaaaa tagctacatt aagttatggt actgaggttg
     3241 gaagtatttc tgaaagtgga ggatggacaa aagtaagtta tggcaatcaa actggatatg
     3301 tgtcaagtca ataccttgct gaaaaaggtt ctgttgatac ttcaattcca tcttattcga
     3361 ctaattctcc aagtcaagga gctgatagtg taataagttt tgcaaagaca ctacttggaa
     3421 aaccatatgt atggggagca gaaggtccaa atagttttga ctgttctgga ttcacacagt
     3481 acgttatgaa aaaatctgca ggagttagta taccaagagt atctagagac caaagtaagt
     3541 atggaacata tgtaaataga ggagacctta gaagtggaga tttagtattc tttgatacac
     3601 aaggttcaaa taatggttct gtaagtcatg ttggaatata cataggaaat ggagatatga
     3661 tacatgcatc ttcaggttca tcaaaaaaag taacaatatc taatataaat agtagctact
     3721 attcaagtag atatgtaaat gcaagaagag ttttataatt gttttattat aaaacctaat
     3781 aaatagattt ttctttattg cttataatat gaaagcaggt acaatattga tagtaatgta
     3841 tagtataata catgttacta tttgattgat acctgctttt tatttatttt ctctgtttat
     3901 agaaaataaa ttatatttat attttttata aattttaaca caacagtaaa ttataaatta
     3961 tcaaataata atagttatac aaataaaaat tttagaacat tttttgagat tcttctaact
     4021 cgtaagcatc cattaaagct tcaccaaatc tttgatagtg aattatttct ctttgaccta
     4081 agaattttaa tacatctata acatcaacgt catctgttag gtttataagt tgataataag
     4141 ttgcaagtgc tttttgttca gctgccatat cctcatgtaa gtcggttacg ggatttgaca
     4201 taactgctat aggtcttaca tcaaatggaa caccatttga atctgttgga taaattccat
     4261 atccattttg agcatagttt gaaccaagac ctgcagcttt taactcttct gggcttgcat
     4321 cagaagttaa ttgataaacc atagtacata taagctcaac gtgagctagc tcttctgtac
     4381 caatatctgt taaaagtgca cgcatattac cagtaggcat tgtatatctt tggcttaaat
     4441 atcttaaaga tgcagcaagt tctccatttg gcccaccaaa ttgagttata agaaatttag
     4501 ccattctagg gtcacaagtt tttatattaa ctgggtgttg tatagttttc tgatatatcc
     4561 acatgaattt tcaatcctcc aatacttgat gtctaataat taaattctct gtcccaaggc
     4621 caaggttggt cagcccattg ccaagggcat ctacttggag catacccaaa gttagttaaa
     4681 ggaccgtatt tattttcgta agcatatcta gcttgcgcaa attgattaca taatgaatta
     4741 taagtgttga tagcattttt atcatcaggg tgattgtcta aatataagtt catatcaaca
     4801 catgcaaaag atgtttcctg aacttttctc ataagttcag ctctagatga gtctttcatt
     4861 atcttctacc tcctctatat ttttttggct tagcatataa ttctttgttg tacatttcag
     4921 actctagtaa gtttagttct ggaaatatag ttccaaattt taatgcatca gaaaggcagt
     4981 acatttctgt gaaaatttga ttattaatgt atggtctagc caaagatgga ccacaatttt
     5041 ttttgatatc tctagtctta ttttccaaga tgaaaacctc cttgttgtaa atagataggt
     5101 aactctatct agtgtataat attcaatcat ttaaaaatcg ttactaataa taaaatatta
     5161 aatgagaaaa ataaaaaaga taataaaaga aagaggttat ttatgttatt ataagtttat
     5221 gtgttattgt aaactataaa ttatttagta aaattaaagt aaaaattata gtattttgtg
     5281 gatgaaggta gacttttttg taaaatatta ttaacataaa tatacataaa tatacgaggt
     5341 gattagatga gtgaactatc aggaaaaggc tgtgaaatta tagttccttt tgaggaaaga
     5401 cttccagtaa gagatataga aaaaagtata ataaagaaat atagaaaaaa tttatggtct
     5461 aagtttatga agtcaataag agattataaa ttggttgaag aaggggataa gatagctgta
     5521 gcgatttctg gtggaaaaga cagtattctt atggctaaaa tgtttcaaga gttaaaaaaa
     5581 catggacaag ttaattttga tgttgaattt attgctatgg accctggata ccatgcaaat
     5641 ataagacaat tacttataga taattgtgag tatctaaata taccaataca tttatttgat
     5701 tcaagaatat ttgagatagc agatgaaata gcaaaagatt atccatgcta tatgtgtgca
     5761 agaatgagga gaggagcatt atattcaaag gcagaagaat tagggtgtaa caaacttgca
     5821 ttaggtcatc attatgatga tgtaatagag acaacaatgc ttaacttatt gtgtgctgga
     5881 aattttaaaa caatgcttcc taaacttaat tctactaatt ttgaaggtat aaaaattata
     5941 agaccacttt attatataag agaagaacat attataagat ttattcaaaa tagtggtata
     6001 tggccattaa attgtgcatg tatggttgct gctaaaaaaa caggtaataa aagatatgag
     6061 ataaaagact taataaagag cttagaatca aattttaaaa atgtagaaaa atctatattt
     6121 aaggcagcag aaaatgtaaa tcttgattca gtacttggtt ggcaaaaaga tggggagaaa
     6181 cattctttct tagaaaattt tgaataaaaa tagttaaaaa gaatcactat aaacaattgg
     6241 aaagggtgtt tatattgaaa agagtatata tagattttga aatgaatatg ccaaattcta
     6301 aatcaaaaag agcagtattt aattctgata taattgcaat cggagcagtt atgtatgatg
     6361 aaaaaacaaa aaatattgat aaatttaaat ctttaataag accagtaagt aatgaggagt
     6421 tatatcctca tatacaagaa ttaacacata tatcttctga agaattgaag agtgcaccaa
     6481 gttatgaaga ggtcatgaga aaatttaaaa aatggcttgg catattttct gatattaaag
     6541 gaatatatac ttttggtaat ttggatttga catgtttcaa taacacagat atgagaagtg
     6601 ctaaaaaaaa taaccatcca aggtttgtaa ataatattag agatttattt gtagatataa
     6661 aagaaaaata tataaattgt ggtatgagat gtatgaacta tatatcattg aaaaatttac
     6721 ttgaatttgt taacttagag tttagtggag atgcccatga cccattaaat gatgcatata
     6781 atttatttat tttggatgag gcaatcacaa ataatgtaga tatacaaaat cttttaataa
     6841 tacgtgacat aataagacca ccatttaacg atataaattc taatttagat aattgtttta
     6901 acaaatttaa ggagtcatta tataaaaaag aaggaaatta taatattgta gatttttctg
     6961 ttgaaataat aaaaactgtg agaatgtact tattaacaat catagatgta aatatacaaa
     7021 atttagaaat tatgaaagat ataaataaaa aaatggatac tatagataaa ttgaaagata
     7081 tagaggaagg ctatttttat ttattagaag atgtgtacct cgacatgaaa gatgtattag
     7141 aagatttaat gctttataga atgaatgaag atgagtacaa gtatgagata aaaaatataa
     7201 taaaaatgtt tgatgaagac ttagataatg aaaaaatata tataaataaa tgtaataact
     7261 taaatgttgt taacaagata taagaaatta caaataatag agtagaactt gacaattata
     7321 acaatttttt ataaaactga atcaagataa aattaaatta atataaaaat aatatataat
     7381 aaaaaatgta gagagatact tactttctac atttttttat tgtatattgc tttaggttta
     7441 aaaatatatt atatacttta taatcaatat attttatcta tattttacac ttataaaata
     7501 tgaaaactac ttattgcgaa agtaattgat ttgttgtatt ataatttaca tgaattatgt
     7561 aggacaatca aaagtcagta ttataagagt acatatattc aaatttttaa caattaattt
     7621 atataattaa aaaatgaagg gtaggatgaa gtcatgggtt ttagagaagt aaaaattgag
     7681 gaactacagt ttaatccatt tactaagatt ggcaaagaat ggttacttat aacagctggt
     7741 gattcagaaa agtttaacac aatgactgct agctggggtg gtgttggagt ctattggggt
     7801 aaaaatgttg ttacaactta cataagacca cagagatata caaaagaatt tgtagatagt
     7861 aatgatacat ttacagttgc cttctttgat gaaacgtata gagaggcttt aaatatttgt
     7921 ggtactattt ctggtagaga tataaataaa atagaaaaag caggacttac accatatttt
     7981 gtggatgata cggtagcttt tgaagaagct aatatgatta tagtatgtaa aaagctatat
     8041 catggtaata tgccaccaga aaactttgat gctaaagaaa atgataaaaa atggtatcca
     8101 gagaaagact atcatactat gtacatttca gaaattataa aagttcttgt aaaagaataa
     8161 ttataataaa aaatatatga aaagaactta ttataagtaa atgactgtgc tttcttatct
     8221 gatatctaag tagatgctac taactaaata aatattaatt ttaaataaat actattataa
     8281 aaaagatgaa ctatatttac tatagttcat ctttttattt atataagcac ttatataagt
     8341 agagatttat ttgaataaaa gtatttattt gaaatatttg agcatacttt ataagtattt
     8401 ttatgctaac tagaaacact taatttatgg c
//
LOCUS       DAEPOG010000161         8319 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEPOG010000161 DAEPOG010000000
VERSION     DAEPOG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.161"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            341..1501
                     /locus_tag="KR432_003016"
     CDS             341..1501
                     /locus_tag="KR432_003016"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH0641532.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1503..2633
                     /gene="glgD"
                     /locus_tag="KR432_003017"
     CDS             1503..2633
                     /gene="glgD"
                     /locus_tag="KR432_003017"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBH0641533.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2662..4104
                     /gene="glgA"
                     /locus_tag="KR432_003018"
     CDS             2662..4104
                     /gene="glgA"
                     /locus_tag="KR432_003018"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH0641534.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4118..6538
                     /locus_tag="KR432_003019"
     CDS             4118..6538
                     /locus_tag="KR432_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBH0641535.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6543..>8319
                     /locus_tag="KR432_003020"
     CDS             6543..>8319
                     /locus_tag="KR432_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBH0641536.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSL"
BASE COUNT         3330 a          809 c         1554 g         2626 t
ORIGIN      
        1 attttgctat tacgaattta acaaattaaa aaataagcat gtttatatga cacaagggat
       61 ttataaaaaa tgtttttata tttataaaat aaaatttgta ttatttttta accgataatt
      121 gaatattaat aaatactata gtgcttatgg aaaataatgt atacgtcggt aagttcatta
      181 tggggtatac attatatcaa ttgtatagtt gatattattt tatttttatt aaggtattat
      241 ttaagcaaca tttatacacg gcatttattg actgataaag tttataaaag taaaaatgca
      301 attataaata ttaattacta gatgagggga gtgtcattaa atgaaaaaag agatgttagc
      361 tatgattttg gcaggagggc aaggttcaag attaggtgtt tttacaaaaa gaattgccaa
      421 accagctgta tcatttggag gtaagtatag aattatagat tttgtgttaa gtaattgttc
      481 aaactctggt attgatactg taggtgtttt aactcaatat agaccgttga tattaaattc
      541 acatataggc atgggaagtc attgggattt ggacagaata aatggtggtg tctatgtttt
      601 acaaccattt atgaatgaaa aagaaggaaa ttggtataat ggtacagcac atgcgatata
      661 tcaaaatatg gactttgtag atacctataa tccagaatat gtattgatat tatctggtga
      721 ccatatatac aagatggatt atagcaaaat gttaaaattt cataaggaaa aacgctctaa
      781 agctacaata gcagtgattg aagttccttg ggatgaagct tcaagatttg ggataatgaa
      841 tacaaatgaa gattcaagta tctatgaatt tgaagaaaaa ccaagtgaac ctaagagcaa
      901 tttggcatct atgggagtat acatatttga ttggaaaatg ttgagaaatt attttaagga
      961 agcagaaaaa aatccagaaa taaattatga tgattttgga aaaaatttaa tacctaaaat
     1021 gttagaagat aatgttggaa tgtatgctta tccatttaaa ggatactgga gagatgtagg
     1081 aactatacaa agtctatggg atgctaatat ggatataata aaatctccag aaacacttga
     1141 tttagcagac cctaaatgga agatatatac aaatactatg gcaatgcctc cacaatatat
     1201 aggtaaaaat gcaaatgttc atagatcaat gatagctgat ggttgtagaa ttttaggtga
     1261 agtaggcaac tctgtattat cacatggagt tgttgttggt aaaggaagta aagtaattga
     1321 ttcagtaata atgccaaatg ttgtaatagg tgaaaatgta actatagaaa aagctatgat
     1381 aggtgagtgt gcaactataa atgataatgt tcagataaag aatgtaaata atgaaataaa
     1441 tgtagtttct gagtatgaaa atatcgaacc tagatgcgta ttaatagaag gaggtcttta
     1501 agatgagaaa tgaatgttta gggataataa atttaaacaa aaaaggagac ccagcaataa
     1561 acaaacttaa ctatggaaga ccaatagcat ctacaccaat agctggaaga tatagaataa
     1621 tagactttgc tttatctaat atgataaatt caggaatcac taaagttgga atattcgcaa
     1681 aagaaaaata cagatcattg acagaccata taggtagtgg taaagattgg gatttaagta
     1741 gaaaaaaagg tggcctatca atttttagtc cagaaaacac taagtataga aatacttact
     1801 cacatagaga aggtgatata tatactatct tagctaatct agattatata gaaaagagtg
     1861 aagaagaata tattttaata gctccaagct atatgctatg caatttaaat tattctcaag
     1921 cattggaata ccataaaaaa tctcataatg atataactat aatatataaa aatgtaaata
     1981 atgcaaataa agattttgct ggtaacttaa cactaaattt agatagtaac aatagggtaa
     2041 taaatgtagg aaataattta ggcaaattcc ctagagcaaa catatgtatg gagacatata
     2101 ttatgaagcg tgaagatttt gttgaatgta tatataacat tgtaaataaa ggcagttatt
     2161 gttatttaga agaatttatt attgaagaag cagaaaatat gaaaatagga gcttatgaat
     2221 atacaggata tctaaaatgt gtaaattctg ttgaatcata ttttgaaatg agcaaagatt
     2281 tattaaattt agaagttgca gatgaattat tatactctga aagaaagata tttacaaaag
     2341 aaaagaatga atcacctaca atttatacag atagtgctaa agtagaaaat agctttatag
     2401 catctggttg tttaatagaa ggtactgtaa aagatagtat catatttaga aaggtaaatg
     2461 ttgaaaaagg aacagttata gagaactcaa tagtaatgca aaattgtgta ataaagtcaa
     2521 atgcaaaact atataatgca atattagata aaaatacgag tgtatcaaat gggaaagaat
     2581 taagaggaga tgaaaaatat cctcttgtag tggaaaagaa tacaaatata taaataaatt
     2641 ttttagaagg gggaatgact aatgaaggtt ttttatgtaa cagcagagtg ttggcccttt
     2701 gcaaaaactg gaggattagg agatgtttct tatgcattgc ctaaagaatt aaaaaaagaa
     2761 ggtgtagatg taagggtaat aatgcccaaa tattcaacta taccaagtta tttaaaagat
     2821 caactaaaag aaatagctgt gtttagtgta cgtgttggat ggagaaatca atactgtggt
     2881 cttttagaaa tggaattgga tggtgtaaaa ttttacttta tagataatga attttatttt
     2941 agaagagaag atgaaagaaa gagtatctat ggatatggag atgatgcaga aagatatact
     3001 ttctttacag atgcagtttt agaagctata agtagaattg atttttatcc agatgtaata
     3061 catataaatg attggcatac agggatgtta ccattaattc taaaagaaag atatgcaacc
     3121 cttgaggggt ataaaaatat aaaaactatg tatactatac ataatctcca atatcaaggt
     3181 gtatttgata agcatgtttt atatgatata ttggacttac cacaaaaata ctttgataat
     3241 agagatatag agtattatgg ctcaataaat tttatgaaag ctggtataaa ttttgcagat
     3301 aaaattataa ctgttagtcc tacttatgca aatgaaatac aaacatcttt ttatggtgaa
     3361 caattagatg gattacttag aaaagagtca ggaaaattaa aaggtatatt aaatggtata
     3421 gattatgatt taaacgaccc tgcaaaagat aaagatatat ttgttcatta tgatgtagac
     3481 tcaattgaca aaaaagtaga aaataaattg agattacaag atatattggg acttaaaaaa
     3541 gattcatcta ttccgttgat aggtatagtt tcgagattag tttctcaaaa aggatttgat
     3601 ttaatagcat acatgatgcc agaacttatg agagaagatt tacagattgt cgttttagga
     3661 actggagaac atcaatatca atcaatgttc aactattacg attctaattt ttcagataaa
     3721 gtatcagcta gaattacttt taatgcatca ttggctcaac agatatatgc tgcaagtgat
     3781 atgttcttaa tgccatcatt atttgagcct tgtggtatag gtcaaatgtt agctatgaga
     3841 tatggttctt taccaatagt tagagagact ggagggctta gagatacagt aacaccttat
     3901 aataaattta ctggagaagg caatggattt agttttaaaa attacaatgc tcatgagatg
     3961 tttttctgtt tgaaaaatgc aatcaaagta tttaaggaca aagaaaaatg gattaaatta
     4021 gttgaaaatg caatgaaaac agataatagt tggaaaaaat cagctaagga gtatatagaa
     4081 acatatagag acatatgtga ttaaaggagt ggtttttttg gtaacaataa gcaaaaagaa
     4141 attcaagcaa aaatttgaag taaaaatgta tagcttatat gcacaatcta tcaaggaagc
     4201 tactgatgaa caattgttaa atgtattatg tagtctattg aaagatgaga tagcaaaaaa
     4261 atgggttgct acaaagttag ataagaaaaa agaagtttat tattttagtt tagaattttt
     4321 aatagggaga cagttaaaat ctaatttatt aaatttaaat attgaagaag aagttagaga
     4381 aggtttatct gaattaggaa tcaacttgga tgatttaatt gaagctgaag tagacccagc
     4441 cttaggaaat ggtggacttg gtagattagc agcttgcttt ttagattcta tggcatcttt
     4501 gaacataagt ggtcaaggat atggaataag gtataaatat ggtctgtttg aacaaaaatt
     4561 tgtaaatggt tatcaggtag aagtgcctga taactggctt acagaaggac gatatgcatg
     4621 ggaaactgta agaccaaatg aagcaacaat ggtgaagttt ggtggtgagg tagaattaat
     4681 caaggaaggt agtcatttaa aagtaattca taaaaactac cttcctgtaa tggcaatgcc
     4741 ttatgatata ccaataatcg gataccaaaa ccaatgtata aatactttaa gattgtttaa
     4801 gagtgaaata ccaaagagag attttggtga acttacctca aatgctctaa attattctgg
     4861 tagctatgaa gaggctttaa aacataagta ttatactgag gaaatatcac aggtattata
     4921 tcctgatgat tcaaactatg caggtaagtt gttgaggttg aaacaagaat acttttttgt
     4981 aagtgcaggg atacaagata taataagaaa atataaaaag aacaaattaa atattgataa
     5041 tttattcgat aaggtagcaa ttcatataaa tgatacacat cctaccttat gcataccaga
     5101 acttatgaga attttgcttg atgaagaaaa tttatcttgg gatgaagcat gggaaataac
     5161 taaaaaaaca gtgtcctata ctaatcatac tataatgtca gaagcaatgg aaaaatggcc
     5221 tgtaagtatg atgaaagaac tattaccaag aatttatatg ataatagaag aaataaatag
     5281 aagatatgta gaggaattaa ataataaagg atatgagcag gatagaataa aaagaatgag
     5341 tataatagac tgtgacaata tcaatatggc aaacttatgt attgtcacaa gtcatagtgt
     5401 aaatggagtt gctaagcttc atactcatat tcttgaaaca gaggtattaa aagatttcta
     5461 tcaagatgaa ccaaataaat ttaataataa gactaatgga attgcacata ggcgatggct
     5521 tataagttca aatcctcaac ttagcaattt aattacagat ttaattggtg attcatggaa
     5581 gaaggatacc ttacaattaa aaaatattga aaaatttaaa aatgattcct ctgttttaca
     5641 aagacttgat gatataaaat acaataataa agcaaatcta gcaaagttta taaaagataa
     5701 atacgattta aatgtagacc caagttcgat atttgatgtt caagttaaga gattgcatgc
     5761 gtataaaaga cagttactga atatatttaa tgtacttcat atgtatcatg aattacttga
     5821 taatcctaat ttgaatcttg acccaagaac ttttatattt ggtgcaaaag cagcaccagg
     5881 atattatttg gctaaatgta taattaaatt tataaattct gttgcaagta ctataaataa
     5941 tgatgttaga gtaaaggaca agttaaaagt agtattttta gagaattatg gagtatcatt
     6001 agcagaaata ataataccag cagcaaatgt tagtgagcaa atttcaacta caactaagga
     6061 agcatctgga acaagtaata tgaagtttat gatgaatggt gctataactt tggctacact
     6121 tgacggcgca aatgtagaga tatgtgaaca ggttggtaaa gaaaatatgt ttttatttgg
     6181 acttagtgca gaacaagtac taaattataa taaatatggt ggatattctt cacttgactt
     6241 atatcattct aatatggata taaaaagggt ggtagatgat ttaataaatg gatttattcc
     6301 taatcttggc gaagaaggaa gaagtattta taattcactc actacttaca atgatgagta
     6361 ttttgtattg agagattttg aaaattatgg tcaagctcaa gcagatataa acaggttata
     6421 tagagataaa gaaaaatgga ataaaatgtc tttagttaat atagctaact ctggattttt
     6481 ttcttctgat agaacaattt ctgagtatgc aaaagacatt tggtttaaaa gggtgtgatt
     6541 aaatgcaaag tgtaatagaa tataactctt gggataaaaa ttttaaggct ccgtttggag
     6601 ctttaaaatt tgatgaggaa ttgaccattt gtgttaaagt aaatgaggga tacaatatca
     6661 agtctatttc cctagaaata aatagagaag aagaaatgag aacaataact ttaaatgaag
     6721 aattagataa tgataaactt ggaaagtgct tttgtggtaa gattgaaaag tttgatggta
     6781 caggtgttta tttttattac tttaaagttg atgttgaaat ggatggtcag ataaagactt
     6841 tattttatgg aaaaaacaga gataatggat attcttgtga atataattat agtgatatta
     6901 ataaatatca aatcactgtt tacaaagatt ttaaagtacc aacatggtac aaagaaggaa
     6961 tactttatca tatatttgta gatagattca ataatggaaa tcgtagtggt aaggttgata
     7021 atcctaaaaa aaatagtttc atatatggaa attgggaaga tatacctatg tacataaagg
     7081 atagtcaagg ggatgttata agatgggatt tccatggtgg aaatttaaga gggataatca
     7141 ataaacttgg atatttaaaa aaattaggtg taagtatact atatctaagc cctatatttg
     7201 aagcttctag taatcataaa tatgacacag gtgactataa aaaaattgac ccaatgtttg
     7261 gggatgaaga tacatttaaa gaacttattg ataaagcaaa agaaaaaggt atatcaatag
     7321 tattagatgg agtttttagt catactggag cagatagtaa atattttaat atgtatggaa
     7381 actataatag tttaggggca tatcaatcta aagaatctcc atattattca tggtatatgt
     7441 ttgaagaatt tcctcaaaag tataaaagtt ggtgggatgt taagacattg ccaaatataa
     7501 atgaattaga acatagctat atggattata taatatatga caatgatagc gttataaata
     7561 aatgggtaaa tatgggtatt aagggctgga gactagatgt tgcagacgaa ttaccaacta
     7621 agtttataag agagttaaaa aaggaattaa aaaaggcaga tgatgactca attcttatag
     7681 gtgaagtatg ggaagatgca tcaaataaga ttagctatgg tcagagaaga agttatttat
     7741 taggtgaaga attagattct gttatgggtt atccttttag aaataacatg ttttctttct
     7801 taaaaggaga aattaattca tatgaacttt gtaatagata tatgcagatt aaagaaaatt
     7861 atccaaaaga atcatttagg tctaatttaa atttaattgg aacacatgat gttacaagag
     7921 ctaaaacgga attaaatgat gatgtagatt tagtgaagct tgctgtagct atacagatga
     7981 catttgaagg agtaccatat atatattatg gagatgaagc aggtctttgt ggaggtgttg
     8041 acccagataa tagaagaact tatccatgga aaaatgaaga cgaagatatg cttaattttt
     8101 atagagatgt tataaaaatt agaaataaga ataaactttt aagttctgga aatacagaat
     8161 ttatatatac taataatgat tctgtatttt cttttattag agttgatgaa aataatgata
     8221 gaatgttaat tttagtaaat agaagtgaaa atgtggaaag tatatcatta tgtatagaga
     8281 gtagttttat tgaggaaata cctataaagt atagtctta
//
LOCUS       DAEPOG010000162         8260 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEPOG010000162 DAEPOG010000000
VERSION     DAEPOG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.162"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            19..687
                     /locus_tag="KR432_003021"
     CDS             19..687
                     /locus_tag="KR432_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641537.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            846..1352
                     /locus_tag="KR432_003022"
     CDS             846..1352
                     /locus_tag="KR432_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0641538.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1480..2496
                     /locus_tag="KR432_003023"
     CDS             1480..2496
                     /locus_tag="KR432_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0641539.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2850..3554
                     /locus_tag="KR432_003024"
     CDS             2850..3554
                     /locus_tag="KR432_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBH0641540.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3849..4328
                     /locus_tag="KR432_003025"
     CDS             3849..4328
                     /locus_tag="KR432_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0641541.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
     gene            4967..5344
                     /locus_tag="KR432_003026"
     CDS             4967..5344
                     /locus_tag="KR432_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641542.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            5337..6371
                     /locus_tag="KR432_003027"
     CDS             5337..6371
                     /locus_tag="KR432_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0641543.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            6437..7024
                     /locus_tag="KR432_003028"
     CDS             6437..7024
                     /locus_tag="KR432_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0641544.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(7402..8163)
                     /locus_tag="KR432_003029"
                     /pseudo
     CDS             complement(7402..8163)
                     /locus_tag="KR432_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3310 a          764 c         1293 g         2893 t
ORIGIN      
        1 tttaaagggt gattttttat ggaaaatata actagttatt ttacaacaat actatgtgta
       61 tttatatgct tatcatctgt atttatattt actcaactag ctagagtatt tattaataag
      121 aagaagatta atcaaaaaat taaatcaaga aacggattta ggtatgatag agattttatt
      181 gaagccagaa gagaagaaat acatataaag gataacaata ataataaaaa taagagtaac
      241 aataagaaat taaaggaaga aaaagtattt aaatacgata atggtgattt atataaaggt
      301 gagtttgttg atggaaagaa aaatggattt ggaatatata ttttttcaag taaggaaaag
      361 tatgaaggat tatggaaaga tgacaagatg catggaatag gaaaatatac ttatagagat
      421 ggttcaatat atacaggtga atttaaatac ggattaaaaa atggtttggg aaaattaaca
      481 tatcccaata atgacattta taagggatat tttttagata ataagaaaaa tggtaagggt
      541 gtattatata aaaatgatgg aaataaacaa gcaggaatat gggaaaatga tgaacaatgt
      601 aaaagtttag attttaaaga tttaaataat aataaaataa ataatgttta tcaaaataga
      661 ttaaataaca ataaattaaa agtttaatta ttctaataaa aaaatcttta tttatgatgg
      721 aatataggat atatctatgt tgtaatttgt aaaatcttcc tatatcatat aaaattaagt
      781 attgattagt ctattacata aatatagaat ataaatatat ccatttaata ataaggagaa
      841 gagatatgaa agaatatgta gttgtggatt tagaaactac aggattagat ccatataaag
      901 gttgtgaaat aatagaaata ggaataacag aaataaaaaa tgaacaaatt gtaaaaaact
      961 attcaaggtt gataaaacct aaggggataa tttcttcatt tataacagaa ttgacacata
     1021 taagtaatga aatggtagaa aatgaagaac cactagaatt agttttacct agatttagaa
     1081 aatacatagg tgatagaact ataattgcac acaatgcgaa atttgattta aaatttttaa
     1141 attactatct taggatgtta gatttagaac caataaacaa ttatatatgt acagtagaat
     1201 tattaaaaaa atgtaaaagt tataaaggaa aaaacaagaa acttgaaaca gcttgtgctt
     1261 attacaacat agagaatata aatgctcata gagctgacag tgatacatta gcaactgcaa
     1321 aactattttt gaaaataaaa gatgaatatt aattataaat atagagaaaa ttagtgaaaa
     1381 tatcctttat aaaattgacc cttaagctta caattttgtt ctttgattta ttttgtagaa
     1441 caatgtataa tgtaccttat atgaagaagg gagactaaaa tgggttatta caatgataat
     1501 gataataata aaataagacg taaaaaaatt agtaacaaca aagatagtaa taaaaaatct
     1561 aaacattctt atacttcggc aaataaaaaa aataaaagtc ctaatacaag ttccagtaaa
     1621 agaatgagtg aaaaagtaga ttcacagaat cttataaaaa gagatagaga tagttttaca
     1681 agagataatg taagaccttt aaatacagat tacaatcaaa gagaaagaag aagagtcgag
     1741 tataaacaaa gagagaaaaa attaaaaagt aaaaaaagaa aaaaacaaaa aaggattaga
     1801 atagttaggg cattattatc tattatgtta gttgcactaa ttatattttc agtggcaggt
     1861 gcaatattta cagtttcagc tataaaaggg tcaccagaag ttactaaaaa gttaattagg
     1921 tctaaatata taagtagtga ggttgtaagt ataaaacaaa tgccaaatga tttaaaacat
     1981 gcaatagttt ccatagaaga tgagagattt tataagcata aaggagtaga tgttatttct
     2041 ctagttaggt cagtattaaa caatgtgttt acagatacaa cacaaggtgc aagtactata
     2101 gatatgcaag tttcaaaaaa cctgcttact agtaatgata aaactatgaa gcggaaaata
     2161 agggatatgt ataatgctaa gcagatgaat aagattatga ctaaggatga aattcttgag
     2221 gcatatttaa ataatatgta tctaggtaaa aatgcatatg gagctgcaaa gggagctgaa
     2281 gtctactttg gtaaaaaagt tagtgaatta aatttagctg agtgtgctat gttagctggt
     2341 atgacaaata atcctgcaag gtatattgac catggagagg ctaagaagag acaagtgatt
     2401 gtactgtata agatgcatca gcttggttat ataactgata aggagtatag aatagcaaaa
     2461 gctgatgaca ctccatttaa atcagaaatt gattaaaatt gtaagaatat agtattttca
     2521 atagtttaaa caaacaaaaa tgtaataaaa aaggtgattt gccacccatt tgccaccata
     2581 attataatct aatggcaaat tgttttaatg tacaaactag ataatagaat attgttttaa
     2641 atgctatatt atgctaaaat ttaattgtat tttgtaaatt aaaaaactta taatttagtt
     2701 ttctagggtt ccgcatttta taatggtcag gtccaagaga aaacgcgcaa ttgttgtgta
     2761 cacggaagga taaaagcctg ggagatattt aataatattt ctataggctt ttttattttt
     2821 acatatttta aatttgagga ggttttctaa tgaaaaaatt tgttattgag gatgcatttt
     2881 ggaatctatt tcctagtgca aaaattggag ttattatttg taatgatata gataactcta
     2941 taaaagatga agactataag aaaataattt tgaagggaga agaagaagca ttaaaatact
     3001 tggaggactc agaatttagc aacaaccaag tgattaaagt atggagagag gcatttaaaa
     3061 aatttaaaac gaaaaaagga gcaaggtctt ctattgaagc attattaaaa agagttcata
     3121 atggaaataa tataggaact attaaccctt tagtggatat ctacaatttt atctcattaa
     3181 aatatgcatt accttgtgga ggggaggata tagataaatt tataggagat attaggctaa
     3241 ctagagcaat tggtaatgag gagtttatcc cactagggac agatgaaaat tcatttccat
     3301 atgaaggaga aataatttat aaagatgatg gtggagcaat ttgtagatgt tggaattggc
     3361 gtgaagcagt gagaacaatg cttactgaag atactaacaa tgccttttta tgcattgaat
     3421 tagtagatga aagtagattt aaagaatttg aaaatgcttt aaatgaatta gctaaaatag
     3481 ttcaagaaaa attgggtggg aattgtaaaa tttcaatttt agatattgat aataaagagg
     3541 tatcagtgga ttaatctgga gatgctgttg taatggacta ttctagttat aaagatactt
     3601 atatttaggg taataagttc tattttagat gtaacgatta tcaaaggata taatatagaa
     3661 aataaaaatg tagaatataa aaagccttta aaataagaaa aaaatgtatt ttaaaggctt
     3721 ttattatact cttttttatt tgcataatta acaataaaaa tatattgtta attataatat
     3781 ttttattgaa aaacgatata atataatgta ttatactata tataaaatat tttaaaggag
     3841 ttggtatgat gcctaataaa cttataaaaa atttactaag tggtatatta caaattttat
     3901 tttttctttt agggttagta attgttgtgg gaggatttaa aagttttatg tacctctgtt
     3961 ttagtggaga agcaactcta caaggcacta tttctggaat attaatgttt attttaggtg
     4021 tatcatattt tatcattata aaatcattga tagaagtatt aagtagttct gaacatagct
     4081 tatttgtaaa agataatgta aaaagattta ggattattgg atatcttttg cttttaaact
     4141 ctataatgga gtttatttca acttttggta ctactggaaa aggaatgaga ttcctagatt
     4201 taggatttgg attttatttt actgttccag tatttgtata ttttataact tctcttatga
     4261 gttttgtaat tgcagatgga tttgtaaagg caataaagat aaaagaggat aatgatttaa
     4321 ctatttaggt gtgacacttt aatatatagg agataaataa ccagaaaaga gtgattaact
     4381 agtaatttaa aataaaatat attagggtat aatacataaa ctataatgct aaaattaaaa
     4441 gattataata ctaaaatata attatgtttt aattaaaata aagaacttta ttagtagata
     4501 ttaaaagcac atttttttat aaaagtgctt ttttataatt tattaaaggt ataattataa
     4561 aagtcttatt caataaaatt tataattaat ttagatatat ttgtaaataa tcagtataaa
     4621 atagatgttt taatagcata aaggcataat ttataaggtt attaaaattt attagatatt
     4681 actctagttt attttaaaaa aaagtattat aatattcctt gtgtgaaaat ataggaattt
     4741 ttattaaata gttttaatgt gtaaataaca ttctacacta ttgtaattaa atatgtaaaa
     4801 agtaaaatat ttaaatagat atattttaaa atctaacttt taatattgta aaaaaattag
     4861 aaatttaaca aaaagtacaa aaattcacat ttttttcata aagtgtttga ctaaaaaatt
     4921 cacatgttgt agaattaagg tagcaggagt tttgagggag gtgtaaataa tgataagaaa
     4981 aataccacaa gcagaactaa aagtaatgaa attcatttgg aaagtagatg ttacagtgac
     5041 atcaaaagat gttattgaag ctatggaaca aaaatatggc tggaaacaaa ctactacact
     5101 gacactttta tctaggctgg taaaaagagg attcttagat gctcaaaaaa tagacagata
     5161 tacacattac acaataagag taaaacacaa agattattta agttttgaga caaaggattt
     5221 cctaagtaat atacatgatg actctttgag aagcttaatt tcagcattac atgatgatga
     5281 aaatataggt aaagataagc tagattttct agaaagttat tttgataact taaaggatga
     5341 ataattatat gctatatgtt tttgaaaatc ttataaagac aactattgta tgtagtcttg
     5401 gtatttgtct attattattt ttgaaaagat atttatttaa gaagtttagt aagagattca
     5461 attattatat ttggttgata atagtcttta gaatgttact ttttctattt aattatacaa
     5521 tagtgtatga agtaaaagaa cccaaagaaa atgcagtagg aaataatatt acacaaattg
     5581 atatttctac agataataat cttatgttgt atgtagctta tttatggtta gttgtaacta
     5641 tagtcattgc agtttataca tttataaagt atacaagatt taaaaatcta gttgttgatg
     5701 tatcttatga tattgaagat aatgatgtaa actgtatcta caaaaacttg ttgaaagaat
     5761 taaatataaa aaagaaaatt gaattaagag gttctgatga attgataagc ccagcaggca
     5821 tgggattatt taaatcttac atattcttac cagactatcc ttatagtaaa gatgaactaa
     5881 cttggatttt gaaacatgaa ttaatgcatt ttaaaaataa agatatttta ataaagtttt
     5941 tagtgttatc tgttagaata atatattggt ttaatccact tgtttatgta atgagcaata
     6001 aagtaaatct agattgtgaa ttatgttgtg atgaaagtgt tttgactgat tgttctttaa
     6061 aggataagaa ggagtatgca ttagcactta taaagtcaat taaacttagt aagaattata
     6121 atagtggtat tttaacaaca gagtttaata agacaagtct ggaaaagaga cttgacagta
     6181 ttgttaagaa aaaaggtaaa aatggaatat tggtgttatt gattttgatt acattatttt
     6241 cagttacata ttttaatttt gaaagtatta gttttgataa ttcaaattct ttaaggaata
     6301 tggggatttt acaaaaatca ggagtagatc ttaaggaaga agatgatatt agcatgagag
     6361 ttataaatta atagattaat gtatgtaatg aaaaggtata ttgtcgtatt attaataaat
     6421 taggaggaat tatgaaatga aaaatttaaa aaaaatttta ataattacag gttgtctaac
     6481 tctaatatct actagtttgg tatttgcaaa tactactact gatgtagata catcaaagga
     6541 tgatgattca ataataagtt catgtactat aactggttat gaatttacat atgaaaacgc
     6601 accacaatct gtaaaatttg attatgaaaa aaaatgtgaa gcgctaaata taaaaccatc
     6661 taaaaatgat ataatttttg ttaacaaaga ttctatagat gcaaatgatg tagaaaacta
     6721 tgttgagcca aaagcatata ttattagata taaaaaaggt tatgtttttg aagttactgg
     6781 acctaagagt tatacattaa atgtaagtaa tactgtagtc ggatataatc gtgttgaatc
     6841 aggtaatcct gtgcatttag ttcaactttt atgtagaacc ttagatggtt caagtattga
     6901 tgatgatagt gaatttggac ctgctacata taatgcagtt aagtcacttc aaggaagact
     6961 tggattaaca agagatggaa ttgttggaag aagtacttgg gatgcagctg gaagacgtct
     7021 ttaaaaagaa agctattgca cagataattt taaattgctg aatctgtaaa catttacatg
     7081 tattctttaa ataaaaattt attttttaag tgataattaa atttttacta atagggctat
     7141 ggctttaaaa atcatagcct tattttgata tcttttccat ttttgcaaaa gctattaacc
     7201 ctttaagaaa atttcttgtt tttttgaaaa atttctttct tcaaaataaa tacttaaatg
     7261 agctaaaatt tgagcacaat ttctaatttt ctgagtccat ttttaagaaa tatatatggt
     7321 tgttaagtat agtgatttgt gcaaataatt atccaaagta aaaatagatt tcaatttagt
     7381 aacttttctt aataatctat tctaacttcc cattgaattt gtagtatata tcgatgtatt
     7441 tatttcttga gagtacttaa aatatgttac taattcattc caattgtttc accaacctct
     7501 taatactatt gtatattgat tgtactattt tttctctagt gatagctcac ttaatgctac
     7561 ctcttctgta attgtagcat atctttcttt aaatctttta cagattcttt tctattttta
     7621 tatgatacat aatttaaagt atttttaatt tgatgcacaa catatctttg tacttaagta
     7681 tcaggaaata tcgctttaat tgcattagaa aattcaacta gtctatctat agaagctgct
     7741 attaatacat ctttaattcc catgtttcta gcatctataa gcacttttag ccaatacttt
     7801 gaaatttcat tttctccaat ccatattctt aaaacctctt ttatgctaca tatagctata
     7861 tagactgttt tgtttattat tcgacctcac ttctaacttt ataatgtata gcatctataa
     7921 atacaattga ataaattata ttaagaggtc taagttacca tttatgagtt aaaggaataa
     7981 ctttatcagt agttttagtt attagtgaat gagataatat caattccata agtactttta
     8041 agatgactta atatattttg agtagacatg ccttttgagt atattccaat cacttgattt
     8101 tttattccta atatatcatt ttctatcata acctaaaagt tcattaagct ccgcttctaa
     8161 catttaatgg attgtacttt caaataaatc ttttagtata tcttgaatat ctgtaattgt
     8221 tttggcatca tattcttcaa taaatttttt taatatttta
//
LOCUS       DAEPOG010000163         8125 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEPOG010000163 DAEPOG010000000
VERSION     DAEPOG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.163"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            102..2459
                     /locus_tag="KR432_003030"
     CDS             102..2459
                     /locus_tag="KR432_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBH0641545.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2545..2862)
                     /locus_tag="KR432_003031"
     CDS             complement(2545..2862)
                     /locus_tag="KR432_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH0641546.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            3201..3956
                     /locus_tag="KR432_003032"
     CDS             3201..3956
                     /locus_tag="KR432_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBH0641547.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
     gene            complement(4595..5866)
                     /locus_tag="KR432_003033"
     CDS             complement(4595..5866)
                     /locus_tag="KR432_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0641548.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   6513..7467
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6513..6541
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            7876..>8125
                     /locus_tag="KR432_003034"
     CDS             7876..>8125
                     /locus_tag="KR432_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641549.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLP"
BASE COUNT         3098 a          864 c         1172 g         2991 t
ORIGIN      
        1 tattatataa actatagaat aataaaatat aaaaattgag aattttataa tatatttgta
       61 gatttgagta taaaaaagtg ctaagttgaa aggagtaaaa tatggataag gttaatttag
      121 aaaggtatag ttgtgatata aatgttggtt taaataaaaa tcaacttcaa agtagaatag
      181 atgataattt aattaatgca tttgataatg gaaaaaccaa aacatataaa caaattttta
      241 aagacaatat atttacactt tttaatctca taaatattgt tttagtctgt ttattagttt
      301 cagttggttc ttttaaaaat accttattta tatttattgc tgtaattaat actattatag
      361 gagtaataca agagataaga gctaaaagat tattggacaa tctatctgtt atagtttcaa
      421 ataaggtatc tgtcataaga gaaaaagaga aaaaagagat tgatgtacaa gaattagtat
      481 tagatgatat tatgatttta aaaacaggta atcaaatttg tgcagattca aaagttttaa
      541 atggaaaatt agaggtaaat gagtctttgg ttacaggtga atctgatatt attataaaaa
      601 ataaagacga ttttttatat tctggaagtt ttgtagtttc tggagaagct tttgtaagag
      661 ttgaaaatat aggaaaagat aactatgcaa ataagatagt taatgatgca aagatttcta
      721 aaaaacataa ttctcaactt agaaattcat taaattcaat attaaaaatt gtaggtataa
      781 taataattcc tttaggaatt atattgtttg caaaacagca ttttggaaat ggagattcta
      841 tagctacatc tgtagtaggt acagtggcag ctgtaaatgg tatgatacca gagggattaa
      901 cattacttac gagcattgct ctagctgtag gaactataaa acttgctagt cataaaactt
      961 tagtgcaaga actgtactgt gtagagactc ttgctagagt tgatacacta tgtcttgata
     1021 agacaggtac aattacagaa ggtaaaatgc aagttgatga tatagtaatg ttggaagaag
     1081 tggatattaa tgaaataatg gggaatatat gtaattctct caaagatgat aatgcaacac
     1141 taaatgcaat aagagataag tataatgttt tagatacgta taaagctaag aatttaatac
     1201 cattttcttc agaaagaaaa tacagtgggg ttacctttga aaataaaggt acatatttat
     1261 taggtgcatt tgagtttata tttaaggaaa aaaataaaaa aattagaata gaagttgaaa
     1321 aatattctaa aaaaggaaac agagtaatag ttttagctca tagtgattct tatatggaag
     1381 ataataatat tcctgaagat ataaagccac tagcttttat attgatatta gataaaatta
     1441 gagatgaagc taaggaaact ttagaatttt tttacaatga aggtgtagat ataaaaatta
     1501 tatctggtga caatcctata acagtaagtg aggtagctag aaaagcaggt cttaaaacgg
     1561 ctagtaattt tattgatgca acaacactta aaaatgataa cgatatatac gaagcagttg
     1621 ataaatacag tgtttttgga agggtaagcc cacaacagaa aaaacaaatg attttagcat
     1681 taaaaaaaca aaatcataca gtagctatga ctggagatgg tgtaaatgat gtgctagcgc
     1741 ttaaagaagc agattgcagc attgccatgg catctggaag tgatgttact aagaatgttt
     1801 ctaatttagt cttattagat tcaaactttg catcaatgcc aaaggttgtt atggaaggac
     1861 gaaaggtaat taataacatt caaagagcat cttctttgtt tttagtaaag acaatttttt
     1921 cattcttctt gtcactatta acattattct tcttcagtag acagtatcca tttgttccta
     1981 tacaattatc actaattgga gctgtaacag tgggtatacc atcattcttc ttagctcttg
     2041 aagcaaacaa aagtagaatt tcaggaaact ttttactcaa tatattaaaa aatgctttac
     2101 caggagcact atgtgtgaca gtaaatgtaa ttattgttta caatatagtt gaatatttag
     2161 gctatagttc aagtgtattt tctacaatgt gtgctatatt aactggtgta tgtggtcttt
     2221 taatattaag acaacaatgt ttgccattta ataaagaaag attattttta attgtaagta
     2281 tgactattgc atttgttatt gggattctat ttttaggtgg attattctca tttgtagcat
     2341 tgagtagaga attaattctc ataactatgg cattagcaat attaatgcca attatgataa
     2401 aagttatgtt atttgtcttg gatgagatat tagagacaat tgttccagat ttaaattaat
     2461 tttaaaatga tataaataag agcagtttta gttgaactgc tctttttaga tgtgtttaat
     2521 tttatataag aatatgtatt tttattaatc agctatatat gagcaacagt agtcaacaac
     2581 ttcattaacc tttaaatcaa aacttgaatc actaggaaca ttaaattttt gcccttcttt
     2641 ataagttaca aactcattgc ttccaggcag ttttacatcc atacttccag caagcatctc
     2701 cattatttct tcttctctag tagaaaaagt atattctcct ggcaacatta atcctaaagt
     2761 ttttctttct ccattaggca gtattattac tctgctactt accttaccat caaaataaac
     2821 gtttgccttc tttactgcag taacattctt aaattcactc attagataaa acctccaaaa
     2881 ttatttgtac atatatatta tataaaaata atgtaaaaaa tgctatatat ttgcaaaaaa
     2941 aaatttagta tttattaaaa aaattaataa gaataaaaaa gagatgaaat tgcttaaatt
     3001 gataaaaaat ataaaataaa ttgcaaagta agcaagaaaa gaataagttg aagagttata
     3061 tattataaat attaaaaatt ttagtattaa taaaaaagat attacattaa tatgaaaata
     3121 taggagatta gaagtgtttg ataattacag tttatatatt ataataaaat aaattgttaa
     3181 ttttaaaaga ggtgaatttt atggcacata tatgctttga ggatatttgt tatttgttat
     3241 taaaagaagc aatgaagtta aagttaatac cacataataa aattgtatta tttgaagatg
     3301 atttatcaat aggtaatatt tttgatgtaa ataactacga attaagagaa aatattttta
     3361 agattagaaa tgtttttgaa aataatccga aactattatt caagccagat ttgttagaag
     3421 ataggaaatt atcaaaatgt agaaatataa taatatggtg tgcaaataac ccaaaagatt
     3481 tgtgtggagt atattatgta gtgagtaagt ttcaagataa aaatataaag attgcattag
     3541 tagatgaaaa aaagacagaa gatgctctaa ttagatattc atttaccagt gaaatgtctc
     3601 cagaagattt tccattcttt ttagataaga cactagaaat tggctttaat gaaaaactag
     3661 aatttaaaaa taaatgggat gtgttagtta aagaaaattc tatttttaga gcattagtag
     3721 gtaacgaaat taaaagtgtt gaagaaactt attttgataa atatatatta agaaatatca
     3781 gtgacaaatg gcaaccttta attgaagtgg ttgcaaactg tatgtttgaa gatgaaatgc
     3841 gtgtaaaaga ttggtttata ctttggagat tagagaagat ggcaagttta aaaatagtaa
     3901 aaataaaagg aagtaaaggg gatttttatt ctgataagga gataagaaaa gtttaaaaat
     3961 tgaatttata ataaattgta ttaaataatg ggtataacta tgaagaaaaa tatcttaaca
     4021 gtattaatat tataattagc aataagtata atatttattc tataatttga aaatataata
     4081 actaatacct tacgaataag tgtgctaaat aaaaaagaaa tacttatttc agggatatta
     4141 gtatatattt taactacatt tttacttaat agtattaaaa tatggtgcta tagagtaaat
     4201 aaactagaaa atttcaactt taatattaca atagtttatt ctaaatgcca cttctttaga
     4261 tataggagat gaaaagcaag ttttaatttt ttttaatcaa tacatgttag atatatcatt
     4321 gtaaattaac ataactttga atataataat gttcagaagg caatattttt gaggtatgaa
     4381 tccaataata tagtcttatg attgtagata tcaaaaaata taagttacta ggaatacgtt
     4441 ttgtagattt atatatgatt aataactaaa gctataaata aacaaaataa aaagagtatg
     4501 tttcaaccta tagcttttag atttaaaata tactctttta ttaattatag ttgcataaat
     4561 taaccataat atataattaa aatttttatt ataattatat attaggttct tcaattagtt
     4621 ttccacatac atatctatga atgatatttc tatcatcacc aacatagata aatctttcaa
     4681 gtctttcaag tatagaataa ttgtcatgat ttaaatttga atcatcaata atcaatgcat
     4741 caaaatcata accttcttca aagctaccaa cttttccaaa gaaacttcca ccacttttgg
     4801 ttgcaatata aaaagcctca gataaagata agaaattata cttcttacca gaattaaccc
     4861 aatataattt agataattgt attgcagata ccattgcttt aaatattgac aatgtatgtc
     4921 caccgcttat atctgatcca agtgctaatc taatattatt atcgattaat tttctgatag
     4981 gcatagcacc actaccaaca ttaaagttag aagctggaca gtgaactgcc ataacattgt
     5041 tttctctcat taaatctatt tcatcctgag aacaatgaac acaatgagcc attaaagtag
     5101 gagtttgacc aaataaatta tatctattat atgcatctcc atagaattta gattcaggtt
     5161 ctagacttct aacccattca atctcatcat aattttcaga taagtgtgat tgcataggaa
     5221 tattatattt taaacataaa tctcctaaac cttttaaaag ttcacttgta cagcttggta
     5281 taaatctagg tgttattatt ggttttacaa tagaatctgt atcactatac ttatttataa
     5341 tttcttctgt atcagcaata gaatcctgag tgttttctaa taaagtatca gggcaattca
     5401 tattcatatt tactttacca acgtatgctc caagaccaac ttctttgaat atatccataa
     5461 gtttcataga ggcttcttta tgtactgttg caaacactgc tattcttgta gttccagatt
     5521 tccaaagttc ttttacaaat tttttataca tttttgttgc atattctata tctttataat
     5581 ttgactcttc tggaaatgta taagtttcaa gccatggcaa aagctcttta tccatagcca
     5641 ttccaagatt tttaaattga gaggcatggg catggaggtc attcatacca ggtattataa
     5701 gcttatcagt gtaatctact acttttaggt ttttgtattc cttaggtatc tctttaaata
     5761 ttccctttac tttacctttt tccagtataa tataactatt ttcaaatact gtgaaagttt
     5821 ctgaagtttt tgtaaaaatt atatttcctt ttaatacttt taacatatag gtctccttag
     5881 ttttaaattt gttttaatat atgtaggtaa aattttgttt tacaaatcaa aattttacca
     5941 catgtattat atcaaatcta aactataaat actattagat aatagcaaat attagtgaaa
     6001 tacagttgtt tactattatt atatataact gacacttaag tgacatttaa gaaaaatata
     6061 atgcttactt acataaaatg gaatgttatt taaagagaac tttgattata ttttcaaaag
     6121 cttttttatc catatcgttt aaaacaagaa aatatctatt catagttatt tttatattag
     6181 tatgtcctaa tctttcagag atgattttta tattagttcc agctagaaga agaattatta
     6241 gaatagataa tatagtaagt atttacaaat atgtaggtgt tcttaaattg ataaattatt
     6301 ccattttaat tttatagttt gaattttatg atataataaa aatatataaa ttttgcagtg
     6361 agcgatattt ttgataaagt agggtttaac agttgcaatg taagggattg agggtgtatg
     6421 ataaatgtta tcaattgcac tactcatggt tcactgcaaa tttgagagag ttgtatgtgt
     6481 gtaagtactg aaaatactta gtttattttg gggttttata ttaactatgt ggtatgtaaa
     6541 tttaatttat ctataattta aaacggtgaa ttacatatgt tttatattaa ctatgtggta
     6601 tgtaaattaa agaggggtac ttcgtacttt taccactcaa actagtttta tattaactat
     6661 gtggtatgta aataggtcaa gcatagattt tgcaactgga agataagaag agttttatat
     6721 taactatgtg gtatgtaaat gttttagaac agaaaaaatt gtataaattg tggaatggtt
     6781 ttatattaac tatgtggtat gtaaatttgt aaaatgtaaa tagcatatta atttttcttt
     6841 cacgttttat attaactatg tggtatgtaa attggagcat tatgttttta ggttacggag
     6901 caacatttag ttttatatta actatgtggt atgtaaatgg tgatagtgga agttttactt
     6961 atacttggcg tgctggtttt atattaacta tgtggtatgt aaatttcaga atacttagct
     7021 cctgcgccta tatcgtctga atgttttata ttaactatgt ggtatgtaaa taaagttttg
     7081 cgcattgtat gtgtgcctag attatctgtt ttatattaac tatgtggtat gtaaatatgt
     7141 ccccacatca attttcctcc gcatgaaaaa atcgttttat attaactatg tggtatgtaa
     7201 attttagaaa caacagaata aacataatat ataagcatag ttttatatta actatgtggt
     7261 atgtaaattt ttacttttcc tttctatgtg tccaattcgg acacattgtt ttatattaac
     7321 tatgaggtat gtaaatctta tacttagtta gaactatata tcgacacaaa tatgctttat
     7381 attaactaca tgatatataa attgcaattt ttattcgttg tccaatcgct tgaaatttgt
     7441 tttatattaa ctacgtggta tgtaaatttg caacaagtat aggtaaaata ccccaataat
     7501 ttatacagca ttttctactt taaaatataa ttatttttta tcatttgtag taaataatta
     7561 ccagataaca ttgactttag ttttaatgat taaaatataa aagtagaata attataaaaa
     7621 gtattgaaaa gtttataaat atatataata aaacttaatg acaagatatt agatataaaa
     7681 aataattatc ttataaatag attgaaattt atgaatattc atactataat ttaaatataa
     7741 agagatgtcc tttaaaaata aaaattaaaa aatatttaat gctactacaa taggaactag
     7801 aactgcactt aataaatata cagaaattag atttggctca atataaaata caaatataga
     7861 atttaggtgt tttttatgaa aaaaatttta tatgctttat atagtttcat tgttattata
     7921 gctaatttta gattaaaaga aaaaaattat aactttattt tattagcaat cttttttata
     7981 ctgctgttaa ttcaaaatgg agttttaaaa acaggttatc aaaatagagc tacattttat
     8041 caagataatt attttaatgg agaggataaa aatgataaga taaaatttat aactataaag
     8101 atttttatat ttttgtcatt accat
//
LOCUS       DAEPOG010000164         8010 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEPOG010000164 DAEPOG010000000
VERSION     DAEPOG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.164"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            15..1811
                     /locus_tag="KR432_003035"
     CDS             15..1811
                     /locus_tag="KR432_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBH0641550.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(1951..3732)
                     /locus_tag="KR432_003036"
     CDS             complement(1951..3732)
                     /locus_tag="KR432_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0641551.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3755..5641)
                     /locus_tag="KR432_003037"
     CDS             complement(3755..5641)
                     /locus_tag="KR432_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH0641552.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5658..6155)
                     /locus_tag="KR432_003038"
     CDS             complement(5658..6155)
                     /locus_tag="KR432_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBH0641553.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(6473..7954)
                     /gene="cls"
                     /locus_tag="KR432_003039"
     CDS             complement(6473..7954)
                     /gene="cls"
                     /locus_tag="KR432_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0641554.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         2768 a         1345 c         1144 g         2753 t
ORIGIN      
        1 agttggaatt ttttatggga atagaatatt ttagtaaact tttagaaagt agtactgaaa
       61 aagaattgct cttaagtaaa aagtcaaata acataagtac agcaagatta atatcttttt
      121 tgatagtgat agcagggttt gctattggat tttacaataa aaatatagta ggtatttttg
      181 ttggagtttt atcaataata atatttatag cactcttagt tattcatagt aaagtaaaag
      241 aagaagaaac atattttaaa agtaagagcg aagtattaaa taagtatatt aagagatttg
      301 gagatggttg gaaggaattt aaaatagatg gtaaagaata cttaaaagat gaaaattcgc
      361 aagcaaaaga tttagactta tttggaagag catcattgta tcaatatatc tgtgtagcta
      421 atacatctta tgggaaaaat tttttagcca aatatttgtg gaatgaaaat ccagatgaga
      481 atgtaatctt agaaagacag agggctataa aagaactttt atcaaaagat gatttttcta
      541 ttcatattca aatactcagt aatataattg gtaaagagca aaaaaataat tcagatagta
      601 gtatagaatc gtttatagag tatggagaaa ataagaatgt gtatatacca aaatggatgc
      661 atatttttac ttggggtcta ccaactgcaa caatcttatc ttttatattt tgtatgttag
      721 gatttttacc tgttttacct gtttttttac tttttataat ccagttaggg tttagtggtt
      781 ttggatatcc aagacttatg caaactcttt ctccattatt ttcatttagt agaagtatac
      841 aagtatatga aaaaatgttt gaggttttag aaaaagagac gtttgagagt gcatatttga
      901 aagaattaca agataaactt tctaagggaa gtggagtttc aagaggtgtt aaacaattaa
      961 attcaatagg aaatgctgta aacctaaggt ataatcagat actatatata attgcctgtg
     1021 gtgttttgat gtggaactat cattgtgcag aagctttgga gcgatggaaa ggtatatatg
     1081 gaaatcaaat tagagattgg tttgaatcta ttggagaatt tgaagctcta ataagtttaa
     1141 cagttatttc tcatgtaaag gaaaatacat gttttccagt aataaaatat gaagatactc
     1201 caagattgaa agttgaagag gtataccacc cattaatagc agaaaaaagt gtcatagcaa
     1261 attcgataca attaaattct cagacttgta ttatcacagg ttcaaatatg tctggaaaga
     1321 caacattttt aagaagtatc ggtgtaaact tagtgctggc atatgcaggt gctccagtat
     1381 gcgctaagag ttttgatgcg acctgtatgg ctatatttac ttctatgaga atacaagatg
     1441 atgtaagtca aggaatatca actttctatg ctgagattct tagaataaaa tcaatgattc
     1501 aatatagttt aaaagaactg ccaatgttgg ttctagtaga tgaaattttt aagggaacta
     1561 actctgctga tagaattatt ggagcaagtg aggcagtaaa gaaattatca aaaccctggg
     1621 ttataagtat ggtgacaaca catgactttg agctatgtga cctttcaggt agtggagatg
     1681 ttgaagttgt aaactatcat ttctcagagt actatgtaga cgataaaatt cattttgact
     1741 ataagataaa agatggaaga tgtaaaacta caaatgcaaa acaacttatg agaatggcag
     1801 gtattcttta attaatagat aggttaatta aagaataagt ctatttagaa atataattta
     1861 atttgtgaaa ttaataagga ttgtcttaaa ttacgtaaaa gtattttaag acaatccttt
     1921 tttaagaata aattttatac tttgttatga ctacttagct tcttcacaag cacattcttg
     1981 tggacactct ccatagacac aactcttatc aaagtaatga gtatgaaggt atttatgggc
     2041 tacatcacta tttggttctc caaagaagtc tttataaata gcttgtaaat caggattctc
     2101 atgagattta cgaatagcct tatttctatc tatttcatat aacgcatttg cacgtctatc
     2161 aactatttca gtattaccat ggtgataagg ttgaccagca cctccaacac atccaccagg
     2221 acatgccata acttctatga tatggtattt acattctcca cttctgacct tatccataat
     2281 ctttctagca tttttaagtg tatttacaat acacacatta actgtagttc catcaacatc
     2341 tatacttgct tccctaattc ctttaagacc tctaacatta gtgaagttta cattatctaa
     2401 agtcttatta gttatcttct catatgaagt acgtagggca gcttctaaaa ctccaccact
     2461 tgctccaaat atagatgcag cacctgtaga ttttcctaga ggattatcaa aatcttggtc
     2521 ttgaagatta ggtaaatcga tagcagcttc ttttatcatt ttagctaact ctctagtagt
     2581 tatagaaagg tcaacatcta gtattccaga ttcacttaat tcttctcttg aagcttcata
     2641 ttttttagcc acacaaggca ttacagatac aacataaagt tcttcaggat tgattcctaa
     2701 tattttaggt gcatagtagt ttttagctat agaaccaaac ataccttgag gagacttaca
     2761 gctagatgct aagtttaact tgtctggata attgtgttct aagaagttta cccatgcagg
     2821 acaacaactt gttaatatag gaagattttc acctttttgg attctttcta taaattcagt
     2881 agcttcttcc attatagtaa aatctgcacc aaagtttgta tcaaatacat gttcaaatcc
     2941 aagtgctttt aaagcagcta ccatttttcc tgtagagatt gaccctggtt ctaatccaaa
     3001 ctcttcacca atagcaactc ttacagcagg agcaacttgc actactatag tcttttcttt
     3061 tttatttaaa acatcccaaa gtttaggtac attatctact tctgttaatg ctccagttgg
     3121 acagacagat atacattgac cacagaatgt acactctgtt tcaaccatat cagagttaaa
     3181 gaatgtacta actaaagtat taaatccacg attaactcca gataatgctc ctactgtttg
     3241 tatgtcatta catacagtct cacatcttct acataatata cacttagaat ggtctttaac
     3301 taaagattta gtagaagtat ctttacctgc aaaagatttg gaaccttgat atcttatttt
     3361 tcttactcct aaatcagcag ctatagtctg taattcacaa tctccatttt tttcacagac
     3421 aaaacaatct tgtggatggt ctgataacaa taattcaaca atagttctac gagcatttaa
     3481 tgctttagca ctattagtct gaactttcat accttccttt ataactgttc cacaagctgg
     3541 aactaagcct ctctctgtct caaccataca aatacgacaa gaagcacaag tgtcaagctt
     3601 atctatttcg ttcatatgta aatgacaaag gttgtgtatt tttatattta ttaatttcgc
     3661 agcatctaag atagaagttc cactaggtgc tgaaacgtgt ttgccattaa tagttaaatt
     3721 aactaaactc attgttattc ctcctccaaa taagttattt cttgattata gcgttaaatg
     3781 tacaattatc tatacaagca ccacatttta gacacttaga agtatctatt gtatgttgtt
     3841 ccttaacaga acctgttata caaccagcag gacacactct agaacatttt gtacaaccct
     3901 tacagtcatc tgttataaag taagaaagta agttctgaca tttaccagca ggacatttct
     3961 tatctacaac atgagctata tactcatctt tgaaatattg aagagtactt aaaacagggt
     4021 ttggagcagc tttaccaaga ccacataatg aagcagtttg tatagtttca gcaaggtttt
     4081 ctaggtcatc taaatcttgt agagtaccat taccttctgt aattttagtt aatatttcta
     4141 ataatcttgt agtacctata cgacaaggtg tacatttacc acaagattct tcaacagtaa
     4201 actcaaggaa gaacttggct atatctacca tacagtcaga ttcatcaaga acaagcatac
     4261 caccagagcc catcattgaa ccgatagaac ttaatgaacc aaagtctata ggagtgtcta
     4321 aatgttcagt tggtatacat ccaccagatg gtccacctgt ttgaacagcc ttaaatttct
     4381 taccttcagg gattccccca ccaatttcaa atacaatatc tcttaaagta gtacccattg
     4441 gaacttcaac aagtcctaca ttttctactt ttccaccaag agcaaagact ttagtaccac
     4501 tagaatcttc agtacctaag ttggcaaacc aatctccacc cttaagtata attgcaggta
     4561 tgttagcaaa agtctcaacg ttatttaagc aagtaggaga tttccataga ccactcttag
     4621 aagaactata agttttcatt ctaggttcac cacgtctacc ttcaattgaa tgcattagcg
     4681 ctgtaccttc accacaaacg aaagcacctg caccatattt taattctaat ttaaagttaa
     4741 atccagtgcc taagatattt tcgccaagta aaccgtattt ttcagcttga gctatggcta
     4801 cttttaatct ttctatagat tttggatatt ctgctcttat gtatatgtat cctgtatcag
     4861 aacctatagc gtatccacat attgccatag cttctagtac actatgaggg tcaccttcaa
     4921 gtacagatcg atccataaat gcacctgggt caccttcatc agcattacat acaacaaact
     4981 ttttatctgt tggaccagct ggattttttg aagcagcttc ccatttagag ccagtaggga
     5041 aacctgcacc acctctacca cgaaggccag aagtttttac ctctgcaata acttcttgag
     5101 gagtaagaga agttaaacat ttgcctagag ctaaataacc atcattggca atatattctt
     5161 ctatactatc tgggtctata agaccacaat tttttaaagc tatacgtaac tgttttttgt
     5221 agaaagacat ttcttcttgt tcttcaactt ttttacttaa agattcttcc tcatataata
     5281 attcctcgac aactgtattt ctaattaagt ggctttgaac tatcttttca gcatcagatg
     5341 gctcaacttt tacgtagaaa acgttatcag gataaacttt tacaatagga ccttgagcac
     5401 agaaaccaaa acaacctgtc aaacgaacac taactttatc ttggattcct gatttcttta
     5461 tttcattttc taattcacta actatttcta gactgtttga agaggtacat ccagtatcac
     5521 aacaaacaag taattctctt cttaatacat tttccttaga ataatttttt cttaagctaa
     5581 gattaggttt cagctcatca gctaaaactt ttaattcatc aaatgaattt actttacgca
     5641 ttatttattc acccctctta acaattcaat tctaattctc tataagtatc taatatttcg
     5701 cttacttggt caggtttcac tttaccataa actttaccat ttacagtaac aactggagct
     5761 aaaccacatg cccccaaaca tcttaaaccc tctaaagaaa atttaaaatc actagtagtt
     5821 tctccaactt ttatgccaag ctgtttttca aaagcactta agattttgtc agcacctttt
     5881 acaaaacata cagttcctag acagacactg attttatact tacctacagg ttctgtagta
     5941 aaataagaat agaatgtaac aaccccatat acctttgcag gagctactcc aagttttcta
     6001 gcaacatgaa gttgaacttc ttttggaaga tatccaaata taccttgggc ttcatgtaga
     6061 acttgtatta acgcaccttc ctttgttgta agagagtcaa taaatatatc tagttcgtca
     6121 aataattgct tgttgtgtga aataaaatcg cacataacaa atcccccttt tctttttaaa
     6181 gttattattt ttcataaaat aattaaccat gagttgaccc caacattatg tgggtgaaca
     6241 aaaccagaac aataaagaaa gaataatata caacaatttc tttatttata aaaacaatgc
     6301 aaaaaagtta cctattaaaa ataaaaacta tactagtcta ttataatata aaataattca
     6361 gaatagttaa aaaaaataaa aaaaatgagc taaattcaat aaaaaaaaaa taatacatat
     6421 aaaaaacttt aaaaaataaa ccaaaatgta aaatttttat aaaataaaag atttataaaa
     6481 ctgctgataa tagtcttgaa attgattcct tgatttttat ataagtagac ctagaattat
     6541 atatatctaa tgttatttcc ttagaattta aaatatcatt ttcaaatata attctttgtt
     6601 ccaatgcttt ttttgaagag tacataaatg cattaacttc gaaattaagc tcaaaacttc
     6661 tgatatccat attagctgtt cctatagagc atatagagtc atctataact attgtcttag
     6721 catgtaaaaa tgcattttga ccataagtgt agattttagc tccaaacttt aaaagttctc
     6781 cagcatatga atatgatgcc cagtatacaa agggatggtc aggtttagaa ggaatcataa
     6841 ctctcacatc aacaccagat aaacaagcta tttttaaagt atctataaaa gttgagtcaa
     6901 gtattaaata aggactctgt atgtatatat actttctagc tttctgaatc attttaaggt
     6961 aaccatattt aatctcatca agttctgata tatctggacc acttgatact atctgaatcc
     7021 caatattttc agaaggatga ttagaatttg tatcttcttc tataaagtac ttttctaaat
     7081 ctaaatcttc tttagttgta tatctccaat caaggatgaa tctcatattt aggtcaatta
     7141 cgcaatcacc agtaagctta gtatgtgtat ctctccatag accaaatttt ggatttctac
     7201 ctaaatattc atcaccaaca ttatatccac ctacaaatcc taccttacca tcaatgacaa
     7261 caatttttcg gtgatttcta tagtttaagt taaagtttac aattttcaag aatgatggga
     7321 agaagcttcc aactttaaca ccactttctt taagtctgga aagtgtacta ttttttagtg
     7381 ttctaccacc aactgcatca aataaaagtc ttacttcaac accttcttta gctttgtcta
     7441 taagaatgtc tattatttca gttccgattt tgtcatcttt gaaaatgtaa aattgtatgt
     7501 ttatatattt tttagctttt ttaagttctt caagaagact gttaaaaaat tgactagatt
     7561 cagcataaat ccacacatcg ttattattgg tataatgagc attatttgaa ttagccaatg
     7621 cataaatcat atccttatgt tgatgtatat ctgaatcaat ttcagatgta gattggagtt
     7681 taacctgagt atctagtata ttacttttga ttatcttatc atccttttct ttcaatctaa
     7741 acatattgtt ttttgaaata tttctgccaa aagctatata aagtatgaat ccaagagcag
     7801 gaatcaatac taatataaga agccatgtta aagttgtatt tatgtttctt ttttctctaa
     7861 agataaggtt tagtataacc atgaaattaa ttatatatat tgtagtaact gctatttcat
     7921 atattgacat ttcgaaaaat gatatatcaa acataaaatt ccctccttca aatatttatt
     7981 aaaataagta attatatata ataacacaaa
//
LOCUS       DAEPOG010000165         7869 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEPOG010000165 DAEPOG010000000
VERSION     DAEPOG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.165"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(23..1429)
                     /locus_tag="KR432_003040"
     CDS             complement(23..1429)
                     /locus_tag="KR432_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0641555.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1474..2181)
                     /locus_tag="KR432_003041"
     CDS             complement(1474..2181)
                     /locus_tag="KR432_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641556.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2343..3266
                     /locus_tag="KR432_003042"
     CDS             2343..3266
                     /locus_tag="KR432_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641557.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3266..4027
                     /locus_tag="KR432_003043"
     CDS             3266..4027
                     /locus_tag="KR432_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641558.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4039..4776
                     /locus_tag="KR432_003044"
     CDS             4039..4776
                     /locus_tag="KR432_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641559.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            5296..5598
                     /locus_tag="KR432_003045"
     CDS             5296..5598
                     /locus_tag="KR432_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641560.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            5810..6079
                     /locus_tag="KR432_003046"
     CDS             5810..6079
                     /locus_tag="KR432_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0641561.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(6296..6541)
                     /locus_tag="KR432_003047"
     CDS             complement(6296..6541)
                     /locus_tag="KR432_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBH0641562.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            7078..7638
                     /locus_tag="KR432_003048"
     CDS             7078..7638
                     /locus_tag="KR432_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBH0641563.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
BASE COUNT         2762 a         1073 c         1092 g         2942 t
ORIGIN      
        1 tctgttatta ttttataaaa tatcacttaa ttagaggtaa ttttagtatt gtttttagtc
       61 cctgactatc aatactcata atatctaatt tacctctatg tgcattaata attccagcta
      121 ctatcaaaat acctagacca tgattttgac ctttggcata tgcctcactt agtccatttt
      181 ggctctttaa taaactatta attctttctt gactcatacc ttttccatca tcttcaacaa
      241 ttatatagca aaaatctaaa tcagacttag aggtaaccct tatattacat ccttgttggt
      301 tatgagttat actattttgt attaaattac taatagccct ttttaataat tttttatctg
      361 catctataat aatatcttca tttagtatat caagttcaac ataaaagtca ttatccaaat
      421 tattatttat aaactcagaa ataagctctc tcaaaaacgc agaaactcta atcttatcac
      481 aattcaatgc ttgcatatta tattcaagtt tagatactaa atttaggtca tttactaatt
      541 ctctgatttt aaccccttga ttgcatataa tagacacttg ctcttgttct ctatttgaaa
      601 ggctactgct ttcttcaagt tctccagcat atccaattat cattgaaagt ggagttctta
      661 tatcatgaga tattcctgca atccagttag acctagcttc ctctttcttc ctaagttttt
      721 catctttcat ttgcaactct ttagatacag tattaataga ttttgaaata tcacttagta
      781 ttcctttttc ttgtaaaagt actggttctc cttttgacat aagctttatg ccagtaataa
      841 ttggttttat agatttcacc atttttaaac ctatgaatag agctattaaa aaagtaacta
      901 ttgcattaac cagaaacata cctattatta acataggaaa ttgttctatt tccttaatcg
      961 tataagtaag gttaaattta gtgtatttgt cctttggata acctgttact aatattccat
     1021 ttggatgttc ccatacatac acaggatagt cctttaaaaa gaaccttgaa aactttgcta
     1081 catctgtcaa agtataatga cttggaattt cttttggtaa atcaaatccc catattctat
     1141 ctccattgtt atcaattaac attgcccaaa ctttattttt atgtagtact tccttactag
     1201 aatcatctaa aataaacttt ccatctttta aaatcaaatt atcttcaatc ataatcaagt
     1261 ttgattttat ttctctgcta tcacttgtat ctaaattatc agagtattta gatacaaata
     1321 gcatatatga aaacagaaga aaacctgcta tatttaatac aataaaaagt aatgaaatca
     1381 agatacttga acctataaat cgcctcaata tgtactttac actatccaaa attaaatcac
     1441 tcctcttgat ttcttttatt ctaatatcta aaattaatta atcatcaact tatatccaag
     1501 acctcttact gtaataagat gtttaggtgt agatggagtt ttttctatct tctctctaac
     1561 ccttctaaca tgaaccataa gggtattttc atatccatac atatcatctc cccaaatggc
     1621 ttgacatagt gaatcattag taacaattct ccctttattt tcatacaact ttaaaagcaa
     1681 cgaatgctct ttagctgtta aagaaatctc ttgcccatct ttctcaacac atgcactatt
     1741 taaattcaca acaacagaat ctcccagttt aaatactggc aactcctctt cttctatagg
     1801 cacatataca cgatttaata tacccacaag ccttaaagta agttcctttg gtaaaaatgg
     1861 ctttactata taatcgtcag cccctaatcc caaaccaatc aatctatctt catcttcacc
     1921 tcttgcagag agaaaaataa ctggtatctt agatgtctct ctaatttttc tcataataga
     1981 gaaaccatct ccatctggca atacaacatc taaaattgca caatctggct ttacatttct
     2041 aaatacccta acagcttctt cataacttga tgctaaaaat actctactaa atccctcttt
     2101 tcttaggatt tcatctatca tttttaataa ttccttgtgg tcatcaacca caagtatttt
     2161 tttatctttt attgactcca taatcatcac cctcctttag tataccatat acccattttc
     2221 acaactttgc ctaaatagac ttttaaggtt attgtaaggt aaacttcagc taactgaaat
     2281 gtaaccaagt tattatactg atataagtta aaagaatcaa taagcacaga ttaaaaaagg
     2341 ggatgaaaca tatggaaatg ataattagaa cacaaagact ttcaaaggaa tacaataaaa
     2401 tatttagagt aaaggatata gacctaagag ttccacaagg agctgtatat ggttttttgg
     2461 gtcctaatgg agctggaaaa tctaccactc ttaaaatgct tcttggactt gctaaaccca
     2521 cacaaggaaa tattaatatt ttaggtaaag agttaaatga aaaaaataga atatcaattt
     2581 taaaagatat aggttctttg atagaatctc catcatacta tggtcatctt acagggcttg
     2641 aaaacatgac cataatgcaa agactcttag atttacctaa aaaaaatgta aatgaagcat
     2701 taaaaattgt aaggttagaa aatcaaaaaa ataaaaaagt taatcaatat tctttaggta
     2761 tgaagcaacg tcttggcatt gctatggcaa taatgaagtt tcccaaatta ttaatacttg
     2821 atgaacctac aaatggactc gacccagcag ggattgagga aattagagaa ttaataaaat
     2881 ctctaccaga aaaatacggc atgactgttc taatctctag ccatctttta agtgaaattg
     2941 accaaattgc cacatcaata ggaataataa atcatggcga actggttttt caagatagca
     3001 taaaagaact tcataataga ggtcaaagtc agattgctat caaagctaag aatgttgtat
     3061 ctgcacaaaa gctattaaat actaaaggat atatgtcact tatacaaaat gggtacttga
     3121 ctattgaaaa tttaaaagac tcagatgtgg ctaaaataaa tggtatgctt gtccattcaa
     3181 atatagatgt atttagaata gaagaacata aaaaaagctt ggaagatata ttcctagaac
     3241 ttactggtaa ggcggtgact ttataatgaa ataccttggt ctagaatttt ataaacttaa
     3301 aagaaagaag attattctga tgaccttttt atttgtagct gttgaaataa tgtggtgtat
     3361 cgtaaattca aatagggcta ttacacgtaa tccagatatg ttaggtggat ttgaatattc
     3421 ctatatgctt atgagttttg caagtttaaa tggtctattc tttcctatct taatatcaat
     3481 aattacatca cgcataagcg atatagaaca taaaggagat acatggaagc ttttaaaatc
     3541 ttctgtgaca tctttaaata gtatttattt atccaaattt ttatgttctg ctattttggt
     3601 atctatacct atattgatgc aagtgttatc tattgtgttg tttggacatt ttagaggggt
     3661 tattgagtca atttctgtat ctttactttt aaaatacaca ttaggaacaa ttcttgtaag
     3721 tattgctatc attgcactac acctatggat tgcaactgta attccaaatc aaatgattgc
     3781 aattacattt ggcatgattg gtagttttat aggtttgaca tcaggtttgt tttatcaagg
     3841 tattcgtaga ttgtttatat ggtcttatta cctagagtta tcacctttaa gttatgctta
     3901 tgataatgtt ttaggttcat ctgtctatag aattgatatg aatttttcta ttacgatttt
     3961 agttttctta attggaatgc taatttatta tattggaaga aatcatttat taaaaaaaga
     4021 agtataggag ggatttaaat gttaaggaag tcagttttgg gagaaattct aaagttaaaa
     4081 aattctttta tatggtatgt attattagct ttgccactta taagtgtgtt gattggttct
     4141 ggtaattttt atctaaatca aggcatactc aaaaaagaat ggtacagctt atggactcaa
     4201 gttagtcttt tttatgggga atttttcttt cctatcttaa tcgcaatctt ttgtgcatat
     4261 atttgtaggc ttgaacatat gaatcataat tggaataatg taatgacact gccagttaat
     4321 ttaaaaaata tctttttatc taaacttaca gtagtttcaa tacttacagc tattacacaa
     4381 gttttctttg ttatctttta tatagttgca ggaaatatgt ttggtttttc tggtagtgta
     4441 cctaaagaat tgactggttg gattgctaaa ggttggatta tttctatttc aatagcttct
     4501 atacaattat acatgtctat aaagattaga agttttgcaa ctcctattgg tattagtctt
     4561 tgcctttctc tatttggtat gggattaata gtagcaaaac ctgctgttgg tatatttttc
     4621 ccttattcta tgttaggtag tgctatgggg gttataaagc aatctgcttt aaatgttaat
     4681 gacactatag ttacaatatg tgttgcattt attactacta tagtattttc tgaaattgcc
     4741 aatagaaatt ttagaaagaa tgatgttatt tcttaatggt aaatgctaga tattttttgg
     4801 tttgaaattt atattctttt tggtttgaaa tttatatccc ttttatttaa aaatatatat
     4861 cttttatttg aattttatat gttttatcta ctcactatat aatagatata ctcctaaaat
     4921 aaaaaaatat ataataatgt tattatttaa aattattatg tgttatatta aaacttaatg
     4981 caaaaacaat ttttatatta tataatgaaa cttaattaaa tatagctaaa aaatcattca
     5041 atacattaaa taaatattta actttaataa tctatattta attatggcta taacatagaa
     5101 ttatcaaata tattgaatga atatttgact cttggaatgt gtaattaaat taagtttggt
     5161 tattcgatgg aaggcaaatt tgatataaaa actccattta aagtgccaga taaaaacttt
     5221 atggttatat aaaagttaaa agtaatttat agtgttatag aatattcaaa gggatttaaa
     5281 gtaatctatg gtgttatgaa atattcaaag ggatttaaac taatctatag tcttgtagaa
     5341 tattcaaaga aatttaaagt aatctatagt gttatgaaat attcaaaggg atttaaacta
     5401 atctatagtc ttgtagaata ttcaaagaga tttaaactaa tttatgatgt tatgaaatat
     5461 tcaaaggaat ttaaactaat ctatagtctt gtagaatatt caaagagatt taaagtaatc
     5521 tatagtgtta tgaaatattc aaagagattt aaagcaatct ataatgctgt gaaatatatt
     5581 caaggggatt taaagtaatc tatagtgtta tggaatattc aaagggattt gaagtaattt
     5641 attgtgtttt gaaatattca aaaatatttt tggaatagtt tttataaatt taccagtatt
     5701 aataatttta tgtttaacat ttagatgact taaaatcaac tatttgcact tttgtagtca
     5761 tctaaaatca ttttataata tttttaaata atggaaagga tgtttgaaca tgtttgaatc
     5821 aagatgtggt gtctgttgtg acagttgtac aaaaaaagaa caagtaaatt gcactggttg
     5881 cccaactatg gaaaaaccat tttggggtgg agaatgtaag gttaaaactt gctgtgaaag
     5941 taaggaactt aatcattgtg gtgaatgtga tactttccct tgtgatatgc ttttaaataa
     6001 gggaaaagac caaggctttg accctatggt aaatattggg caatgtagaa aatggcttga
     6061 agaaacagtt tctaattaat tgttttagaa tgctttgtta agtaatttat ttaatccttt
     6121 gttaggtagt atgtttagtc atttattgat tggtatattt atacatttat tgatgggtat
     6181 atttagccat acaaatagat ttacataaaa tttacgacta aaaaagagtt gagtattagt
     6241 ttttattatt taaacggata ttcaactctt ttttatatct aacttttttg attttttaat
     6301 ctttccaata ccaccacttt aatttttctg gttctggatt ttcagttgat gcaaaatata
     6361 ctgcacatct gtatacatat aactgacatc tgtctatgtg ctgtccacgc attaaacatt
     6421 ccttttcata catttgtgct gggtttgcat ctttaagtga tttaatagtt gtatatccta
     6481 acatgattaa agatttttct gttgctttcc ctacccctgg aattttttgt agttctccca
     6541 tacttcacct tctttctgat atattatttt tgtattatac ttagtaccag atattttttt
     6601 attatagtta atatttaatt ttttattata tcactttatt tatgctcttt catctatcta
     6661 tattttacca cctctaaagt actgaatcat ttaattacat cataatatag ttttatacaa
     6721 ataaaatact ttatgtttca tttaatatat aaaattcacc ttcaagaaaa ttatattata
     6781 atctgacatt tttacctcat ttttcaaaat atattgaatc ttcttgattt attttgtaaa
     6841 attatgctta ggggaaatat attttaggaa aatatgaata tataattttt agtcaactag
     6901 ttattttaag tttttaaatt ttaaaataaa atatatctaa taaaagggag attgtattat
     6961 gttttctaaa aaaaatgagg gtaacgaatt tagtaatgaa agaaaaggaa gctctaagaa
     7021 aataattaaa ttctttaaga gcacaaagga tattgctcta ctggcattta ttttggtgtg
     7081 ttttttggca atatatcctc accagcttgt tctgaagacc atgaggaggt catttctaat
     7141 caaacatcag ttatagattc tcaaaaaaca gaaatagaaa ctttaaatag caaattgtct
     7201 gatgctgaac catggttcaa aatgaaagac gacgaaaaga aagctattga agctgaaaat
     7261 caacgtaaag ctgaagaagc taaaaaggct gaagaacaac gtaaaaaaga agaagaagag
     7321 aagaaaggat atgatactgg tattacttat gaccaattag ctagaacacc tgatgattat
     7381 aagtacaaaa aggtaaaatt tgaaggtaag gttattcaag ttattgaaga tggtgatgag
     7441 gtgcaaataa gattagctgt gtctggaaat tatgataagg tcgtactatg tagttataaa
     7501 aaatcaataa ctccttcaag agtgttagag gatgattaca taactataag aggtataagt
     7561 gctggaacta taacttatga atcaactatg ggtggaaaaa taaccatacc agggatagct
     7621 gtagagaaaa ttaattaata ttgttttgta ttatagttaa tattttatat tatagtcaat
     7681 atgtttaaag atgtttttat aattgcaaat aaacagttac aaggctctaa attagttttt
     7741 gcttttagca tattatctat tttctatcaa ctattaatta tttagtatta atatttccat
     7801 atatgaattt tattataaaa tagtcaagaa taatagatta ttaaatgata gaaaaatttt
     7861 aactaaaag
//
LOCUS       DAEPOG010000166         7779 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEPOG010000166 DAEPOG010000000
VERSION     DAEPOG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.166"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(11..724)
                     /locus_tag="KR432_003049"
     CDS             complement(11..724)
                     /locus_tag="KR432_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBH0641564.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1016..2680
                     /locus_tag="KR432_003050"
     CDS             1016..2680
                     /locus_tag="KR432_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBH0641565.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2787..3686)
                     /locus_tag="KR432_003051"
     CDS             complement(2787..3686)
                     /locus_tag="KR432_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBH0641566.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3713..4351)
                     /locus_tag="KR432_003052"
     CDS             complement(3713..4351)
                     /locus_tag="KR432_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0641567.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4688..5686)
                     /locus_tag="KR432_003053"
     CDS             complement(4688..5686)
                     /locus_tag="KR432_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBH0641568.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5784..6359)
                     /locus_tag="KR432_003054"
     CDS             complement(5784..6359)
                     /locus_tag="KR432_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641569.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6675..6938
                     /locus_tag="KR432_003055"
     CDS             6675..6938
                     /locus_tag="KR432_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641570.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7367..7744)
                     /locus_tag="KR432_003056"
     CDS             complement(7367..7744)
                     /locus_tag="KR432_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641571.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         2715 a         1203 c          830 g         3031 t
ORIGIN      
        1 ttctatattt ttaaccaaca gatgttttct tcaaattaat cagatgattt agtatatctt
       61 gaacctttcc agagtccttt aaatatccta atgccccatt tatttgtgat tttaaatcta
      121 tttctacttc catatccaaa tcaacatttc ctttttttat agatatattt ttaagtgtta
      181 ttgggtaaag ataacttttt tccaaagtaa tcttatttcc attaacctca aatggaactt
      241 ttttattgta gttacttaat attttttcta gcattttatc acttagttta aattttccaa
      301 ttttcacatt ctcaagactt acaactaagt ttccatcctt taaagtaggt ctcaattcaa
      361 gttcatattg actatttacc aaaccaaata ccttatatgg tcctgctacc tttattttac
      421 catttttcat ttcaacaaaa ttattttcaa gttcattaat tccatgcttc tgcatcaatg
      481 tatatattaa atttttaaac tcttcctctg aaacagatat ttttccaacc attctaagtg
      541 gattttttac tacctccata gaatttatat atgagccttt tgataaatat tcttcatcta
      601 tgattttatt attttgtgaa ctaacatcat atctttcttt tggtgtcaag atatatacaa
      661 caattcctgc aagtactaaa acaataacaa ttaaacttaa caaaatcttt gcaacctttc
      721 ccataatcgt ctcctttaca aatctgtcat cagatttaaa ataaatttag ataactaact
      781 tcacataata ttcttaacaa tatacatact ataatacagt cttttaaaaa ttaattcaac
      841 caatctaatg ttttgtaaat aaactgtaaa aacatgtttt tagtataaaa gttaatattt
      901 tgcataatac tcttaattac acctttattt ttttctaatc ttggattaaa attaatatta
      961 tgcaatacag tgtagtgttc tgtattataa tcaaattaaa taggaggtat agaatatgtc
     1021 aaatgaaacg aactcatcta actttataaa gaatattatt ataaacgact tagaaacagg
     1081 aaaacatgac agtatcataa ctcgttttcc acctgagcca aacggatatt tacatattgg
     1141 tcatgcaaaa agtatatgtc ttaattttgg attagctaag gagtttaatg gaaaagctaa
     1201 tttaagattt gatgatacaa acccattaaa agaggatgtt gaatatgtag agtccataaa
     1261 agaagatgta aagtggttag gatttgattg gaacgagtta aattttgcat ctaactactt
     1321 tgatgaaatg tataagagag ctttaatact aataaaaaag ggtaaagctt atgtatgtga
     1381 tttgacacaa gaggaaatga gagaatatcg tggtactcta actgaaccag gaaaagaaag
     1441 tcctcataga aacagaacta ttgaagaaaa ccttgattta tttgaaagaa tgaaaaatgg
     1501 tgaatttaaa gatggtgaaa aaacattaag agccaaaata gatatgtctt ctccaaatat
     1561 aaaccttaga gacccaatta tatatagaat atctcattca acacaccata atacaggaga
     1621 taaatggtgc atatatccta tgtatgcttt tgctcatcca attgaagatg caattgaagg
     1681 aataactcac tctatatgta ctttagagtt tgaagaccaa agacctcttt atgattggtt
     1741 tgttaaagaa tgtgagatgg aaaatatacc tagacaaata gaatttgcta gacttaatat
     1801 aaataatact gtcatgagta aaaggaaatt aaaacaactt gtagatgagg gtattgttga
     1861 tggttgggat gaccctcgtg ttcctacaat atccggaatc agacgtaagg gatatactgc
     1921 agaagcattg cgcaatttct gtagtgaaat aggggtatca aaagttaact ctacggtaga
     1981 tagtcaaatg cttgattatt tcctaagaga aaacttacaa ccaaaggctc ctcttactat
     2041 gggagtttta agacctttaa aacttattat aactaattac ccagaagata agattgagat
     2101 gcttgaaata gagaataatg ctaaagatga atctcaagga aagagattag ttcctttctc
     2161 tagagaattg tatatagaac aagatgattt tatggaagaa cctgttaaga aatattttag
     2221 atttttccca ggaaatgaag tccgtttaaa aggtgcttat tttgtaaaat gtactgatgt
     2281 aattaaggat gaaaatggaa atgtagttga aatacatggt acttatgacc ctgaaactaa
     2341 gagtggctct ggctttacag gacgtaaggt aaaatctaca atacattggg ttgatgctaa
     2401 atcagctata ccatgtgaat ttagattatt tgaaccatta attcttgatg atatacctga
     2461 aaatgaaggt aaacatttct tagaacaaat aaatcctaat tcattggaaa tattacaagg
     2521 ttttgttgaa cctacacaga taaaagattc taaacctttt gataaattcc aatttgttag
     2581 aaatggattc tttagtatag ataataaata tacaactgac gaaaaatttg tatttaatag
     2641 aatagttcct ctaaaaagtt cttttaaacc aggtaaataa tcttacaatc aaaaaatgaa
     2701 gacttaaaat ataaattact tctactataa ataaacttag ttagaagtaa ttttttattt
     2761 gagtcttcat catttacttt aaatgattaa cttttttcac tacagattcc cataacttct
     2821 attccatctt ttatgcattc tatgttaaga ggaaaatctg gtcctttttc tccgtcaata
     2881 tctgttataa gtgaatcttc acattctata tgcacctttt tagttttaaa gtatagtatt
     2941 tcatttccat tatattgatc taaataatca cctctaagca cacttatcaa aactggtatt
     3001 gattttggta ttggcattcc tttaaaaata attacatcca aatatccatc atctatttca
     3061 gccttatatg ctagatttat atttcctgct gttttgccat taaaaatcaa cataagatac
     3121 atatctccat cataatacat ttcctcagaa gatactttta tcttaaattt cctcatatac
     3181 aatgcttctt ctattccctt tatataataa gaaattcttc caaatgaatt ttttaagtct
     3241 gtgtttatcc tttgtgatac atctgtaaac ataccagcac ttgcaacatt tataaagtac
     3301 ttattattga ctttccctat gtctattttc tttggttttg aatttatgat ttcatttata
     3361 gcttctttta tatcaaatga aatttgaaga gcatttgaaa aatcatttgc agttcctgta
     3421 ggcaaaattc caattggaat ttttatgtct aattctttca ttgcatttac tactaaatct
     3481 acagtaccat ctccaccagc tatcaatatg tgttcataag aactatcaac ttccttaaat
     3541 gcattcttta caggttcttt aatagttaat ctatagggta tcaataaata atttttttct
     3601 tgatataatt ctatgatagt gtctaaattt gataatatct ttttttcacc tgaattagga
     3661 ttgtatataa gttttacttt tttcattatt tctccttact cattttattc ttttagtatc
     3721 caaaatcttc atttaaaaaa atcacatgta ttctttctgg tgttaattgt ctttttataa
     3781 gtgtataact acagcatatt cttcctggac ttgaacagtc tatacatata ccagtagtct
     3841 tacatccagt atttgtactt aatcttttac aattggctgg agcagctatt gtttttgttc
     3901 tttttatagc ttcatctaca tctttaacta ttttatttac tcctgctata actattactt
     3961 tatcaggtcc atatatcata gcagcaactc tattcccatt cccatctaaa ttgtaaagtt
     4021 ctccatcctc tgttatcgca ttagtacttg taaaataagc atctgcagaa aatgactttc
     4081 tatatatttc tttcatttct tctggtttga tatcttttgc atatctatca agaaaattat
     4141 atttttctct tataaaatca atcactcctg tttcaaataa ggtcatagaa cctccacatc
     4201 ctactagaga acctacttta actatttctt caattttttt aagaagctct tctttgttac
     4261 ttactaaata ccctttcata ttattgtttt ccagagattt tattgttttt tctattcttt
     4321 tttcattaag ccaatttaaa ttttgatcca ttttaacttc ccccttattt ttaatatgta
     4381 cttaattatt tataaacttt attgtcttta taagtttatt ttagtacatc taaaaaaaaa
     4441 tacaatataa aacttaaata gttttatata attttttcca aaataactta ctcaatcaaa
     4501 aaaggaaagt agatgtacta ttctactttc cctatattgt atgtagtatt tattgcctaa
     4561 aatctatata ttaattatat gtttcatatt atataatatc acagttaatt aaaatgtaat
     4621 actatcttct ttttaaacat ataaattgta ttattaaatt taatatatcc aacttcaata
     4681 aatcttatta atttgatgaa gaagatgcta tagctgcact tgttgctgcc attacacatg
     4741 caatttgaat agcaacaaca atatttaaaa atatattatt agttatagag cctatattat
     4801 tctcatttcg tatcaaatct gcataaattg aagctacaat agcagaacta atcattactc
     4861 tattactttt tcctaaagac caatttccaa agcctttttc ttctttaagt ttatttgaaa
     4921 cattttttat ctcttctatt atttcatctt ctctataatc taataatgaa attgctccta
     4981 ttattggata tccataataa ttcattttac aatcattttc tttaaatgct tttttaagtt
     5041 caatagtctt tttacattta agcatagttc tatcatcact aaataaaaga atttgggata
     5101 atgcttgtaa atcatttcct ttataaaaac cattttcatt tagaaattca taacattttt
     5161 ctatgtattc taaatcttca tctaaatttt ttgaatgaat tgcaataagt gctatattac
     5221 aatagtcatc actagaagtt agaaatggat gttttttcct catgtaatca tatattattt
     5281 tcatttttga tatacatgta agataatcca ttttctctct attttcataa actattgatg
     5341 ctgtaagtgg taaatatgta tcgccccaaa acttttcact tttaagttct ttgtatatat
     5401 ctaaaatcat cttaaaacta ctttctggat ttgattcaaa cgaaagtagt gttgccatat
     5461 aaaacatatt atatcctcta aaattagaga ataatccagt atttttttta ataagctcta
     5521 ttcgttcttc cactaatcta ggacttattc tcttgttctt aagagtaaat gcaagtgcac
     5581 aactatgttg tatcaatccc atttgccaag aacctttaac ttcatttaat gctttgtaat
     5641 tttctattaa taagtttact tttccattta taaacgattg ttccatattg ccctcctagt
     5701 aatatatctc attgtactaa tagttttata tttattatat atatattaaa tactacaatc
     5761 aatacatatt acttcaaaga acatcattct tcaacaataa tgtagccttt tttgtaatgt
     5821 cttcccatat tattgactct atcatagtac tctgattcac tttcattact ccatttgggc
     5881 acaaatacat ctattgcatt agaattacta aaatacttac tagaccccct atccataaca
     5941 gtttttaatt ttccatctac aataatctta gttccaaaac tcaagtgatt gtttgctaca
     6001 aaaccatctt ctaaatcatc tcctgtagca gttatcgctc catatccatt ttcttgatta
     6061 agacttgtgt aataactaac ttcaaaagtt ataggtacta acttctttat tttaatacct
     6121 aactgatttt gtagtttctc cttttgtact ttcaacttat actcatcact atttttataa
     6181 tcttccatac cttgattata cttggaatta aagcttttac ctgactttac tatagtatct
     6241 atatttacta tagattcttt acaatgatct gcatctattt ttgtttctcc atttaccaat
     6301 aatggagtta aacatacact acatacaaca catgtaatca atgcttcttt aagcttcata
     6361 aattcccccc ttattatttt gatatattag catatttaat attatatgtc tatatttaga
     6421 tttaaatacc acacccattt gctttaagaa gttgtcttat atttaattca ccttaaacac
     6481 ttataaatcc tctatcttag aatcatatag aataaagctt ttatcgttaa atatttttta
     6541 ttaatctttt aaaatctaaa gctactttat ttaattttct cttttttaga atttaagaat
     6601 taaaattcta taattttaaa acatattgaa tttatttttt tgagatgata taattttctt
     6661 aagaggtggt tgatatgata aaaaatacaa aacaaattac agtagaagtt aatgaaattt
     6721 tattacaaga aatatctaag ataactaatg acgaaaataa tattttgaca gatttgataa
     6781 atgaatcaat tgtaaattat attcttgaaa acaaaaaaca taacattgaa aataaagatt
     6841 tagaagaagt gattaaattt tacaaaaaag aaaatactga aaaacctaaa agtgcagcaa
     6901 gagctatagc tgaagcaaaa actcataccc tgtattaatc aaataaaaaa gttcctaaaa
     6961 actaggaact tttttatatt aataattatt aatatatctc aatatttatc tcaaatttat
     7021 caatataagc atgtttaata caatctattg tatacttatc ttaccctaga aataatcaaa
     7081 acaaaaatta tgccctttat atctaacaat taatttacat gattaatata caaaaattct
     7141 gacttttaaa ttcgtataca atatcaaact aaaataatac taatgacaaa cccaatataa
     7201 attaaattca aacaggtttg attttacatc aaacttattg tattaatatt aaataacaag
     7261 acaaaatatt tataaaaatt acaaatttta cataaaactt tatattttat tcatttaact
     7321 aattattgta aaaactattt attttatgat aattaactac accaaattat tcatcttttt
     7381 taatatcttt aacccatttt tcaacagagt tcaattcttc ttcatttacc ccctttttga
     7441 acaaggattt cataaaactc tcaatagaac ttccatgcat attatctaaa aaagtttttg
     7501 tttcaaaatg taaatactct tcttctccaa taagaactgt ataatgtgta tgtctatcaa
     7561 tctttcttgc acttaagaat tgcttattta ctaatcttga taaaagtgtt agtgttgtag
     7621 tttgcttcca attatatagt ttttccatag cttcaatgac atcttttgat gctactgtaa
     7681 aatctgaact ccaaataaat ttcatgactt ttaattctgc tggtggtatc ttagtaatag
     7741 tcatatgatt ttacactcct ctcaaacttc tacataatt
//
LOCUS       DAEPOG010000167         7755 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEPOG010000167 DAEPOG010000000
VERSION     DAEPOG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.167"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            44..355
                     /locus_tag="KR432_003057"
     CDS             44..355
                     /locus_tag="KR432_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0641572.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      448..540
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            640..1434
                     /gene="thiD"
                     /locus_tag="KR432_003058"
     CDS             640..1434
                     /gene="thiD"
                     /locus_tag="KR432_003058"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH0641573.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1458..2246
                     /gene="thiM"
                     /locus_tag="KR432_003059"
     CDS             1458..2246
                     /gene="thiM"
                     /locus_tag="KR432_003059"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH0641574.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2269..2901
                     /gene="thiE"
                     /locus_tag="KR432_003060"
     CDS             2269..2901
                     /gene="thiE"
                     /locus_tag="KR432_003060"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0641575.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3040..3342
                     /locus_tag="KR432_003061"
     CDS             3040..3342
                     /locus_tag="KR432_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641576.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3408..4157)
                     /locus_tag="KR432_003062"
     CDS             complement(3408..4157)
                     /locus_tag="KR432_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641577.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4151..5113)
                     /locus_tag="KR432_003063"
     CDS             complement(4151..5113)
                     /locus_tag="KR432_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641578.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5117..5575)
                     /locus_tag="KR432_003064"
     CDS             complement(5117..5575)
                     /locus_tag="KR432_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0641579.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5931..6287
                     /locus_tag="KR432_003065"
     CDS             5931..6287
                     /locus_tag="KR432_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0641580.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6359..7075
                     /locus_tag="KR432_003066"
     CDS             6359..7075
                     /locus_tag="KR432_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641581.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7068..>7755
                     /locus_tag="KR432_003067"
     CDS             7068..>7755
                     /locus_tag="KR432_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641582.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINII"
BASE COUNT         3011 a          896 c         1267 g         2581 t
ORIGIN      
        1 ataatataat atttatatat ttaatgttag gatgtgattt aaggtgtatt ttacatatat
       61 tataagatgt aaagatgatt ctctatacac tggatatacc agtaatatag ttagaagaat
      121 gaatgaacat aagttaggaa ttaattcaaa atacacaaga gcaaagggat ttgaaaaact
      181 agaagtttat tttgtgacca acacaaaaag caatgctatg aagttagaat actatataaa
      241 aaaattaact agaaataaaa aattgtctat aataaaaaac ccaagtatac ttataaattt
      301 aattgataat aaagaagatt atattattgg taaggaaata gaacaattaa cataaatatt
      361 tatagtaaca ttataatggg tgcatatagt gtaaaaactt gacaataaat actattgtaa
      421 tataatgaag ttataaatat agtaaattaa aatagctagg ggagccaaga gctgagaggt
      481 ataccgaccc ttacacctga tctggataat accagcgtag gaaagcttag taaaaattca
      541 agtatgtatt atttgtgata tatgctattt ctaagctata cctattgtag gtatagcttt
      601 tttaattaag ttatttaaat taaactttag gaggcaacaa tgagtaatta taaaatacca
      661 acacttacaa tagctggctc tgattcatct ggaggagctg ggattcaggc tgatttgaaa
      721 acgtttagtg caattggtac ttatggtatg agtgtaataa cagctataac agcacagaat
      781 actaaaggag tttttgctgt agaggattta aataagaaaa taataaaaaa acaaatagag
      841 gcagtctttg aagatatacc tccaagagct gtaaaaatag gaatggtttc aagtcctgag
      901 attatacttg aaatagttga gaatctaaag aaatacaatc caaaatattt ggttgtggac
      961 ccagtaatga tatctaaaag tggatattat ctattaaagc cagaggcaaa agaaaactta
     1021 attaagtatt tgataccact tgcatacata attacaccaa atataccaga ggcagaagaa
     1081 ataacaggaa taaaaataca taatgtagat gatatgaaga gagtaggaga agaaatacta
     1141 caacttggac ctaaatttgt acttatgaaa ggaggtcatt tagatggaga agcagtggat
     1201 attttagttg gtaaaaatat atttaaagtt tataaaagtg aaagaattga taaaaaaaat
     1261 acacatggaa cagggtgcac attatcttct gcaataacat cttatctagc attaggatat
     1321 gaaataacag aagcagtaaa tctaagtaaa atctatataa cagaagcaat aaaaagaagt
     1381 tttgatatag gtcatggagt aggaccagta catcattttt ataaatttga gtaataaaaa
     1441 aatattggag gtagaatatg tataatttaa ttaaagatgt aaaaaaatta aatccattgg
     1501 taattcatta tactaataat gtaactataa atgattgtgc taatgtgact ttagctgtag
     1561 gagcaagtcc tcttatgagt ttttcatatg aggaagtaga agaaatggta agtgtagcaa
     1621 attctgtagt aataaatata ggaactatga actcaaatat gttggattta tttctattag
     1681 ctggaaaagc agcaaataag tacaataaac ctgttgtttt ggacccagtt ggagtatttg
     1741 caagcaaagc gagagcagaa cttacaagta gacttttaaa tgaagtcaaa tttagtgttg
     1801 ttaaagggaa tgtatcagaa ataaagttta taggtggctt taatgtaagg ggtaaaggtg
     1861 tagattcatt tgatgaagaa gaagattcta ctgagataat aagaaagatt gctgaaaagc
     1921 ttgaatgtgt agttgtagca actggaaaaa tagatataat aactaatggt aaaggtatat
     1981 ataaaataaa caatggaact gataaattaa aaggaattac aggaactgga tgtatgactg
     2041 ctagtttgat tgcaagtttt atggctgtta ctgagaatat attagaagca gcaacaatgg
     2101 gtgttcttac aatgtcttta agtggggaat tagctaattt aaataatcca ccaataggta
     2161 catttaaaga aaatcttatg aatgctattt atcaaatgga tatagatact ttaagtaaaa
     2221 attctaatat tgaattttta aattaataaa gagtaaggga gacttaaaat gattgataaa
     2281 gaatctttaa aaaaatgctt aaagttgtat ttggtaactg actctgaaat gcttaaaggt
     2341 agagactttt ataaatgtct tgaagatgca atatcttctg gaataacaac tgtacaactt
     2401 agagaaaaaa atgcttctgg aagagagttt ttaagaaaag ctatgaaact tagagaaata
     2461 actgaaagat atggtgttaa gtttataata aatgatagag tggatatagc acttatttgt
     2521 gatgcagatg gagtacatgt aggacagagt gacattgatg tgagagaggt tagaaagctt
     2581 ataggaaaca ataaaattct aggagtatca gcaagaactt tagaagaagc tacttgtgca
     2641 aaaaatgatg gcgcagacta tttaggtgta gggtctatat tcacaacatc tactaaatta
     2701 gatgcaaaat cagcttcgtt tgaaactgtt aaagaaatta aagaaaaagt agacatgcca
     2761 tttgtgctaa ttggaggtat aaatttagac aatatagata aacttaaatg tttagaaagt
     2821 gacgggtatg ctataatatc agctatatta aaagctgaag atatctctaa agaagtggaa
     2881 aagtggacat taaaaatata aaattggctc tggtagtgaa aaaaatggaa aaatttgata
     2941 taatctaaga gtaaaattta agcaattata aatgataaac ttttctacat atacttttta
     3001 atcaagatat atgtagattt ggttaaaaag gagctataaa tgattaagat attgttagtg
     3061 tgtgttggag gcatgtcttc taccctattg gtaaataaaa tggagaagga tgcaaaaaaa
     3121 agaaatatag actgtaaaat atgggcagtt ggagaaggag atataaaatc ggagcttgat
     3181 aactttgata ttttacttct tggaccacaa cttagattta tgcttgatga cgtaaagtct
     3241 atagttggag atagagttcc agtatctata atagatatgg taaactatgg tacttgtaat
     3301 ggtcatgctg tgttaaattc ggttttagaa atattaaaat aaataataaa agtggattct
     3361 atataataga gtccattttt attatttgtt attaataggc ttaaaactta gcttaccatt
     3421 tcttttttta atctccacat agcaagtgaa aaacttgaac agcacaatat taaaaatagt
     3481 aggataacag gcagtatatc tccaataaga atagtctcac caaatattaa gctacgtaaa
     3541 gcgttaataa catgggtaaa tggattgagc atcactgcta cttttaatcc tccagataaa
     3601 ctttctatag ggaaaagagc tgaacttaaa aagaaaatag gaagtacaat tgcagtcata
     3661 attgtttcat aaactatttc atttggcaat aataaactta tagaataagc tagacttgat
     3721 aaaaagaagg ctgtcataaa tatcagtact atcataagta ggattccacc aatgccagat
     3781 tctatcctta ctgagaagaa tatacttact atacacaaaa tagttacttc tatgaaggaa
     3841 actaagatag cttctaataa ttgaccaagg acgattgaat acctactgat aggtgcaatt
     3901 aagactctat agaaacttcc actgttcttc attataaagt ttataatacc accactacta
     3961 caagaagaaa acacaaccag tacaatgata cctggtagta taaaagcagt ataattattg
     4021 atatttatat tattcatagt ttggtttgca atagaactat aaagcacaag ccaaagaaaa
     4081 ggttgtaata tagtaactac aaatgagatt ggatttttaa aacgccactt catactacgc
     4141 cataagatat ttaacattcc cattcctcct ttaagctttt tttagaactt gtaagtgcta
     4201 aaaatacatc ttcgagactt ggctctacaa tttctattgc atcaaattct atatcatgtt
     4261 ctaaaagcca tttatttatt aaagtaaaag cagttctact atcgtttaca cttataaaaa
     4321 ttgaattttc tcgtacgctc ataaacttta ctaaaccagt acttttaatt gaatctttat
     4381 atttttttat atcttcagta ttatggaaag ttatacgtag tatattttgt ctaatgtaac
     4441 tacgtaaact tgatggtgtt ccttgtgcta aatcctttcc attcttcata atacaaatat
     4501 tatcactcaa ttgttcagct tcttcaagat aatgagtagt tagaaatata gttgtgccgt
     4561 actcatctct aatttttaat aacatatccc acatagattt tcttgaatcg acatccatac
     4621 caacagttgg ttcatctaaa aataggattt taggagatga taccatattc atagctatgt
     4681 ccaatctacg ttttacaccc cctgaatatg atgaagtagg atattttaag taactagata
     4741 agtcaaactt atcaattaaa gagtctattc tttgttttgc aatctgtggt tctactttat
     4801 acattttact ttgaaatacc atattttcca taagggataa atgttcatca atagagattt
     4861 gctgtgctac acaagcaatt tgagtacgta tccaagaagg attatctact aaatcttttc
     4921 caaacatttt tacatttcca gatgtaggtt tgtaaaaggt agtcaaaata tttataagag
     4981 aagatttacc agcaccatta ggaccaagta aagaaaaaat ttctccagca tttacattaa
     5041 agcttagacc atttaaagct ttcacaccat ttttatattc ttttactaaa ttgtctattt
     5101 caatcgctaa catatattat tcctcctctt taagtggaaa tattatctct gtaatatagt
     5161 tatctgggtt tccttttatc aacatacctg gtcctttaat aaatacttct ctccaaggag
     5221 tttgtatttt tatattattt tcatgaatat atttaaatat catttcatac gtttttggca
     5281 aattactata agagcctgtg tgagtaaaaa ataaagcttt tgttttagga aattcttttg
     5341 tagttatacc catacgattt ggagtttcag cagttggaac acataattcc atttctgcaa
     5401 caccagtttt ttggtcaaca tcatagtagc aaaagaatgg tgcaccgtta gactttcctt
     5461 ttatagcttt aaatacattt ggaaaatact ttgttgcctc agtcataggt ccattataac
     5521 gcatggtagc cactgtgact gattcgatat ttttgatttc aattttgcaa ttcatttgtt
     5581 tacctcctaa tgtgtgttca tattcttact agatttttaa gcattaatac atataagatt
     5641 ttgattagtt gaaaacatat aatcttatct cgtttgcttg ttttcaagtc taacataaca
     5701 taaaatatat atcctctcat ttgatgctcc attttgtaaa gctattattt aagctttact
     5761 ttagattttt tagcaaattg aaataaagca atttataaaa taagtattta caaattaagt
     5821 gtattaatcg tataatacac ttaataggat actagataaa gtctgaaata aaggcgtatt
     5881 tttttaaagt aagtgtacta tcgtattaat acactagaat aggaggaatc atgaattttg
     5941 aattagataa tacaactccc atttatttgc aaatagtaaa gtatataaag agacaaatag
     6001 taacaggaga gttgaaacca ggagagacaa taccatcaag aagagaaatg gcacttaatc
     6061 taaaagtaaa cttaaataca gttcaaagag catataagga gatgggtgat atgaatataa
     6121 taaatacttt taaaaattat caaagcagtg taacagttga tgaaaatata ttaaaaaatt
     6181 taaaattaga gcttataaat gaatcactat ctgtttttat agaagatatg aaagcaataa
     6241 atgttagcaa agaagaagtt ttaaaaataa tagaagataa gtattagttt tttaacaaat
     6301 agaaaactta gtttagaaag tttatttata agcatattgg atgtggagga tagtaaatat
     6361 gatagaaata aagaatgtca gcaaaacata taaaaggatg caagggctta aaataagaaa
     6421 gatagaagct ttaaaaaatg taagttttaa tatagaaaag ggaaaaataa ctgcattact
     6481 tggtataaat ggtgttggta aatcaactat gttaaaagca atagcaggac ttataaacat
     6541 tgatagtgga gaaattcgta tagatggaga aaaaataaat gaaaaggtat acaataaatt
     6601 ggcttttgtt ccagatgtac aatctcactt tagcaataca acaataaaag aaacatttga
     6661 atttatggaa attttttatt caaagtggaa taaagaaaaa tcaaaagaaa tgatggacat
     6721 atttaaattg gatgaagatg aaataattga taacttgtca aaaggaaaca ttgcaagagt
     6781 aaaattaata cttggatttt gccaagaccc agagtacata cttcttgatg aaccatttac
     6841 aggaattgat ttgtttaaga gggaagagtt tataggagta atagcgcaat atatggagga
     6901 aaatcaagcc attatcataa ctactcatga gatagtagag atagaaagtt tagttgatga
     6961 ggttgtaatt cttgatgaag ggcagattat aacttcattt aatgcagaag aattaagaga
     7021 aagagaaggt aaatctatat tagataagat gagggaggta tataagaatg aataaacttc
     7081 tatatctaat aaatgtagat ttgagaagaa gcaataagtt ttatatagct tatatatcat
     7141 ttttttcatt aattacactt agtttaaatt tattcgaaat taataaattt aaaaattcta
     7201 aatttattat gaacttagca atagaagatt ttggaggtat attttatgga ctagggatac
     7261 ttagtaatct tggttttatc caaaatatat tgttctttgg aatgttggga ctttttatat
     7321 actatatttt tatgtggaaa agagagttct tttcaaataa tcaaagtatt tatacactta
     7381 tgatgttacc tcaaaataag tttaagattt gtatatctaa atcaatagtt ttgataacaa
     7441 tgatatatgg ttttttgata agtcagacag caacattatt tatatgtaaa tatatattta
     7501 atttcatttt tagaaatatt cctataatca atatgaactt tgccaaagat ttatattatt
     7561 taaacttgaa gagtatacca attgatttta tctcttttat ggctatttat gtatttttac
     7621 tacttatagc tatttctatt gtaaattgct gtatattatt tatcttttcc tatataaata
     7681 aattttatgt aattttaatc actttgctga ttatagcagg cttatatttt tcaacaatat
     7741 atataaatat aatca
//
LOCUS       DAEPOG010000168         7742 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEPOG010000168 DAEPOG010000000
VERSION     DAEPOG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.168"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            84..632
                     /locus_tag="KR432_003068"
     CDS             84..632
                     /locus_tag="KR432_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0641583.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            751..942
                     /locus_tag="KR432_003069"
     CDS             751..942
                     /locus_tag="KR432_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641584.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1165..1515
                     /locus_tag="KR432_003070"
     CDS             1165..1515
                     /locus_tag="KR432_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641585.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            1777..3282
                     /locus_tag="KR432_003071"
     CDS             1777..3282
                     /locus_tag="KR432_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBH0641586.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            3672..4769
                     /gene="dinB"
                     /locus_tag="KR432_003072"
     CDS             3672..4769
                     /gene="dinB"
                     /locus_tag="KR432_003072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH0641587.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            5025..6161
                     /locus_tag="KR432_003073"
     CDS             5025..6161
                     /locus_tag="KR432_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641588.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6154..7701
                     /locus_tag="KR432_003074"
     CDS             6154..7701
                     /locus_tag="KR432_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0641589.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
BASE COUNT         3002 a          830 c         1385 g         2525 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatagg aggttcagtt
       61 taacccattt agataaggac catataggtg gagtagatag tgtgttatct agtgttaaag
      121 taaagaatat tatacaagca aattataaga aagatagtaa gcaatatgat gaatatattg
      181 actcacttaa aaaagctgat atagaaccag tgctactaaa agataatatg aatattgtaa
      241 tcaatagtgc agaaataaac atacatccgg cttcaaaaag caagtacgag agttcaaatg
      301 attattcaat aatcactaat ataagctatg gagcacataa gtttttattt gcaggagatg
      361 cagaggaaga aagattgtca gagtttataa atggaaatac tttaaaatat gattttgtta
      421 aaatgcctca tcatggaaga tatgacaaat taactgaaac atttttagag tctacttcac
      481 cccaatatgc tgttattaca tgttcagaga aaaaagaacc agaggaaggt gttttaaaaa
      541 tattggaaag attgaatata aaaacatttt tgacttctag tggagaggta gttatcaata
      601 gtgatggtaa gacattgagt gtgaatcaat aatttttttt aaaatatcta aaaaaaattt
      661 aaaaaaatca ttagcaaata ttacccctta aacatagtat attttaggag ctgaaactcc
      721 acatacaaaa tgactaggag gtaacatatt atgttggata gattttttgg tgaaggcgga
      781 tttggtggat gtgcttggtg gataataata ttattcttct tattcttagc attccaagat
      841 tgttgggctg aaatggactt atgtgcatgg atacctttct taatattatt attaatactt
      901 tgtgaatgtg gtgacgtttt tgatggagga tgtggatgct aaaaaaatca atttagatga
      961 tttaataaag cttatggtgg gtataaataa aataccaaac caatcacaat ctagtgtttg
     1021 aaggtagtcg gaaatccccc gctaccttct tttttttatt gttagaattt atttttgaat
     1081 tatttcattt acatataata atttttacaa taaagtctga tataattaaa aagaaaataa
     1141 aatttattta taggagatgt aaaaatggct atagtggata agattttcaa tgagataaaa
     1201 cttatggttg caagactttt attgggaaaa aaatacagtg aatatgaaga aaaagattta
     1261 gtgtataata cagaagaaga agttatactt ataactttaa aaagattagt ttttcaggga
     1321 aatgtaaacg aagcagaaga aattttattt gataaagcta agactgtaac tagtgaaaat
     1381 atgcagtata tagcaataga attttatact atgcttatgg aaaagactga tgaagaatta
     1441 gaagctatga atttttctaa acaagaagtt tatcaaggta tagaagatat tagaaaagta
     1501 ttgaatttag aataaaagat atatgaaaat attgaaataa attaatgtta aggtgagaat
     1561 attatactta tatataaaaa tataatattc ttattttatt agttatatta ggtatatctt
     1621 atttaccata aaactatctt gaaaaaaatt aatcttaatt gtataatatt actttgagtg
     1681 aaaaagtatt aacatttttt tataagatgt tttactaaaa aaatatatat aaatatactg
     1741 ttatcaacaa gaaatttaat ttagtgaggt gttaagatga aagtaggatt tgaccacaaa
     1801 aaatatttag aagagcagtc taaatacatc ttagaaagag taaataattt tgacaaactt
     1861 tatttagagt ttggaggaaa attgatggga gaccttcatg caaaaagagt acttccagga
     1921 tttgatgaaa atgcaaagat aaaagtatta caacatatga aagaaaaagt tgaagttgta
     1981 atttgtgtct atgcaggaga tatagaaaga aacaaaataa gaggagactt tggtatcact
     2041 tatgatatgg aagttttaag attaatagat gatttaagag gttatgaact agatgtaaat
     2101 agtgtagtaa ttactagatt tgaaggacaa cctgctacaa ctgtatttat aaataaatta
     2161 gaacgtagag gaattaaagt ttataagcat tatccaacaa agggatatcc gtcagatgtt
     2221 gagactatag ttagtgatga gggatatgga gcaaatgaat atatagaaac tacaaagcca
     2281 atagtagtag tcacagcacc aggacctaat agtggaaaac ttggtacttg tctaagtcag
     2341 ttgtatcatg aaaataaaag aggtaatgta gttggatatt ctaagtttga gacattccca
     2401 gtttggaatg tacctgtaaa acatcctttg aatatagctt atgaagcagc tacagttgat
     2461 ttaaaagatg tgaatatgat agattcattc caccttgaaa cgtatggtga aatgtcagta
     2521 aactacaata gagatttgga attatttcca gtgcttaaaa aaataataga aaagataact
     2581 ggaaaagaat caattttcca atctccaact gatatgggtg ttaatagagt tggctttgga
     2641 ataatagatg atgaagtaat aagaaaagct tctatagaag aaataataag aagatacttt
     2701 aaaactgcct gtgagtataa aaagggacaa gttgataaag gcgcttatga tagaatgaaa
     2761 atgattatgg aagagcttaa cttaaagcca gaagatagaa atgttgtatt acctgctaga
     2821 aattacagta ataaattaaa agaaagtgcc gacaagaatg atacttgtcc tgtagttgca
     2881 ttagaattag aagatggtac aatattaact ggtaagggtt ctgatttaat gaatggaaca
     2941 gcagcagtag tattaaatgc cattaaacat ttagccaata tatctgatga tatgcactta
     3001 atatcacctg taatattaga acctatcata aacttaaaaa caaagacttt agctagtaga
     3061 aatgtttcac ttagttgcca agaggtgtta actgcactta gtatttgtgc tgtaactaat
     3121 ccaactgcac agtttgctat ggaaaagtta gcattattaa aagggtgtca agctcattct
     3181 actactatat taaatagaga tgatgaacaa acatttagaa agcttggtat agatgtaact
     3241 tgtgacccag aatatccttc acaaaatctt tactatagtt aaaattatat aaaaaaatat
     3301 gggctgtttt aaagtaatag tttgagcagt ccattacaat tataataaaa tcagtaggct
     3361 gtcttgtata agacagtctt ttgtttttaa aacatcccat aaattaaata tatatactgt
     3421 tatccataag gaattttaaa tttagttcct tggaatacct attgtaaagc ttttatatag
     3481 tttttgcaag tgtttttaag gattttttat aaattatatt gaagttaatg gattatagat
     3541 ggttttattt atggaaaata tgaatatata aagaaaataa tgaaaaaata ccaaaaagaa
     3601 acataagttt ggtataatta aaaagtaata taaataaaat taatctattt atactttaaa
     3661 aggtgataat aatgaaacat tatagaaaaa taattcatat agatatggac gctttttatg
     3721 catctataga acaaagagat aataaaaaat taaaaggaaa acctgttatt gtcggtggaa
     3781 atccacaaag ccgaggtgtg gttgcaacgt gttcttatga agctagaaaa tttggtatcc
     3841 attcagctat gccatcagct gtagcttaca ataggtgtcc atatgcagtt tttgtaagac
     3901 ctagatttga tgtttataag agtgtgtcag agcaaataag agatatattt tatagataca
     3961 cggatttagt tgaaccatta tctttagatg aagcttactt agatgtaaca aaaaataaaa
     4021 aaaatattga ttcatcaatt gagatagcaa agcaaataaa aaaagatata tttagagaag
     4081 ttggtcttac ttcttcagct ggtgtgtcat acaataagtt tcttgctaag attgcatcag
     4141 acttaagaaa gccaaatggg ttaactgtaa taaccgaaga aaatgcccag gactttttag
     4201 ataaattacc tgtgaataaa ttttttggtg ttggaaaagt tacttcaaat actcttaaaa
     4261 atttaggtat aaaaactgga tatgatttaa ggtgtttaaa tttatttgaa cttgaaaata
     4321 tattcaaaaa aagaggttat gaattatata aatttgctag aggaatagac gatagaccag
     4381 tggaaccaaa tagggttaga aaatctgttg gggctgagac tacattaagt cataatttgg
     4441 atattgatga agaagaaaca cgcaacattc ttgatgaact ttgtgaagaa gtatgtcata
     4501 gattaaaaag ctctgagaag tttggaaaaa cattaacatt aaaaattaaa tatgaggact
     4561 ttacaaagat aactagaagc cttagtttag aacactatat tgacgaatat aatgatataa
     4621 gaagtggtgt agataatttg ttgagaaatg ttgaggttaa tggaaaacaa attaggctat
     4681 tgggagttac aatatcaaac ttaagtgata aaaaagaaac ttataaagat attacattat
     4741 ttgaatacat ggatagtatt caaatgtgag ttttgtttaa atgaaataac actagcttta
     4801 atactatata attgaggcta gtgttatttt atgaatattt ttatactttt tgttttttga
     4861 cattatatac tatatataaa tactttatat ttgtagcaat attcagacaa atagtgacct
     4921 aaaaatcgta ctttgtttac ataaataaaa tagtatgata taattataaa aacaacttat
     4981 aatattggag aaggttgctt gttcgaaaaa ggggatgata aattatgata gagataagaa
     5041 atgtaacaaa aaaaatagga aataatgtta tattagatga tatttctcta gttgtagaaa
     5101 caggtacatt ggtagttttg attggttcaa gtggttgtgg aaagactact actttgaagc
     5161 ttataaacaa attaataaaa cctacatctg gtgagatata tattaatggc aaaccaattt
     5221 ctcaggaaaa tgaaatagag cttagaagaa aaataggata tgttatacag aacacaggcc
     5281 tattccctca tcttacaata aaagaaaata tagagctaat tccacgccta aaaaaggaaa
     5341 aatccgttga agaaattgaa aaaagaacgt tgcaactact tgagatggta ggacttgata
     5401 gtgatgaatt tttgaataag tatccttcag aacttagtgg tggacagcaa caaagaattg
     5461 gtgttgcaag agccattgct actgatgctg aaataatatt gatggatgag ccttttagtg
     5521 ctttagaccc aataacgaga acatctttac aagaacaatt attttctctt caagatgaat
     5581 tgaaaaaaac tataatattt gtaacgcatg atatggatga ggctttaaag atagctgata
     5641 aaatttgtat tatgaaggat ggaagaatag cacaatatga tactcccgaa aatatactta
     5701 gaaagcctgc aaatgacttt gtaaaagatt ttatcggaga agatagagtt tgggataatc
     5761 cagaatatat aaaagcaaga gatattatga taaaaaatcc tattgctgtt aattccacta
     5821 gaactgtaac tcaaggaata gaaattatga gaacgagtaa ggttgatagc ttactaatta
     5881 tagatagagc aaaaacatta aaaggtattg ttactgtaaa agatatgaaa gatattgatg
     5941 ataagtcaat attacttgct gatattatgt cgagtgagcc tttgcatgta aatgaaggtg
     6001 ataatttagt agaaatacta aatgtaatga atcgtaattc ggttggatat attccggtta
     6061 tttctgatga aaataaactg gtagggctta ttactagaag tagtttactt tctgttttaa
     6121 gtgaacaatt cttagaaatg gaggtgagtg ttcttggcta atttttttaa ctttatacta
     6181 ttacaaaaag ataagattat agagctttta atacaacata tgagtctaac tgttacatct
     6241 attttaatag ctataatagt tggtgttcct cttggaataa ttatttcaag aatctcatct
     6301 cttagaaagt ttgtacttgg atttgtaaac ttagtccaag cagttccgtc tatggcactt
     6361 ttgggtcttc ttgtacctat acttggcata ggaagtaaac cagccatatt tatggtagtg
     6421 gtttattcat tactaccaat tgtaaaaaat acgtatatag gtataacaag tattgaccca
     6481 gtagtattag aatcagctaa aggtattggt cttacaagaa atcagacatt atttaaaata
     6541 caattccctt tagcacttcc tataataatg ggtggagtta gaatatctgc agttacagct
     6601 gttggtttga tgactcttgc agcatttata ggtgctggtg gtttaggata tttagtattt
     6661 tcaggggttc aaactgtaaa taataatatg attttagcag gggcaatccc atcatgtata
     6721 attgcactgt tagttgatta tttatttagt aaaattgaag ttgcagttac tccaaaagga
     6781 ttaaatccaa aagcacctaa gaaaaattat atagcactta aagtaatatc tgtagtggtt
     6841 gttgtttcaa tgttgttcgt tgtttttagt tctagttttt caagtaaaaa agatacaata
     6901 actattggtt ctaaagatta tacagaacaa cttatacttg gtaatgttta tgctgaatta
     6961 gtagaaaaaa atacgaatct taaagttaag aagaatttaa atctaggtgg ttcatctgtt
     7021 gcgtttaatg caattaaatc tggtgagtta gacatgtatg ttgactatac aggtacactt
     7081 ttggtaaatg taatgaaaca tgctccgatt aaagatgctg atgaagcata taatgttgta
     7141 aaagatacta tggaaaaaga aaatcagtta actttacttg accctcttgg atttaataat
     7201 acttacacat tagctatgat gcctgaaact gctgaaaagt atggaataaa tactatttct
     7261 gatttgacta aatatggtaa agaatttaca ttctcaccaa ctttggagtt tgaaaataga
     7321 gaagatggtc ttgtagggct ttctagagat tatggtttaa aattcaaaga tgtaaaagca
     7381 atgaatggaa gtttaagata tacagcttta gataataatg aatctcaggt tatagatgca
     7441 tttttaactg atggtcttat taaaaagttt aatcttaaga tattagagga cgataaaaat
     7501 ttctttgtac catattatgc agcaccatta gtaagagaag atactttgaa gaaatatcct
     7561 gagttagaaa aagttatgaa tatgctttca ggaaaaataa atgaagaaac tatgagagaa
     7621 cttaactatc aagtggatga acttggtaaa tcaccagaag aagttgctca ttcattctta
     7681 gttaaagaag gcttagtata aaaaattaat agtttatatc aaaaatttaa gggagtgtct
     7741 ca
//
LOCUS       DAEPOG010000169         7651 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEPOG010000169 DAEPOG010000000
VERSION     DAEPOG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.169"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            162..1028
                     /locus_tag="KR432_003075"
     CDS             162..1028
                     /locus_tag="KR432_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0641590.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            1169..2311
                     /locus_tag="KR432_003076"
     CDS             1169..2311
                     /locus_tag="KR432_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0641591.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            2695..3933
                     /locus_tag="KR432_003077"
     CDS             2695..3933
                     /locus_tag="KR432_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641592.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            3962..4849
                     /locus_tag="KR432_003078"
     CDS             3962..4849
                     /locus_tag="KR432_003078"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBH0641593.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            4883..5512
                     /locus_tag="KR432_003079"
     CDS             4883..5512
                     /locus_tag="KR432_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0641594.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            5517..5981
                     /locus_tag="KR432_003080"
     CDS             5517..5981
                     /locus_tag="KR432_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBH0641595.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            6231..7598
                     /locus_tag="KR432_003081"
     CDS             6231..7598
                     /locus_tag="KR432_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0641596.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
BASE COUNT         2854 a          780 c         1273 g         2744 t
ORIGIN      
        1 cctacgattt ttatttataa ttaagtttca aatagaatga acaagtacaa ccaattttga
       61 atataaatat agtaaatgtg cttatatacg attttattat ctgggtgatt gatattgata
      121 gatagctttc tttattgaat cgaagggagg gggaatttgt tatggataac tataagaaag
      181 tgaagcaagt attgtggata attttatttg ctaattttgc agtagcatta ttaaaaatca
      241 ttattggtaa tcaaataaaa agctatagta tgacagcaga cggatttcat tctttgtcag
      301 atggagcatc taatatagtg gggcttatag gtattttctt tgcatctaaa ccaaaagata
      361 aaaatcatcc ttatggacat aaaaagtttg aaattataac cagtttattt ataagtggta
      421 tgttgtttgt tatagcaatt aagataattc ttagtgcagt gctaagaata gctaatccag
      481 ttgtacctgc tataactata gaaagtttaa ttgctttaat tattacactt tttataaata
      541 tttttgtatg catgtatgaa tatagaattg ggactaaact aaatagttat gttttaattt
      601 cggattcatt gcatacaaga agtgatatat ttgtgtctct aggtgtattg gttactttag
      661 ttggtgttaa gttaggattt ccagtaataa tagaatctat agttcctatt attatttctg
      721 cattcataat ttattcggct tatggaatat ttaggccatc tattggaatt ttggtcgata
      781 gagtggctgt agatgaggat tatataaaag aaattgtgtt tgaatttaat gaggtaaggg
      841 atgttcacaa tatcagaagt agaggtagca aaagttctat ttatattgac atgcatgtaa
      901 tggtagaccc atttataagc gtagagcaat ctcatgattt aacacataaa attgagaaac
      961 aaatacaaga agaaattaat gaaaatgctc aagtaatagt ccatattgaa cctttttata
     1021 gtttttaact tagctataaa atacaagtta taaaaaaata taatttataa aaaaaatatt
     1081 ataccataaa taatttataa aaattattta tggtataata ttttctataa agaaattaag
     1141 tcttaatata tctaaggagg aatttacaat gagatacgtt gaatatggaa agacagggaa
     1201 aatggtttca gtagttggct atggaggatt aaggtttgat ttagaaaaaa gtaatcaaga
     1261 aaatgctgat ttagtcaaat atgcgtatga aaaaggtatt aattactttg atacagcgcc
     1321 agggtattgt gatgacagaa gtgaagatat atttggacta gcctttagac aaatgataaa
     1381 ggaaggtaag accgattttt atgtatcaac taaagaaaag ccaaaagcat gtgatacatc
     1441 agaaaaagct atagaggctg taaaaaaatc aatagaacga ttaggcgtat ctaaaataaa
     1501 tttttatcat gtttggaata ttagaaagat ggaacattac gaactagcaa tgagacctgg
     1561 aggtcaatat gaaggattat tgaaatgtaa agaagaagga ttgatagacc atatagtatt
     1621 ttcatctcat caaccaggag atgaggttat agatattcta agtgaaaata agtttgaagg
     1681 tgtaactatg ggaataaata tattaaactt tccatatagg ctaaaaggtg ccaaatatgc
     1741 tgttgataat ggatatggtg ttgtggcaat gaatccatta agtggtggaa ctattccaaa
     1801 gtataataaa gaactttctt ttctatcaaa agatggcgaa agtactatag aatcagcttt
     1861 aagattcaac ataggaattt cacaaataag tatatctttg atagggttta ataaaaaaca
     1921 agatatagat gatgcatgta aaattgcaga cgaaaataga atttattcag atgaggatat
     1981 acatgatatt gagactagat taaataagaa tatgaacgaa atatgtacag gttgtggtta
     2041 ttgtaaagta tgtccaaaag gtattaatac cccagcctat atgctttttt ataatgaaaa
     2101 gcagatgttt aaaaagagtg atgaagaaat gacaaagtta gtttatggtt taggacattg
     2161 gaattacaca atgaatagta aggcaaaagc taaagaatgt atatcttgtg gaaaatgtga
     2221 agttgaatgt actcagcatt tacctataat agatagatta aaagaaatta aaaaatggga
     2281 agaagatgga gctaatagtg ttaaagtcta attaatttta tactatattt gaaatttgta
     2341 tagtatttag taatgtttta aaatatagtt ttaatattta aggagacaga ttataatttt
     2401 gtctccttaa attatgcttt aatttgttat agtttaattc cataagcaac attgtatatt
     2461 tttatataaa gtttttcaaa tattttacat taatattgct tacaaattct cattttatac
     2521 aacactaatc tatgttatat aaacaatact tgactgatta taacatcaat aaagtaaata
     2581 ttgtggttgt aaataaagag ttattcaatt acaataaaac aagtaagaac attatgaaga
     2641 tggctattaa atataaggaa taaattgtag gtgtcagcaa atcctatata atgaatggat
     2701 ggtgatatat tgatgagtaa aatattaaaa tataaaaacg agatgttttt gtttatatta
     2761 gttgtaataa cttacttaat aataactaag atattttttt ctaagactac tatattttat
     2821 aatttaaaca atacttatga tgtattgtta gatacagata caggggtttt attcaattta
     2881 aatgtatttg ctataagtca agacaatagt aagcacatat tgttttcagc aattatatca
     2941 atttttgcat atccaatata cctattttgt acttcaatag caaatcctgg gacaacagat
     3001 tttaatagtg cctatgggtt tggactaatt tgtctacaaa taataactag tgctatgtca
     3061 ataactttag tctttaatca tattaaaaag ataaaaatgc agagattaac tttaatttta
     3121 cttactatga taatgatatt ttcttttcca cagttattta tgactttaaa tgtagagaga
     3181 tttatatata gccaattttc attaatattt ttcatagtta ttgcaaataa gatgaaagga
     3241 aaaaattctt atttagtaga attagcagct attcctctat ttggaataac tattagtaat
     3301 atatatttgt atttttttaa tatgatattt gagtttaaat taaaaatagg aaaaatgtta
     3361 aaacatttaa ttacatttat cctaatggca tatatttgtg tagtttcaac gaagtcttat
     3421 gagagtttta tgaaccttgg aaatgtaata cagtatgata cgaaatttat aagtggagaa
     3481 ccaattttgg aaaagattgc aatgattata gagagattat tgtattcggt tttttatttt
     3541 cctggagcaa gtattaaaaa agggttattt ttgcaaaatg gagaagttgc tacaatacca
     3601 gtaattctta cattattagc tttatgtttt tgtgttttat cagttattga aaattcagaa
     3661 aaaagagttc ctaagctatg tatgggaata attatattca atctaacttt acatggtata
     3721 gtaggatata atctagtcaa ttcaagtatt atggctataa attttagttt tgcagttata
     3781 attttattgg cttactttac gaaagcgctt agaaaaaatg aaaagaatat gtataatata
     3841 tttctatcgc tgttattggt tactatagta atcagtaatg tcaatggatt tatagaaatt
     3901 ttaaacatag ggataaaatc atatcctgtt taaaaaataa tattgaaagg gtagtaagtt
     3961 tatgaaacag accaatacaa gtataaggct tcttactaaa atttcagatt atataaagtt
     4021 aatgagaata aagcaatata taaagaattt ctttgtattt tcagctataa ttttttcaaa
     4081 caacatacta aatataaatt tatttttaaa tacgtttatt gcgtttgtgt gtttttgcct
     4141 aatgtcatca tcagtatatg cactgaatga tgctatagac atggataaag ataaaaaaca
     4201 tcctaaaaaa tgtaatagac cagtggcaag tggaagaata agcaaaaaaa gtgctaatat
     4261 actatttgta gtattagctt taatttcagt ctgtttatca acagttgtta gttttaatct
     4321 ttctataatt cttagtctct atttgataaa caatatatta tattctctta aactaaagaa
     4381 tataatacta ttagatgtgt tttcaatatc tcttggtttt atacttaggg tttatgcagg
     4441 atgtgtagcc ataagtgtta gtttatcaaa ttggataatt ttatgtacat ttttcttatc
     4501 tctgtactta gctcttggaa aacgtaaaaa agaaatagaa actctaagag atgatgcagt
     4561 tgaacataga aaaatattgg aagattatga catagaaaat ctaaatcaaa tgatgatagt
     4621 tatattaagt tctacaattg tatgttattc attgtattct acaagtaatc cagaaaagcc
     4681 acatatgata tttacaacaa tatttgtagt ttatggtgta cttagatata attatataat
     4741 taacactaca aatgaaaata atcctactga tattgtccta aaagataatg ctttaaaaat
     4801 aaacgtaatc ttatggataa ttacatgttt aataatattg attttttagt gatattttta
     4861 gtgataggat ggggaaatac ttatgaaaaa aagtgtatta ttacttgatg tagattatac
     4921 tgttataaat actgatagca tgatagattt ttttatttac tctttaaaaa ataaaacctt
     4981 taaaactatt ataaagttgc catacattat ttttattcta tttatgtatt taattagaat
     5041 gatacccttg aagaaggcaa aagaggcaat cttttatcca atagtagatt ttagtgaaga
     5101 agatttaaaa aagttttttg atgattgcat aatgaaaaaa attaacgaat ctatgaaaaa
     5161 ggtcatttac aaaaataaag aagaagataa tgttataatt atgataactg catctccata
     5221 tgcatatatg aaatatttta agtattatgg tcttgcagac aaggttatag gcacggagtt
     5281 tttttatgaa aattcaagat ataaaaatca attcattgga gaaaactgta agggaattga
     5341 aaaagtaaaa agaataaagg ctgtattagg taaacttggg atagagattg attatgaaaa
     5401 ttcatatgca tattcagact ctaagagtga tttaccaatg ctttcattga ctaaaaatgc
     5461 atttttagtt agtaaaaagg atggtgaggt tatagagcaa ataaattcat aacttaatgt
     5521 cacttagaat aggagggaat agtttgggat atttacacat attaatttta tctatggttc
     5581 ctgtaacaga attgagggga gccattccta taggtattgc aatgggctta aatcctatat
     5641 gggtttatgt atttagtgta ataggttcta cattggtatc gattcccttg atattgacat
     5701 tcagacatgt attacaattt ttaagaggga agaaactatt taaagggatt gctgatgtaa
     5761 ttgatagaaa aataaactca agaatgaaga aattaaaaag tgtttcgata attggtataa
     5821 tactatttgt aggtatacca ctgccaacta caggaacatg gactgcatct gctatagctt
     5881 ccatattaaa aatgagaata aaagatgctt ttatgggagt atttcttggc aatttgttat
     5941 caggggttat agtttcagct ctttctttac atataatata aaactatcta agtaatatac
     6001 ttggatagtt tttttatgag tacatgtgaa aaatttttaa taataaataa aaaatgagat
     6061 aaagtattta aattcagtta gttctaaaat gtttactatg gcaaaagaaa tatagtatag
     6121 tattacttgt agggaaagta ctctaaattt gtctgctgat aaaatgaaat ttttatatag
     6181 aataaaattt tagtgataca ttttaattat tggattgagg aggttttaaa atggaaaatt
     6241 caaacactaa cttaggcagt gaaagtgtgg gtaaattatt atttaaactt gctacacctg
     6301 ctattattgc acaaattgta aatgttttat acaacatagt cgataggatt tttattggta
     6361 gaatggaaaa tggagaagtt gctatggctg gtgttggagt ggcctttcca ataataatta
     6421 taataactgc atgtagttat ttaatcggta tgggaggagg tccattagct gctataaaaa
     6481 tgggtgagca aaacaatgat gaggcagaga aaataatgag taacagtttt tctgtactgg
     6541 tgattttagc aatacttttg actataggtt ttaagattgg aaaagagcct ttactttgga
     6601 tgtttggagc aagtgaatct acaattagat attcaatgga ctatttaaat atttatttaa
     6661 taggtacagt gtttgttcag atttcaatgg gtatgaatac ttttataaat acccaagggt
     6721 ttgctacaac tggaatgatg acagttgcta taggtgcttt aataaacata atacttgacc
     6781 cgatttttat atttggcttt aatatgggtg taaaaggagc tgcattagct actataattg
     6841 cacaaggcgt ttctgctatt tgggtactta tgtttctctt tggaaagaaa agtatactta
     6901 aaataaaaaa gaagtatatg ataccaaaag caagtatcat cttaccagtg cttggcttgg
     6961 gaatctctcc atttataatg caatctacag aaagtttagt acttatagcc ttaaatagta
     7021 aattacaaat gtatggagga gatttagcag taggttcaat ggccataatg agcagtataa
     7081 tgcaaattct tatgcttcct aatatggggg ttacacaagg agcacaacca ataataagtt
     7141 ataattatgg ttcaggtcaa cttgatagag taaaaaagac atttaagttg tgtttattgt
     7201 catgttttac atattctact attttatggc ttttattaat gatatttcct gcattttttg
     7261 ttagtatatt taataagaat ccgcagttac ttagtatgac tagttggagt ataaaaatat
     7321 attttgctgg tgcttttatg tttgggattc aaatagcttg tcaacagaca tttttagcac
     7381 ttggaaaagc aacaatatct ttggttttag ctctacttag aaagattgta cttttaatac
     7441 ctttaatatt tatactccca acattcttta atgaaaaact ttttgcagta attttagcag
     7501 agccagttgc agatataaca gctgctacta taacagcaat ttctttcttt atattttata
     7561 aatgttttct atcaaaacct aaagcaataa aagaataaga tatacaatat aaatttttta
     7621 tgaaaatatt ttattagata aaaattagaa t
//
LOCUS       DAEPOG010000170         7635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEPOG010000170 DAEPOG010000000
VERSION     DAEPOG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.170"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            130..300
                     /locus_tag="KR432_003082"
     CDS             130..300
                     /locus_tag="KR432_003082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641597.1"
                     /translation="MYLDTLFFRMKFETIELDLSKSGSTAKVAETKISTKDIGILNIK
                     DVWIAVKIPIAQ"
     gene            531..2207
                     /locus_tag="KR432_003083"
     CDS             531..2207
                     /locus_tag="KR432_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBH0641598.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            2413..2613
                     /locus_tag="KR432_003084"
     CDS             2413..2613
                     /locus_tag="KR432_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBH0641599.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            2667..3830
                     /locus_tag="KR432_003085"
     CDS             2667..3830
                     /locus_tag="KR432_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0641600.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(4041..4463)
                     /locus_tag="KR432_003086"
     CDS             complement(4041..4463)
                     /locus_tag="KR432_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBH0641601.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(4484..5320)
                     /locus_tag="KR432_003087"
     CDS             complement(4484..5320)
                     /locus_tag="KR432_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0641602.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            5618..6214
                     /locus_tag="KR432_003088"
     CDS             5618..6214
                     /locus_tag="KR432_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBH0641603.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            6244..6963
                     /locus_tag="KR432_003089"
     CDS             6244..6963
                     /locus_tag="KR432_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBH0641604.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            6965..7504
                     /gene="scpB"
                     /locus_tag="KR432_003090"
     CDS             6965..7504
                     /gene="scpB"
                     /locus_tag="KR432_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBH0641605.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT         2983 a          863 c         1261 g         2528 t
ORIGIN      
        1 tgggttagtt ttgcttcaat aattataatt gcaacaaatg taattattgc gcttaaataa
       61 aaattatcat ttatatttat tgaaataaag aaaataataa tattaacagc atatataaaa
      121 attctatata tgtatttgga tacattgttt tttagaatga aatttgagac catagaatta
      181 gatttatcca aatcgggtag tacagcaaaa gtagctgaaa ctaaaatatc aactaaagat
      241 ataggaattt taaatattaa agatgtttgg atagctgtta agattcctat tgcacaatga
      301 gttttaccac gcataaatcc tcctaaatta tccgatatta aatcatataa tatttagagg
      361 atatcaaaat aaaataggtt tgtcaaaata atacgttaaa tgtattttaa atatagctta
      421 aaatatgata cgaattatgg tacaataaga ataactggta gcgagtgcgt gatttgatat
      481 tatttatgat tgtagaacgt gttaaaaaat taaatagaca gaaggagatg atgcaattgt
      541 tcaaaaagaa caccaacaag ataaaggtaa tggccttggg tggacttaac gaagttggaa
      601 aaaatatgac tgttgttgag tataaagatg aaataattgt tatagatgct ggacttagtt
      661 ttccagagga tgaaatgtta ggagtagata tagttatacc agatataact tatttagtaa
      721 aaaataggga taaaataaag ggtatattta ttacacatgg acatgaagac catataggag
      781 ctcttccata tatattaaag aaaataaatg tacctgtata tggggcaaga cttagtatag
      841 gtcttataca agtaaaactt aaagaacata aaatgaataa tgttaagtta aatgttatag
      901 gtcctagaca agttataaag cttgataata tggaagtaga gtttttgaaa aataaccata
      961 gtataccaga tgcttactct attgcaatac acacagacca aggtataata taccacacag
     1021 gagattttaa gattgacttg actcctattg atggagatgt tatggatatg catagaatct
     1081 gtgagttgag caaaaaagga gttcttttaa tgctggcaga tagtactaat gcagaaaaac
     1141 caggatttac tatgtctgaa aagactgttg gggtaggtct tgacgaatta tttgcaaaag
     1201 gaaatggcag aagaattata gttgctacct ttgcatccaa catacacaga ttacaacaaa
     1261 taataaatac tgctgaaaag tttaatagaa aagttgctat atcaggtcgc tcaatggtca
     1321 atgtagtcgg agttgcaaaa gaattaggat acctagatat ttctgatgac atgcttatag
     1381 atttaaatga tatatgtaag tatgaagata gtgaattagt gataataaca actggttcac
     1441 aaggtgaacc tatgtcagct cttgctagaa tggctttttc agagcacaaa aaagtagaaa
     1501 taaagagtgg cgatttagtt ataatatctg ctcatccaat accaggaaat gaaaaattaa
     1561 tatcaagagt aatcaatttc ttatttgaaa aaggagcaga agtagtatac agtgatatag
     1621 ctgatataca cgtttctgga catgcctgtc aagaagaatt aaaacttata catgcattag
     1681 ttagaccaaa gttttttatg ccagctcatg gtgaatatag aatgttaaaa agacatgctg
     1741 aaatagctga acagcttggt atggataaag aaaatatttt tgtcatgcaa acaggagatg
     1801 ttttagaatt agataaaaac tctgctaagg tagcaaaccg tatacaaaca ggaaacatat
     1861 tagttgatgg tctaggagta ggcgatgtag gtaatatagt tttaagggat agaaagcatt
     1921 tatcagaaga tggtcttatg atagttgtag ttactatttc caaggatgag ggtaaggtct
     1981 tagcaggtcc agatataatt tccagaggat tcgtatatgt aagagagtca gaagatttaa
     2041 tggatggagc aaaggatata attaagaatg ttctaaatga atgtgaagag aaaaatatta
     2101 aagaatgggc ttatttaaag aataatatta aagaaaacct aaaagaatat ttatatcaaa
     2161 aaactaaacg aaatccaatg atacttccta tcataatgga agtataatat aaaaaaacta
     2221 taaatttgaa aattagacct cttgtaattt ttaaagtaat atcaaaaatc caattgatat
     2281 tatcttaaga attacaagag gtctttttaa tactcattta aaaaaaatat atattacata
     2341 aaataccaat tataacaatc ttttattagt aaaaactact tatatataaa ttttactgat
     2401 aggaggattc ttatggatga tgtttcaaga caaaatgcta taaaagcttt aaaacaaaca
     2461 aaaatggaaa tagcaggaga gtatggaatg aattatgaag atgcttttga aataatagaa
     2521 aatgcgtcta ataaaggtgt tttagaaggt tactttaaaa agcttgaaaa gaaaaaaaat
     2581 ttaggtcaag gtatatctag gcatttagaa taaaaaaagt taaatgtgag aaaataagtt
     2641 taaataaact ttaagaggtg acatcgatga aaaaattagc tagtactgct ttggctatat
     2701 taattgcgct tactccttta agcttttctt ttgcaaataa taaggaaaat gctgatgcca
     2761 accaactaaa tatatcttca aaatcagcaa ttttgatgga tgttggtagt ggacaaattt
     2821 tatatgaaaa ggatgctcat aaaaagcttc ctcctgctag tgtaactaag gtaatgacta
     2881 tgctacttat agtagaagct ttggattcag gcaagattaa gcttgatgat gaagttcaag
     2941 taagtgaaac tgcatctagt atgggtggta gtcaaatatt cctagagcca ggagaaactc
     3001 aaaaggttga tacattatta aaaggtatag ctgttgcttc tgcaaatgat gcttgtgttg
     3061 ctatggctga acacttagct ggaagtgttg aaggatttgt tgatagaatg aatgctaaag
     3121 caaaagagtt aaatatgaat gatactcatt ttgcaaatac aaatggactt ccagttgcaa
     3181 accactatac atctgctcat gatatagctt taatgtctag agaactatta aaacacgaaa
     3241 tgataagtaa atacttaact acatggatgg ataaggttgt agtagggaag aaacaagtta
     3301 cagtaggtct tgcaaatact aataaactta taaagcatta tcaaggagca actggtgtta
     3361 aaactggttt tactcaagaa gcaaaatact gtttatcagc atctgcaaag agaggtaata
     3421 ctcatctagt tgctgcaact ttaggagcag agacttctcc agaaagattt aatgatgcct
     3481 ctagcctttt aaactatgga tttgctaatt atgaaagtgt taagttatgt agtaaaggtg
     3541 ataatatagc aacacttaca ttggataagg ctgatgaaaa caaagttaaa ttggttgcta
     3601 aagaagattt aaatgctctt attaaaaaag gaagctctaa agaatttgag aagaaaattg
     3661 aaatagtaaa aaatcctaag atgccaatta aaaagggaac tgttttagga aaaataaaga
     3721 tatgtaaaga taaaaaagta ataggcgaag tggaattgat aaatactaag gatataaata
     3781 aggcaagcta tctacaaatg cttcaaagaa taattgataa tatgatataa ataattatca
     3841 gatgaaatat aattataata cacaaagata tgattataat acacataaaa aataccctta
     3901 tgtagttatt tgccataggg gtatttttat ttaccatata gaagtatttt ataatcttat
     3961 gatttttata gaaatataga aaagtttatg tatcttaaaa tatcatttga tttaatatca
     4021 tatatagggt tgtttaacaa ttatctaagt aaaggcatac taccagtaag tttattcact
     4081 ttctttttat tcccataaat tgctatccca aaatactgta actcatcttc tggaacacta
     4141 gcaatctttt gtgtaaattc ttcataaaga ttgcaacttt gtgctacatc tgaaaaatct
     4201 acaacagtta aatcgttaaa ttccaaagta tataattttt tacgtaaatc ttttattatt
     4261 tctttgtttc cttttaaaat aggtacagga atgtcaataa ttcctaagtg gctattacct
     4321 gttttgtcaa ttacatctgg cccaacagtt tctggggcat gttttcctag tgtaattccc
     4381 attatagctg ctgtattgga tataattccc attggtaaat tttcgtctat taccatgaca
     4441 cattttgaat ttttattttc catttttatt tctccttttt accttattta aagttgtttg
     4501 aatcatcatt tataaatata ttttgatact gctttggtgt aagaccaata aaattcttaa
     4561 aaaagtttgt aaagtgactt tggtctacaa atcctgtttg tatcgcagca tctataggtt
     4621 ctacgccttt ttctaaaagc tttttagctt tgtttacccg tatattttct aggtatctat
     4681 atggtgtaat accttttaat ttcgtaaaat tacgaagtag agagtactta ttcatacctg
     4741 ctatagtgct taattcgtca agtactatat gttcagcata attattttct agataatcac
     4801 atactgcttg aatttctata tttgtatttt ccaagttaga ttgcaaattt ggctctgtat
     4861 gttcttcaat taattgctca attaggaaga aaaaaagctc ttccttttta aaatccaatt
     4921 cttcttccat gattatatag tgtaattctt gtagtagagg tactagctca ctgcgaaata
     4981 taacaggttg attaaatttt ggtaaataat ttttacctgt aatttcaaag acggtttttt
     5041 tcattatttc tggcttaata ttgatacatc tgtaatcaag tactttatca tctatttgtt
     5101 cacaagtatg actgtcaaaa gggttaaaaa gtagcaaatc tccagtactt gtggtatact
     5161 ctttattttt acaagataaa tatctttgac ctttttctat aaaaccaatt acataatgtt
     5221 catgaaaatg gtttgggaat ttttgcatta tgcctttaaa tctatatgct tcaattttta
     5281 attccctatc aaagcatact gtgcgttctt cttgtctcac ttaggtaacc tcctttcatg
     5341 ttatacaaag tgtaacacat atttttttat acttcttgta taatattgac taagagacaa
     5401 taatgtcaat tcatgtcaat ataatattac acttacagta ggtgtaatta ttatattttc
     5461 acaatgaata tgatataata ataaaagtta taaaataaaa agcaaatttt aattttatgc
     5521 ctagaagtta aaattattat aatgtaaaat aattttgatt ataagctatt taattgttat
     5581 aataatatta aaaatttaat ttaggagatg tacattaatg ttaagtatga atataggaga
     5641 aatagtagca agtttagttg gtatagctat ggcaatatca atacatgagt ttggccatgc
     5701 ttattcagct cacttattgg gagatgatac agctaaagct tatggtagaa tgacattaaa
     5761 tcctgtaaga catgttgatc caataggtct tttggctatg tttatcctta agatagggtg
     5821 ggcaaagcca gtaccagtaa atccaaataa ttttaaaaac tataagatag gaaacctgat
     5881 agtttcactt gcaggcgttt tttgtaatgt tttgactgct atactgtgtg taattatcaa
     5941 taagtatgtg catatgtatg ctataaatac aatagctgga tatgtgctta tatatagtat
     6001 gggatttgca gcttttaact tacttccgat acctccactt gatggatggg gagttatatc
     6061 tacatttgta ccatataaat ggaatgaata tttgtataaa tttgaaagta tgagttatat
     6121 aattttatta attgctttat ttactggtat atatcaaata atactatcac caatatatac
     6181 agtgttgcta aaaatagtat atttatttgt ttaatataat aaacagatta aagagggatt
     6241 gatatgaagt ataatataca attacaagtc tatgagggac cattagattt actttatgat
     6301 ttaataacaa agcataaaat agatataaag gatatatcta taatagatat aactaagcag
     6361 tatctaaatt atttgaaaat gctagataaa atggatttag agataactag tgaatttata
     6421 actatggcat caaaactttt agagataaag tctaaatatc tgctttataa gcaaaaagat
     6481 gaagaagaag acccaagaat agaacttatg gaaaagttag aagaatatag aaagtttaag
     6541 gtagcatcac aagatataaa agaaaatata acttatgtta atgagagatt ttatagaaat
     6601 aaagaagaga taattattga tgacaatgtg gatttagaag atatatcaat tgaagcaata
     6661 aaaaatatac ttccatacat atttaaagtt aaaacatcac agatagaaaa tgctaatgat
     6721 gagaaactag acaaaatagt aaggaagaag ataatatctg tggaagaaaa gatattgtat
     6781 ataagagata ttataaaaga taagattgaa gttactttta caaatattat aaaatcatat
     6841 gaaaatgatg aaataatagc tactttttta tctatattag agcttataaa agaaaaagaa
     6901 atagtagtag tacaagacat attttttgat gatattctaa taagaaaaag ttcggagtgt
     6961 taaaatgaaa agagaagata taaagtatat tatagaatct gttatgtttg catatgggga
     7021 accaataagt ataaaagaat taaactatat aataaataaa gaactttcat caaaagaaat
     7081 agagattatg ttaaatttac ttattgagga gtacagagaa caaaatagag gtattcaaat
     7141 aatcaagcta gagaataagt accaaatgtg tacaaataaa gactatgcag aatatataaa
     7201 gaagattatt gaaccaaaga aaaagaaaag tttgagccaa gctacattag aaactcttac
     7261 tataattgcc tacaaacaac caataacaaa ggtagaaata gaggatataa gaggagtaaa
     7321 atgtgataag gtattacaaa ctttgtttga aaatgaactt ataagagaag ctggtagatt
     7381 gaataagata gggaaaccaa taatttataa aacaacagat gaatttttaa agttgttgaa
     7441 tatagaaagt cttgaagaat tgccgcctat agaaaattat caagaagtgg caacaaatga
     7501 ataaaaatat aaaatcacaa taatttagta tgtaatttta aattttaaaa gatttaaaat
     7561 gctctaagtt gttgtgtttt tttgtataaa tataaaatta taattctctt aaaataaata
     7621 tggataatta agtat
//
LOCUS       DAEPOG010000171         7593 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEPOG010000171 DAEPOG010000000
VERSION     DAEPOG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.171"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(246..986)
                     /locus_tag="KR432_003091"
     CDS             complement(246..986)
                     /locus_tag="KR432_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0641606.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(1007..1795)
                     /locus_tag="KR432_003092"
     CDS             complement(1007..1795)
                     /locus_tag="KR432_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBH0641607.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(1883..1973)
                     /locus_tag="KR432_003093"
     tRNA            complement(1883..1973)
                     /locus_tag="KR432_003093"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Ser,seq:gct)
     gene            2232..2996
                     /locus_tag="KR432_003094"
     CDS             2232..2996
                     /locus_tag="KR432_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0641608.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(3277..4392)
                     /locus_tag="KR432_003095"
     CDS             complement(3277..4392)
                     /locus_tag="KR432_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0641609.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4433..5959)
                     /gene="glpK"
                     /locus_tag="KR432_003096"
     CDS             complement(4433..5959)
                     /gene="glpK"
                     /locus_tag="KR432_003096"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0641610.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            6252..7349
                     /locus_tag="KR432_003097"
     CDS             6252..7349
                     /locus_tag="KR432_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0641611.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         2509 a         1354 c          912 g         2818 t
ORIGIN      
        1 tcaacttttt tattatatct ttctcttgtt ttatctttat atttcgtatt tttattttta
       61 ttccccattt atttcccatc ctctataaaa tttttttaat tgagcttata tattatttaa
      121 atattatata tttaagatac catatattcc tacaaaaagg taacttacta tgtatctatt
      181 tttaattttc gcataaaaaa gccatctcct ttagaattaa ttctgtattg agacagctaa
      241 tttaactatt tatcctttga aacattttct acattatcat atttactatc aactaaaact
      301 atatcaactc ttctattttt tgacctacct tcttcagatg aattttctgc tattggtcta
      361 aactcaccat atcctacagc agataattta tctggtgcta tacctgcatt gtcaataaga
      421 agttgaacaa cattggttgc tctcataact gataaatccc aatttgactt aaactctgaa
      481 ttttttatag acatattgtc tgtatgacct tctactctca tgtaactatt catttcattt
      541 aacatcttac ctatttgaat aatcttatct ctagaattat cctttacaac tgcttttcct
      601 gtatcaaata gtattgtatc tttaaggctt attactaatc ctctatcttg aactcttaat
      661 gatactgaat tagccatacc attttcattt aaatacttct ggatttcttc accaactttt
      721 ttaaacttag cattttgtaa actatctaaa tcattacttc ctggttcaac tactggttct
      781 attttgctat taccaccttc aataactcca gaagaaccac caaaagcaga atttagagat
      841 tgagataatt gtttatattt ctccgcatca acattactca tagaatacat tattacaaag
      901 aaaatcataa gtaatgttat gaggtctgaa taagttaaaa gccatcgctc attgttttct
      961 tctttttcat cttcatcata caacataaac attccatcct tttatattat tcagctctac
     1021 caccaatttc ttcaagttca attagttctt ttgaatttaa aaatattttt aatttttctg
     1081 tcattaaatt agaattatca ccctcaagta tggataatat tgcttctatc accaaaagtc
     1141 tttcacttat ttcctgttga tttatagcct taagttttga tgctattggt aaccatatta
     1201 aattggcaga accaacacca tacaaagtag ccaaaaatgc aacagaaatt tgaggtccta
     1261 atgcatctgg attacttgaa agattaccta aaatatgtac taatcccata actgtaccta
     1321 ttatccccag tgtaggtgca tatccacctg ctgaatcaaa aattgctatt ccttctttat
     1381 gtcgttttga tgttatctgt atctgtgatt ccaatatatt tcttatagta actgcatcca
     1441 ccccatctac cataagttca agcccttttc taataaaagg gtctaattca tcattctcac
     1501 ttatttcttt gtcaacacta agaagcccct ctttcctagc ctttgttgca atttctttga
     1561 aatagattat attttctatg ttattaggct ttttattttt aaaagctaat aacattatct
     1621 ttatagactt tttaaacaca gggaatggag tcgaagctcc aacagcgcct atagttccac
     1681 caaacacaac aagtgctgat gaaaatacaa ataatactcc aatatgtccc ccatcaataa
     1741 caaaagcaac taccaaagat gctatcccca ctatgaaaaa aattaaagtt gtcatctatt
     1801 ccctcctttc tttaattata catatctaat catttatgac attataaaaa gactatcttt
     1861 aataaaaata gcctttaaca attggcggag aaggagggat tcgaaccctc gcaccggtta
     1921 cccagcctaa tcccttagca ggggatcctc ttgagccact tgagtacttc tccatatctc
     1981 tatactagta aattatataa taaaatcata ttttagtcaa ttgttttata aaatttgagt
     2041 tattttgaat tattaaacat ctatttttac ttttttgctt aaatgtaagt tttaaatatg
     2101 gtaaaattat cttgcataac acttataata ttatttgata aatatacata cattaacaaa
     2161 tatacctaaa ctctgactta attgtaagtt atgccaaaac taaaataata ctagtccaag
     2221 gaggatattt tatgttacca caggaaagat acgagaagat tttaagtatt ctcaaatcag
     2281 ataatataat taaaataaat gacatagttc aaatgtttga tatatcaata gaaactgcac
     2341 gaagagattt agcccatatg gaaagtatcg gtcttttaaa aagagtttat ggtggtgcta
     2401 taccagttat agcaagtagc gtagaactta actatgttaa aagaaaaaaa ataaactcaa
     2461 acgataaaaa acagattgct attaaatgta gtgagtttat aaatacaaat gatactattt
     2521 ttatggatac tggaactact gtacttgaag tttcaaaaaa tttgaaagat aaaaagaatc
     2581 taactgtaat aaccaactca attttagttg caaatgaact tatgaatact aatataaaag
     2641 tatatgtgct tggtggctat cttagagaaa ctgaaggttc tttttctggt ccacttacac
     2701 tctcagctat tgaacaattt aatgttgata aagctatttt aggtgctgga ggtatcactc
     2761 ttgaaaatgg aatctctgac tatcacttag aagaagcact ggttagaaaa aaaatgattg
     2821 aaaggtctag aaaatctata gtagttgtcg aacctcctaa gtttggtata aattcatttg
     2881 catcagtagc tcctatagaa cacatagata ctattattac ctctgctagt atagaccata
     2941 atataataat agagttttct caaaaaggaa tacctttagt catagctgat aattagcttg
     3001 tatgattgat ttttagttcc ttgatgtaat aaattaaaat taaggagcca aataattatt
     3061 tatttagctc cacttatgtc taaactatac tttatatttt aattatgtaa ctactttcag
     3121 ttagcttatc atttttagtt atttgattgc cattttattt aatttatcag ttttggttat
     3181 tcgattattt tttaattgtc tgtcttttta atttattttt agttatttaa actcctactt
     3241 taaaataact taaatttgac ttaagtattg tttattctac actgcatctt tcttcatttt
     3301 aatactttgt tcttcttcca aagttaaatc ttttgtcata agtatagcaa gtgcaattgc
     3361 caatactcct ccaaccaatt ttccaattac aacaggtgta atcatctctg gaactactcc
     3421 tgctgtaaat cctaaatggt cccccaaagc tgctgtagca caaaccagcc aagcagtatt
     3481 tactactttc ccctttggac tcatatcttt catcatttta tatactggaa tactattggc
     3541 taaagtaaaa actaatcctg ctgcactagt ggcatccata cctaattttc cacccactat
     3601 tgtaagtggt ttattaagaa ctttaactaa taaatgtaat attggaaaag tacctaataa
     3661 cactattgca atacttccaa ttacttgaat accttctctt ataggtgtca tgccttttat
     3721 aagtactata cctgttattt cttgaaatgc tgctgctccc aaacctatcg ttatcaatat
     3781 agatataaat tttccaaaaa ctaatgaccc atttatcata agtgtagaat taaattttaa
     3841 acctactgcc aatattactg ccaatattat cactggtatc atatttataa ttaccattga
     3901 taagttaaat cctgctatta atcctcctat aatacctcca aaaggtattg ttatcaagcc
     3961 tatcagtaac ccttttgcaa agaatggtct gtcttcatat tctataagac ctagtcctac
     4021 tggaattgaa aatactaatg tacatccaag catggatgac acgactaatc ctgcaagcat
     4081 tcctgcctct ttattgtgtg caagttccat agccaatgga taccctccca tatcattcgc
     4141 taaaatagtt gcaaacatag atgggtcagc tccacagaga ttgaatattg gtattattat
     4201 aggacctagt acatctgaaa ttactggagc taaacaaact ataccaacca ttcctaaagc
     4261 caatggaccc attgaattaa acccttcttc aaactgctct ccaagtccaa atttatttcc
     4321 aattattcta tctaacgctc ctaaaagcac tcccacagcc attatgtata atataatttt
     4381 atcaaagggc atttttaatt cctcctatac atgtaaaata ctctattgta acttaattag
     4441 cacacacctt tttggtatcg ttttcccaat tcatagctct ttttactgct tgattccatc
     4501 caagtaatat ttcttctctt ttttgcttag tcatatttgg aatatatttt ttgcttattg
     4561 taaattcact tctaatttca tcaatactgt tccaaacacc acaggaaagc cctgccatat
     4621 accctacacc aagtactgtc gtttcaggat tttctgcaat ttcaattggt atatctaata
     4681 tgtcagattg aaattgcatt gtaaagttat ttttagtaat tccaccatct actctcaaca
     4741 tttttatttc tttccctgaa gccaaattca tggaatcaac aacatccttg acttgaagtg
     4801 caacagactc aagagtagcc cttactatat gttctttttt agtacttcct gttatcccaa
     4861 ccattgtgcc tctagcatat tggtcccaat aaggtgctgc tatacctgca aatgctggta
     4921 caaaatatac ccctccagta cttgatatgg aatttgccat gtcatcagtt tctgaataat
     4981 tttttataac tccaattcca tctctaagcc attgtattgc agcacctgct atatacactc
     5041 caccatcata agcatatgta actttatctc ctatcttcca agcaactgtt gtcaaaatac
     5101 cattacctgc tatatttaac tcctcaccta tattcatata taagaaacat cctgtaccat
     5161 aagtattttt aaccattcct ggttcaaaac atccattgcc aaataaagct gctggttggt
     5221 ctaccattga agcagtaatt tttatagaaa ccccacaaaa tgcctcctta tctgtagttc
     5281 caaaatcacc actagtaggc attatatctg ctaaaatact tctaggcact cctataatgt
     5341 caagcaactc tctatcccaa tccatatctc ttatattaaa aagcattgtt cttgctgctg
     5401 tagatacatc agttaaaaaa gattttcctc ctgtcatttt ccatattaac caactatcca
     5461 atgttcctgc aagtacttct ccatttttaa ttttttcttt tacacctttt acattatcta
     5521 ttacccattt aatttttgta cctgagaaat atgggtctat aattaaccca gtcttatcta
     5581 ttatcttatt ttctaaacct ggtatattct ttaaattttc cacataatca taagttctcc
     5641 tacactgcca tacgatagca ttgtaaagtg gtttacctgt atttttatcc cataacatta
     5701 ctgtttcacc ttgattatct ataccaattc cttttatatc actttcattg attccacttt
     5761 caaaagcctg ttgtaataca ttattactca cagtttttgt attattccaa atttctatag
     5821 ggtcatgttc aacatatcca gattgtggat atatttgagt atgttccata taatcgctaa
     5881 aaacaaactc tgatttttcg tttatcaata ctgctcttgt tcctgttgtt ccttggtcaa
     5941 ttgctaatac atatttcact taaaatgtcc ccctttattt tcagaattaa taaattcttt
     6001 tttatttttt gaatatataa tctattcaag ttatttatat tttatatcat catgtggatt
     6061 tatgcaatat tcattacaat attttttgtt atttttaatt atggtgatta aaaaagtatt
     6121 tttattgaaa tattattgca atattcatta ttttattcta taatctagtt aaaataacac
     6181 acaatatttt aatatttaca catagtcagt cattcgaaaa tttaaattta actttttaag
     6241 gaggctttac aatgaaaaaa acatatctat tcagtcctgg accagtaatg gttacagata
     6301 aagtcagaaa ttcacttctt cactatgata tatgccatag aggaaacgag tttatggatt
     6361 tattcaaaga tacacaacaa aaaatcaata aactatataa tgcaacttct aattactact
     6421 cattaattgt atctggctct ggaacatctg taaatgaatg tgtactatct tctatctttg
     6481 acaaagaaga tgcagttctg ctagttagta atggtgtttt tggagaaagg ctagaagaaa
     6541 tattatttac ttataatgta aaaacatata agcctaattt tgaatgggca gaatacccag
     6601 acctggacat tttagaaaaa tatataattg aaaattctga tataaaagta attgctatgg
     6661 tgttccatga aactagttca ggaatgataa atccagtccc tgaactagga aaacttgcta
     6721 agaagtataa caaaatattt tttgtagatg caataagtgc ctcagcaggt gaatatattg
     6781 atgtagaaga gtttaatata gatataataa caggtgttgg tggtaaagct cttggagcat
     6841 ttccaggttc tgcatacctt tgtgctaaag agagtatttt acaaaatatt aaagaaaatc
     6901 aatgtaagaa tgtatatctt aatctttata aacactataa agttgctaaa tcatcttctc
     6961 aaacaccaaa tacacctaat gttactttaa tatttgccct taatgaagct ttaactgagt
     7021 ttttggaaga tgacagtaaa atagaaagat acaaagaatg ttctgctatt ttaaggaatg
     7081 gcatggaaga attggggcta acatttttac ttccagataa gtatatgtct aatacagtaa
     7141 catctgtatt tctacctaaa gaaatcaatg taaatgagtt tatattaggc ctagaaaggg
     7201 aaagtggcta tgttgtctat ccaggaaaag gcaaattcct agatgctaac atattccaag
     7261 ttgcaaatat gggagaaata tatccagaag attgttataa attcttagat atcttaaaat
     7321 caaaattgga gataaaaaaa gttatataac agtttgtttg taaaatccta caatacatca
     7381 gtataaatta aatagactga tttttttaaa ttttcctata ataaattata ttttatattt
     7441 ttgtataagg gtgaactata atatgtaaag ttcaccccta tttcaaatct ttttccttca
     7501 gtataatctt tttcttttaa tttattttat atttttgaca taattattct tacatttaat
     7561 tgttttttat ttttgacgca atcgtttttt att
//
LOCUS       DAEPOG010000172         7589 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEPOG010000172 DAEPOG010000000
VERSION     DAEPOG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.172"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..352)
                     /locus_tag="KR432_003098"
     CDS             complement(<1..352)
                     /locus_tag="KR432_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0641612.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGT"
     gene            645..862
                     /locus_tag="KR432_003099"
                     /pseudo
     CDS             645..862
                     /locus_tag="KR432_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..1282
                     /locus_tag="KR432_003100"
     CDS             920..1282
                     /locus_tag="KR432_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641613.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1266..1982
                     /locus_tag="KR432_003101"
     CDS             1266..1982
                     /locus_tag="KR432_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641614.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2002..2421
                     /locus_tag="KR432_003102"
     CDS             2002..2421
                     /locus_tag="KR432_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641615.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2411..2935
                     /locus_tag="KR432_003103"
     CDS             2411..2935
                     /locus_tag="KR432_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641616.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3247..3804
                     /gene="efp"
                     /locus_tag="KR432_003104"
     CDS             3247..3804
                     /gene="efp"
                     /locus_tag="KR432_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH0641617.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3896..4351
                     /locus_tag="KR432_003105"
     CDS             3896..4351
                     /locus_tag="KR432_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0641618.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4514..5224
                     /locus_tag="KR432_003106"
     CDS             4514..5224
                     /locus_tag="KR432_003106"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH0641619.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5278..6348
                     /locus_tag="KR432_003107"
     CDS             5278..6348
                     /locus_tag="KR432_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0641620.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6376..>7589
                     /locus_tag="KR432_003108"
     CDS             6376..>7589
                     /locus_tag="KR432_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0641621.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLST"
BASE COUNT         3222 a          643 c         1361 g         2363 t
ORIGIN      
        1 ctgttccgat ggggtcttgt agagatactt gagttttatt ttttttgtta gatctaatat
       61 tcattaaaat ctcattttca atacatctag aagcatatgt tgctaatcga gttcccttat
      121 ctatgttata agtttctata gctttaatta atcctattgt acctatggat attaaatcat
      181 cctgatcttc cgttgaatta ttatatttct ttgctatata agctactaat ctcatgtttc
      241 tttcaattaa tgtatctttt gcacttttat cgttttctat tttaaattta gttaaatact
      301 caatctcttc ttcaggggtt aagggttttt caaaagattt aagagctgcc aataggtcac
      361 ccccttttca acggtcactt taaattatat gaaggagact tatccaagtt gactgttatc
      421 taataaaata atatgcatat ttttagaatt taaaacatat atttgcaaac ttttgaatgg
      481 aatatgacat aaagagtggg aatttaaatt taagtttttg ttgtatatat atgaaacaag
      541 gcgaagaaat tattgattta taaagtataa attggaattg aggtgtcaat aaatataaaa
      601 ggaaatagta tttatatatg ttttggtgtt gaggtaaaag attaatgcaa agcattagag
      661 tctataaaaa tatgaatata ggaggtgtaa ttttacaata cttaaaaaag taaatgtaat
      721 taattgtttg tataaagttg attacaagca aaaagtatca gtcatagtta attctgttaa
      781 ggatgaaggt ggtgtgttaa tttatatatg tatgggaaga aattttgatt acaatgaaaa
      841 aacttacaaa agatatttat gaaaaaactt atagttaaat gtttatatat aaaatattat
      901 ttaggggttg tttttattat tgaaaataag aaagagtggt tttatatcaa tcgaatgtat
      961 aataagtatt gctatattat atgtggctgt ttatttagtt tctacatcat tgtataattg
     1021 ttatagtttt atcagtagaa atatatctga cagagaaatg ttaagtacag caaaaaaata
     1081 tatagaagat gagaagtata gaatacaaaa tagtaagtat gagttaattg aagataagat
     1141 agaaaaaaat tacataaatg gatatgaaat taacagtaga atagagcaaa ttttagatta
     1201 ttatcaatgc tatgaaataa atatagagat aaaaaatgaa tttaaaaaac tgaggtttaa
     1261 tagctatgtt actagaaaat aaaggctcta tattaataat aacattgata atattttcta
     1321 ttataagtac tgtgtgcatt atgtgtatag gtttaattta tacacataat aatatgtttg
     1381 agttaggata caaggacatt caactaaaag aaatttcttt aagtggaata gaaatatcag
     1441 taagtaatgt actttcttgt gttgaggaag ctattgatgt atctaataat gaagacgaat
     1501 ttagagaata tttcttagga aataatatga ttgaatgtat aaaaaaaata aaagatacat
     1561 cttatagtag tttagaagaa ctttctttaa aagtagaaaa taatactatt tatgacagag
     1621 gagatttttt tgaatttgat atagaaagta atgttagaga aaaagaattt ttaaaagcaa
     1681 taaaagtaag agtgaaaatc aaaaatccat ggataggaat tgacattgaa gattatatgg
     1741 ataaatctaa taacaataaa attgatgaca aaacattcaa taattataaa gcatctgaaa
     1801 ataatgaaat tctaaatcaa tttaaaaatg aaagtgaaaa tataaggaat caagaggttg
     1861 ttgaatttac agaagaagat gaagaatatg aagaagaact taaacaaaac ataattgaaa
     1921 atataaagaa gaattttgat gaaaaaaaac tagtacaggt atataagtat aggggtgtat
     1981 aactttgaaa aaagaaggaa tgtgactgta atagaattaa tagtatctat cagtataata
     2041 ctcttgatag ttacattatc ttttccaaaa gataatcttg aaaatcataa aataaattta
     2101 tttgcacgtc aactttgttc agatataagg tatgttaggc gaattaatat gtttagcaac
     2161 tacaatacat atatatatta tataaatcaa ggtaaacacg aaggttatgt attgagaaaa
     2221 gatggtaaaa acattaaatc aataatgcta cctaaaaatg caaaaatagc tagaagtatt
     2281 gaaataataa agtttaaatc tgatgggtcg cctcaaaagg cgtctacaat agagatatat
     2341 aataatagat taagaaaaac tataacaata acacctgtaa gtggaagagt acttctgaaa
     2401 gagggaaaat atgaaacata aatatggtta cttgcttttg gaaagtgtag tatcattgtc
     2461 ttcaatggtg ataattatac tagttttata ttctatattt ttgtctacaa taaacttaaa
     2521 gttaaaagta gaagataaga tagaactaca acaacaaagt cttgaaatca taaaatctat
     2581 ggaaggaatt attagcaatt ctatgggaat aatgaatgta tctaattatg aagaaacatt
     2641 taaaaagaca acgtctataa aatgtagata tgtagatgaa aataataatg aagaaagcat
     2701 aagtaataaa gaaattatat tgaatgaaag acgaaataag ttatttgtaa attctttaaa
     2761 tggcgaaagt agtcaagcag gtggttatga aattggagat tatgtagatg agatgtatgt
     2821 attaattact aataatggtc aatatgttaa tattaaattg aaattatcaa aacgtagtca
     2881 aaaatatgag actgatttca aaataaaagt atggaatttt agtgaaagta tataaaaata
     2941 cataaattta agataaagaa atgacatcaa taagtcatat aaaatataat tattgagaac
     3001 tatataacta aataatatca atatgtaaag tgtatagatt aagaatatag aggataataa
     3061 taaaaatata gaataagtaa tttgccaaat acttgtttat attgtataac ttatgatatt
     3121 atgatattag taaaaatttg taatcctgac ttgacagaat tcaagtaaga gatataattt
     3181 taaaggcata gtaatgtaaa atttactaaa aaataagaaa tttattttaa tatggaggaa
     3241 caaaaaatgg tatcagcagg tgattttaga aaaggtgtta catttgaaaa agatggacaa
     3301 ccatgtttag tagttgattt tcaacacgtt aagccaggta aaggagctgc tttcgttaga
     3361 actaaataca aaaacttaaa aacaggagct ataagagaag aaagtttcaa tccaagtgaa
     3421 aaattcccta aggctgttat agatacaaga caaatgcaat atctatacaa tgatggtgag
     3481 ttatattatt ttatggatca agaaaatttt gaacaaatac cattaaacta tgagcaagtt
     3541 gaagatgcaa ttaagttctt aaaagaaaat gaagttgcta caataagatt ttaccaagga
     3601 caaccattcc aagtggaagc gccaaacttt gcagagctag aagttacaga tacagagcca
     3661 ggtataaaag gtgatacagc aagtaatgta actaaagcag ctacagttga aactggagca
     3721 gttgttcaag ttccattatt tataaatact ggagataaag taaagataga tactagaaca
     3781 ggtgaatatt tatcaagagt ataaaaattt atgtatataa aagcttatat gtcttaataa
     3841 tataagatat atgagctttt agtttatata taaaaaagaa agcgaggggt gttacatgaa
     3901 acatttatat gaagaagttg catacttaaa aggcctagca gaaggactag aaataagtgc
     3961 tgaaagtaaa gaagggaaaa tgatacataa aatagttgat gcattagaag tatttgcaga
     4021 tgctattgta acattggatg aagaacaaga agaacttcaa gattttgttg aatctataga
     4081 tgaagattta gcagatttag aagaagatat tgctgacatg gaagaagatc tttatgaaga
     4141 agatgacgat gaggatgatg aggacttcag ctatatagaa atggaatgcc caaattgtgg
     4201 agagttagta gaaatagacg aagatttgtt atatgatgat gaagtagatg ttgtttgtcc
     4261 tgattgtaag gctgtgatat tatcatcaga agatgattgt gatgatgatt gtacatgtgg
     4321 tggttgtcca agttgtgatg atagagagta aaaattcata agaattaaaa ctgcctatga
     4381 tgcctatgag atattttgct tcataggtag ttttattttt atgaggaagg gtattatgta
     4441 ttttggtttt gcaatacttg gacaaaaata gtacactaga atttatagaa taggattttg
     4501 gagggatgta aatatgaaaa taagtaaaaa attactagat aatatacaaa gatttgaaaa
     4561 tgttataaat tataaattta aaaataaaga atatatacta gaagctctta ctcatagttc
     4621 atattctaat gaaaataaaa aatataattt taacgaaagg ttggaatttt taggggattc
     4681 ggttttaggc atagtgataa gtgattattt atttaatgaa gaagctaatc tgcccgaagg
     4741 ggaactaact aaacttagag caaatatagt ttgtgaagat tcgttaagtg aagtagctaa
     4801 tgatataaat ttaggtattc atatgttatt aggtagagga gaagaagcaa caggaggaag
     4861 acataggact tcaatattag cagacgcatt tgaagctgta atagctgcta tatatcttga
     4921 tggaggtttt gaaagtgcaa gacagtttat acttcatcat atggaaaata taatttatga
     4981 ttcaagaaaa ggaaatatat ttagagacta caaaacgcat cttcaagaag tgttacaagg
     5041 gaatggagaa aataatatct ggtacaggct tatagaagaa aaaggaccag accataataa
     5101 aagatttgta atggaagttg gaattaatga cgatgtattg ggtataggag aaggaaagag
     5161 taaaaaagaa gctgaacagt tagctgctaa aatagcttta aagaaaaaat tatgggaaaa
     5221 ataaatataa tatgtaaaaa tataaaaaga aatatttata ttagaaggat gttgtagatg
     5281 aaaaagagaa ttatacctat atttgttcct cacaaaggat gtcctcatga ttgcatattt
     5341 tgtaatcaaa aaaaaataac aggtgtgagt actgatgtta ctagtgaaga tgcaagaaat
     5401 ataattgagg aatgtttaga gactatagat aaagatgcag atgtagaaat agcatttttt
     5461 ggtggtagct ttactgctat agatattgat atacaaaaaa gtttattatc agttgctaaa
     5521 gaatatgtag aaaaaggtct tgtaaaagat attagaatgt ctacaagacc agattgtatt
     5581 gatgaagata tattaagtat gctaaaagaa tataagacaa gtataattga actaggagta
     5641 caatccttag atgaaaaagt attattagat agtataagag gtcatcaatc tgaaattgta
     5701 tacaagagtt caaagatgat aaaaaatagt ggaatcaaac ttggattaca gatgatgatt
     5761 ggtcttccag ctgacacaga agaaaagtgt atatttacag caaaaaaatt tgtagagcta
     5821 aaaccagatt gtgttagagt ctatccaact ttggtagtaa aagatacagg tcttgaaaag
     5881 ttaatggaac aaaataaata taatccattt actcttgaag agagtgtaaa tatagtaaaa
     5941 aaagttcttg tactgtttta tgttaataat atcaatgtaa taagagttgg gcttcaagct
     6001 actgatgata tacaaattgg aaaagctgtt ttagcaggac catatcatcc tgcatttaga
     6061 gagttggtag aagcagatat gataaaagat tatttagaat ttgttatttt gcaaaataaa
     6121 aatataaagc aaatgttggt caaatctaat aaaaagaata tttctaaaat cataggaaat
     6181 aaaaaaacaa atgttaaata tatggaagaa aaatttggtg tgcttttaaa aacacaagaa
     6241 tccgatttag atataaatca attagaaatt gtgttagatg ggaaaagttt aattagtgct
     6301 aatatgagag acattcatag aaaattgtat gacatttatg acctttagtt ttaataatga
     6361 agggagagaa aagatttgta tttaaaaaga ctagagttaa agggatttaa atcctttcca
     6421 gtaaaaacag atattatttt taaagaagga ataacagcca tagtaggacc aaatgggagt
     6481 ggaaaaagta atatatctga tgctgtaaga tgggtattag gagaacagag tattaagagt
     6541 cttagaggtg ataaactcga agatgtaata tttgcaggta cagatacaaa aaaacctatg
     6601 aattattgtg aagtggctct tactattgat aattctgaaa atcaattgga attagatttt
     6661 acagaagtaa ctataagaag aagggcttat agaaatggtg aaagtgaatt ttttctaaat
     6721 aataaaagtt gtagattaaa agatataaaa gaagtctttt tagatactgg tattggaaaa
     6781 gatggttatt caatcataga acaaggaaaa gtagatgaaa tattaagtaa taatccttta
     6841 agcagaagaa aagtttttga tgaagcttgt ggcatatcaa aatatagata taaaaaacag
     6901 gaagcagaaa ggaatttaag taatacaaag gaaaatttag aaagaataga tgatgtatat
     6961 atagaaattg aaaatcaatt aaagcctctt tttaatcaac agacaaaggc aaaaaagtac
     7021 ttggaaataa gtgaaaaatt aaagatactt gaagtaaata gctttataag agaaattgaa
     7081 ggaatagaaa aagaattaag tgaggttaat gaacatcgta aggttattga aaaggagctc
     7141 aatgaaaaag aagaacaaaa aaatgttgta gagaaaaagc aagaagatat taataaagag
     7201 gtagaggtat tacaagatgt aattgaaaaa tctgtagatt atataaattc aattaaagga
     7261 gttatatcaa aaaaagagtc ccaaataaat ttaattaagg aaagaataag aaattttacg
     7321 aatgaaatta gtagaaaaaa tttagaaata aatgatataa aagagaagct aaatgaaaat
     7381 aagcaataca taaaagaatt agaaagtaat aaattatcag gtagtgaaga actatctacc
     7441 ttgcaggaaa atataaaagt attggaggca agtaaagata aacaaaaaat taagttagaa
     7501 tccctaaata atgagataga attattgaaa gaaagtataa ttgatatatt aaataagaaa
     7561 caagagttta gtaataagct gtccacttt
//
LOCUS       DAEPOG010000173         7560 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEPOG010000173 DAEPOG010000000
VERSION     DAEPOG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.173"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            199..828
                     /locus_tag="KR432_003109"
     CDS             199..828
                     /locus_tag="KR432_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641622.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            963..1289
                     /locus_tag="KR432_003110"
     CDS             963..1289
                     /locus_tag="KR432_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641623.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            1305..2594
                     /locus_tag="KR432_003111"
     CDS             1305..2594
                     /locus_tag="KR432_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0641624.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            2618..2947
                     /locus_tag="KR432_003112"
     CDS             2618..2947
                     /locus_tag="KR432_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0641625.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            3095..4423
                     /locus_tag="KR432_003113"
     CDS             3095..4423
                     /locus_tag="KR432_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0641626.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            4740..5600
                     /locus_tag="KR432_003114"
     CDS             4740..5600
                     /locus_tag="KR432_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBH0641627.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            5805..6785
                     /locus_tag="KR432_003115"
     CDS             5805..6785
                     /locus_tag="KR432_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0641628.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            6988..7437
                     /locus_tag="KR432_003116"
     CDS             6988..7437
                     /locus_tag="KR432_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641629.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
BASE COUNT         3030 a          756 c         1348 g         2426 t
ORIGIN      
        1 ggtaattttt tttagtctaa aataatatta atattagaca catataaata tacttagaca
       61 aagtatctaa gttttgatga taaaaatgat tgaaataaca gtgttttaaa gtgtctaaaa
      121 aaactggcat aatacttgct tatattatta aatgagtaat ataaataatt aaaatttaaa
      181 taaaaaggag taaataatat gcaagataaa atattaagag ataaaaaggc aaatgaccca
      241 tgggcaagaa tgactacaag aaacggattt agtgcagacg aaataatatc gtcatttcaa
      301 aaagctataa gaagaaatat ggtggaagaa gcttgtgaat ttgcttatga actatatata
      361 tcatcaccag aattagaaga taagctatgg agaaggatac ttacaatatc agttgaagat
      421 ataggaatgg gggacccatc agctgtaata atcataaata atttaaatca agtaagaaag
      481 gaatattcat atgcagatgg agacagacct ttatttttca tacatgcaat aagatatctt
      541 tgtgcatgtg agaaagatag atcaagtgat ttattaaaaa atataattat aaaaggattt
      601 gcaatgggta aagttccaga agttatggac gtggcacttg acaagcatac atatagagga
      661 ttagaaatgg gaagggattc attccacttt ttaaatgaag ctagtatagt aataccagaa
      721 aaagaagtag ataatgatta taaagaaaga tacttaaaaa tattagaaaa atataagcaa
      781 gaagaagtta tagatacggc tttcaagttt aatcattggc agtactaaga attagagtaa
      841 atattaataa gaattaaaat aaaataataa gaattaaagt aaaaactgat aggaattaga
      901 gtaagaattg gctgataaaa aattagaaaa taaaagaggt tagatattgt aggagggtaa
      961 ttatgaatat tttattagta tgtgcaaatg gagcttcaac aggtgtactt gtggaaaaga
     1021 tgaaaagttt ttgtagtgag catgaaaagt taaaaactaa aactataaat atagaggcaa
     1081 catcattcga aaatttaaaa tcatacattg aagctaatga tacagatgta gtacttgtag
     1141 caccacaaat taggttcaag gaagatgatg tagttgaagc ttgtaagaat tacaaaatag
     1201 cagttggttt aatagatact aagcattatg gtcgtatgga tgctccttct gttatgaagt
     1261 cggcaatcga attatataaa aatagataat tggggggaat aaaaatggaa gcacttcaaa
     1321 ataatttaag aagatttctt ctaccaatag cacaaaagat tgaaaaacaa aggcatcttc
     1381 aagctataaa agaaggtatg ataagcatta ccccaataat aattgttggt tctttaagta
     1441 tgttatttat ggcattaaac aatatgttac ctgaaggaag tgcaaaaaca ttattatctg
     1501 aaaatatgga tacattactt atccctaata aatttactat gagtttatta tctatatatt
     1561 cagcattttt tatagctcag gcactagcta aaaaatataa tcttaatcat gtagaaatag
     1621 gtatgacagc agttgttgca caacttgttg tatgtggaca agtagtagat ggtgtattag
     1681 atacaagtta tttagatgca caaggtttat ttgtaagtat attagtagca cttttagtag
     1741 ttgatattac taagtttatg aatgataaaa atctcgtaat tagatttcca aaagaagtac
     1801 ctagtgtagt aaataaaagt tttagaaatt taactccaat gatagtatgt ataatgttat
     1861 ttacagcaat agcagcaata actaaaaatg tctcaggtca accattacca gctattataa
     1921 tgaatttctt agcaccagct ataagcagtg tagataatgt ttttgcagta actataatac
     1981 tatttataac tcaattatta tggttctttg gccttcatgg agcagcaata acttcaagta
     2041 tatggatgcc aatagcagct acatatatgg cagaaaatgc aacactgata gcagcaggag
     2101 gagacccaaa atatgtgttt acaataggat tttactatgg tttcttgcaa gtaactggtt
     2161 cagggataac tttgggatta gtgtacctta tgtcaagaag taagtgtaag agttttaata
     2221 gtatgggaaa agttgtgatt ttaccatcac tatttggaat aaatgagcca gtaatatttg
     2281 gaacaccaat agttatgaac ccatatatgt ttgtaccatt tgtatttgga cctgtattgg
     2341 ttggagcact taattttatg gcattaaaag taggattagt aggattacct attgcagaac
     2401 caccaggctt tttacctcca ggggttgcag catttttgat gacattagat tggaaggcaa
     2461 tcgtattagt atttgcaagt ataatactta tgaccttgat atattatcca ttctttaaga
     2521 taatggagaa ggaagaatta aataaaaatg cagaattagc tacaacatta gatgatgata
     2581 gctttgattt ttaacaagtc attagaagga gaaggatatg gtacctgaaa tggaagaaaa
     2641 agtatttagt ataatatcag aagcaggaga tgctaaaagt gatgtaatga tgtctcttaa
     2701 ggaaataaaa aaaggtgact acaataaagc aaaacacctt ttaaattcag cctcagagaa
     2761 aatacaaaca gcatcaaagc atcatttaga attactatca aatgctatga atagtgaaga
     2821 tagtgggact gattttttag ttgtgcactc tgaagaccat tattcaaatg cattatttgc
     2881 tcattcttta gtatctgaac ttgtagatat atttgagatg atggacccaa gaattaataa
     2941 gaattaaatt tcttgtaaat agtgcttgaa gtttggctat agtttattaa atcaatagat
     3001 ttaataaatg aactcccttt tactttaaag tactagtatt tataaattta tttataaata
     3061 cagtaaattt ttaaatagaa aaggagatat tattatggaa aaattttcag tagtaatagc
     3121 tggaggagga agtacatata ccccagaaat catattaatg ttgctagata acttagatag
     3181 gttaccatta agagcaatca aattatatga taatgatgaa gaaagacaaa ataaggtagc
     3241 taaagcttgt gaaatactaa taaaggaaaa ggaccctaat atagagtatt tagcaacaac
     3301 atgccctaaa gaggcttata cagatgtaga tttttgttta gctcatataa gagttggaaa
     3361 acttgaaatg agagaactag atgaaaagat acctctaaaa catggagtag ttggtcaaga
     3421 aacttgtgga ccaggtggaa tagcttatgg aatgaggtct atagaaggtg ttcttgaaaa
     3481 catagattat atggaaaagt attctccaaa ttgttggatg ttaaattatt ctaatccagc
     3541 atcaataata gcggaagctg ttagaaggtt gagacctaat tctagggtaa taaatatttg
     3601 tgatatgcca ataggtatgg aacataatat agcaaggatt gcaggactca aatctcgtaa
     3661 acatatggat ataagatact ttggattaaa tcactttggt tggtatactt caattaaaga
     3721 taaaaaagga aatgaacttt taccaaaatt agtagaacat ataaaagaat atggttttat
     3781 aaatggtgaa gaaggaatga aaaatgacaa gaaagatagt tggtttgaaa ctaatttatt
     3841 tactaaagaa atagtaaaaa cagatttaac tacaatacca agtagttact taaagtacta
     3901 tctatttcca gattatgttg taaatcattc tgatgtaaat tatactcgtg ccaatgaagt
     3961 tattgatgga agagaaaaag aagtttttgg ttcatgtgca ttgatagaaa agcaaggaca
     4021 ttctaagggc agtgggctta agataggaat acatgcagag tttatagtgg atttagctac
     4081 agctctagct tttaatacac aggagcgtat gttattgata gttcaaaata atggagctat
     4141 agaaaatata caagatgatg ctatggttga aattccatgt atagttggaa aagacgggta
     4201 tgaaccatta tcaataggga aaataccaac attccaaaaa ggtttaatag aacaacaatt
     4261 agcagttgaa aaattagtag ttgatgcttg gattgagaag tcttataata aattgtggca
     4321 agcacttatg ctatctaaga cagttcctag tgcaacagtt gcaaaagaat tactagatga
     4381 tttaatagaa gcaaataaaa attattggcc aaaacttaat taagaataat atatttttaa
     4441 agttcctaca gtttagtaca tagcctagtt aatgtatttg tagggacttt atttatttac
     4501 aaatgagata caagtggcaa acatagtgta ttttgcagtt aggaaagtag gtttagttac
     4561 ttgttagttg gattaaattt aggtacaata atttactatc aatcaataga aataaccaga
     4621 gaagttgcat aataaaaaat ttacactttt aacaggattt atgtaggtgt ttatagaaat
     4681 aaataaatat gtacataaat acataattat ttttaagtct aaaatacaag aatggagaga
     4741 tggctatgaa gtggagagat tatgctgatg atgtaaattt atttgaggat tttgataaaa
     4801 ctatcaagcc tctaactgac gaacaaagaa gaaaaaatat aaacacttta attgcatatt
     4861 tttcaaaaga agttccttct aaaatataca atttatcaaa tgatgaaatt aagcctagag
     4921 atatacttag aggacttttg aatgtatatc caccaaaaga aattgctcca gagatactta
     4981 atatgttaca taatttattg ttgattgaat gtgaagaaag agagttagta gatgtaaatg
     5041 atatagaaga agtagaggaa gggattgcca tatggagagg taatataacg aatttaagag
     5101 cagatgcaat agtaaatgct gcaaataaca agttattagg gtgtttacaa ccattacatc
     5161 tatgcgtgga taatgagata cattcatgcg caggtcctag acttagagaa gattgtgaca
     5221 aaataataaa aaaacaaggg catttagaat atactggaga tgctaagata actagaggct
     5281 attgtttacc tgcaaaattt gtagtacata ctgttggacc tattgtatct ggtggtcagc
     5341 caagtaaaga gcaagaaaag caactgcttc attgttataa atcatgttta aatacaataa
     5401 aagaaattga tgaaataaaa aatattgttt tttgtggaat ttctacaggt gtatttggtt
     5461 atccaaaaaa agaggcagct aatcttgctg taagtagagt tagactatgg ctaaaagaaa
     5521 atcctgaaaa aaatttaaaa gttgtattta atgtgtttac tgaagaagaa gaagagaaat
     5581 atagaagaat atttaaataa tattatgtaa cagaatccta aaaatactta ttatataata
     5641 caaattcatt gtataagata ataatttaca agtagtaaaa attaataaaa tataatttgt
     5701 atttttaata aataagtagg tataatagaa attataagaa tagtataatc aattatgcta
     5761 ttacttagaa aatataatat aggaaaattg tatggtaaat aaatatgaaa aatagtttta
     5821 aaaattgtat tagtttaaga aataaattta taattatatc aattgtgttt ctattaatcc
     5881 tattaattgt atcttatata tttggcaaat tagtgtataa tagttcggtt ggtttagagc
     5941 agttaataaa aaacgaagat atagtaaaaa tattttctga gagaaaagat aaaccactgg
     6001 agaaattaaa aaactacaag acatctgaat taatgataca aagttctaat aattacaagc
     6061 ttgaaagtct ttttataact tcaaatataa agacaagaga tactatgata cttgttcatg
     6121 gaattggaag tagttactat gaaatgttaa aggtagcata tagatattta gataaaggtt
     6181 ataatgtatt agtttataat caaagaaata ctggtaatag tggtggagat aattacacat
     6241 ttggtcttta tgaaaggtat gatttagata gtctagttaa atttgtaaaa aataaatttc
     6301 ctgaaggaag attaggagtg catggatttt ccatgggagc agggacagct gctatgcatt
     6361 cagagataaa ttcaaaagat gataaggtcg atttttatat actggattct ccttattctg
     6421 aaatgaaaga tgcaattaga atgggagtac tagaaaaacg aataccagat atattaatca
     6481 attatgtggt gatttgtgga gatttatata ataagtttaa atctggattt tggtacagtg
     6541 atgtaaaacc atatgagtca gttgagaaat ctaatgtacc catactgttt atacatggaa
     6601 caaaagatac tgtttgtaat tatcaaaata gcaagaaaat gtatgattta gttaaacatg
     6661 ataaaaaaga cctatggctt atagaaggta ttggtcatgt tgatggattt gaacatgata
     6721 gtacagtata cttcaataaa atatttaaat ttatagatag taatgtttta agtgataaaa
     6781 gttaattgaa tattagtagc ttatttattt ttgtatttat taaaaaatag ataggctatt
     6841 tttatgttta aatataagtt ttgtttagta atgtgaatta atttctagta aaaatgaaaa
     6901 taactaccaa ttaaattcat ttatacctca aataaaagag ttagtattag attacaatca
     6961 aatctaaaga aaagagaggt agtatatatg aaaaacaaaa ataataaaga taaaaacatg
     7021 ttgaatatta aagtattgag agttctagga gtgctattaa tactttttga ttatacgggt
     7081 gatactttga atttttataa tttcttcgca aagccattag tatcgggtaa tttatataag
     7141 acaacaggta ctgactgtat tgtattttta ttatttattg tagagtgtgt atacctagtg
     7201 ggaagatata tattaaaagt taagataaat tggagtgaga ttattgaaaa tgagaaaggt
     7261 aattttataa ataacttgtt ggttgaaaaa atacaagaag aaagcaacat tgtatataat
     7321 ttattaaact catatgtgtt gccacatata tataagaaag caagggtgtt gatgtttaaa
     7381 acatatcata tgttaatcta cattaaagaa aaatactatt atgtaaatat atattaatat
     7441 aatatgattg cacaattaat tatattatat tttgtattgg atgataaaat gaaagacata
     7501 aattttttga gacactaaaa tcgcaattta tttcgtgttt aagttataaa ttttgtgaaa
//
LOCUS       DAEPOG010000174         7549 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEPOG010000174 DAEPOG010000000
VERSION     DAEPOG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.174"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(42..3377)
                     /locus_tag="KR432_003117"
     CDS             complement(42..3377)
                     /locus_tag="KR432_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0641630.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3427..3855)
                     /locus_tag="KR432_003118"
     CDS             complement(3427..3855)
                     /locus_tag="KR432_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641631.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3845..4225)
                     /locus_tag="KR432_003119"
     CDS             complement(3845..4225)
                     /locus_tag="KR432_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBH0641632.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4258..4566)
                     /locus_tag="KR432_003120"
     CDS             complement(4258..4566)
                     /locus_tag="KR432_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641633.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4599..5783)
                     /locus_tag="KR432_003121"
     CDS             complement(4599..5783)
                     /locus_tag="KR432_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH0641634.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(5787..7250)
                     /locus_tag="KR432_003122"
     CDS             complement(5787..7250)
                     /locus_tag="KR432_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0641635.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
BASE COUNT         2320 a         1442 c          726 g         3061 t
ORIGIN      
        1 tctctacata attaacaata attttgcact aaataacatc attagcctat ctctgatgca
       61 ttttctttaa ttttatttat agttttaatc aatttatttc tctcttcaat atgattttca
      121 aataaatcaa ttatacttcc aacagttcta aatgggtctt ccatcaacac tttgttatcc
      181 tctataaatc cattcttgac tacataatcg actataagtt taacaaaatg tatttgctta
      241 gtattaaaac tcttattatt taaaaactct gaaaaagctt catttgcagc ttctctatcc
      301 aatcccacta gttttctcac taattgactt actggagtat ctccaaactc ctttttataa
      361 tcatcacaat tacctaactg ctttaagagt atttcctcca acgtttcaac atcttgcttt
      421 gttatctttt tattatttct taatttaaat attgctaatt catctttatg gctatttaaa
      481 taatactcta ccttcttttt ataattctta agattgtttg cattatatac agaatcattt
      541 ctatcttctg ctacaatcat atcttcaaaa cttgtataat atatcttttg actagacttt
      601 tcaagatact taataagctc tcttaatgat atacgtactt cttctacctc aaatatatct
      661 gaatcttccc aaaaatcctc tgtcattgct ttttcaatag tatatttttg ttgctggatc
      721 tgtggtatag tcccaagttt cgaaagtttt tctgctgtaa ccattacatg ccttatccct
      781 ttacttgcat tattattttg taagtatgca agttgtatat tatacataac tacatcaaat
      841 cttttagcca attcatcatc actatatgaa gttatgagtg gtgatatata ttctcttatc
      901 tcattttgtg ctactgctcc aatattgctc caaacatttt catttttata cttatgtaca
      961 taacttaaat tcatcctaac tatataacta tcctcattta aagcttttac agaagttata
     1021 agttcttcta acaattcttt tctaagttct gcatacttac cttctatata tttaatatct
     1081 tgaagttctt taaccatact tacctttata ttaaataatt tttcagtaag tgtttctgct
     1141 ttatttccct caaatccttt aggattaaat ttaaaaaatt caaaattatt acaaaaatca
     1201 aatattaaga atctttcctt atccatatca acacctaata aatctggaca taatctagtt
     1261 cctctaccta tcatttgcca aaacttagtc ttacttctca ctttcttgaa aaaaacaaga
     1321 tttaggattt caggtatgtc aattcctgta tcaagcatgt ctactgaaac tgctatttga
     1381 ggtaatttat tcttatcaga aaaatcatct attatagaat ctacataatt tatactatag
     1441 tcaactgctt ttacaaagtt cccaccatac tttggataca gtttattaaa tctttcaact
     1501 attgccctag catgctttgt attctttgca aaaattatag tcttaccaat cttttcttct
     1561 ccttctattt tcaacccctc tgtcattagc ttatttaaaa ctaaatctat tgtgtcagaa
     1621 ttaaatagcc actcatttac tgcattattc cctatttcgt ctccaatact ctcatcatca
     1681 ttaaatgtat tttcatattc ttccttctct tcatcagaaa gctcatcata cttaattcca
     1741 tcttccataa tcttagtctt aaactcaata ctagtataat ctactaaata tccatcctcg
     1801 actgctttat tatactcata agcatatgta ggtactccat tttccatgtc aaaaactgaa
     1861 taagtatttt tatctatctc atcctttggt gtagcagtaa gacccattaa gtaagaatca
     1921 aaataatcaa agattgactt atacttttta taaatacttc tatgcgattc atctattata
     1981 attaaatcaa aatgaccagg tgtaaacaat ctctttccat ccttagactt cgtatcatca
     2041 attgcattca tcattgttgg atatgtagaa aatatcatcc tagcatcttc tgggctgtct
     2101 ttgctatcca ataaattaca taacgccaag ttaggtaata gcttagtaaa acttttttta
     2161 gcttgcttta ccaatgcttt tctatctgct agaaataata tatttttaat ccaattatgt
     2221 cttgaaagta catcaactat agatatagca attcttgttt ttcctgtacc agtagcacat
     2281 accaaaagag ttttgcgttg cttattttca agagcctcac atacagccct aatagcttct
     2341 ttttgataat atctattgga tatttcatca tttattgtta cagaacttaa agacttctta
     2401 ctctttcttc tatctatcat aagttgtaat tctgattttt tataaaatcc ataaactttt
     2461 ctatctgaat agtcatccca aatatacata tcaaaaccat tcgtcaaaaa tattactggt
     2521 ctttgactat atctcttttc caaacaatct gcatataatt tagcttgttg ttgaccaatt
     2581 ttcgggtctt tgctcgttct tttcgcctca actactgcta atggcttacc attttcacca
     2641 taaagaacat aatctacata tcctacacca ctttcattag gcataccttg tacttctatt
     2701 tcctccccta tatcttttcc aaattcccaa cctgcaagct ttaattctat atttatatat
     2761 ctttttcttg tctcaaattc gtttacttcg tctatcttga aatcgtagtt tacagtatta
     2821 acttttctct tttcagttag tgactttctt aatttctcat tttcttttat tacttcttca
     2881 agctttttat cttttgaact taatctctca taaagatttt taagctcgtc tggtctcgtc
     2941 cttttctctt ccccatgaag taataaatct tcattaaagt cttctgctgt atattcatct
     3001 gaataacaat agtctatcca actaacaaat tgatgcaaat tgttaagtga aagtattgct
     3061 tctcctctct ctatgttcgc actagtatgt acagataaat tacctaattt aactatatat
     3121 tttaataaag gtaacatatc atagtctata agttctataa aactattatt atgaatcaac
     3181 gtactaagat tatcttgata aggcaatact aaatcactat cattagcata tagccatttt
     3241 actgcaagct ctaaagctct tcttgttata gttgcacaag tagatgggct aactagtata
     3301 cttttttctg cttctaagca agcagtatta aaactattga aatttttctt cttttgtaaa
     3361 aagtcaaaat tacccaaggc ttttctcccc cttttgtccc ttagttaaaa taaatctctc
     3421 taaaaactat ttcttacagc tgtttcttat actaacaaat acttcttcat tttctgtaaa
     3481 caattcgtac atatcttcaa actgtggtat atcatatcca tgagaatcat catttctcaa
     3541 ttttctaagt gtttctataa ctatagaaga atttttaggt aataaattgt gatactcaca
     3601 agctaaaagg tagtcataaa agtttttaga ttttatgttt aaatcaattg atttataata
     3661 caacattgct tttgttaatt ctaaaaatga gaccatatag cttttaaaac cttcttttat
     3721 gtatctagcc cataactcag gcatttcttc cttatcatat aagattttga agttttgcag
     3781 gtcatataag caagaaccaa aatccctatt tacttcttct attgagttcc aagataattt
     3841 gtttctaatt accatacatg tccctccaat ctaagatatc tattacatca ctgttttcaa
     3901 aatagaattt atttatataa tccttaaaat ctttagattc ataaataact tcaccttcta
     3961 aaatagcttt tttaagtgct attgtataat tatctgtaac aatttttagg tctacatcaa
     4021 tgtctaatat atttcttaga gcttctgtct tagttgcaag ttctgtaatt ggtacttttt
     4081 tgataggaat ccaagctatg tctatatcgc tttctgatgt atgtttatct gttccataac
     4141 taccaaatat tatgattgct agagtatttt cttttggaaa tacatttatt attgagtttt
     4201 taatctcatt tggtatattt ctcatttaat cacctcttgt ttattaggta atattgctta
     4261 attgaattta tatccataat cttttaatct atttatacat atttctcttt cttcatcagt
     4321 aaacaactct ttatactttt cttttaatac taatacttct aatgataaat ctaatctctt
     4381 acactcccat agccttgtaa atccttcagt agctccttct ttattgatta attctttagc
     4441 agctaataag gctcctttgt tatttatcag ttgtaaaaat cttgttggct tatagttaca
     4501 ttgtaaatca gcttctttat agatatttac catgtcatga tataaatcta gctctatttg
     4561 attcatagat tttattcccc cattttaata cttgtatttc agttgaacaa ttctccttta
     4621 aaagcttttt gcattaatga gttaaagtta tcttctagtt cttttaagct agtttccatt
     4681 tcaaacttca aagagtttac ttgtttaacg aagttggcaa attgattttg aagtctaata
     4741 ggaggtacaa taatatttat ttttctaaca gcatctttag ttatcgtttt tgttacagct
     4801 cctcttactt tctcttttat tttactttcc atataggatg ttagtaaaag gttatataag
     4861 aatatatgat tcactttatc ttcattaaat ctaagcataa attgattcat agctaaagat
     4921 actggtttat ttaaaaatgg cattaaataa actttacctg cactacctat cttatttatt
     4981 ataacctcac caccaaacac ctttgatttt tcaagataat tatatgcatg ttcatcgata
     5041 tacttaacac ctttttcaaa gttattattt tctaaatctg tagttcttac cattaaggca
     5101 taaccttttg tatctaaaag agttacatta tctcttaagg tttcataact accatttgaa
     5161 tgataatcag ttaaaacatc taaatatttc cctatttctg aaatttccca gtttttttcg
     5221 ttctttgatg gggtaccaaa catctcaata aatcttgatt taactaattc atccaatact
     5281 tctatttgct cttttctttt atctattaac tcttgagcct tgtctaaaac acgcactacc
     5341 ttttcttgca cttttaaatt atacatgcgt atttttatat tagctaatcc ttcattattt
     5401 aaagctgatt gtgtagcacc tttacttaac ctattttttt gatttagaaa ataactacta
     5461 tttaagtaat ggtataaata ttgaggcaat atttcttttg atggttttaa aacataaaag
     5521 ccagtcgata ctattatatt tttattagtt ttattaataa ctagtgtttt ctttgtgtcc
     5581 ttcatttttg caaatataac ttctcctata tttacagttt gatttgctct agaaggttta
     5641 ttttcataag taactatttc tagattgctt atcttgttaa aatctaagtt tccagtacta
     5701 agatatgagc gttctccatc aaactttttt acattttccc tgcttatact aacaacatct
     5761 aaaatttttg cttcatcaac catactctac acctctaaca ttctttctag ttcctctaat
     5821 ccatccacta tctctttctc taacacctta accctaccta taataacact aggagcatca
     5881 taaactactt cctcatacac tacctcttta tacttattaa tagataaatc atacccattc
     5941 tctcttatct cttccacatc aacaaaaaag ctttgctctg ttctttttct atctacttcc
     6001 ccatcaagat tatcaaatct cttaataata tcctctatat cactatcctt tatctcattt
     6061 ctcttatcat ccaaagaata accatcagct ttcatatcat agaaccatac cttatcagtt
     6121 ccacctgtcc ctgttttagt aaatatcatt atagctgtag atacccctgc atatggctta
     6181 aatacaccac ttggcattga tattatagct tccaatttat gattatcaac tatttctttt
     6241 cttatcgatt tatgtccacc tgttgaacca aacaatactc catctggcac tatacatgcg
     6301 catcttccac caatttttaa aactcttaaa aaaagtgcca agaataataa ctctgttttc
     6361 tttgtcttag tcacttttaa taaatctgct gacactgctt cataatctaa acttccctta
     6421 aaaggaggat ttgctagcac caatgtaaac ttatctttat ctgtattaac ctccgataaa
     6481 gaatccttat actctatatt aggattgtca acgccatgca acatcatatt catagcacct
     6541 attctaagca tagttctatc catatcaaat ccatagaaca taccattatt aaaatgctcc
     6601 ttcagccctt gcactaagaa aagacttgaa tgattatccc ttaagtactg ttgtgatgat
     6661 actaaaaaac ctgccgaacc tgctgccggg tctactataa tatcttctgg tgttggttta
     6721 ataagtctta ctatcatatc tattatgtgt cttggagtcc taaattgacc attagtacct
     6781 gctgtagcca ctttggataa taaatactca tacaaatctc cctttgtatc tctatcctta
     6841 cttaattcta aattactaat accatcaact attttagata acataagagg cgtaggaatt
     6901 ttaaatattg catctcccat gtactttgca taagctgatt ctttatttcc atgtaatttt
     6961 tttatgaaag gaaacacttc attttgtact atttcataca ttttttctga gtttccttca
     7021 ttagaaaact tactccatct taaatgttga tgttcatcat caaatatctt ttcaaaagaa
     7081 acacccaaaa gagttgcttc tgcttcattt tttgtttcta cttcatccaa tcctttaata
     7141 aataaaagat atgtaaattg ttctataact tctaatggat tagttaatcc accagtccaa
     7201 aatgtctccc aaatcttgtc tactttactc cttaattctc ctgtaatcat atatgacttt
     7261 ccctccagtg ttcataaaat aatttaaata tatattttag tcgtacgcct taatagttag
     7321 tgtcttatgt taaatccaag tttttactac acaattatta aattattttt cccatttaca
     7381 tattttagct aaaaaactaa tgcaatactc ttattttatg attattatat ataccataca
     7441 aaattatttt atcatatatc catcatagaa gtcatctacc tggataactc ttaccatatt
     7501 ataacttttt aatattaatt taaacttaga aagaaatttt ttatttaat
//
LOCUS       DAEPOG010000175         7543 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEPOG010000175 DAEPOG010000000
VERSION     DAEPOG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.175"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(127..654)
                     /locus_tag="KR432_003123"
                     /pseudo
     CDS             complement(127..654)
                     /locus_tag="KR432_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            938..1504
                     /locus_tag="KR432_003124"
     CDS             938..1504
                     /locus_tag="KR432_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0641636.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1695..3731)
                     /locus_tag="KR432_003125"
     CDS             complement(1695..3731)
                     /locus_tag="KR432_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBH0641637.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3905..4582)
                     /locus_tag="KR432_003126"
     CDS             complement(3905..4582)
                     /locus_tag="KR432_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0641638.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            5277..5462
                     /locus_tag="KR432_003127"
     CDS             5277..5462
                     /locus_tag="KR432_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH0641639.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(5503..>7543)
                     /locus_tag="KR432_003128"
     CDS             complement(5503..>7543)
                     /locus_tag="KR432_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0641640.1"
                     /translation="GIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKILNIDL
                     KNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFLNNNL
                     KSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLEYLGC
                     AKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKNGENI
                     VVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTETMDVN
                     ISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKDEKIY
                     TSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADRNETS
                     VKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKEINRL
                     EAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNLSKVV
                     VVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGGESLF
                     NNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALS
                     VGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
BASE COUNT         2488 a         1187 c          825 g         3043 t
ORIGIN      
        1 ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca tataggtttc
       61 cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg tgcatagcta
      121 ttttttaatc aaattattta ttttattact tttagaacgt cttccatact ttttaatctc
      181 acgattgatt atcagatgag caagatttag aatgattcct tgctttccat ttgaaccaca
      241 tagtataaga ccagataaat tattactgta atccattata tatctttgac ttgcaaatga
      301 acccatgctg tgtccaaata gataaattgg caaatcttca ttttctttct ttataatatt
      361 tgttaaagta tacatgtcct ctacaagaca tctaaatcct tctttttcag ctaaatgtcc
      421 aacattttct attatctttg cagtttttcc atgacctcta tggtcattga tatatactat
      481 ataaccattt ttagtaagta ccttggcaaa agtctcatac ctttgtgcag tttcagccat
      541 tccatgtgca atttggataa ctgcctttgg tttttttata ttttcatctt cccatttata
      601 tgtatatata tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat
      661 ccctccagat tgattttatt cagtatatta atttatatat tatattacaa gtattttcaa
      721 gtcaaaatct ttctaaaaaa tttaagaatt attaattcct catagaaatt atcattggta
      781 catagaagtt tttacataca attatacaat aatttaatta atagttacat aaaaaagtaa
      841 tactttaata tattttgaca ccatattgac attagaatta attcatgata ttctgttttc
      901 gacaaaacaa cttctaaaaa taaaggagat atatttcatg gattattcaa atgaactaaa
      961 agagctcttt ttaatgaatc aaacatatgc aactcttttt acccttacaa acaaaataca
     1021 aatagaagga gataaatatt ttggtatact tacttcaaga cagtatatga ctattttatc
     1081 aattcttcat ttaccagagg aagaaacaac attaaataat attgcaagaa aaatgggtac
     1141 aagtaaacaa aatataaata gactagttgc taatcttgaa aaaaatggat atgttgatgt
     1201 aattccaagt cctcatgata agagagcaat taatgttaaa gttacagact taggaaaaaa
     1261 ggttatggta acatgtagta ggactggaat aaactttatg gcagatgtat ttcacgaatt
     1321 taccaaagat gaattagaga ccttatggag tttactaaaa aaaatgtatc gttttaatgg
     1381 tgaagaacaa gatggctttg aagaagatgc taattttatg gaatatgaag aaatagataa
     1441 aattaaatct gaagcacttg aagaatttgc aaaaagaaga aatagggtaa ataaaaacga
     1501 ttaatactat cgcatctata ataccacttt tttatagtac aatattttat ataactataa
     1561 tgtcattttt attttttaac aaaacaatca ttacatagat taggtgttct taggaactct
     1621 ttttctgttt tttgtacaaa aaaaggagat gattagactt ttattttaaa gcatctcctt
     1681 tttcgctatt atgattattc tactcctaaa atcatatctt ttaatttttg taaatcatta
     1741 attggttgac tacaactatt actttgacac acgtaataac tagtcttatc atccttcaat
     1801 ctatagttat ttaaaaaacc tataatagtg ttttcttcat tgtatttctt tgctaaaaaa
     1861 gttgcatttg gtataaaatt ttcacttatc aattctttaa atgcaattaa atcagaatct
     1921 tccttaaaaa tacatataat ttccttagta gaataaagtt caaacatcaa tgaaagcata
     1981 taaaagctgt agccagttgg agaactcttt acattatcca catacagttt taattgttta
     2041 tatgacattt cttcaagtct attatctcca gtaattttcg caagtcttat aagattgtat
     2101 aattgaacag aatttcctga tggaatagct ccatcataca aatcttttgg tcttgctatt
     2161 aaattttcac tatctttacc atatatataa aatccacttt tttcataatc ccaaaaaagg
     2221 ttgatacagc tttcattcaa atttaaagcc ttttctaaat atttcatatt aaatgttgac
     2281 tcataaagct ctatatacgc ccagattaaa aaagcataat catctaaata tgctaaatag
     2341 tcggaacttc catctctata tctagctaat aatctacctg actcatttac aagattatta
     2401 tttataaagt taagacactt atttgaatac tctaaatata tgtcattttt taaagtacta
     2461 tatgcctttg ttaaagcaac tatcatcaat gcattccatg atgtaagtat cttgtcatct
     2521 ttatgcaaag atgttctttc ttttctatat tcaaatacct ttttactaag gtcagctatc
     2581 ttttcattat gtctttcata ttctttattt tttataagat taggaatact tttaccttca
     2641 aaatttccac tactagttat atcaaaataa ttattaaaga aagttccatc ttcttcaccc
     2701 aaaacttcaa ttatctcaag aggattaaat gtataaaatt taccttcttc accttcacta
     2761 tcagcatcct gtgctgaata aaacccacct tctttatcct tcatctctct aactacataa
     2821 tctatagttt ttatagcaat ttctttatat aactctttct ttgttatttt atatgcatct
     2881 aaaaaagcta tagtaagcat agcattatca tatagcattt tttcaaaatg aggagccaac
     2941 cactttttat cagtagagta tcttgaaaac ccaaatccaa tatgatcaaa caagcctcct
     3001 ctatacattc catctaaagt tttttccacc atttttaaca catctttatc tttttctatg
     3061 ctataatatt tcattaaaaa cataagattc tgaggacttg gaaatttagg agcattacca
     3121 aaaccaccat acttttcatc ataaatggcc ttaaatactc taactgatga actcaacatc
     3181 tcttttgata aatcaccctc agtatttttt acaccaaaat catccttaag agcttctatt
     3241 atttcatctc cagattttat taatatatcc ctacttgtat tccatttttc agatacattc
     3301 tttaataaat ctataactcc aggtctatta taccttgaat attttgggaa atatgttccc
     3361 gcaaaaaaag gttttttatc tggtgtcatt attatggtca ttggccatcc accactacct
     3421 gtcatagctt ggcaaactgt catatacacg ctatctacat caggtctttc ctctttatct
     3481 acttttattg ccacaaagtt tctattcatt atttcagcaa cttcttcatc ttcaaaagat
     3541 tctttttcca taacatgaca ccaatgacat gttgaatatc ctacacttaa aaatattggt
     3601 ttatcctctt cttttgcttt tttaaaagcc tcatcattcc aactatacca gtttatagga
     3661 ttataagcat gctgtaaaag atatggagat ttttcattta ttagattatt aggttttcta
     3721 ttactgtcca aaataccacc tcatttgtta ttttattata tgttttgcaa taaacaaata
     3781 gatatacact tataataagt gtatacccat ttttaactat acatatttaa tttttcatat
     3841 aattaattct tctagtaaat aatttttata actactaaaa caaaacaaca taaaaaatat
     3901 caaattattt ttttaaaggt ttttcaaaag aaggaggatt aatatctatc ttagtattgt
     3961 gatttggccc tggagtagac aaatcgaaat ataatctaga atcttctgta actccaaaat
     4021 ctctatttga acctgtatct ttaactttat ttaaagcatc tctaaataga gcattatgtg
     4081 cttcttctct gttaagtaag aaatctattg tttcttttac atatttatca tctatttgtc
     4141 tatacaagta ctcataaact accttagctc tttgttcaga agctatattt gaaagtaagt
     4201 cagctactaa atcccctgta actgtaacat aattagcagt ccaaggagct cctgatgagt
     4261 ttattaagac tggtgataga ccagttaata catgtgtttc tatttcgcct gtattaactg
     4321 cattataatc aacatcatgt ccatttaata aatttatagt ttgtgctacc atttccatat
     4381 ggctaagctc ttctgctgct atatcaagaa ataaatcttt tatttgaggg tcttttattc
     4441 taaaactttg ggacaagtat tgcatagctg cttttaactc tccatttgca ccacccaact
     4501 gttcttgcat taaaacagca tattgaggat ttggtctttc tacttttact tcttttaata
     4561 atgctttatc gtgtttaaac atatgaatac ctccgtaaat tattattcaa aattattatt
     4621 tacttaagtt gtaatattat tctaggttat ttgtttattg ttgttatttt ttacaagtgt
     4681 tggtatttca atgttttatt aaggtcagtt ttgttgttta tgttttatta tgtttaattt
     4741 aaatttattc tattatttat tttcggtggc aaaatggtgg caagtttggt tttgtcacca
     4801 tttattttta tttgtaaaaa atatagttta tcaatataac tttattgaaa aatatgtaat
     4861 atttattgac tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaag
     4921 tggggagatt agaaagaaga aaaaataaaa gagaaaacaa atttaataaa attaaaaatg
     4981 ctttctcttt cacattagtt ttaattaatc tagtactcgc aatactaaga ttgattaaag
     5041 aattataagt ttcaatccta ggaggaaaat accttccttc taggtagctt ctttctaata
     5101 ttataacaca ttttctaatt taatatgaat aataaatttt atagaatttg tatgattatt
     5161 cttggtatca ctatagtttt aaatattatt aatataataa taagttttac ttttaaagct
     5221 cttattgctt tggtatttac tttattattg ctggcattta ttaatagtag aaaggaatga
     5281 tattattgga gcaaagaaaa cttagaattg gatttaataa aagtggcaaa ggctctttta
     5341 cacccagatt gatacttcca atgtcatgga ttaaagagat gaatatctct cctgatgaaa
     5401 gggatgttct tgttactttt gaggatggaa aaataattat tgaaaaaact gaaaatgaat
     5461 aaaaaaggag gtagtaacta tttccagtta ctacctttca atctatttaa ctaagttttc
     5521 tacttcatta aatgcttttt catttccatt tccaccaatt tgagttatat ttttaaactt
     5581 tttattcttg attaactctt tttggctatc atctaatcca tttccaacta atactactgg
     5641 agattcagtt ttacctgcaa gcactcctac tgataaggca tctactaaat cgtcttgttt
     5701 attcattcca ttcttagcta catataagtc attaagtgta tctttgctat agaaatgact
     5761 tattactttt gagttagttt ctgttctatc agaacctgct attttagtta cagatggtaa
     5821 tgtattattc acttctttat tattaaataa agattctcca cctattacat atgattttga
     5881 tatgttttta ttagctatta agtcttttat atctttcata tcatcatttt gattagtaag
     5941 aagaattggc atttcctctt ttgctgatat agctcccata cttactgcat cagctagtcc
     6001 cttttctcca tttaccacta ctacttttga caggttagaa tttttgtcta attcttttgc
     6061 aagttttatt gatgtttcat atctatcatt tccagatatt ctaattacat ttaatccttt
     6121 tttctttaaa tcagacacta ctttttcatc aacagacttt aatccaccca ctatgtacac
     6181 attttttgct tctaatctat ttatttcttt ttctgttaat ttatttaaat tattattttt
     6241 agtaagtaat attggtgcat ctttagactt agcaaatggt gttgctgata aagcatccga
     6301 tatgctagag tcatttatta acactatatt atcagcttta ttccatcctt tttgacttat
     6361 tttcactgat gtttcatttc tgtctgcacc tgttattttt tccttagatg gtgctatatt
     6421 aaaatcttct ttacttgaag ttccaccaga agaaccactt gatcctgttg attttctttt
     6481 ataagttgct ataaaatcag tactatcatc aaaaactaat ttacttacct gctctgatgt
     6541 atatattttt tcatctttat tgttcctcca accactaaac tcatagtttt ctctttgagg
     6601 attaatttga atttcattta tactagaatt atctgatatt tctttttcat taattatact
     6661 tccatcataa tctatatatc tgattctaca tttcactaat ttatctattg atatatttac
     6721 atccatagtc tctgtatcat ttactggacc atattcatat tccaaactat aacttatgct
     6781 atttggaaag tcagaaagat tttcccatgt tagaaaacct gttgaaatat tataattttg
     6841 tggatataat tcctttaatt tcgaattttc aattcctata tctacaacta tattttcacc
     6901 attttttata atgctttttt ctatatattg accactacat tccagtgaat ttaattcttt
     6961 atttttactt atatctattt ttgttaaact atttttccca caaagtaaag aatctaattt
     7021 tatatttgta gatacatcta aatcttgtaa tttattctta gcacatccca aatactcaag
     7081 acttgtaagt ccttttatat ttaatttact caggttatta cctgaacaac ttaaattttt
     7141 caaaaaaaca ttattactta aatcaaggga tttaagttta ttgttagtac aatttagaga
     7201 tttcaacttt ttattatgac ttaaatctat actttttaaa ttattattaa gaaattgtaa
     7261 attttctaag ttcacattat tacttaaatc tatctctgtt aattcattac tagtaaaaaa
     7321 taaacttacc aaatttttat tatttttaag gtctaaattt gataatttat ttgcatcaca
     7381 atgtaattca ttaataagat tattgttttt taaatcaatg tttaatattt tatttccact
     7441 aatatctaga tattttaatt ttatattttt acttaaatct acactttcta gattatttga
     7501 actacaatca agcttttcta gatttctaaa attttctatc cca
//
LOCUS       DAEPOG010000176         7436 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEPOG010000176 DAEPOG010000000
VERSION     DAEPOG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.176"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..251
                     /locus_tag="KR432_003129"
     CDS             <1..251
                     /locus_tag="KR432_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0641641.1"
                     /translation="ILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDNNYEEI
                     ENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI"
     gene            483..1694
                     /locus_tag="KR432_003130"
     CDS             483..1694
                     /locus_tag="KR432_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0641642.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1886..2146
                     /locus_tag="KR432_003131"
     CDS             1886..2146
                     /locus_tag="KR432_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH0641643.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2499..4211
                     /gene="ptsP"
                     /locus_tag="KR432_003132"
     CDS             2499..4211
                     /gene="ptsP"
                     /locus_tag="KR432_003132"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH0641644.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(5003..5668)
                     /locus_tag="KR432_003133"
     CDS             complement(5003..5668)
                     /locus_tag="KR432_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641645.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            6012..>7436
                     /locus_tag="KR432_003134"
     CDS             6012..>7436
                     /locus_tag="KR432_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0641646.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQ"
BASE COUNT         3107 a          711 c         1242 g         2376 t
ORIGIN      
        1 gaattttaaa ttccagtgtt tatgataaat actttaaaat aactgctaaa aaaatgagta
       61 aaaatatata tgattactat cctaataagg ttatgaagat aaggattttt agggataata
      121 attatgaaga gatagagaat ttatcaaagc aaataatatc aattttatta aataagtcta
      181 tagataaagg aaaagtagaa aaattgcaga taaaaatgga taacttgata atggattctt
      241 taggtatata agcagttgta agtattaaaa aattataatc ctttaattgt ttatttaaat
      301 ttagaaataa aaagttagcc atataaaaaa tattaaaaat atatactttt caatattttt
      361 ttgataaaat aagaatgaat ttgtgcaatt ttatttttct ccaataacta aaaatagttt
      421 tttgaattaa tatttgctta agaataaaaa tcagaaacgt ttttgatata gtaggtgata
      481 atatgaaagg attagtttta gaaggtggag gaacaaaagg agcctaccaa ataggtgcat
      541 ataaagcttt aagagagctt ggtatagagt ttcaaggagt aactggcaca tcaattggtg
      601 ctttaaatgg agcatatata attcaaaatg atattgaagt aatggaagat atttggttaa
      661 attatgacta tactcatttt atgaatattg atagagaaac ctatgagagg tttaaaaatg
      721 tagatttcac tactaaaaat ctcaatgcag tgtttgagct tataaataaa gctagaaaaa
      781 atgaaggtat agatataagc ccattaagac aactaatgga gacaacatta aatgaagaca
      841 taataaggaa atcaaaaaaa gactttggac ttgttacggt ctggtgggat aaaaaaatta
      901 atccatatcc attgtatatc gaagatatac ctaaaggaag attgattgat tatttaattg
      961 ctagttccag tttgcctatt tttcaaacga acatgatgga tgataagttg tatcttgatg
     1021 gaatgttttg tgataacatg ccaataggac tcttggaaga caaaggttat gaagatgttg
     1081 ttgtaattag gttgattgat gattttattg ggaaaatgaa tctaaatcga catcaaaata
     1141 taaatataaa aactataata ccatcagaat atctaggagg ttctcttaat aaagatagag
     1201 ataatgtaga aaaaaacatc cgactaggtt acttagatac tatgaaagca tatgaaagat
     1261 gcactggaat taagtattat tttgatgtag agtataaata taatgaagaa tattgttttg
     1321 ataaatttag aagtatgagc aatgaaacaa tacaatatat ctgtaattta ttgaatataa
     1381 aaagagatgc ttcacttaga actgttatgg aaagtgtaat accaaagctt ggtgaggtgc
     1441 ttggattgcc aaaggacttt tcatataaag atttgtttta ctgtatatat gaaaagaaac
     1501 ttgaagaaaa taatataaac aggataaaat tatatgattt taacaaggtt atagatgttg
     1561 taaatacaaa tattgattct aatagaaatt tagcttctaa ttttgaagtt agacctaaaa
     1621 ttagtattaa aaagaacaaa aacacaaaat tattgacaaa ttgtattata aaagacataa
     1681 aaaatcaaag ttaaataaaa aacaaaaaaa aatttgtaat tatactcgaa aaaagtgtat
     1741 attagtagta taatattttt gtagcactag tgggaacaaa aaatgaccat aggacaaaaa
     1801 agtaccactt agtaccatta aagtaaaaaa caggaaaaca attttaaaaa atataaaatg
     1861 taaagtttat aggaggattt aaaaaatgga aaaagtagtt agtatcaaaa atgcaagtgg
     1921 attacatgct agaccagcag gaatgtttgt taagaaggca gctgaattta aatcaacagt
     1981 agaggttgta gcaaaagaca aaactgtaaa tgcaaaatca ataatgggaa taatgagttt
     2041 aggtttagct caaggagaag aattaaagtt agttgtaaat ggagaagatg aagaagtagc
     2101 tttagctgca atggttgaat tagttgaaag tggatttgga gaatagatat aataattttt
     2161 taaaatttga tatttagaat ctaatattac ttaaagtaaa gtaacagaga agtattaaac
     2221 atatacagac caacatttgt agatatttaa tttctttgtt actttcatgt ttatttaatc
     2281 agaagattaa gactattgtt aattgggtat aaaaatataa atattttaga aaatgaaata
     2341 agatattttt aatcataaat tcagagtaaa aatacagtaa caaacagttt ataaaattta
     2401 ataaaaaatt ataagtaaat aaaactataa aaatgtaaat tatttatata aatttataga
     2461 gtttatgtgc tagatgatat tttttaggag gtagggttat ggcttacaaa ggaataggtg
     2521 cttctcctgg tgttgcttta gggaaagcgt tagtagttga acatagtgaa ttagtaattg
     2581 aaaaaaaatc tatagataat gttgaagctg aaattgctaa attagaagat gcagttgcag
     2641 tttctaaaga agaattagta aaagttaagg aaaaggcttc tgaagaatta ggagcagagg
     2701 aagcagaaat atttgaagca catttattag ttttagaaga cccagagtta attggttctg
     2761 caatagataa aattaaaact gaaagtgtaa atgcagagta tgctttaaat gaaataaaag
     2821 agatgtttgt tagcatgttt gagtctatgg acaatgagta catgaaagaa agagcggcag
     2881 atataaaaga tgttacaaat agaatactta gacatatatt aggaattaaa gttgttgatt
     2941 tatctgcttt aagcgaagaa gttgtattaa tagcacatga tttaactcca tcagacacag
     3001 caactatgaa taaaaaaatg gttttaggat ttttaactga tataggtgga agaacttctc
     3061 atactgctat catgtctaga actttagaaa tagctgcaat cgttggatta aatgatataa
     3121 cttcaaaagt taaggatgga gattttgtag ttttcaatgg agacacaggt gaagtaattg
     3181 tgaatcctga tgaagagact ataaataaat atactgaatt aaaagctaag tatgaagatg
     3241 agagaaaagc attacagcta ttaaaaggaa aaccatctgt aactttagat ggaaaacatg
     3301 tggaattagc tggtaacata ggaacaccta acgatataga aggtctaata aagaatgatg
     3361 ctgaaggtgt tggtctttat agaacagaat ttttatacat ggatagagat tctttcccaa
     3421 cagaagaaat acaatatgaa gcatacaaag ctgtattaga aggaatggat ggaaagccga
     3481 tagttataag aacattggat ataggtggag ataaagaatt aagttattta agtatggaac
     3541 ctgaaatgaa tccattctta ggttacagag caataagact ttgcctagat agaaaagata
     3601 tatttaagac tcaattgaga gctttatata gagctagtat tcatggaaga ttaagaatca
     3661 tgttcccaat gatttcatct ttagaagaat tgttacaagc taaagaagtt gtaaaagaag
     3721 ttttagcaga acttgattct gaaggtgtgg cttatgctaa agatgtagaa attggtatga
     3781 tgatagaagt tccatctgct gctgtaatat ctgatgtatt ggctaaacac gtagatttct
     3841 tctctatagg aactaatgat ttaattcaat acacttgtgc agttgataga atgaatcaaa
     3901 agataagtta tctttacaat caatttaatc cagctgtttt aagacttata aagactgtta
     3961 tagacaatgc acataaagaa ggaaaatggg caggtatgtg tggagaatct gccggagacc
     4021 aaaaaatgat accaatactt ttaggtatgg gattagacga gttctctatg tctccaatat
     4081 caatacttcc tgctagaaag ttaataactt cagttaaaga gtcagatatg aaaaaattag
     4141 ctgatgatgt acttaatatg ggtactgctg aagaaataaa aagctatata gaaaagactt
     4201 ttaatatata attaatgtta tatagttact aaaattcatt tttctaaata acaaaattat
     4261 attttacgaa ttaaaaagga gatgtttcaa aataaaaatt tgaagcatct cttttttata
     4321 aaagattact aagaattact taatctttgt aatagtcatt ttgtgaaaaa gcaaaactgc
     4381 attgtgacta tagtatatat ggatgttatt atcaataaaa gtactattaa aatgaagatt
     4441 cattctcaaa tgatatcaaa agtagttgtg atggagtaag ataatatttt gtgatttaat
     4501 agtaaaaatt aaaaaaatat ggtattgaaa aataattaaa agtatttaat aatagaatta
     4561 gggaagaata agtttataag taaaaataat tgattactag ttaataaaat aaatgtgata
     4621 tccaaataaa tcctaaatct aatttgagtt ttaatataga tagtctaaag tttgattatt
     4681 aaagatttaa ttgtaagttg ttacagttga tgatattgat tagagattaa agtttagagt
     4741 agaaagttac cttaaattta aaaatgcaag ataacccaat ctaaaaatta taatcactcg
     4801 ataggtttaa ggatttattt gtttattata aataggaggg tgtctcaaaa tgaacttttt
     4861 agtttatgag gcactctctt ttatatagaa ttaaatatat tttattattt caacaatgaa
     4921 aaagaagctt atctctattt tgagacagcc tctttgtata ctattattaa tttaattaat
     4981 aatataattt aaaattataa tattagttaa gatttcctgc tttattagta aataattctt
     5041 taatagcatt gtttctggaa actaaatcgt tatcagagtt gtttgttttt ttcataatca
     5101 ctgaataacg ttttttattt ataaaaaata ttaaaatctt atcatcatca aaattgtatt
     5161 ctttatatct gtcaatagac tcataaccta gatttagaat gtttttgtct tttatactat
     5221 ctacatctaa aatatatgat gggtcagcct ttagtaatat attaattaaa catttttctt
     5281 caccagcaca gaataactcc atcttacttg atatagattt agattgctct gaagttattt
     5341 ttgcaggaat aaggttgtca ttatcatctc ttagaagata accatgtgtg tcttgttgat
     5401 atactaaatt aacctttttc ttaggaacac taatcatttt atctaaatgt acataatgat
     5461 gattttcaac aaaaccttca tttaatattt caacaaagtt attaatttta cctaggcaaa
     5521 aagattctat agattcttta cagaatttat ttgtaaatgg tattatgttt acgttttcct
     5581 catctgtatt ccatgctata gcataatcca acgaatatac ttcttcctct atatctgcaa
     5641 aattatattg tactatgttt cctttcaaaa ttactcgacc tccatcttat aatatattta
     5701 atatacttta taaaacttat attatattta aataatatca taaattggat aaatatgata
     5761 tagtaaataa gaaaaaattt gaaaaaaatc actataaatg agtaataaat ataaatagtg
     5821 tatactaatg ggatggaaaa tgtccactat taactttatt accccacata tataagataa
     5881 aattattaat ataaaatata atctatcgac aatgaataga cttttaaata aacttaatat
     5941 aaaattaatt aaataagtgc ttaaaaacga tttactttaa tgatagaatc atattaaagg
     6001 aggataattt tatggatata aagaataaaa ttaaatctat atttttacca gaaaaaaatg
     6061 actatttaga tgatgaagaa tttgagtatg atatagaaaa taatgaagaa gatttaaagg
     6121 aagataacaa agtacattta aatgatttaa gcagagtaaa atatgattac agagagttaa
     6181 aagacataga aaaccaaaca gaagagatgt gtctgctagc agtaaagcag gatggtacta
     6241 taattgagtt tgtaaaggac aagacttata aagtatgtat ggaagcagta aaacagacat
     6301 ataaagctct taagtatata acaaatcaaa atgaggatat ttgtatagaa gcagtaaaac
     6361 agaactacag agctttgtac tacataaata ataagacaga aaatgtacta attgaggcta
     6421 taaaaaatgc atcaacttat gatgtcatgg aagtatttaa atttgtggaa gatcaaacag
     6481 aagatgtatg tctagcattt atagaaaggg catctaaaaa tgatgttgca gagatattaa
     6541 aaggaataaa agagcaaact ccagccatat gtttagaagc tgtaaaaaaa gatggaaaat
     6601 ctttagctta tgtaaaggaa caaagtaata gtatatgttt agaagctgta aaagaaaact
     6661 acagtgcact atcttgtgta aaagagcaaa cagaagaaat atgtatagaa gcagtaaaac
     6721 agaatgactt tgcattgtat tatgtaaatg aacagacaga aaaaatatgt atggaagcag
     6781 taaaaagaag ttatatggct ctacagtatg taaataagca aacagaagag atttgtttgg
     6841 aagcagtaag aattgatgga agagcattac agtacgtaaa agagcaaaca gaagaaattt
     6901 gcttggagtc agttagacaa aatggaaaag tattgcaata tgtaaaaaaa cagactgaaa
     6961 atatatgtat agaagcagta agaggttcat ttgaagaact tgaaataaaa gaaatattaa
     7021 gttatgtaaa gataccaaca gaaagaatat ttgtagaagc agtaaagcaa aatggaaaaa
     7081 ttcttaaata tgtagaaaat cagacagaat taatatgttt agaggcagta agagaaaact
     7141 ataatgcttt agcttatgta aaggaacaaa cagaagagat ttgtttagaa gcagttaatc
     7201 aaagttatga agcgcttaaa tatgtgaaag aacaaacaga agaagtttgt ctaaaagcag
     7261 taaaacaaga ttatagaatg ttgaaatatg taaataatca aacagaaaaa atatgtttag
     7321 aagcagtaaa gcaaaattat agagctttag aatttgttga taatcaaaca gaaaaagttt
     7381 gtcttgaagc agtaaaacag aatagaaaag cactacagta tgtaaaacaa aaacaa
//
LOCUS       DAEPOG010000177         7389 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEPOG010000177 DAEPOG010000000
VERSION     DAEPOG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.177"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(96..5306)
                     /locus_tag="KR432_003135"
     CDS             complement(96..5306)
                     /locus_tag="KR432_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0641647.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(5321..>7389)
                     /locus_tag="KR432_003136"
     CDS             complement(5321..>7389)
                     /locus_tag="KR432_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0641648.1"
                     /translation="EADILDSRLTKVLDLFQQLNMCLHKDNFRGSITFFISKSFLGRT
                     EKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGKNIHLCKPLESDEREGLLKQ
                     VLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVSSLEDYVKAYTEDFISIIGS
                     ITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQSGLFLQDSCMELQNKIKGN
                     LDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQNFIIIRKNLEKVLVSYEVQ
                     KQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQNEELEDSITRLSAYGEEGN
                     EIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVISSVLEYFKNRKVDDNVSTF
                     MFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKINVSGKSGELVDSSMYGKNG
                     EDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKNGETGDSGSNGADAGYTSSF
                     HGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNIYFISDSEEQKNRIINHCDI
                     TVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDKTVYYTGYFTSWDYKLDSII
                     KYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNTKLSTAVSKVEENLIKEKYQ
                     GYCSEI"
BASE COUNT         2159 a         1526 c          743 g         2961 t
ORIGIN      
        1 atttctattt tttacaaaaa ggagatgatt caaattttca ttttgaaaca tccccttttg
       61 taaatttctt taaaacttat atattaatta tattttcaac aaataattgt attttcaacc
      121 aatctttctt catagcattt taaatcattt tgctttagat aattaaattc agactctaca
      181 taaataccac agcctaccaa ttcaattgtc aaattaggta tattttcctt tagtatacta
      241 gtctgctcat aaataattct ttccaataaa tcaacttgta atatagaaaa atgcttcata
      301 atctccccat cactatctat atctttcttg atttcatacc aactctttga caaaattccc
      361 ttcaaatcaa tataccctag actcttcaat atatctatac tctcgctcca tgcatactta
      421 ctaggtttat caaagtatga ttctagtttc ttcttctgct catttgtaag tgaatcataa
      481 gaaagcttct tattaaaatg tgccttcaaa gaaatcttag ccatagaata tggacttcta
      541 ccagagttta atcctgcatt tttgaacttt tcaattcctt tatcttctac tgaagaccat
      601 attgagttta ttgaatctga atcattgtat ttaacatcaa gaccatgtgt aatatctata
      661 tagttttgtg gaaatacata ttgataatag acatcttttt tatcatcata tagggagaag
      721 tatccactat catataaatg aatatgcacc tttactttta aactatcctc taaaatattt
      781 cgtctaaggt tcgggatatt tgcatacatg gttacatatt ttatggcatc aaagttttta
      841 tttaatggaa cttcaaaact aatttcatct cctgtcaaaa gctttttcac ttcttcttca
      901 ttacaatcat atagagaata tgtattttca aatcccacac gtgatttccc atctaaactg
      961 cctgttaatg ctaccttcaa aatatggtta tccgtttttt gccattggta agcatcttgg
     1021 tcaagaaact ctctaatctg tctattttgc tctttcgcct gatttataac ctcgttttca
     1081 tcttctgtaa tacctacatg gcatctattt aagatattta ctatattgct tccaaatgga
     1141 aacgtccata gttgaaaagc tgctgcctca acctgacaca aatatctaac ctgatttaat
     1201 tcaatttgat ggataagttt tttctgatat tcattcatct gattaccact acctgacatt
     1261 tgataaatca attctcccat tgcaagatta tcttttttct tatcaatctt atcagtaatg
     1321 ataatttgag tttccatcat atcctgtact tttttaaata ttatagaaat ctcctctgaa
     1381 ttttccattt ttaagcttcc aaactttgtg cttaattcag ttaaaacttc cttcatcttt
     1441 aatttactta tctcattttc aaaaatatct tgatttttaa ctgtactatc gaatttttct
     1501 acaaatggta taacagattc tttcaaagtt ttatctttaa attccaccat ttgagtattc
     1561 aattcatcta atttcttaat attattttct ttatctttaa ttgcctcttt aactacctct
     1621 attttactct caaaactaga ttttacatta agacaattat taacaccact aatcgcagta
     1681 tttataagag aacaagcaga acttccaata ctcaatgcat ttttaatttt agatgtcttt
     1741 tgctgtcttt caatagcttg tttacttttc tctagtgcag ctaacttatc tttttgctgt
     1801 ttattattgt aatttgcctc caacatacct atttcttttt tctggatttt tgtgtaaatt
     1861 tccttatact cgctttcact taacccctct tttgctcttt gaatatcgtc agctttttct
     1921 gcatagttat catcatctat agacttaact ataatctcct taatttggtc aaatttagtt
     1981 cttctttctt catcaacacc tgccaaatta taagtcattt tatctaaagc attttttcta
     2041 tcttcactta gctgttctcc aagtacaaat ctcataccat cagaatccaa tttaaaatct
     2101 gaaaaatcaa ccatcctgtt agtccattgg tattcctctt ctttagctga aagattttcc
     2161 ttcaaagatt ttgcactgtc aaatgcccct agacaagctt gaccaacacc actaaatgca
     2221 gtcagtaaac tcaatgcgct ttgtattcca gaaagaatta actgtgtttt catttgttcc
     2281 tcaagttttg cctgtttctt ttttagttcc tctatgtctt cttgctcctc ttctttcatc
     2341 tttataattt cttgaatcaa caggttcatc tttgcattaa cagaaagcat tttattctca
     2401 agttcatctt ttaaaacttc tatttgttga tttccagatt caatttgagt taggatactt
     2461 tccttataag tattgaaata tccttctttt aactcatctt tctttccttg ttccaattct
     2521 cttaaggtct tgtccagata atctgtcaaa gaacgaatat ctgtaacacg atttcctcca
     2581 atttcacttt cttcaatcat ctgcacatag aatgctgttt tttgttcaag tgcaaataaa
     2641 atcatactcc attcgctaaa attcttcttt tctcctataa aagattctat tctatcagac
     2701 tgcatcaatt tatttataag gtattcatag atttcctttg cctccttata aaattctttc
     2761 tttatattca tatcatccag agaaagcact gctttatttt tcttctcaaa actatagtac
     2821 ttctgaagta tttctctatt taattgcatc tctattttct caactgcatg aaaaatttca
     2881 taaatatgtg aaagttctaa aatatcagct tctaaaattc ccactgcaca caacagtcta
     2941 agatttctat aacctttaat tccagaaaaa ccaaatctct cgaaaaattc atagaagaag
     3001 gtatctattg acaaagaata ctcctgcttt aacaattcct tttcttttaa tgcatctcca
     3061 gagcgaccgt cttttccctc tgctccgact aaacctgaat cttcacgttt gctaattgtc
     3121 cagtccttat gtttttcata aagagcattt aatttctctc catcatctgc tacttggttc
     3181 tcatttgaag catgattaaa cacatatcta tcaaaataat agtaatttct atcactgtca
     3241 tctattggag cagaggtacc tatatatctt attgtttctt catatatacc tctaattcca
     3301 ccaagacctc ctgcaccacc agctcctcca gagcctcctt ttcctgctag accatgattt
     3361 ccatcttttt tacttatagc accttttaat aaagaagctt ttccaaggag ataaacctgt
     3421 ccagacttgc tcccaagacc tgcatttcca ccattacctc ctgtgcctcc agttccacct
     3481 tgttttcctt tttctgcttt taccacatag tattttgtac ctttaacctt agcaaattct
     3541 tttgactctt ttttagaacc tattttagca ttttccccag cttctccagt tatccctgta
     3601 tctcctcttt ttccatcttg tccatttcca ccacgtccac catctacgta aagtttaagt
     3661 tctcctcttt tttctatttc tttaaaagat aataaaatat cacctgaagt acctgcatta
     3721 ttgccatttc ttccattcat tccgtcgtaa atagaacctt ctccaaccaa tttttctgca
     3781 tccttaccag atacgttgat tgaataaatc ccttctggta tacataattt ttctgtaagt
     3841 agtacaatat tcttacctga gaagacttct ttctccatat tacagtcaat tttcaactca
     3901 caatctccat acatatatat agatgacaaa atattaagtt gataaccaga tattatttta
     3961 gtcaaatccg ataaattcac tttaggtata ggaatatata tagaaagtac attttctttc
     4021 tcatatgatt ttatatatgt acaactttga atcttctcta tcctatactc tgcatcttca
     4081 agctttttgg cttcttcttc cctatattct tcttctgcta tccaatattt gatatctttt
     4141 aatgtatctt ctaaagtatt ttttagcaat gattttaaca actcacttag accaacaata
     4201 tgagtctcac tacagtagct atttaactct aaaagaagac gtgtttcttc taatatattc
     4261 actttctgta cgccatcacc atatgaaagt tcttgtacaa actccataag ctctttcata
     4321 ttattagtcc tactacataa atctgtatac atcttatcta attgagtctt tttatccttt
     4381 agactattta tagaagatag aatggaaata gaatcttcca tacctttttt gacactttct
     4441 atataaggaa ctaaaagttt ttctaggtct ttataggtta catcaacggc tttttgtatt
     4501 tctattttac aagaagcagt aagcatcaat cccaaatctg ttttttcaaa tgtttcaggt
     4561 agatttttca tttcatccaa caacttttct ggctgtctat ctagtggatt acataattta
     4621 atagatacac catctgataa tgctacattt aataagtaca ctaattcctt acaaccgtcg
     4681 taatctttct cttgactatc taggtcttct tttgcttttt ccaatatatt ccttattttt
     4741 tcttctgtta cattaccgat tagtgtttta ggtattttat tgataagaaa gaagaaattt
     4801 tctttatact ttgcataatc accaagcata tctttcaaat ttttgcctgt ttctatcaat
     4861 aatgtatgtc tagagtccag taaggattga tagtctaaaa taactaccat ccctttcacc
     4921 tttgaagatg tagaaatagt ttcccttaca agcatagcac cagcaatcat atactctgct
     4981 ccacggttat caaaaaaacc tgggcaatca cataatgaaa aatttctatt tgcaatatct
     5041 acaacttctg gacataaagt cttagaatat acaccatttc caatttcgac agctgaatct
     5101 tttgcagtta tatatatttg tccagtctct tcatccgttt tttcttccat cacgcaacct
     5161 tttaaataat ttatacaagt actctttcca acgcctgaag cacctaagaa taacaaaatt
     5221 gacttttctt ttaagtcata ttttctaagc tcgtctcttc cttcttccaa aaggtttacg
     5281 atttcactat aaagtttatt tgccatacat ccatcccttt ctatatttcg gaacaatagc
     5341 cttgatattt ttctttaata agattctctt ctacctttga aacagctgtt gataacttag
     5401 tatttggagc attaaatcct ccgtcgtctc ctctatctcc atattcagcg tattctcttg
     5461 ctggtcctgt tttgccttca ttccaacttc cgccactaaa ttctatatga tatttaatta
     5521 tactatctaa cttataatcc caacttgtaa aatatcctgt atagtaaaca gtcttatcta
     5581 taccacttgg agaaaatgaa agtgtattat atccgtgata gccaccactt cctccatatc
     5641 cacctgaacc tggtctacca tttggacctg cttctccatc tttcactgtt atatcacaat
     5701 gattaattat tctatttttt tgttcctctg agtctgaaat gaagtaaata ttacctttat
     5761 taccagcatt tccacctctt ccggcatttc caccagctcc acctttgtca ccttttttac
     5821 cacttgtacc ttttgcaaaa taccctttaa acatctgtcc atggaaacta cttgtatatc
     5881 ctgcatcagc tccattactt ccagaatctc ctgtttcacc atttttacca tctattcctt
     5941 tagaaccatt tcctccattt gcaatcagtg ttaaacgata atttctaatt tcttctttaa
     6001 tatataagta aatattacct gcatctctac catcttcacc atttttccca tacatagaac
     6061 tatctacaag ttctccgctc tttccactta cattaatttt tacattacta cctacatcaa
     6121 gattgttaca agcaagaata atatttttct ctgatgcaaa gtccattcca aggttatcat
     6181 ccatataaat agtatctttg gcaaagaaca taaatgtaga aacattgtca tctacttttc
     6241 tatttttgaa atattccaat acactactaa ttactaagtt tggaattaat gctcttattt
     6301 caataccttt cttatcatta atattaattg tttttactat aaatggattt aaaatgtatt
     6361 taccaataaa ttctctaatt atctcattac cttcttctcc atatgcactt aatcttgtta
     6421 tactgtcttc taactcttca ttttgtgtaa ctgtaacatt tgtcaaaatt gaattaaaat
     6481 cacttacact atatacataa tcctttttat ttgcaacttc cattaatttt tctctatttg
     6541 gtaatgtaaa tacctgtttt tgtacttcat aagaaactaa aactttttct aaattctttc
     6601 ttattataat gaagttttgt tccattatct tcttacttgc cttttctata ataaattcca
     6661 tatgtacaga aggtaataaa atttccccag ttccatgttc tgtatattta cttaaagcat
     6721 caattatatc taagtttcct tttatcttat tttgtaattc catgcaacta tcttgtaaga
     6781 ataagccact ttgtttggct aatacgttga taaatctctc tatagtaaga gttgttgtac
     6841 tttccaacca gtttttaaat acttttacaa cctcatcata ttttgctatt tcttgaatca
     6901 ttgttatact tccaataata ctgataaaat cttctgtata cgccttaaca taatcttcta
     6961 ggctagatac gatattgttt tgaatttctt ttatcaacgt atctaaaact aatcttgcat
     7021 ctgcactaat tggatattgg aatgctgatg tcttatcttt aacttcatct aattctagta
     7081 cttgctttaa taagccttcc ctctcgtctg attcaagtgg cttacaaaga tgaatatttt
     7141 taccattatc ttttagcaat tcttcaaata accaagcttt ttcctctata ttactatcta
     7201 aattttcaca taattgttca tattttcttt ttataacatt atatatttgt ttttctgttc
     7261 ttcctaagaa actttttgaa atgaaaaaag ttatactgcc cctaaaattg tctttatgaa
     7321 gacacatatt taactgttga aacaggtcta gtacttttgt aagtctagaa tctaatatat
     7381 ctgcttctg
//
LOCUS       DAEPOG010000178         7282 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEPOG010000178 DAEPOG010000000
VERSION     DAEPOG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.178"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            96..659
                     /locus_tag="KR432_003137"
     CDS             96..659
                     /locus_tag="KR432_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641649.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1056..2756
                     /locus_tag="KR432_003138"
     CDS             1056..2756
                     /locus_tag="KR432_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641650.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2926..3543
                     /locus_tag="KR432_003139"
     CDS             2926..3543
                     /locus_tag="KR432_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641651.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3904..5097
                     /locus_tag="KR432_003140"
     CDS             3904..5097
                     /locus_tag="KR432_003140"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0641652.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5115..6407
                     /gene="lysA"
                     /locus_tag="KR432_003141"
     CDS             5115..6407
                     /gene="lysA"
                     /locus_tag="KR432_003141"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH0641653.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6624..>7282
                     /locus_tag="KR432_003142"
     CDS             6624..>7282
                     /locus_tag="KR432_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641654.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
BASE COUNT         2928 a          750 c         1328 g         2276 t
ORIGIN      
        1 aaaatttgta aaaccacatt tattatgtta gaatatctta tgatattaaa ttgtaagtgt
       61 ataatatcat ttagttacta gattgggggg aaatgatgaa taaagtctat attgtaacaa
      121 catatacagg aactatatta tcttacttaa taaggaatat aagtaaaaag ctgtatacac
      181 atgtgtcaat atcattaaat gaaaatctaa aaccaatgta ttcttttgga aggctaaatc
      241 caaggaatcc atttattgga ggatttgttg aagaaaatat aaatcaagga ttgtatgcta
      301 taagaaaaaa cactgtgtgt agggtatatt cgttagaagt agataattta caatatgaaa
      361 atctatataa aaatataaaa ttaattagtg attatagaga agattactat tacgatacaa
      421 tggcattaat atatttacta atgaatattc agagacaatc tgaatataaa tatgtatgtt
      481 caaactttgt tgctgacatg ttagaaaaaa gtgagataga cattttaaat aaacaagcat
      541 ttgaagtaac gccaaatgat ttttataatt tatctggctt aaccttagag tatgaaggtt
      601 tattatcaga atataattct agacaacatt cttatgtatc aaaaatcgca aacatttaaa
      661 aacttgattt agagtttttc atataaaaaa atattgctag ataattaggc tgaagatagt
      721 agtttaactt cgataggatt aagaatttgt aaatcttagt tagaatactt tcttcagttt
      781 ttaaatttct gctaatattt gtatttttat aatatttttg gatatatata atacagaata
      841 gtgcaaaaat cccactcctt taggtgcggg atggatagca caaacagtgt ataaaaagta
      901 tttatacata cacgtataat gttaaatata gttgaataag cagaactata aatatataaa
      961 gatatcttaa tatataataa atatgataaa tatttatttt ttgtaaatat atggatataa
     1021 taataataat aacatttttt agagaggagt attaaatgaa acgatatctt aaaacatatg
     1081 gagttgcatt aataattgta ataatttatg catttattaa attacctgta ctaagattag
     1141 attttacatc tttattttca gtaggaataa ttttctttgt tgtagcagga atactagata
     1201 tgatgttaga tagaaatgaa agagcatcta aaatggctaa atataatttt tcaatagcaa
     1261 tagctttgct tgtgtatatt gtagtagttc cctttataac ttctacacct gtacttcatg
     1321 caaaatcata tagagaacta ttgggaaaag taactgaaag taaatttact gatgacatta
     1381 gccctgtcag tgtaaatgac attcgtcttg tagatgaaga tatggcaatg aaattaggtg
     1441 acaaaaagct tggagaagtt cctgcaatag ggagtgtatc caaattagga aaatttcata
     1501 tacaaaatgt agatggagag ttgtactggg tagcgcctct tgttcacaga gacataatta
     1561 aatggataac atctctaagt ggtacaagtg gttatgtaaa agtatcagct agtaatcctc
     1621 aagacgtcca attagttcaa gaaatagatg gaaagccaat aaagatagtt taccaaccag
     1681 aagcctactt acatcaggat ttacaaagac atttatacat acatggtatt gtaaatgttg
     1741 gtatgacaga tttcactctt gaaataaatg atgaaggaag accttattgg gttgtaagtt
     1801 tatatgaaca taaaattggc tatggaggag caaatgcaac aggaatagcc actgttgatg
     1861 cagaaactgg aaaaataaat gtttatgatg ttaaaaacac acctaaatgg gtggatagaa
     1921 tacaaccaca aagctttgta acagaccaga ttaaagactg gggtgtttat gttaatggat
     1981 ttttaaactc tgttatatca gaaaagggag ttttagtacc aacagaaggt acatcattag
     2041 tttatggcaa tgataacagg tcttattggt atacaggtat aacatcttca ggtggagatg
     2101 aatctacaat tggatttatg ttagttgatt ctagaacaaa agaagctaga ttatataaac
     2161 aaccaggagc tactgaaacg gctgcaatga aatcagctga aggtaatgtt caagaaaaaa
     2221 attatgaagc gacgttccct gtaatgtata atatattggg tcagccaaca tatgtatcat
     2281 cattaaaaga taaggctggt cttgttaaga gagttgcatt tgtatctgtt gaagattata
     2341 atgtacttgg aataggtgaa gataagaatg aagcattaag aaactataag gatgcattag
     2401 aatctaaggg taatgattta aaacttgata atgatttaaa agatgaggta ttagaaggca
     2461 cagtgacaag aataagccca gatgtgcgag gtggtaacac taattattat gtaactttag
     2521 attcaaataa agatataata tttagggcta cttcaaaagt atctagcgaa ttgccattga
     2581 ctcaggttgg tgataaagtc aaaatatcgt attcaaaaga agaatctggt gtaatagaaa
     2641 tgtcagaatt tgataaccta aatattgcaa actttgaaga taaaaaagca aaagaaaaaa
     2701 caggaactga tgaaaataca gattccaaag attctgaaaa tcaaggtaat gtataattta
     2761 aataaaaaga ttgataatag tatttaacat agtgatagct aatttttata gaatttaatt
     2821 ctataaaaat tagctatttt attttaattc tttgtaaatt taatgtcata aaattaatat
     2881 aatattagta caactttgca aataatactt caaggagaat gtactatgac gaatatggaa
     2941 aaagagatat tacttttaat tagcagggtt agatgtgtta cagagtctca atttagaaaa
     3001 atttatggag accaaaaaag atacaataaa aaaaatttta aaaaaacttt gagaaaaatg
     3061 tgtagtgaat atacactaaa aaaatatcct tgtgatgtgg attattataa ttataaagac
     3121 ctctcttatg tatattattt aaacggtagt aaacttttta agggagatga tttaataaag
     3181 gctctagtag gatcagaatt agttgttaga atcaatactg caggttgtga tgtaaaaaga
     3241 ttttaccgaa atgttagtgt agatgatgtg aaatacgatt tatttattga atatacagac
     3301 caatttggta atataaagca agttttagtt gatgttgact taaataatga aatagacatt
     3361 tctaaatacg aaaatctttc taaaaaaata gataaatcta caattccatt ttttaaagta
     3421 ccaaacgttt tggtagttac accagataat ctatccgaat taaatgattt agagagatat
     3481 gataatgagt atagcataaa ttttatagat ttaggattaa ataaactatt taattgtata
     3541 taagctaact ttagaaaaga aaactatcga tttgccacat tttgtaaaat aattgttgca
     3601 atattatata gaatatgata aaactatatt aataaattaa ttaaaaataa tgagcctagg
     3661 gtagaggtag ctgtattcaa tagtattcaa taggagctgg taagcattga taattgatga
     3721 aagggtatat agccgaagaa attttgttga cggacaaatt ttctggactt atgcagaata
     3781 tgcataaggc tgtcactttt aaagtggtga gctacaaggt tacttggtct atgaaattta
     3841 cacgtccaaa gtataccttt gggcgttttt atttatcaaa atttagcttg agggggagaa
     3901 agcatgagta taattgttca aaaatatggt ggaagttcag tagcagatac tgaaaaaata
     3961 aaatcaatag cagaaaatat aatagaacga agaaaagaaa atccacaaat ggtaatagtt
     4021 gtgtctgcca tgggaaaatc cactgatgaa tatataactt tagcaaagga attgagtaat
     4081 gaacctagta agagggaatt agatgcttta atgtctactg gtgaaatgat ttcagcatct
     4141 ttattatcaa tagcattaaa tgcacttgga tgtaaagcta taagttataa tgcttatcag
     4201 ttaaatataa aaacgagtgg tcttcatgga aagtctcaaa ttgatgatat aaacgtaaac
     4261 agaattaaaa atagtttaga tgaaggaaat gtcgtgatag taactggatt tcagggaatc
     4321 aacgaagatg gtgatgtaac cactcttgga agaggtggtt cagatacgtc tgctgtagct
     4381 cttgcggtta aattaaatgg aaaatgtgag atatatacag atgttgatgg aatttatttt
     4441 acagacccaa gaaaatattc aaaggctagt aaacttgatg agattgaata tgaagaaatg
     4501 cttgagttag cgagtttagg agcacaagta atgcattcaa gaagtataga gcttgcacaa
     4561 aaatatggtg ttgaaatcta tgtcggacgt acatgtggta cagaaaaagg aacatatata
     4621 agagggggaa aagacatgaa attagaggat aaagtaataa caggattagc tactagtgat
     4681 gatgacagtt ctataacaat aaaagatttt aaagctgaaa atatatctag cttatttgaa
     4741 gatatagcaa ctataggtat aagtgtagat atgataagcc aaactgcacc tattttagat
     4801 aagataagtg tatcatttac agttccaaaa gaagagttgg gagaatgtaa aaagatagta
     4861 tctaaatata cagatgaaga acatgtagtg attgataata atatcactaa attttcttta
     4921 gtaggtcttg gtatgaaaaa tacttctgga gttgctgcta aagtatttaa gatattcaat
     4981 gaaaatggta taatgataaa acttataact acatcagaga ttcgtataac ttgtgctata
     5041 aattctgatg ataagcaagt agctatagaa aaaatagcag aagtatttaa tatataaaat
     5101 ttggggaggc tattatgaaa cttcatggaa ctatgactgt aaataatgat gaattatata
     5161 ttggaggagt gagttgttta gaacttagta aaaaatatca aactccactt tatgtatttg
     5221 atgaagcttt agttagacaa aattgtagag aatatataga atattttaag gctagagagg
     5281 gaaaaaataa ggttgcttat gcagggaaag cttttttacc tctttacatg tgtaatctta
     5341 taaatgaaga acaaatgtgt ttagatgtag tttcaggagg cgagttatat acagcttata
     5401 aatcaaattt tcctatggaa aaagttttct ttcatggaaa caataagact aaagaagaaa
     5461 taaacatggg attagattta ggtgtaggta aatttgtagt ggataatttt tatgaattag
     5521 atttgcttga agcattatgt gaagataaag gtaaaactca agagatatac tttagaataa
     5581 ctcctggtat agaagctcat acacatgatt atataaaaac tggacagata gattctaaat
     5641 ttggatttgc tttagtaaat ggagatttat ttagagcaat agaaaaatta agtgaatata
     5701 aaaatattaa acttgtaggt cttcacgctc atataggttc acaaatattt gatgtagaac
     5761 catacataga tacagtggat ataatgatga acttggtaaa agaaataaaa gaaaaattca
     5821 acatacaact aacagaaatt gacttaggtg gaggagttgg cgtttattat agcgaaggag
     5881 ataaacctaa aactataaaa gagttttgtg aaaccataat cactaaagca gaaacttcat
     5941 gtaaagagct tggaatagaa gtacctacat tggtaataga accaggaaga tcaatagttg
     6001 caaatgcagg aagtacattg tatacagtag gttctataaa agatataaaa gatgttaggg
     6061 tttatgtaag tgtagatgga ggtatgacag ataatgtaag accttcgttg taccaagcta
     6121 gttatgagtg tagtattgtg aataaaatta atcatgaggc tatgaatcat gtaacaatag
     6181 ctggaaaatg ttgtgagagt ggagatatat taattggaga tataaacata atggatatta
     6241 aaagtggaga tatacttata acaacatcaa ctggtgcata tggatattca atgtcttcaa
     6301 attataataa aataccaaga gcagcagtag tatcagtatt tgatggaaat gataagataa
     6361 tttgtaagag acaaagctat gaggacttat tagctttaga gatataaaat ggaaaagtat
     6421 ataataaaaa ttaaatatta aagtttatta tgagcagatt tgctaggata tgtatctcta
     6481 aattaatttt tggtttattg tatcgttcgt agcatatctg cttatttttt gttttactaa
     6541 caaatttctt gtaataaata taaaatattt taaaaacttt ataattatga taaaatttaa
     6601 agtatatgac aaggagggat ttaatgagag gaagagtaat tttattaata tcgatattat
     6661 ctatatttct tgtaggatgt tcatttaata aaaaagatga aataaattta gtagagcaag
     6721 gaaaaacata tttagaaaaa cataactata aaaaagctat ggaatcatta tcctctgcac
     6781 tggaagaaga tagtactaat gagaatgcaa gagctatgta tatgcaagct atgagaatgt
     6841 caaatatgac ggagtttgaa gaattaaaaa attatgaggg tgcaatcaag gaacttaaac
     6901 ttatagagaa aatcaaaaat ggttcctctg ttattaaaga agaggcaact aaaaagaaag
     6961 aagagttaac taaattagaa aaagaacaaa acgaagcaga ggaacaaaga aagaaaaagg
     7021 caaaagatac ggctggagaa gataggtata gagttgaatc agatgcaagg aaatcttctt
     7081 atagtggaag tagtaaaaat aataaatata gttctaaaaa taatgataaa aaatcagata
     7141 gtaataagtc tgacaataat aacagttctt ctcaaaatca agaaaagcca aataatagta
     7201 atccaacacc agcaccaact ccacaagata ctactggtgg ttcagatagt ggtggaaatt
     7261 ctcaaaactc aaataatcaa ta
//
LOCUS       DAEPOG010000179         7222 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEPOG010000179 DAEPOG010000000
VERSION     DAEPOG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.179"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..433
                     /locus_tag="KR432_003143"
     CDS             <1..433
                     /locus_tag="KR432_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH0641655.1"
                     /translation="YTFDSKTKKFTKTKEKKQKKDVTNTEKEVAISKDSNNKKEVKNG
                     DENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKNDKYLSLYKKKVNKTMVKNIL
                     KKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEFDN"
     gene            complement(544..1029)
                     /locus_tag="KR432_003144"
     CDS             complement(544..1029)
                     /locus_tag="KR432_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0641656.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            1281..2504
                     /locus_tag="KR432_003145"
     CDS             1281..2504
                     /locus_tag="KR432_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0641657.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            2508..5642
                     /locus_tag="KR432_003146"
     CDS             2508..5642
                     /locus_tag="KR432_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBH0641658.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            5676..6974
                     /locus_tag="KR432_003147"
     CDS             5676..6974
                     /locus_tag="KR432_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBH0641659.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         2867 a          937 c         1216 g         2202 t
ORIGIN      
        1 atatactttc gattcaaaaa ctaaaaaatt tactaaaaca aaagaaaaaa aacagaaaaa
       61 agatgtaact aatactgaaa aagaagttgc tatatcaaaa gattctaata acaaaaaaga
      121 agttaaaaat ggtgatgaaa atcaatctct tggcaaatgc cccatttgtc aaggtgatgt
      181 actagaattt gacaaaggct ttgcttgtaa aaatcataaa gaatgtaaat ttgttatttg
      241 gaaaaatgat aagtatttaa gtctttataa aaagaaagtt aacaaaacaa tggtcaaaaa
      301 tattttaaaa aaaggtgaaa ctaaagtaaa atctttaact gctaaaaacg gtaataaatt
      361 tgatgctaca ttaaaatatg ataaaaatcc agataccggc tatttcaatt ggaaaataga
      421 gtttgacaac taaatattca taagaaaaga aatagcagta gtataataat ctatattata
      481 ttactgctat ttcttttctt atgaagtttt aactttttta atagtgactt ttacaatcac
      541 actctaacct aatttcttta ctatttctga taactttaat gtatgttcat ctaataaagt
      601 tagttcctct tcagaaagat tagataattt ttttactaga gattctttga gaatagcttt
      661 tactgcatca aataatttaa atgctttatc tgttaattca accctaagaa tccttctatc
      721 ttttgggtca ggaaatttat tgactaaacc atgttccact aatctgtcaa taatcggagt
      781 catatttgat tttaaaattc ctaaattaga agctatttga gaaattgatg atgaatttga
      841 tgtcataaga taaagaatta ccttagcatg tgatggagcc attggacatt cttcaaaata
      901 agctttcatt atatcagagt tattttgaaa cttttttaac aaatcgcttt ctttaaatat
      961 attattttca agcacaaata aaaaatctaa aaaagagtcc gctattttaa gtatattgtt
     1021 gttatccata tttgtaacat cccttcaata gataatataa taaaacattg ttgtttaata
     1081 tagtataaat tataaactat taataaattt aaaacaagaa aaagttattg actcataata
     1141 attataaatt tataatcaaa ttagagaatt ataaattgcc aataaaggga agttttttaa
     1201 ttagtctaaa actaaactta aattataaga cactttaaat tctacaacaa gctagtgtgc
     1261 aatatataat aggaggagat atgaaaagat atttaatagt tttcttaatg tgtagttcac
     1321 tgtttttagt tggatgtgga aaaacaaaag aatctgaacc tgagaaacca atagcagttt
     1381 ctgttcaaaa agcagtaggt ggagaaattg aaaacacaaa ttctttctca ggaactacaa
     1441 aagttaaaga tgaaacagct gttactgcac agactgttgg cacagttcaa gaagtgtatg
     1501 taaaacttgg gcaaaatgta agaaaaggag atgaattatt gagtataagc agtccagagc
     1561 tagaaaatag tgtaaagcaa agtaaagcct cactagattt agcaaaagca agttattcaa
     1621 gtgccactgg tggttcatta gaggcacaag taaatcaggc aaaaacagct cttgacaatg
     1681 caaagattca atatgaggag tctcaaagaa attatgacaa taataagatt ctttatgaac
     1741 aggaagtcat tagtttagac caatttaaaa aaatagagtt ttctttagag caaacaaaac
     1801 aacagttaga ttctgctcaa agagcatacg atacagcaac ttcaaaaagt atacctcaag
     1861 ctaaagcact tgctaaaaaa cagcttgacc aagctcaagt atcatataat ttagcaatga
     1921 gtaatttaga caaattaaca ttaacatctc cagtagatgg tacaataaca gctaaaaact
     1981 ttgattcaaa ggaaatgata acacaatcac aaccagcttt tattatttct aatcctaata
     2041 tactagaagt tgatttaaat gtagcagaat cagatatagg taaatttaag aaagatggaa
     2101 atgtagatgt tataattgaa gaccaaagga ttttaggaaa aatagattat gtaccttcag
     2161 tagttgaccc tcagacatct ttatatcctg ttaaagtact tgtaaataat gcaaacaaca
     2221 aatttaaggc aggtatgtct gcacaagtaa acttaagtat tgaaaaagaa aatggtgctg
     2281 ttacagtgcc taaaaaagca atatttgaag aaaatggtaa aaaatatgta tacatagcaa
     2341 caaaagacaa tatagcaaag aaacacttgg ttcaaactgg aattgtaaca gaggataaaa
     2401 tagaaattaa aagtggtgta agtgataaag atacagtgat tattggagga ataagtctta
     2461 tttctgacgg aactaagata tttcctgtag aaaaggagaa gtagactatg aatttaacac
     2521 agacttccgt taaacgtcct ctcacaatca taatggtctt tttagtatta atagtttttg
     2581 gtggtattgg atataaaaaa atgtcaataa atctaatgcc tgatatagaa ataccagttg
     2641 taatggtcat gactacttgg acaggtgctg gtcctcaaga cgttgatgaa caagttagtc
     2701 aaaaagttga tgaaagttta tctgccgttt caaatgtaaa atctactata tcaagttcac
     2761 aagaaagtgt ttctatggta gttgctcagt ttgaatttgg aactaatttg gatgaaataa
     2821 tgaatgatgt taggtctaaa gttgatgcat tacagaccag cctcccagat gatgcagcaa
     2881 aaccaacagt tttaaagctg gatatgaatg cacaggctat aggtcaactt gtcatatctg
     2941 gaggaaatga aaatagttca caagccttaa gaaaatacgc tgaagatgta attcagccta
     3001 aaatagaatc catagatggt gttacttcag cagaccttaa aggtggagaa aaagcacagg
     3061 ttaatgtaat agctgaccca gctgttcttt caaattatgg agttagttta tccactataa
     3121 aaggtgtctt atcctctagt aataaaacat tcccttatgg ttcaattact caaggtgagg
     3181 ataaaatagt tttaagagca attgataaat tagaatccct agatgatata aaacagattc
     3241 aaattcctgt aaaaggagga aatacagtta gattagataa tctttgtagt gtagattatg
     3301 gatatgcaga caaagatagt atttatagat acaatggaaa agacagtttg gtaatagata
     3361 tttctaaaca acaagatgcc aacactgtaa aagttatgca gagtgcatac aaatacgtag
     3421 atgaattgaa taaggaaaat cctcaattta aaattaaaat tgctaatgat acaagttcat
     3481 acataagtga atcaataaac agtgtaatga gcaacttgct tataagtgct gttatcgcat
     3541 ttattgtaat atttgctttc ctaaaaagtg taagagcttc actagttgtt gcagttgcaa
     3601 taccaatttc tataataggt gctatagcaa ttctctactt tactggtgaa agtttaaatc
     3661 ttattactgc aagttcattg gtaatttcag ttggtatggt tgttgataat gctacagttg
     3721 taattgaaaa tatatttaaa tataggaaaa gtggcagatt gagtcttgat gattgtgcta
     3781 ttgagggaac aagaactgta acaaatgcaa tacttgcatc aacattgacc acgatagcaa
     3841 tattcttacc tatcatgttt acagaaggta tagctaaaat aacttttggg gcattaggta
     3901 aaacactaat agcttcacta gcattctcct ttataggtgc aattacactc gttcctagtg
     3961 tatttgcaaa attaagcaga ggaaaaaata gtcaaaagat gcaagaaaaa gatagtccta
     4021 tatttgataa ggtaagtgaa gcatataaaa aattattaag tgtatgttta agacatcaac
     4081 gcttagttgt aatatcaagt ttacttattt ttgtagcttc cttatttgga atgggattta
     4141 taggtatgga ctttatgcct gcagcagaca agggtgaatt atctatttct atagaacttc
     4201 caaaaggtct tagcctagaa tccaatgatt actatgtatc tatgactgag aaaaaagtct
     4261 cagatattcc agaaattaaa actttaataa caactttgac atcagattca aataatagta
     4321 aagcaagtat agcaattgaa ttagaatctc aaaaaaatag aaaaagaact actgatgaaa
     4381 tagaaaaaga gataattgat agagtggcta ctgtaccaga ttgtaaaata aatgtaagtc
     4441 aaaacagtag tgttatgggt ggagctggag gagcagactt cacattagaa atgaaaggtc
     4501 ctgatttaga tgtattaaaa atactgggaa aagaagctga aaaagaattt agctctataa
     4561 aaggatttag aaatgttgaa acttctctta cagatacaac acaagaagct caatttacta
     4621 tagataaaca taaggctcaa gaatatggtg taaatactgc tgaaattgca ggcatgttac
     4681 gtacagcaat aaatggtgac tccgtaacca ctgctactat tgatgattac aaagtggatg
     4741 taaacctaaa atttaaagca gatactattg atagcttaga agatataaag caactaaaag
     4801 taaactctgc aactggaaaa gaagttcctc ttggtgcttt ttcagatata aaaatggcag
     4861 atggtctaca atctatatct aggactgatg gagatttttc tgtaaatata actgctacac
     4921 ttgacaattt agatgctggt actgctacaa aactagcact agaaaaaacg aataaaatgg
     4981 taattcctaa agattactct ataggtgttg gtggtgaaac agaaatgatg aatgaatcta
     5041 tggcaagtct tggattttca atggtcatag ctatagcatt agtttatatg gtaatggtag
     5101 ctcagtttga atcatttagt aaaccattta taatcatgtt ctgtgttcca tttgcattcg
     5161 ttggagttgt gctttcgctt ctagttagtg gtcaaagttt aagtactgtc ggtatgctag
     5221 gtataatctt acttatcggt attgttgtta ataatggtat agttcttatt gactacatag
     5281 agcaacttag aaaaacaaat cgtaacaatg atttaattgg tttggtttca attggttgtg
     5341 cagcaagatt aagaccagtc ttaatgacta cagcaacaac aattttaggt atgttgccaa
     5401 cagcactatc tcttggtgaa ggtggagaaa ctatgcaacc actagccata gttataatgg
     5461 gtggtctaac tgtatctaca cttataactt tggtgcttat acctactata tacttaatat
     5521 ttgataaatg ggaaaataag ttttcagaca aaatgcctaa aatatttggt aagataacag
     5581 ataaaatagg aagtataaaa cgtaaaataa taaagaaacc aaaattgaaa gctaaagttt
     5641 aaatagaatg gagggtgatt gtattgaata aaaaaatgag caaaatagtt gcaattggct
     5701 gtggtgttgg tataataact gccaatttaa tcccagttta cgcagataat aatacttctt
     5761 ctataaacaa cgttgataat tctattttaa gtgactcaaa taaaagaaca tctgaaaagg
     5821 aaattttgac tgctagagaa gctgttagtg ctgcacttaa aaatagtgaa aaattaaaaa
     5881 tgaaatcaga agaaataaaa atgttaaaag aaaagttaga agtacaagat gagtttgata
     5941 gttttactgg aagtgacaac tcgtttccat acgaccaaat tggacttctt aaaaatcaaa
     6001 gtgaacaggc taaaggcttt atggaggacc aaatagcaaa tgatattact aataaattta
     6061 acgatttagt ttcaagagaa aatgagctag ataagataaa aaacaatctt gaaataaaaa
     6121 ctaaagaaat taaagatatg aaattaaaaa aagatttagg gttagtaaca tctttagaaa
     6181 ctgaatcagc tgaacttgaa cttcaaacac tacaaaatac tcaaaaagct aaacttcaag
     6241 aactaaaaaa caatcaagac tattttaagt tattgactaa tatagattta aataattatc
     6301 aattggataa agaatataga tttgaatctt ttagagtagg tggctcagtt gattcatata
     6361 tggaaggtaa ggttaatgaa tatttaaaat acgaccaatt gattttagaa cgtacagaag
     6421 aaagctttaa tgataaagat gagaacaaag cagatttacc agatagacct aatcttacaa
     6481 gaccagttgc tcctacaaaa ccagagcaag gtaacctaac tgatgatgaa tacaaggttc
     6541 ttatggataa gtatgaaaaa gactacaaga gatatgaaaa agaagttgaa gcatacaatc
     6601 ttgaaagaca gacttatgct gctggcctta ctacttatgc aaattatctt caacaaaaat
     6661 ttaataccga aaatggtcta gtaacattag aagacagtaa aaaagctctt aaaaaagggt
     6721 taattgatag ctatgctcaa ttacttgctt tagaagatac aattcagatt actaaaaaac
     6781 aacttgattt atcagaaaaa caattaaaaa atactaaatt aagatatgat ttaggattaa
     6841 ttactctaac tgattataaa aaacaagtgg taagtaatca agatgctaaa aatagctatg
     6901 acactcttat tgtgaattat aactcactaa aaaatggtat agaaaaacct tggatactta
     6961 atactggaaa ataattctgg taaatagaaa gattacttta acatacgttt gattaaaaaa
     7021 tagttatgca cactcgtggc tttagtcatg gaagattcag aaggcaaata atagaaatag
     7081 attagtaaaa atatatttac ttataaaact gtatactttt atagaggcta tctcaaaatt
     7141 ggatatgtcc ctgtcaagta gacagactta aaaaaggcta tctttatgcg gaatgagttc
     7201 gatattcaat cgaactcatt cc
//
LOCUS       DAEPOG010000180         7212 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEPOG010000180 DAEPOG010000000
VERSION     DAEPOG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.180"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            1534..1923
                     /locus_tag="KR432_003148"
     CDS             1534..1923
                     /locus_tag="KR432_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641660.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2668..3138
                     /locus_tag="KR432_003149"
     CDS             2668..3138
                     /locus_tag="KR432_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0641661.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            3431..4300
                     /locus_tag="KR432_003150"
                     /pseudo
     CDS             3431..4300
                     /locus_tag="KR432_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
     gene            5206..5913
                     /locus_tag="KR432_003151"
     CDS             5206..5913
                     /locus_tag="KR432_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0641662.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            6525..7025
                     /locus_tag="KR432_003152"
     CDS             6525..7025
                     /locus_tag="KR432_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641663.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         3058 a          536 c         1165 g         2453 t
ORIGIN      
        1 aaaataattt tattctttat tgacaaaata actctattag agtagaataa ggataaattc
       61 ttttcttgtc aagttttatt aaagtaaaga gtaacttgtg ctttatgcgc ttatggctga
      121 gttactcatg gtatcaaaat tttcatagaa ataatatttt ggaattattg taaggttatt
      181 aaattcttta gataattcca ttttttttat tttttagtta atatttaatt ttattattga
      241 caaaaaaatt ctactaaaat agaattatgg tatagtgttt tttaatttta agaaaaaata
      301 taatgacatc ctaaaaaagt tttaattata cgttgttctg tgtagttata atagcttcta
      361 tgtaactatt gtaagtatat tttattataa ttatttattt aaagtacgat aaatgtttta
      421 aattgaagta ttttgcacca ttgagttcta ctaaagtaga gtttttgtgt agtattttta
      481 gaaataacat atatatatat gtatttatgt tgaaatatat gggaaattta taatataatt
      541 aatgaaaaaa tttaaatttt gtaatatata aataaaaaaa ctctaaaata gaaggatata
      601 attatataga atgtttcaat taaacaaggc aaaaactata atcttaaaat taaaattaac
      661 attattctta agaagtaaga aatttaagaa gtaaaaagtt tagtgataag tggtagtaag
      721 caagaaattt agaaagtagg aagtttagtg ataagtggta gtaagcgaga aatttagaaa
      781 gtgggaagtt tagtaataat ttgtagcaag taagaaattt aggaagtagg aagtttagta
      841 ataatttgta gcaagtaaga aatttaggga gtaggaagtt tagtagtaat aatttgtagc
      901 aagtaagaaa tttagggagt aggaagttta gtaataattt gtagtaagta agaaatttag
      961 aaagtaggaa atttagtaat aatttatagt aagtaagaaa tttagaaagt aggaagttta
     1021 gtaataattt atagcaagta agaaatttag aaagtaggaa gtttagtagt aatttatagt
     1081 aagtaagaaa tttagtaagt gagaagttta gttataattt atagtaagta agaaatttag
     1141 taagtgagaa gttcagtaat aagttgtagt aaatgaaaag tttaggaagt aggaagttca
     1201 gtaataagtt gtagtaaata aaaaatttag gaagtaggat ttactgaaat acaaatattt
     1261 aaaatcaaag agtttaaaag aaaggagagc tactggacaa gtatccaaaa tagatagctg
     1321 agtacaaaat atctcataaa attaagcaat ataatacaag agaattttga cattatttat
     1381 atatatttac cgtgtacaaa tagctaaatt taggattaag attgaaaaat atcttataag
     1441 tctgagctta tataaatccg tataagatgt atttaatcga aaaattttat tatggatatt
     1501 gcttgtctat attctgagta gcaaggtttt atcatgacta taaaaaagtt gccacaatca
     1561 gaattaaaga taatgaaatt tatatggaaa tcagattcca aagtaacatc tagagatatt
     1621 gttttaggaa tggagcaaaa atatcaatgg aaacaaacga caactttgac tcttttatct
     1681 agattagtag taaaaagatt tctaaattct caaaagatag ataaatatac acattatgaa
     1741 gttttaatta aagaaaagga atacatagga gttgagacaa gagatttttt tagaaatatt
     1801 catgattctt ctataaaaag tttattgttg tcattacatg aaaatattaa tttgagcaaa
     1861 gatgatatct tacttattga agagtggata aaaaatctaa aagaagagga gaaagacgta
     1921 taagcaaacg tatttcttat agcattaata taaagtgtat gtaagtgtat ttatgtaata
     1981 tattagtcta cttaagtaat atgacaaatg attataagtg gattatgata ctataatgtg
     2041 aatctaaata aattaaatat aaattgatag tggatattct atttaattaa atttaaaaag
     2101 ctacctttac ttataagtat aattaaggta gctttttaaa tagaggtagt gtgattaaag
     2161 agaattttta aatataaatt gtgaagcttt aatatagatg acaatatata ttttgcaaat
     2221 atgacacagt gttttcgtat ttaagatgat aaaataaata ggaattaatg aagatattta
     2281 aaaaataaaa ataaagaatt aatagaagat attaaagaga tagagataaa agtgttaata
     2341 gaagatattg aaaaagtaaa aataaaggat taatagaaga tactgaagag gtagaaacaa
     2401 aagtgttaat agaagatatt gaaaaaataa aaacaaaaga ttaataaaag gtactgaaga
     2461 gatagagata aaagtgttaa tagaagatat tgaaaaataa aaataaaaga ttgatataat
     2521 atactgaaga ggtagaaaca aaagtgttaa tagaagatac taaaaaagta aaaataaagg
     2581 actaataaaa ggtactgaag agatagagaa aaaaatgtta atagaagata ctgaaaaaat
     2641 agaaataaaa aattaggagg aaaatttatg aaatatcagg tttcattatt agcagtgaag
     2701 gatattgaag tatcaaaaaa attttacaaa gaattatttg agcaagaaat tgaattggac
     2761 ttaggcaaaa atgtaacatt taaaggagga tttgctatac aagaagactt cgcatggctt
     2821 acagggatta atccagatac aattatacaa aaatcaaata atatggaact ttattttgag
     2881 gttgatgatt ttgacgaatt tattgaaaaa ctcaattatt ataaagatgt agagtttgtc
     2941 cataagccac taaaacatga atggcaacaa agagtagtgc gtatttatga cccagataag
     3001 catattattg agataggaga gtctatggaa gttatagcta gaagatattt atctgaggga
     3061 tactctgtag aagaaacttc taaaataatt caacatccaa ttgaatttgt aaagcagtgt
     3121 gaaaatagta aggaatgatt gtttaaatta taattattta gtagaaatta attgtagtgg
     3181 aactaattca agataatata taggactagt atttgacaaa tgtcataaat taaattaaaa
     3241 taaagaaaat attaataaag aaaaataagg ggaatattaa taaagaaaaa ataaatataa
     3301 tattaaaata tattaataaa tttgaatatt tataatattt ttaatttgaa gtaggaatac
     3361 ttaaacgtat aaagtttatt atcggaaatt tctatattaa tatgtagtaa ttcaatatta
     3421 ttataaagga gtggttatta ttaaggcaat tgaactttat aaaaaattag atttagaatt
     3481 taatattaaa aatattaatg atgattggag ttttatgaat tttgaaaatg attttatcac
     3541 tccagaattt agaaaaaagt acatatgatt agtattagat aatgctcaaa atattaataa
     3601 agtatacaca actacttttc ctgataaaga aataattaag caagttatag ataaagatga
     3661 aaaagatatt ttaatttttt cacatcacgc aatgggatac atagcatcag atgaaggttt
     3721 tccatttcac gacataccac tatcttatat ggaagagatg aaaaatcgta gaatttcttt
     3781 ttatgtatta cattctccac tagacaacta tagtgactat tcaacttctg ttagttttgc
     3841 aaaagctatg ggtttagaaa tagtcaaacc attttgtaaa tatgacgata aaattgaggt
     3901 aggagttata tgtaaaacat cactaaaatc aatagaagag attaaggaat taattaaaaa
     3961 atctgttggt catgatgtga aattgtatga ttatggagat tctgtattaa aagatggttt
     4021 agttgctatt gcagcagggg gaggaagtta tccatttgta gcaagagaag ttgctgaact
     4081 tggtataaat ctatatatta ctgggtttac taagccttta aaacattttg aaccagtatt
     4141 agagttccat caaatagcaa aagataattt aattaatgtt ataggtgcta ctcattattc
     4201 tacagaaaaa tttgcgtgta tgggcatggt tgattatttt aaaaatctag gtttagaatc
     4261 agagtttttg caaggaaaat attacttaga ggatttatag gacatattaa acaataaaaa
     4321 taaacagtta gtaaaaattt attttactaa ctgtttattt ttttacctat ttatccagtg
     4381 ttttgaaaaa atctatcttt attttttaaa ctaagaaaat agacttttaa ttgttgatta
     4441 gaagatttag ttttgatgtc ttttaaatta tttttcgata ttttttagtt tattttttga
     4501 tgtattttag attattattt aggattgatt agaatttata aatatttatt ttagattatt
     4561 gtaaatattt atttaaaaaa aatagaatta ttatatttat gataataaat ggtataataa
     4621 aaggtaaagt gtcaaatatg cactaaatta aataagttaa ttagtatata ggttaaagtg
     4681 gaagtaatta aaacttatta agtattagaa aatgacttct tattagagta agtatctaaa
     4741 atgatataaa aattatgatt actcattaaa gtgactatta tagagtagat aaaattgccc
     4801 ataaaaatag ctataataga gtggataaga ttactcatta aagcaattac tatagagtag
     4861 ataaaattac tcactaaaat aactataata gagtggataa gattattcat taaagcgatt
     4921 actgtggagt agataaaatt actcactaaa ataactataa tagagtagat aagatactca
     4981 ttaaggtgat tattgtagag tagataaaat tacccatcaa agttaattat cacatattag
     5041 ataagattat tcattaaagt tactatcaca gagtggataa aattacacat taaaattgtc
     5101 tgttaaagaa tgaataaaaa agagattgct caatataaga agcatataaa aaatcaaaca
     5161 tataaaattt aataaataat aactgatagg gaaaggaaag atagaatgat atttacaaaa
     5221 agcaataaat acgataagga ctttctcatg aaaaatatga tgggacctaa ttgtataaaa
     5281 atattggaag aattaacaag taagataaaa ctagaaaagg gaatgcgtat tttagactta
     5341 ggttgtggta agggtatatc ttcgatattt ttagcaaagg aatttgatgc tactgtattt
     5401 gcaacagatt tatggataga accaactgaa aactataaaa gatttaaaga gtttaaattg
     5461 gatgataaaa tatttccaat acaggcagag gcacatgaac taccttatgc agaaggtttc
     5521 tttgatgcgg tgataagtat cgattcttat cactattttg gaaacaaaga aggattttta
     5581 gacaatcata tatcacctct tgtaaaagag gggggaatac ttgctatggc aatgcctgga
     5641 ttaaaagaag attttgtgga ttgtatacca gatgaactca ttccattttg gcaagataat
     5701 atgaacttcc attcaattac atggtggaat aaactttggt ctgaatcgga gtctgtgatt
     5761 gtagagaaat gtgaagcttt aaattgtcat gatgaagctt ggaaggattg gataaattgt
     5821 gataattcat atgctataaa tgacaaaaaa atgatggaag tagagaatgg aaagtatttt
     5881 aatacaattt ctttgatagc gagaactaaa taattgtgat gaatttataa aagtataaga
     5941 taagtagaaa tataagttga atgagagtat aagaagcata gaaatataag ttgaattaaa
     6001 gtagaaggta aataaaaatg taagttaaat aaagagttta tacaaaaatg attttttaga
     6061 ttatttatac ccaatcgagt tgcttaggtc agttgggtat ttttattttt ttaaagttaa
     6121 attgcaaata ctattgtgtg atatatattt taatactaaa tgtttgtttt ataagaaata
     6181 aactaataga aaaaatagtt ttaacaatga aagcaaaaaa ggtattgatg aagattaaaa
     6241 attttatagt accattttat atattttttt atatatttta ttatacttta aatcaaattt
     6301 caaaatgatt aagtatatat acccaaaaga ttaaagaact atatttatta tactattaaa
     6361 aattatttta atagagatac aagagataga tatattggtt ttaattttat agaaatatac
     6421 aaagatattt taagctaata tatagtaatt tatatagcat tataaactag aatcatataa
     6481 aataagttaa ctacaatcat aaattaaaaa ggagaaatgt taagttgata gaaggaataa
     6541 aatttataaa ggcagaagaa aagtatattt attcgtattg gcaaactttt gataaaatag
     6601 caaaagagag gaaatatcta gctatggatg aagcctttcc ttttgaagaa acagttgaat
     6661 ttataaaaaa tactataaat aaaaacttac cacaactgtt tataattgat ttagaatcag
     6721 ataactgtat tggttggtgt gacgttttac ctaaaactga aaaagttggt tatttaggca
     6781 tgggtattct taaagagtat agggaaaaag gtatcggaag tagtctttta aaacaaatta
     6841 ttgatttatc aaaagaatat gggtatgaaa aaattgagct tgatgtattt aaaagtaata
     6901 gtagagctat tcatgtttat aaatctttag ggtttgtaga ggtaaataca atatctagtg
     6961 gttttacatg gaatgataga cctgtaaaag aagaagtaat tcaaatggag cttacactta
     7021 tataaaataa agttaaagtc tttaaaattc catatacact tgtataaaat aaagttaaag
     7081 cccttaaaat tccatataaa agaaatattt ttaagggctt tttaataatg gttcttttga
     7141 aggtagctaa atatctaaaa ttttatatat taaatttaaa gaaaatataa agatggaaga
     7201 tacaaccatg tt
//
LOCUS       DAEPOG010000181         7124 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEPOG010000181 DAEPOG010000000
VERSION     DAEPOG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.181"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            84..785
                     /locus_tag="KR432_003153"
     CDS             84..785
                     /locus_tag="KR432_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBH0641664.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1056..1874
                     /gene="larE"
                     /locus_tag="KR432_003154"
     CDS             1056..1874
                     /gene="larE"
                     /locus_tag="KR432_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBH0641665.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1929..3077
                     /locus_tag="KR432_003155"
     CDS             1929..3077
                     /locus_tag="KR432_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBH0641666.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3213..4601
                     /locus_tag="KR432_003156"
     CDS             3213..4601
                     /locus_tag="KR432_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641667.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4630..5328
                     /locus_tag="KR432_003157"
     CDS             4630..5328
                     /locus_tag="KR432_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641668.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5331..6764
                     /locus_tag="KR432_003158"
     CDS             5331..6764
                     /locus_tag="KR432_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0641669.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6786..>7124
                     /locus_tag="KR432_003159"
     CDS             6786..>7124
                     /locus_tag="KR432_003159"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0641670.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMY"
BASE COUNT         2933 a          658 c         1280 g         2253 t
ORIGIN      
        1 gtttaaataa tagtggtttt aaaaatctga caacaaaaat aaaactttca aaaaattaaa
       61 tctaaaaaga ataggagata aatatgagta ttgaagattt atatgatttt gaatgtgttc
      121 ctttgaacaa tgagattgat attgatgttt tatttgaaaa gatgatagaa gatggaaaag
      181 aaaaagaata tacaccaatt atagtcattg atgaaaaagt agggctatta agaaaaaata
      241 tagatatgat tactaaagag tatggttctc ttgaaaacta tagagagtat tgtttaacaa
      301 aatataatga aattgatgta aatcaatttt ttgattttag gaagagtgag atttcagaat
      361 tattaaagga gctaattgat ttagaagatg atttttatga ttataaacat tttagaccaa
      421 ttataaatat agatatattt catgaatata acaaagttta catagctaaa attccaacaa
      481 ttaaaccata tgaagtattt gcatacatac ctattggagg atttaatgat tgtcctagag
      541 atgaagaaca tatagctatt gcaaaatatt ggtatgaaaa gcatggaatc tttccaatcg
      601 caattggatg tgatacagtt caatattctg taaaaaattt aactaaagat ggtaaaaaat
      661 ttgatgactt atgtattgaa ttagtatttt attgtgagga tattattata caagggtatg
      721 aaaccttaaa ggctcttaag gatgtattaa aacgctctac aatagtgctt ttttggtggg
      781 attgatattc tatcatttag atagtattaa attgttagtc accaagaatc cacttacttt
      841 agttatgggg agtgtgtcaa actaggtttt tatacacagt gtagcatagt aatatgttta
      901 aattacaaag gttaaaaaaa tactttaata tttttaatat tataattaat agatttaaag
      961 gtaattataa tttataaaat aaatacttta tatgttataa tcattaagaa tgtaaaaaaa
     1021 taatatatag ttttatagtt catatttttc cagatatgga ggtaaatttt gtgaaggaaa
     1081 aagaaaaatt agataaatta aaaaaaatgt tacttgaatt aggcagtgtt gtagttgctt
     1141 attctggtgg agtagatagt aattttttgc ttaaggtcgc aaaagataca ttaggtgaaa
     1201 atgttatagc tgtgaccatt catgcaatga tgcattcaag tagggaaatt gaagaggcta
     1261 aacaatatac tcaaaatttt ggcgttaaac atatcatatt aaatatagaa aactttgatt
     1321 taaaagaatt taaagaaaat ggtgttgata gatgttatca ttgtaaaaaa tatatattct
     1381 ctaaaataaa agaggtagca aaagaacata atataaagta catagtagat gggacaaata
     1441 tggatgattt aggagactat agacctgggc ttaaggcatt gagtgaatta ggagtgatta
     1501 gtccattaaa agatagtagt ttaaaaaaag aagaaattag gtcgctttca aaaatattag
     1561 gattaaatac ttttaataaa ccatcatttg catgtcttgc aagtaggatt ccttatggag
     1621 ttgagataac agatgaaaat ttaagaatag tagaaaagag tgaagaatat ctattcaatt
     1681 taggtttttc tcaatttaga gtgagaatgc atggagagat agctagaata gaagttgggc
     1741 aggaagaact aggtaaattt tttgaaaata acaattttaa taaagttgat actaaattaa
     1801 aaatatttgg ctttaaatat gttacactag atatgtcagg atataaaatg ggaagtatga
     1861 atttaaatgt gtagattatt ttaaaaataa ataagtttta tagttaatat aaggaaacag
     1921 gaggttttat gaaaaattat acattaatat cgccatgttt ttttggaatg gaaaaaatgc
     1981 tggctagaga gattactaat ttaggatatg aaataataaa aacagaagat ggaagaataa
     2041 catataaaac tgatgagttt ggaatagcaa agtcaaacat gtggttgaga tgtgctgaaa
     2101 gagtacatct taaaattgct gaatttgagg caaaaagctt tgatgaatta tttgaaaata
     2161 caaaaagaat aaactggtct agatatatac cttatggagc tcaatttcct atatcaaaag
     2221 cttcctctat aaaatcaaaa ttatacagta caccagatgt acaagccata gtaaaaaagg
     2281 ctatagtaga aagcttaaag aaaagttatt tagaggatgg tttgcttaaa gaagataagg
     2341 agaaataccc tatttttgta tttatacaca aagataaagt aaccatttct atagatacaa
     2401 caggagatgc tttgcataag agaggatata gagaaaaggc aaataaagct ccaataagag
     2461 agactttagc tgctgggctt atttatttaa ctccctggaa agcaggtaga gttttagtag
     2521 accctatgtg tggttctggt actatattga ttgaagctgc tatgattggt ataaatatgg
     2581 cacctggctt aaatagagag tttatatcag aaaagtggag aactcttgat aagaaaatat
     2641 ggtgggatgt aagaaaagat gcttttaata agatagataa tgaatctaaa tttaagattt
     2701 atggatatga tatagatgaa gaatctatag atatagctag agaaaatgct gaaatagcag
     2761 gtgtagatga atatatagag tttaatgtag gagatgcaac tcaatttaaa agtgaagatg
     2821 agtttggatt tataatcact aatcctcctt acggagaaag attagaagat aaagacagtg
     2881 ttaaacagtt gtataaggag cttggttatg catttagaaa attaaaaaat tggtcgtatt
     2941 atttgataac ttcttatgaa gactttgaat atgagtttgg gcaaaaagca gacaaaaaaa
     3001 gaaaattgta taatggtatg ttaaaaacta actttttcca atatccgggt cctaagcctc
     3061 ctagaaataa taaataagtt ttacatgatt tatttgtaaa atataaaatt ttattaaaat
     3121 aagtaaaaca ttatgatgcc ttgaaacgtc taattattgt gaggagattt ttgtgaacaa
     3181 atttaaagat ttataggatt aggaggagtt aaatgaaagt gttaaagctt attactgtag
     3241 tagccctgat gatgttattt gttactggat gtaatataaa aactgaatct ccagaacaat
     3301 tagcgaagat acctgactat gatgatacaa aaaaagtatt gtatgatggt attgaccagt
     3361 tactaaaacc agattcatct atgatattgc cttctaatac aaaagaagta ggaaaaataa
     3421 ataaggtaga tttaaacagc gatggaattg atgaattagt agtatttgaa caaaaagaag
     3481 atttaagtaa taatgtgagt caggtagggt ttgtaacctt gagttacaat ggagaaaaat
     3541 acgtacttgg agaccatttt ttagaaaatg gcgaatcaat agaatatgct aatttttatg
     3601 atttggactc agatggatat aaggaagtta tcttattggt taaatccaaa gacaaaacga
     3661 atatgcatat atataaagta gtagataatg aaataacaaa aatatatgac ttggatgcat
     3721 catggcttca aaataaagaa gattttaatg acatgaaagt aaaaattgga tatatagatg
     3781 gagatgacaa gttagacata ttgatgcttc atttgaataa taagactaat gaaatgtttg
     3841 caagtgttgc aaattttgat gggaaaatga aaatcaaaga ttctgttaag tttgaaaatg
     3901 taaaaaactt aagtgaatta tatacaacat taggtaatgt tgcttcatct gttggaaatt
     3961 cttctgcagc tataaaaggt attgtacttg atattcctat attgaaagat aataactata
     4021 ttacgcagat actttatatg aaagatggga aaattaacaa agcttttagt gattatgata
     4081 aaactattac taaatcttat tatatacctg tagatgatat tgacaaagat aagattatag
     4141 aaatacctat agttgcaggc agtacaggta ataataaaaa tacttatagt tctaaatcat
     4201 ctgcaactat aagttggtat agatggaatg gaaaagaagg tgctgactca agtttaatat
     4261 ttacaagtca gatatattat aattataaat ataattttaa attatttatt ccaaataatt
     4321 tatttgataa aatacttgtt gaacaggaat ttgttggaga aaaagcagta tttaaatttt
     4381 actattttga ctattcagat cgtaagaatt tatttaatat agtagtagag agtaaaaaca
     4441 aacttgaaga tagaaaaaat acaggtactc aaaactctat tgtacttcaa gagagcgatg
     4501 aatatacttt cttactagtt gtaaacaatg caaatgaaat ggataagcta gatatgacta
     4561 tagacgcatt aaaagaatat ttctcattaa tatatggtta atattatact caattggagg
     4621 ttttatttta tgaaggaaaa gatacttata ctagaagatg aaattggtat tagaagtttt
     4681 gttagtataa acttaaaaag agaaggctat gaaatagttg aggcaggtac tggaagagaa
     4741 gctatagaaa aaatgacaac agaaaatgat attacaatag ctcttttaga tgtgatgctt
     4801 ccagatataa gtggtataga agtatgtaaa tttataaggg agaattttga ccaagtaggt
     4861 ataataatgc ttactgctaa agctcaagag gatgataaaa tagaaggatt tatttcagga
     4921 gcagatgatt acatcataaa accatttagt ataaaagagc ttttagtcag ggtttctgct
     4981 cttcttagaa gagtagcaaa ggatgattca agtgttaagt cgagtgaaat agtttctcca
     5041 ccatttatac ttgatataga caagagaaaa ttatttaaaa atggaaagga aattgagctt
     5101 acaccaactg aattttcaat agtgaaatat ctaatatcaa atgctaagca atcgttaagt
     5161 agagaccaga tattagacga agtatgggga actaactatt tatatgattt taaaatagta
     5221 gatgtaaata taagaaggat aagaaataaa atagaagatg acccatcaaa gccaaaatat
     5281 atacaaacta tttggggtta tggatactgt tttagaaagg aagaataata gtgttacatt
     5341 ttgaaggtct gagaaaaaaa gtaataaaaa actattttat aataattatt ataatggtta
     5401 ctctgtttga aggattgttc atgttttaca tacaaaacta ctattatgat tctgtcaaac
     5461 aactacttga atctgaaata aaatatgctg atgaatataa tgctattaca atggaaacta
     5521 caagttttga gaaaaaagta aaaaatatat ttgataaaca accattaact aaaaattcag
     5581 aatttggaat ttcaataata gataaagata aaaatattat attagaccaa tatggtttta
     5641 aaagtaaaga aaaagcaaac tatgaagatg ttaacaatgc attaaaagat ataaaaacta
     5701 aaaatttaac accttatact tatagaatac cagatacagg ggagcatgtt atgagtatct
     5761 ccttaccact taaggtaaat aatataatag aaggtgttgt tagatataca gtatcattag
     5821 atgctattga taatgcaata ttaaagcaag ctacatggct tatattagca ggaatattta
     5881 tacttattat agctatacta ataagtctta aatttgctga gactcttata aaaccattac
     5941 gagagctaaa gaaatttgca aatgaattgg ctgtaggaaa ttataatata aaattagaaa
     6001 agatgaagat tgtagatgat gagattggtg atttagcaca gacatttgaa cacatggcac
     6061 atgagattga caaaagtgaa aaattaaaag aggaatttat atcatctgta tctcatgaac
     6121 taaggactcc tcttacatct ataaaggggt ggagtgaaac cttaggttat gaaagtataa
     6181 caagagaaga actagactta ggtttaggca ttatacaaga tgaaacagaa agacttataa
     6241 aactagttga agaattatta gacttttcaa ggctttcttc agacaggata aaacttcatg
     6301 ttgatatagt tgatgttgaa ggtttaattg taggtgttgt aaatcaactt aaagttaaag
     6361 cagctgaaaa agatatatca cttttatttg aatttgaaaa tgaatttata gagaacattc
     6421 aaggagataa aaatagatta agacaggttc tcataaattt aatacaaaat tcatttaaat
     6481 ttacgagtca aggtggttac ataaaagttg tagcttctca agatgaagag ataacaacca
     6541 tatcagtaga ggataatggt tcaggtatag aaaagcaaaa tttaaataaa gtgttagata
     6601 aatttttcca agaagattat aataaggctg gtagtggatt aggattagca attagtaatg
     6661 aaattgtaaa acttcatggt ggaagaatga aaatagaaag tgaaaagaat gttggaacaa
     6721 aaataacttt taatataaaa aataaatttg ctaaacaagc ataaattctt agaaagagga
     6781 ttaagatgag aaacataaaa atgatagtag catacgatgg ttcaagatat aaaggatatc
     6841 aaaaattagg tgacaataat atgactattc aagaaaaact tgaaaatgtt ttaagtaaaa
     6901 tgactaatga aacagtggag ataatagggt ctggaagaac tgatatggga gctcatgcaa
     6961 gaggacaagt ggtaaatttt agaactaatt gtatggattc tttggataaa attcaaaaat
     7021 atttatatga atatttacca gaagatatag ttgtaaaaac tgttgaagaa gttgatgaaa
     7081 gatttcattc aagatacaat gtaaaatcaa agacttatat gtat
//
LOCUS       DAEPOG010000182         7018 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEPOG010000182 DAEPOG010000000
VERSION     DAEPOG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.182"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..285)
                     /locus_tag="KR432_003160"
     CDS             complement(<1..285)
                     /locus_tag="KR432_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBH0641671.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMK"
     gene            complement(272..604)
                     /locus_tag="KR432_003161"
     CDS             complement(272..604)
                     /locus_tag="KR432_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0641672.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            803..1924
                     /locus_tag="KR432_003162"
     CDS             803..1924
                     /locus_tag="KR432_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBH0641673.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(2052..3950)
                     /locus_tag="KR432_003163"
     CDS             complement(2052..3950)
                     /locus_tag="KR432_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBH0641674.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            4265..5041
                     /locus_tag="KR432_003164"
     CDS             4265..5041
                     /locus_tag="KR432_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0641675.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(5133..5885)
                     /gene="xth"
                     /locus_tag="KR432_003165"
     CDS             complement(5133..5885)
                     /gene="xth"
                     /locus_tag="KR432_003165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH0641676.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(6046..6996)
                     /gene="galT"
                     /locus_tag="KR432_003166"
     CDS             complement(6046..6996)
                     /gene="galT"
                     /locus_tag="KR432_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH0641677.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT         2367 a         1175 c          782 g         2694 t
ORIGIN      
        1 tttcatatct gatttaaatt tactaaactt atcattcaaa acatctgtat ttattatttt
       61 ttcctcttca atattttttt ctttagtctg caagataagt tctttggcta tagattttgg
      121 tgaaccaagt gatgctatta tctccatatc actctttccc tctataagac catctacgaa
      181 atactcttca taatctctca atatatcatt aacttcgctc tcagaaaaat ctttttttag
      241 ataatccttt aaaatttcaa gaaactcagt tttattcaag ttcattacct ccctcatctt
      301 cacttattaa aatctcaaca gcctttacaa attctctcca ctcttccatc agattggata
      361 aattctcttt tcctagtgaa gttattctat agtattttct agcaggacct tcattagatt
      421 ctagtatata tgtttcgaaa tacaaatctt ttgtaagtct tcttagaata ggatatattg
      481 ttccttcatt tacatctatt actttagaga tgttatgtac tatctcatat ccatacatat
      541 ctttcttaga tatgagagca agtacacaaa tttctaaaac accctttcta aattgtgtgt
      601 tcatatactc catcctttct ttaaatacac agtattgtgt tttacaaggt actatgttaa
      661 aacttcttat atcctcataa taacacagta ctatgttctg cacaatagtt atttttaaat
      721 attcttgttg attaaatcct tctataaatg tataatactc taatcagata tattttatga
      781 aagtggtgat aaattggcta aaatgaaaga tgataaaaca atattaatct taactgctca
      841 atttggtgct ggacatataa gtgctgcaaa agcagtcaaa gaatgtatta ttgaaaaata
      901 tagtaactat aatgttgtta tacaaaactt tataaatgca agtattccca tgatgaataa
      961 acctatggta aaactttatg aaaacaatac aaaatatact cctggattat ataattacta
     1021 ttattacttt aagaaatcgt ttgattcaag acatgattta tctcataaat tatatacacc
     1081 taaactttct gagtatattg ctgatataaa tccagatttg attatctcta catttccact
     1141 agcggctgct tgtgtaaata actttaagat aaaaaatcct gatataaata tacctacact
     1201 tacagttata acagatgttg tagatagtat ggaatgggtt tttgaaaata cagatttgta
     1261 ttttgttcct tctcctgaaa taaaaaatag attttttcag aaaggaataa atccagactc
     1321 cataaaggtt actggagttc ctgttgataa gagatttcaa attgaaagta aagaactatg
     1381 ttgtgataaa tatagactat tacttctagg tggaggaaga ggcctatttg atatagatga
     1441 ggattttatg cattggatag atgaatttat tgaagaacat agtaattcta tagaaattac
     1501 aatagttact ggtaaaaaca aaaaattata tgacaatcta actcataaaa aacctctaaa
     1561 aaatattaaa gtacttggat ttgtaaacga catgtataat ctaataagag aatgtgacct
     1621 aatgctaact aagcctggag gagcaactat atttgaagct attcaatcac aaactccagt
     1681 actcgtgaaa atgccaaaag ttggacaaga aattgaaaac gctaaattta taatagataa
     1741 gggtcttggc atgatttata gtgatgacct agaccttaaa aacatatttt ataaattagt
     1801 atcaaatgaa tttgattcta taataaactt tatgaaaaaa aatttagaag aatttaaaac
     1861 agttattcat ccagagaaaa ttgctgatta tatatcagaa ttaatagata atcattacag
     1921 ttaaattgta ctattaaaaa atttcaaaca taaaattaaa ggcaatcaag tatgctttta
     1981 cacttgagaa tttactcata gtgtttaata atacttgatt gcctttatca tatcaaaatc
     2041 aaattgttat attagtatat atttttcttg tactgcatat acagttgttg tatttcttcc
     2101 atacttctct ttaattctat ttgtaaatct gaagcagtct ggtaatcctt attttctata
     2161 gcttctctca ctctagtgaa tagtgattct ctagttaaag tatccttcat aatattgaac
     2221 tttaaatccg atattgagtt ccaaactacc tcatcaagcg catccatatt ttcttttgtg
     2281 tgtatcttga caatacttct tattttttga atcccagcct ctattattct agtttttaat
     2341 tttttttgcc acttatctat agctccaatc ttaaatgatt ctattatact atcagtaaat
     2401 acatcacctt gttttaaaga ttttaatttt gaagcatata tttctaagtt tttcatattt
     2461 tcataaactg tagccggtgg tattccaaat cttgcatttc tttcttccaa actataatgt
     2521 tcaaatacat cattttcatc tctatatgct ctatcttttt ctaaatagaa actttcttca
     2581 cctacatttt tagataactc tttttcaagt tcttttgtac taagaccact cttagctgct
     2641 gcttttatac catccaacat tgtttgataa catcctgcta ttactaaata cgtgttagat
     2701 agagggcttg gtgaccttaa ctcaaatctt acagtctttg gatttttcat atctcttatt
     2761 aatccaacaa gcactgacct atttcttgat ggaatctcat aactatgccc taaagatgta
     2821 actatgcata caggagcttc aaatccagga actaatctat taaatccatc attagaagct
     2881 gttacaattg ggtttaatac ttcataattg taaagaagtc ccataagagc cccatatcct
     2941 agttcactta gataatcttc ttttaaatct tttggagcaa atagattttt tatacttcca
     3001 tcctttaact tcgcacttac tcctacatgt gtatgtccac cacttcctgc tactccatga
     3061 attggctttg ctttaaatgt aacttctagt ccatgtgatg taaatacatc ctctataaca
     3121 tctcttacta ataattcatt gtctgctgct tgtaatggtg tagaaaactt ccaagaaacc
     3181 tctaattgct ccatagcatg gtttgttctt ccatctatac taatagaact ttgtattcct
     3241 ccgacctctt tgtgagccat ttcagggttt acacctagct tttgaagtat aattaaactt
     3301 ttctctaaac aagttcttat aataccatga gttcttttcc agtattgctc ttttaggctt
     3361 tgtgatacat ataatttctc taagtcagct ttatcctcag gagtattaac ccagaactca
     3421 agttctgtag ctgctgttaa cattatttcc tctatatcat ctactgaatc tattcctatg
     3481 ttatttatta catgtggata ttctctaaat atttcataca ttgacttttt aaagtactta
     3541 tctgcttttt gaagtactcc tctagaacat acttttttat tatcatgtat taaaaatgca
     3601 ggtatcttta atgttccaac aggtagacct acttcttcgt ctatatattc catattgtaa
     3661 tctacatacc aatgacaact tttatctggc attaaatcta ctttcgcatt atttaatgta
     3721 gctatattat aaagctcaac acttgaacca tcagtctgta tagctgattc caaaaattta
     3781 tctatatcct ccaaaaataa ttctacagga atcttttcat ctgttgcatt tcctcctaaa
     3841 tcaaccccca ttaaggaaac aaatcttata tttttattgc tttttaggat ttctcttaat
     3901 tcttcattag tgtgtttatc tttctctatt acgtataata gactatccat ttttccactc
     3961 tcccataatt tttatgtata ttttattttg ttaaatacaa gataaatttc attttcctta
     4021 cattcatgaa tttttcttgc aaattattcg tgttttattc gtgtttttat ttatattatt
     4081 atatatcaag tataaaaagt tcgcaatatt ttttattgga aaaaatatta tacatacaat
     4141 agtttaacat ttttacaaat atggggtata ctaaaataaa agtattttta tttgaaaatt
     4201 taattttatt tataatataa tctatagtaa aagaaataat aaaaaaattt aggagataaa
     4261 ctttatggaa tacataattt tcgatttgga atttaaccaa ggatttgata aaaaactcaa
     4321 taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag caattaaatt
     4381 ggattctaaa ttcaatataa tagatacgtt taatagctat gtaaaaccta caatttataa
     4441 agacatacat ccctttatta gtcgtatgac taatattaaa aattctgatt ttaatgattc
     4501 gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac aagaccctat
     4561 attatgtgtt tggggagttg gagatttaaa ggaattatac aggaatatca actatcacaa
     4621 gctaccatca agttccttac ctaaatctta tattaatata caacaacatg catctaaata
     4681 tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta tattggaact
     4741 tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg ctaaagtttt
     4801 tattaagata tacaatccaa gtatagtccc agatatatat ctttatacga gtataaaacc
     4861 taaaactata agatattcta ataaaaaaag agttgattat gataagttat ttgatgaatt
     4921 taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta acttagctta
     4981 taatatgggg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa aaaacaaata
     5041 gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact ttaaatttat
     5101 atctacagcc caatttttat cgtaattaat ttttatttta tctctaattc aactggacaa
     5161 tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc ttctaatttt
     5221 ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc attaaatcta
     5281 tatgaccacc aactataaac gccttcttta tcaggattaa aatatctata tgtgtctata
     5341 aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa acctgcattt
     5401 ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt catatctcca
     5461 cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa gtcgtcttcc
     5521 catctagttc tatattctag tctctttaat tcagattgag aatttggcgt atatactgta
     5581 acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc atgttcttct
     5641 atatttattc catacattac ttttaaaggc tctttttttg taaatatagc agtacctgaa
     5701 taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc taactctatt
     5761 tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac ttctttgaaa
     5821 aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca tgatataaat
     5881 ttcatactta tactcctcat ctaaacattg acacactccc catagctaaa gatgggggct
     5941 tttcaacaat tttattgtaa attcttaatt tacttttatt ttaacaatac tatactataa
     6001 tttcatctat aattactatt tacttaatac ctaaaaatat atgttttaat caatttttaa
     6061 cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc caagttcaaa
     6121 tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat acgattttgt
     6181 ttcaatcttt gttggatgag tgtgaataca taagttaaat ggcatatatc cattgatatt
     6241 gtataacttc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat tattatcatt
     6301 taactcacca aactctattt tatctttaaa tattattctt acttctccac tatacttggt
     6361 tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc tatctaaact
     6421 tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat aatactcctt
     6481 tgaaactaac agttcattcg taatttcagg tggaataaaa gacatagaca atatttgagc
     6541 atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga tactaacata
     6601 ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa aaaaaacatc
     6661 ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc tctctatcat
     6721 tacttcatga acaccatata catctcttgc catatcatca attataggaa atttattgta
     6781 gacagatttt acaagccatc cttcttcact ttctatttca aataatgcat cagttgcatg
     6841 agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt cttcatcttc
     6901 atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa atataacaac
     6961 atcattagta attggatcta ttcttaattc tttcataatt aacacctctt agttaata
//
LOCUS       DAEPOG010000183         6945 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEPOG010000183 DAEPOG010000000
VERSION     DAEPOG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.183"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            199..2244
                     /locus_tag="KR432_003167"
     CDS             199..2244
                     /locus_tag="KR432_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0641678.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2505..3704
                     /locus_tag="KR432_003168"
     CDS             2505..3704
                     /locus_tag="KR432_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBH0641679.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3756..4451
                     /locus_tag="KR432_003169"
     CDS             3756..4451
                     /locus_tag="KR432_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0641680.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4464..5141
                     /locus_tag="KR432_003170"
     CDS             4464..5141
                     /locus_tag="KR432_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641681.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5252..6853
                     /locus_tag="KR432_003171"
     CDS             5252..6853
                     /locus_tag="KR432_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0641682.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2783 a          687 c         1311 g         2164 t
ORIGIN      
        1 tgtttatatt ccaataatgt caaactaacc gttttagaaa ttaaattata tgtgaaaaaa
       61 gatagcttac attattttat tatggaaaat aatagtaaat ggtacaaaat ttgattttta
      121 agatttaaaa cttttttaaa aagtgctaca atagaccaaa ttattacttt ctatactcgt
      181 taaacaggag gtgggactat gaaaaaacat aatattttat ttgtatcaac tgatgacaaa
      241 ataaatatag atatatcaaa acaattggaa aatatatttg gagaattttg taatatagat
      301 aacttggtat atgtaaatag gataaatatt gaactgtcaa gctatgagct tgtagtatgt
      361 tcagacaatg atataaagga atatatacat aataatattg ataaaaatat acttattgta
      421 atagtacatc gtactataaa tattgaaaat ataaatcaga taataagtat agaaaatgat
      481 tctgatgtta tggttataga tgcttataaa gagtcagcag atgaaactgc caagataatt
      541 agaaaactag gattaattca tattaatcta ataccatatt atccaggttg tgataagtct
      601 aaatgtgaga ttggaattat tacgggaagc agaaacagca taccacaaaa cataaagcaa
      661 ataattgaca taggtgataa ggtaattgat ataaatacag ttatagaaat ctttacaaaa
      721 ctaaatatat ctatagataa attacatatt attaaggaaa aatacaatga agatacagta
      781 agtggatata ggtattatac aactatgaat aaaaccatga agagtttttt ggagattata
      841 gatgaaggta ttgcctctgt tgataaatta ggaaaattta tatattgcaa caaagtattt
      901 tctaatctag ttggaataga ccaaaatgaa attatatcta ataactttat ggacttattt
      961 agtgataagg ttgtcaaaaa aatatttttt caagaagatg aagttaatga tgaagtagta
     1021 aatttaaata acaaaaaact gataattaat aaagtaaatg tttatgaaaa taatgaacga
     1081 ataaagagta ttataagcat taaaaatata agtgcaattc aagtgcttga ggacaagatt
     1141 caaaataaac ttcagtcaaa aggttttgta tcaaagtata catttgagag tgttgtaggt
     1201 gagagtaaaa taataaaaga aaaaattaat attgcaagaa aaatagcaac tacagacttt
     1261 tctgtgttga tacttggaga aaatggaaca ggaaaagaaa tatttgccca ggcaatacat
     1321 aatgaatctc taagaaaaaa caagccattt gttgcagtta atctatcttc tttgtcagat
     1381 acacttattg aaagtgaact ttttggctat gatgaaggaa gttttacagg agcaataaaa
     1441 ggtggaaaaa tgggtatatt tgaaagagcg catacaggaa caattttttt agatgagata
     1501 ggtgatatat ctttagatgt acagcagaga ttgctaagag tattacagga aaaagaagtt
     1561 atgagaatgg gcggaagtaa aattatacca atagatgtta gaattatagc tgcaacaaat
     1621 aaagatttaa agaaaaaaat taaagaaggt tcttttagag aagatttgta ttataggatt
     1681 aatgtattac atatagagat acctagactt agagaacgta aggaagatat acctcttata
     1741 agtaagtatt ttttagatga aataaatagc aacaaatgtt ttacagagga atcaatgcaa
     1801 gctttaacat tgtatgaatg gccaggaaat gtaagagagc ttaaaaattt aatttattat
     1861 attgatacta tagttgagga agatagagta gattacgagc atttgccaga acaatttaga
     1921 tttgaaaaaa acaatacttt agttaatgaa aatttcgatt caattatctt agatttcaaa
     1981 caatctaatt tctttgagga aagtatatgt atattgaaca gtatagaaac ttggaataat
     2041 aaaaatatat tattaggaag aaataaactg caagagattt taaaagaaaa agatatagtt
     2101 ttaagtgtag accaaattag aaaaagaata gataagttaa agagccatgg tttactttta
     2161 tctggagtaa aaaaacaagg tagttttata actgatgaag gaaagaactt tatatcatat
     2221 gtaaaaatta agggagtgat ataggggact aaaaaggcaa aacaccctaa aaaaatccct
     2281 ataatggata gaaataaata aaattatctt taagattaag ataattcaaa aatattgaat
     2341 aaataatcta agaagattat ttttaataaa acagtagtaa atatcaacct tactactgtt
     2401 tttatttttt gcaagattta ttatacaatt aaaatttata ttttggcata tcaattgcta
     2461 cataggaaaa atataaaaaa ttgtagaact tggaggatgt tgttatgaag tcagcttaca
     2521 aaggatatga agcctatagt tatttagaaa aaggaaaaga tatttcatct attgagatgt
     2581 gtaaaggaga taaaagagtt aaagagtacc ttattgaatt agatgatatt caagaagcta
     2641 aggttaaaag tatagtggat gaaaaaatat ttatatcatt acatgaacat ccagtattat
     2701 ttccagaaca cctagaaaga gatatatttg aatataatag ggaaggtaaa caaagatgcg
     2761 cttatgaagc attatcaaga ggatattatg atgctatatt tgataattta atggatggtg
     2821 tatgtacgat aacatcaaat tcagggtgga aatgggatga tatattaata gatttaggca
     2881 tgagactatg tgatttagct catcaagatt ttgtcataaa gtgtgaaaaa gtagaagata
     2941 tatgcagagc taaaagtgaa ggtaaagtag cattgatacc aacattagaa ggtgcagcac
     3001 caatagaaaa tgaacttgat agaatagata tactgtatgg atttggtata aggttaatgg
     3061 gtataacata cagcgaatcc aatgcattag gttcaggact aaaagaagag aaagatggag
     3121 gtcttacaaa tttcggaaaa aaagctgttg aaagaatgaa taaaattgga atggcaatag
     3181 attgctctca cgttggagtt caaactacac ttgatgtaat agaatatagc aaggacccaa
     3241 taatattaag ccatgttggg gcaagaaatt tatggaacac aaagagacta actccagatg
     3301 aagtattaat agcatgtgct aaaaaaggag gggttatagg tgtagaagca gcacctcata
     3361 ctacaataac agagaagaat aaagaacatt caatagagtc atttatggaa cattttgagt
     3421 atattaaaaa tttagtagga atagaccatg tagcttttgg accagataca gtatatggcg
     3481 accatgtaaa attacactca gtactagctg ctaaatttga tataaaaaaa ataacaggta
     3541 caaaagaaca tcccaaagta gaatatgtaa aaggtcttga gaacccaaca gaaacttcaa
     3601 aaaacattgt cagatattta gtaaagcatg gatatagtga tgaggaaata gagaaggtac
     3661 taggtggaaa tatacttaga gtactaaaac aggtttggaa atagatatat tagaaatatt
     3721 aataaaattt gaggatttat agggaggttt tataaatgga ttatttaaaa ttagctaatc
     3781 atccactttt gtggatagcg agtacagtag cagtatcaat agtagtggta caatccctta
     3841 tatttgctat aaaatcttgc aaagttggta aaacaatggg aataacagat aaacaaataa
     3901 agtcagctgt taagagtagt gctatttcag ctattgggcc ttcaatgact atatttgctg
     3961 gaatggtatc gcttatagta actatgggtg ggcctatagc atggatgaga ttatcattta
     4021 taggttctgt aatttttgaa tcaatgtcag ctggatttgg aacagatgca ttaggtataa
     4081 ctttaggttc tcctgaaatg acaaaacttg catttacaaa tgcagtttgg actatgatat
     4141 taggttcttt aggatggata atattcacat tactatttgg acataaacta gataagataa
     4201 ccaatgttat atctagtggc aagaaatcat ttataccaat aatatctcta ggagcaatgt
     4261 tgggttcatt tgcatatcta aatgctgata gggtgttgcg ttttgataat ggaacaattg
     4321 catgtataag tggaatggct ataatggtaa tattgtgtat gcttgaaaag aagaaaaata
     4381 taaaatggtt aagagagtgg gggcttacaa tatcaatgtt tagcggaatg ataatagctt
     4441 cagtaatata aggggggagt tttatgagta aagtaaaatc tatatacaat gaagaatatt
     4501 taccttttat gataaggtat ggaagactaa cactatcttt aggtattata gcagcattag
     4561 ttcctggaat aattttgagc tttggatttg gtattatgcc tccaatatca gcattattag
     4621 caagtacaat ggcaattgtc agtatgagtg ctccaaatta cataatagaa ccagtttctt
     4681 attcaccaat cttaggtatc ccaggaactt atatgagttt tctttctggt aatatatcta
     4741 atatgaggct accatgttca atagcagctc aaaaggcagc agaagttgaa tcaggtacag
     4801 aagaaggctc gataatatct acaataggaa tagctgtatc tattttagtg aatataagta
     4861 tactaactat aggggtaatt ttaggaggaa gcgtattatc taaaattcca gctgaagtag
     4921 tagaaaaatt aaacttaatt ttaccagctc tatttgggtc agtttttgga caagtatttt
     4981 tacaagataa aaaattaggt ctagtagcaa ttgtaatatc tgtattgact attatattat
     5041 caaagcaagg tataatacca cagtctctag tagttcttat atgtgtattt ggtactatat
     5101 taattgcaag agctatgtat aaagataagc ttagtgacta attacaatga aataatagtg
     5161 aattttcaaa tatgaaatat aataatttga ttttcaaata taaaaattac attaagatta
     5221 tttaaaaatt ggtttttaag gagggaatat tttgatatat gatttaatct taaaaaatgg
     5281 atttataata gatggtacag gtaatcctgg tttttatgga gatatagcaa ttaaagacaa
     5341 cttaatagca aaaatagatt ctaagataaa tagcaataca aataaagaga ttgattgttg
     5401 tggaaaagtt ataacacctg gatttattga ccctcatgtt catgaagaaa tagtagcaat
     5461 actagatggt aaatttgaaa agtttctaaa acaaggagtt acaacaacta taaatggtaa
     5521 ttgtggacat tctataacac catattcttc tgaaaatgta tatgaatata tgtataaaaa
     5581 tggtctatta cttgaagaag aaaaaaagta tcttatagat aaaaataaat gttggaataa
     5641 ttttacagaa tattgtgacc ttatatctaa aagtggtatt tctgtcaata tgggattctt
     5701 gttggggcat gggactataa gatggagtgt tatgggaggt tctaaggata gacctcctac
     5761 agaaaaggaa aaaaatgaaa ttacagatat aataaatgat ggaatgaaaa gtggtgcttt
     5821 tggaatatct actggattgg catatatacc aagtaagtat gctgatattg atgaacttgt
     5881 tgatattgca aggcagatta aagagcatga tggtatatat acatctcata ttagagatta
     5941 cataggaaga tataatgcag taaaagaagc catagaggtt gggcaaaaaa gtggtgctag
     6001 ggttcaagta tctcacttaa gtccagttga aatagaagca tttgatgaga tattaaaggc
     6061 taggtataat ggtgttgaaa taatggttga cacagtacca agaagttctg ggcattgtat
     6121 gaagaaaaaa agagtaattc aatttattat ggcaatatca tctagtctat ttgaattagg
     6181 tatagatggg gtaatggatg atcttagaaa tgaagaggga agaacactta tattaaaaga
     6241 agcatttata cttggagata gggggagtat aatacttcta aacactaaag atattaatat
     6301 ggaaaagaaa tcaatacgtg aaatagctac acaaaaagga atagatgagg acaagttatt
     6361 actcgattta cttttagatg gagacgaaga acttatcttt tgtttaggag gaatgtatag
     6421 agctgatttc ccagataaac tacatgataa taaaataatt gataatccat ttgttatggt
     6481 tggttcagat tgtttgttta gtgtgggtgg ggatatgtca tggtttgaac tacaaagaag
     6541 aggagctttt ccaattttct tcaatatgta cagaaagtct ggtgttagat tggaagagat
     6601 tgtaagaaga gttacttcat taccagctag gcaatttaaa ataaaaaata gaggtattat
     6661 taaagaaggg ttgatagctg atatagcagt tattgacatg gataactata gttattctag
     6721 gtcagaagat attgattttt caaagccaca attactagcc aatggtgttg aatatgtaat
     6781 tgtaaatgga aaaattgcgt tagaaaatgg agagattact gagaataaat gtggtgaagt
     6841 attaaaaaga taaggtttta aaaataatag gatttagaaa gtaaataaat atctaagtaa
     6901 gtaaagacga agtttgggat ttatttaaat aaaaatcatg cttcg
//
LOCUS       DAEPOG010000184         6896 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEPOG010000184 DAEPOG010000000
VERSION     DAEPOG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.184"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            87..362
                     /locus_tag="KR432_003172"
     CDS             87..362
                     /locus_tag="KR432_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641683.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            618..2318
                     /locus_tag="KR432_003173"
     CDS             618..2318
                     /locus_tag="KR432_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641684.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2681..3868
                     /locus_tag="KR432_003174"
     CDS             2681..3868
                     /locus_tag="KR432_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641685.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            3928..5091
                     /locus_tag="KR432_003175"
     CDS             3928..5091
                     /locus_tag="KR432_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641686.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            5242..6045
                     /gene="zupT"
                     /locus_tag="KR432_003176"
     CDS             5242..6045
                     /gene="zupT"
                     /locus_tag="KR432_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH0641687.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            6141..6701
                     /locus_tag="KR432_003177"
     CDS             6141..6701
                     /locus_tag="KR432_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBH0641688.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
BASE COUNT         2580 a          738 c         1268 g         2310 t
ORIGIN      
        1 aattatttat ataaataaag atataagaaa tttatcaatt ttattttgag ttataaaata
       61 ttaaagaaat tgtaggagga caatatatga aaaaaatatt agtagcatgt ggagcaggaa
      121 tcgcaacatc aacaatagtt tgtgacagag tagaaagatt ggttaaagaa aataatatac
      181 acgcagaagt tgagcaatgt aaaattgctg aatgttcaac aaaacaagaa ggtgctgatt
      241 taatcgtatc tactactata ctaccaacaa cttatgatat acctacaata aaggcaacag
      301 gatatataac aggtataaat acaacagcat tagataaaaa gatattaaat gcattgaagt
      361 agttttgtgt ttataaaaaa aatttagtat taaatataga gttgtatctt tataaaattt
      421 taaatataga gatatagctc tttttatgct gtttcaagta tacaactata tgatataaat
      481 cttcaatttt atgaaaaaga ttgttattac tttgtaagtt tataaaagat ttaaagttat
      541 tgttatgtta tactaattta tgggtatttt ttagaagtac tatatagaag tactaataaa
      601 ggagggattt aatacttatg ataaaacaat ttgtcaaata ttataagcca tataagaaaa
      661 tttttacact agaccttata gcagcttttc tattttcctt atgtgattta gtttatccta
      721 tgataactag aaatataatg gatgacgttg ttccaaataa aaacttaaga atgttagtag
      781 tttttgcagt agcacttata cttatattta tagcaaaagc agggcttaac tattttatgc
      841 agtattgggg acatgttatt ggtgtagata tgcaagctga tatgagaaat gaggtattta
      901 cacacttaca aagacttcca aatacgtatt ttgataataa caaatctgga gtaactatgt
      961 ctagaatagt aaatgattta atggatataa cagaacttgc acatcatggt cctgaagatt
     1021 tattcatttc tatagtaatg ttggttggtt cattctttat acttatagat ataaatattc
     1081 ctcttacgct tataatattt gctatattac cgtttataat ttggtttgca atagctaaaa
     1141 aagataagat gaatattgct tttatgaaga gtagagttac tataggtgat gtaaatgcta
     1201 ctttagaaaa tagtatagca ggtatgaaag taactaagtc tttttgtact gagaaagaag
     1261 agttaaataa gtttgtaaga agcaataaat tatttagaag agcaagacaa gattcttata
     1321 aggtaatggc agaatattac tctggtatga atttatacat ggatatctta gagtgggtag
     1381 ttgtaatagc tggtggttat tttacttata taggaaaaat tactttgggt gattttgctg
     1441 cttatatact atatgtaaaa atgtttatac aacctatgaa aaaattaata aattttacag
     1501 agcaatatca aaatggtatg acaggtttta aaagattcat agagataatg gaacaagacc
     1561 atcaaaaaga agctaaaaat cctattgaac ttgaaaatgt aaaaggtgac attgagatag
     1621 aaaatatatc tttcacatat gaagataaga ctcaagtttt ggataattta agtttatcta
     1681 taaaagctgg taagactata gcattagttg gaccttctgg aggaggaaag actacattat
     1741 gtaatctatt gccaagattt tatgaatttg ataagggtga tataaaaatt gatggaaaga
     1801 gtataaaaga tgttagttta aaatcattga gaaaaaatat aggtatagtt caacaagatg
     1861 tatttttatt tacaggaact ataagagata atattctttg tgggaaccct aatgctacag
     1921 atgaagaaat gatagctgct gctaaaaaag ctagaataca tgactttgta gagactttgc
     1981 cagatggata tgatacttat ataggagaaa gaggcgtaaa attatcaggt gggcaaaaac
     2041 aaagaatttc aatttcaaga atctttttaa agaatcctcc aataataata ttggatgagg
     2101 ctacatctgc tttagacaat gtaacagaaa gagaaattca agagtcttta gaggaattaa
     2161 gtaaagatag aacaaattta gttgttgcac atagattgac tactataaaa aatgctgatg
     2221 aaatcatagt attgacggat aaaggaattg aagaaagagg tactcatgaa gagttagtaa
     2281 acaaaaatgg agtatatagt aggttacaca ataactagta tacaaatttt acaccatagc
     2341 taacatacgc attaaactta caaacatagg atatattaag attcataaag acaattcatt
     2401 ttatatttat gagatgtttt atgaataaaa tgtatcctat gttttatttt ttgcaaatta
     2461 catttaattc tctttgtaag aatagagtta aatagccaat ggtcaggata acgtttttac
     2521 aaaaagaata atttagaaaa ctaactttaa tttatggaat ttatcgaatt ttatgaaaaa
     2581 aatcgaattt tgacttgaaa tgtttttcaa aagtagtaaa ataatggata actatacata
     2641 cattttataa aaatacattt attatgaggg gggattttgt atggaggcaa taacagcttt
     2701 ttgtctagtt ttaatatcac tgacgatagg agatatagtt tcagcaaaaa ctaaagcttt
     2761 tgttccgtcg gtttttgtat cagcaattat atttatagta ggattctgga cttttttccc
     2821 agaaaatatt gtggacttag ccgctttagg aacaccatta gctcaattag gtatgctttt
     2881 gttaataact catatgggaa ctatgatgag tattaaggaa ttagcaggtc aatggaaaac
     2941 aatagtaata gccttggcag gaatagtagg aatatgtgta ggtgctttgg cattagggac
     3001 aatagtattt ggatgggata cagcagtaat agctacacct cctcttactg gagggctagt
     3061 tgcatctata atgatggcag atgctgctac agcaaaaggt cttgcaagtt tatcagtact
     3121 tgcaattttg atgtatgttg cacaaggatt tgcgggttat cctattacag cattaatgct
     3181 taaaaaagaa ggaaaaagac ttttatcaga ctttagaagt ggaaaagtaa ctgtaaatac
     3241 agatgaagaa aaagttaagg atttacctga acaaaaaagc agatttaaga taattccaga
     3301 cttaccagaa aaatatgata cgacatatat gataatactt agattaggat ttgtagcatg
     3361 gttagcagta caatttacaa atcttacaaa tgaagttgta tcaagatatg ttatatgttt
     3421 aatatttggt gtaatagcat cagaacttgg aatagttgat agaaaaccac ttaataaatc
     3481 tggttcattt ggatggttaa tgacatcatt gatggctttt atatttgcag gattagcaaa
     3541 agctacacca gcaatgcttg ttgaaatggc agttcctctt gtaggaatca taatatttgg
     3601 tgtaataggt atgggaatat tctctattat agtaggtaag cttttaggtg aaagtaaaga
     3661 aatggctttt tcagtttcgc ttacatcact ttatggtttt ccaccaaact atatattaac
     3721 tgaagaggca gtaaaggctt tggctgaaac accagaagaa aaagaatttt taatggataa
     3781 gatgcttcca aagatgcttg ttggaggatt tactacagtt acaatagctt ctgtagtaat
     3841 agcaggtatc tttgtaaatc taatatagta tttaagtgat aaaaagtatt ttaaataaga
     3901 agaatgtgta ctacttggag gtatggaatg gatattaaag aaataacaaa gagttataaa
     3961 gactatgtaa ttaagcttag aagggaattt catgaaaatc ctgagaaaag tatggaagaa
     4021 gttagaacat caaagagagt taaagaagaa cttgataaaa tagagatacc atatgtatca
     4081 gctggaggta caggtgtaat tgcaactata aaaggagcta atccaggaaa gactgtagca
     4141 cttaggggtg atatggatgc tctacaagtt gttgaatgca ctgatgtaga atacaaatct
     4201 aaaaatgaag gacttatgca tgcttgtggt catgatggtc acacatcaat gcttctaggg
     4261 gcagcaaaag tgttaaatga tattaaggat agtatcaatg gaactgtaaa attatttttt
     4321 cagccaggag aagaagttgg taaaggtgca agagctatga ttcaagatgg tgctatggaa
     4381 ggtgtagata gtgtgtttgg aatacatctt tggactgatg tagaatctgg gactatatcg
     4441 gtagaagaag gaccaagaat ggcttctgcc gatttcttta aaattactgt aaaaggtaga
     4501 ggtggtcatg gttcacttcc tcatcaaggt gttgatgcag tgcttgcaag ttcagcaata
     4561 gttatgaatc tacaatctat ggttagtaga gaagtaagtc ctctggaacc attagttgtg
     4621 agtgttggag ttttaaattc tggaactaga tttaacgtaa ttgcgagtga agctgtctta
     4681 gaaggtacaa taaggctatt taatccagag cttagaaagc aaattcctgg aattttagaa
     4741 cgtatagcaa aatctacagc agaagcatat agagcagatg ctgaattaga gtatgggtac
     4801 ttaacaccag cagttataaa tgacaaagaa tgttcaaaga tagctacaga tgcagcaatt
     4861 aagctatttg gagaagactg tataacacta tttgaaaaag taacaggagc agaagattta
     4921 gctgagttta tgaatatagc acctggtgca ctagcatttg taggagctag aaatgagtca
     4981 aaaggtgctt gctatcctca tcatcatgga tgctttaata tagacgaaga tgctctagaa
     5041 attggaactg ctttatatgt gcaatatgca gtggatttct taaataaata attttaatat
     5101 actataaaga aaaagcagag actaagttta tatctctgct tttttattgt gtatattttt
     5161 tataaatatt gactatataa aatatatatg atagcataaa gaaaatgata atcaatatca
     5221 attaaaaaga aggtgattta tttgaataat gtaatatttg catttttgat aacattattg
     5281 gctggtttat ctactggtat aggaagttgt atagcatttt ttgctaaaaa aacaaatact
     5341 aagtttcttt ctataagctt aggattttct gctggtgtta tgatttatgt ttctatgata
     5401 gaaatttttc ctaaagcaca agatgctttg acaaaatcta tgggagagaa attaggaagt
     5461 tggtcaactg taatagcttt ttttataggt atggctatca ttgctgttat tgataagtta
     5521 attccacaag aagaaaatcc acatgaaata aagaaaatgg agaatataga agaaaagaat
     5581 atcaaaaaaa ataagtctct tttgagaaca ggtatattta cagcaatggc tatagcaatt
     5641 cacaattttc ctgaaggatt agctacattt atatcagctc ttgatgatgt tacaatagct
     5701 atcccgatag caattgcaat tgcaatacac aatataccag aaggtatatc tgtatctgta
     5761 ccagtttatt atgccacagg agataaaaag aaagcttttt attattcctt tttatctggt
     5821 atgtctgagc ctttaggagc gattattgga tatgtacttc ttagaaattt tttaaatgat
     5881 ataactcttg gaattgtatt tgcaattgta ggaggaatta tggtattcat atcactagat
     5941 gaattattgc catcagctag agagtacggg gaacatcatt tatctattta tggattgatt
     6001 gcaggaatgg gtgttatggc tattagttta ttactattta aatagctttt taaatatatg
     6061 attgtaacct aaaattagta tcaaaatgat ataattattt aaatagattt ttaagctatt
     6121 taaaaatatc attttggaga atgagtgcta tggaaaagaa aataaaattc aaatttgact
     6181 ggtatagttc acctgataag atagagaaat ggttagaaga tattgaagct aaaggatata
     6241 atttatataa gataaataaa tttggaggaa tattttattt tcataaggga agtccaagaa
     6301 aaataaaata cactgttgat tactactatg atgtagagaa taattatttt gatgaacata
     6361 aatcaaatgg ctggaatctt gtatttagga gtattggtag ttttatgggt acatctgcat
     6421 ggtatatatg gtcgaaagaa tacaaagaca tacctcctaa attatatagt gattctgata
     6481 aaaaaaacaa agtaaagtca attttattta tgaatatgtt tatattcttt atgttagtag
     6541 taatatttat atatttattg aatattgaaa taagaaattt atatagtggt tatggagcat
     6601 ctgtaactgc tattgtacta cagattatat tgttgatttt atatgcagta caattatata
     6661 ggagattaat gtattattta aggcttaaaa agtttaaata aataaaagaa ggtgtcctaa
     6721 aattattttt ttaatatttt aagacatctt cttttgttag taaagtactg tttttaatta
     6781 tagtaatttc tgtctttaaa ttgaaatatc aaataatagt tgtattggat tgagtattga
     6841 taaaaataat ttaaaaatgt tatcctaaag tatcagatat acaaaattat ctttaa
//
LOCUS       DAEPOG010000185         6847 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEPOG010000185 DAEPOG010000000
VERSION     DAEPOG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.185"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            194..1015
                     /gene="yidA"
                     /locus_tag="KR432_003178"
     CDS             194..1015
                     /gene="yidA"
                     /locus_tag="KR432_003178"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH0641689.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1151..1300)
                     /locus_tag="KR432_003179"
     CDS             complement(1151..1300)
                     /locus_tag="KR432_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641690.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1307..1792)
                     /locus_tag="KR432_003180"
     CDS             complement(1307..1792)
                     /locus_tag="KR432_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641691.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1805..2557)
                     /locus_tag="KR432_003181"
     CDS             complement(1805..2557)
                     /locus_tag="KR432_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641692.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2557..2694)
                     /locus_tag="KR432_003182"
     CDS             complement(2557..2694)
                     /locus_tag="KR432_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0641693.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2734..3363)
                     /locus_tag="KR432_003183"
     CDS             complement(2734..3363)
                     /locus_tag="KR432_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641694.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3324..3887)
                     /locus_tag="KR432_003184"
     CDS             complement(3324..3887)
                     /locus_tag="KR432_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641695.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(3897..4121)
                     /locus_tag="KR432_003185"
     CDS             complement(3897..4121)
                     /locus_tag="KR432_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641696.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4126..5217)
                     /locus_tag="KR432_003186"
     CDS             complement(4126..5217)
                     /locus_tag="KR432_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0641697.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5252..5710)
                     /locus_tag="KR432_003187"
     CDS             complement(5252..5710)
                     /locus_tag="KR432_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641698.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5703..6788)
                     /locus_tag="KR432_003188"
     CDS             complement(5703..6788)
                     /locus_tag="KR432_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0641699.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
BASE COUNT         2245 a         1214 c          777 g         2611 t
ORIGIN      
        1 aaagttttta cattctcatc tcacataagt aatattttta tcaaaaatta atattattaa
       61 actttctaaa ttacttatct cttgatataa tagtatacaa aacaaaccat atttcccact
      121 gaaactttgt tcaacactag ctgtgtcaac gttttcacag gaaatttgag tatataattt
      181 attggaggtt gatatgtaca aattaatcgc actagacatt gatggaacaa tattaaatac
      241 acaaaaaagg attactcctg aagtctttga gtccattcag gaggcaaaaa gggctggggc
      301 aaaagtagtt ataacaacag gcagacctct tcctggtgta aaagaacttc taaatcaact
      361 aaatttaaca gatgaaggtg attatgttat atgctttaat ggagcaatta ttcaagaagt
      421 taaaagtgag aaaatcatac atgatgtaga aatgacttta gatgattttg attttatata
      481 taataacgtt tgtaaaaaat ataagactaa aatacacata aatacaatga caaatttaat
      541 tacaccaaat gaagcacctg gaaaatatac acttcatgaa gcgaaattaa ataatataga
      601 agtcaaatat atacaaaaag ataaaattga tgaatctatt aaaatctgta aaattatgat
      661 agttgatgaa ccagaaagat tggaagaaat catacagcaa ctacctaaaa atttattcaa
      721 taaatatact atagtaaggt ctgctccttt ttatctagaa tttttaggta aaactactaa
      781 taaaggaact gctcttaaaa cattatgtac gaatctaaat atacctatag aaaatgctat
      841 tgctgttggc gatgaagaaa atgaccaaca tatgataaag tatgcaggtc ttggtgtggc
      901 tatggggaat gcacgtaata gtataaagga aattgcagat tatgttactg atactaataa
      961 tgaaaatgga gttgcaaagg ttataaataa atatatactt aataaggcta tctaatcatc
     1021 taaaaaaact aaaaatatac ctttagattt aaatactttt cacatcccta ctctatacta
     1081 atttatatag tatggatatt acctatcatt tttatctaaa ggtcattttt agtttttcat
     1141 atatgtatat ctatgatagt accttttgtt tgagtttaaa tattttatat gattctacta
     1201 ttgcctcatt aatatctaaa caatatatat cttcatcatc agataatccc gcttcgtttg
     1261 tccaagcatc aaaatcactc ttcgtccaga agaaattcat tatatttcag caactagcag
     1321 cccaatttag atgcttatca agatttatat gaagcactct taagtttgga ttattcacat
     1381 actcaatctt ttcatttttt ataattactt ctatttcctt acctgtagca ctacaaatag
     1441 agtttatttt tatatcttgg ttgaatgtgc aagaggttcc tatagcatct atagcacaca
     1501 ttgcgctgaa acttctatta tctctcaaag ttactttatg atttgtttgc aatgctgata
     1561 caggatatat aaaatttata ctattcccat caacaaccat tatattttca taaataaaat
     1621 attgtagtgt cctttctata tattcttttt ctatattcaa ctttttactt acatattcag
     1681 tcacttcttg aagtgtaaca ctagaacctt tatctattat catatccata atagagattc
     1741 ttattttctg ttgtgtatta tccaaactat tgtatttttc tatttgactc atgtctactc
     1801 ctctttaaac ttcataacac tttgataaag tatcagttaa tttgtttttg tactcttcat
     1861 atttttggct tcctctaatt ctaggtcttg gaatatctat atttatatca tcaataattc
     1921 ttcctttatc tctcgacatt actataactc tatcactcat atatacagct tcatcaacat
     1981 catgagttac cattaatgca gttattttct tctttatcca tattcgttct agctcttcct
     2041 gtaaattttg tctcatttga aaatctacag ctcctaatgg ttcatccatt agtaatacct
     2101 ctggattaca tgctaaagct cttatcatgg ccactctttg tttcattcct ccagataatt
     2161 gatgcggaaa cagtttttct tttcctttca agtctgacat aaccaaaagt tcttctagca
     2221 tttcttctct tttttctttt ggtattttct gctgtttcat aggaaataga acattacctc
     2281 ttatagtttt ccaaggaaat aaagcataat tttgaaatat aaaagctcta tctatgcccg
     2341 gttttttaac tcttttatca tttataaaaa catctccacg ttctgatttt tgaaacccac
     2401 ctattatagt aagtaaagtt gtttttccac aacctgatgg tcctaaaaga cagacaaatt
     2461 gtccatcatc tatatccata cttatatctt ctaaaacctt tgtatgtact ctattattta
     2521 caaatgtctt atttatatct cttacactta acttcattaa tctgcaaacc tccatctaca
     2581 aaggatttta tttataaact ttaataccct atctattata aatcctatta aagctgcaaa
     2641 tatcataagc ccaattaata catctgtatg cattaatgtt tgagcttgta ccattgagta
     2701 accgagaccg gcactcgtag ctataaactc ggctcagata acagacatcc atcctgtact
     2761 tatagcaatc cttgaaccag ttagtatatc tggaagagat gcgggaacaa ttacatttgt
     2821 aaatatcaca attggcgatg ctcccatact tctagctgca ttataataat cctttgatat
     2881 tgctcttact ccttgaatag tatttaaaat tatagggaaa acccctgaaa aagctattaa
     2941 aaatattgtt ggaccatctc ctataccaaa ccatactata gtaagaggaa cccatgccat
     3001 tattggaact tgccttacag aatctactac tggagataaa accctttcaa aagttttaga
     3061 aaatcccata ataaatccta ttggaagccc aaacaataat gcatacataa aacctttaag
     3121 tactctctgc aatgttataa aaagattagt gactgtctct gcatctgtca cacagaaaaa
     3181 gaaacctttt attacatcta ctggcattgg aagtaacaaa gaactcccta tatcttttgc
     3241 tgcaaaatgc cagactaaaa atataataac taatagtatt attttatata aaatatccat
     3301 actaccatca gtttttttct tagttaatct atcgctttta tctttagaaa ttttaatttc
     3361 caattgctta tttcctccat atctatattt cttatattcc tgtactcttt taatgctttc
     3421 ttaaatgtag ctttattctt taaagcatca acacttttat tgtaaagtgt tataaaatca
     3481 ctatatgctt tacttttttt aaactcttta cttacaacta atacataggt atcataatct
     3541 cctaatttag ttgtagattt tcttttaccc tctaacccaa ttgactttat gcaatctatt
     3601 acaacaccat ctactaaatt actttctaat ccatatgcta gtgcagtccc taataatgga
     3661 gctacttcca cattcttgta gtatttttta accaattctg actggtaatc tctattttga
     3721 atgactccta tcttttcagg attattatca cttaatagaa atatatcaga attttgaact
     3781 acagtaccaa caatttcaaa tccactattt acatttacat attgttttgc agcatcttta
     3841 catatgattg ctatatctat ttcctctgaa ctcagagccc actgcattgt acttgctcag
     3901 caatcattaa ttgtaaaaat atccattata tcacttactt ttatattttc aaagtagtct
     3961 ctatttacca tataatttat tacaaaacca gaggtatcat cagatacccc tattcttaat
     4021 tttcctttta tttcttcttt atcttcaaaa ttaaaaaata ttataaatat acttcccaaa
     4081 ataatgataa aaagtattat tatatttttt atattctcca tttatttata atacctttgc
     4141 tttccagtct tcataactca taccttcagg aaactctggg ttaacttttt cttttataaa
     4201 tttgtcaaaa tcatcagctc cacaagagtc caataagctt gtatcaatcg tatcttcata
     4261 tttaggtata tcatctaaag cctttaaatc tttatacatt tgtaaattat ttttatattc
     4321 ttcacctgta actttccaag taagagttct accttctcca acagtcttct tatagatagt
     4381 tctaagtgca acttcttctt ctacattata atattttgca aatattttag ctgcttcagc
     4441 tggatttgta tatatatatt ctattgcttt tgtatgtgct agaaccaatt tttttgctaa
     4501 atctggatgt tcttttataa agtttttgtt taatgagaat gaacaacagt tatattcttt
     4561 gccattagct ttttctttat attgagtaga tgctattatt tttcctgcac cgtcattttc
     4621 agctacagaa ccccaagggt cacaagatgt aaaagcttga attttcccag ttttaagagc
     4681 taaatattta tctttgtctg aatccatatt tacaacttca tattttgatg ggtcagttgg
     4741 tagacctgtt tctggaccat aatcacttgt ccataagaaa tcactcatat ctgttgcaat
     4801 ttttttacct attaaatctt taggttcttt tatatctttt gaaactatta aatattcaga
     4861 accaccagta tggttgttag ctgctattgt tattggtgaa cccttaggta ttgctccaac
     4921 taacccctta gttcctatgt aacctgcatc catttttcct gctgccatag cttcaggaac
     4981 tttaccattt ccaacagttt ttacattaag acctaaatct ttatatattc ctgccgattc
     5041 tgctactggt cccgcagtca tatggtcaca attataataa cctactgtta ttgtataatc
     5101 atcttttcca gcttttgctt cttctttttc ttttggttta ctactacatc ctgttaatat
     5161 agatgtaccc attacaccaa ataagattag tgcttgaatc ttcttagata atttcatata
     5221 aatttcccac ctttatgtat tatttttatt tttagcaaca tttgtccata tcttcgccaa
     5281 ataaatcaac aaatttttta ccctctactc tgtaaatagc atatcctaca ttaccatttt
     5341 tagaccctga tactattact tctattggtg ctttatcatc aatattttta aaggttatag
     5401 tttgattctc tataggtgct ggtattggct ttgttatata gtggctattt ttatcagaaa
     5461 ctacgactac catttcattt gaattattgc tttttttata tataactact aaatcttttt
     5521 tcccatcatt atttaaatct tcttcagcac aagttattat gtcttcttta taattttttc
     5581 taaaatattc taatgtttct ttattcacat ttggaataga agcatcttct ttgatgttat
     5641 catcaactat ttttgataaa atgattaaca caactactat acaggatatt ataactatct
     5701 ttttaagcat gatttcctat gacctctttc atctttggta ttgttgattt aaatgcaaat
     5761 aaaataatta aaatagtaca tacatactca aaactatcag gtatatttaa cattagttta
     5821 tttgccaact gtattgaaga atttgttgca tctaaattta ctgaattatt attcactaaa
     5881 agtaaatttg ttatatatcc aattattatt gaggacagtg tcaaagttac agaatatata
     5941 ataacagttc tttttccaat aagtttaaac atgcttataa gttctggtag atttgtagct
     6001 gctccagcta ttagaaatgt tatcgctata ccaggagatg ctccacttgc aacaagggct
     6061 gctataaacg gtatatgtcc tacagcacat acatacataa cacatgcaag tattgctata
     6121 cttccaagag atagcaaacc tggatttcct aaatattttt gaattatact atttggaaaa
     6181 atagttgtta taaatcctgc aaaaaccata cccattacta catatttact aatagtaagg
     6241 gctaattcac caaaagacca tttaagacca tcaatcatat tttccataaa acttcttttt
     6301 tcttcttcta attctattac ttcttcctct gaatcattat aaagtgataa ttcttctttt
     6361 ccaaaacaat ttcctaaaaa tcctactaaa aatggtacta aaaaaccagc tattagataa
     6421 atgattgtaa ctttaggacc taaaagacct agacaaagaa ctacagctat tggatttatt
     6481 attggagttg atgtcataaa agcaagtact ggtcctaaat atgctcctga ataatacata
     6541 cttattccaa gtggtattac tccacaacta catattggta aaaacattcc tgagatagta
     6601 cttttaatta aagaagaaaa tttcttattt ccaagttgtt tttgaaacct tcttggagtt
     6661 aaaacattgt gaagtacacc tgccaatata aaacttatca taagccatcc tgatgctcca
     6721 ttcagtgtaa ctatagaagc tactatagta tctttaactc catttaaaac aatttctaat
     6781 atattcattt ttgatactcc taatttttaa acttttaatt attgtttata gcttctacta
     6841 ttgccct
//
LOCUS       DAEPOG010000186         6838 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEPOG010000186 DAEPOG010000000
VERSION     DAEPOG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.186"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..383
                     /locus_tag="KR432_003189"
     CDS             <1..383
                     /locus_tag="KR432_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641700.1"
                     /translation="EQKLDAKVYKIYENNNKIMVKLKISNQIVANQDTSVEEFDIIYN
                     QMECLKIPKTSIKTKDNKRGVYVIDEQSQSVKFVILEGITYENESSVFVDYYKNDING
                     VTSVNLYDKIILRPNSINTNIRIK"
     gene            393..1097
                     /locus_tag="KR432_003190"
     CDS             393..1097
                     /locus_tag="KR432_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH0641701.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            1137..1595
                     /locus_tag="KR432_003191"
     CDS             1137..1595
                     /locus_tag="KR432_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH0641702.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            1614..1871
                     /locus_tag="KR432_003192"
     CDS             1614..1871
                     /locus_tag="KR432_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH0641703.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            1881..2666
                     /locus_tag="KR432_003193"
     CDS             1881..2666
                     /locus_tag="KR432_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH0641704.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            2701..3228
                     /locus_tag="KR432_003194"
     CDS             2701..3228
                     /locus_tag="KR432_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH0641705.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     misc_binding    3403..3635
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3728..6835
                     /locus_tag="KR432_003195"
     CDS             3728..6835
                     /locus_tag="KR432_003195"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH0641706.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
BASE COUNT         2798 a          712 c         1290 g         2038 t
ORIGIN      
        1 atgagcaaaa attagatgca aaagtttaca aaatatatga aaataataat aaaataatgg
       61 taaaattaaa aataagtaac caaattgttg caaatcagga tacaagtgtc gaagaatttg
      121 atataatata taatcagatg gaatgtttaa aaatacctaa aacttctata aaaacaaaag
      181 acaataaacg tggtgtatat gtaattgatg aacaatcaca aagtgtaaaa tttgtcatat
      241 tagaaggaat aacttatgag aatgaatctt ctgtatttgt ggattattat aaaaatgaca
      301 taaatggagt tacaagtgtt aatctatatg acaaaataat tttaagacct aacagtataa
      361 acacaaatat aaggataaag taggtgataa taatgggttc tattaaagat aatttgtctg
      421 agataagaaa caaaattgat aaagctgcaa aaaaagtaga tagagacagc aaagagatta
      481 cccttttggc agtcacaaag actgttgatg tagacttagt taatgaggca atgagggaag
      541 gaatcacttc tgttggtgaa aataaacccc aagaattagc tagaaagtat gatattatag
      601 gtgacaaatt aaactggcac ctgataggaa ctttgcaaac caataaagtt aagtacataa
      661 tcgataaagt ttgtatgata cactctttag atagaattgc attatgcgaa gaaattcaaa
      721 aacgtgcaga acaaataaac agagaaatag attgcttagt acaagtgaat atatctaaag
      781 aagagtctaa gcatggactt gatagagaag aagttgtaga ttttgtcaaa gaagtgtcag
      841 aaaaatataa aaatataaaa ataaagggtc taatgacaat ggcacctttt atagaagatg
      901 aagatgagat aagaggtgta tttaaaggtc ttaaagattt atcaaaacaa ataaaagaac
      961 taaacatccc taatgtaggg atggatgttt tgtcaatggg aatgagtcac gattatgaaa
     1021 ttgctataga ggaaggggct accattgtta gagttggaac atcaatcttt ggagaaagaa
     1081 actacaataa aaaataatta ctcaaatttg aataaatttt aaggaggaag ttaataatgt
     1141 caaatggaat aatatctaaa tttaagaatt ggatagttga tgaagaagaa gattatattg
     1201 aagatgaata tgaaagtggc atggacgata tagttcaaga agaagaaatg aacagtggct
     1261 ttagtactgc aaaagcaaat aaaattgtaa atttacatac aactagccaa atgaaagttg
     1321 taatagttga acctaaggtt tatgatgaag ctgcaactat agcagaccat ctaaaacaaa
     1381 gaagggcagt tatagtgaat ttggaaggat tgactaattc tgaagttcgt aaatctatat
     1441 ttaactttat gaatggggca gtttatgtat tagatggaag tatacaaaag gtttctaaat
     1501 caatatttat actagctcct aataatgtgg atatagatgc aaatatgaaa aaagaattag
     1561 aaagtaaagc attcttccct tggcaaaata aatagtaaaa aggcggttaa tatatgggaa
     1621 caataagaat tgcactttat tatcttttag atataatagc atggatgata gttataaaaa
     1681 gcttgatgac ttggtttcca aatggaagac aaagtaaaat ttttaatatt ttggaaaatc
     1741 ttacagagcc aatagaaggg ccaataaggt ctataatgta taaatataca aatggaccta
     1801 tagatttttc tcctttaatt gcaatagtat tattgatgtt cctaagacaa ttagtattgg
     1861 ttatattcta aggtgatagg atggataaat taaaactaac aaatcacata aaggatatag
     1921 acttgaaaaa taaaatgttt aaagtgatag acaaggctaa tagttgtata aaaaactatg
     1981 atgttaagtc tacggagttt cttaatccat atgaagtgaa aaatgctgta gcaatattaa
     2041 attctacaaa tgaaataaaa tacagtgtgg atggaggtta tgaacaagct gaaagaagca
     2101 ctgtatttat ttatccattt tacatggaat atgaagatat agaagatact cttagatttt
     2161 tacaaataga agggaatttc aagtttaaaa atatatctca taaagattac ttagggtcaa
     2221 tactgggtct aggtataaaa agagaaaaaa ttggagatat aattatacat gatagctttt
     2281 gtcaagtagt tgtaagtagt gatatttgcg attttataat tgtaaactta gaaaaggttt
     2341 caagaaataa tgtaattgta aaggaaattt ccagagaaaa tattgttaat agttcttcaa
     2401 aatataaaga agtatcattt acagtatctt cagataggtt agattgtgtg ataagtggta
     2461 tttacaatat atctagacaa gatagtgcaa aatacataaa tggagaaaaa gtccatgtca
     2521 attatgaaaa gataacttca acttctaaaa tagtaaaaaa tgatgatttg atttctatta
     2581 gaggcaaagg aagagctaag gtaacacaaa taggagatat taccaaaaaa ggaaaaataa
     2641 aagttcaagc aagattaatt gtataaggac taatttaaat tatagctggg aggaaatgta
     2701 atgctaactc caattgagat agaaaataaa gagtttaaaa aaggtttaag agggtataga
     2761 gatgaagagg ttgatgaatt tttagatatc atcaaggaag attatgaatc tctatgtagg
     2821 gaaaatacag ctcttaaaga aaaattaggg ttatatcaag accaagtaaa caagtatgaa
     2881 aatattgaag aaacactaaa agctacttta ataactgctc aaagtgcagc agaagacact
     2941 tgtagcgcgg ctaataaaaa ggctaagata attgttgaag aagctgattt aaaagctaga
     3001 caaattatag aacaagctaa taaccgtgtc atagagataa gaaaagagta tgatgcttta
     3061 gtcaaagaat ttaagatatt tagaaataaa tttaaatcac tgttagaaga tgaaataaga
     3121 agtgttgatg agatttttta tgatgtagat gaaaaatgtg taaatgcatt tgaaggaaca
     3181 acagtgtata attatagtaa tgatgaagct gctacaactt tagaataagc ttgtaatttg
     3241 tttatttttt atgtagaata actgtgtgag tttgagaaat ctataacaaa gattaaaaat
     3301 aggataaaaa tgtaagaaaa atagaaatta ttatattttt caaaaatata ttgactattc
     3361 aaattatttg ttataaaatt tcatataaac acagataaat ttttaaaagc gttgatagag
     3421 acatttatct ttagataatt ttacagagaa ttgggtatgc tgagaaccaa tgaatttata
     3481 aagataaaaa tcactcttga gctgcaggct gaacgattaa attctactaa gctgtgccgg
     3541 ttagtaccgt tatactatta gagtgttata tgtagctagt atttagttta catatagaat
     3601 tagggtggta acgcgattta taactcgtcc ctttggttta aagggggcga gttttttagt
     3661 atttaaattt atgtgaaaga ataaaaaatt ttggaattat aatatttagt aggaaagggt
     3721 gatttacatg gcgaaattta agccattggt agacagttct gtaaaacaag cagaagcaca
     3781 agtttttgat tattggaaag acataaatat acttgaaaaa actttagaaa aagggaaaga
     3841 tgacccaagt ttcgtattct atgaaggacc tccaacagct aatggaaatc ctggagtaca
     3901 ccatgtttta tctagaacgc ttaaagattc agtttgtaga tataaaacaa tgtctggtta
     3961 tcaagtaaag agaaaagctg gttgggatac acatggatta ccagttgaaa tacaagtaga
     4021 gaaagagtta ggtcttacta gcaagcaaca aatagaagaa tatggaatag ctgaatttaa
     4081 ccaaaaatgt agagagtcag ttttctcatt tgaaaaacaa tggagaataa tgactgagag
     4141 aatggcttat gaagttgatt tagataatcc atatataact ttagataata actatataga
     4201 gtctgtatgg tggatactaa acaaattcaa caaagaagga tatatatatg aagggcataa
     4261 aatacttcct tattgcccaa gatgtggaac tggtcttgct agtcatgagg ttgcacaagg
     4321 atataaagaa gtaaaaacta atacagttat agctaaattt aagaaaaaag atgctgatga
     4381 atacttctta gcatggacaa caactccatg gactttacca tcaaatgttt cattaacagt
     4441 aaatgctgat gtagattatt taaaagttaa aaaaggtgat gaggtatatt atgtatctaa
     4501 acctttagct gataaagtac taggtgaaga ttatgaagtt ttagaagaaa tgaaaggtaa
     4561 agacctagaa ggtttagaat atgaacaatt aatgcctttt gtagaagttg acaaaaaagc
     4621 attttttatt acagtaggag attatgtaac tactgaagat ggaactggta tagttcatac
     4681 agcaccagca tttggtgaag atgactacaa cacaggtaaa agatatgatt taccagttat
     4741 tcaaccagta gatgaaactg gtaaatttac atctactcct tgggaaggta gatttgtaat
     4801 ggaagatggg ctagatgttg aaataataaa atggctagct tctgaaaata aattatatag
     4861 caaagaaaaa gtagttcata attacccaca ctgctggaga tgtcaaactc cattagtata
     4921 ctatgcaaaa ccaagctggt atatagagat gactaaactt aaagataaat taatagaaaa
     4981 taataatggt gtaaaatggt tcccaagttt tgtaggagag aagagatttg gaaactggtt
     5041 agaaaatcta aatgactggg caatatcaag aactagatat tggggaactc cacttcctat
     5101 atggagatgt gaatgtggtc atacagattc agttggttca agagctgaat tagctgaaaa
     5161 agctatagaa gatgtaaatc cagaaacagt tgaacttcat agaccatatg tagatgatat
     5221 acacttaaaa tgtgaaaaat gtggaaaacc aatgacaaga gtaacagagg ttatagactg
     5281 ttggtttgat agtggggcta tgccatttgc tcaacaccac tatccatttg aaaataagga
     5341 aaattttgac caattgttcc cagctgacta tatatcagaa ggtatagacc agactagagg
     5401 ttggttctac tcattacttg cagtatcaac atttgtatta ggaaaagcac catataagag
     5461 tgttttagtt ccagaccttg tacttgataa agatggtaag aaaatgagta aatcaagagg
     5521 aaatacagta aatccaatgg aattgtttga ccaatatgga gcagatgcat taagatggta
     5581 tttactatat gtttctccac catggactcc aactaaattt gatatggatg gattaaaaga
     5641 aatccaaagt aaatttatag gtactatgaa gaatgtatat aacttcttta ctttatatgc
     5701 aaacacagat gatataaatc caactgaatt ttttgtagaa tataaagata gaccagaact
     5761 tgatagatgg atattatcta agttcaataa tttaatgaaa gatgtagaag aaaatttagc
     5821 tatatttgag ttaaataaaa ctttaaggat gatacaagat tttataaatg atgatttatc
     5881 taactggtat ataagacgtt caagaagaag attctgggca actgaattaa cagaagataa
     5941 aaaagcagtg tacaatacaa cttatgaatt gttacatggt ctttgtagag ctatagctcc
     6001 atttgcacca tatatgtcag aagaaatgta tagaaactta acaggagaag tatctgttca
     6061 tttagctgaa tatcctaaat gtaatgaaga gttagtagat actaaattag aagaaaaaat
     6121 ggatttagct aaaaacttag ttactttagg tagagcttca agagaagtag aaagaataaa
     6181 agttcgtcaa ccattacaaa aagtgttagt agatggtaag tttgaagata caataagtga
     6241 tgtagttgat ttaataaaag aagagcttaa tgttaaagag gtaatatttg ctaaggattt
     6301 agatgagtat atgaacttta gtttaaaacc aaactttaag gaagcagggc cattattagg
     6361 ttctaagatg aatttattcg ttggagcatt aagtaaatta aatgctcatg aaactgctaa
     6421 taaacttgaa aaaggtgaga ctttaacagt tgatttagat ggcgaagctt ttgaatttaa
     6481 taaggattta gtattaatag gtataactgc taaggaagga tttaatgtat ctgttgaaaa
     6541 caatcttttt gttatactag atactaagct aactgaagag ttgattaatg aaggttatgc
     6601 tagagaattt atatctaaag tacagcaatt aagaaaatct aataactttg aagtattaga
     6661 taatatagta atagaatact gtggtgatga tgaaattgct aaggctgttg accattttaa
     6721 tgaatatatc aagtctgaaa ctttagcttt agaaataaac agagtagatg ataaatcttt
     6781 agaggaacaa aacttaaatg accatatgac aggtataaaa gttataaaaa aataaaaa
//
LOCUS       DAEPOG010000187         6790 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEPOG010000187 DAEPOG010000000
VERSION     DAEPOG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.187"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(204..812)
                     /locus_tag="KR432_003196"
     CDS             complement(204..812)
                     /locus_tag="KR432_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBH0641707.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(921..3659)
                     /locus_tag="KR432_003197"
     CDS             complement(921..3659)
                     /locus_tag="KR432_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH0641708.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3807..4598)
                     /locus_tag="KR432_003198"
     CDS             complement(3807..4598)
                     /locus_tag="KR432_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0641709.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4634..5290)
                     /locus_tag="KR432_003199"
     CDS             complement(4634..5290)
                     /locus_tag="KR432_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641710.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5283..6248)
                     /locus_tag="KR432_003200"
     CDS             complement(5283..6248)
                     /locus_tag="KR432_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBH0641711.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6354..6460)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2184 a         1130 c          609 g         2867 t
ORIGIN      
        1 ataaaataca caaaaaaatt aatttttata gctcatttcg ccaaaatata tctttcttta
       61 tatcattaaa ataattataa atttattaaa tatgtttgta attctataaa gttattttta
      121 tcaaactaaa aattctataa aaaaataaga tacataccta taaaatacct tcctatagat
      181 acatatctta cctatataaa aacttattct atataaaaac gccataaaaa atcttttgct
      241 tcttctgcat aatctatatt tatacgttta ctagttttta tatctaactc atctttacta
      301 aatacttttt ttcctttatc atcataataa aaatcactta tatataattc ttcacccatt
      361 atactcttac tatttagact tctatctatt tttaaagctt tgcaaagttt tcctggtccg
      421 ttagtaatat tttttacttg atatttactc aactcagtgt aagctttgtt gtatctattc
      481 atagatatct catctaaaga agttataggc tctacccctc ttattaatac acactctggc
      541 acattttcta tatttgcaga taaatttaag caattataca taccataaat taaataaaca
      601 tatatatgtc caccatctaa atataatggc ttagttctat ctgtcttttt attgccatat
      661 acatgtgctc ctttatcatt tacacccatg tatgcttctg tctctatgat tttggtaaca
      721 attactttat tttcatattt ccttattaga tatttaccta atattgattt ggctaaatca
      781 attccatttt ttctaaaaaa atccttttcc aaaatttcac ctcataaaat ttatttaaaa
      841 cgcatcaaat acaactaaag taataaataa aattaactct tattaatgtg atatatataa
      901 acaataaatt tagccattta ttatttatat atatcaaaaa attctatctc aaacttttca
      961 atattatcat tgtaatttga attatcatat acttcttcaa caacctcttt aattgggaat
     1021 ataactttta cattagttcc ttctcctacc ttactatcta tgtaaatctt cccattttga
     1081 atttcaatca atgattttgt aaggcataaa cctataccac ttccaaattc atttgatata
     1141 ccactgtttt caatttgttt aaatcgatta aatatatcat ttaatttttc tttgtctatg
     1201 cccactccag tatcttttat aattatctct actgtttcgt cttttttata tatacctaca
     1261 aggatttctc ctccttctaa agtaaatttt atagcatttg ataatatatt tagtattatt
     1321 ctttctatag attctgggtc aagccctacc ataacagttt cttcatccgt atcaaaaatt
     1381 aaatctattc ctttagactt agcataacta ctaatagatg taactattct ttctgttatg
     1441 tcaataatat caaaattaac tgctttaatc ttatatatgt ctgacttaac ttgagaaata
     1501 tttattatat tatctattaa tctcaataat ctaaacatat tttgtttagt tatttttata
     1561 tgttctttta gtatatcctg aaaatcaatt atttttcctt ttttataaag gttttcaata
     1621 agttgaactg aactatatat attatttaaa ggtgtcttaa gttcatgaga tatattagca
     1681 taaaactcca tcttcatttg gtcatactgt ctttgtttta ttaatgcttt tttaagtctt
     1741 tcagactctc tttgcacact taaatcttta ataattctca ctctacttct tttctgtcca
     1801 aagtataatg ttcttgatat catttttact tctttaatgt gattattttc atttactata
     1861 tggatactct tctctctttc tcctatattt aaattgttta tgtatttttt tactaatata
     1921 tttctatatt tttgtgttgt aactcctaat acatcactag aaattccgaa caactctcta
     1981 aaggctctat ttgtatatag tactcttgat gtacttaaat cctcaagaaa aatagcctct
     2041 ggtataacat caagtaattt ttcatatatt attttttgtt catttactgt tctaagaata
     2101 tttatctctt tagttatatc tctaaatgta agcaatttaa agagtttatt atctgaccaa
     2161 tagtctccaa ctctaacttc ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa
     2221 ttaactatac agtttgctgt gtcattgtaa cttactattt cagctttctt ttcttgtgtt
     2281 agatttaatc ttttaaattt cctattgtca aagaaatcta tgttaaaagt ttcaaaatat
     2341 tgttctaatg tcatatcgac taaatcactt gaaacacatt taaaactctt ttcatatttt
     2401 ttatttatac ttattacctt attcttcttt atgccacctt cagcacttct tacaagtata
     2461 ggagatggaa tatttagtat aatgttttca tagtctttat ttatatttct tatttcttca
     2521 tctattttga tttcatttgt aatatcattt aaacttatta agtaattgct ttcatcttta
     2581 ttattactta aacttaacaa ataaatttta tcatttaact ctaccagttt atcaattata
     2641 cttatatcgt tatcaaattt cttcaatttt tcagcaactt tttcatcttc ctttataaaa
     2701 gttaatattt tataataatt attcatatcc tttgaaaatt catctttaaa tttattattt
     2761 gtgtaactta tattaaaatc atagtctgtt actatccata catttggtat tgtatccaga
     2821 gcttgtctaa gtaattcttt tctctggtct atgtagtttt ttaatgttct ttgtataatc
     2881 atatcctttt ctattgctaa atttatcttt cctaattgct ctgacttttt acttaatttt
     2941 ttattgattg aatcaagttc ataatatgga tacactactc tattattaag actgtataca
     3001 tatacataat aagtatggtt acataatagt aaatatatta ctcctattaa tctatctgaa
     3061 tctactatat taatcttaga aattgctaca aaacatataa tagttgtaaa aaaaacaaaa
     3121 tatgttaaat cttttattaa cttatagtgc tcaatgttac tatcaagttt attcaacaaa
     3181 tatagtaaat taaacataga tatacaaata ttaattaata taataatcat attccagtta
     3241 atatcactaa aaatatttct tataaagtaa tctaatatca caaaagcact ataaaaaatt
     3301 agtattgggc ttattcttag tttttttact ttagaaatgg ttaagtatat aaaagtagac
     3361 atattaatta tgcttaagtt aagcaataca atttggaatt tttgaatttt taaatttact
     3421 actcctaaaa tacttacaaa aactaataca agacacccta ctttcaatat atttataaat
     3481 tcatctgttt taaagctact tatataaact atagaatatc caaatatcca aagtaaaatt
     3541 attctataca tatttataga aactgtatac aaatttatat ttaaaagtgc aataaccaaa
     3601 aataacccat tagctagtat atagacctta ttcttactta aagttccttt agattccata
     3661 ttccccacca cttcttcatt tttatatagt tttacctata aatatgttac cacaattctt
     3721 ccaaaaaatt tgtcactttc tagttataaa aaaaaatgag taaatatttt taattttact
     3781 cattttgtag attttgtata ttcagtttaa aaagctggaa ctatacttcc tttatattca
     3841 tcttgtataa attttttaac ttctttacta ttcatagcct ctgataaagc cttaatctta
     3901 tcactatctt tattattttc tctacaagct attatattta catatggaga atcacttgat
     3961 tctattacta ttgcatcctt agtaggattt aagttagctg ttaaagcata attagaattt
     4021 attactgctc cgtcaacatc ttttaatact gttggtaatt gctctgcatt catttctttt
     4081 attactatat ttttaggatt tttagttata tcttttttag ttataagctc tccatctttt
     4141 acttctatca atttattttt tgctaataat ttaagtgctc tagctccatt agttgcatca
     4201 tttggcacag ctataacagc tccatctttt aattcatcta atgacttaac tttttctgaa
     4261 tagaatccca ttggctctat atgaacttta gatgtataag taagtttata tcctttctcc
     4321 ttcacagttt cctctaaaaa tggaatatgt tgaaagaagt tagcatccaa agacccttca
     4381 tctaaagctg tatttggaag tacataatca tcaaatattt ttacttctaa atcatatcct
     4441 ttttctttta acaaaggttt tgcaacttcc aatatctttg catgtggatt tgatgaagct
     4501 cctactagta tttttttatc ctctttgttt gaacaaccta ctgctgatat tgctattgct
     4561 gacaccaatg ctactgataa taatttcttt aatttcattt ttactccccc tctttaaaat
     4621 gtttataatt aatttatttt agtttcttat aagccagatt tcctactccc tggattatct
     4681 gaactaatat tataagtgct attacagtat ataccattac tgctgtatca aatctttgat
     4741 atccataaat aagtgctata ttacctaatc ctccagctcc tactgcacct gccatagctg
     4801 tataaccaag tatagatatt attgataatg taattccaga tactattgaa ggcatagctt
     4861 cctttatcat aactttaaat actatttgtc tttttgtagc tccaaaagat tttgctgctt
     4921 ctatcaatcc tttatcaact tcatttaatg agctttctat aattctagcg ataaacggtg
     4981 ctgccccaat agttattggc actattgcag cagtttcccc tatacttgtt ccaacgatta
     5041 atctagttat tggaatcatt gcaactataa gtatcataaa tgggaaactt ctaaatatat
     5101 ttactataaa tcctaatgca ctatttattg tgctatttgg ttttaatcca tctggttttg
     5161 taactactaa tatgattgct aatataaatc ctaatattgt tgccactata gttggtacta
     5221 taaccatata caaagtttgt aacaatgcat ttggaaatag tgttgttaaa aagtcaatta
     5281 aactattcat ttatcatttc ctcccatctt atgcccttac tagttagata ttgttttacg
     5341 tgctctccac ttttatctga aatgtttata ataagtgaac ctaaaatatc atctttaaat
     5401 tgttctaatt tcccaaatat tatagacaca tctatattta gttctcttgc catagtcgta
     5461 ataatagctt cattagaaat atctttaggg aatagtatct ttatatttgt tccttttggc
     5521 aatattatac tttcttcacc tattaatttt ctaagtcctt tagtatttct taagaatatt
     5581 tcttctgtat ctcctacctc taatacttct ccattttcca ttattgctac tcttccacaa
     5641 atctgcttta taacttccat ttgatgagtt acaactatta ttgttattcc caatttttta
     5701 tttatatctt caagcaatga aagaattgac tttgtagtat ttgggtctaa tgctgaagtt
     5761 gcttcatcac aaagaagtac ttgtgggttt agtgctaaag ctctagcaat tgcaaccctt
     5821 tgtttttgtc cgccacttag atttctgggt ttatcatttt tcttttcaga tattccaact
     5881 acttctaata actctaaaac tcttttttta atttctgcct ttgagtatcc aaaacattct
     5941 aatggcaatg ctacattatc aaatacagtt tttctttcaa gcaatgaaaa gtgttgaaaa
     6001 atcattccta actcttttct taaatctctc atttgctttt cattcaatga ctttacttct
     6061 ttatcagaaa caagtacact gccttcttgg tattcttcta atccgtttat acatctcagt
     6121 agtgttgatt tccctgcacc actatgacct attattccaa aaatttctcc agattctatt
     6181 tcaatactta catctttaag cacttgaatc tttccataat acttattaac attttttatg
     6241 ctaatcataa aatttcctcc catatattct gctcagtttt tatgtttatc ctctcttatg
     6301 tattacaaca aaataaaagc ccgagtataa actcaggctt agaaagctat ttatactcat
     6361 ctatcaacta tattagttgc aggatttagc accaatgtat ataaaatata ctggttgccg
     6421 ggtttcaaag ggccagtccc tccaccactc ttgataagac gttatttaat tcaattctat
     6481 tatcatttgt tgctatgtta tatatgatat attcattaat agtatttgtc aataaattta
     6541 tttaaatttt ttatttttta ttatcgttaa ttaactattt ttaattagtt ttctagctcc
     6601 taacacttta tattttaatg tctatataac tatagaacat cattataaga aataaattaa
     6661 ataacatcaa tctatctaaa tcatctgtaa agtaaattaa gaaaattaac attttatcca
     6721 aactctatcc tactattaaa taaaataatt taaatacaat gatgattata tagctctaat
     6781 actttagact
//
LOCUS       DAEPOG010000188         6607 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEPOG010000188 DAEPOG010000000
VERSION     DAEPOG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.188"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(139..978)
                     /locus_tag="KR432_003201"
     CDS             complement(139..978)
                     /locus_tag="KR432_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0641712.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1002..1760)
                     /locus_tag="KR432_003202"
     CDS             complement(1002..1760)
                     /locus_tag="KR432_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0641713.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1807..2280)
                     /locus_tag="KR432_003203"
     CDS             complement(1807..2280)
                     /locus_tag="KR432_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0641714.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2309..2725)
                     /locus_tag="KR432_003204"
     CDS             complement(2309..2725)
                     /locus_tag="KR432_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0641715.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2772..3824)
                     /locus_tag="KR432_003205"
     CDS             complement(2772..3824)
                     /locus_tag="KR432_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0641716.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4220..4900
                     /locus_tag="KR432_003206"
     CDS             4220..4900
                     /locus_tag="KR432_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0641717.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5033..5353)
                     /gene="sugE"
                     /locus_tag="KR432_003207"
     CDS             complement(5033..5353)
                     /gene="sugE"
                     /locus_tag="KR432_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH0641718.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5735..6562
                     /locus_tag="KR432_003208"
     CDS             5735..6562
                     /locus_tag="KR432_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0641719.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2228 a         1228 c          797 g         2354 t
ORIGIN      
        1 tagcttatat aagtttaaaa tttaatctat tataaaatta ccctaattgc taaacttttt
       61 aatataaatt ttccaacatc ttacaaatat aatcaataga tatagaatcc taatttacac
      121 accaacatat atatttattt aaaatagtcc caaaatacct gtaatagctc ccactactat
      181 cagtataaac attattttta ttggagaaag gccttttttt aaagcaaaga gtactaataa
      241 agtaagtacc aaaggaatta atcctggaaa tatcttatca agtaaatccg tttgaagact
      301 aaatttaaaa tcaccactag tataacttat tggtgtagat aaattaataa acctacctac
      361 aagtcccccc atgactaaag tacctaatat agaagcccct tctataacct tatttactat
      421 tccaccttga agtatacttt caacagcttt tttcccatac ttatatccat ttgtccacat
      481 tccaaaagaa attgaagtca ttattatata ttgagctatt acaaataata taggacctgc
      541 aattgatgct cctccctcag ttaaaccaat acaaacagct aacactatag gagttataac
      601 tccttgagtt aaagtatcac caattcctgc tagtggaccc atcaagcctg ttttgatagc
      661 attcatacca tctccactta tttcatctgc tccattagct ttttcttctt ccatagctat
      721 tgttattcca tgtataacac atccacatat aggttctgtg ttaaaaaatg ctaaatgagt
      781 ctccacttct tgttttaact cttccttatc tggatataat tttttaagta ctggcaacat
      841 tgaatgtgag aaagctgttg attgcaatct ttcataattg taattagatt gcgcaaagaa
      901 aaaccatctt aaccatgatt ttattacatc ttttttgctt aacgttttct tagatacttc
      961 attttttata tctgacataa tattttcccc ctcacatgta tttaagatgt agcactatct
     1021 tttccgtact taattgaagt aattgtaaga gcaagtatta ctccaataac agatattact
     1081 attatatcta ctttaaaata aacagccatt agtattccta atatgaaaaa gggcatagta
     1141 aacttgtttc caatcaattt taaattcata gcaatcccaa gtgctggcaa catagcccca
     1201 attacattaa atacatgtaa cacattctca ttcattactt ccaacatacc atctatcgca
     1261 tttggaccaa aataaacaac taataatgct ggtataaaac tcattgcaaa taacagtatc
     1321 tgtggtggta ctatatttat aaatgctact ctatcaacat tccctttttg aacctctcta
     1381 tcagcccaat gtgcaaaaaa agaatttact gtcatttttc caatccaaag tacagttgca
     1441 acagttccca atggtacagc tattgccatt gcagtactta catctagctt agatgctata
     1501 gctaaagcag tacctacata tcctgcaaaa gctgggtccc ccatctgagc tccccctgct
     1561 gatataaaac ctagatatat catatttata cctgctccta taattgctcc ttgaacaggg
     1621 tctcctaata taattcccgt tacaaatcca gccaatagag gtctattcca tgtaaaaaat
     1681 ccaacatagg tcaaccacgg actccaagaa agataataaa aaattgctat taatactgct
     1741 tgaattagtg aaatgtgcat attcttcctc cctattttct tttaaagatt tacaagatag
     1801 tcttttttat aataatgttc ctacatcaat tttctttgca tctggtacta tttgtataaa
     1861 gcatttaaca tcttttgata ttagttctct aaatatttct ttttcttcat ctgacacaga
     1921 tatattttta taaaaacttt ttctaccaag ccctgctccc atacctccaa cattcaattc
     1981 ctttaaatct accccactat ttactaaacc taagacagtt ttagggtatt tgaccaatac
     2041 tattaaatgt tcatctccat tatgtgattt taaaacttca gcacctgatt ctacatcatg
     2101 tacttcaacc ttaacagttg atggagctgc catttgtaaa acttgaacta taaatgggtc
     2161 ctttactact aaatcatcca cgattactat cctttttgcc gatgtaatct tacaccaagc
     2221 agtaataact tgcccatgta ttaatctatc atcaattctt gttaatgcta tattactcat
     2281 aattattcct ccagattttt aatttatttt acaactttaa aacagtttta acatctttga
     2341 ttcctgatat cccaacttcc tcacataaat tcttcaagtc ttttaacttc atagtatctc
     2401 tgtttaaaaa tagctctaaa agcataggta aatttacacc tgtcaaacat tctaactcac
     2461 ataccttagc aaatttattt attataatag cagatgtatt aaatggagtc ccgccataaa
     2521 aatcaagcaa tactaaaacc ccttcatcct tatatttctc aattctatct tctaaccttc
     2581 tactaaactc atctatgctg tcaccatggt ttaatcctaa agtttctaca ttttcctgtt
     2641 tgcctactat taattcagca ctttctaaca ttgctttagc catatttcca tgtgtggcta
     2701 taattatccc tgtcatatga atcatacctt tcttaacctt gtttatatgg attattattt
     2761 agtactctaa tctaattatc tatattaatc attatcttac caaagaaaat atcccttttg
     2821 tatacttttt caaaataagc tggtacttct tctaaattta ttctatgtgt tactaatttt
     2881 tcaacattca tatcaccctt ttgcatatga tacaatccag cactccattc ttttcctggg
     2941 aatggaaaag agtttccaaa ccaagttccc ttgacagtta attcacttct tactatcttt
     3001 tcaaattgtt ctctagtcaa agctacatcc ccatatggta cacctgcata caatactgtc
     3061 cctccctttt tagctaacaa caatacttgc ccacatgtca ttggagtccc agccgattca
     3121 ataacaatat ctgcaccatc tccatcagtt aaccttttta tttcttctac aatattcttt
     3181 tcttttgcat ttatacaaat atctgcccct aactctttcg ccaagtctaa cttctcatca
     3241 aatacatcta cagctattat ttttgtagag ccaaatatct ttgcccattg tattgaaaat
     3301 aatcctattg tacctgtacc aagtactaca acagtatctc caactttagc ttctgatcta
     3361 aaaagaccat gccctgctat acatactggt tctagcgcag cggcagtctc ataacttatc
     3421 tcatctggta tttttattaa attcctctct ttcacctttg tatactctgc aaaacaacct
     3481 ccaagttctt tatttcctat tattgctaca ttattacatc ttgaatacaa tcctttttta
     3541 cattcatcac attcaaaaca tggcatagct ggacaaacag caactctatc tcctatttta
     3601 aaactcctta cttctttacc aacctgtgcc acttctccag aaaattcatg ccctaaaatt
     3661 tctccaacca tatgtggacc tgtctttgaa tattttgata tatcagaacc acatattcca
     3721 gctactttta ctttaattat tacatcatct ttttctaata tttttggtac atccacatcc
     3781 tccactcttg tgtctcttat accatagaat ctaactgact tcactattaa ccacccctac
     3841 tttttatttg agaatatatc cctattgttt atatgttaaa tatctaaagc aactttcatg
     3901 ccaatatatg taagcattgc attcatgcat tttataaaat tatcattaaa tgtgtgtcaa
     3961 agtgtgtcaa aagtttctta tattaaaact tatgtgtgtc aatataaacg acactaacat
     4021 tttatattag ttctcttatt ataagtttta taaaatgtac tttttttgtt atttttataa
     4081 aattgttcgg aaatttccct tctatttgtg atataatatt aaatataagg tatcaatata
     4141 aattttcatg cgaatatcag aacttttttg acactaatgc cttattcaat ataataattt
     4201 aactttttgg aggttgttta tgaaaaaagt gtatcaagga aagactaaag atgtttttga
     4261 attagataat ggaaattact tacttaaatt taaagacgat gtaactggta aagatggagt
     4321 atttgaccca ggcgaaaata gtgtaggtct aagtatagat ggaattggta gagctaattt
     4381 agagacatct gttaagttct ttgaaattct aaacaatgct ggaattaaaa ctcattatgt
     4441 aagtgctaat ttagaagaag caactatgga agttctacca gcaaaagttt ttggtaatgg
     4501 attagaagtt atttgtcgtt atagagctgt tggtagtttc ttacgtcgtt atggttcatg
     4561 tgttgaagaa ggtgctaaac tagattgcta tgtagaagca actttaaaag atgatgaccg
     4621 ttgtgaccct cttataacat ctgaaggttt agaagcttta aacattatga ctcaagctca
     4681 atttgattca atgaaaagca tgactcaaaa aatttctaat atagttcgtg atactatagc
     4741 tgaaaaaggt cttgaacttt atgatatcaa atttgaattt ggattctaca atgatgaagt
     4801 aatcttaata gatgaaatag catctggaaa tatgcgtgtt tataaagatg gtgtaatagt
     4861 tgaccctatg gatttaactg ctcttttatt agataaatag tatatacata taattttcaa
     4921 atataataaa cttataaata agataagcta tgaagtaaag actttttaaa tgttgattat
     4981 ttttaaacat tttctcgcct ttaatttcat agcttattta aattaatata ctctaattag
     5041 tagataatag ctttaaccct acaattccca ccacaataaa gcctatacat gcaattcttg
     5101 atatactaat aggctcattt aagatagcca tgcctacaat agctgtaccg acggtaccaa
     5161 ttccagtcca tattgcatat gaagttccaa gtggaagatg ttttagtgct aatgacaaaa
     5221 aataaaaact tacaatcatt ccaacaacag taagtatact tggagttaat ttagaaaatc
     5281 catgtgaatt ttttagttga aaagcccaaa acacttcaaa caaacctgca acaaacaaaa
     5341 ataaccattt cataaactca tccctctttt caaatgtatc tcttattttt ccaataaaat
     5401 aacctgagaa atattataaa atatctccca ggtttttgtc cttccgtgtt cacaacaaaa
     5461 actatgttgt gggttttatc tcggaccagg ccaatcatta ataattgcaa aaccctataa
     5521 aactctatta attattctat tttatctata ataatactat caacatatat aaaaaaagtc
     5581 aatatatggg ttccatctat aataaaaata acttttatat accttttaat attcttattc
     5641 acttctctcc atacccattt aggtcaatct aagaatatct aatttttatt taatatgtta
     5701 taataacata aaaatattga aaggagtaat ttctatgaat ttctataaaa ttgcagtatg
     5761 tcagatgatg accacagaaa ataaaattga aaatatcaat catgctgtag atatggtaac
     5821 agaagctgcc atcaatggag ctaaaattat agttttgcca gaaatgttta actgcccata
     5881 tgaaaataaa tattttccta aatttgctga agaatatcca ggtgaaacaa caacgatatt
     5941 gagtaaatta gctgagaaac atggaattta tctagtttct ggtagtattc ctgagttaga
     6001 atatggaaaa atctataata cttgctatgt atttgataaa aatggagctc taattggaaa
     6061 acatcgtaaa atgcacttat ttgacattga agttacagga aaagttagtt ttaaagaatc
     6121 agatactctt acagctggaa atgatgtcac tgtaatagat accgaatatg gaaaagttgg
     6181 tatagctatt tgctatgata ttcgtttccc tgagttatca cgtttaatgg ctcttaaagg
     6241 tgttgaaatc gttatcttac ctgctgcatt taatatgact actggtcccg cccattggga
     6301 gttatctata agaatgcgtg ctttagataa tcagatattc tatgttggtg ctgcacctgc
     6361 tagaaatatg aatgcttctt atatagcttt tggaaattca agaatttcag acccttgggg
     6421 aagaataatt gctcaagctg atgaaaaaga atgtattatt tatgctgaca ttgatagaga
     6481 tttaattcct gacataagac aacagttacc acttcttaag cacagaagaa ctgatttata
     6541 tgaacttaat actttgaaat aaaacagaac ataaccccat aaatcataaa ttaagtgtta
     6601 aaaaaat
//
LOCUS       DAEPOG010000189         6577 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEPOG010000189 DAEPOG010000000
VERSION     DAEPOG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.189"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            255..1199
                     /locus_tag="KR432_003209"
     CDS             255..1199
                     /locus_tag="KR432_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBH0641720.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1221..1847
                     /locus_tag="KR432_003210"
     CDS             1221..1847
                     /locus_tag="KR432_003210"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH0641721.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1881..2930
                     /gene="hisC"
                     /locus_tag="KR432_003211"
     CDS             1881..2930
                     /gene="hisC"
                     /locus_tag="KR432_003211"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0641722.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2933..3511
                     /gene="hisB"
                     /locus_tag="KR432_003212"
     CDS             2933..3511
                     /gene="hisB"
                     /locus_tag="KR432_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBH0641723.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3513..4118
                     /gene="hisH"
                     /locus_tag="KR432_003213"
     CDS             3513..4118
                     /gene="hisH"
                     /locus_tag="KR432_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH0641724.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4115..4837
                     /gene="hisA"
                     /locus_tag="KR432_003214"
     CDS             4115..4837
                     /gene="hisA"
                     /locus_tag="KR432_003214"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH0641725.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4819..5577
                     /gene="hisF"
                     /locus_tag="KR432_003215"
     CDS             4819..5577
                     /gene="hisF"
                     /locus_tag="KR432_003215"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH0641726.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5605..6273
                     /locus_tag="KR432_003216"
     CDS             5605..6273
                     /locus_tag="KR432_003216"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH0641727.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2695 a          549 c         1275 g         2058 t
ORIGIN      
        1 attatcatgg tgagattaaa tatagtaaaa ttaaatacaa aattaaatca tcaccatcat
       61 catagattaa agggattgta atatgaaatt aattcataga cacaagccct gtttttagtg
      121 taattggtct tgtatctatg atgggaagta attttaggag aaaattatag ccatataggt
      181 ataaagccta tatggctttt ttattgtttt aaatttgcaa atataagagt aaatttaatg
      241 aagagggtga gaatgtgaaa tttttgagga ttgaagatga agttatttat tctaaaaaaa
      301 gatataaatt aaaaagggaa atagataaac tttttttgga tgaagaatat tttcaaatag
      361 aaccacaatt atttgaagaa tatgatgaat ttacaactat aaataataaa attccagaag
      421 aatctatggt aaaggttgta aatggaaaag taatggtact tagagctgat ataactacaa
      481 gtataataaa aagccttgta ccaagatggg aagatggttt aaagcttaag cttttttata
      541 attcatcaat atataaaaat aaaaatacag ttggaataaa agagataaga caaataggtt
      601 gcgagtatct aggagaagcc tctgtagaag cagatagaga agtagtgaaa ttagctctta
      661 agatacttga aaaatacaat aataatttta ttttagaggt tgggagtagc aagtatattc
      721 atggtttgtt agaagaatta aatttaaata agaattgtga aaatcaaatt aaaaatttac
      781 tatatacgaa gaacactcat gaattaaaag tatatattga agaactgaaa attaagcgtg
      841 aagtaaaaga gctattatca aatattttaa gcttgcaagg gaatttatac aacgttattg
      901 aaaaatcaaa caagttctat tgtaataata agatgaagca agctcttgaa gaattaaagc
      961 aagtaaataa tttaattgag gaatgtaatt ttttagataa ggctagattt gatttatcaa
     1021 tgattactat gcttgactat tatgaaggaa ttatgtttag aggatactac ccaaactcat
     1081 ataaagaaat tcttagtggt ggaagatatg attctttaac taaagagtat ggaaaagaaa
     1141 ttccagccat tggatttaca ctaagtgttg atgagttaat gaagtatgta tataagtaaa
     1201 gtttagaagg agggattgct ttggattatt tgactatagc actagcaaaa ggacgaattg
     1261 aaggggaatc atttaaaaaa tttaaaaaaa tgggtctagg agatagtata gacacagata
     1321 cgagaaaact aatttttaaa gatgaagaaa ataaaattat atatatacat gtaaagccat
     1381 cagatgtagt tacatatgtt gaaaaaggag tcgctgacct tggtatagca ggtaaagata
     1441 ctattttaga gaatgagaca gatgtatatg aaatatatga tttaggtttt ggaaaatgta
     1501 aatttgctgt agcaggatta aagggagata gcatatatag agaagatgaa tatctaaaag
     1561 tggcaacaaa gtatcccaat atagcaaaaa agtattttaa agagaaaggt caaaaaattg
     1621 aaataataaa attaaatggt tctgtagaac tagctccaat agtagggttg tcagatgtaa
     1681 ttgtagatat agttgagact ggcaatacac ttaaagcaaa tggtcttgaa atattagaag
     1741 acatatgtaa tataagtgct agaattatat caaatagagc tagctataga tttaaatatg
     1801 aacaaattca gaatataatt cgtttatttg aagaacttga taattaaaat ataattgaac
     1861 tttataagga gggatttaag ttgagagaaa aagaaagtat aagagagtta aggggatatg
     1921 aaccaaatca cgtaaactgt aaagtaaaac ttgatgcaaa tgaaggaagt aagaggttgt
     1981 ttaaatacct tattaaagaa atttcagata gtgatataga tttaaattta tatcctgaag
     2041 attcttatag tgatttgaaa gaatctataa tagattacat aaatatttct ggagtaaata
     2101 agaaaaatct tctagtagga aacggttcaa gtgaaataat tgatttaata atacatactt
     2161 ttgtagataa agatgaagta atattgagtt tctctccaag ttttagtatg tattctatat
     2221 atagtcagat aaatgggtct aaatttatag gtgttgaaag tgatgaaaat ttagttataa
     2281 atatagataa tgtgattgaa aaagtaaagg agaataatcc taaaatagta attgtatgta
     2341 atcctaataa tccaactgga acaatattaa agagggaaga gataataaag cttttagatt
     2401 caactaatag tctagtggtt ttagatgaag catatatgga ttttggagaa gaaagcatgt
     2461 taagtgatgt ttttaagtat gataatctaa tagttcttag aactttatca aaggcatttg
     2521 gattggctgg aataagaaca ggatatatgt tatctaatag tagtttaata aattctgttg
     2581 agaaagtaag acctccatat aatttaaatt cattatcaga ctttattgct acaagagcac
     2641 ttagaaataa agatgtggta aaggcttata taaaggaagt aaaagaggaa agagaagttt
     2701 tatatgaaga aatgattggt atgggaatta aagcatacaa atcacaagca aattttatat
     2761 tattttattc tgaaatcgaa aatttatcac aaaaacttat tgatagagga gttttaataa
     2821 gaaaatttgg tggaaagtta gaaaattatt acagagttac aattggtgat aaagaagaaa
     2881 attctatgtt tgttggagct ataagagata ttttgaaaaa ggagaaatag gtatgagaat
     2941 ttggaaggta gaaagaaata cttttgaaac acagatttta gtagaattaa atattgatgg
     3001 ttctggtaaa gctgaaatag atactggaat aggattttta gaccatatgc ttaccttaat
     3061 gtcatttcat ggcaagttcg atttaaaagt gatttgtaag ggtgatacat atgtagatga
     3121 ccaccatagt gtagaagata taggtatagc tataggtgaa gcatttaaaa atgcactagg
     3181 tgacaaaaaa ggaatacgaa gatattctaa tatctatata cctatggatg aatctctatc
     3241 aatggtagca atagatataa gtaatagacc ttatcttgta tttaatgcta aatttgatac
     3301 ccaaatgatt ggtagtatga gtactcaatg ctttaaagaa ttttttagag cttttgtaaa
     3361 tgaatcgaga gtaacattac atatcaatct cttatatggg gaaaatgacc atcataagat
     3421 agaaagtatt tttaaagcat ttgcaagagc tttaaaagag ggtagtgaaa tagtttctaa
     3481 tgagatagca tcttcaaagg gggttttgta gaatgaatat aatagtagac tatggtcttg
     3541 gaaatataga ttctgtgtct aggggtttta gaaaagctgg aatagaaact aaaatttcta
     3601 gtgatataga tgaaataaaa caggcagatt cacttatact tccaggtgtt ggagctttta
     3661 gagattctat aagtgcctta gataaactag gattgatacc aataataaag gagcatgttt
     3721 ctaagggtaa atttatgata ggtatatgtc taggaatgca attactttat gaaaaaagtt
     3781 atgagtatgg agaatatgaa ggattaggac ttataaaagg aagtattgat aaactggata
     3841 taactttaaa agtgccacat atgggatgga ataatctaaa atttaataag gcaaatgatg
     3901 atatacttaa atatataaac gaagatgatt atgtatactt cgttcactca tattatgcaa
     3961 attcatcaaa tgaagaacta gttgcatttt cagaatatga aaagaaaatc cctgctattg
     4021 ttagaaaagg caatgtatat ggtatacaat ttcatcctga aaaaagtgga gaagtagggt
     4081 taaatatatt aagagcatat ggggagatga taaaatgata atatttccag caatagatat
     4141 aaaagataat aagtgtgtaa gactcacaca aggagaattt gacaaagtaa atgtatatta
     4201 tgataatcca ttagaagtag catataaatg gaaaaatgaa ggagcagaat atatacatat
     4261 agtagattta aatggtgcta gaagtgaatt tggtataaat acaaaaataa ttgaggatat
     4321 agcaaacaat atagatatac ctattcaagt tggtggtgga gttagagata agggaaaagt
     4381 caaaagttta ataaatgctg gtgttactag agtaatatta ggtagtatag cgattgaaaa
     4441 tttgaatttg gttgaagaat tggtcaatga atataaagag aaaattgtag tttctataga
     4501 cgctaaagat ggaaaagtag cagttagggg atgggaagtt gtaagcgatg tagactcatt
     4561 aacattatgt aaacaacttg aaaagatagg tgtacaaact attgtttata cagatatatc
     4621 aaaagatggg atgttgcaag ggccaaattt tgacatatat gaaagaatag caaaggaaac
     4681 gtcattaaat gttattgctt ctggaggcgt aacttctata gaagacgtaa aaagactaaa
     4741 agctatgaac ttatatggtg caataatagg taaagcgctt tatgataaaa agatagactt
     4801 taaggaggca caacaattat gcttactagg agaataatac cttgtctaga tgtgagaaat
     4861 ggaagagttg taaagggaaa aaaatttaaa gatatagtag atgttgatag tccagaagta
     4921 cttggaaaat tttatagtga ttgtggtgca gatgaacttg tattctatga tataactgca
     4981 tcaaatgaag agagaaagac atctttagaa tttgtgacaa aagttgcaga aaatataaat
     5041 ataccatttt gtgttggtgg aggagtaaat aaattagaag atttcactga tattttaaga
     5101 aaaggtgctg ataaggtttc tataaattca tcagcagtca aaaatcctga attaataagg
     5161 gaagcttcat taaaatttgg agcacaatgt gttgttttat cgatagatgc aaagaaaaat
     5221 gaagagggtt catggagcgt atacgtaaaa ggtggaagag aaaaaactaa tctggatgca
     5281 attgaatggg ctgttaaagg tgtagaactt ggagcaggag aaattgttgt aaatagtatg
     5341 gacgaagatg gaatgaaaaa tggatatgat atagaattat tatcaaagat aacttcttta
     5401 gtaaatgtac ctgtcatagc atctggaggc gctggaaaaa aggaagattt ttatgaggca
     5461 gttaataaat ctaatgtaga tggaatttta gcagcatctg tttttcattt tggcgagata
     5521 aaaataaacg atttaaagaa atatttaaaa aacatgggtg tagaagtaag attataagtt
     5581 aaatactaaa aggagattag aaatatggat aataagtgta acaatgtata cagtgatgaa
     5641 gtagaggaat ttataagaag tataaagttt gatgataaag ggttagtccc tgttgttgtt
     5701 caagaggtag ttagtaaaga tgtactgatg ttagcatata tgaataaaga tgctataaag
     5761 aaaaccttaa aagataaagt agcttgctat tttagtagaa gtagacaaaa actttgggtt
     5821 aaaggtgaga catctggtaa tactcaaaaa gtagttaaga tgtcttatga ttgtgatgta
     5881 gatactatac tactttttgt tgaacaaact ggtgtagctt gtcatacagg caattattct
     5941 tgtttttaca gagacttgtt tgatgatagt tcaaaaatgg agcctgaagt acaaacaaat
     6001 atattgaaag aattatatga tttaatcaat gaaagaaaaa ataatccagt tgaaggttct
     6061 tatactaatt atctttttga aaaaggaata gataagattc ttaaaaaagt aggtgaagag
     6121 agtagtgagg taattatagc atcaaaaaat accgataaaa gtgaacttat ttacgaaatt
     6181 agcgatttag tgtatcatac attggtttta atgatagaaa aaggtgttga aattgatgaa
     6241 ataaaaaaag aattacttaa aagaagaaaa tagaatagga tagattaatg gtaataagaa
     6301 aatataccgc ttttaataaa aagtggtata ttttttattt tatattttcg atttatagaa
     6361 taatatttaa attacttgat aaaattaact atatatataa tttattgata aaataattgt
     6421 agtacattat ttattttaga aaaaagataa ttatctaatt atatttaagg ataataatat
     6481 gaaaataatt tttatattaa aaaatatgaa tgaaaatagt aggttttata aaaaacatat
     6541 ttagatttta acatctaaaa ggaattttca aacctaa
//
LOCUS       DAEPOG010000190         6556 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEPOG010000190 DAEPOG010000000
VERSION     DAEPOG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.190"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..273)
                     /locus_tag="KR432_003217"
     CDS             complement(<1..273)
                     /locus_tag="KR432_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641728.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKY"
     gene            complement(757..960)
                     /locus_tag="KR432_003218"
     CDS             complement(757..960)
                     /locus_tag="KR432_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641729.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(1011..1262)
                     /locus_tag="KR432_003219"
     CDS             complement(1011..1262)
                     /locus_tag="KR432_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBH0641730.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(1278..2348)
                     /locus_tag="KR432_003220"
     CDS             complement(1278..2348)
                     /locus_tag="KR432_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBH0641731.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(2377..3798)
                     /gene="ccpM"
                     /locus_tag="KR432_003221"
     CDS             complement(2377..3798)
                     /gene="ccpM"
                     /locus_tag="KR432_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBH0641732.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPYERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            complement(3820..5592)
                     /locus_tag="KR432_003222"
     CDS             complement(3820..5592)
                     /locus_tag="KR432_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641733.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            complement(5865..>6556)
                     /locus_tag="KR432_003223"
     CDS             complement(5865..>6556)
                     /locus_tag="KR432_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641734.1"
                     /translation="GKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNVD
                     IIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYS
                     LFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFK
                     NSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLRE
                     KLNSCLVDVII"
BASE COUNT         2305 a         1017 c          541 g         2693 t
ORIGIN      
        1 atattttatt cttataagaa aatataatcc tattactact aactctatca ctaaaaatac
       61 tccataggta agaattgaat caatactatc taacccattt ctaaaaaaaa ttaatgctat
      121 acctgctgct ataatcccta tagcattttt tcttactgca ttttgcccaa ctccataaaa
      181 acctggcatt tttcctttta tttcgacagc agttaaaatt atcccaaaca ctataaataa
      241 tactccaata agcatcataa tcatactaat catctaatta cctccttcac ttaaggaaat
      301 ttcaatttct aaaatctaaa ataaataata tttatttttt ataataatat tgtatatcca
      361 attaactatt ttggaaatta gtaccaataa ataatgttta aatattgaaa taatatatta
      421 attctaatca atattattaa tatatataca tttttacaaa tatatttaaa aactttactt
      481 ttaattctta ctatttaaat aagtataatt tattataata acttttaatt aatttatact
      541 tttaaattgt aaaaatcacc cctaatcact atatttacca ctataataat tgaagaggtg
      601 atttatataa cagatactat ttatgcagtt attataaaat cacactctta attataatca
      661 actgcatatt tttataataa aaggaaataa agtaagaaag ataaatttaa tcaaatatgc
      721 taaatcaatt taatagtata cttattactg aaatatttac attccatcag gagttctttg
      781 accatgtatc aatttattat aatatgtatt atatgatcct cctcttgcat tataatcatt
      841 attcttgcag ttacaagatg aagatgccat catcttacaa gtataaggac acataatagt
      901 acaaacacct cttgtctcaa cacctatatt attcatttct attctttttc caaaactcat
      961 tcatatcctc cccttttcta taagatcact tactttatac ctttattaac ctataccata
     1021 ttgattattt ttagaacatt atcaaaactc gtaaattgcc tattcaaaag tttttcctct
     1081 ggaattttta tattaaagtt ttctattacc tcgcaaaata ctaaaagtaa ttctcttggc
     1141 tgtatatgta aagacggtga aaaaaataat tcttttttta aattgttatt tttattaaaa
     1201 ttaattccag tcacatcaaa aaatatattc tctagtagta attccttttc agtgtaattc
     1261 ataattaacc tccaattcta aatagatatt actttagcat actcttttaa ttgattcact
     1321 atattgtcca ctaattctag taattgttta ttatcatgta taaaaaatgt tttttcataa
     1381 tcttctatat cttcaacaaa aaagttttca tctaatgaca tatatgaaaa tttttgcttc
     1441 acctctgtct catatgtatc tataactttt ttctctatac taaaataatc tatatttata
     1501 ccatgtcttc ctttaataaa atatttaagt tcttcacaat attctctatc atatatactt
     1561 gatggaatac ataatatacc acaatcattt attactgcat tagaaatttc aaaagctcta
     1621 attccaaaat taccaaccat ttttctagag aaagggaata attctcctgg aacttctata
     1681 attattaaat ctggctttgc ctctacctct ttatctttta taaaatggtt aaaattaata
     1741 atctttgtct tactacttat cgaatcatca aacataaact ctggtatagg aatcatttta
     1801 aataaatagc tctctttttt aggaccaaat tgtaatattg aaaaccccct ttttcttagt
     1861 tcttctccta tccttatatt tacatcaaaa gtatctaacc ctccataaag accacaaata
     1921 aatattattg gtgtgtttat ctgtgatata ccctctatat ctcttatttc atatttttct
     1981 aaattccatt cattaatctt tttgaattca taatgagata tatacctaga tattttatta
     2041 agatcatcac gtgtatataa tatcttttta ccttctttta gagctacttt taatttagga
     2101 attataaatt tttctaagtc tagattttct tttgaatcaa caaaccatac caaagaattc
     2161 tttttaagtt cactttgaaa atcatttgta actttagtaa taatattatt aacattatac
     2221 tccttattgc ataaccccca gccagaagga ctaaccagtg aattaatttc tatatctgaa
     2281 attttattgc aatgatttaa aatagtaaaa aaattataat taaatggata tataagtact
     2341 ttttccataa tcccatcttt cctttcatta gaaaatttaa tattgatatc ctagagattt
     2401 caaaacgcag taatctttca tattattttc tgtagaatgt ctaattctgt cacaatcatg
     2461 tggttttatt tcattttcat tacatttagt atcatcaatc ttagctatac atatatcaca
     2521 ataactatag gcccaacaat cagaacattg ctcttcatat aattttccta tatttaaaag
     2581 tttttcagcc ttttctatat ctactccact atctaaattt ccaatcatac ccattttaga
     2641 actttcactt actctctcat atggaaaaaa ttctccatct gtagttacaa atattctaaa
     2701 agcccctggt atacatggtc caccatgatg ccacacatct ggtaactcta atctaccttc
     2761 aatatttttc tcatatctaa aatcatctaa taaggctctt tctattcttg ataaaggtga
     2821 catactctca tcttttataa attttaattt gttgagtaaa gtcaagaatc tatcataact
     2881 cttacctaat atatattcat ctgatgaaac tatttcttta tcagaatagt tattattaag
     2941 agtggaagct aaaaagtttg aatcttttaa agtctcattt ttagatataa aatcatctat
     3001 agaaatgtaa tcattttctg aatttatcac tgtattaaat gtaatattct ttttataata
     3061 ttctggatat ttatttttta tgtaacgtac attttttaat acaacatcat gagatccttc
     3121 tcctgtattt gcaaattttc tacttttatc atgaatttct tttcctccat ctaaactaat
     3181 catagttgtt atattatttt ttacaaagaa ttctattatt tcctcattaa ataaagttgc
     3241 atttgtagtt agattaaagt atatttcctt tccttctcca actactttta catattctac
     3301 tatctgctta atcaaatcaa actcaagtaa gggttctcct ccataaaagc caacactaac
     3361 ttcatttaat tgactagaat tcttaatcaa atagtcaata cttctttttg ctacatctat
     3421 attcattctt ttatttgtat gaactctgtt gctgtatgaa ccagaataaa tgcaatagtc
     3481 acaacgtaaa ttacaatttt gggttacttg taaaataata gaggatagat tattttctat
     3541 atgatatttt atatacttag tttcagggtg cattgtttta tttacccttt tattttttag
     3601 aaatccataa ttaatcaagg tattaatttc gtcttctatt tctatgttcc agtcactttc
     3661 tttttctaaa ttatgctcaa ttttatttag ataattataa acagcagcat ttacctttaa
     3721 aatcttatcc gtattaacat cataaataaa atatgatttt ccagctttaa atgtgtgtat
     3781 aaatggtttt tgattcatta attaacctcc ctttaatatt tatttgcagt tacaaaataa
     3841 attaaaataa acaccctttt tgtccataag ttcatcatgt cttccacttt ctaacaactt
     3901 tccattttca agaactacaa tattgttagt tatctttttt gcatgttgga tatggtgcgt
     3961 tataaatatt ccaatcttat tttcaaatgt ttttttatat aaagaaagga gttcatttcc
     4021 cgcttcagaa tctaaagatg aatctggttc atcaaaaatg tacatatcag catctttcat
     4081 aagagttcta gatattgcta ctttttgcca ttcacccata gaaagttgct tcttaccaaa
     4141 ccaggttcct aaaatagttt ctaatccatc ttcctcataa atacttggtt tcaatttagt
     4201 ttctttaagt ttacttttaa ctttttcttc caagttactc ttatatacat ctcccatggc
     4261 gacattttct tttaatgtag cctcatattt aatataatct tgaaacatac aacttacttt
     4321 tctaatgtat tcatcttttt caatatcctt taaatttttt ccatttatca agatgtcacc
     4381 ttcataatca tcataaaaac ctaataatat ttttaaaagt gtcgttttac ctgaaccatt
     4441 ttttcctagt atagcaatag gatcattttt atttaaagtt aaattaatat tttttaaaga
     4501 atactctgat gaatgattgt atttatatga aatatttatt aactctattt tatttatatc
     4561 ccctattttt tcaaatattt ttatatgttc aattgagtct tctttacagt tataatctag
     4621 aaatttaaac agtaaatctg tataaaaaag atcttgtatt actgaactaa ataacgaaaa
     4681 tattgattca acattatcct ttatagattg aactgcttgt atatatgtag tcgcatctcc
     4741 tattaaaatc ttctttaaaa taccttgaat tattataaat cctataattc ctgcagatac
     4801 aattgaatct aaaactgtac ataatagcgt agcaataaaa ttctttttag ctatattcat
     4861 atcttgagat ataatatcct cttttaattt tttataatga ttaataagtt tagaaccaat
     4921 tctaaatacc ataatctctt tgtaagcata accttttgac aacaaataat ctatgtacca
     4981 ggcctttctt tctttccaag ttctgtttac actaacatca tattgttcct ttcctaattt
     5041 aatggaatat aatgatttca atacaggaat aacaataaca actattatta gccaaagttg
     5101 aaattgtgat aatacaacaa taagagaaag tgtagatact gattctctta ttattgaaat
     5161 aaggttttca gatagtatag taatattaaa tcctttttga gattgagctc tatttatcat
     5221 gtcataagta ttatcatcct caaaatcttt taaactcaaa gttaaagatt tctctaacat
     5281 cattgtttca atatgttttt caaaattttt attaaacttt attctataat atccataaaa
     5341 actttgaaaa attgaaggaa ttatatttat gattaaatac ataattaaca aaactaatat
     5401 ttcttttaca cttttcgaaa atgtttgcat attattaatg atattttgac ttactaacaa
     5461 agaaatagat ggaacaatcc cctgaaaaat tgccataaac atagagataa ataaatatct
     5521 tttatcatgt agataaatca tattaaaaca atttcttaca cctttaaaaa attgaaattt
     5581 accattttcc acaatacacc tccatactta ataaaattga aataaaatat tttattaatt
     5641 aatataatat aacattattt tattatgtat aatacatatt taaattttaa attaaatctt
     5701 taaaataaaa ttgttaatag aattgtaaaa taaaatctta atatattcaa tctgttattg
     5761 atgaaaggta tatttttaac tataattagt ttttattata aattttttca tattttacaa
     5821 tagatatatt ctattagaat agaatataag ggcaaaaatt tacctcaaat tattacatct
     5881 accaaacaac tatttaactt ttctctcaat aattttatct tataacgact tacaattatt
     5941 gaattttgat ttataaatac aatattattt tctatatcta gcatatttac cttcattaaa
     6001 tttataaggt agcttttatg acatctaaaa aaattatgtt cttctagatt tgattctatt
     6061 tctttcatac tattcttaaa agtatatatt cctttttcag tatgtatagt taaatcattt
     6121 tttaaaactt ctaaatataa aatatcatct acctttatga aatatatctt gcatttacat
     6181 tttatagata aaatagcttc atttttgtag cattctttta aatatgatga tattgcttct
     6241 ttaaatagag agtattttaa gggcttctct aaatatctat aaactcctat ctcataacct
     6301 ttctttatgt atttatttga tgaggataca aatataattc ttacactttc atcaaatttt
     6361 ttgatttctt ttgaaacatt aaatccattt tcatcattta agtctatatc cataaatatg
     6421 atatcaacat tattaggata cttttttaat aaatcgcctc cattttcaaa ctctttgata
     6481 ttgtattcaa aattgtaatt ataaaaaaag gtatttatat aatcatttag aattaccctc
     6541 tctgattttt tacctt
//
LOCUS       DAEPOG010000191         6544 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEPOG010000191 DAEPOG010000000
VERSION     DAEPOG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.191"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..117
                     /locus_tag="KR432_003224"
     CDS             <1..117
                     /locus_tag="KR432_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0641735.1"
                     /translation="RAKELGLDAIHDTVHEMCKDEARHGKAFLGLLNRHFGK"
     gene            208..1356
                     /locus_tag="KR432_003225"
     CDS             208..1356
                     /locus_tag="KR432_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0641736.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            1409..2674
                     /locus_tag="KR432_003226"
     CDS             1409..2674
                     /locus_tag="KR432_003226"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH0641737.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(2960..4192)
                     /locus_tag="KR432_003227"
     CDS             complement(2960..4192)
                     /locus_tag="KR432_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641738.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(4195..4869)
                     /locus_tag="KR432_003228"
     CDS             complement(4195..4869)
                     /locus_tag="KR432_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641739.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(4877..6106)
                     /locus_tag="KR432_003229"
     CDS             complement(4877..6106)
                     /locus_tag="KR432_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0641740.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(6218..>6544)
                     /locus_tag="KR432_003230"
     CDS             complement(6218..>6544)
                     /locus_tag="KR432_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0641741.1"
                     /translation="WLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCG
                     IKKEDLNRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTI
                     TFKCDL"
BASE COUNT         2315 a          914 c         1043 g         2272 t
ORIGIN      
        1 agagctaaag aattaggatt agatgctata catgatacag tacatgaaat gtgtaaagac
       61 gaagctagac atggtaaagc attcttagga ttattaaaca gacattttgg aaaataataa
      121 ttaaaacata gattaaaagg agaaactttg gtttctcctt ttttaaatta tacaaataaa
      181 ctgttgtgat agaaggatgg agataatatg aagtcggtat ctgttaggag ttgtaaaact
      241 tacaattatg aagaagtaaa gttatctatt gaaaaaaatt tagaggatat tggtggtata
      301 gataagtttg ttaaaaaagg aagcaaagta cttataaaac caaatttatt aatgaaaaaa
      361 aagcctgaag aagctacaac tacacatcct atggttgtaa aagttgtatg tgaaaaactt
      421 ttagagttaa attgtgatat tacaatagca gatagccctg gtggtccata taatcaaaat
      481 tctcttaaga gcatttataa ggcatctgga attgaagaag tagcaaatga actgggcatt
      541 aagttaaatt atgatgtttc agatgttaag atacatagta aaaaagcaca tgttctaaaa
      601 tatatggata ttattaaacc aatagtagat tcagattata taataaatct ttgtaaatta
      661 aagactcata taatggcaac ctttactggt ggtacaaaaa acttatatgg atgcatagca
      721 ggattgaaaa aagcagagat acattataga tttcctacag aagagttgtt ttgcgaaaat
      781 gtacttttag atatttgtga ttatataaaa ccaacattga caattatgga tggtatagta
      841 ggtatggaag gtgatggacc ttcagctggt actactcgag aaataggagt tttattagca
      901 tcacaaaatc catatgctat agatgttgtt gcatgtaaga ttataagttt acttccaaat
      961 agagttgcta caattagagg tgctattaag agaggctata tacaagaaga tttttcagat
     1021 attgaaattt taggtgaaga tataaaagat ttaattataa aaaattacaa gattccaaat
     1081 gtaagtaagg atttaagatt gttttccgta aagctaccaa aatttttaaa tgaacctatt
     1141 tcaaagctta taacaccaaa gcctgtggta agatttcaag attgcataaa atgtggaaaa
     1201 tgcaaggaag cttgtcctgc atccactata gagataggga ctaaaggtgc tattatagac
     1261 ttaagtaaat gtataagatg ttactgttgc catgaacttt gtcctaaaaa ggctattgat
     1321 gtcaaacgta attttgtatt taagtatgta aaataatgta taatattata tgagtctata
     1381 taaattaaaa atataaagga gaaataatat gattttaatt aaaaatggta gagtaataga
     1441 ccctaaaagt caaagagatg agaatattga cttaattata aaagaaaata aaatttataa
     1501 aataggcaag tttgatgaaa gtgatgaata tgaaaaaata atagatgcta gtggtaatgt
     1561 agttgctcca ggtttagtgg atgtacatgt acactttaga gaccctgggt ttacatataa
     1621 agaagatata gaaagtggag ctaaatctgc tgctagaggt ggatttacaa ctgttatatg
     1681 tatggcaaat actaatccta ttgtagataa tgaagatact tttaattatg ttaaagaaaa
     1741 gtcaaaaaat gcatgtataa atgttttaca agcagcagct ataactaaag gttttgaagg
     1801 taaagagtta gttgatatgg aagctcttaa aaaagctggt gtaccagggt ttactgatga
     1861 tggattacct ttaatggata gcaatctaat tatggaggca atgattaagg caaaagagtt
     1921 ggatgtacca cttagttttc atgaagaaga cccttcttta gttggaaatc caggtgtaaa
     1981 tgcaggaaag gtagcttcag aactaggatt aaaaggtgca tcaagtgtag cagaagatgt
     2041 tatggtagcc agagattgta tgttggcact aaagactggt gctaaagttg atatacaaca
     2101 tataagttca ggtgtatctg ttgatatggt taggtttgca aaatacttag gtgcaaatgt
     2161 agttgcagaa gcttcaccac atcactttac attgactgag gaagatgtat tggagtttgg
     2221 aacaaatgca aaaatgaatc cacctctaag aagtaaatgg gacagagata agattataga
     2281 gggattaaat gatggtacta ttgaaataat agcaacggac catgcacctc atagtaaaga
     2341 agaaaaagat agagagttta taaaagctcc aagtggaatt attgggcttg aaacttcttt
     2401 agctcttgga ataacaaatt tggtgcataa aaatcatctt agtatgatgc agttaataga
     2461 aaaaatgtct ataaatcctg caaagttgta caatttaaat attggattta tagaagaagg
     2521 ggctgtagca gacattgtta tattcaatcc agaagaagaa tggacagttg aaagctttgt
     2581 ttctaaagct gataattcac cgtttaaagg taaatcttta tatggaaagg ttaattatac
     2641 aatatgtaat ggggagattg tatataacgc ctaatttaag attatttttt taaggcggag
     2701 tgtacaatga ttgaaagtaa aagattgtat atcagcagaa tgagattgat taatcaaaat
     2761 tggaagtatt agtttgaaca attagaacat aacatcttgg taatttagat atagtaagga
     2821 agagaatcct ttttatgaaa gaacgcacta ttatatttgg aaagattaag ttcctaatct
     2881 atcatatata atagtgcgtt atatatttaa gtatgtttta tataaactaa tctaaaaatc
     2941 gaaaatcttt atacaatttc tacttataaa ttgcctttat agggtctagt ttagaagctt
     3001 taaaggctgg aatcagacca aatacaactc cagtcaatat agtagctaca attgcataaa
     3061 ataagctatt taagctaggt atagcttcaa aacctatata tttagaaaca tagttagtag
     3121 cagcaaatcc aactatagtt cctaaaattc caccacatac tgttataaaa acagcttcaa
     3181 ctaaaaattg aaataatata gacctaggtt ttgctccaat agcacgtctt ataccaattt
     3241 ctctttgtct ttccataaca gatacataca ttatattcat tacaccaata ccaccaacaa
     3301 acattgcaac tacagttatt atagatacat atttgtttac attggaatta atgctttcaa
     3361 gataagctgt ctgttcagtt gggtctggtg tagtataaga accattgata ccagggtgca
     3421 tctcatataa tttagctata acattgtttg caacttcatt tacattatag cctttagaag
     3481 aaactaagtc taactggtaa atttcatttg aataagaatt ttgactcatt aaagtgtcaa
     3541 aagccttttt aggtactagg gaagtagtaa attgcatatc ttggtaacct ccaaagaatc
     3601 ctccagcttg gttttgctga gaatcatcta atacccctat tatttcaaat atagttccat
     3661 ttatgttgat accatgacct aaggcatctt ctggattttc aaaaatttca cttgtacttt
     3721 gtaatgtaag taaaattact tttctttttt catcatctaa agaaaaatct ctaccacaaa
     3781 ttaaatttat tttagaatct ttttttactg gtccaacatc aacatacgta gtttttttat
     3841 caaaagatgc ttgtgaagag taaactgaat caaggttaaa cccatctctt gaaggagcta
     3901 ttctttctac tccttcaaca aatgataatt cttctaaatc ttttgcatta aagggtttta
     3961 aaaaaatact catatcatca gtaagacctg tgttatcggc tgattcaaac gaaatagtag
     4021 ttttattagg gtttacattg tttacagatt tcttaatttc tttttgaaat ccattaccta
     4081 tagaactaac aagtattact gaagttattc ctattataat ccagagtaaa gctacaaata
     4141 cacgaagctt gtgacctttt aaattggcca aagaattttt taataaactc atgattacac
     4201 ctccgaagta aacacaccat ctttaagtcg tataacctta gttgcatact tagttaaatc
     4261 ttgatcatgt gtaaccatta ttatagtctt accttgtttg ttgagccttg ttaaaagctc
     4321 cataatttct ctgctagttt cactatctaa tgccccagtc ggttcatcag caaagattat
     4381 ctgagggtca tttactaggc aacgtgcaat ggctacacgc tgttgttgac caccagataa
     4441 ttgaagtggt ttctgcttaa gcttgtccaa aagcccaaca gaacttaatt tatctttaac
     4501 aatttcttct ctattagatt tttttagtgc tttattgtaa attagaggca actctacatt
     4561 ttgatataca tttagagttt ctattaaatt aaattgttgg aaaacaaatc caatattaat
     4621 atttctaaaa acagaacgtt cattttcact caaattagtt acatctgttc catcaaatat
     4681 atatcttcct tcatcaaata catctagaaa acctaagata ttaagtaaag tagttttacc
     4741 actaccagac ttacccatta tcatgacaaa ttctccagat tcaatttcta aatttaagga
     4801 ttttaaaaca tgtaattcat ctttccctac tttgtaatat ttttgaatat tttctaattt
     4861 aattaacata tgtaaattaa ttcacatcca tagtaacttc ttctactttt ttgtttggag
     4921 tagtatctcc attattagaa ccagcagtta cgccatcgcc ttgaactggg tctccttctt
     4981 tcatttcttt agtagggtgt tttattatta tatcgccttg ttcaagaccg cctctaacta
     5041 cagcaaaatc atcattttga cttgctacat ctacaatttg ctttttcaat ataccatcta
     5101 aatccttaaa tacatatgag tgtttgttgt ctttaagtac acaagatgct ggtattttaa
     5161 agcttgaatt agctacttca agtgatgctt gaagatggaa tccatttact aatccttctt
     5221 gattatcaaa tgctatgttt atatcataat aagatagtgt attctgctga gctcccattt
     5281 cagtatttgg agtagatggt ttatcagata taaaacttat tctaccagta agcttttggt
     5341 cagttgaaaa aactagtata ttgaccattt ggtcaatctt cattttaggt aaatcttgtt
     5401 cacttgcttg tcctttcata taaaactcta gaccttgaat agtcataaat gaaactggtt
     5461 ggtctggatt tccagtttgg tcattcaagt atactgtgcc atcaaaagga gcagtaactc
     5521 tgtcataagc ttttttatcc agagaagcta tctgagagtt taaagcagtt atctgtgcat
     5581 ttatagaagc aatagcatct ttattttcag caatatcaga aagtgatatt ttttgttttt
     5641 tgtattgact aagttgtgat tttaaactat ctatttctgc tattatagtt ggatttttag
     5701 cagtaaaaag aaggtctcct tgatttactt tttgaccatt tgtaacatta acatcactta
     5761 actcataatc tccagatata ttgaagtcct tagtttgctt aggaacaacc atcccattta
     5821 taaatatttt ttcattttca ggaatagtat atgtttcgat aaaattttca ttagcttgta
     5881 attcagtgtt tttcgaatca acataagcaa gaactcctag aattaaaagt atgattactg
     5941 ctattcctgc atatattttg aatctcttat ttttaaaggg attgttttta aaacctttat
     6001 ttttagactt gaagccacta cttccaaaac tgctatttct aaagttgtta cttttaaaac
     6061 tgttatttct aaatctttta tttttaaatt tttttagaaa agacatgtct tcctcctttt
     6121 tataaaaact acttaattat atactgggat gtacatatag aattactata agaagttgaa
     6181 tataagttaa tgaaaataaa ctatcaataa taagatacta taaatcacat ttgaatgtaa
     6241 tagtaaattt tgtatattgg ttgtactctg aatctacatc aattttatga ccaagcttat
     6301 cacaaagttc ttttactaaa taaagcccaa gacctgtaga tttagaattg tttcttccat
     6361 tattacctgt aaatccctta ttgaatattc gatttaaatc ttcctttttt ataccacaac
     6421 cagtatcttc cactcttaat tgtaaataat ttttggttct tttgcaatag atagatatat
     6481 tatctcctaa tgaggagtat tttagggaat tactcaataa ttgctcgaat atgaagtata
     6541 gcca
//
LOCUS       DAEPOG010000192         6496 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEPOG010000192 DAEPOG010000000
VERSION     DAEPOG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.192"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..386
                     /locus_tag="KR432_003231"
     CDS             78..386
                     /locus_tag="KR432_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641742.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(548..2338)
                     /locus_tag="KR432_003232"
     CDS             complement(548..2338)
                     /locus_tag="KR432_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641743.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2338..4065)
                     /locus_tag="KR432_003233"
     CDS             complement(2338..4065)
                     /locus_tag="KR432_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641744.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4310..4874)
                     /locus_tag="KR432_003234"
                     /pseudo
     CDS             complement(4310..4874)
                     /locus_tag="KR432_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            5163..6275
                     /locus_tag="KR432_003235"
     CDS             5163..6275
                     /locus_tag="KR432_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0641745.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
BASE COUNT         2225 a         1037 c          839 g         2395 t
ORIGIN      
        1 atatttatgt tctttgtaaa attttttaga agcttctaat gttatgctat aaaactatct
       61 tgagaagggc tgattgaatg tttaataaaa tatatgaaaa gaaagaattt atagtttttc
      121 aagttaaaaa aggttatgta gtttataata ctagaaagag ttttgaagaa ggtcatacac
      181 atttaaagca ttttgaagca gcaaagacag ctattgattt agcaataaat aaaaagattc
      241 ctagaagcaa agatggatat tatctgacta gtttaattcg cataagtgat gatggttatt
      301 acattgataa gttaagcgaa ttgttatatg ttagagaaca aaaaggtaaa aaagaaaagt
      361 actgtaattc tggttatcag atgtaacaag actagttgtg aattataagt accaatcata
      421 aataccttta gtcttaaaat caaagagtat cagtaaatct ataaaaaaac aacttactga
      481 tactcttttt aattatatga ttaatatttg aatttgatac aaaattttat acaaaagttt
      541 atgttatcta ttctgattct ttgtctgcaa attggctatt ataaagcttc tcataaaatc
      601 cacccttttc cattaattca tcatgattcc cctgctctat aatacttcca ttattcataa
      661 ctaaaatcaa gtcagcattg cgaattgtag aaagtctatg tgctataaca aaactagttc
      721 taccattcat aagatttctc attgcttttt gaagcataag ttccagccta gtatcaacag
      781 aactagtagc ttcatcaagt atcaatattg aagggtctga tataaaagct ctagctatgg
      841 ttaaaagctg tttttcccct agagatatat tagatgcttc ttcatttaag aacatattgt
      901 atccatctgg aagagtggtt ataaagtgat gaacatttgc tacttttgct gcttgtataa
      961 tttcttcttt agtagctcca aatctaccat attctatatt ttcaaaaatg gttccattaa
     1021 ataaccatgt atcttgaagc accataccaa acattgaacg caaatcttta cgtttcatat
     1081 ctcttatatc tactccatca attttaatag cacctttctt tacatcataa aacctcatca
     1141 ataagtttat taatgttgtt ttacctgctc cagtaggtcc aacaatagct accatctgac
     1201 cactttttac ttctgcattt aaatcttcta ttaaagttga attttctcca tatccaaaat
     1261 taacatgctc aaatgtaaca tttcctttta cattttctat cttaactgga ttttcaacat
     1321 caggtatttc ctcttcttcg tctaaaattt caaatacacg ttctatagct gcaaaagaag
     1381 attgtattac tgtagataac tgagtcattt gtgaaagtgg ctgatttatc tgccatatat
     1441 aacgcacaaa tgcttgtaaa tttcctactg ttatccctcc acttactgca tatatagaac
     1501 cgacaactgc cacagctgca attccaaagt aagttactaa tgaaaccata ggattcatca
     1561 tacttgatat aaattgtgcc ttaaatccat tttcacaaag ttcttgattt acttttttaa
     1621 attcttttat agaatcttct tctttaccat atagttttat ttcattaaat ccagtataca
     1681 tttcttgtac ttttccattt aattttccaa gagcattttg ttgcaatgaa aatctacttt
     1741 gagatttttt tactacaaat ttagatacaa aataactaat tggaattata aaaagagcta
     1801 taagagccat atatatatta attgtaaaca tcattgaaag tgccaaaatt atgactaaga
     1861 ttgagtttac aatttgcata aaactctgtt gcaatgcatt tgatattgta tctacatcat
     1921 ttgatatacg acttaataca tctccaaaac tattactatc aaaataacta ataggcaatc
     1981 ttcttatttt attttcgact tcatttctta aatctctcat tgtgttttga atagcattcg
     2041 taagtagaaa acttgaagta tacgtaaaaa ttgcattagc aaaatatata catgctaaaa
     2101 tcaccaatat ttttgttaca taatcaaaat ttacggaagc ccctggtact ccttttgcga
     2161 tactaattac atctttagtc aattgtgtta ttatcaatcc ttcagtttta ggtgcaagtg
     2221 cattcatagt agctgctgaa attatacata taatagctat aataaaggaa aatttatatt
     2281 tctttacaaa tggaagaagt ctaccaattg ttttcttaaa acctttagac tttttcatta
     2341 ttctaattcc tcctttttta actgagatgt tgcaatttca tagtatattt cacagttttt
     2401 taataattct ttatgagttc ccattccgac aacttgtcct tcattgagta ctataatctt
     2461 atcagcatca ataatagaac taattctctg ggcaacaatc aatacaattg catcttttgt
     2521 ttccttcttc aatttagcac gaagtattgc atccgtttta aagtcaagtg cagaaaaact
     2581 atcatcaaaa atgtatattt caggtcttct tataatagcc cttgcaatag aaagtctttg
     2641 tttttgacct cctgatacat ttgctccacc ttcactaatc aattcatcaa actttctagg
     2701 tttttttgat ataaactcat atgcttgtgc aactcttgcg gaatattcta attctgactc
     2761 tcctgcctta tgtttcccaa atctaatatt atttgctatg ctaccagtaa aaagcagtga
     2821 tttttgaggt atgaatccta tcttttttct taaagctttt aaatcatatt ctcttatatc
     2881 tactccattt atcttaatgc taccctctgt tacatcgtaa aatctaggta tcaagttaat
     2941 tagagtactt ttcccactac cagtacttcc tataaaagcg actgtttcac ctgtttttgc
     3001 agtaaatgaa atatccttaa gtacagaagc ttctccatct ggataggtaa aagttacatt
     3061 atcaaattct atgataccct tattttcagt atccttaatt ccattttcag gattttttat
     3121 caatggttct tcatttaaaa gttcttttat tctatttgca gatacctctg ctcttggata
     3181 cataataaat accattgaaa aaagcataat tgaaaacatg gcatggaaaa gatattccaa
     3241 aaatgcaacc aactgtccaa cttgaagctc tccaatattt atcttctcac ttgaaatcca
     3301 aaatacagcc aaaacagcta tatttaatag taaaaagaat gctggttgag taattgacat
     3361 aagcttaaat agtttcttag aaacattagc ataatatgta tttgtttttt caaatcgctc
     3421 tgtttcataa tcatcatttc caaaagctct aattactctt attccagtta aattttctct
     3481 cgatataagg tttaatttat ctagtccttt ttgttgtttt gaagatattg cctgagatac
     3541 ttttgctatt attgctactc caataattat aaaaggtact gatatagcca atactataga
     3601 aagttccaca cttgttctaa ttgtcattat tatacttatg atgaacataa ctggagttaa
     3661 taaggcagtc cttagtagta tattaacaaa ttgttgaatt tgaaatgcat cattcgttgt
     3721 tcttgtaatc attgaagaaa ttccaaattt attgtattca gtatgtgaaa attcttgtga
     3781 tttctcaaat atgtcatttc ttatatctct ggttacactt gttgatacct tagcagaaca
     3841 ataacccaac aatatactac ctacaacccc tataatagaa ataataacaa tcactattcc
     3901 catttgtttt atgtaagcta tattctgatt tgcaatacca ttgtcaatta ttttagctat
     3961 tatagttgga atccccaact cgactaatgc aaaactaaat atagccagta tgtttaatac
     4021 tattaataac ttataattct ttaaataact taaaattaat ttcatatttt tctcctttct
     4081 taatttttga gatagtgtaa tcatgtaata atactaaatt tataaatgct taattttata
     4141 aaaaaaggag aaaagagtta acgcactctc ttctccagca tattcttttt aatacaccct
     4201 ccgacaagtc gcttatatgt taacatattt gacaagtaat gtcaattcat atagctaaaa
     4261 gtcttttaaa tgcttcatct cataacattg taacaaattg gcatgtaatt tatttttgtt
     4321 ttttactgta ttccaatttt acaagttctc caatattact aactttctta aaattcaaat
     4381 tagtcttatc tataccagac ttaaatagtg aaatccctct ccccaatatt attggcaata
     4441 tatatatata ataatcatca attaagttat ctttcataaa taattttact atatcgcctc
     4501 caccaactat ccaaatatct tttttatcct tcttttttcc aatttgctca attaattttt
     4561 taggttctaa attagtatac tttacattct ccagttgtcc cattaattgt atttgagtat
     4621 atatagcatt ccttaccctt atagtaccat tcatttgggc ttaattcatt tgcaatctgt
     4681 acatatgtgg aatttcccat aatgatagta tcaattgtat cgtaaaaagt attataatca
     4741 tcttcctgct cattcttaaa ttctgaagat atctcgtcta aaaaacttac actgccattc
     4801 tcttcagcta tataaccatc taaacttgtg gcaatataaa gtactacttt tctcatgttt
     4861 actacctcca acatttatag tgtgattatt ctatgtaaac atcacttttt tactcattca
     4921 ataataccct attaataaat cctcaaacta atttaaataa actttgcata aaactaaaaa
     4981 tatattatat aaataattta aaatcacact aacttattga aaattctaat tattacaaat
     5041 aaatattgct ctctgttaac gctagtttta taatataatt ataagctatt aatattataa
     5101 agccgatatt actaatataa taattatagt taatttactt ttgttgaaag gtggtaaata
     5161 acatgaaaat ttcagaagtc gctactccaa attttttgct agatttagac cagttagaaa
     5221 agaatataga taaaattcaa caaatttgta caaataataa taaacaactt tggcctatgc
     5281 ttaagacaca taaaagtact tacatagcga gacttcaaaa aaatgcaggt gctagtggtt
     5341 ttctagttgg aacacttgat gaaggtgaag ctctgataaa agcaggtttt gaagatatta
     5401 tgttggctta tccttacatg ggagaagtaa atataaatag aattgtgact atggcatcaa
     5461 aaataaattt aatctgtgct acagataata tagaatgtgc taaggcttat agtgaagctt
     5521 tttctaaatc aaaaatagag tgcaagctac ttattattgt agattgtggc ttacaccgtt
     5581 ttggggttga accagaaaaa gtggttgaac ttgctactga aatttctaag ctagaaaaca
     5641 tcaagattgt tggtataagt tcgcatccag gtcaagtgta cggttgtagt tctccagaag
     5701 gtgttccaga tgtttgtgtt caagaagatt cttctatgga gaaagcatac aaagctctaa
     5761 ctgataatgg atttaatata gaaattgtcg cttctggtag tactccaaca gttgaagctg
     5821 aagctgcttc taaaactata actgctgtaa gacctggaaa ctatgtatac tatgatgcta
     5881 tacagatagg tttaggttgt gctactgaag atatgtgtgc tcttactgtt gtgtgtactg
     5941 taatctcaaa aaatagcagt ggatattatt taattgattg tggaagtaag tgccttggac
     6001 ttgataaagg tgcacatgga aatagtagta ttgttggtta tggtagggta tgtggacatc
     6061 cagaacttac tattgattca ctttcagaag aagttggaaa aattaaagta aatggtgaaa
     6121 ctgatataaa aattggtgat agaataagaa ttatacctaa tcattcttgt tccactgcaa
     6181 acttgacgag taatttatta ggttttagag gagatacaat agaaaaagtc atagaagtag
     6241 atattcgtga aaattcaaaa caaatcatcc tttaaaaata tatttatttt gatattaaca
     6301 tcatgaaagt atatttttct taaaattatt gttaataact aatgttaatg ttggataaat
     6361 aaaaaacata aattctagaa atataaattt aaaaaaagag gctgtctcaa aattggatat
     6421 gtccctgtca agtagacaga cttaaaaaag gctatcttta tgcggaatga gttcgatatt
     6481 caatcggact cattcc
//
LOCUS       DAEPOG010000193         6392 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEPOG010000193 DAEPOG010000000
VERSION     DAEPOG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.193"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            411..1685
                     /locus_tag="KR432_003236"
     CDS             411..1685
                     /locus_tag="KR432_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0641746.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1892..2209)
                     /gene="trxA"
                     /locus_tag="KR432_003237"
     CDS             complement(1892..2209)
                     /gene="trxA"
                     /locus_tag="KR432_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0641747.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2476..3228
                     /locus_tag="KR432_003238"
     CDS             2476..3228
                     /locus_tag="KR432_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBH0641748.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3215..4096
                     /locus_tag="KR432_003239"
     CDS             3215..4096
                     /locus_tag="KR432_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0641749.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4322..5677)
                     /locus_tag="KR432_003240"
     CDS             complement(4322..5677)
                     /locus_tag="KR432_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0641750.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2451 a          930 c          805 g         2206 t
ORIGIN      
        1 tataacatat aaaatctttt tttctaatta tatacaatat attttccatt atgttctaat
       61 caatttcttt actattttta caactttatt agtcatattc ataattattt ctaattagtg
      121 tttaacattt tatgtaaaat ttataaaaaa atgtatataa attaaaataa gtgactatac
      181 taaaaatatg atacggataa acaataagtt caacctatct cccccaaaat caagtgagct
      241 acagtttccc ccaactgtag ctcattttcg tgtaatttaa taaacatctc aacaagaatt
      301 aattaacaaa accccttata aaaacataac atatattaaa caagctcttg attaaaactt
      361 gagctttttt aataaaaaac agaatagttt attagataag gggttttttt atgcaattca
      421 tggatgtatc taaatacatt gaaggtgcaa accttaaagt taaaactaaa aatggtgaaa
      481 ctgttaaaag aatatatttt aacaactctg ccactccatt agttttaaaa aacgtagtgg
      541 ataacttaaa ctgtgagatt ccttggctta cttatattaa tgctccagga ataatctcag
      601 aaaaaaatac tttaaaatat gaaaatgtac gctctactat cctcaaactt attgatggag
      661 acgaagaaaa ggattctgct atttatgtca aaaatgctac agaaggaata aacttcttag
      721 caaaattttt taaagatgaa aatccagata aatttattat taccacagca atggagcata
      781 tggctaatta cctacctttt aaagttaatt ttcctacaaa agttgttgga attacagaaa
      841 aaggagaact tgatttatgt caattagaga atatacttaa aaattatgct ggtaatgtat
      901 ctctcgttac agttacagga gctagtaatg tcacaggtat aaccactcct atatatgaaa
      961 tagcaagaat agctcataag tatggtgcaa agatattggt ggatattgtg caagtaattc
     1021 agcataaacc tttttcaatg atgccacatg aatgtgatga gcatatagat tttgttgttt
     1081 ttagtgctca taaatgttat tctccactaa atggaggagc tctggtagga cctaaaaaat
     1141 tctttgaaaa atttaatcct gtactatatg gctcaggttc taccaaattt gtaaatgata
     1201 acaaaatact atatgcaaac gtaccccaac gttttgaagc aggataccca gagtattttg
     1261 gaactcttgc aatgggaaaa gcactgaaca ctttaaataa aattggtcta aacagaattt
     1321 caaaatacga aagagaatta tttttatatg ctaaagaaga aatgagtaaa atacctaata
     1381 taataatata tggagataaa tcaaataatg taattatccc aaatatatct tttaatataa
     1441 gtaatatgta ttacaaagat gtagcaaaat atttagttga taactttgga atagaaactg
     1501 gagctggagt agtaggtgca gatatttatg tacaaaaatt attaggtata agtccaaaag
     1561 aagcatacgt cagatttatg agggaaaatc ctgtaggtct tgttaggata agcttaggaa
     1621 tgtacaatac ttttgatgag gtggatagct ttatacatgc tattaaaact ttggctttta
     1681 aataagcttt ttctattctt ttttatcaat aaatagagag tagttttgtt ttcacaaaaa
     1741 aacaaattat atcaacagtc aatttcatta gcaattagac actcatttgt aactatacaa
     1801 actattttac actatacaaa taaaaaactc attacaatga gtttttttgt ttatatatga
     1861 tatttatttt aattacttca atttacttta attacatata tgattcaatt ttgtttttta
     1921 aattctcctt aggcataaaa cctattaatc tatctacagg ttttccatcc ttaaatatta
     1981 gcatagttgg tactgtagat acttcaaatt tctgagctaa ttctatactt tggtctatat
     2041 ccaccttcaa gaagtttgct ttttcaacca tctcatttcc taaagcttca tatattggtg
     2101 atagcatctt acaaggtcca caccaagtcg caaagaaatc tacaactact aaaccatctt
     2161 tattttctac ttcatttata aattcattat tatttattat ttttaccata atatacatct
     2221 cctttattat cttcttgata ctaatatact acgccctata taacagttca attaggcact
     2281 tttttgtaac cgcctatttt taatagattt aatattatga taaatagatt tataagatta
     2341 aatatttata agactaaata tttctgatat attttatttt tttcttaata tagttgacat
     2401 tttctccctt aaattataaa atcaaattaa taactacata ggtagttact aatcttttat
     2461 caaaggagtt tctatatgga taatataatt aatgaattac aaaatatagg atttactaaa
     2521 tacgaatctc aaatatacat atcattatta aaggaaagcc ctttaacagg atatgaaata
     2581 agtaaattat caggtgtacc acaatcaaaa gtctatgaaa atataacaaa gctattaaac
     2641 aataatataa ttatcaatgt tggaactgac cctattaagt acatacccat aaaccctaat
     2701 gaattattaa aaaataagaa aaaagaattt gataattcta tggataatct aaagaatagc
     2761 ttacagatat taaataaaaa taaaagtgta gaatacgttt taaacataaa aggagataaa
     2821 aatatattaa aaaaagcatt agaaatgata aaaaatgcaa aacaagaaat tataatttct
     2881 tgcccttatg aagaaattct tgaattaaaa aaagaactcc taattgctta caaaaaaaat
     2941 attaatattg aaatactact tttagaaaac aaaagtctga aaggtcttga taatattcat
     3001 actcatggag gtaataaaac tacacttgaa aataaaatgc tttacaaagg aattattttg
     3061 attgtagata atgatgaaat attgacagga aacttatcag aaggcagtga ggccatttca
     3121 atttggacaa aaaactctaa cattttatat attggagttc aatacataaa acatgaaatt
     3181 tatatttcag aacaaataaa aggaggttat aaaaatgaac aaaattaaga ttaaacaagt
     3241 tgatgctttt acaactatac cttttggtgg aaatcctgct ggtgtcgtta ctgatgcctc
     3301 taacatttct gatgaaatta agcagaatgt ggctaaagaa atgaatttat cagaaactgc
     3361 atttgtttca gagtcaaatg ttgcagattt taaggtacaa tttttcacac caggtttaga
     3421 agttgattta tgtggtcatg caacaatagg tactttccat gcacttttgg aagaaggaag
     3481 gcttaatact aataagagta tattttatca agaaacaaaa gctggtgtat tatctgtaga
     3541 gcttaaggaa ataaataatg aaaatatatt tatgatggaa cagaatacac ctgaatttga
     3601 aaatttagat aattacagaa aagaacttgc acaaatgata ggtgtgcatg aagatagact
     3661 tcttgaatat cctattataa aggtaaatac tggactttgg tggcttgtat ttggtctaaa
     3721 atctttagat gatttaaaaa atatctctcc agatttaaat aaaatcaaaa aatttagtgg
     3781 tgataataat ataattggta taactccatt ttgtatagaa actatagata aaaagtgtga
     3841 ctaccattta agatctatag caccatatgt tggagttaca gaagaccctg tatgtggaac
     3901 tgggaacgct tgcgtatcat cttatatagt acatcacaat attttagata aaactagttt
     3961 tattggtgag caaggtaact ttataaacag acctggaaag gtatatgtaa atataactag
     4021 agaaaacaat gacataactt ctgtaaaaat tggaggaagt gcttatactg ttttaagtgg
     4081 tgaaattatg tattaatccc atacctaata aaaacaaata aaaatactag ctaactaaag
     4141 ctagtatttt tatttgttat ttttatttaa taatcacact ctaacaaata ttataattat
     4201 atatatcata atattgtatt tagaagtaaa attttatttt tattattaat tagttataaa
     4261 tcaatctaat acaacataat tacagtagat taatctataa aacataataa ttacttactg
     4321 attatttatc atcctcaaca agtctactta atgtcttatc agataagaat aataatatcg
     4381 ctgcaacaaa tagtattact ggaattatag tatatacttt aatcatatca aatttaagag
     4441 taaatgcata tatatatcca tatcctttac cagctataaa agtagcaaat gtccatactc
     4501 ccataagaac acctagaagc ttcttaggtg catacttact tacaaaagag ttaccaagtg
     4561 gtgagaataa catttcacca agacttaaaa gaataccaaa catgattatc catagtatac
     4621 tagctttagc agtttcagga gctccaactc ctcttgaaaa ttctgcacca acaagcatta
     4681 agaatgcaag acctaagaat attaatccaa gacctgtctt tttaaataaa ctcatatctc
     4741 cttgtggtct actagcaagt ttaaaccata atgctccaag aactggtcct aatactatac
     4801 atacaagtga atttaatgag tcaaaccaag caagaggaac atcaaatcct ccaacattca
     4861 tgtttacaaa tgctgcacca taatcataaa ctgcaagata agttagatac cagaataccc
     4921 agaaaattac agagaagaat gatataagta taatagccca aattctcttc ttatccatgc
     4981 ttgtaagagg agacttatct ttttcttcaa ctttttcagc aactacacct tctttaaaag
     5041 gtctctttcc tgcatttcca aggaatctcc atccatatat gaaccatatt gcacctatta
     5101 cacaaagtac agctgctata aagaaacatt gagaaaatcc aagtacacca ttttttgcaa
     5161 atgttttaag gtaaagaata ccaactgcta tagtaccaat aaaagaacct atattaacaa
     5221 aagaatattg tactgaaaat gctgtatcaa gttcttcttg actatcaaaa agctgtccat
     5281 ttaccgcaga tacgttacct ttaaagaatg cagtaccaat cgaaactaag attatcattg
     5341 catttaccat agaaactgta gttgcaatag aaccaagata gtaacctacc cccatgataa
     5401 gcatacctat aggtatagta tatctagctc ctatccatct atctgatata tatcccccaa
     5461 ttataggtgc aaggtatgtg aatgcaacaa ggttggcttg cattatagca gcttgtcctt
     5521 tatctattcc aagaccacca tgaactatag ctgttgtaag gaatatatat attaagtact
     5581 ttgaagcata atatgctgct ctttcaaatg agaaagttgt tgagcagata taaaatccca
     5641 taggaaattt acccttcttt tttactgttg agctcataat tttaaatcct ttcttaattg
     5701 tatttttatt ttttacgttt tgtccaaatt ctttctttta tgaaagatat aatattatta
     5761 ttttgtaaaa caatttgtaa ttattattaa gataattgcg catttatttg tagagtacat
     5821 attctcatgg aataaaatta atacacttct taatcataaa tttaaattat ataaattcac
     5881 tattttcaga tatagttata aattaatata ttctcatcta ataccatgaa aaaaccttcg
     5941 ccatatgcaa ttcttatttt ttacgtgata gtattttaca aactaaaatc ccccttttgt
     6001 agcaacttct gtaattacat tttagtgctt ttttaaagga gtctcgaata aagcaacata
     6061 tattttgttc aaataatgtt tttatttact ttttaaattt atatattact tcactcaacc
     6121 aattctcaac ttaaataaat aaactactat ctattcacag attagaccta tttcttacac
     6181 attagtttat aataaacact gtgaatctaa acataaaaac tgttatttta aagtaattac
     6241 ccatatattc gctttgatac tatttattga ggttatactc tttatttata atatattatt
     6301 tataaaatac ctcaaaatat attattaata aaatacctca aactgaaaac tttttcacga
     6361 atatttattt ttcacctccc cttttttatt tt
//
LOCUS       DAEPOG010000194         6380 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEPOG010000194 DAEPOG010000000
VERSION     DAEPOG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.194"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..457
                     /locus_tag="KR432_003241"
     CDS             <1..457
                     /locus_tag="KR432_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBH0641751.1"
                     /translation="RTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNK
                     YPNYGLALSLNYPYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMG
                     PRGATGSIGPMGATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            626..817
                     /locus_tag="KR432_003242"
     CDS             626..817
                     /locus_tag="KR432_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBH0641752.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(1052..2650)
                     /locus_tag="KR432_003243"
     CDS             complement(1052..2650)
                     /locus_tag="KR432_003243"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBH0641753.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            3528..6170
                     /gene="adhE"
                     /locus_tag="KR432_003244"
                     /gene_synonym="adhC"
     CDS             3528..6170
                     /gene="adhE"
                     /locus_tag="KR432_003244"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0641754.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         2379 a          856 c         1045 g         2100 t
ORIGIN      
        1 acgaactata acatggaatg atagagtgtc tatggaaaat atcaggggat atttaccaat
       61 tgggataagt gatacatcca actatatttg tttaaatatt acgggaacta taaaagcatg
      121 ggcaatgaat aaatatccta attatgggtt agctttatct ttaaattacc cttatcagat
      181 ttttgaattt acatctagta gggattgtaa caaaccgtat atacttgtaa catttgaaga
      241 tagaattata gataattgtt atcctaaatg tgagtgtctt ccaattagaa ttacaggtcc
      301 aatgggacca agaggagcga caggaagtat aggaccaatg ggagcaacag gtccaacagg
      361 agcaacaggc aattcctctc agccaattgc taacttcctc gtaaatgcac catctccaca
      421 aacactaaat aatggaaatg ctataacagg ttggtaaaca ataataggaa atagttcaag
      481 tataacagta gatgcaaatg gtacgtttac agtacaagaa aatggtgtgt attatatatc
      541 agtttcagta gcattacaac caggttcatc aagtataaat caatattctt ttgctatcct
      601 attcccaatt ttaggaggaa aagatttggc agggcttact actgagccag gaggcggagg
      661 agtactttct ggatattttg ctggtttttt atttgggggg actactttta caataaataa
      721 tttttcatct acaacagtag ggatacgaaa tgggcaatca gcaggaactg cggctacttt
      781 gacgatattt agaatagctg atactgttat gacttaaaac gtgtctaaaa taatcttaaa
      841 aactatttag gttttattta aatgacaaaa gtatttttat atattgagtt ttacctattt
      901 tagaatgaat aaaataacaa taataataaa atatattcat aaaaatttta aatttatgga
      961 tttttattta actttattat caatatatgt ataataaaaa actgtctcaa atatagattt
     1021 gagacagttt tcgttattta aaaattttat attatttaaa atttttgatt gcagtagtta
     1081 aattagggac taattgtttt tttcttgata caacacctgg tgcaaatgta ccttgaacac
     1141 cttcaacatc aaatgcgtta gcaattaagc tatcttctgc tttatagatt aaataagaac
     1201 cttctttgat tatatctgta accgcaagaa ttagtttgtc ataatcagtc gaatttatat
     1261 aagataaaaa ctcatctttt ttagcaaata tagagtctat gtctaaggta aatacttgtc
     1321 caataccaac tctatgtcca ctcatattaa attctttaaa atccatattt actatttctt
     1381 ctatagtata ttcatctaaa gaagtaccgc atttaaacat atccatagcg tatttttcca
     1441 tgtctacttt tgctatttta cttaattctt cacaagcttt cttatccata tcagttgttg
     1501 ttggagactt aaataataat gtatctgata atatagcaga taaaagaagc ccagctattt
     1561 cataaggtat ctcaacattg ttttctttgt acatttgata aattatagta ctattgcatc
     1621 caacaggcat aactctaaat gacataggaa catcagtaga aataccacca agtttatgat
     1681 ggtcaattat ttcaactatg tttgcttgtt caattccatc agcactttga gcatattcgt
     1741 tatggtcaac taaaacaaca ttctttttag atgggtttaa tagatgacct tttgaaacta
     1801 aacctaaaaa cttattatca tcatctaata ttggataaga tctgaagttt gtttctagca
     1861 taatctcttt tatttcatcc atataatcat cttctttgaa tgtaattaaa ttttctgtag
     1921 cttctataga gtctaagaaa ctacattgat ttattacatt agaagtttta taattgtcac
     1981 tttcaacaga aacaacagac actccattta tcttagcaag agctagcaaa tcttttgaaa
     2041 tctctgtatg attagtaagt attaaaagtt taacttttgt atcaatagca tattcaatac
     2101 tatcatatct atctccaaca atagctatgt ctccttcttt taaagtctct attagagtct
     2161 ccattccaaa tgataaagtg actactctac cttttatatc ttcaccacag tccacaagaa
     2221 tagtaccatt aaggttttta caaatgtttg atagtgatgt gtgtatagtt gtaaagtgtt
     2281 gatgaagtag atttttagct atttctttca tagtaatgat accagcaaaa gttccatcat
     2341 cataagttac aggtagtgtt ttaacatttt tttcatccat aaggttataa gcctctaata
     2401 tagatgaagt tggtgttaaa ggtactactt tatcataatt taaatctcta acttgtattc
     2461 taacattatc taaaatttca ggtgcatcta ccttaaagta atccaaggca tatttagctt
     2521 cttttctgat atctcctaaa gcataagctg ttgcttttat tcctagtttg ttctttaaat
     2581 gtgctaatga aattgcagaa catattgaat ctgtatctgg atttttatgt ccgaaaacta
     2641 ataaactcat tttaatactc ctttcaaaac ttgaatataa taataattga taaaaatatt
     2701 atatcaaaag gtagtgagta tttgttgtaa aaagaaaaaa atttagtgaa aaaatattat
     2761 ataaaattta tttcttacat aaatactaca aatttaatat aaaaattatt attatatata
     2821 tttttaccca accaaacata gaatcaaatt tacttttatt taaaataaat tgattaaatc
     2881 tacctgggaa aaaattaatt aatatgttaa taaattaaaa atttataaat attttatact
     2941 gtaaaaaatt ttgtatatat attatcatta aaaaattaac ggtcttaata taaaaattat
     3001 ttatctatat gaaaagcata attattacat attgacttaa aaataactta tagagattag
     3061 aataatttta gtttgtatat tttttaaaac catacagttg ttataaaaag aaaaattgca
     3121 cacaagtcaa aaataagtgt tattttataa aaatacgaca aaaattttat ttactactta
     3181 tacaaataac atgcaaataa catataaaat ttaataagta aaaaaaatga aagatataaa
     3241 ttatcatttt agtattttat ttagttgaag taaattattc aaatgaaata tatctcattt
     3301 agataaaaaa ttttagtaaa agtaaaaaaa ataatatatt tgttaaaaaa taaattgaca
     3361 aaatattcag aaaaaaactt cttgacctaa gagagtaaaa taaataaaat ctaaggtata
     3421 gagatgaaat ctataaaatt attttaagta taagttaaaa atatagatgg tgcatttaag
     3481 atttataaaa attagagatg cataattatt ttagggaggt ttttagtatg gagaaaaaag
     3541 aaaaggtagt agaaaaatca aatgttgaag tatgtagtcc tgaaattgtc aacagtgttg
     3601 aaacattaag aatgagatta gaagaaatta gattggcaca aaaagaattt gcaacattta
     3661 cgcaagaaca ggtagataag attttcttag ctgcttcaac tgcagctaat caacaaagaa
     3721 tacctctagc aaaaatggca gtggaagaga ctggtatggg tattgttgaa gataaggtta
     3781 taaaaaacca ttttgcctca gaatatatct ataatgctta taaagatact aaaacttgtg
     3841 gtgtcataga aaaagatgaa gcatttggtt ttacaagaat tgctgagcca gtaggtgttt
     3901 tatcagcagt tataccaaca actaatccta catcaactgc aatattcaaa tccctaatag
     3961 cactaaaaac tagaaatggt ataattttct ctcctcatcc aagagctaaa aactgtacta
     4021 tagaagcagc tagagttgta catgatgcgg cagttaaggc aggagcacca aaaggactta
     4081 taggttgggt agatgttcct tcgattgaat taactaatgt tgtcatggca gaggcagacc
     4141 ttatattagc tactggtgga cctggaatgg ttaaatctgc atactcttca ggtaaaccag
     4201 ctgttggagt tgggcctgga aatgtacctg caataataga tgagagtgca gatataaaaa
     4261 tggctgtaag ttcaatctta gtatcaaaat cattcgataa tggtatgata tgtgcttccg
     4321 agcaagctgt aatagtacca gaaaaaatat atgaagaagt taaaaaagaa tttaaatacc
     4381 gtggagcaca tttcctaaat aaagaagaaa ctgaaaaagt aggaaaagta gtaataattg
     4441 atggttcatt aaatgccaga atagttggac aaccagcaca tgtaatagcc aaaatggcgg
     4501 atgtagaagt tccaaaaaca gctagaataa taattggtga agtagaatct gttgaattaa
     4561 atgaaccttt tgcacatgaa aaactttctc ctgtacttgc aatgtacaaa tctaaaagtt
     4621 ttgaagatgc agttgctaaa gctgaaaaat tagttgcaga tggtggatat ggccatacat
     4681 cttcgttata tgcagactct ataaatcatc cagatagagt agagaagttt gtaaatgcta
     4741 tgaaaacttg tagagtatta gttaatactc catcgtcaca aggtggtata ggtgacttat
     4801 ataactttaa attagcacca tctcttactc ttggttgtgg ttcttggggt ggaaactcag
     4861 tatctgaaaa cgtgggagtg aaacatttac ttaatatcaa gacagtagct gaaaggagag
     4921 agaatatgct ttggtttaga gcaccagaaa aggtttattt caaaaaaggt tgtttaggtg
     4981 tagcagcaag agaatttaaa gatgttatgg ataaaaagaa agcctttata gtaacagatt
     5041 ctttccttta caataatgga tacactaaga agctaactga tttgttggat gagatgggaa
     5101 ttaagcatac tacattcttt gatgttgctc cagacccaac attagcttgt gcaagagaag
     5161 gagctaaggc tatggctgat ttccaaccag atttaataat agctgtaggt ggaggtagcg
     5221 caatggatgc tggtaagata atgtgggtaa tgtatgagca tccagaggta gacttccaag
     5281 atttggctat gagatttatg gatataagaa aaagagtata tgtatttcct aagatgggtg
     5341 aaaaagcgta ttttgctgct atcccaactt cagcaggaac tggttcagaa gtaactccat
     5401 ttgcagttat aactgaccaa gattcaggtg taaagtaccc attagcagat tatgaattaa
     5461 tgccaaacat ggctataatt gatgctgata tgatgatgga gatgccacca agattaacag
     5521 cagcatcagg tgttgatgct ttaacacatg cattagaagc atatgtatct atgttaagaa
     5581 ctgaaccagc tgacggtcta gcactacaag caggtaaaat aatatttgaa tatctacctc
     5641 gtgcatacaa aaatggaaag aatgataaag aagcaagaga aaaaatggca atggcatcta
     5701 ctatggcagg aatgtctttt gcaaatgcct tcttaggaat atgtcactct ttagcacata
     5761 aattaggtgc attccaccat gttcaacacg gtgttgcaaa tgcattatta ataaatgaag
     5821 taattaagtt caattgtgca gaagctccaa ataagatggg tgcattctca caatacagat
     5881 atcctgattg tatacaaaga tatgctgaat ttgcttcttt tgcaggaatc aagggaaata
     5941 cagaccaaga aaaagtagat aaccttataa aagcaataga tgaattaaag gcaagagtag
     6001 gacttcctaa aactataaaa gaggctggag tagaagaatc taaattctta gaaacattgg
     6061 atgttatggt tgaacaagct tttgatgacc aatgtacagg tgctaatcct agatacccat
     6121 tgatgagtga attaaaagag atatatctaa aagtttatta tggtaaataa tattcattgt
     6181 acaacatagg ttctattggg tttgtatata aattattaga tttgaatata aatagagttt
     6241 tattagagaa acgtagaagt aactataaat aatagattat aacttaaatt ctgaatggaa
     6301 atgataaaaa gtcagtaatt taatggtaat ttcgaatatg tgtatttaat caattaactt
     6361 taaaattagt cggaaaattt
//
LOCUS       DAEPOG010000195         6356 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEPOG010000195 DAEPOG010000000
VERSION     DAEPOG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.195"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(216..1403)
                     /locus_tag="KR432_003245"
     CDS             complement(216..1403)
                     /locus_tag="KR432_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0641755.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            1676..2500
                     /locus_tag="KR432_003246"
     CDS             1676..2500
                     /locus_tag="KR432_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0641756.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            2629..3894
                     /gene="gluD"
                     /locus_tag="KR432_003247"
     CDS             2629..3894
                     /gene="gluD"
                     /locus_tag="KR432_003247"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBH0641757.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            4087..4743
                     /locus_tag="KR432_003248"
     CDS             4087..4743
                     /locus_tag="KR432_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0641758.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            5074..6207
                     /locus_tag="KR432_003249"
     CDS             5074..6207
                     /locus_tag="KR432_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH0641759.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
BASE COUNT         2462 a          684 c         1017 g         2193 t
ORIGIN      
        1 aaaataggat atttgatgta tgttatgcag acttttttaa tataaaagaa aatggagaat
       61 tttgttatag tgtatagtat atacgtggag taaggtttat aaatacagta aatcaataaa
      121 tagagttatt ctaaaataag aaaaatgaca aatcttattt agaaatagct ctattttttg
      181 taattaaatt taaattatat tcaatttgta aaatattatt ttgaacaatt atacatatac
      241 tctctagtta gaggtaaatc attttttata cctttagtga aaagtatttg atgtatatct
      301 ataataccag tgttgaaagc agcagcacaa gaacaaaggt ataaatccca cattcttata
      361 aacttatcat caaacatttc tttaatcttt ggaagattct cagagaaact tttttgccag
      421 tatagtaagg tttttacata atgtaatctt aaactttcta cgtctatagt atgatatttg
      481 taatcactac aaatatgtat catttctctt aggctaggaa taactcctcc tgggaaaata
      541 tattttttta tccaagggtc tccataagat tcctgcaatc cacttatata gtgaagtaaa
      601 aacaatccac catcttttaa aacagcattt acgtttttaa tgaaaagttc atagttttct
      661 ctaccaacat gttcaagcat tccaacgctt acaactctat caaacataag gtctgatttt
      721 tttaattctc tatagtccat aagtcttacc tcaagatagt cttgaaggtt ctcttcttta
      781 attcttgcgt taaattcctt aaattgctct tcgctaagtg taattccaac gccgtgaatt
      841 ttatattttt ttgcagcttc tattagtaaa aatccccaac cacaaccaat atcgagaagg
      901 ctcatacctt cctctaaatt tagtttagtt aatatgtggt gaactttatt gacttgagca
      961 tcataaagag tatcattatc atttctaaag tatgcacaag aataactaag tgtcttatct
     1021 aaccataagc tataaaaatc gttgccaatg tcatagtggg catgtatttc tttcttctga
     1081 tgtctcattg aatttgatga gtgcaataga tgttttacag ctgaattatc aatattgaat
     1141 ttgtctattt gttttaaaag tagatttaaa gttaagaata aatctccctc aacttcaatg
     1201 tctctattta tgtaggcttc tccaagtgct aaagatgtac tttttaatag ttcagacttg
     1261 ctaatatcat tatttactat aactttgaac tgaggggagc cctctccaat tagaaattct
     1321 tcctcatctt taagcctaac tagaaatggt gtttcaccaa atttctctaa atagtctttt
     1381 aaaaaatttt ttgtaatatt catattacca gctccttcta atgtaaatcc agtaagtttc
     1441 cctgcttaaa atatattata gtcctttttt taaaaaagta aaattttttc aataaatttt
     1501 ttatttaaag gaaatttatt ttttataaag aaatttcatg ttgtagtata ttttttgctt
     1561 ataaattttg gtactggata taatattaaa ctgttaatga tataataaat ttaatctata
     1621 aagtaaataa aattttgtaa taaatagtta aagctatttt ataaggagat atatgatgga
     1681 agtatatgct ttggttgggt caagtggcac agggaagagc tttaaggctt tagagttcgc
     1741 atatgaaaat gatatagaat atataatcga tgatgggatt ttaatataca aaaacaaggt
     1801 tttagctggg atatcggcaa aacaagcaaa tactactata gaagcagtta aaagggctat
     1861 tttttataat ctggagcata gacaagaagt tagagaagca attaaaaaag aaaatataag
     1921 aaaaattctt attattggga catcaaaaaa aatggttaat caaattgtag agagattgtc
     1981 aattggaagg gtctgtaagt ttataaatat acaagatata agtactaaat atgaaataga
     2041 aatagcaaaa caagctagaa aagaaggaaa tcatgtaatt cctgttccag ccgttgagat
     2101 aaaaagtatg gcaagtggtc tcagtataaa ttctttgaag agacttttta gaaaaggaaa
     2161 taataggaat atggtagttt tagaaaaaac aattataaga ccaactttta gttatatagg
     2221 aaagttttat attagtgctg atgtaattaa acagattatt gaatatgaag tatacaactt
     2281 tggaagcatt gatagaataa ataaaatcaa tatagagaat tgcaataatt ttatgaatat
     2341 ttttgtaagt ataaatatca atgatttaga aactataaaa aaaattgaag acatacaaca
     2401 gacaataaaa aagtctattg agaaaatgac tttagttaat gtacaaaaaa tagatattaa
     2461 cattcataaa attaaaaaaa taaacttcct ccaatgttaa aaaataaact gagaaaatga
     2521 tatactaatt tgcaataaag tgtgctataa ttaaactgta aatttaggaa atggtttttt
     2581 ctaaatggag tatacataat tataacgtat ttataagggg gctttcaaat gtcaggaaaa
     2641 gatgtaaatg tcttcgagat ggcgcaatct caagtaaaaa atgcatgtga taaattaggt
     2701 atggaaccag cagtttatga attattaaaa gaacctatga gagttataga ggtttcaatt
     2761 ccagtaaaaa tggatgatgg ttctataaaa acttttaaag gatttagatc acaacataat
     2821 gatgcagtag ggccaacaaa aggtggaata agattccatc aaaatgtttc aagagacgaa
     2881 gtaaaagctt tatctatatg gatgactttc aagtgttctg taacaggtat accatatggt
     2941 ggaggtaaag gtggaataat agtagatcca tcaactttat ctcaaggtga attagaaaga
     3001 ttaagtagag gatacataga tggaatatat aaattaatag gtgaaaaagt tgacgttcct
     3061 gcaccagacg taaatactaa tggtcaaata atgtcttgga tggttgatga gtacaataaa
     3121 ttaactggac aaagttctat aggtgttata actggtaagc cagttgaatt tggtggttct
     3181 ttaggaagaa cagctgctac tggatttggt gttgctgtta ctgcaagaga agctgctgct
     3241 aaattaggaa tagacatgaa aaaagctaaa atagctgttc aaggtatagg aaacgttggt
     3301 tcttatacag ttcttaactg tgaaaaactt ggtggtactg ttgtagctat ggctgaatgg
     3361 tgtaaatcag aaggttctta tgctatatac aatgaaaatg gtttagatgg tcaagctatg
     3421 ttagattata tgaaagaaca tggtaactta ttaaacttcc caggagctaa gagaatatct
     3481 ttagaagagt tctgggcttc agatgttgat atagtaatac cagctgcatt agaaaactct
     3541 ataactaaag aagttgctga atctataaaa gctaaattag tttgtgaggc tgctaatgga
     3601 ccaactactc cagaggctga cgaagtattt gctgaaagag gaatagttct tactccagat
     3661 atattaacta atgctggtgg agttacagtt tcttacttcg agtgggtaca aaacttatat
     3721 ggatactact ggtcagaaga agaagtagag caaaaagaag aaatagctat ggttaaagca
     3781 tttgaatcta tttggaaaat caaagaagag tacaatgtta caatgagaga agctgcttac
     3841 atgcattcaa ttaagaaagt tgctgaagct atgaaattaa gaggatggta ctaagattta
     3901 ctcttaataa ataataggat ataattattt aagatataaa ggggctgtac atagtacagc
     3961 ccttaaatta tgtggcttac aatgaagtaa gctatttttg ttttaactta taaataactt
     4021 gataaattat gtaatttaga atgcataaat aagatgggtg aaaattaatg gttgatttaa
     4081 ttttgaatgt attttttacc accacatgat tacaaattag aggagttaga ttggattttt
     4141 ggacatgaac ttatgtattt taaaagcaaa gaccttcatt taaagtatct agtattattt
     4201 ttaaaaatag tatattggtt taatccattt atatatataa tggaaaaata tatggatttg
     4261 gattgtgaac tttattgtga tgagagagtt ttaaaaaatt gtactgtaca acaaaagcaa
     4321 gaatatgctc taactattgt aaattcaatg aaaagaggta taaatttttc aaataaattt
     4381 gtaactggat taaataaaca atctgatatt agaaaaagag taatgaacat gttcagtgaa
     4441 aaacataaaa atggtatctt aatggctttg attataagct tgttttcttc tattacattt
     4501 tttaaattcg attcaactag tcttattgaa gagaatgatt tcttagagag gtcaagatat
     4561 gcgtttgaag gaaggcaaga agacatgttt gtaacaatag attctacata tgcagatgca
     4621 cctataaaac ttagaaaaga gcatgaagaa aactgtagag tcaatggaat agtaccaaaa
     4681 gattcagatt caatagaaat tattcctaga ttttataaag attgtatgga tttaaaaaaa
     4741 taagttagac ttaatattga tgttgatatt atgaattttt tatatttaag caattgaaaa
     4801 ttttattata gattcaattt tatatgtgtt taagtatgaa atttatagat agttgaaata
     4861 attaaatatg attgatgcat aggaatcatt actaagctat aattaattat aggggagttt
     4921 aaataaatta tgagttacac aattataaaa tgataaaaaa tagttatctg ttacaaaaaa
     4981 tgtatctttt taattaataa agataatatg aaaacttaaa aggcaaataa acttaaacat
     5041 aaaatactct taaataagat aggagaaaaa tgtatgtcta actttattct aaatgggatt
     5101 tttagaacag ttatagtaag tagtctaagc attcttctga tattgatttt caagaaaaat
     5161 gtatttaaaa gatttagcaa aaaatttaat tattatatat ggatgatagt tgttattaaa
     5221 ttatttttac catttacata ttatactttt acaataaata tcttgagaag taaaaaacac
     5281 attaatatta ataacataaa tttagaggga tttaataatg tttcaactat aagtaataat
     5341 attatattgt acacttggat tataactgta gtagtgtatt taatttacac tatatttaaa
     5401 tatataaaac ttaaaaattt aataaatgac ttatcttatg atgttgatga tagagagatt
     5461 gtaaatcttt ataaaaatat attagaagaa tttaatataa caaaagatat aaaactcaaa
     5521 tactcttatg aagttgaaac accagcattt tttaattcat gtgtactttt gccaccacat
     5581 gagtataaat tgaaagagtt agactggatt tttagacatg agcttatgca ttttaagagt
     5641 agggaccttt atttaaaata tgtaatcctg tttttaaaaa cagtatattg gtttaatcca
     5701 tttatatata taatggataa acatatagat ttagattgtg aactttattg tgatgaaaga
     5761 gttttaaaaa atcgaaattc agaggaaaaa aaggattatg ctttaacaat tctaaatgca
     5821 atgagaaaag gttcaaatac ttcaaataaa tttatagctg gactccataa gcaatctgat
     5881 attaaaaaga gagttatgaa tatgtttaat gaaaagtaca agaatggtat attaatggca
     5941 ttaactttat gtttattatc ttctattact tttttgaagg ttaattcaac gagtattgtt
     6001 aatcttaatg tattacctaa aggatttgaa gacttacgaa ctgttagcag tatacgtttt
     6061 tcagttatag gatttacata tgcagatgct cctaaaaata tgagggaaga atataaaata
     6121 aattgtgaat cattaggttt gatacctaaa gactctgata aaatagaagt tggtgtagaa
     6181 tattatgaca gtctattgaa tgattaaata aatattattt taacagatat taattataaa
     6241 gttttgaata gtaatttgtt gagaaataaa tatatcctta taaaaataga tacttatata
     6301 ccaagaataa aaaatagtta agatagtaca caaggatata cttataaaga acttaa
//
LOCUS       DAEPOG010000196         6314 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEPOG010000196 DAEPOG010000000
VERSION     DAEPOG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.196"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..597
                     /locus_tag="KR432_003250"
     CDS             <1..597
                     /locus_tag="KR432_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0641760.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="KR432_003251"
     CDS             728..1750
                     /locus_tag="KR432_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0641761.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="KR432_003252"
     CDS             complement(1914..2471)
                     /locus_tag="KR432_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0641762.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="KR432_003253"
     CDS             complement(2477..3553)
                     /locus_tag="KR432_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH0641763.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="KR432_003254"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="KR432_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH0641764.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="KR432_003255"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="KR432_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0641765.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..>6314
                     /locus_tag="KR432_003256"
     CDS             5391..>6314
                     /locus_tag="KR432_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641766.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAK"
BASE COUNT         2169 a         1041 c          838 g         2266 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaagtccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa taataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggataggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg ttcctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaata tgattgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttacaggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatgttt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt ttccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattactt cttaaatgat
     3061 ggtccagtgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaaggatat ctcccttcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc ccccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ttgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttcgccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga cagcatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttattta
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggtga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcaacttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctgtttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaatatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagctgatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaa
//
LOCUS       DAEPOG010000197         6285 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEPOG010000197 DAEPOG010000000
VERSION     DAEPOG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.197"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            226..1593
                     /locus_tag="KR432_003257"
     CDS             226..1593
                     /locus_tag="KR432_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH0641767.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1618..2226
                     /locus_tag="KR432_003258"
     CDS             1618..2226
                     /locus_tag="KR432_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBH0641768.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            2274..2645
                     /locus_tag="KR432_003259"
     CDS             2274..2645
                     /locus_tag="KR432_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH0641769.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2824..3162
                     /locus_tag="KR432_003260"
     CDS             2824..3162
                     /locus_tag="KR432_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH0641770.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3629..4132
                     /locus_tag="KR432_003261"
     CDS             3629..4132
                     /locus_tag="KR432_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBH0641771.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4179..5138
                     /gene="moaA"
                     /locus_tag="KR432_003262"
     CDS             4179..5138
                     /gene="moaA"
                     /locus_tag="KR432_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH0641772.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5142..5612
                     /gene="moaC"
                     /locus_tag="KR432_003263"
     CDS             5142..5612
                     /gene="moaC"
                     /locus_tag="KR432_003263"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH0641773.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5648..6079
                     /locus_tag="KR432_003264"
     CDS             5648..6079
                     /locus_tag="KR432_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBH0641774.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2656 a          498 c         1135 g         1996 t
ORIGIN      
        1 aaaattactc atttagttta ataactctca tttcatatgt aaagttggta aaaaaattaa
       61 aattaaaagt aaaaaactaa ataaattaat gttaatttga aatatatatg aaggtttttg
      121 gagaatactt acttatgata ggaaaaaatg ttaaaataat agtgtaatca aatataaagg
      181 actgaaataa ttaacagtta ttaaatatta ggagtgtaaa aatttatggg attaaaagaa
      241 tttgatttta ttgaagagtc tattgatatg ttgaggacaa tgtcaccaac attagaaact
      301 atatcagatg aaatagaaga gtattttgaa aatatcttgg atgaaaagaa tcaagaatat
      361 ataaatgtaa cctctagaat aaaatcagaa tcaagtttaa gagaaaagat aattagaaat
      421 agatatttaa aaaaatatgg agaagcaagt aatcttattc ataatgtttc tgacttaata
      481 ggcctaagaa ttgagtgtag atttattgag gatgaaaaca aaatatatag acttttgaga
      541 agatatttta ataaaacaga tgacaaaata aactattata ataaagagaa taagaatatt
      601 aaattaaaac tatctgagag gcaacccaat aagcaaaaaa atggatttga aatatataaa
      661 atagatggag tttttgcgta tttagataga gaagtaaaat ttgaacttca aattaaatct
      721 cttgtaaatg tgttttggag tgaaatagaa cataaaataa tttataaaaa taatacatat
      781 ttacttgctg ataaatttat aaaggatatg atggactcta taaagaataa tcttactatg
      841 atagataatc agcttttaag tatttataaa aactttcatt cagggaagtc ttttaatatg
      901 aaagtaagta aaaaagaaat tgaaaaatta tttgcgaaac tagtctatga cgctttttct
      961 gaaaagatga ataaaagtat aggttttgtt gtagatttta aaaaaccatg tgaaactata
     1021 ttgaactatt cttttaataa acaagatata agtgatgaag ttttaggtaa ttttatgctt
     1081 gatgagtttg caagattaaa tgaaattgta aacaaggata tagattttaa tgagcaaata
     1141 gaatttgaaa gagaaccaac atttgatgat aagttttgta aagacctagg aaatcatttt
     1201 agaagtagat taaacacaga atttccatgg aacctatttt ttagaatatt atttgaaata
     1261 gagccatata acaataccaa agactttgaa aactttgtag agtttataaa agaaaatgtg
     1321 ctactagaag aaaataggga gcaattatta tgccaatttg aagaagatag caagcttata
     1381 attgaagata tatatgattg tatatttaat agtataagtg agattgatag tgttgaaatt
     1441 ttatataact ataatcttga aaaaataaac tttacatcaa gtgaaatcat aaattgtata
     1501 tgtagagaat atgagtgtta tggtgaatac ttagaagaaa aagaaaagct tatgaataca
     1561 tttaaagaaa agattattca aatctttgag taataggtca atgctgaagt ggcgctaatg
     1621 cgccattttt caaatatata tgaattaagt aaaattattt tggaggaggc gatttgtata
     1681 gagaaaacag gtgtaatttt ggcaggggga aaaaattcta gaatgggtag agataaagcc
     1741 tttttagagt tacatgataa gctttttatt gaaattgcaa tagaagcatt taaaaacttt
     1801 gatgagttga taataatatc taataatgaa gaattatatt ctaaatacga tattaaagtt
     1861 tataatgata tagtaaaaga tgttggtcca attggaggaa tatatactgc tcttgaatat
     1921 gcaaagtatg atatagttac aattgcatgt gatatgccat atctaaataa tcaaatagtc
     1981 gaaagaatag ctaataaaat gaatgataaa agtgtgattt cagtgacaaa tggaaagtta
     2041 caaccattgt gttctggata taaaaagagt ataataaata aagtatcttt atgtataagg
     2101 gaaaatgatt taaagctcag aagctttata gataaaatag ataaaagtta tatatacttt
     2161 gatgaagaag atttattttt aaatgtaaac actgttgatg agtatgaaaa attaactaaa
     2221 gtttaattag ctatggttgt aaaatagagt aggatttata tggaggagag tttatgaatt
     2281 tacttaaatg cgattcttgg gcagtaatat taaataatag cgataaagaa agtaaagcat
     2341 ataaaatatt agatgaactt aaaaggaata tgtacaaagt tgttgctata gatgaagaga
     2401 aaaaacctat tgaaggaata gatgtgtatg aatgtttaaa agatgttcca cacaatattg
     2461 atgttgtagc tattattgat aaacaatcta agatggatgt gattttagaa gaagtagagc
     2521 ttttggatat acaaaatatg tggtttgaaa aaggaagctt tagtgaaatg atgattaaaa
     2581 agactaaaga tttaaaatta aatatagaat ataatttaag tttatatgat gagttgacta
     2641 gataaaatta tatttcttaa taataaaaat ataattagtt aataaaacat attattttat
     2701 agtatagatt ggttaataaa agtacagatt ggttaatgaa acattaaatt acaatatttg
     2761 ttaaaagaat tgtgagttac atgatataat gtattaaata ggatactaag gagttgatat
     2821 attatgttaa tagtaactac agaaaaagta gaaggtaaaa agatatcaaa ggttttagga
     2881 ttagtgagag gaagtacaat aagagcaaaa catgttggaa aagatatagg agcaagtttt
     2941 aagaatcttg ttggaggaga acttactgga tataatgaaa tgctcactga agcaagacaa
     3001 attgccatag gtagaatggt tgaagatgca gaagctaaag gtgcaaacgc agtaatagca
     3061 tttagactgt cttcagcttc agttatgcaa ggggcagcag aaatgcttgc ttatggaaca
     3121 gcagttgttt tagaagatga taatagtatt cttgaaaaat aaaatataaa tagttaaaaa
     3181 gaggcatctt ttaaaaattt tatggatgcc tttttttatt tgaaaaaact aatttgagaa
     3241 gaaattttaa ctattatgac aataaaatta gggtgatatt atataaataa gtaaaattat
     3301 attatataat gtatggtata gactttaaaa attttatttg tagagaaaaa tatggacaat
     3361 gttataataa ttatagaaat taaaagtact tacattcatt atgtatgtga catgtataat
     3421 tagttttata tttaatacag ttttgaagta atatcttcat tttgagaacg ttggaataat
     3481 aaaaaatgta tgtattatag gtgatataaa taaaaaataa atataatatg ttatattgct
     3541 gatagaaaag agaataaaag aaaataggtt ttattaaaag agatttttat ttataggact
     3601 taatcaatgt atagaggggt gagaacatat gtttaatgtt gcaattataa cacttagtga
     3661 taaaggatat gaaggaaaaa gagaagatat aacaggaaaa aagttaactg aatttgttga
     3721 aaatacagga gcttataaag ttactgagta tgtacttata aaagatgata aggaaatgct
     3781 aaaggaaaat attataagac tttgtaatag taataaaata gatttaattc ttacaaatgg
     3841 aggtactggt ttttcaaaaa gagatataac cccagaagca actaaggaag tgttggaaaa
     3901 agaaatacca ggtctgagcg aatatatgag aatgaaatcc acagaaatta ctaaaaaggc
     3961 catactaagt aggggtgtga gtggaataag aaataattct ataataataa atttacctgg
     4021 aagtcctaaa ggtgcagttg aaaatctaag ttttataatt gatgttttag accatggcat
     4081 agaagtttta agaggagaag ctacagaatg tgccactaaa aaaagtgaat agtatataat
     4141 ctagatagat aaaaaataga caaagaggta gataaagtat gattgataag tatggaagaa
     4201 aaatagacta ccttcgtatt tcacttacag ataagtgtaa tttaaggtgt gtctattgta
     4261 tgccaccaga tgtaaaattt gataaaaatt atataaatga aaatctaagc tttgaagact
     4321 acaagtttat tataaaggct atggctgaac aaggtataac aaaaattaga tttactggag
     4381 gagaacctct tttatatcct aaattaaatg agcttataaa atttacaaaa gaagaatgtg
     4441 gaataaataa cataggaatg actactaatg ctattgggct ttctgataga atttttgagc
     4501 ttgaaaaatg tggcttaaat aaagttaata taagtttaga ttctttgaag gaatataagt
     4561 ataaatccgt aacaagagga ggaaacttaa aagatgtctt aaaatcgatt gaatcttgtt
     4621 taaacttagg tataaaagta aagttaaatt gcgttgctat aagtgggttt aatgatgatg
     4681 agcttaaaga ttttatgttt ttaacagtta aatcaccaat tgatgtaaga tttatagagc
     4741 ttatgccatt aggtgaggct aatagagttt ataagaaggg ttattttaat gtaaaagaaa
     4801 tgattgaaaa aatatatggt ttatataaag tcaaaaatga tgaaaaaagt acagcagaat
     4861 attatatgtt taaaggagct aaaggaagaa taggaattat aactccatta agctgttcat
     4921 tttgtaacac ttgtaatcgt attagactta cttctagtgg tgcaataaaa ttatgtctac
     4981 attcaaaaga agaaatagat ataaaaccat ttttacataa accattgtta tttagagaat
     5041 ctatgaaaga tataataaaa caaaaaccag aaaaacatca tttaatagaa aataaatgta
     5101 gtgatacaaa taggcgtatg tatgaaattg gaggataagt aatgttaaca catataaatg
     5161 aaaaagggta tgccaagatg gtggacataa gtgaaaaaga tgatactaaa agaacggccg
     5221 tagctacagc tacaattact cttagtaagg aagcattaga aaaagtaaaa aatggtcaga
     5281 taaaaaaagg agacgtattg tctgtagctc aagttgcagg tataatggga gctaaaaata
     5341 catcttcaaa cataccaatg tgccatcaga ttaaattagt tggatgtgat ttagaatttg
     5401 atgtagatga ttatatgtct gctattaaga tatacgcaac ctgtaaatct cttgggaaaa
     5461 ctggagtaga aatggaagct ttaaatgcat gttctttagc agcacttaca atttatgata
     5521 tgtgtaaagc agttgataaa aaaatggtta taagtaatat ccatcttgtg gagaagactg
     5581 gtggaaaatc aggagatttt aagttcgagt aaattaataa tatattgggt taaaatttgg
     5641 aggaaatatg gcgaaggtag tgtcaataaa tataagtgag aaaaagggtg taattaaagt
     5701 tcctgtacca tgtgcagagc taaaaataaa tcatgggata gttggagatg cacatgcagg
     5761 aaactggcat cgtcagataa gcttacttgg aaaagaaagc atagataaaa tgaaaatgaa
     5821 aggctttgac caattaaaat atggagattt tgctgaaaat gtaactacag aagggataga
     5881 agttttttcc ctgcctgttg gtacaaagtt aagaataggg caatgtgaag ttgaagtaac
     5941 tcaaataggg aaaaaatgtc acaatggatg tgaaattaag aaattgacag gtgactgtgt
     6001 gatgccacga gaaggaattt ttgtaaaggt tctaaaagaa ggaattataa atgtgaatga
     6061 ttcaatagat gtaatctaat gaagtcacta ttaaaatata attataagta aatactatta
     6121 agatttaaat atatatttta agagagaatt aatttaaata aaatatttta gttataaatt
     6181 ggaatattgg ttatataaag ctatgaatgt aaaatcatag ctttttgttt ttgaatttta
     6241 aaatataata ttttatttat atatattaaa ttatatttta tataa
//
LOCUS       DAEPOG010000198         6244 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEPOG010000198 DAEPOG010000000
VERSION     DAEPOG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.198"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            355..921
                     /locus_tag="KR432_003265"
     CDS             355..921
                     /locus_tag="KR432_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0641775.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            961..1542
                     /locus_tag="KR432_003266"
     CDS             961..1542
                     /locus_tag="KR432_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0641776.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1599..2516)
                     /locus_tag="KR432_003267"
     CDS             complement(1599..2516)
                     /locus_tag="KR432_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0641777.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2646..4277
                     /locus_tag="KR432_003268"
     CDS             2646..4277
                     /locus_tag="KR432_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0641778.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCRVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4280..5227
                     /locus_tag="KR432_003269"
     CDS             4280..5227
                     /locus_tag="KR432_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH0641779.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5512..5775)
                     /locus_tag="KR432_003270"
     CDS             complement(5512..5775)
                     /locus_tag="KR432_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBH0641780.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5874..6119)
                     /locus_tag="KR432_003271"
     CDS             complement(5874..6119)
                     /locus_tag="KR432_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641781.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2258 a          843 c         1002 g         2141 t
ORIGIN      
        1 ataattgtta tttttaagat aaaaattaaa tgtatcaatt attgttttat acaagtattt
       61 ccaaatacgt tctcatttag atagtgtgct tagaaatttt agaattattg agtctaactt
      121 tattacttac tttactttat aacaatcaaa tacaaattta aatattataa aactacaaaa
      181 taatttaaat catataatgt caatatgttt aaattataaa atattcatgt agttatataa
      241 taaataattt ataaatatag ccaacttagc ctaaatacgt atataatata gctaatatgg
      301 ttcttatact tgataccatc aataaaacta aaaatataaa aaggagaact taaaatgagt
      361 gttagtttac aaaaaggaca aagagttagc ttagtaaaag ataataatcc tgtaaaaaat
      421 ctagtagtag gcttaggatg ggatatgaat aaattgggta aaaaaaatta tgacctagat
      481 gcatttgcaa tagcattgac taaccaagat aaaatgaatg ttagaggtga cttggtttat
      541 tttggaaatc ttaaacaccc ttctaaagct attatacaca ctggagataa cttaactgga
      601 aaaggtgaag gtgatgatga acaaataatt gttaatttag aagacatacc tgaatatgta
      661 cataaaatag tttttggagt ttctatatac aaagctaaaa aaagagacca agattttggt
      721 caaatgaaca actcttttat aagattaatt gattcaaatt ccaagcaaga attatttaaa
      781 tataatcttc aagaagactt ttctggtaaa gtaacagttt tggcaggaga aatttatcgt
      841 agaaatgaag aatggaaatt taatgcttta ggaattggtc aaaatgaaga gcttcgtgaa
      901 ttaattaata catataagta ataattacaa taaaaaattt ataatgagga gttggtttaa
      961 atgggtataa ctttagcaaa aggacaaaaa gtaagtttaa caaaaagtaa ccctggtcta
     1021 aaaaaagtaa tagtaggact aggatgggat attaataaat atgatggtgg atttgatttc
     1081 gatttagatg cctctgcatt tttaactgga actgatggaa aagtaacgaa tgatggagac
     1141 tttatattct ataataattt aaaacacgct tctggagctg ttgaatatat gggtgataat
     1201 agaacaggtg ttggtgatgg tgatgatgaa caaataaatg tagacttatc taaaatacct
     1261 caaaatattg ctaaaatatc atttagtgtt actataaatg aagcaattac aagaagacaa
     1321 aatttcggac aagttgaaaa ttcatatata agaatatata atgaagaaac taacgaggaa
     1381 cttattaaat atgaattggg tgaagatttt agcatagaaa cagcaatagt cgtggcagaa
     1441 ctatatagac ataatggaga gtggaaattt aatgctttag gttctggatt tgaggatgga
     1501 cttgcaggat tgtgtaaaaa ttttggcgtt aatataggct aatatatcta aataaaaata
     1561 agagtatatt gatatactct tatttttatt tagatatatt ataaactgtt tacacttcta
     1621 ctcttctttc tcgcttgttt tttcccatat tctactgaaa tatcaaaaaa tgctgaaata
     1681 ttttctgtaa aaaacttgct tctatgatat gcaatatcga atgttctatg taagttaata
     1741 ccttcaatat cacaagccca caaaagatgc ttttttaaat aatcctcaat aagtaattcg
     1801 gatataactg caatgccaaa attatctaca actgctctta atatagcttc agagttataa
     1861 catgaccatc taatattcat tgggattttt aattctttta attgcttttc taattgtgct
     1921 cttgtaccac ttcctaattc acgcataatt aatggttgat ttgaaagttc tgaaactttt
     1981 atagagtctc ttttgtagaa ctcatgcttg tgtgaacata taagtacaag attatcatta
     2041 ataatgcttt ttgtaacaat atctgggtgc ttaatttttc cttctacaat cgcaatatct
     2101 aattcactat taagtaattt ttttgcaatc agtcttgtat cttctacaaa tacttcaggt
     2161 tctataagag gatttacctt gtataattct aacattatcg ggcttattac acatgtacca
     2221 acagttactg ttgccccaac tcttatacgg atattttttg cacaatgctt catacaatca
     2281 ttcatttctt catttacagc taaaagatgc ctcccatatc caagaagttt tttccctgcc
     2341 tcagttaaat ataatttttt tgaaattcta tcaaataact taacaccata ctctttttct
     2401 atttctgaaa tagcttggct aactgatgac tgggtaatat acatatttct tgctacttca
     2461 ctcattttac cacattcagc aaccttaaca aagatttcaa gactacgaat tgtcatatta
     2521 tcacaacctt agcaaacatt agtaaatgct tatgatttta ttaatatttt agtattttac
     2581 aaatatattg tcaagaagta taattttatt atagagttat aaagtagatt ggagtgtgat
     2641 atattgtgaa agtattaatt ataggtggag ttgctgctgg aacaaaaact gctgcaaaaa
     2701 taaaacgtga aatgggtgat aattgctctg ttacaatact aaataaaggt gatgatattt
     2761 cttatgcagg ttgtggtctt ccatattacg ttggtaagat aattgaagat aaatcatctt
     2821 taatcgtaaa tacacctgaa agtttttcta aactaacagg agcagaagtt cgttgtagag
     2881 ttgaagttac gtctattgat agaaatgcaa aacaagtaac tttgaaaacg ctttcaacag
     2941 gagaaaaaga aaatctaagc tatgacaaac ttgtaattgc tacaggggca gacccaataa
     3001 aaccaccaat acctggaata gatttacctg gagtattctt tatgcgtact ccaaatgatg
     3061 ctataaaact aagagattct attgaaaacg gtattaagag agctgttgtt attggtggag
     3121 gtttcattgg tcttgaaatt gctgaaaatc tttctgcaat gggtatacgt gtaagtgttg
     3181 ttgatatggc tgaacatgtt atgcctggat ttgatactga ttttgctgaa tacgttgaaa
     3241 atcatatggc tgataaaggc ataatgattt ttacaggtga ccaggttgta ggtattgaag
     3301 gtgaaaataa agttgaaaaa cttcgtacta aaaatagagc tattaaaaca gatttagtgg
     3361 ttatgtcagt tggaatccgt cctaacacag gttttctaaa agacacaggt ttagaatttg
     3421 cccctaataa tacaattatt gcagatgaat atatgcttac aaatgataaa gatatatacg
     3481 tagttggaga ctgtgctttt gtaaaaaatt ctttaacaaa taaacctaca tggtcaccta
     3541 tggggtcatc tgcaaatcat gagggtagaa tttgcgcaca aaatatatct ggaaaatcta
     3601 aaacttacaa tggtgttctt ggaacgacag tagtaaaatt acctgagctc aatgcaggaa
     3661 aaacaggtct aagtaaagaa atagctgaaa aagaaggtta taatgtttgc tctgtaacca
     3721 tagctaccga tgataaagct cattattatc caggtgcatc taattttatt ataagaatgg
     3781 ttgctgaaaa aaatactaga aaattattag gtgttcaagt tatgggacct ggagcggttg
     3841 ataaaatagt agatatcgct gctactgcaa taactttagg cgctgacctt tattcattag
     3901 aaagtatgga ccttgcatat gcaccacctt tttcaacagc tattcaccct tttgtagtgg
     3961 cagtaaatgt tttacaaaat aaacttaacg gagaacttga tagtgtttta cttgacgaaa
     4021 ttgaggattt tgacagttgg actaaacttg atgtttcaaa agctccttct attcctagtt
     4081 tacgttatat tcctgttggt gaaattaatg gagaaattga aggtcttgct aaggatgaaa
     4141 aaattcttct tatatgtgct aaaggtaaaa atagctatat ggcgcaaaat cgcttaagac
     4201 gctttggcta tacaaatacg aaagttcttg aaggtggaat actattttat ccaaatctta
     4261 aggtaatgga ggagtaaata tgagtaatgt tacagcagaa caaaaaaagg aagtaaaagg
     4321 tagaggtttt ctaccaagca gggatggaga acattttgca gcacgtataa taactgtaaa
     4381 tggtgtaata aatgcatcac aagcaaaaaa aattgcagaa gctgctgaaa aattcggaaa
     4441 cggtcagtta gcatttacta ctagactaac agtagaatta cctggtgtaa aatttgaaga
     4501 tattgaagct ctaagtgaat ttattgctag cgaaaatcta ataacaggtg gaactggatc
     4561 aagggttcgt cctgttgtgg cttgtaaagg tactgtttgt gttcatggtc tagctgatac
     4621 acaggcactt gctactgaaa tacacgaaga attttacaaa aattggtatg atgtaaaact
     4681 acctcataag ttcaaaattg gtgttggtgg atgccctaat aactgtatta aacctgacct
     4741 aaacgatctt ggaattgttg gtcaacgtgt acctgactat gattcagagc tttgtgttgg
     4801 ttgtaaaaaa tgtgctgttg tagaggtctg tcctgttaaa gcagctaaat taacagataa
     4861 aggtaaactt gaaatagaca gtaatctatg taataactgt ggtaagtgta tagaaagttg
     4921 taattttgat agtattgaag aaaaagaatc tggttataaa gtctatatag gagggaaatg
     4981 gggaaaatca gttcgtcctg gaacacagat tgatagatta ttttcaaaag aggaacttat
     5041 gactttaatt gaaaaagcaa ttcttttatt tagagaacag ggtaacacag gtgaacgttt
     5101 tggaataact atcgatagaa ttggtgctga taaatttata gaaatgcttt tatcagatga
     5161 agttttggaa agaaaagaag agatactaga ggctctttta caccttacag gtggagctgt
     5221 atgctaacaa taaatatttt aattgtacag ataacctatg gaaaaaattt tagttaagaa
     5281 taattaaaat tctatatatg atatatgata aatgaggcta tctcaaaaca cagatacgcc
     5341 tctttttcat tgttgaaatg ataaagtata tttgagttta tacaaaaaag agtgtctcgt
     5401 gaattataaa attcattttg aggcacccac attattttat aataattgct tattaatttt
     5461 attttaacta tataaaaagt acaaaaagta agaatatcca atattctaaa attaattttg
     5521 aattgtattg attgaaaagt ttactattcc actatttcca tcttgattta ctactagata
     5581 ccatgttcca gtattaggaa ctctcattct acatggattt ttctttgcaa atccaccata
     5641 gtaatcataa tttctttgat ttttaaattt cacataattt atatggtcta acaatattac
     5701 attggcttgt ttctctaaat ttacctctac aatatcccct ttttttaagt tcatcttact
     5761 ataactatat tccattgaat tactcctcca gtatcgaata atctttgtta tttactcatc
     5821 tcaaatatgt aaaaattcat ttaaaatatt acaaatacta tataattaga ctattatcca
     5881 gttttaacct tatttactac aatccaaagg ttattaaaaa tattattggc aacaacttct
     5941 ccatcccata caactctttc ttctttattt tctaataaag catagtaccc agagtcagaa
     6001 ctttcatctt tatagactgt aacataaaac tcttcatcac tatattcagg tatattttta
     6061 aagtcttcag atattttaca tgttaatata tacccttcta attcttcatc tataaacaaa
     6121 gcaattcctc caatctctat atttttacta attataccat attatgtcat aaacttttta
     6181 tttcattaca aatttctatt ttttctttac tttataaata aattatataa attttcactt
     6241 ttta
//
LOCUS       DAEPOG010000199         6243 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEPOG010000199 DAEPOG010000000
VERSION     DAEPOG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.199"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..114)
                     /locus_tag="KR432_003272"
     CDS             complement(<1..114)
                     /locus_tag="KR432_003272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0641782.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRS"
     gene            complement(431..940)
                     /locus_tag="KR432_003273"
     CDS             complement(431..940)
                     /locus_tag="KR432_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0641783.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(942..1589)
                     /locus_tag="KR432_003274"
     CDS             complement(942..1589)
                     /locus_tag="KR432_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641784.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1603..2775)
                     /locus_tag="KR432_003275"
     CDS             complement(1603..2775)
                     /locus_tag="KR432_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0641785.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            3043..4419
                     /locus_tag="KR432_003276"
     CDS             3043..4419
                     /locus_tag="KR432_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641786.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4670..5503)
                     /locus_tag="KR432_003277"
     CDS             complement(4670..5503)
                     /locus_tag="KR432_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641787.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5832..>6243)
                     /locus_tag="KR432_003278"
     CDS             complement(5832..>6243)
                     /locus_tag="KR432_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0641788.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2167 a         1060 c          673 g         2343 t
ORIGIN      
        1 gcttcttata ttcagcatct cagcaatttt gtatgatgag tatccttctt ttatatataa
       61 atttacagct tttagtttaa gttctttgct atatttttta tgttttttag acatagaaaa
      121 acccccttag agtatacgct ttttagactt attataatag ccttttattt aaatgtctac
      181 tataagggga ttatactaaa atagagataa gcctctcttt cattgttgaa attatgaaaa
      241 tatacttgat ttaataccaa aagagtatct catgaactaa aaagttcatt ttgagatact
      301 ctcttctttt ttaattaata tacatattaa ttactatatt agtttattac tatatatcaa
      361 ttactattca ttaattacta ttcatcaatt cttatatatc atttataatt aggttataat
      421 tttatgttat ttacattaat cccataacag tgagtaaaac tcttactatc aacatcccca
      481 ctattgcatc aaatatagga actgccaaca gtaacattct atacttagta ctggcatttg
      541 ctacaagcac tattctggca taatgaccaa ctagagtacc cattaacata catggcataa
      601 ttaatatggt tgcttgtggt gcagtgatta gaccttgtgc aaataagttt gcagctgtag
      661 ctgctcctgc tgccttagca aagaatgctg ctatcaaaac tacaacagat tccccaggta
      721 gtccaaaaat acccattact ggaccaaaaa tatttcctaa aatatcaact aaaccagtta
      781 attgtaaaaa ctgcactatt acataaccca ttatcatggc tggtaatatc tgttcaacac
      841 ctatgtaaaa accttttttt gctcccccca taaatatctc aacagcagtt ttcttaggtt
      901 tactatttac aattgcattt ccagtatttt gttgactcat actacacagc ctccttagcc
      961 ttattctttt tatttcttac acctatgatt aatctaacta gattagcacc aagtatttta
     1021 cagaatatct ctataaggat tattggacct aatgccaata atgctattgg taaaagtgga
     1081 gcttgagtat ttattgtgtt cagtataaca gctgaaccag catattgata tgctacaaat
     1141 atagttcttt catcatctgt tatttcccca ctttcaaata attctttcgt cattattgag
     1201 gctacatctg aactagtaaa tgaagataca aatgctattc ctgctattcc aggtatacca
     1261 agcagaggtc taagtaatgg attaaatatc tttgatgctg ctttcattcc tcccatagat
     1321 tcaactacat ctataagacc tacagcaaaa cagacagttg gaatcaaagt cagagcaaat
     1381 aagaaaccat cttttgctcc cgttcctcct ttacccataa agtctacacc atctgcaata
     1441 gtaccaaatg aacctgtcag attagcaaaa tctaatgctt taaggaagcc ttgactatct
     1501 ttaaatactc ctgaaaataa tactatcaat acaaataaag ataaataccc tttccatgtt
     1561 accttattat ctccagctgt tacatccata taaaatcccc ctttatacct caaatttatc
     1621 taagtttaaa ataactgtag aaattaattt caccctctca aacatagaat ctactactgc
     1681 atattctctt gatgtgtggt tccattctcc tcttacacca aatgaacaga ttgtaggaac
     1741 gtttgcaata gttaaataac ttgcatctga actacctcca agtctcttag catttaattc
     1801 tccaaatcca ttttctcttg acacttcatt tacaaatgta tgaaatctca taacatcttc
     1861 tgtagtttca aatggcatca tttcacttat aaaattaact tctgtagaag ttccttctat
     1921 gtaagtttct ttacatatct ctctaacttg ttctttaaca ttctccattt cttctacttt
     1981 ttcaaatcta aggtctactt ttatactaca atcttctgga atagaatttt ccactcttcc
     2041 acccttaatc acacttacac ttactgtagt tccaacatct aaattagtca atttttgtat
     2101 gcgtagtatc ttattagcca tttcttctat agcatttctt ccaccttcaa aatcattacc
     2161 tgcatgtgtt tctacacctt ttacatgtat attacatcct attcttcctt ttctacctat
     2221 acataaacta ttatctacta atccagtttc catattaaaa gcacaaagag caccttttgc
     2281 ttccctcaat ataacatctg caccagttga atctttatga cctatctctt catctccaga
     2341 aaatactatt tttattggtc tttctttgta tcctatttta tttaaagctt taataacgta
     2401 taaagagatt attatacccc ccttcatatc taaaacacct gggccatatg cttttccttc
     2461 tattatttta aatggattct ctccaaatgt acctgtttca aagacagtat ccatgtgtcc
     2521 tgaaaaaatt atcggctttt tatctatatt agaaccaagt gtacctatca atgtattacc
     2581 attttcccca acatctacta gttcacaatc caaaccttct ttttcaaatt caagctttaa
     2641 tctctcagct aacttgttta cactttcttt acaatgtgta taactttcta tattaacaat
     2701 ctctttccat aatgaaacta tttcttctct attctcatca acaaacttag aaatttcatt
     2761 taaatatgta ttcataatct tctcctttat tttcaaataa aatgtatttc tttcctaaaa
     2821 tctaagttta ttttaggaaa tcttaaaaga ctagtcctta attacaaaat agcacaatta
     2881 ttcttgccag tcaacatatt tttcagtctt ttttatattt tctgattttt ctttcaatta
     2941 tccttttgta aaataaattg acgcgtttta tctactatta taaaatgagg ttataaattg
     3001 attgattagt tacaatactc tcgaaatttt aaaggaggtt ttatgaaata taaaattggt
     3061 tttttgacaa catcttattt tatgtcagat aagtttaaac aagctcttaa agaattaaat
     3121 gatacttgtg aaattacatt tattgagctt aaaggggaga atccttttaa taatttacct
     3181 aatatatact atgagcaagt agataatttt gatggatttg tatgcagtgg tatagttcca
     3241 tatagtgaac ttataataaa tataaaagac ataaaagttc cattaaattt tttaaagctt
     3301 gatgaaagag atttttataa atacttattc aaattattaa atagccaaaa agatattgac
     3361 tttacaaaat catttatgga ttttttaaga gaggacaaca attattatga tatatattca
     3421 ttaatagact ctaataaatg tccatataca attaaagatt ttaatatccc tcaacctgta
     3481 tatgacttta aaaaattaaa tgataaatta ttggaaatac atttagactt attgcgagat
     3541 aataagatta acctttcttt tactagaaat tacatcatta gttgtgaact taataagctt
     3601 ggatatgatt gtatttacat ggttccatct acagaatcaa tcctgaatac atttaaatct
     3661 ctggtgaatg aaataacatt acaaaactta gacaaaaata aatctgcaac ttgtatagtt
     3721 acagtaaata gttcagagta cataaatgaa gatttgatat ttaaaaatga tgagatacaa
     3781 aatcaaatat ataacactat attaaattct ctatcaagac atggattcta tggagttcag
     3841 gtcaaaaaaa atgatatgaa aatagagata cacactacta aagaaatatt aaacaatatg
     3901 accaataact atacagattt ttttatttca gaagatttaa aagaaattaa atatagctta
     3961 aatataggtt ggggcattgg aaatacaaat attcatgcag agcaaaatgc tcgtcaggct
     4021 aatggtaaat ctgcttcttt gaatggaaac tgtactttta tagtaaatga tactaatgat
     4081 acaataggcc ctttatattc aaataaaaat tctaataata ttgaagatag ctcaatagcc
     4141 aataaagttg cttcttttat acctctatct aatacaaatg tatctaaaat catgtgtatg
     4201 attaatgata gaaattcaaa taatgtgtca gcagaaatat tagcagacta tatgaacata
     4261 actcttagaa gtgctaatag aatattgagt atcttatata aagcaggaat tgcaactatt
     4321 gtaaatacaa aactcgataa tcaaagagga aggccaaaaa aaatatataa agttgatttc
     4381 ttatcttttc taaataagat gcagaaatta aattcttaat atttaatttt ttaataaaag
     4441 caatgtattt tctctaattt taaagaatca aaatgtttaa attttattag aatatacaac
     4501 tttttacttt aaaataccta aaccttttat cagtgaaata aaccataata tatcgtagct
     4561 tattagaaaa acaaaataat ttcatataca aataataaaa tagccactac aatccataat
     4621 ttgcagtggc tactttttac actatcactt attacttaat ttttaaaaat taattgtatt
     4681 ttttaaataa tttatacaca cctattatca gtaaaacaat aaatacaagt atatctataa
     4741 aaataattat tgtaacatgt ctggcaatat tgtaaataaa aagtatagag aatatttcta
     4801 tagacaacgc tacaaaacac ataaaattcg ttaaataatc tgcaatagta tcactataaa
     4861 aagcatgtat ttgtaaccat ctccatataa atggacgtaa aaggattctg aatacaacac
     4921 aaatgacagg gtaggcataa ataaattttt tatctacata attagctaca ccattattcc
     4981 attggatagg aatttgaggt ggcaactttg gaaatactgt tattgcaatc aacaaacaag
     5041 caatagtaat aattccccag gtaattttcc ctccccatat atataagaaa cttacagagt
     5101 caagtttaaa aacaggtttg tttttattcc agtattctgg caactcagtt atgtattgct
     5161 ctactttcaa attcaaataa cttagctcat cagattctaa cattttatta cacatagttt
     5221 ttgcattttc taagtctgat attttagttt cgagaacttt ctcctgtttc tctacaactt
     5281 caactaaaga tactttctgt aatataatat tacggatatc atcaatagta attcctagtg
     5341 tacgtaaata tgcaattttt ttgatgtcct ctacattttc atctgaatat tctctatagc
     5401 cattacgctc attacgaagg ggtttaatta aattctcgtt ctcataaaaa cgaatattag
     5461 agcgtgtcaa tcctgttttt tcttcaactt ctttaattgt cattttaaac actacctctc
     5521 tattctatta atttacaact ataatatagt cttaaatatt ttcactctat aaaattgtat
     5581 atttagtata tcttaaaatc aagttaatta tcttttataa attttcatac aataatatct
     5641 cctttcaata atatctcttt ttctatattt aaaactatct tattttctat aaaataatgt
     5701 ataaacaagg tttacagtca agtattttta ttattttttt gatttcataa aaaaaagcat
     5761 agcaaatcat ttgctatact tttttactaa attatttatt ctatatctta tatgaactta
     5821 ttgtttaact actacacaat actagcttta atcatcatat tttttgcaaa catactggct
     5881 tcatatattt cttgttcatc tggatgactt tgagattcct catggttatt taattgttgg
     5941 cgaatatgtt cgttgtcagg agaaagtttt aacatctctt tatattttgc tttcaatcct
     6001 tctgctatct taccttgaca tatgaatgaa cctaatattt cattatcttt tgggataatt
     6061 gattctatct tttctattat actagctcca taacttcctt ttttacttgc tcccattgtt
     6121 ccaaatagtg ctatcttttt attgtgaata ttttcaagca cttgttttga atttttatca
     6181 caaattccct tgtccaccca atagcctaag aatactatat cataattttc atagtcaata
     6241 cca
//
LOCUS       DAEPOG010000200         6207 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEPOG010000200 DAEPOG010000000
VERSION     DAEPOG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.200"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            67..1386
                     /locus_tag="KR432_003279"
     CDS             67..1386
                     /locus_tag="KR432_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0641789.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(1461..1889)
                     /locus_tag="KR432_003280"
     CDS             complement(1461..1889)
                     /locus_tag="KR432_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641790.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(1980..3356)
                     /gene="hydG"
                     /locus_tag="KR432_003281"
     CDS             complement(1980..3356)
                     /gene="hydG"
                     /locus_tag="KR432_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBH0641791.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3357..4442)
                     /gene="hydE"
                     /locus_tag="KR432_003282"
     CDS             complement(3357..4442)
                     /gene="hydE"
                     /locus_tag="KR432_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBH0641792.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(4478..5686)
                     /gene="hydF"
                     /locus_tag="KR432_003283"
     CDS             complement(4478..5686)
                     /gene="hydF"
                     /locus_tag="KR432_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBH0641793.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(5701..5952)
                     /locus_tag="KR432_003284"
     CDS             complement(5701..5952)
                     /locus_tag="KR432_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBH0641794.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         2080 a          964 c          903 g         2260 t
ORIGIN      
        1 agaatatctt tataaatcgc aataatttta gaacataaat ttatttaaag aggggatgat
       61 ttgccaatga gcgagataac tagtcaaatg ataagtacag aagaaaaaaa atatgttgct
      121 aaaactcaaa agatacctta ttatccagta gcatttaaat caggtgatgg tgcaatgcta
      181 tatgattatg aaggtaatga atatgtagat tttttagcaa gtgcaggctc tgcaaatgta
      241 ggtcatggta ataaggaaat ctcacaagca gttaaagaac aaatggatga tataacacaa
      301 tatacacttg catatttcca tagtgatcct cctgtaaaat tagctgagaa gcttgtggaa
      361 atagcccctg gagataatga taaaaaagta ctatacagtg caactggttc tgcttgtata
      421 gatgctgcta taaaattagc aagagggtat actggaagaa caaagataat atctatgtgt
      481 gaatcttatc atggaagtac ttatggagct atatctatat ctgctttaag tacaaatatg
      541 agaagaaaaa tgggtccatt gttaccagaa gtttatcact tccattatcc tgataaaaat
      601 agaacagcta aagaatgttt agatgagata gaatatgctt ttgcacacta tctaccagca
      661 gaagaggtag ctgcaatatt tatagagcca attgcaggag atgcaggaat aatagtgcct
      721 ccagtagagt gggtacaagg tttaagtaaa atatgtaaag aaaatggaat attacttgta
      781 agtgatgaaa tacaacaagg tatgggaaga acaggtaagt ggtttggtat agaaaatttt
      841 ggtgttgaag cagaccttat agttcttgga aaatctgttg gaggtggatt acctttagga
      901 gcagttgttg gaagaacaga aataatgcaa agtcttgatg caccagcaca tttatttact
      961 ttagctggaa atacaacagt gtgtgtcgct gctttaaaat ctatagagat aattgaaaaa
     1021 gaaaacttac ttcaaaagag tatagaaatg ggagattata ttaaagcagg atttgaaaaa
     1081 ctaaaagaaa aatacgatat aattggagaa ataagaggaa tagggttatc tataggagtt
     1141 gatatagtta aaggaaaagg ttctaatgaa aaacatccag atgctacagc taagatatgt
     1201 tatagatgta tacaaactgg attaataatg atattcttag gacaatctac tttaagagta
     1261 caaccacctc tagtaataac taaggagcaa gtagataaag caatgagcat catagattct
     1321 gcaatagatg attatttaaa tggtagaatt ggtgatgaag tatatgaagt aactcaaggt
     1381 tggtaaaata aatattaaat gaaaaaaaga gttatctcaa ttttagaaag taatttctga
     1441 aatgagataa ctcttttaag ttacccacaa ataagaaaat tgtttgattc attaaagact
     1501 atatttcgga tgtcgttttt gtgagtatca gagacagtat tacgagaata attaccttta
     1561 ttcgtactat ctgttaattt agttataatt aagatagcta aaagaataag aattactgtg
     1621 tataaagcaa taacatatgg ttttctttta tatttgtatt tgttattgaa aaaagaatgg
     1681 ccataattat ttttatttac tttagatgta tttgtattct ttttcttgtt acctttcttt
     1741 ttatcatcct tttttctatt tttagggtaa ggaatgactt tgtgtgtatt atcaagtttg
     1801 atatttttat ttttttgaat attgttttcc aggctagcct ttttttctcc aaattgaata
     1861 gttttattgg tattgttgtt atctttcaat gttaatctcc ctcccttaaa ctttcccttt
     1921 agataatatt actactaacc tatggaatat acaataaata atatataagt aataaaattt
     1981 tataggtata aatctctttc tccacgctct aaggcagtca tattgttaga tactatgttt
     2041 cttatatctt ctctttctat cttacctatt tcttctgcga taagtttttt acccataatt
     2101 ttaagttctt catctccata atcgattaaa aattcattta atgtagtaag agcatttggc
     2161 gtacatacat tatggatttg tcgactttta gctaagctca tgaatctatc gccagttcta
     2221 cctttacgat aacaagctgt acaataacta ggtatatatc catcagtaat cagttcttta
     2281 agcacctcaa cagggcttct atggtctcca acttcgaatt gttctgtatt gtcaccatct
     2341 tcatatgctt tatatcctcc aacacctgtt agagaacctg cactaatttg tgatacacca
     2401 tattttagta actcatttct catttcagca gtttctctag tggacattat gattccagta
     2461 aaaggaacag ccaatctagt tatagctact atttttttga acatatcgtc agaaactaag
     2521 tgaggaaaat cttctaagct cataccttca gcttttttca atctagggaa agatatagta
     2581 tggaaaccta caccaaattt ttcctctaag tgttcgttgt gcatcataag tcctagaact
     2641 tcaaatttag ggtcagataa accaaataaa actcctgcac caacatcatc aacaccagct
     2701 tccatagctc tatcaaaagc tgttaaatgg taataatagt cattttttat agattgacca
     2761 tgcattttta taaatgttgg tttgtgataa gtttcttgga ataatatata agttcctatt
     2821 cctttttctt ttagtaattt atactcatct acacttgtag ctgctatgtt aacgtttacc
     2881 cttcttatat ttccgttttc attatatgtt gaataaattg catctaagca atctaagatg
     2941 taatttatat cacaattttt agggtcttca ccagcttcta gagcaagtct tttatgaccc
     3001 attttttcta atattttaac ctcttccttt atctcttcca tggtaagttt ttttctttta
     3061 aatttgttgc atttactaaa accgcaataa acacattcat ttacacagta gtttgataca
     3121 taaagagggg caaataaaac cactctatta ccatatattt cattttttat ttgcccagct
     3181 atactaaata atctatctag ttgtttttta tcttctactt caagaagtgt agctatatct
     3241 ttataactaa gtttttccct tctatcagcc ttatcaagta ccttttcaat atcatcagaa
     3301 gttgaatttt ttgcatcttc taataaagaa ttaataagtt catggtttat gaacatttac
     3361 aaacgcctcc attcaatatt atcaccgcga gaatagtcaa cttcaagtcc taatgacttg
     3421 atgttttctt ctaaactttg gtgatattca caagcttctt gaccagtaaa ggctttgtta
     3481 tcgtaaagag aatattgttt tctaaattct tgaggagaaa ggtttggcat aattacattg
     3541 caaccagcta aaagacctgc atttctacca ctagggttta tagatgccaa ggctgttgta
     3601 gcgggtaata aaacttttgg aagtagaagt cttgtaattg aaagcataag taatgtcttc
     3661 tctaaatctc catgtttata atccttaaaa attgtatcat ggtgtggaat aaaaggtcct
     3721 attccaacca tatgaggatt taactctttt aggtataata aatcacgaac aaggtctttg
     3781 ttgttctgaa atggtgattc aaccataaat ccagcaccta cctgataacc aatttcttta
     3841 aggtttttta ggcattcttt tctagttcta agctcaatgt ttggcggatg taatttttta
     3901 taatgactgt ctgtagcagt ttcatgtctt aaaagatatc tatctgcacc acataaaaag
     3961 tattttttat acgaatcata actcttttct cctagtgaca gagttatggc acaatcctca
     4021 tatttatttt ttatacttga aacaatttca catattttat catcagtaaa atatgcatct
     4081 tcaccacctt gcaaaacgaa tgttctgtat cctattttat aacctatatc acaacattca
     4141 agtatttcat caggtgaaag tctgtatctt atgatatttt tattagaaga ttgtattcca
     4201 caatagtagc aattattttt acaatagttt gtaagctcta taagtcctct caagtagact
     4261 ctatttccat aatgtttatt tctcatgtcg ctagcttgtt tataaagata gtttcttaaa
     4321 ttttgttcga agttatattc ctcattgtcg atattttcta ttaaatatag tagttcatca
     4381 aaacttaatg aattaatgct gtaaagttta tctataaagt atttaatctt atttttagtc
     4441 atattcaaat atgtaattat taagtatagt taaattttta ataattgaaa agttcaagtg
     4501 atctttccaa aatacctttt acatatgcta ttaagatacc atagtttaca ataggtacat
     4561 tgaaagattt tgccttttct attctagata acattccagc tctattcatc atacaagctc
     4621 cacagtgaac tacaagggca tatttattta tatcttcagt gaaggatact ccagaactaa
     4681 attcaaaagt aatatttttg ccagttttct ttctaatcat attaggaatt tttactgttc
     4741 caatatcatc agtttgtcta tgatgggtac atccttcagc catcaagatt ttatcaccat
     4801 cttttaaatt ttctaaagca taagctccat taatcaattc tttaaggtct cccttttgtc
     4861 tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt tggtgtatct ttatttactt
     4921 gtggaaatac ttgagagtca gtaataacaa gttttggttt tttgcctaaa ttagatagag
     4981 tttcttttaa actatcttct tttgtaacta tagaaattgc accattatcc aaaatatctc
     5041 ttataacttg ctgttgagga agtataagtc taccttttgg agctgcttta tctattggaa
     5101 caactaaaac aactaaatca tttggttcaa ttaaatctga taccaatttg aactcagtgc
     5161 tatccttagg caaaactttt attatttcat tttttaaatt ttcaattcca attttatctg
     5221 ttgatgagac agaaacaact ggacatttaa ctttattttt agttaagttt aaaatttctg
     5281 attgattctt aatagtatca attttgttga gaattagaat atgagggata ttcttatcat
     5341 taaacttttc aattagtgac aaatcttctt gtgatatacc aatttggcaa tccactacta
     5401 gtaaagctat atctgtcttt tctaaaacat ctagtgattt acctattcta agttcaccaa
     5461 gttcaccaac atcatcaaga ccagcagtat ctataagaac acaaggtcct ataggaagga
     5521 tttccattgg tctaaatact gggtctgtag tagttccagc aatatctgag actattgcag
     5581 cactttgatt tgttatagca tttattatgc tagatttacc tgcatttctt ttaccaaaga
     5641 gacctatatg tactcttacc gattgtggag tagaatttag actcatttgt actcaccacc
     5701 ttaaacttct ttttttgata tactagtttt tacagataca tgaggtatgt tgcccaattt
     5761 accagtcaaa ctattaatag tatccatttc gccaacaact gctatacata cgattgagac
     5821 accttcttct tcgcaaggaa tgcccattct tcctttaata atacctttaa aacctgatac
     5881 aacctcattg aatttaaatt gacattcttt tggctcttcc aaaatagcac ttataactgc
     5941 aacctttttc ataaatatcc tcctaactca gaaaatatgt cttaaacaaa tccaaaatca
     6001 aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa agttattaat tacacaagta
     6061 taatgtgcta tattaaacta tttccttaca gattagataa ctcttttctg gtagggttag
     6121 cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa tttttataca atttatataa
     6181 attataataa aaattaacta aagaaaa
//
LOCUS       DAEPOG010000201         6148 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEPOG010000201 DAEPOG010000000
VERSION     DAEPOG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.201"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..367
                     /locus_tag="KR432_003285"
     CDS             <1..367
                     /locus_tag="KR432_003285"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0641795.1"
                     /translation="IRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASSKSKKKSNV
                     REIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIKYMLESKDR
                     CQSSDTAPAKGLCLWKVTY"
     misc_binding    478..685
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            938..2137
                     /locus_tag="KR432_003286"
     CDS             938..2137
                     /locus_tag="KR432_003286"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH0641796.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            2384..3430
                     /locus_tag="KR432_003287"
     CDS             2384..3430
                     /locus_tag="KR432_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBH0641797.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            3538..4701
                     /locus_tag="KR432_003288"
     CDS             3538..4701
                     /locus_tag="KR432_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641798.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(4790..5170)
                     /locus_tag="KR432_003289"
     CDS             complement(4790..5170)
                     /locus_tag="KR432_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBH0641799.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(5173..5832)
                     /locus_tag="KR432_003290"
     CDS             complement(5173..5832)
                     /locus_tag="KR432_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH0641800.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         2400 a          656 c         1006 g         2086 t
ORIGIN      
        1 tataagaaaa tatgctactc atgtaagtaa aaaattagat ttagatagaa tgagaaaagc
       61 aagtgagtat ttagtaggag aacatgattt tactagtttt gcttcttcta aatctaagaa
      121 aaaatcaaat gtaagagaaa tttattctat aaatataaaa gaagatgata atgttataga
      181 aatatatgta gaaggaaatg gatttttgta caacatggta aggataatag taggtgcttt
      241 gatagatgtt ggtctaaaaa gaagagctcc acaagatatt aagtatatgc ttgaatcaaa
      301 agatagatgt caatcatcag atactgcacc agctaaagga ctatgtcttt ggaaagtaac
      361 atattagtca attatataaa actctcttta aaataaaaat aaaataaatt atatagttga
      421 cataaatgga aaaataaact attatatatt ttataagaga gatataaaac tttatattta
      481 aatcctgtga gaaagagagt aacttctatg tgttttatag agagctgagg atggtggaag
      541 ctcagcaaat aagtattagt gaaaagaact ttggaggata ctaattgaat ttagtatgtt
      601 agtaggtaat ctcgccttaa gagaaagagt ggataagttt atcttatcaa ttagagtggt
      661 aacgcggata tatttcgtct cttggttttt ccaagaggcg ttttttaata tatttaatta
      721 ttagtgttta tctggtatgt tgaacattta ctaattgatt agctaagttt atcaataaga
      781 gtggtaccgc ggatatttcg cctcttagta ttttaaaata ctaaggggtt tttttagtat
      841 taaaatgatt ttatgtgagt ctaaattctc agagtcattc aatattaaga atttctataa
      901 aataaaaatt ataatttaag attgggagga attaaaaatg aaggaaaaag tagtattagc
      961 atattcagga ggacttgaca catcaatcat aataccttgg ttaaaggaaa attatgaaga
     1021 tatagatgtt atagctgttt gtggaaatgt aggtcaggaa gataaaatgg aagatgtata
     1081 tgaaaaagct ttacaaagtg gagcatcaaa ggcatatgtg gatgatataa gtgaagaatt
     1141 tgtaactgaa actatattta aagccgtaaa agcagaggct aaatatgaag gaaaatactt
     1201 attaggaact tctttagcaa gacctataat agctaaaaaa ttagttgaag ttgctcataa
     1261 agaaggtgca aaatatatat gccatggatg tacaggaaaa ggtaatgacc aagttagatt
     1321 tgaagctaca attgcagctc ttgacccgac tataaaagtt attgcacctt ggagaatttg
     1381 ggatataaaa tcaagagaag atgctataga ctatgctgaa aaacataata taaaggtaac
     1441 agcaactaaa gctaaaatat attcagtaga tgctaacctt tggcatgttt caacagaagg
     1501 tggagatata gagcacctag aaaatgaaca taaaaaagat gtttataaac aatgtgtaga
     1561 cccagaagat gcatgtgatg tagctgaata tgtagaagtt tactttgaaa aaggtgtacc
     1621 taaaaaagta aatggcgaag aattatctcc agttgcactg attcataagt taaatgagtt
     1681 gggttgtaaa catggaattg gtgttataga tatagttgag aatagacttg ttggtatgaa
     1741 atcaagaggt atatatgaga ctccaggagg aacaatcctt tatgaagcac ataatatttt
     1801 agaaagtgct actttagaca aagatacatt acatttcaaa caaatggtat cttataaata
     1861 cggagaactt atttacaatg gactatggta ttgtaaatta agagaatcta tagatgcatt
     1921 tatggaacaa actcaagata atgttacagg aacagtaaaa gttaaacttt acaaaggaaa
     1981 tataaaacct gctggaatat ttactgaaaa tgctctttat gatgaaggaa tatcttcttt
     2041 tggaaatagt gaactttatg accacaaaga tgcagaagga tttattaatt tattcacttt
     2101 accattaaaa atcagagcta tgaaagctgg aaaataataa aataaattat ttaagagtgt
     2161 ataaaaacta tttttaggtt tttatatgct ctttttttat ttgaatttat taaattatac
     2221 agtaaatagt ataaaaaaat atattatagt tccaaatgac ttaataatgt gaaaatataa
     2281 gacctaaaat ataaatttta taaatacaat agttattgac tctctcattg taagagggtt
     2341 tatactgaat ctaatgaaaa tttatagtga ggagatagat tatatgagaa aaataattga
     2401 aattaaacat cattcacatg attatgagtg tatgtggaat ggtatagaag atttatatat
     2461 aaataaaacc ggtgaatgcc ttccaaacga ttttttcttt ttactgtcag gttttggttc
     2521 attttgttat atgaaaacaa ataaagctga tttgaaaaga atggtagctt tgggagatgg
     2581 aaggacaaaa cagatgtata aatttttagc accaattgtt ggatttgaat acaaacatca
     2641 tgagtttaaa aagtatgagc aagcattaaa gaaagcaaaa tctgaaattg atggtggata
     2701 tccagttatt ttaggagctt tagatatgta ttatctacca tattataaga agctatatca
     2761 taaagaacac attccttttc attatatatt aatggtagga tatgatgatg aaagaatata
     2821 tctgtatgat tgtggtagaa cagaattatt acatctttct tatgataaat tacgtgaaag
     2881 catgaattgc aattcccctg gattaagcaa agaaaatact atttgcacaa taagaatgaa
     2941 tacttcaaag aataaatata acattgctag agaagctatt gctataaagc gtgatatgtt
     3001 tttaaatcca tctaaaggat ttttgggata taaaggtttt gaaaagttta ttaaagagtt
     3061 gccacagtgg aacactgaat tggataaaga tgattatgat aagattttga ccaatatggt
     3121 tatgtttttt ggaacagtac caacaattcc aaatgcaatc aaaggaatta aagaaccaga
     3181 tactgttcaa tttaaaggtg gatttgataa aatgagtaga atgctcaaca gtattgggga
     3241 ggaatacaaa aacgactctt ttatacaagt agctgatatt tttgataaaa ctgctattat
     3301 aattgagaaa atatccaata ttataataga ttatttaact caaacatgtg atgatacaga
     3361 acaacttccg ttattgttct ctgaggtctt agagcatatg aaaacaggat atttaatctt
     3421 ggatgtataa aaaatatatt gttgacccta acattacgta atagtgtaat atgaaaatat
     3481 agcttgaata tattagtagg tattgatggc tgtaaaatta aaatttgagg tgtggatatg
     3541 agaacagtga aacaagtttc tgatttaact ggaataagtg tgagggcatt acattattat
     3601 gatgagatag gtcttttaaa accaaataaa attacagatg caggatatag actttatgat
     3661 gatgaatcta ttaaaactct tcaacaaatt ttatttttta aggagattga tattccttta
     3721 agagaggtta aggaaataat gtcaagccaa tattttgata aggttgaagc tctaaaaaat
     3781 caaaaaaaat tacttatttt aaaacgaaag agactggatg aattgataga gcttataaat
     3841 caaactctaa gaggagaagg aaatattgat tttaaagagt ttgatatgag cgagtatttt
     3901 aatgttttag aagaatttaa aagagaacat aggaataaga taattaaaat ttatggtagt
     3961 gtagaaaaat acaatgaata tatagaaaga gttaagtcta atgaagagaa aattgctaaa
     4021 atggctataa aacaatatgg aactattgag aagtttgccc aagctataaa aacaaatttt
     4081 agcagtgata tattgaattt aggagaaaaa tttgatagat ataaaaatga ttgtttaaaa
     4141 gaaaaacatc caaaattaaa agaattatat agaaagcttg tagaagattt aagtaagaat
     4201 ccttcttcaa cagaccttca agaaatagca aaagagataa cagatatatc taaaaaagat
     4261 tatgaaattt tctctatgta tactggtgat gacaactggt attatatgat tcaaaattat
     4321 ctagtaaatc ctatgtggat agaagaagta gataaaaagt atggaagtgg agcaggcaag
     4381 tttattggac aagtactaaa gacttatcta agagatagaa aaccaaagat aaatacattg
     4441 tatgaaaaac ttgtagaaga tttgagtaga gattgttctt caagggaagt tcaaagcatt
     4501 gttgaagaaa tagacaatga aatgaaaaga agtaatgaat tttataaaat agacaatggt
     4561 gaaagatact ttgattatat gtcagagctt tatttacaag attctaacta tataaaagta
     4621 actgataaga attatggtga tggtgcatct aagtttattg gagaagcttt taagatttat
     4681 tttgataata ataattgttg aaaatttgga agtaaaaaat atgtaagttt tataaaagca
     4741 caaaaataag gatgtagatt agctccatct atatccttat ttaaagttat taaaatatat
     4801 agtttacatc aaacttaaca ctttcaataa tatttcccaa atatgttttt atactttcaa
     4861 cttcttctaa agattttaat ttattttcag tataaattgc tttgtccaga atattaaacc
     4921 ttaatcctat ttcatcaaag tactcatgat ttaaaacttt gtaccaatgg tttttttctt
     4981 tattccaact cgtagatatt gaatcagctt ctttttgagc tttttcccaa tcttcatttt
     5041 ctatagattt ttctaaaaca gatatattgt ctttatatcc ttctgtaaaa tcatatagtt
     5101 tattgttaac gtaaaatcca aataatacaa ataagatagt ccatagtata acaaatgttg
     5161 tagatttcaa tattaatcac cctttttatt ttgaataaat attttgtcat tttcatctaa
     5221 aacacaaata agaacatctt taaaagtttc tatgtgatta gattttaaaa tacccattaa
     5281 ccagttagta tctttttgag ctatgtctag atttttattt attattctac catccagtat
     5341 aagtgggata ggtaaatgat taaaagctct tggaggagta ctattgtagc ttgatgctgg
     5401 tacaacactt agattaccat cagtctccaa aatagcatat tgtacatctt ttaggttgaa
     5461 gtatccttga atcctaagtt gttctaataa ttcatctata ctaattcttt cttttttaag
     5521 ctctttatat acaatttttc ctttatctat taatacagaa ggtttacctg ataaaaatga
     5581 acttaatttt ctgcttttta atgataagaa tgaaataaga gtctgcatga ttacaagacc
     5641 agatattgct gcaacaccat ttatgattgg tatattatta ttttccattg ggacagatgc
     5701 aacttctgca attaatagtg ttataactaa atcaaaacag ttcatttcag ctatttcacc
     5761 ttttcccatg actctaagtg caattagcac tgttatgtat aatataatac ttcttattaa
     5821 tactactgtc atatctatca attactcctt taaaaaatat ttacacttag ttagtatatc
     5881 ctatataaaa aatttaattc aaagtataaa aaaccttaaa taattggatt caactattta
     5941 aggttttttt gataatttta atattaaatt ttttatggat tttctgttac ttttatttat
     6001 tttaataaca gattcaacaa aactcttttt atctcctaat tttaagataa aatcaggata
     6061 ttcttcaaat tcattgagtt ttattaattt gaaagaagaa gatagatttt taaagttaat
     6121 gattttagag ttattcacta atacactt
//
LOCUS       DAEPOG010000202         6128 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEPOG010000202 DAEPOG010000000
VERSION     DAEPOG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.202"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            138..1619
                     /locus_tag="KR432_003291"
     CDS             138..1619
                     /locus_tag="KR432_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0641801.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1652..5089
                     /locus_tag="KR432_003292"
     CDS             1652..5089
                     /locus_tag="KR432_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0641802.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5107..5853
                     /locus_tag="KR432_003293"
     CDS             5107..5853
                     /locus_tag="KR432_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBH0641803.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5802..6122)
                     /locus_tag="KR432_003294"
     CDS             complement(5802..6122)
                     /locus_tag="KR432_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0641804.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         2570 a          603 c          911 g         2044 t
ORIGIN      
        1 ttttaaatga taatgatatt tatttaaatg ctctttatta acaatttata aaacttaatt
       61 gtaaaaaata cttattaata gtaaaataaa gtgaatcaat tatattataa ttgctaaaga
      121 gagtgaggtg tagctatttg aaaaaaaata gattaataat aaagaatata tcaatagcat
      181 ttatagtact attctttttt agtgtattta cattctttta tataggtaat attagtagag
      241 tattagaata tgaaacaaat gacataatga cagttacaat tactggatgg attatacttt
      301 catttttatt tttatgtatt ataatttaca tattatattc aaaagcaaag agtcaaaaaa
      361 ctatagaaaa agtagcatat acggattttg taacaggtta ttccaactgg agaaagtttg
      421 aattagatgt tacaaattta ttaaagaaaa catcacaaaa taataaatat gcaatggtca
      481 tattcgatat tgataaattt aaagcaatta atgatatata tggacataaa aagggcaatt
      541 taatattaaa agatattgca gatactttaa atgaattgac taatatcaat gaaacttttg
      601 ctcgtgtaag tgctgataat tttaatatat tattaacata taacaaaaaa gaggatataa
      661 taaatattat taaaaaaatt atagcaaata atgaacttgt aaacttatct tttggaattt
      721 atgaaatcaa agataaggat ttatctgtaa gtgtatatag tgatagagct tcacttgcaa
      781 aatcatcaat aaaaaataat agtgatgtaa attttgcatt ttttaatgat aaattaagag
      841 agaaattatt acttgaagat aagatagaaa aagaaatgga atatgctctt gaaagtgggc
      901 aattcgtaat gtatttacaa ccaaaatata atattaaatt agataaattt tgtggaagtg
      961 aagccctagt cagatggcaa tatacagaaa aagaagtcat atatccaggt gattttattc
     1021 ctatatttga aaaaaatgga tttattagaa aaattgatat gtacatatta gaacaagctt
     1081 gtaaagaaat ccggtcttta tttgataaag gaatttcacc tttacctata tctgtaaact
     1141 tctcaagagt tgatttttta aaaaaagatt ttattgaaaa catagtaaat atatgtgata
     1201 gatataaaat accatatagt ttaatagaaa ttgaaattac tgaatctagt atgtttggag
     1261 atactgatac tctatttaat gttagtagaa acctacagga tattggtttt atagttgcaa
     1321 tggatgattt tggctcggga tattcttctg taaatatgct taaaaatata ccattaaatg
     1381 taataaaatt agatagagga ttttttgttg atgataaaga tgttgataag agtcaaattg
     1441 ttataaaaag catagtttct ctcattaagc aattaggaat aagggttgtt gctgaaggaa
     1501 ttgaaacaag aagtcaaata gaaatgttaa aaaaagcaaa ttgtgatatt gttcaaggat
     1561 attatttttc aaaaccatta ccaattaaag aatttgaaaa attagtctat aaaatatagt
     1621 cagcgagtct taaatgggag gtggtgatta agtgataaga gcaaaatcgt taaaaaatgt
     1681 actgagtatt gttttaatat ttttatttac aataatttct ttttctgatt ttgcatttaa
     1741 aactaatgtc caggaagaag aaaatagtaa aattaattta agggaaatat ccctacaaag
     1801 ttccaatgct ataaaaataa aattagatag ttctctatcc tttattaaaa gtgtggcaaa
     1861 tatggtttct tattttaata ctatagataa ctcaaaaata gttccattat tagctaacat
     1921 atcaaaagaa tctgactttc aaagaatctc tattgtatct cctgatggaa taagtcatac
     1981 cagcgatggc aaaattatca atgtgtcaaa atatcagtat tttaagaaag ctattaaagg
     2041 agaaagcaat atatctgaag ttgtgaaatc tccaattgat ggaaaagata cttttgttat
     2101 agctactcct atatttaata agaataacaa aataattggt gttttatacg gttcttacta
     2161 caatgataaa ttagctgaat ttatagatat aacaagtttt aatagtgaag ggtacataga
     2221 tatatttgaa tctagtggtc aatttgtttt aaaatcttca catacaaaca aactgattaa
     2281 gtcaagtagt aacatatatg atatatttaa aaaagcttct tttgatgatg gttatagcta
     2341 taaaaaaata tatgaagata caaaaaataa caagagtggc tttacctcat atgaatacaa
     2401 aggagaaaat cgttatgcta cttatacacc cattggtgta aatgactggt atgtaatttc
     2461 tgtgatacca caatatactg taagaagaaa cattgataaa ataaatcaat tagcattttc
     2521 tcttattttc aaagtattct tattgtttat tatgcttata atatatatta tttataaaaa
     2581 caataaatct cagaaagata tactaaaagc taacaaagaa atctcatcta ttacaaatag
     2641 cattcctgga ggagtacaaa agtgtaccct gggtgaaaca tgcaatatta attttttaag
     2701 tgatggattt atagagttgt ttggttatac aagagaagaa ataaaaacat tgtttaataa
     2761 tgatttttat agtttaatac ataaagatga tgttgaaaga ataaaaaaag aaatttctaa
     2821 acaactttac aaaaataaaa ctattgaatt agaatatagg attataacaa aaaatggtaa
     2881 tacagtatgg gttttagata aaggagaact tataacagaa cctgatggaa aagaatattt
     2941 ttgttgtgta actgtagata ttactagaac aaaacaatta gaacaagaga tggaacaaaa
     3001 aaatctagaa cttgaaatga tatcagaaaa tattttaggt ggtataatta taacagaatt
     3061 taatgatgat tttactataa aatacactaa ctctggttat ctttcaatga ttggatatac
     3121 acgtgaacaa ttagaaaatg aacttaaata caaagcttgt aatcttgttt tttatgaaga
     3181 tagaaagaag gctcttgaaa gtatacaaca tcaattaaac acaaaaggaa tagctgtata
     3241 tcaacataga ataagaagaa gagatggtaa tattatctgg gtttctataa aaggaaggct
     3301 tattattgat gatgatggta ttgaaaaagg tatttggatt ctaaatgaca taacagattt
     3361 aaaaaatgca gaacaaaaac ttaaaataaa tgaagaaaga tttagaattg cattaaagca
     3421 aacatcaaat actatatttg agtatgatat aaaaacaaag tcacttttaa atactagtaa
     3481 agagcttgat aaatataata tttataaaat tactaaaaac gttccatatt ctctagtaga
     3541 atcaggagtt attcataaag attttagtga tgattttata aacatgtatg aaaatgtaat
     3601 aaatggagca aaaaaagcaa gttgtgtagt taagatgaaa cttccagatg aaaattatat
     3661 atggaataaa gttaccttga ctactatttt tgatgaagat ggtaatcctg taagagcaat
     3721 tggtatacag gaagatataa gcaaacaaaa ggaagcagag ataagatata atcaagaagg
     3781 taagtatcat tcagctatat tgtcggaagc aatgataacc tatgaaataa atgtatcaaa
     3841 agatactttt atcaaaggaa atgaaagttg gcaaaaacca tttggaatta agcctgacaa
     3901 taattattct gaaatggtaa aaactatatc aaacagtatt attcacaaag acaataaaga
     3961 ggtgttttta aatacatttt tgtgtaaaaa tttattatct gcatataaca aaaatataac
     4021 tgaaataaaa ttagaatatc gaagaatgaa tcaagaaaat aaaatgattt gggtcatttg
     4081 cacaatgcac ttaataaaag attctattac gaatgatata aaagcacttg cttatgttaa
     4141 agatattgac aagcaaaaaa atgaagaaat taaattaaaa ttaaaagctg aaagagatac
     4201 tcttacagga ttatataata aaggtactac agaaaatttg attaatgatt tcttgaactc
     4261 tgacgaagca agcttaggaa tgcatgcatt tttaattgtt gatatagata attttaaatc
     4321 tgtaaatgat aatttaggac atgtatttgg agataaagta ttatttgaag tttctaaaca
     4381 cctaaaacct atatttagaa aagatgatat agtaggtcga attggtggag atgaatttat
     4441 agtattctta aaaaatattc agtcttttga gaataccaaa aagaaagcag aaaaaatatg
     4501 cgatatattt agaaattctt atacggaaaa taaaaagaca tataaaatat ctggaacagt
     4561 tggaatttca tttgtaccag aacatggaac tgtatttgaa gaactttata aaaaagcaga
     4621 catagcactt tattatgcaa agagcaaagg aaaagataag tatgcacttt ataataattg
     4681 tatctaccaa aatgaacttg aaaataatat aagtgataat aatataaaat tagcaaataa
     4741 atgtgagaaa tccaaaccag tacttaaaga aattcttgat tctattgata attatattta
     4801 tatagtgaat ccaaatactt atgaacttct ttttgtcaat aataaggtgt ctcgtttgga
     4861 tttagagata aaattaggag aaacctgtta taaatcacta ttaaaatctg agagaccatg
     4921 taataattgc cctcttaacg gattagaaga aaataagcat tcaaaattca aaatagagac
     4981 aaatacattt ggtaaaaata tcagtatagc aagttggatt aattgcatag atggagaata
     5041 caactgtctt atagagtgta tagattcttc tttatacaaa aataaataaa aaaggactat
     5101 gaagatatgt taaatagtat aactttgata cttggtataa tatttacaat ttactatttt
     5161 tatctcaaaa tagtttttgg aggaatttca tttagtgaga ttttcttagt tgtaggaatt
     5221 gtactaatta tctatcaaat atttaagaaa aaaattaaag aaaagaaggt tttttatagg
     5281 gtgttaaaaa taataatcag cattttttta atagtatttg tagttactga gagtttaatt
     5341 attttttatc ctaaaaatag tttagaatca aaaagtgatt atctcttaat tttaggagct
     5401 tctgtaaaaa agactacacc aagtacaacc ctgaagggta ggttagacac tgcattgaaa
     5461 tacctaaaaa ttaatgataa ttgttatgtg gtagtatctg gtggtaaagg aagtggagaa
     5521 aaaataactg aggcaaaagc tatgaaagat tatcttataa aaaatagtat agataaaaat
     5581 aggataatag aagaggataa atccacgaat acttatgaga attttaaata ttcaaaattg
     5641 aagatagaag aacatagtca aagaaaacta agtgatttaa aagtaaaaat agtaacaaca
     5701 gattttcatg ttctcagaag taaaatatta gcatatagaa atggatataa aaatacgtct
     5761 ttttatgcta gtaaatcaaa actttcattt gtaccaactt actatacaag agaatttttt
     5821 gcattatgga aaacaatcat ttttgataga taataaataa aaagactaga tataaactca
     5881 tatctagtct tttctattcc cttaattcta atccattttc caactttact tgttgtagaa
     5941 atttcaacat tgggatttac aagtaattgc ttataagaag ttttatattt tacgattcca
     6001 aaatataatt ttccttcata atccattata aatccgaatg atcttactct tggtttatta
     6061 tcttcaactg tagcaaaata gaatgttgag ttttctttta aaaaattcat tattttttcc
     6121 atgattaa
//
LOCUS       DAEPOG010000203         6046 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEPOG010000203 DAEPOG010000000
VERSION     DAEPOG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.203"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            157..540
                     /locus_tag="KR432_003295"
     CDS             157..540
                     /locus_tag="KR432_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0641805.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            542..892
                     /locus_tag="KR432_003296"
     CDS             542..892
                     /locus_tag="KR432_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641806.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(986..1444)
                     /locus_tag="KR432_003297"
     CDS             complement(986..1444)
                     /locus_tag="KR432_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641807.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1489..2277)
                     /locus_tag="KR432_003298"
     CDS             complement(1489..2277)
                     /locus_tag="KR432_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0641808.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2416..2853)
                     /locus_tag="KR432_003299"
     CDS             complement(2416..2853)
                     /locus_tag="KR432_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0641809.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3675..4184
                     /locus_tag="KR432_003300"
     CDS             3675..4184
                     /locus_tag="KR432_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641810.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(5095..5895)
                     /locus_tag="KR432_003301"
     CDS             complement(5095..5895)
                     /locus_tag="KR432_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641811.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
BASE COUNT         2176 a          913 c          787 g         2170 t
ORIGIN      
        1 tatatagaaa atccttataa aaatttaaat tgtaatgaat agtaagtaat taaaaagaat
       61 ttttattgat ttttaaaaat ttagttagaa actatatcat tatggtaaag aatgtattat
      121 aatgtttaga aatattataa taaggagcga ttaaacatga atctatcgaa gaaaatagaa
      181 gataaaagaa aagctaaaca gagagctgaa aaagttaaga aagctaaaat tgctacagct
      241 ggtgtggtat taggagctgt tactggtgca gtgagtggag tacttttggc tcctaaatct
      301 ggaaaagaaa caagagagga tataaaagat gcttcacaac aaattgctga aaagataaat
      361 atgaaaactg tagatgtaaa aggcaaagta tctgagaagc tagaggataa aaaaggaaac
      421 tttatagaat caaagaaaaa aataaaaaaa tatatagata acaaaaagag tgttgaaaat
      481 tctagtgagg aagataaaat aatagatata gttgaaccag ttagtgttac tgaggaataa
      541 tatggacaat acaataacga ttaacataag tataatatat gtattattag ctatacttgc
      601 ttgtattgct ttagtttatt taattattgt acttgttaaa ctacataaga ttttaaataa
      661 tgttggaact atgttaaatg aaaatactca aaatataaat acaactttaa ataatcttcc
      721 taatgttgta gaaacttttg gagaagtagg agaaaatatg aaagatgtaa cagaagtagt
      781 tactgaagtt acagcagatt ttcttgttac taaagacagt gtcaaatcaa atatggatgt
      841 tatagtcgat atattgacta taattaaaaa tatttttcta gataaaaagt aataaattta
      901 atataaaata agatataatt taaacgacct taaaataaca tttataaggt cgttttttat
      961 tagcttgatt tgacactatg tgtttttaat aattggtaat attcctcttt atcatttgaa
     1021 catatatatc tatgcttgtt gtttcaatta catccataag aacatcttta cattcaagct
     1081 caaaatcttc cattccttct aaatattcat catcttcatc aaaaaagata taagaattat
     1141 atagccattc ttcaaatttt tcttcaagta tatcccaata cgaactccaa aataatccac
     1201 aacctgatat ataagatgct ctactttgat ttatataaac ctcatttaga agcttttgta
     1261 atctactgct ataaagaaaa tctgctcttc cactgcttga tattaattca tttaaatctt
     1321 ctattgacat taattcaact ttaccacttt tatcataagg agtataattt tcatcaaccc
     1381 atttatgtgg tttgttttct ctgcatactg ttgctatcaa ctctacaact tttgttctta
     1441 tcattttttt atcctaactt atttattttt tataaaataa tcatgatttt ataatttatc
     1501 tatttcttgt aatgctaatt taacagtttc atatctatca gcaccattta ttactacatc
     1561 accttttaca agtttattag cttctccacc tacaacataa acctttttac ctaaaccttg
     1621 cttataacta gacgcctcaa ttattaaaga atctttcaat tgccaatgta aaatctgagc
     1681 agccacttta tcagcatcac ctgtataaac gattacattt tcatacactt tattttcccc
     1741 tcccccttga gaatttgata tagatttgtt tagtatacct tctgctatca atttagcaac
     1801 tttatcttta ttattaacat aataatctgt atctgcttta ctatctacaa aacatacttc
     1861 tattaatatt gctggtgcaa tagtatgatt aagccaataa agacctctaa catcagactt
     1921 tgcaccccta tctttgaata ctgtagctaa cctagtattc actctttctg cataggtttt
     1981 gccattatta gtcttatata ttgtttctgt ccctactgga gttgaagttg tagcatttga
     2041 attaaaatga atctgaacag ccaaatcagt ttcctgttta tttgctatag cacattggtc
     2101 tgctaaatga ttacttgatt catcaactct tcctgtataa acagtagctc cacctatttt
     2161 aagccattta acaattaagt cattcagtat tcttgtttct ttgctttcgt ttatataccc
     2221 aacagctcct gtaccttttc caattaaagt gtgccctggt attattacta ctttcaaact
     2281 aacatctcct ttacaattaa atttaacttc tatttaatat atgcataacc tagaattttg
     2341 tttcttttta tatcttatac ttttattata acccattttg taaaaatata gaatattaag
     2401 taaataatgt taaaattata attttgtata taagacatta cttgattgca ataattttat
     2461 agcatattca tctattctat atccagttct ataatatata tgtttaattc cagcttgaat
     2521 tatagcctta gtacaattta agcaaggaaa gtgagttaca taaatagagc aatctttaac
     2581 tgaaatacct tcttttgcac aataataaag ggcattttgt tcagcgtgaa tggttctaat
     2641 acaatgtcca tctctcattt catgaccaac ttcactacaa tgcttatctc cagaaatact
     2701 accattatat ccagtagaca ctattctgtt ttctgaattg acaattatag ctcctacata
     2761 agctctatca caagttcctc tttcagctac tgtttcgcac agtctcataa aatattcttg
     2821 ccatgagcat ctattttcaa gttttttatt caaatcatat acctccaaaa tttatatcta
     2881 atatataaga tttatacctt aaaaataata ttaaaaacaa atttatacga taaataaaat
     2941 ttattaatac tgtttttagt ctaaattatt tcattttaaa ttaaagttac aaggaatcta
     3001 aaattatata tatctttctt cgtaatatga atgttacaat ataaaaaact gtctcaataa
     3061 aatagagaca gttttacagt caaagtttat aatacaagtt catatttttc tttaaattct
     3121 tttacagctt cgggccatgc cttttgttct gtaatacctt tttctttagc tattttaatt
     3181 gcaatctctc ttatcattgc agcgccttct aatattccca tttttaatct cccttcaaat
     3241 atctttttgt atagaatata aatagtgcta agatttatta catgctgcat atacaatatc
     3301 tatacaacac ataacttgta aattcctttt attttcatac gtctttaacc gacatataat
     3361 tatattcaat aagttttaaa attatgaact caattataat tatttttatt tttttagaat
     3421 ctaaacacta aaaattgtag gtaaatttgt cttatgcctt tataaatatt tctacagttt
     3481 taattactgg acatatctat ttttattata aaaagcgctt aaaaatcgag aatatccgcg
     3541 taaaagctgt atataagcaa aacgattaaa ggaaaaagaa gaaatttact tgaatgttaa
     3601 aatgcgttgc ttgaagcaac gggcagtttt gcctataata tcggtaacaa ctgaaagaaa
     3661 ggtggttagc cctaatgtta agcgaaaaca ttaaaacaat caggaagtca aaaggacttt
     3721 cacaacaaga acttgctatc aaactgaatg tagtgcgaca aacaatatct aaatgggaac
     3781 aaggactgtc ggttcccgat tctgatatgt tgatttccat atcagaggtg cttgaaacgc
     3841 ctgtaagcac tttacttgga gaaactattg ttgagtcgga agttgatagc ttaaaagtga
     3901 tttctgaaaa attagagatt ataaacttgc aacttgcaca aagaaaaaac atgagaagaa
     3961 aaatgtttca ttggttattt attttattat gtgcagtcat agtaatagtt tttgtggtct
     4021 tagggggact aaatagtcct tacttggatt ggaattatac cgaccctgaa accgctgttt
     4081 ttggagtagc ttttcatgct tttgagtggc tatttgtcag attagtaccg attgtcctta
     4141 taggagcaat tattggaatc tttttgacac gtaagaaaat atgaagtaac tctgcttttt
     4201 ataatatagc aagcgttcct taaaagtgtc attctacatg gcaatcgagg acaactatgt
     4261 aaggaagaac ctttttgatt tccaactaag cgaagttatt gaggacgcca gtaggcccaa
     4321 aaccgcatta agtaaagtac aggaagcaag actgctggac ttatactctc atgcgtcaga
     4381 tgcagaaaat gcgaggtctg tagcatgatt tacaacgatt tgtacaacga ctacgagtag
     4441 aaatatatgg agataagtat tatctacgaa atcagaaaag aaatgacttg ataatttact
     4501 catgttataa ttattgatgt gaaattaata aatcgaaatt ccttttttag atttaacaat
     4561 actgagtaag gaaaaagtga atgacttttg caaggaggtg ttaatatcag gcaaataaag
     4621 tcttttgttt ttccagttat atccttaata ttcttagatc aaattagcaa agttcttata
     4681 ggattattct taatggactt tgaaattgat ataattggga aatttttaag attcaatcct
     4741 gttcaaaata caaaattaag tctctgctat ctaacaaacg gagcacaaaa atgaaaatta
     4801 aatattaaat ataaagcaca gttaattttg attaaaaagt tgactgtctt ttctttttta
     4861 taaatttaag catgggtaat aaagaaatga ctgcatttaa gcattatgta tataattgga
     4921 ggcaataaaa agccagcaaa gcccataaaa tagagctgtg ctagcaacat gatgagttat
     4981 aagaagataa tcaaaagctt acgctttatt ttaatttaaa aaaatgatta ctgtaaagaa
     5041 atcatttttt attataatca ctgatacatc aaatattcta aataaaattt ttatttatct
     5101 ggaattgtat attttttcta taatattaac tacagcgtac attgcccaag cacaaatagc
     5161 caatacaaat actcccatca ttactaaatc taatttaaat acttgagaac catatactat
     5221 caagtagcct attccatatc ttgaaactaa aaattctcca actattacac ctacccatgc
     5281 cataccaata ttaattttag ttaaatttat taaatttcct gtgtttgcag gtaatatgag
     5341 tttaaataat atttgagatt tagtagctcc aaagcttttt aacatcttaa ttttttcttc
     5401 atctatattc ataaaataat tataagctga aagtatagta acaacaacag aaatcgttac
     5461 agctgttacg acgattcctt ctattccagc acctacccaa actataatta taggagctaa
     5521 agcagtctta ggaagagcat ttaaaactac caaaaatggg tctaatattt ttgataattt
     5581 ttctgaccac caaagagcaa ttgctaccaa aatacctaat acagtaccta taacaagacc
     5641 aagaacagtt tcatatacag atattccaat atgtttaaat aattgaccac taaatgcata
     5701 ttgaataaat aaattgtaaa tatcacttgg tttgctaaat agaaatgtat ttataacatt
     5761 taagtcagct aaaagttccc agattacaat aaaaccaatt aaaattaata gctggtagaa
     5821 taatacattt cttttttctc tttttacacc tttaacatag tttatatatc cttcagatct
     5881 tttattaagc ctcatgtgta tctagctcct tccataatat gtcgaaatac ttttgaaaat
     5941 caggaacttt acgtgttttg agaggcgttt ttgttccatc catgttaagt tcaatatcgt
     6001 aaatattttt tatggtagct ggtcttttag atagtattgc aacctt
//
LOCUS       DAEPOG010000204         5940 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEPOG010000204 DAEPOG010000000
VERSION     DAEPOG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.204"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            178..1059
                     /locus_tag="KR432_003302"
     CDS             178..1059
                     /locus_tag="KR432_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBH0641812.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1441..2244
                     /locus_tag="KR432_003303"
     CDS             1441..2244
                     /locus_tag="KR432_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641813.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2256..3386
                     /locus_tag="KR432_003304"
     CDS             2256..3386
                     /locus_tag="KR432_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0641814.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            3390..4799
                     /locus_tag="KR432_003305"
     CDS             3390..4799
                     /locus_tag="KR432_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641815.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            5010..5519
                     /locus_tag="KR432_003306"
     CDS             5010..5519
                     /locus_tag="KR432_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0641816.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2409 a          532 c         1065 g         1934 t
ORIGIN      
        1 atagttaata taattttata attggtttag tatagagatt tttaactttt atgttatact
       61 atttataaaa gtaggtataa agattaaaag acataattaa atacgtatga gaggttttaa
      121 atagtattgt taatgaactt gattattgtt tataattaca aattggagga gataaatatg
      181 aaaattactg aaggatatat gccttttaaa ggttttaaaa cgtattaccg tatagtagga
      241 gaaaatacgg aagggaagaa gccattagta ttacttcatg gaggtccagg ttctacacac
      301 aactattttg aggtattgga taagatagct gaaagtggta gacaagtaat aatgtatgac
      361 caaattggtt gtggaaattc ctttgtagaa gggcatcctg agttatttaa tgcagatact
      421 tggatagagg agcttattga actaagaaaa catctaggac ttgatgaaat tcatctgtta
      481 ggtcaatctt ggggaggtat gcaggctatt tggtatgcta ttgaatataa accaaaaggt
      541 attaagtctt acatcctttc atctactctt tcttctgcaa aactttggga gaaagaacaa
      601 aaaagaagaa tatcatatat gagtgaggtt gaccaaaaag cattgcttga tgcagtaaat
      661 actggagatt actctaataa agaatataat gatgcattag aaagatttat ggaaatgtat
      721 tgtgcaggtg aagtgacaga agattctcca gagtgcctga gaagacctaa aaaatcaggt
      781 tctgaagcat atattgtagg atggggacaa aatgaatttt ctcctacagg aactctttct
      841 ggatatgaat ttacagatag attacatgaa ataaaagagc cttgtctagt aactagtgga
      901 gcaatagatt tatgttcgcc atatattgca aagactatgt atgatagaat acctaatagt
      961 aagtgggagt tatttgagta ttcaagacat atgccattcg ttgaagaaaa tgagaaatat
     1021 ataaaagtct taactgagtg gttgaatgcg aacgattaaa aactatataa atttatataa
     1081 agaaatgagt gtctcaaaat gaacttttta gttcattagg cactcttttt gcatgaaatc
     1141 aaatatattt tcattatttg aacgataaaa tgaggcttat atctattttg ggaaagtaat
     1201 tttttattac tagcgttaga ttctaatcta attgtatact tattgaaata atagaaatat
     1261 attggtgttt ataacttaat tttattgttt tttagaaatt tttttatata ttggtataat
     1321 tattctgttt aaatttgatt aaaaagtata ttaattataa taagaaacaa ttatataaat
     1381 aggaatagat tattataatt aatatagata taaaaataat tatagaaaga aaggcacatt
     1441 atggaaaaat tgagagttat tgaaatgata aaaaataatc ccaatattat agctacaata
     1501 gataatccaa ctgatgaaat gaaattgtta gctattaaag aaaatgggct tgtactagaa
     1561 tatataaata atccaaccag agagatgcaa gaactagcaa tagataataa tattagagca
     1621 attaaattta tagataatcc aacagaagat atgatgataa aagcagtaaa tgagggatgg
     1681 agtatactgg attatatcaa aaacccaact gataaagtta ttgaaatggc tataaatcaa
     1741 gcaggatggg caattaaata tgctaaaaat ccaagtgaag aattacaact gttagcagta
     1801 agaaaaaatt atgattcgat aaagtttata aaacaacctt atgaaagtgt acaagaggag
     1861 gcagtaagaa taagttatga tgccttgaga tatatagatt cacctagtta taatgcagaa
     1921 cttatagcta taaaaaataa tgaagcagct attagcttta taactgattt agataaaaat
     1981 aaaatgttgg aatttttaaa agtgaatatt ttagtgatta agtacattct gaaaaaagta
     2041 tctaaagatg aattagagag tgtattaaaa gaagtattat ccaaagaaga tgttgaggaa
     2101 aagtatatta gagatttttt gaattgtagt gttatagata gaaatagcga aagtatacca
     2161 atagataaaa ttatgtttat ttataaatat ggaagtaaga cagcaaaaaa aatagctgta
     2221 gatgagaaat taaagattat gtaggagaga gaaaaatgaa ttttatagac acattaaaaa
     2281 gcgttgactt aatattatcc actggagaag tacctttaat agtaggtgaa agtggaatag
     2341 gaaaaacagc tttagcaaat aagcttgcca aagaaaatga ttggagttta attgttattg
     2401 atggaaactt acttaaagag ggtgaaatag gtggtcttcc aactatagaa tcttatgtag
     2461 gagttaattc taatggatat aagactgaaa agaaaacaac agtgtatgct gttcataaca
     2521 aattaagaga aattgatgaa gagatatcta aaagtaagac agttctttta tttatagatg
     2581 agataaatcg ttgtgagcat acagtacagc aagaacttat gaatttaatt ttaaatagag
     2641 aaattaatgg atataaactg catgatgatg taaagatttt agcagcaatg aatccatcaa
     2701 gtaaatatgg ttcagatttt gattatcaag tggtagatat ggatgcagcc caagaaaata
     2761 ggtttgtttg gttaaatatg gagagtgacc atacacaatg gataaaatgg gcaatagatg
     2821 agggtattga aagaaaagtt atcgagttta tttccacttt tccagaatat ctgcataaaa
     2881 taaatgaaga tgatgtaaga gctactccta gaagttatga gagagtatct aaaatttata
     2941 aagtttataa agaaaaaaat aattctattc ctagagcagt atttttaaat gttgtaaagg
     3001 gtaatgtagg taaagttatt gcagaagaat ttataagttt tattgaatca aactctgaac
     3061 cattaatatc ctatgaagat gtttttttaa gagaatctat tgatgagccc attgtagaaa
     3121 gagtaaaaaa tgagagtcat acaagacttt atctatcagc tatgaacatt ctaaaagact
     3181 tagaattaaa tattaagaat gataaatatg aatcaaacca ctatattaat agatttattg
     3241 aatttttaaa aatgtatcct gtagatttaa tgataggtat tatgaaggat attagaaaca
     3301 gttatattga agtatataaa aaagccatag aaaatgaagg ttttgtaaaa tcatattttg
     3361 aatcttatag cttaataagg ggataatgta tggaaaatta ttttgataaa caagcaaaag
     3421 agctttataa taaagcaagt gaaattataa atactcattc tatgctaaag gcaaatcgtg
     3481 ctaatgagaa atttgatatg gatataccac aagattttaa gtatgagttt tttagtcttg
     3541 tagataaagt gaatttaagt cttatggaag aagaagataa tttttatgga tattttttgt
     3601 ttcagatgtc aagagaaata agatttgata taagtagtcc tacaggcgtg aattttaagg
     3661 gagctaaata tgttatatat tttaatccaa taatcttttt aacgcttgac ataaagcaaa
     3721 tggagactac aattaaacat gaaattcatc atatattatc tatgcatttg atgagagcaa
     3781 aggagttaaa aggaaagtat agtacattgg caattaatat ggcaatggat atagtagtaa
     3841 ataaatttct aaacaattta ccaccatatg caactacctt agagtgggta aatttaaaat
     3901 attccttaaa acttgaacca tatgaacctt ttgaatatta tgtagaaaag attcagactg
     3961 aattagattt gatggaagtg gatgaagatg gtgaggaaga tgatagtgat aaatatggag
     4021 atgtagaaat tgattatagc cccgaaagaa ctcatgatat atgggatgaa tccaatgaga
     4081 tagataaaaa tacccttaga gaatttactg aaaaatttat taacagttct caaaaaggta
     4141 aagttcctgc ttatttagaa agtatgatat catcacttaa aaatagtaag ggtgaattgc
     4201 catggaattt atatcttaat agaataatgg gaacagttga aaataataaa aagaagacta
     4261 taacaagaag aaatagaaga caaccaaatc gtttagattt gaggggagaa cttagaagtc
     4321 ataaggcaga aatagcagtt gcacttgatg taagtggaag tattagtgat gaagaattta
     4381 aacaagctat taaagaagtt cttagtatag tgaaaaacta taatcatgaa attacaatta
     4441 tagaatgtga taatgaaatt agacgtgtat ataaagtgag aactgaaaaa gatataaaag
     4501 atagaattag gataggtgga ggtactagat tttctccagt ctttgagtat gctaataata
     4561 aaaagattaa tttgttagtc tactttactg atggtaaagg tgaaaataaa ctacaagtaa
     4621 tacctagagg gtataaaatt ttatgggtta tttctggaag aggagataaa atttcattaa
     4681 aagaacctta tggagcagtt aaaaaactta ctaaggttaa aacaaatgat gaaatattag
     4741 aaataaatga tgtaagaaat gatgggtact caatgaatag ccaagcacca attttatagg
     4801 tattcacagg agataagctg tattaattta ttttgatatg cttgttataa aaatctaaaa
     4861 aatacattaa tcaatggtta atgtattttt taattttaat aatataagtt ttacttacat
     4921 ttaagtctta aaatgtgtta aaatataata tatagcagat agttttggca agatggattt
     4981 agttgtcaaa actatttttt ttattaagga tggtgatgtt tttgtttaag attgatgatt
     5041 atataatgta tggtatgaca ggagtctgca aagtcatgga tataacaaat gaacgattta
     5101 caaatggaat aaaaaaagag tattatgtat tgagtcctat ttattctaat aatacagtaa
     5161 taaaaattcc tgtagataat gaaaaagtac caatgagaaa gttactttct aaaattaatg
     5221 tgctttcatt aataaatgat attcctaata tggatacatc atggatagat aatgaaaaat
     5281 taagaagtga gcaatttaaa aaaatactca gaggtggtaa atgtgaagag ttaataaaat
     5341 tagtaagaag tatagataat aacagagagt atgtaaaatc tattggtaaa aaaacacatc
     5401 aagcagatga taatataatg aaagaagcag aaaggttatt aagtgaagaa tttgcaacta
     5461 ttttagatat ttctcctaat gaagttagtt catatatttc aagtcatata cctcaataaa
     5521 gtgttttagt atcctttatt taatctattt taagtattat ttatgtatgc tggttttggt
     5581 aaaataaaat atataattaa attatataaa aaattaagaa tgattgtatt gaagaattac
     5641 agaatagtgc ggagagccca ctcctttagg tgtgagatgg atatcactta aagatttaaa
     5701 ttgttattgt agatgttttt aatataaagc aagattaata agtgtattgt aagctttaag
     5761 tgaaattgat actataatga gttacttaga ttttaaaata tataatatta taaaaaatcc
     5821 ttttaggttt tgaatgaata agatggatac tatgttgaat agaatccatc ttatttaagt
     5881 tttatttatg ttgtttattt gtaagcatta tttttgtaca tgaaaacaca gtgtaaaaat
//
LOCUS       DAEPOG010000205         5815 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEPOG010000205 DAEPOG010000000
VERSION     DAEPOG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.205"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            496..1701
                     /locus_tag="KR432_003307"
     CDS             496..1701
                     /locus_tag="KR432_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0641817.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1772..1993)
                     /locus_tag="KR432_003308"
     CDS             complement(1772..1993)
                     /locus_tag="KR432_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641818.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KR432_003309"
     CDS             complement(2134..2733)
                     /gene="recR"
                     /locus_tag="KR432_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH0641819.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2746..3096)
                     /locus_tag="KR432_003310"
     CDS             complement(2746..3096)
                     /locus_tag="KR432_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH0641820.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KR432_003311"
     CDS             complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="KR432_003311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH0641821.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4869..5133)
                     /gene="ffs"
                     /locus_tag="KR432_003312"
     ncRNA           complement(4869..5133)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KR432_003312"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5171..5262)
                     /locus_tag="KR432_003313"
     tRNA            complement(5171..5262)
                     /locus_tag="KR432_003313"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5226..5228),aa:Ser,seq:gga)
     gene            complement(5263..5718)
                     /locus_tag="KR432_003314"
     CDS             complement(5263..5718)
                     /locus_tag="KR432_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBH0641822.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1847 a         1020 c          653 g         2295 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1741 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1801 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1861 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1921 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1981 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     2041 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     2101 aaactacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     2161 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     2221 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtcgc
     2281 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     2341 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2401 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2461 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2521 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2581 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2641 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2701 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2761 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2821 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2881 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2941 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     3001 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     3061 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     3121 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     3181 tatattttat ttctaatatc tcttcactca ctatgttctt taatatctcc tcaccaatgt
     3241 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     3301 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3361 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3421 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3481 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3541 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3601 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3661 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3721 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3781 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3841 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3901 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3961 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     4021 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     4081 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     4141 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     4201 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     4261 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     4321 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4381 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4441 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4501 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcac
     4561 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4621 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4681 gtcctatatt atcactatat atttgatttt ttaaagtctt aataatatga tcttgaccaa
     4741 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4801 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4861 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4921 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4981 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     5041 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     5101 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     5161 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     5221 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     5281 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     5341 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5401 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5461 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5521 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5581 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5641 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5701 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5761 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatag
//
LOCUS       DAEPOG010000206         5811 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEPOG010000206 DAEPOG010000000
VERSION     DAEPOG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.206"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            83..2314
                     /gene="pflB"
                     /locus_tag="KR432_003315"
     CDS             83..2314
                     /gene="pflB"
                     /locus_tag="KR432_003315"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH0641823.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2474..3208
                     /gene="pflA"
                     /locus_tag="KR432_003316"
     CDS             2474..3208
                     /gene="pflA"
                     /locus_tag="KR432_003316"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBH0641824.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3330..5000)
                     /locus_tag="KR432_003317"
     CDS             complement(3330..5000)
                     /locus_tag="KR432_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBH0641825.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2061 a          830 c          834 g         2086 t
ORIGIN      
        1 aaaatatata aaataaatta agtatataaa aaataatttt aatagtatta tttatcattt
       61 aaatctaagg aggaaatact taatgaatgc atggcaagga tttaaaacag gaagatggac
      121 taaagaaata aatgttagag agtttataca attaaactat tctccatatg agggaaatga
      181 ctctttctta gctggtgcca ctgaaaatac taaaaaatta tgggacgaag caatggtcct
      241 atttaaaaaa gaaagagaaa atggtggtac tttagatgtt gatacaaaaa ctgtttctgg
      301 aatagctgca tatgctccag gatatttaga taaagaatta gaaacaatag ttgggttaca
      361 aacggatgcc cctttaaaaa gagctgtcat gccatatggt ggtataaaga tggttgaaaa
      421 ttcatgcgaa gcttttggat atgaattaga cccagaaata aaagatatat ttactaaata
      481 cagaaaaaca cacaaccaag gagtttttga tgtttatact ccagaaatga gagctgctag
      541 aaaatctggt ataataacag gtcttccaga tgcttatggt agaggtagaa taattggtga
      601 ctacagaaga gttgctctat acggtgttga tgctttaata gaagataaaa atgagcaaaa
      661 gaaatcatta gaagtttctt gcatggatga ggaagttatc agattaagag aagaaataac
      721 tgagcaaata tcagctttaa atgaacttaa aaaaatggct gaatcttatg gatttgatat
      781 atcaaaacca gcaactaact caaaagaagc tgtacaatgg ttatactttg gttatttagg
      841 tgctgttaaa gaccaaaatg gtgctgcaat gtctttaggt agaacatcta ctttcttaga
      901 tatatacttt gaaagagatt taaaagctgg aatcgtaaca gaagaagaat tacaagagta
      961 tatggaccat tttgttatga aattaagaat ggttaagttc ctaagaactc ctgattacaa
     1021 taacctattc tctggagacc caacttgggt aactgagtgt ataggaggta tgggaataga
     1081 tggtagaaca ttagttacta aaaactcatt tagaatgtta aatactctat atacattagg
     1141 accttctcca gagccaaact taacagttct atggtcaact aaattacctc aaggattcaa
     1201 agatttctgt tctaaagtat ctatagatac aagttcagta cagtatgaaa atgatgattt
     1261 aatgagagca tactggggag atgactatgg tatagcttgt tgtgtatctg caatgagaat
     1321 tggtaaacaa atgcagttct ttggagctag agttaactta gctaaaactt tattatacgc
     1381 tataaatggt ggtgtagatg agaaatctgg tgttcaagtt ggaccaagat ttgaacctat
     1441 aacttctgag tacttagatt atgatgaagt tatgagtaaa tttgaaccat tcactgattg
     1501 gttagcaaca ttatatgtaa atactttaaa tgtaatacat tatatgcatg ataaatattc
     1561 ttatgaagca ttagaaatgg cattacatga tagagatata tttagaacta tggcttgtgg
     1621 tatggctggg ttatcagttt gtgcagactc attatctgct ataaaacatg ctaaggttaa
     1681 aactattaga aatgaacaag gaatagctgt tgattttgaa atagaaggag attatccaaa
     1741 atacggaaac aatgatgata gagttgatag tatagctgta gagttagttg aaagctttat
     1801 gaataaaata agaaaaaata aaacttatag aaattcttat ccaactcaat ctatacttac
     1861 aataacttca aatgttgttt atggtaagaa aactggtaat acaccagatg gtagaagagc
     1921 tggtgctcca tttgctcctg gtgctaaccc aatgcatggt agagatacta atggtgcttt
     1981 agcttcatta tcttcagttg ctaaattacc atatgaacat gctcaagatg gtatatctaa
     2041 tactttctct atagtacctg ctgctttagg aaaagatatg actgaaagaa taaacaatct
     2101 ttctgcaatg atggatggat acttctctca aaatgctcat cacttaaatg taaatgtatt
     2161 tgatagagct actttagaag atgctatgga acatccagaa gaatatccac aattaactat
     2221 aagagtatct ggatatgcag ttaacttcat aaaattaact aaagagcaac aattagatgt
     2281 tataaacaga acattccacg gtaaaatggc ttagtcttta aatttttata ctttaactaa
     2341 gattactact gtgtaaaaaa taaaaataaa aggctatctt atatttgaat tatttctggt
     2401 ataagatggc cttttttaga caaatattat aactattgat aaggttatta aaatatattg
     2461 tgaggtgaac tctatgatta agggaaagat acattctata gaaacgtttg gtactgtaga
     2521 tggaccagga ataagatata tattattttt tcaggggtgt cctcttagat gcaaatactg
     2581 tcataacagg gatacatggg atattaaatc aggaaaagaa tacacagttg atgaaataat
     2641 aacggatgct cttaagtata cttcttttat gaagttttct ggtggaggta taactgcctc
     2701 tggtggtgaa tccactcttc aaccagaatt tttaagtgaa ttatttaaaa aagcaaaaga
     2761 aaatgatata cacacttgct tggatacatc tggattcgta gatatagaaa ctatagaccc
     2821 agttttagat aatactgatt tggttttact tgatttaaaa catatggttg aggaaaaatc
     2881 tattgatttg actggtgttg gtatggataa ggcattaaaa cttgctagac acttagaatc
     2941 aagaaatatt cctgtatgga taagacatgt tttagttcct ggcattactg atgatataga
     3001 taatttagaa aaactaggtc aatttgtagc tacattgaaa aatgtagaaa gatttgaatt
     3061 gctaccatac cattctatgg gaatacacaa atgggaaagt ttaggtatcg attatgaact
     3121 aaaagatgtt cctgatgcca ctaaggaaga tgtccaaaaa gctagtgaaa taatctctaa
     3181 atttggagtg aaagtatata atagttagtg tagtatatta atcctttata gaagacataa
     3241 caaaataggg taaagtgtaa atttttaata aacttcaaaa cttccacttt gccctatttt
     3301 tatatactat atatctcatt ttatgtacat cactttgata gttgaaattt ataaaattca
     3361 gaggcactta caggtcttcc aaataaaaaa ccctgaataa tatctacacc taagcttttt
     3421 actatatcat actcttcata agactctatt ccctcaacac aaacttttaa atttataata
     3481 tgagctaatt taataatata ttcaagaaaa gtatattcat aactattata tgtaatattc
     3541 ttaacaaaac ttctttcaat ttttattata ttaacagaca gttctttaag gtaatttaaa
     3601 gatgaatacc ctgtaccaaa gtcatctatt gcaatatata ctcctatacc ctttaaacta
     3661 ataaatttat catttagtaa atttatatct ggtatccaac aattctctgt taattccaaa
     3721 attaaaaatt caggtcttaa ttgatactct actaaacatt ctactataaa atctctaaaa
     3781 aaatcttctt ttaactgaat atacgataca tttacagata ttttaaagtc tggatttatt
     3841 ttatgccatt ctttacactg ttttacagct tcttttataa tccacttccc aactggtata
     3901 attaaattac tctgttctag tattggtata aattcaacag gagatacttc tccataagta
     3961 ctcgaatgcc atcttaaaag tacctctgcc cctattacct cttttgtaac agcatttacc
     4021 tggggttgaa aaaacagctc aaagtcatta aaattatttt caacgcactc tcttagtttt
     4081 tgttgcattg aaattacctt tagtttattt tcatagagct cttgtgaaaa aaacttaatt
     4141 ctatttttac cactaatttt tgctatatcc aatgcaatat cagcatgttt aaataaatct
     4201 aaataattat ctccatcttc tggatacata gcaactcctg cagtcactgt gcaatagtac
     4261 ttatttgatt caatttcatg ctgagtattt gtatacatct gtatcttttc atatattttc
     4321 tctatagttt ctttagtaca catagggtaa aaaaatgcaa attcatctcc atctaaccta
     4381 tacaatctta catcttttgg aagatattct aatacttcag tagcaattgc acgcaatact
     4441 ttgtcaccat aagaatgtcc atatttttca tttatatttt taaaattatc aatatccatg
     4501 ataaacatag caccattttt ataaattttc tcttttataa gataattcat tctatgttca
     4561 aattgttctc tattccacgt tcctgtgata gaatcaaatt tatttttttc accaatattc
     4621 tttattctcc cgactagaaa tatagttttt gggtcatccg acacataaac tttacctcta
     4681 caagaaatcc atactatttc ttcatattta ttaatcaacc tatattctaa attgtgctca
     4741 cctttttttc catgtagtaa ttcttgtata tcatctttcc atatttgaac atcatccgga
     4801 taaacaattt tactccaaca attgactaaa tcagattcta tctctttact taagttaaat
     4861 tcatcaaaaa ttgctgaaga aattttaaat ttattcctat ttatatccca aaaaaatata
     4921 taatcttctg tactttgaga taaaagttca aagaaaattt ctatatggtc taaacttaaa
     4981 gagttcgatt cttctctcat cttttccccc tacaatatta ctattagtgt agtttaatca
     5041 acaaaatatt cttatttaga ttatactatt ttactaattt ttttatctca tcaaacacat
     5101 ttccccaact ttagttatct tatcaaatct cttactctta actaagtttt tttgagattc
     5161 ataaagtaat ttttctacaa tcattactgg tgatttattt tcacctgcta aaacaccatg
     5221 ttaaatcatt tattaaatca ttttctctta aaagtccatt ttttattata taaagagttc
     5281 tcagttcttt attctataaa gcttttctat tactcttgaa tttgttttat tttttctcag
     5341 cctattctct ttggatttgg tattgctttt ctatatattt tggcaaagaa gtgcctcttc
     5401 ctataatata agattttgat atttcttcat catctataaa ttttttaata gggattatat
     5461 cattttttat atttatttat ataaatattt tttgctttta attttttatc tcttattatg
     5521 tacttacatt taatttattc ttttcagtta ataaaatagg tacattttta gtacatgaaa
     5581 atagagttgc ttacagggta tctaggattg atgtactatt tactagtatt acattttcag
     5641 ccgattcaac ccctcttttg cttacataat ttaagtatat ttttctggat ttttaaattt
     5701 atgtacttta aaatggatat ccttattttc attgatttta taagaattaa taaaaaatac
     5761 gattacacta cagaattaga atgtcaatgt ttataaatat aaaataatat t
//
LOCUS       DAEPOG010000207         5719 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEPOG010000207 DAEPOG010000000
VERSION     DAEPOG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.207"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            240..1067
                     /locus_tag="KR432_003318"
     CDS             240..1067
                     /locus_tag="KR432_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0641826.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2084..2548
                     /locus_tag="KR432_003319"
     CDS             2084..2548
                     /locus_tag="KR432_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641827.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(2815..3459)
                     /locus_tag="KR432_003320"
     CDS             complement(2815..3459)
                     /locus_tag="KR432_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641828.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3389..4678)
                     /locus_tag="KR432_003321"
     CDS             complement(3389..4678)
                     /locus_tag="KR432_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641829.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4675..5547)
                     /locus_tag="KR432_003322"
     CDS             complement(4675..5547)
                     /locus_tag="KR432_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641830.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2075 a          783 c          704 g         2157 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagttggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 aaatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggagaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggggttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattattaa attttagaaa atttattaaa aaatataagt
     1621 taaattaata tttttaaaga ttctttttta taactactag aatactaatt tagtagttat
     1681 tgtatctcta aagtttaagc atggatagat atacatttga taatttgcct ataatttacc
     1741 tactacttgg agtattggag caaatacaat atattaataa taagttccta aaaccaataa
     1801 attaactaat acacataaag atattgatgt tgattataca actataagaa acagatttaa
     1861 cagaatttaa tcaagctaag aaattacata tatcgtgatg tttttgaatt agaaggtaat
     1921 tcaaaattag tttatagtct tctgtgaatc caataaaaag tacagggtac aagaattgtt
     1981 agtaaaattt ttaaagattt tatggataga tataaatagt tttacttatt ttgtaaaatt
     2041 taaaatacca ttaattacct taaatatttt aataggagga catatgaatt taaataaagt
     2101 taaatttact gaagaacatg ctattgaagt tactaattgg aaatatgaag gtaaatattc
     2161 aatatacaac ctacctccat gggaggaaat taaaaagaag aatttttcac tatcaaaaga
     2221 agataaaaga aaaaatttta taagttttat aaatgacaat aaagaattga taggttttat
     2281 aaacttattg gatgaaggaa gttatgtatt ctttggtatt ggtataaaac cagattattg
     2341 tggaatggga attggcaaag aaataattag cctaggatta caagaatgta gaaataaata
     2401 tagtactaaa ccaattgtat taaatgtaag aacctggaac atgagagctg taaaatgtta
     2461 taagtctcaa ggatttaaaa tagttgagac tcaggtacag aaaacgcatt taggggatgg
     2521 agaatttttt gttatgaggt atcaatagtt tataacacta gtcatattgc taatatgaca
     2581 attaaaatga aaaaatacat gtgtgtatgt catagcaata agtgtctttg aaatataaat
     2641 ttgagagcct ttgataaaat tcggtataca tatatatttt atactacaga tatataaatc
     2701 tatctacata cataaagaga ttgtaagtag gtttgtgtat ataaaaataa atttatattt
     2761 cataaatgta ctttttattt gtattacctt tattttatct aagctctcat tttattacct
     2821 aatcttcctt tttattggtt ttattaagag agatgcaatt atcaacacaa cacttccaag
     2881 tatcagccaa tatttctcat caaaactacc ttgactcata gacattataa aaaattttcc
     2941 aacatacttg cctttagtca tcatatttaa aattaaatat cctatagata tcatgtaacc
     3001 tatgattgta ttattaaata aatttgagaa aaacattcct aaacttccta taaacatagc
     3061 tgatgcgaaa cttccaataa tatactcaac tactggaaaa ttacatccaa gatatattaa
     3121 aacatttaca aaacctatga ttaataccac tatacctata attgacatca gtatcctaat
     3181 taattgtaca ataacttgag ataatgattt tgaagatact atctcatcaa tttcttttga
     3241 ttgctctagc gaaaatacag gtaccaacat tactatccct attattgcaa cataattttc
     3301 taaacatata gctgttgaaa ttctgtctaa attagctact ccttgtatta gaggaatcat
     3361 acatagcaat ataattgaac atattaaatt agttaaagtg cttaatttga agttttcttt
     3421 tattagaact aaacagtttt gaaaactttg cccaaacatc aagattcccc cttctttttt
     3481 gctcatataa gaatatagct aacaaaacaa gcaccaatgc tcccaagctt atcacaattc
     3541 tattaatcat aatttgattg aagttttgta taataatatc cccatttaat acactattat
     3601 gtcttatcat tagccccatt gttgaataat cacctttgag tcctgtagaa tttatactgt
     3661 aaagccacca aattaattgc acaataattg ctactggtgt atctgttaat atagtaatac
     3721 tcattcctac agctgtaact atcataagag ttggtagtat ccaccataat gtatatttta
     3781 taaatgctaa gttatcaata gatatacctt tatctttagc aaagaaaatc aaaggaagta
     3841 aatctttcat acctaaaatt aatacaggca atatcattat aaagacaagt gcaaaatatc
     3901 ttgataaaac tattttgcat gaagatactt gttttgaata aataatctct ctcattcttg
     3961 aacgcttatc tttaagtgac ataaaaactg ccacaaatac tggaaatata cttaaaacta
     4021 tacctatata atcgcaaaaa attcttgcat acccattact aactttatct ttttgtattg
     4081 tatagttgta attcttcata gcttcatcat aggtaactgg aactactcca aatctttgta
     4141 acttgtcaaa agcatatgca gaccctcctc ctaaaatgtc gtctactctt tgcattattt
     4201 cttcaaatct ttcaagagat actttagaat aatctatttt tatactttca tcagttttcc
     4261 aaattaaatt tatttcgcta atacttttac cagttatttc ttctatatat ttttctatgg
     4321 tcttcgtttt attttggtct aattttatat acttaactac ccctgttgga tacgttgtat
     4381 atgtgttttt ttgatattct ctaattaggt ttatagttgc atttggaatg accttttctg
     4441 ttttcataga ttctttttga ccatatggat attgattgtg tacagagtaa tattcgtcaa
     4501 tattctctac tttttgtgga gcaactaatg gatttccatc attaggaata tttttactta
     4561 catcatttga aactgaactt ccaagttgag aaatatagaa aaaaactaaa actacaataa
     4621 aaatataata taccatactc tttaatatat ttttacattc ttttataaat agattcatat
     4681 attatctcct cccattataa gcatatagcc atcttcaata gttggattac aatttactgc
     4741 tgtctcaaaa ggtttatcat ctgatataat cctaagtata gcctttccac catgagtcaa
     4801 cattcctata actataaatc tactttttat attttcaatg tcttttttat ctacttcaat
     4861 actatatact ttcccttcag ctagttttgc taatgcttct gttgagccat tgtatatgac
     4921 ttttccacta tccaatatag ctacgttttc acatgtagct tctatatctc caacaatatg
     4981 tgtagataaa ataacgattc tatcatttgc aatttcgcat agtaaatttc taaatcttat
     5041 acgttcttct gggtcaagac ctgctgtcgg ctcatccact attaaaactc tgggattatg
     5101 aattaatgct tgtgctatcc caagccttct attcatacct ccagataaag attttacttt
     5161 cacattttga taattagtaa ggtttacttt gcttaataac tttgctataa tttttcttct
     5221 ttttcttagt ggtatatccg ataaaactgc taaataatcc attgctttat atactgtcat
     5281 gttaggatac attgaaaaat cttgtggcaa ataaccaacc atatttctaa tttccttagt
     5341 attctcaata ggaactccac acatatgtat tgaaccttct gtttttgata ataaagtggc
     5401 taaaattctc ataaatgtgg tttttcctgc tccatttctt cctaaaagac caaacatacc
     5461 actgtcgatt tcaagattta tattatcaag agcttgtttt ttcccaaatc ttttactaag
     5521 attggatact gctattttca tttccatata taaatacacc cccagaaata attttataga
     5581 attttatttt tataaatttt tactgtattt tcataataaa ttctaaaact taagatatta
     5641 tttataaatt tctaaatttt ttctaaagat tttaattttt ctgtttttaa ttaaattaaa
     5701 gtatctttgt ttctgaaat
//
LOCUS       DAEPOG010000208         5613 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEPOG010000208 DAEPOG010000000
VERSION     DAEPOG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.208"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            30..881
                     /locus_tag="KR432_003323"
     CDS             30..881
                     /locus_tag="KR432_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBH0641831.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1014..2627)
                     /gene="ggt"
                     /locus_tag="KR432_003324"
     CDS             complement(1014..2627)
                     /gene="ggt"
                     /locus_tag="KR432_003324"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH0641832.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2852..4789)
                     /locus_tag="KR432_003325"
     CDS             complement(2852..4789)
                     /locus_tag="KR432_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641833.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4776..5543)
                     /locus_tag="KR432_003326"
     CDS             complement(4776..5543)
                     /locus_tag="KR432_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641834.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1896 a          906 c          630 g         2181 t
ORIGIN      
        1 agataatata aacataggag gtacattctt tgaaaaacat tatttcagca aaagaactta
       61 tatcaaaact agaaaaaaat gataatctag ttgtaataga ttgcagattt gatcttatca
      121 atagaactta tggtattgat tcttataaaa aaggacatat caaaggcagt tttatcttag
      181 atatagacaa ggatttatca tctcctacaa aagaacatgg tggtaaaaat cctttacaag
      241 accctttaat actaaaagaa aaattagaaa aaatgggtgt tgataatgac actacgattg
      301 taacttatga tgatggagat ttaaatggag cttgcagatt atttttccaa ttaaaacatt
      361 tggggcttaa aaatgtctat gtactagatg ggggaataac atctttcgta aaggaaggtg
      421 gagaattgga agagaaagtg aatataccaa gttgtacagg taaggaaatt agaccaaata
      481 taaacaatga tttagttgtt ccaatggaat atgttaagtc taaattatac aaaaaagata
      541 ctgtaataat agattgccgt gctaatgaga gataccaggg tttagttgaa cctgcttact
      601 caaaagctgg tcatatacca agtgcaaaaa attatttttg taaggattta ataaaaagtg
      661 actttgaaaa tggctcactt aaagatatag aatttttaaa aaaattcttt aaagatttaa
      721 ataattatga tgaagtaatt ttatcttgtg gttcagggat atctgcttgt gttaacagtc
      781 ttgctcttag agaactggat atacctcaca agatttatat tggtagcttt agtgactgga
      841 ttagttatga tgacaatgaa atcaaaactg gtcaagaata aggctcataa tctatataaa
      901 atcagcatat gatagcttaa attttgtaaa ggcgtcagtc attgtagact ttcgccttta
      961 ctaattattt catatactct ttaaatttat tatatatatt ttagttgata atattaataa
     1021 actgcaatat gaccatcaca tcttgattcc gtcccacaaa tataactttc ctgttctgtt
     1081 ttaaatatta tttgacctcg tcccatgtcg acagtatcat gaattacttt tatttcatga
     1141 cccttttcaa ttaatgaatc tattatatgc tttggcatct ctggttcaac ttctatttcc
     1201 tttcctttta tccactgcca tcttggagca tctagcgcct cttgtggatt taatccaaaa
     1261 tcaatcatat ttgttaacac ttgaatatgt ccttgtggtt gcataaaagc tcccattaca
     1321 ccaaatggac ctattgcttt atcttcttta cctaaaaatc caggtattat agtatgataa
     1381 ggttttttaa atggttttac tacattgtgg tgtttagggt ctaaattaaa attatttcct
     1441 ctactatgta aagcaatccc agttcttggt atcacaattc ctgaaccaaa atttatgtaa
     1501 ttgctctgta tatatgaaac catatttcca tccttatctg cagtacaaag gtatactgta
     1561 ccaccacaat atggctctcc tgcagttgga tacaaagcct ctttattgtc aattaataaa
     1621 cttctttttt tggcatactc ttggcttagt aattcctgaa tctttacctt catatgctct
     1681 atatcagtta catatgtttt agaatctgca aaagctaatt ttaaactctc tataatttta
     1741 tgtatgtctt ctacattttc tatatttcct tctaattcca attcttttaa tatgttaagt
     1801 gccattaaaa ctgttattcc atgaccattt ggtgggattt caaatatttt atatccctta
     1861 tattctgtac ttataggttc tacccatgtt ggataaaaac actctaagtc actctttctt
     1921 atagccccat taaatttttt agaatattca tctattttat cagcaagtct tcctctataa
     1981 aagctttcag cctgagtatt tgctatctct tctaatgtac ttgcctgttc ttcacatata
     2041 aatatatctc cagcctctgg agctttacca tctttagaaa atgtatcaaa ccatggtttg
     2101 aactcttctc caataagttc tttttcatag agttcatatg attttttcca taccttagct
     2161 acattaggag atactacgta tccctctctt gcatagttta tcgctggaga taagcattcc
     2221 attaagctaa gtttaccata ttttttattt aattctgacc aagctgcggg tattccaggt
     2281 acagttactg ctccaaaccc atattttggc atttctttca tattattata attttttaat
     2341 tccatatttt ctggagcaaa gccactagaa tttaaaccat acaatcgttt ttcttcctct
     2401 atccaaacaa gggcatatgc atctcctcca atcccattgc ttgtaggttc tactactgct
     2461 aatgtggcgg ctgttgcaac tgctgcatca acagcatttc cacccttttt tagtatttca
     2521 agtccagctt gagatgctaa tggtgttgat gttgcaactg ctccattttt agcatacacg
     2581 acatttctta ttgatgagta tttatgttgg taggcattaa acttcataaa tttcagctcc
     2641 tcgtcatttt ttatttgata ttctgcttag atttattata taacatcaag taatgtaact
     2701 gatacaaaat gatataaaat atagactttt tcctaacttt aattttatct ttaaataaat
     2761 atcttaaact caattctagt attttctatc tgcacttaaa taagttattt tattcaaaac
     2821 atatatctaa ttcttttgac ttaaaaataa tttattcttt tatcatgttt agagcatatt
     2881 ttaaagttaa aaaataatat ataaaatatc ctataaaata aatgactaga gttatgataa
     2941 ttggtacaag taatgacctt ggtataaact tttgtgctat agacatagca aataaattat
     3001 gaactgtacc tacaactaat ggcattaaaa atataatttt taactgtttt gatattattt
     3061 tctcaatatc tttattattt gctccaagtt ttattaacat aatatatctt tccttatcat
     3121 catatatatt tgataattgt ttaaataaga ttatactacc tgtacataaa agaaatacta
     3181 cagacaaaaa tatgcctata aacagcatta gaccacttaa ttttatcaag ttattataac
     3241 tttcaatact tgatgtaaaa ttaaaaggat aagtaaattt catattttca cttactatat
     3301 ttttaaattt taaagtcaaa tccagttctt ttttttcatt ttttatatca attaatctta
     3361 ttaatctaga atttccatgt aattttaact gattaaatac gtcatctttt actacaatta
     3421 aatcaaacgt agcttgtgaa tttatttctg gctccaagat gcttttttgt accttaaaca
     3481 gatttatatc atactcattt gtatctttaa tatacaagtt tattttttca atctcaaagt
     3541 ctttaaaaaa catttttttg tatgaatcag aaacatagta cacagattcc tttgaagtta
     3601 attcttcata attgttattc tgatattgtc ttaatttttt aaaatcactt tctctaataa
     3661 tatctacaga agttctatct tctatataac tatttccatt tttataatac atttcaatat
     3721 ttatatcttt gtctataagt tctattgttt tatcaaagat tatcttatta tcttttttat
     3781 atttatataa taatttgtca atttcgttat ttacataaga atttcctaaa tttatggtat
     3841 aactgtattt ataattttca cttatgttcc tatctatatc gtaataaagt gatatggtaa
     3901 accctagcat agttacactc gtagctatta atattgctat tacagccaat acactactat
     3961 tgctctttaa tttaaatcca agttcagaaa atgcaattaa attctgtccc ttataataaa
     4021 aactttttct cttcttaaca gcatcaataa tcatatacat agttgatgaa aaaaataaaa
     4081 aagttccagg tattattgta actaatataa aaactgaaag cataatattt tcagcaataa
     4141 aataactaat tgacattaaa taagaaatag ctattaataa aacaccaagt acacatttta
     4201 actttataaa ttttttaaac ttatactttt ctggatattt attaaataac tctataattc
     4261 tcttatttct gataactata gtatttctta ttccaataac tataaatatt attaaaaata
     4321 ccgctagtgt cttaactaag gcctttatac ttaagttcat atttactact atcatttcat
     4381 tcattaattt tacaagcatc atagtcataa gctttgaaaa taacagtcct aaaaatatac
     4441 ctattagtat tgcaactata cccatcaaaa aagtttcata taaaaatatt tttcctatct
     4501 gatttttcct cattccaagt aaactataag ttcctacttc tcgcctacgt ctattgaaaa
     4561 aaaatgagtt tgaataatat ataaataaaa atacaaatac cactattaca attgaacttg
     4621 tattaatact catcctagtt ttttctccaa gtgaactcaa cgcttcatta tactctatag
     4681 atttaaaact aaaaaataca aatacactaa atacaattga aataaagtac attaaataat
     4741 taaaaaaact attctttaca ttttgcattg caattctaaa taaagtcatt tttattaact
     4801 cctccaatta aggaaactgt atttactatt ttattataga actctctgtt actttctccc
     4861 tttacaattt ctgtgaatat tattccatcc tttataaaga gtattctatt gcaaaaactc
     4921 gcacttatag catcatgagt taccatcaat attgttttat tgctgtcttt gtttaatttt
     4981 aatagacaat tcaacaaatc cctagcagat ttagaatcta atgctcctgt aggctcatct
     5041 gcaagtacta atgatggttt tgttgctata gctctacata cagctcctct ttgcttttgt
     5101 ccaccagaaa cttcgccagg atatttatct aatatagatt ctatatttaa ttcttttgaa
     5161 atctctttta ttctattttc tattactgat actctttcat tttttaaaga aagaggcaac
     5221 attatatttt cttttattgt aagtgtatcc aaaagattag aatcttgaaa tacaaagcct
     5281 agattatcac gtctaaaatc tgccaattca ttttgcttca ttttaatagt attctttcca
     5341 tttatataaa tatctcctga tgaaggtaaa tccattgtag aaataatatt taaaagtgtt
     5401 gatttaccag caccggaaga ccccataata ccaacaaatt ctcctttata aaccttgaaa
     5461 gttattcctt ttagagcttc atgttttgaa ccttttcttc catactcctt ttttatattt
     5521 tcaacagata atatttcttc catagttaat aatcaccttt catcacaatt agttatttat
     5581 caattctcac aaatatatta tatatattaa aat
//
LOCUS       DAEPOG010000209         5600 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEPOG010000209 DAEPOG010000000
VERSION     DAEPOG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.209"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            857..2266
                     /locus_tag="KR432_003327"
     CDS             857..2266
                     /locus_tag="KR432_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0641835.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            2282..3484
                     /locus_tag="KR432_003328"
     CDS             2282..3484
                     /locus_tag="KR432_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641836.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3685..4668
                     /locus_tag="KR432_003329"
     CDS             3685..4668
                     /locus_tag="KR432_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641837.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(5118..5561)
                     /locus_tag="KR432_003330"
     CDS             complement(5118..5561)
                     /locus_tag="KR432_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641838.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
BASE COUNT         2264 a          643 c          770 g         1923 t
ORIGIN      
        1 tggaatgata aaaataagaa ttaagtgtaa tacaatgtaa ataaaatgtg ttaattttaa
       61 gtatattttg taaagtttat aaaatctgaa aagctcaaga tatattttca ctataatgga
      121 taaaatatca aaatattgaa ataaacaaaa aataatgata taataagaaa aaataaagaa
      181 aaaaagtaac ccttagttgt aaaagggtta tttttttgtg ataaaatatc cataaaaata
      241 ttcataccaa agtaaattaa gatgtatttc atttctcaaa aatatcctaa tataaaaaat
      301 aaagaaatat ttataaattt aattattaga catagataat atctaaaaaa tataccaatt
      361 tcaacaaata gtaacttttt tacaacaaat ggaaactatg gaagcaataa ttaaaataaa
      421 atttataatc aatcataatt atttagtata atgattcgat attattttaa agtatttgtc
      481 atttaaaaca aacttgcgat aaaagcatat ctgaaagtat tctgattcta atacttaata
      541 aagagttctt tttacagtta tagatataaa caacatctat cttaatagac tgtatagagg
      601 cacaatcaaa ggtaaacaca tatagttaag taatatgtcc ttgatttttc tttattataa
      661 tagctttatt aattcatttt aatattacat cattttaaaa aaattatatg aaaagtcaaa
      721 atttacatat agattaatta aacttgtaaa aacacatata aattcataca attaagtact
      781 taaaacttga aaatacttac tttgctatat attttagata aagatataat tgatttggtt
      841 aagaaaggag tctaatatga tattcagtag tttaatattt ttatttttat tctttccagt
      901 cacactcatt tgctattact tggcaaatcc taagtataaa aatgttatgt tacttctagc
      961 aagtattgca ttctacgcat ggggagaacc aaaatatatt tttttgatga tactatcaat
     1021 aatagtaaac tatatatttg gtattaaagt ttctggcagt aaaaataaaa aaatgtgggt
     1081 tgtgctttct gttatttaca atatatctat tcttgctgta tttaagtata gcaatttttt
     1141 tatagataat attaattttt tattcaatac agatataact atacctgagg ttaaattacc
     1201 tttaggtatc tcatttttca cattccaaat aatgagctat gtaatagatg tatatagaaa
     1261 tgatgctaaa gttcaaagaa aattagctaa tttagcatta tacataagtt tattccctca
     1321 gcttgtagca ggtcctattg ttagatatca gactgttgca gatagcattg agtatagaga
     1381 acacaattgg gacaaattta ctcaaggtgt aaacagattt gttgttggtc ttggaaaaaa
     1441 agtaattcta gcaaatcaat taggtgtaat cgcagatggt gtatttaata agtcagctgg
     1501 agatttatca agacttgagg tatggctagg tatagtttgc tatacacttc aaatattcta
     1561 tgactttagt ggctatagtg atatggccat tggtttaggt aagatgtttg gatttgagtt
     1621 tttagaaaat ttcaactatc catatatctc tcaaacagta tctgagtttt ggagaagatg
     1681 gcatatatct ttaggtactt ggtttaaaga ctatgtgtac ttcccattag gtggaagtag
     1741 agtctctaag gcaaaactat atagaaatct atttgtagta tggtttttaa ccgggatgtg
     1801 gcatggagct agttggactt ttattatatg gggtctatat tatggaatat taatatcagt
     1861 agaaaaagca ttcttagaaa aatttctata taagatgcca agaattgtaa gacacatata
     1921 tctacttctt atagtcatga ttggatgggt attttttaga gttgatgatt taccagcagc
     1981 tattaaattt ataggtatta tgtttggtgc tggaaattat ccggttatca gtgatgcatt
     2041 caatgtattt ataattgaat attggtatat aattgttggg gcaatccttc tatcaactcc
     2101 tattgtaaaa gttgtaaaat cattattaat taagaaaaat aaaaatacat taaatagcac
     2161 attatatcaa gttactaatg tggttttcat tgtttcttgt atgtttattg taataataat
     2221 gttaagtagc tcaacctata atccattctt atattttaga ttctaattgg gaggtataaa
     2281 catgagtaaa aaaaaaccaa ggattgttgc catttcattt ttaattatag tatttggatt
     2341 tttcatacta aacttcacac ttccaaagaa aaaattaagt ataactgaaa atagaaatct
     2401 tgcaaaattt ccgactgtag aaaatttaaa aaaatgtgac tttatagcca agtttgaaga
     2461 atattttaat gaccattttg catttagaga tgaaatgata tcaataaata caaggactca
     2521 ggctaattta aataaaacta aggtaggtaa ctattatcta ggaaaagata actggatatt
     2581 aggtatgttt ccagaaattt tagaccaaaa acaattaagt cgatacgaaa aatccattaa
     2641 ttatttatct aatctaagta aagatatggg aaaaaatgtg tattttacaa tgatgccaca
     2701 taaaactaat atgttaaaac atttgtatcc taaatatgtt gataatatgg ataatataga
     2761 cataaattta aagaatttta aaaatagatt aaatccagat ttaattaaat atatagatat
     2821 ggatgagtat tttttaagta actattcaga taaagaaaga gaaaatttat attttaaaac
     2881 tgaccatcat tggactggat atggagcatt tgaaggcttt aaaatgatgg catcaaatat
     2941 ggacttaggt ctatctaagc aagatttaga caagcatttt agtagctata aaaagtctat
     3001 aattactgat aagaaattta taggaagtta taatcaaaat atagatatgc taattaaaga
     3061 aaatgaatat gtagtttata tgacaaaaga taaccaagat tatagctttg atataaatgg
     3121 agaagaaaaa gaagaaaaag aagaaaaaga agtttatgca actgaaagag ataataaaga
     3181 gtgggattat ggtggtgcat acattagagg agctaaaact aatatattaa ctataaaaaa
     3241 ccctaaagca ttagtagata aaaaaatact aatatttaga gattcatatc aggctcctac
     3301 aacactttta tttgctgata tgtttaaaga agtagagata gcagacccta gaaatataaa
     3361 ctcaatatat aaaacataca aagagataat cgaggaaagt aatgcagata ttgtaatgtt
     3421 tatgtataat agttctgggt ttgattcaat gatagattca atgatagata aaggaataaa
     3481 gtagtaaata aaaagcctct attaaattca aaatttaata gaggcttttt attgtttatt
     3541 ttttattgct ttggtaaaaa taaactaatc aaaataaaag taaggagatg tagcatattg
     3601 aacaaaagac atagtctacc tatagtatca gtgatattat tgatggtctt aattatcttt
     3661 aataataacc aaaattatat tacaatgaaa gaagttttta atgtccatat tatgcagagt
     3721 caaaataaaa agattaaaca aaagtattat ctaactaaag atggatggct attaggcaac
     3781 tttgctaaat tattatctga ttatcatata aaatcttact ccaaagccat taataacttg
     3841 agtaagttaa atagaaaaaa agatttttac tttgtttcac ttcctcataa gacaaatatg
     3901 cttaagcact tgtatccaag tggtatagaa ggtaaaaata atatagatat taacaaagaa
     3961 aacttagaaa agcagttaac agacataaat ttcttagata tagatgatta cttttataga
     4021 agttttaccg aaaaagagag agaaaaattc tttttaaaaa cagaccatca cggaaattca
     4081 ttaggagcat ttgaagaata taagtttatt cttgataaat tagaccttgg cttatctaaa
     4141 tcagaaatag atagtcattt aaaaagatac agtactcgat atgtaaatga caaggtcttt
     4201 ataggtagtt ataataaaaa gatgggatat ccacttaaga aaaaagagtc tataagctat
     4261 gttgaaataa gtgatgcaaa ttacaaatat tttattagta atggtactaa aaatactgag
     4321 attaatgaaa atatcataaa agcaaaatta aaagataaaa gagaatggga ctatggtgga
     4381 gcttatatga gaggttgtga ttgtcacata cttaagatta gaaattcaag ctctttaact
     4441 aataaaaagg cccttatatt tagagattcg taccaagcac ctatgacttg gatgttggct
     4501 gatactttta aggaactcga aatactagat cctagatata taaaaaatat agatatgacc
     4561 tatgatgaga taattaaaaa cagtaatgcg aatgtagttt tatttatgta taatagcttt
     4621 ggatttgctg gaatgataca agaaatgatt gacaagggaa tagcataaca atttgaatca
     4681 gctccttttt atacaaaaaa tagttctcaa taattcctaa tctatatgat aatttagtac
     4741 atctctttaa caataaaaat aaaatcacaa aaattcttat accatcactt atctttatga
     4801 taaattggtt gagaaaaaat tttataattt atatgatatg caatcttaat taattcttta
     4861 tctactgcaa cctttaataa aagaaaccat ccaagactat taatatgaat ttatataaaa
     4921 attactttca aataaagtac tgcagtattt ttatttaaag tatctataat ctttaaccac
     4981 aaaaagccac aaaatacaat gtatacttat ggctttttat aaatttatat aattgtatat
     5041 aaagatatat tctaaaactg tcttaagaat ctaaagttta aaaatccaat atttttattg
     5101 tgaatttaaa ctttttatta caacttttta tctaatttat tgtatacaac aacactacaa
     5161 ataaattgaa ataaaataag caatccaaga acaactaaaa cccacttgct tattaccatt
     5221 acagaaaata cactcaatat agaaacaata aatataagaa ttacaagaac ataggacatg
     5281 atttgattag tgatacgtcc agctttatca cgtaacataa ctcttctttc atcattcatc
     5341 tctatccttt ggttttttat cttttcttca taatctactg ctctttcagg cttactccaa
     5401 tacatatatt gaaaaatcat acatatacct ggacctaaag cagctccaga taatccccaa
     5461 ataaaagcct caaatgaaaa ctcagtaaac gtagccaata aaacaagtaa aattccacaa
     5521 ataaaatata ctattccaag atataacata ttctttttca tttttactcc tcctcataaa
     5581 taaatatttc ttcaatactc
//
LOCUS       DAEPOG010000210         5558 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEPOG010000210 DAEPOG010000000
VERSION     DAEPOG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.210"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..519
                     /locus_tag="KR432_003331"
     CDS             <1..519
                     /locus_tag="KR432_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBH0641839.1"
                     /translation="MNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEG
                     LLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDGYKTMPSQL
                     NILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRE
                     LTDEEVKLIEER"
     gene            546..1907
                     /gene="rlmD"
                     /locus_tag="KR432_003332"
     CDS             546..1907
                     /gene="rlmD"
                     /locus_tag="KR432_003332"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0641840.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2430..3464
                     /locus_tag="KR432_003333"
     CDS             2430..3464
                     /locus_tag="KR432_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBH0641841.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3551..3754
                     /locus_tag="KR432_003334"
     CDS             3551..3754
                     /locus_tag="KR432_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBH0641842.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            3925..5085
                     /locus_tag="KR432_003335"
     CDS             3925..5085
                     /locus_tag="KR432_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBH0641843.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2204 a          547 c         1071 g         1736 t
ORIGIN      
        1 atgaataaac ctgatggata tatatcagca actacagata aatatgaccc aacagtatta
       61 gatttaattg atttatcata cttagctttt gaaccatttc ctgttggaag gttagataaa
      121 gacacagagg gattattagt tttaactaat gatggaaagc tttctcatag agttttatct
      181 cctaagaagc atgtgcctaa gacttattat gcaaaaattg atggagtagt tactgaagaa
      241 gatgtagaag cttttttaga aggtgtagtg cttgatgacg gatataaaac tatgccatca
      301 cagttaaata tattaaaaag tgatgatgaa tcagaaatag agcttacaat acatgaaggt
      361 aagtttcacc aagtaaaaag aatgtttgaa agcgtaggca aaaaagtagt atacttaaaa
      421 agattgtcta tgggcaattt aaaattggat gaaagtttag agttaggtga gtatagagag
      481 ttaacagatg aagaagtaaa attgattgaa gaaagataat aaagatacta tagtaaattg
      541 gaggaatgtc tgtgaagaaa aaagatgtta tagaatttga agtagataaa atggaatttg
      601 gtggtacatc tttaagtcaa gttggagata gagttatcca tatgaagggt ggaataagtg
      661 gtcaaaaggt gagagctgga gttaaaaaag ttagaagtaa gaaagctgaa gttaagatga
      721 tagaattatt agagaactca ccacttgaaa ctgaagaaac ttgcaagcac tttagagagt
      781 gtggaggatg tacactattg tctgtgccat atgaaaaaca attagaaata aaagaaaaac
      841 aagttatgga tttattttta aagcaagatt tatttggatt tcagttttta gggatagaag
      901 gaagtccaga aaacaaatat tatagaaata agatggaata tacttttgga gatgaagtaa
      961 aaaatggacc tttgacatta ggtcttcata aaaaaggaaa gcatatagat atacaaactg
     1021 ttgaggagtg tatgttggtt gatgaagact tctcaaagat attagtttct agtgtagagt
     1081 ttttcaatga aaaaaagctt ccttattata gaacaatgaa tcacaaagga tatttgagac
     1141 atcttgtagt tagaaaaggg attcatacta atgagattat ggttaatata gtaacttctt
     1201 ctcaagaaga ttttgatatg aatgagttta aagatatgct tttagggatt gatttaaagg
     1261 ctgagttagt aggtgtactt catactatta atgatggttt agcagatgca gtccaatgtg
     1321 atgagttaag agtattgtat ggaagagatt atatacaaga agaaatttta ggtcttaagt
     1381 ttaagatttc accattttcg tttttccaaa ccaatactaa gggagctgag gttctttata
     1441 gtattgctag ggattttatt ggagattaca atgataaggt tgtatttgac ttgtatagtg
     1501 gtactgggtc tattggacag gttatggctg gtgctgctaa gaaagtgtat ggtattgaga
     1561 tagttgagga agctgttgtt gctgctaatg agaatgctaa gttgaatgga cttactaatt
     1621 gtgagtttat tgctggggat gtggctaagg ttgttaagga tttaaaggac aagcctgatt
     1681 tgattattgt tgaccctcct agaccaggca ttcataagga tgctattagg gatatttgtg
     1741 gatttggtgc taaggagatt gtttatattt cttgtaatcc taagtctttg gttgttgatt
     1801 tggttgattt taaggggtat ggttatgaga ttaagatggt taagtgtatg gatatgttcc
     1861 caaatacgcc acattgtgag actgtagtta aacttattag agaataacag ggtttcagac
     1921 ttttatattg tgtcttaaaa gtagatttgc cacctatttg ccaccataat tatttttggg
     1981 tggcaaattt attctacaat gatttcaaaa atatttacca tctggtttct catgttatta
     2041 gttacatgta aatatgtatc catagttgtt gaagttttag catgtccgag tatatgctgg
     2101 atatctttta tattagtacc attttctaat aacatagtat cagggttttt aaaataaagt
     2161 gtgtattctt ctagagtagt gataattaaa atatcaaata tcactataca aaggaaagtg
     2221 cacccttttt atgtttagca aaaacgaaat attaaaaaaa cttattgaag aatatgatgt
     2281 caaaacaact acagatattc aagatatgct aaaagattta tttacaaata caattcatca
     2341 aatatttcca ttgtcgaatt ttaacggttt aaacataata tattgactga tataataaaa
     2401 tcatagaata ttttaggggg aaatagatta tgtttaagaa aaagttaata gctgttgcaa
     2461 tgagtataac tatgattagt gtagggagct tttcgtatgc tcattcagga agaactgatt
     2521 catcaggagg tcatcgtgat aataaaaata aatctggatt aggaagttat cactatcatt
     2581 gtggtggtta tccagcccat ttacataata atggtggttg tccttatact ggaggaggaa
     2641 gcagttctgg aacaacaact tcggttaata atgaagaaaa gcaaaagagg tctgttggag
     2701 aaaaaggata taatcaaggg tatgaagatg ggtataaagg aaattatagt tcatcaaatt
     2761 atagtggaga ttatagtgat acatatgaat caaagtactc agaaggatat gaaaaaggta
     2821 aggctaaatt agaagaggaa gaaaaggtgg ctaaagagac tggatataat ttagggctta
     2881 caggtgctaa atctaataat acatatgaaa aagaagtttt aaaaaatgct tatgataccg
     2941 gatattcaac tggatacaat gaatataaga ctaaaaagat agaagaatat aaagctaaag
     3001 ggatagagga tagcaataaa gataaagaaa aaatgacatt tgaagaaaac atagattctg
     3061 aatttataga tgcttataat aatgcttatg acgaaataca agaacaatta aaaaatgatt
     3121 acactactca aggatttgag agtgctatca aaggagaaag atttgacaca tcaactatag
     3181 gtaatgttaa atatgctaat tggtttaaag aaggatatga taatggtaag gttaagttac
     3241 ctaaagcaaa agaaagtgta tataatcaag gttacaatga agaagatttt agtataccat
     3301 atgaaatgaa gtctattgaa actaagttaa aaggtgtata tgatgaaggt ttagaaaaaa
     3361 gagaagaaga gaaaagtaga aatgctactt atggggttgg agctggaaca gcagctgtag
     3421 ttgctggagt agtatataaa aataaaaaac gtaaaaagat atagatgcta gatataccaa
     3481 tgatgacaag tgtataacaa atataatttt aagtataaat atttaaaata tagaagggaa
     3541 ggttatatct atgggattta actttaggaa aagcataaat ctaggaggag gactcaaatt
     3601 aaatttaggt aagaagagtg taggtataag tgctggtgtt aaaggtgcta gagtgtcagt
     3661 taattcaaaa ggaagaaaat cagctacatt gagtatacct ggaactggaa tatcatatac
     3721 aaaaactagt acaaagaaac gtaaacctaa ataaatattt gatatactaa gaaatagact
     3781 gttacaaata gtctattttt tagtatatag atttaaaaag tgacaaaatt ttgaaatata
     3841 aactaatttc ataaaggaaa atattctttt atgtagaatt ataatattta agtaatatta
     3901 taaaatttat aggaggattt cattatgaaa ttaagcaaaa cactattggc aatagggatg
     3961 tctgtttcat tattgattgc tagttgtcca acatcaaacg ctcttagttc tattaattca
     4021 ataaaaggta caaataagta tgaaacagca ggtttaatag cagataaaca aaattatgat
     4081 attgcaatac ttattaattc tgaaaatgga ttatctgatg gattgtcagc aagtggattg
     4141 tcaggagcta aaaatgcccc aatattgtta actaagaaag atacaatccc ttcagaaaca
     4201 ttaaaaagat tagaaaaggt aaaagaagta tatctaattg gaggaaaaaa ttcgatagga
     4261 gcatcagttc agaaaatatt aagtgataaa aaaataagca taaatagaat aagtggaact
     4321 gatagaatag atacaagtca taaagttgca aaagaaatag gaagagtaag tacggttaaa
     4381 aaagtaatat taactaatgc ttttaaaggc gaaccagatg ctatgagtgt tgcttctata
     4441 gcagttagag ataaatctcc aatagtatta actgatggaa atactacaag ttttgctaca
     4501 gatggattag aaacttatgc aattggtgga aaagtcagta tgagtgataa gttaataaat
     4561 gaaactaaag caacaagaat aggtggagta gatagatacg atacaaataa acaaataata
     4621 aataaatttt ataatggctc aaaagaattt tacatagcaa gtgggactga tttagtttat
     4681 tctttagttg gttctacaat tgcacaaaaa acacctatag tgttagttgg aaatggaagt
     4741 aataagagta tattaaaaaa tgcaagtaaa ataactgcta ttggaaatat tgatgatgtt
     4801 attataaaac aatgtttaaa tataactaat aatgttgggg attcaaatac aggtattgta
     4861 aaagatacta attcaaataa tacaaacaaa ccaagtaatg agcctagctc taataatcct
     4921 ggattaactg ataaaagtac agtatatgta actcctaatg gaaaatcata tcatctaaca
     4981 aaaaattgtt ctacactaaa aagaagtaaa acaataaata aagtaacatt aaaacaagct
     5041 aagtcacaag gtaaaataga cccttgtaat gtttgtgtaa aataatgtat tagccaaata
     5101 tatattaaaa aagacaatta attaagattg tcttttttat tgaagaaata taaatttata
     5161 ttatgtatta ttaaaaagaa aattattaat tataaaaaat aataataaag acgataagta
     5221 ttaaaatttt atgtttccaa tatttatgat ataattaata tggcaaggaa taaatatttg
     5281 aaaaagctat gagtggtgtt tccatggaag tttcttcact ttcttatgag aggaggtgaa
     5341 aactatggaa aatctaatga tgagtattat aactggtgtt atagccagtt acatttacga
     5401 taaattaaag agccactctg accaatgaac tatacccact gttttataca gttaaaacat
     5461 gtaaaagtgg ttataaagta ctagtgtcaa tcattagtac tttttttatt atattcatat
     5521 aattaatagc tatattttaa agtttactat ctaaaaaa
//
LOCUS       DAEPOG010000211         5554 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEPOG010000211 DAEPOG010000000
VERSION     DAEPOG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.211"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(533..1084)
                     /locus_tag="KR432_003336"
     CDS             complement(533..1084)
                     /locus_tag="KR432_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641844.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1275..2237
                     /locus_tag="KR432_003337"
     CDS             1275..2237
                     /locus_tag="KR432_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBH0641845.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2532..4376
                     /locus_tag="KR432_003338"
     CDS             2532..4376
                     /locus_tag="KR432_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0641846.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4698..5282
                     /locus_tag="KR432_003339"
     CDS             4698..5282
                     /locus_tag="KR432_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0641847.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2160 a          557 c          874 g         1963 t
ORIGIN      
        1 atttagatat aggagtgtta tgataaaagt aagattatgt gttgtttata ttaaatataa
       61 agtgcatata gtgataaaac aaaagtttca tataaatgaa gaaaaaatat aaaataattt
      121 atttaactac ttaaaaataa actaaaaggg ataataaaat agcttttagg ttatggtaat
      181 aaaggtattt aaaaagagtg cataagcact ccaattgtga gaaatacata cacaaatcat
      241 tattacaaca atgaaaaatg tacatataag agacgttttg tatttgtttt aagtgataat
      301 attttaatac taactaaata attatcacaa taataaaaaa gaaaaaggag tattttgtac
      361 tccatttgtc aaaaatataa aacttttata taagcttatt ataacataat tgataaaagt
      421 gtaaagctat ataatgaatt tataaagaaa aataaaataa aaaagataaa tcctaatcaa
      481 aggatttatc tttttgtata gaaaataatt attctattat taaatggcat ggctaaactg
      541 gataataatt tattattact gctttcaatg ttttttctcc ttgattatgg tatgtatgaa
      601 ttgattcagc tgaaaaacaa atagagtcat tttctctaag ttgataaata gagtcattta
      661 cttctaattt taattgacct tctataatca taatatattc ctgtgatttt tcagaatgac
      721 caacagatgt ataactatga ccttcttcta attccatttg aaataattcg aagtttctag
      781 ttggtgtatt gggataataa caataaagac gataatcttt attttctgaa acttgtacat
      841 ctatatcagt ttttgataca ataaaagttt cattttgagg ttgttccagt attgcagtat
      901 atggaacatt tagtccactt gcaattttcc aaattgtgtt tactgttggg tttgagtctc
      961 ccttttcaat ttgcgaaagc attactttac ttacgccaga taattcagca agttgaccta
     1021 gacttaagtt acgttcatta cgtaatctat ttaaattttt agcaataaca gtattaatac
     1081 tcatagtaac tccttaaaat gtaaattatt gacaaatata atttacgaat gtataattta
     1141 tttaacatta tttataattt acaaatgtat aatataatga acgttaaata cactgtacat
     1201 ccgtaatata aattatactg cataaagagt tgtttttcaa cggatgatag ttgagagatt
     1261 aagggggaat tgtaatgcgt gtacttaata atgttacaga ggctataggc aatactcata
     1321 tgttacattt atccaaaata gcagatgaat ttggagttca aggtaatatt tatgcaaaga
     1381 tggaatactt aaatccggga tttagtaaaa aggaccgtgt agcattacaa atgattaccg
     1441 aagcagaaaa tgcaggttta ttagttccag gtcaagcagt tgtggaatta acaagtggta
     1501 atactggaac aggactagca atagcttgtg catgtaaagg atataaattt atagcgtgta
     1561 tgtcaaaagg aaattctatg gaaagagcaa gaatgatgaa agctttaggt gctgaagtag
     1621 ttcttgtaga ccaaatgcct aattcagttc acggacaggt atctggtgaa gatttggcac
     1681 ttgtagaaat tgaggctcaa aaaatagcaa aagaaagaaa tgcatttaga gcagaccaat
     1741 ttaatttaga agcttgtaat catgcacatg aaaagtatac agcagaagaa atgtgggaac
     1801 aattggatgg aaatattgat gtatttgtag attttcttgg aagtggtagt acttttgcag
     1861 gatgttcaaa aacacttaaa aaacataatt cttctatcaa atgttatgtt gttgaacctg
     1921 aaacagcagc aatttattca ggtaaagaaa taactgacca agggcataaa atacaaggtg
     1981 gaggatattc tatggatttg ccattagtag acaagaaact tattgatggt tatattcaag
     2041 ttacagacga ggaggcaact gatgtagcaa gaaaattagc taaattagaa ggtgtatttg
     2101 caggattttc ttcaggggca aatgtgtgtg cagcaattaa gttgttaaag agcagtgaaa
     2161 aaggaaaaaa tatagtttta actcttaatg atagtggttt aaaatattta agtactgatt
     2221 tatatgaaga attgtaataa tagcacaata gttatgcaat gttttttaaa ttaagtatat
     2281 agatttaaat tataaaaagt atctcaatgt gatttttgaa agttataaaa gagatacttt
     2341 tttattttgt aaatatggag tatcctactt ataagtatta agctatatag atattgatga
     2401 ttagctatgc aataaaaaag agtatgtgtt tttgcattta tacatcataa cttggaataa
     2461 tacatattaa tgttctaaat gtatttaaaa catatgataa aattagaaca aacagataca
     2521 aggagataaa catgcaaaag agaattttaa gaaattttat atttacgggt aaaaatattt
     2581 taagtaaact tgtttttata ataaaatata attttagtac attaatttta tttgaattat
     2641 ttcataaagg attagcctta ttttttatat ggccatctat tagatatata tttgatattt
     2701 taataaaaag tttacatata gtctacctaa atatggataa ttttataaaa gttttgacca
     2761 atcccatgtc aatagttctg ataatactat cagttataat actagctttt tatgcatttt
     2821 ttgaatttac ctctgtaatt atttgtttta ataaatcgat aaaatatgaa aaaatagggt
     2881 tatttgaact ctttaaaata tcatttaaga actctataaa attgttatat cctaaaaata
     2941 tactattatt tatatttgtg atattaataa ttccattagt aaatacagtt ttaatttcag
     3001 ggtttatagg aaaaatacaa ataccagagt acatattaga ttatataaag tctgatataa
     3061 ttttaaatat aatctatatg atttttcttt taataatcta tatttcggtt attagatgga
     3121 tttttagcat tcatgagata actttaaata cagaaaattt taaaagagca agaaaagaaa
     3181 gtgttaatct tataaagaga aagattataa gaacaattat ttattcatta atattatctt
     3241 tagtagtagc tattattgga tatgcgattt atcatagtgg aattatattt ataggcatat
     3301 ggactaaata ttgtactgat atggaatttt tgaagaatgt atttataaat agatgtattg
     3361 catttgaaga atattttaga ttgattttat caatattcat ttttatattg gacattggat
     3421 ttatctcagc aacttattat caatataaag gagtaaatat ttctaaaaat aatattaaaa
     3481 aagagaaaaa atctatatca aaaattgttt ttaatttagt attaatatta ttgatttctt
     3541 atatagagtc agttgcattt tcttcacata taaatggcat gtttaatacg tcattttcat
     3601 tttatacaac agcaattgct catagagcag gagctgaaat ggctccagag aatactttgg
     3661 cagcaataga agaagcttca aatgctaaag ctgattatgc agaaatagat gttcaagaaa
     3721 ctaaagatgg agaacttatt gtacttcatg attcaaactt taagagaact acaggagtag
     3781 ataaaaatgt ttgggatgta aattataatg aagtaaagac atatgatgcg ggaagctttt
     3841 attattatgg aaaaggttat tatgatgaaa agtttgttgg ggaaaagata ccaacactca
     3901 aagaaatgat aaaatattct aaaggtaagg tgaaattatt aatagaaata aaacttaatg
     3961 gacatgaaaa gggagacata gtaaaaaaag ttgttcagtt aattaaagaa aataattttg
     4021 aaaatcaatg tatagttgca tctatggata aaactatatt acaaaaagta aaaaaactgg
     4081 atgagaattt actgacatgc tattttatgg caattgcata tggtgatttt tcaaagttaa
     4141 attatgttga tatatatgga attgaatcaa cttttgttaa taaaacagta gtagaaaaga
     4201 ttcataaaat gaacaaaaaa atcttcgttt ggactgttaa tagtgacagt ttaattgaga
     4261 aaatgcttga cctaaatgta gatagtatta ttacagataa tccatacctt atagagagtg
     4321 caatttattg gaagaaaaat ggatttatta gtcaagtgtc agattattta ttttaaatat
     4381 tctataattg tattatccag taaaagagat attattaaaa tcaataatat ctctttttaa
     4441 cataaataag gttgttaaat taaggttatt cttataaaaa ttgtgcttat cagctataaa
     4501 caaggaataa aagtgaaaaa attgctctga gattattaca gttaaaagag ttttaagatg
     4561 acagagagat agattgtcaa ctgtatattt aataaatctt gttatttatt aaattttata
     4621 taagtaaaat atataaatta ataaatctta tgctagaata tatatgaaaa acaatataaa
     4681 tttggaggat taaaaacatg acagagcgag aaaaaatgtt ggcaggagaa ctttacgatt
     4741 gtggtgatat tgagttgtta actcaatggc ataaagcaaa agacctagta agagattata
     4801 accaaacaga ttcctcggat ttggaaaaaa aagaaaaaat attaaatgaa ttacttggtg
     4861 gtaaggataa aaacttatgg attactgcac cgtattatgt agattatgga aataatattt
     4921 attttggaaa taattgtgaa gtcaatatga attgtacgtt tttagatgat aataaaatta
     4981 tcattgggga taatgcacta atcgcaccaa acgtacaaat atatactgca tttcatccaa
     5041 ctaatgctgg tgatagattt ggtaatgtca aagaagatgg ttcatttgaa ttttgtaaaa
     5101 ctcaaacagc tccagttact attggagata atgtttggat tggcgggggt gctattatca
     5161 tgccaggtgt tactattgga gataatgtag ttataggtgc aggaagtatt gttacaaaag
     5221 atattccaag taatatgatt gcatacggaa atccatgccg tattattcga gaaaacaaat
     5281 aaagttaaat tttcttgcaa aatctcaaaa aacacctttc ttactttata catagggtat
     5341 tttccaatac tttcacatcc atattcattt gttaaatttt tgtggaatgt agtttttatc
     5401 acttctatct tacatttcca ccatttttta taaaagtcat tcctttatag atttatttat
     5461 aatagtatat attctatttg atgtttgaat aagcacttat ttttaagtaa aatactaact
     5521 tttaggttag tattttgcta tagtatgatg atta
//
LOCUS       DAEPOG010000212         5498 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEPOG010000212 DAEPOG010000000
VERSION     DAEPOG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.212"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            154..888
                     /locus_tag="KR432_003340"
     CDS             154..888
                     /locus_tag="KR432_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0641848.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            2030..3286
                     /locus_tag="KR432_003341"
     CDS             2030..3286
                     /locus_tag="KR432_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0641849.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            3349..4542
                     /locus_tag="KR432_003342"
     CDS             3349..4542
                     /locus_tag="KR432_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0641850.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            4749..5129
                     /locus_tag="KR432_003343"
     CDS             4749..5129
                     /locus_tag="KR432_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641851.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         2048 a          555 c          951 g         1944 t
ORIGIN      
        1 caaaaaaatc aagtcgagta atccttcaac ttaagtgata tactaggtat taataatttt
       61 taattaataa aatataacta gaggaaaatc tacattttct caatttcttt aattaaattg
      121 acctctaatt aagtaattat aaggagaaaa attatgaatt tagcaaaggg attagatgtt
      181 ttaaaaattt catcaaatgt attcggtgaa gataaagtta tgtatattcc tgtaatctac
      241 acagaagatg atgcaacact tattgacaca ggtcttccgg gtcaaggtga tttaataatt
      301 gatgctctta ataaaagtaa cacgagcttt gataggttaa aaaatataat aattactcat
      361 catgatatag accatatagg aaacataaat tatctaagag aaaaatctaa gaataatatc
      421 aaagtatatg catacaaaag tgaagtcagt tatataacag gagaagaaac tccttttaaa
      481 ctttatatgt tggaacaaat ggtagataaa attgatgata agatgcttag tatgctaaat
      541 gttatggtac ttggatttaa atcatcatat actaaagtag atgtgtcatt agataaccat
      601 gaaaaattga atctaggtga ggaaatagaa gttatacata caggaggtca cactagaggt
      661 cacatatgtc tttatttaaa agagtctaaa gtacttattg ctggagattt gttacaggta
      721 gaaaatggag aacttacgcc tgttgatgta atgcacagca acaaacaaga gttaaaagat
      781 gctattaaaa atataagtaa ttatgatata gaaacgatag tttttagtca tggtggatta
      841 tatcaaagag acataattgg aacattgaaa aacctaatta ttgagtagta ctatatgatt
      901 tacaataatt tcttaaatta cttaaaatta tatctaaaga tgtagaatca aaattaaata
      961 taagtaataa gaattttgct ttaaaattat acttaagtgt taagtatgat acgtagtatt
     1021 acaagtattt tagaatataa ttttttatag taattttaag gttctacaat acttcagttg
     1081 aaaaatattt aacagcatag tataatagga aagaaaatat tttaaataaa ctttagtata
     1141 caaagtgaag aaaaatattt aaattatata taatattgta gagtgaggta aaatatttaa
     1201 gttatatatc ataatacaga gtgaagaaaa atatttagat aatgtaccat aatacaaggt
     1261 gagtaaaaat atttaaagca tgtataataa tgcaaagcga agaaaaatat ttaaataatg
     1321 taccataatg cagagtgagt aaaaatattt aaataatgtg ttataataca aagcgaataa
     1381 gattatttaa agcatgtatt ataatataaa gtgagtaaaa atatttaaat catgtatcat
     1441 aatataagca gagtgaagaa aaatatttaa ataatatatc ataatgtaga gtgaggtaaa
     1501 atatttaagt tatgtatcat aatacaaagt aaagaaaatc atttaaatta tatatcataa
     1561 aaataatatg aaatattgaa cactcataat attggaggct ttaatatttt aattgatagg
     1621 ttacattatt tataacatta aaaatacaat ttacataaaa tagacatatg aaaaataaaa
     1681 aattatataa taattatggg aatgaggttc tcccttggat gataatatcc taaactgcca
     1741 atgagctgat gacttctatg atatcataga tgccaccagc tttttttgtt gtttaaatcg
     1801 ataaaatgga ggtgaattac ttactaaatt tttatttttt aaatttgaag aaatatttaa
     1861 ttaatagaaa aaacagtaga ttatatgtta attattttat taagaatggg ttaagagtat
     1921 acctatatcc tatcaattta aaaaacaatt atgatagtca ttacattgac tacagttgat
     1981 gcaataaaac tttcaaaaaa tcttatgatg caaatagaaa ggaattaaaa tgatagaaaa
     2041 atttaaaact ttaatatgga ttaaaatgat aataagaatt aaaagcgttt ataaaacata
     2101 tggtggacta tttttgttag gactcatagg tataccagtt ggtgcaatta taggtcttat
     2161 agacaccata tttggaacgg ttcttctaaa agttacagat attcgagaaa catatccaat
     2221 gtatctgata ccatttttag cagtagtagg agttgttatt gcttattgct attttaaatt
     2281 tggaggaaaa agtagtaaag gaatgaatct aatatttgag gtagggcatg gagaagaaga
     2341 aattattcct ttaagattgg ttccatttat aatatcaggt acatggttaa cccatttatt
     2401 tggtggtagt gcaggacgag aaggagttgc tgttcagatt ggtgcaactt tttcacattg
     2461 ggtaggtaaa aggttgccta taaaaaatgc ttctagtatt tttctagtaa ctggtatagc
     2521 agctggtttt gcaggattat ttgagacacc aatagcagca atactatttg ctatggaggt
     2581 tttggtagct ggttcactgg agtatcaatc gctttttcct gcgtttactg cttcttttac
     2641 agcaagtgca gtatcaaaaa ctttagggtt agagaaattt tcttttgcac tttcttctaa
     2701 agttgtattt gacttaccaa tattttggaa gcttattgtt ttaggaatta tttttggaat
     2761 ggttggtgga gcatttgcat ggtgcctgaa attgtcaaaa agaaaaatag gtaatagatt
     2821 aaaaaatcct atgataagga ttgctattat tggagtatgt cttagtgtat tattcctttt
     2881 attttataaa ggaagatatt caggtttagg gacaaattta attcaaaata gtttttatgg
     2941 tggagaaatt tattcatttg actggctgtt aaagtttata cttacaatac tgactttgtc
     3001 agcaggtttt caaggaggag aggttacacc tttgttttcc attggagcta gtcttggtgt
     3061 attgttagca ggatttttta atttgccaat tgaattagtt gcagcgcttg gctatgctag
     3121 cgtctttggc agtgctacaa atactttttt tgctcctgta tttatagggg cagaagtttt
     3181 tggatacagc tatttacctt atttctttgt agtatgcgct atttcatata tatttaatat
     3241 ggataaatct atttattcac tacaaaaaat ttcaacgaaa caataaataa aacaacataa
     3301 tgtatgatta taagagaaag aatattagag aaaggatagg gtaggaatat gttgacaagt
     3361 ttagctttga tttttttatt aggaatggcc tcgggaggta tttttgagaa gataaaatta
     3421 cctagcttat tgggaatgtt acttacagga atcatattgg gttcatatgt tttaaatttg
     3481 attgataatt caatactaga tatttcttct gaccttagaa agattgcatt gattattata
     3541 ttaacaaggg ctggattatc tttagatata aacgatttga agaaagtggg aagacctgct
     3601 gtacttatgt gctttattcc agctactttt gaaattatcg gaatgatagt tttagcacca
     3661 aaattactag gcgtttctat attggaagca gcagtcatgg gtgcagtagt aggagcagtt
     3721 tctcccgcaa ttattgtacc taaaatgctt aaattaatgg aagaaggtta tggtacagaa
     3781 aaaagtattc cacagatgct tttagcagga acttctatag acgatatttt cgtaattgtg
     3841 atgtttacag tattcacagg attggcacaa ggaaatagta tatcagctat tagtttttta
     3901 caaattccag tatcaattat attaggtgta atagttggag cagtgattgg aatctgttta
     3961 gcagtatttt ttaagaaggt tcatatgaga gattctgcta aaggggtgtt gcttttaagt
     4021 atctcatttt taatgataag tttggagact gctttagagg gtattgtgcc attttctgga
     4081 ttattagcag ttatgaatat tgggattttc cttcagataa aatatcgtgt agttgcaaga
     4141 agattgtctg taaagtattc aaaactatgg gttggtgctg aaattctctt gtttgttttg
     4201 gtaggggcaa cagtagatat aagttatgca tttaaagcag gaattggagc tgtaatttta
     4261 atatttggag ttcttttatt ccgtatggta ggagtattct tctgccttat taagacaaat
     4321 ttgacaataa aagaaagatt gttctgtatg attggctata ttccaaaggc tacagtacag
     4381 gcagcaatag gaggtgtacc acttgcaatg gggatagcat ctggacaact tattttgaca
     4441 ttagcggttc tcgctatact tataactgca cctcttggag cttttggaat tgatgtaact
     4501 tataaaaaat tattaacttc tgtagaaggt aagagtaagt aagaataaaa ataaagatta
     4561 ttttaagtga taaattatat tttggaaaaa actatattat tgagtacaat gttagaaaat
     4621 catttgagtg attacagaag gtagaagttt atatcggatt ttaaggcatg tgatgattat
     4681 tagaattgat tttgtgaaat tgttattgta atatttaatt atatagataa agaagcggag
     4741 tgataactat gtatttaaaa aaattatcaa aattagagtt agttattatg aaatttattt
     4801 ggaatttaga tataaaaacc aattcatatg agattattga ttatatgaaa gaagagcata
     4861 atttaccaga aaaagtggct ttaaaaattt tatcaaaatt atcaaagaaa agattcttat
     4921 atgttcaaga aacaggcaag tgtatgtatt atacagttgc aataaaagaa aagacatatt
     4981 tagagtttat atcaagaaat gtgcaaaatc ttttgaagaa taattttata aggaatttat
     5041 tagtatcatt tcacgaagag gagctaacag aggaaaagat aaagtcgtta gaaaactggg
     5101 tagtaaattg ggaagaagct tatatataaa tgttattatt tttattacaa tttagataat
     5161 tttggttttg taagctctgc aactattttt agatggttgc agagcttatt tttatttttt
     5221 attcccttaa gcaatactta aaagtaaacg catttatatt tactaacttc aatcacttct
     5281 aatacttaga ttacttttta aaaatatttt gatatgtcat ctctatagta atctagaact
     5341 tgtttctatt ctttatataa agtttttatg aatgatatat ataatattcg ttcatttgat
     5401 taaaaataac aaaatattaa ataattctac tctgatagtt tgttaaaaaa ataataaaaa
     5461 atattaataa acaaaaaaaa aaattatctt aagagagg
//
LOCUS       DAEPOG010000213         5457 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEPOG010000213 DAEPOG010000000
VERSION     DAEPOG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.213"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..663)
                     /locus_tag="KR432_003344"
     CDS             complement(<1..663)
                     /locus_tag="KR432_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641852.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INF"
     gene            complement(682..1617)
                     /locus_tag="KR432_003345"
     CDS             complement(682..1617)
                     /locus_tag="KR432_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0641853.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            1825..4410
                     /locus_tag="KR432_003346"
     CDS             1825..4410
                     /locus_tag="KR432_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0641854.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            4422..5093
                     /locus_tag="KR432_003347"
     CDS             4422..5093
                     /locus_tag="KR432_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641855.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            5120..>5457
                     /locus_tag="KR432_003348"
     CDS             5120..>5457
                     /locus_tag="KR432_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641856.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIK"
BASE COUNT         2176 a          682 c          736 g         1863 t
ORIGIN      
        1 aaaatttata aattttatct ctaaagaaga cttatttata ggtataacct tatgtataag
       61 tcttaaattt actatgtatt gtctatgagt cataaagaaa ttaggaggta ggcgtttttt
      121 taaatcatca aagttctcat aaaattttat cgtatcttca tctctagtat ataaattaat
      181 agctcttgaa tttttctcaa acatgactat atcatcaaaa ttaatcatat gtatctgttt
      241 ccttatttta tatacaaaaa gagtatcttc tataaaatta ttttttgcaa ttttatgaga
      301 gttaatgtgg tctattgcta tattaattga atcaagtatt ttttcttttt taaacggctt
      361 aactataaaa tctataggat gtactttaaa gctctcaaga ctatagtcag caaaacctgt
      421 tacaaaaact atatgtatat ctttatctat agaaagaatt tctttggcag aatcaatccc
      481 atttttatat ggcatatcaa tgtcaataat caataagtct attttctcac tttttataaa
      541 ttcaattgct tctataccat ccttgacttt cttaacataa tctacatatc cggactcttt
      601 aactatattt gctatatcat ctcttacaat tttatcatca tcacaaatca aaacttttat
      661 cacagacttc tctcctccta actaataata ttttggtatt aaaattacaa attcagttaa
      721 aaaatcgtca ctatttacaa gtatactata atcatactta tctagtatat ttttaactag
      781 atataaccca aaacctctgt cttgcccttc tttagtgcta aatccatcca aaaatatact
      841 ttcgacatta tgtattttag gaccattatt ttttatggct ataatatact gaaattcatc
      901 ttcatatgca tgtaatctta tatacttctt ttccttttgt atagcagaca aagattctat
      961 tgcattgtct attatgttta atagcactga actcatatca ttaggttcaa tctctaaatt
     1021 atccataaaa tcttggatat atacatcaaa agatatgttc ttttcaagac attcctcata
     1081 ttttaaggaa gctatagcat ctaaataaga tactttccca tatcttgaat tttttgattt
     1141 aaagctattt gatatattcg ttatatattc ttttgcatct tcagctaacc cagtatttaa
     1201 tagagaatat actacttgta aatgttttaa atagtcatgt ttttgacttc ttaattttac
     1261 gattatattg tccttatgat ttattttact gtctaatttt tgtagcttat actcttgttt
     1321 attgttattg tctagtaatc ttataaattt aaatcctata taaaagctta aaataattaa
     1381 aattaaaaaa agtataatat ttaatactcc ttcaagtaaa aaattatatc ctaaacttaa
     1441 ttgtatatac tcaataccct ccatgaataa aatccataca aaacacgata caaaaaacgt
     1501 taatattcct ctttttaata tgtctaattc tttataaaac ctgtatttat tcattataag
     1561 attactaaat ataaacaaag ttagtattac tataggaaat ataaaattat tctccataat
     1621 tcccctccca tctacacttt atatgaggat tatatcattt tagaaaaatt atgtaaaaag
     1681 tcaaaattta catgtacatt aattaaactt gcaaatacac atataaattt gtacaattaa
     1741 gtactcaaaa cttgaagata cttactttga tatatatttt aaataaacat ataattgatt
     1801 tagctaagaa aggaagtggt aaatatgaaa gataatttaa aaatagcact aagatacata
     1861 ataagttata aagcaaggtc tttagcaatt gctctaagta taattcttgc tacatcactt
     1921 attgttggta ttggtaccct ctcaagaagt gctcaacaag cagaggtaga taaattaaaa
     1981 agagaaactg gaagtgatca tgtatacttt aaagatataa ataaaaatca attagaatac
     2041 ataaagtcta gaaaagatat taaaaattta ggtataacat ctcactatgg ctatacagat
     2101 cctaatgaaa gattagctat aaacctagaa tatgcaaata aaaattactt gactaatcaa
     2161 tccaaactta taaaaggtca ccttccaaaa gctagtaatg aggtggtcgt agagaaatgg
     2221 attttaaata gtctgggttt aaaaccagaa ataaatcaga acattacttt taaattatat
     2281 caaaaagaaa aaccagagac atttaaagtt gtaggaatac ttgaagatag acctattgag
     2341 aaaaataaag ggacatgtga aattttttta aatctcaatg aatcaaaatt ggataaattt
     2401 tcttatgctt atattgaatt taataatggc attgatataa atacaagcat agataatata
     2461 gtaaaaaata ctatgttgga tgagaatagt gttggaaaaa acaaaatgct tatagaatct
     2521 actagggaaa atggtacttt agataactca agtaaataca ctgcaattac tatgagccta
     2581 ttttctggta ttgtaatata tagtatctat gttatatcga tatatcaaag aatacaggaa
     2641 tatggtatct taagagctat tggatccact aacttcaaga ttttcaagtt tatgttttat
     2701 gaacttttca tacttgcttt gatagcaata cctatcggta tatgcacagg tattggtggt
     2761 gcacaaatat ttaataggac tgcaggaaat attcaatttg aaggtaatgt aacagttacc
     2821 ccttttgtta ttccagataa aataatactt ttatcaattg gaagtataat actgacaata
     2881 ctaattataa gtttctttac atatttaaag ataagaagaa tttctcctat tgactctata
     2941 agaaaaacct ttggaactga taaaaatata aataaggtca attctttgat atcaaaatta
     3001 actttaaata tatcagtaac aaaatctata tctgctaaaa atatctttag gaataaaaag
     3061 ggcttcatta taattatatt atccatgagt ctaggaggta taatgatcat aaaggagaat
     3121 tataaatatt ctttttcaga tatacaaaat aaaaatggtc aagaagaaac atatatgaat
     3181 gcagatttta ttctaacaaa ttttttctta aaactaaatc aatctaataa agctgattct
     3241 ttcaaggata taaaaggact taatgataat cagatagata aaataaaaaa tataaatgga
     3301 attgacaaag tcaaaactgc cagcatctta gatacaagaa tagaagtaga taaattaaat
     3361 ggattagatt actacaatat tattaattca acgccttatt ataaggattt tcctctattc
     3421 ataaaaaata agaatactgg aaaatataca atgaaacaaa agttaagagg ttacaatgat
     3481 gagatgataa attcattaga aaaatactta gtgagtggga gtataaatct ggaaagaatg
     3541 aaaaaagaaa acctcgctat attatacgta ccacaagttt ctaaaacaaa taaatacaag
     3601 ctaagttata ctcctggtac agaaacgcct gctgtagata taaaagtagg agatactata
     3661 aaggtcaaat atccaaaagg tgagatagat caagatcttt atataaaact aaaagataat
     3721 tatgaatatc tagaatatga gtttaaagtt ggagctattg taagttatcc ttttgcagat
     3781 aattacctat attctggaga tcagggtata gatgtaataa caagtaacga ctatctaaaa
     3841 aaactaactg gtgtagataa atataatgta gtttatgtag atattaataa aaatgcaaat
     3901 gtaaaaaaga taaatacact cttaggtgaa ataggaagtg aatctcctgg aactacaaca
     3961 gctaatatgc taatggaaaa agaaaacttt gataagatga cagctagggc tctgacttat
     4021 gcttatggaa tagtagcagt aatgtttata attagtgtgt ttaatataat aaataacata
     4081 agttataact taacttcaag aactagcgaa tttggaatgt taagagctat tggtatctct
     4141 gagagaggat ttaaaaatat gatactctat gagggaatat tatatggaat actctcaagt
     4201 gtaattacca ttgtggtagg gcttataata cagtttagaa tgtattacac gtataatttt
     4261 gtatcatatg gattgggatt ttcaatagat tacaaaatat atatcctagt tgttttagca
     4321 aacataattg taggtattct agctacatat attccattaa ggaaaataaa taaaattagt
     4381 atagttgaag ctataaacat tacagaatag gaggattaat tatgcctata ttagagattc
     4441 ttaacttagg taagatttat ggaaaaaagg aaacagctgt acatgcacta aaggatgcaa
     4501 atttaaaaat aaataagggt gaatttgttg cgataattgg cccaagtgga agtggaaaaa
     4561 gcacatttct acatttagta ggaggtcttg agagaccaag tagtggaact ataaaagtcg
     4621 atggtaaaga tatatgttgc ctttcagata aaaaattagc aaaatataga agacaaaaag
     4681 taggctttgt ttttcaacag tataacctaa ttccagtatt aaatgtaaaa gaaaatatag
     4741 aattaccact aaagttagac aataaaaaag ttgatgaaga atacatcgaa gacattataa
     4801 atttattggg tcttagtgag aggaaaaatc atcttccaaa tcaattatct ggtggtcaac
     4861 aacaaagggt tgctattgca cgcgctttat ctgcaaaacc aagtataatc ctagcagatg
     4921 aaccaacagg aaatttggat agtaaaacta cggaagaagt catggattta ctaaaaagtt
     4981 ctataaaaaa atataaccaa acacttataa taataacaca taatgaaaat attgctagaa
     5041 aagcagatag aattatatct ataatagatg gagagcttaa attaacactc taaaactatt
     5101 tatagaaaag aggatttaaa tgttattgat tttagataat ttaaatttaa acttagattt
     5161 tgactacaga ataataagag aagaaaatga tgatgtagat atatttatag atattaatta
     5221 tcgtagctta gatatagata ctgatggttc taacttattt aattctagaa tacaatttcc
     5281 atttgtaaga gcattgattc taaggataaa taagaacaat caatgtatga caattcattt
     5341 gttaagagat atagatttat tctctgcttt tgccaacttt gaagtggatt atactgactc
     5401 tataataaat ataaaaaatc aaaatgagaa agtaatttta aataaatcta tcaaaaa
//
LOCUS       DAEPOG010000214         5448 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEPOG010000214 DAEPOG010000000
VERSION     DAEPOG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.214"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..812)
                     /locus_tag="KR432_003349"
     CDS             complement(<1..812)
                     /locus_tag="KR432_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0641857.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITK"
     gene            complement(812..1366)
                     /locus_tag="KR432_003350"
     CDS             complement(812..1366)
                     /locus_tag="KR432_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0641858.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(1359..1961)
                     /locus_tag="KR432_003351"
     CDS             complement(1359..1961)
                     /locus_tag="KR432_003351"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH0641859.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(1970..4618)
                     /gene="polA"
                     /locus_tag="KR432_003352"
     CDS             complement(1970..4618)
                     /gene="polA"
                     /locus_tag="KR432_003352"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH0641860.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            4937..5019
                     /locus_tag="KR432_003353"
     tRNA            4937..5019
                     /locus_tag="KR432_003353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4971..4973,aa:Leu,seq:caa)
BASE COUNT         1646 a          961 c          562 g         2279 t
ORIGIN      
        1 tattttgtta tattaaactc tttttcttga aacttgctta aatcatttaa tgtaacattg
       61 gttatatcac tatcaagagc cataaccata gatgtccttg cctcttcatt aggcaccata
      121 cctactttta tgttcttcat ggtctttgga acttcttcat agtaatcttt gtttacagat
      181 aaaaccattc cttctctttc tgtatatgat tctattttat atttcccatt tcctatcata
      241 ttattattct tgtcatttac atcctttgaa tctaaatgat tttgagatac gattggaaaa
      301 attaaactat caattgaaaa agcatatcta gctttaaatt ttattgtaaa atctttatct
      361 gatttaatat tcacagaagc tatattttta gtaaattcat tatatggact atctgcactt
      421 ttttgaatta aaccaatagt atagtttaca tcatttgaag taacattttt tccatcatgc
      481 cactttgcat cttttagctt tatatctata ctcattccat cctgagctat attatactcc
      541 ttaactaatt gaggaattac attgtagttc tcatctatag taaataaact atcataaact
      601 aaattcatta tataaccaac agacttatca gtattagtta taggatttat agtttttgga
      661 gttaccattg ttaagtttat atacttagca tctatagaac tttgactttg taaacttgaa
      721 tcactactac tttgttttgt tttagtatta ctacaaccag ctaccataag agttatagct
      781 aaaacgactg ttaagacttt tattctcttc attagttttt tcctttcttg ccatatatct
      841 tattataatg ttcgtaatta ttttttactt tttctacata ttttgaagtt tgcttaaaag
      901 gtatatcatg tagattttcg ccatctttac tatattcatt attttctaac cattttttca
      961 cattacctga accaccatta tatgcagcta tcactaaatc taatttacca aattctttgt
     1021 ataacttact taaataccaa cagccaagtc ttatattagt ctctgggtcg aaaatatcca
     1081 cattttttaa atttagttcc tctgctcccc aatctctagt tatatctaat atttgcatta
     1141 atccttttgc ttcctttttt gaaacagcag aactattaaa cttactttcg gctttaataa
     1201 cactgtaaac tatattttca tctaaattaa attcttttga atacttttct acatactctg
     1261 aatatttttt aggatataaa aatttatgta ttactttgct ttccattaat agtgccccaa
     1321 ataagattat aaaaatagat agaatcaata ctttcttact attcacgcca attctccttc
     1381 atgtactcaa taaatttatg tgctttactt tctaattcag ttattgttcc cgaattatct
     1441 attatataat caccatattt ggatttttct tcctgactca tctgtgaatt tatcctacta
     1501 agtgcttcct gcttactaca attatctctc ttttgtattc tactaatttg tacttcttgt
     1561 ttgcaagtaa ctactaatag cttgtcaacc atatctaaaa aaccactttc taccaatatt
     1621 gctgcatcta atactactat attttcgcct ttttttctca attcttctat ttcacttatt
     1681 attttttctc ttattcgcgg atgagtcaag ctatttaatt ccttaagttt ttcttcatcg
     1741 ctaaaaacta ttttcccaag agcttttcta tttagagttc catcatcatt ttttatactt
     1801 tgcccaaaat ggacaaatac tttttccaat aacaacttat cctcaaatat cttcctggat
     1861 ataatatcag catccactat agaaatatta agatttctaa atatatttga taggctgctt
     1921 tttccacagc ctattcctcc tgtaagtcct aatatcaaca taaaatcacc tactttgtct
     1981 cataccaaga actacctaca tttaaatcta catctaagtg aacatccata tttacagcat
     2041 tttccatttc ttcttttact attttcttaa ctatatctat ttcatcatca attgcttcaa
     2101 ctatcaactc gtcgtgaact tgtagtataa gttttgattt caaattgttc tcttccaatt
     2161 ttttatgaac atttatcata gcaattttta ttatatctgc tgcacttcct tgtattggtg
     2221 cattcattgc aaatctcttt cctctatttc tttccataaa attgcttgac tttatttctg
     2281 gtatatatct tcttctgttt aaaattgtta cagcatatcc atcctcacta gccttttcta
     2341 ctgttgtgtc cataaactct tttattttat ggtatttagc aaaataactt tcgatatatt
     2401 ctttcgcctt tggaacaggt atatttaaat catcagctag accaaagtca cttttaccat
     2461 atataatacc aaagtttaca gctttagctg cacctctcaa ttcactagta acatcttcca
     2521 taggaacatt aaaaacttgt gaagctgttt tggtatgaat atcttcatga tgtttaaatg
     2581 cttctatcat attttcatcc tgactcatat gtgcaagaac tcttagctct acttgtgagt
     2641 aatcggcatc aaccaactta cagttatcat ctgcaataaa cacttttctt atttttctac
     2701 ccatttccat ttttataggt atattttgaa gatttggttc tgtagatgaa attcttcctg
     2761 tagtcgttat agtttgatta aatgatgaat gtattctatt actttctgga tttattatat
     2821 ttaaaagtcc ttcaacgtaa gtagaattta actttactat ttgtctatac tcagttatct
     2881 tatctattat tggatgctta tctcttaatt tttctaaaac atctgcatta gttgaatatc
     2941 cagttttagt ttttttaatt attggaagct ctaatttttc aaataatata acacccaatt
     3001 gttttggtga atttatattg aatggctctc ctgataattc ataaatttcc ttttctagtg
     3061 tagctattat ttctttaaac tctacactca attcatccaa tatacttttg tctactttta
     3121 taccttcata ctccatatga cctaagactt ctactaaagg catctctaca tgataaaaaa
     3181 gaccatccat actcatatcc attagacttc tttccatcat aggaattgtt tctttaactg
     3241 tgcatattat ttgacccata tactctgcta aatcctcaaa ttctaaatcc tcatagtttt
     3301 tagatttaac accctttcct aataattctt cttttgattt aacctttttt gataaatact
     3361 tcatagctat tgcactacaa tcatatgagg tagatgaagt tgaatctatt aagtactcag
     3421 ctattgttat atcaaaatac atattttcta agtttatatt atatggtttt aaagatatat
     3481 aatcatcttt taaattatat ccatactttt taatttcatt atttgaaaat atactatcta
     3541 actttgacac ttcatttgaa tctacataat atattttttc tccatctaca cttataaaaa
     3601 tatatattat atttttttca agtatattac cttgtcctct aacagttttt aaaataactt
     3661 gtcctttttt gtttacttca tctataaatt tttctgtatc ttttaatttt aatatttcta
     3721 ttttttcttc tacgtgttct gtgtcatctg aatcgaccaa atcaactaat cttgataaga
     3781 gacttgtaaa tccaaagtat ctaaatttat ctaaaagttc atctttatca taatcgccaa
     3841 aaaccatact atctaaatcc acttctattg gcacatctct tataattgtt gcaagtcttt
     3901 tactttgaat agctatttct ttattttcct ctattttctt ttttacacta cctttaagct
     3961 tatctgtatt ctctattaaa ttctctatac tagaaaactc ttttataagc tttatacctg
     4021 ttttttcacc gattccaggt actccaggta tattatctga cttatctccc ataagacctt
     4081 ttaaatcaat aaattgtgct ggagtcatct cgtatttttc aattacagaa ttaaagttgt
     4141 attcttcaac ttctccaact cctttttttg ttataagagt agttgtatta tctgaagcaa
     4201 gttgaatagc atctttgtct ccagttacta tgtatacctt atacccatta ttttctgctt
     4261 ttttagaaac agttcctata atgtcgtctg cctcataccc atctatttcc aatctattta
     4321 ttttgaattt atctaaaagg tcttttaatg gctgtaattg ttctgccagt tcatctggca
     4381 ttccctttct tccagcctta tactctttat attctaaatg tctaaatgta ggtgcttttc
     4441 tgtcaaaggc tacacttata tgtgttggtt tatatatatc tatcatttta aatagcattg
     4501 ttgtaaatcc atatattgca ttggtcttta acccttcttt attattcatt tctggcagag
     4561 cataaaaggc tctgtttatt attgaatttc catctattat aattaatttt ttttccaaat
     4621 tatatcactc ctaaaaatta tctatttttg taacatatat attatatacc aatatagtgt
     4681 tatttaatat tcccataata ataatagtat attaaaatta aatatgttgc aaacaatata
     4741 tttttttgct ataataatat acatgacatg cccgaatggt tgaattggta aatacaatgg
     4801 attcaaaatc aactgtgttg attcataagt ttttataatg ttttacagtt tgatgagtat
     4861 tttgtattca atattcctct tgcatattct tggaaaatct ggtaggatat acaagtagtt
     4921 tggatttaaa atataagccg ctgtggcgga actggcatac gcatacgact caaaatcgta
     4981 cgggaaacca tatgggttcg agtcccatca gcggcaccaa taaaaaaaac tctttagtat
     5041 atactaaaga gtttttttat tgtaatattc tttcatcttt atttctagcc ttgtataagt
     5101 ataaatacca tagtcataac ttcttgaact cctaaaatat atctaaacaa agtaggattt
     5161 tttatgtatt cacttaataa atccatactt cttatcatat tgattttttc ccaatctcta
     5221 aaaattttga tacaaataaa aaatacagag aactacttga tatactagaa ggttattctg
     5281 taccatttgg tattgctgta ggtgttccag atggaaattt accaaatgct agagatgtag
     5341 tttataaagt ttctagagtt taggataaag tgttgcaaca atcaattagt tacttgaaga
     5401 gttaaatcag taatatacaa ataaattaat ggaagaggaa gctattta
//
LOCUS       DAEPOG010000215         5440 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEPOG010000215 DAEPOG010000000
VERSION     DAEPOG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.215"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(231..1115)
                     /locus_tag="KR432_003354"
     CDS             complement(231..1115)
                     /locus_tag="KR432_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0641861.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(1406..1882)
                     /locus_tag="KR432_003355"
     CDS             complement(1406..1882)
                     /locus_tag="KR432_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH0641862.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            2149..3324
                     /locus_tag="KR432_003356"
     CDS             2149..3324
                     /locus_tag="KR432_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBH0641863.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(3405..4208)
                     /gene="proC"
                     /locus_tag="KR432_003357"
     CDS             complement(3405..4208)
                     /gene="proC"
                     /locus_tag="KR432_003357"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0641864.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4248..4832)
                     /locus_tag="KR432_003358"
     CDS             complement(4248..4832)
                     /locus_tag="KR432_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641865.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         1811 a          901 c          612 g         2116 t
ORIGIN      
        1 ctttaaaagt aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc
       61 catattgaga aattttacta aactattatg tactttatta aataaataaa ataacagcac
      121 acctataaaa tacaagtgtg ctgttcataa acagatttta taaaaaaata attcctaagt
      181 agagattaac atgctttctc atactctcat atataatgtg tctaaaaata ttaataaaaa
      241 tgctttaatt gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc
      301 tgtcttattc gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg
      361 tcatcatcat caattccaaa tctcattctt aaaacttgct gttcttgctc accaagacct
      421 gtaagtagtt cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca
      481 tctttaaaat cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt
      541 aatggagtat ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca
      601 acttctaagc cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt
      661 aaaagttctt gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc
      721 ctaatagtat tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt
      781 gcataagtgc taaatttgta tcctttttta taatcgtact tttctacagc ttttataagt
      841 cctatatttc ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt
      901 gctatgctca ctaccaacct ataatttgaa tttataaatt tttcttttgc tactgaagat
      961 gagttgatta tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc
     1021 tctatttcct ttaaatacat tttcatcgca tttgatacat ttgatgaacc cacacaaata
     1081 taattttcat aattattttc ttttttagta tccataatca aaccccttct attcatagaa
     1141 tttgctaaaa caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca
     1201 ttctttaaat gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa
     1261 tattattcta cttctaaccg caatatcctc cttatatata taatttattt tattatatgt
     1321 tgttatattc ttagtttttt gaatgatata tgtctaaaaa aagactgttt actaaacagt
     1381 ctttttttat atatatgctc aatatttaat tttcatgatg atgattacat ccacatccac
     1441 aatttttatc atggtgatga tgttcttcta aatcttttgc ctgttgttcc atttcatgaa
     1501 actcttttac cattatatca taagttggaa gagctgcctc agtatcgtat tcttcccctc
     1561 tccaatcagc gtacatcata gcatccccat tagttaaaac caatctacct gacatagtta
     1621 tattatccac ttcttctaaa aattctattt gtttttgtaa aacttctcct tctaactcta
     1681 catcttcatc taaacattgc actatcatta caggtgcata ataatcttct tcatctttaa
     1741 catttacaac aatatctaaa actccttctg caccttcttc aaattctgcc aactcaacat
     1801 ttgtatcaat catatcttga gggactaata aatcttctat taaatatttt attactctat
     1861 cttttactaa ttcttttgac atttattttt ctccttccga ttctataaca ttatcataat
     1921 catttctata ttatagtata ctacattaat tgttttttca atctcttaca aaaattattt
     1981 ttttattatc ttccttatat tccctttttt attacaaagt tgtaatcgca ttgatttaaa
     2041 aaatagttta tatttagtat aatagaaatg tagcacatat ttttattcaa tatattaata
     2101 aaaaaataga ttgaaaatgt aatgtatgtt aaatctagga ggaatattat gaagtttaaa
     2161 aaacaaatta gttctattgt tgtgtctact atgcttgtac ttgggagtat gaacctttct
     2221 tatgcagatg gtacaaatgt agttacactt ggagctaact tatcagagtc tcaaaagcag
     2281 caaatgctta attactttgg agtaaaaaaa gaccaagtct tagttttaga tgtaacaaat
     2341 gcagaagaaa gacagtattt acaaggtgtt gcaacagagg gtcaacttgg aaaagttact
     2401 atctcttgct catacgttga acctactaaa aaaggaaatg gaattaatgt taaaacagca
     2461 aacttaactt gggtaactag ttcaatgatt gcttcaaccc ttacaacagc tggtcttgaa
     2521 gatgcaaatg ttattgctgc tgctccatat cctgtaagtg gtactggagc attgacagga
     2581 atcatgaaag catttgagga tgcttcagga aagaaacttg atgaaactaa gaaagaaatt
     2641 gcttctgaag aattggtagt tacaggtgac ttaggagatg atatagggca agaaaaagca
     2701 acaggtgtaa tgaatgatat aaaaacagaa attgtaaaga atggtacaaa agataccaat
     2761 caaatagcaa atacaataaa taatgttgta aataattaca atattactat tacaccagaa
     2821 caaaaagaac aaattaaagg tgtaatgaaa aaaatagctg accaaaatta tgattacaat
     2881 agcatgaaaa atacactaga taatgtatca gataatgtaa atgaacaatt aaagaaatta
     2941 ggtgaaagtg ttaagggttc tggtatacta gatactattg gtggatggtt tagtggaata
     3001 ggagactggt tctctggttt attctcaggc ggagataaaa aagatttggg aatcttaaat
     3061 aacactaatg atgagtctct tggctctgat gctgtaataa attctacaga aggtgttcaa
     3121 ataaatccac aaaataatac taattctaac aatgatagtg atacaaatag ctcaaaaaat
     3181 gatacttcaa gtaataatga aaataacgat agttctcaaa caaatgaaaa caacacaaca
     3241 aataattcag aaaatcaatc aaataataat gactcagttg gtttctttga aaaaataaaa
     3301 aattggcttt caggcttatt ttagaaatta aacaaataaa aagaattgcc taaaatggaa
     3361 attttctata aaaggcaatt ctctttattt acaataaatt tttattactc actcattttt
     3421 ttagattttt ctatacatgc acatattgct tttatcacag aagctctaaa accttcttct
     3481 tcaagtactt taactgcttc tatagtagtt ccaccaggtg aacataccat atcttttaat
     3541 tcaccaggat gttttcctgt ctcaagtacc atttttgcag aacccataac agcctgtgat
     3601 gcaaacttat atgcttgtgg tcttggcatt cctgcaatta ctgctgcatc tgctatagct
     3661 tctataaata aaaatacata tgctggtgca gaaccacttg caccaattac agcttctata
     3721 agatactctg gtactacctc agtatttcca aaagaattaa atacttctgt aactgcttct
     3781 aagtcttcat tgtttatatt tttatttata gaaagagaag acatggcttc atttacaaga
     3841 gctggagtat ttggcatagt tctaactatt tttttatctt ctcctataat tgactctata
     3901 tcctttatag attttcctgc tgctattgta actataattt tattgtcatc tataaagtct
     3961 tttatctctt ccaaaatatc atcatatata tctggtttaa cagctacaat tactatatca
     4021 gaggttctag ttacttcctt cgagtctttt gtagtattta ttccaaaatt tgcatctatt
     4081 ctatcgagag ttccttgact atatgctgat gctataacca tattaggttc aactaatttt
     4141 gaatttacta ttccccctat catagcgctt gccatatttc ctgcacctat gaatccaatt
     4201 tttttcactt tagtacctcc ttattgacta atagtaatcg ttaaaattta agcttcttta
     4261 actgcatcag acttttcaaa tggatttggt atacttacta ccaaaatttt cactacctta
     4321 ttagtatagt tgacccaatt atgattgttc ttttcactat tatagtgtat tgtgtctcct
     4381 ggatacattt ctatttgttc atctcccaaa aacactgtaa gagtaccctc taatacatac
     4441 acaaattcct caccttcatg tacgtaacaa caaatttcct cactactctt acttggaaga
     4501 atttcaatta atcttggtag cattgttttt tcctttaaac tagatgataa atgataatgt
     4561 ataaatgttg agttttctac atcaaatact tctttttcat aacttcttaa tacagctctt
     4621 ttatgctctt ttggcttcat aaaaaagtaa gttaattcta catctagtac acttgctatt
     4681 tttcctaagg aatctgttgc cacacttgtt aatcctcttt caagttgtga taaaaaacca
     4741 attgacaagt ttgttttttc actcatatcc ttcagcgtca tttgcttttg agttctgagt
     4801 tgctttattt ttgctcctat gtccttattc aaaaccatca cccctaaata attttaattt
     4861 tttcataata taaatacaaa aatataaata taggtactga acctatcttt atttcattta
     4921 aaaaaaattt ttataagtat tactataatt tatcaatctt tttttgtcta catttttacg
     4981 attatttacg atttattttt cacatatgta tatttaatct tgccaactaa agatacattt
     5041 tttataagca tttactcttt atataataaa acttaagttg ttcatgtagt cttcttctgg
     5101 tttaattatt aaaatttcaa attatattat tatttctaca cattattaca tcctttagta
     5161 caattataac ataattttac aatttaatga tatctattca atgtcttttt ttactctaca
     5221 acatatttta ttttagagtt taatactata tatatttttt atatacagcc ttgtcatttt
     5281 ttagattgtc aagagttatt gaagtatttt atgtagattt atattttatt ttctaatttc
     5341 tcatttaaca agaattgctt atgataaaaa ataatatcat ctcaataaga ttcttgtaaa
     5401 tatatctata ttctaaatat aatttctttt tttgtcaaaa
//
LOCUS       DAEPOG010000216         5314 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEPOG010000216 DAEPOG010000000
VERSION     DAEPOG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.216"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..260
                     /locus_tag="KR432_003359"
     CDS             <1..260
                     /locus_tag="KR432_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0641866.1"
                     /translation="ILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQLRYGL
                     TPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(357..2021)
                     /locus_tag="KR432_003360"
     CDS             complement(357..2021)
                     /locus_tag="KR432_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBH0641867.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(2093..3340)
                     /locus_tag="KR432_003361"
     CDS             complement(2093..3340)
                     /locus_tag="KR432_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0641868.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(3327..4001)
                     /locus_tag="KR432_003362"
     CDS             complement(3327..4001)
                     /locus_tag="KR432_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBH0641869.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(4173..5252)
                     /gene="mltG"
                     /locus_tag="KR432_003363"
     CDS             complement(4173..5252)
                     /gene="mltG"
                     /locus_tag="KR432_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBH0641870.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         1598 a          945 c          563 g         2208 t
ORIGIN      
        1 atatacttgg cacagaggct aactatgttc ttgatgaagt tgaactaaaa gttcaagttg
       61 gaaaacttta cgaacaacta aacaaaattc ttactcctag agaaagagaa attgtccagt
      121 taagatatgg gttaactcct tatggctata agacacaaag agaaattgca caaaaactcg
      181 atatatcaag gtcatatgta tctagaatcg aaaaaaaagc tcttaaaaaa ctagaaaaag
      241 agcttgttca agaaagttaa tactatattt taaaaggata tatcgtacat cattagacaa
      301 agttttctat ctaaaatcaa agttacgata tatccttaaa ttttatagta cataatttat
      361 tttatacttt cacatatttc cttaaatata ggtgttgcag atttatttcc tttttgagtt
      421 ccttctacta acacagttat tacatactta ggtctttctt caggataaaa tcctgttatc
      481 catccgtggt ctatagcttt tttatttaaa ctactttgag ctgtaccagt tttaactcca
      541 caaccacctt caatatcttt taggtcttta gctgtaccaa tcctagatac agacttcata
      601 tattctttaa cttgtgtaca cacataagga gaaataagtt cttctttttt tgaagactta
      661 taagttttta ttgtattcat attattgtcc acaagacttt tatataagta tagtggtttg
      721 aatgttccat tattagctat tatttgagtc atttgattta tttgaagtgg tgtaaattct
      781 atattttctt gacctatagc taaatttctt attgatatgt tttttggagc ttctctaatt
      841 ttttcttcat caagaccaat atcaaccttt tcaaataaat gtaatttttc agcagatttg
      901 agtatctttt cttttccaag tttcatagct atatctaaaa atgcaggatt gcatgaatta
      961 gaaaaagctt gtcttaagtt ttggaaacca tgaccatctc ttttattaca tcgcagtatt
     1021 tcattagtat tcccaacttt agtcttacct gtacagttgt aagtatagtt ctcatctata
     1081 acaccatttt caagtgcaga aaataaaact accatcttaa atacagaccc tggtggatat
     1141 gtcgctttta taactctatt ttcaaattcc ccattttttc cttttaaact ttttgatatg
     1201 tcattttggt caaaattagg tctagagcac attgcaagaa tttcacctgt agatgcttcc
     1261 gagattattg ctgctgtagg attttcttct ttatttagta tttgttctaa ttttttttga
     1321 atgttataat caatagtagt ttttagatgt ctatctttat ccttattatc aacagttttt
     1381 acacttccct ttagtatgtt tagagatttt tcatttcctg catctccagc tttaaaagca
     1441 cttatatact tttcatttga atttctaagt acactatcca tactcttttc aattcctgat
     1501 tgacctgatt tatcatcttc ttttatatat cctattgtat gtgataataa accatctttt
     1561 gcgtacctca tagttttttc ttctaccatt attccatttt tctcaagttc ttttttcata
     1621 ctcttatcga tatttttagt ctgaatctgt attatagggc gtgtcaattg ctcttgtaca
     1681 gctttatata tatctaaatc attcatctta gtagctttct taataagttc caaaatttta
     1741 tagttattat ttaatttttc tttttcaaca attaatacct gagatttact tgtatctgta
     1801 agcttcttgt tgtttctatc atagattatt cctcttccac tatttaactc gactttctca
     1861 acgctttgtg attcaacact ttgtttgtat ttatcacctt ttaatacttg tatatcaact
     1921 agcctataaa ttaaaacact ataaattatt aaaattatag taaatataca ccagcttctc
     1981 tttcccactt ttttcaaaaa tggagtcttc ttttttgaca tgaaatcacc ccttagataa
     2041 tttcattatt gacaatttct aaggggctaa tactattttt atcaattttt tattattctt
     2101 caaccttttc ttctataact tttctaagca tataatcttt accaactttt actgatagct
     2161 ttaacttcac tatttgtcta ggatgaggag ctgattctat agcttctcct tcttcattgt
     2221 acatttcttc aattttagta aacatagttt ctttatatgg tcccataact tctatttcat
     2281 caccaacaca cattttattt ctttgctcaa caacaataag accatcttca tcttctgttt
     2341 ctctaactat acctataaaa tcatagtttc taacatatga tgctgattga taattttggt
     2401 cttctgttgt tggtttattc aagtaaaaac ctgatgtaaa atgtctatga ctaccttttt
     2461 ttagttcttc catccacatt ggattgaact tccagttttc tggggctctg taaaattcat
     2521 ctattgccat tctgtatgct cttacaacag tagctacata gtatgcagtc ttcattctac
     2581 cttctatttt taaacttgtt ataccagatt tgattaactc tggtatatac tctatcatac
     2641 ataaatcttt tgagttaaag aagaatgtac ccctttcatc ttcgtatatt ggaaaatact
     2701 caccaggtct tttttcttcc accaaatgat attgccatct acaagattga gcacaagaac
     2761 ctttgtttgc atctcttcca gtcatatagt tgcttattaa acatctacca gaataagaaa
     2821 tacacatagc accatgcata aacgcttcta tatccatatc taaaggtgtt tttgctctta
     2881 tctcagatat ttcatcaaaa gataattctc ttgccactac aactcttttt attccatgct
     2941 cgtaccagaa gtttgcactt agataattgg ttgtatttgc ttgtgtactt aaatgtattt
     3001 ccatattcgg aattactttt ttaatgacac taagcactcc tgggtcagct attataactg
     3061 catctattcc tatctcttct agttccaaca aatagtcttc taattgtaga aaatcttcat
     3121 tatgaggtat tatatttact gttacgaata ctttcttgcc tctttcatgt gcaaaagcta
     3181 ctccctctgc catatcttcc ttactaaaat tcttcgctgc agacctcatt ccaaaaattt
     3241 ctccacctat ataaacggca tctgctccat atgttatagc tattttcaat ctctccaaat
     3301 cacctgctgg agctaaaagt tctactttat ttaattccat ctacatcacc tcttttactt
     3361 tctttataac ttagtgctac accatcacct attggtacca atgatgtgct taaatagtca
     3421 caattacata tataatctaa ataatttctc attcttttaa ctatagtctt ttttcttctt
     3481 acaacaaaat catcatgagc aaccatacct ttatacaaaa tattatcaga tactaaaagt
     3541 cccccatctt ttaatttatc tataactaaa tcaaaaaata gcttatattg acctttggct
     3601 gcatctataa atataaggtc aaattcacct tggacttgac taagtttttc ttctgcatct
     3661 ccttctagta tagttatgtt attgtcaaat ccagctaatt ttatgttttc ttttgctttt
     3721 tctatcattt tttcatttct ctcaacagtt attatatctg catcttttcc tataatactt
     3781 gcaaaaaaaa tagaagaata accaattgca catcctactt ctaaaattct ctttggtttt
     3841 tgaaccttta acaagacttt taataaatct gaaacttctt tatgtattat aggaacacta
     3901 ttttcctctg cataaatttc taaatctttt aaaaatcctt ctttttcttt taatgtcact
     3961 cttatataat cttcaaccaa atcattaact atattactca taaaaacact ccctaaaata
     4021 ataaatattc aagttttata ttaatttatt attttttaaa ttcatattgt tatatatatt
     4081 tacactttct ttattataaa tctaagaata acaattttca ataaaaaagc ccaacattat
     4141 tcttaccctg ttagactttt tataatcatg gtttattttt ttgtattttc tttatttgtt
     4201 gcttttgtat cttttgattg tttatccctt gcttctttat attctttaac gtattttaaa
     4261 tggtcttgat agtttgtact ataattattt ccaccatcaa tcttagctac aaaatataaa
     4321 tagtctgttt tctctggata taatgctgct tcaatagatt ttataccagg atttgatatt
     4381 ggtgtaggag gtaatccctt atttttataa ctattatatg gtgaatcaat ttttaaatca
     4441 tcatatgtta caattttctt tctctcttca aatatatatt gtattgtagc atctgattga
     4501 agaggcatac ctaccttaag tctattataa aacacacttg ctattatagg tctgtctttg
     4561 tcaagaacag cttctttctc aattattgat gccatttcca ttacttcttg aagagtcata
     4621 tttaattctt tttgtttctt tttaaacttg tccgtgtatt tactgtcaaa tacttttaac
     4681 atttcgctta aaatatcttc ttctgattgt ttctttgaat taaaataata agtttctgga
     4741 tacaagaacc cttctaaaga ggttattcca tcttccttca aatatggaaa tttatcataa
     4801 aattcttgag gtttttttat taaattttca aaagtagctt tatctcctaa atttttactt
     4861 actaacattg ctataatttc tttagatgta gctccctctg gaatagtcac ttttatacca
     4921 tcctgatata ttttacctga aactaaaaga cttattatat cattgtttga atacgattga
     4981 ttaagtaagt atgttcctga ttttatagac ggagctttat tgctaacctt taccaaaagt
     5041 ttaaataata gttcattctt tattaattta ttttcatata atatgtcaga tatttttcca
     5101 acagatgctc cacttggaac gtcaataatt acatcttttt tgttattttt atcataagga
     5161 cctatttgta taaacacaaa tatacctgct aagataatca aaataacaat tattaatact
     5221 gctattttca atctattttc tttaaagttc ataatataat ctactcctta tgtatattta
     5281 tccattttct attataaaac tagatacttt aatt
//
LOCUS       DAEPOG010000217         5286 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEPOG010000217 DAEPOG010000000
VERSION     DAEPOG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.217"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1791
                     /locus_tag="KR432_003364"
     CDS             <1..1791
                     /locus_tag="KR432_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH0641871.1"
                     /translation="NAKTNYKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSN
                     TGNWMVIHKESYISIRYKQKKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETT
                     AVFNLASSFGKITKNDDLKLDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQ
                     TLLSSNCIIKQVDSPYNKNKKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNK
                     ETNIKINREQKLNLGHLTLKDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILN
                     LRYSDNLDFEKSLVTVSINDVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTF
                     NLSIKNSNCVTRESNNPWAYVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVI
                     MPDYSGSQAMTWMFRLGVTLGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNS
                     VIKMLNNNLNIKFDKNYSNFISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSM
                     NEKNLYLGMDYLLNKSKVNDLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINK
                     TTKVFLMISFITIIVVMILSMLYIKKYKRR"
     gene            1828..2913
                     /locus_tag="KR432_003365"
     CDS             1828..2913
                     /locus_tag="KR432_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0641872.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            2967..4091
                     /gene="wecB"
                     /locus_tag="KR432_003366"
     CDS             2967..4091
                     /gene="wecB"
                     /locus_tag="KR432_003366"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH0641873.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            4169..5143
                     /locus_tag="KR432_003367"
     CDS             4169..5143
                     /locus_tag="KR432_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0641874.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         2231 a          505 c          888 g         1662 t
ORIGIN      
        1 aatgctaaga caaattataa aaatacatta gaagtattag ttccaaaaga ttatataatt
       61 caaggatata atgaaataaa aataaaaaca tacaaaacaa tatctgataa aatttgccaa
      121 gatgattcca atactgggaa ctggatggtt attcacaaag aatcatatat atcaattaga
      181 tataagcaga aaaaggtgga aaactcaata aatgaatatc cgtatccata tgcagaaatt
      241 gaaaataatc ataaattaga tacaactata gtagtaccag ataatatgac tagaggagag
      301 actacagcag tgtttaattt ggcatcatca tttggaaaaa taacaaaaaa tgatgattta
      361 aagttagatg ttaaattata ttcagaaatg aaaaattggt cagatgataa tattatatat
      421 attggtaaac ctgaaaatac agcagaagaa attttggata ttttaagtat aaaggaacaa
      481 accctattat cttcaaactg tataatcaag caagtagatt caccatacaa taagaataag
      541 aagatgatgg tggtaattgg tagtaatgaa gatgatttaa taaaggcatc aaatttatta
      601 atagaaaata gactttctaa ccaagtttta tcatcatcag tgcttgtaaa caaagagact
      661 aatataaaaa ttaataggga acaaaaatta aatttaggtc atttaacatt aaaagactta
      721 ggatattctg actttttgtt ggaaggagca tttaatcaac aagctttgtt tgatgtcaaa
      781 ataccaacgg gtaaggtatt agatgatggt tcaaagatta tcttaaattt aagatattct
      841 gacaatttag attttgaaaa atctttagtt acagtgtcta taaatgatgt tattgtggga
      901 agtaaaaaat tggataggtc acattctaat aatgataagt tagaattaaa aatacctaaa
      961 gatatagata ataaaaacta ttatcaagtt aaattgacat ttaatttgag tataaaaaat
     1021 tcgaattgtg ttactagaga gagtaataat ccttgggcat atgtatcaaa taattcttat
     1081 ttggcgttat ctacgaaaga aaatgaaacc ttatcttttg aaaattatcc gtatccattt
     1141 gttagagatg atgagtttaa tgatttaact gtgataatgc cagattattc agggtcacaa
     1201 gctatgacct ggatgtttag attaggtgtt actttaggta cgaatataaa ttcacataat
     1261 ggaaatataa atgtaattag aggaaaagaa tttagtgaca agtataagga tactaatatt
     1321 gtagtttttg gagtgccaca taataattct gttataaaga tgttaaataa taatttaaat
     1381 ataaagttcg ataaaaatta ctccaatttt atatcaaatg ataagataag ctttattgat
     1441 gattatggaa aaaatatttc taccattcaa ttaataaaat caccatataa taatcaaaaa
     1501 aatatcatgg taattagttc aatgaatgaa aaaaacttat atttaggaat ggattatttg
     1561 ttgaataaaa gtaaagttaa tgatttaaaa ggagatacat tgataattga tgaatacgga
     1621 gaggtagaag atttagctta taatttaaaa cctaagaaag aagtaaaaga ttcaagctgg
     1681 aatatgagta taaataagac aactaaagta tttttaatga tttcttttat tactataatt
     1741 gtagtcatga tattatcaat gctatatata aaaaaatata aaagaagata atatgataaa
     1801 tttattattt taaaagaggt atataatttg aggaaatata tgaataaatt taaattaaaa
     1861 agaagcattt taatctcatt aataatctta atttttatat ttatagtgta ctttgtaagt
     1921 attgagttaa aagatgggag tctagtaaat aatcaatata aaggtaagat taaatcggcc
     1981 aatttatctg tagattatga aataaatcaa gttatgaagg atatagataa attgggattg
     2041 aatacagtaa atgtacctat aattataaat gtagagtcca ttaattctga tattatgtca
     2101 atagataata atagtaaaga aaaggcaatt aagttaataa aaaaattaaa taaaaaaggt
     2161 atttcaacaa tattagaggc atatccatgg gttgaaaatg gaaaattgta tgagactgac
     2221 tggaacccta taaataaaaa aagatttttt tatacttggc aaaatgccat attaaatgaa
     2281 cttataattg atgtagcaaa tccactggat gtaaatgttt taaatattgg ttctaatttt
     2341 gtacatttgg aagaatatca acaaaattgg ggtgaaataa tagattttgt acaatccaaa
     2401 tttgatggtc ttgtaacgta cagaactaat tggtggtata caaaaaaaga tgattcaagt
     2461 agtaagttgt tttatgaaca aaaattgaac aataattttt ttgataagtt agattttata
     2521 tctatagcag catattttga gttaagtaat aaacctgtta atacagtgga tgaattaatt
     2581 tctgccttac actcatctac tgtaaacaat agaggacaaa atataaaaca agaaatatat
     2641 aacctttata aagctcatag aaaacctata ttttttggag aattaggttt ttcaaataga
     2701 gaaagtgcaa gttctcagcc gtggaatcat actccttcaa aagctgtaaa tggcgaagaa
     2761 caagcaaggt gttttgaggc atataagaaa gtttttgaaa atgaagactg gataaatggt
     2821 ttttctgtgt tttgtgtagg taaaatagat gacgaaaaaa atttttaccc atcaaaagag
     2881 agtataaaag ttattaagtc ttggtatgaa taattttaaa agtgtagaat ttatatagat
     2941 taaatagatg attaggagag atataaatga atgacatcaa ggttatgact gtatttggaa
     3001 ctagacctga agctatcaaa gtagcacctt taataaagga acttgaaaaa agagagaata
     3061 taaaaagtat tgtttgcgtt acagctcaac atagagaaat gcttgaccaa gttatagaaa
     3121 ccttcaatat aaatgtagac tatgatttgg atataatgga aaaaggacaa agcctgaatg
     3181 atataacatg taagatttta aataaacttc ccttgatttt aaacaaggaa aatccaaata
     3241 taatattggt acatggagat acaaccacaa ctctagctac aagtttgact gcattttata
     3301 ataaagctct tgttggtcat atagaagctg ggcttagaac ctatgataaa tattctcctt
     3361 ttccagagga gttaaataga caacttacag gtattattgc tgatatgcac tttgctccta
     3421 ctaatttggc tagaaaaaac ttgatttctg aaggaaaacc taataataat atatttgtaa
     3481 ctggtaatac tgctattgat gctttaaaga tgacaataaa agaaaactat aatcatccta
     3541 taattgatga gattggtaac gataggatga ttttgttaac ctctcacaga agagaaaatt
     3601 tgggtaaacc tatgaaaaat atattcagag ctataaagcg aatagtagat gattttgaag
     3661 atgtacagat agtatatccc atacatttaa atcctaaaat acgaactata gctgatgaga
     3721 tatttggaaa atttcctgaa aaaattcata ttatagagcc tttagatgta gctgattttc
     3781 ataacttttt aaataaatct tatatgataa tgactgatag tggaggaata caggaagaag
     3841 ctccatcatt aggtaagcca gtccttgtgt tgcgtgataa aacagaaaga acagaaggaa
     3901 ttgaagcaaa gacacttaaa ttagtaggta ctaatgaaga tagaatatat aattcagtga
     3961 gcgatttatt aataaataaa gatagctatg ttcagatgag taaagcatct aatccatatg
     4021 gagatggaaa tgcaagcaaa tatatagtgg atataataat taaaaaattt aattgcaaat
     4081 atttaaatta gataaggtct aaaacattaa ctaattgata taagatttat gataatatta
     4141 aggaatacaa tttttaaggg ggagtaatat ggctagaaaa ttaataaaaa atttaggtaa
     4201 aagtaaaagt gtaaagagag tgaaactttt atttaagaag atttttatta ctgtattcat
     4261 agtagcaagt atagttgcta tttttaatat aacgaaatat tttgaggaat tatacaaagt
     4321 aagggattta aaaagtacta aaattgaata ctatatggat gtggctgatg aagcaggaga
     4381 tggaaaggtc caattaagtt ggaaggcctt acttgctata gacatggtaa tacatgatga
     4441 agatttaagt aatataaaaa agaaagacac attggatata ggggaaaagt ttatagtaga
     4501 agataaaaat gataaaggcg aaaaggtgta taaggtaaaa aagtttaata aggtattaag
     4561 cgaattgaaa tttgactctt ctcaaaaaag tagagcaaga aaatacatga aagatttgga
     4621 atacacatac cttggaaata aacaattaga tagtagtgat gaaaaaatta aatttataaa
     4681 gaagttagaa gactcagcta taagagaata tattgattat ggaatattgc cctctgtaac
     4741 aattggacaa gctatattag aatctggctg gggaaattct aaacttacaa aacagagtaa
     4801 taatttattt ggtataaaag cagataaagc atggaaagga aaaagtgtag aaatttcaac
     4861 ttcagagcat tataatgaaa aaattgtagc tagttttagg tcgtataatt cattacaaga
     4921 ttctgtcaaa gaccacagtt tatttttaat taataataaa agatatagaa aacatgggtt
     4981 gtttgaggca aaagattata ttagtcaagc tcaagcatta gaaaatgcag gatatagtac
     5041 agcagaagat aaaaaaggaa atcgtatata tgcagagcta ttaatagata ttattaggag
     5101 ttataatttg caattgatag acaataaagt tgagacaaaa taattgttaa ctttatatga
     5161 ggagataaca agataagatt cttttctaca aaaagaatag aaaaattgta aaaaaaacta
     5221 aaaaaatgta ttgacttgtt atttaaaagg tgatattatt attactgtac tcgaaagaga
     5281 acaaaa
//
LOCUS       DAEPOG010000218         5175 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEPOG010000218 DAEPOG010000000
VERSION     DAEPOG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.218"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            58..756
                     /gene="yunB"
                     /locus_tag="KR432_003368"
     CDS             58..756
                     /gene="yunB"
                     /locus_tag="KR432_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBH0641875.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(968..1975)
                     /locus_tag="KR432_003369"
     CDS             complement(968..1975)
                     /locus_tag="KR432_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH0641876.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            2203..3087
                     /locus_tag="KR432_003370"
     CDS             2203..3087
                     /locus_tag="KR432_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0641877.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            3289..4839
                     /locus_tag="KR432_003371"
     CDS             3289..4839
                     /locus_tag="KR432_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0641878.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4868..>5175
                     /locus_tag="KR432_003372"
     CDS             4868..>5175
                     /locus_tag="KR432_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBH0641879.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     "
BASE COUNT         2049 a          587 c          837 g         1702 t
ORIGIN      
        1 acaaatatta taaaaattat gccaaagcta catataaata aataggaggg gataattttg
       61 aatagaatag tagatagaaa aacaaaaaat gaatttagaa aaatcatagc tattttttta
      121 gtaattcttt tcttgtctgt atttataggc agttttatat atatagataa aactttaaga
      181 ccgacaataa cagttttagc agagactaaa gctcttgaac tagcaaatag gtcgataaat
      241 aaagctgttg ctgagatggt agaaggaaaa ataaattatg aagatttaat ggatatacaa
      301 cttgataata atggtaaaat aactatgata caagcaaaca ctataatgat gaatgaaatt
      361 gcatctgcaa tagctttaga aatacaagat gaattaaaaa aggataagac agcaagttct
      421 tacataccta taggaacagc attaggaagt cctatattag ctaaatacgg tcctaaactt
      481 gaagtgtcta ttgaacctat aggaacagta tcagtaaact ttaaaacgga atttgagtct
      541 tcaggtataa atcaaactag acatagaata tatttagaag ctcaaacaca agttaaagta
      601 gtaataccac ttataacttc aacaaaacaa attaaagctc aaatacctat ttgtgaaact
      661 ataatagtag gtgatgttcc agaaagttat gtaaatattc cagaaaaaaa tctaggaaat
      721 gttttaccta acactggaaa gaataccaac aagtaatata aagttgtata tttttaaata
      781 aattcaaaat aatagtttat aaataaaatt tttggaaata atattaatat aattttaata
      841 aaaatttata ttttatacta tgtaaaatat ctaatatatt tggtctgtag gttaaataaa
      901 agtaccttta gaccaaatat attattaata acttaataac atcttaaaac ttatttattt
      961 atattattta caacatccat ttaataaata caccataacc tacagctggg tcattttcaa
     1021 ttgcatgtga cactgctccg attattttat tatcttgaac tattggagta ccactcatac
     1081 cttgtacaat accaccagtt tgagctaaaa gctttttatc tgtaatttta attttaaaac
     1141 ttttactttc tggatgtttt tggttttcta tatctataat ttcaatatta tattttttac
     1201 acttattaaa gttatcttgt aaaattattt gagcttttcc taatttaact tcatctaaag
     1261 atgctacttt atattttttt aaattggaaa tatcaaagtt attaatttga cctcttattc
     1321 caaaatcagt attttctgta aagctaccaa taatattttc tcgttttgca tttatacatc
     1381 caacactacc tttataagat ttttctatat taaggtgctt agttgtagaa attgaacctg
     1441 ttttaatagg tatttttctt gcattaccaa cacttattgg atgacctaca gctccaaact
     1501 ctgatgtatc tggattgata tacgttaaag tagcaaatcc agaaagtaaa ttgttaaaac
     1561 taattttttc taatatgtgt ttggtagttt tcattgttat aagttgtcca cctctattta
     1621 ataaaataga tacttgtttt tcttctggta aagcatttaa ggtattggca aagtcactat
     1681 atccatcaac ttcagaatta tttaccttta ctatagcatc acccaatcta aatggagatg
     1741 cttctccagg atttctaact atgatatttt ttaattctgc gtcaatttga agtacgtttc
     1801 caattggtat taaatattca ggttcatttt tttgtaaatt ttgtgcataa attaaattat
     1861 ttgagaaaaa atataataaa aataaaaatg ttaaaacaat tgtaaatttt gacttagcta
     1921 agttggtttt tttacttaaa gtattatttt taaaatgaaa atttttaagt tgcataaaac
     1981 acctctttct tattaaatta ttttaataat agaattgaag ttttcttgct gcttctatat
     2041 tatgttgtgc aagaattatg ttttgatgct tggaaaactg actcaataac accttagttt
     2101 tcataaaaat aaataaaata aatagtgtat atgataaaat aaatacatat tcatataaat
     2161 aaaaataaac aataactgtc tagcataaga aaggagcaaa aaatgaaaaa atatagattg
     2221 ttagttactt gtataattgc catgggaatc ataattatag gaacatcaaa tccagttagc
     2281 aatttttaca agactacaaa tttgattagt tcaggaaata tcacaaatga aaaagcagat
     2341 aaagatgaaa attcaaacaa aaataataaa gataaaaatg aagacaaatc taattctaaa
     2401 agtaataaag ataagtcatc agaggacaaa tcgtcagata aaaaaaataa aattcaaaaa
     2461 aataaaaaac ttttaatttg tatagaccca gggcatcaag gaaaaggaga tagcaattta
     2521 gaaccagtag ctccaggttc ttcctctaaa aaagcgagag tatcttcagg aacagagggt
     2581 attgctacaa aaaaaccaga atatgttcta aacttagaag catctcttgt tttaaagagt
     2641 atattagaat ctaaaggtta taatgtaata atgactagag agacacatga tgtaaatata
     2701 agtaattcag agagagcaat acttgctaat gataaaaaag cagatatggt tgtaagaata
     2761 catgcagata gtttaaataa ttcttcaaaa actggagcat ctatattgat tcctgaaaaa
     2821 gatggaaaat atacagctcc tatatatgaa gagagtaata aatgtgctga atttatcaaa
     2881 cagaatatgg aacaatcagg tatacaaatc aatggaatcg ttcaaagagg tgatttgact
     2941 ggatttaact ggtcaaaagt accagccgta ttagtagaaa tgggatttat gagtaactat
     3001 aatgaagacc aaatgatgtc aaatccagac taccaaagaa aaatgatgca gtgtatagct
     3061 gatggattgg atgcgtattt taaataagat atgaatagag aaaaagttag gttggtatta
     3121 ttttcctaat ttttatctct attttttatt gtacataatg caaagaagaa gtttatttta
     3181 ggttaaattg tcgtaaatat aactaaaaat attgggaata cttgatttaa gtttaaaaag
     3241 aatatattat agtatagaat gtgtaaaatt tttttgagag gtgttgaaat gctagtagta
     3301 gaaaatgtga gtcatggatt tggtgcgaga acaatattag aaaatgtatc ctttagactt
     3361 agaaaaggtg aacatattgc actagttggt gctaatggag aaggaaaatc gagtttttta
     3421 aatataataa caaagaagct tatgccagat gctggaaata taaaatggtc ttcaagagct
     3481 acagtaggtt acttagacca acatacggta ttaagcaagg aaaaaactat aagagaagtg
     3541 cttagagagg cttttaagca tatgtttgac ttagagcaag aaatgattgc tatgtatgac
     3601 aaaatgggag aagctagtga tgatgaaatg agtaagcttc ttgaagaaac agcagagata
     3661 caaactatac ttgaaaatag tggtttttat atgatagatg caaagataca agaagttgca
     3721 aatggtcttg gtcttggaga aattggtctt gataaagatg taactgactt aagtggaggg
     3781 caaagaacaa aagtacttct tactaaactt ctacttgaaa atccaactat tcttatattg
     3841 gatgagccta caaactattt agacgaagag catataacat ggcttactaa gtaccttcaa
     3901 gagtatgaga atagttttgt cttagtttct catgatattg aatttataaa taatacatgt
     3961 aatgtaatat atcatatgga aaatggagag ttaaatagat ataaaggaaa ttatgatgaa
     4021 tttgtaagac ttaatgatat aaagaaacgt caagaagaac aagcttatga taaacaagtt
     4081 gaagaaagaa aaagactaga ggattttgta gctagaaata aagctagagt agcaactaga
     4141 ggaatggcta acagtagaca aaagcaactt gataagatgg aaatattaga aagaccaaaa
     4201 gaaaaaataa aacctacttt tgcttttaaa gatgctagag cagctagtaa aataattttt
     4261 gaaacagaga atttagtttt agggtatgat gaagcattga caaaaccact taatttccat
     4321 ttagaaagag gaaaaaagat tgccttaaaa ggaatgaatg gtattgggaa atctactcta
     4381 ttaaaaactt tacttggaat aataaaacca tttgaaggaa atgttaaatt aggtgactat
     4441 ttggaagttg gatattttga acaagagagt tcaagggaaa atagtaatac acctatggat
     4501 gaagtttgga gtgaatttcc agggcttaca aactttgaag ttagacaggc tcttgcaaaa
     4561 tgtggactta caaatgaaca tataacgagt caaatgagag tattaagtgg tggagaagct
     4621 gctaaggtta gactttgcaa agtaatgtta aaaaatataa atttcttagt attagatgag
     4681 cctacaaatc atttagatgt tgaggcaaaa gatgaattga agaaggctat caaagagttt
     4741 aagggaacag ttcttttagt atgccatgag ccagaatttt attcagaaat tgttgatgat
     4801 gtatggaata tagaagattt taccactaaa atcgtgtagt gatttaactt attgctgggg
     4861 ggatttcatg aaaaagtttc taaatgtact tttttcagtt tgtattagta ttgttataat
     4921 agtaggtgtt attaatttta ctgttggatt taaacaactt tattattttg acattgatta
     4981 cttaaatata tcagagttat ctggtttatc tgaagatgat ataaaattga actacgatta
     5041 tctaattgat tataacttaa ataagaatgt gtctgagttc aagttaccaa cattaaaatc
     5101 ttctccacaa gggaagatac attttgaaga agtcaggaat atatttcaaa atataaataa
     5161 attagctaaa ttatt
//
LOCUS       DAEPOG010000219         5172 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEPOG010000219 DAEPOG010000000
VERSION     DAEPOG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.219"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            167..856
                     /locus_tag="KR432_003373"
     CDS             167..856
                     /locus_tag="KR432_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641880.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            856..1773
                     /locus_tag="KR432_003374"
     CDS             856..1773
                     /locus_tag="KR432_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641881.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1846..2772
                     /locus_tag="KR432_003375"
     CDS             1846..2772
                     /locus_tag="KR432_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0641882.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            2769..3449
                     /locus_tag="KR432_003376"
     CDS             2769..3449
                     /locus_tag="KR432_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641883.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            3894..4595
                     /locus_tag="KR432_003377"
     CDS             3894..4595
                     /locus_tag="KR432_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0641884.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         1987 a          551 c          804 g         1830 t
ORIGIN      
        1 tatactatat tttaattatt ttttcaataa aacttattga aaagaacttt aacttagaag
       61 agtatcacca aattgcagat acaatatcat aagcagttat ttattgacaa ccctttttaa
      121 taaatatatc atttgaagta tactgacgaa aaggagttga ataataatgg acatcttatt
      181 ggttgaagat gataagaata ttcgagaaat ggtatacgat tttctgatta gtgagaatta
      241 ttttgttaaa actgccgttg acggaaatac agctattgaa atatttaaaa attattcatt
      301 tgatattatt ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaaata
      361 tatacgaagt gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga
      421 taaaatgcta gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt
      481 agaattgctt gcaagaatta aagctaatat tagacgtgca acggtgtata gcagttcaag
      541 aaactctgaa gatatggtta ttcagtataa agatttgatt ataaacatat caaaatatgt
      601 agtttcaaag aatggaattg acttaaactt aacacataca gaatttgaaa ttgttaaatt
      661 actagcgact aatcaaggac gtgctttttc aaaggaacaa ttatacaaca ctatttggaa
      721 agaaccttat tatggaaatg aaaatgtgct aaatactcat attaacaggt taagaaataa
      781 attgtctgat ggtatagaaa aaaataatta tattaaaact ctttggggaa ttggctacaa
      841 aatggaggaa gaataatgga gttattttta gttgtaattt cgattgtact atttgctttc
      901 tacttaaaag aacgaaataa gtcaaagaaa atggaaagtg attataagta tatcaattca
      961 agacttcgtg atataacaaa tgatgatgaa attaattata ttttagtacc atctgatatt
     1021 tcgattgtaa aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagtagg
     1081 caaattaatt ataatagaag tgaaaaagcg atattgcaaa tatttacaaa catatcacac
     1141 gatttacgaa ctcccatcac agtattaaag ggttatattg aaatgctata tcttcaaagt
     1201 caaaaagaag aattatcttc ttcaatgaga gcaactttgg aaaaaatgca aacaaattca
     1261 aatgagcttg ttaattcaat aaataacctg tttaatatgg caaaaatcca atctggagat
     1321 atgatattaa atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag
     1381 ttttatgatt tactagataa agaaaacttt aatgttgaag ttaatattga agatagacct
     1441 atatatgcaa atgttgacac agaagcatta caacgtatac tcaaaaattt aattgataat
     1501 gctataaaat atggttcaga tggtagattt ttagggattt cactatatga aaaggaaaaa
     1561 catgttttta tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagcat
     1621 attttcacaa gagcttatac tgctgacaga aaaaaaggca atggtttagg tttggctatt
     1681 tctcaagggc ttgcaaactc tatgggaggt tcaatttatg taaatagtag acctaatgta
     1741 aaaacagttt ttacggtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa
     1801 agataatttt attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata
     1861 gagacccatc aattaaagaa aatatataaa gataaagctg ttgttaatga tgttaatatt
     1921 catgtgaaaa agggagaaat ttatggattt gttggaccaa atggagcagg taaaagtaca
     1981 gtaatgaaaa tgttgctaaa tctcgttaag ccaaattctg gagaaatagt tatgttcagt
     2041 aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat
     2101 ccttatttct atgagaattt gactgctaaa cagaatcttg atttacactg tgaatatatg
     2161 ggttattata acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa
     2221 tcaaacaaga aagtttcaaa atattcttta gggatgaaac aacgtcttgc aatagctaga
     2281 gcaattctta ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca
     2341 gaggggataa gagaaatgcg agaattattc cgtaagttaa atacagatta tggaataact
     2401 atatttattt ctagtcatat tctttcagag gtagaacaaa ttgcggacac tattggaatt
     2461 attcaagatg gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca
     2521 gattatgttg aagtagatgt agataatgtg gaacttgctg gatacttgtt agaaaaagaa
     2581 tttggaataa aagactttaa aattacttcg gaaagttgta ttgaaatata tgatttacga
     2641 aaagatgtaa aagaaatatc caaaatattt atcaaaaatc aaattggtat aaatagcatt
     2701 ggacgtaaac aaagttcttt agaagattac ttcttccaaa ctacaggtac aggaggtata
     2761 gaaacactat gatacattta atgaaactag aattaaaaaa agttgggcta aaaagatatt
     2821 ttttattttc aatactgggg attttgttat gtatgttttt tgtctttact ggattgaatg
     2881 ataaatctac ttcaatatat gattatgatg ttacatttag gacaatcagt ttagttttct
     2941 gtttttacta tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata
     3001 caaataaaac aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttat
     3061 caaagttact tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt
     3121 gttttacatt cgttgttgtg ttggataagt attttaattt agttattgga gattttcata
     3181 tttcaatttt atcttattgg ataccaggag caattaaaag tattattgta ttttgctcat
     3241 taggtatatg gacattcgtt gttggaatga taagaaaatc aatacctgtt acccttgtca
     3301 gttcaattat ttttatcaat ttaagacaat ttattttagc tggtacaaat acaactcaag
     3361 acagtctgat ttctgttttt attatagttg gaataactgt gattggtata tattatatac
     3421 taaatcataa agttactgaa attgactaga tactgtggat aggaaggtag ttattaattg
     3481 tatcttcttg ggataataat gatttgctat tatatcagct attattaata ttattagagt
     3541 tttaataata ttaataatag ctatatattt tttcttctta tttataaatt taatgattta
     3601 tagtcataaa atgaactcta agaagagata aaaatgcgtt ttatatgttt ttagattaat
     3661 atgaaatatc acaatgaaat gttgtacaaa gtgatatata ataattatat gaactataaa
     3721 attgactaaa cttattgact aataaattag atgaaaagtt gagacttact gaaaataaat
     3781 acaatgaaat atatactaca agtattaaat taaatctgaa ctttacatac tattagaata
     3841 agtattataa gattataatg atttaaaaat attaaaataa ttgaggtgat aacatgaaca
     3901 attccttttt agataaattg attgagacaa aagaacttaa aaattcactt tataatgtgt
     3961 taaaacacaa ttttctttat cattctaata agatagaggg cagtactttt actactgaag
     4021 cattagcact tttactcgat aagaatgttg ttacaggaag acatacttta gatgatgtac
     4081 aagaaactgt taattctagt tatgtatttg atacagtaat tgactcactg aaagagaaaa
     4141 taacacataa tttcttaagg aatctacatt caagtttaat cttcaataca accctacatt
     4201 ccagagggat ggctggtata tataaaacta tacccaatat gatactagga actgatgtaa
     4261 gcatagcaca accatttgaa gttgagccaa agcttgatga attaattgaa tggtactata
     4321 gtcagagtga agtgtctatt aaagttatag ctgaatttca ttatagattt gaattgatac
     4381 atccatttca agatggtaat ggtcgcatag gtagatttgt tatgctaaaa cagatgttag
     4441 aaaataatct tcctataaaa attgtatctt gggatagtga ggacttgtat agaaactcct
     4501 taaattcttg tagtttaggt aattatgtcc ctttaattga atatttatca agtttagaag
     4561 attttagaga agtatataaa atgttatgga aataattgtt tgtagcaaaa aaagttttta
     4621 tataaatata tttttaacta tgctatttaa cgttgatttc ttagttattc ttagcttgga
     4681 ctatacccac tattttaact atataaaata gtggatatag tttaactatc tctttataac
     4741 tatttttatg tatcttagtt gtttcttaat attgggacag ttcataaaac taaaagtgat
     4801 ttttaatttt atcatttata atttaccatt acatcagaaa aatgcttatc attagaattt
     4861 tcataaaagg tacctatttt cttcaattaa ctaaatcaat tctattagtc ttaatctttc
     4921 gtatttcaaa ttgataatac aagaaaataa aaaataaaaa attttaaatt ttttatttat
     4981 tgcgaaaaaa ataaaaaggt gctatacttt ttgtaacata ttattcaaat tattaagaga
     5041 tagtaaatat ttatagttta atttgtagta cactagcatg atttgcagaa caatgaatta
     5101 atctatagta taatagttta atttgaagta caacgattta atctatagta cactagcatg
     5161 atttgaagta ta
//
LOCUS       DAEPOG010000220         5127 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEPOG010000220 DAEPOG010000000
VERSION     DAEPOG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.220"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            8..910
                     /locus_tag="KR432_003378"
     CDS             8..910
                     /locus_tag="KR432_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0641885.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1519..2532
                     /locus_tag="KR432_003379"
     CDS             1519..2532
                     /locus_tag="KR432_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0641886.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2888..3457
                     /locus_tag="KR432_003380"
     CDS             2888..3457
                     /locus_tag="KR432_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0641887.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3794..4966)
                     /locus_tag="KR432_003381"
     CDS             complement(3794..4966)
                     /locus_tag="KR432_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0641888.1"
                     /translation="MIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT         1878 a          511 c          847 g         1891 t
ORIGIN      
        1 tggaaatgtg ttgccacaag attattatgg attaatagtg attttaggtg ttattcttgg
       61 agttatgggg gcattttaca actggtttac tctatatgtg cagtctttat acaagaagat
      121 tcctaaaatt gctacctttg gaaaactgtt gattccattt ataatggctg gtattcttgg
      181 gattgttatg cctgatgttc tggggagtgg acacaattta gttgaagaac ttacaagtca
      241 tgaatatatt tttggaatgg ttgttttgat ttttgttata cgttttatat tttcggctgt
      301 tagttttggg tcaggtgcac cagggggaat attttttcca ttgcttgttt taggagcatt
      361 tataggtgga atatttggta tggtaggtgt taaagttttt ggaatgaatc ccgattatgt
      421 aaataacttt gtgcttttgg ctatggcagg atattttaca gctattgtgc gtgccccttt
      481 gactggtatc atattaattt ttgaaatgac aggttctgtt agtcaaatgc tttcactatc
      541 agtaatttca attgtggcat atattgtggc tacacttatg aagtctaaac ctatttatga
      601 aagtcttctt gagaggttac ttgaaaaaaa tggagaacct gtacataaag agaggggcga
      661 aaagatacta gaccagttct ctgttatgtt tggctctcct gttgcgaaca aggcaattca
      721 ggatgtacag tggcctgaaa attgtctttt ggtagcaatt caaagaggtg gagaagaaat
      781 tattcctaga ggtaagacaa ttcttagacc aagtgatgta attgtgacta tgacagatga
      841 gagagatagt ggtgctgtgt atgataagat ggaaaatttg tgtaaagaac atacgtcaca
      901 tattttttag ggataatgat gaatgtttaa taatgataga atatttaatg aagatttgat
      961 taaaaaattg gtaatttgaa agagagttaa ggaattaata gaaaatgaaa atgtgatatc
     1021 ttagatgtaa aagagtttga agaaaataat tgtatatatt tgttaatgag attatatgga
     1081 ctaatggtat atttatactg tgctaaaagt tgcactattg tatgaggaat gggaataaag
     1141 ggtttgaaaa aataggtaga agatttttca gatattctta ttgggatatt ttaattaata
     1201 tattttaatt gcgatatttt gggaagaata gaatttattt ataaatgaaa ataattaaaa
     1261 ggggttaagg ttgtattagc cccttttgct tatagagttt ggaaatggta tcttgatagc
     1321 agttgtttta tgcttattat attttattac atattttaaa attaacctta tcactcctaa
     1381 atcattattc tatcaacttt taaaacactt tcaagacaat cacctattga aaacaaagtt
     1441 aattaaaaac ctacatatca gtttagtatt cgtgtagatg attatataaa tataaaagta
     1501 taagaaaggt gagtatctgt ggttagttca tttttaaatg atatctttag aacaatttta
     1561 gtaagttgta tttttatagt tattttatta gtctttagaa taactttatt taagactttc
     1621 agtaaaaaat ttaattacta tatatggctt attgtgatta ttaagctatc attaccattt
     1681 atgaattata cttttatatt taatgagtca aaatataatg gtaatattaa taagataagt
     1741 ttaggcaatt ttaatagtat ttctattgta tatagtactg ttttagtctc tgtttggatt
     1801 gctattacga tattttattt agtttatact ttattgaaat atattaagct taaaaatatg
     1861 ataaatgatt tgtcttatga tgttgaggat gataagataa aaaatcttta tgaaaattta
     1921 ttaaaagagc ttaagataaa taaaaaaata cggcttaaat attcatatga agttgaaagt
     1981 ccagtatttt ttaattcgtg tgtcatttta ccaccatgtg attatacatt aaaagagtta
     2041 aactggatat ttaggcatga gttgatgcat tttaagagta aagaccttta cattaagtat
     2101 ttggtgatat ttttaaaatg tgtttattgg tttaatcctt ttgtgtatat aatggagaag
     2161 tttatagatt tggattgtga attttattgt gatgaaagag ttttgaagaa ttgtacgata
     2221 gaagagaagc aggattatgc attgacaata attagtgcaa tgagaaaagg ttcaaatagt
     2281 tcaaataaat ttgtggctgg attacataag gagtcagata ttaaaaaaag agtgtttagt
     2341 atgtttaatg aaaagtacag aaatggtatt ttgatagcag ttattttatg tttattatct
     2401 tctattacat attttgaaat tgatttaatt actactaaat caatatttta tccactttca
     2461 aaacaaacac ctattgaaaa tagaactaat tcaaaaccta catatcagat taatgtttat
     2521 atagataatt aaaaaataca aagtttaaca agagattaat ataaataatt ttaataagta
     2581 tctttcacat atgttaaatt tactttaatt atattaaagc caatgaatta ttctttagaa
     2641 gctccaatgc cagtaaatac aggtaaagaa aaatctttta ctacaattgt aaattaaaaa
     2701 atattctaat atttaaagaa atgcaaaagt gtaagaaggg taaaaatctg ttataataca
     2761 ttaaaaacta aatttagaaa aatttacata attagaaaaa tgatttgata aaaaataaag
     2821 ttaaatatta actaagtaaa tttgaaaaag aaaaaatata aagtacaaat ttaaaaggag
     2881 gtaactaatg aaaaagaagt tggaagatat gactttagag gagttatgga acttgtttcc
     2941 tatatttttg actactcaca aagaatgttg gaaaaattgg tttaaagaag aacaatatag
     3001 actaactgga atattgccaa tgcaaaaaat tgttagaata agtcatattg gaagtacttc
     3061 tgtgagtacg atttatgcca agtccattgt tgacatactg attgagataa ttcctgatta
     3121 taatatgaaa gatattaaag atttgattga gtcgaatgga tatatttgta tgtctgagag
     3181 cagtagtagg ctttcactta ataaagggta tacagtagat gggtttgaag aacgcgtttt
     3241 tcatttgcat ataaggtatg taggtgataa tgatgagttg ttttttagag attacttaat
     3301 tgaccaccca gatgttgcaa aggagtatga aaaacttaag ttggaccttt ggaagaagta
     3361 tgagcataat cgtgatgctt atacagatag taaactagat tttgttaggg agtttactga
     3421 aaaggcaaag gtgttgtatt ctaatagata taattagaaa taggtacaat tagatattta
     3481 tcttatatat cgtattatat tttttaagaa agtaattaat agaaaaaggg gttgtttcaa
     3541 aatggagaga taaatccctt tttcattgtt gaaatgataa aaatatattt gatttcatgt
     3601 aaaaaagagt gcctcatcaa ctaaaaagtt tattttgaga cacacacatt tttattaagg
     3661 taaataaaaa tatcacattt gattaaattt taagttaatg aatatatgtg taaatattta
     3721 attataagtt attataattt tacatagtat aaaacaatct aatttgtatt atataagata
     3781 atctaatttt agactaaaat aatctaatat aaagaaatag agaaaattca ttctcagtat
     3841 aagagatatc caattttcca ttgtacttat taacaatttc tctaatattc tcaataccat
     3901 atccgtgaga attactatct tttttatctg aaataaatag ttcttgtttt ctattgatag
     3961 gatttacctt gttgtttgtc aataaatagc taaaatactc cttctcacaa cgtgccttta
     4021 ataccacttt tctatttgac aaatcaggaa ttttcaaaga tgcttctata gcgttatcta
     4081 aagaatttga aaaaatagta cataaatcta taggttctat acccaagtga ttgtctatat
     4141 ctatattaaa gtgcacatca acaccatttt cttccaaact tgtatgacga agatttaaaa
     4201 ttgagttaag agcttggttt ttgcaaaatt gtttgccact aagagtacct atacctattt
     4261 ttaactgatt aaaatacttt ttagctccag caatatcttc aatttctaaa tacgattcaa
     4321 tcatttgaaa gtgattattc atatcatgtc gcattttgcg tatttgaatt tgttgatttt
     4381 ctagtaagtt atagtatttt tcttttaggt gatttgcttg tttttctatc ttcatcttta
     4441 cattttcaat caatggctgt aaaagaagta tcacaccgat atttgccaga attgctgaca
     4501 agatgattag ataagtgcca taaggtaagt tgcctaagat aatattctta ttactaccca
     4561 tagggattaa attagtaggt ggcagaataa tagcaattaa tattgataaa aatgaagata
     4621 tacataccat atcaataaaa atccatattt tatttgataa gtattgtttt atattgacca
     4681 atctattctt taggaaaata tgtattaaaa accaaaataa taatttaatt agagcagaaa
     4741 ttatctggac tattgaaagt attaaaatat attcttttga gtgtataagt tcctcatata
     4801 tagggttgtt tataaaaaga aaattaagcc caattataat aggataaagt atcatgacaa
     4861 ctgatattat aggaattaat ttgcttttgc tacatattat aagtagtata aaatatccga
     4921 atagtgtaaa caaaatattt acggggtcgg aaagccatat tatcatttga ctaataatag
     4981 agtagcccaa aatcaatatg attttgaaaa tccaatgatt cctaaagtct ataaaatagg
     5041 aagtcaactt ataaagaaaa aaggctatga aaatattgat taatattaga ataaaattta
     5101 tgcttgtaga gattaaagaa taagtgt
//
LOCUS       DAEPOG010000221         5066 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEPOG010000221 DAEPOG010000000
VERSION     DAEPOG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.221"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            12..215
                     /locus_tag="KR432_003382"
     CDS             12..215
                     /locus_tag="KR432_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0641889.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            379..1533
                     /locus_tag="KR432_003383"
     CDS             379..1533
                     /locus_tag="KR432_003383"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH0641890.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1546..2550
                     /gene="rseP"
                     /locus_tag="KR432_003384"
     CDS             1546..2550
                     /gene="rseP"
                     /locus_tag="KR432_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBH0641891.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2733..3788
                     /gene="ispG"
                     /locus_tag="KR432_003385"
                     /gene_synonym="gcpE"
     CDS             2733..3788
                     /gene="ispG"
                     /locus_tag="KR432_003385"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH0641892.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4136..4960
                     /locus_tag="KR432_003386"
     CDS             4136..4960
                     /locus_tag="KR432_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641893.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         1966 a          521 c          985 g         1594 t
ORIGIN      
        1 gaggtgataa catgatttca tacgctccat tatggaaatt gttaattgac agaaaaataa
       61 agaaaatgga atttgtaaat atatcaggta taagtatttc tgttttaggc agactaggaa
      121 atgataaatc tgtttctatg gatacaatgg agaagatatg tttagcacta gactgtaaaa
      181 tagaagatgt tgtggaaata aaaaagggca tttaataata ctagaatatt ttataatatt
      241 ccacaagata caaaaaataa tagattaatt ttaatctgtg gaataatata tgaaatttct
      301 atgaatagta aaaataattg ttttagtagt gttataatgt tttgacaggg tatatttatt
      361 attagaaagg attattatat gaaaaagata tcaattttag gttctacagg ctctataggt
      421 aaacaaactt tggatgtggt tagagaacat agagataagt ttgagatagt tgcaatatca
      481 gctaacagta atattgaatt attgctagaa caaatagtag agtttaaacc taaatatgta
      541 acagtttttg aggaaaataa ggcattgaaa ttaaaagaaa tgttgcctaa aaatatagaa
      601 atagaagttt tagcaggaat ggaaggatta aaaataatat catcacttga tgaagtggat
      661 gtccttttaa ctgctgtagt aggtatgata ggattagtcc caactctttg tgctataaag
      721 aagggaatag acatagcatt agcgaataaa gaaacgttgg taactgctgg tgaacttgta
      781 atgaaagaag cagaaaaata caatgtaaat attcttcctg ttgatagtga acatagtgct
      841 atatttcaat gtctaaatgg agaaaataaa aaaaatatag aaaagataat acttacagca
      901 tctggtggtc catttagagg aaagaaaaag ggagaacttg taaatataac taaaaatgaa
      961 gctttgaagc atccaaattg gagtatggga agaaaaataa gtattgattc ttcaacgctt
     1021 atgaataaag gacttgaagt gatagaagct agatggttgt ttagggtaga acaagaaaat
     1081 atagatgtag tagttcaccc acaaagcata attcattcaa tggtgcagta tgcagatagc
     1141 tcaataattg ctcaattagg gtgtccagat atgagactac caatacagta tgctcttaca
     1201 tatccagata gaatggaaag tagctttgaa agaatgaatt tttcaaaatt tagtacttta
     1261 acttttgaag aaccagattt agaaactttt ccatgtttaa aattggccta tgagtgttta
     1321 aaaatgggag gaacttattc ttctgtttta aattcagcaa atgaagtttt agtaagtgaa
     1381 tttttagagg ataaaattgg tttttatgac ataccatatt acatagagaa gactttagaa
     1441 gttcatagta gcataagcga accaacatta gaagaaatct tagaaacaga tagatggagt
     1501 agagcttatg ttgcaaatct gataaaaaaa taggaaggtg aatttatgac tataatagcc
     1561 gcattaatac tatttagtat aattgtatta atacatgagt taggacattt tatatttgca
     1621 aaaagaagtg gtatcaaagt aaatgagttt tctataggta tggggcctaa gatatatagt
     1681 gtaaaaaaag atacagagta ttctataaga gcacttccaa ttggtggata tgtcagcatg
     1741 gaaggtgaag atgaagaaca aataagtcca aattcttttg gaaacaagtc tatattacaa
     1801 agatttagta ctattgtagc aggaccaata ttcaatataa tattagcagc aatactttta
     1861 gttccagtat ttttgtatat aggttctcca acgactaaat taggaaagat aatgccagat
     1921 actcctgcac aggctgtagg gcttcaagta ggagataaga taaataaaat taatggtaat
     1981 tctgttaaga cttgggatga agttgcaaat ataataaaca cttcttctgg tggagaatta
     2041 aaacttagta taactagaga tggaagtaat aaagttgtaa atgtaacacc taaaaataac
     2101 aatggaaagt atgaaatagg gattcaacca caaagagaaa aagacttttt ggcgtcaata
     2161 gtaaatgcat gtaaaactac tgtagatatg acaaaacaga tgttaacatt tctaggtcaa
     2221 atgattacag gacgtgtacc aggagggatt ggcaatgctg tagcaggacc agtaggtgtt
     2281 attggtatgg tatcagatgc tgctcgtaca ggcatcataa atgtagtgta tttagcagct
     2341 gtcataagct taaatttagg tatagtaaat ctattaccaa taccagcact tgatggatgg
     2401 agaatactta tgctattact agaagctgtt agaggtggta aaaaactcga cccaaacaaa
     2461 gaaggcatga taaatgttgt tggttttgga gctttaatgt tatttatgct ttttatcaca
     2521 tataaagata tattaagatt atttcaatga tttagttttt tagagatttt aagaaaatag
     2581 gctatgtagt tttaaataag aataaattaa tttttatgta tgaaatttat tcttatttaa
     2641 ctagatagct ttttaattta taatatacat gtaggttata atattttact atttaaagag
     2701 attcaatata aaataatata tagaggtgga atatgagtta cgagagaaga aagacgagag
     2761 aagtaagtgt aggaactgta aaaataggag gagaaaatcc tataagtata caatctatga
     2821 caaatactga tacaagagat gcagatgcta caatagctca aataaagaga ctagaggaag
     2881 ctggttgtga tatagttaga gttgctgttc ctgatattaa agctgctaag aatatagcaa
     2941 agataaagtc tagtgtaaat ataccaatta ttgcagatat acatttcgat tataagctag
     3001 cccttgaagc tatagaacaa ggtgtggatg gaataagaat aaatcctggt aatattggaa
     3061 gtagagagag agttaagatg gtagttgaga aatgtaagga aagaaattta aaaattagaa
     3121 ttggagtcaa tggaggttct ttagagaaag aacttttaaa aaagcatggc tctgcaactg
     3181 ctgaagcatt agttgaaagt gcaatgggac atattaagat tcttgaagac ttagattttc
     3241 ataatatagt tatatctcta aaatcatccg atatttataa aactgttgat gcatatgaat
     3301 taatatcaaa aaaagttgat tatcctcttc atattggaat tacagaatct ggaagtgttc
     3361 acaaaggaac aataaagtct tctatagggg taggagcact tcttcttaaa ggaattggag
     3421 atactgttag aatatcttta acaggcgacc ctgttgaaga ggttattgtt ggtaaacaga
     3481 ttttaagaag tttgggactt ttaaatgata agattaaagt tatatcatgc cctacatgtg
     3541 gtagatgtaa tatagactta attagtgttg taaatgaggt tgaagaaaag attggtagta
     3601 tggaaaaaaa tatcactgtt gcaattatgg gctgtgctgt aaatggacct ggtgaagcca
     3661 gagaagcaga tataggtatt gctgggggta aaggtgaagg tcttttattt aaaaagggtg
     3721 aaatagttag aatgattgat ggtaacaaat tagttgatga gttgctagaa gagattgaaa
     3781 aattatagta tacaattcat atatatatta attgactaga tttttaaata cttttgtaat
     3841 aagtttttat ttacagtatt taattatcta gtcatttttt attttataaa cttcattaaa
     3901 tgtaagaaac ttatattcta atttgactat atatgtatga gttaaatcat attgtaaatt
     3961 tataaaatta aaaaaagatt taatatagtg aaaatttcca tcaatagtat tgaaacttga
     4021 tagtaactat gataggatat agttactaga acagttggta aattatgtta acatagattt
     4081 taatgttttg gtaaattgtt ttattataag ttaatagaaa aagaggtgaa gaactgtgag
     4141 atataaaaaa atatctagct tacctatttt agttgttgct ttgctgatta ctggaacgac
     4201 aacaacattt tctcttagta cgaccaattc agaaaaaaca aatgtatact tagcaagtac
     4261 ggtaagtact gtcacaaaga aagtatccga tattcaaagt agtaaaggtc ttgtttataa
     4321 tgagaatatt ccattattta taaataatag caaaatacag tatgatgata ctccatatca
     4381 ttggcctagc aatgttataa gcttaacaaa tagcagtgaa aaggcaatta tggattatga
     4441 gattacatgc ttagcatatg ataaaaatgg aaaaccactt gagctatatt gggatgctca
     4501 aaatgtggca gcagatggcg aagtaggaag tgttggattt agcccagctg gtgtagacta
     4561 tggtattgtt acaggcatat ctcctgtgtc tccaaaatca tattcacata cttatagaaa
     4621 aatgcaacaa tcaccaccac aggatataat aagtatgttt gaaaaacaac aaggcaaggc
     4681 atgggtagaa aattggttga aagaatggaa acaaatggag aaagaatatg caaagcaaaa
     4741 tgcaatagca ccaggtaaga atcaaaatga tgcctttcta ttgtttgata aatggaaaca
     4801 atcaactgga gagcatggtg taaagtacat tatatcttgt gtaaaacaag taacatttaa
     4861 tgatggtagt atatggaaaa attcagcata tgagaattgg ttgaaaagtt ttcaagggaa
     4921 ggaagtttca aatagtgtat tagaaaatta ctataagtaa tgtttaatga tagcaaaatg
     4981 agtacgaatt taattaatgt taatttaatt aaataaggac tattttgttt aaaaatatca
     5041 gtgttacaag gaaattttta ttgtga
//
LOCUS       DAEPOG010000222         5062 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEPOG010000222 DAEPOG010000000
VERSION     DAEPOG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.222"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1235)
                     /locus_tag="KR432_003387"
     CDS             complement(<1..1235)
                     /locus_tag="KR432_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641894.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEV"
     gene            complement(1357..3900)
                     /locus_tag="KR432_003388"
     CDS             complement(1357..3900)
                     /locus_tag="KR432_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBH0641895.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(4178..4512)
                     /gene="rnpB"
                     /locus_tag="KR432_003389"
     ncRNA           complement(4178..4512)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KR432_003389"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4644..>5062
                     /gene="srtB"
                     /locus_tag="KR432_003390"
     CDS             4644..>5062
                     /gene="srtB"
                     /locus_tag="KR432_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH0641896.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNN"
BASE COUNT         1494 a          856 c          475 g         2237 t
ORIGIN      
        1 acttcaatag aattttcttt aagtagaggt tgctttgcat atataaatac atcttgcata
       61 agtttttgaa ccatattttt acttaagttt aaaagtactt tttgattatt aaattttatt
      121 tccgtttcta caaaattaga attttcgttt tcaagttctt tattaattac aatgtctttt
      181 atatcactat tttttataga tatgatttct ttttgaaaag actttagaat agtcaagcta
      241 tcgaaatctt cttgaataaa aaatggagca tgttttaatg tatatcccac tatttcattt
      301 tctatatctt ttaagtcatc tatttgcata atttctctat ttttatcatt tatatgtagg
      361 ttaaatacat ttttattatc atataatatt atatcttctc ctaaatttaa cttagaacaa
      421 ttatctttcg ctgactttac aatagtcttc ttagatttct tatttataaa aattaattgg
      481 tcttgatatt ttactaaaag catgtttgtg tttttataat caaatccatc tatatttcct
      541 gatatcttgc agaatactat atcacaatct ttaggaagat ttcctatttt ttggttatct
      601 ttaactctct catttaaatc agatggaact acactatcta tattgttatt aaatatagat
      661 acagacctta ctataatccc ctctctttcc atatgaaagt atcccttcaa tgacactctt
      721 ttatcttcta aaatgtatct ttctatatca tcaaaattta tttcaaatct atttttccca
      781 ctgataaata taaacttctc attatcttta ttaattccac ccatataaaa cttattgtca
      841 tctaaactta atatagtcat attatccttt agatagaata taaattctct ttgacttttg
      901 cagattaaat catattcatc caaagttatt ttcatagtca aatttgctgt aacattttta
      961 cttgaatttt cactttttat gtaaaaaggc aagttatttc catagacaat tatttgagaa
     1021 tcttctaaaa ctgcctctaa tttttcttta ttgtctgaaa aactatatat atattttaca
     1081 cctgtatcct cattaacttc atctgatttg ctgattatta tattccctag atttgttata
     1141 tgaagctttt tatattcttt tgagtatcta cttatgccac taagctcaaa cccatcagaa
     1201 agctcatata tgattttttc tcctaatttc aacatacctt caccacctaa acagaattat
     1261 aagtatttta taaaggtgaa taaaatgtct caaaacaaat aatatttttt taatactttt
     1321 tattctgaga catagtttct ccttttttat aatgctctaa gcaccttcaa ctatttcttc
     1381 ataaagttta tctaaatgtt taacatattc acgatatcca gatttgttct ttaactcact
     1441 tcttaaatct tcatattttt gtttaagttc ttcagcactc aaagaaggag ctgattcttt
     1501 taattcttct aacttataat catagtactt atcatacata gctatatatg tatcaataga
     1561 tttcttagct agttcattac atttttcttg tgcttctcca tattgagaat aatcacctat
     1621 ttttatttta tagtatccat tttcttgaat tattgttgaa gaaatacctt tagcctcata
     1681 ttcttttgct gtttcttttg ctgttttttc aactttaagt gaacctgcat aaacagtgta
     1741 atatacacct ttaccatctt tatatgcttc catttctttt gctttctttt cagcttgtgc
     1801 tttttcttga gcctcttttt cagcttttgc cttttcttct gcttcttttt tctctttttc
     1861 agcttgtgct tttttctctt cctcagttaa tttattgtta gcttgttgtt gagttgatga
     1921 ctttggaata ttctctttat catttttctt attgttgcct actgttataa aatatcctac
     1981 accaacaata accactataa ctactgctat aatagtttct tttaatctac tcttcttaga
     2041 aacaacctct tcatcatcta aagaatcttc tttatctgat acataatttt tcatttcttt
     2101 cttgttttct ctatatttgt ccttagaaga atttttagat tttttattac tattttcttt
     2161 ctttttaata ggtattacta ctgatttttc attttctaat ttttcttctt ttatttcttt
     2221 tgctttttta tcttctaagt ttttactttc tttttcaagc ttcgattcat tatttgaaga
     2281 tactttttca tctaagttat tatttattgg tttttctatt tttacactac gttcttcttc
     2341 tatatgttga cttggttctt cttgttttat ttcttttaat tcattttgct ctatttcttc
     2401 tgattttgct tgatgatttt caactggttt atctaaaatt atatcaggag aaatatcatt
     2461 taaattctct tcttcttgtt caacttcttc attgtctttt ttaataaatc caaataattt
     2521 agccttcttt ttactctttt tattttctgg ttcaattata tcttctttat tgctgtcgta
     2581 ctctttatct gtagtatctt tatcttctac aacgtcacct atatcttgat tattactatc
     2641 tacttcttct ttagcccctt cttcagcatc atcttctatt gaaatactac ttctaaagca
     2701 aatatcttta tctgcatttt ttatcatgta aatgtattct tcaatatcga tatctttatt
     2761 tcttctagtt ctttttgatg caatttcatg attttctaat tcactatagt tatcttttat
     2821 tagattctgt gctttattcc atgtatcttc actaatttct gttttatttt taattacata
     2881 gtccacaaat tccttaaaat tttcaggacc aaattctcca attatagtca gtctcattgc
     2941 accttctata aggtctctat ttcttctctt taaaaattct aatacaaccg ttgcacaagc
     3001 aaaatcttta ataatttctt tgccaaaacc atatccttca acagaatgaa gttcttctaa
     3061 tccacataac ttcttatatt cattgtgtat tgtttgagat atactttcat tatttaagct
     3121 atttaactta tctataaact tcgattctat atcatttgat tttgattcat gttttacttt
     3181 tgaattttta atttcattta gtagcatgtc attttctgaa ttatctttac ctaattgcaa
     3241 atcctcaatt tcagttgctt ttatttctac tttgtcttgt tcgttattct cttccacatt
     3301 attaggtacc acatctttat tgctagctaa tattttgttt acacagaata taatttctct
     3361 ttttaattct cttttcgcat ctgcttgatt tttaagaaat aaatctccac attctttctt
     3421 ttctaaactt atttttctaa gagatatatt aaggaggtct ttatataggt ttaaaacctt
     3481 ataatccttt tctaaacgtc ttcttctctc tgatagttcc attttatcat aagctaaaaa
     3541 actatttaaa tcattggctt ttagagagtc taatccctct ttcatagcct tatttacata
     3601 ctttctacat tttgctgtag ttaattctaa atctttttct aattcatcta tttcaggtat
     3661 gtacttatct actatccatt tgccaataag tggttctgta tttcttgcta atgtcttata
     3721 aaacaatata ttaaatttta aaagctctgc atattttgct tcattaaatt ctactttttc
     3781 aaatttcttt tccaaaacac catctagctt ttcatttatt ttagactctt gttttaaaaa
     3841 acttgctgtg acatttaaaa cagtgtttct tattatatta ttttcactca aaaccttcac
     3901 tccttaaatc aattatttta ttgtaatatt tacattgtgc aaagttgcta atttgattat
     3961 gatatctaaa taattattta ctaaatttgc tacatcttat atcatatcat attatttaca
     4021 tcattttact ttttcttttt atatctatct tatattatac catttttaat atctgtacta
     4081 acttatatta caatattcct caaaatttta aaagtagatt agacaaattt acaaataaaa
     4141 aaacatcagt ttaaactgat gtttttgact atcgaaataa ttaagcaagt ctataagcgg
     4201 agttctgtat tagataatca tctttctagg cctattgtca ccaataggct caagcgacct
     4261 acctaccgga gagtacgagc agcactcttt attcctatct tggtcttgct ccaggtgggg
     4321 tttacatagc caactagtcg cctagctgct ggtgagctct tacctcgcct ttccatcctt
     4381 accacaatag tggcggttta tttctgttgc actatcctta ggatcgctcc cactaggcgt
     4441 tacctagcac cctgctctat ggagctccga ctttcctcat gtaagattac atgcgattat
     4501 ctggcttact taatatttat tttacttagt tatgttaaca caaattcttt tacatgtcaa
     4561 agtaatatta tactaaaaat ttaaatttat tcaaaaatag tgtaatatag atatataaac
     4621 tccatttaac tcgaggtgat atattgaaaa aattatatag aatagtaatt aacattatac
     4681 tagtattagt aatactttac agtggattta atatatactc aaaacttacc aaatacaatc
     4741 atgatactaa aacatcctca gaacttcaaa aaaaagaata taaaaaagag gatttatcaa
     4801 agattaacag tgattttaaa ttttggcttt ctgttgagaa tacaaatata aattatccag
     4861 ttgtacaatc taaagataat tcttactatc ttgacaaaga tttttataaa aaagattcta
     4921 tttctggtac actgtttatg gattatagaa ataaatctat tgatgacaaa aatatcataa
     4981 tatatggaca taatatgaaa aataaaacta tgttcaacaa cctaaataag tttaaagatg
     5041 ctgacttctt taaaaacaat aa
//
LOCUS       DAEPOG010000223         4971 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEPOG010000223 DAEPOG010000000
VERSION     DAEPOG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.223"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(547..1188)
                     /locus_tag="KR432_003391"
     CDS             complement(547..1188)
                     /locus_tag="KR432_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBH0641897.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1431..2234)
                     /locus_tag="KR432_003392"
     CDS             complement(1431..2234)
                     /locus_tag="KR432_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0641898.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2390..3565)
                     /gene="thiI"
                     /locus_tag="KR432_003393"
     CDS             complement(2390..3565)
                     /gene="thiI"
                     /locus_tag="KR432_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH0641899.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3611..4762)
                     /locus_tag="KR432_003394"
     CDS             complement(3611..4762)
                     /locus_tag="KR432_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH0641900.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1557 a          934 c          467 g         2013 t
ORIGIN      
        1 tattgaaaat ttagatactt ttctaaaaat tatctatgtt aatagttaaa tttatgaaca
       61 ttttttgttt ttatgttagg atatttctta gaaatgaaat acttcttaat ttgatttgat
      121 atgtatattt ttatagctat tttattataa aaaaaaagaa cccttttaca actaagggtt
      181 actttttttc tctatttttt ttattatatc actatttttt ccctatttca atattttgac
      241 attttttcca ttatactgaa aagatatctt aaatttttaa cttggataaa ttttaaagca
      301 tatacttaaa attaacatac ttgttttcta ttttattaca cttaatcctt atttttatca
      361 ttctacaaaa caaaaataaa aaagaataag ataataaata aaatacttat caccttattc
      421 tttatttaat tattttacaa tgaaaaatct aaactacttt ttaaatagct tcctcttcca
      481 tcaatttatt tgtatattac tggtttaatt cttcaagtaa ctgattggtt cttgcaatac
      541 tttatcctaa actctggaaa ctttgtaaac tacctctcta gcatttggta aatttccatc
      601 tggaatacct acagcaatac caaatggtac agaataccct tctggtatat caagtagttc
      661 tctgtatttt ttatttgtat caaacttcat ctttattgac tctgattcct ttacatcttc
      721 tttatccaat acactcacaa gaccttcaat atccccttgt tcaacaggtg taaataaacc
      781 tctaaccatt cctattatac aacttccaag ccctagtgct tgtgcagctg ttgttatatt
      841 ccctgtaagt attcctatat catatggagc ccatgaacta ctttcatcat aagaaataaa
      901 taaaacggtt ggagcaccat aaaataactt aaagttttta tttatctcaa cattactcac
      961 ttcatgaatc ctagataaag tatcatcact aatctctttt aaaagtttct ggtcttgcac
     1021 aacagttatg tgccaaggct gtttattaca tcctgaaggt gctgcaaatg cacagtgtaa
     1081 taatgtgttt acttgctcat cactaatttg ctctgttgta aattttcgta tacttctacg
     1141 actttgtatt aaatttattg tttggttgtc ttgaaaatta ttattcatat ctattcccct
     1201 ttttatgtat ttcttagtac aaatactaaa tcttcagaaa attaatctta taattaaatt
     1261 tctttacaat attattataa ttatctttag cttgtatgtg aagacttaaa aatataaata
     1321 cttttaatat atcttaatta tacatataaa tgaaaatctg gtattcgtca tctatagaaa
     1381 ctgctctttt ctatagttta atcaatacca gattaataaa acatgatata ttaatttaaa
     1441 ggaccccatc cctctttaga atgaatctct gtttttacag acttaacact atctgtgtca
     1501 acatcccatg acctacctgc aacagaaata ccatttttac ctttatacca tctaccttca
     1561 tcataatcgt aaactaggga agtataccaa ttacctccag tcttattatt tgcaaataca
     1621 actacagtag catcagattt agtaggatta tatgcatatg gttgtatata tagtgtatcc
     1681 ttacttaatc catattttgc cttaaattca tctggaattg taggccaccc atttttatat
     1741 ttattgtcta ataaatattt tctaaaatca tcattgccct ttgaagctcc tggtattata
     1801 gaagggtctt gtgcaaatga tgcaatcaaa tctttcatac tttgatgtat atccctagcc
     1861 atttcaatac catctggatg ttttgtacaa gtaacataca cttttgcaat gctttctatt
     1921 ccagttattc catcataacc atcatcaaac gttgctgaat atattccacc tgacttacac
     1981 tttggttctg tttcaaagta cttaccatct ttgttttcta atacctcttt tatgacttca
     2041 ttcttgtctt tacttgattc ctcagccatt gcaataacaa tttgagtctt tatattttct
     2101 ctattagaaa gacatgtaac tgccttactt ttttctatat ttctaaataa cgctggaaca
     2161 gctactataa ctaaaattcc tattatagat ataactacca acaattcaat taatgtaaaa
     2221 ccctttttat tcataataat cattcccatt aaataaaatt atagtactta tttttaaata
     2281 ctatatatta ataaattgtc tacataaatc ctttcaacag aactactaat atacttacag
     2341 aactactaat atactcaata ttcaatttag taattaaata acttgtatgc taaaactcaa
     2401 tatcctctac ctctattttg tcaatagcat cctgtactaa tttttcaata tcaagtgctg
     2461 tttcagattt ttctattttc tctaatctag gtttagtgac aggctttcta ggagaaaata
     2521 cactacaaca atcttcttca ggcactatag atgtttcaaa agtccctatt tttttggcta
     2581 tatcaactat atctgattta tccattgcta taagtggtct aaacactggt aagtctacaa
     2641 cggcattagt acaagtaagc ccttgtatag tttgagatgc aacctgccca atactttcac
     2701 ctgtaattaa agcatcacaa tgtctttttt cagaaagtct ctgtgctatc ctcatcataa
     2761 atcttcttga aagaatagta gcttcttctt cattacaatt ctcaccaatt gctttttgta
     2821 tttctaatat gttaacttta tgaagtctta ctctaccaca gtattttgcc agtattttag
     2881 ctaaatcctt aactttttcc tgtgaccttt cacttgtaaa tggataacta tggaagtgaa
     2941 ctgcctctac ctccatacct ctttttgcaa ccatccatgt agctactgga ctatctatac
     3001 ctcctgataa aagagacata gctctaccat tcgttccaag tggcaatcca ccatatccag
     3061 gtatagtatc actatagacc atagtatgaa attctctgta ttcgcacttt attttaactt
     3121 ctggatttct tacatcaaca ttaatcctat ctcctacctt tgataataag tatccaccta
     3181 tatccatact catttcttga gaagtcaacc cgaaagactt atctcctctt cttgactcta
     3241 ccttaaaagt tttatatcca gcctctattt tttcttctaa catttttaaa gctatttctt
     3301 ttaatgtatc ataatctttt ttagctctta ctccagggca cacacccact atcccaaata
     3361 cttttctaac ttcttcaatt acttcttcat aatcatagtc ttctaaatca acatatattc
     3421 ttccatactc tttatataca ttaaatcttc caattggttt taacatattt cttatatttc
     3481 ttataagcct attctcaaat atatatctat tctttccttt tactccaatt tctccatact
     3541 ttactattaa tatattatac accatttcac ctcttaaact aatttattat cactatctat
     3601 acagtattct ttattttctt ttcattattg accttaagtc acaaattgat tctttcaaaa
     3661 ctttcacagc ttctaacatt tcttcttctg tattcatatc agataaacta aatcttattg
     3721 ttccatttat ttcttcattt gtaagaccta tagcatttaa tacgtaacta cctttctttt
     3781 tagaagaaca tgcagagcca gttgatacat aaataccttt ttgctctagg taatgaagta
     3841 aaacttctcc ttttgtccct ctaaatgata catttaatat atgacatact ccatcttctg
     3901 gagaattgat ttttatatct tctatattat caattatttc attttttaat atatttttta
     3961 agttatttac tttcgaaata acaacatcta aatctttgtt tagtattcta acagcttctc
     4021 caagaccata tattccaggt acattttctg ttcccgacct aattccaatt tcctgaccac
     4081 cacctgttaa cataggctta aggcgattat tttcttttat atacataaat ccaattccct
     4141 ttggaccatg aaatttatgt gcacttacac tcataaaatc tatattgtat ttagatggtc
     4201 taaaattaat ttttccatat gactgaatag catcaacatg gaaataaatt ttttctttta
     4261 aacccttcaa atactttcca acttcaccta ttggctgtat agttcctatt tcattgttaa
     4321 catgcatcat gcttactaag catgtagttg actttatcgc attttttaaa tcctctatat
     4381 ttattttccc atcttttccg acctctaaat aagttacttc aaaaccatct tcttctaaat
     4441 cttttaatgt atttaaaaca gaaggatgtt ctatgttagt agatattatg tgattttttc
     4501 tttttttatt taaactagta atacctctta ttatagtatt attacactct gtaccacctg
     4561 atgtaaaata aatctccttt tctttcgctc ctagagattt agctatatct tgtcttactt
     4621 cttttatagc tttttcaact tctacacctt ttctatggac agaagaagga tttccatatt
     4681 ccgtattaag tgcataaacc attttatcta taacttcttg ataaggtttt gtagttgcgc
     4741 tattgtctaa gtatatttcc aacttaatta cacctacctt tatttaaaat cttcaactct
     4801 aactattata ccactaatta tagtattttt tttgttaatc tttattaaaa agacaatctt
     4861 aaattataaa atattatttc atgaaaagct agttttttca ctttattaca aatttttata
     4921 gtaaaatata atatacttat actatacctt acaaaaatat attataaaat a
//
LOCUS       DAEPOG010000224         4933 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEPOG010000224 DAEPOG010000000
VERSION     DAEPOG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.224"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..179
                     /locus_tag="KR432_003395"
     CDS             <1..179
                     /locus_tag="KR432_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641901.1"
                     /translation="NMVIRENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTV
                     EAIFENKVSIIMEN"
     gene            359..961
                     /locus_tag="KR432_003396"
     CDS             359..961
                     /locus_tag="KR432_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBH0641902.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    1522..1760
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1824..3743
                     /gene="thrS"
                     /locus_tag="KR432_003397"
     CDS             1824..3743
                     /gene="thrS"
                     /locus_tag="KR432_003397"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH0641903.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            4511..>4933
                     /locus_tag="KR432_003398"
     CDS             4511..>4933
                     /locus_tag="KR432_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641904.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT         1953 a          488 c          877 g         1615 t
ORIGIN      
        1 taaatatggt aataagagaa aatttaaatt atgaggactt tttaataagt accttattaa
       61 ctctatgtcc caaaaaaata gaaatattgg atttgttaaa taataattgc tcaaaggaaa
      121 ttatagatac tgtagaagca atttttgaaa ataaagttag tataattatg gaaaactaga
      181 atctaagcat ataaaagcta gccatataaa attaaaaaaa ttctttttaa atcgacacaa
      241 aagacttata attgtatata aagctatagt ataactagag ttattaagta taaagaatat
      301 ctacttgatt aatgcaatat agctggcaaa tatacaattg ataaggagta attttataat
      361 ggataattta attaggatac tgatattagc tgttattggt ggatttatag gttatgttac
      421 gaatgtagtt gctataagac tgatatttag accaatagag cctattaaaa tacctatttt
      481 aaatatagaa atagtaggtc ttataccaaa gagaagagca gaaatagcag caaatgtagg
      541 agaaataatt caagaagaat ttctttctat ggacgaaata cttgcaaata tcattactga
      601 cgaagataag gaagaagttg taagatacat aaaagcaagg gtaaaaatta taatacatga
      661 gaaagtttca tttatcccga gtggtataaa aaacatgata caagattatc taggagagat
      721 aattgagtct gaagttaagc aaagtataga tgaattgagt aaaaatataa taaataaagc
      781 aaatgaacgt atagatatac aaaaaatggt cgaagataag attaatgaac tagatttata
      841 tgaattggaa gagataataa taagaatagc taagaaagaa ttaaaacata tagagttttt
      901 ggggcttgtc ttagggtttt taataggtat agctcaaggg ttaattacta tgtttattta
      961 aatatatgag aagtagcatt tatttaagta ctaattatat ttacctattt gagtgaatta
     1021 cttattaata gtatttagat attaattgta tttacctatt tgagtgaatt acttattaat
     1081 agtatttaga tattaattgt atttacctat ttgagtgaat tacttattaa tagcatttag
     1141 atattaattg tatttattta tttgattgtg ttacttatta atagtattta agtatttact
     1201 gtactactta ttaactatat taagtattga ttgtatttaa gtatacaaaa cctaaaaaga
     1261 gtacaaaaat tagaagttag ttttaataaa aattagtttt agtatttatc tagtcatatc
     1321 tatcatgcat gtggatacgt tttaaaaatt ttaaatttat gaaaatatta taatatcact
     1381 ataaatcaag agattaattt aatataaaaa tataagaata ttggtttaca gctgattgag
     1441 atgaaaaata atgggtaatt aatatattac attaatgttg attgatgtat aactatctgt
     1501 tataatgaaa atatattaaa aatcatagtg ttgaagagga agagtagagt agaaaccatt
     1561 ctcagagaat ttgggtctat ggctgagagc ccatgtaata ggtctatttg aaaactacct
     1621 tggagctgag cttattagca gtatttgtat ttgtactata cgtgaaagtg ctcgggtgac
     1681 gaccttatag tcatcaagag ggttgtatat acttgtatac gccaacttgg gtggaaccgc
     1741 gaattaatat cgtcccaaac atatttgggg cgatttttta ttacaaaaaa tctaaaaatt
     1801 atttcaataa ggaggaaaaa acaatgatta aagttgcttt aaaagatggc tcaataaaag
     1861 aatttgaaaa tgctatttct gttatggatg tagctaaatc cattagtgaa ggattagcta
     1921 gaaatgtggt agcagcgtct gtgaacggag aggttgtagg tctggaccac attatagata
     1981 ctgattgtga cttgaattta tttaagtttg aagataaaga aggtaaggaa gtttttagac
     2041 atacatcagc acatatatta gctcaagcta taaaaagact ttacccagaa gcaaagcttg
     2101 ctataggacc aagtatagaa aatggattct actatgatat agatttagac catagattag
     2161 ttccagagga cctagagaaa atagaagctg aaatgaaaaa aatagctaaa gaagatttaa
     2221 aaatagaaag atttgaactt cctagaaatg aagctttaga gcttatgaaa gaacaaggag
     2281 aagactataa ggtagaactt atatctgatt tacctgaaag tgaaataata tctttctaca
     2341 agcaaggtga ttttactgat ttatgtagag gaccacattt accatctact aaaaaggtaa
     2401 aagctgttaa attacaaagt gtagcaggtg catattggcg tggtgacgaa aataataaga
     2461 tgcttcaaag aatatatgga acttcttttg agaaaaacaa agacttagaa gagtacttac
     2521 atttactaga agaagctaaa aagagagacc acagaaaatt aggtaaagaa ttaggattat
     2581 ttatgatacc tgaagaaggt ccaggattcc ctatgttttt accaaaaggt atggaactta
     2641 aaaatgaatt attaaaattc tggagagaaa tacacagaaa agctggatat attgagatag
     2701 aatctccaat catattaaat agaaaattat gggagacttc aggacactgg tatcattaca
     2761 aagaaaatat gtatacagtt aaaatagatg atgaggatta tgctataaaa cctatgaact
     2821 gtcctggtgg attaatttac tataactctc aactacattc atatagagat ttcccaatga
     2881 gagttgcaga gcttggtaga gttcatagac atgagttatc tggtgcatta caaggattaa
     2941 tgagagttag agcatttact caagacgatt cacatatatt catgttacca gagcaaatta
     3001 aggacgaaat aaaaggagta gctaacttaa tagatggtat ctacaaaact tttggatttg
     3061 aatataattt agaattatct acaagaccag agaactctat gggaagcgac gaagagtggg
     3121 aagcagcaga aaatggatta agagaagctt tagaagagtt aggtctacca tatactatta
     3181 atgagggtga tggagcattc tatggtccta agatagactt ccatctaaaa gattgtttag
     3241 gaagaacttg gcaatgtggt actatccaat tagatatgca acttccaaga caatttgata
     3301 atacttatat tggtcaagat ggtgaaaagc atagaccagt tatgattcat agggttgctt
     3361 ttggtagtat agaaagattt ataggtatat taatagagca ttatgctggt aaattcccag
     3421 tttggttatc tccaactcaa gttaagatac ttcctatatc tgataaattt atggattatg
     3481 caaatgaagt taagaaagag ttatttgata agggtataag agttgaatta gatgatagag
     3541 ctgaaaaaat tggatttaag ataagagaag ctcagcttga aaaagtgcca tacatgttaa
     3601 tagtaggtga aaaagaagta gctgataata atgtttctgt tagatcaaga gataaaggtg
     3661 aaataggtag tattaaatta gatgaattta tagctagtat ttcaaaagaa attgaatcaa
     3721 gagaaagtat tattcaagac taatatataa tatattagta gctaagatat tgttatataa
     3781 gataagttta atcaatatgt tgatatgttg atacatagtg attaaattta tgaaaagatg
     3841 agtataatta gaggtacagt taatcatatt tttatgatta atgtacctct agttttttgt
     3901 atggtagatt tattaataaa tatatcatag aagtgttttg aacatggaaa tatttatgtg
     3961 cattctttaa ataaaagttt attttttgaa tgataattaa gtgcttgttg gtaaggttat
     4021 ggctgtaaaa atcataactt tatttttata tttgttccat tttcataaaa attattatcc
     4081 ctttaagaaa atttcttaat cctcttgaaa tatttccttt ttagtctaaa gttaaatttc
     4141 tttcttcaaa atgaatattt aaatgaacta aaatttaagt acattttcta atcttatgag
     4201 tctatttttc atagataatt gccctataat agaagaatct atgttggggc tatgtggtga
     4261 caataacagc attgtcagtg attaattaat gctttataaa tgattaatta gtgttttatg
     4321 ttatttttgg acttaccctc tatacataga ttaatttatt taactttaga tatagagaga
     4381 atgtcaagta catacacaga cattagagtt taagaaagtg tttatggagc aagaactttt
     4441 aaaagaagta gtgacttagt ttaatttcaa gaaatgataa atacaaaaga ggaaaaagag
     4501 aggtgttgaa atgatagaaa aagtagaaga aaaatctaag attattaaaa catcaaaaga
     4561 atataagcga actataattg aagaaaaaga taaagaatgg accaaaaaat taaatgatat
     4621 acttagtaga agtagttttt gtaatgatga attagataaa atcaaggcgt taattccaaa
     4681 agagattttg actaatgaaa atgttggaga agttgtgact gaagatggat atgctaagcc
     4741 cgagtatgat gaggtattta aatcattgtt cactttaaat aatgaatatg atttacttgt
     4801 aaattttata aatgatattc taaaagatgc tccatatgct aacaggaata tcaaaccgtt
     4861 cactcaaata aagagaataa ttagagttga aactgatcca acaattaatt acattggaga
     4921 gaaaagacct aga
//
LOCUS       DAEPOG010000225         4914 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEPOG010000225 DAEPOG010000000
VERSION     DAEPOG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.225"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(204..1388)
                     /locus_tag="KR432_003399"
     CDS             complement(204..1388)
                     /locus_tag="KR432_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0641905.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KR432_003400"
     CDS             1761..4112
                     /locus_tag="KR432_003400"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBH0641906.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KR432_003401"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KR432_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBH0641907.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1894 a          568 c          786 g         1666 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atat
//
LOCUS       DAEPOG010000226         4765 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEPOG010000226 DAEPOG010000000
VERSION     DAEPOG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.228"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(241..1041)
                     /locus_tag="KR432_003402"
     CDS             complement(241..1041)
                     /locus_tag="KR432_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0641908.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(1356..2087)
                     /locus_tag="KR432_003403"
     CDS             complement(1356..2087)
                     /locus_tag="KR432_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641909.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(2074..2769)
                     /locus_tag="KR432_003404"
     CDS             complement(2074..2769)
                     /locus_tag="KR432_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0641910.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(2771..3562)
                     /locus_tag="KR432_003405"
     CDS             complement(2771..3562)
                     /locus_tag="KR432_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0641911.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(3572..4384)
                     /gene="modA"
                     /locus_tag="KR432_003406"
     CDS             complement(3572..4384)
                     /gene="modA"
                     /locus_tag="KR432_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0641912.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         1648 a          656 c          585 g         1876 t
ORIGIN      
        1 ataacaaata tttattttaa tgtagtttaa tataaatagg tagtttaatg tagatatgta
       61 taaagtattt aataaggctt ataggtttta atgatgatat ctttatatat tttaagagat
      121 gatgaaatta gatatgggta atagttgaat gagaaaaagt agtatagcac aaaaagtgct
      181 atatcacaaa atctcaaata atttccttct tataacttaa attaaaagaa taaatatttc
      241 ttataaatta ggtgtatgaa gtatagcagg aacatcttta ttatttaata acttatcaaa
      301 tgatttatgg tcagacttat agccaatacc ttctctatat tctaattcat caacaatttt
      361 gtattgttga caggcatttt taaccttttc atagtcttca gtagaatgag ctttataaac
      421 tatcttatta ttaaaagtta ttttatcagc aaatttaggt tttacaaaat atttagcatc
      481 ttttatttta ccaacattat ataaattttg gataacagaa ccatttttta aatgttgtaa
      541 atggtcatct aacttataag tatgtgctat atgagttaca tttttaaatt tttcttcaaa
      601 tgtaagagca attttttcca tgtaattgaa gtcagtatta ccatttgtat ttgtttctgg
      661 taataatatt atattttccc ttattacacc aaggctatta agagaagcta aaaattctct
      721 atttctatat tctgattttt gtatatttgt taggttttca tattttttat atttatcaaa
      781 gacggatata ccacatccag tagatactaa aactacaaat acatgatttt ttcctctttc
      841 ttttatagca ttaacaatag cactcccagc ccacaacact tcatcatctt gatgctgaac
      901 atagaagaca acataatctt taaatttttt atgactataa ttcacatagc tactaaaacc
      961 ggttaaaatc aagcataaaa ctagaataat agatagaatt tcatatttat ttatagtaaa
     1021 ttttttattc ttaaatgtca tttaatcatc tcctgaaaag ttgtcttagt tatattatca
     1081 aacaaagtac taaaaattat ttcttaaatg ctgaaaaata tagagtagat taatggaata
     1141 aaattttttt tattagttta aaataaagaa aacatttatg tataagacta atcaaaatat
     1201 taataattga taggaattga gatttaaagg gattgtacat ttattcggga ttcattttaa
     1261 tagaatttta ctttagtact tagaataaaa gtaataactt tttaaattta aagagtacac
     1321 atatatattt gtgtactcct tgatgatatg attgcttatt tactacaaaa acagagtctt
     1381 tttaaaccta aaatatttga tgcaccagct atcgtagttc cataatagta aactggtttg
     1441 ttaagtgata gaaaatttat aattgagtta ttacataatg tacttcctgt gcaaatcact
     1501 aaatcagccc aagatattac atcttcataa tctcgtatac catcttcaat cttaacatta
     1561 tattgaatag tatccacaaa ttcaggattt aaatctaata ctcttgtttc gaaaaagtcc
     1621 ttaatattat caattatggc tggttgatat cctataattg ctacttttgg attaccaaat
     1681 tccattttta taaactcagg gaattttttt gcacatacct ctggctcgtt atttttacaa
     1741 tgaatggttc tatccgtctt gcctaagtat ttcataactg catttaatga tgctataaaa
     1801 agacttctat catgaggatt gtcatgtaaa tctaatgata atatgtcttt taaagaacct
     1861 tcaaatgtag atggagtaga agtaaatgct tgtcctttag ctcctttaaa gtcagcttca
     1921 agcattattt ctttaccaac tataattggg aaatctttac gcttagtatt tccaattgct
     1981 tcttttgaag atagagattt ggacacaata cttatcatat ccatatttaa attatattta
     2041 tcgacttgta ctaatagata atctctcaaa atatcaaata tattcataga aaaaagattc
     2101 tcctttattt tttatttctt catttaaatc aaaaatatga tttatttttc cattgttcat
     2161 tattcctatc ttatgagcaa aaaacattgc ttcagaaata tcatgagtaa catgaatcac
     2221 tgttggtttg tattttatgt agacatcctt caaaattttg cttatatgtt tttttgtaat
     2281 ataatccaat gaactcatag gttcatctaa aagtaatata tcaggttcag ctattaaggc
     2341 acgtgcaaaa gcaactcgtt gcttttcgcc tccacttaaa tttttaggat acctattttt
     2401 tatatcttct atctgcatta atttagacaa gtggttagcc ttaagaatct gttcatctat
     2461 aggtattttt cttaatttaa gtggaaacct tatattatct tcaacattca tatttggaaa
     2521 taattcaaaa ttttgatata caaatcctat atttctattt tctggaggta agtttgatat
     2581 taaattttct ttgtatgata catttccttt agcagatgga tatcttcctg ctaatgtttc
     2641 taggaataga gttttacctg acccactagc tcctaataaa acaaagtatt catgattatt
     2701 aatatttaaa ctattttctt gtaatttaaa attaggtaaa tcaagagata aattttttat
     2761 ttttaacata ttaattctta tcaacctctc tattaaaaaa tatctcaaaa agcatcaagc
     2821 atgtaagtga gataaatatc aaaattacag ctattccgat tgcaatgtct atatcaccag
     2881 tagacatatt taaatatata gatgtaggaa taatttcagt tttcatacgt gtagaacctg
     2941 ctatcatagc tactgcacca aattcaccaa gtgctcttga ccaagtcatt acagttgcag
     3001 atatgattcc agtttttaga tttggaagaa ttataaatct aaatgcttcc caactattac
     3061 atccaagggt acgtgcaaca aatatcatct tttcattact ttcatttagt gaagtgtgaa
     3121 gtattttaat agtaagtgga agatttacaa atacttgtgc taaaactata ccttgctttg
     3181 taaatataaa atcaagctta aaaatcttat aaatggaatc tccaatcccc attcttccaa
     3241 acaaaagtaa aagagcaatt cctgatacta agtgtggaag agacataggg agatttatta
     3301 tctgtataat cagtttttta aatggtaatt tagtaatatg taaaaaatat gctactggaa
     3361 tagctaataa aaggcatact atagtagata ttaaagatgt tttaatacta agtgttattg
     3421 catactgtac ttccacggat tttaagcata taggaacata acttatccct ttaatgatta
     3481 acataagaag tggtgataca ataaaaaaaa agactatagc aaaaaatcca gtaagtagtg
     3541 tagtaaaaaa tttatttttc aagtttaatc actacttaac aggttcaaaa ccatattttt
     3601 taaatatctg tttagcttca tctgactcta tataatttat aaatttatta gctaaatctt
     3661 ttttatcact atagtttacg attcctgctg atatgagttg gtctatattt ttatcttggg
     3721 caatttctat aacttctaca tcttcattgc caaagaccga atcttttgtg gctattgtag
     3781 catcagcttt acttgatgtt aactgtacaa tcatttcatt tacagttgca gctgtagaga
     3841 caacattatc atttatagat tttaaattat ttttttcaag tattttttgt gtagtcttac
     3901 ctatagcatt agactctttg tctcccaaaa taagtttaac tccatcttta tttaagtctt
     3961 ccaatttttg aatattagct ggatttcctt taggtacaac tattgcaggt gtatgatgtg
     4021 caaccgtttt atgttcacct acaagttctt tttcaaaggc ctttgaataa attggttcag
     4081 aaccaacaat aaatacgtct cctttgtgac ttgtctcaat ttgagctatt agctgtgcag
     4141 aacctgcata gctatattca actttgacat tattttcttt ctcaaatttt tttgctattt
     4201 catccattgg tttctttaaa cctgcccctg aatagacaaa aagagtctct ttttcttttg
     4261 aatcatcagc tgttgtcact ttagtattag attcatcatt agctttgttt tctttagaat
     4321 tacatgcagt aatacttaat gaaataatca tagctaaagc tagtactgaa agttttttaa
     4381 acataagata ttccccctta gtaaattagt gtaaattctt ttttagtata tcatactaag
     4441 aaaacgatgt ctaagttttt gttatgtttt ataatgtttt gttatgtttt attatgtata
     4501 gttaagaaaa aatctattta taatgtcatt tccataggta gttattttat ttttaatttt
     4561 tattaatact ttaaagtatt ttaaatttaa aaacttaaat caaattaact tattgtagat
     4621 tttttacttt ttaactaatt ttatatttat tatatttcag tgaaaaaata gaacattgaa
     4681 acatatagat atgttttaat tgctttttat taacaaatta ctaaaagtgt tatatcagaa
     4741 ttgaatacaa aagaaagagt atgta
//
LOCUS       DAEPOG010000227         4711 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEPOG010000227 DAEPOG010000000
VERSION     DAEPOG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.229"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            361..1227
                     /locus_tag="KR432_003407"
     CDS             361..1227
                     /locus_tag="KR432_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0641913.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            1340..1744
                     /locus_tag="KR432_003408"
     CDS             1340..1744
                     /locus_tag="KR432_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBH0641914.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            1922..2371
                     /locus_tag="KR432_003409"
     CDS             1922..2371
                     /locus_tag="KR432_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641915.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2527..2904)
                     /locus_tag="KR432_003410"
     CDS             complement(2527..2904)
                     /locus_tag="KR432_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0641916.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            3166..4083
                     /locus_tag="KR432_003411"
     CDS             3166..4083
                     /locus_tag="KR432_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBH0641917.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            4566..>4711
                     /locus_tag="KR432_003412"
     CDS             4566..>4711
                     /locus_tag="KR432_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH0641918.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT         1866 a          493 c          757 g         1595 t
ORIGIN      
        1 gtgaatttaa atgctatgtt atttaggttt gtatagttaa tttatatatt tctgatttta
       61 taaattgtat ttattattat atcttgattc taaaatcatt aaaaatttca acttctataa
      121 agatttacaa tttatcaaat acattatgag tatataaaca atttattatt acagtgctac
      181 atttaaaata agtcttagta taaaggtatt gttacaagta taaagaaact tatgtatgct
      241 attggaataa ataatacctt aaattataat taagaataag aactgttaaa taagggactt
      301 gattatcatt tagttataga taatataata ttaaaaataa agcttaagag gagaaaaaat
      361 atgatagata tacataaaaa tatatatgat aataagttat ttgaggagct aaagattgat
      421 tgtaagaagt gttttggact ttgttgtgta gctctttatt tttctgcttc agatggattt
      481 cctatcgata aggaatcagg aaaaccctgt attaatttac aaccagattt taaatgttct
      541 gtacataata gtcttatgaa gcgaggtttt aaagggtgta ctgcatatga ttgttttggt
      601 tcaggtcaaa aagtagcaca agtaacatat aaggggatag actggatgca atcaccagaa
      661 ttaacaaatc aaatgtctga agtgttttta attatgagac aattacatga aatgttatgg
      721 tatcttaaag aagcatctgt attaaatata tcagatacaa tcaaatcaaa aattgatttg
      781 ataattgaag aaactgaaaa gataactaat atgagcccag aacagataat taatttggat
      841 atcatatcac atagaacaaa ggttaattta ttattatcac aagcgagtga gtctgtgatg
      901 ggaaaggtta aatcatttat caagacttct actcttaaaa atatgaaaaa attaagtaaa
      961 aatatagatt taataggagc agaccttaga aaaataaatc taataggagc agatttaaga
     1021 ggtagatttc taatagcagc aaatttaaga agtacagatt tgagtggggc aaatttaata
     1081 ggagcagact taagagattg tgatattaga ggtgctaatc ttgaaaatag tatattttta
     1141 acacaattgc aagtaaatac tgcaaaagga gattctagta cgaagttacc agcttcacta
     1201 atacgcccta aatactggga gaaataatta aattaataca taattttaca aaatatgaac
     1261 ttttttgact ttaattcata ttatacatta gagattcttt acaagaattt cttaatataa
     1321 tatagggaga gatataaaaa tgagttatca aaattataaa tgtgaaagag attatgtgta
     1381 tgatgattat aaatacaata agtgcaacaa gtgctataat gattatgagg aaatgacaca
     1441 tgttcatgaa tattcagaaa gtgttaagtt agcagaagaa tgtgaggata gacataacca
     1501 ccgtgcagca ggggttactg gtgaagctat accaataaat ggaggtacaa atcatgttca
     1561 taaaataaat gataatgtag atttccttga ccatttccat aagatatgtg ttacaacagg
     1621 gccagcaatt agaatacctg gaactgataa acacattcac ttaatatgtg gagaaactac
     1681 tgttaatgat ggtcattgtc ataaattcct ttttactact caaatagaag cacctttagt
     1741 ataaactgat atggattatt gtagtaaatt tgctagatta tagtatataa taaatgttaa
     1801 aaatatataa tagataatat ttaaacttaa ttaggactat gatacttaac cttcgtagtc
     1861 ctttctaagt tttgtataaa aaagtgttat acaattaaaa gtgatatagg gaggaaattt
     1921 tatgaaatct ggaataattc ttgaaatgaa acaaataaat tcaacagaat atgacgaaat
     1981 gttgcaagtc tgggaatcat cagtaagagc aacgcatgat tttttaacag aaaaagatat
     2041 tgaatctttg ataccattag ttgaaattgg acttaaagaa gtagaaaata tagtttgtat
     2101 aaaagataat gatataataa agggctttat aggaatagat aaagataaaa ttgagatgct
     2161 ctttatagaa gataaatata gaggaaatgg tattggtaaa aaattaataa aatatgctat
     2221 agataaatac aatgtaaaat atgttgatgt aaatgagcag aataaaaaag ctgtaggatt
     2281 ttatattcac ttaggattta aagtgtttga taggtctgaa atagatggac aaggtaaccc
     2341 atttccaata ctacatatga aattaattta aagttatact tctcacttat tcgcaaaaat
     2401 atttacttaa atttttatac aaataaaagt cgtgacagtc ttaggacacg acttttttac
     2461 tacactatta aattgaaatt tatctgttct atattatgat tattagatgt agttttttta
     2521 tttttactat gtacttaaat accaagtata cccataccta tcaagtactt cagcagcgcc
     2581 atcattttga tgtggtggtg ggtgtggtaa aaattcttgt acaagtgtac caccctcggc
     2641 aagttttaca gatatttctt ctatcgcttc tcgagatggt agaacaacac aaatcgccat
     2701 gccagaaatt acaacaggct tgtctgtatc cgcacccatt atacaacctt cgtattttgt
     2761 gaaagtaaca cttgcgtgca taataggatt gtttggattg ctgtctaatc gcgcaaatat
     2821 agtttttcct cctccgaaag cctcagcata aaaattaaat gctttttcac aagtaccatt
     2881 aaatattaaa tatggagtta gcatgaccaa tatatcctcc ttgatataga ataatttata
     2941 gttgcacctg ataatctaaa ttatgattta gtaaattgta tcatagggaa accttgaagt
     3001 atataatttt ttattattat attatacaaa tttattaatt taaaaaaatg ttatatgtac
     3061 ggaaggttta caggataaaa tataataaaa agattaaggt gacattataa aaaatgtata
     3121 ttgacattga cgctacgtaa ttgtatatac tttgtcttaa aggagatggt aaatatgaat
     3181 attatagaaa ttcgctcaga taaaatatac aagaagataa tggatgcacc aataaacaaa
     3241 aaagaagata tatacagata tgaattgatg aagccttttg aatttaagtg gaagtgtatg
     3301 aatgttccaa tagttgctag acagaaaggt ggatatgatg taattatagc aagtgaaatg
     3361 ttaggggttt tatcgcctaa ggatattgat gaaaagcaaa aaaagaatat aaatgtgtta
     3421 tctgctgata aaatttgggg cacttgcaaa gaaaccatag aaaactctat aaatgctttt
     3481 ataaaagaag ggtatgattt aaacattaag gactataaat attcaatatt attggcgaat
     3541 ccaaatagtc cttatacaat attaagtgat ggatactggg gtgatggtgg gattcctgga
     3601 tatatatttc tatcattggt tcctaatgaa tatactatca atagattacc agtattaata
     3661 gcacatgaat gtaatcacaa tattagattt cagtttatag agtggaataa taatataaca
     3721 ttagaagaaa tgatgataaa tgaaggtctt gcagaaaatt ttgcaacatg gatgtttgga
     3781 gaggaaatgt taggaccttg ggtcagtaga acagatatcg aaacattaaa tacttatata
     3841 aagccaataa taaaaagtgc tttaaaagaa actggatttc aaaatataac atcttatctt
     3901 tatggtgatg atatagctaa aatgcaagga tattttccag tagggttgcc ttattgtgca
     3961 ggatatgctt gtggatatta tatgattaag tattatttag aaaagacaaa taaatcaata
     4021 atcgaagcga ctttattgcc ttatagtgag ataatcgaaa cagtaaaaga gttttgggaa
     4081 taaattttat aaattgcata atttgaaata aattatgcaa ttattttatt tttaagaaaa
     4141 aataaattga caataattta taagaaatat acaataatat atataaagaa atatttcaat
     4201 atttaaaagt tttaagattt catatctaga gtacctaaca ttacatgtta gtttcttgtt
     4261 acttatctaa ttactttata tttcgtagtt atatataaaa ttactataaa aaatgaaata
     4321 tattaaacaa tcaactaaaa aatgaaatat taataaaaaa aattcaaatt ataaatatta
     4381 aatgaatata attaaaacaa atatataaat ttaagtataa cactcacatt ttaatgtatt
     4441 gtgaaatttt tgttgaactt tatgtaccat aacttataaa atttatgtca tgttatattt
     4501 gaataagtaa taaaattgta gatgcaaatt aatctacaaa atttatatga aatggagttg
     4561 atgctatgac agaattaatg attataagtg ggttagtact attgatttgt ataacttcta
     4621 gcaaggtatt gtataaattt ggagtaccaa tattattgat ttttatctta ctagggatgt
     4681 tatttgggtc tgatggaatg gtaggaatct a
//
LOCUS       DAEPOG010000228         4682 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEPOG010000228 DAEPOG010000000
VERSION     DAEPOG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.230"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            88..465
                     /locus_tag="KR432_003413"
     CDS             88..465
                     /locus_tag="KR432_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0641919.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            458..1492
                     /locus_tag="KR432_003414"
     CDS             458..1492
                     /locus_tag="KR432_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBH0641920.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            1971..2144
                     /locus_tag="KR432_003415"
     CDS             1971..2144
                     /locus_tag="KR432_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0641921.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(2229..3251)
                     /locus_tag="KR432_003416"
     CDS             complement(2229..3251)
                     /locus_tag="KR432_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0641922.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3582..3764)
                     /locus_tag="KR432_003417"
     CDS             complement(3582..3764)
                     /locus_tag="KR432_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641923.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4077..4496
                     /locus_tag="KR432_003418"
     CDS             4077..4496
                     /locus_tag="KR432_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBH0641924.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
BASE COUNT         1823 a          570 c          663 g         1626 t
ORIGIN      
        1 aaaaatccac attttttcat aaagcgtttg actaaaaaat tcacatattg tagacttaaa
       61 gtagcaagag ttttgaggga ggtgtaaatg atgataagaa aaataccaca agcagaacta
      121 aaagtaatga aatttatttg gaaagtagat gctacagtga catcaaaaga tgttattgaa
      181 gttatggaac aaaaatatgg ttggaaacaa accactacac taacactttt atcgaggtta
      241 gtaaaaagag gattcttaaa tgctcaaaaa atagacagat atacacatta cacagtaata
      301 gtaaaacaca aagattattt aagttttgag acaaaggatt tcctaagtaa tatacatgat
      361 gattctttga aaagcttaat ttcagcatta catgatgatg aaaatatagg taaagacacg
      421 ctagattttt tagaaagtta ttttgataat ttaaagaatg aataattata tgctatatgt
      481 ttttgacaat cttataaaga caactattgt atgtagtttt ggtatttgtc tattattatt
      541 tttgaaaaga tattttttta agaggtttag taagagattt aattattata tttggttggt
      601 aatagtcttt agaatgttac tttttctatt taattataca gtaatgtatg aagtaaaaga
      661 gcccaaggaa aatacagtag gaaataatat tacacaaatt gatgtttcta tagataatga
      721 ttttatgctg tatttagttt atttatggtt atttgtaact atagccattg cagtttatac
      781 atttatggaa tacacaaaat ttaaaaatct cgttgttgat gtatcttatg atattgaaga
      841 tcatgatgta aattgtatct ataaaaattt gttaaaagaa ttagacataa aaaagaaaat
      901 tgagttaaga ggttctgatg aattgataag tccagcaggt atgggattat ttaaatctta
      961 catattctta ccagactata cttatagtaa agatgagtta acttggattt tgaaacatga
     1021 attaatgcat tttaaaaaca aagatatttt aataaagttt ttagttttgt ctgttaaaat
     1081 aatgtattgg tttaatccac ttgtttatat aatgagcaat aaagtaaatc tagattgtga
     1141 attatgttgt gatgaaagtg ttttgactga ttgctcttta aaagataaga aagcatatgc
     1201 gttagcactt ataaagtcaa ttaaacttag taagaattat aatagtggta ttttaacaac
     1261 tgaatttaat aagacaaatt tggaaaagag acttgatagt attattaaga aaaaagggaa
     1321 aagtggaata ttgatgttat tgattttgtt tacattattt tcagttacat attttaattt
     1381 tgaaagtatt agttttgata attcaaatta tttaagaaat atagggattt tagaaaaaac
     1441 aggcgcagat cttaaggaag aagatgatat tagcatgaga gttgtaaatt aatagatttt
     1501 tatagtatta taatttatta acaaattagg agaagttttg aaattaaaaa tctaaaaaaa
     1561 atcttagtag tcacagggag ggtgactcta atatctatta gtttagtatt tgcaaatact
     1621 aataatgatg taaatgtatg aaaaagtaat gaagagcaaa caacaaattc atttacaata
     1681 actggttacg aatttattta tgaaaatgta ccacaaccta taaaatctga ttatgaaaaa
     1741 aatgtaaaga attaaatata aatccatcta aagatgatac aatctttatt aatgaaaaat
     1801 ttatagattc atataatatt aatatagatg attataatat tgaaccaaaa gcatttatta
     1861 ttagatataa aaaagattta aatcgttttg atgctactgg tcctataagt tatagcgtaa
     1921 atataaatac cactgtagta ggatatggac atgtagaaaa agggaatcct gtgcatttag
     1981 tacaactttt atgtcaacaa ataaatggtt ctgatattga tccagatagt aaatttggac
     2041 ctgctacata taatcaagta aaaatacttc aaggaagact tggtgtaaca aaagatggtg
     2101 ttgttggaaa agccacttgg caagctgtcg gagagcgtta ttagaaaaga ggttgtcgca
     2161 ctaagaaata gggcgacaac ttttttatat agaatatatt aatcagtatc ttagagtcta
     2221 gaaatatttt acaataaatt ccatataact ctaccatcta tatccttaac attttgacaa
     2281 ccagtcaaaa tcatagtctc acataactca ttcctaacct tctctataaa aaactccaca
     2341 ccatcaagac caccgccaaa agaagcagta acaaaaggtc ttcccatcaa aacagcatca
     2401 gcacccaatc caagcatctt aactacatca acaccagtcc taactccacc atcaacaagt
     2461 attgttatct ttccctttac agacttagct atatctggaa gtacctcaca agtaccagga
     2521 gtataatcaa gtactcttcc accatgattt gaaacaataa tggcactagc accagactct
     2581 acagccatca aagcatcatc tacagtcata attcctttta taataaaagg taatttagta
     2641 gattcaacca attctctaat ctcatcaatt gtcttaggag aaaaaggatt ttcttgaaat
     2701 tgattattaa taagtccaca agcatcaagg tctacaccaa ctgcaaaagc acctgcttct
     2761 tcagacaatc taattttttc tattatcttt gaattattcc aaggctttat aaatacaatt
     2821 ccatttccac tattatcttt caacacatct aaattatcca gtaaaaaagt atctacatta
     2881 gtatcgccaa ccatagcata aattccagaa ttagaacaac ctctaacaac aggctctatg
     2941 tattcttttt cggaaacttt accacccatg ttcaatagag tacctgatac aggtgcagcg
     3001 aatataggag aactcatttt tctaccaaat aattctatgg atgtatctgg ctttgaaaca
     3061 ttatgtataa ctctcatatt tatctttatt ttttctagac tttttctatt ttcaatgaaa
     3121 gacgaaccac ttcctttacc acccatacca ggaacatctc cagcacaagc taatccatta
     3181 caaattttac atactttaca tgttccatta aagttctctc tagctgattt tagtaagtca
     3241 ttgtaattca tacaaaatct ctccttttta taatattaaa attgagtatt actatattga
     3301 aaatatctta attgtaaata tagggatgtt attatgatga gctaaactag ataatacata
     3361 ttagatattt tttattgttg tagatagtta aattatatca tattagatag tcaaattttg
     3421 aaaaaaaact aacttattta agtatacatt ttgaaaaaat taaaaaagct atatatagat
     3481 ttttaaagaa atctatatat agcttttaag tttatgtcaa tcttttttta ttaatgaata
     3541 aataatatag aatacaatat acaaaaaagt ttcttaaaat gctaagataa tccccattta
     3601 caaagcaaca tcattatact gtgtaaattt gaatcaacaa tgttttcacc atatttcacc
     3661 ataaaatctt ctaattgtgt gtcaataaat gaataatatc cacttatact atcgatgtat
     3721 gcagtcatta agtgtataca ttcatcactt ttatctttat acatatatat ctccccctat
     3781 atacaatata taaatttaga tactaaatcg tatcatatta aatagttaag tgtgtaacat
     3841 ctatgcagtt atatatttat atccttatat taataactaa agtgagtttt gacaatagat
     3901 tttgggaaac tggatgttca aagtgtaaac ttggatttta aaatcattaa ttaagatagt
     3961 gtataggaat atatgtatac ataacttaat tataaagatt ataaacaatt acttttatta
     4021 actttattta agtggtagaa taacttggag attaataatt ataaggagga tataatatga
     4081 gtttatacaa gtttatatat gatgataagg aatatttatt aaaagaggag aactgtagtg
     4141 ctttaataaa tgatgaagaa aatccagttc aaggagtaag tatttcaaaa ataattgata
     4201 tacttaatga agcagaagaa gttgattttg atgtagaata ttatcaagaa gcatgtccat
     4261 tatgtttaga aggagtaaaa gaaaagaaaa aattctttcc ttttttagaa tatcactttt
     4321 atattttcag taaagatgga gaatatgtaa taagcaatat atctgttgat tataaaggac
     4381 tatcttttaa taaactatca agggcaaata aagttgataa tagttatatt gtaagtgtga
     4441 taatttgtga aaattgtcag gattatatag ttcagataga aaattgtata gtttagtgta
     4501 aagtatgtct taatttaaga gaaatttcta aattgagaca tcaatttttt tcaaaaatag
     4561 tatggaaaat tactaaattt atgagataat gtaagtcgga ggtgagttac aatatatagt
     4621 aaatatttgg aagagataga tgatacaatt ttagattcaa tgtttaaact aaccttgaat
     4681 ga
//
LOCUS       DAEPOG010000229         4637 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEPOG010000229 DAEPOG010000000
VERSION     DAEPOG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.231"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            332..1633
                     /locus_tag="KR432_003419"
     CDS             332..1633
                     /locus_tag="KR432_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0641925.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1709..1984
                     /locus_tag="KR432_003420"
     CDS             1709..1984
                     /locus_tag="KR432_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0641926.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="KR432_003421"
     CDS             3309..3539
                     /locus_tag="KR432_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0641927.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="KR432_003422"
     CDS             3532..4113
                     /locus_tag="KR432_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0641928.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
BASE COUNT         1806 a          377 c          903 g         1551 t
ORIGIN      
        1 aagtaagata ttactatcag gtagtgaatt atattgaacg atatatcaat aattggcagt
       61 aagttatatc aaataagata ttactatcga gtaatgaatt atattgaaca atgtattaat
      121 aattggtagt aagttatatc aaataagata ttgctattat gtagtaaatt atatgtatca
      181 gaataaatag tatattaata atttgtagta tatcatgacg aataatatag tcataacaaa
      241 tagtgagtca taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata
      301 aaaacttata aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga
      361 gaagtctatt ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt
      421 aaatgaaaac atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact
      481 taaaaaagaa aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc
      541 tctagcaata aaaacgagtg atgtgactaa tctaattact tggggtcaga attttggtct
      601 tgatggtcat attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg
      661 ccaaaacctt ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt
      721 tgaaagtaat ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag
      781 tatagcaggg aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg
      841 tataaaatta cttgatacta ctataggagg tctttttaaa ggagagctaa ccacaatagc
      901 agctaaatcg ggggttggga aaactgcttt ggcacttcaa ataatgttaa attcatttaa
      961 gcaaggtaaa aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag
     1021 aaatatatgc agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga
     1081 aaatgattgg aaacttattg taaatgctat aggtgattta agcgaaaata acttaattta
     1141 tattaatgat aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc
     1201 agatttatta atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag
     1261 agaaagggag gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat
     1321 acctgtaatc caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga
     1381 tagacctatg agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca
     1441 tcaattaaag gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc
     1501 agttagagca tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag
     1561 agatggacag actaggcata agcatttttg ttactttggg gataagttac attttcaaga
     1621 actaaattat taggtcaata aagtagtaat aacaagctta ttaaaagtca ggaaatagca
     1681 aatagattat aaaatatgag gtgaaaatat gttggaatta acgaaaatta tagaattact
     1741 tgaagaacac aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga
     1801 ataccttgaa ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg
     1861 ggcattaaaa gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat
     1921 ggaattagcg aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa
     1981 atagatattt ttggagaata caagtgtgat atacttctga aatagaagtt attatagttg
     2041 aaataaaatt gtggctaatt tgtgtataag tttaagatta tagaggagta taagtgtgat
     2101 taacagcatg attttgtgaa taaaattgat gatatatatg agttaaatgt gtgattaata
     2161 aggtaaggtt gtgtataaga ttatgagtat ttaaaaggag gggctataat taatagtgta
     2221 gagttgtatg tttgcttaag attatataat aatttaatag attaaactat acgaataagg
     2281 taaaatcgtg tgtttagtta aagcttatta taaaatcttg atatgcgatt aatgatgtaa
     2341 tatgtgaata aagttaagat tataatagat ttaacctgtg gctaatagaa taaaattgtg
     2401 aataaagtta agattataat aggtttaacc tgtgattaat agagtaagag tgtaaataga
     2461 gataaacttc aatgtgggtt taacttgtgg ttaatagagt aaggtgtaac taaagataaa
     2521 cttcaatatg ggtttaactt gtgattaata gagtaaggtg caactaaaga taaacttcaa
     2581 tatgagctta acctatgatt gatagagtaa gaatgtaaat agagataaac ttcaatatgg
     2641 gcttaacttg tggttaatag agtaagagtg taaatagaga taaacttcaa tatgggttta
     2701 acttgtggtt aatagagtaa gaatgtaaat agagataaac ttcaatatga gcttaacttg
     2761 tgattaatag agtaagagtg taaatagaga taaacttcaa tatgagttta acttgtggtt
     2821 aatagagtaa gagtgtaaat agagataaac ttcaatatga gcttaacttg tgattaatag
     2881 agtaagagtg taaatagaga taaacttcaa tatgggctta acttgtgatt aatagagtaa
     2941 gagtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgtata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttggt attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta taggaagaga ttttgtgaag atttaagggg
     4141 tttaatgttt aaagctagag ttgatttaag aaaataagaa agatataaat atctaaatat
     4201 gaaatagatt caagatgagt aatagtttct attagctatg ataggtatat gagtggagca
     4261 taaatgaagt ttgtataaag taaactcatt tatgcttctt ttttacatga tacattacta
     4321 attaaataga aaaacaatgt aacaacttgt attttaagta cgtcagagaa atctgaagaa
     4381 atgataaatg ttatagaaga ttgtaaactt atgcttaaaa caagtaatag atatttaaag
     4441 gaagcagatt aaatttcaag aagtcttacg tgcttgttga aacataaact ggatttgtta
     4501 cagataaact aatttataca atatttttta attaatatgt tttcattact atatattata
     4561 tgttaataca taaaaaaatg gtggctgttt tattaaaggt aggtcaatga catttcttat
     4621 aaaaataaat tctaact
//
LOCUS       DAEPOG010000230         4598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEPOG010000230 DAEPOG010000000
VERSION     DAEPOG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.232"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            291..938
                     /locus_tag="KR432_003423"
     CDS             291..938
                     /locus_tag="KR432_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641929.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            931..2325
                     /locus_tag="KR432_003424"
     CDS             931..2325
                     /locus_tag="KR432_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0641930.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            2750..>4598
                     /locus_tag="KR432_003425"
     CDS             2750..>4598
                     /locus_tag="KR432_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0641931.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT         2003 a          439 c          790 g         1366 t
ORIGIN      
        1 aaagtatctc agaaagattt ttaatcagaa tgaggtgctt tttcatttgc atataaaata
       61 ttaagtaatt tcaaaaaaat ataaatttat ttaatttaat taaaatatta taatatatta
      121 gttacatatt aaaatatata atcgagatat tgttaagaca ttgtcgagat attataatat
      181 ataactaatg tattataaat agttgtaaac ttcctgtaac atatatttta tataattaaa
      241 gtaatggata taatatgaaa tataataatg aattttaggg atgtgaataa atgaacatat
      301 taattgctga tgatgaaatt agtatgctta agatactttg tgcatatttt aaaaaagaaa
      361 acttcaatgt gtttactgca aaaaatgggg aagaggcttt agatattttt tatgaaaata
      421 agatagactt agctatatta gactggatga tgccaataat tgatggaata gaggtttgta
      481 aagagataaa ggaaaatagt aatacaaggg tacttatgtt gactgccaaa agtcaaagtg
      541 aagatgaaat agaagcactc aacattggag cagatgaata tataaaaaaa ccatttgatc
      601 ctagagtttt aataataaga gctaagaaac taataaatta taaaaataca attaatatta
      661 aagatttaaa aatagatttt gggtcaagta aagtttttaa aggtgataag gaattattat
      721 tgactaagaa agaattagaa ttaattcgtt gtttaatcaa taataaagga atggttttat
      781 ctagggaaaa acttttagat ttagtatggg gattggatta tgagggagat tttagaacag
      841 tagatactca tataagaaga ttaagggtaa aaataggaga agatataata aaaacttata
      901 gaggattagg gtatagtctg gaggatttta atgactaaac taaacaaaaa attgtccata
      961 agtatatcta tagtagttgt agttgtttat ataatatcta taattataaa tagtatgttt
     1021 attcatagat attatttaca tgaaaaaaga aatatattgg ataatgttga aaatgaaatt
     1081 aaagataaag acatagataa attaaaatca gacattactt taattgaaga aaaaaataat
     1141 atagatatcg tatatataga attagataaa aatcataaag ataaaggtaa tatagataaa
     1201 ataaatcaag atttattaaa tgcattttgg aaaaaaggtt taagtttaaa taagttttgg
     1261 attgaagaaa atagtttaaa taatattgat aagaaaagta taaataagat atacaatcaa
     1321 ggtaaaacaa aatatagttt gcttgtaaaa tttatcaaga aggataatta tttgtttgct
     1381 atatctatac ctatagaaca ttctgaagaa actattggca ttgtaaatag atttaatata
     1441 ataatgggaa ttttttcagt agttataata actatactta catttatatt atcaaacaga
     1501 gttataaaac ctatggaaag gttaaagttt ttatctaaag atatatctga attaaatttt
     1561 aggactgaag atataaaaac gaacgatgaa atagaagagc tagcttacag cataaacata
     1621 atgagcgaaa aacttgaaaa agcacataat gaattgaata aaagaaatga gaatctaaaa
     1681 acttttatat cagatgcatc tcatgaaatg aaaactccaa tagcacttat aaaagcatat
     1741 gcaataggta tgaaagatgg tttggatgat gggacttata cagatacaat aattgaacaa
     1801 gcagaaaata tgaccaatac aataaattct cttttatatt gggcaaaata tgagaagaag
     1861 gagagaagtc tgtgtctggt agatttaaag gaaaggttat ataaaagttt aaaaaactat
     1921 gagctgttga tagaaagagg tagtataagt gttaaatgca gtgtagatga taaagaactt
     1981 ataataagtt cggatgaaga tagtatagat atggtattta ataatttgat tagtaatgct
     2041 ataaagtata ctaataataa tgaaatagag attaatcttt ttgaagagaa tgagagaatt
     2101 atattatcca taaaaaatgg tatagattac aatatgggag atgatataga aaatatgtgg
     2161 aagccttttt atgttttaga gaagtcgaga agtaaggagc tttctggtac tggtcttggt
     2221 cttaccatag ttagaactat acttgaagaa aataatttta gatacagggt tgaaatttgc
     2281 gatggaatta tagaatttta tattatcttt ataaaagtaa tttgaattaa aatgtaaata
     2341 taaagaataa ttaaaaaaat atcgcaatta tttgtttttc atatggataa aattggttat
     2401 ttaatatata attacaagta taaataataa aatttatttg taaaaagagt agcactttta
     2461 attctaaagg ctactttttt tatacaaaac catgtttttt ataacaattc attaacataa
     2521 aaatcaaact taccttaaaa tataaattta caaatttttc aatcaataag aaaatttctc
     2581 tattccttac ataatgactg taatgtaaca aaataattac aaattaatta tgaaatcatt
     2641 atagttagaa acctctttaa aattagatat taagtatcct ttagaattag aacgggaaca
     2701 aagttaaaaa attaagtaat atcaaatgga caagggggaa gaaaataaaa tgaaaatacc
     2761 aaagaagatt gctgcaatgc ttacagttac aatgatagct ggaagttcaa cagcaggcat
     2821 agctagtgca caaactgtgg caacaaattt aacagggcaa gaaagatatg aaactgctgt
     2881 taaaataagt caagatggat ggaaaaatgc tgatgaggta gtaatcgtaa acgactcatc
     2941 tatagcagat gctctatctg caacaccatt tgcaaaagca aaaaatgctc caatactttt
     3001 aacaagtaaa gataaattaa atgataagac gaaagcagaa atacaaagat taaaagctaa
     3061 aaaagtatac ttaataggtg gaacttctgt attaagtaca aatatagaaa aagaaataaa
     3121 agatttaaaa atatcttttg agagaatctc tggagctgag agatatcaaa catccttaga
     3181 attagcaaag aaattagatg ctataagtga tgttaagaaa atagctgtag ttaatggtga
     3241 aaaaggactt gcagatgctg taagtgtagg agcaccagct gcacaaaaca atatgccagt
     3301 aatacttgct gattctaaaa atggaacagc agttgcagac aaatttataa aagatgctgg
     3361 aataactcaa tcttatgtag taggtggaga gagttcaatt tctgaagctg ttaaaaataa
     3421 actaccaaat agtacaagac taggtggaac agatagaaat gatacaaatg caaaagtaat
     3481 aaaagaattt tataaaaaga cagatttaaa aaatgcttat gtaactaaag atggtatgaa
     3541 taaacaagac caacttatag atgcactagc agtaggtgta ttaggtgcta aaaatcaatc
     3601 accagtagtt ttagttggaa agaacttatc agcttcacaa aaatctctag ttaactctaa
     3661 gagttttgat aagataacta aagttggtgg gaatggtaat gagacagcat ttaatgaaat
     3721 gaagtcatta caagaagtaa aaacagttga agctaaaaca atttctgaat taaaatcagc
     3781 tatagataaa gctactgcta atgatgttat aaactttaag ccaactagtg aggttaaaga
     3841 agcatttaca atacaaactg ataaggcgat aacagtaaac ttaaatggta catatacaaa
     3901 gactgtgact atcaacatgc ctaatggtga tgtaaataac tatgcaaaag tggatgatgt
     3961 agttatagat gatgtaaaag atggaacttt tgttaactac ggaaaaataa ctaacttaaa
     4021 ggttaatgat aaaaatggag ccaagataga aaataattct aaaggtgaga ttggtagttt
     4081 aacagtagca tctggagcaa gtcaagtaaa agtgactaat ggtggtaaga taactacagt
     4141 tacaaataat tctaaaggaa ctactataga taataaaggt acaataagta gtgttaaggg
     4201 agataattct ccaacaataa gtggaaatag tccaagttct aactcttcag gaggaagttc
     4261 atcaagtgga ggttcttcac atggaggagg aagttcttca tcaaaagtag ataaagtagt
     4321 tttgaaaaat acaataacag cagcaaataa attgtataat gaagcaatag agggaacaaa
     4381 tgtaggtgaa tataaagtag ggtcaaaagc aatatataaa acagcaatag acaaagcaca
     4441 ggcaatatta gacaaatctg gtgtaacaca aaaagaagta aatgatgcag taacagcatt
     4501 aaacacagca acagacacat ttaaagcagg aaaagtagta gcagtagata aaacagcatt
     4561 acaagatgca gtaacagcag caacagcatt acatgcta
//
LOCUS       DAEPOG010000231         4575 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEPOG010000231 DAEPOG010000000
VERSION     DAEPOG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.233"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            79..1086
                     /gene="asrA"
                     /locus_tag="KR432_003426"
     CDS             79..1086
                     /gene="asrA"
                     /locus_tag="KR432_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBH0641932.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1087..1878
                     /gene="asrB"
                     /locus_tag="KR432_003427"
     CDS             1087..1878
                     /gene="asrB"
                     /locus_tag="KR432_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBH0641933.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            1891..2862
                     /gene="asrC"
                     /locus_tag="KR432_003428"
     CDS             1891..2862
                     /gene="asrC"
                     /locus_tag="KR432_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBH0641934.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            2919..3695
                     /locus_tag="KR432_003429"
     CDS             2919..3695
                     /locus_tag="KR432_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0641935.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            3966..>4575
                     /locus_tag="KR432_003430"
     CDS             3966..>4575
                     /locus_tag="KR432_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0641936.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSIS"
BASE COUNT         1805 a          415 c          879 g         1476 t
ORIGIN      
        1 gaagcataga aaataaagtg ttaagattta aacaagatag caagtaagta aaactaaaat
       61 ataagaaagg tgataaaaat gaaactaaaa ttagattcat ctaagttcaa tgaaggtctg
      121 aattatctaa aaaaagacta caagatattt gcaccagttt taatcccatt taaaggtact
      181 ttttcagata ctgatgttat aagatacaag gaggtaaacg cttttgaaga aatggaattt
      241 gctaagaaag caaacttttc accaaaagaa gttgtacttc ccataaatca agtattgttt
      301 tattttactg aaaaagagtt taaggaaagc gatttagaca acaagaaaat tttaattttc
      361 ttaagagctt gtgacataaa tggtataaaa agacttgatg aaatatatct aaataatggt
      421 gaagaaaaag attattttta taaaaatatc agagataaag taaagtttgc acttgtaggg
      481 tgtaaggaaa gttttagaaa ctgtttttgt gtgagcatgg attctaacaa aactgataat
      541 tattcaattg gattaaatat agaaggagat acactgtact tagatataaa ggatagtgaa
      601 tttgaagtat ttaatggaga agcaactgaa tttaatgttg attatgtaac tgataattta
      661 atatctgtag atgtgccaga gaatattgat tcaaatgaaa ttataggaaa ttcaatatgg
      721 gatgaatatg atacaagatg tataggatgt ggtagatgta attttgtatg tccaacatgt
      781 tcatgtttta caatgcagga tattttctat aaggaaaacg aaaatgtagg tgagagaaga
      841 agggtttggg cttcttgtca agtagatgga tatacagata tagcaggtgg aaattctttt
      901 agaaaaaaac aaggtgaaag aatgagattt aaaactatgc ataaaataca tgattttaaa
      961 aaaagattcg gatatcatat gtgtgttggt tgtggaagat gtgatgatgc ctgtccgcaa
     1021 tacatatcat tttcaaagtg tatagaaaaa ataaatgatt tagtaattag taaggaggaa
     1081 gtataaatga acccatatat accagtaagt gcaaaaatac tagatatagt taaacatact
     1141 gataaagaat ggacttttag agtaaactgc aatacaaaag gtgtattgcc tggaaagttt
     1201 tatgagatat ctatacctaa atacggtgaa agtccaatat cagtatcagg atatggagag
     1261 gattatattg actttacaat aagaaatgta ggaaaagtaa ctagcgagtt gtttaattat
     1321 aaagaaggag attctttttt tataagaggt ccttatggaa atggatttga tgtatccctt
     1381 tatgaaggaa gagaaattgt agtagtggca ggaggaagtg cattagcccc tgtaagagga
     1441 atagttgaat atttttataa taataaagaa aaatgcgaaa agtttaaatt aatagtagga
     1501 tttaaatcac caaaagatat tttatttgca gatgatttaa agagatggag taaaaaactt
     1561 gatattttag ttactgtaga tggagcagag gaaggatata gtggtaatgt aggattagta
     1621 acaaaataca taccagaatt ggatatgaaa gatataaaca atacttctat agttgtagtt
     1681 ggacctccaa tgatgatgaa atttacagta gaagaatttt taaaaagaga tttagatgaa
     1741 aagaacattt gggtttctta tgaaagaaaa atgtgttgtg gaataggtaa atgtggacat
     1801 tgtaaaatgg atgatactta catatgttta gatggaccag tatttgatta ttcatatgct
     1861 aaaaatctta ttgattaggg ggcgaagttt gtgataagag atttaaatac caaaaaagta
     1921 atgaaaaatg cttatagaat tacaaaaaca aaatatgaga cttctttaag agttagagta
     1981 cctggtggtc ttattgaccc tgagagttta gctgtagttt ctaaaattgc aagtgagtac
     2041 ggaaatggac aggtgcacat aactacaaga caggggtttg aaatattagg aataaatatg
     2101 gaagatatgg aagaaataaa taaaattata caacctgtta ttgaaaaaat gaatataaat
     2161 caaagtgaga aaaacgcagg ttatccagca gcaggtacaa gaaatatagc agcttgtata
     2221 ggaaataaag tctgtccaaa ggctcaatat aatacaactg aatttgctaa aagaatagaa
     2281 aaagctatat ttccaaatga tttacatttt aaagtggctt taactggttg tccaaatgat
     2341 tgtataaaag ctagaatgaa tgactttgga attattggta tggctttacc aatatatgaa
     2401 aaagatagat gtgtaaattg tggagcatgt gtaaaaaaat gttctaaaat atctgttgga
     2461 gcattaaaaa cagaaaataa taaggtggta agagataaag ataagtgtat aggatgtgga
     2521 gagtgtgtac taaattgccc aacaaatgca tggactagag atgagaaaaa atactataga
     2581 ttagctataa tgggtagaac aggaaagaaa aaccctagac tagcagaaga ttttctttta
     2641 tgggtagatg aggacagcat aataaagata atacttaata cttatagata tgtagaaaag
     2701 tatatagata aagatgcacc aggaggaaaa gaacatatag gatatataat tgatagaaca
     2761 ggatttatgg aatttaaaaa atgggcactt gaaggtgtag agcttccaga aataactaaa
     2821 atgtacgaaa acatatactg gagtggaatt aaatatctat aaattgagta aaacatatat
     2881 ttgaataaat taaaatttaa tagaaggaag agggaattat gcataaagaa actttggata
     2941 aattaactaa tgcagctata aataagataa atttattaaa cacaagtaaa gtaaaatatt
     3001 tggtatcttc tgcgtttgca ggactttatg taggtatagg tatacttttg atatttacta
     3061 taggaggact tttgactgat gcaggttcac ctatgactaa gatagtaatg gggttatcat
     3121 ttgcaatagc actaagtttg gttataatga cgggaacaga gttatttact ggaaacaata
     3181 tggttatgtc tgctggtatg ttaaataaag gtgtaagtat aaaagatact tctaaaatat
     3241 gggcatacag ttgggtaggt aatttaatcg gagctttggt tttaggtctt atatttgtag
     3301 gaactggtct agtagacaaa ggacctgtag ctgaattttt tgcaaataca gcagctagta
     3361 aagcatccat gccatttaca gctcttttct ttagagggat tttatgtaat attctagtat
     3421 gtgtatcagt gttgtgttcg tttagaacta atagtgatac tgcaaaaata attatgatat
     3481 tcttatgttt atttgctttt ataacatcag gttttgagca tagtgtagca aatatgacaa
     3541 tttatagtgt ttcattattt tcaccaacaa taagtacagt tactattgga ggagctattt
     3601 ataatttagt agctgtaaca cttggaaata ttgttggagg agctttattt atgggtcttg
     3661 gaacttatat attaggtaaa gaaaagctta attaaattta ttgtataaat tataactctc
     3721 ttgataaata catttttagt attattgaga gagttatttt tatatttttg aaaaaatatt
     3781 tgaaacaaaa aaaatatggg agcatacgaa aatatatcgg ttatatataa aaaaaatacg
     3841 tttatatatg aattatatag ttctaaatat aaaaaaagtt aaagtcctac cgtaaaaaag
     3901 cgcattttga ataagataac aaatatgata aaattggtct tgttgaaatt tttagggggg
     3961 atatgatgac aaagaaacta gtaatatcaa aaacaaagtt gtttattttt ataatagcat
     4021 ttatttctat atttatatat ttatttggaa gtaaaaatac acttataggt gtaggtatag
     4081 taacagctat gctaacactc ttggaaagag atttaactat ttctccaatt aaaaatttac
     4141 tgaagtattt agcaataaat ataatcttag gaatactatc attttttgca gtacaaaata
     4201 tgtatttagg agttttatta aattttatag cattatttat aatagggtat gtatttagtt
     4261 atgacttaaa gagagcagta tatgtacctt ttggtctaat gtatatattt atggtaagta
     4321 tacctgtagg aaaaagtgaa tttcctatga gattgtcggc tttagctgtt ggagcagtta
     4381 taataatgat tgctcaattt gttatgaata gaaataggat gaaaaatgtc ggagataaag
     4441 aactgatatc tatatgtgat gaattattag aaaaaattag tcttttaaaa aatactatta
     4501 atgatgatag ttctataatt aaatctagta tgagaaaaat agatagctgt aattatagaa
     4561 taaattctat atcta
//
LOCUS       DAEPOG010000232         4575 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEPOG010000232 DAEPOG010000000
VERSION     DAEPOG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.234"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..719)
                     /locus_tag="KR432_003431"
     CDS             complement(<1..719)
                     /locus_tag="KR432_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641937.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEY"
     gene            926..1210
                     /locus_tag="KR432_003432"
     CDS             926..1210
                     /locus_tag="KR432_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641938.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   1378..1671
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1445..1473
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(2332..4425)
                     /locus_tag="KR432_003433"
     CDS             complement(2332..4425)
                     /locus_tag="KR432_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0641939.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1598 a          687 c          435 g         1855 t
ORIGIN      
        1 ttatattcat tttcatatat atattttttc aatttttcgt aagatttata tgaatcctca
       61 tattttccaa tatgttttat gcaagcatac attccttctg gctttacata tagcttttta
      121 tcctttattt ttttatctat cttcaaataa aaacttgata tacttaaata gtccccattt
      181 tttatatttt cattacttac tattactccc aactcatatc cttgatacaa atttttcgat
      241 ttacaataat tggtgaaatc tattaattcc atcataaaat cactattact catattactc
      301 aaatcgcttg atagaactaa atattcttca aactttttgg atatataaat ttcttcacta
      361 ttcattttag aaattccttc aactgttata tttattctat tttgcatctt agagcttatc
      421 tttcttagtt tttttatttt ttctcttatc tttttttctt cttctttaag cattttcaca
      481 caaagctggg gagttctatt ttgtatatac tcttttattt cctctaaact cattcctatt
      541 tctcttaatg attgtaatat atcaagtgta tcatattgat aaactgagta atatctataa
      601 ttatttttat ctttatattc aggagaaaaa atacctattt tgtcatagaa aattaatgtc
      661 tgcttagata taccacacaa tttagcaaat tcacctgttg taaaatactg tttttccaag
      721 ggttatacac tccttttctt tttgttgtct atatagttac tatattcttt attatttact
      781 aaatcaagta gttattattt tagaaaatca atatggtttt gattttaaag cttaaatatc
      841 agttagatgt cagttataaa tattttatcc attcctctca taaaaaagat atcccctctt
      901 aacttttgga tggaatatta tatttatgct tgttagagtg aaaattgtaa taggtgctac
      961 aataagatat ttaattaaac ataaagttaa ttattgtaat aaaaaatctt tagataaatt
     1021 agagttaaag tatggaaata tagatagaga aaaagctgtt aaactagaaa tattttatca
     1081 atatttaata gaacttgaat atatagaaat gagtttatta ataagaagat tagatactgc
     1141 aataacatca ttagcattag tatctattat aacaatagca tcatattatt taatcagaaa
     1201 aaatgtgtaa ttatgtaagc tatttataat aaaacactga taaaattaat aataaaaagg
     1261 taataggatt tctatactac cttttggttt ttataatata gtgtaatttt attatatttt
     1321 tgtatgctaa tataactaga taatcataag ataacacatt aaaagaaaat tataaaaatt
     1381 tatattccgt atagttagta taaaacgtaa gtaatataaa tttatgtaaa acaacaaaag
     1441 atggatttac attccatata gttaatataa aacgttttaa tgaattaaca gaagatgaaa
     1501 gttttatatg tatttacatt ccatatagtt aatataaaac cattgaaaat agtgactttt
     1561 acgaaaaatt tgctaaaatt tacattccat atagttaata taaaacactt cctattaaat
     1621 ccacattagg aaattctccg agatttacat tccatatagt taatataaaa ccccaaaata
     1681 aacttagtat tttcaacact tacacgtata caactctcct aaatttgcag tgaacgagca
     1741 atagtgcaat tgataacgct tatcatgcac tctcaacacc ttatatttca actgttacgc
     1801 caaattttat cacaaatata gctcactgca aaatttctat aattttatta tatcataaat
     1861 atattacttt tgaatattga taccaattta taccataata aaagcaagaa aaaacgtaat
     1921 ctatctacat tggatttaaa ttctataact taaagttttt tacataatat ttatgcttgt
     1981 ttaatcaatc taatatatca ttcttgtata ttgcttttaa tagtcttttt tcttaaataa
     2041 atatctatct gaacaagcat agtttaatta aaaacttttg tattccctta aaactgtatc
     2101 tacctcttat tattttattt atgatatatt atttttaatc taaaaataga tatctatttt
     2161 tattatagtt atcccttttt aaaatacttt aatcatatac tactcctaat ttaacatccc
     2221 ttgaaatgct attttcttat attgcaattt ataattgata tatcttcaat ataataattt
     2281 aatgtatttt tgattttaaa gcttttcagt gatattattg ataaattaga attaattatc
     2341 tagctttaaa aggtcaaaga tttctgttgc tgtctgcggt tttccaaata aatatccttg
     2401 tatgtaatct ggctttattt gtttaactaa atcatactct tcttgtgttt ctacaccttc
     2461 taaacatact tttattccta catcatgaca aattgcaaca ataaaatgta taaatgtagc
     2521 atcaaactta ctctttaata tatctttaac aaatactcta tcaattttca ctatatctat
     2581 tggagaaaat tttaaaactt ctagagacga gtatccagta ccaaaatcat ccatagcaat
     2641 ataaattcct aaatcttgta aagccttaaa tttaaactgt aaaatactca tattttgtac
     2701 tgtatgactt tctgtcaatt ctaaaacaag attttgataa ggaaaatttt catcatctat
     2761 aatttttact atatcttcaa taaatgtgtc ttctaatatt tgaacagctg aaacgtttac
     2821 actcacttta aaaaaaggat agtaatcaat ccatttccta aaagtacgta atgccattcg
     2881 aagcacccaa gcccctacaa tatttatcat atcattttct tctaatattg gaataaattc
     2941 caaaggagaa attgctttgc atttgtcatt agtccacctt aaaagaactt ctacacctat
     3001 tattttatga ctttgtgtat ctacttgtgg ctggaatcga agactaaatc ccctaaaatc
     3061 atcattaatt gatgccttta aatcccgcat catttctaat gaatacattt tattttttaa
     3121 tatttcttgg gagaaaaaaa caagcctgtt tttaccatgc tctttcgcat attgtaaaga
     3181 gtaactagcg catttataaa gttcttttac tgtatctcca tgttttggat atattacaca
     3241 accagcagaa attgttatat ttaatccata ttttctccat agttgaaggt gtataatcat
     3301 attctgtatt tgattatata gagttagaat ttcattttct tcaacattat caacaagaat
     3361 acccatttcg tccccatcaa gtttataaag ttcagcatta tctggtaaaa tagattgtat
     3421 agactgtgct agtgttttta ttatattatc tccaaaatcc ctatcataca tctcattaac
     3481 atttttaaaa tcgtcaatat ttaataatac tatgcataat ttttcaatca taggatttga
     3541 aattttatct tcaaatacac tcataaattc atgataattc agtaactgtg tcaatgaatc
     3601 aatcttattt tgtttaccta attgcgtcat tattcctgca aaaatacttg gttctccaaa
     3661 ttcatcacgc attagttgtc ctctacatct taaccatata tattctccac tacgattttt
     3721 agccctaaat tccactgtat gataatccat ctggttttta cctatttcag tgtttgactt
     3781 ataaaaacga ttccagtctt ctggatgtac aatttttttc cagtaaataa gcggattttc
     3841 aacaatttct cctggtaagt caaataattc aacttgtgat ggtgaatatc gaaatacgcc
     3901 tgttttcata ttacaaatgt atatatattc atctgtacct ctaatcaaag catcataaag
     3961 caatgtagaa ttataatcaa aattgtctct tgaaaaatta aaaactcctt ttccttcttt
     4021 agtatttgat atttcattaa attcattaat cttaattggt ttatttaaga ttgttgagtt
     4081 tgcaatatct attgattgat gcatatgaac aactgtgcca tcttgccatg taattaaact
     4141 atcataattt tcaaaaacac gatttaactt attacatttt tcataccact ttattaatac
     4201 tcctttttta tcattcttta atagttctaa aactttacaa aaactacaag tagaattttg
     4261 ctcaggatat aatacttgcc agcaaacttt tccttctgga tctaaaatat tatattcttc
     4321 tttcattttc ttgttcataa aaataatttc attagtatga atatttgtaa catatatatt
     4381 aatctgtagt tgatttaata taacttcaaa attacgtttg ttcatatact tatctcctcc
     4441 atagttactg ctctttataa caacactgtt aaaattattc tttttagaag atttacttat
     4501 aacttataca tacacactaa ataccttaat ttaattagaa agtttattat ttcatatttt
     4561 aaattataat tgata
//
LOCUS       DAEPOG010000233         4520 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEPOG010000233 DAEPOG010000000
VERSION     DAEPOG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.235"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(21..689)
                     /locus_tag="KR432_003434"
     CDS             complement(21..689)
                     /locus_tag="KR432_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0641940.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            complement(682..1485)
                     /locus_tag="KR432_003435"
     CDS             complement(682..1485)
                     /locus_tag="KR432_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0641941.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            complement(1892..2314)
                     /locus_tag="KR432_003436"
     CDS             complement(1892..2314)
                     /locus_tag="KR432_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0641942.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            complement(2328..4505)
                     /locus_tag="KR432_003437"
     CDS             complement(2328..4505)
                     /locus_tag="KR432_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0641943.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
BASE COUNT         1438 a          834 c          469 g         1779 t
ORIGIN      
        1 ccatttttaa atattgaaat ctaatatgcc tttcctattc ttttttcaac tttgctttgt
       61 atattactca atattataga tactgcccag taaattaata tcgttatcat atatatctca
      121 aaatatttaa aacttgaagc agcctctact tgagctactc ccattaaatc ttgtattcct
      181 atagttagac ctaaagaaga acctttaatt atgtccagtg tacagcttac aataggaggt
      241 actgcagatg tagctgcttg aggtagaatt attttaacca ttgactgata tttacttaac
      301 ccaaaagcat aacatgcttc ctgttgtcct ttatcaacac tctcaataga tgctcgtata
      361 atttcagtca tatatgcaga tgaatttaca cctaaacaaa ttactattgc taaataaggt
      421 gaacaatctt tcataaaaga aaatatcatt ggaaatccaa agtaaaagaa aaatatttga
      481 attaaaagtg gtgttcctct aaagaatgaa acatacactc ttgttatgta ttgcaaaact
      541 tttgattttg tctgtgtgaa aaaggcaagt attacaccta ataatattga taaaaaaaat
      601 gcaacaatag atattagtat tgttattccc aatgctttaa tcaaaaatgg aaatatactt
      661 agacaaaatt caaaattaaa attactcatt aataggctcc ttagtaatgt cttttcctag
      721 ccaggcttca gatattttag tcaaagtccc atctttatgc atctcttcaa ttacttcgtt
      781 aatttctttt gtccattttt cacctttttc atttttttga atcgcaaaag aacaggctcc
      841 gaattgaatt ggaggttcta ccattttaag atttaatttt tgttctttta tagctattaa
      901 agcctcaaca tcctgcgcaa ctactgcatc taatcttccc atttctagat cttgataatt
      961 ccctttaaga gttgcacttg aataatttac tatttttatt tcatcatttg ggtacttttc
     1021 ttttagtatt tcatttgcta ctgctccagt acccacacct actttcttgc caactaaatc
     1081 atctattgat tttatactag tatcatcttc tctaacaata atgtttgttc cactataatt
     1141 ataagcatct gaaaaagcat attttttctc tctttcaggt gttgcagtaa gttgagcaga
     1201 tacaacatct gctttatcag cttctaataa tccaaataat ccactaaaat cagttaatac
     1261 ccattcaact ttacaattag ttcgtttagc aaattcatcc caaaaatcta cttcaaatcc
     1321 tttaagttta tctttatctt taaaagcaaa agggtaatat gttcctgatg ttgcaactct
     1381 tatagttttt tgttcttctt tatttttatt atttcctgat gaacatcctg taattaaaag
     1441 tgctaatacc aaggcaagca taataattaa tttaagtttt ttcataattt attctctcct
     1501 ttttaatctt attttaattt tttatataat acattttcaa aggttgaatc acttgtacca
     1561 ttataattgt ttaatggctc tgccataaca taactcccaa atattgatac tgtagtaaaa
     1621 gattgcctct tattttatac cttactaaat ttgcttttat aacactataa atcaattttc
     1681 tagttattat attatacttc gactttgaac aacataagtt taatatgttt tacagtcttt
     1741 gtccaatagc aaaaaactat tactaaaaat cacattatag tatccaacta tatccataaa
     1801 aaagctatat tgagttttta ttataacaaa ctcaatatag ctttttgtaa aaatatctta
     1861 taccaagtat aagtcactct ctatatttaa tttatttatt aaattttaat gctcctgtag
     1921 gacataccat tacacaaggt gaatccatac cacacttata tctctcaata caaccattac
     1981 acttatttgc cttaccatca tttccataaa ttaaggctcc atattcacaa gctttttcac
     2041 aaagtttaca ccctatacag ttttgattgt ctaatacaac gaatccttct tcttttttaa
     2101 aacaattttt aggacatgca cctaaacatt ttggctcatt acaatggata catgctaatg
     2161 aaaaatattc tatattttca ccttcctcta ttgtaaatac atgtctaaaa agttcaccat
     2221 tgtctatatt attatcattt tggtctatac atgcaagcaa acatgctcca catgctgtac
     2281 ataattcttt attaaattta aataaacttt tcattatatc tctccattta tctattcatt
     2341 ttttcaatct tacacttgca acttctatac gcagggaatc cactataagg gtcaaaatta
     2401 tcatcaataa tactatttat atcagattct ctatatccat gatacatatg tatattctta
     2461 tataagactc tatcagtaac attgactttt acatccaaac ttcctatctt agtagttatt
     2521 tttatataat ctccactgat aacacccaat tcttttgcat ccaagctgga aatatcacaa
     2581 ctagcttcat tttttaatat tcttgaaagt ttgatgtcat gaagtcttga atgtagtgca
     2641 tttgaaattc ttgctcctga tgtcaataaa tgagtatatt cattatctat attatcatta
     2701 aatcctctat atgttggtag tgcatccagt ccaaaatctt tatatttttc aataattgta
     2761 gattttaatt caaacctaga agtgctagta tcatacccct ctcttgtata tgagcctact
     2821 atatatggct taaaatcttt acattttaat ggtaaatcac tttctttaag tttatctaca
     2881 tctacacacg tctttttaaa catatgtctt atacttgctt cataacctgc ttttaataaa
     2941 tcatcatcta tattcataac atttgctaaa tctgaaagta tttctacatc tgattttgaa
     3001 tcgtataatt tatcaataac ttttttggta tatgtaatat accctcctgg gtaactctta
     3061 cattcttctc tctcaaatga tgtacacgca ggtaaaagta tatctgcata ttttgatgta
     3121 tctgtcataa ataaatctat attcacaaaa aaatctacac tatgcaacgc tttaaaaagt
     3181 ctccctgaat taggaaacat tctagcattt atcccatgac aaaacaaagc tcttatttca
     3241 tatggtgtct tttcttctat ttgcctaggt aaatccatag cttgaaattg agttaccatt
     3301 tcattccata gaggaaatct ttctccacca attctttcat ttatattttt aggtagaact
     3361 tcttttacaa actctttttc tagtgtttgg aatccaccta tcttaccagc aaagtaatct
     3421 tgttttggta tgtttccacc tattctatca taattacctg ttatagcaga taaagacatc
     3481 attgctctat agttttgaaa tccattttga tgatgaacta ttggagctgc actttcattt
     3541 atacaaaatg gaccttgtga caacatctca acagctttta ctatattatc tttagaaact
     3601 cctgtaattc tacttacagt atctaaatca aataatttta catattcttt atattcatta
     3661 aatccatgta catgcttttc tatgtattct ctatctatta aatccttttg tataaataga
     3721 ttcgccattc caagagctaa agctccatca gttccaggtt taattcttaa atgtaaatct
     3781 gctagttttt gagttgtcgg tgttattcta gggtctacaa ttataaattt tagacctttt
     3841 tcttttaatt ttattagatt cacactattt aaaaatttag agtagtatgg attaaatccc
     3901 caccctaaat aagtttttgc attctccata tcttgcattg taaagtttct tccagtagca
     3961 acatttccag ccatctgtgc tgatgttgaa catgaactag attctgtacc ataattaggt
     4021 gaaccaaatg aagatgcaaa cctttgcaaa aaaggtctat accactttcc ataacccgta
     4081 aaaaaagcta catgctttgc tccatactca gattttactc tatttaattt ttctgctatt
     4141 tctctataag cttcatccca tgatattggt ttaaattttc cttcacctct ttttccaatt
     4201 ctttttagag gtgtttttat tctatcttct ctatatatat attctctatt gcaaagacct
     4261 ttggtacata acaacccttt attcattgga tgttcttttg taccttctat ttttattact
     4321 ttaccatctt taacatatgc atcaattcca caatgtactc ctggagaaca aatatcacat
     4381 aaagtcctct ttatttctat acctgtttcc ttacagggta ttttagcttt aagttgtcta
     4441 taactatcca tattaacatt ttcctttccc aataataaat acaaattatt atacttatta
     4501 tacattcttt ttattcaaat
//
LOCUS       DAEPOG010000234         4505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEPOG010000234 DAEPOG010000000
VERSION     DAEPOG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.236"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            170..313
                     /locus_tag="KR432_003438"
     CDS             170..313
                     /locus_tag="KR432_003438"
                     /inference="COORDINATES: protein motif:HMM:NF014609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0641944.1"
                     /translation="MNKKFRLYQVDSFTKERFTGNPAGVITNADGLSESQMQKITREL
                     NNS"
     gene            335..1090
                     /locus_tag="KR432_003439"
     CDS             335..1090
                     /locus_tag="KR432_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HBH0641945.1"
                     /translation="MAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILNEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGK
                     MLVKVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
     gene            1315..1926
                     /locus_tag="KR432_003440"
     CDS             1315..1926
                     /locus_tag="KR432_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBH0641946.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            2224..2562
                     /locus_tag="KR432_003441"
     CDS             2224..2562
                     /locus_tag="KR432_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBH0641947.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(2969..4405)
                     /gene="miaB"
                     /locus_tag="KR432_003442"
     CDS             complement(2969..4405)
                     /gene="miaB"
                     /locus_tag="KR432_003442"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH0641948.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1633 a          631 c          524 g         1717 t
ORIGIN      
        1 tagcatagag ccttacataa tactactttt ttagatgtat atttttaaat tttaatatag
       61 acatatgtta ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa
      121 gttttctatg tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa
      181 atttcgtctt tatcaagttg actcatttac aaaagaaaga ttcacaggta atccagctgg
      241 tgtaattaca aatgctgatg gcttatctga atcacaaatg caaaaaataa caagagaatt
      301 aaataattca taaactgctt ttattttcaa taaattggct aaaaatcatc aagaaaatta
      361 tgatgtacat atcagatttt ttacaccaac taatgaagtt cctatatgtg gtcatgctac
      421 tattgctgcc cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca
      481 aaaaactggt gctggaatat taccagttga tattataaaa aaagataatg attatgaaat
      541 aattatgaca caaggaaaaa ttgaagtatc tccaccatta caacaaacct atcaaaaaag
      601 attacttaat gcatttggta ttttaaatga agatattcgt acagattgtc ctattgccat
      661 atcatctaca ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag
      721 tttatctcca aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggata
      781 ttatattttt acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc
      841 tgcaattggt atttcagaag accctgttac aggaaatgct aatggacctt taggtgcgta
      901 ccttgtacat tataaagtaa tacctatacc caacacagag tttatatttg aagcagttca
      961 aggagaagca ataaaacgca aaggcaagat gttagtaaaa gtaattattt cagataataa
     1021 gccagttgaa gtaaaaataa ttggaaaagc tgtaattgca ttctcaacaa acatagaaat
     1081 cgaattatag attctttcta agtaaatttt tgaacttttt aatactaatt taataacaaa
     1141 ctaatttata tacaatcaaa ttaatttttt tattatttgt taatatatat ttatcattat
     1201 attatataat aatatagata gttactatat cttataatta gataaatcat aattatatat
     1261 tattgttaaa actcaattat ataattttta ataatattat tttaggaggt aaaaatgata
     1321 aacgatatta aaaataaatt caaaaattac aaaccatata taaatggata caagaatatg
     1381 aagagagcat ctgttttaat tccaataata gaaataaata acacacacta tatattattt
     1441 gaagttcgct caaaaaattt gaagcatcaa cctagcgaaa tttcttttcc aggtggtaaa
     1501 atagaatcag gtgagactcc atatgaagct gttattagag aaacttgtga ggaacttgga
     1561 acttttagtg acaatataga agtaatatca agtcttgacc tccttataac accagtaaag
     1621 tttatcatac atccatactt aggatatata aaaaatatta ataatttaaa tataaacaag
     1681 gatgaagttg accatacttt tttggttcct ataaaatatc ttttagagaa tcctccaaat
     1741 acttatagta atactgtaaa agtttttcca gatgaaaatc taccatatgg ttcaattcca
     1801 aaccaaaaag attataaatt tgaaagtgga agttatccta ttctgtttta taaatataaa
     1861 gattatgtaa tttggggaat gacagcaaaa atactagaaa actttttaaa ttttttatca
     1921 gaataacact cataataaat catcaaaact tttacataag aaatttataa aagtttctaa
     1981 atttaacaat tctaaaatat caataactat attttgtagc acttttttat tttattattg
     2041 tttgaatatt tgatggtttt caatgcaatt tcaacataaa tactatgtat tagtgtatac
     2101 tgttgatgaa ttaaagtatg ataatctatt taataaagta tgaagtaaat gattttaatg
     2161 tgtctttatg gtaaaattac taataagtaa taaaattaat taaaatatat ggaggtgctt
     2221 ataatgtttg ctgtaggatt tgttgttata attgcacttg cctttttcat tttaagtgta
     2281 ctattattta atggaaaagc cagtatgcta ctagcaggtt ataactctat gagtaagaaa
     2341 gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt ctagcattgt tacttttatt
     2401 gtctctataa tgctatttat tatggcattt ttgggatata gaatagagag tggtcaaatg
     2461 gacgaaaaaa aaatggttcc cttttctata atatttataa tagtcatact ttccagtgta
     2521 ttttttaata tcctctactc taacaaaaaa tgtaaaaaat aaatagttgt aagttgagca
     2581 gttaattata aaagattgac tgctttttca tgtatattga ctactcttat taaatatttt
     2641 aacatttaaa aatctatgta aaaataatga atatagtaca aacttctaat aatgatttta
     2701 cctttgtggt tacagtccac tatatataat ttaatatgat aacatggaat tactaatgac
     2761 aactaagtaa gcttttataa aatataataa aacaaaaata gatgtacaat gaaattatat
     2821 cttatatcaa atttattttt attaatagca aaaacttaca ttagcataac atatgacaaa
     2881 ttcgcttaaa ataaaaattt ttaatataat ttgttctata gtacatctat ttcgattagt
     2941 ttacataatt ttattatttt gtacaatatt aattatttac taatatccca tttaatgaaa
     3001 aagttctagg ttcagtaatt ttgaccataa ctaatttacc aattaaatca tcatttccac
     3061 cttcgaaatt tacgagttta ttttgtctag ttctaccagc aaacttattt tcattatttt
     3121 tacttcttcc ttcaactaga acttcaacta ttctatcttt atatccatca tttatttctg
     3181 cagaaatcct atttacagct tctaacaccc tattaaatct atcatgcttt atatcttctg
     3241 gtatttgatt ttccatttta gcagcaggag ttccctgtct tttagaataa ataaatgtaa
     3301 aagctgaatc atatctaact ttttcaacca catctaaagt gtccaataaa tcttcttctg
     3361 tttctcctgg gaaaccaatc attaagtctg tagaaaaagc aatacctgga acttcctttt
     3421 tagctttttc tattatttct aaataatatt cctttgtata atgtctattc atttttttca
     3481 aaaggctact gcttccacat tgtattggta gatgtaaaaa ctcacacacc ttatcacaat
     3541 ctctaattgc gtatattact tcatcagata tatcttttgg atgagatgtc ataaatcgaa
     3601 ccctttcaat tccttctata tcattcactt ttcttaaaag ttctgagaat gaaactggat
     3661 tttcaagtgt cttaccataa gaatctacat tttgtccaag taaagtgatt tctttcgttc
     3721 catttgcaac aagctctttt atctcgttaa ttatatcttc aggagttcta cttctttctc
     3781 ttcctcttgt gtatgggact atacaatatg tgcagaaatt attgcatcca tacattatat
     3841 ttacaaatgc ttttaattca aattttctat tacttctaag accttctatt acttctccat
     3901 ccacatccca tacatccact aacattttgt cagaatttat tgattctgta agtaattgcg
     3961 gaaatttata tagattatgt gttccaaata taaggtctac atgtttatat ttttttctta
     4021 attcctctac aacatgtggt tgttgcatca tacatccaca tacagctatt ttcatatctg
     4081 gattttttcc ttttagatgt tttaattgtc ctaagttacc atacactttt agctctgcat
     4141 tttctctaac tgcacaggtg ttatatatta ttaaatcact ttcttcaacc atcattgaca
     4201 tttggtatcc catctcttct aacatcgaac ataatttttc tgaatcatgt tcattcattt
     4261 gacacccaaa ggtttgagta aacacgagtt ttctctttcc agttctgttg aagtattctt
     4321 cattttgtct gtgtatttgt tctatatatt tatcttgttc ttgtattttc tctataggta
     4381 ttgttacttg ttttcttttg ctcacgtcta ttcctcctag ttaatttctt atattacaca
     4441 taaccttaat tatatcaaat aagaaaaatc ttttaaagat attattattt ttctagttgt
     4501 gtttt
//
LOCUS       DAEPOG010000235         4452 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEPOG010000235 DAEPOG010000000
VERSION     DAEPOG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.237"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            101..466
                     /locus_tag="KR432_003443"
     CDS             101..466
                     /locus_tag="KR432_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641949.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            544..1098
                     /locus_tag="KR432_003444"
     CDS             544..1098
                     /locus_tag="KR432_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0641950.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            1318..1992
                     /locus_tag="KR432_003445"
     CDS             1318..1992
                     /locus_tag="KR432_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0641951.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2347..2443
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2497..2673
                     /locus_tag="KR432_003446"
     CDS             2497..2673
                     /locus_tag="KR432_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641952.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2861..3100)
                     /locus_tag="KR432_003447"
     CDS             complement(2861..3100)
                     /locus_tag="KR432_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641953.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(3245..4054)
                     /locus_tag="KR432_003448"
     CDS             complement(3245..4054)
                     /locus_tag="KR432_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641954.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1646 a          600 c          521 g         1685 t
ORIGIN      
        1 aacttaataa aacataaaat ttatatacag ttcatatata tttgtcatat cacaagacaa
       61 gtgatataat ttattatcat tttactgggg gtgatatgct atgaaaataa gagctgaata
      121 tacatgtcct cttgaaattg tacatgacat aatcaaagga aaatggaaaa ctatccttat
      181 atttgaatta agacaatcta ataaaacatt ttctgaattg caacataata tttatggaat
      241 aaatcaaaaa atgttgattc aacaattaaa agagttacgt gattttggga ttattgataa
      301 aataagttct gctggatatc cattacatgt tgaatatttt cttactaatc gtggtaaaaa
      361 aatgttaaaa gcagtggaaa taatgcaaga aataggaatc gaatatatgc ttgaacatgg
      421 tcaacaagaa attctagata ataaaggaat ttgctacaat aagtaatcct atacatacca
      481 aaaagtaagt aatttacatt ttttacaata gtattataat aaaagtaaat agaaggtata
      541 atcatgtcta tagctttaat attgattgat attcaaaatg attattttaa aaatggaaaa
      601 tgtgaattat ttcaatcaga agaaactgct gaaaatgcta aaaaaatact actgtttttt
      661 agaaaaaaca atttaccagt tattcatata cagcatatat caacagataa aacagcttct
      721 ttttttcttc ctaatactca tggttcagaa ataaataaaa ttgtttttcc actagataat
      781 gaagaaatta taattaaaca cacaccagat agtttctttg aaacagattt gcaaagtatc
      841 cttgagaaaa aaaatattac taatctagta atctgtggaa tgatgagcca tatgtgtatt
      901 gatacaagtg taagaacagc aaagaaactt cgatacgaca taactctaat aagcgatgca
      961 tgtacaacga agaatttatc ttggaataat atagaaatta atgcaaatac tgtacatcaa
     1021 gtattcatgg catcactaca gaacagtttt gcagatgtaa aaaacacaga taaattttta
     1081 tctaactatc acaaataaaa acatacacta tgttaaaaga gcaagtttga tttatttaag
     1141 cttgctcttt aatcatttaa aattagatgc tttctatcat ttttatatac ataatatacg
     1201 tttatacatt ttatattcat atataactat tatataaatt ttaaacttta ctatattcac
     1261 ataaaaaatg tagtattata gtatatataa tattatatag aagggggtgc tagataaatg
     1321 aaccatcata tattgattca atttgaatat atagcaagaa tcattactgc tggtttctgt
     1381 ggggctttaa ttggatatga gagaaagaat agactaaaag aagctggaat acgcactcat
     1441 tttatagtgg cattagcttc agcacttata atcattgttt ccaagtatgg atttaatgat
     1501 atagtgggta ctccaggtat tggtctagac ccagcccgcg ttgcagcaca agttgtcagt
     1561 ggtataggtt ttttaggtgc tggattaata tttattagaa atcaatcagt aagtggtctt
     1621 actacagctg ctggtatatg ggcaacagcc ggtacaggtc ttgccattgg ctctggatta
     1681 tacttagttg gtattgtttc aagtatcatt attgttattg tacagataac acttcataga
     1741 gataggaagt ggatgaaact ccctacagca gagcaagttt ctcttaaaat ttctaacagc
     1801 actgattcca tagaatatat tgaaggtaaa tttaaaaaat ctaatattga aattataaac
     1861 atgaatatta aacgccttga tggggattgg ttagaagtag ttatatatac taagttacct
     1921 aaaggatata aaaaaacaaa tttattaaaa ttatttagcg ataatcctct tatagaaact
     1981 ttagaaatat aatataaaaa ttgaagctta aaaataataa tatatttaat caaggtcaat
     2041 atataaataa ttacgggatg agtctatata aaattcatcc cttgtttttt gccattcata
     2101 tatatttttt caagatattt ttgctcatct attatctagc caagtaactc ttgtgaacta
     2161 aactttttaa tacaaaaact tcaatacaaa actaaatata atttttatta aatattctac
     2221 atgtatagtt tgtttcttga gtgaaaaata gatttcatat tgttaagata ctgttaaata
     2281 tttcaatttt ctgacaagta tgttgaacaa taacttatcc tagtgtatta ttttagttgt
     2341 agaataaaac taaatcggca aaactagaga aatttagtga cgcaaagcta tagggactaa
     2401 cgcttataaa ataaactatg agttatgtca gccagttgcc aaaaagatat tgttcttttt
     2461 gttttatgaa agttttttag ggggatagta atatatatgt ttagagatga aatggataaa
     2521 tgtacacaca tgttaactgc ttatattagt agtttatatg attattgtga ttttatagat
     2581 acacagctag atgattttat actagagtac ggagaaaatg tagtagaatc ttgtttacat
     2641 caagtgatgg tattggtaag taagtataat taaatagaaa ttgttttcta gagaaccctt
     2701 tttagtttta tctacattta aaaatgttat tatattaaaa aactaatctc ccctatatgc
     2761 aaagttttct tccatataac aatttcttcc catttatact ttttcacttg tatctttaat
     2821 aaaattcaac ttcaaatatt aatatacttc aaaaattatt ctatttctta tctttacacc
     2881 aattcctatc agtattatcc tttatatagg ttctaactat ttcgccacaa tctaaacata
     2941 aatctgaata tatagtagat tgtactggaa aagttttatt tataacttta tctaattttc
     3001 catctcctct atattttaat ccaatagttt cagcatatat gccaccagtt gttaaaatac
     3061 ccttttcaat atttacactt ccacaattag aacatttcac atttataacc ccctaaacaa
     3121 ttattttttc atgctatctt ataatttaaa atataatttg ctaccgattt tataacacag
     3181 tagcaaatcg gtggcaaatc aacatttttt cattctattt accatctaat ccattgaaat
     3241 atggctattt agttatagga acttgaattt ccgttacaaa ctcttctaca attccagtct
     3301 catgtatatc tatcttatag atttctatag ggtcagcaat tatcttataa ttattttctt
     3361 ctataaattt aaacaccttc tccatataca tcttattatt agaattactc ccagtataat
     3421 ttaaagttac ataatatcct tcatcaaaaa ctatattata tactttctca ttactttcca
     3481 gaaaacaaaa tacagattta aactcattaa atataccttt attaattgct tcagtagaaa
     3541 aggcagaacc aatattgttg tttccaagta tattaaatct atcttcatat tctttctgaa
     3601 gtttctgaat taaaaaatcg aagtcttcat ccctctttat atcagcattt aattctaaag
     3661 cttttctttt ttctatatat acaacctcta tctcatctat ttttgtattt tgaattacac
     3721 tttcaatcga actcaatctt tttataatat tttccttatg gcttataaat tcagctattt
     3781 ttttatcaat taaatcgagt tcttcattta aaatattttt cgtagactct atacttctgt
     3841 catctaggta ttcttttatt ttcttcattg gaaagttcaa gcttctaaat tcttttatta
     3901 aatttaattt ccatatatct gatatattat acattctata tccatttata tctctaactg
     3961 gtcttagaat gcctatttct tcataataca ttaatgaatc tctacctatt ccatatattt
     4021 tagatatctc tcctatttta tagtaatctt tcatttatat aataaactcc tccgaaatcc
     4081 tatttttatt ttaaatcaac attattactc tataatacta taatattaga atatactaaa
     4141 aaaatcttta aaacaaggat aggaaaataa acatcctaat aattaattat taaaatattt
     4201 ttatattgta atgttaggta tagtatttat aacttcaact tttttataaa tatattttag
     4261 ttttatatag ttttatgttt acagctatta tatccatctc tatgtaatat ttttcatatt
     4321 aaaactgatt tctatagatt aatagtttat aaatttagta aatgaatatt tattatagtc
     4381 tttaacaaaa aatacaagaa taaacttaat tttaataact aaaattaatt atgtggtatt
     4441 aaattatgta ta
//
LOCUS       DAEPOG010000236         4431 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEPOG010000236 DAEPOG010000000
VERSION     DAEPOG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.238"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            279..995
                     /locus_tag="KR432_003449"
     CDS             279..995
                     /locus_tag="KR432_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0641955.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            964..2556
                     /locus_tag="KR432_003450"
     CDS             964..2556
                     /locus_tag="KR432_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641956.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(2868..4370)
                     /locus_tag="KR432_003451"
     CDS             complement(2868..4370)
                     /locus_tag="KR432_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBH0641957.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
BASE COUNT         1586 a          619 c          543 g         1683 t
ORIGIN      
        1 tcatttgtta tttatatttg ttaatatatg tttaaaccta aaaaattaat aaacccaaaa
       61 taacataagg agtgtgatag attaccctag atattttcat aaacttttac cttcagatta
      121 atatgggcgt ctatactttt aaatttacta tatgcattga caatatatta aaagtactag
      181 tttttatttc ataatgaacg ggcttactgt tttctaatac ctctttttgg tataaaataa
      241 aaattggtta aaaataaaat ttcaaggggg ttaataaatt gaatcaaaat atattggttg
      301 tagatgatga tagagagata gttgatgcta tagagttcta tttgagacct gataacttta
      361 atgttttaaa ggcatatgat ggacttgaag cattggacat actaatcgaa aatgatatta
      421 agttaataat aatggatgta atgatgccca atttagatgg gcttaaaact acacttaaaa
      481 taagagaaaa aaggaatata ccaataattc tcttatctgc aaaatcagaa gatatggaca
      541 aaattatagg tcttaatatg ggtgctgatg actatataac taaaccattt aatccactag
      601 aacttactgc tcgagtaaaa tctcaactta gaaggtatat aaatttaggt gattttagta
      661 atacaaataa ctcaaatgat actaattata ttctaaaaag tggtggccta gagctaaata
      721 cagatacaaa aatagtttct ttagatggtg aggaaattaa aatgacacca cttgaatata
      781 aaatacttac acttcttatg tcacgaaaag gtaaaatttt ctcttcccgt gaaatttatg
      841 aaaatgtctg gaatgaagat gcttatagtt gtgagagaac tattgctgtt catattagaa
      901 gaattagaga aaaaattgaa attaatccaa aagaaccaaa atatttaaag gtggtgtggg
      961 gcattggtta caaaattgaa aaacttaatt attaatgatt ctaaagcaaa atttatttta
     1021 ttattattac tgtttctatt agtttctata ttatttgaag cagtttatga tttgaataat
     1081 aacttttctt ataatattaa ttgttattat aaagagtttg ctctccctta tttttatttt
     1141 ttcggtactc ctgtacattt gtcaaaagta gaattagagt ttgtctgcat aggtattata
     1201 ttatcaagtt ttttattgac tatactatgt tttacattgt ataaaaaaaa taaacagact
     1261 aaagtcaaat tcttagagaa tgtcataagc cttttttcta aggtaaaaat agaacttaca
     1321 cttatattaa ctttgatact attaacctta aggtttcatg catttacaag taataaaatg
     1381 ttttacaaca ctaatttaat aatctacaat tttataatta tatctatagt ctatataata
     1441 tatgaaaata ttaaatataa taactataat ctaaaagata tgtctataac atatgtattc
     1501 ttttcaaact tacttagtat gtataataaa aaaactataa ataaaaaatt agctatagta
     1561 tttttaatat ctatagttgt acaagctatt atccaatatc ttgttttgat attctttatg
     1621 tatatatatt attctgaggc aatagctata gtaattttat ttacacttgt aaacacagtt
     1681 tttcattttt atatatacaa ctttttatct gttaagttta attacataaa taacatatca
     1741 aaaaatataa aaaatataga aaatggcaat ttaaagtata aattagaagt tataggagat
     1801 gatgaaatat ctactttagc caaaagtata aataatataa caggtggtct tgaaactgct
     1861 gtagatgcta atataaaaaa tgaaaggatg aaggcagagc ttatcacaaa cgtatctcat
     1921 gacttaaaaa ctccacttac atctatatta agctatgttg atatgttaaa aaataatgac
     1981 tttgacagag agactataaa tgactatata aatattttag ataaaaaatc acaaagatta
     2041 aaattattaa tagatgacat atttgaagca tctaagctaa gtagtggaga tgtagaattt
     2101 aatataacaa aaaccgatat aagagaactt ttaattcaaa gtattgtaga gtttgaagac
     2161 aaaattcaaa atagcagttt agattttata atagaaacac ctgaatatgc tgtttttacc
     2221 atgatagatg gtaaaaaaac ttggagagtt tttgagaatc taatttgcaa tatactaaaa
     2281 tactctatgc ctaacactag ggtctatata gatatgttta taaaggaaga aaatataata
     2341 ttaacgttta aaaatatttc aaatgataaa ctaaatctaa agccagaaga gttaatagaa
     2401 agatttagaa ggggtgatat atctagaaaa acagatggct ctggtcttgg tttatcaatt
     2461 gctcaaaata taattgaatt agaaaatgca cacatggaaa taattataga tgctgattta
     2521 tttaaggtaa tgcttacttt tagaagagtt aattgatata ttaatataaa aatatatttt
     2581 aagttattta aacaagaaaa aaacctcctt agaggtcttt ttctaaatgt tggggtttat
     2641 atttacatat tttttgatta tacctttttg gtgtgatatt aatacatttt aaattatatt
     2701 tctctataat tcatcacata tttcatctct ctgtatctat aatatcacat agtgtttcca
     2761 taatcagtta catctctgtt acctaagatt acaattttgt aatatttgta atcttaggta
     2821 caaatttaca tttgttcttt aatataaata tattctttct atttacccta aagacctttt
     2881 aaatcatatt tttctaagaa aagttttaac ttagaataaa tttgttctgc tccaccaatt
     2941 ttatttgatt caatatttaa agctagtaat ggttcatgta gtgatattct aagtaaaaac
     3001 catccatctc catttgattc atcacaatta actcttattc cttcaaaatt atttggtgct
     3061 acagaccatc catcaatact cttaacatga gaatctaaat cttttaatat atcctctgca
     3121 tatggtctaa aatcttcttt gttaatgcct attctaactt ctttctcttc ttgagcttct
     3181 tcaaggtttt ctatcaattc ttctatagtc tttccttcca aggatagctt tgctactttt
     3241 attaaaattt tagctattaa atatgcccca tcatctaaga aataattttc ttttaaagca
     3301 gcatgtccag acgtttctat agctaaatgg cactcttcac cttcactatt caatcttata
     3361 gcttcattta ttacattttt atatcctctt ttaaatctat gatgtcttcc ttgtaattca
     3421 tttataaatt tagcaagtcc ttcagaagta atcgaatctg ttactatagc tgtttttgga
     3481 tgttcttcta atactattga tgatataaca gctataagag catttttatt tattggtttg
     3541 ccatttttac ctacaatagc agctctgtct acatctgtat caaagattat tcctaggttg
     3601 ctattattat ccaacacagc tttacatatg ctttccattg cttctttgtt ttcgggatta
     3661 ggtatatgat ttgggaacat accatctggg ttcaaaaact gactgcctgt cgtatctgct
     3721 cccagtatgt gtaaaacttt ttcagcaaag aatccaccag ctccatttcc tgcatcaacc
     3781 aatatcttaa gcccagaaaa aggcttttca taatttttac ttgaatttac accttttcta
     3841 attttatcta tcaataaatt agaataatct tctattaaat tttttactat aacttcacct
     3901 tttttattta cttcctcata gtctacacta tcatttttaa cagctatatc caacatctct
     3961 tttatattag ttttttctaa acctccactt tctgtgaaaa actttaaacc attatagtaa
     4021 taaggtaagt gacttgctgt aatcataatc gccccatcag aattgtatcc atccataata
     4081 gttgtcataa acatagctgg agttgtagca atctcacaat caattacatt acaatcatca
     4141 ttagttaaag tttcaataac agtacttcta aattggcttc ctgttatcct agaatcagtt
     4201 cctatggcaa cagttacttt atctttctta gtcttttctt ttagccaaat atgaaacgct
     4261 ttagctattt tttttacttc ttctacagtt aggttaactt ctttagaatc attttcatac
     4321 gctattcctc taatatcagt accattttgt agtttatata ggtctcccat aaaaaattct
     4381 ccttactcaa ccaaaatttt tattaaatat tataaaattt cttgtttcca c
//
LOCUS       DAEPOG010000237         4413 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEPOG010000237 DAEPOG010000000
VERSION     DAEPOG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.239"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            230..1309
                     /locus_tag="KR432_003452"
     CDS             230..1309
                     /locus_tag="KR432_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0641958.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="KR432_003453"
     CDS             1633..2001
                     /locus_tag="KR432_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0641959.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="KR432_003454"
     CDS             2118..2921
                     /locus_tag="KR432_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0641960.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="KR432_003455"
     CDS             3306..4196
                     /locus_tag="KR432_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0641961.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT         1674 a          488 c          840 g         1411 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gct
//
LOCUS       DAEPOG010000238         4345 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEPOG010000238 DAEPOG010000000
VERSION     DAEPOG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.240"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            534..728
                     /locus_tag="KR432_003456"
     CDS             534..728
                     /locus_tag="KR432_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBH0641962.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(830..1339)
                     /gene="vanZ1"
                     /locus_tag="KR432_003457"
     CDS             complement(830..1339)
                     /gene="vanZ1"
                     /locus_tag="KR432_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBH0641963.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1659..2049)
                     /locus_tag="KR432_003458"
                     /pseudo
     CDS             complement(1659..2049)
                     /locus_tag="KR432_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2493..3062
                     /locus_tag="KR432_003459"
     CDS             2493..3062
                     /locus_tag="KR432_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641964.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            3354..3671
                     /locus_tag="KR432_003460"
     CDS             3354..3671
                     /locus_tag="KR432_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641965.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT         1679 a          539 c          487 g         1640 t
ORIGIN      
        1 tactactctt tatatgtaat tttctactat atatatatat ttattctata aaataatccc
       61 ctttcaaaac acttttctta aatcactcaa actaaaatat atataagatt atcattagtc
      121 agtaagtata tatttttcta atttctaaaa atacatccaa atcatcaaac ttttttagca
      181 attactttat tattatacag tgtcaaatca aaacttctaa ataaattgtt attcatatag
      241 caaacaaata cttcgcatca attttttaat ctctatttaa atagtatttt ttattttgtc
      301 attttataga tattaatgtg tatttaagac tgatgaaaaa taataaaatt ttataatcta
      361 ttcaataata tacttttttc tataaatatt ttacccataa cctttatttt tttattaaaa
      421 tgcattataa taaaaagtaa atctaaattt gatacagttt taacaatcat tattttcaga
      481 aaagtgatac tattgaatat attaagaata attagagatg gaggaactaa gatatgagcg
      541 taattgtaat gtgtagtcgt tgtggaaaag aaatagataa atatagaaat aaatatataa
      601 cttattatga gaaaaatacc aatgcagaaa ttaaagtttg tataaattgt gcattagatt
      661 tattagaaaa aacaagagaa aatcaagaat tgaacatacg agagtttcaa gatacaataa
      721 ttctttaata atttaggaaa gcacctatat aggtgctttt tatgtgtaaa taattttata
      781 tgtaactgta tcttacgttc tattttaagc taaacctata tacttatttc tataagtttg
      841 caaaaataac taatcctaaa aataatacaa caataattgt cacaatcaat gccaatacat
      901 tgataatttt tatggtatta ttcttaaata ttttcgataa tagcataaaa acagctattc
      961 ctatgattcc tcctagtgtg tttcctatca aatcggttat atcacttgca cctagtgcaa
     1021 atatatactg aagtgtttca aatgctaaac tagttataaa aataggtata acttttttta
     1081 taaagctcca ttcttctttt agcatacaca catatatccc aaatggtaca aatatagcca
     1141 catttaatat aatctctgat atatctactc tattattaat tataagtgac ccagcaaaag
     1201 gtactaagtt tatgcttcta agattcatgg tttcaagaga tgagatatca aattgcaact
     1261 taaatagtat tatccatgtt aaaataatta tgtaaactat aaataatcct tttgtaatat
     1321 tatgttttct agatttcatt tattacctcc ttactatatt attgtattat tttattaata
     1381 caataatata gtaaggtaat ttatctgtca actatttctt ctaaaattat attcctttta
     1441 taatttcaat atctttatca tatttacatg atttaagcta ttgtagtatt tcatatatct
     1501 ttaaatttta attggtatta ttctctatat tttttactat caaacactaa ggaaataatc
     1561 acttttcttt atataatgtt ttaccatcac ataaaactaa cattaaatcc aaacgctact
     1621 ctcaattgtc tcaaactaaa aagatactga tagctttatt agtcatcaat atcttcttct
     1681 ttcaaatttt taaagtattc ttctaatttg tccaagctct tactatctaa aacttcatca
     1741 tcatgtagtg ctgaaataaa actcttaaat gaattgttat gcataaaact aaaaaaactc
     1801 tttgtctcaa cttttaagta atctttctct tttatagcta tagtataatg cgtacatcta
     1861 tctaccttaa taagtagtta aaaatccctt atctactaaa gtttttaaaa atgtcaatgt
     1921 tgtgctcttt ttccaatcgt atttcaactt cattgcttct atgatatctc ttgaggctaa
     1981 tacatcatct gctccccaaa tatacttcat tagttttagt tctgcatgtg gtaatttatt
     2041 aatcttcatt agtttacacc tcttaccaat ttatgtataa ttaaatttta tactaataat
     2101 agtatgtaat caatcagatt ggaacaaata taatattcca atttattcca tataaaaata
     2161 attaataaaa aagaagtact ctactttaga gagtactttt ggattatata ttcaaaaatt
     2221 tatctaacat gattagagta tgttttatgc tttagtgtga taattttagt tcgttttatt
     2281 attataatat gtattatatt tcattataat ctataagctt aaatacagga tatttatttc
     2341 atatacttaa tttatattaa gttttattat atgtattctt aagttttact atatttttta
     2401 ttaatattta atgtagttaa ttcttaagaa ttatattcta caataataat atatatcttt
     2461 gatatataac taatttaaaa tggaggtatt ttatgaaact aagaaatcca aagattaaga
     2521 aattgttatc agtagcatta ttagcagctc ttataggtat acctattgga caaacagcca
     2581 atgcaatgga aatttcaaat aacgtcccaa gttatataga aaacaatcca acaacaatag
     2641 aagacttgga aatcaatgac tctcaattac ctaaaccagg tacagttaca tacatagata
     2701 ttaataagct aattaaaaac cttaaggaaa atggtatcga cactactctt attgaaaatg
     2761 aattatatta tagaaaagct ggaacaaata aaatagtttg gactaagaca ggctttgatt
     2821 tatatctttc taaaggagtt gcattaactt tagctggtgc tggtacaggg gttgcagggc
     2881 ttttggctgg tgccattcct ggtataggac agtatcttgc agctgctata gttggagcaa
     2941 ttacaatgca tcttggttca aatataacat ctggaaaaat ctttaaattt aaaaaaatgc
     3001 gttataataa aggtggggct tatgtaacca aatattactt acaatctgta agaaatcaat
     3061 aggtgataaa atgattataa aatatctact ttttgcaatt atctcagcac tagttttatt
     3121 cttttgtaat aaagtatttc aactaagtaa attacacaag ttcattatat tgtttgtttc
     3181 attttttata ctaacttttg tagcatcatt tattaataga taaaaaataa gatggtgggg
     3241 ttaacctacc atctttttct catttaatat ttgtaatatt ttagatactc tttatacttg
     3301 tgttactgat atactttgat taagaaaagt aaatttaaca ggaggtatct gctatggatt
     3361 ttggaaatat caatcttata cttattggaa taatagttat aataggaact acaataatat
     3421 atttaattaa acctaaaact gctttttgta gtaaaaaata ttttaataaa ttagaatcaa
     3481 tatatggaaa tatagataag aaaaaaactg ttaaactaga agtactttat cgctatgtaa
     3541 caggtcttga gtatatatca ataggcttat ttacaagaag attagatatt acaataatag
     3601 caataatatt agtagctact ataacagtaa tattatatta cttagttaga aagagatata
     3661 taactatata aaatctaata gaattaatat caaaagaaag tagcaactaa tcaccagtaa
     3721 tgtaaaataa tttgtttttc aaagttaata aaagtcttct ttatggcaat gatttaaata
     3781 taatcaaata tcgtaacgga gattttttta tgaataattt aagaaaacaa ttaatcaaag
     3841 aaattatata agatggagaa cttataaaat ctacagatat aagtagtttt aggagtaaat
     3901 acagatgact tatttcaaag agacaatagc tacaagttat cctcattctg taatacaaaa
     3961 atgtttaata cattagataa gaaatacttt aaatatgttt catacaagga ctcaaaagaa
     4021 ttaataagtg attttaaatc tgtatatagg tcagttaatg aataaaaagc cttatcgact
     4081 ttagataaaa tagattaaaa ataatgtaaa cagtatactt cagaaattaa aacgtttgga
     4141 aataactgga atgttgtagc atcagccttt agttttacta aaataagtaa tatctataat
     4201 atatattaca agtataatcg aaggatttaa taaatatttc aaaaatgtct caaaaacaaa
     4261 gtccagattt acatcaaacg cttcactctt aaagatatta ttcttgacaa gcgaaaatgt
     4321 gatgaaaaaa tggactatga aatat
//
LOCUS       DAEPOG010000239         4271 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEPOG010000239 DAEPOG010000000
VERSION     DAEPOG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.241"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(473..1291)
                     /locus_tag="KR432_003461"
     CDS             complement(473..1291)
                     /locus_tag="KR432_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0641966.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1459..2040
                     /locus_tag="KR432_003462"
     CDS             1459..2040
                     /locus_tag="KR432_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0641967.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2037..2231
                     /locus_tag="KR432_003463"
     CDS             2037..2231
                     /locus_tag="KR432_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641968.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2395..4137)
                     /locus_tag="KR432_003464"
     CDS             complement(2395..4137)
                     /locus_tag="KR432_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH0641969.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         1536 a          553 c          551 g         1631 t
ORIGIN      
        1 gatataaaat attgattaat aacatatatg ttattggtga acatatttct atagaatgga
       61 atatataatt ttttatattt atcattgtat tattatacaa agaaagttaa aaagagtaga
      121 gtacaaagga attatttgta ctttactctt tgttttgtta aaaactaatt tatattagtg
      181 attcaaatat ttaactagag tataagggtt gtatataatc ttataagtaa tttatctgac
      241 aaattaagat aatatcctaa tcaaattcta gtcattcgaa cataacatca caagtgattt
      301 atctgacaaa ttaagataat atcctaatta aattgtagat atttatacat aacactaaaa
      361 gtaatttgtt taacaaatta agataatatc ctaattaaat tgtaggtatt tatacataat
      421 actaaaagta atttgtttaa caaattaaaa tattatccaa ttaatataaa aattacttct
      481 ttttaactgg gaagcatacc tctgttaccc aattatcagg attactatcg ttgccaggac
      541 ttacatgata aatattaaac ataggaccat ccacatcata gttattatct gatatccaat
      601 taccaatagc ctcacaagcg tctgcaactt gattgaaatt tccattaaca attgcagttg
      661 cagcagtcac acttggaaca gtcttaaatt taacatgttc agtatcttta tactttccag
      721 atacagcaac ttgtacttca acatctacag aatcttcttt atatccagta tcataaaaga
      781 ctgctttaga atactttggg ggttcaattt gaacattctg gtcttttgtc tctaaaaatg
      841 cttgatgcca taacattcct tcgtcttgat aagttggtat aacccttcta agtgtcatca
      901 tatattttgg tgcaaaattc tttatagtta catcataatt tttcataata tcatttcctc
      961 caattctttt aatagtggtt tctattttta atagtttttt ctgtgtatct tctaattgtt
     1021 cttttacttg agaatggtgg atatttagat attttataag actttcttta tcgttatatt
     1081 ctgtcaaaat ttcttttata gaatacaagc caaagcccat atcttttagt gcatggattc
     1141 tatttgtagt agaaagttgg tctgcactat agtatctata tccattagtg ttattcgtat
     1201 gagaaggagt taaaagtcct atttcatcat aatgacgtaa catacgaata ctaattttac
     1261 ttagctttga aaaatcgcct atcttaaaca tgtttttgtc tcctttataa aggtactttt
     1321 agcttaatat taatataaac catatcataa tgttagagtc aactaaaatt ataattaaat
     1381 tatattgaca tatgatgaaa aaaatattat actgaacatg ttcagaacct aaaatgttaa
     1441 ttattttgga ggtctattat gaaaagtaat gataaagatg ctagagaacg tataattgaa
     1501 gttacattga atcttttaaa tgaagtagat gatattgagg agattacagt tagaaaaatt
     1561 gctgagcgtg caaatgttgg agttggttta ataaattatc attttaaaac aaaggataat
     1621 ttgttgagta ctgccatagg agatgtgatg tcaaatataa tagcagagct atatgacgat
     1681 agtgtatata ctctaagacc tattgaagat ttgaaaaatt tattaaaaaa attgtgtgat
     1741 actggattac attatgaaaa agtgcttcct tttgtgttga atcaatgtat aaccaatgga
     1801 gatatgcaag cagaacttga tattgtccct atgcttcgta aaatttttgg aaataaaaaa
     1861 gatgaaatga gtctaagaat cattgccctg caaattattt taccaataca gatttctgct
     1921 ttatctacag aatcatttca gttatacagt ggtataaata taaagaataa atatgagcga
     1981 gataaattca ttgatatttt aatagaaaat attattggag aggatgttga tgtaagatga
     2041 aaattataac tattggttca agtcttatca ctgttttact ttttctttca actatggttt
     2101 gtgggttctg gattaagaat aataaggtaa cagatgcaag ttctattaag ttccatatga
     2161 atagtgctat ttttacaggg atatttttat tgatttccac aatcctttta attatatata
     2221 taaaaaaata ataatattat taataaataa agtatcagaa attataaaga caagagtctt
     2281 aaaaatttct gatactttac cacataattt tattaaacat taattgtgtg tgaaaaaata
     2341 tcacttattt caatctaata ctaaatctta agagtgagta taaagtatta gaaattaaga
     2401 agcatttttt agttcaagtg gattatctat accaaaataa tcatataaag tagagcttat
     2461 ttcctctaaa tttccatctt ggaaaggtaa ttgtaaattt accttttcta ccacatcttt
     2521 aaatccagca tcagtatttt gatttaaaaa atccatatat acatcaacag cttttctgta
     2581 atcttcttgt gataattgcc atatctgaag tgatacatca gatgcaagac cataactcaa
     2641 gtaataaaaa ggaacttgga agttatgaga tacaagtatc caaaaaggag catttttttc
     2701 tctaagtata ttattggaaa ctccatattc ttcctctaaa tcaaaaaaca atttattgat
     2761 atcatttaga ctcatatatg gattcttgta tataatttct tgaaattcat cataaagaca
     2821 agcatcaata atagtattta aaacaatcga taagtgttct ttttttatag cttcagattg
     2881 tttaccaaaa aaatcatcaa aatatttata aaatagaatt tctaaagatg ttgaatgaac
     2941 ttcacataca tctatacttg gttgcatctt attatttaag ttaatactgt tgtagtaatt
     3001 ataaaaatga ccaaactcat gagcaaaact agtaacacct aaaaaagaat tgtcccatgt
     3061 attaaacaag aaaggtgctt tatatttatt tatataagta gtatatcctc caggcgattt
     3121 gttttttcca gaagaataat cgtacaagtc atattttttc atatagtcga agctctcttt
     3181 tagttttgga gatatatctt caagtacttt atcaaacttc ctaaatgagc gatttttata
     3241 tactttaata taaatagaag aatcagatgg tttagatgaa atctctctat ataatggaac
     3301 tatgtaatct tttacatctt gtctaaattt tttagcttct tcttgagaat agtctttgtt
     3361 taggttcata taggcataat ctatataatt cttaaatcct tgcttacttg ctatttcagt
     3421 tcttgtctgt ataagttcta agaatatgtt accaaatata ggattgtatt tttttaagta
     3481 atctgaataa attttcacat attcttcagg gctaagattt ggtcttgaca aggcttcttc
     3541 aaagtctacc tctataccat ctattgatac tgtagattta gctaataagt cttcatattt
     3601 tacaactagg tcttgctctt ttttaagtaa atcttcaact tctttagaat taagttttcc
     3661 aatgttcttt agataattaa atgtatcctt tccaagttct ttttctaatt ctttagagaa
     3721 tttagaagtt acaaatctat cttgaaagtc tttgtatgac atgtcaacat ctactgattt
     3781 actcataaga tacttatatt ccttagagta aaattcgtct gatgaatcta tgttatttct
     3841 aatttttgct atagtaagtg tactgtaaaa atcctgataa attttatcaa cttcattaaa
     3901 taaatttaat tgttccttag ctgttttagc tgttaaaagt ttgtcgttac acttgttaat
     3961 gttttcgcaa attgactgta tattaggacg cttatattcc attttttcaa aaggaatagt
     4021 tcctctaaca gaatcttttt ttgtatcatt agtataattt cttatgtatg atgcaaatat
     4081 aataaaaact gcaattaaaa ttagaaaaac aaatattttc ttcttggatt taaccataaa
     4141 aatcctccaa tcatggatat agttattaat tattttacta tattaaatat aaaaaatgca
     4201 tgttaagaaa atttaacata aagatataga taagattata aataatataa aaaatttaac
     4261 ttcaaaaaag t
//
LOCUS       DAEPOG010000240         4246 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEPOG010000240 DAEPOG010000000
VERSION     DAEPOG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.242"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(189..494)
                     /locus_tag="KR432_003465"
     CDS             complement(189..494)
                     /locus_tag="KR432_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641970.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(1216..1434)
                     /locus_tag="KR432_003466"
     CDS             complement(1216..1434)
                     /locus_tag="KR432_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641971.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(1435..1842)
                     /locus_tag="KR432_003467"
     CDS             complement(1435..1842)
                     /locus_tag="KR432_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641972.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1928..2416)
                     /locus_tag="KR432_003468"
     CDS             complement(1928..2416)
                     /locus_tag="KR432_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBH0641973.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2596..3504)
                     /locus_tag="KR432_003469"
     CDS             complement(2596..3504)
                     /locus_tag="KR432_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0641974.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            complement(4055..4225)
                     /locus_tag="KR432_003470"
     CDS             complement(4055..4225)
                     /locus_tag="KR432_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641975.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
BASE COUNT         1443 a          668 c          375 g         1760 t
ORIGIN      
        1 ttaactattt tatattataa aacacctttt aacttttgct aaaaaatatt ttatacataa
       61 aaaagaaacc acattctagt tttatacaga atatagtttc tttaaaaata atcaaattgg
      121 gattttaaat aattaaaaac aaacttttat ttgtttttaa ttttccaatt ttatagtaga
      181 taatttagtt aattagttct taaatgttta tcttctttag taaatttttt tcttattaag
      241 aaatatgcaa ctattacaac aagttctatt gctaaaaata caccataagt agctatagaa
      301 tctatgctat ctatgccatt tctaaagaaa attaaagcaa ttcctgctgc tataatacct
      361 acagcatttt tccctataga attttgacca aaaccataaa aagaagggaa atttcctttt
      421 atttcaatga atgtcaagat taatccaata gctataaata gtactcctac aacttccata
      481 atcatgctag gcattttgac ccctccttat taataattaa ctactactat aaaaatattt
      541 attattttta taattatatt atatatgcga ttacatgttt ttggaagtat atttggaagt
      601 aatttcatat ttatttaact taattttttt gtgataaaat aattctaatt ttcatataga
      661 tattattata aattttctag tttatggagt tattcattct ataattcaca aatatgtatt
      721 ttttaatcta ttaaagtatt cattttctgg cttataagca tggacttctt atctataagc
      781 ttggttttta tttataatta attcattctc taatctataa gcttggtttt tatttataag
      841 tcatttattc tctaatctat aagcttgttt tttacctata attaattcat tctctaatct
      901 ataagctttc tttttattta taagtcattt attctctaat ctataagctt acttcttaat
      961 ctataaatca ttcatttttt aatctattag cttgtttttt acctataatt aattcattct
     1021 ctaatctata agctttcttt ttcatctata aatcattcat tctttaatct atgagcttgg
     1081 tttttcatct ataagtcatt tattctctaa tctataagct tggtttttta cctataatta
     1141 attcattctc taatacataa acttgaattt tttatctatt aattcattct ctaatgcata
     1201 agtaatacat gaaatttaaa tattattttc ttcaataaat tcaaacaact cttctacagt
     1261 tgtatcaaat accttagcaa tatccattgc cagttttaaa gaaggattgt agcgaccatt
     1321 ttctaaatgt acaatagttt cacgtcttac tcctactgac tttgcaagtt caccttgtgt
     1381 cataagattc ttttctctat aggatttaat ttttgtcttt agtaatactg acacctaatt
     1441 gtatatcccc tttctgtcaa aatatataaa tagaattaac ttcaacaagg attgaataaa
     1501 caaaatgact aataaagaca ttcctatatt taaatttgac acatttatgt ccaaagttac
     1561 aagaaatata gatactatag ctacagaaaa aaacaatact tttattgcaa tatcattaac
     1621 tcttgatagt atacattttg atgcctcatc aattaaatca tcatactttt tattatagac
     1681 ccactgagct cccatagcta atacccaaat aattatagga agatattcaa tactacttcc
     1741 tttaaaaaaa tacttattta ctaatgtagc tccaatacaa taagaaattc ccaatatttc
     1801 tgaaataagt gttgatttaa taccagtttg ttgtttagac atataaaatc ccccttaaaa
     1861 agttatgtat ttataacacc tcttgttata aatatataac ttttttgaat aaatttcaat
     1921 aaaaatatta ttttgcaaca aattctttta ttaattttac attagataga ggcactttag
     1981 atgtcactcc ttgcaatgtc aaatcttcta tattaacttc atttaatttt gaacacagtt
     2041 ctaagtcaaa tattctttcc caacttacct tcttttcaat ttgttcatct tcataaagct
     2101 ctacagtaat atccattaaa ggtaaatgcc ataaatcaaa ttctgataat aacacattgt
     2161 cttcacttat ttctatttca agtaaaacac ctttttttcc tttttctaaa taaccactac
     2221 atcttaaatc aggtctcttc gtccaaaccc atatcatact atcatcaaat tcatatattc
     2281 tatttgtcat ttgactatac atccatttgt atggaatatc aaagtcttct aatataaaag
     2341 aataatcatt tgatagatat ttattgtttt tattgttgtt ccaaaattct aaagtctgta
     2401 tagtataaaa tttcatatat aaatcaaccc ctttgttttt ctataagaag gagatagttc
     2461 aaacttttat tttgaatcat ctccttagct tatatatctc tttgtatcta ctatagttaa
     2521 ataacaaggt tttatattaa tttaaattta aaagaggaag ctttatattt atctagtgcc
     2581 atctcaatta tagtattagt tttgattata gattttttca acctctgatt tattcaaatc
     2641 atctataata tcacatattt cactcaaatt atatccttta ttttttaatt ttatgatgat
     2701 attaaatttc tcttctcttt tccctttttc aacaccttct tgaaaaattt cttctttaaa
     2761 atttctactt ttattcataa tatacgcacc tctctttaaa tttaataaat agtcctcatt
     2821 actgcctaat tcttttaatc tatcaagtgc tcttttaata acataatctg ttttagatag
     2881 tattctaata tcaatatgag aaggattatc aataagttta ccccatatta gaactctgtt
     2941 tgctatacaa ctggatttac gaatttttgg aagttctata aaatataatg cagttttact
     3001 agaataaata tttggatgcc cgtccattgt taatctcagg caatttacat aatcttctat
     3061 gtgattatat tttacatgat ttagtatatg tattccaaca gtttttccaa tttctttata
     3121 ctctttacct tcttctaact ttctaccatg tacacgacta agataatatt cagctctttc
     3181 caaataatca tcttcaagag ctctttgcat ctcaatattt atatgattac tttcttcatc
     3241 ttcagccaaa atataaagtc taggtctttt ctcaccaatg taattaattg ttgggtcggt
     3301 ttcagcctta attattcttt ttatatgagt gaacggtttg atattcctat tggcatatgg
     3361 agcatctttg aggatatcat ttataaaatt tgcaagtaaa tcgtaatcat tatttaaagt
     3421 aaacaatgat ttaaatacct catcatactc aggcttagca tacccatctt cagttacaac
     3481 ttctccaaca ttttcactag tcaatatttc ttttggaatt aaatccttga ttttatctaa
     3541 tttttcatta gaaaaatcac ctctactaag tatgtcattt aattttttgg ttcattctct
     3601 gtccttttct tcaattatag ttcgtttata ctcttttgat gttttaataa tcttagattt
     3661 ttcttctaat ttttctttca tatcaacacc tcttttttat tattacccat ttcttcatac
     3721 caaagtctga acttctaagt aaaatttgca ttattgaaat aagaacctta taaacttata
     3781 tatcattact aaagttaaga ctttaaagag aagcacttag attacaatag attttagata
     3841 gctttctttt taaaactatt gttgataaat gaatgaatat aagggtatgt attaaaatag
     3901 agatactcat ctttttcatt gtttaagtaa tgaaaatata ttttatttaa tacaaaaaag
     3961 agtgccttat aaactaaaaa gttcacttcg agacactccc ttacattttg tttcttaaac
     4021 tttacaatat tacatactta tttattaatt taatctatgc atacttaacc caataataaa
     4081 gtacatatat ccaactcatt aagccatgaa aaatagccca tggaatagat ttccatgcag
     4141 tataagaaat aatcattgct aaacaagaac caaaagaaat acccttttta acagttgatt
     4201 ttatagtatt attatttgaa tccataatta atcctccaaa attcaa
//
LOCUS       DAEPOG010000241         4196 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEPOG010000241 DAEPOG010000000
VERSION     DAEPOG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.243"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(655..906)
                     /locus_tag="KR432_003471"
     CDS             complement(655..906)
                     /locus_tag="KR432_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641976.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1152..1307)
                     /locus_tag="KR432_003472"
     CDS             complement(1152..1307)
                     /locus_tag="KR432_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBH0641977.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   2524..3481
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2524..2552
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         1461 a          627 c          387 g         1721 t
ORIGIN      
        1 cctcttcaat ttgagcccca tcattatata aaattaacct acttgatatc ttaatttaat
       61 atatgtacac tattcatatc ttaatagtca atattattca tttttaatag ctctttttag
      121 atgtcagaat tattatattt tgaaatttat taaatttttg cattaaaaaa actagaaatt
      181 aatttaagtc ttgaattact tataactttc tacgtatcta cctttatatt cttactctga
      241 ttttatatct aatattaact cttcatcaaa gctattaaat atacctctaa ttgagtcaat
      301 tatttcactt acttcacctt taatttttat attaacttca accatttcaa atctcatctt
      361 tttatcttat aatttctata agttttttta tttattgtgt tgcttacttt gaccagcact
      421 tttttattta atagtctaaa tgatattttt taccagtttt aatatcctta aatactatat
      481 ttactaaaaa cttataattt cttttaatac attcttatta ctaataacaa tactcaacaa
      541 ttttatcgcc ttttttcaaa tatgatttaa cttttttatt cctaattatt taaaaatagt
      601 tgtccaattt tcttctatga gaaagtgcta agtattagca acttctctat tctcttaact
      661 ttctactgtc tcaactttct tcatagctat attaatagct acttctttag ctacttcatt
      721 gaactcttcc catcttctcc tattttcttc atcagatata cgtggatata ctatataaac
      781 ttctgtgttt tgattagcta taattttttc atcatattct atattttttt ctttatcata
      841 tttgtatggt ttaaattgtt gtagtatttt cataacatca ccccttgtta taaaatatga
      901 actcataaac ttgtcttata cttatttaat tgttacaatt tttacttaaa atcactttat
      961 attctatatt ttaaagctaa atcctttaca aaagtaacat attcttctat aagtttctta
     1021 tcctcttata tagtatctag tttgtttaac ttatctcttt taaacttaca tacgccttct
     1081 tctgccattc gtcttttatt aattgttaac attgtttcaa gagacacacc aaaactcttg
     1141 ttaagaagct cttagctctc attagtaaaa tattttacat aacttaaatc accttgacct
     1201 ctagcaattt taactgttaa tagacttgtt tcttttttcc aatgtattat atttaatata
     1261 acaacatctt ttattatttt aacttctatt tttacatcta atgccatatt attgctttat
     1321 taatttgtat ccttaaatct taaattttct attaatacat cttctataca gcttagtttc
     1381 tctcgcttaa tcttgaaata tattttctct aaattgtcaa attgttccca agttttatca
     1441 atatctaata ttttgcagtg tctatttgca catctttcga tccaaaggta taattgacta
     1501 gaatgttttg aaatcaactg actattagta ggttagttct taaattcttt caaatactcg
     1561 aattgtaata aaacataatt tacattctct ttatttctat tgaagttagt ttatatattg
     1621 tttacatcat tttcatatat atctgctagt tgctatgcat ttaaaattct cttattattt
     1681 ctttatatta cttgtaaatt attcatcctc atcatcctct taattaaatt tctatcttag
     1741 aaatttaatt tgtaaaaaaa ttttctatat cataatcaag tatcttagat agtttatagg
     1801 taacatatca actctaattg aatattaccc cttcctgtac ttaaaataag ttgatgcatt
     1861 tttaaaacct aactcttttg caacactttg taagtataaa ttttatgtat gtcttctttt
     1921 ttataaaatc atagtgtatc tttttcatat attcacctta attgctatat ttcaataaca
     1981 atatttccaa aacagaaatt tttcgaggta attatgtata gatgtaaatc aaagaattaa
     2041 atatactagg gaaaatatga atagttctca aaaagaattg cttgaaaaaa caaatataaa
     2101 tacaagtgtt atgaatagaa tctggtgaaa gaccaacaag agacgatggg ttaataatat
     2161 ttgctaaaat ttttggttta tatactgatt atgttcttgg tttatcagat acagaaaaac
     2221 taaatattgg caattaaaac tgaattgatt gtattatatg tgtgatgaat ataaatatag
     2281 aattttttga gaagcttgaa atagcttaga aaaaataaga gattcactca agaataagtt
     2341 actaaagatt aaaaaataac aaaagtatct actaaaggat atgaaaatgg tttaagagag
     2401 ttaagtctgg aaatatttat gtgtattctt taaataaaaa tttatttttt aaatgataat
     2461 taaattttta ctgatatggc tatgctttta aaagtcataa ccttattttg atattagatc
     2521 tgtctttaca taccacttag ttaatataaa actaaatata gcttgtaact cctttgatgc
     2581 acttaaaagc tttacatacc acttagttaa tataaaacga atttagctat agaagcatca
     2641 aattgtggca cagcacttta cataccactt agttaatata aaacctttaa agacaaaacg
     2701 tcgtataatt aaatcatagc aactttacat accacttagt taatataaaa ccttataatt
     2761 ctttaagtac tagactgagc aatttaatct ttacatacca cttagttaat ataaaaccat
     2821 cccattcagc aacaacagtc gctaaatctt ctgcctttac ataccactta gttaatataa
     2881 aacttttagt atatttttta ggtctgccct ctttaaaact ctttacatac cacttagtta
     2941 atataaaaca cgaggttata gttaattttg tcgtgggagt ttataacttt acataccact
     3001 tagttaatat aaaacaattt atatatatat atatatatat taaagtcaga cccctttaca
     3061 taccacttag ttaatataaa acttgttgtt cagcattagc aatcatatca tctattcctt
     3121 tctttacata ccacttagtt aatataaaac ctgtaccatc agagacagtc attaaaagat
     3181 ttataaactt tacataccac ttagttaata taaaacttat aaattgtctc tgtacccatt
     3241 ttattcaatg tcgtacttta cataccactt agttaatata aaaccatgaa aaaatgggta
     3301 attcaaactc ttttattttc tttacatacc acttagttaa tataaaacgt tggagaaaat
     3361 aatccttctt atgatttgcc aagcggcttt acataccact tagttaatat aaaaccagcg
     3421 gatgaattat ttatgttaat tggagatgga gactttacat accacttagt taatataaaa
     3481 ccccaaaata aattgagtat ttccaacact tacatctata caattctctc aaatttgcag
     3541 tgaaccatga gtagtgcaaa tgataacatt tatcacatac cctcaatgcc ttatatttca
     3601 actgttaggc cctattttat caaaaatatc gctcactgca aaactattta taattttata
     3661 atattatgaa tatattattt ttaaatatct gtaccaattt gtagtataat aaaatcaaga
     3721 aaataattta ctcctataaa gagtgattat ttttaagtga aaatattatt tttttaaatc
     3781 atccttattg gcgtttggga gatttttatt ttatcataaa tataaactga tataacacca
     3841 gctgacatgc ttgctgcttt cgattattat tcttgctaca attattataa catataaaac
     3901 ttacttattt ttaccaaata ttttatacaa acaataaata ttaacttatg aaatatctac
     3961 tcatttacag atatttcata agttaatagc tctataataa aatttttatc attgtttata
     4021 aaaaatttta ctttgcattt ttttataaac aacagtttta tttataatgt tttgtggtat
     4081 aataaaagca agaagaacta caatctattt tgcgttagag tggagttcat cataaacaga
     4141 gtttattttt tggatttaaa tataaattta aattcaactc gtaagtcact ctttgt
//
LOCUS       DAEPOG010000242         4181 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEPOG010000242 DAEPOG010000000
VERSION     DAEPOG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.244"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(473..868)
                     /locus_tag="KR432_003473"
     CDS             complement(473..868)
                     /locus_tag="KR432_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0641978.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            1009..1401
                     /locus_tag="KR432_003474"
     CDS             1009..1401
                     /locus_tag="KR432_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0641979.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1495..1911)
                     /locus_tag="KR432_003475"
     CDS             complement(1495..1911)
                     /locus_tag="KR432_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0641980.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1926..2837)
                     /locus_tag="KR432_003476"
     CDS             complement(1926..2837)
                     /locus_tag="KR432_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0641981.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(3019..4041)
                     /gene="splB"
                     /locus_tag="KR432_003477"
     CDS             complement(3019..4041)
                     /gene="splB"
                     /locus_tag="KR432_003477"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBH0641982.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1416 a          700 c          452 g         1613 t
ORIGIN      
        1 caaaagttaa tttaaacgat tgactattat ttttcttaac atattcccat aaagaactat
       61 atttactcat attatatcac tccttttaaa attatatctt atctattaat ttatattcta
      121 tcattattaa tgtataaagt catgctactt attatttata tacaaaaata tccaaataat
      181 aagtagcaat tttttatagt cataatattt ttttcagatt aaataaaaat caattatagt
      241 taaaatattt tttaaaagaa atctatccca ttataaagta aattttaatt atcttatttc
      301 tagtatgtaa ttattcaaaa tagtatatca ttattgaaat acacatctaa taactaaaaa
      361 tttttacata ttcattattt aaaaatcttt ttattgcact taaattaaat ctatcaatat
      421 ttcagtaata acgttttctt aattttcact atggatgtcg attcaattat aattaaagct
      481 ctacatcaat ctttttacca ctcattgaac aacaactaac ttttgcctta tcaatagaga
      541 atatttcaaa cttaccatct cccacagagt acatctgacc taaacttggc attatttcaa
      601 gtgccttagc ttgtgtttct tttgatgtaa caaatactgc ttcacctaaa atacgtagag
      661 tattaaggtt ttggtctata caacaaagtt caacctttgg attaaaaata agttgcttaa
      721 acatatcctt ttgattagat gtgcaaaatg taagtttacc attgtattcc ataacaaatc
      781 ctaaaggacg cacatgtgct tgattgttct catcaactgt tgctaaataa taaactcctg
      841 ccttgttcaa aaattctaat gctttcataa ttcttacacc ccttttaaat acttgtatta
      901 aggattatcc tttgatataa ttttaatata aaatacagaa aaaacaagta tgcagttttt
      961 tctgataaag tataaaaaag atactactat aactaaggag taattaaaat gagtataaat
     1021 actgaaaaaa atataactaa aaacgttgaa gaattaacct gtccaatccg ttatgcatta
     1081 gacattgtcg gtggtaagtg gaaattacct attatatgta tgttagcagt tgaaaatcca
     1141 attagatata gtagcataaa aagaaaattg gatggcatta caaatactat gctagctcaa
     1201 tcattaaaag atttagagtc tactggtatt gttcatcgta aacaatataa tgaaattcct
     1261 ccaaaagttg agtatacatt aacaagtaaa ggtaaaagta ttgtgcctat tttacaacaa
     1321 tttgctaatt ggggtgctac taatatgcaa gaaaaaaaca catgtggact tagttgcaaa
     1381 gaatgtagaa agataaagta aatttactaa attattcata ttatagacta tatattaatt
     1441 tatctgattg aatataaaaa caggatgact tatatattca tcctgttcta tatgttaagc
     1501 tctaaattta attagttcaa gcatatcgtt taataccaat aaactatcta cttgaatcat
     1561 tagccattta cttccattaa aaaagtttac ctcattatat atttttttga tataatcaca
     1621 acaagtatta ataaataaat caacctctac tctattgctt tcacttacaa taaccaaagc
     1681 tttaaaataa ccttcttgtg gatataaagt acatagtgat ttacctcttt ttttatattt
     1741 tacattccaa cctctttgca ttgaacattt actgtattca agcttaggtt ctacattata
     1801 agtagaaacc aaagttttat ttaacttgtc ccataatgga ttattaataa attcattaat
     1861 ttgctcaaat gtaggtttag tatcttgaaa aaacgcatct tgccaattca tattcatcat
     1921 cctttctatt gttcaatagg aaccggaaca atccaatgtt ccagatatgc tgcttcaggt
     1981 gtagtggggt aatacatctc aattgcaaaa tcagtagttg ttaaattatg tttcttcatc
     2041 catctttcca taaatttatc tgctttaaaa acagctgaat taactaactc attaaagttt
     2101 tctgcctcaa atccacatac taaatattct ttagctggca actcaaaagt attatatcct
     2161 tctactgatg tacctgactc tacctctaca cctgccatat acatacaatt tccttctttt
     2221 gcatttccca tataaagagc agcaaattca tttccacctg gaatttgatt tggtatatta
     2281 tgtctttgac ttccaagttt catccataat tcctctacta ttgcaattcc agttgtttca
     2341 ccacccacca actggcaaat aggtacttct atttctattc caacaaatgt ccttggatta
     2401 ctcaatgtct tacgattgac ttcaatcact atatcatctg caatcagtgg aacaccttca
     2461 tcaaccatag cataatttaa aattaattct ggcttaataa agtgattgag ttttactgga
     2521 tttgctctat atttttctgg agttaatcca tatgcttttt taaaggaacg tgtaaatgtc
     2581 tcatgactag caaatccaaa atttaaagct acatcaagta tacgattctt atgttctgct
     2641 aagtattcag aagctcttgc aagacgacga agctttatat actccattac agtcttattt
     2701 accaatcttt taaatagtct ttgatagtaa aatggtgata aatttgcttt atttgctaat
     2761 tcattaattg atatttcttc tgataaattt gcttctatta aatccaatgt aatttgaata
     2821 gactcccatg catgcatact ataactcctc ctgatatttt tgttactctt accctttata
     2881 gtatcaaaac tagaaaaaat gttcttgata gatagtgccc tttttaattt tcttatatga
     2941 tagtgctaaa tctcattgct ttattataaa cataaaatat ataaaaataa aatatagcaa
     3001 atctttaggt aagatttgct atattatata ttttattaaa ctatttttaa atactccatt
     3061 tatttcctta agaaagaact ctttcatatc atctaattgt tctttatcat acacgtactt
     3121 accatatcca aattgaccgt atttaaactt tctatcctca ttttccattg gcaatagtgt
     3181 atctggataa atctctagta ttctattttt tgctttagtt gtatatctat gagatataac
     3241 ttcaaatata atttttttat caaaatcctt tggtaaagct tcttttatac ttataatcag
     3301 atttttatac tcttctttcc aaccttcata taaaaacaca ggtgctatta tgaaaccaat
     3361 ctgataacca gccctagcaa gttttacagc tgactctatt cttttatttg cagaggctgt
     3421 ataatgctca tgttcattta taattctagg agtattgata ctaaatcgta tttctgtatg
     3481 attattatgt tcagcatcca ataagctatc cacatcactg tatttagtaa caaatctaaa
     3541 tcttcctttt tcctctcttc caaaaaactc tattgttttc ttcaaagaat tagtatatgg
     3601 ctctactgga actgggtctg atgtagctgc cccttcaaat atggttacct gtggctttct
     3661 atcttcaata tatttttttg tctgttcaag tatttcatct acatttacaa acacccttat
     3721 aaatggttta tcacctaatt gtgtgttcaa ataacaatac tcacatcttc ccatacatcc
     3781 actgacaagt ggtagttgat aattagcaga aggtttacat gtttgaaact tcagactttt
     3841 tctaatccca actaccatag tctgcttccc acttctatat tgttcatata gattttcacc
     3901 tggaatgtgt tctttaatcc tatttgatga tatattaatt atctctacat ctttattatc
     3961 tctaaattta ttgtatattt ttttgcccac atcagtgtct aaagttcctt tttcgaatat
     4021 tattcttttg ggattaaaca tttatttcac ctctacttaa agtttttaaa tcgaagaatt
     4081 ttataaaata ttacttaatt aatcttccct tttttaaact cctttattta ttagaaagta
     4141 aaataaaagc ttaagacaag tttattatat atataacttc t
//
LOCUS       DAEPOG010000243         4159 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEPOG010000243 DAEPOG010000000
VERSION     DAEPOG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.245"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            82..870
                     /locus_tag="KR432_003478"
     CDS             82..870
                     /locus_tag="KR432_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0641983.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            870..1319
                     /locus_tag="KR432_003479"
     CDS             870..1319
                     /locus_tag="KR432_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0641984.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            1323..3587
                     /locus_tag="KR432_003480"
     CDS             1323..3587
                     /locus_tag="KR432_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0641985.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            3727..4038
                     /locus_tag="KR432_003481"
     CDS             3727..4038
                     /locus_tag="KR432_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0641986.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         1618 a          448 c          815 g         1278 t
ORIGIN      
        1 atatttatta tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat
       61 agcaaataag ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta
      121 gaagaagcgt actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct
      181 tttttaagaa tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta
      241 gattatataa aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca
      301 ttagaaacaa gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa
      361 aatataatag gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa
      421 tatggttttt ctgacttgat agtagcatta ctttcattag atacggaagt agaattatat
      481 aatattggga gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt
      541 attaagatat acataaagaa gacaaataag aatgcttctt ataaatcact tagaaatgca
      601 aagagtgatt atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta
      661 tgtgtaggag caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct
      721 aacaatgaaa tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta
      781 acttttggtt ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt
      841 ttaaaaagag ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga
      901 aaaaaagtgt tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta
      961 atttaagtgt gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca gttttaattg
     1021 acaagaaacc tacattatcc tgtgcaacac tgacattgag agctttagat aaagaaataa
     1081 ctacaattga aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag
     1141 gagctgagca atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg
     1201 atgagcttga aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta
     1261 gatgtacagg gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga
     1321 acatgaatat agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa
     1381 agcctgtata tacagatgat ttatcagcaa aaaatgcttt ggtggtaaag atacttagaa
     1441 gtcctcacgc ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg
     1501 gtgttgaatg tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc
     1561 aaacatatcc agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg
     1621 taggtgacga agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga
     1681 atcttatcaa agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag
     1741 gaaataaaac tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa
     1801 gagaaaaaaa tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa
     1861 gtaattgtga tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga
     1921 tggagacgta tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt
     1981 caactcaaat accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta
     2041 gcaaaattag ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcgt
     2101 gtgttgagat tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt
     2161 atgatagaaa agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta
     2221 gactaggtgc tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata
     2281 caggagcata tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac
     2341 caatgtataa taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga
     2401 tgccagcagg agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa
     2461 ctataaatat gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg
     2521 tggtaacaga aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa
     2581 taaaatgtat aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac
     2641 atcttgataa tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta
     2701 tacaaggtct tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc
     2761 ttatgatagg ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg
     2821 ctgaggttct tgatacaagc attgaaaata taacagttat atcagctgat actgacttag
     2881 ttccatatga cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag
     2941 ttaaggcagc tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg
     3001 ttgagaagga tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt
     3061 tattattcga tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc
     3121 ttgcttctca tggtagccca gtatctcctc caccttttat agcaggtttt gcagaaactg
     3181 aaattgacac agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg
     3241 gaacggttat aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga
     3301 ttggaatggc attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata
     3361 catttatgca atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg
     3421 ttgaaactta tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa
     3481 atacaccttt accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata
     3541 cactccctat tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa
     3601 tttagactag ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa
     3661 taagtagtta ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat
     3721 gatgttttgg ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag
     3781 aacattttag aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg
     3841 gtatttttta aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct
     3901 aaattggttg aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata
     3961 gagaaacaac tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat
     4021 ggtagtagag ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt
     4081 tgctgttttt aattaacaat agaaaattta tctttagttt gtatataatt ttaatatcat
     4141 atattctata tatataaaa
//
LOCUS       DAEPOG010000244         4146 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEPOG010000244 DAEPOG010000000
VERSION     DAEPOG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.246"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..885)
                     /locus_tag="KR432_003482"
     CDS             complement(<1..885)
                     /locus_tag="KR432_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0641987.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIE"
     gene            1384..2244
                     /locus_tag="KR432_003483"
     CDS             1384..2244
                     /locus_tag="KR432_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0641988.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(2484..3041)
                     /locus_tag="KR432_003484"
     CDS             complement(2484..3041)
                     /locus_tag="KR432_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0641989.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(3231..4118)
                     /locus_tag="KR432_003485"
     CDS             complement(3231..4118)
                     /locus_tag="KR432_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0641990.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
BASE COUNT         1469 a          570 c          488 g         1619 t
ORIGIN      
        1 ttctatattc tttaacatga ttgaagcata tttatatact ttttcaggtg aataattagt
       61 actactatct gtaagaagct ctgcataccc ttttattggt gaaagtggag tttttaaatc
      121 atgagttata tttgaaatcc actcttctct cataatatca gttttatttt gtatctcatc
      181 acttctatga atttcttcat ttaaaatatt taaactatta tatatatcag caaaaacacc
      241 tttaatatta actggaagat agcttcgttt tgaaatatca ctaacagcag atgtaatatt
      301 tgatatgcac tttgttaccc agtaaccata agcaattcca gaaatcaaaa ttattaaaaa
      361 tataatccct atgattgaaa atacaattga cttaccacct acaaatttac ttccatcaaa
      421 aaacatattc actttagata tatttaatgg aaaatatata atataaatat agtcattttt
      481 attattttta attgtattaa agtatacagt tccattactt gaattatttt ggttagctaa
      541 tgttaataat tcagaattag aataataatc tttatgctct tttggttcta aaaacccata
      601 aactctattt ccactagaat ctaaaatttg caaccacaat tgattttctc gaagcttttc
      661 taatccagac tgttttactt gtggcttatt atctataaat atgatttgtt cattaaagtt
      721 ttctgtaaat tttttcggcc agtcactacg aataaattcc ccatttggtt tctgaatagt
      781 aataagtgat aagaaaatac ctattactgt caggattaag caaataaaaa tcatgaaaaa
      841 aataatgtat gaatgaaata ctgttttata actaggttta tgcatttaat caactctttt
      901 cttaaacttt tattttagtt tgattgttgc ttatataaaa tagataataa ttattgatat
      961 gtaaaaattt atttaaaaat acaactatct acataaatta tatttatata attatatgtt
     1021 atcaagctgt ctgttgtatt taataaatcc ttttgataac atataaaagc aagataattt
     1081 tttccattaa ataccttata attatacaca aaatctctat atacgttaaa cagtatagag
     1141 aaaattttca tagaaccatc tttatagggg atactttata aaagaataaa aaaattataa
     1201 tatataaaaa acttcatagg agtgtaacaa catcagtaaa gatatgaaaa cactatataa
     1261 aaattcaaaa atattaagaa taatgaaata cttgaaattg cttatatcaa gaaattttat
     1321 ataatataaa ctggtcaaat atgttataat attaaattgt gacaagttca tggaggtgtt
     1381 tatatgaaaa aacaaaagtc gccaatttta cttgaaatgc ttggactatt tttgggttgt
     1441 atatcaatga gtattggtat aaatatgttt ttaaaaccac atacaattgc tcctggagga
     1501 cttagtggtc tatcattagt actaaacaag gttacaggac ttccagtatc agctatcatg
     1561 ttaattatag gtgtcccatt agtaatactt gcttttagaa ttatgggtac aaaaaattca
     1621 ttaaaaactt tatttggaac agttgtattt tcagctatag ttcaattaac agacccatta
     1681 tcaaaactaa gatttactaa tgacttatta ttgtcttcta ttgcaggagg aatcctagtt
     1741 ggtataggtt taggaataat gtttaaatca gatgcttcta ctggaggaac agacttaatt
     1801 gcactcatac ttagtaagaa attcccagga ataaaagcta caaaattcat gtcatgccta
     1861 gatggaatgg ttgttatatc ttctggtata gtaaatcgta gtttagaaac tgctctttac
     1921 tctggtatag cactttgtgt attaataaaa atagcagata tgataatgga aggatttgac
     1981 cattcaaaag cattttttat tatctctgat gagcctgaaa gtttaagaca agctataact
     2041 gaagaacttg atagaggtct tactattctt gatggtaaag gtggatacac tagagaaaat
     2101 aaagaagttc tattagtcgt agtttctaaa aaacaagaac tttatctaaa gagattagtt
     2161 aaaagaactg accctactgc atttgttatt gttacagatg tgcatgaggt tcttggagaa
     2221 ggatttaaaa atttagagaa ctaataaaaa ttaatatttt aagtatatat actattttaa
     2281 ttaaaatacg atttattcaa aaagagtgtc tcataaactt tttagttcat gagacactct
     2341 tttttgtata aaatcaaatg cattttcagt attttaacaa taaaaaacag gattatctat
     2401 attttgagtc aactacttta ttgaaatttt atttagaaaa caatcattac taaataacca
     2461 ggatagatat taaatgatta tatctattgt tttttcaatt catattgata acaaggaaca
     2521 ttttttacag tatttccagt atgaattttt ttgatatact ttcctgtaca tttaaatcct
     2581 aataaatcta atgattttgt agaattttgg ttttcttcat aggttattgc aattatttta
     2641 tttaagttta gttctctaaa tccatactgt attactcttt ttgttatctc agtagcaaaa
     2701 tcatgtcccc aatagcttgg atttatacat atacctattt ctgcttctaa tgctttagga
     2761 tttatattat taaaccccat attcccaata actttattgt ctcttttata tactatagca
     2821 aagtggccaa ttttcccttt tttataggga tttagaaaaa atgattttat aaactttctt
     2881 gtatcttcta aagatttatg ctcactaaat ggtaaatgtt ttactactat tttattttta
     2941 taacagtcaa aaaaatcttc aacatcatct aatgatgcag gtcttagtat acaacttcta
     3001 gtttctaagg ttttcatttg aggatattca gaatcagaca tttctaatcc ccctttatac
     3061 attaatctaa atatactgtt catatttaaa atacgatatt aattatataa atgttctcaa
     3121 attaacctta tctttatttt taataaaaac taattactat atgaatcttt caaatttacg
     3181 aatttatctc tatttttata ttaaattact tgtgaaatat gtattctaaa ttatatgtta
     3241 ttagatatca tgcttaaaaa ttctttggtt gctaaggata aagatatatc ttttaaatgg
     3301 cagtaaccta tatttctctt aggtattggc tcttttatat ctaatttaaa caatttctca
     3361 ttttccaaat aatctatgct aaattcttct ataacacaag acactccaag atttatatac
     3421 gcaaattcta aaagtaattc atgagaaccc aattctatat ccgggtttag ttttatacct
     3481 tttgataaaa aatattcatt tacataaagt cttgaatttg ctttattttc tagcattatt
     3541 aaaggcaatg tgtttaattc ttccaatgat attttcctac ctttgtattc tatgtaatca
     3601 tttgcacata caaatatatc atgtatttca atacactcaa caatatttag tgtatcatct
     3661 tctattggca tgtttataat tgctatatct atattcccat ctttgagtaa agatataagt
     3721 tctctactcg ttctatttat tgtagataca tgtatatgtg gatacttctt atgaaaaatt
     3781 tctaaatatt ttaacaagaa aaaacgtgca gctgtatctc ctactcctat tttcagagaa
     3841 ccgtattcta attttttaaa tttatttatt ctatcttctc ctgatgatat caactttaat
     3901 gcagtagtga catgttcact tagaattttc ccctctggag ttaattttac tcccttattg
     3961 tttctataaa ataataaagt atctaattgt tcttctaatt gctttattga ctggcttaca
     4021 gctggctgtg acataaacaa ttccttagca gcaagagaaa aactcttatt atttacaact
     4081 gcattaaaaa ccttataaag ttcaagtttt atattcatat ataattactc cttatgtcaa
     4141 atatta
//
LOCUS       DAEPOG010000245         4138 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEPOG010000245 DAEPOG010000000
VERSION     DAEPOG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.247"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            334..2229
                     /locus_tag="KR432_003486"
     CDS             334..2229
                     /locus_tag="KR432_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBH0641991.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            2691..4094
                     /locus_tag="KR432_003487"
     CDS             2691..4094
                     /locus_tag="KR432_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBH0641992.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         1782 a          428 c          766 g         1162 t
ORIGIN      
        1 atggaggtag atattaattt agtgatatat atttaagtaa taagagcaaa gcaaaaatta
       61 gtgcatagta gtatataaaa atggagtttg aatacttttg ttagtatatg attgtttagt
      121 agaaaatcac tataaaatag ggatttctat ctttgagcat aattatagag agctaagtag
      181 ggggaagggg ttcaagaaga tattattagt gtttgtgtta gtaaattatg taaacacata
      241 aaatatattg aaaaataata ttatatataa tattatttat gtgtgggaaa tacgaatata
      301 aaaaataaaa aataatattc ggaggacata aatatgttaa gcaacaaaaa gagaagtatg
      361 gcaatagtaa tggcaggtgc tacagttatg agtgcagcag caccaatatt cgcagacaat
      421 actgtgacag aaaatgttga taaaaattac acagtaagtg cgaaagattc tgctaaacta
      481 atcgaagagg ttagaaaagc attagaagtt aagtttgaag atacaaaagc aggagcaaat
      541 gtaaatgata gagtttacga tataaaagta gacaatgtaa acttaactaa tgcaactcaa
      601 ttacaaaata aaataaactc tttaacagaa gggcaaagtt taaaagttac tattcaagat
      661 aaaggacatc aagtgttagg tggaaaagta gttgactata aaattgaaaa ctacaaaact
      721 gctcaagaaa tagtagatgc agttaatgct tacaatgcaa ctttagcaga agattctgat
      781 aacaagttaa cagcaactat aaaatctaca aatacagttg aagtaaaaag agcaaaagat
      841 tcagcaaatg ttattacatt aaatgtagga gaccaacatt tagatttctc aaaagtgata
      901 acatcagaag aaggtacttt tgaaggttat gaaaagagtt atagtgacat agattcaaaa
      961 gaattacata cgataacagt taaaaacgca gatttacaag acatatcagc agaagaatta
     1021 tttgatggaa ttagattaac tacattagga agagaaatag ttaataaagt taaaaatgga
     1081 tatgctttaa catttgaaaa tgaagcaatc cttactcaag aacaagaaga tagtgatgat
     1141 aaagataaac cagaaaaatc aagctttgat atagttttaa gcaaggcaaa tgaaaagtca
     1201 gaaacaataa gtgtttcaag taaaaaccat aagttagtta gagacttaca caaagtatta
     1261 actgatgtaa aagatggaaa agaattaaaa gtagaggttt tatctggtga ttcaagattt
     1321 acaacagcag tagaagttag taaagagaga ttcaaagatg gagaagcaga agctataatt
     1381 ttagttggag aagatgctat agttgatgga ttagcatcag caccacttgc ctctcaaaaa
     1441 aatgcaccaa tattattatc taaaaaagat tcactaccat cagaaataga agctgaaata
     1501 ttaagagtac ttggaagtaa tctatcttct aagaaaatat atatagtagg tggagaatct
     1561 aaagtatcaa aagaaactga agaaaaactt tctaaattag gtgtaagtaa agttgagaga
     1621 gtttctggag aagatagatt tgaaacttct ttagaaatag caaaacaatt aaaagataca
     1681 tttaagactg cttttgtagt aggtggaaat ggagaagctg atgctatgag tatatcagct
     1741 agagctgctc aatttggtgc tccaataata gttacaggta acgaattaga tgcaaatgct
     1801 gaaaaattat taaaaggaaa agaattagaa atagtgggtg gagaaaattc tgtatcaaaa
     1861 gaagttgaag acaaattagt tgatatagat ttaaataata aagttgaaag attagctgga
     1921 gaaaatagaa aagatactaa tgctaaagta atcaataaat actatgcagg tgcaactaaa
     1981 gcatatgtag caaaagatgg ttatgtaggt ggaaatggac aattagttga tgcacttaca
     2041 gcagcaccac ttgcagctag ttcaaaggct ccaatagtat taactacaga agaactttct
     2101 aaatcacaag aagaagtagt tgagttaaga cttaagaatg ctactaagtt agtacaaata
     2161 ggtgaaggaa tagctaaaaa cgctattgaa aaaatagcag aaaaaataaa cttatttact
     2221 aagaactaat aagatatagg aattaatatt aaaagtttat ttagataata tatgattata
     2281 caatgcgtta atatgtaaga catagtgtag atattataaa atatacaaat aacaagataa
     2341 aataataaaa ataaggtgtc tcatttagaa ttagattgag ataccttatt ttattttttg
     2401 attataatgt ggagaaattc atattgcaaa aattattata aaaagatact tcttataatg
     2461 attaaacata ttactaacat aaattatttt gaacttataa cttaattaaa taaaataaat
     2521 tataatgttt tgaatttata gagaataaaa actttaggta tattgtttga gttgtatgga
     2581 taactatggt atcatatata aaatatggta acaaaattgt aacttttgat taaaaataag
     2641 tatgagtact aataaaaaat atgaaaagta cattgggggt agaaaataat atgaagaata
     2701 gtaaaaagat attagctata ggacttacac tatttttagt aatggtaaat actcctatgg
     2761 taaatgcgtt gacatcagtt gagcaaataa aagggaatga cagatatgag acagcagcaa
     2821 aaatagcaga taagcaaaac tacaatacag caatactaat caactcagat aatagcttag
     2881 cagatggtct aagtgcaagt ggtttagctg gagccttaaa tgcgcctatt ttaatgacaa
     2941 agcaaaatca gattccaaac acaactatgg aaagattaaa caaagcaaag actgtgtata
     3001 taataggctc agaatcaaca ataagtaaaa atgttgagaa tcagttatta tccaaaaaga
     3061 aggttgtaca aagaatattt ggtgaaaata gatttgatac aagtataaaa atagctgaaa
     3121 aaatcaaaga aattaagcca atagataaag taattatagc caatggattt acaggagaag
     3181 cagatgcgat aagtgcatca ccagtagctg ctagagatgg agtacctata atacttatag
     3241 atggaaatag tgtgggattt gacacgacag gcttaaagag ttatgcgctt ggttctagtg
     3301 aaataatcag cgacgaatta gttaagagca caaattctat tagattgggt ggcacagata
     3361 gatttgaaac taataagata gttatacaag agttttataa aaatagtaaa gaattttatc
     3421 taagtaaagg cttgcaatta acagacgctt tggtcgcctc tacaatagca aaaaatgctc
     3481 ctgtagtttt agttgaaaat ggcagtaaca agagtatttt aagtggtgca gacaaattga
     3541 ctgtactagg aggcataaat cagaatgtta tcaagcaatg tataaatcaa gcttcaccaa
     3601 atcaacaagg gttatattac aatccaaatg atagagcatt taaagagaga ataaagggta
     3661 aagtatatgc tcttacaaag cagtatagaa aagataatgg agtaagagcg ttatctgtag
     3721 caagcagatt agagggtcta gcaaatgatt ggtctaattt aatggcaaat aagaagactt
     3781 tatctcatac gataaatgga aaaaattcat attctacttt tttgaaatac ttagattgga
     3841 gtgagattaa acctggatat atagcagttc aaggtgaaaa tataattaaa tataaaattt
     3901 cagataaacc tgtatataca aatagagatg cagatgatat aggaaacttt atttttaatg
     3961 agtggaagac aaatccagaa gaaggaacta atatgttgca taaagggtat gaaataatgg
     4021 gatttggtat agctataaca ggagataaga atctatatgc tacacatgaa ttttatggaa
     4081 gatataaaga ataagaaaat atacacaaaa ttatgcttgt aaggaagcta tataaata
//
LOCUS       DAEPOG010000246         4122 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEPOG010000246 DAEPOG010000000
VERSION     DAEPOG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.248"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            130..792
                     /locus_tag="KR432_003488"
     CDS             130..792
                     /locus_tag="KR432_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0641993.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            852..1649
                     /locus_tag="KR432_003489"
     CDS             852..1649
                     /locus_tag="KR432_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0641994.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            1782..2957
                     /locus_tag="KR432_003490"
     CDS             1782..2957
                     /locus_tag="KR432_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0641995.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            3066..3815
                     /locus_tag="KR432_003491"
     CDS             3066..3815
                     /locus_tag="KR432_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0641996.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         1657 a          406 c          761 g         1298 t
ORIGIN      
        1 tatcaaaatg gactgtataa tcaagttaaa aaatagtttt tctattgaaa aaataaataa
       61 aattctattg taatttaata ttcgacaaag ttttataaaa aacaacatta agctaggggg
      121 acttatttta tgatttttaa tatgaaatat tttttagaat tatttccaat aataatgaaa
      181 tcgttcaatg taactgtaat tttggcaata agttcattag tattttcatt aattataggt
      241 acaatagtag cattatcatc ttattacaaa gtgaaagtat tgaatcaaat atgtaaagta
      301 tatgtatcta tctttagagg tacgcctctt atgccacagt tattcttttt atattttgga
      361 ctagcgtata tgaacgattt tgttaaaaat atggacccag tatttgcaac atctatagta
      421 cttagtttaa atatgggagc ttatatgtcc gaaacgataa gggcatctat actttcaatt
      481 gataaaggtc agatagaagc agcatatgta atgggattaa caaatttaca aacaatgaaa
      541 aggatagtga taccacaggc cgtaagagtt gcactgccat cattattcaa taattttata
      601 gatttaatta aagggtcttc tgtagctttt gttgttggag taaatgatat aatgggtgct
      661 gccaaatcac aaggagcgtt gagttttaag ttctttgaag tgtatggagc agttatggta
      721 atttactggt gcataataac tcctttaggg tgggggcaaa aaaaattaga aaagaaaata
      781 agcaattatt aagttattaa gaaaatacat aaaagtattt ttatataaaa aatggtgtga
      841 ggagaagaga tatggtaaaa agaaatatta taaaatgttt agtggcagtt ttatcgttaa
      901 catttgtagt tgctggttgt tcaaaaaaag aggaagtaaa ggatgaaaaa gttataactg
      961 tagcttcatc taattcagct gacccatatt cttatgttga taatggtgag cataagggtt
     1021 ttgaagttga tatgtggaaa gagattggta aaagaagtgg atataagata aaaatggaac
     1081 caacaggttt tagttctata tttgggatgt tagattctgg taaagttgat gtggcagcca
     1141 atttctttgg aatgtctaaa gaaagattag aaaaatacga tgcttctata ccttatggtt
     1201 ctgattctgt atgtatagct gtaaaagatg gaaacaacga aattaataaa cttgaagatt
     1261 taaaaggaaa agttgtagca gtttcagaag gttctcaagg gcaacaggtt gcagctagtg
     1321 ttaataaaga taatttgttt gaagaaaagg tttatggtga tggaactaat ggtcttcaag
     1381 atttagattt aggaagagtt agcgcatgga ttgatgcaga acttactgtt gtaggagacg
     1441 ctaaaaaagc taatatgaag gtaagagtat tagatgaaaa attatcagtt actaatatag
     1501 catacttctt taagaaaaat gatgaaaaat ctaaaactat aaaagaagat gtaaataaag
     1561 caatagaaga aatgctttca gatggaacag tgaaaaaaat atctgaaaaa tggtttggaa
     1621 tggatgtaag tgctgatata caaaaataaa tactttaaaa ttaaaatata aaactgtgat
     1681 aactatagat aagtatacta aatttatcta tagttatctg tgtattacaa tgtatttaga
     1741 ttagtcttta taaattaaaa tgagttatgg aggtcttgca aatgattgaa actaaaataa
     1801 aatccattgc actaaaaaat agagaaaaaa tcataaaaat aagacgacag atacacagta
     1861 atccagagtt agcatttaaa gaatttaaaa catccaaact tataaaagaa gagttaaata
     1921 aacttaaaat agaatacata gatgttgctg gtacaggagt attagctact ataaaaggaa
     1981 aaaataatgg tgggaaaact atattgctta gagcggatat ggatgccctt ccaatcaaag
     2041 aggaaaatga tttagaattt aaatcaataa atgataatat gcatgcttgt gggcatgatg
     2101 cccatgtttc atggttgtta ggaacagcaa tgattttaaa tgacataaaa gaagaattga
     2161 atggaaatgt aaaattacta tttcaacctg gcgaagaaaa aggtggttct gatattgtaa
     2221 ttaaagaaaa tgtacttgaa ggtgtggatg cattagcaac tggtcattgt tggccaacta
     2281 ttgaatcagg aaaaattgga atcgctagaa attgtgctat ggcagcgact aatacttttg
     2341 aaataacaat aattggcaag ggcgggcatg gagcagagcc tcataattgt atagacccaa
     2401 ttgcagttgg aaatgcagta tattcatcca ttcaacagat agtttcaaga aaaattgacc
     2461 cagttgtacc tgtagtagtg tctgtttgtt cttttaattc tggtgtttct aaaaatataa
     2521 taccagatgt atgcacactt caagggacta ttagagcgat aagcaaggaa caagtaattg
     2581 aagtaagtaa aattctagaa aatatagtaa gaaatgtatg tagagcatac ggagcagatt
     2641 gcaaatttga aaagagtaca ggaggagatg ctgttataaa tgacaaaaac atgatagagc
     2701 taggaagaga aagtgcttgc aaaatacttg gagttgaaaa tgttgaaata attgattttc
     2761 ctgcaatgac aggtgaggat tttgctattt atatgaaaga aaaacctggt ttatttatgt
     2821 atataggtgt aggaaataaa gaaaaaaata taaactacag attgcatagt aacaagttta
     2881 atatagatga aaaatgtctt agtatagcat cttcattatt ctcacaactt gcagtagatt
     2941 atttatgtca actatagagt acattcataa aaatatattt agcctatttt taaatttaat
     3001 gctagtatat taattttgat gtagaacata ttatttatta tatttattta aaaaggtgga
     3061 taacaatgat aaaagtagaa aatttacata aaagttttaa agggacagaa gttcttaaag
     3121 gtataaattt agagataaat caaagtgaag tagtagctat acttggacca tctggaacag
     3181 gtaaatctac gttacttaga tgtttaaatt atttagttga gcctacaaaa ggaaagataa
     3241 tgattggtga tgtaaaagta gatgtagaaa aagcaacaaa aaaagatata gccaatttaa
     3301 gaaaacatac atctatggta tttcaaaatt ataacttatt taaaaataaa acagcattag
     3361 aaaatgtaat ggaggcattg atagtagtac ataaaaagag taataaagaa gctagagaaa
     3421 tatctttaaa attaatagat aaagtaggta tgttggatag gaaagatttt tatccatcaa
     3481 aattgtctgg ggggcagcaa caaaggatag gtatagcaag agcgatggca gtaaatccaa
     3541 atgtaatatt atttgatgag ccaacatctg cacttgaccc agaacttgtt ggagaagttt
     3601 taagcgttat aaaatcgctt gcgaaagaag gaataacaat gcttattgta actcacgaaa
     3661 taaattttgc aaaagaagtt gctgataaaa ttatatttat ggataatggt gtaatagctg
     3721 aagaaggaac tcctgatgaa atatttaata atcctaaaaa tgaaagaact gcaaaatttt
     3781 taaatatcgt taggaaagaa gaagtattta gttaaaagtg tataaattaa gtattagaag
     3841 atatgtaaga tactcccatt aaatttataa gttagataat aaatttaatg gaagtatctt
     3901 tttattatta gatttattat ttaatttaaa attagaaatg taactatttg aaaattcttt
     3961 ttaaaagtat atcaaaaata tcaagtacat gaaaaaataa tatttgacaa aatatcatat
     4021 atgtttacaa aattaacttg atggaatgat aaagcatagg aattacaatg agtatactaa
     4081 aactttatat taaagtacaa attattaaat ttgaaaataa tt
//
LOCUS       DAEPOG010000247         4017 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEPOG010000247 DAEPOG010000000
VERSION     DAEPOG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.249"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            297..2360
                     /locus_tag="KR432_003492"
     CDS             297..2360
                     /locus_tag="KR432_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0641997.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2577..3143)
                     /locus_tag="KR432_003493"
     CDS             complement(2577..3143)
                     /locus_tag="KR432_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0641998.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            3309..3830
                     /locus_tag="KR432_003494"
     CDS             3309..3830
                     /locus_tag="KR432_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0641999.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT         1738 a          368 c          624 g         1287 t
ORIGIN      
        1 aactagatat ggataatgta aatatacaaa gagaggtatt gaataattat gcaatatctc
       61 tttttaattg ataaataact atgaattact catgaagaag attaaataat aatttttaaa
      121 attaagtatt tgtatatttt atagaataat catgtattta ctttaataaa ataaaaatag
      181 gtataaaaag gtatataata aggctttcat atacatttat gagtcaaaat tttgatataa
      241 tatatagata catggagtta tgaatggaat ttatttaact tataggggga gttaatatga
      301 aagcaggaga aattgaattt cttagttatt tagaggggtc aaacaaaagt tttgtagtgc
      361 ctgtatatca aagaaattat aactggaaga aagaacaatg taaaagattg tttgatgatt
      421 tagaggatat aatagattct ggatttagaa ctcatttctt agggtcagtt gtatctatat
      481 atagtatggg aaaagaatat ttaataatag atggacaaca aagagtgact actgtgtcaa
      541 tattattgat tgccatgtgt caaataataa aagagtttgg agaatcaaaa ggaattatta
      601 caacagaaga acagattaag gagtgttatc ttataaacaa gcactcaaga gataaagatg
      661 gaaaaataaa gttaaaacca atagcagaag atagaaaagc ttactatgaa ctatttgaag
      721 aagaaatgaa tacagataga aattctaata tagtttcaaa ttatttatat ttttataata
      781 gaataaaaga agataatata tcaatagatg atttgtttga tgctatacag aaattaacta
      841 tagtagaaat agagttaaaa aatggtgaag atgaccctca attaatcttt gaaagtctaa
      901 attcaactgg cttagattta agtgaagctg atagagtaag aaactttgtg ttaatgggta
      961 gaagctctaa gttacaagag gaattttata ataattattg gtataaaatt gaaaaaaata
     1021 caagtagcaa cgtaagtagc tttataagag actatttgac tataaaggag agaagtatac
     1081 cgaataaaaa taaagtatat tttagtttta aaaggtatgt atcagataat ggtatagata
     1141 tagaaatttt attaaaagat ttacttaaat tttctaacta ttatagagag atatcttatt
     1201 catgtactga tgataatgat gtaaacaaag ttttgaaaag gataaacaaa cttgaaatat
     1261 ttgtatcata tccgtttata ttagaattat ttaatgatta taatgaaggt ataataaata
     1321 aaaaagtact tgtagaatcc cttaatttag tagaatgttt tatattaaga agaatagtat
     1381 gtgatgtacc tccgaatgca ctgaataaag tatttatgaa cttaggaagg gaaattaaaa
     1441 aatttagtga ttataaagaa aactattcta atatattaag atatattttt ataaataaga
     1501 aatcatctca aagatttcct actgacagtg aattttcaaa tgcttttgaa actaaagata
     1561 tatataatac taagtcaaag aataagttat atatacttga gaggttagag aattataata
     1621 ataaagaaat tgttgattta gaaaatttaa tcaataataa tatgctaaat atagagcata
     1681 taatgccaca gacattaaca aataaatgga aggaatcact aggagataac tataaggaga
     1741 tacatgataa atatttacat acaattggaa atttaacttt gacaggatat aattcaaaac
     1801 taagtaataa aacatttgaa gaaaagaaag aaatggaaga tggatttaaa gatagtaggc
     1861 tttatctaaa taaatatatt tcatctatag ataaatggaa tgaagaagaa ataaaaaata
     1921 gaactaagat attactagat agagctgtag aaatttggaa atatccaagt gttacatatg
     1981 aacctccaag taacagtgaa aatatattta cgttagagga tgatgatgtt aattttacaa
     2041 atgaaagtat agtatcattt agtatattag gagagtcata taaggctaaa aattggacag
     2101 aattttatga acaagttgct cttactttat atgatttgga ttctgtaagg tttaacagta
     2161 ttgtagacaa gacttataaa agagatttta tagataatat taagtctaga gatgtatata
     2221 aattaagaaa aggtattaaa atatcagaaa atatatattt agaaacaaat ttaaatacag
     2281 aggctaaatt aaatataatt agatttttac ttaatgagtt tgatatagag ttaaatgaag
     2341 taatattttc cataaaataa tgaaatatat gcattttttg cgtaatagaa tattaattat
     2401 attaaaaata aaatagcacc tcttgatata gatttatagc tcaaatttat atcaagaggt
     2461 tacgtttaat taaaataata caaatactat ctaattaaat tattaataaa aatgttatat
     2521 aaaaatgagt atatagcatt ttaaagagta ataaatctta cataataaat gatttattaa
     2581 tatgaactag aagtattttt actatattgt tttataaaac ctttaggcat tttacttata
     2641 aaatatacca ccataataca agacaaaggt ttgtctacta tattactaag aatccttgga
     2701 aagaacgcag cagtaaatat cttctgacca gactgaacta aaaaaccaac caataaatca
     2761 gcaccacttc cacttaaacc accaaacaat aaaactgaaa taggagtacc aattaaagga
     2821 caaacaatag ataaaattac tcctgtaata atagcttctt tataaccaaa accttttttc
     2881 ttagaaatat atccaacaat aagaccaaga acaatattta caagagcaaa aggtatattt
     2941 gtaggacctg atataacacc taaaactaag tttgtacacc caccaataag agcaccccaa
     3001 aaaggaccta atacgacagc cccaagtaca gtaccaactg tatctaaaaa taataatggt
     3061 atatttatca tagatataac catacctaag acaatattaa taactatgca tattgaagaa
     3121 aatgtgactt ttttagtatc caattataat acctcctaaa aaaatatata catttatcat
     3181 agtatagatt ataaaagatt actaattgta tatttctata tagaatattg gaaatataga
     3241 acaaataaat aatataatta tcaaaatatg tagtatcatt aaaattaaga ataactaagg
     3301 agattattat gaagagaaat agagagattg tagtagtatg tcattgtata ttaaattgta
     3361 attctaaagt agagggattg tcagaattta agggagcaca aaatatagcg ataaatttaa
     3421 taaataaagg gtatggaata attcaactac catgcccaga aatgattatg tatggaataa
     3481 ataggtgggg acatgtaaaa gaacagtttg acaatttgtt ttttagagaa caatgtaggc
     3541 aaatgttaat acctcatgta aggcagttta atgaatattt aaaaaatgat tatgatataa
     3601 aaagtatcat tgcaatagat ggtagtccaa gttgtggtta taataagaca tgtagttcta
     3661 aacgttggtt tggtgagcta agtggttgca atgaattgat ggataaaata aatgatatac
     3721 aaatggtgga tgggaaaggt atttttatag aagagctaga aaagctactt aaagaatata
     3781 atttagagat acctatagtt ggatttgacg agcttagaga agataagtaa agatataaat
     3841 atttattgtc tcattatgaa acaccattat atttggcaga aaaatatgac tgatggacag
     3901 tacctgaatc atttttaagt ggagtaatta gtacttataa agatgtatct actagctgaa
     3961 agaaaatgta gagaggtaat aaatgaattg ataatatata taaaggagtg tctaaaa
//
LOCUS       DAEPOG010000248         3939 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEPOG010000248 DAEPOG010000000
VERSION     DAEPOG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.250"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..239
                     /locus_tag="KR432_003495"
     CDS             <1..239
                     /locus_tag="KR432_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642000.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="KR432_003496"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="KR432_003496"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0642001.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="KR432_003497"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="KR432_003497"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0642002.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAEPOG010000249         3916 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEPOG010000249 DAEPOG010000000
VERSION     DAEPOG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.251"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            153..1658
                     /locus_tag="KR432_003498"
     CDS             153..1658
                     /locus_tag="KR432_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0642003.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1772..2470
                     /locus_tag="KR432_003499"
     CDS             1772..2470
                     /locus_tag="KR432_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0642004.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2755..3843
                     /locus_tag="KR432_003500"
     CDS             2755..3843
                     /locus_tag="KR432_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642005.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         1539 a          390 c          635 g         1352 t
ORIGIN      
        1 tagaagtaat aaaaaaaaat aattaatata aaaatgtgta caaactaatg aatattgata
       61 tagtaagcta tcatatgaaa gagtatgtac acattttttt tactaatttt tcacaatatg
      121 aacataaata atttacagga aaggaatctt ttatgaaaat tcaaaatact gagtggggat
      181 atattgaatg gaaacatact tatgatgaaa ataatccaaa acaagctatg aatatataca
      241 ttgcagtaac tatgcctggt aagaaacatt ttaatcatgt tcattatggt caagagcaga
      301 tgatatatat tttagaggga gagggtttgt atattataaa tggagtttgg aagccatttt
      361 atcaaggtat gattttttat atagaatctg gtagcacaca tgaaactata aatacaggcg
      421 atagagaaat aaaagaactt attgtttcaa ataatgtaga tgatgtagga gagagtgaag
      481 ttatagatat taatcctaat aattatttaa agaagacctt gataaattat agtgaaagta
      541 cattaaattt gtatgcagca gtagaatcaa ttcgtggtca atttatagac ccgtttaaaa
      601 ttccattaat tatatatgat gattcatgga atatagtttt aaaaaaccct tattttccac
      661 ttttttgttt tgaaaaatgt aatcctatga aatttcctca aaattgtgat tgtatgaatc
      721 aaaaaagtag caatcaattt gtatgtgaat atgggataac tatatataat ataccaatac
      781 tgtacaaaag caatagcata ggtgttataa gaggagggta tgtactatta tctgatttaa
      841 atttagatac agagcataat aatttatatg atattccaga gggagctgct agaagtataa
      901 aacgcctctt gaaacaaata tctaaaaata taataaattt ttgctctttt aatgatataa
      961 gaaaggattt acaagaaaaa gagaagacta tagcaagaac atatcattat ggagagcaac
     1021 ttgaaatgaa tttaaaagta gctcaagata tggttactaa tttaagaata aatcatcact
     1081 ttttatttaa tacattaaat tcaatggcaa gcatagcact agatgatggt agttatgatt
     1141 tgtatagtgc aataatagat ttatcaagaa tgtttagata tacaatgagg tctgatttaa
     1201 gatttgtaga attagaatct gaaatattat acattaaaaa ttatttgaat ttacagaaat
     1261 tacgttatgg agatgcactt aaagtaaaat atcttattcc agagaagtta tataaccttt
     1321 ctgtgccttt taactttatt caacctatcg tagagaatgc atttacacat ggatttagag
     1381 atattgacac agaaaagcga attgaaataa ttgcaagatt agattctcag tatgctatta
     1441 tagagattca taataatgga actatcttag atggaaataa tatagacaaa ataaaggcag
     1501 gtattcgtag taataatggt catggtttgt ctttaatata tacaaaattc acatctgctt
     1561 atggaaataa ttttgatatg gatataaagt cgagtgataa tgaaggaact tacataatga
     1621 ttaaaattcc tattgaaaat tataaggagt atgtatagaa cttgtgaaaa attaataagt
     1681 tttataaatg ttaagaaata tattagatgt ttttatgttg ttttatatat cttacttttt
     1741 tatattatta agctaaaagg gaggtattct catgagaaga gcactaataa tagatgatga
     1801 agaagctgtt ctaaaaataa taacacattt tatagaaatg aaagatatgc caataaaaat
     1861 tgttggtaaa gcaacatctg gtgatgaagc agtagataaa atcattggtt tagagccaga
     1921 catagttttt atagatattc aaatgccaat atacaatggg ttagaagtaa tagaaaaaac
     1981 atctcattta tctaaaaaaa taaactttgt agtaataact gcatttaact attttgaata
     2041 tgctcaaaaa gctttgagat taaatgtaaa agatatactc cttaaaccat tggatatgaa
     2101 agaatttact aagtctgtag aaaaaattat tggatatcaa tataccaata atgatttatt
     2161 aaatgagata ctggaatata taaatctaaa ctatagcaat gacttaaagt taaatgattg
     2221 tgctagatta tttttaacca atccatctaa tatatctagg atttttaaaa gtaatttaaa
     2281 tacaactttt atatcatatc taaatcatat aagaatagaa aaatctattg aattacttga
     2341 aaatactaca aaaagtataa atgagattgc agaaatagtt ggatataata gcctaaataa
     2401 cttttataaa aactttaaga tagaaaaagg aatgactcca aaagtttata aactgaacac
     2461 taaaaattga aattgataaa taaaaagtca atatagataa ttgtttgagt gtgtctgaat
     2521 aagtatactt taattatgat aaaaattaaa gcgctttaaa catatttaat aatattaaac
     2581 cttataaaag ctgtacctat ttatataaaa aaatatatat aaataaaaag aaaaagatat
     2641 acaaaataaa aaaagtgaat taaatactgt ttcaaaaaga cagaattttc atttaattca
     2701 ctgagagttt atatttctta aaggaaggta actttataat ggaggtaatt taatatgtca
     2761 gacaaagtga atatgaaaaa tattatctgt attgcaggcg cttatctagc aacagcaatt
     2821 ggttctgggt ttgctactgg tcaagaaatt ctacaatttt ttgcctcaca agggtatatg
     2881 ggtttaattg gagcattaat atcaatggta ttgttttcat ttcttggtgc tgaagttata
     2941 actagaggta gagaattaaa acttaaagaa ccaacaagaa tttacacttt ttattgtggg
     3001 aagattttag gaaaatttta cgaatggttt agtccaatat ttttatttgg gattcttata
     3061 gtaatgattt caggtgcagg ctcaacttta actgaatact atggtataaa tccttactta
     3121 ggaagagtat tgatggctgt tgctacttta attacagtgt ctatgggttt agaaaaatta
     3181 gtaagcatac ttggacgtat tggaccagtg atagcagtat ttacaattgc tgttggatta
     3241 ataagcttgt ttaagcattt tgatggttta tctatggttg gagaagctat gaaaaatatt
     3301 gaagttacaa aagcatcaga aagtccaatc gtttctggtg taatatataa tacattaaat
     3361 attattgtaa tgattgtttt tttaacagga atgggagctt ctattgaaag taaaaaggat
     3421 gctttttggg gaggtttatt aggtggaata gtgtttatga ctgctggtat agttatgtat
     3481 ttggctatga tttcagatat aggaaatttg tatatgaaag aaataccatc actttattta
     3541 gcagataata tatctcctat tgtaggtgta tgtttttcag ttgcattaat attaggtatc
     3601 tatacaacag ctgttccact tttatggtct gtaaccaata gaattgttga agatgaacat
     3661 cctaaattta aattggtaac aacagtaata gctattttgg cttgtatagg tggtttttta
     3721 ccgtttggta aattagtaaa cattttatat ccatatacag gttatatggg gcttttaatt
     3781 ttagtctgta tggtgtataa agcaattaca tgtagaaaaa aatcaacaca aagtcaggaa
     3841 tagagtcacc tattaatagt ttttatttaa agaaattttt tatataatcc gaatttaaaa
     3901 agtcaaaata gttaaa
//
LOCUS       DAEPOG010000250         3907 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEPOG010000250 DAEPOG010000000
VERSION     DAEPOG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.252"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            142..816
                     /gene="fsa"
                     /locus_tag="KR432_003501"
     CDS             142..816
                     /gene="fsa"
                     /locus_tag="KR432_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0642006.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(938..2332)
                     /locus_tag="KR432_003502"
     CDS             complement(938..2332)
                     /locus_tag="KR432_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH0642007.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2410..3756)
                     /locus_tag="KR432_003503"
     CDS             complement(2410..3756)
                     /locus_tag="KR432_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0642008.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
BASE COUNT         1564 a          537 c          534 g         1272 t
ORIGIN      
        1 atttagtaaa taaaaaaatt ataaaactaa gaaggaaaag caaaacaata ataaaaaata
       61 aaaaacaata agcaaaatgt tgaaaataat accaaatcaa tatataataa gaataaagaa
      121 agattaaagg gggatatttt aatgcaacta atcttagata caggtaatgt ggaggagatt
      181 aaggagttat gtacttgttt accaatagat ggtgtaacta caaatccaag tattgtttca
      241 aaagaaaaaa agaattttaa gcaacttata aatgaaattg gtgagattat aggtgaggat
      301 atgcctatac atgctcaagt gttaagtaca aagtatgaag aaatattaga cgaagcatta
      361 tatataagta gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca agatggttta
      421 agagctataa aagaccttca cagaaaaggt gtaaaaataa ctgctactgc aatattcact
      481 gctcatcaag gttttttagc tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat
      541 aggcttgata atatatctgg tgatggagta gctatggttt cagaacttat aaaaataata
      601 gatacttata aaatggatac aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt
      661 atagaattaa tgcaacatgg ggtgcatagt gttacagtac catatgatat ttgtaaatct
      721 atgatgaatc atccattaac tgattggagt gtagataagt ttatagagga ttgggagaat
      781 acttttggaa aaggaagtaa aacaaataat atttagtatt atggtaaaag agtagttttg
      841 tccaatttca caagaactta tataaaaaga tgaaaatatg aattttcttt ttaataattt
      901 cgttattatc ttaagttaat tcatattttc atctttttta gatttctagt cctataaatt
      961 tccaccaagg gaatgttata catattatta taaagtaggt taaaggaatc aaagttaaac
     1021 cgtatttgaa aataaccttg ctattataca gattttcacc atgaccaacc attattgtag
     1081 catgatgaaa tggtaatatg tactgtatgc ttactataat gtagacaaat agacataaag
     1141 taacactatt ttcctttagt atatgtaaac ttccaagtaa tggtattact acagatgatg
     1201 tagtgacagc acttccaaga catatattca agattattgt gagagttatc aaaaacaata
     1261 ttgaaataat ttctgaagag tttggtataa actttatcaa ataatttccc attatatcta
     1321 caactccaga gtactttaac actcctccaa ttgaaaatgc tgctataaaa aaaataagga
     1381 ccttccaatt tatttctttt aaagtactaa atccaactat ttttcttagt aataaaagaa
     1441 gtacacaaat tgacattata attaatgtgt ttatgttatg tatgaactga gttaaaaaca
     1501 taaaaaacat tattcctagg cataaaaata cgtgtttttg ctctttgttt agcaaattaa
     1561 attctttttt taaattagaa tatgaaaaat gaagtttatt accctcaaga ttttcattag
     1621 aaataaattt tattgaatgc aattcttttt taaagacaaa gataaataaa aaataagtta
     1681 taaaacaagt aattatagtt ggaacagaca tataaaaagc ccattgaccc caatttatat
     1741 tacaatttcc aagttctagt acaacataat ttagaagggt gtctccattt acaaaaaaca
     1801 ttgatgtaaa ggtagatgct gtaaatatac taaataaaag tatagatttt gaatcttcag
     1861 ttatctgttg ttcttttaaa aactccttgt aaaaagcaga tacaagaata acccttggaa
     1921 atggttgtgg gataaaaaat attaaaagaa tacctaaaat atacgataaa agaatcatct
     1981 ttttaggggt atttacagta tttaaaatta acttctcaga aataattttt gcgacacctg
     2041 tttttgtaac tgcattagta attatgtatg caagaattat tatgtaaaaa ttttctgtaa
     2101 ataaaaactg taaaataacc tttattggtg caatgttaaa tataaacgct agaattacaa
     2161 aaaacaatgc aacaaaagtt ttttcaactg agtctacagc ccatatcaag atagttaaaa
     2221 aagataagca tatagaaaat tttatgttga aactgtaatt aagagagaag tagagaatta
     2281 tggtaactat aacagtcata taaaattgtt ttggcttaag aataataccc atttttatat
     2341 tcacctccat aatttattat atcataatgg aggtgaatac aatgaatgtg aatacatttt
     2401 ataaaaagat tattcattga ttctatgatt tacaatagct ttagttttcc aatacccttt
     2461 cttatagtag ataactgtaa caataagacc taaagtccaa ccaaaaggaa tacccatcca
     2521 aattccgtct gttccaaaaa atttagaaag catataagaa gctggaagtc ttccaagcca
     2581 taaagatact attgagctta taagtgcaaa catggaatca ccagcacctt tcattgcact
     2641 tgaaagtaca aaagaagttc caataaataa aaagaatggt cctattataa ataagtaaga
     2701 agagccaatt ttaatgacat ctttatcagt attgaataga gcaattagat tttccctaaa
     2761 tagcaaaatt aaagcaataa ctgtaactgc taaaactaag gtcattttta tagattctcg
     2821 aatgcctttt ttaacccttt cattttctcc agcacctatg ttttgagcaa caaaagtaga
     2881 aacagcagaa ccaagattca taataggtaa gcttataaaa ttttctatcc taagaccagc
     2941 accaaaagct gccatagcag atgtaccata actattaact aaaaattgaa gagtcatatt
     3001 tccaattgaa aacaacattt gttgaattcc acttggaagt cctaatctaa gtccatcttt
     3061 aaatattttg ttatcgtata caattccttt gattttaaat ttaagcactt catgtttttt
     3121 atttaaataa attatactta ttataaatga tactgcctga gccattatag tggcaagtgc
     3181 aacgccctca acacccattc ttagcactac aataaaagtt aagtctaaaa ctatatttag
     3241 aattgtggct attatcagaa aataaagtgg agttactgaa tctccaagtc ctctaagtat
     3301 tgcacttact gaattatatc cagcagaaaa taatattcca ctaaatatta tagtaagata
     3361 caattttgat tgagcaaaaa cggattctgg tgtattcata agcttaagca ttgggccacc
     3421 aaatactatt ccaagaatac ttattaatat agaaccaata aataaaagta tatatgtagt
     3481 atcaatagta gcttttacct tttttaaatc tcctgagcca taaaattgtg acactaaaat
     3541 agaaaatcca aggctaacac ccataagcgc tgccaccaat aaaaacatta tagggttagc
     3601 tccagagact gccgccattg cttcttttcc gataaatcta ccaactatta tactgtcagc
     3661 tgtattgtat agttgttgaa ataaactacc aataagcata ggcatggcaa aaaagaatat
     3721 ggattttccc tcatgacctg tagttaaatc tttcacctta ttcctccaaa taaaaaaatt
     3781 attaatttat tatagtataa tcttaacata aactttatat ccaaactata aataaattgt
     3841 atcaaaatat catatatttt tttaaaatgt aaagaatata attataattt gaatttaata
     3901 aatcaat
//
LOCUS       DAEPOG010000251         3889 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEPOG010000251 DAEPOG010000000
VERSION     DAEPOG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.253"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(123..1946)
                     /locus_tag="KR432_003504"
     CDS             complement(123..1946)
                     /locus_tag="KR432_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642009.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(1959..3734)
                     /locus_tag="KR432_003505"
     CDS             complement(1959..3734)
                     /locus_tag="KR432_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642010.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1289 a          654 c          337 g         1609 t
ORIGIN      
        1 ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat ttattaagtt
       61 agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt ttttctaaat
      121 acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa ttttatcaat
      181 tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat attatcacaa
      241 tttgatatgg tacttaatct atgagctata attatacttg tctttccata agttaaatta
      301 tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt agcctcatcc
      361 aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa ttgtttttga
      421 ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc acgcatttta
      481 actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc atcagtagcc
      541 tccagatttc cataccttat attttccata actgtatcat tgaatatata accttcttgt
      601 ggaactactc caaaacaagt tctcaaatag tttctgttaa tagagtatat atccttacca
      661 tctaaaaata tacttccttc atctatatga taaaaatttg taattagatt tataatcgtt
      721 gtttttccac ttcctgtctc tcctatgatg gcatttgatg tgccagcttt gatttttagg
      781 tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttacatc tttaagctca
      841 atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt aatctcactt
      901 ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa aactctttca
      961 catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt taaaggtctt
     1021 gtgaattgtc ttacataact taaaaaacta gctataaccc ctatggttat aatatcttta
     1081 gatgcaagaa cacctcctat aatacatata gttgcaaatc ctatattact aatcacattc
     1141 ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt tagaagtcta
     1201 tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga ttttactaca
     1261 tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc aacttgttgt
     1321 tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat tactatcggt
     1381 attaatatta cactgcaaaa tgttaaaatt gggcttaata caaacatcat aataaaagtc
     1441 atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat tcctgtactg
     1501 atattatcaa catcatttgt cactctactc ataatatctc cagtagaact tttatcaaaa
     1561 aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc tttaactata
     1621 ctttgagata atctagctgt acatatcccc ttagacagtt taactgaaaa ctcaattata
     1681 taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata agtatcgata
     1741 tctattatgt caactacctt tccaatagta tatggtatta cagttgatat aattgaatca
     1801 attattacta gcaaaaaaat aaatctagta atcttcttat cttttttaaa gtacataaaa
     1861 attcttttaa acgttttttt aaaatcttta ggcttttctt ttttctttaa tctactgacc
     1921 ttttgatttc cagtacttaa gttcaatata cttcctcctt aatttacatc ttcacttact
     1981 aaattctgca acgcaaaaat ttttttataa attggagaac taaccagtaa atcttcatgt
     2041 gtagcaaatc caaccacctc accctcatcc aagactaaaa tcttatcaca gtcaattata
     2101 gaactaatct tttgtgaaat agttattaca gtacatttct tactttcctt ttctcttaaa
     2161 ttacttatat actcttttat attctttctt ataattgtct ctgtattaat atccattgca
     2221 ctaaaacaat catcaagaat taatatttgt ggttttttgg ctatagctct agctatagta
     2281 attctttgtc tctgtcctcc tgataaattt ttaccccttt gaaataaaac ctcatcataa
     2341 gaattttcga gtttagttat aaattgattt gcatttgcta tctcacatga ttttataaca
     2401 tcttctctac ttataccctc atttcccata actatatttt ctagtatact ttttccaaac
     2461 acactgctat tttgaaatac tacacctatt ttttccctta aactttcttc agaaatactg
     2521 tatatactac gtcctccaat cttaatatca cctttatcaa ttatatataa tttttgaatt
     2581 aaatttacta aagaagtctt tcctgaacca gtttgaccaa tcacaccaat attttgacct
     2641 tcttctattt taaaggatac gttttttaat attttttcat ctgtattttt gttatacgaa
     2701 aaatctacat tttcaaaaat tatatcacaa tttacaaact ttaaatcttt ttctccaaca
     2761 tctaaacttt cttctctctc aattacttct tcattctcat caaccaaaac ctcacttatt
     2821 ctctcataag atgcttttgc cctaacaaaa acattaaaaa catgtgaaat cattataaga
     2881 gatgttaata ttctagtcat atagtttaca taagcaacta taatcccaat tttaatttct
     2941 cctgaataaa ttaaatgcct tccaatcagt aatattatta ttatggaaac attaatgatt
     3001 attgtaattg tcggtctaaa aaatgacatc atagttgtag cttttctact atttttaaaa
     3061 agttcatcat ttaactcttc aaagctttgg ttctcataat cgtatctaca aaatagttta
     3121 actactttta ttccacttaa aaactgtctt aattttgaat ttactttatc tgtactttct
     3181 tgtacatttt taaataatct ataccctatc ttcatattaa gcaatattat aataaatata
     3241 attaaaactg caattagaat aattattgac atctttaaat ttaattttat agctataatt
     3301 aaacttccta tacacattat tggtgacttt ataaaaactc tcatcattcc attaacgaat
     3361 tgttgcattt gattgatatc attagtaatt cttgttatta acgaagctct accaaattta
     3421 tctatttgtt ttaatgagta tgtatttatc ttttcaaata catcctgtct taaatcataa
     3481 gaaaaatgct gagaaactct tgccgagatt acacttctta ttattgcaaa tattgcacct
     3541 aaaaaagtta tacctatcat tatagctcct gttgatagta tgtagtctag gttcttattt
     3601 gaaaccccta tatctattat ttttgccatt atagttggtt gagctaaatc acaaaaagct
     3661 tccaatgtta aaaaaaatac tgctgttaga aataatacta tatatttttt tatgtatttt
     3721 ttaaaatact tcattcaatc atcccttcat agaattttct cattaccaaa agtatatcat
     3781 aatggctaat ttaattgatt atatcaatta taaatatttg atttattatt aatttttata
     3841 ccatatattt tataaattat ttgtttttat ttcctatatt tatatatat
//
LOCUS       DAEPOG010000252         3881 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEPOG010000252 DAEPOG010000000
VERSION     DAEPOG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.254"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            698..>3881
                     /locus_tag="KR432_003506"
     CDS             698..>3881
                     /locus_tag="KR432_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH0642011.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRG"
BASE COUNT         1487 a          575 c          655 g         1164 t
ORIGIN      
        1 ttatattgat tattcaaaat tatatataaa attcagaaaa acatttcagt ttacataata
       61 aaaataaagt aaactaaaat tattgatttt agattaaagt gccagattaa gtatttaagt
      121 agattttaag cttgatttga ttagaaaggt acaaactatc accgaaaaaa attaaaccca
      181 tttaaaaagc aatataaaag cttgttattt tcgagaaaaa tagaccctct aaactcaatt
      241 agataaaaat aaaatacaaa aaattatatc tctgaatatc tcaagtaatt aagtttaaat
      301 tttacgatat tttgtttgat gatttataat ataaaagcat attcttaatc ttctcaaatt
      361 aaaaagagta aaaaataaca aaaaaatcac tgacatttca ctgacaaatt gtaattttgt
      421 tattataaat acattgaaaa tacttgatta ttgaaaaatg gcataaaata atacctttta
      481 tgccatttac tattgtttga tagtttttaa cttatgacat aagatatttt ttaacatata
      541 caataaaata taaaaaaatt gatattgcaa ataaaaaaaa tacatattag atgttataga
      601 aaaaaagcgc acttataggc ttaaaatgtc acataaaaga gtttcaaaga ataatatatt
      661 atgaacacaa attttgtaat cacaaaggag tgataatatg gcaacagtat caggacaaat
      721 agtttttgat agaaatagaa gtgctaccat tgattctggg gactcaggaa tagccaatat
      781 accagttgta ttacaagata tagcaactgg agttagactt gtggttctca cagatgcatc
      841 aggaaattat gtttttacta atgttccaaa tggtaactat agaattgtag aagcttttgg
      901 aactacagga ggagtaccaa caccagggaa ttttgcaaca gcgatagtag gtccagttcc
      961 aattgcagaa atgccaccta ttagttctgt gacaaatcct cctattggtt caacaaactt
     1021 agatggtaca acacctaaca cactattaat aacagtagca ggttcagata taataaatca
     1081 aaatatatta aatgggcctg tgatttatac acctatagag aatatattgg attcttgcgt
     1141 gtctgtatct aatataaacc ttattacaga tgcagataac ggaacattcg gtttttttcc
     1201 accaggtaca ccaagtaata caggacctga aatagaacct tacccaaatg tgacgccaga
     1261 ttttacgtat gttgtaccag acccaactaa gtttacacct ttagatggag aatatacagt
     1321 acaaaatatt atgacaaatg caatgagtaa tgttatagga gcttggtggc gtattgctga
     1381 ccatactaca ggaaatgaaa caggaagaat gatggtggta aatggtttta atcctggagc
     1441 tgtatttttt aaatcaacag tacaagttac acctaacact aattatttat ttagcacttg
     1501 gattttaaac ctttttaagg taacaggata tccaccacca cagctaggtg taagaatatt
     1561 tgaccaaaat aataatatac tatatcaagc aacattagga gctcaaattc cagttaatac
     1621 aaacgcacca gaatggaaag aagtaggtac tgtgattaat tctcaaaata ataatcaact
     1681 tacagtagaa ttttttagtg aaggagaaag cgccattgga aatgactatg ctatagatga
     1741 tgtttcttta agagaaatag ctgttccaac ctttactcca ataaaaacaa gtaacacaga
     1801 aactgctaat gtaggtgata taatcacata tacagttaca ttagataata cttgtgatag
     1861 ccctttaact aatgtatttt ttagggattt aattccaaat ggtttagagt ttatacctgg
     1921 aactgtaacg gttgatggag tatctgtacc aggagtagac cctaatacag gttttccact
     1981 tccagatatt ggaggaggaa taggggttga agtaactttt gatgttgtgg ttgaaagtat
     2041 tcctaaccca aatcctacta ataatatagc tactatagat tactcctata cacctgtaga
     2101 aggcggtata ccaaatgatt tttcagtaga ttctaatcca gttcctgttg aagtaatctc
     2161 agcagatgtt gaagttacaa aattatctga acctactata gtgaatccag gagaggaatt
     2221 aatatacact ataaaagtag taaataatgg accattccca tctgagaatg tggttttaac
     2281 agatgatgta ccagcttcta ttataaatcc agagtattca ttagatggag gagtaacttt
     2341 tcaaccatgg actggaagtt taaatatagg gacattagaa gtaggagaga caagagttat
     2401 tataattagg ggtattgtaa atccatcaac agtaggaatt ataacaaata cagctgtagt
     2461 atcttcaact acagcagacc caaaccttaa taataataca tctacaatag agacagaagt
     2521 gaatttatta gcagatattt tagtaatgaa aacagcagaa cctaattcag ctgttccagg
     2581 tactttattg agatatacta ttcaagtaga aaacttagga ccagcaaatg ctgaaaatgt
     2641 aatattaaat gatgatattc cagcctctat tataaatcca gaatattcat tggatggagg
     2701 agcaagtttt caaccatgga atggaagtct taatatagga actttaaact ctggaatttc
     2761 attaacagtt ttaattcaag gaacagttag tttaaattct tcagaatata tagtaaatac
     2821 agcaacagtt tcttcaacta cacctgaccc agatttaagc aacaatatat ctaccattat
     2881 tacaccagtt aacccacaag caggtatttc tattataaaa gtagctgacg aggatgtagc
     2941 tgttccagga gaagaatttg tatatacaat tgaaattttt aatgaaggac catctaatgc
     3001 tacgaatgta gttttaacag atgatatacc agatgttatt ttaaatccag aatattcatt
     3061 ggatggagga gcaactttcc aaccatggaa tggaagtctt aatataggta cagttgcacc
     3121 agggcaatta ataagaatta tcataaaggg attagtaagt tcaactgcta caggagatat
     3181 tactaataca gcagaagtaa gtgcagatgt accagaacca gttacggatt catcaacagt
     3241 gacaactcct atagttccat cagcagatat agaagtaata aaaactagca atatggatac
     3301 agcagttcca ggagaaactt ttagttatac tataacagtt ataaatttag gaccatctgc
     3361 tgcacaaagt atcgttttga cagatgatat accagatgtt attttaaatc cagagtattc
     3421 attggatgga ggagtaactt tccaaccatg gaatggaaat cttagtatag gcactttgga
     3481 tgctggagaa ataagaagta ttataataag aggaacagtg agccagacag cagtaggtac
     3541 aattataaat acagcaacaa taagttcacc tacacctgac ccaaatccag ataataatac
     3601 ttcaacagat gaaacagata taagtccatt agcagatatt tctgtgataa aaggcaacga
     3661 accagttgct ataccagggg gaagatttat ttatggaatt gaaattgcaa atgcaggacc
     3721 atcttttgca gagaacgtaa ctttaacaga taatatacca gcttctattt taaatccaga
     3781 gtactcaata gataatggag tgactttcca accatggaat ggaagcctta atataggtac
     3841 tttagatgct ggagagataa gaaatatcat aataagggga a
//
LOCUS       DAEPOG010000253         3873 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEPOG010000253 DAEPOG010000000
VERSION     DAEPOG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.255"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            208..885
                     /locus_tag="KR432_003507"
     CDS             208..885
                     /locus_tag="KR432_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0642012.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            957..2972
                     /locus_tag="KR432_003508"
     CDS             957..2972
                     /locus_tag="KR432_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0642013.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3022..3702
                     /locus_tag="KR432_003509"
     CDS             3022..3702
                     /locus_tag="KR432_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0642014.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1531 a          386 c          656 g         1300 t
ORIGIN      
        1 cggagacttt aattagtatt taataataaa tcttaatgtt caatacaata ttcaagaatt
       61 gttcaaaaat acttaagtat tatagtctag tttgtaaaga tattataaaa taataatact
      121 agttatatat gaaaataaaa cattataatt tgtaataatt ataaaacttg agattatgat
      181 taaaatacct ttgaatggag tggtgtaatg tataatttac tggttgtaga tgatgatgtt
      241 gatattttaa atataaatca aatttatttt tctaatatag gttttaatgt atatacatca
      301 gagactgcta aagatgctat ggatatagta gagcgtatag ttttagactg tataattttg
      361 gatatttcat tgccagatat agatggatat aaaatgtgca atgatataaa agaaaaggtt
      421 aacataccta taatattttt atctaattat gagcatgaag aagaaaaagt tcggggattt
      481 ttagctgggg gggatgatta tataacaaaa ccttatagtt taaaagaact tgaacttaga
      541 atatatgctc gtatgaggca gtacaaaaat attacatcag ttcctaatgt gttaaagttt
      601 tcagcgttag taataaatgt aaattcaaga aaagtaactt ttgatggaga aagtatagat
      661 ttaactacta tggaatttga aatattattg tttctagctg aaaacaaaga acaagtattt
      721 tcgaagatag agatatataa ccaagtttgg aaaatgcctg atattggaga tcaacataca
      781 gtgcaggttc atattgcaca gatgagaaaa aaaattaatt ctttaagcag ggagcatcaa
      841 tacattcaaa ccgtatgggg aaaaggatat aagtttgtac cataagtgag atactgtaat
      901 taataaatag ttcttgtttt atttaatact actatgaaag agatgtggtt gatgcaatga
      961 aaaagtataa tatactaaaa ggaactctca ttgtattgat agtctctctg ttggcaatag
     1021 caggattaaa tcttctgtat aaatatgata ataaatatac atataagtct attagagcaa
     1081 gtaatggaat tttaactata gataatgata ctttcactaa aggtaaactt gtctttctga
     1141 tagatgactg ggagttttat aatcataaaa tatttaaacc gaatgatttt acagagcaag
     1201 atataaaacc agaatatgtt tttataggtc aatatccaga tttttctatg agaaaaggga
     1261 atcagtcacc atatgggaat gctacttatc gtatgagaat aaaaaatgaa ggtgaggata
     1321 agctactaag tttagagctt cctgagattt tttccgcaag tgaagtatgg ataaatggag
     1381 aaatagtttc taaattagga gatgtaggaa cagaaaaata tagacccaaa attaaaaatt
     1441 cagtagtaag ttttattgca aagaaagata cagaaattat aatcaatgta tcgaattttt
     1501 cacattatta tagtggtttg tattatccgc ctgcattagg agaaacaaag gacataagta
     1561 atatgatgtt ttatcgatta cttttttata gtataatttg cttcacaact tttgcaatag
     1621 ctatattttc gttgtctgta tggtttcttt caaacaaaag taaactttac ttatattttg
     1681 gatgtatgtg tgttttcttt gccatccatg tatcctatcc ctttgtacat ttgttaggac
     1741 tgcctttggt aagctttaca tatgcggttg aagattcgtc ctattttatg gtagttttat
     1801 gtattatttg tataaatgga agtatatcca aaatagaaga agataaagtg tatagatttt
     1861 taattttacc attaggaatt acaatgagta tcatatctat agtgatacca attattcttc
     1921 ttccatatga tatgtacttt gtcaacatat atgggaaatt aatagacata tataaatata
     1981 ttgtatttat atatatatta atgtcttcgc tggtttcaat atataaaaag acatgctctg
     2041 agcatataag ttcttatatt ttagtttcag taaacactgt ttttggaatt agcatattat
     2101 ttgatgcagt tactagtaat agatttgagc ctatatttac agggtggcaa acagaatatt
     2161 gtggatttat aattgttatt ttattttctc ttatgatgat aaacaggaat agaataataa
     2221 taaaagagaa tgaaaaactt acaaggaatc ttgaacatga agttgaagtg agaactaatg
     2281 aacttacaac attgttaaat gaacgtaaaa aattcttggc agatgtggca catgatttaa
     2341 aggcacctgt ttcagctata caatcattta tagacttgat aaaggttggt aatattcatg
     2401 tagatgaaga gacaagaacc tatctttatg ctataaatca aaagtcaaat gaagtacaaa
     2461 atagagtacg tagtttgcaa gaatttacat cacaagataa aagcattggt attagtgaaa
     2521 aaatatgtct aaatgatttt attaaagaga tatatgaaaa taacttgcca gatacacaag
     2581 catgtggtgt aaactttgaa acttacatat tggagaaaaa aatatatgca aagataaata
     2641 aagagatgct tacaagagca tttgagaatt taatttataa tgctttgaca tttactccat
     2701 ttgatggaaa gataatttta tcaatggata aagaagatgg atttgcaatt attaaagttt
     2761 ctgataatgg aaaaggtata cctccagaaa atataccaaa ggtatttgac agattcttta
     2821 cagaaagaga taataatgag ccaaaagggc aagggctagg gttatatatt gtcaaatcca
     2881 ttataagaga gcatggcgga gaaatctttg tagattcaga actaggaaaa ggaactgttt
     2941 ttacaataaa attaagtcta tgttttttat aataatagct tatttaatat aaaagaataa
     3001 tgatatttgg gagtgattat aatgcaaact aagatattga taatagatgg agacaaggat
     3061 aattgccaga agctaaaagg atttctggag gaaaaagaaa taagtataga tttagcatat
     3121 aattgtgaag aagctattgg aaaaatattt agcaataaat atgatttaat atttttagaa
     3181 ataattctaa ctgatggtga tggatggact ctttgtaaaa aaattagaaa tgtaactact
     3241 tgtccaattg tttatatgac ttatataaat gaagaacaaa gcattttaaa tgctttaaat
     3301 tctggcggag atgattactt aataaagcct ctaaatttag aaatattata tgcaaaagta
     3361 aaagctattt taagaagaat gaactcatat gtaaataata atgaaaataa tacaaaatta
     3421 gaggaatata atagaactag tggagtaata aagttagaag acaaaataat aaaattgact
     3481 ccaacagaaa ataagctgtt aaactttttt atagagaatc ctgaaaagac tctgactact
     3541 aaagagatat atgaacatgt atggatgaat gagtacttag aggataacta tagtgtggta
     3601 gttgcagtga atggattaag aaagaaaata gagaaagatt ataaaaatcc tcagaaaatt
     3661 ataacaataa gaggagcagg atattatttt aataaagaat aataaatata ttaatctcat
     3721 taataattgt tataaatgtg aaatgctaac gatatctaaa ctaaattatt ataaaatagc
     3781 tgactgtaat ttaattgata aagcctattc ttctagactt tgccagttaa attacagtca
     3841 gttttattat atgaaaaaat gcgtaacaga act
//
LOCUS       DAEPOG010000254         3873 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEPOG010000254 DAEPOG010000000
VERSION     DAEPOG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.256"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1130
                     /locus_tag="KR432_003510"
     CDS             <1..1130
                     /locus_tag="KR432_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0642015.1"
                     /translation="IFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFKD
                     IKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVNC
                     AAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLLR
                     VLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSLR
                     NRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFRD
                     DLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIEI
                     SESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            1478..1723
                     /locus_tag="KR432_003511"
     CDS             1478..1723
                     /locus_tag="KR432_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0642016.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            1758..2483
                     /locus_tag="KR432_003512"
     CDS             1758..2483
                     /locus_tag="KR432_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0642017.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            2505..3818
                     /locus_tag="KR432_003513"
     CDS             2505..3818
                     /locus_tag="KR432_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0642018.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
BASE COUNT         1509 a          409 c          738 g         1217 t
ORIGIN      
        1 gtattttttc tatggggaat aaagctggga tatattataa cttacaagaa ataacgtata
       61 taaaaaaatt agaacaaaat cttactaata aattaagaga acaaggtcag atagcaagat
      121 atacatttaa agatataaaa actaaaaata aaaatatggt aaagtgtgta gaattggcta
      181 aaaaaatatc taaatcagac ctttctgtat taataatagg agaaagtggt actggtaaag
      241 aattattatc acaatcaatt cataatgctt ccaatagaag taatcaacca tttatagcgg
      301 ttaattgtgc agcagttcca gatagtctat tagaaagtca actgtttgga tatgaaaaag
      361 gctcttttac aggagcttta agagaaggaa aaaagggtct atttgaactt gctaataatg
      421 gaactatatt tctggatgaa attggagata tgcctccatt gctacagaca aagttactaa
      481 gggtattaca agaaaagcag gttatgccta taggttccca taatgttata aacatagatg
      541 ttagggtcat agcagcaaca aacaaaaact tactaaagat gattagagaa ggaaaattta
      601 gagaggacct gtattataga ctaaatgtat tgccaataga tgtaccatca ttgaggaata
      661 gaaaagaaga tataataaca cttatgaatt tctttttgaa aaacaatgta agactttcgc
      721 cagaagttat aactatttta gaaaaatata attggccagg aaatataaga gagttacaaa
      781 atgttgcttc ttatgtaagt ctaatgtgtg atagtatggt ttttagagat gacttaccac
      841 catatataag ggtagaatgt gctaacaatt atgaagatgg atttggttat acaaaaaaag
      901 aatttgagga aatactgagg attctatatg aaagaagaga gtcatcctct ggagttggaa
      961 gagggtttat aatcgaaaag cttatacagg aagggattga gatttctgaa agcaaagtta
     1021 gaaaggtgct atcttattta agtgaatgtg gatatatatc atctaaatct ggtagaggag
     1081 gaagtcaaat aacctcagag gggataaaac tttataataa tataaattaa gctaagtatt
     1141 tgatattgaa atcacatatt ttgaatttag atatttaaat aataaacttt tatatttata
     1201 gagattaatt tatactatgt cagaaagaat attgtaattt ataattaaat attaataggt
     1261 ttttaaaata ggattagata ggtaataggt taagttagaa taacccatta cctattttta
     1321 tttgtaagaa aagtgtacat agagttaaaa gtaaaaaata caactaataa aactattgaa
     1381 aacactagat aaataataaa taaaaataat tataaatttt ggcatatata ttgctaatca
     1441 tagagataag acgacaaaat aattgggagg tttaatgatg aaatacaaaa caacagggac
     1501 ttgtgctaca gaaattgaat ttgaagtaaa agaaaataaa gttacaaatg taaactttat
     1561 aggtggatgt gatggaaatt taaaagggct aaaagtatta gttgaaggca tgaatataga
     1621 agatgtaata caaaagttaa aaggaataga gtgtaaaaca aagccaacat cttgcccaga
     1681 ccaactgtct ctagcattgg aaaattatat gaatataaag taatacataa aacaatcgat
     1741 tagttaggag tttaattatg aaaccaataa tagggatttt aggaaattta ataataatgg
     1801 aaaatggtat gtttccaggt cttgaaaggt catatgtaaa taatgattat ataaatgcag
     1861 tgctaaaagg tggcggtagt ccagtaataa ttcctgtaaa tacagataaa gaagttataa
     1921 aaaaacagat tgaaatggtt gatggagtct taatatctgg gggatgggat atcaatcctc
     1981 aactgtatgg agaagaaaca agagaagaaa cgacatttat atatccagaa gtggatgaat
     2041 ttgatttaat tgcaataagt atagcattag aacttaaaaa gcctatatta ggtgtatgta
     2101 ggggactaca aattttaaat gtaagtttag gaggaacttt atatcaagat aataatctta
     2161 tagaaggaag ttatataaaa catacacaat cttctaaaag acatgtagct actcataaag
     2221 tcgatgttaa agaaggaagt attttagagg gaattttagg aaaacaatta cttacaaata
     2281 gttatcatca tcaaagtgtc aatcagcttg gaaaaggatt aaaagccata gcatactcaa
     2341 aagatggtat aatagaagct attgagaaag aggaggaaaa ttttgtagta ggagtacagt
     2401 ggcatccaga aatgatggta gattactgtg ataaaatgga aaaactattt aaatatttta
     2461 taaatatttg ttcaaattta taatatacct tggggggatt gaacatgaac gaaaagaaca
     2521 aaatgggatt aataagtatt atacttttag gtattaatgc agtagtagga gctggagttt
     2581 ttctattacc aggagatgca atgaaatcat ttggtgtagc aagtatattt gtatatatat
     2641 ttgatatgtt gttagtttta tctatggcat tctgttttgc tgaggttgca ggtaaattca
     2701 ataaaaatgg cgctgcctat gtatatacca aagaagcttt tggagatttt tgtggttttg
     2761 aagtaggtct tatgaaatgg gtaataggat gtatttcttg gggagcttta atagtaggtt
     2821 ttcctacatc attatcagca gtatgggctc cagcaggtga accacatata caaaaaatta
     2881 taattgtagc catgatagtt gggcttacaa ttataaactt attaggtgta tctctatcaa
     2941 aaattgtaca aaatgttata actgtaggta aactaattcc attaatacta tttataggaa
     3001 taggtatatt ttttataaaa ggagttaatt ttacttcttc aacaatggta cctccaggag
     3061 ctggagcaac agagtttgga gcagcagcac ttttaatgtt ttattcattt acaggatttg
     3121 aatctatagc agttgcagca gaagatatgg aaaatccaca aaaaaatata ccaatagcaa
     3181 ttataagtgt aatagtaatt gcatcaataa tatatatatt aaatcaagtt gtatgtgttg
     3241 gtatactagg agattcgtta tctagtacat ctacaccagt ggcagatgct gcaagaatat
     3301 gctttggaaa tatgggagct ggattggtta cctttggaac attggtttct gttggtggaa
     3361 tttgtatgtg tggtgctttt gtaaatccaa gaagttgtgt agctttagct gatgataaaa
     3421 tgttaccaag aatatttgca agaaaagata aaaaaggtac accatatgta gcgataatag
     3481 ctactatgtt aatcactata ccaatagctc tatctggaag ttttgctgaa ttagcagcaa
     3541 taagtgcagt tgctagattc atacaataca taccaacatc tttatccgta ttagtgttta
     3601 gaaagaaaag accagaatta gtaggtactt ttaaaactcc ttttggagca gtgattcctc
     3661 taattgctgt atgtgttgga acatggttgt tattccaagc atctatgcat caattaatta
     3721 tgggattagg agctttggca attggagtac cattatactt tataatgaag tcttataata
     3781 gaaaagtctg tggtgaaaat ttaaagaaaa tagtttaatt atgttataag aatttattga
     3841 gttaaaatta gacattggca gaaaattaaa tac
//
LOCUS       DAEPOG010000255         3734 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEPOG010000255 DAEPOG010000000
VERSION     DAEPOG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.257"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(1..2421)
                     /locus_tag="KR432_003514"
                     /pseudo
     CDS             complement(1..2421)
                     /locus_tag="KR432_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            2587..3330
                     /locus_tag="KR432_003515"
     CDS             2587..3330
                     /locus_tag="KR432_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0642019.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         1259 a          634 c          367 g         1474 t
ORIGIN      
        1 ctaattcttt cttcatatga tttataaact aaagactctt ttattattac tctttcaatc
       61 atacatgaac aatgaaatat aaactttact aaaagttcat cttgaattac taaagaatag
      121 tgttcactaa ttctattaat tacttctttt aatataaaat aagccttttc cccatttaaa
      181 aaagtcaaag ttttactaat attatcaatt agtagtctat caaaatatct accaccatta
      241 tctttttcag catatgtaac ttccatatct ccataatgtt tctttataga ttctataata
      301 ttaaaatata atttctctaa tgtcatttct ggagttagag cctttctagt tgcttctatt
      361 acaataggtg tacttaccat atctactgct tttacttctt ttcctgtagc atcacgaata
      421 actttagcaa atgttaaaat agaccccata tcagttaaaa ttaaaattcc ctttccttta
      481 tcaattttat tggctatttc tacagcttgt actaaaatat cctgaactct atcttctaat
      541 cccatattaa ttgccactgc atggttacaa tccagtaaag tattggctac attcaccatg
      601 ctagatgctg tagattcacc atgcattatg actaatattg ctatactctc ctcatttaat
      661 cccacatacg ataaataaag aaatgttgct aaaaaaattg cttcatcttc tggaaactct
      721 actccataca ttaatgacat gttagataat atttcttttg ctatgttata ttcatatgct
      781 ctttccttca taatttcatc tttattagga tgctttacta tatttccaat ctttattctt
      841 tcaattaaat tctgaatatg taaggctata gcttttatta atttatctaa tccttctctt
      901 ttgctccatt tatcatgctt ttgtaaaata tattttacag tagttgcaat tgaatcatta
      961 acaatgttgt tataagctgt ttgattacta ttattatata taaaagtatt cattaaatta
     1021 taggaatact tctgtatgtc tccatctata acacttctaa tttgtgactc tttaatacct
     1081 tcttgttgaa gtttattcca agtttctttt atcattccat aaaaatctaa atcatatttt
     1141 ttattttcta ttaataaatt ttcactttca aaatcctttt cttctggtaa aaaaataata
     1201 tcatcttttc caataaaact gaaattttgt acaaactcct cttttctatt atttccatat
     1261 aatgattctc taatattatt tggtagaaga gagagcctta catatacaca ttccctttta
     1321 taggtcatat aatctaaaaa agctcttgca caaattaatt gtatatcgct ttttaattgg
     1381 cctatgtttc ccttacactt atataaaata aatgccttaa taacttcttt tgaaaccttt
     1441 aacttaacac caatcctaac actctcttct ttaaaaaaat aacatataag cttcattctc
     1501 tcttgaaagc ctctttcctc taaagacgga atctttatag ttacaggtat tcttcttaaa
     1561 aacgtttcca acattgctgt ttctgggtct tctgtagtgg ctgcaattat cataactcta
     1621 cactcgtgta ccttattagc ctctcctaac cttctatatc cctttatcca taagcagaaa
     1681 tagcatttct tgtccctctg cagataatct atgaacttca tctaaaaaca atactccatt
     1741 attagcttca tgtacaatac catacttatc tttatcagct ccagtaaatg ctccttttac
     1801 atatccaaaa agctgcgcta ataataattg ctgattatca gaataatcag cacagttaaa
     1861 tatgataaat ggtgcattat cactatcttc ttttatagtt aaactatact tatacatata
     1921 ttctgcaaat atagatttac caactcctgt aggtcctatt attaatgtac tcaacccctt
     1981 aggtggatat attactgctg ctttagcctg ttctatttgg gaccttaaac ttccatcaca
     2041 tcctactaaa ttaaaaatat tgttctcttt ttttatactc tctaaaatat actcatcttt
     2101 gatagaatca ttttcactat attcgtttat agagttaata ttatcaaata tccctttatt
     2161 tagtttcttt cccaaaatac tctcaatact ttctttatat aagtactgaa caggcttacc
     2221 ctttatcttt ataataatac tttcttttat taattgattt aattccttac tagcattact
     2281 tctatttata cctacttcag tagctatata ttctgcatca aatccaaatc taatgccatt
     2341 attaaagttc aagtgttctt ctgttaacct tttcaagcaa attaaaactt tttctttcct
     2401 tgatagcttc ttttgtgtca tagttaacct cccctataag cagtaatact gttaaacatt
     2461 tattattaag cttaattcta caatatttta atatttttgt ctaattatgt aactattgat
     2521 tttctttcct attcgtttta taatataaat tacgttaccc tcatctttta tagaggagga
     2581 atttatatga attttttatc agatttacac acccactcta tagtcagtgg tcatggctat
     2641 agcactttac tagaaaatat taattattgt aaagaaaatg gaattaagat actaggtaca
     2701 tctgaacatg gtcctaagat gcctggttct ccccacagat ggtactttca caacattcaa
     2761 aatatcccaa gaatcataaa taacattata attttaagag gctgtgaagc aaatatacta
     2821 gatattaaag gaaacataga cctagagcct tttgtaattc ctcggttaga ttatcttatc
     2881 ttgtcatttc acgaagctgt tttttctcca aatacattag agaataatac taaagcttta
     2941 ataaatgcaa taaataagca tgataatatt gaaatattag gacaccttgg aaacccaaac
     3001 tacccaatcg actatgaatt aatcattaaa ttagctatag aaaagaatat cttaatagaa
     3061 ataaataatt gttctattaa aggcgtgtca agaaatggta gctcggataa ctgtaagtat
     3121 attgctacac tttgcaaaaa atatggtgct aaaatcatac taacttcaga tgctcacatt
     3181 tgttttgaca taggtaacta tgagtattct gagaatatct taaaggaaat taattttcca
     3241 gatgaattaa taatgaatta tcctaaaaaa cttattaatc attttcacca aaaaggtaaa
     3301 ttactagatg ttgattatac aaatatctaa gtaataatac atgcttactt taactcccat
     3361 aaaaatttta atactattta tattatatga aataaagaaa ttataataaa gaagctattt
     3421 aataaagctt aattactttt atgaacttga tggattgata tggtctatga tggtaataga
     3481 aattataaca tttttaatag gcttagtttt atatacctta tagtataaag ctaaaaaaat
     3541 atcatctaaa aataaagtaa atcttatata agatttactt tatttttatt tgtaataatc
     3601 gtcaatacaa attattttat tatatgtaat tatttatatc ttaatcaatt ttattgaaaa
     3661 taacagtctt ctcttttaaa gagatttttc ctacttgata tccatattca tttaattctt
     3721 ttttatattt caaa
//
LOCUS       DAEPOG010000256         3713 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEPOG010000256 DAEPOG010000000
VERSION     DAEPOG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.258"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            186..1562
                     /locus_tag="KR432_003516"
     CDS             186..1562
                     /locus_tag="KR432_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBH0642020.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1873..2544
                     /locus_tag="KR432_003517"
     CDS             1873..2544
                     /locus_tag="KR432_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBH0642021.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2735..3532
                     /locus_tag="KR432_003518"
     CDS             2735..3532
                     /locus_tag="KR432_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBH0642022.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1420 a          402 c          678 g         1213 t
ORIGIN      
        1 aatttaaatt aaaagatata tataaaaatt taaaatattc caaacagaaa ttataaaaat
       61 tactatcatt gtctttatgc aatatttata ctataataga ataataattt agaggaaagc
      121 tctaaaaata aacaaattaa gggtcttaac agtagtgatt ttatgttaag gaggtataaa
      181 gaaagttgaa aacaatagtt agaagactta ttctctacgc agatggaatg cgtcctactc
      241 aaataatggt atctgggttt gctgcgatta ttgtaatcgg agcattgttg ttaacactcc
      301 cgatagcgtc gcagagtgga gagagtatag gcttattaaa tgctctattt acggctactt
      361 cagcggtttg tgttactggt ctagttatgg tagatacagc aacttattgg agtttatttg
      421 gacagatagt tataataaca ttgatacaaa ttggtggtct tggatttatg accgtagcca
      481 caatgttttc acttatggca cgaaaaaaaa tacaactaag agaaagatta ttaatacaag
      541 agtcattaaa tcaagctgat ttatcgggac ttgtaagact tacaagattt gtattaataa
      601 taaccataac aatagaggga ataggtgcac tggttttatc aactgtcttt attccacaat
      661 ttggtttgtc taaagggata tggtatagtg tatttcacgc aatatcagcg ttttgtaatg
      721 ccggatttga cttgatgggc agtgtaagtg gcccatttac atcgttaaat tcttatgtaa
      781 acaactttac agtatcaatg actgtttgcg cacttatagt gcttggaggg ctaggattcc
      841 ctgttgtact tgatattgta agaaaaagaa gattttcaaa attaaatgtg cattctaagg
      901 ttgtattgtt ttcaactgct acacttattt ttgtgggtgc attatttatt tttcttatag
      961 aatttaatca aaaagctact atggcggact tgcctttaaa gggaaaagta ctatcagcaa
     1021 tatttcaatc agtaacagca agaactgctg gttttaatac acttgattta gctactttac
     1081 gagaaagtag tgtatttgta atgataattt tgatgtttat tggagcatca cctgcatcaa
     1141 ctggtggggg tataaaaaca acaacactag ctgttttaat cattacagtt agaagttttc
     1201 tatcaggaaa atcagatata gaagcttttg aaagaagatt agctccatca actataaaaa
     1261 aatctcttgg tatatttgta atcagtatat cagcagttat ttttggaaca ttgattattt
     1321 caataactca gcctaatttt acattagttc aatcagcttt tgaggttaca tcagcacttg
     1381 ctacagttgg ttcaagttta gctggaactc caaatttaaa tgctttaggt aagattataa
     1441 taataatctt tatgtttatg ggtagagttg gttctcttac tttatttatg gctatacttt
     1501 ctggtggtag aagaaagagt caaccaatca gatatgcaga aggtaaaatt atggttggtt
     1561 aagttaccaa ggagtaatat aaatttataa tggatttaga gtagcactgt aaatctgttg
     1621 ataaaataaa tgagcctaga tgtaattata tgaagaaata aaatcaacta ataaacaaaa
     1681 ttaattaatg aaataaaaaa acagggaaat aaaatacaaa taaaaaggta aatcaaaatt
     1741 aaattaatct gtgtatataa aattatatat tgtgtataag ttttgtatgt gtaaagtata
     1801 taagttttgt atgtgtaaac aattaattta agagatatta tataaattgt gtattaggga
     1861 ggaagtttcg agatgaagca atatatagtc attgggtgtg ggagatttgg aagttcagtt
     1921 gcgtctacta tgcatctttt aggacatcaa gtaatggcaa tagacaaaaa tgaagattca
     1981 gttcaaagta tatctgacaa ggtaacccat tcacttatag tggatgttac tgatgagcaa
     2041 gcgttaaggt cattaggttt aggtaacttt gatgtagcag tagttgcaat aggttctgat
     2101 ataagggcat ctataatggc gactcttata gccaaagaaa tgggtgtaga gttgataata
     2161 tgtaaggcaa aggatgaatt acaagctaaa gtgctttata aaattggcgc agatagagtt
     2221 gtatttccag aaagagatat gggagtaaga gttgcacaca atttagtttc ggataatata
     2281 ttagaccata ttgaacttga cccagagtat tcaattgttg aaatcgtaac tccaaatagt
     2341 tgggttggca agacacttat agagcttgaa ttaagagcta gatatgagat aactgtactt
     2401 gctataaaaa caggtaaaaa tataaatgtt acaccttctc cagatgagga acttacagcc
     2461 ggaagtatcc tagttataat cggtcaaaat actagtataa cagcgataac atctggaaat
     2521 aaggggataa ttagaagaag ataatttact atttaatata tatttaattg caatgaaagt
     2581 aaagagtatc atataattat gaatagttat atgatactat ttttatttaa tcgaaggtag
     2641 tatttatttg taagattaga taaagagaag ttaaattaaa agtaaggagg ctgtgctaat
     2701 ataaaaattt ataagttatt agcatttaga taaaatgatt acaaatataa acagtaagga
     2761 taatgaaaag ttaaagtata caagagcact attaaaatca aagaatagga ataaagagtc
     2821 aaagttcata atagaaggat acagaatagt aatgcttgca cttgaatgta tggcaaacct
     2881 tgattatgta tttatcaatg aagaatttga aaataagaaa gaacatgtaa aactattaga
     2941 agatttggat aaaaaaaaca caaagatata caagactact aataaaaact ttaaagaatt
     3001 agtggataca gaaaatactc aaggaataat aggtgtagtt tcatttaaga aaaaaaaatt
     3061 aagtgaaagt ataaataaaa aagataaatt tgtattgatt ttagatagaa tacaagaccc
     3121 aggaaatatg gggactataa taaggactgc tgattctgct ggagtagatg ccataatagc
     3181 actaaaggga tgtgtcgata tatacaaccc aaaagtaatt aggtctacta tgggttctat
     3241 ttttgatatg aatataattg atgcttcaca agatgaaact gtggacatgc ttaaatcatt
     3301 ggattttaat atagtttcaa gttacttaaa tacagaaaat ttttatgaca aaatagatta
     3361 tggttcaaaa gtagcattgg tgataggaaa cgaagcaaat ggaataaatg aagaacttgt
     3421 atcaaagtct gatattttgg ttaagatacc tatatatggt aaagccgagt cgttaaatgc
     3481 tgcgataagc tctgctatac tgatgtatga aataaaaaaa tacttaattt aatgtattgt
     3541 aataaatata atgtcatgtt ataatcttga attaaatagt agagtatcaa aaatagtcaa
     3601 tatatattaa aaaaataatt aaattaatta tatattagat gtatatcata taaaaaatga
     3661 ttgagttgat tatgtattag atatatatta tataaaaaat aattgagtta att
//
LOCUS       DAEPOG010000257         3709 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEPOG010000257 DAEPOG010000000
VERSION     DAEPOG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.259"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            30..1034
                     /locus_tag="KR432_003519"
     CDS             30..1034
                     /locus_tag="KR432_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0642023.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1159..2271
                     /locus_tag="KR432_003520"
     CDS             1159..2271
                     /locus_tag="KR432_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0642024.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2694..3455
                     /locus_tag="KR432_003521"
     CDS             2694..3455
                     /locus_tag="KR432_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642025.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3458..>3709
                     /locus_tag="KR432_003522"
     CDS             3458..>3709
                     /locus_tag="KR432_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642026.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILK"
BASE COUNT         1534 a          368 c          679 g         1128 t
ORIGIN      
        1 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
       61 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
      121 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
      181 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat gcagctttat
      241 tagcattaaa caatgctcaa aagaaactta atatagatac aaacaaagtg gaatcagatg
      301 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
      361 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
      421 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
      481 cttatgaaga taataaatct ggatacctag ctggattaat tgctggaaaa atgactgaaa
      541 gcgataaggt tggttttata ggaggaataa agagttctag tagggataaa tttgaatcag
      601 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
      661 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
      721 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
      781 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
      841 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagtt
      901 ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa cacagttaag tctggaagta
      961 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     1021 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     1081 attggatgtt ataatagaca tatatagaaa gacgtatgca gaggtaggca gacctatcta
     1141 aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt
     1201 tttacaattt ttatattagc tatatgtaac tttgattttg gatttgataa agtaaataag
     1261 aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag tgtaccaact
     1321 tcagcagaaa aggataaatt aaatagtaaa agtaagaatg aagaagatga cacagaagat
     1381 gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa
     1441 tcatataata ataatgtact atcagcctta aaaagggctg atgaagattt tgatgttagg
     1501 ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactacat
     1561 aagtatgaac cagatttgat aatagcagtt ggagctagat ttgcagagcc aattaaagaa
     1621 gctgctacta aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag
     1681 ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt agcagggctt
     1741 attgcaggta aaatgactga tactgggaaa gtcggtttta taggcggagt tgagggttct
     1801 agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct
     1861 gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca
     1921 aaggaaatga gagattctga tagagatgtt gtattttcag ctacagaaga tgatagtaaa
     1981 agagctattc aggtagcaag agaaaatggt ggtaaagtta tagctattaa taaagaccaa
     2041 tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgtc
     2101 tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt
     2161 aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat
     2221 gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg
     2281 tagaagatta taaaaatata ctgtttaagt ttagattagc aataaaaaga tatatttatt
     2341 aaatttgtgc taactgtaca aaatatattg attaagttta gattggtgat aaaaagatat
     2401 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttagat tagcaataaa
     2461 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2521 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2581 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2641 tttatttata ttcatataaa aaaattatca aaaaaaagaa aaaggagtaa aaaatggaat
     2701 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2761 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2821 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2881 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2941 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     3001 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3061 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3121 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3181 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3241 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3301 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3361 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3421 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3481 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3541 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3601 tactaaagct gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3661 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaa
//
LOCUS       DAEPOG010000258         3643 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEPOG010000258 DAEPOG010000000
VERSION     DAEPOG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.260"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            359..2092
                     /locus_tag="KR432_003523"
     CDS             359..2092
                     /locus_tag="KR432_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0642027.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2484..3170
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2484..2512
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT         1461 a          481 c          470 g         1231 t
ORIGIN      
        1 ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt gaattggata
       61 aaaaaatgta taaaaacaag gagaaaaaca agaaaaaatt tgataaaaat gattattaac
      121 attgttattt taaaataaat taaaattatc tttctttttt taaacttgta cattagttta
      181 taaatatgta tctatacaag atattgacat taagtactga agcatatatt gtttcagttc
      241 ttttttgtta aaattaactt atttgacaaa gatatcatag tttaacaaag cttttaattt
      301 aaatatattg atgtataata taattaaaat ataatagcaa aaagaaagga ggaatatcat
      361 gagcatactc ctaaaaaaag cacccaaatt agcaaaacat attataacaa gtttttatat
      421 aaatcgagat atcaatgaag tattaaaata cctttgtgaa aatgttacat ggattggacc
      481 aggagaacag gaatttctca cttcttttaa tgaaataaaa aattattttt atgcaggtca
      541 agatgaaatt ccttcttgtg atataaataa tgatgtattt gaaactgtaa gtgaagatga
      601 gaatagatgt atggtattag gaagatatac tgttcgaact aaagaaaatg ctcaaatgat
      661 tttagaggta aatcaacgtt gtacatttga gataattgag gatagaggaa aattattagt
      721 aaagcacatg cacatatcaa acccttatgg tgaaatgcaa cttgatgaat actttcccac
      781 taaaattgga actcaaagtt atgattattt gcaaagatta ctaaaggaaa aaacagaagt
      841 tattgaaatg attacaaata atatcaatgg tggcttaaaa ggtagcaatg atgatagtac
      901 atactcattt ttctatgtca atgagggatt gcccaaaata ttaggataca catatgatga
      961 gttcatggaa atgagtggtg gttcagcagt gggtgctgta tatccaccag acttacctaa
     1021 agcactagaa gattgtgaac gatgttttgc aaaaggacct acatattcat ctgaatatag
     1081 aatccgaaaa aaagatggaa cattaatgtg ggtattagat tctggaatga aatcgttaaa
     1141 tagtgatggc atagtaaaaa ttaatagtat tattactgac ataacccaac taaaaaacat
     1201 agaatctgaa ctaaaattag aaagagagcg atatcgtatt gctttgcaaa atattacaga
     1261 tatcatgttt gagtatgata ttgaaaacga tacccttata aaatatcaac gtgtagaaat
     1321 tgacaaaaaa attgaattag aaaattttga aacaaaaaat tactccaaac tattagaatc
     1381 tggtaaaatt attcaccttg atgatattgg aaaactacta gaagtacttc atggaaatct
     1441 tcgtgaaaca attgagataa gacaagtaaa ttcattaact aaaaatgaat ggagatggat
     1501 tcgtgttcaa tgttcagtta tatatgatag tgaccataac cctatcaaaa caataggcgt
     1561 tttaaaagac ataactgaag ataaatctaa attagaaagc ttaattaatc aggctcaacg
     1621 tgacccacta acacaattat ataatcaaag agtatcacaa aatcttattg aagagtatct
     1681 atgtagttct gattccaaaa ataatgatgc tctattagtt attgatattg atgattttaa
     1741 aactgtaaat gatacttttg ggcatttaga gggaaatgaa gttttagttg ctgtatcaaa
     1801 aatactatta cataacacac atgataaaga catagttgct cgaattggtg gagatgaatt
     1861 tacaatattt ataaaatcat taacaaagga tttaataata aaaattacta atgatatttt
     1921 aaatgatgca agtaaaataa aagtaaagga caatcataaa ataacattaa gtattggtat
     1981 tgccttcact gatgattcaa ctaaattata taaagattta ttttctaaag ctgataaagc
     2041 tctttatcta tcaaaagcag atggcaaaaa ttgttatagt acatacgaat gataaagcta
     2101 tttatacgac caagaatgaa cctttagatt taatttcata gtctaacaat caaagagttg
     2161 aagctaatct tatagctagt caactagttc ctagaaaaaa tttagacaat atatttattg
     2221 atactgctca tttttctaaa gatcaaattt atataaaaaa ttaaactttt atgtacatac
     2281 agatactaat gataaacatt tagcattagt atcttctatc ttaaaataaa atgataattt
     2341 atctatataa aaaagtgctt tgttgacaga gttcactttt ttatataggt acagaattta
     2401 agtataaata catgttttat tctatacatt ttagttaatg taaaatttct tgattgtact
     2461 ctctaacttt tcaacattat actatttaca taccacttag ttaatataaa accaaaagtt
     2521 ggagaaatag aagtgccatt cacattcgaa tttacatacc acttagttaa tataaaacac
     2581 tttaataatt ctacctttac tagtgctaaa gtaatttaca taccacttag ttaatataaa
     2641 accaggaaca tatggcaaac tataaggaaa acctgcaaat ttacatacca cttagttaat
     2701 ataaaaccct ctgtgactcc atattttttc ataatatata attcatttac ataccactta
     2761 gttaatataa aacatagaag ttgttgcata taactacatt gacaagttat tatttacata
     2821 ccacttagtt aatataaaac ttttctactt ttttaatcat ttaatcacct gcttaaaaat
     2881 ttacatacca cttagttaat ataaaacttt tgatattcta aagaagtgac aatgatgtga
     2941 cacaaattta cataccactt agttaatata aaactattat gtagtcatat atttcaaact
     3001 ctaatttcgt atttacatac cacttagtta atataaaacc catttgaccc atgcatattg
     3061 acttccacct tccttattta cataccactt agttaatata aaactttaca atatagacgt
     3121 acatatactt ctgaaaaacc tctttccata ccacttagtt aatataaaac tttaccccaa
     3181 aataaactgg gcattttcaa tacttacaca tgtgcaactc tcttaaattt gcagtgaacc
     3241 atgagtagtg caaatgataa agtttatcat ataccctcaa caccttatat ttcaagcatt
     3301 aagccctact ttgtaacaaa tatcgctcac tgcaaaacta tttacatttt tattatatca
     3361 taaatatatt atttttgaat atctgtacca atttgtggta taataaaaac aagaagaact
     3421 acaatctatt tagacgtaga gtgaagttca aatactaatg tttattcttt ttgaacttga
     3481 tttttagttc aaaactaaag tcactcctgc tgaactatac ccactgtttt atacagttaa
     3541 aacatgtaaa agtagttata aagtactagt gtaactcatt agtacttttt ttattatatt
     3601 catataatta atatctatat tttaaagttt actatctaaa aaa
//
LOCUS       DAEPOG010000259         3622 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEPOG010000259 DAEPOG010000000
VERSION     DAEPOG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.261"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(65..385)
                     /locus_tag="KR432_003524"
     CDS             complement(65..385)
                     /locus_tag="KR432_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0642028.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(505..1242)
                     /locus_tag="KR432_003525"
     CDS             complement(505..1242)
                     /locus_tag="KR432_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH0642029.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1457..3280
                     /gene="acd"
                     /locus_tag="KR432_003526"
     CDS             1457..3280
                     /gene="acd"
                     /locus_tag="KR432_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBH0642030.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3495..>3622
                     /locus_tag="KR432_003527"
     CDS             3495..>3622
                     /locus_tag="KR432_003527"
                     /inference="COORDINATES: protein motif:HMM:NF025835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642031.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYL"
BASE COUNT         1372 a          462 c          579 g         1209 t
ORIGIN      
        1 agtaaaaaat cattttggga taccatcctt attatttatt ttaataatat ataagtaata
       61 aatcttactt atccatattt tttatgtaac ctcttttttc tagctttttt atatgctcct
      121 tacgtatttc atcaagatta ataccatatt gttcttctag cttaaacatc atagtaacac
      181 atgtctgagc tacatccatt agttccttag cagttttctt tataatatca acttcatcta
      241 aatcattatt ttctccactt agatttctga atttacctat acattcagct aattcacctt
      301 gttcttcacc cattttgata aatgtagatt ctagtgttgg gtctaaatta ttaagctctg
      361 gtaaagatat agtttttagt ttcataaaag ctcctttcaa gttattcgta tttatactaa
      421 aacatcataa caaaaataat actaaaaaaa aactttaaat tcaacataaa aacttacata
      481 tataataaaa atcgtgaaat aaatctatat ttgtcttaat acagcctcat ataaaacttc
      541 tccaataggt ttatttatta caagagaagc tgacttatca tagactgtat ttgctttatt
      601 aataagagca atataatcac ctttgtaata atctataaaa cttgctgctg ggtacacagc
      661 taatgatgta cctcctataa tcaataaatc agcattactt atagcagaaa ttgttttagt
      721 aatgacatca ctgtcaagag cttcctcata aagtactaca tctggtttta ctatagaacc
      781 acagttgtca caataaggaa tattacctcc cagatttaac atagattcta agtcaaaaaa
      841 cttaccacat ttagtacaat aatttctatg tacagaacca tgaagttcta gcacattttt
      901 acttccagcc atttgatgaa gtccatcaat attttgagtt atgacagctt taagcttacc
      961 catttcctcg agttttgcta aagctatatg agcattgttg ggttttgcat taggatatat
     1021 taatttatcc ttataaaatt caaaaaattc ttctggatat ctaacaaaaa aagtatgtga
     1081 aactaattgt tcagcagtaa attgtttatt gagtttttgg ctaaataatc cagtagaact
     1141 tctaaagtct ggaatattag attcagttga aacacctgct ccaccaaaaa atacaatatt
     1201 attgtgatta gctattaagt cttttaagct atttgcatcc atagttacac ctccaaatta
     1261 ttatatataa attgtactac atattaggtc ttttatgttt tattaattta aataaatatg
     1321 tagtttaata agttaaataa agggaatata aataaaatgt atacagagta taaatagaca
     1381 atatttacta aaataacaag ttgtcgtata attatattaa aattgtttta gtgtataaga
     1441 agggcggggg aagtacgtga agaaaaaagc agctttagca acattggcta tgttaccctt
     1501 aggtgtggta aatgcacatg ctgatggaga tataggtata gtgactataa attatttaaa
     1561 tgtaagaaat gagccaactg ccgaaagtag catagccttt gttgctaaaa aagatgataa
     1621 agttttgatt aaagactctt ctaatggatg gtataaaata aaagctgaat ctggacaaga
     1681 aggttgggct tcatcaaaat atatagcaaa atcaaatagt gactctttaa gaacgtctac
     1741 aaataaagaa aaacaagtta tttcaaatag tttaaatatg agaaatggag caggtacaag
     1801 ttacagagta ataactgtac taaaaaaagg tcaaaaagta gaagtaatat cagagagtaa
     1861 tggatggtct aaaatcaaat atgatggaag actaggatat gtatctagct cttatttagg
     1921 agacgtttca aattcaacta ataaatcaaa aactaaacaa gtgaatacaa cttcactaaa
     1981 tgtaaggagt ggaccaaata caagttatgg tttgttaggc aagttgccaa aaggaagtaa
     2041 agtagaagta atatcagaaa gtaatggatg gtccaaaata aagtataatg gaaaagatgc
     2101 ctatatatct agtatgtact tatcggatgt aagtcaaagt aattcagaca attctagtca
     2161 aagcaatgat aaaaagaata ctgataaggt tgtaaataca gcttctttaa atgtaagaag
     2221 tggaccaggt tctacatata gtaagttggg aaaagtttat aaaggaagta aagtaactgt
     2281 actatcagaa agtagtggat gggctaagat taattttaac aataaagaag catttgtagt
     2341 aggcaattat ttatctactt cagcagatac ttcaaataat aactcaaata gtaactctga
     2401 taacagttct aatagtaatg gcaataattc ttcatcatca ggtcaagtaa atggtatgtc
     2461 aggtataagt ggggctaaaa ttgattataa atctttgagt tatactttag aatctcatat
     2521 aagtaaacaa gttgagaaag cagcatcagg aggaaatgtg atagctccaa gtaataggaa
     2581 aagcactcca agtcctgaat ttagtacttt ttcagctcaa agaacaagct catttgtaaa
     2641 tgcaagttca agtgatatag aatattattt aaatcctaaa aattttacaa atactactaa
     2701 aggtatgatg cagtttttaa agattaacag ttatagagat ggtatttcag aatcaagtct
     2761 taactcgtat cttaatggtt tatcttctag tgtatttaaa aaccagggag cagcctttat
     2821 aaatgctgcc aagaagtata atatagatgt tgtatatcta gtatctcatg ctatgtggga
     2881 aactgcttat ggtaagtcta cacttgccca aggtcaaaca ttaacatctt ataagggaca
     2941 acctcttagt aagccagtta aagtatacaa tttttttggt ataggagcaa tagataaaag
     3001 tgctaatgtt tcaggtgcag aagcagcgta ttccaatggc tggactagtg tagaagctac
     3061 aatagatggc tctgcaaagt ggatatcaca aaattacgta aatagttcta agtacaatca
     3121 gaatactatc tacaagatga agtggaatta tgattataca tggcatcagt atgcaactga
     3181 tgtaaactgg gctaatggaa tttctggaat tatggaaaat ttaattggtc tttatggagg
     3241 aggaagtagt ttggtttttg aagttcctca atataagtaa tacataaata aaaaatatag
     3301 actatgggta gttttcattt tatttaaatt atctatagtc tttttgtata taatttataa
     3361 tctgtaaatt taatgttaac aaaactttta tgttataata ttaattctaa gtaaatataa
     3421 gtgagtaata ggtagaaata gataaaaagt ggtaataata tatataaaga taattttgag
     3481 aaaggaggat atatatggaa agtattaagg aatatttgga taagttagaa attaataata
     3541 gtggtcttgg aaagcaactt aaagaagttt atataaatag agtagtttat tttaaggaag
     3601 ataagatagt ttatttttat ct
//
LOCUS       DAEPOG010000260         3597 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEPOG010000260 DAEPOG010000000
VERSION     DAEPOG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.262"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1366
                     /locus_tag="KR432_003528"
     CDS             <1..1366
                     /locus_tag="KR432_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0642032.1"
                     /translation="YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEE
                     SSINSKSDVLVVATKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSV
                     LENLYEFLVDYKKVKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALA
                     TAVAGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSI
                     IKDSTLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVAL
                     GAIIALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLI
                     PSLIEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDT
                     NYISNYNVDKYDEGRSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSI
                     KEAA"
     gene            1581..2438
                     /locus_tag="KR432_003529"
     CDS             1581..2438
                     /locus_tag="KR432_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0642033.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            2535..3404
                     /locus_tag="KR432_003530"
     CDS             2535..3404
                     /locus_tag="KR432_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0642034.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1525 a          326 c          605 g         1141 t
ORIGIN      
        1 ttatatcagt attagaggta tagacataat gaatatttta ttttcattag aaagaataag
       61 tttaatcatg gaaagataca aaaaggatag taaagaattt gaagatgaag atataaaaaa
      121 tattttagaa gaaagtagca taaatagtaa atcagatgtt ctagttgtag caacgaaaga
      181 aataaactat attaagatgt gtttagaaaa taaagatacc ataactgata aagagatatt
      241 aggatttaga gattatgtta ttgctcagga tactaaaaat ataaatttaa aagaaattta
      301 tagtgtttta gaaaatttat atgagttttt agtagattat aaaaaagtaa aatcaagaga
      361 cgaaaagaaa gctgaaagaa aatcaaagat acctcatgaa tttaaacgac tatcaatata
      421 taagaaaaac tttaatttaa attctataag attttcttat gcagtaaaga ttgctttagc
      481 tacagctgta gcgggtttta ttatggatta tttccattta agagatggaa gatggataat
      541 gttaactgtc ttttcattaa cacaacctta cgcagaaaat tgtatacaaa ggtctagaaa
      601 aagaatagaa ggtacattta taggagctgt aatatttatt gtattatttt caataataaa
      661 agatagtaca ttaaggtcgt taatagttct tttagcaggg tatataaact cttatgtagt
      721 tgattataga aaattaatgg tatgtgtaac tgtttcagct cttggttcag cagtagttat
      781 gggagaccca aatgttttaa ccataagcag gatttcatat gtagcattag gagcaattat
      841 tgcattaata gtaaataagt ttatattacc atatgatgct aaaacaggct atcaacatgt
      901 tatagaaatg tataaaggaa tagttaaaaa tataattgat gaagtcaata agtctataga
      961 aaatgtagct gatgtttact atataaaaaa tttattattg ataccatcac ttattgaaga
     1021 tagacttatg ttgataaatg ctatatataa agataaacat caagaggatt ttttagaaaa
     1081 tcagaaatta ctaataagta atatgtataa tctatatata aatgttaata aaaataaaat
     1141 aaaagatgaa gatgttgaaa aaatattgag agatactaat tatatttcaa attacaatgt
     1201 agataagtat gatgaaggaa gaagtgtaat tttagagagt attgtaaata ctaaaagtct
     1261 aggtgacaag ataatatgtc taaacttact gcaaactcta aatggtgtca aagaaatgta
     1321 tagaataagt aatataacta aagtttcaat aaaagaagca gcttagatat taaattatag
     1381 aatagccata tatttaaaat aaagtatatg gctatttgtt ttataaaata ctaaaagaaa
     1441 ataatatata aaaaacaaaa aacatatatt tatgaatttt atatgaaaat agttaaaata
     1501 ttgatttttt tcattggttg aaaaaacaat ttacttagga taaactataa tagtattcaa
     1561 atattatggg ggttagattt atgagtaaag atgtaaaggt aaagcgtaat aagaaatatg
     1621 atacatttag tataactatg gaacttgaga gacctttact ttatgatgag gttgagaaca
     1681 aagatgataa aaataataat agaaaattta acataaagaa taagatattt aagggaatag
     1741 gtatactatt tgtattgatt ttgcttggct ttttcatatg gataaataaa acatatgaac
     1801 ctcaaaagct ggcaaaagaa gctctagtca gcaatagtaa agttgaagta actgtaaatg
     1861 aaaacatttc atttacacca aaaggaagaa aagtttctaa aggtttaatt ttgtatccag
     1921 gagctaaagt agaaatagaa tcttatgctc cacttgcaag aaaaatagca gaaagtggat
     1981 atgaggttgt aatcgtaaag atgccactta atctagctat acttggaact aataaggcac
     2041 agaaagttat ggattcttac aacaacatag aacattgggt aataggaggt cattcattag
     2101 gaggagttgc agcatcaaat tttgcacgtg ataataaatt aattgaaggc gttgtatttt
     2161 tagcttctta tccaatgggt gatgagttga aagaattagg aaaaaaagtt atatctatat
     2221 gggggagcaa agatggagta gtcaatttta aaagtcttgt agaatcaaaa caaaaacttc
     2281 ctgatgatac aacttatgtt gagattgaag gaggaaatca tgcacaattt ggagactatg
     2341 gaaaacaaaa gggagataat gatgctatta taagtcaaga aaaacaactt aatattactg
     2401 ccaatagtat cattaaattt ttaaagaata tatcttaaac taaaatttaa aaaatataat
     2461 tttataagtt attatttaaa gtaaatatta ataatataat agaatgaata ttttattaca
     2521 agggggattt taatatggaa tacgatatgc cactttacag accaccaagt gaggcttata
     2581 gtcttataat acaagtcaca ctaggatgtt ctcataacaa atgtacgttt tgtagtatgt
     2641 acaaatctaa aaaatttaaa ataaagccac tagaagtaat aaaaaatgaa attgatatat
     2701 ttagaagata ttataagcat gtagaaagaa tattcttagc agatggagat gcattgataa
     2761 taccaatgga gaaattaaga gatattattt tatatataaa agaagttttt ccagagtgtg
     2821 aaagaattac tatgtacggc tctcctaaat ctatagagaa aaaatctgat gatgaactta
     2881 aagaattaag aaaacttggt gttaaaatga tttatctagg gcttgaaagt ggaagcaatg
     2941 aagtattaga agatattaag aaaggtttta gtagtgaaca attaataaaa gttggtagaa
     3001 aagttaaaaa aagtgggatt aaattatcag ccactgtaat agcaggtctt ggaggaacta
     3061 aaaaaacaca tcagcatgca gttgatacag gaaaaatgtt aggagcaatt tcaccagatt
     3121 atgtgggtgt acttagttta atggtagaac ctaatactga actttacgat ttacttaagg
     3181 caggagagtt tacaatacta gaggataagg ctgtgttaca agaaattaaa gagatgataa
     3241 agaatataga tgctaatgaa aaaattgtat ttagaagtaa tcatgcatca aattatgcta
     3301 atttaaaagg tgtattacct gatgataaac aacgtctaat tgatgaaatt gattactatc
     3361 taagtaacca aaagctaaaa agagaagaat atagaagact atagtttttg atatatcaat
     3421 ctatattgat aggtaaatac taagattatt tagatacaaa aaatcatgct acattaatag
     3481 ttgcttacta ctgatgtagc atgatttttt tattaaaaaa caaaatggta tattcatata
     3541 taatatatca ataataatga aaatgtgaac tagtttaata gaattttacc tattata
//
LOCUS       DAEPOG010000261         3558 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEPOG010000261 DAEPOG010000000
VERSION     DAEPOG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.263"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(10..1140)
                     /locus_tag="KR432_003531"
     CDS             complement(10..1140)
                     /locus_tag="KR432_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0642035.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            complement(1528..3465)
                     /gene="htpG"
                     /locus_tag="KR432_003532"
     CDS             complement(1528..3465)
                     /gene="htpG"
                     /locus_tag="KR432_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH0642036.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT         1120 a          632 c          351 g         1455 t
ORIGIN      
        1 aaagtcaaat tataaagact tatggtacat ttcctttatt tgttccttat taaaatactt
       61 tggatgtctc ttaatatttg caactgatat tttcatacaa ttatcagtta accaatctat
      121 atctgatttt tcaataccta aatcacttag ttttatatct aaatctattt ttttaaggaa
      181 atttctaatt gcatctgaac aatcttctgc acaagttcct cctaatacct ttgaaatatt
      241 accaaatctt tcaatatcaa tatcccatga actttctata atagtaggag tcaacgctgc
      301 aagtcctttt ccatgcatta catctctaag accacttaca ggatgctcca ttccatgtgg
      361 caaggctgta cccgctgtac caatcgtcat tcctccaata gtacttgcaa gtgttatttt
      421 ttcccatgct tctaaatcat taacatcatt atataccttt attaggtttt ctgatataag
      481 ttcaatccca taaatagctt gtacatctgc aagagggttt cttccattag aaagatatgc
      541 ttccatatta tgggcaagtg catcaaatcc aactgatgca attatatgct ttggcattgt
      601 aaccataagt tctgggtcaa ttattgatgc ttttgcatac atactattct tctttataga
      661 ttttttatct ttagtttctg aatttgtaag tactgcaaaa caatttcctt cactacctgt
      721 acctgatgtt gttggaacta agattatagg taaagcttct tcaccttgtc taattccaaa
      781 tatataatca tttaaatctc ctgggttttt ataagaaaaa gctatactct tggcagcatc
      841 cataatgctt ccaccaccaa gccctaaaac acaatcacat ccagttgatt ttataatttc
      901 tacgcctgaa tatatagtag ttataagagg attttgttct actctatcaa aaacttcata
      961 ttctatttta gagtctttta gtaaatctat tgttttatca agtaatccag ttttctttgt
     1021 actattttta cctgttacta tcaatacttt tttgccatat ttagatactt cttttcccac
     1081 atcattaatt ctgcctctcc caaataagat atttactggc aaattataat taaaattcat
     1141 gctaaaacct cctatttcta ctttagtaat aattttatgt ttttaacaaa gtgtaattat
     1201 gtatttaaac ataaactaaa aaacaatctt ttacttaatt atatgtatta tcttattata
     1261 tatcaagtta actatattta actaaataga gttttttatt agaacctact taaatattta
     1321 aaaattaacc aaagataaaa aaataaagaa cggtctgtct caaaatagag ataaatatat
     1381 ttttcattgt tgaaatgatt aaaataaatt taatttcaca taaaaaaagc gcctcatgaa
     1441 ctgaaaagtt cattttgaga cacttctttc tcatcactga tattgaatac tttttataaa
     1501 tttgcttcaa tgtattcaaa ttcaatctta taattctatc aaaagactca taagcttatt
     1561 acttctttgt acaaatgaat ctaactcatc tggctttaat tctttattag aaagtaaagc
     1621 taaatctgct atctggttac aaataagcgt aatcttatct ttcttttcct catcatcttt
     1681 aagagaaacc aattttttga ttattggact gttctcattt attacaagag ttttttcttc
     1741 ctcaaaattc attcctaaat ccattccagc aaatcttgac tgcatttctg ccattctaat
     1801 agattgttct gatactaata ccatagctgg agtgtcttca tttttcaatc cttctacaga
     1861 ataattcttc actctttctc caacagcttc tttaaacaaa ccttctatct tagctatctc
     1921 ttctttgttt tcttcactat ctttattttc atttttatct tttaatacat cagaaaggtc
     1981 tgcatctatt ctattaaact taactccatt ctctttatat tccataaatg aaataaaatg
     2041 agtatctatt gatgagttta aaacaacagc acttaagtcg tattctttaa atagtttaat
     2101 gtattgagat tgctgttcct tatcacttac ataaaatacc ttattttcat gtttgtcctt
     2161 acatttttct aaataatctt gtattgttat atactcatca tcaatagttt tgaataatat
     2221 acattctttt accttctcat agaaactttc atcttttaag caaccatatt taataaatat
     2281 ttgtatatca tcccaaaact tattgtattc ttctctttca tttttacaaa gagattttaa
     2341 cttatcagct acttttttaa ttatatgttt agaaattttg cttacatccc tgtcattttg
     2401 taagaaactt cttgatacat ttaatggtaa gtctgggcag tctataacac cttttaataa
     2461 aagtaaaaat tctggtataa cttcttttat attgtcagcc acaaacactt gattgttgta
     2521 taattttact tttccttcag tcagttcaaa ctcatttttt aatttaggga agtaaagtat
     2581 accctttaaa ttgaaaggat aatctacatt taaatgtatc caaaataaag gttcatcaaa
     2641 tacattgaat acctttctat aaaactcttt atactcctca tcagtacagt cttttggaga
     2701 ttttaaccat agaggattag tgtcgtttaa aggctcaatt acttttgcat ctacaatctc
     2761 ttcttttcca tcttcctttt gttttttagc tttttcttct gcttctttag cttctctttc
     2821 taatctttca acatcttcta aatatatttc tactggtaag aaagaacagt atttatttat
     2881 tattcctctt acagtaaatt catctaaaaa ttctttacta tcatcatcaa taaatagtgt
     2941 tatagtagtt cctctatcat ttctagcatc tgattcagat atttcgtatt ctgtaccacc
     3001 ttcagatatc catcttacag gagtcgctcc ttctgtatat gaaagtgtat ctatttgtac
     3061 ctttttagac accataaatg cagaataaaa tcctaatcca aaatgaccta ttatgtcatt
     3121 tgactcttcc atcttatcct tatatttatt aaagaaatct tcagcacctg aaaaagcaac
     3181 ttgatttatg tattttttta tctcttcttc agtcatacca attccattgt caataaattt
     3241 aagtgtacct tctcctttat ttacagatac tgtaatttta taatctgatg acttattttc
     3301 tgatatttct cctagtgata caagtctttt gtgtttgctt actgcatcac atccattact
     3361 tataagctct cttataaata tgtctttatc agagtataac cattttttaa ttataggaaa
     3421 aatattttct gtatgaattg aaatactccc tttttcaaat tccatttttt atccctcctc
     3481 attttataat taatatttta aattttttaa aatggtatgt caatatatta taatcatatt
     3541 attttaaaat aaaataat
//
LOCUS       DAEPOG010000262         3464 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEPOG010000262 DAEPOG010000000
VERSION     DAEPOG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.264"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1170
                     /locus_tag="KR432_003533"
     CDS             <1..1170
                     /locus_tag="KR432_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH0642037.1"
                     /translation="PIICHYNLSKKVLDDILLKNNISIIISDESMDVIDLYDDNNNEV
                     AKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFF
                     PIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQY
                     NVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTARNLKKYMPKSEIILYYG
                     ASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNTEYAVYNATKPYSVNDI
                     GYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIEDAIVLPYSDTIRGNQI
                     VLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1246..2121
                     /gene="dpsA"
                     /locus_tag="KR432_003534"
     CDS             1246..2121
                     /gene="dpsA"
                     /locus_tag="KR432_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBH0642038.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            2124..2714
                     /locus_tag="KR432_003535"
     CDS             2124..2714
                     /locus_tag="KR432_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBH0642039.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2922..3122)
                     /locus_tag="KR432_003536"
     CDS             complement(2922..3122)
                     /locus_tag="KR432_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642040.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         1327 a          414 c          511 g         1212 t
ORIGIN      
        1 cctattattt gtcattataa tctttctaaa aaagtattag atgatatttt attaaaaaat
       61 aatatttcaa tcattatatc tgatgaatct atggacgtta ttgatttata tgatgataac
      121 aataatgaag tagcaaaaaa agttaatttt tctattttta atcctaaatt taatctatat
      181 atgtatcaat ataagaacct tataaattat ttagataaaa gtatttgtat gggagcttta
      241 tcttcaggtt ctacaagtgt tccaaaggta ttatatagaa catatgagag ttgggctgga
      301 ttttttccta tacaaaatgg tatttttaat atctctgggg aatcaatctt gtttgttaat
      361 ggaacactta gttttacagg gaatctaaat tctataatgt cagtattata tgaaggaggt
      421 agtattgtaa taagttcagg tcttaattgt aaatcatgga taaggacaat aaaacaatat
      481 aatgtaacta acatatacct tatacctaca aaacttcaac ttttggttaa acacttaaaa
      541 gaaccagtac tcaaagtagt aagtattttt acagggtctc aattattatt tgaatatact
      601 gcaagaaatc taaaaaaata tatgccaaag tctgaaatta ttttatacta tggggcaagt
      661 gaattaaatt atattacata tttatgctat gatgaactaa ttgaaaaacc tctaagtgta
      721 ggcagaccat ttccaggaat agatatatat attagaggag gaaaaatttt tgtgaataca
      781 gaatatgcag tatataatgc aaccaaacct tattctgtca atgatattgg atattatgat
      841 aatgatggat acttaatatt tgaaggaaga agtgatgaca ttgtaaatat tggaggattt
      901 aaagttagtt ctgctaagat tgaaaatgaa gttaaaaaaa ttcctcaaat tgaagatgca
      961 attgtattgc cgtactcaga cactataaga ggaaatcaaa ttgtattatt tgtaataact
     1021 atagacaaaa ttacgaagaa agatttgttg attaaaatga aagataatct aatgaaaaat
     1081 gaaatcccaa agaaaattat ctttttaaat tcatttccat atacctcttc tgaaaagata
     1141 gatagattag cattattaaa aatgttataa gtataatatt tagtaatttt agtgaaccaa
     1201 tgtattgata aaaacatatt gtatatagta aaggaggctt acaatatgtc aaatcaatac
     1261 gatataactg ttattggggg agacctgcga caagtgtaca tggtaaaaaa actaattaat
     1321 aaagggtatt ctgtagtcac ttatgggata tcaaatgaaa ttataaatgg aatcgttgga
     1381 aaatcaactt cactagctga agcactatcc aaaagttcca ttattttatg tccaattcca
     1441 tttacaaaga atcaagttaa tattgaaaat acggatgatt gtcttgatgc tactattgat
     1501 aacttgctaa ttaatttatc tcctaatcaa atattatttg gaggtaacat accaactaaa
     1561 gtttgtaaat tttgcgagca acactctatt cagatttatg attttatgaa aatgaatgat
     1621 gttgctattt taaatgcagt tgctacagct gaaggggcta tagcagaagc tattaaaaga
     1681 agtataatca atatacatca aagtaactgt ttagtattag gttatggaag atgtgcacaa
     1741 atacttgcaa aaaaattata cgctttggat gcaaatgtat gcgttggtgc aagaaataat
     1801 gatgcaagaa gtacggctaa tgcatttgga tattccagta tttctttaag tcaacttgat
     1861 gaaaaacttt tacaatttga ttatattttt aacaccatac cagagtgtat tttaacgaaa
     1921 gaaagattag aaaaagtatc acaagaagta actattatag atatagcttc ttctcctgga
     1981 ggagtagatt attctgttgt taaagaagta gggattaatg cgactctttg ccttgggctt
     2041 cctggtaaat attcaccaaa gacatctggt gagattcttg ttaacgctat agaaaatatt
     2101 ataaatgaaa gaagggatta gaaatgagac ttgaaggact tacaattgga gtaggattta
     2161 ctggttcgtt ttgtacttat gataaaatat ttatagagtt agagaatttg gttaaagaag
     2221 gagctaatgt acacactata ttttcagatg tatcacaaaa tatcgattgt agatttggaa
     2281 attctgaaga gtttatgaaa aaagcatatg aacttacagg aaataaacct atagttacga
     2341 ttgaagaggc agaaccattt ggacctaaag ggattgctga tattataata atagctcctt
     2401 gtactggaaa tactgctgca aaattagcaa atggtattac agattcacca gtattaatgg
     2461 cagcaaaagg tcatcttcgt aatgataaac cactagtaat atcaatatca acgaatgatg
     2521 ctctaagctt taatttcaaa aatattggta ttctccttaa ttctaaaaat atatattttg
     2581 taccttttgg tcaagataat tgcaaagcaa agcctaattc tatgattgca catactgagc
     2641 taataattcc aactatagaa cttgctctag agaacaaaca gctacagcct gttataaaaa
     2701 gtccacatca atagaattgg ttcttataat attatgtatt agatacaata ctagcaaaga
     2761 ggctgtctca aaatagagag aagcctcttt ttcattgttg aaatgatgaa aatatattta
     2821 atttcataca aaaaaatgca tcatgaacta aaaagtctat tttgagctaa tctccgtaat
     2881 ttttggtata ttaaaattat ttacttaatc caatttttaa attagaattt atatcgtttt
     2941 gactcttcaa atatcaatct aactgttgtt gatacaaaag aagaggacat aattaaaaca
     3001 aatactgata gtcctctaat aaacaaatta tctacataat aagttactat tccaatacct
     3061 ccaatcgtta aaattacact tataatcaaa gaaaaaatgc taattgtttt taattttttc
     3121 atgcttatcc tccttcttat aaacttcaaa tttatctata taatattaaa tatattttat
     3181 ttcatagtat ataaaaatca tgaatatata tttttttaat tatactatat gttgtaaatg
     3241 tttgtacatt taactatatt tactttaact tgttaatcaa gttattattt tcattttcaa
     3301 gtatttttat ttttaaactt gatagaatat catattaaaa attcttttta taaagtttta
     3361 tttgcaaaaa atacaaaaat ataaacgtta aaaaaataac gttatcctta agtttttaga
     3421 tgattttatt agtgatttgc gtaaaaaata aagtgatatc tagt
//
LOCUS       DAEPOG010000263         3337 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEPOG010000263 DAEPOG010000000
VERSION     DAEPOG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.265"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(154..1368)
                     /locus_tag="KR432_003537"
     CDS             complement(154..1368)
                     /locus_tag="KR432_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0642041.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            1783..2499
                     /locus_tag="KR432_003538"
     CDS             1783..2499
                     /locus_tag="KR432_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBH0642042.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            2634..3260
                     /locus_tag="KR432_003539"
     CDS             2634..3260
                     /locus_tag="KR432_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0642043.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
BASE COUNT         1265 a          419 c          516 g         1137 t
ORIGIN      
        1 tagtattaaa aaagattaaa aaattaaaaa actaaacagt taaataatta aacgattaag
       61 tgactgtcat ataacaattt ataattttat ttaacagcca cttaggtatt taagagcgat
      121 agaattgagt gtatatcaaa ttataataat ttcctagtaa ataattgaaa aataaggtag
      181 tccattttta tatttgatat ctggtttacc ttgaataagt ggatatagat aatttagggc
      241 ttcatcagtt atataattac attcttcatt tatccactct ttaggaaaat attttgtctt
      301 gttagctatt ttagaaatat cagtatgact aacttcatac ttataaggat tattgcatgt
      361 tctattaatg atagccataa ctcctgtata tcctttttta gatagttcta tcccatatgc
      421 accaacttca gctgcttgtt ttaaatcagt atctgaagct aaatgcatag cacatctttg
      481 tattacacca agttctaagg tttttactct tgttgtaatt gatgatgata ctataagatt
      541 ctttagatag ttgcttactc cacctaattg ggaatgacca aatttatcat gagcagaagt
      601 tttttgttcg catataaagt tcccgtattt atcccttata ccttctgatg caactatata
      661 agccttattt ttttctttaa atatttttct aacatcttct ataaacttat cttcatagaa
      721 atcaatttct ggaaggtata ttaaatcaac aagtggagta tcattaagtc tagctagtga
      781 agaagaagct gctaaccatc cagtatctct acccatggtt tctactataa atataccatt
      841 atttttgtat atagaaaaat ctaagtatgt ttccattatt agtgtagaaa taagctttgc
      901 agcagaacca aatccaggac agttgtcagt tcctaataaa tcattatcta ttgttttagg
      961 aatccctata aattttattt tacaagtagt gtttttgcag tattgagaaa atttcatcac
     1021 tgtgtccata gaatcatttc cgcctatata aaataatgta tctatttcat attcatctag
     1081 tatatttata actctatcat aatcataaga atttatttta taatcagcca gtttgtatct
     1141 acaagaacca agaccagacg aaggtgtttg ctttaaattt aatacattat ctttgtttaa
     1201 ggagtctgaa tctattatat ttttgtttaa tataccttca atcccattga taccaaaata
     1261 tactttatca aagtatttag tttctaaatt tttttcataa ataccaactg cagaagcatt
     1321 gataacagct gtagggcctc cagattgggc aataatacag tttctcattt tacatctccc
     1381 tttcattaat attgttatga atggtacttt ttatcgagta aatttatatt tatagtctga
     1441 ttatatatta aatgaactag ctatatcaat tgagttagta tagacaataa atgccataag
     1501 aatgtggtaa aaaactaact ttttatatac aattaatgtc tataatttac cacaatataa
     1561 aggtactttt aaggagtagt caaagtgttg tgaaaaaaaa tatatcagtg taatattaat
     1621 ttacaaagca aaataagttc acagttaaca aaagcagaat aagttcataa aagaaatata
     1681 tacagcaata aaagtaatgc taaataaaag taatactgga ttaaaaaata tcaaaaacta
     1741 atatttaata ctaattaaag taaaatatgg aggtatacaa atatgaaaca cataaaaata
     1801 gcagcaggat tagcacatgt aaattacggt catatatcag ccgtagttaa agaagcaatg
     1861 gatgcaggtg tagactacgt acattcagat gcagcagata tgcatgactt aaaaaatatg
     1921 caacttatgg gtggtcatca aataatagaa gcaataagac cagtgacaga taaaccaata
     1981 gaatgccata tatacacaag agattgtgat agattattta tagaaaaaat agcagcagca
     2041 ggatgtaact tgttgataat accagcagaa cattttttag gcgctcctct tgcatacata
     2101 ataaattact gtagagaatt tggtatgaaa gtaggattaa caattggatg ttacacacca
     2161 ctatgttttg ttgatgaagc tatatatgat atagatagat tgcaaatagt cgttcatggt
     2221 gttgatgaaa ctgatggaaa agataactgg ggatggagaa agagtgctgt tgatttagta
     2281 aaaagagcta gaaaaatgat agacgaaaag aatcctaagt gtgaattagc aatagatggt
     2341 ggactaagag cagacaatat ggagccatta atagaatgta atccagacgt tgtaatatta
     2401 tcttcagcaa tattcaagga taaagatggt ataacagcag gtgttaagaa ttgtagaaaa
     2461 gctatagatg aagcagctac aaagtttggt ttagaataag ataataaact atgataaaag
     2521 agatgctttc ttaatgtttt aaggaagcat taattttatg aaaaactaca ggaagctaga
     2581 ctaatttaat tgatttttta tactgaaaca atataatcat aggaggttat taaatgaaaa
     2641 aagaaatgat aaaagattat ttactgataa caatatcagc atttttaatg gcattagcag
     2701 ttaatttttt ctttgcagaa catacattag ctccaggagg gataacagga ttatcagtcg
     2761 tacttagcac attcttatca atgcctgttg agaatatatc attaggaata tctatgccac
     2821 ttttagtaat gggattatta tttttaggga aaggttttgg aataaaaaca ttatatataa
     2881 ctcttatggg tccattattc ttaaaagtaa taccacaaac tcacataact gacaatctat
     2941 tagtagcagg agtgataggt gggttattgg taggaactgc tatagggata gcaataataa
     3001 ggggctgtag tactggtgga actgatttag ttgcaatgct tattaataag gtaattaagt
     3061 ttctgaagtt gccagttata ttatttatgc tagatggatt tataatagta gcatctggaa
     3121 taataagtaa gaattttatg atatctatat attcattgat atccttatta ctcataataa
     3181 aaacaatagg atttgtaact acaaagtttg gtaagaatac tgaagaaatt aataatataa
     3241 ataatgcttg cgctcaataa aaataataaa atttacttta aatataaaag tttaaaaaat
     3301 ataaaagttt aaaaaatata aaagcttaaa taaaaag
//
LOCUS       DAEPOG010000264         3323 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEPOG010000264 DAEPOG010000000
VERSION     DAEPOG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.266"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            200..1021
                     /locus_tag="KR432_003540"
     CDS             200..1021
                     /locus_tag="KR432_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0642044.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1100..1311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1398..2714
                     /gene="argH"
                     /locus_tag="KR432_003541"
     CDS             1398..2714
                     /gene="argH"
                     /locus_tag="KR432_003541"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH0642045.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2963..3301
                     /locus_tag="KR432_003542"
     CDS             2963..3301
                     /locus_tag="KR432_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBH0642046.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
BASE COUNT         1388 a          298 c          628 g         1009 t
ORIGIN      
        1 aagactataa attgaaaata ttcagaaaaa tactacaaga aacgatttat tttgctctta
       61 aaataagtta aatgaatgtt attaattttg taaattgaaa tagtttaagt ataaagttat
      121 gatataatct aataagggta attctttaga ttatattaag gaaatatagg tatacaataa
      181 aaagttatgg agttggatta tgggatataa attaattgta acagatatgg atggaacttt
      241 attaggcaat aatcataaag ttacagatga aaataaaact gcactacaaa aagttataaa
      301 aagcggtatt aatgtaactt tagcaacagg aagagccttc gattcagcta aatgcaatgt
      361 agattttctt aaagaggaca tgccaataat agcttgtaat ggttcattaa taagagagca
      421 aaatggaaat ataatatatt caaataagat tgatacaaga acatgtttaa acattttaga
      481 tgtattagat aaatacgata tttactacca atgtaatagt atagattcta tgctttcgaa
      541 aaaaatagaa ggacgtgaag atagattaag tgtattttta ggctctgaaa cagaagtaat
      601 tgtaaaagat gatttgagag aagaaatctt taaaaaagat atactaaaat ttgtaatcat
      661 agaagaaaaa aatccatcta ttttggatga aataagaaaa gaattgagaa aagttcaagg
      721 aataaagata acttcttcat ggccaaacaa tattgaagtc atgaatgaag gtgttgataa
      781 aggtaatgca gttaaaattt tagctgaaaa aatgaatata gacagagaag atataattgc
      841 atttggagac aattacaatg acatagaaat gataaagttt gctggtcttg gggtagctat
      901 gggaaatgca gaagaattga ttaaacaaga agctgattat gttacagata caaatcaaga
      961 cagtggagtt gcaaaggcaa tttataaatt cctagagtta aaagaatcac ttagctctta
     1021 aaacagtata taataggaaa aaataggttg actattttgt agatagtaat tataatacaa
     1081 atagataaaa cataaaaatt tagattcagt gaaaaagaga gtagatatag agaaaatctc
     1141 agcgaattgg gggatggtgt gagcctaatg ataacacttt atattgaaaa gaactttgga
     1201 gaatactact tgaaattcag tatggtagta ggtaatctcg ccttaagaga atagagtggg
     1261 taaacattgt ttaccaacta gagtggtaac acggaatatt tcgtctcttg gtctttttga
     1321 ccaagagatt ttttagtttc attgagttac aagacttata attaaaatac aaaaaattaa
     1381 aatatgaggt gaaagttatg aaattatggg gcggacgctt tagaaaagcg gaaaatcaac
     1441 ttatggaaga atttaataaa tcttttggct atgattgtgt actgtacaaa aaagatatcg
     1501 aaggaagtgt agcacatgtt catatgcaag taaaatgtgg acttttaaca gaagaagaag
     1561 gaaagtctat aacagaaggt ttaaaaggga tacttgaaga tgtagaaaat ggaaagttag
     1621 tattggatgg agagtatgaa gatatacata gttttactga gataaattta attcaaagaa
     1681 ttggagatgt tggcaagaag cttcatacag cacgtagtag aaatgaccaa gttgcagttg
     1741 atatgagatt gtatgctaaa gaaaaagcaa atgatttggt gggtttaata tctgaattta
     1801 aagctactat aaaagatgtt gctgacaaaa acccagtgat gatgccaggc tatactcatc
     1861 ttcaaagagc tcaagtagtg acatttaagc atcacttaat ggcatattat agtatgtttg
     1921 atagagatga gaaaagaata aaaaatgcta tagaaatatt agatgaatca cctttaggct
     1981 gtggagcttt agcaggaact acgcatgata tagatagaag tataacttgt gaacaattag
     2041 gatttaaaaa agtagtagat aattttatgg atggagtaag tgatagagat tatttactag
     2101 agctaatgag tgatttttca attataatga tgcacttaag tagattaagt gaagagttga
     2161 tactttggag ttctcaagaa tttggatttg ttgagataga tgatttatat acaactggaa
     2221 gtagtataat gcctcagaag aaaaatccag atggtgcaga gcttataaga ggtaagactg
     2281 gcagagtata tggaaactta tttggtcttt ttacagtaat gaaaggaata ccattagcat
     2341 ataataaaga catgcaagaa gataaagaag gttttttcga tagtgtacat actcttgaaa
     2401 tgtgtataca aataatggat agaatgatag ccacactaaa ggtaaatgag gataaaatga
     2461 aacaagcagt aaaaaatggt tttttaaatg caacagaagt tgcggattat ttggttaaaa
     2521 ataatgttgc ttttagagat gcacatggta tagtaggaag tatcgttata tattgtgaag
     2581 ataataaaaa agcaatagaa gatttaacat tagaagaatt acataagttt tcagatgctt
     2641 ttaaggaaga tatttatgat tttatcgact atgagagtat attaaataag ggaataaaaa
     2701 agaatttaaa ataatgtatt tatttttaaa agctgtaaat ttgaatgtta tcatttaaat
     2761 ttacagcttt ttattgtgta agattttatt atataaaagt aagaaactta aaaggagtag
     2821 tatttaaaat aaatacaaat aaaaaattat tattaaaatg ataattaata tcaataagta
     2881 ttgacaaaat tatttctaaa tactattata tataatataa ataataatga aattcaatat
     2941 caaataaatg ggaggaaatc taatgaactt attagtgatt ggtggtcatg aaagaatgga
     3001 aaaagattat atggttatgg ccaagaaaaa aggttataag acaaaggtat atactaccat
     3061 gtcatctaaa ttaaataatt ctataggaag accagatgca gttgtcatac ttacatcaac
     3121 tgtttcacat aagatgtcta gaacggttga gtctcaagct aaaaaacaag atatattaat
     3181 cgtaaggcat aaaaatagta gtaaggtagc atttaatgaa tgtctagata tggttgatga
     3241 atgtctggga aattgttcaa aatgtaagct taaagacaaa caaaaacaaa aaaatatata
     3301 ggtataaaaa atacataaac ata
//
LOCUS       DAEPOG010000265         3323 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEPOG010000265 DAEPOG010000000
VERSION     DAEPOG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.267"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..718)
                     /locus_tag="KR432_003543"
     CDS             complement(<1..718)
                     /locus_tag="KR432_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0642047.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQI"
     gene            complement(758..1105)
                     /locus_tag="KR432_003544"
     CDS             complement(758..1105)
                     /locus_tag="KR432_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642048.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1323..2279)
                     /locus_tag="KR432_003545"
     CDS             complement(1323..2279)
                     /locus_tag="KR432_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH0642049.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2720..3088
                     /locus_tag="KR432_003546"
     CDS             2720..3088
                     /locus_tag="KR432_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBH0642050.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1206 a          432 c          421 g         1264 t
ORIGIN      
        1 atatttgttt aagatatatt attatatctt ctggagttct atcaaatttg agttcaaaat
       61 tttcatcttc aaagtaggga atataatata aaggtgtttt gagagcaggt aatttattat
      121 ataattcttc aagaatatcg atatttttag gggagttaaa gaaaaagcat ttacatacgt
      181 tcattaaatc cacagcatct gaaagtgagt tatgtttaat atctccttct acagaaaaaa
      241 tttttttcat attcagtaaa gatatttgct tagataagat tctattattg tatgtaatat
      301 gttgagatat aaaaggttga atgtcaatca tcatattaaa taactctaag catttacctg
      361 tatatttata tactctaaat gaagttagaa ggccagttat atcaaaatca ccccaattat
      421 atattgtaac atcatcaaat ttacaaaacc atttataaaa atcatccata acatataaaa
      481 gattaggagc attattgata tctagttggt tcaatttggt taaattttta catttatttg
      541 aaagtatttt attgtatttt ggcttaacta aagagtaata attatctatt gtattaaatt
      601 cgttatcaac taaaactcca ccaatagaaa ttaattccgc accattatat tcttttggtt
      661 tatttttatt atcatctagt atattaaatt caaagtctat aaataacttg tatttcataa
      721 atctaaagca catcctcagg taaattattt attgagccta agcatcataa gaatcaatag
      781 atataatgac tctaaacatc aaaggttcat tacaactttt atttatttca taatttaatt
      841 caaataaatc ttttaagata taatttataa gtattctttc ccaacccatt tttaaattaa
      901 ctttagagtc tattaatata ttttctatct ttttaaatat ttctatattt gctggtatta
      961 tactgagctc aatattactt ttaaacgggt tagactgtaa atctgttata agtatatttt
     1021 ttaattcctc tgtaagttca gaaattaaac tatcacctat gtattttttt atgatttcct
     1081 ctctaatttc ttttaaattt agcaatatta gtacctgcta cttacatagt agcttttcct
     1141 ttccttttaa tactattatt catttttgaa tcttatttag atatatttta agattaaaag
     1201 tctttagcta tataattttt tacccatttt taacttatat taacatatta caggcttata
     1261 tctccaccct gcttacattg aggatggagt atattgccta ttttgataaa aatgataggt
     1321 tgttatttgc ctattccttt ttctgataat ttttctaaca tatctttggt cattttatct
     1381 aaatcataac taaacttaaa gttccattct tttactgcac tactactgtc tagagagtta
     1441 ggccaagaat cagctatact ttgtcttaca gggtctacat catattctat cacaaaatca
     1501 ggaatatatt tttttataga atcagctatt tcttcaggag aaaagctcat agcagtaata
     1561 ttaaaagcat ttctatggat gagtttatcc gctggagctt ggattaaatc cactattgat
     1621 tgaagtgcat caggcatata catcatatcc atttttgtac cttctgctat gtaagattta
     1681 tatctcttat tttttaatgc ctcataatat atatcaactg cataatctgt agttcctcca
     1741 ccaggaggcg taacataaga aataagacct ggaaatctaa cacctctagt gtctactcca
     1801 aattttgagt gataataatc acatagtaat tctccagaaa ctttagtcac accatatata
     1861 gtattaggtc tttgtaatgt atcttgagga gtcatatttt ttggagaatt ttcaccaaaa
     1921 gcagcaattg aactaggtgt aaaaaattta caatccaatt ctttggctat ttcaaggcca
     1981 tttattaatc cattcatatt taagttccaa gcttctaggg gtttactttc agcaactgca
     2041 gaaagtaaag atgctagatg aattatagta tctacatcat atttttttgc aatttcaaaa
     2101 aaacgattct tatctgtaac atctaatatc tcaaatatac cagattcaca tactggatta
     2161 ccttctttta ctctccttga actagcgatt acattttgtt ctccatactc atttctaagc
     2221 tttattgtta attctgaacc tatttgtcct aatgcacctg ttataagtat ttttttcaat
     2281 tttacaacct cctaagggtt taatttatgt agtactactt atttttattt tagcaaatct
     2341 tttgtttaaa tacaaagaat taaagataat aatatacgac ttaattgaat gaagtgcagt
     2401 aaattcctaa aaatttaaag ttgagatata atggtctatt gtataacaat aaaagactag
     2461 attaaattat tatatagatt gtgtgactaa gtcacagaaa gtatagttaa tttaatatat
     2521 aataaaagca tatcaagtaa agctttaaat taaagtaact ttatataaaa agtaatataa
     2581 attgataaaa ttataaattt atttatagtg aatataggtt ggattttata gatacaaaaa
     2641 tagtatatat gatatgtaga ccattagcat gtttaaagta gcaaaacata taaaataaaa
     2701 tttgaaagga agtataaaaa tgacaagacc atcaatatat catagccaag gatacacttg
     2761 tgcagaggca ttaataaagt cttataatga ggaacacaac acagatatac caatctcaat
     2821 aggaagtggt atgggggttg gaatgaatgt tggaagtgtt tgtggagctg taaatgctgc
     2881 tgttgttata atagggtatt taaatgggcg aaatagtaat ttagatgaaa atatagcaag
     2941 gaagtattca agagagctat tgaataaagt aagaaataaa tacagctcag aaatatgtgt
     3001 atctcttaaa aaaaataaag taacttgtga agaaataata aatttcacat atgatgctct
     3061 aaatgatatg ctaaaaaatc aaaaataatt ctaagaaaca agtattttac attcgataga
     3121 atacttgttt ttttatgata tttttaatag gtattagttt attataattt ttaaatcaat
     3181 tttattatat tttaattgaa ataatctact ttgaaaatta ctaactgaca aaagaatatg
     3241 attatagtat agaaaaaatg taaattattt aatattaagt gagagtatat gcttacctat
     3301 ataaaatcgt ataaattaat ggc
//
LOCUS       DAEPOG010000266         3313 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEPOG010000266 DAEPOG010000000
VERSION     DAEPOG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.268"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..712)
                     /locus_tag="KR432_003547"
     CDS             complement(<1..712)
                     /locus_tag="KR432_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0642051.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSK"
     gene            complement(743..2977)
                     /locus_tag="KR432_003548"
     CDS             complement(743..2977)
                     /locus_tag="KR432_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0642052.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1154 a          446 c          382 g         1331 t
ORIGIN      
        1 atttagagaa tacttttaaa aaattttctc ttaaggcttc tagttgtgaa taaacataat
       61 ttttgtcaac atcactagag aatataacaa taaattcatc tcctcctaat cttataatag
      121 aagtagaatt tattaattct ttatttaaaa gtgaaacaaa atcacataaa atcttgtctc
      181 ctacattatg accttcatag tcatttatca ttttgaaatt atctaagtca attagtacaa
      241 tataatctcc tataaatatg tcaaatctat catttaaata ttttctatta tatgctccag
      301 ttaatgggtc agtaattgag tagtgaagat ttctcttatt ttcttcatag agttttgtta
      361 tatcattggc catagtaatt atgtaggttt tttcatcaac atctactttg tattctaaaa
      421 cattccaata gcttgtagta tcttttttaa actctaaaat ccaatttgaa attttatcat
      481 aaggataatt tttttcccaa tctaattgaa tattgtgaga atccgtaaaa aatttagcaa
      541 tattatcagg gagtttctta gctatttcta tattagtcat tccaagtgga ttatcttgca
      601 tataactttt aattaaattt tttaaattat catttataaa tataattctg tagttagaat
      661 ctcttataat tagaaatcct ggaaaagttc ttaaaaaaat ttctttaaac atattaattt
      721 ctccaaaata aaatactttg tactagattt ttgttaaatt ttttatttta tttacatcta
      781 catacatacc ttgggcaatc gaccttccat caattgcaga aaggttacat tgtgtataca
      841 taactgtgac aatttgacca tctcgtttta tgaatctata taaaaatggt tcaattttat
      901 ttgtgtcaat catatccaaa ctttttttat aaacaaagtc taaatcatct ggatgaacaa
      961 ttgctcttaa atttccgccc atctcaccaa aaaactgttc ttttgtataa cctataatgt
     1021 caaacatttc ttcattcata taagtaataa tcatatcacc aaataaatcg tagcgaatta
     1081 tacctacagg caacattgaa taaaattgtg ataaaatatt actttcatca agatgtgtat
     1141 ttgtcatttt aggtattgaa tatgattcaa attcatttat atagccagca gttttaatta
     1201 aatccaaatg taattttgct aagagtaatt tattttctga aattatacaa tttgcactaa
     1261 agtagaaatt gctatatttt tgatgttcag aagcttcatc tgataaaacg gcttctccac
     1321 taattaataa tgaatctgat tcagtattat gtggggttat acttttgtaa agaatagaaa
     1381 attttttacc tttaatatgt ttttcaaaat gttctattac tttaggaaaa gatgtaattt
     1441 cagttttgtt gtaaaaatca aaccattgaa tgtcttcagt gacattagct cttagttttt
     1501 tcatatcttg cataataaaa aatccatcaa atatcttctc tattaaagat tttcctatat
     1561 cagaatattt tataaacatt ttctctgaat tattctcttt agatagtaat ttttcaaatt
     1621 ctttgcactc catgggtttt gcataataat aaccttgggc gatatcacaa tttatacttt
     1681 taataaattc tacatgttgt tctgtttcaa caccttcaca tactgtaagc atcccaagag
     1741 ctcttgcaat gtttattata gagtacaaaa tggttgtatt tttaaaatca tttaaattat
     1801 gagataaaaa tatcctatca attttgatta tatcaaaagg tagattttgc aacataccaa
     1861 aagatgaata tccagaacca aaatcgtcca ttgatacaga aaaaccataa gaacgaagtt
     1921 cagctagttg ttgaataatt gtatttgtat tttctattaa tatattttct gttatttcaa
     1981 tgctaaaatt tgatgtagag acgctgtact tttcagtaat accttttagc atacttggaa
     2041 aattagggtt aaaaaaatga agacgagaaa agttacaaca aattggaaca aaaacatcat
     2101 tgttatcaat ccatcttctt atatttttac agttctcttc aaataaatgc aaatctaatt
     2161 ccgttatcag accaaactgt tcaaataaag gaataaattt atctggagta agaagtcctt
     2221 taatagggtg tctccatctt gcaagtgcct cacatccaac tatagtatta ttttttatat
     2281 tataaagtgg ttggtaaaat acttcaattt gtttagtttg tagaacatgt gatatttctg
     2341 ataagaactc cttatcattt tctaatttat tgtaaaaact attatcttta aaatcaaata
     2401 gaatgtatct atttttgcct tcacttttag ccttttttaa agcactatat acttggttga
     2461 aaagtatcaa agtagtcatg tttggttcac cattccagat agtgctgccg atactaaatg
     2521 taacattgga gtatatttct ttttcattag gaagtcttat ttttaattct cgggagctct
     2581 ctataatagt tgcacccata ttatttaact ctttatttga tacatttgga agtacaatta
     2641 aaaaaccttc tccttttaaa taagcgacta ttccctttga ttctggaata aagtgcaata
     2701 ggttttgagt tagagtaatt aataaatttt ctccgactag atatccatat atatcattaa
     2761 ataatttaaa gttatcgata tttaataaca tgattcctat atgtatattt ttcgaaatac
     2821 agtcatcaat atattggtac ataaattctc tgtttggtat tttagaaaaa aaatctattt
     2881 catttaaggt ttttaattta aaatttctac tatctaattc cttagataat acttctacct
     2941 ttttatttaa catttttata taattatgat tttgcatggt attcctccat aagatacttt
     3001 aaattttgat gacaactgta tttatgctag aagtatttaa atgttaattt gtaaataatt
     3061 taaatgctaa attttaaata agtatttaaa atgaatatac attaagttat ttgctccaat
     3121 gctacaaata caaatatttt ttagtttagt aataatatta gataaaatta gttaatgctt
     3181 aaattatagt atattgaaag atttcctttt tgaatgcaat tatatcatat tttttcataa
     3241 atagatagat tgtgagctgt atagttagta aggtagagtt tttttataat taattttaag
     3301 tttaatgatt ttt
//
LOCUS       DAEPOG010000267         3290 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEPOG010000267 DAEPOG010000000
VERSION     DAEPOG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.269"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..632)
                     /locus_tag="KR432_003549"
     CDS             complement(<1..632)
                     /locus_tag="KR432_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642053.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKI"
     gene            complement(625..2559)
                     /locus_tag="KR432_003550"
     CDS             complement(625..2559)
                     /locus_tag="KR432_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0642054.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            complement(2588..>3290)
                     /locus_tag="KR432_003551"
     CDS             complement(2588..>3290)
                     /locus_tag="KR432_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBH0642055.1"
                     /translation="AKIIGIGVEGINIINEVEEKIKANMDIEKININQEIEKEYVRSL
                     LDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLELGREFKI
                     NNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFEDTKSYSEI
                     ADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYEVIFSLYI
                     KENLNGNIRVGLLYN"
BASE COUNT         1061 a          579 c          241 g         1409 t
ORIGIN      
        1 aaaatttttt taatcttgcc ttgaattgaa ttgtcattta ttctacataa aatagatata
       61 catgcttttt taagaacttg attcaagaaa ttattttcac tacactcatc atattcacat
      121 cttattttca ttggagaaat gcctttttct tttgcatatg tacttaataa tattttaccc
      181 ctcataacat ttaagttctc tattttattt atatattcaa aataaatacc ttttttcatc
      241 tgtgtttgca tactctcaat gaaatacata acaaaaaaat ttaagagatt ataatttttc
      301 aaactcaatc ttattttttc attcattgta attggaattt tattacatat agaaagcatc
      361 tgtaatagta tctctctatc ttttacaaga ttatcactta atgacaattt aggaagtatt
      421 tcaagtatta cattttcaaa acatatgatg cctacatagt ttataaatct aatttttttg
      481 tatttaatat ctattatatt actttttaat acatcattat tgttctctag atatttaagc
      541 aatttttcat attcaatttc tgtaagctca ttttcaccat tacctaccct tatccattca
      601 taggtttcct taacatatat atttttactc atatatattt tttagctcct cactagatat
      661 gttttgtttt atacaatagt taactttact ctccattatt gaggcattat tatttttaaa
      721 taactcagaa gctataagtt tttctttata aacaatgtat ttatcttttt catttcttcc
      781 aattcctcct aaaatcaaac ctactttttc tgaatcataa taaaaatact cttgaagaag
      841 tggtattatt ttacttctaa atactactac tatatcatta atattttcta cagcaagtag
      901 ataagcttgt ccaataacat ggtctctatc atataaaaac tctatccttt tattgattgt
      961 atatagcatt tttctaatat ctataccatc tacaatagac aataaatcat aatctggcat
     1021 aatttcttca aaattaaacc ttcttcttaa agctatatct aaaagtgcta ttgatttatc
     1081 tgatgtgttc atagttccaa ctatatacaa atttggaggt agagaaaatt tttcctttga
     1141 atatggtagt ttgacaatag tttgatttga tttagtaagt cttttatcat cttccaataa
     1201 tgttattagc tcaccaaata ttttagatac attccctcta ttaatttcat ctattattaa
     1261 aacatagttt tctgattctt caaaattaaa actctcacta tattctatat tttctaatac
     1321 taatttcttt ttcctgtctt ttatagtttc taaagtcaag ctatcttttt ccaactcaaa
     1381 gctcatcttt ttttcaaatt tcaatgcatt ataacatgct tctatagata tatcctttaa
     1441 tatcccatct tctggcttaa aattgccttc accatcagac ttaagaccct ctataaattc
     1501 ctcatatcca tatgattgat gaaatgtaca aaattctaca ttgtttttat aatctaagta
     1561 acctgtatag tatttgtttc ttagactggt ttcacttact agattgtaaa ccatctgttt
     1621 tgtattgtat gttttaccag ttcctggtgg tccataaagt atagtattta atggcatttt
     1681 attagtaaat ctttttacat acttttcaac tatactatta ttttcaaata taacccatgc
     1741 atttccgtct atattctcct ttccatactc tatccatcta acaggaaaaa aatatccacc
     1801 atccaaagca cttactttct ctttatcgaa cccttccata acttgacata cgccaaatag
     1861 actcttcatt gtatggtcat tttcacaaat tactttctcg ctattataca ataaaatata
     1921 atcatctgtc tttatatttt ttaggctttc taattgttct agattaatgg gctgaataaa
     1981 taattcttca tcatctacac acttatctac atttttataa taatagtttt ttaaatacca
     2041 tttattattt tcactttctt ctattctttt tctcaataat ttcttatctt gtgacttaat
     2101 aatttctaaa tatgaactaa tatcattatt atttaattta taaaattcac ttgtatatac
     2161 tttacaattt gaaactgtat caaaattatg catccatttt actggtagtg tatgccctaa
     2221 aatatgactt aattcatatc cattttcaaa atcttcttca actatgccaa tagacacatt
     2281 acatttaact ttttcttcta tattttgata tgctttatat aaaaaataat ctcctttttt
     2341 aatcgacata aactttttta taaaatcttt atcattacaa ttctcattaa tatacttttc
     2401 tgattttttc cccatactga atactttttt taaatttatt tttatatcaa atccaagtgc
     2461 aattatgctt tcttctataa aaatctgtgg tgtgtctgtt tcctgataaa aaaattcttc
     2521 cattaaccaa tactctgtac ctcttatatt ttcagacata tacactctcc ttaatatcta
     2581 aaatatacta attatacaat aagcctactc taatatttcc atttaaattt tcctttatat
     2641 ataaagaaaa tattacttca tagctttctt ctaccttgct ctgaatatta cttattaaat
     2701 cattcaattc cattatagaa aattctggac ttccttcaac aaatacaatt cctttcttac
     2761 ttataaattc ctcaccaata tattccatcc tgtcaaataa aatgtcagct atctcgctat
     2821 atgactttgt atcttcaaac tcttcaaaag aatacttaat tcctttatct cctacaatag
     2881 cttcttttaa atcagtaata tctatattta tcatgcaaga atcagaaata gactctacca
     2941 tgatattcat taaatcaaca aatttaaata tactatcatt attgatttta aactctctcc
     3001 caagctctaa atcttcttta ttttctttct ctgaacaatc cattcctata gatagaactc
     3061 ttctttcgtt tgacatatat gaaacagctt ttataatatc tctaatatgt ttatcttcag
     3121 atgaatatat caaaaataat atatctacac catctaaaag acttctaaca tattcttttt
     3181 caatctcttg atttatgttt attttttcta tatccatatt tgcttttatt ttttcttcta
     3241 cttcatttat tatattaata ccttcaactc caataccaat tattttagca
//
LOCUS       DAEPOG010000268         3266 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEPOG010000268 DAEPOG010000000
VERSION     DAEPOG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.270"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(281..1420)
                     /locus_tag="KR432_003552"
     CDS             complement(281..1420)
                     /locus_tag="KR432_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0642056.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
     gene            1757..3010
                     /locus_tag="KR432_003553"
     CDS             1757..3010
                     /locus_tag="KR432_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0642057.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
BASE COUNT         1241 a          393 c          378 g         1254 t
ORIGIN      
        1 cctgttagtt atattaaaaa taatcttttt atataacttc acaatcaaac cttgtcttat
       61 ttcataatag ttttatttaa tatcatataa tttagacctc cttataagat tatttatata
      121 tataacgaat aataaaagta attttggaca aaataaaaat ctttttattt taagaatgaa
      181 aaatttaacc ctctaagaaa actcaattaa gagcgattca tagagggctt attattatat
      241 tatataactt tatatacttt ttatttttat atgcttcaat ttaacttatt tcaagcatat
      301 tcaagtactt ctcattaact ttatatacat ccttttctct agataaaatc tctatttctt
      361 caagattatt aaacacttcc atcaactggt caactaattc gatttctttt attccagcct
      421 ttaagtatct acctttattc tttttgatat agttctctaa tgtcatagaa taattatctt
      481 tactacagat atattttaat atatgttctt ctgttgctga tagagttata tcattttcaa
      541 ataattcctc ttttaatata ctctctaaag acttcctatc atgcatatca cgcatttcat
      601 tgatagattg tattatagac tttagcatga actctataaa tacagttaaa ttatatcctt
      661 tattctctac atctagtata gccttataat acttagttct gtttttagat attatatatg
      721 atattgaaaa ctctttaaaa gtatcatatc ctttattaat taaatataga tatgataagg
      781 ctcttgatgt tcttccatta ccatcactaa aaggatgaac atatacaaag tagaaatgaa
      841 ttattgtact ctttactaaa tcatttactt tactattttc cataaaaatt attaattcat
      901 ccataaaatt atatattttc aaaggatcaa gtcctgtatg tataatctca ccttttgaat
      961 cacatacata tactttttca gttctatatt catattcttc tgaatctaaa cagttttcac
     1021 ccaatatatg atgtaaatta tatataaatt tgtgactata taatttatct aaattatcta
     1081 atccatattc taaggctcta tgattattgt aaatcatata ctcactttta ttacttggtt
     1141 tcatctttcc tctaattata gattttgcaa tctttctagt tgaaaaggca ccttctatta
     1201 cactagaata ataagactct aaggctaaat catttttatc ttctattatt ttaaatatct
     1261 tgcttgatgt taatctatct atttcatctt ttgaatttaa tatgttatca gtttcatgat
     1321 agtatagttc tttattatca aaactcttta aggaaagtat aattttatct ccagtagagt
     1381 atttattttt gtattcttct attgtcagtt taatcaccac cttagtttat ctttatctat
     1441 taatattaac aaaaagtatt agtttttaca ctatttttga ttgtttattt tatatattat
     1501 gattctactc ttgataatta aatcttactt aaaaattaat tcttgataat taataaaaaa
     1561 atttatatat aagtgtctaa aaaacgattc tttattcgtt atatatataa ataatactag
     1621 aaagggagga ttatattatg atatttagta aacgctcttt tagaaaaaaa cggtgataaa
     1681 cttgttttaa attttagtag aatatgattt ttgttttggc aagtattaat acatatatta
     1741 aagagggagg tttaatatgt taaataaaaa aaatataagt tcaattatta tgacaatgat
     1801 tttaagctta tcaactactg tatttgtttt cgctgatgaa aatactaatg tgaaaggaca
     1861 agatgtaacc aaatataatg aagaaatggt ctctaaaaac ttagaagatt taagtataga
     1921 acaattaata gatattagag ataaagctat tgataaaaat ttagaaggtc ttagaaaatt
     1981 tggagaagat tcagtgaaag aacgtaaaga ttcaatttta aactttgaat ttggattagt
     2041 agcttttgtt aacaaaaatt atagtaactt aacagaaaaa gagaaattaa taagacaaat
     2101 caatattgtt gagtatgaaa attctataaa tgatataatg gatccttcag gcgatttttt
     2161 caaagtaaca aaccaaagtt atcagattaa tgaatttgta ttaaaaaatg gaaaaaacta
     2221 taaagagtgg tcgtatgaat atgtagagtt tttgattgag catttagagg atttatgtaa
     2281 ttttgtagat tttgatgcaa aatatgtaat agaaatggtt gatccttcaa taaaaccaga
     2341 tctttcagat aatcaaaaga aatctctaaa taatgaatta ataaaaatgt attcaagtaa
     2401 tactctaagt acaaatataa aagatgaaat agaaaactgg gaaaataatt taaatattaa
     2461 tgagaaaaat tcaaatgaac ctttaccaag agtaagccct tctagttata gacaacaagc
     2521 attagcttat gctaaaaaac atggctatac taatggttat tatggtagta caaatagaaa
     2581 aaataatgca ccttctccat attataattt tcaaaaagat ggatatggag attgtgctaa
     2641 ctttgtttca caatgcttac atgaagctgg agtaacattc tggacgaata ataatccatg
     2701 gtattattat tctactagta atagatcacc ttcttgggct ggagcaagag aatttaaaat
     2761 acattggatg aatcgaattg catatcaaac acttactgta tcaaactctt taagtttttt
     2821 aagaccagca acacctgtaa gtatattaaa tagtagtgga gtagcaactc atacactaat
     2881 atctacagat aagcattcaa atggatataa tttttcatat gctgcacatt cagatgaagg
     2941 tgcaagaaca aatttgttaa aaaaacttga aggaaagaaa atttcttatt acaaagttta
     3001 ttgggaataa ataaaaattt atacttttaa ttaaatgaaa attgaaaaga ataaatatct
     3061 atttcaagat ccaataagta tttagttgaa gcagctatta aattttatta cataatcata
     3121 aaaaagagac tagtggaatt tagtctcttt tttatgattt aaaaatttaa taaaagaatt
     3181 atataaattt gtctaaaaat tagtttatca ttcgttataa ttatataaac tatatcaaaa
     3241 aagtgttcta ttgtattctt tagaat
//
LOCUS       DAEPOG010000269         3197 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEPOG010000269 DAEPOG010000000
VERSION     DAEPOG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.271"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1303
                     /locus_tag="KR432_003554"
     CDS             <1..1303
                     /locus_tag="KR432_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0642058.1"
                     /translation="ENFLKHVQNYIISNEKENCVMACFDLCRLKLINDIYGYELGDEV
                     LNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLENREQKIPYLVNLIKNKIMILSQIDS
                     FKMDINIEASIGIYKFNNNEIDIKKAIDNADMARLKSKGLKHIEYVEFDNAMEEEIQS
                     IMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIGSEALIRWLHPSLGMVGPNKFIPIA
                     EKNGLINNIGRWLIQEVFITINKWISEGINVLPVSINLSRVELYKNDLVDFFKLMFNT
                     YNIPKELIELEITETTALRDVKFISERLYEIKSLGMNISLDDFGVGNSNFINLKGIPL
                     DIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLNVTVICEGVEDMHQVKVLSELGCNF
                     IQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1306..1704
                     /locus_tag="KR432_003555"
     CDS             1306..1704
                     /locus_tag="KR432_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0642059.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1744..>3197
                     /locus_tag="KR432_003556"
     CDS             1744..>3197
                     /locus_tag="KR432_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBH0642060.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEY"
BASE COUNT         1342 a          276 c          545 g         1034 t
ORIGIN      
        1 tgagaacttc ctaaaacatg ttcaaaatta tattatcagt aatgaaaaag aaaactgtgt
       61 aatggcttgt tttgatttat gtagactcaa acttataaat gatatatatg gttatgagct
      121 aggggatgaa gtgttaaata atattttaag taacttaaaa aattattttg gaaaagaagc
      181 tatttatgga aagttaaaaa gtgatgtttt tgtattaata ataaagcttg aaaataggga
      241 acagaagatt ccttatttag taaatcttat caagaataaa attatgatat taagtcagat
      301 tgacagtttt aaaatggata taaatattga ggcttctatt ggaatttata aatttaacaa
      361 taatgaaatt gatataaaaa aagctataga taatgctgat atggcaaggc ttaaatcaaa
      421 aggactaaag cacatagagt atgtagaatt tgataatgct atggaagaag aaatacaatc
      481 aattatgaaa atagagagag atttattctt ggctattaaa aataaagagt ttgtaattca
      541 ctatcaacct aaagttgatt caagtacagg taacattata ggttcagagg cattgataag
      601 atggttgcac ccatcattag gcatggttgg accaaataag tttataccta tagctgaaaa
      661 aaatggtctg ataaacaata ttggtagatg gctaattcaa gaagttttta ttaccattaa
      721 caagtggatt tcagaaggta taaatgtctt accagtctca ataaatcttt ctagagtaga
      781 actatataaa aatgatttag ttgatttttt taaactgatg tttaatacat ataatattcc
      841 aaaagaactt atagaattag aaattacaga aacgacagcg ctaagagatg taaaatttat
      901 aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat atatcattag atgattttgg
      961 agtaggtaat tcaaatttta tcaatcttaa ggggatacca ctagatatta taaaaattga
     1021 taggtcactt atattggata tagttacaaa tacaaaaaca aagtttatgg tgaaagcaat
     1081 tattaacctt tcacatgatt taaacgtaac tgttatatgt gaaggggtag aggatatgca
     1141 tcaggtcaaa gtcttaagtg aactgggatg taatttcata caaggttatg tattttttaa
     1201 acctttggat gagatgaatt ataaaaaact tcttactgac ggaagttata taaatctgaa
     1261 agatactctt ttagataaat gcatgacagt tgaggaggag taaggatgat ttcagaagat
     1321 gtagattatt caataagaat cataagagct atatttaata atggagaaga taaaccaata
     1381 gatgctaaaa tgatattaaa aagtgaagat ataccaatac taacactata cagactgctt
     1441 agaaagcttt gtgcaaacaa tattcttact tttaacaaag aagatggcaa gacatacatg
     1501 ttaaatgtgt cattggatac aataagtcta agaactattg tagaagtaat tgatggaaaa
     1561 atatatataa ataaatgtac gagatatgta tctaattgca gtaattgctt aaaaggttgt
     1621 aaagtaaatc gtgaattgct agaaatagaa aaagaaattt ataaactttt atcgagaaaa
     1681 tctataagac aaatactaga ataaaaaatt ttcagctatg ataataattg gaggttttcg
     1741 gttatggatg atatatctca agataatttt ctactgtcta aagagtatga gaatagctta
     1801 gatgtagata ctaaaaaagc aagtggaata tattatactc ctaaaataat tgtagattat
     1861 attgtaaaaa aaactttaaa aaatcacgat ataattaaaa atccatatcc acgaatactt
     1921 gatatttcct gtgggtgtgg aaattttctt ttagaagtat atgatatttt atatgactta
     1981 tttgaggaaa atatatatga attgaaaaag aagtatgatg aaaattattg gactgttgat
     2041 aacatacacc gtcatatatt aaactattgt atatatggtg ctgatattga tgaaaaagct
     2101 ataagtatat taaaagatag cttaacaaat aaaaaggtag taaatgattt agatgaatct
     2161 gatataaaga ttaatctatt ttgttgtgat agtcttaaaa agaaatggcg atataagttt
     2221 gattacatag tgggtaatcc accctatatt ggtcataaaa aacttgaaaa gaaatataaa
     2281 aaatttctcc tagaaaaata tagtgaagtt tataaggata aagctgactt atatttttgt
     2341 ttttataaaa aaattataga tattttaaag caaggagggg ttggcagtgt tataactcca
     2401 agatatttct tagaaagtct atcaggtaaa gatttaagag aatatattaa atcaaatgtc
     2461 aatgttcaag aaatagtgga ttttttaggt gcaaatatat ttaaaaacat aggggtatct
     2521 agttgtattc taacttttga taagaaaaaa acaaaagaga catatataga tgtatttaaa
     2581 atcaaaaatg aggatatatg tataaataaa tttgaaacat tggaagaact tctaaagagt
     2641 agcaaatttg aacattttaa tataaatcag agattgctat ctgacgaatg gatacttgta
     2701 aataaagatg atgaaacttt ttataataag atacaagaaa aatgtaaata tagcttggaa
     2761 gatatagcaa taagttttca aggaataatt acagggtgtg ataaagcgtt tattttaagt
     2821 aaagatgatg taaagttgaa ccttgtagat gataaatttt taaaatgttg gataaaaagt
     2881 aagaatataa ataaatatat agtagataaa agtgaatata ggctaatata ctcaaatgat
     2941 atagataatg agaatacaaa taaaaagata ctagatgaaa ttatagggct atataaaaca
     3001 aaattagaaa acagaagaga atgtaaaagt ggaataagga aatggtatga acttcaatgg
     3061 ggtagagaaa agcttttctt tgaaagaaaa aagataatgt atccttataa atcaaatgaa
     3121 aatagatttg ctatagatta tgataacaat ttttcaagtg cagatgtata ttcttttttt
     3181 ataaaagaag agtattt
//
LOCUS       DAEPOG010000270         3130 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEPOG010000270 DAEPOG010000000
VERSION     DAEPOG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.272"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            12..737
                     /locus_tag="KR432_003557"
     CDS             12..737
                     /locus_tag="KR432_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0642061.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            741..2144
                     /locus_tag="KR432_003558"
     CDS             741..2144
                     /locus_tag="KR432_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0642062.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            2155..2979
                     /locus_tag="KR432_003559"
     CDS             2155..2979
                     /locus_tag="KR432_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBH0642063.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
BASE COUNT         1273 a          272 c          529 g         1056 t
ORIGIN      
        1 ggaggttgtt tatggaaaat cgtgttttaa taatagatga tgaagtagaa atattgaagc
       61 ttttagaaac tgtcctaaaa aaagagggcc ttaataatat atatactgca aaaactaaaa
      121 aagaaggatt agaattattt aaaagtatta atcctgattt gattgtattg gatattatgt
      181 taccagatgg cgaaggttat gatatttgta aagaaataag aaaaacctcc aattctccaa
      241 taatattttt atcagctaaa accgaagaat tagataagtt acttggacta gcgattggag
      301 gagatgatta tgttacaaaa ccttttagcc ctaaggaagt ggcttttaga gttaaagcgc
      361 atttgagaag attaagttat tttagtgatg ttcaaaacga aagtaaaaat ctaaataaga
      421 atgaagaaaa aattatatca tttggcccat atatattaaa tgaaagtagg gcagaactta
      481 taaaagatgg aaaatctata ggactgtctg caaaggaact aaaaatccta tctttatttg
      541 cacataatca aaatcaaatt ataagtaaag aaaaactatg ggataaagtg tggggagaag
      601 attatgttgg atttgacaat actataatgg ttcatataag aaaaattagg gaaaaattag
      661 aggataatcc ttcaaagcct gaatatattc tgacaataaa aggtcttgga tataaattag
      721 cagtaaagga agattagaat atgaagtgga agattactag aaattttata tttacaattg
      781 tgtttgtagc tatatcagta gtaattataa atattatatc tatattgtat gtgatttcaa
      841 ctaatagctt ttttaaagtt gtagattctg gaaataatcc tgaagaattt gcacgttctt
      901 ttgaaaaaga tttatatgaa aaagatggag aatttaaact atctaagatt ggagctgaaa
      961 aattagaaaa atcaaattcc tggatacagg ttttaaatga tttaggagag gaagtttatg
     1021 gagtaaatgt gccaaaggat actccaaaaa aatatacacc ctttcaaatg gtgaataatt
     1081 acaagtatat agaaacaaag tatgttaatt ttgtactaga aaaatattta aacaaaaaac
     1141 atctaaacat aatagtaggt atacctagta gagatatatc aagaataatt ttaacctatt
     1201 ctcaaaataa cattaaaaaa actttaaata aagttatcat tataacacta gtaattgata
     1261 gcgtggtagc tttaggtgta ggatatttat ttagtagaaa acttacaaaa ccaatttcaa
     1321 gcgtactatg gagtattgag actatggcta atggaaatta tagtttatat cttaaggaca
     1381 gaggtatata tgaagaagtt tttaaaaata taaatatgtt agctgatact ttaagagtaa
     1441 atgaagttga gagaaaagaa aatgaagagt tgagagagga atggctggca aatataacac
     1501 atgatataaa gacacctcta gcatctatac aagggtatgc agagataatt aatgacaaag
     1561 attatgagtt tgaagaagat gaaatacgag agtatacaga aattatatat aataagtcta
     1621 agtatataaa agatttagtt gatgatttaa atttatctac tcgattaaaa aataatacta
     1681 tagttctgga caagaaaaaa ataaatttag tcagtttagt cagagatata ataattgata
     1741 ttttaaatga taatagatat aaaaatagga atattgaatt tgaaagtaat gaagatttaa
     1801 ttgaagtata tgtagattca atcttattca gaagggctat aaccaatctt atatttaatt
     1861 caatagttca taatagcgaa ggaactttga taagtgtaga aattgtaaaa aaagataata
     1921 tagaaattat aataaaagat aatggaattg gaataagcaa aagtgatttg aaacatattt
     1981 ttaaaaaata ttatagaggt actaatacag gtgagatgca taaaggttca ggtcttggta
     2041 tggctatttc taaagaaata atagagattc ataaaggtaa aatttatgta agtagtgaaa
     2101 ttggaatagg aacaaaaatt attatagaga taaaacaaaa ttaggagata tgatatggat
     2161 ttttttaaaa aaggtataag tgggtttact attaagattt tagcattaat atttatgaca
     2221 tttgaccata tagcagcatt tatgccacaa acaatgcaaa taccaatttg gtttcattgg
     2281 gtaggcagaa tatcagcacc actgtttata ttcatggctg ttgaaggatt ttatcacact
     2341 agtaacagaa aaaaatatat aagtagattg tatatttggt ctgttattat ggctatagga
     2401 aatcaaataa ttaataatgt attttcacat ccagaaggag ctataattat aaataacatt
     2461 ttttcaacat tatttttaat agcgatttat ttacaagcaa tagagttcat aaagaaattt
     2521 agaaaagaga aagaaattaa atattttatt ataggattat tgatgataat cattcctata
     2581 attttaggaa tatttacagt agctttactt tttaaagtca ctaacagagt tattacttta
     2641 tttatgattt tggtaccagt accattttta gtagaaggtg gaccaatttg gattatactt
     2701 gggattattt tttatttgtg tagaggtaaa aagttcagtt tgagtatttg ttatgttttg
     2761 atgtgtatat ttatatttac aacaatgtca aatggagatt atagtttaaa aaattctatt
     2821 ttacaaaatt accaatggat gatgattgct tctctacctt taatgttact ttacaatgga
     2881 gaaaaaggga agagtatgaa gtatttattt tatttatact atccaatcca tgtatatata
     2941 ttgtatatct taggaatata cttaataaat ggtttttgat tgtaagtgat atttttaata
     3001 caaaatgaac aatatgagga ttgttttagg tctattaaaa ttgttattta gatgagattt
     3061 gtagaatatt taaatttatg agcagaaatt cagttattgt aatttcaata acattagaat
     3121 ataatattat
//
LOCUS       DAEPOG010000271         3073 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEPOG010000271 DAEPOG010000000
VERSION     DAEPOG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.274"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(145..666)
                     /locus_tag="KR432_003560"
     CDS             complement(145..666)
                     /locus_tag="KR432_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0642064.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="KR432_003561"
     CDS             794..2158
                     /locus_tag="KR432_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0642065.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="KR432_003562"
     CDS             2347..3024
                     /locus_tag="KR432_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0642066.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT          959 a          439 c          497 g         1178 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgt
//
LOCUS       DAEPOG010000272         3022 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEPOG010000272 DAEPOG010000000
VERSION     DAEPOG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.275"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            335..2146
                     /locus_tag="KR432_003563"
     CDS             335..2146
                     /locus_tag="KR432_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0642067.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2234..2815)
                     /locus_tag="KR432_003564"
     CDS             complement(2234..2815)
                     /locus_tag="KR432_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642068.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1131 a          303 c          423 g         1165 t
ORIGIN      
        1 tatagattat gtatatgcca aatttaaaaa gagacataaa gacattatac aaatatatta
       61 ttggggtgat attttataag aaaagaagtg atgcagtaaa ttaattgagc aatttaaaaa
      121 tgatttaatg atagtgttga tgaatgttta aaaagaaatt ttagaaaaac gtatattgct
      181 gtttgtgttg gaggtgatga atttgtataa tttatagggt ataaatgatt aaactactta
      241 agataaatac atatcactgt aaaatattga taatcatttt ttataagaga ttgattattt
      301 gcaaattata tatattacaa atgaggtaac aataatgaag aaaaaatatg attttttaat
      361 ttattctgct atttggctag ttattattat tttttttata ttttctctat atcatagtat
      421 agagcatatt aaaattataa gtcacactgg tactattcgc agtgaaactc aaaaggttgt
      481 taagcaagaa ttgaataatg aaagaaatga tgacttgata aaacgactgg ataatattct
      541 gataaagcta agaacaggta atggagaaaa tggatttcaa agatgtgata ataaagaatt
      601 tcaacaaaaa ctaaatcaaa tggattctat gtgggaatct atgaagaaag aaattataaa
      661 agttagaaat ggtgcttcag gtgataaatt atataaattg agtgaagagt attctgtgtt
      721 gtctaatcaa attgttttta tatctgaaaa acactctaat gcaaaattgt attcttttgc
      781 tactgcacta tttatttatc ttatattctc aacaataagt cttttaattt gggaatatta
      841 taataaaaaa aggtttaaaa gaatatttta tactgataat ttaacaaaaa taaaaaatca
      901 ggttgcattt gaaaacagag cgattgaaat tttgtacaat gcatctaaca aagagtatgt
      961 actgctaaat attgatattg acaattttaa atatattaat gatacacatg gatatgaata
     1021 tggagataaa gttctaataa tagttgcagc tgcactttct aaaactttta atatcaaaga
     1081 aacttgtgca agaattggtt cagataattt tgtaatactt gcaaaatata gagattctct
     1141 tttagaggat attcgggaaa tgctaactaa tgctataatc tctgaattag atatgaatgt
     1201 aactcaaact atatcttatt gtataggagc gtacttagtt gaaattgata atttaggtta
     1261 taaatccatc aattctataa tggataaggc aaatattgca cacaaggtaa gtaaaacaag
     1321 aggaatttcc tccacagtat ggtataatga aaatctgtta aaacagttac aaatggaaaa
     1381 tagtatttat aattatatgt ataaagcgat agaaaatgaa gaatttcata tgtatttgca
     1441 acctaaattt caaatatctt ctttaaatgt tgtaagcgca gaagctcttg taagatggtt
     1501 ttctccagaa cttggttttt tatcaccaga tgaatttatt ccattgtttg aaaaaagtgg
     1561 atttattatt gaattggact tttatatgtt aaaaaaagcg tgttcatttg taaaaaaaac
     1621 gtttatgaaa aaaaatcaat atacatatcc gattgcagtt aatttttcaa gggttactat
     1681 ttaccaaaat agcttttatc aaaggttttt agatactgtt aaagaatatg aaattccttt
     1741 taagtatata gagatagagg taactgaaag tgcttttaat gaaatatctc aaccagttat
     1801 ttcgatttta gaggagctta aaaagttagg atttttaatt tcgatggatg attttggatc
     1861 aggatactct tcattaagtc ttttatgttc tctatctatt aatggattga agttagataa
     1921 aagcttactt aaagaaacat ttaaccggga gaaagtatat agtattattc agtgtattat
     1981 tgaaatgtca catcgtatag gcatgtctgt tgtatgtgaa ggtattgaaa ctaaaaaaga
     2041 cttggaattt ttaaatacag ttaaatgtga tgtaggacaa gggttttatt tctctaaacc
     2101 gatagaagaa aaagaatttt ttaacaaata tgtaactaaa aaataatata attattttta
     2161 tcagtctata cattgagtca ttttatattt cgttaaatga ctcaatgtat tgttatgtat
     2221 tttaaaatat aaattattta aaacgttttt tgtacttata tagaaattct tctatatttt
     2281 ttgattgttc tcttatttca actgcttcta tatctagtaa ataaagtata gaatcatttg
     2341 taagttcaac atattcacca tcttctgaac cgagtgcatg tctctcaatt tttaatcctt
     2401 tttcttcata atattttttt gtttctatat ataatgattc tcgcttttgt atgtagtctt
     2461 tcacttcatt atttggaaag ttatatgaat ccactaatgt ttcgccattt ataaaagttt
     2521 tctcggtagc aactttttca tcccaataat ggatttcttc ccaagtaaaa aatatctcag
     2581 ttccatctgg aatgaatgaa aacctggtaa taatatataa ataattattg aaatttactt
     2641 cataatattc aggaatatct tcaaattcca tatagtaatt tgtataatta aaattactat
     2701 tatctttttt tacaaagggt attttttcaa taaatttata tattgattgt tcatttttaa
     2761 aaattcctat atcatagctt ctttcaaatt gattaatttt taataaatac aacattcttt
     2821 ctccttttat tcaaaatata atttttataa atttactaat ttttagcttt tttaattata
     2881 tatttacata tgtttatatg atactataat gatgtgtttt aaatagatat ataatagtaa
     2941 atatttttta tatagttatg ttttagtaaa ataatgagat aaatatatat aaagcttaat
     3001 ttttgaactt atggttttgt tt
//
LOCUS       DAEPOG010000273         2886 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEPOG010000273 DAEPOG010000000
VERSION     DAEPOG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.276"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            259..1308
                     /locus_tag="KR432_003565"
     CDS             259..1308
                     /locus_tag="KR432_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0642069.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1458..1805)
                     /locus_tag="KR432_003566"
     CDS             complement(1458..1805)
                     /locus_tag="KR432_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0642070.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(2262..>2886)
                     /locus_tag="KR432_003567"
     CDS             complement(2262..>2886)
                     /locus_tag="KR432_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH0642071.1"
                     /translation="AGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT         1061 a          383 c          392 g         1050 t
ORIGIN      
        1 tatgatttat attattaaaa aatttttcct tatatataat agacaaaaaa gtgatattat
       61 tttaatttta ttacttagtt tataaataat caatataatt tatccaatat tacatattac
      121 taaaaaaact aaacataaaa ctgaaaatta ttattaagtt tttgtagaaa tattataagc
      181 atagatacat ttttgtaaca actacaataa atttttaata ctatatacta ttcgtatgtc
      241 ttatgggagg tatttattat gtttaaaaaa atagtcgcaa tagcaagctc tatcattgta
      301 agtacaatat gtctaactgg atgtgatttt aactctaatg acaaaaaaga tgaatcttct
      361 tcgtctaaaa atctggataa agtaactata gcagaagtta ctcactctgt tttttatgct
      421 ccacagtatg ctgctattac gaaaggattc tttgaagatg aaggtataaa gattgacctt
      481 ataaataccc aaggtgcaga taaaactatg gcagctttga tttcaggtga agcaaacgta
      541 ggtttaatgg gtcctgaagc atctatatat gtatataatc aaaacaaaaa cgattatgca
      601 gtgaactttg ctcaattgac aaaaagagat ggtagttttc tagttgctag gaaaaaaatg
      661 cctaatttct cttacaatga tttaaaagga acagaaatat taggtggtag aaaaggtgga
      721 gttccactaa tgacttttga atatgttcta aaagaaaaag gtcttactat tggagaaaat
      781 ctaaaagcag gcaatgttaa tgtaagaact gatgtacaat ttggtgtaat ggctggagct
      841 tttgcaggtg gagagggtga ctttacaaca gcatttgaac ctacagcaac agcaatggaa
      901 aaagaaggta gtggatatat agttacatct ataggaaaag attctggaga aatacctttt
      961 acagcatact ctactacgag atcatacatg aaaaacaata aagatttaat tcaaagattt
     1021 acaaatgctg tttataaagg tcaattatgg gttcaacaag catctagcaa agaagtagct
     1081 aaggctatgc aacctttctt tgaagatata tctttagaag atttaacaac agttgtagat
     1141 agatataaat caatagatgc atggtctcaa aatcctgtat taaaggagga aggtttgaag
     1201 aaattgatga ctgttatgaa agaagctaaa gaactagata aggaagcacc ttataaagat
     1261 atcgtaaata ctgagtttgc aaacaatgct ataaaaaata ttaaataaaa tatattagtt
     1321 ttagctagat attttcttgt ttttataatg aaaattaaca aaaatataaa aagagccttt
     1381 taaaagactc tttttatatt tgagattaaa gtttatataa taaaatagat attaaagtac
     1441 taactgtact tatatatcta tcctctgtat tctgctccag cattaatttt tgtaatttga
     1501 acggctacca tttcttcatt gcttccatta gtaatttcat gccactctcc tgttggtaca
     1561 aatacagtat cgccgttttt tacatcacat tcatgctcac caagtttcat tttaccttct
     1621 ccttttagga acacaaatat ttgttcacca ttagggtgtc tatgagcatt aagaacttga
     1681 ttaggtttaa aataataaac ttctgaagtt atatcgttac cagcaaatac tggtattttt
     1741 ggaactgctg tatcattata ttgagctaag cttccgtcta atagattctt tttattttca
     1801 tgcatactag aaatacctct tttctatatt atagatttac tattattata ccaatataag
     1861 aaaatagtat tctcacttta cacaatagga ataaaattct tttattttta aatataagta
     1921 aatactattt ttatagttag aaaaatataa tcattataag atataaaaaa attaacataa
     1981 tataataaat tctaaaagta tttttagata tctaaacttt aatataaaga atatatgaat
     2041 attttttata aaaaattaaa attatttaat acatttaaaa tttaattaga ataaataatt
     2101 ttaatgaatt aaaaaggacc acttcattta ataactagta ttacgaatgt ggtccatatc
     2161 aaagatagaa aatttattaa tatttataaa atattttact catatgtgaa tttatttttt
     2221 atttcagttt aatttaaggt atacaaaatt attttataaa atcaacacca aaagtcattt
     2281 ttacaacaat tacagcaaca atctttacaa ccacaatcat tgcaacaaca attactacag
     2341 ttgttacaac agcaatcatt acaacaacat tgttttattt ctacttgcac tcttgatata
     2401 ttattgttaa ggtttgaatc tggtgttgtt gaagtaactt ctgcaatatt tgttaatcta
     2461 tcaagacatg ttgattttat aattcctctt aatagaataa ctcttaaagt tccagctggt
     2521 agtgtaccta tattaagact tccagtccat ggttggaaac ttacacctct atcaagtgaa
     2581 aatactgctt tcttaagctt atctgaaaca ttgtctatta aagtaacatt ttgagcattt
     2641 tctggtcctg catttgatac tactattaca aaatctattt gctcttctac acatgcttct
     2701 gttttatttg ctaattttat tacagaaata tctgctgatg aacttatatc tatttctgat
     2761 gttgaagtat tgttatttag atttgggtca ggtgtagttg aacttactgt tgctgtattt
     2821 ataattgtac ctattgctgt ttgacttact attcccctta ttatgatatt tcttatctct
     2881 ccagca
//
LOCUS       DAEPOG010000274         2870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEPOG010000274 DAEPOG010000000
VERSION     DAEPOG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.277"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            56..1681
                     /locus_tag="KR432_003568"
     CDS             56..1681
                     /locus_tag="KR432_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0642072.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            1880..2356
                     /locus_tag="KR432_003569"
     CDS             1880..2356
                     /locus_tag="KR432_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642073.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2564..>2870)
                     /locus_tag="KR432_003570"
     CDS             complement(2564..>2870)
                     /locus_tag="KR432_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0642074.1"
                     /translation="SIRNNNNLLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDL
                     RHSHVTLLINSKVPIKVISERVGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
BASE COUNT         1140 a          298 c          522 g          910 t
ORIGIN      
        1 tatcaaagtt ttatgataaa ataaattatg agcaaaaaga aaggaaaata tattaatgtt
       61 acaagttaca ggtgttggac ttagatttgg tgataaggaa ctatttaaag atgttaactt
      121 aaaattcact aaaggtaatt gctatggaat aataggagct aatggtgcgg gaaaatctac
      181 ttttttaaaa atattatcag gagaaataga gcctaataca ggtagtatat ctataactga
      241 taaagaaaga atgtcagttt taaaacagga ccactttgaa tatgaagaag aaactgtttt
      301 aaatgttgtt ataagaggtc atgagagact ttggaacata atgaaagaaa aagatgcatt
      361 gtatatgaag gaagacttta atgaagaaga tgggataaaa gctgccgaac ttgaaggtga
      421 gtttgcagag cttgacggat gggatgcaga aactaatgct gagaaaatac ttatgggtct
      481 tggcataacg aaagatatgc attacaaaca aatgaaggaa ctagttggtg gggaaaaggt
      541 taaggtattg ttagcacaat cactttttgg taagcctgaa atactattaa tggacgaacc
      601 tacaaaccac ttagatttta aatcaataaa ctggttaaat aattttataa tggatttaga
      661 agagtctata gttataatag tatcacatga tagacatttc ctaaatcaaa tatgtactaa
      721 tatagttgac gtagattttg gtaaaataca aatgtacgtt ggaaactatg atttctggta
      781 tgagtcaagt caattggctt tacaactggc taaggaccaa aataaaaaga ctgaagaaaa
      841 gatagctcaa ttaaaggaat ttatagctag atttagttct aatgcttcaa aggctaaaca
      901 agcaacatca agaaagaaac agttagagaa attagaagtt gaagatatac aaccatcaag
      961 aagaagatat ccatatgtag gatttacacc tgcaagagaa attgggaatg aagttttaga
     1021 agtacacaat ttaactaaaa ctatagatgg ggttaaggtt cttgataatg tgtcatttag
     1081 attggataga gatgataaag tagtatttat gggagatgaa atagctacta ctgcactttt
     1141 caacatagtt atgggagaac ttgaaccaga cagtggagag tataaatggg gagtaactac
     1201 ttctcaagat tatcttccaa aaaatcataa taagtttttt gacggagtag aatattcttt
     1261 ggttgactgg cttagacagt tctctgaaga aaaaagtgaa agttttataa gaggattttt
     1321 aggtagaatg ttattctcag gtgaagaagc attaaaagaa gctcaagtta tatctggagg
     1381 agaaaaggtt agatgtatgc ttagtaagct aatgctttca aatgcaaatg tacttgtatt
     1441 agatgaccct actaaccact tagacctaga aagtataact tctgtaaata aaggacttga
     1501 aaagttccca ggagtgcttt tatttacttc tcatgaccat gaatttatat ctactatagc
     1561 aaatagaatt atagagataa ctccaaatgg aataatggat agaaaaatgg actttgatga
     1621 gtatttagaa agtaaagaga tacaagacca attagctaaa atgtatggaa aagataaata
     1681 attattagta taaaaccttt taatatttga tacatgctat gcatgagtgt gtaaattttt
     1741 aatcaaatat taggaggttt tttatgttgt ttaaatatgt ttattagcag aatcgagtaa
     1801 tattttgtat attaagaatt taaatataat atagtagatg atataataaa aatatataac
     1861 attttggggg gaatagatta tggttaagaa aaggttagta gctgtagtat tgagtttagc
     1921 tatgattagt gtagggaatt tttcatatgc tcactcagga gggcataatt catcaggagg
     1981 tcatcataat aaaaataaat cagagttaac acgttattat tgtggaggac attcagcaca
     2041 tcaaccaaat aaaggttttt gtccttatgc tagtacaaaa gaaaattctg gagtaacaat
     2101 atcttcagta cataacgaaa aaaatcataa aaactatttt gcagaaaaag ggtataataa
     2161 aggttatgaa gatgggtgta aagggcttta taatttaagt gtcacagaag ccaaatctga
     2221 taatttatat gaaaaagaga ttttaaaaag ttcatatgaa aaaggatatt taattggata
     2281 caatgaatat aaagcaaaaa aagtagatga tgtaatgaag aagatgttag atattccaat
     2341 gttagcaagt atataataaa atagtattta aaaagatact ttctataagg aaatatcttt
     2401 tttattgtaa agtataattt ttcataaaaa agtataataa ctgaggcgat aaattaaaat
     2461 aaaacagatt atttaagtag ttattaattt gagatagata catttaatat taatgaatct
     2521 atctcaataa gctaagctaa tttaaagcta agataaatta atattaatta gcattaaata
     2581 aactttcaga aattttgtca gaagcctctt tatccatttc tttaagtaca tgagaataaa
     2641 cgcttagagt agtacttata tttgagtgtc ctactctctc ggatataacc tttataggta
     2701 ctttagaatt tataagtaaa gtaacatgtg agtgtcttaa atcatggaat ctaatatgag
     2761 gtaattcatt cttttctaaa aatcttttaa attttttact cataacatct tcagcaatag
     2821 gttcacattt cttattgaaa aacaacaaat tattattatt tctaatggaa
//
LOCUS       DAEPOG010000275         2838 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEPOG010000275 DAEPOG010000000
VERSION     DAEPOG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.278"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            164..1408
                     /locus_tag="KR432_003571"
     CDS             164..1408
                     /locus_tag="KR432_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642075.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            1605..2474
                     /locus_tag="KR432_003572"
     CDS             1605..2474
                     /locus_tag="KR432_003572"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH0642076.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(2528..2716)
                     /locus_tag="KR432_003573"
     CDS             complement(2528..2716)
                     /locus_tag="KR432_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0642077.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
BASE COUNT         1170 a          339 c          404 g          925 t
ORIGIN      
        1 aatctaatgc taaattttga cataataata acatatttat ttctataata aaaaattcac
       61 aaattataaa aaataaaatt ttcaaaaaaa ttataatttt caaaaaaaca aattgacaca
      121 tataaaaaaa ttatgatacc ctaataaaaa gggggcaatg attatggaaa acaatttaaa
      181 tatagctaaa aaaaataata ctgaattatt acaaaatgac tcacttaagg tcaacttaca
      241 gataggtgta gactttacac ttttatcaaa atcacttaaa gccttttata aaaaagaaaa
      301 agatttttat tgcattgctc ttgcgccatg caatgtatct agtgaaaaag caaatagaaa
      361 aataagtatt tacgatatga taaaagaaat taatatttta attgaaagta taactgataa
      421 aaatgaactt ttaagtgaag aactctttac taataattta tcagaatttt atgatgaatc
      481 tttagatgag atttatatag acttaaaaca agcatattta tatattaaaa agaatacaga
      541 tagtcttaac atagaagact caattgaata tgtatttgat gtagatataa gcaatgatat
      601 aaaagcaaag gaaaatccac taattaattt taattcactt tctttagtag tatggagtgg
      661 cgaaacaaaa catattttac aacaaatgaa tattttagac ttagaggata ttcactctta
      721 ttatatggaa aataatacaa atataactat ctatgaaaat aatactcttc aatataaatt
      781 tgatgaagaa tctgaagata taaaagacag tagcatagat ataattgaac aaatgcttat
      841 acttcctgat gacttgaatt atgataaaga tgaagttgaa aatatgaaaa gcagacttgc
      901 aaaaataaat attaaatatt tacagacact taaagaaaag gatataaaaa taaaattaat
      961 aaattcaaat ctaactgatg aaccagaatt tagtgattta aaatatcaat tacctccatg
     1021 ttgggtaaga tcaggcaaga cttggaaaga tgtacctggt atatatagaa ataattctat
     1081 agtagctaaa ataggttata gtaaccctag ctatgcaaat gtacattcat caaaaaattt
     1141 agaattacat gaaactgcac atgctataga taaaaatgtt ttaaataaaa aatcaaattc
     1201 tgaagaattt atggaagtat ttgctcagga aagatataag ctttatgacc ctaaacaagt
     1261 tgcacatgca tatataagca agtttataga agaatttttt gcagaatcat ttgtgcatta
     1321 ttatcttgat gaagattcaa aaaatacttt aaaggaaaat tgtcctctaa catatgattt
     1381 tcttgaaaaa cttgaactaa actattaagt gcgctaatat ccaataatac atttaactat
     1441 cctcttttca tcaacaataa ccatataata atttatactt tgaataaatc actatctata
     1501 aaggatttct tagtatttat gttataatat ttataatggt tatgttttaa aatagtttat
     1561 gtaacccata atttaaataa ataataaaag aaggatgaaa agttatgaag ttaaatagaa
     1621 atgatagctg ttggtgtgga agtactaaaa aatataaaaa atgtcatatg gatatggata
     1681 ataaaataga ttcatttgct aagttaggtc atattgtacc aactcatgat attataaaaa
     1741 atgaaagtca aattgaggaa ataaaaaaaa gtgctgtaat aaacacaaaa attttggatt
     1801 atatttcaga taaaatcgaa attggaatgt ctacagaaga tatgaataag ttggttcatg
     1861 actttacact acaaaatgga gctatccctg ctccactaga ctataatgga tttcctaaaa
     1921 gtgtatgtac atcagtaaat aatgaagttt gtcatggtat tccaagcaag gacattatct
     1981 taaaagatgg agatattata aatgtagatg tttctactat ttttaatgga tactattcag
     2041 atgcttcaag aatgtttatg ataggtaatg ttaatgaaaa tgcaaaaaaa ttagttcaag
     2101 ttacaaagga atgtgtccaa aaaggtcttg aagttgccaa accatgggga tttttaggag
     2161 atataggtga tgctatcaac tcttatgcaa agaaaaatgg atactctgtt gttagagaaa
     2221 taggtggtca tggagttgga ttaagctttc atgaagaccc ttttgtaagt tatgtatcta
     2281 aaaaagggac tgagatgcta ttggttcctg ggatgatatt cacaatagaa cctatggtaa
     2341 atgaaggaac tcacgaaatt tttgttgatg aagaaaataa ttggactatt tatacaaaag
     2401 atggtaaatt atctgctcaa tgggaatgta tggttttagt tacagaaaca ggggttgaaa
     2461 tactaactga ataaaagaca aatgcctata aaaattgaaa attcaatttt tataggcatt
     2521 tattttatta atgcacatct attgcatcaa ctggacagct ttctctagct tctgctgctg
     2581 attcttcagc atcacttggt acttcatcaa ctataacatg tgatttccca tcatccttca
     2641 tatcaaatac ttctggacat attgatggac ataatccaca acctatacaa gtatcttgat
     2701 taacatttgc tttcataata aaatcctcct agacgctaca aatttaaaat cttgtttatc
     2761 aaagattgat gaaccaaaac tgtaacattc agttcttttt tattatgtcc tcaaatttga
     2821 aaaaaatacc ttttaaaa
//
LOCUS       DAEPOG010000276         2817 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEPOG010000276 DAEPOG010000000
VERSION     DAEPOG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.279"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            135..1355
                     /locus_tag="KR432_003574"
     CDS             135..1355
                     /locus_tag="KR432_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0642078.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1392..2741
                     /locus_tag="KR432_003575"
     CDS             1392..2741
                     /locus_tag="KR432_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0642079.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         1074 a          353 c          463 g          927 t
ORIGIN      
        1 tatactatac atatagataa atgattatat ttattcaata ttcttataaa aaacaaaatt
       61 ctccatcgaa aattatggca taataattgc ttattatatt aataaaacaa taaaaagcaa
      121 ttggaggaat aaaaatgcaa caggctttga aattaaaata tcaaaccaaa gaattaggaa
      181 aagattttgt aatagaaagt tgtaattcta taaaaccatg gcttataaac ataagaagag
      241 aattacataa aattccagaa ttagctcttg aagaaaatct tactaaacag aaggtaattt
      301 cttatttaaa agaaatcgga attgattata tggaatttac aaaacacaat ggaattatgg
      361 catatatttt aaaagaaagt gcagataaaa cgatttgtat aagagcagat atggatgctc
      421 ttcctattga agaagaaaac aacatacctt ataaatcaat acattctgga aaaatgcatg
      481 cttgtggtca cgatgcacat acaaccatgc tacttggagc atgtaaagtt ttacattcaa
      541 taaaagataa acttaatgta aatgtaaaat tcttatttca accagcagaa gaaggttttg
      601 gaggagcaaa atttttagtt gaggatgggt gtttagaaaa ccccaaggcc gattatatat
      661 tcggacttca tgttatgcca catatagaaa caggccttat cgaaacaaaa tatgatacat
      721 taaatgctag tgtagacact ataaaaataa gtatcaaagg taaaagagct catggtgcat
      781 atcctgaaaa tggtattgat gcaatagtta cagcttcaca aattgttaca tcattacaaa
      841 ctataataag tagaaactta gaaccaaata atgctgccgt tttgactata ggtaaaatat
      901 atggtggtga tgcacacaat gtaatatgtg aagatgtaaa actagaaggc actttaagaa
      961 ccttaaatag caaaacaaga aactttatga ttgataaaat agctaaaatt gttggagaca
     1021 ctgcatctgc atttggttgt gttgggactt tacatgtaag tgatgaaaac tatcctgctg
     1081 ttataaatga aaaagaatta gtagatacag ttatatcaag tactaaagaa ttacttggag
     1141 aggaaaaatt tatattgaga ccaaacccgt ctttaggtgg tgaagacttt tctttttaca
     1201 cagaacattg taaaggagca ttcttccatt taggatgtaa aaatgaggaa aagggcttaa
     1261 tttcacctct tcatacctct agcttcaata tagatgaaga ttgtttacca ataggtgtta
     1321 tgatgcatgt aatgaatact ttgtatttta attagtacag taattaataa aagaatagga
     1381 gataaattaa tatggaaaaa gctaagaaat taggtctatt tagtatgata ttattaggga
     1441 ttaatagcat aataggttca ggtatattct tactaccagg taaagtttat agtctagcag
     1501 gtcaaaacag tatgtttata tacatttttg ctacactctt agttctatca atacttttat
     1561 gttttgcaga agttggaagc atgtttgata agaatggagg agcatatcta tactccaaaa
     1621 aagcttttgg agattttata ggatttgaag ttggtacaat gtcttggttt attagaatta
     1681 tatcatggtc tactctagca gtaggatttg caacagcttt aggttcattt tggccagaat
     1741 ctgccactga atacaaaggt tgtatagctg caatactcgt aacactactt tcaataaata
     1801 gcttatttgg aattaaaagc acaaagataa tgaataatgt aataactata gctaaattag
     1861 taccattaat agtttttgtg atagttggaa tattctttat aaaatttgta aatattgttc
     1921 cttcaggaaa tgtagttaac tctagtatgg gtccagccat tatattagta ttttacgcat
     1981 ttaccggctt tgaatcattt attgtggcaa gtggagaaat ggaaaatcct aaaaaaaatc
     2041 ttccaatcgc attaataacc acaatattta tatgtgcaat aatttatata ttaattcaaa
     2101 ttgtctgtat gggtatactt ggagatagac tatttgaaaa cagtattcca attgcagatg
     2161 cttcaagtgt atttttaggc aattatggta aagttttcat atctgtagca acattaatat
     2221 ctatatttgg tataaatata ggttcatcaa tagtaactcc taaatgtggt tcttctctag
     2281 cagaagaagg ttctcttcct gcatttatag gaaaaacaaa taaatatggt gctccatatg
     2341 tagcaataat aatatcttta atttgttgta taccacttgt gctaactgga agttttgaac
     2401 aacttgcagt aatgagtgta attgcaagat ttgctcagta cataccaact tgtttatcag
     2461 taatagtttt aagaaaaaga acagatgtta aggcctcttt taagattcca tttggtcctg
     2521 ttataccaat ggtagctatt ttaggtagct tatggttgtt acaacaagct tgggcagaag
     2581 atatttcaaa acctataact caaaatagag tcttaatagg attaggggca atgttgttaa
     2641 tagctcctct atatatattt atgaaaaaaa ataaggaaat cgagcataaa aataaatcag
     2701 aagattctaa attatatatt tcgagagaaa aaattcaata atattttata ctattacttc
     2761 tcataaaata taataaatat aaaaaaattg tagataaaac tattttataa aatttat
//
LOCUS       DAEPOG010000277         2782 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEPOG010000277 DAEPOG010000000
VERSION     DAEPOG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.280"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            23..325
                     /locus_tag="KR432_003576"
     CDS             23..325
                     /locus_tag="KR432_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBH0642080.1"
                     /translation="MKKYQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIED
                     ARYVFPNACETKMVFTINARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            338..1075
                     /gene="rlmB"
                     /locus_tag="KR432_003577"
     CDS             338..1075
                     /gene="rlmB"
                     /locus_tag="KR432_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH0642081.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            1078..1605
                     /locus_tag="KR432_003578"
     CDS             1078..1605
                     /locus_tag="KR432_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBH0642082.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            1670..2317
                     /gene="sigH"
                     /locus_tag="KR432_003579"
     CDS             1670..2317
                     /gene="sigH"
                     /locus_tag="KR432_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBH0642083.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            2391..>2782
                     /locus_tag="KR432_003580"
     CDS             2391..>2782
                     /locus_tag="KR432_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0642084.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT         1181 a          319 c          543 g          739 t
ORIGIN      
        1 aaaagagtta tttataaatt caatgaaaaa ataccaagag gattatgata aattagtaga
       61 gatactattt gaaaatcatt ataacaaact aataaaaggt ggtaaaaacg agaaaacatc
      121 aaaaagacaa gttgagaaga aagcaataga agatgctaga tacgtatttc caaatgcgtg
      181 tgaaacaaag atggtattta ctataaatgc tagaagtttg tttaatttct ttgaacatag
      241 atgttatgaa aatgggagtt gtctagaagg aactatgact tgtggagata tagttcaagt
      301 tagaaaaaaa tttaagaact tgtaggaggt gaataatttg gcgagtatag aaggaagaaa
      361 tccggtaata gaagcaataa aaagtgacag agaaatagat aaaatattaa ttgcaaattc
      421 agctaaagaa ggttcaatta agaaaataat aggaatggct aaagataaga atataattat
      481 tcagtatgtt gataaacata agctagatga agtaagtaca agtcattcac atcaaggtgt
      541 aatagcttat gcaagtgagt ataagtatta tgaattagat gagctaatag atttagcaaa
      601 gaataaagat gaagacccat ttttcattat attagatgag ataacagacc cacataattt
      661 gggttcaata ataaggacag cagatgcagt aggagcacat ggagttatca ttccaaaaag
      721 aaggtctgta cacataactc ctgtagttgc caaagcatct gctggagcag tagaatatat
      781 gccagtttgc aaggtaacca atatagttaa tacaataaaa agactcaaag aagaagggct
      841 gtggattgct gcagctgata tggatggaga aactttttat aaacaaaatc ttactggtcc
      901 tcttggtgta gtaattggaa gtgaaggttt tggaatatct agattggtta aacaaaactg
      961 tgactttata gtaaaaatgc caatgatagg taatgttaca tctttaaatg catctgtagc
     1021 aggtggaata cttttatatg agatattcag acaaagactg gataaaagta aataggcatg
     1081 aaaaaaaata taaatcatta cttaataata gatggctaca atattataaa tgcgtgggac
     1141 aatttgaaag aactggctaa agaggactta gaagattctc gagaaaagct tattgatgac
     1201 attatagaat tttctgagtt tatgggttat aagactataa tagtttttga tgcttataat
     1261 gttaaaaatt ctcgagaaaa agttgaaaaa agaaaacaca taacaatagt ttatacaaga
     1321 gaacaccaaa cagcagatag ttatatagag aaatttataa cttctttatc aaagtatgat
     1381 gatgtaaaag tagccactaa tgactatgca gaacagcaaa tgattttagg aaaaggtgct
     1441 actagaatgt ctgcaagaga attaaagtta gagttagatc gttccaagaa taaaatgaag
     1501 gaaaaaaata tcggtttgag gaaaaaaatt cagcgaaatt ggttggagga aaggttagat
     1561 aaagaaacat tgtcgaaact tgagaacatt cgtagaaagc gttgaaatta ctaggttctt
     1621 tagactataa tatacctata aatttgtttt atatatgggg gagatttaca tgttggtagc
     1681 aaaagaaaaa agttatgagt tagtggacaa ttgtcagcag gatgagtata acattgtatt
     1741 aagagcaagt gaaggggata aaatagcact agagtacatt attactaaat acagaaattt
     1801 tgtaaaggca aaagcaaagt catatttttt aattggagca gataaagaag atataataca
     1861 agaaggaatg ataggccttt ataaggcagt tagagatttt gatggaagta agaccaattc
     1921 atttaaatgt tttgcggaga tatgtataac aagacaaata ataactgcta taaaaactgc
     1981 aacaagacaa aaacatatac ctttaaattc atatgtttct ttgaataaac caatatatga
     2041 tgaagagtca gacagaacac ttttagatat tatagctaca agtatagtaa cagatccaga
     2101 agagcttata attagtaaag aagagttaaa aaatatagaa tcaaaaatga acgaattgtt
     2161 aagtgacttg gaattagaag tactagaatt atatttaaat ggaaagtcat accagtttat
     2221 agcggacaag cttaaaagag atgtaaagtc aatagataac gctttacaaa gggttaagag
     2281 aaaattagaa aaacatcttg aaaacagaaa tgattaatag taaaatactt accataagct
     2341 tattgtgagc aaaattaatt tatatcgaaa gataagggag gaaattcaaa atggctaaag
     2401 ctaaatacga aagaacaaaa cctcatgtta atatagggac aataggacac gtagaccacg
     2461 gtaaaactac attaacagca gcaataacaa aaacattata tgacagatat caattaggag
     2521 aagcagtaga tttcgcaaac atagataaag ctccagaaga aagagaaaga ggaatcacaa
     2581 tatcaacagc acacgttgag tatgaaacac caaatagaca ctatgcacac gttgactgcc
     2641 caggacatgc tgactacgtt aagaacatga taacaggagc agcacaaatg gacggagcaa
     2701 tattagtttg ttcagcaaca gatggaccaa tgccacaaac aagagagcat atactattat
     2761 caagacaagt tggagtacca ta
//
LOCUS       DAEPOG010000278         2765 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEPOG010000278 DAEPOG010000000
VERSION     DAEPOG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.281"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..756)
                     /locus_tag="KR432_003581"
     CDS             complement(<1..756)
                     /locus_tag="KR432_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0642085.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFT"
     gene            complement(762..1649)
                     /locus_tag="KR432_003582"
     CDS             complement(762..1649)
                     /locus_tag="KR432_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642086.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(1663..2685)
                     /locus_tag="KR432_003583"
     CDS             complement(1663..2685)
                     /locus_tag="KR432_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642087.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT          970 a          469 c          247 g         1079 t
ORIGIN      
        1 tgtaaaattt tcatatgtta ttttagttaa cactatcatt aatataattg agattactat
       61 agaaaaaact atcaaaatac ttggtacatt atacctaacc atttgttggc tcatcataat
      121 ccaatttgga gtcactgaca taaattttat agctttatct ggtataaaac ctacactaaa
      181 gtatggaatt gccattatca atccacctag tataaatgga attacagccg atttaaatat
      241 agatgaaagt gttaagccta tttgtgcaaa tataaaagaa gcaaaaatag tagtcaatat
      301 aacaattata tacatttgta ttactgtaag atttaaatta ctatttccaa agtaccataa
      361 gttatgaatc ggttcattta aatttttaaa atcaaaaaaa ctaaaacctc taattcctac
      421 taacaatata aaagaaatat taataataga taaaattaat ccattagcta ctagttttga
      481 taatactact gatttttttc cctttttagt tgtatttatt aaacctgtca tattagaatc
      541 atattcttta atgtaatttc cagaagttat tataaacatt atcaaaccta ttagaggtat
      601 tgagccttct atcttgtaaa atatatctgc ataagtatct aaaacatcaa actttctaat
      661 cagaagaaat cccaaaaata ttaaaaacag ccaaattatc caaacactct ttgattttaa
      721 tatttttatc atttcagctt taactaaata catcatttct actacacctc ccttgcatct
      781 tccaaatcaa aataaaacat gtatacatct tcgaattttg cttgaactgg ttgtatatta
      841 tccccacttg gaatatcctc tgaaacaatt ctaagtacta ctgaatcaat atcactttga
      901 atacttacaa ccttatattt agattgtata tcatcaactt cacattcatt tttaacttga
      961 acattatata ctttattttc catgtgactt aatatgtctt tgtgtgttcc tttcattaga
     1021 agtttgccat ctttaatcat tattgtttcc tttgcaactg attctatatc ggaaattata
     1081 tgagtagaaa gtattattat cttatctcta gacatttttg ctattaaatt cctaaatcta
     1141 gctctttctt gtgggtcaat acctgctgtt ggttcatcaa agactattat cttagggtcg
     1201 tttagtaaaa cttgtgctat cccaactctc ctcttcatac ctcctgaaaa ttttcctata
     1261 gacttatttc tcacatcgta aagacctact aaatctaata attctcctat cttactattt
     1321 aaatattttc cccttaaacc ttttaaagct ccaatatact gtaaaaattc ttttgctgta
     1381 aaatttttat aaactccaaa ttcttgtggc aaatatccta aaatatttct ataattagca
     1441 tccattttaa atatatcctc accattataa agtatctgac cttcatcagg ttttatcaac
     1501 gttgttattt gtttcattaa tgtagatttt cctgctccat ttggtcctag aagaccataa
     1561 actccatttt ccagagttac tgtaatcttg tcgtttgcta cttttttacc atatatcttt
     1621 gaaagatttt ctatttctaa tttattcatc ttaactcccc ttttatctac ttatataatc
     1681 tgattttatt actaaaaaac ataatgacat agcacctatc aatattgcca tcaaaagtcc
     1741 tattgctata ttgacaatgt ctatattcac tttaaatatc ataatatcac ctagataatt
     1801 tcctattatt ttattaaaac caaatatcaa atcataataa tttacatttg atacaactga
     1861 aagtaatttt ttaggtaata tatggattac cgacattatc ttagttatag atataataaa
     1921 tataaaaaaa cttattgaag atattgaaga tttacttata aaagacccaa atatggctat
     1981 catggcccat ccttcaaaca tcaaaagctc tatgcctata tttaatatta catactttgt
     2041 tatacttaaa ttagttttca atgcaactac attgtctact attctaaaag attctaagtc
     2101 tccattaaca ggtactcctt gttgcataca agttataata aatattccca ttaaataaac
     2161 agaataaatt actaatggaa ctaataaagc aacaaatatt ttagacttta atgctttttt
     2221 cttctctttt gatgatagta tgatatcttt taccgaagta acgacttcat ctacgtaaat
     2281 attggatata attatagata taatgattaa cattcctaca tttataagtg aatttgtaac
     2341 tctatagtta aatactttcc aaaaactaat atccttatat tcattcacac ttagattatc
     2401 aagttttttc tttgacatta attcaatttt tttagaaaat tcacctgtat ctgtttgatt
     2461 tgaaaactgc tttaaaacat taatcttatt ttgaaatttc tcattaccta tgtcgatacc
     2521 acttctcgta tcttctatac atcctttttc tttatctata taataacgtt ctgtttctaa
     2581 aacaggcgta ataaagatac taatcattag tattaacact aatatcccta gagatataaa
     2641 catactctta gatttagcca atttttttat ttcccaattt accaatatat ttctcctcct
     2701 aaatcatttt aattacacat atatattaaa acttaaatct tatataaata taatactaac
     2761 cttaa
//
LOCUS       DAEPOG010000279         2758 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEPOG010000279 DAEPOG010000000
VERSION     DAEPOG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.282"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..153)
                     /locus_tag="KR432_003584"
     CDS             complement(<1..153)
                     /locus_tag="KR432_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0642088.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="KR432_003585"
     CDS             complement(327..1193)
                     /locus_tag="KR432_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0642089.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1419..2288)
                     /locus_tag="KR432_003586"
     CDS             complement(1419..2288)
                     /locus_tag="KR432_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0642090.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
BASE COUNT          912 a          476 c          287 g         1083 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caatcaatac aataaatata aaaattgtat
     1321 tatacaataa aaaagttcaa taacatttta ttataaaata ttattgaact ttttataaaa
     1381 attaatttaa gtgtagttaa attaagtatt aaactatctt atatattaca atatcctact
     1441 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
     1501 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
     1561 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
     1621 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
     1681 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
     1741 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
     1801 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
     1861 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
     1921 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
     1981 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
     2041 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
     2101 gctactttat ctaaattatt aaaaacttca cttttattca taattcttac atcaacatct
     2161 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
     2221 tctttaatat ctatgttctt atattctcta acagactttc tatttaatat gattgtttta
     2281 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
     2341 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
     2401 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
     2461 taaaaacata ttataaaagt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
     2521 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
     2581 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
     2641 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
     2701 agttcttcct atatttatat aatacatttt atatatgaat tttatatgag attggtat
//
LOCUS       DAEPOG010000280         2754 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEPOG010000280 DAEPOG010000000
VERSION     DAEPOG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.283"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..803
                     /locus_tag="KR432_003587"
     CDS             <1..803
                     /locus_tag="KR432_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBH0642091.1"
                     /translation="TIMAVLAIKHDYKYNNLRKDASINLNSNKEAVTVMSNQSLPKGN
                     TNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSNKIVERSTNDKIKNDIFEFYNK
                     SYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSIKVRYYTYSGGAHGFYQDIAYN
                     VDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELEKKDEENIGIYNFKGIKENQNF
                     YLQDENLVVYFDLYDITPYAAGIPEFSINKALISSMLKPEYVDLFDLK"
     gene            870..1859
                     /locus_tag="KR432_003588"
     CDS             870..1859
                     /locus_tag="KR432_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBH0642092.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            1951..2562
                     /locus_tag="KR432_003589"
     CDS             1951..2562
                     /locus_tag="KR432_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0642093.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         1141 a          270 c          503 g          840 t
ORIGIN      
        1 tgactataat ggctgttttg gcaattaaac atgactataa atataataat ttaaggaaag
       61 atgcttcaat taatttaaat agtaataagg aagcagtgac tgttatgagt aaccagtcat
      121 taccaaaggg taatacaaat agtaaacaaa ctttatcagt aagtacaata ggaagtaaaa
      181 caatagtgaa aaatgatgag tatttagaaa gtactataaa catgcctgta ataacaaatt
      241 caaataaaat tgttgaaagg tctacaaatg ataagataaa aaatgacata tttgaatttt
      301 ataataaatc atataaagaa gcaaagcagt atcttaagga taatcctgac gagaaaaata
      361 agtttgttgc aaatgtagac tttgaactta aaaagaatac tgattctgct cttagtataa
      421 aagtgagata ttatacttat agtggtggag cacatggatt ttatcaagat atagcatata
      481 atgtggatat gagaactgga aaatttttag aattaatgga cttatttaag gataatacta
      541 aatataaaga agttatagat gaagaaatta aaagacaaat agctgaactt gaaaaaaagg
      601 atgaagaaaa tatcggaata tataatttta aaggtataaa agagaatcaa aacttttact
      661 tgcaagatga gaatctagtt gtatattttg atttatatga tataactcca tatgcagcgg
      721 gaataccaga attttcaata aataaagcgt tgataagttc tatgctaaaa ccagagtatg
      781 tagatttatt tgatttgaaa tagaagatta aattaaatgt tatgctataa tatttagcat
      841 agaaacttat taaaattggg ggtataaaaa tgctactttt aaaaaaagac gacataaaaa
      901 aagtatttac aatgagagat gcaattgaag ctgataagga agcttttaga atttattgtg
      961 aaggaaagag tgtaaatcct ttgagaacca atatatctgt tccaagtcaa gatgctagta
     1021 tgttatttat gccaggatac gtagaagaat taggctgtgg gggaataaaa attgtatctg
     1081 tatttcctaa aaatgctcaa aaaggcaaac cagtaattcc agcaacagta ctattattag
     1141 atggagaaac tggagaagtt agtgcagtat tggatggtac ttatgtaact cagattcgta
     1201 ctggagcagc ctctggagca gctatagatg tattggctaa aaaagattct aagattggag
     1261 ctttgatagg tctaggagga caaggtgagc cacaacttga agccatgata gaagctagaa
     1321 atttggatga ggtaagagtt tttagtagaa acagggaaag tagagaaaaa tttgcagaag
     1381 aaatgaactt aaaattaagt aaatataata ctaaaatagt tgcagttgaa tcatctgatg
     1441 aagccataga taacgcagat gtgatagttt tagctactcc atcaaaacaa ccagtactta
     1501 atggaaactt agtgaaaaaa ggagctttaa taagtgcagt aggttcatat atgccagata
     1561 tgcaagagtt gtgcccaaac tgtttaacta gagcgtctaa gatatttttt gaatcaacag
     1621 atgcagtttt atctgagtca ggagatatat taattccatt aaaagaaggt aagataagta
     1681 aagatgattt tagtggtgat ttaggaaacg ttataaatgg aactattcct ggaagagaag
     1741 atgatgatga gataattgta tttaaaactg ttgggatagg tgtacaagat gtcgtaacag
     1801 caaaaagaat atacgataaa gcaaaagaaa acggtattgg aatagactgg aagtggtaaa
     1861 atattcaaaa taattctata gttagacaaa tttatgttat aatgttgata aataaatttt
     1921 ttattatttt aattgaggga ggatgtttgt atgaaaatgg taaaaaagtt aatattgtct
     1981 acgattatgg ctctagtttt agttctaact atagggtgtt ctaatgcaga agaaaaagta
     2041 aagtcgaatt ttgaagctaa tttagctgca ttagaaatgt ataagtatga tgaaaaagga
     2101 atttctgatg cattaacaga agctgaatat aaacagattc aaacttataa cagcaaggac
     2161 aaggtttcaa tgattgctca attgacacct ctttatggta aagaaaatgc tgaaaagata
     2221 tacgaaacaa ttgttaaatt aattcaaggt gttgaaaaga cagtaacagt ggaatcttct
     2281 agtaaggata agataacttt gaaagttgta agtaaagctt tagatactag taaagtaatg
     2341 agtgaagcga acactttagt aaaatcttat gtaactgatg aaaagaatta cgataaaaaa
     2401 gaaaaagaag ttcaagatga tatgacaaaa atgattatag acctttacaa taagaaacca
     2461 actaaggaag ttacaactat gattacatat actaaaaaag atggtaaatg gcaaccacct
     2521 agtaatatac aaaagttatg tgaaggaata tatacaattt aatttatttt aaagacacaa
     2581 aactatcttt taagcatata ataaaatgtt tggaagatag tttattttat tttcaaagta
     2641 taaattatat tttataaatg atttatttta taaataattt atcttgataa atagtaaata
     2701 atttacttta ctaataataa ataatttatc ttaccaatta atttactgta ttaa
//
LOCUS       DAEPOG010000281         2741 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEPOG010000281 DAEPOG010000000
VERSION     DAEPOG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.284"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..490)
                     /locus_tag="KR432_003590"
     CDS             complement(<1..490)
                     /locus_tag="KR432_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBH0642094.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIP"
     gene            complement(590..1039)
                     /locus_tag="KR432_003591"
     CDS             complement(590..1039)
                     /locus_tag="KR432_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0642095.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            complement(1029..>2741)
                     /locus_tag="KR432_003592"
     CDS             complement(1029..>2741)
                     /locus_tag="KR432_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0642096.1"
                     /translation="DLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLLLNSNNIT
                     SINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIRKSIAKMM
                     DDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLGYTINQPY
                     YINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIENRYRVKIT
                     KDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNINLKNDDL
                     LQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEYDNLKNIS
                     IDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTIVDIVPMH
                     QLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKKSMNLDTN
                     LLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLNKGIMNIN
                     NVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILFKNNLLEK
                     TNGN"
BASE COUNT          867 a          449 c          248 g         1177 t
ORIGIN      
        1 taggaatctg tctatataat ataggaatta aattatacat tttacctttt attgcataac
       61 tatatccttc tattttgtat aggtcttcat tgtagatatc tatcaatata tcgaaaactg
      121 actttcttat ttttgctgac caatctctac tacatttttt tatactactg aataaattaa
      181 ttaagctttc tttttcctca tttaataaaa ttaaatcatt aaaaaaagat atatcaaatt
      241 gaaatactaa tctttcactt cccggtgatg gcagtatata atgaatatct cctcctttta
      301 ttatcactat ctcgcctttt tttaattgca taggcttgtc atttataaat agatttacta
      361 caccttctgt tataagtaat atttctattt ccttatgcca gtgtggtgtt gttaatactt
      421 ctccttcatt tgatagacta cgaaaaggaa attcatgtat taatttaggc atttctaaat
      481 atattgccat agatatcctc tcctctctag tgtctaaaaa ttaatctata ctatttaaaa
      541 gaatacacat tttattttaa aaacctcaaa tttataattt atacaatacc tacacttcat
      601 aaaatatatc taatatttct ttttttgtat gtacatttaa aagtctttca cttatatttt
      661 taccacctaa aatgtaagct attttagata ttaatatcat atcttcattg cctccagatt
      721 gtggcatagc aaacataatt attaccttta caggtaagcc atctatactt ggccaatcta
      781 cacctttttt agatttacct attactatgg atgccttctt tacatatttt gtttttccat
      841 gtggcatggc catttgataa cctatactag tagagcctat tttttctcta tgttttaatg
      901 cctttataaa cttacttttt aaagttatac ttcctgtatc gtataattta tctactaatt
      961 cctgtattat gccttctttt gtagttgatt tcaaatctaa aataatgctt tcttctgtta
     1021 atatttctct aatttccatt tgttttctcc aatagattat tcttaaataa tattttaata
     1081 tcctctttgc tcttgcaatc tattatatag tttatatagt tcttttgcct atataaattt
     1141 gatatttcca ttaaaatatt ctttaaaaaa ctttgattac taatttctaa ataaatatca
     1201 atcgttctag taacattatt gatattcatt atccctttat ttaatttact ttgcttatgt
     1261 atatatatat tcaaatcttt tcctaatgaa atagattcat atttactccc taaattcaca
     1321 cctattaaac tttccatctg agctacatca aaattaaaat atatattatc ttctaaatac
     1381 attcctaata agtttgtatc taagttcata ctttttttag gtttagaaag taaagcattt
     1441 tttatattat taatatcact ttccattaat agtactgata caactatatg taatttatct
     1501 ttcaaatcta agtttacagt agatattaca aagtctacat catctaaatt tcgcttactc
     1561 aactgatgca taggaactat atcaactata gtccattcag gaaattctct ttttaatctg
     1621 gctgcaagga gctgagatgt tccatatcct gtatgacaaa ctactattac tcttttacta
     1681 ctcatatttc tttctattgc cgcttgaaaa tatgtggcaa gatacgaaac ttcatctata
     1741 ctaatattct tcagattatc atactctgtc ataatattta tagtcatcat acataatccc
     1801 agcacatctg aaaatcgctc ttgtatctct cctaacaacg ggttttttat ttggatatca
     1861 tatcttaatc tgtttaacat aggccttaca tgagatgcta gacttttttg tagtaagtca
     1921 tcatttttga gatttatatt taaaaattgt gacatattct ctatcattat agatgaaact
     1981 ttttcacttt cacttaatat atctgattca tcattataac tactattatt ttcgctacta
     2041 aatccagatg ataccaaaaa aatatatatg tacataattt catctttcgt tatttttact
     2101 ctgtatctat tttctatttt atctataagt gtagtgacat ttttatatac taactcgtcc
     2161 aaattatcta cagaaatttc tctttcttcc tgagattcta tttgattacc ttcttcgatt
     2221 ctctttaaac atataagtat atgggtaatt aaatttatat agtagggctg atttatagta
     2281 tatcctaaat tgtattctaa ttctgtaatt atagtttcta caaatataat agaatcaata
     2341 tcaaataaat ttatcaatgc actaaatgta gcagaatcta atcttgttaa ctcagatata
     2401 tcatgttgtt cataattttc atctaataaa tcatccatca ttttggcaat cgatttcctt
     2461 atatccactt ctttacctat aatcttagtt ccttctaaag ttttattaag ttttaaattg
     2521 tattctttta tccatgcctc tatatattta aaatcattta ctatagaagt tttacttaca
     2581 taatatttat ctgctaactg atttattgaa gttatattat ttgaatttag taataaatac
     2641 tttaaaatct ccattcttct ctgctctact gataaagatt tattttcctt cgattgtata
     2701 tttaaactat taattaagtc tatgttcttc tttgctaaat c
//
LOCUS       DAEPOG010000282         2711 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEPOG010000282 DAEPOG010000000
VERSION     DAEPOG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.285"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(4..555)
                     /gene="mobB"
                     /locus_tag="KR432_003593"
     CDS             complement(4..555)
                     /gene="mobB"
                     /locus_tag="KR432_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH0642097.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(560..1741)
                     /locus_tag="KR432_003594"
     CDS             complement(560..1741)
                     /locus_tag="KR432_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH0642098.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(1952..2686)
                     /locus_tag="KR432_003595"
     CDS             complement(1952..2686)
                     /locus_tag="KR432_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642099.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          844 a          494 c          217 g         1156 t
ORIGIN      
        1 aaattattct tttaatatgt tttttatttc aacattgctt atacttaaaa tttcattttt
       61 tatacaagac tctatgtact ttgatatatt tttataatca tttaaattaa actgttcaat
      121 attcaattta tattctatat cactgacaat acctatcaaa tcagacttat tagataaaat
      181 atcctcatac ttaccttttc tagttacttc caatctttta aatctataat ttttatatcc
      241 ttctatgata atcaagtcca tatcttcata taattgaatc attttatata atggattctt
      301 ctcttttagt tcttcaatat atgctactcg tttatctgat attatacaga cttttctcgc
      361 cccagatttt ctatgtttat aagtatcttt tccttcctca tccatatcaa aatcatggca
      421 ggtatgttta ataactccaa cacgataccc ttttttattt aattctttta ttatgccttc
      481 aatcaacgta gttttaccta ctcctgaata tgagacaact gacaatatat tatacataaa
      541 attgtgtcct ttcataaaac tatctaataa caatctccac taattgattt tcttttattg
      601 tctcattctt tttaatctta atcatacaat tacttcttag agttgcagat aatctagctt
      661 ttgcatcttt cttatcattt agctctacat acaatttacc ttcacttgta tatagttttc
      721 cacttacaaa tctatcttta tttgaaagtt tattaaattc tcctttaaat atagctttta
      781 tatgatgtat tcccttctta tatgttaaat actcaataac aggaactatc acattatcaa
      841 ataccactat tgctgcatgt ggattaccag aaagaccaaa taatagcttt tctcccaata
      901 tacctacata acaagctcca ccaggtttaa tgtttatctt ccaaaataat tctgttgccc
      961 ctattttttt gaatacatct ggaattaaat ctttatctcc aacagaaaca ccacctgttg
     1021 taattaaaaa atctacactc tgtaacatat tctttacctt tttttctaac tccaatatat
     1081 catcagaaca attgcatatt gtatctgcct gtattccaag ttttaataat cttgaatata
     1141 aggtatatct attactatcg tatattttac cattctctaa agtctcattt atatcttgta
     1201 gttctgtacc tgtattcaaa attccaactt ttaaatcatc ataaacagtt actttggata
     1261 ttccaagact tgctaatact ccaataccat catatcctat attctgattt ttattaataa
     1321 tcaattgccc cttttttata tcctcaccct taaatatgta attatcgtat tcctttaatt
     1381 ttacatttaa aattatcata tcacctataa ccgttacatc ttcttttttt attacacagt
     1441 ttgcaccctg tggtatcttt ccaccagtca ttatcttcac tgcttcattt ttatttaata
     1501 ccttattaaa tacttctcct gcatacaaag tccctataac ctttaaattt ctattttcag
     1561 aatttgcatt atatgcataa ccatcatagg gtgacctatc aaatggagga ctatcaattg
     1621 ttgaatatat atcttctgca actactttat ttactgcatc tataagattt actatcttga
     1681 ccctgtttat tggtaaaaca tttttttcaa taatttttat ggcatcttct aattctatca
     1741 ttttaaatac tccccaatat attttttatt taagatattt ctatgtaata tattatagat
     1801 tataaaagtt ggaaatactt ttatcttata tttaattata actaatagtt atctataagt
     1861 gcagttcaaa ttttataaat ctaataaaaa aactgttccc agctagaaat ttccttctat
     1921 cttgaaacag catcatatgc tttagaatat tttattctaa taaagttcct tttattattt
     1981 ttacaatttt atcactttca atagttttta tagattttat tttgttatcc acaatactta
     2041 ttgtaagtac ctcttttttt aatattttgt tatcttcttt caatctataa ctcttaacta
     2101 ctttactacc ttcatgatta tatgctgtta aatcataatt cgcatctcct acaatttcat
     2161 ttattctaga ctcttcaaca ccaacatact ctcccaggtc aatacgtcta aaattttcta
     2221 gtgatacagc cgatttttca tggtcacttt tttcaatcac aacactagat tcagcatcaa
     2281 tgactgatat accttgtgat gagaaagaat tggtttcaac attgacaact ttattttcgt
     2341 taatatcaac atataaagca cttccatcta gttcatcatt tccagtatat tttggataaa
     2401 ctaatgtcat ataacttgat gtatttatat cttgcattgt attcgattct tttaaaactt
     2461 taggttttat ataataaaat gtattataag gtatacccat attctttata atgtacttat
     2521 aatctttatt cataatttca ttaacatcat tttgaacttt tgtcatagtt ttttgctgtg
     2581 ttgctttact tgctacttga tttttctctt tagtattttc tttatgacat ccaacaagta
     2641 taatacctaa aatagttaca caaactaatg acctttttaa cttcataaat caatcctcct
     2701 ttcttaaatt t
//
LOCUS       DAEPOG010000283         2606 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEPOG010000283 DAEPOG010000000
VERSION     DAEPOG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.286"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(194..1006)
                     /locus_tag="KR432_003596"
     CDS             complement(194..1006)
                     /locus_tag="KR432_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0642100.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(1376..2278)
                     /locus_tag="KR432_003597"
     CDS             complement(1376..2278)
                     /locus_tag="KR432_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0642101.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(2328..>2606)
                     /locus_tag="KR432_003598"
     CDS             complement(2328..>2606)
                     /locus_tag="KR432_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642102.1"
                     /translation="DEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENLYDD
                     WHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
BASE COUNT          918 a          394 c          224 g         1070 t
ORIGIN      
        1 tacttgttta taaaaatata ctatagataa atttcacata aaaataataa ttcaagtatt
       61 gatttttata taagaaaatt tgtaatgtac taaaaataac ataaaagttt ccatttttca
      121 atatgtaagc ttttcttatg ttattttatt gttaaaggtt tcaagttttt atttattcat
      181 tttaaaaaaa ttattatttt tgtaaaacta atcccatagt catactatcg tcagaatatg
      241 cttcaccctt aacattacta aaaaactcaa cagacttaaa tccaatattt tcaacttcta
      301 ataagaaact ttcttttgaa aaatagtgat tccaaacata aaatacattt tcatcttttt
      361 cggttattac aagagtttgt tctaatgtat tataatcatt gtatttacaa ttatcttgta
      421 gacacatata tttttcatta ctccaaaatc ctccatcttc atttatttcc caatatttaa
      481 tttcctcaaa attattgtat ttatttattg taaatacatc taatattaat tttcctccag
      541 gttttaaact atcataaatt ttttccatta ataaccttct attttcagtt gacaaagctc
      601 cataatcaca ataaattaaa gtagccaaat caaattcatc attatagttc atatttaaat
      661 aactttgaaa caagtaattt atgtttaaat tagtctcctt atttctattt tgtgcatagt
      721 taatagaacg ttttgagaaa tcaattccag ttaccttata acctttttgt gcaaatttct
      781 ctgcatataa acctggacca caacctaaat caagtaaatt aggataatta actggatttg
      841 caactgtaac tatccaattt acagaatcct cgataaagtt aaaatttcta cttgctcctt
      901 caaaattagt gtctaaatgt gcttttaata attgttttga aatatactca tcatcccaaa
      961 aattaatttc actttgttta taaagctctg gtctttctaa atacatacat aatttgttta
     1021 acaaaacata ccactcctta aaatttattt ttaaaataaa aaaaccacag agtttactct
     1081 gtggtttaac ttcaaaaaga tgagtttaaa taagagtaaa atagaaccaa atatttttaa
     1141 gcacaacaat aaaactatag ctttactttg tttgcttatt aaatttgatt ttattcactc
     1201 ttttccagca atacttatat caacgatttt atgatatcaa atatcaccga acaacatcat
     1261 cctttcatat ttttatttta tgaatcaatt atatatttaa attttatatt tgtcaataaa
     1321 ttttaatttt aataaatttt aatcatcata gtaaaaaaaa attttttata taaaactaat
     1381 caaaaaaaga tatctgtcca tctcctgagt tttttaactt ttccttcatt attttagagt
     1441 aactgcttga tagagaatat ttatatctaa gttcattaac cattttatac aattgatttt
     1501 tatactccct attaagtgaa cctgcactat atagtattga taatttatca aataattcag
     1561 gaaactcttt tttaacaaag tcaaaaaaaa cacctctagt tatacccctt aagtataaag
     1621 tcccaggtaa aacataatgt acattacatt cttttgcatg tctaaatagc gaatttatat
     1681 tatcaaaatc atctgtaata tatggtataa ttggcataat atgtagacca actgatgcgt
     1741 tagtttttct aaattctttt aacattttaa atcgtctcat tgaatccaca ccatttggct
     1801 caataagcct ttgtgtatct tcatttactg tggtaattgt agatgctaca ttgatatatg
     1861 taatcctaga tagcttatca attaaatcgt aatctcttaa aattaaatct gactttgtag
     1921 aaataatagc tggtgtcttg tattttatca gcaggtttaa tatttctggc ataattttat
     1981 agtcagcttc tattggttga taactatctg taactcctcc tatattgatt acttctttct
     2041 tccatttact gcttttaagt tgtctctcaa gtttttctac tatatttgtt tttacataaa
     2101 tctcttcaaa ataattattg gagttaatgt acttatgaga gtaaatagca taacaatatt
     2161 tacatccatg ctcacatcca cgataaatat ttaaatccca tttatatggc atactccttt
     2221 ttaatttatt acaagctatt tcacaattta ttggaatata ttttatttta ttatacaaat
     2281 aatcacctct ttatatacaa taatactata atgttaatct atagtaatta aaacatgata
     2341 tttccagacc acaattcata atctttacta tatttacaaa gtaatatatt ccattcattt
     2401 attggtattc ctccacatgt tgctttttct tctaaattat ataagcattg ctttaaatac
     2461 attttattat gccaatcatc atataaattt tctaaattta aactatgttc attagatata
     2521 tcatttgcaa aatttctatt tttccatttt aatatacttg tatatttaag ttcattaggt
     2581 ttataatttc tttttaacat ttcatc
//
LOCUS       DAEPOG010000284         2598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEPOG010000284 DAEPOG010000000
VERSION     DAEPOG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.287"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(868..1905)
                     /locus_tag="KR432_003599"
     CDS             complement(868..1905)
                     /locus_tag="KR432_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0642103.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2002..>2598)
                     /locus_tag="KR432_003600"
     CDS             complement(2002..>2598)
                     /locus_tag="KR432_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0642104.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          861 a          391 c          329 g         1017 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac tcctttgaaa atatttttat aattttaatt atttgctaat tttgtgtata
      121 aaaaaaccac atagtgtaca aaactatgtg gtaaaaacac gaataagcca catagtttaa
      181 tctatgtggc tcttatatag ttaaatgaaa actttataaa caatatagcc aacatagata
      241 caatcccaaa attttaatag tcttaagggg tctgtatcta tgttaaaacg taggtacaaa
      301 ccctaaaaat agtggctata ataataataa tattttctat tgtttaataa agtattcata
      361 attatctctc ccttttaata ttttaattag tcagaaaatt atatgtattc tcgacttgtt
      421 ataaattata aattatacaa taataaaagt caactgctaa atttaattaa aatactaaat
      481 aatgtatact cagaatactt ttaaattaat gtattgtttg caaaaaacgt tatcctaagt
      541 tataagtgaa tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt
      601 atatcaatca aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag
      661 ctcaagtagt gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt
      721 ttatggaaaa tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc
      781 aaagggttag ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata
      841 ttacctattt tttgaaatta ataagtttta ttttgcagat tttgcatttt catctgactt
      901 tatatacttt atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc
      961 gttaattatt atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc
     1021 ttctaatact tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc
     1081 acaacaagct aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt
     1141 gttttcatta gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg
     1201 gacatataaa acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga
     1261 agtgctgatt cctttttcaa ctacttcata attatttttc ttagcataat catgtagaga
     1321 atcaacctgg atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc
     1381 ttttggagtc aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt
     1441 accagaaaca tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc
     1501 tgtaacagca gtcattatta ttggaatatc tttagtagca ttataagctg cttgtgcaga
     1561 tggagtagat atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact
     1621 ttgtgtagta ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc
     1681 tttatagcct ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagacgg
     1741 atgttccact aattgactaa tacctatatt tttttgtttt ttgctatctg tttttttatt
     1801 ttcatttgaa gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag
     1861 tactcctgct ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct
     1921 taaaatttaa taattatatt tgttcatata ttcatatgat agattataaa tatttctgat
     1981 taaaactaag attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac
     2041 tgttaaattt tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat
     2101 tatcaaccta tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt
     2161 tatatttttt tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg
     2221 gtcaagtgct gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat
     2281 aattagtgat agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata
     2341 tttttttaag tccaaagaaa ttccttcaag taagtgttcc aaattattcg ttttatgacg
     2401 aagacatttt tttaagttca ataatttccc tttattaagt gctagagata aattttctct
     2461 aaccgtcata gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg
     2521 agttctttta tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga
     2581 agattctttt ataagccc
//
LOCUS       DAEPOG010000285         2586 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEPOG010000285 DAEPOG010000000
VERSION     DAEPOG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.288"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            190..1809
                     /locus_tag="KR432_003601"
     CDS             190..1809
                     /locus_tag="KR432_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0642105.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2183..2377
                     /locus_tag="KR432_003602"
     CDS             2183..2377
                     /locus_tag="KR432_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0642106.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          964 a          306 c          371 g          945 t
ORIGIN      
        1 atatatcata aattttctaa aattaacaac aacttaaaaa tttaacattc tcttaacgta
       61 tacttaacaa aacattaata tttctattga caaaatttgt tattctatat tatggaaaca
      121 ataaatttta gttaataaag tttataattc agataaaaaa actattaatt tcttaaggga
      181 ggaaatatcg tgaatatagt aattagcctt atcggtggac ttggactttt tctttacgga
      241 atgagtttaa tgggagaagg tttacaaaaa tcagcaggag acaaattaaa aaaaattatt
      301 gaactcttaa caagtaatgt tgttatgggt gtactggtag gtacagtcgt tacaggtatt
      361 atacaaagtt caagtgctac tactgttatg gttgtaggat ttgtaaatgc aggtataatg
      421 aatctaagtc aagctattgg tgtaataatg ggggcaaata taggtactac agttactgct
      481 caattagttt catttaatct tgaaggaatt gcaccaattg ctttaggtat aggtatttta
      541 ttctatttat ttacatcaaa tcaaaaaaca aagcatttat ctgagatact tataggtttt
      601 ggtatattat ttacaggaat ggagtttatg aaagatgcag tagctccact tgctgaatat
      661 aaagcattca ctgatgcact tttatatttt agtaaaaatc ctgtattagg tatattggca
      721 ggatttgcca taactggtat tgttcaaagt tcaagtgcat ctatgggtat gttaatagca
      781 ctagcttctc aaggaatact tcctctatca tctgctttac ctattcttta tggagataat
      841 ataggtactt gtgttacttc acttttatca agtgttggtg caagtagaaa tgctcgacgt
      901 gcagctgtaa tgcatttaag ttttaatgtc attggtacaa taatatttat gcttgtttta
      961 aataaaccta tatcagccat agttactcat tttgacccaa ctgatacagc tagacaaata
     1021 gcaaatgcac atactttatt taatcttaca aatgttataa tattattacc attttctaaa
     1081 tacatagtta aattagcaaa tagacttata cctattaaag aaactgaatc agaaatagta
     1141 aataatacta agtacttaga tgaaaggatg tttagcactc catctatagc acttggtaat
     1201 actgtacaag aagttgtaag aatgggtcat aaagctacaa actctttgga acattcaatt
     1261 gcaggatttt taaataagag caatgaagat attaataaag cttttgagtc agaaaaagtt
     1321 gtcaataagt tacagaaaga tatattgaac tatttgttaa aactttctaa agaacctctt
     1381 agagacgatg aacgctttag aacagatttg ctttttaata ctgtaaatga cattgaaaga
     1441 gtctctgacc atgcagaaaa tatagctgaa ttggccatga gtgttaagga aatgaacatt
     1501 agtttttctg atagtgcgat aagagaaata tacgaaatat acaataagac aattactaat
     1561 tttaaggatg ccttaatagt tttagatgtt aaagattttg agttagcaaa taaagtttta
     1621 gaagttgaaa atgaagtaaa ttatttagaa aaaactttta gaaattcaca tatgattaga
     1681 ctaaataatg gaaattgtac aattgatgct ggtgtacttt atcttgactt acttactaat
     1741 ctagaaagaa tttctgacca ctcaacaaat atagtaaaac aagtattaaa acttaaacaa
     1801 aaaatatagt gattattttt atatataatc ttaatcaatg agaagtaaga agctatagat
     1861 aatgcttata tcatttatgg cttcttattt tttatgtaat actcaaattt ttatagtatt
     1921 ctcttattat taaacagctt ctgcctagaa tattccatat atatatgact tctttcataa
     1981 aacttgaact gcccataact aaagtcacaa gtatacataa ctatttttta atcaattata
     2041 accaatattt attttattta tcttcatttt aattcttcat tcaataatgt aataagatta
     2101 ttttttattt ggatactatt atatcatcta aatatgttat tatatatttg gatattttca
     2161 tattaatagg agggattcta caatgataaa ttatatggta gaaattatat atagcgaaaa
     2221 aataagcgaa gtagaaaaca tgttgaaaat tgaaaatata caatatgaat ttacttctcg
     2281 tgatggcttt atattttttg gtttttatga gatgacagat gcatatagat ttgagacttt
     2341 atgttcagat gaaaaaattc gatttaaaag aatataataa ataaaaaaag tacatatttc
     2401 taaactattt gtttttcaaa tttaggttgt gttttaatgt agtgttgata tcataaacaa
     2461 ttttaataat aaaaaagtag gaatattata ttaatatgct cctacttttt actcatattt
     2521 attaatatta tttatcaaaa ctaccctaca aactggaatt tatcattctt tcataatttt
     2581 aaacta
//
LOCUS       DAEPOG010000286         2489 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEPOG010000286 DAEPOG010000000
VERSION     DAEPOG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.289"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(359..1396)
                     /locus_tag="KR432_003603"
     CDS             complement(359..1396)
                     /locus_tag="KR432_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0642107.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(1389..2075)
                     /locus_tag="KR432_003604"
     CDS             complement(1389..2075)
                     /locus_tag="KR432_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642108.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2087..2407)
                     /locus_tag="KR432_003605"
     CDS             complement(2087..2407)
                     /locus_tag="KR432_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0642109.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT          843 a          339 c          332 g          975 t
ORIGIN      
        1 attagagttg caaaagaaaa agattataaa ataattataa tgttgaaaaa aatggtttta
       61 aattagatga catattgaat aaatttaatg attttgactt aagtgaagat gagataaaag
      121 aagtatatga aaataattga tgataaataa aaagtgaact tatataagtt tgctgaatct
      181 gagcctggaa atatttatgt gtatttttta atgaataaaa atttatttta aaaacaataa
      241 ttagaggtac agttaattat atttttatga tactgtactt ctagtttttt ctattgtaga
      301 tttattattt actaataaat ctacaataga agtgcttttg ttgtaagaat cattttatct
      361 aatgattgtt ttttataata gttaaatttc catttgtagt gcttaaatat aaatttgtaa
      421 taccattttt ataagtgtat aaatcactat gacgattcaa ctcaaagtca ctgtccaaaa
      481 caccagaaac cttatcaaaa tttaaagtga aacctggatt atcaggaata gacaaatcga
      541 cattggcaga agtagtttca cagtacagag aagacaacat tttgctagaa ttgattttta
      601 tattactgct agttgactca gctttaatgt cagaaaaaac tccagataag ttcatttttc
      661 cagaaactga actacaagta agatttttat tgtatatatc ttttaaattt atattgccat
      721 tagttgtttc taagtttaaa acatcaattt tcccagaaag ctgtatttca ccagatgttg
      781 atttgagtgt agcattttta ctatttagtt ttgttatatc aatgtgacca ctagttgtgt
      841 ctaaatttat tttattaata ttaccagaga gacctattgc acctgaaatt gtgttagctt
      901 caactgtatt acttcttatg taagtgctat atatatctgc tgaagtagta tataaggtta
      961 ttttatcata aagcttattg ggcaaatata gttcaagtat tgttggtttt ggaagattaa
     1021 ttccaatgaa gaatttttta gttgaattat catatataga aagagaattt ccgtcaacca
     1081 ttatttttgc tagttttttc tttgagaaat tactagggat tttttgaatt aatttgattt
     1141 tattagaatc acttctctta attctaactt cacctgaaat ccaatcaaca ttaatgtcat
     1201 ttattttatc tgaaaaactt tcatttttag ccactatata gttatcataa ttgtatttag
     1261 gattaagagt attaaaaggg aaattaaaag aatgtgaaat tatagttcct ataaggagtg
     1321 agataaatac aatagaaagt gatattgcca ggatactcca gatgaaaatc tttgccttta
     1381 taagcttttt attcatattt atcctcctcc ttgtagcctt ttagttgtat aattcctttg
     1441 acaatgttga caccagcagc acctattata aatagtatcc acgaaaagga ccaaatatta
     1501 aatagtatgc ttgtgattag atatatagaa actactataa tccaatatgc attcataaat
     1561 gatttataaa ctggatttat atgattataa ttttcctcaa aatcctcaaa ttctttgaat
     1621 ttatttgata aattatcatt gtatagagca tagtttgtcc ttgtcatttg attatagact
     1681 aataaaccta ctccaagtgc tattaatatg agcattaaaa gaatagctat agttggttct
     1741 atgccaaaac ttacaactga gactacaaat aggaaagggc ttattataaa aagagcaact
     1801 gctattgatg ctcttaaggc tcttttcttt ttttcttcaa ttaattcaga aggtagagaa
     1861 tatggttcct taacactttc aattaactca gataaatcac caattcctga gattactgag
     1921 ttatatgctt cttgttctgt tttaccatct tgtatttggt cgttatactt ttcttttaga
     1981 ttttcaaata attcatcttt aagttctttg actttttttg tctcattaac tcctgtaaag
     2041 ctactgtcaa tataagtacg tattttgtct aacatatttt cacctcctaa attaatatat
     2101 ctatcaatgc ttttgtatta ttccattctt cacggctttt tatatatttc tctttaccta
     2161 tgtttgttat tgaataatac tttcttctag ctccagtaac ttcatcaccc caataagagg
     2221 ttattgcacc agattgttca agtctacgaa atgctgtata caaagtagct tctttcaact
     2281 catacaatcc tttggttttt tcaagtatag atttattaat ctcataacca taactgtctt
     2341 tttcaagtaa atgttttaag ataattgttt ctgtatgacc tcttatcatg tcagatgcga
     2401 ttatcatgaa aatcacctcc aattaaatat ataatacttt gatatacttc gcctgtcaag
     2461 gtatatcatg agaaaagaca ttttattgt
//
LOCUS       DAEPOG010000287         2449 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEPOG010000287 DAEPOG010000000
VERSION     DAEPOG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.291"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            33..767
                     /locus_tag="KR432_003606"
     CDS             33..767
                     /locus_tag="KR432_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0642110.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(909..1400)
                     /locus_tag="KR432_003607"
     CDS             complement(909..1400)
                     /locus_tag="KR432_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0642111.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1749..2132
                     /locus_tag="KR432_003608"
     CDS             1749..2132
                     /locus_tag="KR432_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642112.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
BASE COUNT          956 a          276 c          355 g          862 t
ORIGIN      
        1 taagtgaata tatttaaaat ggaggaaact ttatgaaaca aaataaatat gatgatgata
       61 gattttttaa taaatacagt aaaatggaac gttctacaaa tggattagca ggtgctggag
      121 aatggcatgt tttaaaaaaa atgttacctg atttcaaagg aaaaagagtt ttagatttag
      181 gatgtggatt tggatggcat tgtcaatatg cagttgagaa tggagcaatt tctgctgttg
      241 gagttgatat ttcagaaaaa atgttaaaag aagctagaaa caaaacgcaa cttgataata
      301 taaattacat atgtatgcct attgaagata ttaattttcc aaaagatagt tttgatgtag
      361 ttattagttc cttggcattt cattatattc aatcatttga agatatatgt aagaatataa
      421 gtaattgtct ttcaaataaa ggtaattttg tattttctgt tgaacatcct atatttactg
      481 catatggaag tcaagagtgg tactgtgata aagatggcaa taatctacac tggccagtag
      541 atagatattt tgaagaagga gtacgtaagt caaattttct aggagaagag gttataaaat
      601 atcataaaac attaacaact tatttaaata cgttaatgaa aacaggattt gaaatattag
      661 aaattattga acctcaacca gaagaaaaac tgttagatac aattcctgat atgaaagatg
      721 agctacgtag acctatgatg ttacttgtat ctgcaaaaaa aagataagta aatataaata
      781 atcattattg tagaaaaaaa ttgtagaaaa taattacact aatggagatg attataccag
      841 tggtattaat tacaccagta gtaaggaggc aaaagcattc gttgcttttg ccatttttta
      901 agttgcaatt atacttctac gtagtatatt tcgtttccat tttcttctat aagctttttc
      961 atatcttcaa attttaaaaa tacagtagca gtattatcat ttggatgtat acctatttta
     1021 tccattttta ataaatcctt atcaaataca acttttacaa cagcctcttt atcatttaat
     1081 ataccaaaag gagtcacagc tcctttagta agtttcaagt atttttgaag tctttcttct
     1141 gatgcaaaac taagaggttt actatcaatt tgttgacgaa tatcttttaa atctgctttt
     1201 ttgtctttac ctaatacaac taaaaaatgt tcttttcctt tagcatcacg aagaaatagg
     1261 ttttttacaa caaattccat atgtaatgtt tttaattctt ccatttcttc aattgtataa
     1321 actggtggat gatttacagc ctcataaggt atgcttaact catctaattt tttatatact
     1381 tcctgttgtt gattgttcat gatttcctcc cattagaaat ttattttata tataaataat
     1441 attatcacag aaaactttaa aagaaaacgt tttatataat atagacaata gattatattt
     1501 ttataaagaa gcaagaatac tcaataaaac ttaaagaaat taaacaattt ctaaaaatac
     1561 cttgaaaata gaaccttatt tgccatttat aaattatata aaaagggatt atataaagtc
     1621 atatatgaaa aaactatatt atactatctt ttctttttgg aataaactat actttaaata
     1681 tagatattta atttaaataa attttacagt attaatagta aattatctta gaaaggaatg
     1741 tgctttatat gtctacagac aaagttgttg gaatcataga tgaagaaacg gcagaacttg
     1801 caggaattga atatactggt aaaatttatg cttcttctgg tgttataaaa catataaaga
     1861 aaaaacatag atgtcaactg agtaaggata tttttaatga tataatagac actataaaag
     1921 tggtattaaa gtcacctgaa tatataggaa gtcatcctaa aaaacctgga aaaagtgtgg
     1981 agtttataaa aaaaattgat aattatattt tagtagctac agaattagat acaaaaaaag
     2041 gatatttata tgtatcttca atgtatgcta taaaagaagc aaaattagaa agtagagtaa
     2101 acaatggaag agtaaaacaa tatgatggtt aacataatat tattaatttt ccccaaaata
     2161 ttgaaaaata ttattatata agttataata ttaatataca cgtagatatt tattagttag
     2221 atttgaggtt ggaaaaggca cccaatacgc tactatagag tagttaatag agatgcagga
     2281 tacgccgccc tgccaaatct aattaaatta aaaagagagg attaattcct ctctttttgt
     2341 ttttttgaga tactaattta atctatatag aaagattgaa ggtcatttat actaaaagtt
     2401 ttcagttata tacattaaac taattaaata agatatttat aatctatat
//
LOCUS       DAEPOG010000288         2394 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEPOG010000288 DAEPOG010000000
VERSION     DAEPOG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.292"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(329..2014)
                     /locus_tag="KR432_003609"
     CDS             complement(329..2014)
                     /locus_tag="KR432_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642113.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYYTMS"
BASE COUNT          886 a          352 c          189 g          967 t
ORIGIN      
        1 tttttatatt ttaataattt agtttaacta tttttctatg ctatttatat agaatatttc
       61 taataactca taaattctaa tcttttaata tctatccctc aattaatgga tatgaattta
      121 tttaatttaa atacttgata tttttcatat ttatataaaa atattacatt ttaaatacaa
      181 gttaataaaa atggaagaat atatgttaat gtaaatatta agaataatac taaaaaatat
      241 tactggaatc tcaaattttt aataatattc cagtaatatt taaataatat taaattaaga
      301 aaaatatttt cttatactta cataataatt atgacattgt atagtataat tctctaaaat
      361 ataaattacc atctaatagt tttttaaagc taccactacc tattatactc ccctctttaa
      421 gaacaattat attttcaaca ttcttaattg agttaaatct gtgagcaatc ataatagttg
      481 ttttatcttt caaaaattcc ataacattta gcataacttt ttcctctgta atattgtcta
      541 aagctgatgt agcttcatct aatataatta tcttagcatc tgaaaaccaa agccttgcta
      601 atgctagcct ctgcttttct ccacctgata atgtagttcc tttttcacct aatttagtat
      661 ctaaaccagc ttctaatttt aagtataaat tttttaaacc tactttttct aagacttcaa
      721 taatatcatt actttttata gtttttccaa atacaatatt ctcacgtaaa gttccatcaa
      781 atataggtga atcctgtgac acatatgtaa cataattata gtaactattt agattcatct
      841 caagtagatt ataatcatct ataaatatag ttccttcgct gggctttaat aatccaatta
      901 atagtttaat taaagttgat ttaccagaac cactctctcc aacaatagct actttttctc
      961 cacttttaat acttaaacta agtttattaa atatctctcg cttattatta tacaagaagc
     1021 taatctcatt aaattcaata tttccataca ttaaattaac ttctttacct gctaacagtt
     1081 gaccatcatt attaagttcc aaaatatctg tatagcgttt atatgctgct ttatctagtt
     1141 taaattgaat atacaataca ttaaaaatag ctataggagt atatgcatta tctagtaaag
     1201 ttatcaatgc aattatagaa cctatactta ttgttttagt tttccaacta taaactatta
     1261 ttaacatttt tatgaacgtc actattaatg aaaaaatcgt gaagaaagct tcatgaatca
     1321 gtgtcatttt aaccctagaa tcaataattt cttttttcgc aaattttgac tgacaaattt
     1381 catactcaaa tcttttgttt acccgaaata caaccatttc tacaaatcca cgaactaaaa
     1441 aatgattcat cttctcttca ttaattaata tatgctcttt gatttgatat agagctttta
     1501 ttagtaaatt agtaataata aaaactataa aatatccaat taatatggta aacataactt
     1561 ttttatcaat tctataaata aaaaccatac taaataaaat tgatggaatt atttttctaa
     1621 caacggctaa taaaaaatta aaaaatatat ttctgccagc attagctcca ttttcaattt
     1681 tttgaattaa ttttccagtg cctaacatct gatattctaa gtaatctatt ttactcattt
     1741 tttctaaagc ctttaatttc aaatctaaaa aaaaactatg ttctagtctt ctacctggat
     1801 attcatcaat atatcttaaa agacataaag taactaatac agttccatag ataaaaatta
     1861 tgtgaaaatt tatagtactg ttattaaatc tatctattag gctctgataa taattagcac
     1921 taaaactatt taaaagttct attcctatac ctagtgctaa atatataaaa actaataacc
     1981 tctctttttt tgctatttct attaaatatt tcataactgt acacctatcc ttatttattt
     2041 tactttatta acttaaaata attttataga taaaatgttt ctttatttgt ttaaatttag
     2101 tgtgattatt aatcatttca agttaacaaa acctaaagtt tttagcagta cagttctatt
     2161 tgttagcaaa ttattatttt actcagtaca ttatgctaac atataatcta actgtactga
     2221 gtaaatatct cgtttcatca tataaaatct cctttttata gtttaatata tatagttaaa
     2281 tttactatat aatctgaata tttatttttc tatcaccttt accagaaata aaatcaatta
     2341 tttattgtat aagcaaaact tcataatatt ttcaacatat aattattata caga
//
LOCUS       DAEPOG010000289         2359 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEPOG010000289 DAEPOG010000000
VERSION     DAEPOG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.293"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(308..820)
                     /locus_tag="KR432_003610"
     CDS             complement(308..820)
                     /locus_tag="KR432_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642114.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            1373..2254
                     /locus_tag="KR432_003611"
     CDS             1373..2254
                     /locus_tag="KR432_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBH0642115.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
BASE COUNT          899 a          326 c          220 g          914 t
ORIGIN      
        1 tttttgacgc aatcgttttt attcagtcat tttatattct tgatacaatc attcttacat
       61 tctattgttg tttgttttta acacaatcgt tttttgttga atcattttat attcttgata
      121 caatcattct tacattctat tattgtttgt ttttgacgca actatttttt attcagtcat
      181 ttaatatttt cgacacaatc attcttacat tctattattg tttgtttttg atacaactgt
      241 ttttattcag tcatttaata ttctcaacac aattaccttt tcattcaatc aaagtaatct
      301 ttaagcatta tttttcaagc tcatcaaagt aattatatac gataacctca cctgtctctt
      361 tatctgtagt ctttataatg cccttagctc catttacctc ttcttttgta aattcatcat
      421 ctaacacaac tttgaattta tcttcctttc cataaccttg ttgaaattca tctctcaaaa
      481 ttgcattttt ttctgctttt gccttcaact caaattcaat cccagatttc atttcatcaa
      541 aataatttaa tactacaacc tcattattca ttttattagt tgtctttata atccctttag
      601 ctccatttac ttcttctttt gtaaattcat catccaatgt tatattaaat ttgtcttctt
      661 ttccataatc ctcttgaaaa tccctcttca tttcattgta tttagtttca aactctttac
      721 tcttgtttaa ttccatagct gatacatttg tcaatgatgc tccaattgcc attactgtta
      781 atgccattac tataccactt ttaaattttt ttactttcat accttacctc ctaaattaaa
      841 tgttctcaat gaaattatat aaagaaaatc ctaagtttta cagtttcatt cttgtaacta
      901 aaattactat tatgtaacca aactttttgt ttcaactatt ccttaaaagt tgctttttta
      961 aggaatagtt gaagttaaat aataagattt tatctatatt gatagattta aaatcaaatt
     1021 tatccatata aataaatact tatttttgca aaataaaaat ttttaattac tccaatcctt
     1081 cttatttact taaaatcctg tgataaaaaa tttaaaagtc tttacattct ttaacaataa
     1141 tttatttact tttaacatac aaaatatata gagtatatca aaattatttt actgaatatt
     1201 ctaaaaataa gtacccccta ttagtatata tatttatata ttaaaaaaaa gttttaaata
     1261 aagttatatt ttttacaaca gttttgtaat taaaaacata ttgaaggtaa atttaaaggt
     1321 actatatact ataaatatgt cgacacaata aatttaaaaa ggagaactaa atatgaaaaa
     1381 aagattaata ataatgatat tgtctgttgt cttagtacta tcaagtatac ttacgatatt
     1441 tgcatattca aatattaaat ataataataa taaattgata tattctaata tgatagataa
     1501 aaaaactcaa aatagtgtaa aggaaatcct aaaagaaaat aaaattaatg aaaaagacat
     1561 agatacattt attaaagcag tcaacaatta taataaattg caagttaaaa tacttcaaaa
     1621 taatataaat atatctaaat ctggatattc ttcaatcaat gcaaaacaag ttccatataa
     1681 cctagaaaaa ttgcaagata attgggtgaa aaaattccct gattatatgg atgtaaattg
     1741 tagaataact gcttttagac tttttaaaga ttttataaat tcaaataaga aatttactgg
     1801 agactctatt gatttgaatg tcgacttaga cactataatg aataataaag atgctaaatt
     1861 tagcaccaaa gatgtagaaa aatttatcaa cttcttttct gcaataccag ctaaagatac
     1921 agatgataca atcaaaattg cagaacaaat aaaaaatgaa tggaaaaaaa gaaaaatatc
     1981 ttttaaaaat aataaaaata tatctattat aaatggattt cttcgttatc cagaaactaa
     2041 aaacgtattt ataggtcata caggcatatg tattaaaact aaaaatggca ttctattttt
     2101 agaaaaatat ggtgttacat caccttatca agttactaaa tttaaaaata aaaaaggtgt
     2161 aaaaaattat atgtttaata gattgaagat gtcagaaggt gaaattgaat tgcctgaccc
     2221 tataataatg gaaaacgata aacttatgaa ataataactt taaacgcaaa atagtattta
     2281 taattactaa gtcaatataa tagagaaggt ctatagattt gtgtaaatac aatcttatct
     2341 acaatcttat ataagaatg
//
LOCUS       DAEPOG010000290         2331 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEPOG010000290 DAEPOG010000000
VERSION     DAEPOG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.294"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            127..891
                     /locus_tag="KR432_003612"
     CDS             127..891
                     /locus_tag="KR432_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBH0642116.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    1114..1327
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1581..>2331
                     /locus_tag="KR432_003613"
     CDS             1581..>2331
                     /locus_tag="KR432_003613"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH0642117.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTK"
BASE COUNT          936 a          231 c          394 g          770 t
ORIGIN      
        1 aatctaaaaa taatattatg taaaataaaa atatatatga catgatgttg ttgtattcaa
       61 aatattaaga taaaaatata tagaatatat ttaaaaatta ttatgtaatt agaaaaggag
      121 ataagtatgc agattataga tataaatata aataatattg acagtagaca tatatgctgt
      181 tcaatttctg aaaaaaaagg agaaaattgt gttttttcta aaaaagcttg gatgaaaaat
      241 aattttgata aaggtctagc ctttaaacga tttgatgtaa gaggtaaagc ttttatagaa
      301 tatattccag cagaaaatgc atggtgtcct attactgcgg acaattatat atttattaac
      361 tgtttttgga tatctggaaa atttaagggt caaggatatt ctaatctatt gctacaagag
      421 tgtgttgatg atgcaaaatc aaaaggtaaa tatggattag ttgtgctttc gtctagtaaa
      481 aaaatgcctt ttttatctga tgctaaatat ctaaaatata agggcttttg tttagcagat
      541 acatcagaac caaattatga gctattatat tttcccttta ctgataatgc tcctattcca
      601 tgttttaaga attgtgtaaa aaaaagaaaa ttaaatgaga acggtatagt tttatattac
      661 agtaatcagt gtccacatac agaaaaatat gctcctattt tgaaaaaaat tgcaaattct
      721 agaggtattg atgtaaatct tataaaattc gaaacagcaa aagaagcaca aaatgctcct
      781 gcaccattta ctacatacag tctttttttt aatggtgaat ttgtaaccaa cgagatactt
      841 tctgagaaaa agattgagaa atttttagat accaacatta aaaaaattta actaaaaata
      901 gatgtaaata ttgtattttg agattacatt attttataat ccaatatatt ttggaaattt
      961 cttaagttga tgaatgataa tataaagatt ataactcaat ttagatacaa aatgctggaa
     1021 attaaccata tagaaaatat ttccaaaaat ttaaaaatta tattgacaaa gttaaaaaaa
     1081 taaactatca tataaaacaa taagaaaata aattgaaatc ctgtgaaaaa gagagtagca
     1141 taaagctagt tgatagagag ctgggaatgg tggaagccta gtaacgaatt ttattgtgaa
     1201 tagagctttg gaggagatta ccagaagtaa tagtatggta attggtgaat ctcgccttaa
     1261 gagattagag tgggtaagat tatcttatca attagagtgg taacgcgggt gtatattcgt
     1321 ctcttggtct ttgaccaaga ggctttttta attttaaatg tacattttgt agatactttt
     1381 taatttgagt tttaatgatt attgtaattt taccaatgag agtggtaccg cggaaaatcg
     1441 cctcttagta tttataatgc taaggggttt tttgttttaa ttagaatatt ttaatctaca
     1501 tattatcaaa tgattatcat atagtaaagt ggaaagatag aattttaata ctatttgaat
     1561 ataaaaatgg agggaacaaa atgaataaaa taaaagttgg tattattggc gcaactggat
     1621 atgtcggggc agagttgatt agattattaa tgaatcatga taaagtcgaa gtgacagcaa
     1681 taggttcaaa ttcgtatgtt ggaaaaaata tagttgatat atatccaagt ataggatata
     1741 aaaataacat gatatgtata gaaaatgaaa aagttataga tatgtgtgat gttgtgttta
     1801 cagcccttcc acatggagtt agtgaaaagt ttgtaataaa agcaattaaa tcgaaaaaga
     1861 aagttataga tttaggtgct gactttagaa taaaagatga agaagtatat agtaaatggt
     1921 atggagtatc ttttatagat aaaatcttac ataaaaaagc tgtatatgga ttaagtgaaa
     1981 tatacaaaga agatataaaa gatgcagata taattgcaaa tcctggatgt tatccaacta
     2041 gtatttcttt accattgatg ccattactaa gttctaaatt gataaaaaat aataatataa
     2101 taattgattc taaatcaggt cttacaggtg ctggtagaga attatcgatt agtagtcact
     2161 ttacggaggt taatgaaaat atcactgcat ataaaatagg gaaacacaga catactccag
     2221 aaatagaaca aaatttaagt gaatcctgca aagaaaaagt aagtgtagtt tttacaccaa
     2281 acttaatacc agtaaatagg ggaatcttat caactatata ttgtacaaaa g
//
LOCUS       DAEPOG010000291         2323 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEPOG010000291 DAEPOG010000000
VERSION     DAEPOG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.295"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            87..485
                     /locus_tag="KR432_003614"
     CDS             87..485
                     /locus_tag="KR432_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642118.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    921..1184
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1335..1886
                     /locus_tag="KR432_003615"
     CDS             1335..1886
                     /locus_tag="KR432_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBH0642119.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          973 a          214 c          323 g          813 t
ORIGIN      
        1 tatataagaa acttgaaaaa atttgtaatt tttagttata tatatattat aatgaactag
       61 ttaaaaattt ttatgagggg gaaaatatga ataagaagaa aaaattgatt gggatagcta
      121 tatcttttct acttataatc agtattataa atccattaca agtagaagca gaagctatta
      181 ttgagtctag tgtatacatt tcttcaataa aagagaatta tacatatgat aatactaaat
      241 ttaaaactat taaatctaaa agtagacata aaagtaggaa atataataaa agtaaagtaa
      301 agttgaaaaa acgaagtata agaactaaaa agtatggata tcaaactata aataaatata
      361 aaagacgaaa tgcaagaaga cataatatcc taacaaaatt agtattgtcc ataatagtca
      421 tatttttggt tatagtttta gtagtgctaa tatttattaa aagaaagaag aaaaaatttt
      481 actaaaaata attaaaagaa tatcattttt ctaatcattg gtatctattt aataatatga
      541 tgtaattaaa atggagtaat attctgtttc aaaggaggca tataaataca ggaatgagta
      601 ttaaatgatg gttaattaag taaatttatt gataaatgta cttaattaac tttgaagaat
      661 ttaattcctc atgaatcaaa atataaaatt acatcaacag ttattcacaa aattaatata
      721 agaagaatta gagccaaaga gcagactatt agaccattaa agtaattatt catagaattt
      781 tgtatatgaa aagaaatgta tagtataatt tacttttttt ataaaaaata gattacaaaa
      841 tatatttttt ataaaaaggt attgacattt tttagaaaaa agaatatcat tattttaaca
      901 acaaaattca atacaataaa ttaaatccta tgacaaggaa gagtagttaa atttaggttc
      961 agtatagaga gctgaggatg gtggaatctt agcaagaaaa aatttgatga atggccctta
     1021 gaggaataaa ttgaaatcat agttttgaga gtagacttta tcgtcagtcg cacgttatag
     1081 cgaataaggt atgtaagtac ctgataatga gatgtatgaa aattgaattt tatattaatt
     1141 tttatataat ttaggtggca acgcggataa tctccgtcct attagtttag gacggagttt
     1201 tttattttac tgaaaattta aaagatagaa ggtggtgaga aagatgttat gaatttcaaa
     1261 agctaaagat gatgatatag taggttggca aaaaaataaa ttgaaaatta aaaaaatagg
     1321 aggaagtaca aaaaatgaaa acaaatacaa aaaaattaac tttaaatgca atacttttag
     1381 cgatgggatt attaatacat caaatcacac cagctatagg attaccaatg aagccagatg
     1441 taccactagc aatgttattt gtaatattgg ttttaaatag agatgattat aaaacttgtt
     1501 tgatagcggg tatagtaaca ggaatattta cagcacttac atcaagtttt ccgggaggtc
     1561 aaattcctaa tgtaatagat aaaactctta cagcaaatat agtattttta ttaatgaata
     1621 ttagttataa tatgcctttt ataaaaaaat tagctaagaa aacacaagat actgtagtag
     1681 ttgcaataat aatgccaata ggaactcttg taagtggaac tatcttttta ttagcagctc
     1741 aagctatagt tggtcttcca ggagcttcat ttacagcttt attcttagca gtagtattac
     1801 cagctgtttt aataaatcta gttgcaggaa tatttttatt caagattgtt agtctatcaa
     1861 taagaagagt tagttatcaa gcttaactac gaaataatat tttactttat aaagcattat
     1921 aataactaaa taacataaga ataaagtaga ttataactat tttattctta tgttgtttaa
     1981 agatttggta ttatatattt atttttaata aaatatttat atagtaccaa ttacacttaa
     2041 tttaacagat aaattatcat acctatataa tattcttata tgtatctcat ttgtagttat
     2101 taaatataag atatatttaa ttgacttttc aagataaatt tttatattaa tctcctctga
     2161 tttctcattt tttgaataca ctaaaatttc caattaaatc aattatatat ttatagagtt
     2221 ttaaaaatat agtaatgtat atattttttg tggaaaactt gcttaaattt agcaaaaaaa
     2281 atagataatt catagtataa aataaagttt agtaaaaaat ttt
//
LOCUS       DAEPOG010000292         2309 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEPOG010000292 DAEPOG010000000
VERSION     DAEPOG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.296"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(19..699)
                     /locus_tag="KR432_003616"
     CDS             complement(19..699)
                     /locus_tag="KR432_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBH0642120.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(728..2017)
                     /locus_tag="KR432_003617"
     CDS             complement(728..2017)
                     /locus_tag="KR432_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0642121.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT          749 a          412 c          216 g          932 t
ORIGIN      
        1 tttaattaat actttatatt atagattgtg acattcttta gagttcaaac tttttaggta
       61 ttctctaact tccagtaaat tttcaagtat cttatgtgat tgtgatttaa tttgctcatc
      121 tggctctttt aaatctggta catttataca tcttatccca ccattgtatg cagcttcaac
      181 tcccataggc gaatcctcta tgacaataca attttgggga ttaacattta tctttttagc
      241 tgcttttaag aatatctcag ggtttggttt agagtttaca acatcatctc cacaaactat
      301 agcatcaaag taatctttta aatttgcctt agctaatctc tttactgctc tttctctctt
      361 tgttgatgtt gcaacagcca ttttgtatcc attttccttt aaaaaagaga ttagttcatt
      421 gactcctaat ttgataggag ctcccttcct ctccataaat tcaatcatat ttttagtttt
      481 ttcgtcatat aaatctatta ttggtacaga actatcgtat atatctgtaa gaccttctat
      541 tattccttta cgatttcttc ccataactga tgtataaatt tccttagtca tagtatatcc
      601 atacttttca aacgtctcca tccaaaattc aagagaaatt ctctctgagt caaacaaaac
      661 tccatccatg tcaaaaataa ttccttctac tttttgcata caattcctcc aatatttttt
      721 gatataatta tattacactt tttatgatta attttacaac cctcaattta tccaattcta
      781 atattgtaaa attgtaattt tcaaaagtta cgctttggtt tactttaata tttgaaccaa
      841 gcatatagga tatccaccca cctatactgt caaatccatc atgctctata tcaagtccaa
      901 aatacttatt aatatagttg atagattcag ttccatcaac taagtaagaa cctgctccta
      961 ttttcttaat atgagtctct tctttgtcaa attcatcttg aatttctcca actatctctt
     1021 ctagtatatc ttctattgtt accacaccag atgtaccacc atattcatcc acaactatag
     1081 caagttgaag tttttccttt cttattctct caagagcttt atctatagtt aaattttcag
     1141 atatatatat aatatctctc aatatttctt ctagttcaat tttattttca tttatctttt
     1201 gattgtataa atctcttata tgtacaaaac ctaatatatc atctttattt tttctgcata
     1261 ctggatatct tgtaacacct tcctctttta aaatagctaa tatcttttct tcagaatcac
     1321 tttcatatat acataccata tcagttctag gaaccattat ctctctaatt ttcttttctt
     1381 caaattcaaa aatattatca actaatcttt gctctgattc atctataagt ccacttttat
     1441 aactttcttc tactaatagt cttatttcgt cacctgtatg gggttcatca acctcatcag
     1501 cttgtgaata tccaaatggt ttaagaaata aatcagtact taagttgaaa aaatatatta
     1561 ttggatatgt taacttataa aatcctacta ataaaaaaga tgtactcaac attatttttt
     1621 ctgtattata aagtgctaaa gctttaggaa ccaactctcc tattacaacc tccatcattg
     1681 ttataattaa aaaagaaatg gaaattgata tagtatatat aatactttca ctaagattaa
     1741 atacacttac tattggtaaa attaactctt taactgtaga ttctcccatc caacctaatg
     1801 caagtgaaca aagtgttata ccaagttgac atgcagaaag ataggaattt aagttgtctt
     1861 ttattatttt acatcttttt gcattattat taccttcagt tatcatggta tcaatcctag
     1921 aactacgaag ttttaccata gcaaattcac aagctacaaa aaaagcattt atcaaaacaa
     1981 gcattactat aagtgttact tttaataact cccgcataaa gtattggttc ttataatgta
     2041 agaaatctat aagaactcaa agttcccccc ttatatttat aaatttaatt tataatatat
     2101 attctatcat atttattaat atatcctact ttattacaaa taatttttat aaactaaata
     2161 acccctttaa tatttatgac tgtctatttg ataaatatta aaggggttat ttagttatca
     2221 ttttttattt ctaaaaatct tttctcaaat gatttagtta tctatttatc caatttttta
     2281 cataatttaa tgattaaaaa tattaattt
//
LOCUS       DAEPOG010000293         2263 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEPOG010000293 DAEPOG010000000
VERSION     DAEPOG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.297"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..596
                     /locus_tag="KR432_003618"
     CDS             <1..596
                     /locus_tag="KR432_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0642122.1"
                     /translation="TLKDYKLGEENKVEKYKYSNKEYTNLVKIKETDTIKYITENKED
                     TANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETKKDEDKDYNKAAIDIKKAI
                     EIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKNNEAYKIDASDAYNLRGVI
                     FMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            721..2238
                     /locus_tag="KR432_003619"
     CDS             721..2238
                     /locus_tag="KR432_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0642123.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
BASE COUNT         1000 a          259 c          362 g          642 t
ORIGIN      
        1 aaaccctaaa agattataaa cttggtgaag aaaataaagt agagaaatac aaatattcta
       61 ataaagagta cacaaatctt gttaaaataa aagaaactga tactataaag tatatcactg
      121 aaaataaaga agatactgca aattttgata gatattttat aatcgaagat aatgaatata
      181 aattattatg gcataaagac tttaaaagag ctcttagctc taattatgtc aaaatcgcta
      241 ggtcagaaac aaaaaaagat gaagataagg attataataa agctgctatt gatatcaaaa
      301 aagctataga aattgatgat aaaaatccac ttgcatatta tacacaatca tatatttata
      361 tagagttaca acgatatgat gaggctttaa agtcaattga taaatcctta gaatatctag
      421 ataaaaataa tgaagcttat aaaatagacg ctagtgatgc ttacaattta agaggtgtta
      481 tttttatgag taaatcagaa tttaataaag ctagagaatc ttacaataag gcgcttgaac
      541 ttaacccgga taatgaatat gcaaaaacta atctaaatga tattaaagga ttataaggca
      601 ttaatattat agaaaataag tatgttgaaa gttaaattaa cttttgacat gcttattttc
      661 tatattttaa actctattga tttactatat aatagtgcat aaggagtgtg attacttatt
      721 atgagtgttg ccatatactt aagaaaatcc cgtgctgatg aagaggcaga aaaacaaggc
      781 gaatttgaaa ctttaagtag acataaatca accctactaa aactagcaaa agaacaaaac
      841 ttagatgtaa ttgagataaa agaagaatta gtttctggtg aaagtataat acatagaccc
      901 aaaatgcttg aacttctaaa agaagttgaa gaaaataaat atgatgcagt cttagtaatg
      961 gacttagaca gacttggtcg tggagacatg aaagaccaag gtatcatatt agaaacattc
     1021 aaagaaagta aaaccaaaat catcacacct agaaaaacat acgacctaac agatgaattt
     1081 gatgaggagt attcagaatt tgaagcattt atggcaagaa aagaattaaa acttatatca
     1141 agaagaatgc aaagaggtag aataaaatca gttgaagaag ggaatttcat aggaacttct
     1201 gctccatttg gatatgatgc tgtaacaaca ggtagaaaag aaagaatact tgtaccaaat
     1261 aaggatgctg atgtagttag gactatcttt gacttatata ttaatgaaga tatgggttgt
     1321 agtaaaatat caaaatacct aaataactta ggcattaaaa ctgctacagg tgctaattgg
     1381 tacaattctg caattacaaa tataataaaa aataaggtat attgtggcta cattcagtgg
     1441 caaaaaaagg actataaaaa atctaaaaat ccaaataaaa taaaaactgt taagctaaga
     1501 cctaaagatg aatggattga agcaaaagga aagcatgaac ctttaataag tgaaataaca
     1561 tggaaaaaag ctcaaaatat acttaaaaaa aacggtcatg tcagctatgg taatcaaata
     1621 aaaaatccac ttgcaggaat agtgatttgc aaaaattgtg gtagaccatt agtatataga
     1681 ccatatgcag accacgacta tataatttgt taccatcctg gttgcaataa aagttctcga
     1741 tttgaattta ttgaagctgc cattttaaaa tctttggaag acacggtaaa aaaatatcaa
     1801 ttaaaagcat gtgatttaga cttagataaa aataataaag atagtaatat agaatttcaa
     1861 aaacgagtct taaagggctt agaaactgaa ttaaaagaat taggtaaaca aaaaaataag
     1921 ttatatgatt tattagaacg tggaatatat gatgaagata catttattga aaggtccaac
     1981 aatataagtt caagaactga agaaattaaa gattcaatta agacagtaaa aaatagattg
     2041 agtactgtta aaaaagacaa ttctaaaata atagaagata ttaaaactgt gttatcactt
     2101 tatcatgatt cagattccct tggaaaaaat aaactcctaa aatctgtaat tgataaagca
     2161 atttattata agtctaaaga acaaaaacta gattcttttg aattaatggt tcatttgaaa
     2221 ttacatgaag accagtaata ccaataacat ataaagagta gtg
//
LOCUS       DAEPOG010000294         2249 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEPOG010000294 DAEPOG010000000
VERSION     DAEPOG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.298"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            35..2047
                     /locus_tag="KR432_003620"
     CDS             35..2047
                     /locus_tag="KR432_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0642124.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1004 a          216 c          321 g          708 t
ORIGIN      
        1 tgttggaaac tatttattat ataaggagaa ataaatgaaa aaatcagtag ctctggtaaa
       61 tgatagtaga aaagatttaa tagacttttt agaaaataat ttgaaattag tttttggaga
      121 ttctataaat attaatagat acttcataaa tgaaataaat gataatgata ttataaatga
      181 tgatgtgata ttagtaatgt cagtagaaag attagacaaa attataaata atatactaga
      241 taagaaaaaa gtgattgtgg taagaagaac ttttagggaa gataaaattt ataatctact
      301 atctttacca cagggtacaa atgctctaat cgtaaatgat tctgatgaaa ctactctaga
      361 aactataagt ttattttata aaattggagt gactaatatt agaccaattc catatatgaa
      421 tgataataac tataaaaata taaaaatagc tataactcca ggagttccag aaaaagtacc
      481 tagctttatc tcagatatat ttgatttagg tcacagatat atagatattt ctacatttat
      541 agaaataata aatttattac aaattgattc aaaggaaata caatctaatc tagttaaata
      601 ctcagaagaa ataataagtt tagatactgg tataaaagat aaatataagg aattattttt
      661 aaaaatagaa gaattagata caatattgaa tttatcaaaa gatggtatat tatttacttc
      721 aaaagatggt gaaataaata catataatag taaagtaaaa gatattttag acataaatga
      781 ggatatatat ggaaaataca ttgatgatat atttgtagat agtttaaaag ttttattgag
      841 tgagaaagag atactagata aagttgtcgt attcaataag aaatatataa atgtaaataa
      901 aaaaaacata tacaataggg atgaaaaaat gggcacatat tatagtttac aagaaataac
      961 atacataaaa aaattagaac aaaatctcac taaaaaatta agagaaaaag gacaaatagc
     1021 aaaatataca tttaaggata taaaaacaaa tagtcctaaa atgtttgaat gtatagattt
     1081 agctaagaag gtttctaaat ctgacttaag catacttatc agaggtgaaa gtggaactgg
     1141 taaagaactc atagctcagt ctatacataa caactcaaat agaaaaaatc aaccttttat
     1201 agcagtaaat tgtgcagccg ttcctgaaaa tttattagaa agccagctat tcggatatga
     1261 caagggaact tttactggag gtctaaaaga tggaaaacaa ggattatttg aacttgctaa
     1321 taatggaaca atattcttag atgaaatagg cgatatgcct ttagaattac aaacaaaact
     1381 acttagagtt cttcaagaga agcagataat gcctgttggg tctcacaatg taattaatat
     1441 agatgttaga atcatatctg ctacaaataa gaatttagaa caaatgattg ataatagcca
     1501 atttagagag gatttatatt atcgattaaa tacaattcca ataaatatac ctcctctaag
     1561 agaaagaaaa gaagacatat taattattat ggaagattta attaataaga aattagtaat
     1621 cacaccagaa gcaaaaaaac ttatacaaaa ctatatgtgg aaaggtaata taagagaact
     1681 ccaaaatgtt acatcctatt taaacattat gtgtgaagat attgtattag agaaagattt
     1741 acctccaaac ttaaggtctt cagataacaa aaatacatca ttaaaactaa aatatagtaa
     1801 gaatgatata ttgaatatat tagagatact aattttaaat aaggaatcag atgttggtat
     1861 tggtagggga ttaattttaa aagcactact cgataagaac cttcaaatta cagaaggaaa
     1921 aattaaaaaa atatttgaat atctaaaaaa agaagaactt ataatttgta gttcaggaag
     1981 atatggaagt aaaataactc aaaaaggaga agatttttac aataaattaa aatataaagg
     2041 tctataaaat tataatttat caaacaaaga aatctattca ggattaaagt actccttcaa
     2101 taggtttttt tatttttcaa gtaccctaaa attataattt attttgtaca taaataatag
     2161 gaattaatag gaataggata atttttttat ttcctatttt ttttatataa actagcatat
     2221 aaaatatatt ttcttcaaaa taatatttt
//
LOCUS       DAEPOG010000295         2194 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEPOG010000295 DAEPOG010000000
VERSION     DAEPOG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.299"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            17..1420
                     /locus_tag="KR432_003621"
     CDS             17..1420
                     /locus_tag="KR432_003621"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH0642125.1"
                     /translation="MTVVALFEGEDVSTTFDKINMISRNFNSKENSIDTVTLVENNNL
                     VNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSSDVNVLNLNLNSDEIGS
                     FVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQIEKAKILANSTIIARNL
                     VNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMDAFFAVSRGSINKPRFI
                     VMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTDMAGAASVIGAMCAISQ
                     SKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNTDAEGRLTLADAIYYII
                     NNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEKAATLSGERVWKMPIYD
                     EFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHMDIAGTSTSSKSIGYKA
                     KGATGEPVRTLYYLAEI"
     gene            complement(1511..2179)
                     /locus_tag="KR432_003622"
     CDS             complement(1511..2179)
                     /locus_tag="KR432_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBH0642126.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT          887 a          232 c          388 g          687 t
ORIGIN      
        1 aagtttagaa aatgctataa cagtagtagc tctatttgag ggagaagatg tttctacgac
       61 atttgataaa ataaatatga taagcagaaa ttttaattct aaagaaaata gtatagatac
      121 tgtaacattg gttgaaaata acaacttggt aaatgtttgt cttgttggtt ttggtaagag
      181 agagttattg aatagagaaa aaattagaat cataggtggt aatttgtgta aaaaattaaa
      241 aaatatattg aaaaatgaag catataagag tagtgatgtt aatgtactta atcttaattt
      301 aaatagtgat gaaataggtt cttttgtaga agggatatta cttggaaatt ataagtttga
      361 taagtataag acaaaaagta aggattttga aagaaaagaa gttgatacta taactatatt
      421 tactgaaaaa gaagttgagt gtcaaataga aaaggctaaa atattagcta attcaactat
      481 aatagctaga aatctagtta atgaaccatc aaacgtaata tatccaaaaa cattagcaat
      541 agaaacagtt aaacttggtg cagagttcgg gtttaatgtt gatgtttatg aagaagaaaa
      601 aataaaggca ttgggaatgg atgctttttt cgccgtttca agaggttcaa tcaataaacc
      661 aagattcata gtaatgagat actttggaga taaagaaagt acagatatat taggtctagt
      721 aggtaaagga ttgacttatg atactggtgg atattcttta aaatcaaatg cttctatgct
      781 agatatgaag acagatatgg ctggtgctgc gagtgttata ggtgctatgt gtgctatatc
      841 tcaaagtaaa ttaaagaaaa atgttatagc agtagtggct gcatgtgaga atgctttatc
      901 aggaggttca tacaaacctg gagatataat tagttctatg gcaaagaaaa ctattgaagt
      961 tttaaatacg gatgccgaag gtagacttac attagctgat gcaatatact acattataaa
     1021 caatgaaaaa gtaacaaagg tagttgatgt agctacatta actggtgctg ctttaacatt
     1081 gttaggaaat gttgcaactc ctatagtaac aaataatgat gatttttatt gtgaattaga
     1141 aaaagctgcc actttatcag gagaaagggt atggaagatg ccaatttatg atgaatttaa
     1201 agatatgata aaaggagaag aagcagacct taaaaacact ggtggtaaaa atgctggatg
     1261 tataacagca ggagctttta ttggtgaatt tgttgggaat actccttgga tacatatgga
     1321 tatagcgggg acaagtacat catcaaaatc aattggatac aaagcaaagg gggctacagg
     1381 agaacctgtt agaacattat actatttggc agaaatctaa aaaacaatta taaaatataa
     1441 tttataaagc cacatggata tttttgtaat ttaagattat ctatgtggct ttatttttta
     1501 tttaagtaaa ttatttactc tttttctttt tctcaagtat aaaagtatct ctattaaata
     1561 aaagagcagg tatgtacata atactagtaa caaaaataaa cgtaaaaata gcagttgtgc
     1621 tatgacttaa aaaaccataa tagtattgtg agttggtaaa tgtgtttagt aaaaatatga
     1681 atgaaaaata aataaaacat atagctaagt tatctctaag tgcatatctg ttaactctaa
     1741 attttctaac atcaaccata tatatatctg cataaattat caagcactta gagaagataa
     1801 atataaaata tattaagcta ggaaattcat agcccaagtt tttagaaaaa atgagttctc
     1861 caactgaaca aactaaactc caagaaaaaa atatattaaa aagatgatac tgcttaaaaa
     1921 ttagtatggc tatacacaga tatatacaga tataattgat tccaattggt aaagagttta
     1981 gagtactata attacctgaa gaaataagta ataattgctc tagagccata attattacaa
     2041 gtaatgtaca aattatcttc tcaactgtat agctataagg taaaatattt ttggttagtt
     2101 ttggacaata gtataaaaat aatccaaata taaagagact tacaaattgt tcactagaaa
     2161 aaagaaaaat tttaaacata taacattctc tcct
//
LOCUS       DAEPOG010000296         2179 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEPOG010000296 DAEPOG010000000
VERSION     DAEPOG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.300"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(436..1764)
                     /locus_tag="KR432_003623"
     CDS             complement(436..1764)
                     /locus_tag="KR432_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0642127.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          789 a          364 c          231 g          795 t
ORIGIN      
        1 tttttaatac atacattata tattcatatt gtctaatttt tgttatcaaa taaaattttc
       61 taatcttatt tataaatcac gatattgatc aggtaatatt ttctatataa tacaatattc
      121 tattttatat aacagaaaga tttaattttc ataaaaccta tgtataatat ttgttatata
      181 gatatcactt agtttgaaaa aaaaacactt taaaataaag tatcttaaag caaataattt
      241 tttagatgtt taattttata attcaaaaac taaaaataaa gtgaatacct taaaataaac
      301 tttttagttc atagaacact ctttttttat aaaataaaac atctctttac ccttttaaca
      361 ataaaaaaga gacttatctc tattttgaga cagtctcttt tattttaatt ataaagcatt
      421 tgtaaactac ttggtttatc tttttattcc tctagtcata taagcataca ctggtaagcc
      481 tatagctgtt attacaagac ctcctataga tatcatagta gttttcatcc ctgcaaaaca
      541 caattgatta actacaacaa atataccact tgctatagct attataggaa caataggata
      601 taatggaact ttatactctc ttggtatatt tggatgagtc tttctaagtt tcataacccc
      661 tataaaagtt aatacataga atatccaagt agcaaataca gctaagtcag ttaataaatt
      721 aaattgacct gacaaagcat atatagatgc aataacagat actaatgcta ttgcatttgc
      781 tggaacttga gcagaattta atttagaaaa tataccatac tttggtaaag tcttatctgt
      841 agctaaagta tatgccaccc ttgaaccagt tagtaaaaat ccatttaatg ctccaaacac
      901 tgaaattaat atacctacag atattatttt tccacccatt gaaccaaata taacttcagc
      961 aactgctgat gcaggagaag cataatttgc taattcatta gctggtaata cccaaagata
     1021 cgctaaattt attataaaat aaacagccat aacaactgac aatccaccta ttatagcttt
     1081 tggaaggtct tttccaggat tcttcatttc tccagctaaa gtaccaacat tcatccatcc
     1141 atcaaaagca aataatatag caattaaaac ttgacctagt acactaccca ggcttaatcc
     1201 ttcaccaacc ataggtgtaa gtattggatt atttccacca cctttaataa atccaacaac
     1261 cattattaaa ataagtggta ttaacttaca aattgtagag acagtttgta tgactcctcc
     1321 agtttttgaa cctaacatat taagccctgc aactattact ataattccaa ttgctattgg
     1381 aagtaaaagt gatgggctac caattagtgc tgatgcttgt tctccaaata taacacctaa
     1441 tgctgccatc atccctggat aaaataatac aatttgcatc catccagtta aaaaacctaa
     1501 tttgtctcca taaatttctt ttatatatac catcattcca ccagtttttg gtatcgcaac
     1561 tgatacttct gcagctgtaa gaccagctgt tatagttatg attccagcta ttaaccatgc
     1621 caatatacca agacctggag ctcctccagt taatgtatag actgcttgtg gtttaaaaaa
     1681 tacgcccgct cctataacag aaccaacaac agttgataat gctgctgata cgccaagagt
     1741 ctttttcagt tcattacttc ccattctttt cctcctcatt aatcctattt aattttaatt
     1801 tcatagttaa ataagcatat attatgccaa tatatagaaa aatagctttt ttatttatta
     1861 aaataaaata aactctactt agctttcgta atcttttact tttagttatg tttaaatata
     1921 aaaaaattat aaaaaccaaa aatccaaata taataatatt tataaataaa cgtatttttt
     1981 atattgtata atatacattg tatatttgta acactaaaaa tagattataa ctattaagtt
     2041 aaattgattt tgattttttt gcaataaaat ttcatttttt tcaaaaacat tttggcttct
     2101 ttctgtaatt ttctatcttt aaggtctttt atataatagt atgatttttg cttgttttat
     2161 agtctatttt atattaata
//
LOCUS       DAEPOG010000297         2066 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEPOG010000297 DAEPOG010000000
VERSION     DAEPOG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.301"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..841
                     /locus_tag="KR432_003624"
     CDS             <1..841
                     /locus_tag="KR432_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0642128.1"
                     /translation="FRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMKDL
                     EFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEEDR
                     IKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTVNQ
                     LSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTSEI
                     ETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIKSN
                     ESN"
     gene            914..1126
                     /locus_tag="KR432_003625"
     CDS             914..1126
                     /locus_tag="KR432_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBH0642129.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            1327..1926
                     /locus_tag="KR432_003626"
     CDS             1327..1926
                     /locus_tag="KR432_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642130.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
BASE COUNT          911 a          194 c          357 g          604 t
ORIGIN      
        1 ttttagaaaa gcaattgctc actctataga taaagataga attattagtg aaggttatat
       61 ggatgatgct actccagtta attttccact taattcaaaa tcaaaatact ataatagtga
      121 aatgaaggat ttagagttta ataaagataa ggcgatagag tgtcttgcta aggtagaata
      181 tgcaaatata aactcagtaa atcaaaatga caataaagta aaaaatcaga aaaaatcagc
      241 taaaaagaac tcaaagaagc ttacgccaga agaagaagca aaagctaaaa aagcagaaga
      301 agatagaatc aaaaaagaag ctgaaaagaa aaaaaataga gaaaaagagg aagtaaagaa
      361 aagtctttct gaaatgaact taaagataat agtaaacaga gataatagtg agagaataaa
      421 aactgcaaac ataataaatg aaaacttaaa agctatagga ataaatagta cagtaaatca
      481 gctgtcagat aaggatatgg aaaatgctct taattctaaa aattatgatt tagcattagt
      541 tggatggaaa ctatcaatta taccagatgc aagtagtatt atagcaagta gtggatatac
      601 tgatgataag ctaaatggat atatgtctgc acttacaagt tctacgtctg aaattgaaac
      661 aaaaaaagca tacaaagatg tacagacata tataaaagat aatgcaacat ttataagttt
      721 agctgtgaga aacaactata ttgtaagtaa tagtagatta aaaggtaaaa ttactccaaa
      781 tgattttgat gtatatgagg gaatatctaa tttagatata aaatcgaatg aatcaaatta
      841 aatgattaat ttctatttaa aaagtataaa ctatatgata gaataccctt atggggtata
      901 aggaggtaat taaatgaaga agaaactatt agtagaaggt atgagttgtg gtcattgtgt
      961 aaaccatctt aaaactgcat taacagaaga tatagatggt attgaagttt tagatgtgga
     1021 tttagaaaat aagtgtgcat ctgtagatat gaaagatgat atatctattg aaaaattaaa
     1081 agaagtgata gcagaattag gatttgaact aaagggaatt gaatagaaat tgataaacta
     1141 aaaaaaggcc tctattgggg gagtaataga ggtctaaaaa tgggggagat tgagtatgtt
     1201 tgattttaca ttactattat ctcccctttt aaattaatat atacataaaa agtaaaaaaa
     1261 ttttgtaatg actcacattt cttgagcttt ttgaatatac tataaatagc taagggaggg
     1321 agtatcatgg aatctagggg atataaagag gatttaaaaa aaatgatact tgatatggcc
     1381 cacaaagaac ttgagagtta tgtatcacaa aaaggagcaa aaaaatactt tgaaggtaac
     1441 ttggataaga ttattaataa taatataaaa aaattcaatc aaagtattgg tcttaataga
     1501 gtatatttaa aaatgttaaa aaagtgtgca tatcaagaaa gtgtaacttc aatatcaagg
     1561 actataaaga aagaagcatt atttaaatca aaaaaagagc tatttgcttt ttcagagtac
     1621 ttaaaacttg atattaacaa gcgactttca tataatcaaa ttttaagaaa aatatcaaga
     1681 tatatatact ataatagaag ttcatatgct caaaaatacg ttgttttcaa aagaggagat
     1741 gaagaatatc ttttagaacc agagaaaata aaagatgaat taatatcaag ctataggtca
     1801 aagactagag aagatatgag gtcaatagca aaacttttgg atatagaagt agaagatgat
     1861 tataatgctg aagatattag aaaaaaagta ataaattata ttatcaaaga aaaacttaaa
     1921 agttagagtt ttaaagaata agttctgcca atttgcatga ttaagtataa cttgggtata
     1981 aattttttgt gcatttgaaa gagcttattc tttttttgac atattctttt aaataattta
     2041 ccaccatttg gtaattggtc atcatc
//
LOCUS       DAEPOG010000298         2053 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEPOG010000298 DAEPOG010000000
VERSION     DAEPOG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.302"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(242..1669)
                     /locus_tag="KR432_003627"
     CDS             complement(242..1669)
                     /locus_tag="KR432_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBH0642131.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            1860..>2053
                     /locus_tag="KR432_003628"
     CDS             1860..>2053
                     /locus_tag="KR432_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642132.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEK"
BASE COUNT          683 a          340 c          230 g          800 t
ORIGIN      
        1 gcgagagtgg ctttttgctt ttttgaatag tttactgatt aagtaaacta ccacactagc
       61 tgttaaacta gctaaaacat taaacaaaaa gttatccatg aactcacctc ccttcgaatc
      121 gttgggagga taatcttttg tacatgaact ccactctata gattgtaagt taaatcttct
      181 tgctaaaaat attataacat attatggtaa attatataat tattgtttaa aagttctaat
      241 tttaattata aattttccca gtttctatat ctataaaaaa ccaaccaaaa gtaccattac
      301 tattttttga attgatttta acagcataga cattaccact atatatatca tctctaaaat
      361 ctatcatatc aactactaag ttattgatac tgactttatg tctcttagca cattctttta
      421 atgctatctg ttttgcttgt tcctctgtta tacttccttt atttttataa tcatctggat
      481 tacatacttt catattacca ttaacatcat atgtataaac tttgctcata tcatttttag
      541 ctacacagta tctaacatca ccctctgcac cctgtttaat gtaagcaaat acatagtact
      601 cttttcctga aaaatcaaaa tttttatcta gtagaaatat taaatcttca tcttctgaat
      661 aattaggttc gtaataaaat ttcttcacac ctaatagttt tttcactgca ttagcagcat
      721 cttctactgt atattctctt ccagtattag aatcgcctat attttttgtt acatttaaac
      781 actgctgtaa tatactatca ctcaaatttc ctatagcagt taatttaatt gtacttttca
      841 atacactttt attactatca ttaccaacta acacaatagc attattttta gcaatggtag
      901 aaccaacaag agcatacact aaatcagtac cacttgctat ataaaattct tttactccac
      961 tataaaattt atttattatc tttttgttag tatcatatct atctgtccca cctaatctag
     1021 ttgaattagt atccttaact agtttgtcat tcattgatga agtaccacct attacataac
     1081 tttctacacc agttgtatta aatggaacac tttttccatc agttaatact atagctgctt
     1141 tatctctaac tgctacaggt gcagcactca ttgcatcagg ctctccttta aaagcattag
     1201 tcaatatgac tttatttact ttgttaattg aatttatttc ttttgcaaca ttatagcttg
     1261 tcttaattct atcactacct tgaagtcttt taatctctat tcctttgcct tttagaacag
     1321 tttcagtata tttattaata gagttttctc ctccaattat atatactttt tttgcctttt
     1381 ctaatctttt aagtgtagca tttggtatgt tatttttctt agtcaaaaga attggagcgt
     1441 ttatagctcc tgcaagtcca cttgcactta atccatcagc cattgtactg tctgcattaa
     1501 ttaatatagc tgtagtatag ttttgtttgt ctgcaattat accagcagtt tcgtatttat
     1561 ctgcaccttt aattgattca attttatcaa gagcatttgc agatataggg caactagcaa
     1621 taaatataga taaagataac cctattgtta ataatttttt aattttcata atatctcctt
     1681 ctcttaaata ttttcaccta aattatataa tacttttcag gaaaatgtta ccataaattt
     1741 taaaaattat tattacttaa tataatatta atgaaaaata tcattcttaa attaatgact
     1801 ttggtagaat atggtgataa aataaaatta gtgagagaca atataagggg gattatatta
     1861 tgataatcag taaaaaaata acagcaatat tagtatcatt tttaattatg atatcaatgg
     1921 ttggatgtgc tccaaaagaa agtgctacag aagtagctaa aaagtacctt gatagtatag
     1981 aaatcaaaga ctacgaagta gcatataatt tattaagtaa agacagtaag gaaagtgtca
     2041 gtctagaaaa att
//
LOCUS       DAEPOG010000299         2040 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEPOG010000299 DAEPOG010000000
VERSION     DAEPOG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.303"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(84..974)
                     /locus_tag="KR432_003629"
     CDS             complement(84..974)
                     /locus_tag="KR432_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0642133.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1123..1983
                     /locus_tag="KR432_003630"
     CDS             1123..1983
                     /locus_tag="KR432_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0642134.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          711 a          282 c          254 g          793 t
ORIGIN      
        1 tttatataaa tttttttatg ttacttttca aataacagtc atactaaaac aatttcatca
       61 tataatttga aaattattac tatttacaca gcttttatca tcatcttacc accatcaaat
      121 atatattcaa ccatattatt ctcatataaa taagttatat aagaacgtat tgtacttcca
      181 accaacacat attgattagc atcaagcttg acattgtaca aatcacacat tttttcaata
      241 acttcatcaa tcataactgg ctcataacaa acctttttaa tattatctat tatttcttcc
      301 atcttcttct tattaatatt aatcaaactt tttatatctg tagttttgac agagtgactt
      361 ggaacaaaga aatcagcctc taacattctc aatttttcta gtgtcttaaa ctgtgaatct
      421 atatccagta ggaagaaaaa atgatattta gtaattatat tctctggtgt aagactatct
      481 gcaacaaaca aaacattatc tggagttcta acaccaatca tttcaaaata atgacctgga
      541 agagaaatcg cttcaagttc tgtatctaca attttgccat ttgaaggtat aatataatca
      601 acttcacttt cctttgctaa taaaaattta ttcttcagtg ctttacttgg atagccacca
      661 tataaaaatg ctgattctaa tattggattt tctgtaaaat atccttcaat tttagtcgta
      721 gcaatcttgc attttgtttg attcttcaaa tatgcatttc ctcctatatg gtctgcattt
      781 gaatgagtgt ttattattaa tttaggactt aaattatgtt cttctaataa tcttaaaact
      841 tgtctagctg tatccttatt atttccactg tcaattaaaa ttgcatcttt accatctacg
      901 tatacaccta ctattgtagg gagtggaata taatatgtat ttccttttat atgttctaga
      961 gttaatttag ccattttata acctcccttt aaatttactt ttaattatag cattaatttt
     1021 ctgaaaattc caatcaaatt gtttttataa tatattttta tttcttttat taaaaattgg
     1081 aaaaactata taatatatat tataatctat atataaggag tgatgttatt gaaaaaagat
     1141 agaataactt tttcagaaat tttaatatta attgtaggct gtatattaat ggccatttct
     1201 cttaatttat tttttaatcc acatgcaatc gctgctggtg gtataacagg acttggagtt
     1261 gttttaaatt cattattcgg tgtagaactt tggattgtca atttactttt aaatgttcca
     1321 ttattcatat ttgcttacaa aatactttca aaaaaagatt gttttaaaac ggttttagga
     1381 ataatttttc ttaccatagc acttaagctt actgcaaata tggctacatt agatataaca
     1441 aatgatatgt atcttgcaat tatatctggt tctattttaa tgggagttgg tcaaggactt
     1501 atttttagaa taaatggctc tacaggtgga actgatttaa tggcactcct actaaataaa
     1561 tattttccta catttagtat acctgtttta atgggtattg tagactgcgt tgtagttgtt
     1621 ttatcaggta tagtaaatag acaggttgaa attgcacttt attcaactgt ggctttatac
     1681 attttagtaa aagttagtga cttgcttatt gaaggattta attattccaa atcattcact
     1741 attatatctg acctttcaaa agatataagt aaaaaaataa tggaagattt agatagaggt
     1801 gcaactattc taaaaggaga aggtgcatat actggagaaa ataaaaatgt acttttagta
     1861 gtagttgaaa agaaagaagt tgtcgaatta aaaaaattag taaaaaatgt cgaccctaat
     1921 gcttttatta taattacaga tatacatgaa gctttaggta atggatttaa aaaaatagaa
     1981 taactaacaa taaatagcaa gtataattaa catatacaaa ttcaactttt tatatgttat
//
LOCUS       DAEPOG010000300         2002 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEPOG010000300 DAEPOG010000000
VERSION     DAEPOG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.304"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            165..611
                     /locus_tag="KR432_003631"
     CDS             165..611
                     /locus_tag="KR432_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH0642135.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            632..1675
                     /locus_tag="KR432_003632"
     CDS             632..1675
                     /locus_tag="KR432_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH0642136.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          833 a          201 c          354 g          614 t
ORIGIN      
        1 tactttgtaa caattttaac aaagtatgtt gatataatac tattaaatta atcttagatt
       61 agtttattct attgatttgt agtttaaatt tatattcaaa aacattgtat aaacatataa
      121 tttatatagg gttttataat tcatatgaaa agagggtaga tagtatgaag ttaatatggt
      181 tgatagtagc agtcttattt ggaatagcag aaatgttaac accaagctta acattgatat
      241 ggtttagtgt aggagctgta atacttatat ttttaagtag ctttatagag agtattttct
      301 tacaaatttt aatatttgca gtaatttcta tagcaatgtt agtcgtagct actaaaaaaa
      361 tagtaaaaaa agataaaggc tataaatcta acactaattt acaagcaatg atgtctaaaa
      421 aaggtattgt tacagaagag atttctccaa ataacacagg tctagttgta gttgaacatg
      481 aaacatggac ggctatttct atagatggtg aaaagataga aaaaggttct acagtagaag
      541 tattaaaaat tgaaggggta aaattagttg ttaaaaaagt agacgccaca gttaatgtga
      601 caaaccaata gaataaagga ggatttatag tatgggtact aaaattgttt taagtattgt
      661 attaatagta gtagtcgtag caataagttt aacgtgtata agagttataa aacaatctaa
      721 agtaggtata ataatgagac ttggtaagtt tcaaaaagtg gctgaaacag gtgtacattt
      781 cttaatacca tttttagata aaatggcata tgtaattgac cttagagaaa ttgtaataga
      841 ttttccacct caaccagtta taactaaaga taatgtaact atgcagattg atacagttgt
      901 atattataaa gttacagatc cagttagata tgtgtttgag atagctaatc caatagctgc
      961 tattgaaaat ttaacagcta caacactaag aaatataatt ggtgaacttg atttagatga
     1021 aacattaaca tcaagagata taataaatgt aaaaatgaga actatccttg atgaagcaac
     1081 agataaatgg ggtataaaag taaatagagt agagttaaaa aatataatgc ctcctcagga
     1141 tattcaagtt gcgatggaaa aacaaatgag agcagaaaga gaaagaagag aggcaatact
     1201 tcaagcagaa ggtaataagt cagctgcaat acttcaagca gaaggtgaaa aacaatctgc
     1261 gatattaaca gcagaagcca aaaaagaagc tatggtacgt gtagcagaag gtgaaaaaga
     1321 atctgctata ttagtggcag aaggtgaagc tgaagctata agacagaccg ctattgctaa
     1381 ggcacaaggt gaggctgaaa tgataaaaag aactcaaatg gcaacagcag aaggtttaaa
     1441 attagttttt tcagcaatga aagaggctga tatagacaat aatattctag cattaaaatc
     1501 tatggaagct cttgaaaaaa tggctgaagg taagtcaaca aaactggttt taccttcaga
     1561 agcagttaat ttcctaggaa cattcaaagg aataaaggaa gttatgagtg atgataacaa
     1621 agaagtactt gatataaaag aagttttaaa tgataatgaa tcattaaaaa aataaaagag
     1681 ttttgttatc ataggaagga tatggaaatt aaaggtcgaa ttaattaaat atagcacaaa
     1741 aatgcacgtt gaaaagaaga ggacttataa atttttaagt cttcttcttt gtttttatta
     1801 gaaagatgaa attataaaaa tgaattaaca gaatctttat acaatttatc ctttactaat
     1861 gtaaaaagtg ttatataata ttatataaaa catatagcaa ataagcatgg ttttagttag
     1921 tctatttata ttattaatat atatttataa aaatataaac attaatacta agaaaactaa
     1981 taaataattt aaaagtttat at
//
LOCUS       DAEPOG010000301         1890 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEPOG010000301 DAEPOG010000000
VERSION     DAEPOG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.305"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(197..1468)
                     /gene="serS"
                     /locus_tag="KR432_003633"
     CDS             complement(197..1468)
                     /gene="serS"
                     /locus_tag="KR432_003633"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0642137.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(1593..1799)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          595 a          348 c          185 g          762 t
ORIGIN      
        1 ataattttta gatattcata caagagtgtt ttataaaaat atttagtgtt ttatgatgaa
       61 aattctgctg tgtaaattaa cataaaaaaa ctatgaaatt aatatttata ataatttcat
      121 agttttttta tttattgttt actcataata tattctcttc atcaaagcat actctacata
      181 tacaattaat ataaatttat tttataacat caacacccat ataaggtctt aattcttctg
      241 gaactactac agaaccatct gcctgctgat aattttctaa tatagcagcc aaacatcttc
      301 ctatcgctaa tccagaacca tttaatgtat gaacatattc tgcttttgat tttttatctc
      361 ttttaaatct tattcctgct cttcttgctt ggaaatcttc aaaatttgaa caagaagata
      421 tttctacata tctattgtaa cttggcatcc aaacttctaa atcatattta aatgcagctg
      481 taaatcccaa atctcctgta catattttta caactctata tggaagacct aacatttgta
      541 acatagtttc agcatcatga gttaatgatt ctaattcatt ataagaatct tctggtttaa
      601 caaatttaac taattcaact ttattaaatt gatgttgtct aactaaacct cttgtatctc
      661 ttcctgctga acctgcttca gatctaaaac aaggagtata tgcacagtat ttaattggaa
      721 gctctgcaac atctaatatt tcattagcat gtatattagt aacaggaact tcagctgttg
      781 gtattaagaa atactctaat ccttctattt taaacatatc ttcttcaaat ttaggaagtt
      841 gaccagtccc tataaaacta tttctattgg ccataaatgg aggtaagact tctgtatagc
      901 catgcttagc tgtatgagta tttaaaaaga aattcattaa agatctttct aatctagctc
      961 ctaatcccct atataaagta aaccttgacc cagttatttt ccctgctctt tcaaaatcta
     1021 aaatacctag acctgtccct atatcccaat gcgctttata ctcaaaatca aatttagtag
     1081 gttctcccca agttcttatt tgaacattgt cttcatctgt ttcaccttgt ggtacctctg
     1141 gatgaggaac atttggtatt ctcattaatg tatattccat ttttgcttct acttctttta
     1201 ctttctcatc aatacctttt atcttatctg ataactcttt caatcttgtc ttttcttctg
     1261 ttacatcttt tccttctttt attaattgag gtattttttt agaatttaca tttaactcat
     1321 ttttcataac ttcaacttct tgaagtattt ctcttctttt attatctagt tccactacag
     1381 catcaaggtc aaattctctt tcacctcttc tttccatagc ttttttgata tcatctagat
     1441 tttctcttat tcttttaata tctaacatat taattcctcc taataatttt gataatataa
     1501 attataattc caattaattt acaagtattc tagctgtaaa cttttaattt aaacaaattt
     1561 gaaaaataaa aaaagacctc cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt
     1621 tgccacccta attgattata ctaaaacgta taatcctctc agacctataa cgtaggtaaa
     1681 ccgtctagct ttaactagaa gctctagagt agattcaaaa accattgtaa tgagttctca
     1741 ccatccactc attctctgaa tacaatctgt tttttactat tttccttcac cgctttttaa
     1801 ctaattgttt tatttgataa tcattatatt atatatatta acatctttca atactattat
     1861 tcaaattttt ttagaatttt ctttaatttt
//
LOCUS       DAEPOG010000302         1885 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEPOG010000302 DAEPOG010000000
VERSION     DAEPOG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.306"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            13..900
                     /locus_tag="KR432_003634"
     CDS             13..900
                     /locus_tag="KR432_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0642138.1"
                     /translation="MIIRIILFRDVPIYAIFKGTFRNVNLIPFYTIYQFIVDSNLDFM
                     KATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIVIILVSLAFELTQYIFALGSSDID
                     DVILNSLGGIIGINIYINMNKLFPNDIKRFKAIIATSLVLGVLGLGVISKNYHNLLTF
                     KFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKRPENL
                     IDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWIDKEN
                     QPKDKHGIMASKLLIGLYE"
     gene            1015..1800
                     /locus_tag="KR432_003635"
     CDS             1015..1800
                     /locus_tag="KR432_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0642139.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
BASE COUNT          801 a          153 c          278 g          653 t
ORIGIN      
        1 gtatatattt taatgattat aagaataatt ttatttagag atgtaccaat ttacgctatt
       61 ttcaagggaa cgtttaggaa tgtaaattta attccatttt atacaatata tcaatttata
      121 gtagatagta atcttgactt tatgaaagct actgtaaata ttatagaaaa tataggtata
      181 tttattccta tgggaatttt tttacctata gtatgtaaaa atctaaataa aaaaactata
      241 attatagtaa ttatacttgt tagcctagca tttgaattaa cacaatatat ttttgctctt
      301 ggaagtagtg atatagatga tgttatttta aattcattag gtggaataat tggaattaat
      361 atttacataa acatgaataa attatttcct aatgatatta aaagatttaa ggctataata
      421 gctactagtt tagttttagg agttttagga ttaggagtta tttctaaaaa ttatcataac
      481 ttactaactt ttaaatttag acctgataga aaaatatcaa aaatattaat tgaagaaaat
      541 agggaaataa ttaaagatat aaataaagat acagtagata tagttgggac atttgagagc
      601 tttaaaaaag gaataataac tattaaagca ggttctaata acaaagtaaa gagaccagag
      661 aatttaattg attctgatgg aaatataaga atttatttaa atgaaaatac aaaattagta
      721 tcttatataa ttaccaaaga atcaaaaatg gatatagtta aatatgagga atttgatgtg
      781 aagaatttag atttattaag gagatatgat actattaatg tttggattga taaagaaaat
      841 cagccaaagg ataaacatgg tattatggct agtaaattat taattggatt gtatgaataa
      901 aatattaaac aagaataaaa gaataacaaa tataaaaaac gaaaaaacta tttctataat
      961 tatttttacg ttgtgcataa aataagaaca ttagtgaagg agtagagata aattatggag
     1021 ataataaaat atatcttgta cttttttata tattcatttt taggatggac agtagaaagt
     1081 ataggatgtt ctatagcaag caaaagaata atcaacagag gttttttaaa tggtccaata
     1141 tgccctgttt atggttttgg agcagtaatt gtgatatcac tattaggaag atttaataat
     1201 gtagtaatag tatttctatt aggaatgatt ttaactacga tactagaata ttttacagga
     1261 tttattttag aaaccttatt tcatgctaaa tggtgggatt attctgacag aaaattcaat
     1321 ataaagggta gagtatgttt aaaaaatgca atatactttg gtgtaatgtc tgttttaata
     1381 ataagattta tacatccatt tataaaatat tttgtaagta taattccata tagaatatta
     1441 atttcaatgg ctataattac aacactttgg actatacttg atttgattgt tactattata
     1501 acacttaaaa aattagatat aaagttaaac ttactagacg atataattac agaccttaat
     1561 gatataaatg tgaaattgga taaatttgat agaggagaaa tacaagcttt atttaaaaca
     1621 ataaataacg acggattaga agttagagaa aaaataaaca aaataaatag taagttggac
     1681 cgtatagagt ctaatattat tttacaaaaa agagttataa aagcatttcc acatataaaa
     1741 cataaaaaac aacaagagca actagaacat tttaaaaaaa ttattaatga gaaaaggtaa
     1801 ttatacaatt taatgaagac gaatagaata atggtattta taagattatt ttcagaggac
     1861 tctataaaaa tagaatagag tcctt
//
LOCUS       DAEPOG010000303         1884 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEPOG010000303 DAEPOG010000000
VERSION     DAEPOG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.307"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(100..1119)
                     /locus_tag="KR432_003636"
     CDS             complement(100..1119)
                     /locus_tag="KR432_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0642140.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1295..1834)
                     /locus_tag="KR432_003637"
     CDS             complement(1295..1834)
                     /locus_tag="KR432_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0642141.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
BASE COUNT          652 a          332 c          167 g          733 t
ORIGIN      
        1 tgacatttta acacttggct aagtatacac cttaccttaa cttaaaatta aatttattta
       61 agaatatata ttataaattc ttatattttc taatataagc tatatactat cagctggata
      121 aacaatacta ctttgtcttc ttatctcatc cataatttcc attactatta ttgaattttc
      181 atgagaattt atatttgatt ctattttatt gttatttata agacttataa actctacaag
      241 ttcataatac attccttcta tttctctagg cttttcctct gctttttctt ttggaataga
      301 tatatcttct tctcttccat ctctgtattt aataatagct ttttcaaata gattaagctt
      361 ttcaattatt atacttccct cttctccttg tatttcagat ggtatataag aatcagctac
      421 tttagaatac tgaattattg catccatatc atcatattga aatacagctg aaccttctcc
      481 atccactcca gtttgaagta tatgagaaac tgcctttaca gtttttggag caccaaataa
      541 attcaccata ggatgtatac aatacactcc tatatccatt aaagctccat ttgacaactc
      601 ttttttaaag gcattttcta taattccatt tttatattta tcatatcttg aagaatactg
      661 acaatagctt ccaaaaaatc ttcttatttt ccctatttta tacaagtttt ctttaactgc
      721 tttaaagttt ggaacacatg taattctcat agcttccata agcaatacat tattatcttt
      781 ggctgttttt atcattgatt tgacttcttt taaattagat gctaacgact tttcacaaag
      841 cacatgtttt ttgtgtttta aacaagtaat ggcttgcttt gaatgtaatg catttggaga
      901 agctatatat acagcatcta tatccttcga tttagccatt tgttctaaat catcaaaaaa
      961 aatacttgct ccatgtagag ttccaaattc tctagctttt tctaaatttc tagaatatac
     1021 agcaaccaat tcaaaatctt taaccctaga tgcagctctt agaaatatat ttgctatatt
     1081 gcttgtccca acaataccaa atcttatttt acccatcata aatcactccc cattttctac
     1141 actttatttt aattttaata aaaaacttta caacttatag atatattata aaataatttt
     1201 atcaaataaa aaagaaagct aattttaaat gatttcacca ttttaacata gctttctttt
     1261 aactacaatc taaattcaac ttatattttt aaagttatct ttctactata tctttatttc
     1321 ccctcaaaaa ttttatagta agaatttcat ccacaaacaa tgttactaaa agtatagtta
     1381 caagaattgg ccaataaggt attataagac tatataaact atcaactata tacacttgaa
     1441 aatatctaac tccaataaat gtcaatatag tccaatatac agaaaacttt agacatacta
     1501 ttcctctact attgtactta taatctgaat aatcccaata gaccttatta aaatatactt
     1561 tcataagtat tcctgtaata tattctacta ctgtcggcac tactagacat aataaaagca
     1621 atgcaaatat attattgggg tcaataaatc ttgagacctc tattataatc gacattgcaa
     1681 ttgcatacat tggtttaaat ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt
     1741 tataataact atataagttt tcaattatcc aacctataaa accatacaag aaaaaattaa
     1801 ataaaaaata taatagagaa cctacattat ccatgaatat tcctcctagc tgtttatact
     1861 tattttttct catattcatg gatt
//
LOCUS       DAEPOG010000304         1860 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEPOG010000304 DAEPOG010000000
VERSION     DAEPOG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.308"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            123..569
                     /locus_tag="KR432_003638"
     CDS             123..569
                     /locus_tag="KR432_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0642142.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            571..1665
                     /locus_tag="KR432_003639"
     CDS             571..1665
                     /locus_tag="KR432_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBH0642143.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1753..>1860
                     /locus_tag="KR432_003640"
     CDS             1753..>1860
                     /locus_tag="KR432_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0642144.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
BASE COUNT          760 a          178 c          331 g          591 t
ORIGIN      
        1 gttttttaaa gaattaataa aacatagatt tagagaataa aatattcttg actatattaa
       61 ttgtttaata taatatagtt gatatagtca aacatattat aattatagaa gggaaattta
      121 agatggattt taaaaattta cagtatgaaa gtttgtcata tattatttgt aacttgcaaa
      181 aaaactttaa actttattgt gaaaaatgtt taaaaccata taaacttacg aatggtctat
      241 atttctattt aatttatatt aataaaaata ggaattgttc tttgaatgat gtttctacag
      301 aatttgaagt tgataaagct catacaacaa gaactatatc taggttagaa caagatggat
      361 acatagaaaa aatccaaaac cctaatgata gtagagcttt tcagttgaga gtaacagata
      421 agggtgagga agtattaggg gatataaaaa acatattttc aaagtgggat aaccacataa
      481 aaaaagagtt ctcagataca gaatataaag aactggtaaa gaatttacat gtggtaaagg
      541 atataaaaac agctgtggag gaaggatagg atgtattgta atttattaaa accaattaaa
      601 attggaaatt tggaattaaa aaatagagta tcttttgcac ctacaagcat gggtcttaaa
      661 ttggaagaaa aaattaaaaa gttttctgat attgctaagt caggagtagc tctgattact
      721 ctaggagatg tatctattag acctagtttt cataaggtag ctataagcct atcggatgaa
      781 gatggagtca ttaaatacaa gaaaattgta gatgaaatac acaattctgg tgcaaaggta
      841 tcagcacaac tattttgttc agattatgat gttaatttaa ttaaagatac tatgaagatg
      901 ggaattactt cacatgatga gataaaaaaa ataatgaatg acggagtaaa agattacata
      961 acaaatatgc ccaaagaaga aattaaaaat attataaatt tatttaaagt aacagcattg
     1021 aatgcaaaaa aagcaggttt tgacatgatt caaattcatg gtgataggct tgtaggaagt
     1081 ttttcatcaa gtatatttaa taatagaaat gatgaatatg gtggaacgtg tgataataga
     1141 agtagatttg caagcgaaat aattagtagt atccgagatg aagttaagga cattccaata
     1201 gactataaat ttgcaataag acaagaaaat cctcattatg gaaatgcagg agttttactt
     1261 tcggaagttg agtattttgt taaaaaattt gaatccctag gtgtaaacag ttttcatgta
     1321 acacttgcaa atcattcaaa attagaagat actataccaa ccaataatca tccttacttt
     1381 aaagatgaag gctgtttttt atatttagct gatgaagtaa agaaacacac aaatcttcca
     1441 gtatgtgggg ttggaaaatt gtctagccca gattttatag aaagtattat ttcaaataat
     1501 agagtagata tggtttctat gtcaagacaa ttactagcag atagtaattg gttacagaaa
     1561 gtaaaagatg gaagagtaga tgagattaaa aagtgctgtt attgtaataa gaagtgtgca
     1621 gatgctttac aaacacgctc tcagtttgga tgtatactag attagaatat ctttataaaa
     1681 ataaataatt tgttaaggtt aatatatttt tggtgattta gataagtatt tagataaaga
     1741 ggaggagaca tcatgaaaag tttagtttta tattcaagtt taactggaaa tactaaaaag
     1801 attgcttatg ctatatatga tgaaattcaa gaagaaaaag acattaaaga tgtaaatgaa
//
LOCUS       DAEPOG010000305         1846 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEPOG010000305 DAEPOG010000000
VERSION     DAEPOG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.309"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            269..>1846
                     /locus_tag="KR432_003641"
     CDS             269..>1846
                     /locus_tag="KR432_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH0642145.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          735 a          147 c          357 g          607 t
ORIGIN      
        1 aaaatataaa taagtcataa aagattataa aaataaagag atatttagat aattagtgta
       61 aataggtttt aaatcaacat aataacttaa attatatggt ataataaatt aaataattgt
      121 tttaattcaa aattttttgt tattttaaat tatttcaaaa ctcttagttt tagtgatttt
      181 atttatgata tttatgggat taaaagattt caatagatat ttttggtaag tatttaagaa
      241 gtctttaact ttataggagt gataatttat gctaataggt acatctaaat taagcagaaa
      301 tgttgatact gcatgtgtag aagaagtagg gattccaatg attgtattga tggaaaatgc
      361 ggttatatct gcaatgaaaa atatggatat agacatgtat aatagttata cagttgtatg
      421 tggtgttgga aataatggag gagatggtct tggaatagca agacatctaa atacatatgg
      481 aaaagaagta aacgtatttt tagtaggtaa tcttgaaaaa ctaagtgaat gctctaaaat
      541 aaattacaat gtacttttaa atatgggaat taatattata aatattaata aaaatgattt
      601 aagtgaaaaa aattatggag atgaaaatga agtagataaa attattaaag ttgagaaaca
      661 aaatattata aaaaataaaa atttaacaat ttttaaagat agagtatcta aaagtgatgt
      721 tgtagtagat gctatatttg ggactggatt aaaacgtgat ataactggta tttttaaaga
      781 agttattgac atagtaaacg ataatagcaa aaatacatat tctatagata ttccttcagg
      841 tatagatagc gataatggaa atgtattagg aacttgtatt agagcaaaca aaacgattag
      901 ctttgagttt tataaaagag gttttctaaa ctatgacact agcacactta taggtgatgt
      961 agttgtagaa aagataggtg ttccagattt tattacaaga aaataccatg atgaagagtt
     1021 cataactgat aagacttttg taaaaaataa tattagaaga agagataagc atggttttaa
     1081 aagtgatttt ggaaaagtat ctatagttgc aggttcaaaa ggattttatg gtgcaagttt
     1141 catagcaact gaatctgctg ttaagagtgg gagtggactt gtaacattaa ttagtagtga
     1201 agatgtgcaa gaaaaggtat ctattagact tacagaagca atgactttaa attttgaaga
     1261 agaaagatta aataaattac ttaattctag tgatgcaatt ggttttggtc caggtatggg
     1321 agataatagt caaacatttg ataaattact taagatagta gaaaattcta attgcccaat
     1381 agtattagat gcagatggat tgaacgttat gaaggacaga tgttataagt ttttagaatg
     1441 gaaaaataga tttgtaatta cacctcattt gggagaaatg gccaggttga caggagattc
     1501 aattgggtat ataagagaac atagagtaga tgttgcaaaa gaatttgctc aaaaatataa
     1561 tctggtggtg cttttaaaag gttatcaaac tgtcataaca gatggtaaaa aaacatatat
     1621 aaatcctaca ggaaatagct gtatggcaac tggtggaatg ggtgattgtt tgcttggaat
     1681 gattacttca tttataggtc aaggtatgga tatattggaa gctacagtta gtggagcata
     1741 tatacatggc tatataggag ataaattgag caaagcaatg tatacggtaa atgctacgga
     1801 tttaataagt aacatttcat taacaatgaa agagttgttg gatata
//
LOCUS       DAEPOG010000306         1832 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEPOG010000306 DAEPOG010000000
VERSION     DAEPOG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.310"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            338..1774
                     /locus_tag="KR432_003642"
     CDS             338..1774
                     /locus_tag="KR432_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBH0642146.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          773 a          176 c          295 g          588 t
ORIGIN      
        1 gtgaaatttt gggtgaatta aataagcgaa actataatag aaatagaata tatggaaaaa
       61 tacatctcta tgcttaaaaa agaaaaataa atctattaaa ataacataat aaaaaaaata
      121 ttaagaataa aaatataatt ataagtattt atacaaatct agttgatttt taatataaat
      181 gtaaatacaa ttatagctaa atgaataatt gtattaatat ttatttgtaa tatgcttaat
      241 ataatttgca attttacata agtttgttaa gtacataact aatttaagaa tatgtattta
      301 tatattaaaa atttattatg agggggacaa ttttattatg aaattctata aaagaatatt
      361 aacattgact ttaagtattt cttttgtatt tgcaaatatt caattagtag atgcaatatc
      421 atctgtagaa aaaatacaag gaaaaaataa gtatgaaata gcaggcaaaa tagcagataa
      481 aaatgcatat aaaactgcta tattaattaa tactagtaac tctattgcag atggattaag
      541 tgcaagtgga ctagcaggag ctttaaatgc ccctatacta ttaactgaaa aaaatactat
      601 accaactgag acatctgcaa gattaaaaaa tgtatcaaaa gtttatataa ttggaggaac
      661 atattctatt agtacatctg tcgaaaattc attaaaatct aagaaaatga aagttgtaag
      721 aattaaggga aatgacagaa taaaaacaag ttataatgtt gcaaaagaaa taaattcaat
      781 aaaaaaagtc aatactgtta tgctaaccaa tgcatataaa ggagaagcag atgctataag
      841 tattgcatct gtagcagcaa gagataaagc accaataatt cttactaatg gacaaagtat
      901 acctttttca actagtggtt taaagtctta tgtaatagga ggaacagctt caatgagtac
      961 cactttagta aacaatacta aatctactag actaggaggg agtactcgtt ttgaaactaa
     1021 taaagcaata ataaataagt tttataagga tgctagagag ttttatatag ctggagctta
     1081 tgaattaaca aatgcacttg taggatcttc tttatcaaaa catggtccaa tagttttagt
     1141 taatgatggt agcaataaaa gcattttaaa aaatgctaag aagataactt ctataggata
     1201 tatagattca aatatagtac aacagtgttt aaatataaca aatggaattg gagatattaa
     1261 tactggggtt gtaaaaaatg ttaaacctac tacaaagact ataaaagatg gaatgtataa
     1321 ggttggaaaa gatatatcag caggtgaata cttaattact tcaaactcag gaagttatgc
     1381 ttcttattat gaagttacaa gtgatagtac aggtaatgct gatagtatac tttcaaatga
     1441 tatttttagt gggactagat atatcacctt aaagaatggt caatatatta aaattgagga
     1501 ttcaacaatg acattagcaa agtatgctaa agcccagaag gccaagaatg gtaagtttgg
     1561 aaatggtatg tataaaattg gattagaaat tcctgctgga gaatatatta taatgtcaaa
     1621 ttcttctgat gcatattatg aagttagaaa tgatagctta ggtaatgcag agggaatagt
     1681 tactaatgat actttttcag gaagaagata tataacggtt gaggaaggtc aatatttaat
     1741 attaaatgat tgttatttaa ttgaaaatga atagatgtaa ttgtatttaa tagtaaatca
     1801 tatattttat ttaaggaaag atgttttaag tt
//
LOCUS       DAEPOG010000307         1700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEPOG010000307 DAEPOG010000000
VERSION     DAEPOG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.311"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            300..1571
                     /gene="serS"
                     /locus_tag="KR432_003643"
     CDS             300..1571
                     /gene="serS"
                     /locus_tag="KR432_003643"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0642147.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          727 a          174 c          302 g          497 t
ORIGIN      
        1 tatataaaag ttaataacta tacaagattg ataaagaaaa agtcaaaata gataattgat
       61 tagcaaaata gatataatta taatgaagtt aaatctagca atattctcct aaaattaaat
      121 ttcatttatt taattaaata taatttgaca atggacaaaa aagtgagtaa aatataaata
      181 aaataaaaat aacaggttcg gataaagaaa aaggtttctt aacctcaagc ataaagtata
      241 ttatctaagt tatttgagat taagagataa atccaatctt aggtactgag gaggaaaaaa
      301 tgcttgatat aaaaagaata agagaaaatt tagattgtat aaaaaaagca atggaaagaa
      361 ggggcgaaaa agactttaac cttgatgaag tagttaagtt agatgatgaa agaagaaaga
      421 ttcttcaaga agttgaagtt atgaaacata aattaaatac agcatctaaa aatataccaa
      481 atcttataaa agaaggtaaa gatgtagaaa atgaaaagat taaactaaaa gagttatctg
      541 ataaaattaa ggtaatagac caaaatctta aagaagtaga agataagatg gaatacctat
      601 taatgagaat accaaatgta ccacatccag aagtaccaca aggtgaaact gatgaagata
      661 atgttgaggt aagaacttgg ggaaagacta ctacctttga ttttgaatct aaggctcatt
      721 gggaaattgg aacagaatta ggtatacttg attttgaaac agcgagtaaa ataactggtt
      781 caagattcac tctttacaaa ggattgggtg ctagattaga gagagcatta ttaaattttt
      841 acttagacac gaatacaaaa gtaaatggat atacagaagt cattccacca tttatggcaa
      901 atagaaatag ttttttaggt acaggacagt tacctaagtt tgaagaagat atgtttaaaa
      961 tagaaggtct ggactatttt atgattccaa catcagaagt tcctttaact aatatacatg
     1021 ctaatgaaat acttaaattt gaacaattac caataaatta tacagcttat actccatgtt
     1081 ttaggtcaga agctggttct gctggaagag atacaagagg tttagtaaga cagcatcagt
     1141 ttaacaaagt agaaatggtt aaaatagttg caccagaaga atcttacaat gagttagaaa
     1201 agcttactaa caatgcggaa actatgctac aattattgaa tctgccttat agagtagtaa
     1261 agatatgtac aggtgattta ggatttacgg cttcttttaa atatgatgta gaagtttgga
     1321 tgccaagtta caataggtat gttgagattt catcttgttc aaactgcgaa gatttccaag
     1381 ctagaagagc aggaataaga tttaaaagag ataaagattc taaagctgaa tatgtacaca
     1441 cattaaatgg ctctgggtta gctattggaa gaagtgttgc agctatatta gaaaattatc
     1501 aacaagaaga tggttctgtg gttgttcctg aagttttaag accatatatg ggagtttctg
     1561 ttattaaata agagttaaat tatgtatttt taagcaatac aataatatag aagtaataaa
     1621 aaataattaa tataaaagtg atacaataat atagaagtaa taaaaaaata gttaatataa
     1681 aagtgataca ataatataga
//
LOCUS       DAEPOG010000308         1692 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEPOG010000308 DAEPOG010000000
VERSION     DAEPOG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.312"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            73..1671
                     /locus_tag="KR432_003644"
     CDS             73..1671
                     /locus_tag="KR432_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0642148.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT          658 a          177 c          291 g          566 t
ORIGIN      
        1 taataagttt acttaaaagg ttgtatataa ataatttcaa atatatatcc taatttatag
       61 gaggttttaa ttttgaaagc tgcaatttat tcaagaaaat caagatttac tggaaaaggt
      121 gattctatag aaaatcaaat acaattatgt attgattatg caaagtctat tggtgtaaaa
      181 gattttttga tatatgaaga tgagggattt agtggtagca atatagatag accacaattt
      241 aagaaaatga tgcaggatgc taaagataag aaatttagtt acctagtttg ttatagatta
      301 gataggatat cgagaaatgt atctgacttc tctactttaa tagaaaaatt aaatgaacta
      361 aacatatcat ttatatctat caaagaacaa tttgatactt caactccaat gggaagagct
      421 atgatgttta tatcaagtgt atttgctcaa ttagaaagag aaacaatagc agaacgtata
      481 aaagacaaca tgtatgaatt agcccgtagt ggtagatggc ttggaggcaa tacacctcat
      541 ggattcaaca gtgaaaaaat cacattctta gatgaaaatc taaaagaaag aagtatgtat
      601 aagcttcaag taaatgatga acaaatggag attgtaaagc taatatacaa taaatattta
      661 gaacttaggt ccttatctaa gttatataaa tatgtctata ataatggaat aaggggtcct
      721 cgtggtggta aatttgaccc tagttcttta tctagaatac ttagaaatcc agcatatgta
      781 aaagctaatg atgaaatttt agattacctt aaaaatagtg gtatggatgt tgtaggtact
      841 ccagatagaa aatgtggaat ccttacctat gctaaaaata catctaactc aatagcagca
      901 attgcaaaac accatggagt catagaacct gaaatatggc ttgaagtaca aaatcaatta
      961 gataaaaatc gtgataaaac tccaaggata tcaactggaa aaactgctct tttatctggt
     1021 cttataaaat gtggtaaatg tggttctaat atgagaatta cctataaagg caaaaaaact
     1081 gacaaggatt taaagtatta ttatatttgt ggtactaaaa aaacacttgg gtctgatagt
     1141 tgcaactgta aaaatcttaa tggtccatta gttgaaagtt tagttataga taaaattaag
     1201 aattgtaaag aagagtctat tataagtgca ttcaatagta ataacattaa gactgataat
     1261 gtttttaata tttttaaatc tgagactaat ttacttaaaa atcaaatttt agataaggaa
     1321 agacttatta aaaatttagt tatagagctt gcaaaaaata ctggtagtac tgcttctgat
     1381 tatatagttt cccagattga gagcctaaat aatgagatta gtattttaaa ggataagatt
     1441 aatattttgg aatctagtaa tatcaatatt agtagtgaca gtattaatat tgatattatt
     1501 gttagtaatt tgaagagatt taatgatgaa gttgataatg ccagtttaga tgataagaga
     1561 ttgttattat ctactattat taatagtatc atttgggata gcagtttagg aaatcttaat
     1621 attgtttata tgggtgctga agtggataat tctgtttctt taagcaatta gttgcacttt
     1681 tttagctata gc
//
LOCUS       DAEPOG010000309         1685 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEPOG010000309 DAEPOG010000000
VERSION     DAEPOG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.313"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..615
                     /locus_tag="KR432_003645"
     CDS             <1..615
                     /locus_tag="KR432_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBH0642149.1"
                     /translation="LFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDICLKLN
                     IKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHEL
                     YSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHI
                     RPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            569..997
                     /gene="ytfJ"
                     /locus_tag="KR432_003646"
     CDS             569..997
                     /gene="ytfJ"
                     /locus_tag="KR432_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBH0642150.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1245..1685
                     /locus_tag="KR432_003647"
     CDS             1245..1685
                     /locus_tag="KR432_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0642151.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
BASE COUNT          727 a          129 c          271 g          558 t
ORIGIN      
        1 ttatttaatt ttaagtattt aataatagct gtaattttta ttataagtgt aactatgttg
       61 attttaatat ctcatataaa catcttaata atagctgata taaacaacaa agatatttgt
      121 ttaaagctta atataaaata catgtttaat ttaataaata taaatagaca actttatcca
      181 gctgaaaata gtaaaaataa tgataaaaaa gaggggatga agaataatat agattcaagt
      241 atattattag ctgatgacct tttgagcatt tatagattat taaaaaagat aaaaatacat
      301 gaactttatt caaatattaa ttttggaact ggaaatattg gcttaacatc ttctgtatat
      361 gtattgataa atactttgta tggaaattta tttaatatga ttgatgcaga aaaaatgtat
      421 ttaaatgtaa atccagattt tacaaaagat tatgttttag gaaatataag gatacatata
      481 aggccaagaa taaaagcttt atttaatatt ataataatga ttaataaaat tatgaataaa
      541 aataaaggaa ataaggaagg tgatagtaat gagagcaaca ggttcgatac aaagtcttat
      601 ggaaacaact cttgaaacaa ttaaaggttc tatagatgca aatactatta taggagaccc
      661 tataaaaaca gatactacag tggttgtacc tatatcaaag gttacaattg gctttggtat
      721 aggaggagga gaatactcaa aaggatatga tgataaagat agggaaattg aattaaaaaa
      781 tgaaaaatca gatacgaatt ttgctggagg tagcgcaggg gctatatctg tacaaccagt
      841 agcatttgta gtagttgaaa gtggagagac tagaataatg agtttagaca gtaatatcaa
      901 tctagtggat aatatattat ctataactcc tagagtattg gagaaaattc aaaatatatc
      961 acaaagtaat aagaatcaag ataaaaacaa tatgtaagaa taaaatattt ttaaatatgt
     1021 atatagtttt aagtaaagta gtggggttag tataaatccc actattttgt tgtgtacttt
     1081 aaacatttat atctaataag attttagttt aaaacaattt aaatagataa taaagttgat
     1141 atatagtata attataaaaa gaaaagaagt attatattat agatatataa acgcatttga
     1201 gatataagag ggagttataa atcattagtt ttgaggtgaa tgttatgagc gataaagaat
     1261 tatctatttt gttgttagaa agtatgaata attttcataa tttaataaag attattaata
     1321 acgaaagata taaaaaagat aaggttctta cagaaagaca gttctttgcc ttagtaaaaa
     1381 taagaaaaca tgataaaata gagcttaaaa atctaagtag agatttacat gtatctactt
     1441 ctagtctgtg tatactactt aataaattgg ttgagcaaga gtatgtatat agggaagaag
     1501 atagtagaga tagacgaaat actttttatg gaataactaa aaatggagaa aagatattgg
     1561 ataatgaaat actaaagttt gtatctataa taagtgataa aatggattgc ttagatatag
     1621 ataataaaga taaattattt acttctttgg aagagtctaa aaatataata gaacaattat
     1681 tttaa
//
LOCUS       DAEPOG010000310         1639 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEPOG010000310 DAEPOG010000000
VERSION     DAEPOG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.314"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(116..505)
                     /locus_tag="KR432_003648"
     CDS             complement(116..505)
                     /locus_tag="KR432_003648"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH0642152.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(517..977)
                     /locus_tag="KR432_003649"
                     /pseudo
     CDS             complement(517..977)
                     /locus_tag="KR432_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1406..>1639
                     /locus_tag="KR432_003650"
     CDS             1406..>1639
                     /locus_tag="KR432_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642153.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINY"
BASE COUNT          576 a          255 c          185 g          623 t
ORIGIN      
        1 tctattgttt tgactaattg ttcgtatgta tcaagttttc ttaaatagta catttccact
       61 tttaaaattc tccaaaatcc ttccatagga gcattatcaa tacaacgagc aactcttaca
      121 tactttgtat catgccttgt gttaaaagtt ttgatttaaa taccttacta gtatactgga
      181 aacttttatc attatgaaaa ataggttttg catttggata tagctctatt gctttatcaa
      241 aagtttctaa caccaagtta ttgttatttg actttccaat aacatatgaa actatacttc
      301 tatcgtctaa atctagaatt gcgcttaaat aggccttcat cccattggtt aatttaaatt
      361 cagttacacc tgttaaccat ttttgagagg ttttatctgc actaaatttt ctacttaata
      421 cattttccgc gatcacttct ggcgtacatt taacataact aaatttcttt tttctgcaaa
      481 cagaacttaa gcctacggct ctcattagtc tgtacactct ctacaatctt taacaatttc
      541 aactctttca tctaatgctg tttttctagt ctccataaag atttctccct tcgaggtttc
      601 ttttatctac ttatgaggat tatactcttt accccaagct tttagtgaat aaactgatct
      661 cattttatat ttttttgaga ttgtgtcata agatccttca ccatttagat aagattttat
      721 agcattcaat ttaaattctt cacaatagca taatttacaa ctttgtttta ttaaaccatc
      781 atttccaaaa gcttcataat ttcgcatcca ctctctgaat gtacgtttat ccaatattta
      841 attcttccat tatttttatt actgtattct caccttttaa atatgattca attgcttcga
      901 tttttttgct ctgaagatat cttagtttta gatagtctta catattttaa ctgtctccta
      961 agatgagtat aattcattca tcatcagttc tatagaatac acctagttcc tacttactgt
     1021 aggaactagg actatatgaa acccattcat agattgtagt ttcttctgtt gcattatagc
     1081 gcagaatagt aaattcatgt gaaattttat tttgaattta ctatttatat ataaatgaag
     1141 agtggtctta ttttcaatta aaatttggaa caattttggg ttgacatcta caattaagga
     1201 tttaagtttt tgttatacta ataaataaaa acagagattc aaaatatata aataatactt
     1261 aaactagtgc tgatagattt tctttaagtt ctagtttttt tattgtcatt atctaatatg
     1321 tactatttta tgtacagtca atataatctg aaattgtaaa atggtgtata atatagttat
     1381 aagtaaagaa gggatttaaa catgtatgat tacaaaaata aaaaagactt atatatacct
     1441 taaggaaata caaaaaatat acaaaatcaa tgagcataaa gaactagtac acataataaa
     1501 aaaactaata aatgataata aaataaaacc aataaaaacc tcagatctaa cacctcagca
     1561 tgagcaatta tatacaaagt atagaataat aaaacaagaa acagaagaag ataaaaaaat
     1621 attagaagaa ataaattac
//
LOCUS       DAEPOG010000311         1638 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEPOG010000311 DAEPOG010000000
VERSION     DAEPOG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.315"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            24..491
                     /locus_tag="KR432_003651"
     CDS             24..491
                     /locus_tag="KR432_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0642154.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          682 a           81 c          239 g          636 t
ORIGIN      
        1 tatgtgtata ttaaggaggt aatatgaagt ttttaaatgt tttaatagta gttgaagata
       61 ttgagaaatc aaaaaagttt tattatgata ttttggggtt aaaggtaatc tgtgattttg
      121 gagaaaatgt tgtattagaa ggtaatatat cattacaaga aaaaaagctt tggttagaat
      181 ttattaataa gagtgacagt gaagttaagt tcaatggaaa tgatgctgag ttatattttg
      241 aggaagataa ttttgatact tttgttgaaa gattgagtac aatgaaaggt atagattatg
      301 ttcatctagc tatagagcat aggtggggtc aaagagcaat tagattttat gatttagatg
      361 ggcatattat tgagattgga gaaactatgt catctgtgtg tagacgtttt ttagatagtg
      421 gtctttcaat agatgaggta gctaaaagaa tggatgtaac tgtcgaatat attgaatcag
      481 ttttagaata gatggattat attaaagtaa aactatattt tgtatagaag taagggctgt
      541 aagtaaactt gtatggaaac aaatttactt acagtttttg tttttgtaag atacagaata
      601 taatatttgt tataatttta tttggatttt taaaaagtgc atgattttcc ttacatatta
      661 taatagaatt taatatttat tggatgattt aaatttagaa aaatataagt attagcttat
      721 aaaagataat tatacttact atcaagctgt agaatcatat atactatttt atgtaattat
      781 gttaaaattt aattgaacaa attaaaaata tagtgaaaat tgtagtttta gtttatttat
      841 ttaataaata tttcaaataa aataatgttt aaataagaaa cataaataat aatattatta
      901 ttgttcttaa agacaaaaaa tcttagctta aaaatttgta tcatataaaa tccctacata
      961 ttatggaagt aatgatacat tatatgatat acacaataaa gtataaaagt atttacatgt
     1021 tttttctata tattttataa gttttaggta tggaaaatta ctaaataaaa attagattaa
     1081 tgattatgat agtatagata aagtttattc tttttattaa tagaaattgt tgtataaaaa
     1141 taataaaagt gaggagagtg atatttaaat aaaaaaattt taataagtat tggtaatata
     1201 gtttttaggg atgattattt tatgtaaaaa gttgttattt gtaaagataa atatagaaaa
     1261 tattataagt attgatttag tgataatcta aaaggtgaga attgtaataa aaagagaaca
     1321 tatccttcaa atggatgtaa acttataaat aacattatta caagagattt attatacata
     1381 tactgcaact attaattaga atttatactg tatttgtatt taaataaaaa cacaaataat
     1441 attattgtaa aaatttataa ttattaaata tttaaagtat aagatataat aattataagt
     1501 aatttatgta taaatgtata aaatatagat atatttagat taaataataa ttattattat
     1561 acaaaaattt tgtattaaaa aatatcttta aatttgaaat ataataaatg ttagatagaa
     1621 ttttataaaa tttaaaat
//
LOCUS       DAEPOG010000312         1609 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEPOG010000312 DAEPOG010000000
VERSION     DAEPOG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.316"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            245..1585
                     /locus_tag="KR432_003652"
     CDS             245..1585
                     /locus_tag="KR432_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0642155.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          631 a          126 c          252 g          600 t
ORIGIN      
        1 aaactttata aataatatat tttataccta gttatagata actttatata gttattatta
       61 aaaaaagcaa taaaaagttt aattcaattt agcaaattta gtgatataat taaaaaagaa
      121 aagaaatatg gacttatttg gaataatttt gacaatctat ttactacacg tgtagagtcg
      181 tatataatta aattatacat aaaatataaa aaaataattt agtaatacgt taggagaaag
      241 tattatgata aagtcaagct tattttggaa tattttcatt gcgatatcct ttatgataga
      301 atggtttctt tgtaagaaaa tgctagatta tacaagtgag aaaaggattt ctaacaaaaa
      361 aataaacttt tgtatggtag gtattattgt ttttgctgac ataatatatt tttttgatgt
      421 acctccaaat ataagagtaa ttatatctat gataattact attgcatttt ataaaatctc
      481 atatgatgtg ggatttataa agtctgtatt aatatcctta atttattgga tgttactaat
      541 cgccacagat gctttgagta tatctttaac agtttggctt aattctataa atgacatgag
      601 tagtatgggg acgggagata tatacagaat tgagtctata atattgggaa agatactttt
      661 gattgtgtct ttgtttttat atagagcctt aaagataaaa cttaatatag aaataaataa
      721 aaggacatta ttttatatag ttattcctat agtcgctaat atagcatctt tttttgtaat
      781 ttttgaatac atatttaatc cagggtctgg tagcttaata cataatttcc aattcgttat
      841 gatttctgtt gtattgctta tatcaagtat cactttagtt ttaattgcta aaaaattaca
      901 gcaatataat actttagttg tagaaaacaa tatgattaaa aatactttga agtatgaaaa
      961 tgagtattat gaagatatag aaaagaaata tttaaaaact agattactaa gtcatgatat
     1021 gaaaaatcac atactgtgta taaatgctat gattaaaaaa ggtattgatg ttacttctta
     1081 tttaaataat ttgcaggtag aaattcagag taatgacttg ttatttaata ctggtaattt
     1141 tatattagat gctatattaa gtgaaaagaa agaagtttgt gataagaatg atatagaact
     1201 aaaaatagga ataaatttta gttgttgtga agctgttgaa ttagtagata tatgtcatat
     1261 attttcaaat attttagaca atgcaattga ggcatgtaat aaaattgata gtggaaatag
     1321 agttatttgt gttaaaggtg atgttatgca aaatttttat ttaataagag ttgaaaatac
     1381 taagatgaat aagattatta ctaaaaatgg agatattttg acagacaaaa aaggttctct
     1441 tcatggactt ggaataaaaa gtgtaaaaaa ctctgtaaat aagtatggtg gtaatgttgt
     1501 aattgaatat gatgataaaa aatttgtagt taaaattgct attccactta gaaatgtgaa
     1561 gaaagcagtt aatcatgtga tttaaatgta tataaatttg atgtgtaga
//
LOCUS       DAEPOG010000313         1598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEPOG010000313 DAEPOG010000000
VERSION     DAEPOG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.317"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            37..768
                     /locus_tag="KR432_003653"
     CDS             37..768
                     /locus_tag="KR432_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642156.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            1010..>1598
                     /locus_tag="KR432_003654"
     CDS             1010..>1598
                     /locus_tag="KR432_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBH0642157.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT          693 a          152 c          241 g          512 t
ORIGIN      
        1 tcacaaataa aatacttatt aaaaaggaga tttgatatgt taaacaaaaa aaatgtaagt
       61 ttaattgttt tagtaatagt tttaagttta ttcactgcta tatttgtttt caataataga
      121 aatacaaatg ataaatcaaa agaaattgct aaaaataatg aagaaatagt gcctaaaaac
      181 ttagaagctt taagtataga acaattaaca aaaattagag ataaggctat tgataaaaat
      241 ttagaaggtc ttagagaatt tggagaagat tcagtaaaac agcgtaaaga ttcaatttta
      301 aattttgaat ttggattagt tgcttttgtt aacaaaaatt atagtaactt aacagaaaaa
      361 gagaaattaa taagacaaat taatattgtt aactatgaaa actctataaa tgacataatg
      421 gatccttcag gttcattctt tgaattagca aatcaaagtt atgaaattaa tgagttcatg
      481 ttaaaaaaca aaaaaaatta taaagaatgg tcatatgaat atatagaatt cttaattgat
      541 catttagatg aattatgcaa atttgttgat tttgatataa aagatgtaat agatattatt
      601 gatcctacaa taaaaacaga tctttcagaa gaacaaaaga aatctttgaa tgataaatta
      661 aagaaagtgt cttcaagtga tactttaagt gaagacatta agaaggagat aaaaaattgg
      721 gaaaacaatt tgaatagttt aaatatgaat aaaaataatt ctaattaact gatagtgtaa
      781 aatgtacata attataaata aaatagggat gtcttaaaat tgattatttt ctaatctaaa
      841 tattttaggc ctatctaaaa acataagatt atggtctaaa atttgaaatt ttagaccatg
      901 ctttatcttt acaataaatg ggtctttaaa ataaagtgtg tattcttcta aaaagtggtg
      961 ataattaaaa tattaaatat cactatataa aggaaggtgc acacttttta tgtttaacaa
     1021 aaacgaaata ttaaaaaaac ttattgaaga atatgatgtt aaaacaacta cagatattca
     1081 agatatgcta aaagatttat ttgcgagtac aattcatcaa atgttagaag cggagcttga
     1141 cgaccattta ggttatgata gatatggcaa taagaataaa aatactaaaa actctagaaa
     1201 tggatataga aataaaaatg ttaaatcaga ttttggagaa ataactttaa atattccaag
     1261 agaccgaaat ggagattttg aaccaaaggt aattagtaat tataaaaatg atatatcagg
     1321 aatagagaac caagtgattg gaatgtactc aaaaggcatg tctactcgag atatatcaag
     1381 ccaccttaag agtatttacg gaattgatat atctcattct ctagtagcta aaattactga
     1441 tagagttcta cctttagctc atgaatggca aaacagacct cttgatatgg tttatccaat
     1501 tgtatttatg gatgctatac attataaagt tagaagtgaa ggtagaataa taaacaaagc
     1561 tgcatatata gccataggtg ttaacttaga aggtataa
//
LOCUS       DAEPOG010000314         1553 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEPOG010000314 DAEPOG010000000
VERSION     DAEPOG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.318"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..259)
                     /locus_tag="KR432_003655"
     CDS             complement(<1..259)
                     /locus_tag="KR432_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH0642158.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVL"
     gene            complement(282..1538)
                     /locus_tag="KR432_003656"
     CDS             complement(282..1538)
                     /locus_tag="KR432_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0642159.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
BASE COUNT          540 a          262 c          141 g          610 t
ORIGIN      
        1 tcaatacagt taaacttgaa acatctccat ttaatatttg gctcttacta aaattaagat
       61 gcaataaaac ttcttcaata tcccaatttt tatttacttc aaaaaaagta ctattacttc
      121 ctataacacc atcaatagtt atatctcttt caaacttata atttttaact ttagtctcat
      181 cagcatttac tttgtatata agtgatatat ttgaaaaaaa caatactaaa gatataattg
      241 atataataaa ttttttcatg ttaattcctc ctgtacatga atcaaaatct ttctgtttta
      301 taccatttaa cctctctttt aaaaattata tcttttagat actctatcat cccttttata
      361 gctacaaaca gccatagttg agagtatgta aaatacatta ttgcaactat gaatatattt
      421 tcaattgttg cttcaccttt ttctatagta aggcttatac tcacttctat aataaataaa
      481 agataagata gtatccatat tagaaaaaag ttaattggaa tagaaatttc tactagttta
      541 gatatactta aaacacataa aatatcggat ataattacag ttgttaaaaa tagaaaatat
      601 acagaaaaaa agtaagctat atcaaacaca atcttatttc ttccctgttt aaatatattt
      661 tttatatatt tcattaatac atatatattc ccttttgccc atctagtacg ttgctttatc
      721 cacaccttaa cagtttctgg ctcttgttcc catgttatag attgtggtac aagctttatc
      781 ttgtatccca atttatatat cctaaaactt atttctgtat cttctgcaat tgctttacta
      841 tcccatcctt caatttcttc tatgatactt ctcctcaata taaagttagt tcctggtatt
      901 gtacatagat taaaaagttg ccatctacca gcttgtgaca tccattgaaa gcttaatgtt
      961 tctatgttta taaatttggt taataggttt ttatttttat ttcgagtcct aaatttccca
     1021 ataacagcac ctaattcatc attcattact atagtttgta ctagatatct aagtgcattt
     1081 ttatctggtg tgttatctgc atcataaaca gctataaaat ctccttttga aaccttatat
     1141 ccaatattta atgcattaga ctttcctttt ccacctgtta aactatctgt atttattata
     1201 gtgaaattat aattgttgta cctatccttt atattttcta aaatttcttt actattatca
     1261 gaagaattgt catttataac aataagttcc atcttactct taggataatt gagtaataac
     1321 aaagactcca cagttctacc tatcacttta gcttcattat gagcaggaac aagtattgat
     1381 atcataggat attcatcaat ttcttttatt tctttatcaa agtttttaaa ataaaatata
     1441 tatccaccca tagctaaaat aatatttata agcaataaag accatatact aaacagacta
     1501 aatacgaaca aatactctga tacatttaaa tctctcataa atttctccta aaa
//
LOCUS       DAEPOG010000315         1552 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEPOG010000315 DAEPOG010000000
VERSION     DAEPOG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.319"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..477
                     /locus_tag="KR432_003657"
     CDS             <1..477
                     /locus_tag="KR432_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBH0642160.1"
                     /translation="ALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLLAFLDKGYI
                     VGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLVIASLWPTV
                     INSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKNVINIKF"
     gene            534..1520
                     /locus_tag="KR432_003658"
     CDS             534..1520
                     /locus_tag="KR432_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0642161.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
BASE COUNT          603 a          132 c          275 g          542 t
ORIGIN      
        1 gcactaactg cagcaattac agcaatctat gcagttttaa cattatcatt gggatttata
       61 agttatggac caatacaatt tagggtagca gagattatga tgcttttggc atttttagat
      121 aaaggatata tagtaggtct tacattaggt tgttttttag caaatgtaat aggaccatat
      181 ggtgtacctg atataatttc tggaacatta gccacattta taagtgcaag tatgatttat
      241 attactagaa agttaggtaa acaaaataaa agtactttag ttatagcttc tttatggcca
      301 actgttataa attcgattgt ggttggtcta atgttaaatg tattctttgg gcttccatta
      361 attctatcta tgatacaggt aggttttgga gaatttgtag tagtaacaat tgtaggagta
      421 ccatttttct gctttttaat gaaaaaatat aaaaatgtca ttaatattaa attttaaata
      481 gtgtatagta atttaattaa ttgagagtat tattagtgaa aagaggtgtt tatttgtcga
      541 aggtaaaaag attgactgat gtttggatat atattaatga taagcaaaag tttacaataa
      601 aagagttgtc agaggaattt aatctttcag ctaaaacaat acaacgatat ttaaaagaat
      661 taaataaaat gggacttcca attcaagcag aacaaggtag aaatggaggt tatagggtgc
      721 taaataatag ctatatacct cctgttatat ttacagaaaa agaagttatg attatactat
      781 ttgcacttaa gtcacttcag atttataatt ataagttaat ggaaatagaa attaattcta
      841 taatgagaaa agtaagttat gaacgtaaaa gcagtataaa agtaggtata gaaaatatta
      901 aaagatacat tggctttgta gctaatgata ctaatgatgg agattttaag agtagtaatg
      961 taactgagtt ttttagagct tcttcagaat ctctaatttt aaatattaag tataagtgtg
     1021 gtaatcagat tttggaaaaa aacgtatgcc ctataggtat atatttaaat aagggagcat
     1081 ggttttcacc agcatatgat tgtaaaagtg ataaaataat attagttcat atagataata
     1141 taattgatat aaaaagaaca ggagaaagta aaaaaataca tataaacttg gaagaatggt
     1201 tacatgaagt atataacaat ttatatggaa atagttcaac tactaaaaaa tttggtgaaa
     1261 ttatttatct aaatgtatta ttaactaaag aaggtgtttc taaaataaag gacagttctt
     1321 ggaatttaga tatggagcta aatgaagatg gtagtggaat aattagtaca ttcataaagg
     1381 ttgaagatat agattgttat gcttcgattt tattttttat tggaaataat gctaaggtta
     1441 tagagccaaa atttttgaat aagctacttt atgataaggc caatgagtta agatattttt
     1501 atgaagaaaa tatggtataa tatttagaaa tgttgaaatt aaataattat ta
//
LOCUS       DAEPOG010000316         1517 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEPOG010000316 DAEPOG010000000
VERSION     DAEPOG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.320"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..874)
                     /locus_tag="KR432_003659"
     CDS             complement(<1..874)
                     /locus_tag="KR432_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0642162.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMD"
     gene            complement(886..1395)
                     /locus_tag="KR432_003660"
     CDS             complement(886..1395)
                     /locus_tag="KR432_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBH0642163.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
BASE COUNT          503 a          245 c          149 g          620 t
ORIGIN      
        1 tatccattgt atttgaattg ctcatattat taaataataa cgaggaagga acaaatggaa
       61 cttttatatt attttcgtca aatttatttt tgactttttc cattacatca aagtaaacat
      121 cccagtaatc atcagtttta acccatggtc tagcaactat ttgcatagtg ttgtttccta
      181 tagagtttaa acctataatt ggttttgggt catccaatat ccttttttca tttataaaaa
      241 tttcattcaa tatggactta actacatcaa tatcactatc atatgaaata ttatatgaaa
      301 aatctatcct tcttttattt tgatgtgtgt aattaattat attgttactt gtcaattgaa
      361 agtttggtat aattatggtt ttattatctg gggttttaag aacagtacta aatatttgta
      421 tatcagaaac tgtgccttct acacctgagc cttgaatata atcacctata ttaaatggct
      481 taaaaaataa aataatcata cctgaaccta aatttgataa tatttcttta aatgctaaac
      541 ctatactaaa tccagcagct cctaatacag ctacaactga tgtagtttgt atccctataa
      601 cacccaagct tatcaatgat ataatagata acatagatat gtatattcca tatacaatga
      661 aattatttac acctttactc attccatatt tgattaaaaa ccttgaaacc atctttctta
      721 ctatcttaga tatatagata cctattacaa atacgattat tgcatatata atatttggta
      781 tattatcact aaagttatta agcatatttt ctaaagttct tttagttata ttactatttg
      841 ttgaagcagc cttagctaat gataaaaaat tcatttaact cctccttatg ataatattcc
      901 tctgtcctct aattcttttg ttataacttc tatagtctct tcttttaatg tctcaattgt
      961 atgaatatga ctacattcag ttagaactga taactgttta aattcatttg tctcaacctt
     1021 cttcataaaa ttatctatat ccattttaga gtgaatatta agctctgcag ttatctgacc
     1081 atatgttgga tgatttacga tgacatcctt aacaattcca cctaaatcta caataatctt
     1141 taattcatca taaatttctt tagaattcct gtgattctta cattttattt gctttgtaaa
     1201 atatggttga ttatatatta tatatccttg aggtgtagct attatttcaa atcctctagc
     1261 tcttataagg gctatatctt gtactattac ttgcctactt acatttaata tctcagctag
     1321 cattccacct tttacagggc acttagattc ttttaaaata tctattaatt tgcttcttct
     1381 ttcatcagaa ttcatattac accttctttc taaatcaaaa actttaccta tactatataa
     1441 ttctatattt tatcttatta taaatcaata ttgattttat attctattta atattaaact
     1501 ttattgcaaa taaaaaa
//
LOCUS       DAEPOG010000317         1477 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEPOG010000317 DAEPOG010000000
VERSION     DAEPOG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.321"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            89..1327
                     /locus_tag="KR432_003661"
     CDS             89..1327
                     /locus_tag="KR432_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0642164.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          626 a          135 c          252 g          464 t
ORIGIN      
        1 atatatttat atttaaacta ttttgttatt tatataaaaa tacttatcta gttactagca
       61 attattagta gaaatggggg aaatagagat gaataaggga ttttacaaat taaattcagt
      121 ggtattgaac tttgatatta agaatgatgt taacaagata agatttaatc attatataat
      181 gagaaaagaa aacatacaaa gacttcaagg aactctgcca aacggacaat tttatatgac
      241 agtgagaaga gctgctacag atttacaact ttctatttct actataagta ggcttgtaaa
      301 agagtttttg gatttaggga taataagatt agtgtctaag ggtgtaaaag gtaatcgttc
      361 tgtatatagt tacgtaagta tagagtttaa tggaagttta tttgatgata gcaaatatat
      421 ggaacttgac tgtgtatgta atgatgaatt tattgataat agtggcatag agtttgattg
      481 tgatagcaaa gataattctt ttaatggaga tataaatgta gaaactgtgc attgcattaa
      541 tgagtatttc tctgatgatg atgtagagca taattataac aatttccata ataaagatac
      601 tataaaggat aaagccattt ctaatattag cactatcaaa aaatatattg atgaatcttt
      661 tgatacaaat aaatcagaaa attctaactt acaacaggag tcaataaatt tcaactttaa
      721 tgatacatgc aatgaaatag ataaaagaac gaataatagt atagaaaata atcaaaataa
      781 aaggcctatg agattgagtg acatcagtgt tttaagtgat aaaggtatga caaataaaaa
      841 gaattacttt aatcataatc atggtataca aaaaaggaat aaatctgcaa caaagaaaaa
      901 agaatcctta aataaattta ataaaaaaaa tatacagcaa agcattataa atatgctaaa
      961 taaaaaatca ggtaagaatt ttaaaatcga ttctccaata agcataaaac ttatcaatga
     1021 aaggctaaag gagggctatg tcttagaaga tttctataaa gttattgagg taaaagtggc
     1081 taagtggaaa aataccataa tggaaatgta tataagacca gaaaccttgt ttagctataa
     1141 gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg ttcatccatg
     1201 taaagaaggc aatagtaatg atagcagtaa ttatatacat tctgattata caacatatga
     1261 tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga tactggaatc ctatatgtga
     1321 aatttaatta gtttaagaat gtatttatga taaataagat attaatagag aatagtgagc
     1381 tatagcaaat gatatatcaa tagttggtag taagttatat taagcaagat attactatca
     1441 ggtagtgaat tatagtgaac gatatatcaa taattgg
//
LOCUS       DAEPOG010000318         1453 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEPOG010000318 DAEPOG010000000
VERSION     DAEPOG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.322"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(488..676)
                     /locus_tag="KR432_003662"
     CDS             complement(488..676)
                     /locus_tag="KR432_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0642165.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            838..1188
                     /locus_tag="KR432_003663"
     CDS             838..1188
                     /locus_tag="KR432_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBH0642166.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1190..1390
                     /locus_tag="KR432_003664"
     CDS             1190..1390
                     /locus_tag="KR432_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0642167.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT          498 a          184 c          173 g          598 t
ORIGIN      
        1 tataaattct agcatatcat ttgttatttt ctgatatgct attgatgttt taaatcttct
       61 atttatttta ttatttttta cttttccaat tgtaccttta taggagttat atttatgtga
      121 tttatgaaaa aatgctatgc atttaagttc taattttttg ataatacatc taactttctt
      181 ttgattaata atgataccac tatttttaat gtggcttccc tgaggcacac atctttttta
      241 aaaactcaag ctcatctttt aaaaatttat tttcatattc taattcttgt tctcttgtta
      301 atttattttt tctttagtgt ttttgttttt attcattatg gtcttccttt cattttgaaa
      361 aaggcatcaa caccactatg taaaaatttt tgttttcatg ttgctataac tgtgctattg
      421 tttattccaa aatacttagc gatttattgt acactatact tagttatcat catatagttt
      481 ataacatcta acttgaacca tatagtatag aaaacttttt tatttttagt gcaccctctc
      541 ctaatatatt aacccatctt tcaacttgag aatggtcata aactccatat tgttttacta
      601 ttaaaatatc tcttccttct ctattcaaat aaaatttagc tacttttaat ttgaaattaa
      661 aatcatattt agtcatacat aaacatctca agcgttagat ttttactcta acaattatgg
      721 tgcagtacaa atattgtata atactgattt tctattgaca aaagaatata aaagagatat
      781 atttgtatta taaagataca aatatatctc ttttaaaatt actaaggagg ataaaaaatg
      841 attcaaaaaa aaatttttat gggtttttgt ggtttccttg gatttttaag tcttagatat
      901 ttttcatctg gcaatgttac tgatcttaca tatattggat tttttgcttt tttctcaaac
      961 tttattatag caaaaataaa tggtgataaa gcagatgaaa ggtatgtaca agatgaaaaa
     1021 gcagctatgg catttacagg acagcttgct attatagaat tattcatttt atggtgtatt
     1081 actatagtgt ctaggaatgt agaattaatg tgtgttttac tatcaattac atatgcaatc
     1141 actttaaatg tttatgctat aaaactttat attttagagg aaaaataata tgcccaaatt
     1201 catttgtaac ctaaaaaaat atagacaatt aaaagaatta actcaagaac aattagcaga
     1261 aaaagtagga gttagaagag aaaccattat gcgtttagaa gcaggaaagt ataatccttc
     1321 tttgaaatta gcaatagata tatcaaaaat agtagataca cctattgatg aattgtttat
     1381 ttttgattag tttttgcatt attaaaaatg tgttttaaat acaaacttaa attcaatctt
     1441 taagtcactc ctg
//
LOCUS       DAEPOG010000319         1321 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEPOG010000319 DAEPOG010000000
VERSION     DAEPOG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.323"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..140)
                     /locus_tag="KR432_003665"
     CDS             complement(<1..140)
                     /locus_tag="KR432_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642168.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIV"
     gene            complement(653..>1321)
                     /locus_tag="KR432_003666"
     CDS             complement(653..>1321)
                     /locus_tag="KR432_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0642169.1"
                     /translation="KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGG
                     SDTGTTGKPLGGIKEKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNS
                     LRPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAK
                     TRTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIE
                     DSLK"
BASE COUNT          435 a          219 c          140 g          527 t
ORIGIN      
        1 acgatacttt ttttctctaa gtttatattt aaatcagcgt tattttccat agcattagat
       61 tcagtagcaa atgccattgt aggtgcgacg attgccattg aaagaatccc tgttgttaat
      121 aattttgtat ttcttctcat attaatctcc ttacttaaca taaatttcta ctttattttt
      181 aatcttttaa cttttgaaaa tatgtcaaag tgtatctaac ccaaaatata aataatatat
      241 aagtaatata taaaaataaa cttataaaac tcaaaaaata aaaaaattaa atatatttaa
      301 tacatttaat tatctatact atatcattta ttgacataaa aatccacaaa aaacacacaa
      361 acacacaaac acacaataaa atatagtaat taagctttta ttgtgtatta caaatgaata
      421 ctccaactct tgacaattaa ctgtagcctt aagatatgta aattcttcat ataatataaa
      481 tccttttaca attaatcata aaaagactgt atatacctca tttcgcactt atatgcacaa
      541 tgtatataca gtctttatta ctattataat tacattctta tcttatagct ttagtttaaa
      601 tttaaaattg cagattaatt tattttaaat ttttctatgc tctcattgta gactatttta
      661 atgaatcttc tatgccttta cctatttgtg tacccatttc ttttacttta tttgatgtat
      721 taagtaaact catatctttc tcattatcca aaaatgcaca ttctacaagt acagctggca
      781 tatcattatt tcttaaaaca tataaataat cttttgtagg gtcagttgat agtgttcttg
      841 tttttgcacc tctatttttt agattaaatt tctctaagat tctatttaat atatttgtag
      901 ctacagtttt agtagtccct ccatctttat ctttaagctt ataaaatact tctacaccat
      961 tcccagttgt atcagatgca ttatagtgta tactcgtaaa taaatctggt ctcaaagagt
     1021 tagataaagc agttctattt ccaagagata aagtcttatc tgtgtctctt gtcattatta
     1081 cattgaatcc ctttgaacgt aaatattcag ttgtcgcaag tgaagtattt aatgtatagt
     1141 ctttttcttt aataccccct aatggtttac ctgttgttcc agtgtccgaa ccaccatgtc
     1201 ctgggtcaat cataactgtc ttacccccac tatttcctgg ttctgttgga gttgtattgt
     1261 gtttagataa tgatgatgct atagagttca ttaccttaga agtcaatcct cctcctattt
     1321 t
//
LOCUS       DAEPOG010000320         1320 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEPOG010000320 DAEPOG010000000
VERSION     DAEPOG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.324"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(308..565)
                     /locus_tag="KR432_003667"
     CDS             complement(308..565)
                     /locus_tag="KR432_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0642170.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1145..>1320
                     /locus_tag="KR432_003668"
     CDS             1145..>1320
                     /locus_tag="KR432_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBH0642171.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENP"
BASE COUNT          481 a          180 c          126 g          533 t
ORIGIN      
        1 atattcttaa ttttataaat atattatgtc atattaattg ctatttcttt atttatatag
       61 tttatttatt tatttttccc ctttatacaa gcaattgtat cattttatct attttttagc
      121 caacaaatat tttcgaccat tttctttgta ttctcaatat atctccagta tttaacaaat
      181 ttttttatag aatatacaat gtatttaaat taataagtag taaatttagt taatttgtct
      241 aaaaaaactt tataaaacat cttctttgtc tttactctta catttaacaa ggctattttc
      301 attatgttta aacattattt tcattgagta ttcgcttaat attgcttaca cacatattat
      361 atctaatagc caattcatta tggttgactc cattaaattg ttttactata tccctatttc
      421 tagcatatct aaaaagcccc ttatacgtag gtatataagt atttgttcct ccatacaact
      481 tggcaacctc tatgaactta tcaattccga ttatttctac cattgcatga aattgaactg
      541 gtatatcctc cacttttatc ttcataagtt acacctcctt taagatattt ttatagactt
      601 aacaaagttt ttatttaata cattattgac taaactatat ttattagttt tattgaatct
      661 aataaaaaat ttcttagctt taaaattaat ttttgtatta actttaataa aatttctcat
      721 aacttataac tttgttagct ttaataaaga ttttaatggt ttaaaagtaa aatgttaata
      781 ttggactttt aactttttta tttacagatt aaatttaata aaatttttaa cataagcttt
      841 aacatttaat ttaacataaa ctttggtacc taacatttaa ctctacacat atgaaaatcc
      901 atacaaaact atctcaacta agcatgaata tatacaaata ctatttcaaa acactaagaa
      961 catatcttca aaaataactt gtaaactttt tcatcatcag aaaagtaaaa tttttcctat
     1021 aaaaatttac acaaaaaatc tttagaaatt actgtttaac tccgatatta tcttagatga
     1081 tagtattata gctaacaaag aaagtcgaca aatctttgaa attaaatttt taggaggtaa
     1141 ttttatggaa gttgtttcaa caaaaaattt atctagtata aaactaaaat tggatgctgg
     1201 ttttgatgat aagggtaagg cagttgttaa aagtaaatca tttgcaaatg taaaagcaga
     1261 ggcattaaat gaagatgttt atgctgttgc agaagcaata gcaagtcttc aagagaaccc
//
LOCUS       DAEPOG010000321         1319 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEPOG010000321 DAEPOG010000000
VERSION     DAEPOG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.325"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            63..1277
                     /gene="dpaL"
                     /locus_tag="KR432_003669"
     CDS             63..1277
                     /gene="dpaL"
                     /locus_tag="KR432_003669"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0642172.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
BASE COUNT          533 a          157 c          282 g          347 t
ORIGIN      
        1 taagacacaa agataaaaaa ttaaaatgac accgaaaaat ttgaaagggg aatataaaat
       61 aaatgagaga aataaaatgg aaattaaata acttacctaa atcagaggaa aaagaaaaag
      121 gaattgagtt tttaagagat gaagaaattg caaaggctaa agcttttcat gagagctttc
      181 cacaatatga aaaaactcct ttagttaaat tagataactt agcaaaatca ctaggcgtaa
      241 gcggaatata tgttaaagac gaatcttata gatttggttt aaacgcattt aaagttttag
      301 gtggttctta ctctatgggt agatacttag ctcaaagatt agatacagat atatctgagt
      361 tagggtatga caaattaact tcaaaagaaa taaaagaaaa attaggagaa ataactttct
      421 ttacagccac agatggaaat catggtagag gagttgcttg gacagctaat aaattaggtc
      481 aaaaatctgt tgtattaatg ccaaaaggtt cttctgaatt tagattaaat aaaatcaaag
      541 gagaaggtgc agatgctagt ataacagact taaactatga tgatgcagta agattagcta
      601 atgattatgc agaagctgat gaccatggag taatggttca agatacagca tgggacggat
      661 atgaagaaat accagcatgg ataatgcaag gatatggaac tatggctaaa gaagctatag
      721 aacaattaaa agaatatgga gtagatagac caacacacgt atttgttcaa gcaggagttg
      781 gttcacttgc tggtgcagtt caagggtatg ttgcttcaat atatgatgaa tgcccaataa
      841 cagtagtagt agaggctgat gaggctgatt gttactataa atcagcagaa gctggagatg
      901 gaaaaccaag atttgttgga ggagatatgc caactataat ggcagggctt gcttgtggag
      961 aaccaaacac tataggtttt gaagtgttaa aaaattattc atcagcattc gtttcagcac
     1021 cagactgggt atctgctaaa ggtatgagaa tattaggaaa ccctctaaga ggagatgaga
     1081 aagtaatctc tggagaatct ggagcagtta caactggagc attagtttca atccttgaaa
     1141 gtgaagattt aaaagactta agagaagctt tgaaactaga tgagaactct aaagtattat
     1201 taataagtac agaaggagat actgaccctg ataaatatag agatatagtt tggaatggtg
     1261 aatgccaaag taaataaaag acttaaggaa aataaaaact taacaaataa atgaaaaat
//
LOCUS       DAEPOG010000322         1297 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEPOG010000322 DAEPOG010000000
VERSION     DAEPOG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.326"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            306..800
                     /locus_tag="KR432_003670"
     CDS             306..800
                     /locus_tag="KR432_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0642173.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1197..>1297
                     /locus_tag="KR432_003671"
     CDS             1197..>1297
                     /locus_tag="KR432_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642174.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDNITK"
BASE COUNT          515 a          141 c          175 g          466 t
ORIGIN      
        1 caagttatgg tattggtaag taagtataat taaatagaca ttattttctg ggtcctcttt
       61 ttattttcat ctgcttttaa ataatattat tgacattgat atgactgtag ttttctttat
      121 tttaatccat tgcttatatc ttttctaaca agaaatgtca ttttaaatca tttctataaa
      181 ttgtaaaata atagtatatt tgtcatagaa agtgattttt ttgtctatat atggtaaaat
      241 atagaaagta gaaatcaaca aaataaaaat ttatatatta ttaattttat atggagggga
      301 aataaatgaa aaataaaaaa ggatttactc tagtggaatt attagtagta attgctataa
      361 taggaatatt ggcaataata gcacttccag cattatttaa aaatatagaa aaagcaaaga
      421 tagctaaact tgaagctgat ataagtgcaa taaaaagtgc atctcttagt tactatgctg
      481 atgaatccaa gtatactgat ggaggaatga tatcatgggt aaagaaagat ggaaaaataa
      541 taataaatgg gggttttaaa gatgacccat tagcagataa aatagaaaat ttagggatgc
      601 cttataatgg ttcatatctg ttaatgtcat ctcctggtca tgaaaaatat ctagaattaa
      661 gcatacttcc agaaggagaa ataagcaaaa gtggtctaga taaattaaaa aatgattatg
      721 gaaatttaat agacataacg aacgatcaaa ataaaataaa tattgtaata aaacttttaa
      781 ataataaatc gaatacttaa atacaataaa tttgatttaa taattactga tgaaataaac
      841 aatcttgttt ggagaaatga aaaaagcact ccccaagaat agaatgctta gttataacta
      901 cagaagtgta ttggcataca cttctatttt ttattatatc acataattta tgttgctaaa
      961 aatacctatt taagttgttt ttaaattgtg ttgagtaata ttcttgatag tttatacatt
     1021 aatgaatttc aaaaaaataa gcactcttta taataaaaag tactttagtg tatgttaatg
     1081 tcctatctaa taaaattgta acatgtctta tgctttagta tgataatttt cattctcttt
     1141 attgttgcaa cttatactat agttttgcta atttatctgc aaacacaact tctttattgc
     1201 atgtgtatca accaaaaaca tcagaatact tacttaatac atctgcaaaa tcgtatcttt
     1261 catctatagt attcttattt gacaatataa caaaaaa
//
LOCUS       DAEPOG010000323         1255 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEPOG010000323 DAEPOG010000000
VERSION     DAEPOG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.327"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(281..772)
                     /locus_tag="KR432_003672"
     CDS             complement(281..772)
                     /locus_tag="KR432_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBH0642175.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          491 a          173 c          111 g          480 t
ORIGIN      
        1 tgataagtat ccaaaagaat tactggatat aataagatag ggtatttagc cttttaagat
       61 aaataaaaat atctgcaact atatattgca aaaatttaaa actaatttat atatgctaat
      121 ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta acttatatat
      181 tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt catatctaag
      241 atatgttatg acactaatat tcacaatttt ttagtaatta tcacattata aaaaatatta
      301 cagctagtcc atcatcagat gcttcttgta taaacttttt aagctcaatt agatttgccc
      361 atacatattc aaaaaaatca tcttcactct catcattcat aacaggataa atattatttt
      421 ctaacatctc attaatatta aatctttttc taaatgcttg ttcttccaaa ctgttcattg
      481 cttttgatag tttttttaca tcatttgctg taatcaacat gacaggaaat tctccatcaa
      541 tttccataag tatgtttcca cttagtacta atttgctaaa tatattctca tcatctccac
      601 caaaaggaca acctgtcaaa gtaaagtgaa tggcatgcca tgctttatca atattgatag
      661 tactattttc gttggcatct tcaaaaacaa aatcttgtaa attttcttct gcttgctgta
      721 atttaaaaac attctcttca cttattttag tataacatcc tatcatcccc ataattacat
      781 tcctcctaag tagcgttttt ttaattgtaa cttataatta aacttagtta caataaaata
      841 tattgagaaa agtattattt catcttaaat ttaaatacct atattatcaa ctaaaattct
      901 gtgtttatag cgtaagtatt attatttttt cctaaaaatt aaatgaatca gaagttctat
      961 aggcaatatt gactataaat aagaactatt acttttaaaa aaaatttaag aaattatact
     1021 gtagaatcat gctctactta ttagcttcaa ataaaaaata aatcatgttc tcaaattaaa
     1081 taataaataa caataaattt aaattttaaa ctataagagt ttctgaagtg ttaataagca
     1141 taccaataat tatatgtgta agaatttgat ttaatagata tgaataattt ttacattaat
     1201 tataaatata ttaacctatt attataaatt ctataaaaag aaaagttatc ttaaa
//
LOCUS       DAEPOG010000324         1179 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEPOG010000324 DAEPOG010000000
VERSION     DAEPOG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.328"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(129..839)
                     /locus_tag="KR432_003673"
     CDS             complement(129..839)
                     /locus_tag="KR432_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0642176.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          422 a          155 c          126 g          476 t
ORIGIN      
        1 aattttgaaa gattgaaatt ctagaaatta agcttcaaaa aattaaattt gaaaaaacaa
       61 aactctaaaa actgagcttt aaaaaattaa gtttcaaaaa taaaattcta gagattgaac
      121 ttcaaaaatt aaggttctaa catatacctt gtaaattttc ctacaacctc ttgatatttt
      181 ttacggctta taggaatttt ttcattctga actttggcat aagtttgagt tatactatct
      241 acatggttta gattgacaat atacctttga tgcgtacgta taaaattaga gggaagaaca
      301 gtttccatat catttaattt tccgtagtat tctaatgttt tatcaaaggt aactacagtt
      361 aattttctta gctcgctttt aaagtagagg atatcttttg tattaatttt acaaagatta
      421 gttccaatat gaactgtaat aaagttttct ttccattgtt ctaatttttt tagtgcttct
      481 tttaatactt tacatatttt ttcagtttta taaggtttta agatgtagtt caaagctcct
      541 acatcatacc catgaaatac atagtcagaa aaacctgtga caaagatgat tatagtatct
      601 tttgaatgtt ttcgtatagt ttttgctatg tcaacgccat tacttttttc aagttcaata
      661 tctaaaaaaa taatatctat gtaataattt tcgtgttcta gattgtatac tagactttct
      721 ccatttgaat attcttttat ttggtagggg tagccccaca aatccatttc tcttgtaatg
      781 attttattca gcatgtttct ttgttgtttt tcatcatcac aaatacaaat cgaagtcaat
      841 tttttatctc ctttcaagtt ttatatttat atagttaagt atataatata ttttgctacc
      901 tagatatata aaatgaaata acaattatat ccagatagca aaataaaatt ttttatatta
      961 gtatacttac acttaaaact attctattaa acttattctg aagtgctaat gattttctta
     1021 ttaaaaaatc taataacctt tcttattata aaataccaat gcttttatta ttataaaaat
     1081 gtaatgactt ttcatattat aaaaagcaaa tgattatctt actataaaaa tacaatgact
     1141 tttaatacta taaaaagcaa ataattatct tactataaa
//
LOCUS       DAEPOG010000325         1094 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEPOG010000325 DAEPOG010000000
VERSION     DAEPOG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.329"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(201..416)
                     /locus_tag="KR432_003674"
     CDS             complement(201..416)
                     /locus_tag="KR432_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642177.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(599..943)
                     /locus_tag="KR432_003675"
     CDS             complement(599..943)
                     /locus_tag="KR432_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642178.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
BASE COUNT          363 a          162 c          131 g          438 t
ORIGIN      
        1 ccaaaaatat ttaaaaacat ctcaatatac attttttata atttgttaaa ataaaattca
       61 tatattaaga aatattaata gtaaataagc aattgttact ataacgtagg aattatcttt
      121 aaaaacacaa attaatttac attctctaat tttaaagtat atgtatatct ttgattttaa
      181 taaaattcac ttaaaatatt ttaaggttga acaatgaaat tgaatgccca agaacttcct
      241 tctcttacta atgttgcaat taattcaaac ccacaattgt attgaactga atctctacct
      301 actggtttcc aactagaagg tgcattatat gagaaagaat ttgatgttgg ctctttcttt
      361 tgttgctgct taattcctag cccattagaa ataaatccag tttgatttat atgcaccctt
      421 ctatttgtta cacgaatctg gccgtctgca atagaccagt ttccagaaac tttactaacg
      481 gaaatatatt gaaatgctcc atctcctttt gatattgtat atgtaactgt accagatgct
      541 tttacaccac cagttttatc ccatccatct tctgatatac ttgcttttct aagaggcttt
      601 attgtattag tatcaaaaaa actgtcttag aatatatctt tgatgttaaa tttttagatt
      661 gtttaacttc taataattga gttgttgata tagaatcaac gtatatggac tctcctgttc
      721 ttgcattttt gattatacct ttttcattgg aacttttaac atcagtaatt ccatttttag
      781 ccatatcgta taatttgtcc atgtctgtta cttgtttctc tgaatacatt actttcatag
      841 atgaatcttc taccttacta gcacttgcat ttgttggtaa tagtattgtc attatgagta
      901 aagataaaat agttgttaca gattttttta acctataatt catattaatc cttacttttt
      961 atttgttata acagacattc tattgtaata gaatataaat ccacaatatt tttaaaatta
     1021 gtattgaata actattctca tgttataatt attcaaagga gggattgata tgaggaaaaa
     1081 attttttata ggta
//
LOCUS       DAEPOG010000326         1064 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEPOG010000326 DAEPOG010000000
VERSION     DAEPOG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.331"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(204..404)
                     /locus_tag="KR432_003676"
     CDS             complement(204..404)
                     /locus_tag="KR432_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0642179.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(629..805)
                     /locus_tag="KR432_003677"
     CDS             complement(629..805)
                     /locus_tag="KR432_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642180.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(859..948)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          392 a          158 c           99 g          415 t
ORIGIN      
        1 tactatatat tctaattaaa agagtacttg taggtttaag tacatacttt ataaatatat
       61 ttttgtcaga tttattttat aaaaagtatt tagactaagt attattttta agtattttct
      121 tgttgaagtt attttttcct tttttacaaa atgcgtaaat aaaaaaaaga ctcttaaaag
      181 agtctttccg aaaaatctat atattataat atagttatat tttctgcttg aggacctcta
      241 gcgcctttaa ctatatcaaa gcttactttt tgaccttctt ctaatgattt aaatcctgaa
      301 gtttgtatag ctgagaaatg agcaaatacg tcatctccac tttccattga tataaatcca
      361 aatccttttt cattattaaa ccattttact attccattat tcattaaaaa tactcctatt
      421 aacctatatc aagatatact tgatttactt gtgtattacc acaaatacta ttatatcata
      481 gattaacata aatgtctatt taattataat ttttagttaa catttttatt tattcttaat
      541 atgaaaataa atttaaacat tttatattac caatatatta atgtaaatgt agataaaact
      601 aaaaagggtt ttctagaaga aaatctgtct aattatactt acttaccaat accatcactt
      661 gatgcaagca agattccaca actttttctc cgtactctaa tataaaatca tctaactgtg
      721 tatctataaa atcacaataa tcatatgaac taccaatata agcagttagc atatgtgtac
      781 atttatccat tttatctcta aacataatgt atttatcccc ctaaaaaact ttcatgaaaa
      841 acaaaaagaa aatatctttt tggcaactgg ctgacataac ttattttata agcgttagtc
      901 cctatagctt tgcgtcacta aatttctcta gttttgccga tttagtttta ttctacagat
      961 taataataca atagaattag ctattattca acatatctag ctgaaaaatt aataaattta
     1021 tatatgaacg ttaaattatt tgaaaaaata aaaagcccct ctgt
//
LOCUS       DAEPOG010000327         1040 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEPOG010000327 DAEPOG010000000
VERSION     DAEPOG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.332"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(402..578)
                     /locus_tag="KR432_003678"
     CDS             complement(402..578)
                     /locus_tag="KR432_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642181.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          412 a          115 c          126 g          387 t
ORIGIN      
        1 agttatacgt ttttattgac cagttatgca gttgatattg aatattctaa atattggtgt
       61 tattttatag gtggtttaaa catattaggg ggattttata tatgatttgt atgatttata
      121 gagaatgtat aaaatagtaa aggtttagag catgtttagt ggattgcaga atatgcatat
      181 agaatgattc ttgtaaatta agttagacat aggaaatata taatttgtat tggatgattg
      241 attttatggt gtatgctttt aacttctata atgggaaagt aaagatgtac tcccaataca
      301 tctttacttt agcagaagtt atagttactt ataaagtaca aaaaattata tatttattag
      361 agtagataaa tctaaaaagg tttttctaga aaaatttaat tttaattgta tctacttact
      421 aataataaga cttgatgtaa gcaggtttca actaccgttt ctccatattc tgatataaaa
      481 tcatctaact gtgtatctaa aaaattacaa taatcatatg aactactgat gtaagcagtt
      541 agtatatgtg tacatttgtc tatttctcca ataaacatat tatccatccc cctaaaaaat
      601 ttagtaaaaa taaaaagtcg cattctatta gtcaattaac ataatttata aaatttttta
      661 gtactagttt ctataactta actccttaag gttaaatatt cccagtttta ccaatttagt
      721 tttattctac aagtatataa tactagaaga tgcttcatta ttcaacatgc ttgtttgaga
      781 atagaaaata ttctaaaatt ataattgttt ttaaaaataa gattcaagat aaataaagtt
      841 atatgcaact atttaataat ctttccataa taaggacagt aggttctatt cttaaaaaac
      901 tccatcttta aatttaaaga ataactacaa tttgaacaac aataaaattt ccaatcaata
      961 aaaatatcaa aaataataaa aataattgaa aatgtataat ttcccagaaa aataataaat
     1021 gtaactccaa taataaacac
//
LOCUS       DAEPOG010000328          994 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEPOG010000328 DAEPOG010000000
VERSION     DAEPOG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.334"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>994
                     /locus_tag="KR432_003679"
     CDS             <1..>994
                     /locus_tag="KR432_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBH0642182.1"
                     /translation="TINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTILTKKGAL
                     IATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGENCTVITR
                     KGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLGISVGDFV
                     CLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYISNYEEIGH
                     GVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKLVDVAEDN
                     NLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIENTLKL"
BASE COUNT          384 a          129 c          181 g          300 t
ORIGIN      
        1 actataaatt taatgaaaaa atatttggac attccaagcc cagctggata tactgaaaat
       61 gcagttttag aaatcaaaaa agattttgaa aatttaggtc ttgacactat tcttacaaaa
      121 aaaggtgcac ttatagctac tcttcctggt gaagatgatt caaatcaaat aacaatatct
      181 gcacatatgg atacacttgg agctatagta aaagaaatat catctgatgg tactcttaaa
      241 taccataaag ttggtggtgg atgttgggca gctatagagg gagaaaactg cactgttata
      301 actagaaaag gtaaaaaaat cagaggaagt gttgtattta agtatgcttc tactcatata
      361 tatggtcaaa caaaagctgc cactgaaaga aatgaagaaa caatgattat tagacttgat
      421 gaaaaagtat ttacaaagca agatgtatta gatttaggaa taagtgttgg ggattttgta
      481 tgcctagacc atagatttga ggctacagaa tctggtttta taaaatctag atatatagat
      541 aataaatcag ctgtagcaat ggttcttgaa atatgtagat attttaaaga aaacaattta
      601 acgcctaagc atactactaa cttttatata agtaattatg aagaaattgg acatggtgtt
      661 tctaaatgtt tacctgaaaa aacaaaagaa tttgtagcaa tagatatagc acctactgga
      721 ccaagtcaaa catctagtga acatggtgta actatagttg caaaagataa tttatcatta
      781 tatgatttta atttaagaaa caagcttgtc gatgttgcag aagacaataa tttagactac
      841 gctgtcgatg tatttacttt ctatggttca gatgcttcag aagctattcg ttggggagaa
      901 gatgttcaaa tagcatgtgt tggtcctgga acaaacaata gtcaccatta tgaaagaaca
      961 catatagaag ctattgaaaa tacactaaaa ttac
//
LOCUS       DAEPOG010000329          954 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEPOG010000329 DAEPOG010000000
VERSION     DAEPOG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.335"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..767)
                     /locus_tag="KR432_003680"
     CDS             complement(<1..767)
                     /locus_tag="KR432_003680"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0642183.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYN"
     gene            complement(794..>954)
                     /locus_tag="KR432_003681"
     CDS             complement(794..>954)
                     /locus_tag="KR432_003681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642184.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
BASE COUNT          304 a          155 c           91 g          404 t
ORIGIN      
        1 ttattataaa aataaatata atcatttatt agactaatca tttcctttgg atctgtagta
       61 tcaattaaat atattaattc agatttaaaa tgactgaaaa aactttctat aggagcatta
      121 tcccagcagt tgccccttcg ggacatagat tgtataatac cattatcttt aagcctttta
      181 gagtaagatc tacttgtata ttgtgaacct tgatctgtat gtaaaataca atctttagct
      241 aaattcatat ttattagttg atttactgtt ttattaacta atttagtatc catttttgta
      301 cttatctcat atgccactat ttcaccatta aataaatctt tggctacatt catgtataag
      361 aattttttac cgataggtat gtatgttata tccatacaaa ttttttctaa tggttgatta
      421 gctctaaatt cacgatttaa tatattatca tatacctcat atgatttaaa cttacgtcta
      481 tatactttct ttctaataat agatttaatt cctagttctt tcatcaacct atatactttc
      541 ttatgattaa tagggctatc aagtatttta tttaaataag tacaaattct ttttctaccg
      601 taagttcctc tatatttttt atgtatttca attatataat ccttaataat tctatctttg
      661 atacccctat cggaagattt tttagaataa cttagccatt tatagtaacc gcttcttgac
      721 acacctatca acttacacat actagaaata ctatacttcc tagacataag tttaattgtt
      781 ttaaatttat cgcttacttt gacttgtcca atagggtact taactttttt aaaaattcat
      841 tctccatgcg taagtacttc aattcttctt caacagattt aaattttcta ttatcatttt
      901 ctagactaat atttttaaat ctgcctctag tttcctcatt aaatgcagtt ttac
//
LOCUS       DAEPOG010000330          938 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEPOG010000330 DAEPOG010000000
VERSION     DAEPOG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.336"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(150..803)
                     /locus_tag="KR432_003682"
     CDS             complement(150..803)
                     /locus_tag="KR432_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0642185.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
BASE COUNT          289 a          150 c           98 g          401 t
ORIGIN      
        1 ttcattgatt tatatatgtt aatttaaata tattattcta tcgtagtgcc ttagagtttt
       61 tatttgtatt taaagttaaa tttattcttg tttttccttt aatatttaat agtaaaataa
      121 caaaattata aaaaaaataa agagaaactt tataaaagct tctctttttt catctctgct
      181 ataggtatcg ttttccaatg ttctctcaaa actctttcaa cattttctaa atcattctcc
      241 ctcataaatt ttactacttc aatatgttga ttattgctgt atgtcaacat tttatataac
      301 tcatcttcat tatcactagt atatgacttt gacataaaat tatatcttaa agagttcaat
      361 agatttacta acacctgatt atctgattta gttatgtata tatcgtgaaa ttcattactt
      421 aattttaaat agctattgta atttttatac tttatagaga ccgccatcat ttctgttaat
      481 tcttccattt ttactaggtc tgattcagtt aaattttgta atgctgaaga accggctaac
      541 gcatccaata ctccaattac ggaataggtg tctaatttct ccttttcctg aaattctttt
      601 actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa cctagttaat
      661 gcttctctag ctggtgttct actaactccg atagatttac aaatctcaga ttcagtaatt
      721 ctctgattag cttcatactc tccattttgg attttctttg atatatagtc atatacttga
      781 gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata aaagttaaat
      841 ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata atatcacaat
      901 tatgatattt tttatacttc tttgtaatat tttgaaac
//
LOCUS       DAEPOG010000331          937 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEPOG010000331 DAEPOG010000000
VERSION     DAEPOG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.337"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..>937)
                     /locus_tag="KR432_003683"
     rRNA            complement(<1..>937)
                     /locus_tag="KR432_003683"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          195 a          280 c          193 g          269 t
ORIGIN      
        1 aaccttcccc tggaaacctt gggtttacgg cctgtgggat tctcacccac atctcgctac
       61 tcatgccaac attctcactc ccatactgtc cacatgtcct tacggtcatg cttcaacccg
      121 tatgggaagc tcccctaccc atcattactg atgccgtagc ttcggtagta agttttagcc
      181 ccggaaatct tcggcgcagg atcactcgac cagtgagcta ttacgcactc tttgaatgag
      241 tggctgcttc taagccaaca tcctggttgt ctgtgcaatc ccacatcctt taccacttaa
      301 cttacattta gggaccttag ctgacgatct gggctgttgc cctttcgact atgaatctta
      361 tcacccacag tctgactccc aagtataaga tgacggcatt cggagtttga tagtcttcgg
      421 taggtgcaat accccctagg acattcagtg ctctacctcc gtatctctca ccttgaggct
      481 agccctaaag ctatttcggg gagaaccagc tatctccggg ctcgattgga atttcaccgc
      541 tacccacaag tcatccccga gcttttcaac gctcgtgggt tcggacctcc acgaaatttt
      601 actttcgctt catcctgctc atgggtaggt cgcccggttt cgggtctacg tcaagtaact
      661 gaacgctcag ttaaaactcg ctttcgctac ggctccgcac cttaagtgct taaccttgct
      721 acttaacgta actcgttggc ccgttctaca aaaagtacgc ggtcacacaa gaaatgtgct
      781 tccacagctt gtaagtgcag ggtttcaggt tctatttcac tcccctcccg gggttctttt
      841 cacctttccc tcacggtact atacgctatc ggtcactagg tagtatttag ccttggaggg
      901 tggtccctcc tgcttcccac agggtttcac gtgtccc
//
LOCUS       DAEPOG010000332          927 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEPOG010000332 DAEPOG010000000
VERSION     DAEPOG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.338"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            3..79
                     /locus_tag="KR432_003684"
     tRNA            3..79
                     /locus_tag="KR432_003684"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="KR432_003685"
     tRNA            90..164
                     /locus_tag="KR432_003685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="KR432_003686"
     tRNA            174..247
                     /locus_tag="KR432_003686"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
BASE COUNT          332 a          102 c          160 g          333 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa cttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataaatt atagtaaact tttatattgt ttaaaaagac
      301 acaggaaaca tgtatattac ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tacagtatat tctaatagag ttacagtata ttcattaata ttgtaaacat acggagggta
      421 atgtataata gtctacaaga tgtttatgaa ggcatcagtt aaatataact aattagataa
      481 gagttatata cattatattg tatactttat ggtatttata tattgaaata acttatgaaa
      541 ttatttataa agagatttat gagcatattt ttgtctaaac aataagtata agaaattata
      601 aaaaattaga gactatagtg aattgacaat ttaatgtaat atttataagt tatactaagt
      661 aaattattat actgtgaaat ggatatataa ctttaggtat tgtgtattat tttcactgta
      721 ggagattata tctagttata cataataagt tttaatgata gtttatagta agatattaaa
      781 ataaatttat ctaatctaga tatgaaataa gttattactt taatataaaa atgaatctaa
      841 tggaataata ttgatgataa gtaatattta gattttgtat ttgtttatag ttaataagtg
      901 gattatagaa tttataataa ttaatat
//
LOCUS       DAEPOG010000333          899 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEPOG010000333 DAEPOG010000000
VERSION     DAEPOG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.339"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            115..876
                     /locus_tag="KR432_003687"
     CDS             115..876
                     /locus_tag="KR432_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0642186.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
BASE COUNT          386 a           95 c          137 g          281 t
ORIGIN      
        1 tccagtaaag atattttttt atatttttat taaaagatat aaatttttat attttaatat
       61 attgacattt acgttgcgta aaagtgtata tttaatttat aggaggtatt acttatggaa
      121 tatacagttc aaaaattaag taaactagct ggtataagta ctagaacact tagatattat
      181 gatgaaatag gtcttcttaa gccacttaaa ataaattctt caggttatcg tatatatggt
      241 caaaatgaag ttaataaact tcaacaaata cttttttata gagaattagg aattaactta
      301 gaaaatataa aaaacattat aaattcacca aacttcgata gtttaagtgc gttaaaagaa
      361 caccataata aattactagc caaaagaaag caaattgatt tactaataga aaatgtaact
      421 aaaactatag ctctgaagga gggaaaatac actatgacag atatagaaaa atttgaaggt
      481 tttaaagaaa agatgattga tgaaaatgaa aaaaattatg gaactgaaat tagagagaaa
      541 tatggtaaag atattataga tacatcaaat aaaaagctta aaaatatgtc taagcaagat
      601 tatgaaaatt ggcaaaacct aaatatcgaa ataattagta aacttaaaaa agcttttaaa
      661 actggtgatg cctctagtga actggctcag gaagttgcaa aattacatca taaatggttg
      721 agttacactt ggaacacata ctctaaagaa gctcattcct ctctagcaca gatgtatgta
      781 tatgatgaac gttttacatc atattatgat agagaacaac ctggattagc taagttttta
      841 agggatgcta tagtaatata tacaagagat aaataatata aaataataaa ggtggtgtc
//
LOCUS       DAEPOG010000334          894 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEPOG010000334 DAEPOG010000000
VERSION     DAEPOG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.340"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            13..726
                     /gene="rgbR"
                     /locus_tag="KR432_003688"
     CDS             13..726
                     /gene="rgbR"
                     /locus_tag="KR432_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBH0642187.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT          404 a           60 c          146 g          284 t
ORIGIN      
        1 aggaggcatt ttatgattaa gatagcagtt tgtgaagatg aaaaagaaac acaactttta
       61 atagaagatt accttgagaa tatattaaaa gatataagta tagaatatga gatacaaaag
      121 tatatatctg gagaagagtt actggaaagt aatttaaaag atatagatat attacttctt
      181 gatataaaaa tggaaaaatt aaatggaatg gatactgcaa gaaagattag agaagtagac
      241 gatgaaatgg aaataatatt tgtaacttca ttaatagatt atgtgcaaga aggttatgag
      301 gtaagagctt ataggtattt actaaagcca atagagttgg aagaactaaa aaaacatgtg
      361 ctaacttgta tcaaagatat tgaaataaat aaggaaagtc atattacaat aaaaaataag
      421 tctaatacat ataagattta tttaaatgaa ataaaatata tagaagttca aaaaaaagat
      481 atgctaatac acacaataaa caagaatttt gatataaaat atagtttagg taaaatagaa
      541 aaagaattaa atccatataa atttataaga tgccataaaa gttttatagt aaatttaaga
      601 tatgttgaaa atataaagcc taatgctgca atactggaaa gtggagaaga ggtacctatt
      661 agtagatata gatataaaga agttaaggag aaatttttaa aatttcttgg tgatacaata
      721 tgctaaattt tatgaatgag ctattagtag ttttatctat atttatacag ttttctataa
      781 tgaacggaat tttaatttat aaaaaggata gtaaaaaaaa tgaaaaaata gtattctata
      841 ttgtcttgtt tcttatttat attataattc agataagtag attatttaat ggag
//
LOCUS       DAEPOG010000335          885 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEPOG010000335 DAEPOG010000000
VERSION     DAEPOG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.341"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(132..806)
                     /locus_tag="KR432_003689"
     CDS             complement(132..806)
                     /locus_tag="KR432_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0642188.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
BASE COUNT          315 a          141 c           84 g          345 t
ORIGIN      
        1 aaaaacatct acaggagaaa atataataac tgatactgta aaaattaaga aagtaaaatt
       61 gtatactaaa aaacctagtt tatccttgag aaaatcttta aagctcataa attcacctac
      121 ttagaattgt tttaactgat tatataaccc aatcctcttt ttgtttctat aaaatcttct
      181 aaaccaattt catttaattt tgagcgtatt ctatttacat tgacagttaa tgtattatca
      241 tctacaaacc aatcattatc ccataatttt ttcatgagtt tattcctact tactatctgc
      301 cccttatatt tcataagctc atgaagaatt ttgatttcat tttttgttag ctcaatagta
      361 ttatcttcat agtttattgt tgcagttgat aaatctaaag taacaccatt gtgaataatc
      421 tgatttgagt tattatcaac aaaattataa gttcttctta aaagggcatt aactttagct
      481 actaagacat ctacagaaaa aggtttttgt atataatcat ctgcacctaa atttacaccc
      541 attattacat ccatattaga atttcttgaa gaaataaaaa ttataggaac ttttgaaatt
      601 tttcttattt cattacacca atgaaaacca tcataaaatg gaaggtttat gtccaataaa
      661 actaattctg ggttatagtt tttgaactct tctattatat tttcaagatt ttctatttga
      721 tgaacatcat gtccccattt agtaagacac tttgctatga tatttttcaa tgagacgtca
      781 tcttcaacta ccattatttt aaacattctt acctcctaat tcaaaatagt tatattaaca
      841 taactatatt ctacttgata aattaaaata tttctacctt attac
//
LOCUS       DAEPOG010000336          871 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEPOG010000336 DAEPOG010000000
VERSION     DAEPOG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.342"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          360 a           89 c          141 g          281 t
ORIGIN      
        1 tcaatgagaa cataaaaatt ttatactata caaatgtatg caaatattgg cactaaaatt
       61 gctctataaa aattataaat agtaatatga ataaaatttt tactataaaa ataatattac
      121 tattttgctt atagatatat atatctataa atagatagaa acttgtatat tttatgttgc
      181 agatacaaat atagtaaagg agagtacatt ggtacaactg agatacatag cttattaagt
      241 ttttctgtct tatgagaatt atattataaa atattcataa atattttgag tttaaattaa
      301 ataattattt tatgcttaga ttaattgata tttaattaat ttaataacaa aaacagtgaa
      361 agagtaaata atattattta cagggggtaa agcaaagtat gttaaataaa ttgtactttt
      421 ggccccgtac atagagaaat ctatgtagga cacaacttta aaaccagtaa atcctaaagc
      481 cttgcaacta caataaaaga tagaaagaaa cttttatgaa tgtggtgaaa accagaaaca
      541 atagtgagga tggtatatgg tgagagccta agtacttaaa aatggacatt tggtcgccaa
      601 gcctagaaat aggaaggtca aacgactatc tcgagagaga gtacactata agcgattgat
      661 agtggaaacg ggttgctctt agcaggtaat gctgaagatg aagaaatagt ctacgctcat
      721 atgaaagtat gaggtcacta gatttgaagt gacatctata aagtagcgtt tataggtaaa
      781 tgtttaaaac ttgaaacatg taaatactta tgaaaactta taacttataa tgattaagat
      841 tgaaagattt aaattgaatt aaatttacta t
//
LOCUS       DAEPOG010000337          844 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEPOG010000337 DAEPOG010000000
VERSION     DAEPOG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.343"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..798)
                     /locus_tag="KR432_003690"
     CDS             complement(<1..798)
                     /locus_tag="KR432_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0642189.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKE"
BASE COUNT          276 a          109 c           75 g          384 t
ORIGIN      
        1 ttctttatat tttccaatat tgtatttcat gttttccagt attattttaa gactagcaag
       61 aggagtttta agttcatggg atgcaccttt aaaaaagtca tattttaact tttctaattt
      121 taaaatttct ttatttttaa attccaaatc gtcaattgtt cttaataaag tagaatacaa
      181 atcattaatt tgttgtttta attctccaac ttcatcatta gagctaactt ttaaacgcgt
      241 ttttttatca agtttcatca ttttatcagt aacaattttt atttcttgta tattattttt
      301 tattgatttt gcataaatta aagaaataat agcagaaaat aaaattgata ttaataaaga
      361 atatggtaaa aatttaaggc ttaaatcttt cgcatctttt tgcatatcag ctgtagaaac
      421 aaattgtaga tttgtttttt taccatcatt aagctttatt tctctttctt caattatcaa
      481 agaattacta tcactttcta aattaacatt tatattatct ttgatttgta tttcattctt
      541 attatttttt tcttttataa atgcttttat ttcactactc ttagaataaa gctccaaagt
      601 ttgttctata tattttattt cttttccatt catattacta gaaatttcat ttgccatatt
      661 atgaatcttt tcttttctcg tctccaaata tgttttagga aagataaaaa aaactaatga
      721 atgaactaat ataattatta ttccaagaac agaaaatatt tgtatgaaca tttttggaaa
      781 tatctttaat tttttcattt tctctccaat ttatatccta catttcttat agtagttata
      841 cagt
//
LOCUS       DAEPOG010000338          818 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEPOG010000338 DAEPOG010000000
VERSION     DAEPOG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.344"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..758
                     /locus_tag="KR432_003691"
     CDS             <1..758
                     /locus_tag="KR432_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBH0642190.1"
                     /translation="TGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTG
                     YTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDN
                     IYYFGNDSKAATGWATIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGP
                     NGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINSKVYYFM
                     PDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT          280 a          110 c          147 g          281 t
ORIGIN      
        1 ttaccggatt gcgaactatt gatggtaaaa aatattactt taatactaac actgctgttg
       61 cagttactgg atggcaaact attaatggta aaaaatacta ctttaatact aacacttata
      121 tagcttcaac tggttataca attattagtg gtaaacattt ttattttaat actgatggta
      181 ttatgcagat aggagtgttt aaaggacctg atggatttga atactttgca cctgctaata
      241 cggatgctaa caacatagaa ggtcaagcta tacgttatca aaatagattc ctatatttac
      301 atgacaatat atattacttt ggcaatgatt caaaagcggc tactggttgg gcaactattg
      361 atggtaatag atattacttc gagcctaata cagctatggg tgcgaatggt tataaaacta
      421 ttgataataa aaatttttac tttagaaatg gtttacctca gataggagtg tttaaaggac
      481 ctaatggatt tgaatacttt gcacctgcta atacggatgc taacaatata gatggtcaag
      541 ctatacgtta tcaaaataga ttcctacatt tacttggaaa aatatattac tttggtaata
      601 actcaaaagc agttactgga tggcaaacta ttaatagtaa agtatattac tttatgcctg
      661 atactgctat ggctgcagct ggtggacttt tcgagattga tggtgttata tatttctttg
      721 gtgttgatgg agtaaaagcc cctgggatat atggctaaaa tatatgtttg acaaaaaatt
      781 attcctgtga tactcagaaa ttatttttat ataataaa
//
LOCUS       DAEPOG010000339          787 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEPOG010000339 DAEPOG010000000
VERSION     DAEPOG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.345"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            226..669
                     /locus_tag="KR432_003692"
     CDS             226..669
                     /locus_tag="KR432_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0642191.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT          309 a           65 c          122 g          291 t
ORIGIN      
        1 gtattgatat tctaggtaat tgctagagct gatattatag atgaatatta gtattgatat
       61 tctaggtaat tgctagagct gatattataa tttattatta atattaatat tctagggaat
      121 tatcactata ttttaaaaag taaaaatata tatcaaaatg aactaaataa gtactgtaag
      181 aagtttgtta aattaaggtt tgaataaagt gagggtggag aacttatgat agaaataaaa
      241 actatagaaa taaataaaaa aagtcaaatt gaccttttaa tgttagccga cccatgtgaa
      301 gaaatgattg ataaatatct caataaagga actatgtacg cattgtatga taataaagaa
      361 cttacttgta tagcagttgt aaatgaaatt tccaaagaaa tatgtgaatt aaaaaacatt
      421 gctacatatg agcattttca aaatatgggt tatgcctcaa agatgattta tcatctttta
      481 gatgtttata gtaaaaaata ttcatcaatg gttgttggta catctgaaag cggagttcct
      541 ttttatgaga agtttggatt tgtttattca cataaaatta aaaacttttt tgtagataat
      601 tatccagaac caatctttga aggagaatta caatgtgttg acatgttgta tctatcatat
      661 aacttttaga taggataata tttttaggta gatataatta attaattcat ataattatcg
      721 gaattataat ttataaatta tgtataataa gagtataatt tatttagaag ctgttatatt
      781 gtattat
//
LOCUS       DAEPOG010000340          786 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEPOG010000340 DAEPOG010000000
VERSION     DAEPOG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.346"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..603
                     /locus_tag="KR432_003693"
     CDS             <1..603
                     /locus_tag="KR432_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0642192.1"
                     /translation="AKLKRLQKKEILFYEKDSLDSVYILLEGKVSIFKISENGERKVI
                     FILNSGEVINDITFDSSKNSTVGCEAFENSIVAYYSIEEFLEIMQNDFQLTKDIISYM
                     ERRIRRLYRQLKNSISIKVDKKLAAKLYRLSREFGVCCGEWTLLNVNITVTYLADMLG
                     CKRETVSRMIKVLQKEELIKIDNKGFYVKETELSRYFKNN"
BASE COUNT          322 a           59 c          122 g          283 t
ORIGIN      
        1 gctaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga tagcttagac
       61 agtgtttata tacttttaga aggaaaggta tctattttta agataagtga aaatggagag
      121 agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac atttgactct
      181 tctaaaaact ccactgttgg ctgtgaagca tttgaaaatt caattgtagc ttattatagt
      241 attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga tattatttct
      301 tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat atccataaaa
      361 gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg agtttgctgt
      421 ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga tatgcttggc
      481 tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga acttattaag
      541 attgataata aaggttttta tgttaaagaa actgaattat caaggtattt caaaaataat
      601 taattatgat taagtaaaag tattattaga attttaatta aataaaaata ttattaagag
      661 aatctattta tagattcttt tttattgtct aatttattaa tttttatttt accctattga
      721 attgtataaa aaagcaaata agattcttta ttattcaaaa aattgagtag ataatagttt
      781 tattgt
//
LOCUS       DAEPOG010000341          780 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEPOG010000341 DAEPOG010000000
VERSION     DAEPOG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.347"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            190..>780
                     /locus_tag="KR432_003694"
     CDS             190..>780
                     /locus_tag="KR432_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0642193.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          344 a           75 c          151 g          210 t
ORIGIN      
        1 ttcaaaataa aaactaaata aaattaaata aaaccaaata aaattaaaat aaagtctaaa
       61 taaaaattta ataaataaaa tataaagaaa taaaaaatca atagattaag tgaaatgcta
      121 ataaattaaa aaagtgaaat atcaaaataa aacgtcaaca aattaaaaat tatatggggg
      181 aatatcaaaa tgttaaatga aacaagaaaa aaacaattaa ctgaagtatg ccaagactta
      241 ataagaaatg ctagttattc aggtcaagaa gaaaacgtag taaaagctat agaagaaaat
      301 tttgctttac taggttttga tagttggtca agagatagat atggaaatat agtaggatgt
      361 ataaaaggaa ataaaccagg taaaaaaata ttatttgacg ggcatataga tacagttcca
      421 gttccagatg caagtaaatg gagtgttcct ccttttgaag cgagaatagt agatgggaaa
      481 atatatggta gaggaacttc tgatatgaag gggcaagtaa gtgctatgat ggctgctgtt
      541 tcatactttg cagaagatac taacaaagac tttgaaggtg aattgtatgt agcaggtgtt
      601 gttcatgaag aaatatttga aggtgtttca gctagagaga taagtaaggc tgttcaacct
      661 gattatgttg taataggtga atcttcagaa ttaaacttaa agataggtca aagaggtaga
      721 ggagaaatag tagtagaaac ttttggtaaa ccagctcact ctgctaatcc agaaaaaggt
//
LOCUS       DAEPOG010000342          760 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEPOG010000342 DAEPOG010000000
VERSION     DAEPOG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.348"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>760
                     /locus_tag="KR432_003695"
     rRNA            <1..>760
                     /locus_tag="KR432_003695"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAEPOG010000343          752 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEPOG010000343 DAEPOG010000000
VERSION     DAEPOG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.349"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="KR432_003696"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="KR432_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH0642194.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAEPOG010000344          746 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEPOG010000344 DAEPOG010000000
VERSION     DAEPOG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.350"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            15..105
                     /locus_tag="KR432_003697"
     tRNA            15..105
                     /locus_tag="KR432_003697"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ser,seq:gct)
     gene            114..190
                     /locus_tag="KR432_003698"
     tRNA            114..190
                     /locus_tag="KR432_003698"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            276..351
                     /locus_tag="KR432_003699"
     tRNA            276..351
                     /locus_tag="KR432_003699"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Trp,seq:cca)
     gene            412..488
                     /locus_tag="KR432_003700"
     tRNA            412..488
                     /locus_tag="KR432_003700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Pro,seq:tgg)
     gene            495..571
                     /locus_tag="KR432_003701"
     tRNA            495..571
                     /locus_tag="KR432_003701"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Ile,seq:gat)
     gene            575..650
                     /locus_tag="KR432_003702"
     tRNA            575..650
                     /locus_tag="KR432_003702"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:608..610,aa:Phe,seq:gaa)
     gene            658..734
                     /locus_tag="KR432_003703"
     tRNA            658..734
                     /locus_tag="KR432_003703"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Met,seq:cat)
BASE COUNT          175 a          171 c          215 g          185 t
ORIGIN      
        1 aaatgactga atttggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
       61 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccattcaa aaacgaggtg
      121 tagcgcagtt tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc
      181 acctcgacca acttaagctt ttagttcagt tggttagagt gcccaactca tggctagtaa
      241 ttctagggtt tgagtccatg aaggttattc ttcatagggg tgtagctcag ttggtagagc
      301 gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc cacccctgcc aaataaaaag
      361 gacttgaaaa aagttggatt ttagagtagt ataagatagt aaataagatt tcgaggtgta
      421 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
      481 ctcgaccaag aaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag
      541 gtcgctggtt cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca
      601 ggggactgaa aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc
      661 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      721 tattaccgct accatctaac tatatc
//
LOCUS       DAEPOG010000345          723 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEPOG010000345 DAEPOG010000000
VERSION     DAEPOG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.351"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>723
                     /locus_tag="KR432_003704"
     CDS             <1..>723
                     /locus_tag="KR432_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0642195.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAEPOG010000346          703 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEPOG010000346 DAEPOG010000000
VERSION     DAEPOG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.352"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>703
                     /locus_tag="KR432_003705"
     rRNA            <1..>703
                     /locus_tag="KR432_003705"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          147 c          203 g          146 t
ORIGIN      
        1 acctaagctg aggccgaaag gcgtaggtga tggacaacag gttgatattc ctgtactacc
       61 gataaccgtt tgagagaagg gatgacacag taggataagc taagcacact gttggttatg
      121 tgtgcccaag cattgaggca gtcaaagtag gcaaatccgc tttgataatg ctgggatgtg
      181 atggggagcg aaatttagta gcgaagtagc tgatttcaca ctgtcaagaa aagtctctat
      241 cgaggttaaa ggtacccgta ccgcaaaccg acacaggtgg gtgaggagag tatcctaagg
      301 ccagcgagag aactgttgtt aaggaactcg gcaaaatgac cccgtaactt agggataagg
      361 ggtgccacca tcaggtggcc gcagagaata ggcccaagcg actgtttacc aaaaacatag
      421 gtttctgcta agtcgcaaga cgatgtatag gagctgacgc ctgcccggtg ctggaaggtt
      481 aaggggatct gttagagcaa tcgaagcagt gaacttaagc cccagtaaac ggcggccgta
      541 actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag
      601 gcgtaacgat ttgggcactg tctcaacaac agactcggtg aaattgtaat tccggtgaag
      661 atgccggata cctgcgacag gacggaaaga ccccatggag ctt
//
LOCUS       DAEPOG010000347          692 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEPOG010000347 DAEPOG010000000
VERSION     DAEPOG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.353"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            89..421
                     /locus_tag="KR432_003706"
     CDS             89..421
                     /locus_tag="KR432_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBH0642196.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
BASE COUNT          282 a           65 c          123 g          222 t
ORIGIN      
        1 tctattttac agataaataa gaataaactt ataatataat tgtatctgta aaattttgta
       61 aaatataaaa agtatagggg gaataaaaat gaaagtagct tatattttta gtagtactaa
      121 tacacataaa attctagaca aaatgataat accacaactt gaacaaggaa ctcatggagt
      181 agacgttcta gggatgtttt tctttatgga taatacctta tttttatcta aaggtaatcc
      241 agtaggcgag agactaagta aattacatga aaaaactgat atgataataa tggcttgtga
      301 ccaatgtgct atagaaagag gtatagagaa taatttggtt gatggagcta caattggatg
      361 tttcccaaac ctatatgctt gtttaggcag tgcaggtgtg gaccaagtta taacgttata
      421 aattagtata ggatacaatc catagaaaaa tttattttct gtggattatt ttaagtaaaa
      481 gataaattgt ttaaagtagg ggatgaacaa atgtctaaaa ttttagttga atttataaac
      541 acttgaccat cttgtgatga acatggagtg tttataaaaa aaatgtcaga aaagtatgct
      601 gataaattgg aaataaaatt atatcaagca gggaaagatt tttcgtatat aaaaaaatat
      661 gggattataa caaaaggaac tttaataata aa
//
LOCUS       DAEPOG010000348          671 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEPOG010000348 DAEPOG010000000
VERSION     DAEPOG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.354"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            447..>671
                     /locus_tag="KR432_003707"
     CDS             447..>671
                     /locus_tag="KR432_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642197.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKP"
BASE COUNT          301 a           69 c           76 g          225 t
ORIGIN      
        1 tatctcacac ccattttcat tttggataga ttataattag ttagacaata aatattttgt
       61 catgatatta ttatatatag tgatacaaat agaaatactt ttatcatgac tagaaaaaca
      121 aagattacac aaaggattta aaaatcaaac aacttcaatt aaaataaatg gttaaaataa
      181 cttctaaatc aactacataa acaataaaag aacataaagt agttaaatct acaattataa
      241 aattggtaag actataataa cttaaataaa attaaagtta acaaagacta ttaaagttag
      301 caaatattcc tctacctaaa taattgcttt ttataaagag ttatttattt taatttattt
      361 taattgaaat acgattttaa ctagaacaat agattaatgt aatatttaat ataaaataat
      421 tataataatt gaaaaggaag taatctatga tgaaaatagc agtcataaca accgaatttt
      481 taaaagagtt tgtagataat tctataaaaa agttaaatat taatgcagaa attgaaatat
      541 atatctatag agatttttct catgttggag atttatattt agaaatagaa gataggtttg
      601 atggatttgc agttagtggc cccatcccca aaaaagctat tacaaaaaag gctggaacta
      661 ttaaaaaacc t
//
LOCUS       DAEPOG010000349          653 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEPOG010000349 DAEPOG010000000
VERSION     DAEPOG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.355"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            37..>653
                     /locus_tag="KR432_003708"
     CDS             37..>653
                     /locus_tag="KR432_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0642198.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPEYLNVWELQ
                     GITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNG"
BASE COUNT          285 a           92 c          109 g          167 t
ORIGIN      
        1 agtgtcaaat ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gacatgccat tggatacaac tctaaattac aatcaatcaa ctggggcata tgaaggaact
      121 atcaattata accaagaccc agaatatcta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac ggacaagacc tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac acaggaatgt ataattgaag acataacttc tagaaaagat
      301 gtaaataaat atttgagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tatgaaacag cagttaaaat aagtaaagag ggctggaaaa atggttcaga taaagtagtt
      421 ataataaatg gagatgtaag tatagatggc attatatcaa ctccgctggc aaccacatat
      481 aatgctccaa tacttttggt tgaaaaaaac aatgtgccta atagtgtaaa atcagaatta
      541 aagcgcctaa accctaaaga tataattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtttaaa tgg
//
LOCUS       DAEPOG010000350          653 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEPOG010000350 DAEPOG010000000
VERSION     DAEPOG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.356"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            37..>653
                     /locus_tag="KR432_003709"
     CDS             37..>653
                     /locus_tag="KR432_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0642199.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKG"
BASE COUNT          289 a           82 c          107 g          175 t
ORIGIN      
        1 agtgtcaagt ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gatatgccat tggatacaac tctaaattat aatcaatcaa ctgagtcata tgaaggaact
      121 atcaattata ataaagaccc agaatattta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac aaacaagaac tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac gcaggaatgt ataattgaag atataacttc tagaaaagat
      301 gtaaataaat atttaagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tacgaaacag cagttaaaat aagtaaagaa ggctggaaaa atggttcaga taaggtagtt
      421 ataataaatg gggatgtaag tatagatggc attatatcaa ctccactagc aactacatat
      481 aatgctccaa tacttttggt tgaaaaaaat aatgtaccta atagtgtaaa atcagaatta
      541 aagcgtttaa atcctagaga tgtaattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtctaaa ggg
//
LOCUS       DAEPOG010000351          646 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEPOG010000351 DAEPOG010000000
VERSION     DAEPOG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.357"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            114..599
                     /locus_tag="KR432_003710"
     CDS             114..599
                     /locus_tag="KR432_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0642200.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          290 a           51 c          108 g          197 t
ORIGIN      
        1 gtaataaagg tcatgtcagc cagttgccaa aaagataata attctttttg ttaaaagtat
       61 ttttagagtt atatatttta ataacttaat atttggcaag aggagggctt aagatgatat
      121 ctgaattaat gttaaaaaat tttggcaaat ctagcatcaa ttttaaaact aaaattaaat
      181 tgaatttaag agaaaaacca gacataaata gtttgaaatt aaaaagtata ccagaaggta
      241 aaattgttaa gttaaaatgt gtagatggaa tatgggcaga agttgagagt aattatgaca
      301 aaggctggtt actttataaa tatttagaaa gattaagtaa tgctaaaaac aatagtaatg
      361 atacaataaa aaatagaaaa aagaattaca taggaaatat aagaacaaat ggattaagtt
      421 tagaacttag aaatgataga acattagaat ctaaagttat aactacaatc cctgatggtt
      481 ttaaagttga agtatgttat agcgttggaa aatgggcaag agttaatatc aataaaaatg
      541 aaaaacgata ttctggatat gtatataatc aatacataga agagcattta agtttataat
      601 acgatactag tgagctttaa atatgttaat aaatcacaaa aaagaa
//
LOCUS       DAEPOG010000352          643 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEPOG010000352 DAEPOG010000000
VERSION     DAEPOG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.358"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..421)
                     /locus_tag="KR432_003711"
     CDS             complement(<1..421)
                     /locus_tag="KR432_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBH0642201.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIV"
BASE COUNT          227 a           95 c           33 g          288 t
ORIGIN      
        1 ctactatcaa gtctttttta ctttcattag aacctcttag ttcttccaat aaatttcctt
       61 ttttatctat ataacaataa ttatcttcat cttttaatac tgcaaatatt tctttttctt
      121 ttaaacttat aattatctta ttgggaagtt ttctttttat ttccacattt tctatatatg
      181 gattttttat aagtttattt ttcatatctt taaaattgta tgcaaaaata ttttcattta
      241 gatttatatt tagttctttc attatatttg atttggtcac cctcttattt ccaattacat
      301 ctattttctt tacatcaaat aaatcacttt ttacaattat acatatacaa aaagataaca
      361 taagtataaa aactaaaact atcatgacat tattcgtatt taactttctt ctttttttca
      421 aatataatca acccctttgg ttaataaata cacctttaga gcttgttttt cctacaaaaa
      481 aattataata tttaaagtca aatttttgca ttttattaca taacatccaa ataaggtatt
      541 aaattttata aataaatcaa tatttcatca aaataaacat agtatactac atactattta
      601 tatatttaaa tctgtataca tacaaataaa cccctaattt ata
//
LOCUS       DAEPOG010000353          636 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEPOG010000353 DAEPOG010000000
VERSION     DAEPOG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.359"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(57..>636)
                     /locus_tag="KR432_003712"
     CDS             complement(57..>636)
                     /locus_tag="KR432_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0642202.1"
                     /translation="TLENFNIINLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEE
                     NKNNILIEKCKLILFVVVLSLAIIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREY
                     IWNYLSNFIKSKSKIICITSILSGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIG
                     TVFGIVLGLVKYRFRDVGFCILLRSLFAIFIR"
BASE COUNT          269 a           95 c           49 g          223 t
ORIGIN      
        1 aaataaggtt gatttaaaat taaatttaaa acaaccttat aaaccgatta tactatctac
       61 ctaataaata tagcaaataa gctcctcaat aatatacaaa accctacatc tctaaaccta
      121 tacttaacta atccaagtac aataccaaaa acagtaccta tcataatttt tgaaataata
      181 acttcaaagg tgtagcttga attgttatac aatattacat aatttcttat aacatctatg
      241 tatcctatat tataaattcc agatagtata gatgttatac agataatttt actctttgac
      301 tttataaaat tacttagata gttccatata tattctctaa aaaataactc ttcaaatatt
      361 ggaactatca atacagacaa agtcatcata gttacattaa aaagtacata tcccttagta
      421 caataagtta caattattgc taaagataaa accacaacaa aaagtataag cttacacttt
      481 tcaataagta tattgttttt attttcttca aaagcttctg tgatttttct atttctaacc
      541 ttgttataca aagaattatc ttttaaaaat aaaattaacg ataaaccaac taatgtaaaa
      601 gaaattaagt ttataatatt aaaattttca agagta
//
LOCUS       DAEPOG010000354          631 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEPOG010000354 DAEPOG010000000
VERSION     DAEPOG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.360"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            61..>631
                     /locus_tag="KR432_003713"
     CDS             61..>631
                     /locus_tag="KR432_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0642203.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFL"
BASE COUNT          220 a           51 c           85 g          275 t
ORIGIN      
        1 tgtatttttt atgatatatt ttataaaaaa tataatttaa tcatttttga ggatattatt
       61 atgttagatg catttataac aattgtttct tctattatag aaatatggtc atgtaaaaaa
      121 gtttttgatt atactagtca gtcaaagacc gacttaaaaa aactaaatat gtattttctc
      181 ttttctgttt ttcttattat ttcttcttta tatataaata taggagctaa tagtagaatc
      241 ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt taatatatat
      301 aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa tggattaagt
      361 atgttattag tagcaacagt aagcaatttg gataatatga atgttttact tagtggcagt
      421 ttatatagat atgaagcaat attcttggga aagataatgt taggtatatt actttatgtt
      481 tataaggctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat atgtattcct
      541 atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa tcttgtaaaa
      601 atctctcctg aaagaagaac tgctttttta g
//
LOCUS       DAEPOG010000355          614 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEPOG010000355 DAEPOG010000000
VERSION     DAEPOG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.361"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>614
                     /locus_tag="KR432_003714"
     CDS             <1..>614
                     /locus_tag="KR432_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642204.1"
                     /translation="TGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSYDGILQNGY
                     ITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNG
                     KKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNTAEAATGWQ
                     TIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGP"
BASE COUNT          225 a           80 c           99 g          210 t
ORIGIN      
        1 ttactggatg gcaaaccatt gatggtaaaa aatattactt taatcctaat aatgctattg
       61 ctgcgactca tctatgcact ataaataacg acaagtatta ctttagttat gatggaattc
      121 ttcaaaatgg atatattact attgaaagaa ataatttcta ttttgatgct aataatgaat
      181 ctaaaatggt aacaggagta tttaaaggac ctaatggatt tgagtatttt gcacctgcta
      241 atactcataa taataacata gaaggtcagg ctatagttta ccagaataaa ttcttaactt
      301 tgaatggcaa aaaatattat tttgataatg actcaaaagc agttactgga tggcaaacta
      361 ttgatagtaa aaaatattac tttaatctta acactgctgt tgcagttact ggatggcaaa
      421 ctattgatgg tgaaaaatat tactttaatc ttaacactgc tgaagcagct actggatggc
      481 aaactattga tggtaaaaga tactacttta atactaacac ttatatagct tcaactggtt
      541 atacgattat taatggtaaa catttttatt ttaatactga tggtattatg cagataggag
      601 tgtttaaagg acct
//
LOCUS       DAEPOG010000356          589 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEPOG010000356 DAEPOG010000000
VERSION     DAEPOG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.362"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>589
                     /locus_tag="KR432_003715"
     CDS             <1..>589
                     /locus_tag="KR432_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642205.1"
                     /translation="EKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKE
                     KYNKSKENINDKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIAL
                     VCSLIAFVILIPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLN
                     NYIHWIKRKNIYIKANEKMEKRKKATLNDEDLNKDI"
BASE COUNT          242 a           50 c           99 g          198 t
ORIGIN      
        1 agaaaagtat agtaaatcaa aagaaaatat agatggaaaa ataatgaata cagatatatt
       61 aaatggtaaa tttagtaggt ttaaatcaaa tataaaaaag gattactcta tcttgaaaga
      121 tgaagctaag gaaaagtaca ataaatcaaa agaaaatata aatgataaac ttacaccaag
      181 tatagaagaa ggtgtagggg gattatctag acaagttata aagggattat tagtagtgtt
      241 atctttattt ctaattgttc cagcatttgc gtttgtatgt gctattgtat ctgttactat
      301 tgcattagta tgttcattaa ttgcttttgt tatattaatt ccatttgtga ttaaatttat
      361 agcaatggtt ccagatgtta tgatgttttg tattttcgca accatagcat ttgtagggct
      421 tgagatactt gcatggcaaa ttttattatt tgctataaaa ctaggtaaac aattactaaa
      481 taattatata cattggataa agagaaaaaa tatttatata aaggcaaatg aaaagatgga
      541 aaaaagaaaa aaagctactt taaatgatga agatttgaat aaagacatt
//
LOCUS       DAEPOG010000357          570 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEPOG010000357 DAEPOG010000000
VERSION     DAEPOG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.363"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            357..>570
                     /locus_tag="KR432_003716"
     CDS             357..>570
                     /locus_tag="KR432_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0642206.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYH"
BASE COUNT          247 a           55 c           69 g          199 t
ORIGIN      
        1 atattattac atacctaaaa aactttcata aaaacaaaaa taaaatatgc ttttagtaac
       61 ttgttaacat aacttataat ttattttata agcattagcc tatatagttt tgtgctacta
      121 aatttctttg attctaacga tttagtgcta ttctacaact aaaataatac aatagagcta
      181 tttattgttt ggtagcattg tttgcaaatt aaacaaatat atatatatga atattatttt
      241 acgtttaaaa aaacagacta aatatgtaga atatttggta aaacttacct ctagtttttg
      301 tcttaaaaag ctcctattat taatttaagt tagaagaatt aaggaagtga cataaattga
      361 aagaagaaat attaatagaa aaattattaa gagcagaatt aatagtaatg gagtatttat
      421 gtaaaaaaga cactctaatg cctaaaaaag aaattgtaaa agaaataaaa cagatttatg
      481 gatggcataa aagtacaata aaaatactac taaaaaggct agttgctaaa aggtatttgg
      541 ctagatatat tataaatttt caatatcatt
//
LOCUS       DAEPOG010000358          533 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEPOG010000358 DAEPOG010000000
VERSION     DAEPOG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.364"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          217 a           73 c           57 g          186 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac ccctttgaaa atattttaaa tggtttttta aatttggttt taaaaaatga
      121 ttttgatata aaaaaaccac acagtatata taactgtatg gcaaaaattc gaataagcca
      181 cacagtataa tccatgtggc tctatattat gatgataaaa taaaaacttt aacaacaatt
      241 actatagcca acatagatac aatccttaaa aatcataatc ctaaggggct tgtatctatg
      301 ttatgacaat ttgtcttaac ttaggtacaa accctaacta aattggctac taaaaaatcg
      361 tacttgtttg tttaataaag tcttcatatt tacctctcct tttaatttaa taattttgaa
      421 aattttgttt tctgttttgt atagtaatta gtttaataca gtaaaaaata aaagtcaact
      481 ataaattttg aaaaattatc aagttatcag aaaattgagt ttaaataaaa aaa
//
LOCUS       DAEPOG010000359          518 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEPOG010000359 DAEPOG010000000
VERSION     DAEPOG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.365"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            111..>518
                     /locus_tag="KR432_003717"
     CDS             111..>518
                     /locus_tag="KR432_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBH0642207.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILK"
BASE COUNT          231 a           47 c           67 g          173 t
ORIGIN      
        1 ttaaataaat atatacaatg tattaaaatg tatgcaactc tatttcaaaa ttattttgtg
       61 atatatatat gttataataa atgtataaaa ttttgattgg tgggttatat atgaatgcat
      121 ttattagaaa aagaaataaa aactatgttg tttacctgga atttgaagat gaaaagacag
      181 gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa aagggaagca agtaaaagac
      241 taaatgaggt aaaagaaagc ttatataatg atggtttttt agtaccaagc gaaattacag
      301 tgtcagaatt tctattagat tttctaaata aatatagtga aaatatatct ttagctacat
      361 ataataatta tgttagtatt tgtaaaaatt acattaatcc atctattgga aaatataaaa
      421 tacaagactt acatccggtt cacatacaaa actacataga taaactatct tataaactaa
      481 atcctcaatc aattaagatt catataaata tattgaaa
//
LOCUS       DAEPOG010000360          500 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEPOG010000360 DAEPOG010000000
VERSION     DAEPOG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.366"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..363
                     /locus_tag="KR432_003718"
     CDS             <1..363
                     /locus_tag="KR432_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0642208.1"
                     /translation="LMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITL
                     FKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISL
                     KKTNAKLVPKINQKKEVL"
     gene            364..>500
                     /locus_tag="KR432_003719"
     CDS             364..>500
                     /locus_tag="KR432_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642209.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          178 a           71 c           77 g          174 t
ORIGIN      
        1 cttatgattt caaacggact tatagcttta tctggtagtc ttatggctca gttcttggga
       61 ttctcagatg taaatatggg tataggaact ttagttcttg gaattgcctc tatcataatt
      121 ggtattacac tatttaagaa atttactttt atcaaagata ctactgcaat aattgttggc
      181 tcttttatat accaattcac tatatacttt gcaatgagtt taggaatgtt atcaactgac
      241 ttaaaactaa taactgccat agttataata gctttcctag ctactggtaa cttaaatatt
      301 tcactaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      361 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      421 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      481 agtaatggaa ctggtaaatc
//
LOCUS       DAEPOG010000361          496 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEPOG010000361 DAEPOG010000000
VERSION     DAEPOG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.367"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>496
                     /locus_tag="KR432_003720"
     CDS             <1..>496
                     /locus_tag="KR432_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HBH0642210.1"
                     /translation="LAEDEENNHINIEMQRAFENDYLERAEYYLSRVHGRKLEEGKEY
                     KEIGKTIGIHILNHVKYNHIEDYVNCLRLTMDEHPDIYSSKTALYFIELPKIHKSDYI
                     VNRIMLWGKLIDNPSSSDVRVIAKNDSIIKKALDRLKELGSNEEYLLNLKRGAYIMNK
                     SRNFK"
BASE COUNT          210 a           46 c           87 g          153 t
ORIGIN      
        1 tttggctgaa gatgaagaaa ataatcatat aaatattgag atgcaaagag cttttgagaa
       61 cgattatttg gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga
      121 aggtaaagag tataaagaaa ttggaaaaac tattggaata catatactaa atcatgtaaa
      181 atataatcac atagaagatt atgtaaattg cctgagatta acaatggacg agcatccaga
      241 tatttattct agtaaaactg cattatattt tatagagctt ccaaaaattc ataaatctga
      301 ctatatagta aatagaatta tgctatgggg aaaacttatt gataatcctt ctagctcaga
      361 tgtaagagtt atagctaaga atgattctat tattaaaaaa gcacttgata gattaaaaga
      421 attaggtagc aatgaagaat atttattaaa tctgaaaaga ggtgcttata tcatgaataa
      481 aagtagaaat tttaaa
//
LOCUS       DAEPOG010000362          491 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEPOG010000362 DAEPOG010000000
VERSION     DAEPOG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.368"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            168..>491
                     /locus_tag="KR432_003721"
     CDS             168..>491
                     /locus_tag="KR432_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH0642211.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKL"
BASE COUNT          223 a           31 c           71 g          166 t
ORIGIN      
        1 aagtcttttt aactttaaaa attaatgtta aaaaataaac ttatacatta atattaataa
       61 taattattta aatatcttaa gtaaatcagt cttacatata tttccgatag attatattga
      121 taaaaaacac taaaattaaa ttaataaaag tacttggagg gatggaaatg aaagaggaaa
      181 attttattaa tgttgaatac gaggataaac gtttttcaat taatagtaaa gaagttttgt
      241 acatagaaaa gcaagagtat gaagtaaaga tttatactga aagtggtaat tatgaagttg
      301 gagaaaatgc tccttgtata agaaaactat tgaaagaaga aaattttttc aaatgcagta
      361 atagttatat tgtaaatcta gacaagtgtg aattttatct cagcaataat tgtatttttg
      421 tgaataataa ttttataaga attttaggtt cagaaagaaa aaagaaatta aaaaagaaat
      481 tagaaaaatt a
//
LOCUS       DAEPOG010000363          491 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEPOG010000363 DAEPOG010000000
VERSION     DAEPOG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.369"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..212
                     /gene="srtB"
                     /locus_tag="KR432_003722"
     CDS             <1..212
                     /gene="srtB"
                     /locus_tag="KR432_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH0642212.1"
                     /translation="SAYIVESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSN
                     DKIVTLSTCTYEFDDARMVIHGRLI"
     gene            complement(372..446)
                     /locus_tag="KR432_003723"
     tRNA            complement(372..446)
                     /locus_tag="KR432_003723"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Thr,seq:tgt)
BASE COUNT          198 a           76 c           59 g          158 t
ORIGIN      
        1 tttctgctta tattgtagaa tctgattatg attatctaaa aacaaatttt aacaatgaat
       61 ctgattatca aaattacata aatgatatta cttctaaatc attgtataaa tctcctatta
      121 aggtaaatag caatgataaa atagttacac tttctacatg tacctatgaa tttgatgatg
      181 ccagaatggt gattcatggt agattgattt aaaactcaca gacacagttt aaaacaataa
      241 ttttaaactg tgtcatcttt tatacctata atttataaat taggccacta aaagtatgta
      301 atacaaaata acaaatacat caaacaataa aaaacaataa aaaaagacta tgctaaaaac
      361 atagtcttta ctggagctgg tgataggaat cgaacctaca acctgctgat tacaagtcag
      421 ctgctctacc gttgagccac accagcacac ctattctatt gtaaattagt aaaactaatt
      481 tttaaaagtt a
//
LOCUS       DAEPOG010000364          476 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEPOG010000364 DAEPOG010000000
VERSION     DAEPOG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.370"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(133..>476)
                     /locus_tag="KR432_003724"
     CDS             complement(133..>476)
                     /locus_tag="KR432_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0642213.1"
                     /translation="GEESRFSILYNSFGFENKDKNIKESSHGQNITFEYIAKQNPEVM
                     FVIDRGIATGSDVKESSTAKSVLNNDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLN
                     KMIDDASKAVN"
BASE COUNT          160 a           71 c           41 g          204 t
ORIGIN      
        1 tatcttatta taaataatat tattgtttaa actatatctt tattatgcaa aaaataaact
       61 aggagttctt aaaaactcct agtttatttg ataatcattt tacaaaaaat acattttgat
      121 tatatcggat tattaattta ccgcttttga agcatcatct atcattttat ttaaagatgt
      181 tagaccacca tcatttacat accatgttgg tgaatctaaa tatattatat tatcattctt
      241 atatgcatcc atagatttta ttatatcatt gttcaaaaca gattttgcag ttgagctttc
      301 ttttacatca ctaccagtag ctattcctct atctataacg aacataactt ctggattttg
      361 tttagctata tattcaaaag taatattttg tccatgactt gattctttta tatttttatc
      421 tttattttca aatccaaaac tattgtataa tatactaaat cttgattctt caccaa
//
LOCUS       DAEPOG010000365          475 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEPOG010000365 DAEPOG010000000
VERSION     DAEPOG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.371"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..393)
                     /locus_tag="KR432_003725"
     rRNA            complement(<1..393)
                     /locus_tag="KR432_003725"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          114 c           75 g          160 t
ORIGIN      
        1 aagtcttctc gtttaacata caggactatt accttctgtg gtgggtcttt ccaaacctct
       61 tcaattacga tacctcttcg ttatgatatg tccgcaaccc caataaagaa aactttattg
      121 gtttgggcta atccgcgttc gctcgccgct acttacggaa tcgaatttct ttctcttcct
      181 tcaggtactt agatgtttca gttccctgag ttcccctcat taagctatgt attcacttaa
      241 tgatacttag acattactct aagtgagttt cctcattcgg aaatcttcgg atcaaagttt
      301 acgtgcaact ccccgaagct tatcgcagct tatcacgtcc ttcatcggct cctagtgcca
      361 aggcatccgc cctgcaccct taataacttg accagttaaa aaggtttgat agattagaag
      421 ctatcaactt cgataatttt aaaaagttat cagctttata tattacaaat tagat
//
LOCUS       DAEPOG010000366          463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEPOG010000366 DAEPOG010000000
VERSION     DAEPOG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.372"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="KR432_003726"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="KR432_003726"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="KR432_003727"
     tRNA            301..375
                     /locus_tag="KR432_003727"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="KR432_003728"
     tRNA            380..454
                     /locus_tag="KR432_003728"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
BASE COUNT          145 a           83 c          108 g          127 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg gga
//
LOCUS       DAEPOG010000367          446 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEPOG010000367 DAEPOG010000000
VERSION     DAEPOG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.373"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>446
                     /locus_tag="KR432_003729"
     CDS             <1..>446
                     /locus_tag="KR432_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0642214.1"
                     /translation="VFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKI
                     YLNIIKNHQYQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEK
                     ISDDIDSEIFRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNI"
BASE COUNT          209 a           36 c           54 g          147 t
ORIGIN      
        1 tgtgttttta tttttatatc tatttattag ctacatcaag aagaacaaat ttttagataa
       61 gataaaaaat gatacattca aaattaatat gaatgatata gaattagcta gatgtaaaaa
      121 tgaagaaaaa atatacttga acattattaa aaatcatcaa tatcaatgta ataacataat
      181 aaataataat atcgagaaat ttgaagaaaa caaagagatt ttaaatatgt gggtacacga
      241 tgtaaagata cctatagcta taataaaact cattcttgaa caaaatgaaa atttaattaa
      301 tgaaaaaatt tcagatgaca tagatagtga aatatttaga attgaaaact ccgttgaaaa
      361 aattttatat ttatcaagac tcgaagattt tcataaggat tttttaattc aagaggtaaa
      421 tattgaaaaa atcattagaa atatta
//
LOCUS       DAEPOG010000368          445 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEPOG010000368 DAEPOG010000000
VERSION     DAEPOG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.374"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..314
                     /locus_tag="KR432_003730"
     CDS             <1..314
                     /locus_tag="KR432_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642215.1"
                     /translation="NDKNVFIGESENWKAKFVTTEGHSKLEIKYKNSKDVVEQKFNYS
                     YQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLYSNRESSYKLIISWNGNEETINLKK
                     K"
BASE COUNT          207 a           39 c           63 g          136 t
ORIGIN      
        1 aaaatgataa aaatgtcttt attggagaat cagaaaattg gaaagctaaa tttgtaacta
       61 ctgaaggaca ctcaaaatta gaaataaaat ataaaaatag taaagatgta gttgaacaaa
      121 aatttaatta ttcctatcaa tttatagatg gtgaagatgc agttgtagtt gtaaaaaatg
      181 aaattttaga agcaggtcaa gaaaaagcaa taaaaataca aagaccaggt ctttattcaa
      241 atagagaatc tagttacaag ctaattatat cttggaatgg aaatgaggaa actataaact
      301 tgaaaaagaa ataaccatat tatcaatttt taaaaaatta ttattcaaat tcttataaaa
      361 agagtgttta gaaaaatgaa acactctttt ttgtataagt ttttgaaaac tataaatgaa
      421 atatatcaaa atcgttcgaa aaatt
//
LOCUS       DAEPOG010000369          420 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEPOG010000369 DAEPOG010000000
VERSION     DAEPOG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.375"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>420
                     /locus_tag="KR432_003731"
     rRNA            <1..>420
                     /locus_tag="KR432_003731"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAEPOG010000370          417 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEPOG010000370 DAEPOG010000000
VERSION     DAEPOG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.376"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..359
                     /locus_tag="KR432_003732"
     CDS             <1..359
                     /locus_tag="KR432_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBH0642216.1"
                     /translation="AATNNRELNMEIGLYCRAKNKLVNVVDNIEISNFTVPSYIKRGD
                     LLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRKKVIKKYEDKSKRKDVL
                     NMLVTLDLEELKKFDI"
BASE COUNT          187 a           30 c           80 g          120 t
ORIGIN      
        1 ttgctgccac aaataataga gagttaaata tggaaatagg attatattgt agagcaaaaa
       61 ataagctagt gaatgtagtt gataatatag aaatatcgaa ctttactgtt ccatcatata
      121 taaaaagagg agatttactt ataagtgtgt ctactggggg caagagtcct tcattatcat
      181 caaagataaa aaaagaaatt gaagagagat atactgaaga ttatgaggag tatttaaatg
      241 tattaggaga tataagaaaa aaggttataa aaaaatacga agataaaagt aaaagaaaag
      301 atgtgcttaa tatgttggta acacttgacc tagaagaact taagaagttt gatatataaa
      361 aaataaaata aaaaatgtta aaaaggtgtt gacggattat ttttaagatg atatagt
//
LOCUS       DAEPOG010000371          401 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEPOG010000371 DAEPOG010000000
VERSION     DAEPOG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.377"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          179 a           23 c           60 g          139 t
ORIGIN      
        1 ataatttata aagtttatta atctaatatt gtgtgtgaat gttaagtggt caagtagtct
       61 aaaaaatgaa aaagatttaa aaaagtgttt taactaaaca cataagtgtt aaattcatgt
      121 ataagtatag gttagataca taagaaataa ttatgtaata tctatctttg taaaaaaata
      181 ttaaattttg taatttgtgg ataaatatgt ttataatgtg gataatattt aatgtaataa
      241 gttaaagcaa tatgaatcaa ctgtttataa tgtggataat attataaatg tatgaataat
      301 tgcagtattt aaaaacaaaa aaagttaaaa aagttgttga ctctaaaaga aaaagttggt
      361 atagtaatac ttgtcctcaa gaaaagggca aacaaataaa g
//
LOCUS       DAEPOG010000372          377 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEPOG010000372 DAEPOG010000000
VERSION     DAEPOG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.378"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..>377)
                     /locus_tag="KR432_003733"
     CDS             complement(<1..>377)
                     /locus_tag="KR432_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0642217.1"
                     /translation="IFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATI
                     IVYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLT
                     VGGPFSNFIEKSFIFVLIGLIIFS"
BASE COUNT          159 a           48 c           43 g          127 t
ORIGIN      
        1 gaaaatataa ttaatcctat aagtacaaaa atgaatgatt tttctatgaa attactaaat
       61 ggtcctccaa cagttaaaaa acctaaagaa attttctttt cgcttagcgg ataaaaaatt
      121 ggtattcctt gctttgtaaa taaatctcct aaaaacatat gacttgcata accaatcgta
      181 ccataaaaag ataaattatt aatattagtg attaattcta tctgttttag taaaaaatat
      241 actattatag ttgcaaatat gctatgagaa aaacttctgt gctttaacca gggaaaaatt
      301 gacaacatta gacaaaaaat tgtaaaatat atttcaactt ctatgatgta taagcatata
      361 gctaatagta taaatat
//
LOCUS       DAEPOG010000373          372 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEPOG010000373 DAEPOG010000000
VERSION     DAEPOG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.379"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          191 a           18 c           58 g          105 t
ORIGIN      
        1 gagctaactg aaagataagt aattaagaaa catttaatag aaaaagccaa ctgaaaaata
       61 aataattaag aaatgtttaa tggaagtgac taattgaaaa ataagtaatt aagaaatgct
      121 taatagaagt ggctaattga aaaataagta attaagaaat gtttaataga agtggctaat
      181 tgaaaaataa gtaattaaga aatgtttaat agaagtggct aattgaaaaa taagtaattg
      241 agaaacattt aatagaagaa gctagctgaa agataagtaa ttaagagata tttaatgtaa
      301 acagataatt taaaaatacc taattaagaa tagatattaa aaaatataat ttcaatataa
      361 aaataagtgt at
//
LOCUS       DAEPOG010000374          367 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEPOG010000374 DAEPOG010000000
VERSION     DAEPOG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.380"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          136 a           46 c           25 g          160 t
ORIGIN      
        1 ttctaaattt aggtatgctt tactatataa aaatgatttt ttatcttaat tttctaaata
       61 taagtgaatg ctatcatttt aagtatctta taagcaatgt aactatatat ctctaataac
      121 tactttttta acatgcttaa actatttata agctattata tttatgtatt aacctaataa
      181 ctttttcact aaaaaataac aatctattca aagataaact atagtacttc ttctactttg
      241 tttcaataaa atactaaata aatttcgtac atccacaatt tttcaggcta cactataaat
      301 atacaatatt tatcttagtt ataagtttaa gaatattttt agtcttattt tgtcgaatat
      361 attttta
//
LOCUS       DAEPOG010000375          364 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEPOG010000375 DAEPOG010000000
VERSION     DAEPOG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.381"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KR432_003734"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KR432_003734"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="KR432_003735"
     tRNA            291..362
                     /locus_tag="KR432_003735"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAEPOG010000376          363 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEPOG010000376 DAEPOG010000000
VERSION     DAEPOG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.382"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          145 a           58 c           29 g          131 t
ORIGIN      
        1 ataaataata tatcgttttc ataaattata tctctcaaat tgctgtaaat ctttgttttt
       61 gttaaattta ttgattctaa taaagataat tgattccttc cataaaagcc tgaaacacaa
      121 taaccttttg ataaaaaata atttgccaaa gatgttccaa cttttccaga accaataaag
      181 cctattttaa tagtatcacc tccactttgt gatacaacat cttaatttct aatctcattc
      241 ctgaggatat gagttacata agacaaaata attaaaaaca tttaaagtat agttcttata
      301 acaagatacc atccataaat atcttaccat tttcataaaa aattgacaat taattctata
      361 aaa
//
LOCUS       DAEPOG010000377          362 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEPOG010000377 DAEPOG010000000
VERSION     DAEPOG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.383"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(97..213)
                     /gene="rrf"
                     /locus_tag="KR432_003736"
     rRNA            complement(97..213)
                     /gene="rrf"
                     /locus_tag="KR432_003736"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(222..295)
                     /locus_tag="KR432_003737"
     tRNA            complement(222..295)
                     /locus_tag="KR432_003737"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Gly,seq:tcc)
BASE COUNT          127 a           75 c           57 g          103 t
ORIGIN      
        1 tcactatatc atttattgcc aaaaaaatct ataaaagaca aataaaaaca tataacaatt
       61 gtaggtttat taaatttaat acaacaaaaa aagattacgt ggctacgtcc tactctccca
      121 ggcagtttcc caccaagtac catcagcact agagagctta acttctgtgt tcggaatggg
      181 aacaggtgta tcctctctgc tattgtaacc acataatctt ttggagcggg tgaaggggat
      241 cgaaccctca cagctggctt ggaaggccag aactctacca ttgagctaca cccgcattaa
      301 atttttataa attagtaaaa ctaatttttt agagttagca ccctaaaaac tacatatata
      361 tc
//
LOCUS       DAEPOG010000378          361 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEPOG010000378 DAEPOG010000000
VERSION     DAEPOG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.384"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(87..162)
                     /locus_tag="KR432_003738"
     tRNA            complement(87..162)
                     /locus_tag="KR432_003738"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Gln,seq:ttg)
     gene            complement(170..246)
                     /locus_tag="KR432_003739"
     tRNA            complement(170..246)
                     /locus_tag="KR432_003739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Arg,seq:tct)
     gene            complement(276..359)
                     /locus_tag="KR432_003740"
     tRNA            complement(276..359)
                     /locus_tag="KR432_003740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:tag)
BASE COUNT          111 a           87 c           73 g           90 t
ORIGIN      
        1 attcggatac ttaaacttat tctaaaattg aacctaacta tatttaaaat aaaaatagtt
       61 caaatatagc ttagtaatac atcatttggc tggggatgca ggactcgaac ctacgcatac
      121 atgagtcaaa gtcatgtgcc ttaccgactt ggctaatccc caatatatgt ggtgagcgca
      181 cagggattcg aaccccggac acacgcctta gaagggcgtt gctctatcca gctgagctat
      241 gcacccacaa tttatatatg tatatcaaac aaaaatggtg cggatgaagg gagtcgaacc
      301 cccacgccaa tggcgctaga tcctaagtct agtgcgtctg ccaattccgc cacatccgcg
      361 c
//
LOCUS       DAEPOG010000379          361 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEPOG010000379 DAEPOG010000000
VERSION     DAEPOG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.385"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            201..341
                     /locus_tag="KR432_003741"
     CDS             201..341
                     /locus_tag="KR432_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642218.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
BASE COUNT          157 a           22 c           65 g          117 t
ORIGIN      
        1 tatataaaaa agataataat agttagactt tttatatgaa ttttaatagg taaaatatcc
       61 tattgttttg tagatatatc ctaattatgt caaaaaataa aaaaattttt atttattaga
      121 gtttttggaa ataataataa cagatattat aattaattaa cagtttttgt aagtatttga
      181 caatagagag ggtgtaacaa atgggaataa tagagtcagc atcaaaattg gctgagatgg
      241 ttcatttatt ggctgttgaa aaggggataa ctgatataga agcatgggat gaagctgtga
      301 aagaatacag caaaatttat gaagaaagaa gaaatgaata gaatgataaa aaatgaaaac
      361 t
//
LOCUS       DAEPOG010000380          347 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEPOG010000380 DAEPOG010000000
VERSION     DAEPOG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.386"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>347
                     /locus_tag="KR432_003742"
     CDS             <1..>347
                     /locus_tag="KR432_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642219.1"
                     /translation="RKNLVHYKNNRVAIMDLNSYLIKKSDNLSKKISINERSYEIFKD
                     EKFISSKVGKEVLKNLKIDLIKDLNVYKTPEPFIYLSINRISPQKILIIENKDTYITI
                     TKMLLEGKEILKN"
BASE COUNT          177 a           35 c           40 g           95 t
ORIGIN      
        1 atagaaaaaa cttagtacac tataaaaata atagagttgc aataatggat ttaaatagtt
       61 atcttattaa aaaatctgat aatctatcaa agaaaatatc gattaacgaa agatcttatg
      121 aaatatttaa ggatgagaaa ttcataagta gtaaagtagg aaaagaagta ctaaaaaact
      181 taaaaataga tctaataaag gatttaaacg tatataagac accagagcca ttcatatatt
      241 tatcaataaa tcgtatatct ccacaaaaaa tattaattat agaaaataaa gacacttaca
      301 taacaataac aaaaatgcta ttagaaggaa aagaaatact taaaaat
//
LOCUS       DAEPOG010000381          343 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEPOG010000381 DAEPOG010000000
VERSION     DAEPOG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.387"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>343
                     /locus_tag="KR432_003743"
     rRNA            <1..>343
                     /locus_tag="KR432_003743"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAEPOG010000382          341 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEPOG010000382 DAEPOG010000000
VERSION     DAEPOG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.388"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            12..88
                     /locus_tag="KR432_003744"
     tRNA            12..88
                     /locus_tag="KR432_003744"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Pro,seq:tgg)
     gene            96..172
                     /locus_tag="KR432_003745"
     tRNA            96..172
                     /locus_tag="KR432_003745"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:His,seq:gtg)
     gene            181..256
                     /locus_tag="KR432_003746"
     tRNA            181..256
                     /locus_tag="KR432_003746"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Lys,seq:ttt)
     gene            264..337
                     /locus_tag="KR432_003747"
     tRNA            264..337
                     /locus_tag="KR432_003747"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Cys,seq:gca)
BASE COUNT           78 a           79 c           96 g           88 t
ORIGIN      
        1 taatataaat tcgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
       61 gggggttcga gtcccttcac ctcgaccaat ataaggtggg tatagctcag ttggttagag
      121 cgccagattg tggctctgga ggtcgtgagt tcgactctca ttatccaccc caattcatat
      181 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
      241 cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac
      301 tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa t
//
LOCUS       DAEPOG010000383          339 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEPOG010000383 DAEPOG010000000
VERSION     DAEPOG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.389"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          130 a           36 c           33 g          140 t
ORIGIN      
        1 taatacatat taactgtaaa attaaattta tagagttata tttaacattt aatgaactga
       61 aaaataaatt agcaaataat gaaaaaacaa cagtaataaa tgagaataac aaagcattct
      121 caggattagt ggatttttct aattgagctt taatcctagt ttgttcaatt tcgatatttc
      181 taatattatt agtattatca atttatttgt aataattatc aatttttgcg aaatattttt
      241 gataatttat agaactttat atattttcct taaagtattt ttagccctta ttgtttggtt
      301 tccaatttta aaactcataa actcccaata aattcatgt
//
LOCUS       DAEPOG010000384          327 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEPOG010000384 DAEPOG010000000
VERSION     DAEPOG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.390"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>327
                     /locus_tag="KR432_003748"
     CDS             <1..>327
                     /locus_tag="KR432_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0642220.1"
                     /translation="YSLNIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREF
                     MKYISAIGDIAVEEYLIKKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKN
                     GNKIDS"
BASE COUNT          139 a           19 c           59 g          110 t
ORIGIN      
        1 tatattcact gaacatattt gctaaaaaag agatttttat gagagaaaca gtatttacta
       61 gggtgagaaa ttatatagaa gaaaatgatt ttataaatgt agaaggtttt attagattta
      121 gaatgagaga atttatgaaa tatatttctg ctataggtga tattgcagtt gaggagtatt
      181 taataaagaa agaccaagat gaatttataa gagttttaaa atactttata gatactcaag
      241 aagaaaagat tgatttgctt aaagttcata taatggaaga taatacattt gttttatacg
      301 ataaaaatgg aaataagata gacagta
//
LOCUS       DAEPOG010000385          322 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEPOG010000385 DAEPOG010000000
VERSION     DAEPOG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.391"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>322
                     /locus_tag="KR432_003749"
     CDS             <1..>322
                     /locus_tag="KR432_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0642221.1"
                     /translation="VMDSIEAPRYKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIG
                     LGLRISEVLGLTWDNIDFEENTITVNKITSRINGSVILKEPKTESSVRKISSPKELMS
                     LLKEY"
BASE COUNT          134 a           33 c           52 g          103 t
ORIGIN      
        1 tgttatggat agtatagagg ctccaagata taaaaaattt aaaaatgaga tttatgatag
       61 agaacaaatg ataaaattat tagatttagc aaaaaataca gaaatggaat tacctattaa
      121 tttggctatt ggattaggtc ttagaatttc agaagtttta ggtctaactt gggataatat
      181 tgattttgaa gaaaatacaa taacagtaaa taagataact tcccgaatta atggttctgt
      241 tattctcaaa gagccaaaga cagaaagctc tgttagaaaa atatcttctc ccaaagaatt
      301 aatgtcttta ttaaaagaat at
//
LOCUS       DAEPOG010000386          306 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEPOG010000386 DAEPOG010000000
VERSION     DAEPOG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.392"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..291)
                     /locus_tag="KR432_003750"
     CDS             complement(<1..291)
                     /locus_tag="KR432_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0642222.1"
                     /translation="MSADGSYTMGASIVAFSLTNGISPVVATLMAILCGCTAGLVTGI
                     LHIKFKISNLLSGILVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLAL"
BASE COUNT          123 a           52 c           49 g           82 t
ORIGIN      
        1 taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc taaatagagg
       61 aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca taactaaaat
      121 accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa caagaccagc
      181 agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac cattagtaag
      241 agaaaatgct acaatagaag cacccattgt atagctacca tcagcagaca tgtctggaaa
      301 atctag
//
LOCUS       DAEPOG010000387          296 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEPOG010000387 DAEPOG010000000
VERSION     DAEPOG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.393"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          117 a           30 c           27 g          122 t
ORIGIN      
        1 gtcgatttat taaagttatc tatgtactat attaaacaat taagtagtta ttaattgcac
       61 ttattagttt attaaaaaac tatgtatatt actttgcagt aagactttgg aactaaatct
      121 ataatagcaa atttaaattt attaaataga ttaccttata acatttcata taaaatatat
      181 tgtgaatctg agaaaattac tatttttctt tgagtatttt ttataagcag ttctaaaaga
      241 ttcaatttca tacctattac ttgaagcatt cattaaaata attctaattt aaaaaa
//
LOCUS       DAEPOG010000388          279 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEPOG010000388 DAEPOG010000000
VERSION     DAEPOG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.394"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          128 a           28 c           18 g          105 t
ORIGIN      
        1 ccttctttta ttaaaatatt atttattttt tcttttagtt ttattccaaa acaaacatca
       61 ttatcaaaaa ttaatatatc cactaaatac cctcctataa aaaattcaaa agtaaaaaaa
      121 ctaaaagtat tttataaaaa atacgtaaaa taacactagg agataaataa aattaaggat
      181 agttagaatt aaggtatatt tatgcttgtt tatatagtat atcttatact tatagtttac
      241 aataataaag taaattaaat caatatatta ttataaaaa
//
LOCUS       DAEPOG010000389          269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEPOG010000389 DAEPOG010000000
VERSION     DAEPOG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.395"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          119 a           21 c           22 g          107 t
ORIGIN      
        1 atttttatta attataatat ttgaataatc tttttaatca tataaaatgc atatacttgt
       61 aatatttaaa ggtgatatta atattattag tgtatattaa tattcccttg aaattaaaaa
      121 aacatagata tttcagtaat ctaaaatagt ctacaaaaat aacagtttta gcaaaattaa
      181 taagaataca tatagattta aacctaaaag tatgaacatg tctgtttttt acaagattaa
      241 aatttatatt atatgataaa attaactta
//
LOCUS       DAEPOG010000390          265 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEPOG010000390 DAEPOG010000000
VERSION     DAEPOG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.397"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>265
                     /locus_tag="KR432_003751"
     CDS             <1..>265
                     /locus_tag="KR432_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0642223.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIV"
BASE COUNT          111 a           29 c           50 g           75 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttg
//
LOCUS       DAEPOG010000391          251 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEPOG010000391 DAEPOG010000000
VERSION     DAEPOG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.398"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT           94 a           22 c           42 g           93 t
ORIGIN      
        1 ttccaaaatt tacaataaat aaatctatat aaatcatata aattatttat gtcatgtgat
       61 agaatattaa tgaataaacg tttaagtgag gatgaataat atttttagta agctacttct
      121 gataaattta tttataaatt tatcgtgtgt tctgcagggt ggtttattta ttatgcaaag
      181 gggtgcgttt tgatgtatgg tgaaaacaaa aagttaagta ttgattaaaa aatagctacg
      241 cacactcgtg a
//
LOCUS       DAEPOG010000392          213 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEPOG010000392 DAEPOG010000000
VERSION     DAEPOG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.400"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          107 a           21 c           14 g           71 t
ORIGIN      
        1 gaaaagaaaa tattaaaatt ataatataaa aataaaattt ttcagacaat tcaaacaaaa
       61 ataatcacct tttaataaat aattatatgc caaaataatc caatttattt tctattatac
      121 ctatatgaaa tatataaatc aatagttaag acaatgaata tgctacattc tataattata
      181 gtagcagaaa attttacaaa attaaagtag ata
//
LOCUS       DAEPOG010000393          213 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEPOG010000393 DAEPOG010000000
VERSION     DAEPOG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.401"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT           89 a           19 c           16 g           89 t
ORIGIN      
        1 gtgtataatt tgattattat atactgcata caaaaatata tttgagcaaa taagtaaagt
       61 aataaaaaaa tcaagatttc atatctaaaa actattctga acattaattt aatttatatt
      121 ttcataggaa atattttata taatacactt aacaaatata atttaaattt ttctaattat
      181 ttactgtcga ttaatagatt tttttcttgc ata
//
LOCUS       DAEPOG010000394          209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEPOG010000394 DAEPOG010000000
VERSION     DAEPOG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.402"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..>209
                     /locus_tag="KR432_003752"
     CDS             78..>209
                     /locus_tag="KR432_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0642224.1"
                     /translation="MISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDE
                     "
BASE COUNT           97 a           21 c           35 g           56 t
ORIGIN      
        1 atagtattac agacaacaca tataaatggt taaagagtga ggatttacaa aatgcttatt
       61 ttataggtgg acctcaaatg atatcaacaa atgttataaa taaggtaaat gatataacta
      121 aagataatgt tactaataat agagtatatg gagcagatag acacgaaaca aatgcaaacg
      181 taataaaaaa attctataca gatgatgag
//
LOCUS       DAEPOG010000395          209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEPOG010000395 DAEPOG010000000
VERSION     DAEPOG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.403"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..>209
                     /locus_tag="KR432_003753"
     CDS             78..>209
                     /locus_tag="KR432_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0642225.1"
                     /translation="MISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTEDE
                     "
BASE COUNT           94 a           20 c           39 g           56 t
ORIGIN      
        1 atagtattac agacaataca tataaatggt taaagagtga ggatttacaa aatgcttatt
       61 ttataggtgg tcctcaaatg atatcaacaa atgttataaa taaggtaaat ggaataacta
      121 aggatagtgt tactaataat agagtatacg gagcagatag acacgaaaca aatgcaaatg
      181 taataaaaaa attctataca gaggatgag
//
LOCUS       DAEPOG010000396          206 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEPOG010000396 DAEPOG010000000
VERSION     DAEPOG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.denovo.404"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..167)
                     /locus_tag="KR432_003754"
     rRNA            complement(<1..167)
                     /locus_tag="KR432_003754"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAEPOG010000397         4867 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.226, whole genome shotgun sequence.
ACCESSION   DAEPOG010000397 DAEPOG010000000
VERSION     DAEPOG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.226"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1070
                     /locus_tag="KR432_003755"
     CDS             <1..1070
                     /locus_tag="KR432_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0642226.1"
                     /translation="GNSTEYDVSLQSAFAVLENLNTEKYDIIPIGITREGNWYHYIGD
                     YNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNSAIESCENDIKY
                     TKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDKSHKLVHSEGIK
                     VPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEH
                     DNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPAR
                     IDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIPGFTSHSRYPNM
                     MKGIGLSFEEILDKIIEVYIK"
     gene            1067..1873
                     /locus_tag="KR432_003756"
     CDS             1067..1873
                     /locus_tag="KR432_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0642227.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            1891..4029
                     /gene="vanT"
                     /locus_tag="KR432_003757"
     CDS             1891..4029
                     /gene="vanT"
                     /locus_tag="KR432_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0642228.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            4343..>4867
                     /locus_tag="KR432_003758"
     CDS             4343..>4867
                     /locus_tag="KR432_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0642229.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSW"
BASE COUNT         1741 a          555 c          850 g         1721 t
ORIGIN      
        1 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
       61 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
      121 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
      181 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
      241 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
      301 ttaaatacac taaacttgat tttgtatttc ctgtattgca tggaaaaaat ggagaagatg
      361 gaacagtgca ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt
      421 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
      481 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
      541 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
      601 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
      661 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
      721 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
      781 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
      841 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
      901 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
      961 atacaattcc tggctttact tctcatagtc gttatccgaa tatgatgaaa ggtattggac
     1021 tgtcttttga agaaatattg gataagataa ttgaggtgta cataaaatga tgaataatat
     1081 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     1141 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     1201 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     1261 aaatatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     1321 tggatatcgt actgcagagg aacaacaaga tatatattca agctctctgc gtgacaatgg
     1381 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     1441 tgctattgat ttagggttga agaaagaggt tatagatttt atctgccctg aatttcctta
     1501 taatggtatt tgtaatgagt tccgtaaggc agcactacat tatggtttta tagagcgtta
     1561 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     1621 cggatatcca cattctgaga ttatcaataa aatgaatctc gcccttgaag aatatataga
     1681 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     1741 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     1801 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     1861 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     1921 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     1981 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     2041 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     2101 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     2161 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     2221 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     2281 gcaagtattg catactttct tgtaaaaaaa ctaggtttaa agtactcatt agtaatcact
     2341 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     2401 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     2461 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     2521 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     2581 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     2641 ccatgtatgt atttcttatt tacagcctta acatttttaa aaggttctcg agctaggtta
     2701 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     2761 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     2821 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     2881 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     2941 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     3001 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     3061 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     3121 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     3181 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     3241 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     3301 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     3361 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     3421 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     3481 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     3541 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     3601 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     3661 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     3721 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     3781 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     3841 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     3901 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
     3961 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
     4021 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
     4081 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
     4141 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
     4201 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
     4261 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
     4321 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ttacttgatt tgactcaaaa
     4381 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
     4441 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
     4501 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
     4561 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
     4621 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
     4681 gcctggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
     4741 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
     4801 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
     4861 gtcatgg
//
LOCUS       DAEPOG010000398         4867 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.227, whole genome shotgun sequence.
ACCESSION   DAEPOG010000398 DAEPOG010000000
VERSION     DAEPOG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.227"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1070
                     /locus_tag="KR432_003759"
     CDS             <1..1070
                     /locus_tag="KR432_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0642230.1"
                     /translation="GNSTEYDVSLQSAFAVLENLNTEKYDIIPIGITREGNWYHYIGD
                     YNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNSAIESCENDIKY
                     TKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDKSHKLVHSEGIK
                     VPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEELQVAVDLAFEH
                     DNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYTLKSSKIHMPAR
                     IDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIPGFTSHSRYPNM
                     MKGIGLSFEEILDKIIEVYIK"
     gene            1067..1873
                     /locus_tag="KR432_003760"
     CDS             1067..1873
                     /locus_tag="KR432_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0642231.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            1891..4029
                     /gene="vanT"
                     /locus_tag="KR432_003761"
     CDS             1891..4029
                     /gene="vanT"
                     /locus_tag="KR432_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0642232.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHNVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            4343..>4867
                     /locus_tag="KR432_003762"
     CDS             4343..>4867
                     /locus_tag="KR432_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0642233.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSW"
BASE COUNT         1741 a          555 c          849 g         1722 t
ORIGIN      
        1 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
       61 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
      121 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
      181 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
      241 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
      301 ttaaatacac taaacttgat tttgtatttc ctgtattgca tggaaaaaat ggagaagatg
      361 gaacagtgca ggggttattt gaattggcag gtatacaggt tattggatgt ggtactcttt
      421 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
      481 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
      541 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
      601 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
      661 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
      721 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
      781 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
      841 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
      901 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
      961 atacaattcc tggctttact tctcatagtc gttatccgaa tatgatgaaa ggtattggac
     1021 tgtcttttga agaaatattg gataagataa ttgaggtgta cataaaatga tgaataatat
     1081 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     1141 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     1201 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     1261 aaatatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     1321 tggatatcgt actgcagagg aacaacaaga tatatattca agctctctgc gtgacaatgg
     1381 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     1441 tgctattgat ttagggttga agaaagaggt tatagatttt atctgccctg aatttcctta
     1501 taatggtatt tgtaatgagt tccgtaaggc agcactacat tatggtttta tagagcgtta
     1561 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     1621 cggatatcca cattctgaga ttatcaataa aatgaatctc gcccttgaag aatatataga
     1681 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     1741 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     1801 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     1861 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     1921 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     1981 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     2041 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     2101 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     2161 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     2221 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     2281 gcaagtattg catactttct tgtaaaaaaa ctaggtttaa agtactcatt agtaatcact
     2341 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     2401 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     2461 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     2521 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     2581 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     2641 ccatgtatgt atttcttatt tacagcctta acatttttaa aaggttctcg agctaggtta
     2701 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     2761 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     2821 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     2881 ctaaaaaaag acttgaaagg agaacataat gtaaatacca atagagcttg gattgaaatt
     2941 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     3001 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     3061 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     3121 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     3181 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     3241 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     3301 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     3361 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     3421 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     3481 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     3541 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     3601 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     3661 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     3721 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     3781 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     3841 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     3901 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
     3961 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
     4021 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
     4081 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
     4141 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
     4201 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
     4261 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
     4321 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ttacttgatt tgactcaaaa
     4381 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
     4441 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
     4501 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
     4561 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
     4621 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
     4681 gcctggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
     4741 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
     4801 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
     4861 gtcatgg
//
LOCUS       DAEPOG010000399         3095 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.273, whole genome shotgun sequence.
ACCESSION   DAEPOG010000399 DAEPOG010000000
VERSION     DAEPOG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.273"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            27..965
                     /gene="blaCDD"
                     /locus_tag="KR432_003763"
     CDS             27..965
                     /gene="blaCDD"
                     /locus_tag="KR432_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0642234.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1309..1992
                     /locus_tag="KR432_003764"
     CDS             1309..1992
                     /locus_tag="KR432_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0642235.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            1989..>3095
                     /locus_tag="KR432_003765"
     CDS             1989..>3095
                     /locus_tag="KR432_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0642236.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVN"
BASE COUNT         1206 a          347 c          538 g         1004 t
ORIGIN      
        1 cttatgtaga ggagatggta taaagtatga aaagaaaaaa taattttatt tggatagtta
       61 ttcttctagt tgttgtagct atgggagtat attatgtagg aagaaagcat aacaacacaa
      121 aacaaaaaca tgataagaat catataaact ctgaattaaa taaatccaaa gttgaaaaca
      181 atgataaaaa caaaaaagaa aaaattaata tggttgacta tagtgactgt tttgaaggta
      241 ttagtggagg agctattttt tataatacta aaaataaaga atataatata tataacaaag
      301 aattaattga aacaagaagg tcaccatgtt ctacttttaa aatagtttca actttaattg
      361 gtttagaaaa aggggtaata aactcaaaag aatcagttat gggatatgat ggtacagagt
      421 atccaaataa aaattggaat aaaaatctta gtttagaaga ggcatttaag gagtcttgtg
      481 tttggtacta caaaaaattg atagataaag ttgatgctaa atctgtccaa aacattttag
      541 atgatttaaa gtatgggaat tgcgatatta gcgaatggga aggagattta aaaaatggaa
      601 agggacattt aaatggattt tggttagaat cttcattaca aatatctcca aaagaacaag
      661 tgcaaacaat ggcaaaaata tttgagggag atacaaactt taaaaaagag catattaata
      721 ttttaagaga tattatgaaa atagatgtaa atgataaaaa cataaatgta tatggaaaaa
      781 ctggaacagg atttgatgaa aaaaataaac gtgtggatgc atggtttgtt ggtatgctag
      841 agcgtgaagg tgacacctat tattttgcta ttaaatcaga tgattccaat aaagaaatta
      901 cagggccaaa ggtaaaggaa attgcaatta atataattaa gaaatattat agtgtaaggg
      961 aataaactta attttatttg taaatattat ttacctatag tctgtaatat ttattaaata
     1021 attttactaa attgagtcta tatagagcaa aactccttta gtacataaaa tacttgctat
     1081 tgattattaa attgaatacc tatagattta aagttaatac cttttaaaga ctatgtaata
     1141 tattaaaagt ataaataaaa tatattgcat agtttttgtt ttatattata attttaaata
     1201 atgtgcaaaa actaaacaat atttttaata gtaatttata tttgtattca tgtcactata
     1261 atgtcatttt aggttgctaa aatttaacca taaaggagat gattgtatat ggaaatatta
     1321 aaatgtgaaa atctcacaaa aatatatggt tcaaatcaaa ccagagtgac agcccttaat
     1381 aatgtaaatt tatcagttca aaaaggtgat tttgtatcca ttgtaggagc atctggttct
     1441 ggcaaatcta cattgttaca tctgcttggg ggagtagata gacctacatc tggaaagatt
     1501 tatgttgaag atacagaaat atcaagttta aaggaagaag ctcttgcagt ttttagacgt
     1561 agaaaagtag gtcttattta tcaattttat aatcttatac ctacccttga tgttagaaaa
     1621 aatatattat tacccatgtt acttgataaa agaaaggtgg atgaggatag attctctgag
     1681 attgtatcta tattgggatt atctgataga ttaaaccatc ttccaagtca actttcaggt
     1741 ggacaacaac aaagggtatc tatagctaga agtttaatct atagaccagc tattttatta
     1801 gcagatgagc caactggtaa tcttgataga aaaaattcag aagaaattgt agacttgtta
     1861 aacttatcaa ataaacgatt taatcagaca atacttctta ttactcatga tgagaaaata
     1921 gcactagagg caaatcgtat tgtaactatg gaagatggag tgattgtctc tgaaaaggtg
     1981 gtgaagaaat gaatattctt caagaataca ccttagactt tgtacgacgc aataaacgta
     2041 gtagtatagc tataatgtta gctatattac ttactacaac catgatgtct tctttgtgtg
     2101 gactgttgta taccatgtgg acagatttta taagactttc aattgagaaa gaaggtaact
     2161 ggcatggtga attgtttgac tatacttatg gaagtgactt accattaatt gaaaattttt
     2221 cttcagttga tgatgtaatg gtaaaaggac aatggtatgt aggcaaaatt aatgatgaga
     2281 aaagagatta tataatttat agaaatgcta catcagagta ttggaattct atgccagaaa
     2341 aggatactat aatagagggt cgtcgaccaa ataatgctaa tgaaattgca ttatcaaaac
     2401 aatattttga gaataatcca agtgtaaaaa ttggggataa aattactctt ccaataggag
     2461 atagaatcct aaatggaaag cctcttgaac ccatagctca aaaatcgaaa ggggaaagtt
     2521 ttaaaaaaat ttcagagact acattgacaa tagtaggaaa ggtagatgtt tccacttcat
     2581 cagtaatacc tgcatataca gctcttggat atcttgatag aacaagtata aaacctaatg
     2641 atagtataac tgtgtacttg cgatttaaaa atataagaga cacatacaag gaacttccaa
     2701 aacttgctaa atcattgggg tggaagacta atgaatatgg aaaatacaat cttaaatata
     2761 atagtaatta tcttttaaaa atgttagtct tttccccaga acagaaagca tctatgagca
     2821 gtatatcaaa attttcaact cctattatgt atcttacaat agcaattttt acagtagctg
     2881 tgtttgtaat ggtgatttac aacgcgtttt cactatctgc aaatgctaga cttactcaat
     2941 tagggatatt atctagtgtt ggtgcatcac caaaacagat taaaatgtct gtagtgtttg
     3001 aaggtttttt gcttacaatt ataccattgc caataggttt atttttagga tggttattgt
     3061 gtaataggct tatagtttat attaattcag ttaat
//
LOCUS       DAEPOG010000400         2478 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.290, whole genome shotgun sequence.
ACCESSION   DAEPOG010000400 DAEPOG010000000
VERSION     DAEPOG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.290"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            359..1060
                     /gene="vanR"
                     /locus_tag="KR432_003766"
     CDS             359..1060
                     /gene="vanR"
                     /locus_tag="KR432_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0642237.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1110..2192
                     /locus_tag="KR432_003767"
     CDS             1110..2192
                     /locus_tag="KR432_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0642238.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
BASE COUNT          932 a          263 c          428 g          855 t
ORIGIN      
        1 ttttgaattt ttatataaaa taacatggaa attttagtaa tgtctgtggt tacatacata
       61 ggtttaattg ttttttatat ggattgtctc tggtataata aattgaattg tgtttgactg
      121 atagatattt ttattgaaag ttaatacctg ttatgcgata ataaggacgt gttaggggag
      181 tttttataat aacaattaat ctcagttttt gtgttccaat agtagcatca acacattctg
      241 atgtagtagt tcttttctta taaaaattag ttacaaatat gcttgtaatt atttgatatg
      301 taaatttacc aatattataa atgatgacct cgataataag gagagtgaaa aataatctat
      361 gaacgaaaat attttgattg tggatgataa aaaagaaata gcagatttaa ttgaagtata
      421 tttaaaaaat gaaggtttta cagtatacaa gttttataca ggtaaggaag ctctagaatg
      481 tattgaatct aaagatttgg atatggctat tttagatatt atgcttcctg atattgatgg
      541 atttcatatt tgccagaaaa ttcgagaaaa atactattat cctatcatta tgttaacagc
      601 taaaattgaa gatatagaca agataatggg tttaaccata ggtgctgatg attatataac
      661 aaagccattt aaccctttgg aagttgttgc aagagtaaag acacaattaa gaagatatat
      721 gcgatataat aattcttatg aacaacagtc gataattgtt aatgagtatg atattaaagg
      781 attgattatc aataaagaaa cacataaatg tagtttattt gggaaagaag tagcattaac
      841 accgattgaa ttttctattc tttggtattt atgtgaacat caaggaaaag tggttccttc
      901 tgaagaattg tttgaagctg tatggggaga aaaatatctt gataataata acacagttat
      961 ggctcatata gggcgattac gtgaaaaaat tcatgaacct tctaaaaacc cgaaatttat
     1021 aaaaactgta tggggagtag gttatacaat tgaaaagtaa aaatattaaa gtcaatcctg
     1081 actttactca agtgagaaat aaaattttta tgagaacagc tttaatggca tttatagcca
     1141 ttattttcat atggatttta tattctttta tattgtatgg taagttttct atctggatag
     1201 tatcattctt tgaaaatgct tttcgtatgg aatataaagc tgcattgaat ttatatcagc
     1261 agattttccg taatagcatg gagctcttca tgatactagc tatggtgatt gtgtttttta
     1321 taatattccg tgtttattta aattggttta caaaatattt tagtgaaata aatcaaggta
     1381 ttgactctct tatcaaagaa gatgttggag aagtggcttt atctccagag ttattggcaa
     1441 tagaaaagaa aataaactca attaaacata tacttgaaca acgtaaattt gagacacaaa
     1501 tggcagaaca acgtaagaat gaacttattg tttatcttgc acatgattta aaaacaccac
     1561 ttacatcagt tatagggtat ctgacactac ttcgagatga aagtcagatt tctgaagaac
     1621 tgcgaaaaaa atatttatct atttcgttgg ataaggcaga gcgtcttgaa gatttaatta
     1681 atgaattctt tgaaattaca aggtttaatt tatcaaatat atcacttgaa tacagtacag
     1741 taagcttaac tcgtatgctt gaacaactaa catatgagtt taaacctact ttgatgagta
     1801 agaatttaaa atatacactg aatgttacac caaatatgat gataaggtgt gatgttaata
     1861 aaatgcaacg tgtatttgat aacttattac gaaatgctgt ttattatagt tttgaagata
     1921 ctactattga aataacagct gtacaggagg atgattatat aaagcttaaa tttataaatc
     1981 atagcaatac gattccagag gaaaaacttg agcgtatttt tgaacaattt tacaggttgg
     2041 atacatctcg taattcaaac agtggtagag ctgggttggg acttgctata gctaaagaaa
     2101 tagtggagct tcataacggg acaataacag ctcatagcga gaacaatata attaccttta
     2161 aggttattat tccgattgta ggaaatttgt aagaatttaa gtataaaaaa ataggaaatt
     2221 ctttaggata aataaagagc actcttgtta ttgatataat tactgtatca aaacaagagt
     2281 gcttttattt tgatacagta attatatcaa tagaaaaatg tgaaaatata ttatgtggaa
     2341 cagatgcaat tgtaagtgaa ggttgattgg atttgaaata gaaaaagtat tttaacatgt
     2401 ttctgtagat tgtataaaaa taaataaatc aacaaaggag aatcaaagat gaataataaa
     2461 aaaattgcag ttatattt
//
LOCUS       DAEPOG010000401         1092 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.330, whole genome shotgun sequence.
ACCESSION   DAEPOG010000401 DAEPOG010000000
VERSION     DAEPOG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.330"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..776
                     /gene="vanR"
                     /locus_tag="KR432_003768"
     CDS             78..776
                     /gene="vanR"
                     /locus_tag="KR432_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0642239.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            812..>1092
                     /locus_tag="KR432_003769"
     CDS             812..>1092
                     /locus_tag="KR432_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0642240.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKP"
BASE COUNT          409 a          120 c          176 g          387 t
ORIGIN      
        1 taatgtaaaa aatcatataa aaaaattaaa tttagtataa tatatataaa tatatataga
       61 aaggatgttt aaagtctatg agtggaaaaa ttttagttgt agatgacgaa cgtgatatag
      121 ctgatttaat agaagtctac cttacaaatg aaaactatat tgtttataaa ttttattctg
      181 gtcttgatgc actaaactgc attaatttaa atgaatttga ccttgcaatt ttagatgtta
      241 tgttgccagg agtaaatgga ttttctttat gccaaaaaat ccgagaaaaa tatacttatc
      301 caatcattat gttaacagct aaggatgaag aaacagacaa gattacaggc ttaactttag
      361 gtgcagatga ttatgttact aaacctttcc gacctttgga acttgttgct agagtgaagg
      421 cacagattag aagatataag aaatacagtg gaagtttaca atctggtgaa gaactttcac
      481 atattttttc ttttttaggt ctggaattga atacccaaac atatgaatgc catttaaatg
      541 gtaaagctct ttcacttact ccaacagagt tttctattct acatgtatta ttgaaaaata
      601 aaggctctgt ggtaaattca gaaaatcttt ttcatgctat ctggaaagat gaatattaca
      661 ataaaagtaa caatactata acagttcata ttcgccatct tcgtgaaaaa ttaggtgata
      721 ctgtagatga agcaaaatat ataaaaacaa tttggggtgt tggttacaag atttgacacc
      781 tatttttctt gtcaagaagg gatgtagtat tatgaaaaag attaataatt ttacttatag
      841 gaaactaaga gataaatata aatttataaa aagagaaaaa aatacagagg cgtatttaaa
      901 gtataaatta tatataatgt tagctatcta tacaggtata gtattggttt taatgtttat
      961 ggctgattat acgttttcaa atttttcaaa tcctcttgtt aaatggctta agttgagaag
     1021 agatgtatta ttttttttgg tttttataat tggatatatc actattttca atttttattg
     1081 gaaaaaacca tg
//
LOCUS       DAEPOG010000402         1004 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6041
            SAMN15890952-rid12467103.guided.333, whole genome shotgun sequence.
ACCESSION   DAEPOG010000402 DAEPOG010000000
VERSION     DAEPOG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890952
            Sequence Read Archive: SRR12506269
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067942.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067942.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:58:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,770
            CDSs (total)                      :: 3,720
            Genes (coding)                    :: 3,676
            CDSs (with protein)               :: 3,676
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 24 of 44
            Pseudo Genes (incomplete)         :: 18 of 44
            Pseudo Genes (internal stop)      :: 10 of 44
            Pseudo Genes (multiple problems)  :: 7 of 44
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890952-rid12467103.guided.333"
                     /strain="ZZV14-6041"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-04-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            199..831
                     /locus_tag="KR432_003770"
     CDS             199..831
                     /locus_tag="KR432_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0642241.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT          419 a          101 c          136 g          348 t
ORIGIN      
        1 agttttattg ttttgatata caatcaacta aacatataat ctttataaaa tactatacag
       61 ttaaaagtct gttttaaaaa aaaatgtata aattataaat caaaatatag atactatcaa
      121 ggagtgattc tgtgaagaga tgtattgtag tagtggaata gtcaaaaaca taatatatat
      181 taaaggagag atttaaatat gactattcca aattcaaata aggtatatcc aagaagtaat
      241 gattatcaaa ctgtatatct taaaaatgta ataacaaggg aaaatataga agttggagat
      301 tacacaatat ataatgactt ttataatgac ccaagagatt ttgagaaaaa caatgtatta
      361 tatcattacc ctataaacaa tgataaatta ataattggta agttttgctc aatagcatgt
      421 aatgcaaaat ttctaatgac tagtggaaat cactcaatga aatcaaaatc cacatatact
      481 tttcccatct tttatgaaga atggaaactc gatatcaacc atataacgga cgcttgggac
      541 aataagggta acattataat tggaaatgat gtgtggatag gttatgattc tataattatg
      601 tcaggtgtaa atattggtga tggtgcaatt attggaacaa gagcagtagt tactaatgac
      661 gtctcaccgt attctattgt tggaggagta cctgcaaaaa ttataaaaaa gagatttaat
      721 aatgatacaa tttcaaaatt attagaaatt aaatggtggg attggccata taaaaaaatt
      781 caatcaaaca ttaagcatat tcaatcagga aatattgata aattagttta atattttata
      841 atttatttat attaaacttt actttaataa tccttttata agagcttttt caaaatttgt
      901 atattatttt aatatatata aatttaatac aacattaaat aaaataaaaa gtaaagttac
      961 ttaataaaaa gttactttac tttttattat ttaaattact gttt
//
